Document ejxn22GG2pNrXB8VXEoxzedq

AR R26 - OL 83 MICROBICS MICROTOX TOXICITY TEST IESTSUBSTANCE~~~ 00000 Identity: Mixture containing perfluorooctanesulfonate which `may also be referred to as PFOS, FC-95, or as a component of FC-3003. Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not given. The test sample is FC-3003. Current information indicates it is a mixture of 1% PFOS, 15% diethylene glycol butyl ether, 79.39% water, 2% Sultone foamer, 1.5% sodium octyl sulfate, 1% sodium decyl sulfate, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data `may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD: 00 Method: Microbics Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1997 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes Statistical methods: EC50 values calculated by statistical linearregression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A 1 g/L primary stock solution `was preparedby dissolving 100 mgoftest substance in 100 mL of Millipore Milli-Q" water. The pH of the stock solution was then adjusted from 5.7 to 7.7 using 1.0 N NaOH. An osmoticadjustment `was made using 200 mg NaCl dissolved in 10 mL stock solution. Appearanceoftest solutions was noted as "clear". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Se-- Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss R0 ESOTS 00000 002693 Nominal concentrations: Blank control, 62.5, 125, 250, and 500 mg/L Element value: S-minute ECs = 311 (289 -- 335) mg/L 15-minute ECso = 171 (161 -- 181) mglL 30-minute ECso = 120 (118 -- 122) mg/L Element values based on nominal concentrations. SRuebmsatraknsc:e TReesmtianrgkwsafsielcd.onTduhcetveadlouenstrheepmoirxtteudraepapslydteosctrhiatbemdixitn utrheeaTnedstnot the fluorochemical proportion alone. c0 oncl0 usio0 ns 000000000 dTehteertemsitnseudbtsotbanec1e2300-mmgi/nLutweitEh Cas95fo%r Pchoontfiodbeanccteeriinutemrvpahloosfp1h1o8retoum12w2as mg/L. PSauublm,itMtNe,r:5531M33Company, Environmental Laboratory, P.O. Box 33331, St. p0 araQ0 uau0 ry 0000000000 tReesltiianbgi.liHtoyw:evKieirm,isscahmpralnekipunrgit=y2w.asThneotstpurodpyemrelyetcshaarlalcttheericzrietderaina dfotrhqeuasltiutdyy lacks analytical confirmation of the amount of fluorochemical proportion in the solution. R0 EFERE0 NCES00000000 TMhiinsnesstoutday,wLaasbcRoenqduuecsttednubmybtehre 3R1M33E1n,vi1r9o9n7m.ental Laboratory, St. Paul, O0 TWER 000 Last changed: 6/26/00. 002694 ENVIRONMENTAL LABORATORY: MICROTOX PREP SHEET eq lof LR: Date: R 133\ 3-13-97 Analyst: Ts TESTSUBSTANCE: ~~ FC-3003 AFFE (AUSTRALIA) SED RUN ------------------------eee 1. PrepaofrStaoctkSioluotinon +3FMiansaslVDoill.utTieosntVSuoblsutmaen:ce:||0_{q 0\Sg\ 10 mm| *Diluent: M1|\\ GD Pater *FinalConcenotfSrtoacktSoiluotionn:~~ 1g \ - 2. pHAdjustmentofStockSolution - * InitialpHofStockSolution:_S 1 *pH Adjusted 1: * pH Adjusted with: 4 Si drop 1-oN NaOH | 3.Osmotic AdjuosfSttomck eSolnutiton *Osmotic Adjuster: NaCl *Comments: _\Om\ of voc solidion wos added Yo 200vg Nac 4. Appearanceof pHandOsmoticallyAdjustedStockSolution . OPAQUE (circle one) "Color: leap *Comments: 5. Comments mr 002695 P32 of Row 2 FILE: R1331-2.K5 Second Run FC-3003 Test Time: b minutes NomsER 10/11 Coomnttrroal 9565..35967/ 0834..20)8 Lo wsises Toss 425 999298..313564///676304..794047 5 94.87/ 48.34 6 94.86/ 47.567 7 102.12/ 29.35 8 99.19/ 30.22 MICROTOX DATA REPORT Basic Test AFFF (Australia) 3-13-97 TKS Osmotic Adjustment: CONC. CR/GAMMA % BFFECT 00.000 00..58474253 8# sz.s000 0.141 8 12.4 11622250..50000000000 000...3132115885# a1904s.73 250.0000 0.679 # 40.5 250.0000 500.0000 0.711 # 1.986 # 4L.5 66.5 500.0000 1.817 # 64.5 NaCl AV % 11.3 24.5 41.0 65.5 - o 017. .: ; 9 1.0 qo. q U.& gq 1.0 #CK Us= edCoCfOoRrREcCaTlLeOaNlatFiAoCnTsOR = 0.8583 - EC50 311.0 mg/L (95% CONFIDENCE RANGE:289.0 10 334.7) Signature 25 TESTDATE: 3-13-97 TIME: cccaias anseme CONTINUED TO NEXT PAGE... g 002696 Poy Bet FILE:R1331-2.K5 PAGE 2 Rom 2 R33 3-13-47 Ts 10 32SDLDODPDEDD=DD1D.D2D7D9D6DDDLDDDPDDDDDDDDDDDDDDDDDDD. DLDDDDDIDDDDIDID3?3 Gc 33 a z 33 3 HHoo == 3 3 a33 J8 i3 1 >3sasaszezsasasnsasessantessss.o.t `5 33 So_6 3 3 3 4 --k 33 3 -- : 3 a, 33 3 3 2 EC50 33 -10 1@0DDDDDDDDDDPDDDDDDDD1"00DIDDCDODNLCDEDN.TRDADTDIDODNDD1D0D00DDDDDDDDIDDDDDDDD1D0DEY4 E95S%TICMOATNIFNIGDENECQEUATFIAOCNT:OR:LOG1C.07= 6003.776F9ORxECL5O0G b +2.4928 COEFFICIENT OF DETERMINATION: R2 = 0.99416 002697 Pq4 oC Ron 2 FILE: RI331-2.K15 MICROTBOaXsicDATTAestREPORT Second Fun FC-3003 ARF (Australia) 3-13-97 Tks Test Time: 15 minutes Osmotic Adjustment:NaCl NUMBER 10/11 CONC. CR/GAMMA % KEFECT AY Xo DIFF. CCoonnttrrooll 9868..3396// 867..7758 0[x .0 001.88990968 #u - 12 9532..5165// 6654..2290 6622..55000000 3h 99891.5346// 5513..4296 112255..00000000 55 9944..8667// 3332.12813 225500..00000000 78 19092..1132/7 11551.7338 550000.00000000 C#RUs=edCoCfOoRrREcCaTlTcOuNlatFiAoCnTsOR = 0.8952 00..236053 00..76173048 11..555817 84 44.l7s7132 w21.h3 mt g1.2 4401..16 40.9 90.8 8610..48 61.1 q 0.2 8822.18 82.7 q 0.1 KC50 170.8 mg/L (95% CONFIDENCE RANGE:161.0 TO 181.7) Signatur Sa CONTINUED TO NEXT PAGE: . EST TDAITMEE::32/3297 002698 . FILE:RI331-2.K15 PAGE 2 Pgs of 7 Row 2 Riza 3T-1e3s.97 10. SZSDLDODPDEDD=DD1D.D1D8D2D6DDDDDODPPDDDDDDDDDDDLDDDpDDIPNDDDDIDDDDDDLIDDD?5 3 Gc 33 ea--e 33 Jy 3 ra vo =: 33 A yo 5 LE=_ 33 3 1 3>zszzszazzarzsesszsansi ss.. . : 3 3 --i 3 3 33 -TT . 3 ~ i 33 3 33 Zonnl "kCs0 33 +10 @3UULODDIDoIGCDDDDDDYDD" DDD.: VDDIDDDDDDDDDY" DYDDDDDIDVIDDDDDDDY3 10 100 CONCENTRATION 1000 10E4 9E5S%TIMCAOTNIFNIGDENECQEUATFIAOCNT:OR:LOG1C.08=0805.843F0ORxECL5O0G b +2.2325 COEFFICIENT OF DETERMINATION: R'2 = 0.99691 002639 : fq Ce ex 1 : : Riss 3---~13-a% \s MICROTOX DATA REPORT v FILE: R1331-2.K30 Basic Test R aS Second Run FC-3003 AFFF (Australia) 3-13-97 TKS Test Time: 30 minutes NUMBER 10/11 Gone. Osmotic Adjustment:NaCl CR/GAMMA % EFFECT AV % gq DIFF. CCoonnttrrooll 9968..3b96// 993.33 .39 00.00 00.99467819 #8 - - 1 93.56/ 62.39 62.5000 32 9992..3165// 4662..3854 16225..50000000 4 98.54/ 45.93 125.0000 65 9944..6876// 2266..6510 225500..00000000 7 102.12/ 11.29 500.0000 8 99.19/ 10.50 500.0000 C#RUs=edCoCfOorRREcCaTlIcOuNlatFiAoCnTsOR = 0.9580 0.437 01..4005584# 1.055 # 22..441252 7.665 8.050 30.4 5281..83 51.3 7700..78 88.5 89.0 : 29.6 0.8 51.3 q 0.0 70.7 g0.0 88.7 0.2 EC50 120.3 mg/L (95% CONFIDENCE RANGE:118.% TO 1:i.4) Signatur CONTINUED TO NEXT PAGE: .. rest TparIe:M3-E[:3797_ 002700 . FILE:R1331-2.K30 PAGE 2 Pg 7 et 7 Riza] 3-13-q7 Tes 10 JZSDLDODPDEDDD= DD1D.3D8D1D1DDPDDDDDDDDDDDDDDDDDDDDDDDDDDDDIDDDDIDDDDIDIDD? 33 =. 3 =--_-- 33 MGA 333 [I] =Z-- I 333 a33 f. 1 >sszssasssssssozasass 4 _ 53 <3 35 ZT- 33 3 --- 3 z . 3 333 Lla .i 3 35 +10 @53DDDDDL_I-BDLDDLLDDD' D.EDCO5D0DDDDDDDDDDDDDD" DODDDDDDLDDIDDUDIDDY333 10 100" CONCENTRATION 1000 10a ESTIMATING EQUATION: LOG C = 0.7240 x LOG b +2.0802 C9O5R%FKCIOCNTFRINDTENCOEF DFEATCETROMRI:NAT1I.ON0:1758R 2 FO= R 0E.C9H09999 002701