Document eN6JqJpLGmyXDv9RX8yyGz14
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^^cletitijjic cd-ueuates
1474 S.Vandzviotsx Avb. Sr. Lock 10, Mo.
February 1, 1955
CERTIFICATE OF ANALYSIS
SDBJICTi Toxloologloal Invaatlgatlon of Pydraul F-9 (FH-103)
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TE'TS 00HD0GIED FGRi Hsnaanto Chaadoal Oompany, Maneaito Projeot Ho. SA-90
st. Louie, tteeouri.
5*'*
BTPERDSHTAL FROCBDOHEi
^_______ ;
A) Aottta Oral Toxialty
~~....
''X
Tha aral U^0 aaa found by tha introduotlon jif neaaurad single doaaa of un-
dllatad ooapound late tha atenaeha of Spragn*-I)aiflay (trainalbino rata by
aana of a rubbar aatbatar attaabad to a hypodatMLo eyrlnga.
iftar the approximate Minima* Lethal Ooaa aaa determined, groups of rata ear*
fad att lev*la daalpad to blanket the ^oxiolty ptnge thereby (applying data
far aalaulatlon of tha U^(.
/
Selected anlnale from thoaa euoounblng **r4.4utopsl*d and aaoroeoopla axsad-
natlon aada of the Tlaoara.
___ ,
Tba data ara ahoan la Tabla/J.
\
B) Toxialty By Skin Abaorptloh'
Undiluted Pydraul F-9 (FH-103) *" applied to the oloaaly ollppad intaot akin of albino rabblta In varying. amounts In/order to find tha Minima Lethal Doaa. Tha traatad area w*._a<iv*raa Vlth a plastic ahlald to retard aaaporatlon and tha anlrala aara.'blaoadUn apdoAeledgaa to prorent than fro* liald.ng off tha
J jTha reaulta i found in Table H.
C) Skin Irrltatlcinlla Babbit
Tha aaorlag naamd of Draft* Wbodard and calvary (J. of Phana. and tap.
Tbarapeutloe
Deo. 19UU) aaa uaad In evaluating Pydraul F-9
(FH-103) aa a akin Irritant whan plaoed on Intaot rabbit akin.
Oboervotlone aada over a period of several daya ara aoorad la Table III.
D) Eye Irritation In Rabblta Undiluted aonpound In tha mount of 0*1 nl. aaa plaoad In tha aonjunotival aac of the right ay* of aaeh of three albino rabblta and tba degree of Irritation aoorad aooordlng to Draiao, Woodard and Calvary (J.. of Pham, and Exp. Tharapeutiea, Vol. 02, Mo. 2, Deo. 19U4).
The reaulta are given la Table IT.
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036573
CONSULTANTS TO THS FOOD* CHBMICAL AND F H ABMACIUTICAL INDUSTBIBS
ii mil ohaaiMi
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St. U*a, MmouI.
lilwMfli taodtlN Qartlfloat* of luljtii - Pag* 3, tl5S
omonui munui (oaatin**d)
S) Tapor Inhalation Soar Baton Ml* rata r* plaoad In a anil **tal drua and aipoaod for ala boor* to a aatoratad ataoaphan of Ppdranl H faaaa by paaalag a atroa* of *aap**aa*d air tlrouib a oontalnar of the fluid plaood inaido tba obaBbor. Air pnaaaro u 16 poonda par aqaara inoh. Tba anlaiala wan obaokad for ojaytaM of toxlaity and all warn bald for a*a*n day* obnraatioa following UVOOBM. Tba data an oboon la Tahla t.
HONS 036574
1t IWto OhMd*al Oe any* t. Iml Ii HUoouri.
y,Matlfb lintlitoi Oartlfloat* of Anal7*1* - Pag*
2-1-55
I A B L c, I THi ORAL LD,,, OF PIDRAUL F-9 (FH-103) TOR LAXRATORI RAIS
dual Ho. - Sox
1- Hal* 2- Hala 3** Fcrale
It- Male S- Hala 6- Female 7- Female 8- Hale 9- Female 10- Hal* 11- Hal* 12- Mala 13- Fat-ale lit- Female 15- Fernala 16- Hal* 17- Mala IB- Hala 19- Mala 20- Hal* 21- Kale
Weight G
Do** On. / Kg.
Fat*
260
16.0
Survived
775
16.0
Surri*ad
215
16.0
. Survived
21t0
16.0 /\
Pied
230 16.0 \ \ Survived 220 21.0 \ \ Died
21*5 21*0 \ \survived
250 \ Died
195 220
21^0
'
Survived -x Survived
25C ___ ^ 2I*.0
^Survived
1.0
a-o
Died
20p
Survived
21*/> 2l/. G
Survived Died
*7.5
Died
27.5
Died
-27.5
Died
27S &.5
Survived n*d
DISCDSSIORt w* plaoad at's!4^'yra*a/*]
The Oral LD,- .
parAlloiTar. Survival tin* waa 00* to
eight da/a with
beh^praMiit 1A all anlnals. In all probability,
part of iba
tod rahatlToJy unchanged due to tba combination of
diarrhea
Appatltem affaotod but aald* iron the gaatro-
iatoatlaal
' * jetton* of toadoity war* obsamd.
norwal *zo*pt Ibr irrltitlon of th* digaative
HONS 036575
TO; HraMnto Owmlaal Company, It. Loale, Maaonrl.
atantlfie liMXirtai Oartifloate of Analyala - P(o It, 2-1-55 TABLE II
THE MINIMUM LETHAL DOSE OF FTDUAL L F-9 (FH-103) BI 3XDI ABSORPTION Might Change 5 Daya After
HONS 036576
To i Hanaanto Cheedoal On ly,
St. tenia, ftaaouri. MMtlfle Aaeoclsfa Osrtifloate of Analysis . page $, 2-1-55
TABLE in
310* IRRITATION IN RABBITS AFTER APPLICATION OT PUBAOL T-9 (FS-I03)
Animal No. - Sax
1 - Fanala 2 - MO* 3 - Fanala Average
Ihunrlaal Evaluation At The End Of 30 Ma. Bit Hours ||6 Hoars 72 Hours
2 1 2
1.6
3 2 2
2.3
DISCUSSION 1
Slight redneas alth practically no edaaa aaa preaai
sveragt aoors of 1.6 out of a poaaihla 6,0. The and aaa due to a alight lneraaae in ledneaa adth a tri third day the saapls bad nearly disappeared from the surfi
the aaerage asore had reduced to 1.6.
Pydranl 7-9 (FS-103) aae oonaldared to
irritant.
fclt IHF.I
TA BL
RABBI' F-9
APPLICATION
.Evaluation At The End Of mrs ItO Hours 72 Hours
DISCUSSION 1
The palpebral thirty adnotei aornea ear* hardly appeared to be
It 0 60 It 0 It.6 0.0
moderately red and showed a trace r.f edema within ore of 12.3 out of a posalble 110. The iris td The asore dropped to 9.3 overnight and all syea
HONS 036577
Ill BMb Cheat nil 0^4n7, It. IMi) HMMwrl.
MtartUil luadltn Certificate of Analyele - Page 6, 2-1-Si
TABLE T
TAPOR INHALATION <>' PTPRAUL T-9 (PH-103) BI RATS
Aninal Ho. - Sex
1 - Male 2 Mala 3 - Hale It Hale
Pate
Survived Survived Survived Survived
Concentration Of Pydraul T-9 Tapora In Charter
Saturated Atnoapbere
Average Temperature
Iaalde Charter
76.5'F.
Average Relative ibaidlty
Inal da Ouafaar
dG.oS
Discossiaii
Tho vaperlaed Pydrail T-9 aettlad quite rapidly on the fuir~o< th*s*nlrala and tfaa ehaWnr alii, Ocular and naaal lrrltatlop. cm moderate. BrWthbw baoame fairly haary and Irregular. Lethargy developed yfa aoBgrata extant aMe^ly after
capoanra began.
The artami a acre quite uhaunted whinA gained noratal activity and apatite ware no daatha either during or folloi
a obaeber but all bad oliowing expoaura. Thar*
MONS 036578