Document eKvaJRXYr5npvQZy1bvjk8j4

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredto as PFOS, FC-95, or as a component of FC-203FC. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-203FC. Currentinformationindicatesitisa mixtureof0.85% PFOS, 17% diethyleneglycolbutylether,74.46% water,2.34% acrylifcoamer, 1.7% alkylethersulfate2,.55% sodium octylsulfate0,.12% sodium nitrit0e.,09% sodium molybdate,0.85% triethanolaminea,nd 0.04% tolyltriazole. The followingsummaty applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemicalcomponent of the testsample. METHOD: Method: OECD Guideline203 Type: Acute statircange-finder GLP: No Year completed: 1997 Species: Pimephalespromelas Supplier:AquaticBiosystems,Inc.F,ortCollinsC,O. Analyticalmonitoring: Temperature,pH, and DO. Exposure period: 96-hours Statisticamlethods: Not given. Test fishage: Juveniles(128-132days) Length and weight: Average length Average weight Loading: 0.2936g/L Pretreatment:None Test conditions: 23 mm 0.09786 g (wet) Dilutionwater: Carbon-filterweedllwater(3M Well#2,St.Paul,MN). Dilutionwater chemistry:Not given. Lighting:Notgiven. Stock and testsolutionpreparation:The testmaterialwas added directly tothe dilutiownater on a weight/volumebasis. Concentrations dosing rate: Once. Stabilityof the testchemical solutions: Not noted. Exposure vessels: 1.2L vesselscontaining1.0L testsolution. Number of replicates:One. Blank controlduplicated. Number of fishper replicate:3. Number of concentrations: threeplus2 blankcontrols Water chemistry during the study: pH range(0-96hours): 7.9- 8.2(contreoxlposure) 7.9- 8.1(1000 mg/L exposure) Temperature range (0-96hours): 18.5'C Dissolved oxygen range (0-96hours): 7.8- 8.5 mg/L (controelxposure) 4.7 - 8.7 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control,10,100,and 1000 mg/L Elementvalues: 96-hourLC50=>1000mg/L Element values based on nominalconcentrations Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS... The FC-203FC 96-hour LC50 forfathead minnow was determined to be >1000 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability:Klimischranking= 3. The studyisa range-findingw,ithlimited exposure concentrationsand testorganisms. There isa lackof documentation. The sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemicalproportion inthe solution. REFEREN @ This studywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number R1335, 1997. OTHER Last changed: 6/26/00 ENVIRONMENTAL LABORATORY 96-M ACUTE TOXWJW TO FATHEAD MIN14OW, PINEPHALEs pRoAfELAs LR pg- otal TYPE TEST: STATIC V STATIC RENEWAL TEST SUBSTANCE: F-C -o?0:6 F'C, AV-F-F- DILUTION WATER- CARBON-RLTERED WELL WATER (31AWELL #2,ST. PAui.,MN) TOMP.-*C,PH -, D.O.-mo/L TEST FISH SOURCE: FISH AGE: NO. OF FISHffEST CHAMBER: WI)@5 DATE RECEWED@j AVMWT.:0.0'7'7%6,gtlmg "G. SIZE: ;21 OlS cm LOADING RATE: TEST SOLUTION VOLUME: /.OL- TEST VESSEL DATEltiMATED1:4-(,-4q-iT@eIs i oc;pArm IMML TOTAL CONC. AMOUNI 2444R pH 49-HR mg& ADDED m,9,/oL* I '7.9 o IS.1 1 0 SIZE OF TEST VESSEI.S: L DATETEFOMATED. 14-1 -9 -7 1 @ac> MORTALITY pH D.O. 7244R pH D.O. 96-HR pH D.O. MCWL 1.91 7-o 0 ".,4 To. o I mg/L I ma/L 19.Cl> 7,-l 7.9 12.1 18.5-. c> I t,?- ir,44 5 A 10 1 to 1 8.5 ir.c. 6A 77AAri o lw"lr C) 1-1.-1 0 -1.'9 L4.-1 1 1 I I I 1 IS.Z)17-9 1 0 1 t.1 1 I (..g' 1 TEMP. -C DATE / ANALYST LCSO (96% C.I.) (5-c@ -1 11o c)ikrn -CkS L4 -1 <.100 0 cl-7 -ri<-5 (ESIDC .9 44-18 ot-1 -(e COMMENTS SECONDARY ANALYST: 2%1 C) 'n@@ hmkebi ()C)ee-- -j rec.A&"4.6'%n U-,o>kr W2"Av@@.k5'%Ay%'ri%Q. C.0 fQC. -7A Wet-% Jf"*-moxqit ov-% Li-l(,-CI,7ac., 4@, to 4n tooowtAl(-) EFFECTS: S m Surtachg-E LostofEquNftum;0 QU"cont R EffdcSwknrringH; Hanwrhaging --Tic5 Environmental Laboratory LR: Date: +-Ir-9-7 Initials:-Tqs Page of WHour Acute Toxicity To Fathead Minnow, Pimopholes Loading Rate Data Shoot Toot Substance: o@o Fc- KFFV Promolas Fish I 2 3 4 5 6 7 10 Averso Weight, (a) o. 09((, 0,05,Lz o - 17-:3.3 Length,(cm) -H 1 .1? o.o?iz o. o C)HL 0,0(0(0/ 0 coza 0.2 ot 1 '2.5 2.2. 2.(0 Landina Rat& Calculatlan - Avg. wt. (g.): 0. 09-7?6 Std. Dev: o C) 9 No. fish/vessel: 3 Vol./vessel: 4.- 0. 2,1135 g. fish I Loading rate: