Document e7aEjj3B5pD55K08dNvdbxk0m

/.J*- R I V I J i^tu'.<r3<oc>. E. I. du Po nt de N emours S C o m pany i n c o r d o * t c o W il m in g t o n , D e l a w a r e 19898 POLYMER PRODUCTS DEPARTMENT kX PtH lM E N T AL STATION PERSONAL AND CONFIDENTIAL DR. R. J. CLAYTON MEDICAL, WILMINGTON cc: A. J. Dahl B. W. Karrli L. J. Papa Pral File I .C. - 353 - N11400 - 29 AHtZ>- tsq5 August 19, 1981 ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTANOATE (Job No. 810-866; PRAL Nos. 81-3517, 81-3520-3521; Notebook Nos, E22514.E26238) As requested in you letters of 8/6 and 8/10/8L, the 3 blood samples submitted then have been analyzed for perfluorooctanoate (Cg). Results and sample identification are given in the attached table. As noted there, the analyses were done using a gas chromatographic method specific for Cg (Lab Method Number ES-567) but results have been reported as ppm F for comparison with total organic fluorine analyses. Precision is + 10L relative standard deviation over most of the concentration range, somewhat less at the lowest values. The lower limit for quantitation is 0.007 ppm F (0.01 ppm perfluorooctanoic acid), with a detection limit of "j 0.004 ppm which can be distinguished from the reagent background but not well quantitated. Please contact me (772-4440) or L. J. Papa (772-2745) if you have any questions regarding the analyses. General questions on blood sampling can be directed to J. W. Raines or L. F. Percival. Attachment jah Key Words: Perfluorooctanoic Acid Perfluorooctanoate Blood Analysis GC S. S . Stafford There's a w orld o l things we re doing som ething about 000123 EXP000047 E ID713861 I r TABLE I CONCENTRATUl-i P/. J.N JJPP.D (a) Sa-ple _______________ PRAL So".' 81-3517 81-3520 81-3521 8/6/81 8/10/81 8/10/81 P.R.No. Kane GC Analysis Date Analvzed 8/11/81 8/12/81 8/12/81 tg J 0.043 0.098 0.33 (a) Analysis as described in Lab Method ES-567 ("Determination of Perfluorooctanoic Acid in Blood, Gas Chromatographic Method", S. Stafford, 4/3/81), using the packed column GC analysis with perfluoro-n-octanoic acid as calibration standard. (b) Although the analysis is specifically for perfluorooctanoate (acid or salts), concentrations are given in ppm fluorine for comparison with the results of total organic fluorine analyses. (ppm F = 0.6S8 x ppm perfluorooctanoic acid) Estimated uncertainty is + 10% relative standard deviation. The lower limit for quantitation is 0.007 pgF/g. The detection limit is 0.004 VgF/g, but concentrations in that range cannot be well quantitated and are reported as < 0.007. None detected (n.d.) is reported for samples with [Cfl] < 0.004 ppm. which cannot be distinguished from reagent background. 000124 EXP000048 *REDACTED* EID713862