Document e7Y41RdEYZO9Ny2QmLjy8008e
AR 226-0515
MICROBICS MICROTOX TOXICITY TEST
TEST SUBSTANCE
Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to `FaCs-P11F6O,AFaC-m1m2o6n,iFuCm-1s6al9t,, AFCm-m1o4n3,iuormapseraflmuaojroorocctoamnpoaotnee,nPtFoOf, FX-1003. (Octanoic acid, pentadecafluoro-, ammonium salt,
CAS # 3825-26-1)
TRheematrekstss:ampTleheis3FMX-p1r0o0d3uc.tiIot'ns lpoutrintuymwbaesrnwoatssu2f3fi2c7i.ently characterized, though current information indicates itis a solution of <45% ammonium perfluorooctanoate, 50% water, <3% inert perfluorinated compound and 1 ~2% Cs and C7 perfluore analogue compounds.
Ttehset fsoulblsotwainncgesiunmwmaatreyr saoplpultiieosntwoitthheitnecsotmspalemtpelleyacshaarmaictxetruirzeoedfthe ofcotnhceenttersattsioanmsplofe iwmiptuhritthiaets.oftDhaetatemsat ysunbosttaanccceu.rately relate toxicity
METHOD:
Method: Microbics Microtox "BASIC" Procedure
Test type: Static acute
GLP: No
YSepeacriCeosm:pPlheotteodb:ac1t9e9r0ium phosphoreum
Analytical monitoring: Light loss.
Exposure Statistical
period: 30-minutes methods: EC1o and
ECso
values
calculated
by
statistical
linear
regression program provided for Microtox
TTeesstt ocorngdaintiiosnmss:ource: Not noted. Diluent: 2% NaCl Microbic's Reagent. Test temperature: Not noted.
Stock and test solution preparation: A 2000 mg/L stock solution was prepared in Millipore Milli-Q water. Test solutions were prepared using
aliquotsofthe stock solution. Concentrations dosing rate: Once Stabilityof the test chemical solutions: Not noted
ENxupmobseurreofvreespsleilcsa:teMsi:crtowboic's cuvettes.
Number of concentrations: Four plus a blank control
03880
RESULTS
Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L
Element value:
5-minute ECso = >1,000 mg/L.
1350--mmiinnuuttee EECCsso == >>11,,000000 mmgg//LL.
All element values based on nominal concentrations.
Rdeesmcarrikbesd: iTnetshteintgeswtassubcsotnadnucceterdemoanrktshefimeildx.tuTrheeofvatlheuetsesrtespuorbtsetdanacpeplays
to that mixture and not the test substance.
CONCLUSIONS ~ -- The FX-1003 30-minute ECs for Photobacterium phosphoreum was determined to be >1,000 mglL.
`PSauulb,miMtitnenr:es3otMa,Co5m5p1a3n3y, Environmental Laboratory, P.O. Box 33331, St
DATA QUALITY
tReesltiianbgi,libtuyt: lKacikmsisancahlyrtainckailncgon2.firTmeasttiionng omfeteetstssalulbcsrtitaenrciaefcoornqcueanltirtaytions.
There is a lack of characterization of the test sample.
REFERENCES TMhiinsnesstoutday,wLaasbcRoenqduuecsttednubmybtehre G32M88E2nv.ironmental Laboratory, St. Paul,
OTHER
Last changed: 5/25/00
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ENVIROMENTAL LABORATORY
MICROBICS MICROTOX SYSTEM
RA ATA
woABEL
pati
!
rest suswnee: _EX=1003 lot A327
ANALYST:
oo
SALE CWETTE
A. Loach mg,
.
son. | ToRR-| 30-HIn.
1(0
1(8 1015) 1(30]
a
DUPLICATE
04 |85 |73 73
5
125
log |79 [64 | 6A
@
Doplicade
Jog | 78 |bb | 6A
ws
Ase
Jog | 76 |bH |54
ce
Duplicate
los | 74 | LO |S56
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1o06 | 74 {LO 5526
5
Joeo
jog | 5 | Ha |H6
cs
Doplicade
log | b7 |8& [Ha
SoCong Whi ale Bal Teor
pr
Cobionc5 e
liga.
Jrock soln, = 40comall Fu1.c 0 mLk=s 2.0
Test Shstone cored iserble win Mili- Ber.
o
Tniviol pht= 6.8, adyvsted. to pHb7 wis diluely
oh Adjosinen; Adda zoo ry Mack to fo nL Sample.
Diluens 5
pisst Solon Clear, Colorlesy
A
`robes 2% Nak Solotions
03882
Tita Dl vrtioonnss, Znivisl Contenitations Were AevbletheSirad /
tows 8+.
MICROTOX (r) DATA SHEET Microtox S-min exe Fx-1003 LR 62882 3/7/90 sab
Par 4 CONC.
321 122155205...000000000 sa 2S5000..000000 7s S#0100.000.00000 8 %1000.000
BBLLAANNCK RABToI/OB=t=0.8110000
SC SO = 29347.118
to/1t
1110008991..000777 777896...000 110151..00/7 7746..00 1061.008/.0774.605.0
109.07 67.0
/- 81
.
-
%2565.755 TO
6-088
o00l..111216282 00..114893 0.01.63045
0.318
%34051.820 )
R =0.95295 SLOPE = 1.9111 INTERCEPT = 49.1428
paz X4 .
Gest
o00l..111i15224 00..125141 ol02.12189
0.289
Li%an Legg
I111 .\ a:b 24
W_eghs yoo
| +Gdos
ECso ) 1000ma |i
Wh1 0R.E4S2.0 PROB5.7 J :: ::
25 00.249002 2575 ::
::
5 0o.2n79 2315 ::
LLoL
76 00..528952 120%0 -1>:
7.`e
8 0.302 82 :
--eeee-8-l 6
;::
meme osmm mmCLomwAC
2:: mmm mFeneamme `L: iosa
fmm mmm
:
5 S:: nirmerogm A san ra ::
4
5 LOG CONC.
6
7
MICROTOX is a Registered Trade Mark of Micrabics Corporation.
93883
HICROT(O+X) DATA SHEET Microtox 1S-min exs. FX-1003 LR 62882 3/7/90 sab
Paz eft
PAIR " conc.-
TT 125.300
2
125.000
3
250.000
sa S02500..000000
6
500.000
87 7#11000000..000000
sBLLAANNKK RBAoT/IBOtS=0.7110000
EC So = %3347.798
toorte
109707 63.0
108.0/ 66.0
109.0/ 64.0
110150.07/ 6630..00
106.0/ 60.0
110089..00// 4592..00
/ 71
.
_
( %1084.118 TO
Goss
57555
0.162
0.209
ol0.a24:2
0.254
0.0.546858
%10338.120 )
R =0.90172 SLOPE = 1.7262 INTERCEPT = +8.1161
G-esT
se
0.167
0.231
00.322311
0.321
00..444444
Lign%e LoseTM>
ee
"
7
290
20
3343
0
:
_GobS yp
1 +Goks.
ECs, > I00O ros
ow1 0R.E4S0.0 PROB3.3 1 A:: men
523 0oo.ll5a4oe6o S&s ools3amr 78 oollseas
625325
iI :
22a2 o>:
1070 ::
i:
as:i
Ym nme
perme menti : 4i[a <i6c :le
70lma mnt,on4 mmm? 3
4i
fmmmmmee--meenntSeeeemermnnt,
.:
JO A
4
s Los conc. 6
7
MICROTOX is a Registersd Trade Mark of Micrebics Corporation.
03884
HICROTOX(r) DATA SHEET Microtox 30-min exe. FX-1003 LR 62882 3/7/90
Pak 0 conc : torte
6-088
321
211522055...000000000
111000998...000/// 566922...000
4
250.000
105.0/ 56.0
5.
550000..000000
111016..00/7 6506..00
78
%#11000000..000000
110089..00// 4469..00
000...322145287
0.331
00..33193
00..567679
sab
Gest
000...232303822
0.308
00..4400%9
00..554444
Light LossIID
21> 14
2
a2H6
3Y7o
BBLLAANNKK RBAoT/IBOtS=0.71.0100Q / 71
-
_
EC SO = %2918.197 ( %1094.210 . TO %7762.670 )
R =0.91498 SLOPE = 2.0386 INTERCEPT = 47.9787
--we5, hS 100
14Goks Eg > 1000malt
e+1 o.Reass.s PROB8.
| i mmm :
mm mmm mmmi: ;
32 o0.l3a3m s2s7 :: 3 0.319 24 :
:PL
s6 o.0.314 7 olea7
226a o>; 100 i
<io6 :
8 ols 70 :::
[Rlrs . mn
co::
eme mmmml mnl A JE ------ <s
::
ri mme m snlm
:.
:{omen lgemeemeee e
::
Te 4
wrsenLmosmaconncm.mpu6pmmmsnhmaenrsnsmy7
HICROTOX is a Registered Trade Mark of Microbics Corporation.
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