Document e75xZnLLgjDOyjyBM93aoM2Y4

AR226-3124 DuPont-3104 HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE H-24119: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna PURPOSE: This is a rangefinding study designed to provide an estimate o f aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. GENERAL INFORMATION WORK REQUEST NUMBER: Ifl HASKELL NUM BER:--^ SERVICE CODE: MATERIAL TESTED :! S Y N O N Y M S /C O D E S :l7S' SPO N SO R t^ JPuPont Specialty Chemjca NOTEBOOK ID: 1f l SUBM ITTER'S NOTEBOOK N U M B E R : r ^ ^ ^ |^ 5 SIJtby sponsor COMPOSITION:! H P / ,J , CONTAMINANTS:t-Residual SClvent (THF or MIBK). Residual ammomum hydroxide fiom _ .neutralizatj CAS REGISTRY PURIT MSDS No: Not supplied by sponsor TEST CONDITIONS Nominal concentrations tested: 0, 0.5, 1.0, 50, 500, and 5000 mg/L. Nominal Concentration (mg/L) 0 0.5 1.0 50 500 5000 Test Initiation (0 hours) Dissolved Oxygen (m Jb PH Af Bf At 8.6 8.5 7.1 8.8 8.8 7.1 8.8 8.8 7.1 8.7 8.8 7.1 8.7 8.7 7.1 8.7 8.6 7.1 B1 7.1 7.1 7.1 7.1 7.1 7.1 Test Completi on (48 hours) Disselived Oxy gen (m /L) _ At B1 ________ E]H A+ 8.9 8.8 7.7 8.8 8.8 7.7 8.8 8.8 7.7 8.8 8.8 7.7 8.8 8.8 7.6 9 .2 " 9 .2" 7.1 " Bt 7.7 7.7 7.7 7.7 7.6 7.1 " t a and B represent replicate test cham bers within a dose level concentration. a M easurement taken on day 1 (24 hours) due to total immobility. Page 1 o f 2 Company Sanitized. Does not contain TSCA CBI DuPont-3104 H-24119: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna, CONTINUED RESULTS Based on visual observations, the water control solution and the 0.5 and 1.0 mg/L test solutions were clear and had no color throughout die test. The 50 mg/L test solutions were clear with a suspended substance, die 500 mg/L test solutions were slightiy cloudy with a suspended substance, and the 5000 mg/L test solutions were cloudy and white in color with a suspended substance at test start The suspended substance in the 50, 500 and 5000 mg/L test solutions setded onto the bottom of the test chambers as a white cloudy film throughout the remainder of the test. Immobilities were 0, 0, 0 ,0 ,1 0 , and 100% in the 0,0.5,1.0, 50, 500 and 5000 mg/L test solutions, respectively. Forty-eight hours after exposure, lethargy was observed in 20% of the remaining mobile daphnids in the 50 mg/L test solutions, and 33% (3/9) o f the remaining mobile daphnids in the 500 mg/L test solutions. The 48-hour ECSo, based on nominal concentrations and immobility, was between 500 and 5000 mg/L. All water quality parameters were within acceptable limits. CONCLUSIONS H-24119 exhibited low toxicity in a 48-hour, unaerated, static acute test using Daphnia magna. W O R K /A U T H O R E D B Y : Q r f S t J l J J ' K k - - ____ Lamie D. Bouchelle Toxicology Associate |% Day-Month-Year Page 2 of 2 Company Sanitized. Does not contain TSCA CBI