Document e5no1Go0kq6mRERQv2vyQJELy
Revolt N. Baer, Ph.D.
Study Completed On September 4, 1991
Performing Laboratory E. Z. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine
Elkton Road, Post Office Box 50 Nevaifk, Delaware 19714
Laboratory Project ID Haskell Laboratory Report No. 395-91
i
[Page 1 of 14 !
,tcontainTSCACB Sanded. Dees nd .company
?j
This study va? epndu Agency's " " non-conplience ere effected the veXidity
Submitter; %, I.
i Sponsor;
Study Director;
J>
I a
$<3&pm San ^ ^ ROtCm a m " 'W
iiML
STUDYI
T j T J ^ I E, 96-HOUR LC50 OF TO FATHEAD WINNOWS
tSpnaes promelas)
AUDITS:
Items Audited In-life Observations Protocolp Records ^ and Final Report *
ludit Dates
15/15/91 j 17/22,23/91
DATE FINDINGS REPORTED TO MANAGEMENT AND STUDY DIRECTOR: 7/23/91
Reported
lantill jitor Quality Assurance
'Date
l
W -3-
Du Pont Hl* 395-91
oo co eo go eo a* o\
V. Materials and Methods . . A. Test Material . . . . B. Dilution Vater . .. C. Animal Culture . .. i D. Test Methods . . ..' E. Chemical Analysis ..' F. Statistical Methods . j
I. VII. VIII.
Results and Discussion . \ ..................... ............ 9
i
C onclusion............;............ . ..................... 9 ].
Literature Cited . . . . ..................................... 10
IX. Tables
1. Chemical Characteristics of Haskell Laboratory Veil Vater
2. Vater Chemistry of the Dilution Vater eOteSt Start^
3. Physical and Chemical Characteristics of
Test
Solutions
4. Observed Mortal
fathead Minnows ( _ es promelas)
Exposed to Aerated Test
dr 96 Hours in ia i t l ^ T ------
li 11
12
13
14
-Conmany S a n * - 0 = KolconWn TSCA CBI 4
J2axb
W-.
',:i54l5
. "
Du Font BLR 395-91
If
not acutely tfHEiU to J^ihf|ei^;M i e a d einnowg C|
promelas) in a"*S-hour, aerated* itatic test. Hii1^_test concentrations were
648, 1^0, 1800, 3000, and 5000 ( ;/L. The BiMfipjt' 9 ^ based on noninal
concentrations vas greater than
ag/L, the hifhip concentration tested.
Vork by V. Troy Technic:
er
Tl.
UlSpisi-
R. Scott Vivian
Technician '
6 ht h i Mo/DayvYear
Mo/Day/Year
Robert V. Petersotnn Technician
Mo/Day/Year
) Wallace B. Fossicks
^//9/ Mo/Day/Year
Technician j
Study Director!
---
Kevin N. Baler, Ph.D.
Research Toxicologist
Environmental Sciences
Mo/Day/Year
THIS REPORT IS APPROVED FOR i s s u e |.
cevi/in Hi .-'ilerr PhD
/Study Director
f/ d iL
Mlo/oD/aDiay/Year
S a n d e d . SoeS
isivsarwtTrStfcCBI
5
"VTfWSSS
I
*,*w4* % M
Medical s'"!"iilpt^
Haskell^g^fel Haskell Laboratory Test li'l
Other
i S Synonyms* Purity*
Composition*
L1JII4Pu Pont BIUR 395-91
-affjC
,j / 1- ii*,- .
r
' .. JW .iy**i 3
ii j'lljj
'ii
''$$)\Wi 71V
,I
Contaminants*
Sponsor* Du Pont
I?*-Z,m .Pont
Wilmington,
Nemours and Company 19898
Submitted By:
a
Du Pont chemioals Chambers Works New Jersey
Study Schedule:
study iBiitiatWl :|fc* 5/14/91 Experimental flgjf| ffte* 5/15/91 Wxperieftiai T^lil|g|ion Date: 5/19/91
study iblyletiol''# ^ : 9/4/91
Notebooks*
Distribution:
\L-~'
6
The goal of juvenile, " `
''
All date and the fine! Be Laboratory for Toxicol
archived at the Ou Pont
m |II1
P Pont 9LR f^-ei'' icity of
ther at Haskell k, Delaware) or be ngton, Delaware).
J
I
~
I
f -tt
TSCA r 'K Uzed. Does not conM^ Company Sam
T? ^1
?. MATERIALS
Du Pont BLR 395-91
. Test Material
Source, composition, end pun ty Of th test General Information. She approp;fiate quantity dispensed into 4-L of dilution vif ter (see " concentration. Stability wad so' ability e not determined.
described under
test in dilution water were
B. Dilution later
later (Table 1) originated fi[om the Haskell Laboratory veil.
C. Animal Culture
Fathead minnows (Plmephales irpmelas) were reared in continuously flowing well water at Haskell Laboratory! Adult fish resided in 3B L aquaria at approximately 25C and were fed frozen brine shrimp (Artemia sp., Golden Gate Brand, Korden Division, Novalek, jlnc., Hayward, CA) generally twice dally, once on Saturday, and not on Sundays, j Eggs were deposited on polyvinyl chloride tiles coated with sand. Tiles vilth eggs were aerated and incubated at approximately 25C in a water bath. Larvae were reared in 22 L aquaria at approximately 22C and were fed nlevly hatched Artemia generally twice daily on weekdays and daily on weekends. Juveniles were held in a 22 L aquaria at approximately 22*C and were fed nlevly hatched Artemia and/or frozen brine shrimp generally twice daily on Weekdays and once on Saturday and Sundays. No treatment of the fish for-diseases was required during the holding period. Sickness, injury, and abnormality were not observed. Animals were identified by labels on aquaria and test vesjsels.
D. Test Methods [1]
Five test concentrations and a water control were used. Nominal test concentrations were 648, 1080, 18)00, 3000, and 5000 mg/L. The fathead minnows ranged from 1.5 to 2.4 cm in standard length (mean 1.9 cm) and 0.029 to 0.16 g in weight (mean 0.092 g). The fafthead minnows were 85-86 days old at test start.
Glass aquaria (5-L) containing 4 L of test solution (approximately 12-cm
liquid depth) were employed. Two! replicates of each test concentration with
ten fish per relicate were used. jFish were added to the test concentrations using random numbers and were not; fed for 48 hours prior to the test nor during the test. Mean loading for controls at test conclusion was 0.23 g/L. Test
solutions were held between 20.7 And 21.6C (mean 21.2C) and were unaerated, until at approximately 72 hours when low dissolved oxygen levels necessitated the use of gentle aeration (approximately 120 bubbles/minute). A photoperiod of 16 hours light (215 Lux) versus 8 hours darkness was employed.
j. Do^s orspaii Ssn
8
sj
i.
"t-
Du Pont BLR 395-91
Dissolved ezygen CfSI
^Jtostruinents, Tellov
Springs, OH), pH (Coring ..
fie Instrunents, Hedfield,
HA), and temperature (Americaft.,
erlals registered mercury
sten thermometer) were neiwMPp1
Rations rad the vater
control at the beginning of t!
of fish) and dally
thereafter. Total alkalinity
1 Company, Loveland, CO),
EDTA hardness (Hach kit, Httch
and, CO), and conductivity
(YSI Model 33, Tellov Springs 1nstruneot, Tellov Springs, OH) of the dilution
vater vere measured at the begi ' _ of the tea## fetal organic carbon (Model
700 01 TOC analyzer) wa.s aeasu_r'fed in the dilution vater at 48 hours.
;
Cheeical Analysis
Chemical analysis of the test material was not conducted.
F. Statistical Methods
The 96-hour LC50 p-1 its associated confidence interval could not be calculated because mortality vas not observed at any test concentration.
t i . Results a n d Discussion
A 96-hour rangefinding study vas conducted using the folloving concentrations; vater control, 1.0, 10, 100, and 1000 mg/L. Mortality vas not observed at any test concentration.
J Nominalj B | B H B ^ c o n c e n t i a t i o n s vere 648, 1080, 1800, 3000, and 5000
mg/L. All test concentrations (excluding control) vere cloudy throughout the
testing period.
All chemical and physical parameters (Tables 2 and 3) vere vithin expected ranges, except dissolved oxygen!in replicate A of the 5000 mg/L test concentration at 72 hours. Thejdissolved oxygen value (5.3 mg/L) dropped slightly belov 60% of the theoretical saturation value of 9.0 mg/L at 21C. As a result, all test concentrations vere gently aerated beginning at 72 hours (approximately 120 bubbles/minute). This apparent decrease in dissolved oxygen vas not considered biologically!significant based on the lack of observed mortality. No mortality or sublethal effects vere seen in any test concentration (Table 4). The 9fe-hour LC50 based on nominal concentrations vas greater than 5000 mg/L, the highest concentration tested.
fll. CONCLUSION
[vas not acutely toxic to juvenile, fathead minnows (Pimephales promelas) in a 96-hour aerated, static, test. The 96-hour LC50 based on nominal concentrations vas greater than 5000 mg/L, the highest concentration tested.
iO'*-fx 9
`iE
i
v'iki S
flilil;J ' i'.";' l
Du Pont HUI 395-91
Chemical Characterist '.B o#Hask9li fel>Qg#ory U ater*
Parameter
Concentration
11. ... `"
-
Parameter
Concentration
BOD3, mg/L
<2
COD, ng/L DOC3, mg/L TOC, ag/L Kjeldabl N, mg/L
Ammonia N, ng/L
13 1.5
<0.5!
O.* <0 .(
Turbidity, NTU
Phenolics, mg/L
Color, Co/Pt
<2Solids total suspended, mg/L
Aluminum, mg/L
<0.1
Antimony, mg/L Arsenic, mg/L Beryllium, mg/L Boron5, mg/L
<0.060
<0.010 <0.001 <0.020
Cadmium, mg/L
<0.0|02
Calcium, mg/L
25.0!
Chloride6, mg/L Chromium, mg/L Cobalt, mg/L Copper, mg/L Cyanide, mg/L Iron6, mg/L Fluoride6, mg/L
6.3
<0.010 <0.020 <0.010
<0.025
0.053
<0.1
Le$d, mg/L Magnesium, mg/L MBAS/LA5, mg/L
Mercury, ng/L Nickel, mg/L Nitrite6, ng/L Nitrate, mg/L Phosphate6, mg/L Potassium, mg/L Selenium, mg/L Silver, ng/L Sodium, mg/L
Sulfate6, mg/L Sulfide, mg/L Thallium, mg/L Zinc, mg/L Volatile priority
pollutants Acid extractable
priority pollutants Base/neutral
priority pollutants Pesticides/PCBs Organophosphate
pesticides7, yg/L
<0.005 3.r
<0.05 <0.00020 <9-r020
<0 . 1 1.6 <0 . 1 1.8
<0.005 <0.310
8.1
4.8 <0.05 <0 .0 1
<0.020
ND
ND8
ND* ND*
<0.50
iDat of analyses 17 October 1990 unless indicated otherwise, analyses performed at Environmental Testing and Certification Corporation, Edison, New Jersey; 3Date of analyses 13 December 1990, analyses analysis performed at Environmental Testing and Certification Corporation, Edison, New Jersey; Mean value; Date of analyses 17 May( 1991, performed at Du Pont Engineering Test Center, Newark, Delaware; 5Belcjw the Published Method Detection Limit (BMDL); Date of analyses 9 November 1?90, analyses performed at Du Pont Chemicals, Jackson Laboratory, Deepwater, New Jersey; 'Date of analyses 23 October 1990, analyses performed at Hazleton ILaboratories, Inc., Madison, WI; None detected.
. . .. , ..^esnoeenttnTSCACW company saniurw- -oes
11
1- *
r1 % J t |
.
' -<. - ",;11 ^ f : ;;ri- V"
.1
A
v; i Du Pont BLR 395-91
rm 3
>:
Physical and Q |ei||l Charecteristics of
>. - -.*-J-.;-iJ!<j *V
"
''. |
... '1
'
NOBilisl^Cne|Rtrations
*
Tie (flours)
Vater Control
A B*
648 A* B-
Dissolved oxygen (ng/L)
1080
A* B*
-
1800 A* B*
3000 A- B*
5U>0 A* B*
Start
8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.7 8.7 8.5 8.6
24 7.9 7.7 7.7 7.4 7.4 7.6 7.2 7.7 7.6 7.5 7.8 7.7
3 48 7.7 7.7 7.2 7.3 7.1 6.9 6.4 7.1 6.6 6.4 6.7 6.0 72 7.6 7.5 7.2 7.3; 6.9 6<8 6.7 6.8 5.9 6.2 5.3 5.5
96 8.2 8.0 7.7 7.6 7.3 7.2 7.6 6.9 6.3 6.7 6.1 6.7
pH
Start
7.3 7.3 7.3 7.3 7.3 7.4 7.4 7.4 7.4 7.4 7.3 7.4
24 7.1 7.2 7.2 7.3 7.3 7.3 7.3 7.3 7.3 7.3 7.3 7.3
48 7.2 7.4 7.4 7.4 7.4 7.4 7.4 7.5 7.5 7.5 7.5 7.5
72 8.1 8.1 8.2 8.1 8.1 8.1 8.0 8.1 8.1 8.0 7.9 7.9
96 8.3 8.3 8.2 8.2 8.1 8.1 8.1 8.0
--
A and B refer to replicates t^ith ten fish each.
8.0 8.0
8.0 7.9
mj w
Company Sanitized. DoesnotcontainTSC.ACp
13
Du Pont BLR 395-91
Observed Mortality of Pa
for 96 Bow
(ag/L)
Vater Control 648
1080 1800 3000 5000
Percent emulative Observed Mortality*
24 Bours
Ab Bh
00 00 00 00 00 00
48 Bours --
!Ab B
j
0 0 i !0 0
00
00
00
o0
72 Hours
A* Bb
00 00 00 00 00 00
96 Bours
Ab B
00 00 00 00 00 00
All fish appeared normal. bA and B refer to replicated vith ten fish each.
ppflpafl'S'*'ifes^esB
tntSCAkCt
14
