Document e5NZV09nnaMDgkv30JKX0x36q

ISTirUTO DI RICERCHE FARMAtOLOGICHE "MARIO NEGRI,, FCNT>.*JZIOS" PER RICERCHE ERETTA LN' E.NTE MORALE CON D. P. R, J61 DEL 3 APRILS 1961 - REG. PERSONS GIUR. HUS. MILANO N. 162, VOL. 3 CONTO CORRENTE POST. N* 3/39071 RECOGNIZED AS A TAX EXEMPT ORGANIZATION UNDER SECTION 301 (e) (3) OF THE UNITED STATES OF AMERICA INTERNAL REVENUE CODE ! ETHYLENE DICHLORIDE Report on the clinical-chemistry results after 5 months inhalatory exposure First Report Milan September 1976 gbp/ 20137 MILANO - Via Eritrea, 62 Tel. 33.34,346 - 33.70.546 Telex 37268 NEGRINST SL 070^20 / 1- The following is the report on the clinical chemistry data obtained after 3 months of exposure of rats to inhalatory Ethylene Dichloride (ECD) . Animal (Sprague-Davley rat of both sexes) were treated in Bologna by Dr.. Maltoni according to his protocol employing 4 levels of exposure , i.e. 250, 50, 10 and 5 ppm. Controls (of the same strain and age) caged in similar conditions , breathed only air . Blood samples were obtained by heart puncture of ether-anesthetized animals: the samples were coded and read blindly, the code being broken only after tests performance . Animal Nos. 801-810 correspond to female controls and Nos. 847-853 to male controls; Nbsl-8 to females and Nos. 46-53 to males exposed to 250 ppm ECD (hereafter called group 1) . Rat Nos. 201-208 were female and 246-253 were males exposed to 50 ppm ECD (group 2) ; Nos. 401-408 were female and 446-453 were males , exposed to 10 ppm ECD (group 3); Nos. 601-608 wTere females and Nos. 646-653 were males exposed to 5 ppm ECD (group 4) . The selection of the animals, nunbering etc wore performed in Bologna ,this group having performed the analysis on- the samples submitted . The following analysis : BNU ; SGOT, Biliruibn, Aik.phosphatase , LDH, Inorganic P, Total Protein; Cholesterol and Albumin were performed on a SMA 12 Technicon autoanalyzer; Hemoglobin, Hematocrit, mean erythrocyte volume, erythrocyte and total leukocyte numbers were performed by aid of a SMA 7 Technicon autoanalyzer. Platelet number were evaluated by aid of a Coulter Counter LTD ; protein and lipoprotein electrophoresis were performed on cellulose acetate strips and stained with Ponceau 5 and Sudan Schwartz B respectively leukocyte percentage was evaluated microscopically after Eosin-Methylene Blue staining . Blood glucose was estimated enzymatically with the aid of a Geansaec-Rotoleder apparatus : Cl" and Na+ concentrations were determined With a Chloride Meter electrode and a Unicam Atomic absorption apparatus respectively . SL 070421 2- In this report, the dat- of male and female animals were grouped since at preliminary analysis no significant differences were found between sexes . Statistical significance of the data (which are presented as means _+ one standard error) was evaluated by means of Duncan's multiple range test . Results obtained can be summarized as follows : 1. BUN : no statistically significant differences were observed either between control and treated animals nor between the animals differently treated with ECD (Table 1) . 2. Blood Glucose : a tendency towards a lowering in values was present in groups 1 and 3 ; considering that there is no clear dose-rcsponsivcncss in these changes, these modifications are of dubious biological significance (Table 2) 3. Bilirubin : no significant differences could be detected . 4. Serum total protein levels : statistically, the only significant differences was observed between Control and Group 3 (ECD 10 ppm) animals ; its biological significance as such is again of dubious importance 5. Serum albumin levels : a tendency towards an increase in albumin levels in the exposed animals in comparison to control was observed ; this tendency appeared moreover negatively correlated with the level of exposure . In fact Controls were significantly different from treated rats of Groups 2,3 and 4 but not of Group 1 ; in addition Group 1 was different from Groups 2,5 and 4 and the Group 2 from Groups 3 and 4, whereas no differences were present between Groups 3 and 4 in this parameter . SL 070422 3- 6. SGOT : no significant differences could be detected between control and ECD treated animals . 7. LDH : significant increases in serum LDH were observed in ECD exposed rats , changes being roughly dose related. The biological significance of these modifications remains to^be determined . 4 8. Cholesterol : Rats exposed to 5 and 10 ppm ECD appeared to show 1 _,wer levels than animals treated with 250 ppm . The biological significance of this difference appears to be dubious . 9. Uric acid : significant decreases in uric acid levels were detected in all groups of rats exposed to ECD . The magnitude of the effect was apparently inversely related to *the ECD dose . 10. Alkaline phosphatase : levels of this enzyme appeared to be significantly lower in animals exposed to 250 and 50 ppm than control . 11. Plasma 'chilomicrons : a significant, decrease in plasma levels was observed in animals exposed to 5 ppm . 12. Alfa-lipoproteins : statistically significant differences were onlyobserved among ECD treated grqtps and their biological significance is rather dubious . 13. Beta-lipoproteins : Significant reductions were observed in group 2 (50 ppm) and 4 (5 ppm), however the lack of a dose-response relationship casts some doubts on their significance . 14. Pre alfa lipoproteins : a significant increase was detected in groups exposed to 50, 10 and 5 ppm , without a clear dose response relationship . 15. Pre beta lipoproteins : No significant differences were found between controls and Group \ the differences being present only between Group 1 and 2, 1 and 4 and Groups 3 and 4 . SL 070423 4- 16. Beta/Alfa lipoprotein ratio : a signficant reduction was found in groups 2 and 4 as consequence of a lower beta lipoprotein level (see Tabic 13) . 17. Percent serum albumin : a significant roughly dose related increase in percent albumin was present. 18. Alfa 1 globulins : no apparent effects of ECD treatment were seen . 19. Alfa 2 globulins : all ECD treated groups showed alfa 2 globulins levels lower' than control. No dose-response effects could be identified . 20. Beta Globulins : increased beta globulins levels were found in rats exposed to the lowest ECD dose . 21. Gamma globulins : a borderline (p<0.05) reduction was observed only in group 1 (250 ppm) . 22. Na concentrations : a slight, although significant increase in Na levels was found in rats exposed to 250 ppm . 23. Ca concentrations : Ca levels were either increased (group 2) or decreased (group 4) . The significance of this data is questionable . 24. Cl" concentrations : no effects of ECD treatment . 25. Inorganic P : The decrease in P levels detected in group 3 is of dubious biological significance , 26. Leucocyte number : Leukocyte nunfcer appear to be increased following exposure to ECD, the effects being inversely related to the dose . 27. Hematocrit r Although a tendency toward as increase in HT was present in group 1, the difference with controls was not significant so that the only statistical difference was between Group 1 and 4 . SL 070424 5- 28, Haemoglobin concentration : modifications of haemoglobin levels are of borderline statistical significance (p<^0.05 and of doubtful biological meaning . 29 and 30 . Erythrocyte numbers and mean erythrocyte volume : no apparent effects of ECD treatment . 31. Platelet numbers : although the values were lowered in all treated groups in respect to controls, without a dose-response relat none of the differences were statistically significant. 34. Segmented neutrophilic leukocytes : The statistical difference found between groups 3 and 4 is of questionable biologic vai: 36. Lymphocytes : The slight although statistically significant difference between groups 2 and 4 is probably of restricted value . 32,33.35 and 37 : No significant effects of this ECD exposure on basophilic and eosinophilic leukocytes,monocytes and non segmented neutrophils was found . SL 070425 6- Comment: Although changes in sone parameters were observed in ECDexposed animals (e.g. LDii, uric acid, alk. phosphatase,etc.) the significance of these modifications from control values appears at this time difficult to assess considering tire frequent lack of dose-responsiveness in the changs observed and/or the discordant results in parameters evaluating the activity of the same organ . Accordingly no real coherent picture can yet be proposed. The data of the next testing period are thus needed to assess the significance, if any, 6f the modifications so far recorded. , SL 070426 Table 1 - BUN values in. control and BCD-exposed rats . CONTROL Rat No. mg GROUP Rat No. 1 mg" GROUP 2 Rat No. mg$ SOI 24 S03 18 SOS 19 SOS 24 sod 15 S10 18 S47 21 S43 18 S49 15 850 15 S51 15 853 16 X 18.17 S.E. 0.96 601 20'*' 602 15 603 18 604 26 605 21 606 17 603 19 646 21 647 17 648 18 649 17 650 16 651 15 652 16 653 15 X + S.E. . 18.07 0.77 401 402 - 403 404 405 406 407 4 OS 446 447 443 449 450 451 452 453 X _+ S.E. No statistically significant differences were found between any group 11 15 15 12 12 12 15 IS 22 21 18 20 13 18 21 19 16.05 0.90 GROUP 3 Rat No. mg % 201 202 203 204 205 206 207 20S 246 247 248 249 250 251 252 253 10 IS 18 21 23 19 14 21 21 ' 18 16 15 19 18 16 17 X 17.56 + S.E. 0.80 GROUP 4 Rat No. mg i .i 2 3 4 5 6 7 8 46 47 43 49 51 52 53 16 18 10 16 20 ' 21 21 22 13 12 16 13 14 IS 16 X 16.40 + S.E. 0.93 SL 070427 SL 070428 Table 2 - Serum Glucose in Control and ECD-exposed rats CONTROL Rat No. mg % GROUP 1 Rat No. mgl GROUP 2 Rat No. mgi GROUP 3 Rat No. mg* 803 152 804 106 SOS 150 807 108 803 141 6C9 86 810 120 847 122 843 158 849 91 850 83 851 125 852 100 S53 108 601 88 602 145 603 103 604 101 605 105 606 130 607 109 603 87 646 101 647 113 648 125 649 115 650 128 651 95 652 115 401 402 403 404 405 406 407 408 446 447 448 * 450 451 452 136 119 108 100 155 127 132 128 139 122 121 116 146 124 201 202 203 204 205 206 207 208 246 247 248 249 250 251 253 X +S. E. 118.86 6.64 . ' 11.12 3.95 126.55 3.61 Statistical analysis : Group 2 vs 3 : P<0.01 ; Group 1 ves 2 : P<0.05 137 116 103 88 145 105 110 136 108 105 91 74 128 75 91 107.56 5.29 , GROUP 4 Rat No. mg \ 1 123 2 145 3 104 4 105 5 133 6 115 7 107 8 110 46 108 47 124 48 107 49 no 51 112 52 120 l 116.07 2.96 SL 070429 Tr.ble 3 - Serum Bilirubin levels in Control and ECD-exposed rats CONTROL Rat No. mg % GROW Rat No. 1 mg!, GROUP 2 Rat No. mg % SOI 0.6 S03 0.6 805 0.3 803 0.6 809 0.6 810 1.8 847 0.9 848 0.9 849 1-2 850 0.9 851 853 0.9 X 0.82 +S.E. 0.12 601 0.6 602 0.9 603 1.5 604 0.9 605 1.7 606 0.9 608 0.9 646 0.9 647 1.1 648 0.6 649 0.6 650 0.6 651 0.9 652 0.6 653 0.9 0.91 o.os 401 1.2 402 0.7 403 0.6 404 1.2 405 0.4 406 0.6 407 0.5 403 0.9 446 0.7 447 0.6 448 0.7 ' 449 1.2 450 1.5 451 0.6 452 0.7 455 0.9 * 0.81 0.03 No statistical significant differences were found between the groups . GROUT 3 Rat No. mg % 201 .0.5 202 0.6 203 0.4 204 0.3 205 0.4 206 0.3 207 0.6 208 0.6 246 0.6 247 0.6 248 0.3 249 0.6 250 1.2 251 0.7 252 l.S 253 i.5 0.72 0.11 GROUP 4 Rat No . mg % . 1 0.8 2 0.4 3 0.4 4 0.5 S 0.9 6 1.6 7 1.5 8 1.6 46 0.3 47 0.5 43 0.7 49 0.7 51 2.0 52 1.2 55 . 0.7 0.92 0.14 vV SL 070430 Table 4 - Serum total protein levels in Control and BCD-cxposcd rats . CONTROL Rat No. g * GROUP Rat No. 1' g* . GROUP 2 Rat No. g* 801 8.5 803 6.4 805 6.6 803 7.2 809 6.9 810 7.5 S47 6.6 S4S 6.6 849 7.8 350 7.6 851 8.7 853 6.3 x +S. E * 7.23 0.23 601 7.8 602 6.9 603 8.0 604 8.0 605 S.4 606 7.2 60S 7.5 646 6.9 647 7.2 648 7.0 649 6.5 650 6.4 651 7.0 652 6.6 653 7.0 7.23 +0.15 401 402 403 404 405 406 407 403 446 447 443 . 449 450 451 452 453 7.6 7.2 7.5 7.8 7.0 0.3 7.5 7.2 7.0 6.6 7.5 6.9 8.1 6.6 6.6 6.6 7.13 +0.13 Statistical analysis : group 3 vs C : p^O.OS GROUP 3 Rat No. O <9 \ 201 5.7 202 7.1 203 6.9 204 7.3 205 7.2 206 0.3 207 6.6 208 7.9 246 6.5 247 6.4 248 6.6 249 6.6 250 6.4 251 6.5 252 7.3 253 6.4 6.77 + 0.13 GROUP 4 * Rat No. g % 1 7.4 2 7.0 3 6.3 4 6.3 5 7.2 6 8.1 7 7.7 8 7.6 46 6.7 47 6.0 48 6.5 49 7.1 51 6.9 52 6.5 53 6.7 6.93 +_ 0.13 Tsble 5 - Serum albumin levels in Control and ECD-exposed rats . I CONTROL Rat No. 2 *4 GROUP Rat No. 1 g\ GROUP 2 Rat No. g \ I GROUP 3 Rat No. g % GRCUP 4 Rat No. g % SOI 3.3 S03 3.2 803 3.0 SOS 3.3 $09 3.3 810 3.6 S47 3.0 848 2.8 S49 1.8 850 2.9 851 3.7 853 3.0 601 602 603 604 605 606 ' 608 646 647 648 649 650 651 652 653 4.8 3.1 4.1 3.6 3.9 3.7 3.5 3.0 3.3 2.9 2.9 3.1 3.0 2.9 3.0 401 3.7 402 3.4 403 3.4 404 3.8 405 3.2 406 3.0 407 3.5 408. ` 3.3 446 2.9 447 3.0 448 7.5 n 449 6.9 450 6.9 451 ' 452 6.6 6.7 453 6.5' 201 6.7 202 7.6 203 7.4 204 6.9 20S 7.3 206 7.2 207 7.4 208 7.7 246 6.9 247 6.7 248 6.7 249 6.6 250 6.7 251 6.7 252 7.2 253 6.9 1 7.5 ? 7.4 3 7.3 4 7.2 5 7.3 6 7.9 7 7.7 8 7.6 46 5.6 47 6.3 48 6.8 49 5.9 51 6.8 52 6.9 53 7.9 X + S.E. 3.03 0.14 . 3.33 +0.12 4.37 + 0.51 7.04 + 0.09 7.07 + 0.18 Statistical analysis : Group C vs 4 : P <0.001 ; Group 1 vs 4 : P<0.001 ; Group 2 vs4 : p<0.001 ; Groups C Vs. 3 : PC0.001 Group 1 vs 5 : P<0.001 ; Group 2 vs 3 : p<0.001 ; Group C vs 2 : p<0.005 ; Group 1 vs 2 : P<0.005 . SL 070431 Table 6 - Serum SGOT levels in Control and ECD-exposed rats . < --COXTEOI Rat No. rrU/inl GROUP 1 Rat No. iiiJ/ml GROUP 2 Rat No. irU/ml GROUP 3 Rat No. mU/ml 801 803 805 SOS S09 810 847 818 S49 S50 S51 853 |l- ' X + S.E. 78 601 131 401 . 171 105 602 159 402 99 84 603 168 403 87 102 604 135 404 150 129 605 195 405 100 216 606 132 406 132 97 60S 107 407 100 93 646 129 403 102 96 647 171 416 126 144 643 129 447 135 153 649 135 448 117 * 96 650 126 449 106 651 204 450 108 652 115 451 116 653 136 452 141 455 156 116.08 11.28 144.80 7.32 121.63 6.00 No statistically significant differences, were found between the groups . ^ 201 96 202 159 203 132 204 107 205 100 206 111 207 117 208 126 246 159 247 103 248 147 249 114 250 156 251 144 252 216 253 207 137.44 8.93 GRCUP 4 Rat No r.U/ml . 1 126 2 90 3 118 4 92 5 141 6 264 7 180 8 237 46 102 4 7 106 48 108 49 ICS 51 150 52 159 S3 113 159.6 13.41 SL 070432 Table 7 - Scrum LDH levels in Control and ECD-cxposed rats . CONTROL Rat Ko. nU/ml GROUP Rat No. 1 mU/ml GROUP 2 Rat No. mU/ml GROUP 3 Rat No. inU/ml GRCUP 4 Rat No.mU/::il 801 803 805 808 809 810 847 843 349 850 85l` 853 300 720 . " 280 420 600 930 1050 900 . 930 960 1270 900 601 602 603 604 605 606 60S 646 647 648 649 650 651 652 653 ` 1290 1350 1380 1050 1440 1110 810 1080 1440 1560 1590 1380 1490 1380 1320 ' 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453' 1230 660 570 1200 990 500 650 660 1290 1380 1620 * 1330 1020 1440 1310 162 201 900 202 1200 203 1200 204 840 205 870 206 750 207 1020 208 1320 246 1500 247 1110 248 1290 249 1300 250 1140 251 1350 252 1560 253 1150 1 + S.E. 771.67 89.80 1311.33 54.96 1091.87 94.49 1160.00 58.81 Statistical analysis : Group C 1 : P< 0.001 ; Group 4 vs 1 : P<0.001 ; Group 2 vs 1 : P-CO.005 ; Group C vs 3 : P <0.005 ; Group 3 vs 4 : P<0.05 ; Group 3 Vs 2 : P<0.05 1 1020 2 300 3 590 4 620 5 690 6 1230 7 1140 8 1320 46 670 47 1000 48 1060 49 760 51 1110 52 1110 S3 790 8 94.a 73.8 SL 070433 TabIff 8 Seruin cholesterol levels in Control and ECD-exposed rats CONTROL at No. mg % GROUP 1 Rat No. mg % . GRCUP 2 Rat No. mg \ 801 S02 803 804 SOS 807 803 S09 810 S47 S43 S49 850 8S1 8S2 853 165 105 ' 75 - 105 - 105 105 105 90 120 90 115 150 - 10S * 601 602 603 604 605 606 ' 607 608 646 647 643 649 650 651 * 652 653 X S.E. 110.38 4.58 w Statistical analysis: group 3 vs 1: p 120 401 105 402 145 403 150 404 ' ISO 405 120 406 - 407 130 408 110 446 135 447 ns 448 95 449 105 450 105 451 105 452 125 453 121.00 2.54 0.00S; group 4 vs 1: p 125 120 105 120 120 110 no no 105 so no 120 130 115 125 125 115.00 2.92 0.0S GROUP 3 Rat No. mg 1* 201 202 203 204 205 206 207 20S 246 247 249 250 251 252 253 V no no 95 90 105 90 120 120 no 105 90 80 90 105 * 115 105 102.61 4.21 GROUP 4 Rat No. . mg % 1 90 2 100 3 100 4 90 5 no 6 120 7 no 8 no 46 90 47 90 43 90 49 90 SI 120 52 135 S3 135 1C5.33 CO * 070434 Table 9 - Uric acid serum levels in Control and ECD-exposed rats CONTROL Rat X'o. mg % GROUP 1 Rat No. mg % GRGJP 2 Rat No. mg % GROUP 3 Rat No. mg % GROUP 4 Rat No. mg % 801 802 803 804 .805 807 808 809 810 S47 848 849 850 851 852 853 ' 10.2 - 3.6 - 5.7 - 4.8 4.2 . 4.2 3.9 4.5 3.9 3.9 4.6 -3.6 601 602 603 604 60S * 606 ' 607 60S 646 647 648 649 650 651 652 653 3.9 3.8 3.6 3.6 3.S 3.3 - 3.1 3.0 3.6 3.4 3.0 2.4 2.4 2.4 2.1 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453 2.4 2.8 2.9 2.7 2.7 2.5 2.6 2.7 2.7 2.7 2.8 2.8 2.7 2.5 2.7 2.9 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 2.7 2.7 2.6 2.4 2.6 2.4 2.4 2.5 2.7 2.9 2.7 2.7 2.9 3.0 2.8 2.9 1 3.0 2' 2.8 3 2.7 4 2.7 5 2.7 6 3.1 7 3.3 8 3.4 46 3.1 47 3.0 43 2.7 49 2.7 51 3.0 52 3.0 55 3.0 * X S.E. 4.76 0.52 3.14 0.15 Statistical analysis: group C vs 3: p 0.001; group C vs 2: p 2.69 0.03 2.68 0.05 0.001; group C vs 4: p ^0.001; group C vs 1: p 2.99 0.09 0.001. ` SL 070435 VS^hobo 10 - Scrum levels of alkaline phosphatase in Control and ECD-cxposcd rats . CONTROL Rat No. niJ/ml 801 SC2 803 804 805 SO? SOS 809 310 847 848 849 850 351 S52 853 150 - 144 - 150 - 96 84 84 180 108 160 246 174 - 130 GROUP 1 Rat No. mU/ml 601 602 603 604 605 606 * 607 60S 646 647 648 649 650 6S1 652 653 78 90 57 60 69 57 - 55 74 120 71 75 130 138 123 102 GROUP 2 Rat No. iriU/ml 401 402 . 403 404 405 406 407 408 446 447 448 449 450 451 452 453 122 156 127 84 66 75 102 60 ISO 144 120 120 102 129 117 114 GROUP 3 Rat No. nU/ml 201 202 203 204 205 206 207 208 246 247 n 248 249 250 251 252 253 68 169 66 79 74 64 132 63 180 117 120 128 135 162 210 138 X * S.E. 142.17 3.45 86.60 ' 7.43 119.19 7.10 119.06 11.60 Statistical analysis : Group C vs 1 : P<0.001 *. Group C vs 2 : P<0.05 GROUP 4 Rat No.. m'J/iiil 1 103 2 64 3 72 4 62 5 102 6 75 7 120 8 126 46 28 47 135 48 124 49 1S1 SI 186 52 126 53 119 111.87 9.87 Table 11 - Plasma chilcmicrons in Control and CCD-exposed.rats SL CONTROL Rat No. mg % GROUP 1 Rat No. mg % GROUP 2 Rat No. mg$ GROUP 3 Rat No. mg % GROUP 4 Rat No.. mg % p SOI 802 O 803 2 804 805 bt> 807 80S S09 810 847 848 849 850 851 S52 853 X +S.E. 6.8 10.5 8.9 6.0 9.0 5.0 4.9 12.5 8.4 11.6 10.6 9.7 8.1 8.0 5.2 601 602 603 604 605 606 '607 60S 646 647 648 649 650 651 652 653 13.4 10.2 9.7 8.3 9.5 8.9 9.9 5.2 4.2 9.8 9.1 8.5 4.6 8.7 6.9 9.4 401 8.8 402 8.1 403 9.3 404 9.0 405 8.1. 406 7.0 407 9.8 403 10.0 446 7.7 447. 7.0 448 7.0 449 . 8.1 4 SO 9.9 451 452 9.2 7.9 453 . 8.0 201 202 205 204 205 206 207 203 246 247 * 248 249 250 251 252 253 8.0 10.0 9.1 12.1 8.0 9.5 S .9 ,8.8 8.0 6.5 6.3 5.0 8.1 9.0 7.4 4.4 1 2 3 4 5 6 7 8 46 47 4S 49 51 52 53 8.41 0.60 8.52 0.58 8.43 0.26 8.06 0.47 Statistical analysis : Group 1 vs 4 : P^O.05 ; Group C vs 4 : P^0.005 ; Group 2 vs 4 : P<0,05 . 5.0 6.2 4.0 8.6 7.0 4.7 6.7 9.0 S .5 5.0 8.9 8.1 5.2 6.7 10.0 6.91 0.48 Table 12 - Percent concentrations of alpha-lipoproteins in Control and ECD-exposed rats . 4 CONTROL Rat No* mg % .GROUP 1 Rat No. mg % GRCUP 2 Rat No. mg % GRCUP 3 Rat No. mg % SOI 802 803 804 805 307 SOS S09 810 847 843 349 850 S51 852 853 23.5 15.4 15.9 16.5 20.5 22.1 25.6 23.0 27.0 17.3 21.6 10.0 17.7 13.8 18.2 601 602 603 604 605 606 ' 607 608 646 647 648 649 650 651 652 653 12.1 12.7 16.0 18.6 15.5 15.9 15.8 16.5 24.5 28.1, 23.5 25.5 12.6 11.1 12.0 9.0 401 29.5 402 22.5 403 17.8 404 18.3 405 24.5 406 25.3 407 20.4 408 19.3 446 26.3 447 448 449 450 451 452 453 21.1 21.0 20.7 18.7 19.8 22.5 23.1 201 202 205 204 205 206 207 20S 246 247 * 248 249 250 251 252 253 15.0 16.9 14.8 20.2 18.1 22.6 16.2 17.8 16.9 17.0 14.7 16.1 20.7 22.1 20.3 19.1 X + S.E. 19.41 1.35 16.84 1.43 21.93 0.80 18.03 0.64 Statistical analysis : Group 1 vs 4 : P<0.001 ; Group 3 vs 4 : P-^0.01 ; Group 1 vs 2 : P-<0.01 Group 2 vs 3 : P<0.05 GROUP 4 Rat No. mg i 1 20.9 2 20.9 3 27.5 4 17.9 S 22.4 6 24.7 7 25.5 3 26.0 46 20.8 47 25.2 48 20.6 49 21.9 51 18.5 52 18.7 53 21.9 22.41 0.78 SL 070436 SL 070437 Table 13 Percent concentrations of Beta-Lipoproteins in Control and ECD-exposed rats CONTROL Rat No. mg % GROUP 1 Rat No. mg GROUP 2 Rat No. mg s GROUP 3 Rat No. mg * SOI 37.6 802 49.8 603 40.5 804 49.7 805 36.4 807 38.1 SOS 52.1 S09 34.8 810 33.2 847 40.6 843 38.5 849 36.7 850 . 41.8 851 31.2 852 853 45.9 601 602 605 604 605 606 ' 607 60S 646 647 648 649 650 651 652 653 42.4 41.7 45.7 42.2 42.3 41.9 34 .3 35.9 27.1 31.0 32.4 26.8 48.6 41.0 44.0 50.6 401 402 . 403 404 405 406 407 408 446 447 448 449 450 451 452 453 18.9 33.3 41.4 42.4 30.2 32.0 31.9 32.5 31.4 38.9 31.5 34.5 37.9 30.8 30.0 30.2 201 39.5 202 39.3 203- 38.0 204 35.4 205 34.6 206 33.9 207 36.5 208 38.5 246 39.6 247 36.6 248 38.4 249 39.9 250 37.8 251 29.3 252 36.1 253 36.2 X + S.E. 39.46 1.43 39.23 1.80 32.95 1.40 36.85 0.68 Statistical analysis : Group C vs 2 : P<0.05 ; Group C vs 4 : P<O.OS ; Group 1 vs 2 : P< O.OOS ; Group 1 vs 4:P <0.01 ; Group 2 vs 3 : P<0.05 . . X r *' GROUP 4 Rat No. mg * 1 33.3 2' 33.7 5 33.0 4 43.0 5 36.5 6 38.47 28.8 8 31.1 46 36.0 47 54.1 48 54.5 49 28.9 51 57.0 52 29.9 55 28.3 33.77 1.05 Table 14 - Percent concentrations of pre-alpha-lipoproteins in Control and ECD-exposed rats CONTROL Rat No. mg % GROUP 1 Rfit No. mg % GROUP 2 Rat No. mg* GROUP 3 Rat No. mg * GROUP 4 Rat No. mg ^ 801 5.0 602 4.7 803 6.2 804 6.4 805 5.9 807 9.7 808 7.6 809 7.9 810 6.2 847 7.7 848 3.2 849 5.0 850 5.1 851 6.1 852 853 9.9 601 602 603 604 605 606 ' 607 608 646 647 648 649 650 ` 651 652 653 7.2 5.7 5.4 4.7 4.5 7.6 5.7 6.8 7.6 6.7 8.5 12.1 8.3 '9.7 9.4 5.7 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453 13.8 13.2 6.7 7.9 10.5 12.0 10.8 11.9 11.6 13.4 11.0 10.4 8.6 12.9 11.5 19.9 201 8.4 202 8.1 203 10.7 204 7.3 205 10.2 206 8.6 207 8.8 203 8.8 246 9.3 247 9.7 ^ 248 7.4 249 8.4 250 10.9 251 13.7 252 13.5 253 9.2 1 12.7 2 15.4 3 11.6 4 10.1 5 9.7 6 11.3 / '1.3 8 11.2 46 14.3 47 10.5 48 11.5 49 13.5 51 1.3 52 12.7 S3 11.5 X 6.44 7.22 11.61 9.56 12.22 +S.E. 0.48 0.51 0.74 0.47 0.43 Statistical analysis : Group C vs 4 : P<0.01 ; Group 1 vs 4 : P<0.01 ; Group 3 vs^4 : P<0.05 ; Group C vs 2 : P-(0.01 ; Group 2 vs 1 : P <0.001 ; Group 2 vs 3 : P <0.05 ; Group 1 vs 5 : P <0.05 ;Group C vs 5 : P< 0.001 , SL 070438 Table V 15 - Concentrations of prebeta lipoproteins in Control and ECD-exposed rats . i CONTROL Rat Mo. mg " GROUP 1 Rat No. mg % GROUP 2 Rat No. mg % GROUP 3 Rat No. mg % 801 802 803 S04 805 807 SOS S09 810 847 843 849 850 851 S52 S53 ' . 26.9 19.6 29.5 21.2 23.1 25.3 30.1 16.7 20.2 24.9 26.5 39.1 27.6 40.5 20.3 601 602 603 604 605 606 ' 607 60S 646 647 648 649 650 651 652 653 24.9 29.5 23.2 26.2 29.2 25.6 35.1 36.5 36.5 2473, 26.4 26.9 25.9 29.3 28.5 26.2 401 29.1 402 23.1 403 24 .3 404 22.1 405 27.0 406 24.2 407 408 446 447 448 449 450 451 452 453 26.8 26.3 23.9 27.0 29.7 26.4 24.6 26.0 28.8 29.3 201 202 203 204 205 206 207 208 246 247 n 248 249 250 251 252 253 29.4 27.3 28.7 26.9 29.4 25.5 30.3 27.2 26.3 30.2 33.2 30.9 24.8 26.S 22.9 32.0 + S.E. 26.42 1.74 28.39 1.05 Statistcal analysis : Group 1 vs 4 P<0.01 26.16 0.58 28.22 0.69 ; Group 4 vs 3 : P<0.05 ; Group 1 vs 2 : P<0.05 GROUP 4 Rat No. . mg % 1 28.1 Z 26.7 3 24.5 4 20.4 5 24.6 6 20.9 7 24.8 8 23.1 46 20.7 47 25.4 48 24.3 49 27.6 51 28.5 52 26.8 53 25.3 24.78 0.67 SL 070439 Table 16 - Lipoproteins ration in Control and ECD-exposed rats. CONTROL at No. ratio GROUP 1 Rat No. ratio GROUP 2 Rat No. ratio GROUP 3 Rat No. ratio GROUP 4 Rat No.. ratio 801 1.6 802 3.2 805 2.9 804 3.0 S05 1.7 807 1.7 808 1.2 809 1.2 310 1.4 847 2.3 8J8 1.7 849 3.6 850 2.3 851 2.2 S52 -* 853 2.5 601 602 603 604 605 606 ' 607 608 646 647 648 649 650 651 652 653 3.5 3.2 2.8 2.2 2.7 2.6 2.1 2.1 1.1 1.1 1.3 1.0 3.8 3.6 3.6 5.6 401 402 . 403 404 405 405 407 408 446 447 443 449 450 451 452 453 0.6 1.4 2.3 2.3 1.2 1.2 * 1.5 1.6 1.1 1.8 1.5 1.6 2.0 1.5 1.3 1.3" 201 2.6 202 2.3 203 2.5 204 1.7 205 1.9 206 1.5 207 2.2 208 2.1 246 2.3 247 2.1 , 24S 2.6 249 ' ' 2.4 250 1.8 251 1.5 252 1.1 253 1.8 1b 2 1.6 3 1.2 4 2.4 5 1.6 6 1.5 7 1.1 8 1.2 46 1.7 47 1.3 48 1.6 49 1.3 51 2.0 52 1.6 S3 1.1 I S.fi. 2.17 . 0.19 2.64 0.31 1.51 0.11 ^ Statistical analysis: group 1 vs 4: p 0*001; group 3 vs 1: p *0.01; gtoup C vs 2: p SoJ ios is 2.01 0.11 0*01; group C vs 4: p 0.01 1.52 0.09 Table 17 Percent serum albumin in Control and ECD-exposed rats. CONTROL Rat No. \ GROUP 1 Rat No. % GROUP 2 Rat No. % GROUP 3 Rat No. % GROUP 4 Rat No.. % 801 802 803 S04 SOS 807 808 809 810 S47 848 849 S50 851 852 853 35.3 41. S 43.8 41.5 47.9 43.2 47.2 48.8 48.3 40.9 39.8 36.6 40.6 30.8 42.7 601 602 603 604 605 606 ' 607 608 646 647 648 649 650 651 652 653 48.5 42.9 47.6 49.5 45.0 52.1 44.0 44.3 46.9 45.6 43.3 44.4 44.1 44.3 48.8 48.8 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453 52.5 ' 55.7 52.3 52.2 44.3 S2.3 50.4 46.2 45.3 50.2 42.5 43.5 40.2 41.6 45.1 40.6 201 202 203 204 205 206 207 208 246 247 * 248 249 2S0 251 252 253 50.5 47.6 48.7 44.7 49.1 45.6 46.5 45.5 42.2 44.2 45.1 45.5 42.7 45.0 ' 42.2 43.8 1 2 3 4. 5 6 7 8 46 47 43 49 51 52 53 i X S.E. 40.48 2.93 46.26 0.67 47.18 1.25 45.. 56 0.61 Statistical analysis: group C vs 2: p t/3 r o o> S' 0.005; group 2 vs 4: p 0.01; group C vs 1: p *0.01; group C vs 3: p ... 0.05 50.5 50.7 48.1 59.4 37.3 43.8 42.9 39.6 25.3 48.7 44.0 29.6 41.8 41.9 42.3 42.59 1.91 Table 18-Scrum alfa globulins content in Control and CCD-exposed rats CONTROL Rat No. i GROUP 1 Rat No. % GROUP 2 vRat No. SOI S02 803 804 80S S07 SOS S09 810 S47 843 845 SSO 8S1 SS2 853 X ' S.E. 15.1 15.7 20.2 16.7 18.2 14.6 13.0 12.5 13.9 22.0 22.7 18.2 19.4 14.4 - 18.8 1. 17.03 0.83 601 602 603 604 605 606 1 607 608 646 647 648 649 650 651 652 653 19.1 19.0 17.2 16.0 16.0 17.2 16.5 17.8 14.5 16.8 17.7 19.4 17.5 18.3 IB.3 17.8 17.44 0.32 401 402 403 404 405 406 407 408 446 447 44S 449 450 451 452 453 17.3 12.1 17.6 17.0 14.4 12.7 13.2 14.4 16.2 13.2 20.4 16.8 13.4 20.3 16.6 17.4 15.81 0.64 " No statistically significant differences were found between the groups, o'osi JS GROUP 3 Rat No. l 201 202 203 204 205 206 207 208 246 247 , 248 249 250 251 252. 253 ` 15.7 17.5 18.8 15.3 14.9 16.2 16.3 15.4 20.3 19.2 16.2 13.5 11.4 16.0 15.5 14.4 16.04 0.55 GROUP 4 Rat No. % 1 14.1 2` 15.1 3 14.9 4 12.9 5 18.8 6 17.1 7 17.4 8 14.7 46 28.1 47 19.0 43 19.4 49 14.2 51 14.8 52 22.7 53 20.0 17.41 1.06 Table 19 - Serum alfa 2 globerlins content in Control and ECD-exposed rats 4 CONTROL Rat No. .% GROUP 1 Rat No. X GROUP 2 Rat No. % GROUP 3 Rat No. % 801 802 03 804 SOS 807 SOS 809 810 S47 848 S49 850 , 851 852 853 11.5 14.2 12.7 18.2 11.6 15.5 11.0 12.3 15.8 12.0 13.9 16.2 11.7 13.3 601 602 603 604 605 606 ' 607 608 646 647 648 649 650 651 652 653 9.8 12.0 12.3 8.6 14.0 11.7 15.2 13.1 11.8 12.4 14.8 10.8 9.2 12.9 10.7 10.7 401 402 403 404 405 406 407 40S 446 447 448 449 450 451 452 453 10.8 9.8 7.8 9.2 9.2 9.6 10.1 11.3 11.7 12.9 10.7 11.5 14.1 10.7 11.1 15.3 201 202 203 204 205 206 207 203 246 247 * 248 249 250 251 252 253 9.9 9.0 9.7 8.5 9.4 9.6 12.2 10.4 10.7 11.0 12.9 10.6 17.3 14.0 10.3 13.9 X s.e. 13.56 . 0.58 " Statistical analysis: group C 2: p 11.88 0.47 0.005; group C 3: p 10.99 0.47 11.21 0.58 0.01; C 4: p 0.01; C 1: p 0.05 GROUP 4 Rat No.. % 1 9.1 2` 9.3 3 8.9 4 11.1 5 12.6 6 13.0 7 12.6 8 13.4 46 15.4 47 11.1 43 ' 8.8 49 10.5 51 15.6 52 10.6 53 8.8 11.36 0.60 070443 Table 20 - Scrum beta globulins content in Control and ECD-exposed rats. 4 CONTROL lat No. % GROUP 1 Rat No. i GROUP 2 Rat No. t GROUP 3 Rat No. % GROUP 4 Rat No. \ 801 15.6 802 17.8 ' 303 IS.5 804 12.8 605 13.0 807 15.9 6C3 15.9 SOD 13.8 810 13.4 847 17.7 843 16.1 849 16.5 850 15.1 851 10.5 852 853 13.3 601 602 603 604 605 606 * 607 60S 646 647 64 S 649 650 651 652 653 13.7 14.2 12.5 14.7 14.0 11.9 15.0 14.3 16.2 14.8 15.7 17.3 19.0 . 16.3 16.7 15.2 ' 401 402 403 404 405 406 407 408 446 447 44S 449 450 451 452 453 10.6 12.2 10.9 12.5 17.8 15.9 15.0 16.7 18.1 14.8 17.1 18.6 18.9 19.5 17.8 17.5 201 202 203 204 205 206 207 208 246 247 ^ 248 249 250 251 252 253 12.0 13.7 13.6 15.0 14.5 16.2 15.1 16.4 17.4 18.4 18.3 15.5 17.8 14.0 22.5 17.2 1 17.0 2 15.1 3 19.5 4 15.5 5 17.6 6 16.1 7 15.8 8 20.0 46 23.7 47 20.5 48 19.3 49 22.2 51 17.7 52 18.2 53 19.6 T S.E. 14.86 0.52 15.09 0.45 tatistical analysis: group C 4: p 0.001; group 1 4: p 15.87 0.73 0.001; group 2 4: p 16.10 0.63 0.005; group 3 4: p 0.01 13.48 0.65 SL 070444 Table 21 - Serum gamma globulins in Control and ECD-exposed rats < CONTROL RcIl No. % GROUP 1 Rat No. \ 801 802 803 804 805 S07 803 809 810 S47 848 349 850 851 852 853 22.5 10.9 9.1 10.5 10.0 -10.7 12.9 12.5 11.4 7.5 7.9 11.8 13.3 11.9 - 12.2 601 602 603 604 605 606 ' 607 60S 646 647 648 649 650 651 652 653 9.4 11.7 10.1 11.4 11.1 7.5 10.0 10.7 10.4 10.5 9.1 8.7 10.4 9.0 6.0 8.4 X S.E. 11.67 0.89 Statistical anctysis: group c 1: p 9.65 0.38 0.05 GROUP 2 Rat No. % 401 402 403 404 405 406 407 408 446 447 448 449 450 451 - 452 453 8.8 10.2 11.6 ` 9.6 14.2 10.2 10.9 10.6 9.3 8.8 12.0 9.4 14.2 10.8 10.5 9.8 10.68 0.41 GROUP 3 Rat No. \ 201 202 203 204 205 206 207 208 246 247 ^ 243 249 250 251 252 253 11.8 12.6 9.8 17.1 12.2 13.1 11.4 12.0 9.5 7.3 8.0 15.7 11.2 11.1 * 10.5 10.9 11.51 0.62 GROUP 4 Rat No. % 1 9.1 2 11.4 3 10.1 4 10.4 5 15.0 6 10.7 7 11.3 8 15.3 46 7.5 47 11.0 43 10.0 49 51 10.4 52 8.3 55 9.4 10.56 0.51 SL 070445 Table 22 - Na+ concentrations in Control and ECD-exposed rats CONTROL Rat No. mEq/ml GROUP 1 Rat No. mEq/ml 801 802 803 804 805 807 808 S09 810 S47 848 849 850 851 852 853 ' 132 144 150 139 141 141 139 137. 139 141 141 147 147 147 ' 144 601 602 603 604 605 606 607 608 646 647 648 649 650 651 652 653 147 150 144 141 144 144 144 147 147 150 141 144 147 147' 141 144 X S.E. 141.93 1.20 145.13 0.72 r Statistical analysis: group C 1: p 0.01 GROUP 2 Rat No. mEq/ml 401 402 403 404 405 406 407 408 446 447 448 449 450 451 4S2 453 144 129 150 147 139 141 144 144 139 147 141 141 139 141 ' 147 144 142.31 1.21 GROUP 3 Rat No. mEq/ml 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 144 144 141 141 139 141 144 147 150 141 139 137 134 144 150 147 142.69 . 1.11 GROUP 4 Rat No. mEq/ml 1 141 2` 144 3 139 4 141 5 144 6 139 7 141 8 147 46 144 47 141 48 144 . 49 147 51 144 52 141 53 137 142.27 0.75 Tabic 23 - -Ca++-concentrations in Control and ECD-exposed rats CONTROL Rat No. JiiEq/rcil GROUP 1 Rat No. mEq/ml GROUP 2 Rat No. mEq/ml GROUP 3 Rat No. mEq/ml GROUP 4 Rat No.. jnEq/rrl 801 10.5 802 805 7.8 804 SOS 9.0 807 SOS 8.7 S09 8.7 810 7.8 847 8.4 848 8.4 840 9.0 8 SO 9.0 SSI 7.0 852 8S3 7.8 601 602 603 604 605 606 ' 607 608 646 647 64 S 649 650 651 652 653 8.4 8.1 9.2 8.5 8.9 8.9 - 8.9 8.5 8.5 8.4 8.6 8.4 8.7 8.7 9.2 401 402 403 404 405 406 407 40S 446 447 448 449 450 451 452 453 9.3 9.0 9.3 9.9 9.3 8.4 9.3 9.0 9.4 9.3 9.5 9.0 9.1 9.0 8.7 8.7 201 202 203 204 205 206 207 208 246 247 ^ 248 249 250 251 252 253 6.3 8.5 8.1 8.7 8.1 7.5 8.7 9.1 8.4 8.3 8.S 7.6 7.2 8.1 8.2 7.5 1 8.4 ' 2 8.3 3 7-1 4 7.1 5 8.3 6 8.2 7 8.1 8 8.1 46 S.9 47 7.5 48 7.S 49 7.8 51 7.5 52 7.S S3 7.8 S.E. l 8.51 0.25 8.66 0.08 9.13 0.09 * 8.05 0.17 7.91 0.15 Statistical analysis: group 1 3: p 0.005 ; group C v.s. 4: p 0.01; group 1 v.s. 4: p *i).001; group 2 v.s. 3: p 0.001; group 2 v.s. 4: p 0.001; group C v.s. 2: p 0.01; group 1 v.s. 2: p 0.01 ' SL 070447 O CO SL 070448 Table 24 - Cl~ concentrations in Control and CCD-exposed rats CONTROL (at No. mEq/ml GROUP 1 Rat No. mEq/ml GROUP 2 Rat No. mliq/ml 801 802 83.1 SC4 807 808 S09 310 S47 3-1S 849 S50 851 S52 853 100 102 103 107 102 108 105 100 106 104 105 107 101 104 98 601 602 603 604 605 606 607 608 646 647 648 649 650 651 652 653 101 106 107 104 105 104 107 102 102 103 105 101 101 102 100 101 401 402 403 404 405 406 407 408 446 447 448 449 4 SO 451 452 453 105 104 102 104 103 102 102 105 103 104 ' 103 105 105 102 ' 104 104 X' s.e. 103.47 0.76 103.18 0.57 103.56 0.29 No statistically significant differences were found between the groups. GROUP 3 Rat No. mEq/wl 201 . 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 101 104 102 103 108 1C5 104 103 105 102 104 102 106 103 105 105 105.87 0.45 GROUP 4 Rat No.. mJiq/r.'.l 1 1C6 2 105 3 104 4 105 5 1C2 6 106 7 106 8 107 46 102 47 1C4 43 1C5 49 103 51 106 52 102 53 104 104.37 0.41 Table 25 - Inorganic P concentrations in Control and ECD-cxposed rats CONTROL Rat No. mEq/ml GROUP 1 Rat No. mEq/ml GROUP 2 Rat No. mEq/ml GROUP 5 Rat No. mEq/nl GROUP 4 Rat No.. mEq/nl S01 S02 SOS 804 SC5 S07 SOS S09 810 S47 848 849 850 ; S51 S52 853 9.9 - 8.1 - 8.1 - 7.2 8.1 . 9.3 7.8 7.5 11.4 10.5 9.4 7.8 601 9.6 602 9.0 603 9.1 604 9.3 605 9.9 606 8.7 607 60S 8.3 646 9.5 647 12.5 643 8.4 649 9.6 650 9.6 651 9.6 652 8.1 653 9.3 401 402 403 404 405 406 407 408 446 447 44$ 449 450 451 452 453 9.0 9.3 8.9 9.9 9.0 8.0 7.8 8.9 9.6 10.6 9.0 9.1 13.5 9.1 10.7 9.0 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 5.9 7.5 8.1 7.6 6.8 7.0 9.3 7.5 8.0 7.6 8.8 7.6 3.6 6.9 7.2 9'. 1 1 5.7 Z 6.9 3 6.9 4 5.8 5 8.4 6 9.7 7 9.0 8 9.7 46 11.1 47 8.4 48 8.1 49 7.2 51 9.3 52 8.4 S3 7.1 T. w S.E. 8.76 0.38 9.37 0.26 9.46 0.33 7.71 0.22 8.11 0.39 Statistical analysis: group C v.s. 3: p . 0.01; group 4 v.s. 1: p 0.01; group 3 v.s. 2: p 0.C01; group 2 v.s. 4: p O.COS group 1 v.s. 3: p 0.001 SL 0704 Table 26 - Leukocyte numbers in Control and ECD-cxposed rats CONTROL vat No. (x 103) GROUP 1 Rat No. (x 103) GROUP 2 Rat No. (X 103) GROUP 3 Rat No. (x 103) GROUP 4 Rat No.. (x 103) 801 8.0 802 5.2 803 7.8 S04 4.4 805 9.6 807 5.2 808 7.2 809 5.6 810 6.4 847 13.4 348 12.0 849 9.6 850 9.8 S51 9.4 852 12.6 853 8.8 601 602 603 604 60S 606 ` 607 608 646 647 648 649 650 651 652 653 6.4 S.2 6.0 8.0 13.2 9.0 6.8 9.6 10.0 8.0 7.4 12.4 12.0 11.6 13.6 7.4 401 402 . 403 404 405 406 407 408 446 447 448 449 450 451 452 453 9.2 21.2 10.0 10.4 9.0 12.0 5.8 10.8 15.6 21.8 17.2 14.0 7.6 20.4 12.6 21.6 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 17.2 14.2 10.0 22.0 12.0 14.6 13.4 12.6 21.0 15.4 14.8 17.0 13.0 17.4 17.8 12.8 1 8.6 2 11.6 3 18.8 4 10.6 5 17.8 6 17.0 7 19.6 8 16.0 46 17.6 47 15.2 43 15.6 49 25.4 51 12.6 52 16.0 53 14.4 I 8.44 . 9.16 w S.E. 0.68 - 0.67 Statistical analysis: group 1 vs 2: p 0.01; group C vs 2: p group 1 vs 4: p 0.001; group C vs 3: p 0.001. 13.70 1.33 0.005; group 1 vs 3: p 15.36 0.80 0.001; group C vs 4: p 15.79 1.0S 0.001; SL 070450 Table 27 - Hematocrit value in Control and ECD-exposed rats CONTROL Rat No. % GROUP 1 Rat No. % 801 802 803 804 SOS 807 80S 809 810 847 S48 849 850 851 '852 853 40 43 42 44 41 29 39 41 39 - 40 48 48 35 47 43 601 602 603 604 605 606 ` 607 608 646 647 648 649 650 651 652 653 46 47 41 46 46 39 48 46 46 37 48 46 41 49 - 38 X' S.E. 41.27 1.27 44.27 1.02 Statistical analysis: group 1 vs 4: p 0.01 GROUP 2 Rat No. % 401 402 . 403 404 405 406 407 40S 446 447 448 449 450 451 452 453 36 41 38 41 36 40 38 41 47 40 45 39 40 46 44 49 41.31 0.96 GROUP 3 Rat No. % 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 43 41 38 36 40 38 44 46 47 47 41 46 - 44 46 37 42.27 0.99 . ... * *' GROUP 4 Rat No.. i 1 2 3 4 5 6 7 8 46 47 43 49 51 52 53 i 37 38 ' 40 42 38 35 39 38 45 44 41 45 38 45 44 40.55 0.90 SL 070451 Table 28 - Hemoglobin concentration in Control and ECD-exposed rats. CON'rROL Rat No. % GROUP 1 Rat No. % GROUP 2 Rat No. i GROUP 3 Rat No. % GROUP 4 Rat No.. l 801 13.4 802 14.2 803 14.8 804 14.2 805 13.2 807 13.6 808 14.0 809 13.6 810 14.6 847 14.6 848 14.2 849 16.0 850 15.4 851 14.4 852 15.4 853 14.4 pt 601 602 603 604 605 606 ` 607 608 646 647 648 649 650 651 652 653 . 14.8 15.4 14.8 14.6 15.6 14.6 15.4 15.6 15.2 14.2 15.4 15.4 14.8 15.4 15.8 15.0 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453 X S.E. 14.44 0.20 15.13 0.11 Statistical analysis: group C vs 2: p 0.05; group C vs 3: p 14.2 14.8 13.8 15.0 13.8 15.0 14.6 15.0 15.2 16.0 15.8 15.0 14.6 15.6 15.0 16.0 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 15.0 14.8 14.0 13.8 14.4 14.0 15.0 14.8 15.4 16.0 15.2 15.8 15.0 15.6 15.6 15.2 1 !' 3 4 5 6. 7 8 4 47 48 49 51 52 S3 14.96 0.17 0.05; group C vs 1: p 14.98 0.16 0.005; group C vs 4: p 0.05 14.8 14.0 14.8 14.6 14.2 IS. 6 14.6 14.2 15.0 15.6 14.4 16.0 15.2 16.0 15.6 14.99 0.18 SL 070452 Tabic 29 - Erythrocyte numbers in Control and ECD-*exposed rats CONTROL Rat No. \o oir-- X GROUP 1 Rat No. (x 106) ducup 2 Rat No. (x 106) 801 5.7 802 6.8 803 7.3 804 6.5 808 6.4 807 6.3 808 6.2 809 6.4 S10 7.0 847 6.9 848 6.8 849 7.4 850 7.5 851 7.2 852 7.0 853 7.0 601 602 603 604 605 606 ' 607 608 646 647 648 649 650 651 652 653 6.9 6.9 6.9 7.0 7.4 6.9 7.4 7.4 7.1 6.8 '7.4 7.3 7.1 - 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453 6.9 7.2 6.8 - 6.8 - 7.2 - 7.1 6.9 6.5 6.8 7.3 - 6.2 wY i- S.E. 6.78 0.12 7.11 0.06 6.88 0.19 o q No statistically significant differences were found between the groups. GROUP 3 Rat No. (x 106) 201 202 203 204 205 206 207 208 246 247 n 248 249 250 251 252 253 6.2 7.3 - 7.4 6.8 6.5 7.2 7.2 - 7.3 6.9 6.7 7.5 - 6.3 6.94 0.13 GROUP 4 Rat No.. (x 106) 1 7.3 23 6.5 4- 5 7.4 6 7.3 7 7.2 8 . 6.8 46 6.9 47 48 - 49 6.2 51 7.1 52 7.3 53 7.5 7.0S 0.12 Table 50 Mean erythrocyte volume in Control and ECD-exposed rats CONTROL Rat No. % GROUP 1 Rat No. \ GROUP 2 Rat No. V 801 802 805 804 805 807 803 809 810 847 848 849 850 851 852 853 67 60 56 63 60 42 60 61 53 50 56 66 65 47 65 58 601 602 603 604 605 606 607 60S 646 647 643 649 650 651 652 653 62 65 57 63 63 54 65 63 63 52 66 66 58 67 28 51 ' 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453 49 55 52 56 50 S4 51 55 65 55 61 52 55 ' 62 . 60 67 X 56.81 58.94 56.19 S.E. 3.47 2.42 1.34 No statistically significant differents were found between the groups. GROUP 3 Rat No. % 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 58 56 51 48 54 52 60 62 64 64 56 62 30 60 .* 63 61 56.31 2.13 GROUP 4 Rat No.. % 1 2 3 4 5 6. 7 8 46 47 48 49 51 52 53 50 52 54 57 51 44 53 52 58 60 56 60 52 51 59 54.CO 1.21 SL 070454 SL 070455 Table 31 - Platelet lumbers in Control and ECD-exposed rats. ,* * ' % CONTROL Rat No. (x 105) GROUP 1 Rat No. (x 103) GROUP 2 Rat No. (x 103) 801 802 803 804 80S 807 80S . 809 810 847 848 849 850 851 852 853 210 84 78 108 205 55 66 191 198 56 275 122 63 131 365 71 601 602 603 604 605 606 ' 607 608 646 647 648 649 650 651 652 653 69 150 263 88 59 181 53 66 98 127 110 72 136 67 59 131 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453 225 83 64 210 142 116 71 105 52 76 39 81 59 310 . 55 131 XS.E. 142.38 22.61 108.04 14.05 113.69 18.81 No statistically significant differences were found between the groups. GROUP 3 Rat No. (x 103) 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 123 S3 86 128 123 158 67 89 121 59 161 106 53 75 107 122 101.94 8.66 GROUP 4 Rat No.. (x 103) 1 101 Z 70 3 86 4 110 5 72 6 210 7 157 8 63 46 198 47 61 48 210 49 58 51 60 52 114 53 158 115.20 14.70 SL 070456 'able 32 - Basophilic leukocytes in Control and ECD exposed rats. v* * x CONTROL t No. % 01 02 03 04 05 07 OS 09 10 17 4S 40 50 51 52 53 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 GROUP i 1 GRCUP 2 Rat No. 1 1 Rat No. % 601 602 603 604 605 606 * 607 603 646 647 648 '649 650 651 652 653 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 401 402 . 403 404 . 405 406 407 408 446 447. 448 449 450 451 452 453 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 GROUP 3 Rat No. % 201 202 203 204 205 206 207 208 246 247 .. 248 249 250 251 252 253 0 0 0 0 0 0 0 0 ,0 0 0 0 0 0 0 0 GROUP 4 Rat No.. \ 1 2 3 4 5 6 7 8 46 47 48 49 51 52 53 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 33 - Eorinophilic leukocytes in Control and ECD-exposed rats. CONTROL lat No. % 801 802 803 804 805 807 SOS 09 310 347 34S 349 350 . >51 ',52 I S3 0 .* 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 tn r o O Lr\ GROUP 1 Rat No. % 601 0 602 1 603 0 604 0 605 2 . 606 0 607 0 60S 0 646 0 647 1 64 S 2 649 0 650 0 651 0 652 3 653 0 GROUP 2 Rat No. % 401 402 403 404 405 406 407 408 446 447 44S 449 450 451 452 453 0 1 0 2 0 0 0 1 0 0 0 0 0 0 0 2 GROUP 3 Rat No. % 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 252 253 1 0 0 1 0 0 0 20 1 0 3 0 0 1 0 GROUP 4 Rat No.. % 12 2` * 0 30 40 50 60 70 80 46 0 47 0 48 0 49 - 0 51 2 52- 1 S3 0 i',c 34 - lAKniciitcd Neutrophilic leukocytes in Control and liCU-exposed rats. CONTROL <at No. i >t jSOl :eo2 "i 18 23 803 17 304 20 '805 16 807 22 80S 18 809 16 810 IS 847 17 SIS 17 849 20 sso 20 851 18 852 21 853 18 GROUP 1 Rat No. % 601 602 603 604 60S 606 ' 607 608 646 647 643 649 650 651 652 653 16 19 16 21 22 21 23 17 16 16 23 15 21 22 18 20 X ; s.e. 18,69 0.52 19.13 0.71 Statistical analysis: group 3,4 : p 0.01 GROUP 2 Rat No. % ' ' 401 402 403 404 405 406 407 403 446 447 443 449 450 451 452 453 17 20 21 20 17 19 16 15 20 17 ^ 18 18 17 18 16 18 17.94 0.42 GROUP 3 Rat No. i 201 202 203 204 205 206 207 208 246 247 * 248 249 250 251 2S2 253 14 16 18 19 21 17 , 19 15 17 19 20 15 14 19 16 22 17.56 0.61 GROUP 4 Rat No.. % 1 16 2 19 3 20 4 16 5 17 6 20 7 16 8 22 46 47 36 48 20 49 17 SI 23 52 20 53 18 20.0G 1.37 SL 070458 J. -j3 - hon requested neutrophilic leukocytos in*Control and ECD-exposed rats. ` *% CONTROL Hat No. % 801 802 BG3 SOI 805 807 60S SOD 310 347 34 S 849 850 851 S52 SS3 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 GROUP 1 Rat No. % 601 602 ' 603 604 . 60S 606 ` 607 603 646 647 648 649 650 651 652 653 0, 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 GROUP 2 Rat No. \ * 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 GROUP 3 * Rat No. % 201 202 . 203 204 205 206 207 208 246 247 * 218 249 250 251 252 253 2 O' 0 0 0 1 0 0 0 o. 0 0 0 0 0 0 GROUP 4 Rat No.. % 11 2 -0 30 40 51 60 71 80 46 0 47 0 48 0 49 o 51 0 52 0 S3 0 SL 070460 1 ^ io - ^..^iiucyce percentages in Controlcand VCD-exposed rats. CONTROL .at No. $ GROUP 1 Rat No. I SOI 81 802 73 803 81 804 80 ' 805 82 807 78 SOS 82 809 84 310 80 847 82 CM 0 83 8 19 79 350 79 851 82 852 79 853 81 601 602 603 604 60^ 606 ` 607 608 646 647 648 649 650 651 652 653 82 80 84 79 76 78 75 81 82 82 75 84 79 77 79 80 X S.E. 80.33 0.64 Statistical analysis: group 2 vs 4: p 79.56 0.72 0.01 GROUP 2 Rat No. I 401 402 403 404 405 406 407 408 446 447 448 449 450 451 452 453. * 82 79 76 78 83 81 84 84 80 81 82 82 83 80 84 80 81.19 0.57 GROUP 3 Rat No. % 201 202 203 204 205 206 207 208 246 247 ' 248 249 250 251 252 253 83 84 82 80 79 81 78 82 82 80 78 82 86 81 81 78 81.06 0.56 GROUP 4 Rat No.. V 1 81 2 80 3 78 4 83 5 82 6 79 7 83 8 78 46 47 62 48 80 49 82 51 75 52 79 r 1 80 i 78.71. 1.41