Document e586dgvjgkJRE6K6VjNq1Xby9

t NCI/ICRDB/OK-80/04 PB80-922904 ONCOLOGY OVERVIEW SELECTED ABSTRACTS ON i i THE CARCINOGENICITY OF VINYL CHLORIDE AND RELATED COMPOUNDS March 4, 1980 U.S. DEPARTMENT OF HEALTH, EDUCATION, AND WELFARE Public Health Service National Institutes of Health National Cancer Institute UCC 106316 A SERVICE OF THE INTERNATIONAL CANCER RESEARCH DATA BANK (ICRDB) 5 ONCOLOGY OVERVIEWS are a service of the International Cancer Research Data Bank (ICRDB) Program of the National Cancer Institute. Each ONCOLOGY OVERVIEW represents a survey of the literature associated with a selected area of cancer research. It contains abstracts of articles which have been selected and organized by researchers associated with the field. The purpose of this OVERVIEW is to facilitate and promote the exchange of information between cancer scientists by keeping them aware of literature related to their research being published by other laboratories throughout the world. The material published in ONCOLOGY OVERVIEWS is obtained from CANCERLINE, an on-line cancer information system created by the ICRDB Program in cooperation with the National Library of Medicine. Abstracts from the CANCERLINE database, which is updated monthly with new articles abstracted from over 3000 journals, can be retrieved via on-line computer terminals at more than 800 locations in the U.S. and other countries. Any organization linked to the MEDLARS/MEDLINE system at the National Library of Medicine has access to the CANCERLINE database. This ONCOLOGY OVERVIEW is sent automatically to researchers in the subject area who are identified on the basis of current research project descriptions submitted to an ICRDB-supported Current Cancer Research Project Analysis Center (CCRESPAQ. We encourage all cancer researchers to provide a complete and current project description to CCRESPAC. If you have not recently supplied such a description to an agency or official representative providing input to this program (i.e., NIH, American Cancer Society, Cancer Center Directors, etc.), you may obtain forms and additional information about data input from the ICRDB Program Office address listed below. ONCOLOGY OVER VIEWS may also be obtained by any requestor at modest cost from the National Technical Information Service (NTIS), 328S Port Royal Road, Springfield, Virginia 22161, U.S.A. This ONCOLOGY OVERVIEW is prepared for the ICRDB Program by the Cancer Information Dissemination and Analysis Center (CIDAC) for Carcinogenesis. Future OVERVIEW editions depend on user needs and satisfaction. If this ONCOLOGY OVERVIEW is of value to you, please send your comments to: CIDAC for Carcinogenesis Franklin Research Center Science Information Services Organization 20th and Race Streets Philadelphia, Pennsylvania 19103 U.S.A. If you would like to receive additional ICRDB publications and learn about other ICRDB information services, please contact: ICRDB Program Office National Cancer Institute Blair Building, Room 114 8300 Colesville Road Silver Spring, Maryland 20910 U.S.A. Each researcher receiving this publication la urged to circulate this OVERVIEW to col leagues, co-workers, and others who are engaged in projects related to the area covered by this publication. ucc 106317 Selected Abstracts On THE CARCINOGENICITY OF VINYL CHLORIDE AND RELATED COMPOUNDS Benjamin L. Van Duuren, Sc.D. Consulting Reviewer A Service of the International Cancer Research Data Bank (ICRDB) Program of the National Cancer Institute UCC 106318 INTRODUCTION TABLE OF CONTENTS ABSTRACT NUMBER EDITORIAL COMMENTARY I. CHEMISTRY AND ANALYSIS OF VINYL CHLORIDE AND RELATED COMPOUNDS.................................................................................................... II. PHARMACOKINETICS A, Uptake. Distribution, and Metabolism of Vinyl Chloride and Related Compounds in Experimental Animals 1. Uptake and Distribution of Vinyl Chloride and Related Compounds in Experimental Animals ......................................................................... 2. Metabolism and Activated Intermediates of Vinyl Chloride and Related Compounds in Experimental Animals ........................ B, Uptake, Distribution, and Metabolism of Vinyl Chloride and Related Compounds in Humans.................................................................................... III. BIOLOGICAL EFFECTS IN BACTERIAL SYSTEMS, DROSOPHILA, AND EXPERIMENTAL ANIMALS A. Acute and Subacute Toxicity of Vinyl Chloride and Related Compounds in Experimental Animals ....................................................................................... B. Morphologic and Biochemical Changes in Experimental Animals Exposed to Vinyl Chloride and Related Compounds .......................................................... C. Mutagenicity of Vinyl Chloride and Related Compounds in Bacterial Systems. Drosophila, and Experimental Animals ................................... D. Teratogenicity of Vinyl Chloride and Related Compounds in Experimental Animals....................................................................................... E. Transforming Activity of Vinyl Chloride and Related Compounds in Animal Cell Cultures............................................................................................................ F. Carcinogenicity of Vinyl Chloride and Related Compounds in Experimental Animals............................................................................................................. G. Factors Modifying Carcinogenicity and Toxicity of Vinyl Chloride and Related Compounds...................................................................................................... IV. STRUCTURE-ACTIVITY RELATIONSHIPS FOR VINYL CHLORIDE AND RELATED COMPOUNDS ................................................................................... V. EPIDEMIOLOGICAL ASSOCIATIONS OF VINYL CHLORIDE AND RELATED COMPOUNDS WITH HUMAN CANCERS ........................................................ VI. CLINICAL AND BIOLOGICAL EFFECTS OF OCCUPATIONAL EXPOSURE TO VINYL CHLORIDE AND RELATED COMPOUNDS A. Morphological Changes in Humans Exposed to Vinyl Chloride and Related Compounds....................................................................................................... B. Cytogenetic Changes in Humans Exposed to Vinyl Chloride and Related Compounds...................................................................................................... C. Teratogenicity of Vinyl Chloride and Related Compounds in Humans ............. D. Other Clinical Manifestations of Occupational Exposure to Vinyl Chloride and Related Compounds .......................................................................................... VII. PUBLIC HEALTH ISSUES, OCCUPATIONAL SAFETY, AND REGULATIONS CONCERNING VINYL CHLORIDE AND RELATED COMPOUNDS ................ VIII. REVIEWS AND OTHER RELATED STUDIES INCLUDING VINYL CHLORIDE AND RELATED COMPOUNDS .......................................................................... 1-11 12-34 35-70 71-76 77-83 84-102 103-145 146-150 151-156 157-183 184-190 191-200 201 231 232-241 242-253 254-256 257-268 269 301 302-334 INDEX TO KEY WORDS IN TITLE AUTHOR INDEX 111 ucc 106319 INTRODUCTION This ONCOLOGY OVERVIEW on the carcinogenicity of vinyl chloride and related compounds includes abstracts selected from the CANCERLINE data base, referencing articles published mainly during 1975-79. Selected information dating back to 1970 is included for historical perspective. These abstracts are currently available in the CANCERLINE computerized data base. The strategy used to retrieve these abstracts can be requested from the CIDAC and entered an any MEDLARS/MEDLINE terminal system to update references in this area. The scope of this OVERVIEW includes articles on the carcinogenicity, teratogenicity, and mutagenicity of vinyl chloride and related compounds including chloroprene, vinylidene chloride, tri- and dichloroethylene, and other analagous halogenated hy drocarbons. Studies of the chemistry and analysis, pharmacokinetics, biological effects in animals and humans, chemical structure and biological activity of vinyl chloride and its analogs are included. Also included are epidemiological investigations as well as public health issues, occupational safety and regulations. Studies of halogenated aromatics and of anesthetics are specifically excluded from this OVERVIEW. This OVERVIEW was prepared by Benjamin L. Van Duurcn, Sc.D. (Editor) of the New York University Medical Center, Institute for Environmental Medicine, and Harriet Glaser Hill, M.S., of the CIDAC for Carcinogenesis Information, The Franklin Research Center. EDITORIAL COMMENTARY Short-chain halogenated hydrocarbons, both saturated and unsaturated, have been synthesized, manufactured and used, mainly in industry, for many decades. In some instances the toxicology of these compounds has been studied sporadically for many years. The demonstration of the carcinogenicity of vinyl chloride (VC) by Viola and associates (1) and the subsequent intensive studies of Maltoni and associates (2) on the inhalation carcinogenicity of VC spurred widespread studies on VC and its analogs. These compounds as a group are very heavily used in industry; several of them are gaseous or volatile liquids, and hence pose potential occupational hazards via inhalation. Skin contact and hence systemic absorption is another route of possible exposure. Because of the relative stability of these compounds and thus their possible environmental dispersion and persistence, they need also to be considered as general environmental pollutants. It is, therefore, not surprising that a number of halohydrocarbons related to VC have been found in air, water and in foodstuffs, although usually at low levels (3,4). These factors have generated interest in these compounds in laboratory studies, in the industrial setting, which includes epidemiologic studies, and in governmental regulatory agencies. For VC and some of its analogs, the U.S. Occupational Safety and Health Administration has set health standards and maximum permissible levels of these agents in the workplace. The U.S. Environmental Protection Agency is actively pursuing the sources and elimination of halogenated hydrocarbon contamination in drinking water supplies and in polluted air. In laboratory studies, some work was done on the predictive value of chemical structure as related to biological activity, particularly carcinogenicity. This particular approach is well exemplified by the prediction that trichloroethylene will be carcinogenic based on its possible metabolism to an epoxide which is an alpha-chloroether (5); several compounds in the alpha-chloroether series are carcinogens, the most notable of which is the human carcinogen bis(chloromethyl)ether (6). Subsequent animal studies proved this prediction concerning the carcinogenicity of trichloroethylene to be correct (7). The use of chemical structure, reactivity and possible metabolic pathways as a predictive method for carcinogenicity has been reviewed (6,8). Other methods of predicting animal or human carcinogenicity include the study of chromosomal effects in animals, mutagenicity in bacterial systems and Drosophila, and the effects of these chemicals in mammalian cell culture. In many instances some of these tests have proven to be valuable indicators of potential animal and human carcinogenicity (3,4,9). Teratogenicity studies and studies of mammalian cell culture transformation with halogenated hydrocarbons have been limited. Transformation in cell culture can be a useful short-term assay for predicting carcinogenicity. This has been borne out in studies with other carcinogens (10). Of particular interest is that chromosomal aberrations and the use of cytology as indicators of potentially deleterious health effects in humans exposed to VC have given informative results. This point is relevant and important in medical surveillance of workers exposed to suspect chemicals. - Studies on the metabolism of halohydrocarbons have been extensive and postulated mechanisms of action even more plentiful (3,4,11). Many studies suggest that these compounds are indirect-acting carcinogens, unlike carcinogenic epoxides and haloethers which are direct-acting agents (12,13). Indirectacting agents that are carcinogenic need to be metabolized to activated carcinogenic intermediates. The most frequently proposed activated carcinogenic intermediates for VC and related compounds are epoxides (3,4,11) although peroxides, free radicals and other species of electrophilic intermediates have also been suggested (3,4,11). Binding studies using halogenated hydrocarbons, usually in in vitro experiments, have been done with a variety of tissue constituents, e.g., DNA, RNA and proteins. However it is at present unclear which of these covalent interactions are critically important in accounting for the carcinogenicity f a given agent. V UCC 106321 Editorial Commentary--Con, The same, however, holds true for many other indirect-acting carcinogens such as the nitrosamines. aflatoxins, aromatic amines, etc., some of which have been under study for 30 years or more. In some instances activated carcinogenic intermediates (ultimate carcinogens) have been suggested for these carcinogens. These proposed intermediates are supported by extensive experimental evidence (14). Such studies still need to be pursued more extensively with VC and its analogs. One of the subject categories in this ONCOLOGY OVERVIEW deals with modifying factors, i.e., materials which enhance or diminish the carcinogenicity of halohydrocarbons. The paucity of information in this area suggests the need for increased emphasis on this aspect. This applies not only for the comp ounds under discussion but for many other carcinogens. This subject has not received the attention it deserves probably because of the complexity of the problem. It is, nevertheless, of great importance in cancer causation since multiple factors are frequently involved in chemical carcinogenesis (15). It is noteworthy that in spite of the hundreds of thousands of workers exposed to VC and the extensive epidemiologic studies, the list of workers with angiosarcomas of the liver clearly linked to exposure to VC is only 65 in.number (16). This number is taken from results of epidemiologic studies in eleven or more countries and it is probably impossible to estimate how many hundreds of workers have been exposed to VC for 20 years or more. This raises the question of the carcinogenic potency of VC relative to such potent carcinogens as bis(chloromethyl)ether, beta-naphthylamine and benzidine. In fact, the epidemiologic data available to date suggest that it is a weak carcinogen compared to these carcinogens. Epidemiologic studies have been carried out on the potential occupational hazard of chloroprene and trichloroethylene as carcinogens. In neither case has the evidence been convincing. Three recent .volumes deal in part or in toto with halohydrocarbons (11,17,18). Two recent reviews cover the carcinogenicity, mutagenicity, other biological effects and epidemiology of VC and seven of its analogs (3) and analogous saturated halohydrocarbons (4). REFERENCES 1. Viola, P.L., Bigotti, A., and Caputo, A.: Oncogenic response of rat skin, lungs, and bones to vinyl chloride. Cancer Res. 31: 516-522, 1971. 2. Maltoni, C., and Lefemine, G.: Carcinogenicity bioassays of vinyl chloride: Current results. Ann. N.Y. Acad. Sci. 246: 195-218, 1975. 3. Fishbein, L.: Potential halogenated industrial carcinogenic and mutagenic chemicals. I. Haiogenated unsaturated hydrocarbons. Sci. Total Envir. 11: 111-161, 1979. 4. Fishbein, L.: Potential halogenated industrial carcinogenic and mutagenic chemicals. II. Halogenated saturated hydrocarbons. Sci. Total Envir. 11: 163-195, 1979. 5. Van Duuren, B.L.. On the possible mechanism of carcinogenic action of vinyl chloride. Ann. N.Y. Acad. Sci. 246: 258-267, 1975. 6. Van Duuren, B.L.: Structural prognostication of carcinogenicity and tumor-enhancing activity in various chemicals. In: Niebergs, H.E. (editor): Prevention and Detection of Cancer. Part 1, Prevention, Vol. 2, Etiology, New York and Basel, Marcel Dekker, Inc., 1978, pp. 2071-2084. 7. National Cancer Institute: Carcinogenesis bioassay of trichloroethylene. Technical report series no. 2, DHEW Publ. No. (NIH) 76-802, 1976. 8. Ashby, J.: Structural analysis as a means of predicting carcinogenic potential. Brit. J. Cancer. 37: 904-923, 1978. 9. Ames, B.N.: Identifying environmental chemicals causing mutation and cancer. Science 204: 587-593, 1979. 10. DiPaolo, J.A. and Castro, B.C.: In Vitro carcinogenesis with cells in early passage. In: Third Decennial Review Conference on Cell Tissue and Organ Culture; Gene Expression and Regulation in Culture Cells, U.S. National Cancer Institute Monograph, 48, pp. 245-259, Bethesda, Md., U.S. National Cancer Institute, 1978. 11. U.S. Dept. Health, Education and Welfare, NIEHS: Conference on comparative metabolism and toxicity of vinyl chloride related compounds. Envir. Health Perspect., Vol. 21,233 pp., DHEW Publ. No. (NIH) 78-218. 1977. 12. Van Duuren, B.L., Goldschmidt, B.M. and Seidman, I.: Carcinogenic Activity of di- and trifunctional alpha-chloro ethers and of l,4-dich!orobutene-2 in ICR/Ha Swiss mice. Cancer Res. 35: 2553-2557, 1975. ` vi UCC 106322 13. Henschler, D,: Metabolism and mutagenicity of halogenated olefins - A comparison of structure and activity. Envir. Hlth. Perspect. 21: 61-64, 1972. 14. Searle, C.E. (ed.), Chemical Carcinogenesis. American Chemical Society Monograph No. 173, American Chemical Society, Washington, D.C., 1976, 788 pp. 15. Van Duuren, B.L., and Goldschmidt, B.M.: Cocarcinogcnic and tumor-promoting agents in tobacco carcinogenesis. J. Natl. Cancer Inst. 56: 1237-1242, 1976. 16. Spirtas, R., and Kaminski, R.: Angiosarcoma of the liver in vinyl chloride - polyvinyl chloride workers: 1977 Update of the NIOSH register. J. Occup. Med. 20: 427-429, 1978. 17. Selikoff, I.J., and Hammond, E.C. (eds.): Toxicity of vinyl chloride - polyvinyl chloride. Ann. N.Y. Acad. Sci, 337 pp., Vol. 246, 1975. 18. Saffiotti, U., and Wagoner, J.K. (eds.): Occupational carcinogenesis. Ann. N.Y. Acad, Sci., Vol. 271, 516 pp., 1976. vn UCC 106323 I. CHEMISTRY AND ANALYSIS OF VINYL CHLORIDE AND RELATED COMPOUNDS I. A PRACTICAL METHOD FOR THE MEASUREMENT OF VINYL CHLORIDE MONOMER (VCM) IN AIR. Murdoch IA. Hammond AR Standard Telecommunication Lab. Limited, Harlow, Essex, England Ann Occup Hyg; 20( I):55-6l 1977 A new method for the determination of vinyl chloride monomer in air is described which uses gas chromatography and a sealable trap sampling system. For practical purposes the detection limit is 0.1 vol/million, since below this level there is a possibility of peaks from minor impurities. (3 Refs) 2. MEASUREMENT OF SOME POTENTIALLY HAZ ARDOUS MATERIALS IN THE ATMOSPHERE OF RUB BER FACTORIES. Nutt A Dunlop Res. Centre. Birmingham, England Environ Health Perspect; 17.117-123 1976 The measurement of chlorinated monomers in polyvinyl chloride (PVC) and polychloroprene, and the measurement of benzopyrene (BP) in factory air, particularly in the tire industry, are outlined. BP is present in trace quantities in the mineral oils and carbon blacks used in tire manufacture. Measurements taken daily over a 2-yr period showed no significant concentrations of BP in the atmosphere within the tire plant as compared to an outside air station. (6 Refs) 3. MIGRATION OF VINYL CHLORIDE FROM PVC PACKAGINGS. VomBruck CG, Eckert WR, Rudolph FB Unilever Gesellschaft mbH, Hamburg, West Germany Fette Siefen Anstrichm; 78<8):334-337 1976 Gas chromatographic assay of vinyl chloride (VC) migration in polyvinyl chloride (PVC) or fat simulant HB 307 was made by Puschmann's method with a sensitivity of 0.00001% (0.1 mg/kg) for PVC and 0.000001% (0.01 mg/kg) for HB 307. VC migration from PVC was directly prop ortional to the initial VC concentration. In two separate PVC samples the VC level fell from 0,006% to 0.002% and from 0.0017% to 0.0006% after 35 days. At 20 C a linear relation was also obtained for the VC flow into HB 307 for 100 days. At 40 C a max VC concentration was reached in HB 307 in 50-60 days, after which the level gradually fell. The appearance of a max suggests a reflux from HB 307 through PVC into the ambient atmosphere. On the average, an adult will consume about I kg of packaged food per day. Since only a small fraction of that kilogram is in PVC packaging, the threat of VC contamination, even at a 0.006% concentration in PVC, is very small.. (4 refs) 4. VAPOR INFRARED SPECTROPHOTOMETRIC IDENTIFICATION OF CHLOROFORM, HALOTHANE, METHOXYFLURANE, TETRACHLOROETHYLENE, AND TRICHLOROETHYLENE. Schwartzman G Pharamaceutical Res. and Testing, Food and Drug Admin., Washington, DC, 20204 J Assoc Off Anal Chem; 61(5): 1306-1308 1978 The use of vapor phase infrared spectrophotometry for identification of chloroform, halothane, methoxyflurane, tetrachloroethylene, and trichloroethylene is described. The curves obtained by this technique are unique and characteristic for each material, obviating the need for other physical or chemical tests in the identification of these compounds. (4 Refs) 5. GAS CHROMATOGRAPHIC ANALYSIS OF HALOGENATED HYDROCARBONS IN SILICONE HALIDES. Rath HJ, Schmidt D, Wimmer J Wacker-Chemitronic, Gesellschaft fur Elektronik- Grundstoffe mBH, Postfach 1140, D-8263 Burghausen, W. Germany Fresenius Z Anal Chem: 295(4):266-268 1979 A method for analyzing silicone halide complexes for small amounts of contaminating halogenated hydrocarbons is described. After hydrolysis of the matrix, the hydrocarbons are enriched in the nitrogen phase of a nitrogen-water mixture, and gas chromatography is used to detect the hydrocarbons. Transdichloroethene, 1,1-dichloroethane, 1,1,1trichloroethane, and trichloroethylene are detectable at concentrations of I ppm (by wt). (6 Refs) 6. GAS-LIQUID CHROMATOGRAPHIC DETERMINATION OF TRICHLOROETHYLENE METABOLITES IN URINE. Nomiyama H, Nomiyama K., Uchiki H Dept. Environmental Health, Jichi Medical Sch., Minamikawachi-machi, Tochigi-ken 329-04, Japan Am Ind Hyg Assoc J; 39(6):506-510 1978 A gas-liquid chromatography technique using an electron capture detector was developed to determine all the urinary metabolites of trichloroethylene (TCE) on a single column. With urine from TC E-exposed rats, detection limits were as low as 0.1-5 nanograms. (10 Refs) 7. ETHYLENE DIBROMIDE IN URBAN AIR. Leinster P, Perry R, Young RJ Public Health and Water Resource Engineering Section, Imperial Coll. Science and Technology, London S.W.7, England Atmos Environ; l2(l2):2383-2387 1978 A procedure for the rapid sampling of ethylene dibromide, a potential carcinogen, in ambient air is described. Ambient levels in London air were in the range 0.001-0.17 ug/m3. and levels on a garage forecourt were 1.2 and 1.8 ug/m3. Ethylene dibromide was also measured in car exhaust, a calculation relating levels of organic lead to those of ethylene dibromide is presented. (9 Refs) I UCC 106324 8. REACTIONS OF EPOXY-l,l,2-TRICHLOROETHANE WITH NUCLEOPHILES. Kline SA, Van Duuren BL Lab Organic Chemistry and Carcinogenesis, Inst. Environmental Medicine, New York Univ. Medical Center, New York, NY, 10016 J Heterocyct Chem; 14(3) 455-458 1977 The reactions of epoxy-1,1,2-trichloroethane with nucleo philes were studied. Epoxy-l,l,2-trichloroethane was synthesized by UV or benzoyl peroxide initialed autoxidation of trichloroethylene. The epoxide intermediate of 1,1,2trichloroethylene (TCE) has been proposed as the activated carcinogenic intermediate of the compound. The reactivity of epoxy-i.l.2-inchloroethane toward sulfhydryl nucleophiles was determined since the SH moiety is a likly target for its covalent reactions with protein. It reacted readily with 2mercaptobenzimidazole, l-methyl-2-mercaptoimidazole, pnitrothiophenol, and 3,4-dichlorothiophenol forming 2chloro-2-(benzimidazole-2-thio) acetic acid, 2-chloro-2-(lmethylimidazole-2-thio) acetic acid, 2-chloro-2-(4nitrothiophenoxy)-4-nitrophenylthioacetate, and 2-chloro-2(3,4-dichlorothiophenoxy)-3,4-dichlorophenylthioacetate, respectively. Base hydrolysis of 2-chloro-2-(4nitrothiophenoxy)-4-nitrophenylthioacetate yielded 2,2-di(4nitrothiophenoxy)acetic acid. Adduct 2-chloro-2-(4nitrothiothiophenoxy)-4-nitrophenylthioacetate decomposed on silica gel yielding p-nitrophenyldisulfide. (II Refs) 9. SYNTHESIS AND REACTIONS OF CHLOROALKENE EPOXIDES. Kline SA, Solomon JJ, Van Duuren BL Lab. Organic Chemistry and Carcinogenesis, Inst. Environmental Medicine, New York Univ. Medical Center, New York, NY, 10016 J Org Chem: 43< I8):3596-3600 1978 The chloroalkene epoxides, vinyl chloride oxide (I), trichloroethylene oxide (2), tetrachloroethylene oxide (3), cisand trans-l-chloropropene oxide (4 and 5), and cis- and trans1,3-dichloropropene oxide (6 and 7), were synthesized from their respective chloroalkenes via either autooxygenation (in the case of 2 ahd 3) or m-chloroperbenzoic acid oxidation (in the case of I and 4-7). Dichlorobenzene was a byproduct in the synthesis of both 6 and 7. In the case of 6, its formation was determined to be a result of bimolecular reaction involving an intermediate in the synthesis of 6. Kinetics of hydrolysis at pH 7.4 and 37 C were determined for compounds 2-7. Kinetics of thermal decomposition in dilute hydrocarbon soln were determined for compounds 2, 4, 5, and 7. The hydrolysis and thermolysis rates are discussed with respect to structure and mechanism of product formation. (Author abstract) (21 Refs) 10. A HIGHLY STEREOSELECTIVE SYNTHESIS OF VINYL BROMIDES AND CHLORIDES VIA DISUBSTITUTED V1NYLSILANES. Miller RB, McGarvey G Dept, Chemistry, Univ, California, Davis, CA, 95616 J Org Chem: 43(23):4424-443l 1978 A detailed study of the utility of vinylsilanes as intermediates in a stereoselective synthesis of vinyl halides is described. The requisite vinylsilanes are readily available from alkynes by hydroalumination-protonolysis or hydrosilation. Various methods of desilicohalogenation of intermediate dihalides from vinylsilanes 2a and 3a are compared. The effect of the alkyl substituent on the vinylsilane upon yield and stereoselectivity of the overall halogenationdesilicohalogenation sequence is studied. When the substituent is a primary or secondary alkyl group, the vinylsilanes are converted in good yields with high stereoselectivity to the vinyl chlorides and bromides; the overall reaction involves replacement of the tnmethylsilyl group by halogen with inversion of stereochemistry about the double bond. When the substituent is a phenyl group the stereochemical outcome is retention about the double bond. W hen the alkyl substituent is tert-butyl, both isomeric vinylsilanes give cis-l-halo-3,3dimethyl-l-butene as the product. (Author abstract) (32 Refs) 11. VOLATILE CARCINOGENS: OCCURRENCE, FORMATION AND ANALYSIS. (PP. 1943-1949) Hoffmann D. Schmeltz I, Hecht SS, Brunnemann KD, Wynder EL Naylor Dana Inst. Disease Prevention, American Health Foundation, Valhalla, NY, 10595 Prevention and Detection of Cancer, Proceedings of the Third International Symposium on Detection and Prevention of Cancer Held by the International Study Group for the Detection and Prevention of Cancer in New York, April 26 - May 1, 1976. Vol. 2(Part I). International Study Group for the Detection and Prevention of Cancer, New York, NY, 2404 pp,, 1978. Carcinogenicity data (humans and animals): levels in occupational environments, polluted air, and cigarette smoke: and analytical methods for several volatile chemical carcinogens, including vinyl chloride, chlorinated hy drocarbons, bis(chloromethyl)ether, N-nitrosamines, and hy drazines, are reviewed. (33 Refs) II. PHARMACOKINETICS A. Uptake, Distribution, and Metabolism of Vinyl Chloride and Related Compounds in Experimental Animals 1. Uptake and Distribution of Vinyl Chloride and Related Compounds in Experimental Animals 12. DISPOSITION OF I,2**-I4 C VINYL CHLORIDE IN THE RAT. Bolt HM, Kappus H, Buchter A. Bolt W Institut fur Toxikologie der Universital. Wilhelmstr. 56, D-7400 Tubingen, West Germany Arch Toxicol (Berl); 35(3):153-162 1976 Three male Wistar rats, 200-250 g, were exposed to (1,2**-14 C)-vmyl chloride in an all-glass closed system of 10.3 I volume. To avoid saturation of the metabolizing enzymes, concentrations below 100 ppm were applied. In preliminary experiments, it was found that only about 40% of inspired vinyl chloride is absorbed by the lungs. Uptake of vinyl chloride by the rats was completely blocked by acute pretreatment with potent inhibitors of cytochrome P-450dependent microsomal drug metabolism (i.e, by 35 mg/kg 3bromophenyl-4(5)-imidazole or 50 mg/kg 6-nitro-1,2,3benzothiadiazole in 0.6 ml/kg dimethyl sulfoxide, DMSO). A weaker inhibition was observed after prelreaimcm with 2diethylaminoethyl-2,2-diphenylvalerate;HCI (SKF-525A) or 5.6-dtmethyl-l,2,3-benzothiadiazole (50 mg/kg in 0,6 ml/kg DMSO). Metyrapone did not cause inhibition. Uptake of vinyl chloride was increased by pretreatment with 1,1,1trichloro-2.2-bis(p-chlorophenyl)cthane (DDT) and, to a lesser extent, with clotrimazole. No significant stimulation of uptake was observed after pretreatment with phenobarbital, 3methylcholanthrenc, rifampicin, or chronic ethanol treatment. Immediately after exposure, highest radioactivity levels were observed in liver and kidney; the radioactive metabolites were rapidly excreted, mainly in the urine (69.4% 6 2.6% within 24 hr). 2 UCC 106325 13. FATE OF 14 C VINYL CHLORIDE FOLLOWING INHALATION EXPOSURE IN RATS. Watanabe PG, McGowan GR, Madrid EO, Gehring PJ Toxicology Res. Lab., Health and Environmental Res., Midland, Ml 48640 Toxicol Appl Pharmacol; 37(l):49-59 1976 The fate of inhaled 14 C-vinyl chloride (VC) at different exposure concentrations was studied in rats. Male rats were exposed to 10 or 1,000 ppm 14 C-VC for 6 hr, and the routes and rates of elimination of14 C activity were followed for 72 hr alter termination of exposure. Following exposure to 10 ppm of VC, urinary 14 C activity and expired VC comprised 68% and 2%, respectively, of the recovered radioactivity. After exposure to 1,000 ppm of VC, the proportion of the radioactivity in the urine decreased and that expired as VC increased, representing 56% and 12%, respectively. The pattern of pulmonary elimination of VC per se was described by similar apparent first-order kinetics following 10, or 1,000 ppm with respective half-lives of 20.4 and 22.4 min. 14 C activity in the urine was eliminated in accordance with a twoexponential equation: the half-lives for the initial phase of excretion were 4.6 and 4.1 hr following 10 and 1,000 ppm, respectively. Recovered 14 C activity remaining in the carcass after 72 hr was 14% and 15%. VC per se was not found in tissues. The urinary 14 C activity was separated by highpressure liquid chromatography into three major metabolites corresponding to N-acetyl-S-(2-hydroxyethyl)cysteine, thiodiglycolic acid, and a third unidentified metabolite. The proportions of the urinary metabolites were not markedly influenced by the exposure magnitude. The fate of inhaled 14 CVC was dose-dependent; this is consistent with previous studies on the fate of VC following ingestion as well as inhalation. (13 refs) 14. PRELIMINARY STUDIES OF THE FATE OF INHALED VINYL CHLORIDE MONOMER (VCM) IN RATS. Hefner RE, Watanabe PG, Gehring PJ Health and Environmental Res., Dow Chemical U.S.A., Midland, Mich. 48640 Environ Health Perspect; 11:235-242 1975 Male Sprague-Dawley rats were exposed to vinyl chloride monomer gas (VCM) in a closed recirculating system. The rate at which VCM was removed from the system via metabolism was determined for rats exposed to intial concentrations of VCM ranging from 50-1167 ppm. Upon exposure to initial concentrations of 50-105 ppm, the rate of metabolism was 8.04 plus or minus 3.40 X 10-3 min-l, Upon exposure to initial concentration ranging from 220 to 1167 ppm. the rate constants were less; the mean value being 2.65 plus or minus 1.35 X 10-3 min-l min-l. Regardless of concentration, the disappearance followed apparent first order kinetics. Pretreatment of rats with pyrazole (320 mg/kg) prior to exposure to 65 and 1234 ppm VCM caused 71 and 87% reductions, respectively, in the rate of metabolism. Ethanol (5 ml/kg, 95%) caused 96% and 83% reductions in the rate of VCM metabolism by rats exposed to 56 and 97 ppm VCM, respectively. Ethanol was less effective in blocking the rate of metabolism by rats exposed to high concentrations of VCM; 46 and 36% in rats exposed to 1025 and 1034 ppm VCM, In rats exposed to 65 ppm VCM, SKF-525-A AA(75 mg/Kg) administration caused no inhibition of the rate of VCM metabolism; however, a 19% inhibition was seen in rats exposed to 1038 ppm. The nonprotein sulfhydryl content of the liver (glutathione and cysteine) of rats exposed to 5015,000 ppm VCM was reduced without a relationship to dose. With repeated daily exposure, the degree of reduction was reduced. Preliminary results indicate that the primary metabolites of VCM react with the non- protein sulfhydryl. Final metabolic products excreted in the urine appear to be S(2-hydroxyethyl)cysteine and S- (2-carboxymethyl)cysteine and the respective N-acetyl derivatives. Monochloroacetic acid was identified as another potential metabolite. The results suggest that VCM is readily and extensively metabolized. Metabolism via the primary pathway, postulated to involve alcohol dehydrogenase, is swamped by exposures to concentrations exceeding 220 ppm. In rats exposed to concentrations at and exceeding this level, metabolism occurs via a secondary pathway(s), postulated to be epoxidation and/or peroxidation. These results are considered pertinent in assessing the potential hazard at low-level exposures to VCM. 15. FATE OF 14 CVINYL CHLORIDE AFTER SINGLE ORAL ADMINISTRATION IN RATS. Watanabe PG, McGowan GR, Gehring PJ Toxicology Res. Lab., Dow Chemical Co., Midland, MI 48640 Toxicol Appl Pharmacol; 36(2):339-352 1976 Male Sprague-Dawley rats were given single doses of 0.05, l.and 100 mg/kg po of14 C-vinyl chloride (VC), and the routes and rates of elimination of 14 C activity were followed for 72 hr. Following 0.05 and t mg/kg, excretion in the urine as nonvolatile metabolites and as 14 C02 in expired air acc ounted for 59%-68% and 9%-l3%, respectively, of the administered dose. Only t%-2% of the dose was expired by the lungs as VC. Conversely, after 100 mg/kg, 67% of the dose was eliminated by the lungs as VC, and urinary nonvolatile metabolites and 14 C02 comprised 11% and 3%, respectively. Pulmonary elimination after 100 mg/kg showed an apparent biphasic clearance with half-times (tl /2 ) of 14.4 and 40.8 min for the respective fast and slow phases. Following 0.05 and I mg/kg, the pulmonary clearance of VC was monophasic, with tl /2 of 53.3 and 57.8,min. The percentage of the dose remaining in the carcass after 72 hr was 10%, 11%, and 2% of the 0.05-, 1-, and 100-mg/kg doses, respectively. The urinary radioactivity was separated by high-pressure liquid chromato graphy into three major metabolites. Two of the three major urinary metabolites were identified as N-acetyl-S-(2hydroxyethyl)cysteine and thiodiglycolic acid by gas chromatography-mass spectrometry. Tlie proportions of the urinary metabolites were not influenced by dose. The fate of doses of 1-100 mg/kg VC was clearly dose-dependent. The results suggest that the metabolism of VC is a saturable process. (20 refs) 16. UPTAKE AND RATE OF METABOLISM OF VINYL CHLORIDE BY THE ISOLATED PERFUSED RAT LIVER PREPARATION. Radwan Z, Henschler D Inst. Toxicology, Univ. Wurzburg, Versbacher Landstrasse 9, D-8700 Wurzburg, W. Germany Int Arch Arbeitsmed; 40(2);I0I-110 1977 The metabolism of vinyl chloride (VC) under controlled, steady-state exposure conditions in varying concentrations was examined in the isolated perfused rat liver. The solubility of VC in the RBC perfusion medium at 37 C was constant from 50 to 25,000 ppm. The amount metabolized. (14.6%) as determined by the difference between VC concentrations before and after passage of the liver, was also constant throughout this concentration range. This indicates that there is no saturation of those enzymes that initiate metabolic conversion of VC. Ethanol (constant addition to 12 mM) and pyrazole (single addition to 200 uM) reduced VC metabolism by 12.7% and 31.6%, respectively. Bromobenzothiazole also inhibited metabolism (48.9%), SKF 525A was inactive, and phenobarbital pretreatment increased the conversion rate by 20.9%. Fasted animals showed a 31.2% increase in the metabolic conversion rate. Determination of SCOT, SGPT, and the lactate/pyruvate coefficient revealed no VC-induced 3 UCC 106326 changes, even at the highest concentration tested (24,000 ppm), but slight liver damage was detectable after increased metabolic VC transformation. This suggests the formation of a reactive intermediate, an epoxide, as a result of the first-step oxidation. The epoxide would be expected if the oxidation were catalyzed by cytochrome P-450. The involvement of other oxidases, however, cannot be ruled out. (19 Refs) radioactivity incorporated into proteins was mostly detected in the liver, lung, kidney, and spleen. VC radioactivity was also incorporated into DNA and RNA isolated from rat liver. These results support the view that VC-induced carcinogenicity is caused by the alkylation of nucleic acids and/or proteins by a reactive metabolite of VC. (0 Refs) 17. HEPATIC MACROMOLECULAR BINDING FOL LOWING EXPOSURE TO VINYL CHLORIDE. Watanabe PG, Zempel JA, Pegg DG, Gehring PJ Toxicology Res. Lab., Health and Environmental Res., 1803 Building, Dow Chemical Co., Midland, Ml, 48640 Toxicol Appl Pharmacol; 44(3):57l-579 1978 Male Sprague-Dawley rats were exposed by inhalation to I. 25, 50, 250, 1,000. or 5,000 ppm "C-labeled vinyl chloride (VC) for 6 hr, and covalent binding of VC to hepatic macromolecules and nucleic acids was studied to determine if VC-induced carcinogenesis might be related to electrophilic alkylation of macromolecules in vivo. The total amount of VC metabolized and hepatic glutathione (GSH) content were also measured. The total amount of radioactivity bound to hepatic macromolecules did not increase proportionately to the increase in the exposure concentration of VC, but it was related directly to the total amount of VC metabolized. At exposure concentrations greater than 50 ppm, the total metabolism of VC and covalent binding appeared to correlate with the induction of hepatic angiocarcinomas in the rats, isolation of RNA and DNA by a nondigestive procedure from the liver of rats exposed to I, 100, 250, and 1,000 ppm VC failed to reveal any detectable radioactivity. Hepatic GSH was depressed significantly only at greater than or equal to 100 ppm, suggesting that VC carcinogenicity is related to a decreased ability to' detoxify the reactive metabolites of VC. The results do not associate the carcinogenic effect of VC with a disproportionate increase in binding of electrophilic metabolites of VC to hepatic macromolecules as the exposure concentration is increased. Moreover, the lack of preferential binding of the metabolites to the hepatocyte nucleic acids sug gests that the carcinogenicity of VC may not be associated directly with this commonly accepted mechanism of carcinogenesis. (27 Refs) 18. COVALENT BINDING OF l4C-VINYL CHLORIDE TO PROTEINS AND NUCLEIC ACIDS IN VITRO AND IN VIVO (MEETING ABSTRACT). Kappus H, Kaufmann R, Appel KE, Bolt HM Inst. Toxicology. Univ. Tubingen, Wilhelmstr. 56, Tubingen. W. Germany Arch Pharmacol; 293(Suppl): R 64 1976 When rat liver microsomes were incubated in atmospheric air containing CI4-vinyl chloride (VC) gas, a max of 0.5 nanomoles of VC metabolites was covalently bound to microsomal protein. RNA and sulfhydryl-containing proteins bound VC metabolites when added to the incubation medium. All these microsome binding reactions could be inhibited by Inaphihyl-4(5)-imidazole and CO. The addition of glutathione plus cytoplasmic fractions decreased the covalent binding of VC metabolites to microsomal proteins while the total metabolism of VC during incubation was enhanced. There was a two-fold increase in the covalent binding of VC metabolites to microsomal proteins when trichloropropene oxide was in the medium. The results support the concept that chloroethylenc oxide formed by microsomal enzymes via an epoxidation step is involved in covalent binding of VC to proteins. After exposure of rats to CI4-VC gas, VC-derived 19. LIVER MICROSOMAL UPTAKE OF "C VINYL CHLORIDE AND TRANSFORMATION TO PROTEIN ALKYLATING METABOLITES IN VITRO. Kappus H, Bolt HM, Buchter A, Bolt W Inst. Toxicology, Univ. Tubingen, W. Germany Toxicol Appl Pharmacol; 37(3);461 -471 1976 Microsomal uptake and irreversible binding of vinyl chloride (VC) radioactivity were determined in Wistar rat liver microsomes incubated with l,2-'4C vinyl chloride, gas in an all glass vacuum system. Both the uptake of VC by microsomes and the alkylation of proteins by VC were dependent on incubation time, enzymatically active microsomes, NADPH, oxygen, and the partial pressure of VC in the atmosphere, and could be inhibited by carbon monoxide. Incubation in the presence of NADPH resulted in a 10-x increase in the amount of VC taken up by microsomes. Uptake of VC by albumin so lutions and liposomal suspensions was one-third to one-fourth of the microsomal uptake in the absence of N A DPH. Addition of glutathione and cytoplasmic fractions to microsomal incubations with NADPH resulted in an increase in VC uptake and a decrease in protein alkylation by VC metabolites. Trichloropropene oxide had no effect on microsomal VC uptake but caused a 2-fold increase in the amount of protein bound by VC metabolites. The results are consistent with the involvement of chloroethylenc oxide as the primary microsomal metabolite of VC capable of reacting with proteins. (31 Refs) 20. PHARMACODYNAMICS AND UPTAKE OF VINYL CHLORIDE MONOMER ADMINISTERED BY VARIOUS ROUTES TO RATS. Withey JR Bureau Chemical Safety 5IFoods5l, Health Protection Branch, Ottawa, Canada J Toxicol Environ Health; 1(3):381-394 1976 To assess the hazard presented by the oral ingestion of vinyl chloride monomer, rats that had been surgically prepared with an indwelling jugular cannula were dosed by intragastric intubation with aqueous solutions containing up to 2.0 mg/ml vinyl chloride. Time-concentration curves were obtained from sequential samples of blood. The uptake of vinyl chloride by this route was found to be extremely rapid; peak concentrations were achieved less than 10 min after administration of the dose. Elimination from the blood compartment appeared to be biexponential. The distribution and elimination rates of vinyl chloride from the blood were also determined from the blood concentration data after the administration of an intravenous dose of aqueous or vegetable oil solution. Studies with the same animal model in a single restraint cage that allowed a "head only" exposure to concentrations of vinyl chloride up to 7,000 ppm in the gas phase have shown a similar rapid uptake followed by a plateau blood concentration during several hours of exposure. On removal from the vinyl chloride atmosphere, blood levels fell rapidly to barely detectable concentrations after 2 hr. Thus subsequent monitoring would not be a reliable indicator of exposure. Uptake profiles after oral dosage were extremely variable. 4 UCC 106327 21. PHARMACOKINETICS OF HALOGENATED ETHYLENES (MEETING ABSTRACT). Filser JG, Bolt HM Institut fur Toxikologie, Universitat Tubingen, Wilhelmstrasse 56, D-7400 Tubingen, W. Germany Naunyn Schmiedebergs Arch Pharmakol; 302(Suppl):R22 1978 The present discussion of toxic effqfcts of vinyl chloride raises the question of interpretatjon^df toxicological data. Evidently, biochemical and mechanistic concepts of action of halogenated ethylenes can only be correlated with toxicities observed in vivo, if differences in pharmacokinetics are taken into account. This consideration led to the present investigation. Rats were exposed in a closed system to atmospheric concentrations of fluoroethylene (vinyl fluoride) 1,1-difluoroethylene (vinylidene fluoride), chloroethylene (vinyl chloride), 1,1-dichloroethylene (vinylidene chloride), trans-l ,2-dichloroethylene and cis-1,2-dichloroethylene, trichloroethylene, and bromoethylene (vinyl bromide). Pharmacokinetic analysis was done as previously described. The following principles could be derived. (I) 'Non-linear' (dose-dependent) pharmacokinetics may apply if the organism is exposed to higher concentrations of halogenated ethylenes. This is consistent with the concept of Watanabe, Young and Gehring. In the case of vinyl chloride, it refers to atmospheric concentrations higher than 250 ppm. (2) The equilibrium constant of distribution of the non-metabolized compound (concentration in the animal/concentration in the gas phase) increases from vinyl fluoride to vinyl bromide. (3) The rate of metabolism depends on the structural properties of the individual compound. Trans-l ,2-dichloroethylene and vinylidene fluoride are extremely slowly metabolized, comparable to the rate of metabolism of 1,1,1-trichloroethane (methyl chloroform) which was used as a reference compound. 22. PHARMACOKINETICS OF VINYL CHLORIDE IN THE RAT. Bolt HM, Laib RJ. Kappus H, Buchter A Inst. Toxicology, Univ. Tubingen, Tubingen, W. Germany Toxicology; 7(2):I79-I88 1977 When rats were exposed to "C-vinyl chloride (VC) in a closed system, the VC in the atmosphere equilibrated with that in the animals tissues within 15 min. This course of equilibration was determined in male Wistar rats treated ip with 6-nitro-l,2,3.-benzothiadiazole at a dose (50 mg/kg) sufficient to block the metabolism of 200-1,200 ppm VC for 4 hr. Saturation of the VC-metabolizing enzymes occurred at an atmospheric VC concentration of 250 ppm. Pharmacokinetic analysis showed no significant accumulation of VC or its major metabolites following repeated administration of the compound. The results support the theory that a reactive, short-lived metabolite, which occurs only in low concentrations, may be responsible for the toxic effects of VC. (25 Refs) 23. DOSE-DEPENDENT FATE OF VINYL CHLORIDE AND ITS POSSIBLE RELATIONSHIP TO ONCOGENICITY IN RATS. Watanabe PG, Gehring PJ Toxicology Res. Lab., Health and Environmental Res., Dow Chemical Co., Midland, Ml 48640 Environ Health Perspect; 17:145-152 1977 Studies were made of the disposition of radioactivity from different doses of po administered or inhaled "C-vinyl chloride (VC) in excreta, expired air, and body tissues of treated rats and of nonprotein sulfhydryl levels in the livers of treated rats. The disposition of VC was found to be a function of dose, particularly after administration po. The percentages of administered VC radioactivity in rats treated po with 0.05 mg/kg VC were 1.4%, 9.0%, 68.3%, 2.4%, and 10.1% m exhaled VC, exhaled carbon dioxide, urine, feces, and carcass, respectively; following 100 mg/kg VC, the corresponding values were 66.6%, 2.5%, 10.8%, 0.5%, and 1.8%; following inhalation of 10 ppm VC, the corresponding values were 1.6%, 12.1%, 68%, 4.5%, and 13.9%; following inhalation of 1,000 ppm VC, the corresponding values were 12.3%, 12.3%, 56.3%, 4.2%, and 14.5%. Exposure to 150, 250, 1,000or 2,000 ppm VC caused a progressive depression of the hepatic nonprotein sulhydryl content; exposure to 50 ppm VC for 7 hr produced a small, inconsistent depression; no depression was observed in rats exposed to 10 ppm. Thse results indicate that statistical projections of data from rats exposed to high doses of VC are not valid for predicting effects of low-level exposure, because the metabolism of VC at different dose levels is not the same. (13 Refs) 24. METABOLIC APPROACH TO INDUSTRIAL POISONING: BLOOD KINETICS AND DISTRIBUTION OF "C-VINYLCHLORIDE MONOMER (V.C.M.) (MEETING ABSTRACT). Duprat P, Fabry JP, Gradiski D, Magadur JL INRS, Toxicology Dept., B.P. 27, F-54500 Vandoeuvre, France Acta Pharmacol Toxicol Suppl (Kbh); 41(1): 142-143 1977 Since the lungs (besides the skin) are one of the most imp ortant routes of occupational poisoning, we have developed acute inhalation tests with labeled substances. Rats were exposed (in total enclosure) for 5 min in a small glass pilot unit. This thermoregulated inhalation chamber received a dynamic air flow (200 or 400 liter/hr) into which vinylchloride monomer (VCM) was injected (1 liter/hr). At the exit, congealment methods recovered the waste VCM. For the blo od kinetic studies (5 min inhalation with 10,000 ppm VCM). a permanent catheter had been implanted in the aortic artery and heparinized blood was regularly collected during the 5 min inhalation period and also during 90 min later. The data plotted on a diagram showed particular time variations, which were chosen for autoradiographic studies. New rats have been exposed (5 min inhalation with 20,000 ppm VCM), and 10, 20, 60, 120, 180 min later anesthetized and then frozen. Autoradiographs were then obtained. At 10 min the liver, bile duct, digestive lumen and kidneys contained radioactivity. Later the amount and the distribution of labeled substances (probably both VCM and its metabolites) increased. The urinary system, salivary and lacrimal glands, liver, skin and thymus contained a great deal of labeled molecules. These findings indicate that inhaled VCM is rapidly absorbed through the lungs and immediately accumulated in the liver. Observation of autoradiographs confirms an urinary excretion and probably indicates bile excretion, (no Refs) 25. THREE-STEP AUTORADIOGRAPHY OF ORGANIC SOLVENTS AND PLASTIC MONOMERS TO REGISTER TOTAL RADIOACTIVITY, NON-VOLATILE METABOLITES, AND NON-EXTRACTABLE METABOLITES (MEETING ABSTRACT). Bergman K, Tjalve H Dept. Toxicology, Univ. Uppsala, Box 573, S-751 23 Up psala, Sweden Acta Pharmacol Toxicol Suppl (Kbh); I4(l):22 1977 Whole-body autoradiography was used to study the distribution and fate of organic solvents and plastic monomers in the body. Following the administration of these substances, the animals were frozen in liquid nitrogen. Hemisections of the animals were taken with a saw and exposure was performed in a freeze-box at-80 C. Sections of the animals were then taken on tape, allowing the volatile material to evaporate, and autoradiography was performed at-20 C. The tape-fastened 5 UCC 106328 sections were ihen extracted with water, trichloroacetic acid, and organic solvents and re-exposed at-20 C. This three-step autoradiographic procedure has been applied to distribution studies of organic solvents such as benzene, toluene, xylene, methylene chloride, chloroform, carbon tetrachloride and trichloroethylene and to plastic monomers such as vinylchloride and styrene. A common finding has been the presence of non-metabolized substances in the adipose tissues and the brain. Non-extractable metabolites of some organic solvents and plastic monomers have been found in different tissues, notably in the liver and the kidney. Several distinctive features in the distribution patterns have also been observed for these substances. (I Refs) 26. PHARMACOKINETICS OF VINYLIDENE CHLORIDE IN THE RAT. McKenna MJ, Watanabe PG, Gehring PJ Toxicology Res. Lab., Health and Environmental Res., Dow Chemical Co., Midland, Ml, 48640 Environ Health Perspect; 21:99-105 1977 The effects of inhaled vinylidene chloride (VDC; 1,1dichloroethylene) were determined in rats and mice. In male Sprague-Dawley fasted and fed rats exposed to either 10 or 200 ppm VDC for 6 hr, fasting augmented the process whereby reactive intermediates formed from VDC induced tissue damage. Rats exposed to 200 ppm labeled VDC exhaled a greater percentage of their acquired body burden of radioactivity as unchanged VDC than did animals exposed to 10 ppm. However, rats exposed to the higher concentration showed a greater percentage of body burden remaining in the carcass at 72 hr after exposure. Retention of radioactivity in the carcass was greater in fasted rats exposed to 200 ppm VDC, despite a smaller fraction of the body burden biotransformed by fasted rats. The presence of mercapturic acid derivatives in the urine of exposed rats indicated a major role for glutathione in the detoxification of VDC. Apparently, VDC metabolism represents a balance between biotransformation pathways leading to detoxification by way of glutathione or to covalent binding to tissue nucleophiles and subsequent tissue damage. Enhanced susceptibility to VDC was noted in Ha(ICR) mice exposed to 10 ppm VDC for 6 hr, probably`because of their more rapid metabolism of VDC and greater production of alkylating VDC metabolites over that in the rat. (9 Refs) 27. THE BIOLOGICAL FATE OF VINYLIDENE CHLORIDE IN RATS. Jones BK, Hathway DE Central Toxicology Lab., Imperial Chemical Industries, Ltd., Alderley Park, Cheshire SKI0 4TJ, England Chem Biol Interact; 20(I):27-4I 1978 The fate of vinylidene chloride (DCE) following intragastric (ig), iv, or ip administration to male Alderley Park rats was determined. In rats given 500-ug/kg or 350-mg/kg doses of labeled DCE by all three routes, almost all the radioactivity was recovered within 72 hr of dosing; with 500 ug/kg ig, however, small amounts of label were still being rec overed 72-108 hr after dosing. With the higher ig dose, nearly 70% of the dose was excreted as unchanged DCE and 1% as DCE-related C02 by the lungs; with the lower dose, urinary excretion accounted for 80%, and less than 1% of the unchanged DCE plus 4%-6% of DCE-C02 were eliminated by the lungs. This change in excretion pattern appeared to be due to a saturable drug metabolism and an arterial-alveolar transfer of unchanged DCE from the blood. In comparison, 80% of the small iv dose was excreted unchanged from the blo od within I hr (greater than 60% within 5 min). The excretion pattern after a small ip dose was intermediate between that resulting from iv and that from ig administration. For groups of rats administered various dose levels ig within a 1,300-fold dose range, the plot of the pulmonary excretion of unchanged DCE against the log of the reciprocal doses was biphasic. Radiography following ig doses revealed the presence of large amounts of the "C label in the kidneys and liver after 30 min and a more general distribution of ,JC throughout the soft organs at I hr. The kidneys and liver retained the label for the longest times. Biotransformation resulted in thiodiglycollic acid and an N-acetyl-S-cysteinylacetyl derivative as the major urinary metabolites, together with substantial amounts of chloroacetic acid, dithioglycollic acid, and thioglycollic acid. (12 Refs) 28. DISPOSITION AND METABOLISM OF C 1,1DICHLOROETHYLENE AFTER SINGLE ORAL ADMINISTRATION IN RATS (MEETING ABSTRACT). Reichert D, Werner HW Dept. Toxicology, Univ. Wurzburg, Versbacher Landstrasse 9. D-8700 Wurzburg, W. Germany Naunyn Schmiedebergs Arch Pharmakol; 302(Suppl):R22 1978 1,1-Dichloroethylene (VDC, vinylidene chloride) is converted metaboiically to mutagenic and carcinogenic intermediates. We have investigated the metabolic fate of I4CVDC in rats after single oral doses of 0.5; 5 and 50 mg/kg by collecting and analyzing expired air, urine and feces for 72 hr following administration. The proportions of expired unchanged VDC/l4C02/urinary activity were as follows: 0.5 mg/kg-0.9%/23%/52%; 50 mg/kg-20%/6%/36%. This change in the conversion rate and in the pattern of metabolites is indicative of a rapid saturation of VDC metabolism. This is consistent with previous studies on the uptake and metabolism of VDC by the isolated perfused rat liver. Nonvolatile radioactivity within the body after 72 hr comprised 2-4% of the administered dose and was highest in the liver, the other organs containing minimal amounts only. Thus, most of the VDC is rapidly excreted either unchanged or in the form of polar metabolites. Three major metabolites were separated by thin layer chromatography and gas chromatography, the main part of activity has been identified as thiodiglycolic acid by mass spectrometry. 29. THE USE OF INHALATION TECHNIQUES TO ASSESS THE KINETIC CONSTANTS OF 1,1DICHLOROETHYLENE METABOLISM. Andersen ME, Gargas ML, Jones RA, Jenkins LJ Naval Medical Res, Inst. Toxicology Detachment, Wright- Patterson Air Force Base, OH, 45433 Toxicol Appl Pharmacol; 47(2):395-409 1979 Inhalation techniques were used to assess the kinetic constants of 1,1-dichloroethyiene (1,1-DCE) metabolism. A closed chamber system was developed to study the rate of gas inhalation of rats. By analyzing the time course of uptake curves obtained at various concentrations, the Km (as atmospheric ppm) of the reaction as well as the max velocity (Vmax) of metabolism were determined. Two distinct phases could be traced in the gas phase uptake: whole body equilibrium was represented by a rapid phase with a concentration-independent rate constant of 2.2 hr **-l. The magnitude of the rapid phase was in proportion to the mass of rats in the chamber and the concentration of 1,1-DCE. Rapid phase uptake corresponded to a whole body/gas distribution coefficient of 4.04. The slow phase represented metabolism and was abolished by pretreatment with pyrazolc or simultaneous exposure to CCI4. The acute inhalation toxicity was shown to be a direct function of the amount of metabolite formed, and not of the concentration of 1,1-DCE. Mortality was produced by short-term exposures after formation of 2530 mg of metabolite/kg. At concentrations between 200-1000 ppm, a direct correspondence was found between the time of 6 UCC 106329 exposure required to kill the rats and the time calculated to produce a constant amount of metabolite(s). (34 Refs) 30. TISSUE DISTRIBUTION AND METABOLISM OF 1,2D1BROMOETHANE (MEETING ABSTRACT). Shih TW Kettenng-Meyer Laboratory, Southern Research Institute, Birmingham, Al 35205 Proc Am Assoc Cancer Res: 17():56 1976 1,2-Dibromoethane (DBE), a compound carcinogenic to rats and mice, becomes widely distributed in tissues of treated rats and is a substrate for 2 different enzymes in rat tissues. At I and 3 hr following an intraperitoneal inj of an ethanol so lution of (1,2-'JC)-DBE , radioactivity is concentrated in the liver, kidney, and small intestine. After 24 hr, radioactivity remains in the liver and kidney. At this time, these organs have more radioactivity irreversibly bound to RNA, DNA. and protein than other tissues. In a process possibly related to the in vivo binding of DBE , an enzyme present in rat liver microsomes catalyzes a reaction leading to irreversible binding of radioactivity from DBE to protein in the reaction system. The reaction is dependent upon the presence of TPNH and is stimulated by MgCI2 substrate for a glutathione-S-transferase present in liver, kidney, lung, testes, spleen, and heart of rats. For the liver transferase, the pH optimum is 8.2, and the Michaelis constant for DBE is 25 nM. This reaction presumably is involved in the detoxification of DBE . (Author Abstract) 31. DISTRIBUTION OF METABOLITES OF BENZ0(A)PYRENE IN THE ISOLATED PERFUSED RABBIT LUNG PREPARATION (MEETING ABSTRACT). Dalbey W, Bingham E Dept. Environmental Health, Univ. Cincinnati, Coll. Medicine, Cincinnati, OH Toxicol Appl Pharmacol; 37(I): 189-190 1976 The distribution of metabolites of benzo(a)pyrene in the isolated perfused rabbit lung was investigated. Trichloroethylene was added to the ventilating gas inhaled by the isolated lungs and was evident in the blood after 15 to 30 min of exposure. Chloral hydrate and trichloroethanol glucuronide were not observed. Trichloroethanol in lung tissue was estimated at about 30% of the total metabolites recovered In both rabbit and guinea pig preparations. Trichloroethanol production appeared to be independent of trichloroethylene concentrations; trichloroacetic acid was present in smaller am ounts. Pretreatment of rats with phenobarbital significantly increased trichloroethanol formatiohs in isolated lungs. Ad dition of ethanol to increase blood concentrations did not affect trichloroethanol formation. Although the lungs did show some deterioration with time, trichloroethanol ap pearance was linear over 3 hr. This system appears to be a valid one for the investigation of pulmonary metabolism of trichloroethanol. (no refs) 32. IRREVERSIBLE BINDING OF CHLORINATED ETHYLENES TO MACROMOLECULES. Bolt HM, Filser JO Inst. Toxicology, Univ. Tubingen, Wilhelmstrasse 56, D- 7400 Tubingen-1, W. Germany Environ Health Perspcct: 21:107-112 1977 The binding of vinyl chloride (VC) and trichloroethylene (TCE) to male Wistar rat macromolecules was investigated and compared to that of carbon tetrachloride (CCI4). Saturation of the rat metabolizing systems was achieved at 250* ppm VC, 150 ppm TCE, and 250 ppm CCW. Data on the uptake of the compounds, the urinary excretion of metabolites, and exhalation after exposure indicated that the chlorinated ethylenes were metabolized much faster than CCI4. The metabolites of all three compounds bound irreversibly to tissue proteins, mainly in the liver. Other sites of binding included the kidneys, small intestine, lung, and spleen. Irreversible binding of these compounds ranged within the same order of magnitude when related to the amount of the compound that had been absorbed. No differences in the relative portion of irreversibly bound metabolites were found after exposure of the rats to different atmospheric concentrations of the three compounds. In vitro studies indicated that TCE metabolism by rat liver microsomes in the presence of an NADPH-regenerating system leads to irreversible protein binding, mainly to albumin; similar finings have been reported for VC. Unlike VC, however. TCE metabolites also bind to non-SH proteins such as gamma-globulin and concanavalin A. Thus, TCE metabolites irreversibly bind not only to SH groups of a protein, but also to NH2 groups. (28 Refs) 33. IRREVERSIBLE BINDING OF UC-LABELLED TRICHLOROETHYLENE TO MICE LIVER CONSTITUENTS IN VIVO AND IN VITRO. Uehleke H, Poplawski-Tabarelli S Bundesgesundheitsamt, Abteilung Toxikologie, D-1000 * Berlin 33, W. Germany Arch Toxicol (Berl); 37(4):289-294 1977 IJC-labeled trichloroethylene (10 muMoles/g) administered to male NMRI mice resulted in irreversible binding to the protein of liver fractions. After oral or ip doses of undiluted trichloroethylene, peak liver concentrations were found between 60 and 90 min, with only slightly higher concentrations after ip injection. Covalent binding increased two-fold in microsomes of mice pretreated with phenobarbital. (20 Refs) 34. THE ENVIRONMENTAL FATE OF THREE CARCINOGENS: BENZO(ALPHA)PYREN, BENZ IDINE, AND VINYL CHLORIDE EVALUATED IN LAB ORATORY MODEL ECOSYSTEMS. Lu PY, Metcalf RL, Plummer N, Mandel D Dept. Entomology and Inst. Environmental Studies, Urbana, 1L 61801 Arch Environ Contam Toxicol; 6(2-3); 129-142 1977 The degradation, environmental fate, bioaccumulation, and food chain transfer of radiolabeled benzo(alpha)pyrene (BP), benzidine (BD), and vinyl chloride (VC) were evaluated in two laboratory model ecosystems. In a closed aquatic system, 0.002 ppm BP and 0.008 ppm BD were applied directly to the water and allowed to pass through an aquatic food chain. Their transfer and degradation were observed over a 3day period. In a terrestrial-aquatic ecosystem, 0.2 mg of BP and BD, either alone or with 1.0 mg pipronyl butoxide, were topically applied in acetone solution to Sorghum vulgare seedlings, representing chemical fallout. They were then al lowed to pass through a food chain. The resulting food chain by-products were then allowed to interact in the ecosystem over a 33-day period. It was shown that BP is highly lipophilic and it can bioaccumulate to potentially hazardous levels. This problem was intensified in snails and other organisms deficient in microsomal oxidase, or when there was a presence of mixed function oxidase inhibitors. BD was not bioaccumulated or transfered through food chains to high levels. BD levels were not afTected appreciably by the presence of mixed function oxidase inhibitors. Vinyl chloride did not bioaccumulate or transfer appreciably through the food chains, at least at the ordinary temperatures studied due to its high volatility. There was an excellent correlation between bioaccumulation and octanol/water partition, illustrating that this property and water solubility can be of predictive value in environmental toxicological studies. (19 Refs) 7 UCC 106330 2. Metabolism and Activated Intermediates of Vinyl Chloride and Related Compounds in Experimental Animals The reader may also find the following abstracts of interest: 13, 14, 15, 19, 21, 27, 28, 32. 178, 196 35. THE CHEMISTRY AND BIOGENESIS OF THE S- CONTAININC METABOLITES OF VINYL CHLORIDE IN RATS. Green T, Halhway DE Imperial Chemical Industries Ltd., Central Toxicology Lab., Alderley Park, Cheshire SKI0 4TJ, England Chem Biol Interact; 17(2):137-150 1977 $ The biogenesis of the various urinary S-containing metabolites of vinyl chloride (VC) was investigated in adult male rats. Groups of rats were administered the following chemicals intragastrically: (I) l4C-VC (100 mg/kg; 10 muCi), (2) chloroacetaldehyde (50 mg/kg), (3) S-(2-hydroxyethyl)-Lcysteine (500 mg/kg), or (4) S-(carboxymethyl)-L-cysteine (CMC: 250 mg/kg), and a 24-hr urine sample was taken. Two rats were given repeated daily ip injections of L-(U-'*C) cysteine hydrochloride for 5 days and, 30 min after the final injection, a single dose of VC (100 mg/kg/); a 24-hr urine sample was then taken. Two rats were given a single intragastric dose of ,JC-VC (450 mg/kg) and sacrificed after 45 min; they were dissected and their livers removed. N-Acetyl-S(2-hydroxyethyl)cysteine (AHC) was a major VC metabolite, but, according to the method of protective esterification used, either N-acetyl-S-(2-chloroethyl)cysteine or AHC can be isolated from the body fluids. N-Acetyl-S-vinylcy$teine was a second related metabolite. These S-containing VC metabolites were not mutagenic in S. typhimurium. Administration of the VC metabolites and related compounds to rats indicated that chloroacetaldehyde and CMC, but not chloroacetic acid, are on a pathway connecting VC with thiodiglycollic acid. The fact that chloroacetaldehyde produced both thiodiglycollic acid and AHC in the animal and that CMC was identified among the hydrolytic products from a hepatic extract of VCtreated animals is consistent (I) with the formation of ch loroacetaldehyde and (2> with the reaction of chloroethylenc oxide or chloroacetaldehyde with glutathione in the presence of a glutathione S-epoxide transferase to give the identified Scontaining metabolites. (22 Refs) 36. INTERACTIONS OF VINYL CHLORIDE WITH RATLIVER DNA IN VIVO. Green T, Hathway DE Imperial Chemical Industries Ltd., Central Toxicology Lab., Alderley Park, Cheshire SKI0 4TJ, England Chem Biol Interact; 22(2/3):2l 1-224 1978 The reaction of vinyl chloride (VC) with liver DNA in Wistar rats and the reaction of chloroacetaldehyde (CAA) and calf thymus DNA were investigated 9beta-D-2'Deoxyribofuranosylitnidazo-(2,1 ,i)-purine (ethenodeoxyadenosine) and I beta-D-2*-dcoxyribofuranosyl1,2-dihydro-2-oxoimidazo-( 1,2-cj-pyrimidine (ethenodeoxycytidine) were identified in the enzyme hy drolysates obtained (I) from calf thymus DNA that had been modified by chemical reaction with CAA and (2) from liver DNA prepared from rats that had been exposed po to VC in their drinking water (250 ppm) for approx 2 yr. Thus, VCderived chloroethylene oxide and/or CAA behaved as a bifunctional alkylating agent toward deoxyadenosine and deoxycytidine residues of DNA. It is concluded that modification of the DNA structure by VC, through imidazocyclization of deoxyadenosine and deoxycytidine residues and through depurination of the resulting ethenodeoxyadenosine residues, is related to the mutagenicity of VC. The carcinogenicity of VC in animals and in humans and its mutagenic properties after biotransformation (by microsomal enzymes) into the active metabolites chloroethylene oxide and CAA, which show electrophilic reactivity toward DNA, would appear to conform with the idea of a relationship between carcinogenesis and mutagenesis. (41 Refs) 37. FORMATION OF ETHENO DERIVATIVES OF NUCLEIC ACID BASES IN VIVO BY METABOLITES OF VINYL CHLORIDE (MEETING ABSTRACT). Laib RJ, Ottenwalder H, Bolt HM Inst. Toxikologie, Univ. Tubingen, Wilhelmstr. 56, D-7400 Tubingen, W. Germany Hoppe Seylers Z Physiol Chem; 359(3):293 1978 The detection of mutagenic and carcinogenic effects of vinyl chloride greatly stimulated research on the molecular mechanism of vinyl chloride disease. Rat liver microsomes were incubated with NADPH, l,2-!4Cvinyl chloride and polyadenylic acid or polycytidylic acid. The latter were re isolated from the incubation mixtures and hydrolyzed. The radioactivity originating from 14Cvinyl chloride, which was irreversibly bound to the polyadenylic or polycytidylic acid, was confined to l,N`-ethenoadenosine or 3,N4-ethenocytidine. When rats were exposed to l,2-14Cvinyl chloride, part of the radioactivity was incorporated into the liver RNA. Analysis of hydrolysates of liver RNA showed that all natural nucleosides of RNA were labeled. Besides, small amounts of radioactivity could be detected which were confined to l,N*ethenoadenosine and 3,N4-ethenocytidine. DNA from livers of rats exposed to 1,2-14CvinyI chloride also contained significant amounts of radioactivity. When DNA was incubated with rat liver microsomes, NADPH and 14Cvinyl chloride, radioactivity was also incorporated into the DNA. Re-isolation of the DNA, hydrolysis and separation of the nucleosides showed that radioactive l,N`-etheno-2'deoxyadenosine and 3,N4-etheno-2'-deoxycytidine were formed. The experiments support the theory that vinyl chloride metabolites react with adenine or cytosine moieties of nucleic acids to form etheno analogues. 38. FORMATION OF 3,N4*ETHENOCYTIDINE MOIETIES IN RNA BY VINYL CHLORIDE METABOLITES IN VITRO AND IN VIVO. Laib RJ, Bolt HM Institut fur Toxikologie, Universitat Tubingen, Wilhelmstrasse 56. D-7400 Tubingen, W. Germany Arch Toxicol (Berl); 39(3):235-240 1978 Rats were exposed to l4C-vinyl chloride, and the in vivo liver metabolites were examined. In addition to RNA and I ,N`-ethenoadenosine, 3,N`-ethenocytidine was also radioactively labeled. In vitro experiments using vinyl chloride-exposed liver microsomes and NADPH produced the same results. These alkylation mechanisms are consistent with the mutagenic and carcinogenic properties of vinyl chloride. (22 Refs) 39. FORMATION OF IMIDAZOL DERIVATIVES OF NUCLEIC ACID BASES (DNA AND RNA) BY METABOLITES OF VINYL CHLORIDE IN VIVO AND IN VITRO (MEETING ABSTRACT). (PP. 84) Laib RJ, Bolt HM Inst. Toxicology, Tubingen, W. Germany Fourth Meeting of the European Association for Cancer Research Held at Universite de Lyon, September 13-15, 1977. European Association for Cancer Research, Lyon, France 1977. Rat liver microsomes were incubated with NADPH, 1,2CI4 vinyl chloride and polyadenylic acid or polycytidylic acid. The latter were re-isolated from the incubations and hydrolyz ed. The radioactivity originating from CI4 vinyl chloride, which was irreversibly bound to the polyadenylic or 8 UCC 106331 polycytidylic acid, was confined to I ,N`-ethenoadenosine or 3,N'-ethenocytidine. When rats were exposed to 1,2-04 vinyl chloride, part of the radioactivity was incorporated into liver RNA, Analysis of hydrolysates of liver RNA showed that all natural nucleosides of RNA were labeled. Small amounts of radioactivity could be detected which were confined to l,N`ethenoadenosine and 3,N4-ethenocytidine. DNA of rat liver after exposure of the animals to 1,2-04 vinyl chloride also contained significant amounts of radioactivity. When DNA was incubated with rat liver microsomes, NADPH and 04 vinyl chloride, radioactivity was also incorporated into the DNA. Re-isolation of the DNA, hydrolysis and separation of the nucleosides showed that radioactive l,N`-etheno 2'deoxyadenosine was formed. The experiments support the theory that vinyl chloride metabolites react with adenine or cytosine moieties of nucleic acids to form etheno analogs. These alkylation mechanisms are consistent with the mutagenic properties of vinyl chloride, (no Refs) 40. ALKYLATION OF RNA BY VINYL CHLORIDE METABOLITES IN VITRO AND IN VIVO: FORMATION OF l-N`-ETHENO-ADENOSINE. Laib RJ. Bolt HM Inst. Toxicology, Univ. Tubingen, D-7400 Tubingen-1, W, Germany Toxicology; 8(2): 185-195 1977 The incorporation of I4C radioactivity from vinyl chloride into RNA of liver is described, and the possibility of formation of etheno-adenosine moieties in liver RNA on exposure of rats to I4C vinyl chloride is examined. Rat liver microsomes were incubated with NADPH, 1.2-I4C vinyl chloride and poly-adenosine. Poly-adenosine was reisolated from the incubations and hydrolyzed. I4C vinyl chloride radioactivity was confined to I-N*-etheno-adenosine. When rats were exposed to 1,2-I4C vinyl chloride, part of the radioactivity was incorporated into RNA of liver and exhibited a first maximum, 14 hr, and a second maximum, 72 hr after ending the exposure. Analysis of hydrolysate of liver RNA show,ed that all natural nucleosides of RNA were labeled, and that small amounts of radioactivity were confined to l-Nk-etheno-adenosine. Thus, the experiments support the theory that vinyl chloride metabolites react with adenosine moieties of nucleic acid under formation of l-N`-ethcnoadenosine. The results show that measurement of incorp oration of radioactivity into nucleic acids after exposure of animals to radioactive vinyl chloride is not applicable as a means of determining the alkylating potency of vinyl chloride metabolites towards nucleic acids in vivo. (29 Refs) 41. ALKYLATION OF DNA AND RNA BY METABOLITIES OF VINYL CHLORIDE AND VINYL BROMIDE (MEETING ABSTRACT). Laib RJ, Ottenwaldcr H Institut fur Toxikologie, Universitat Tubingen, Wilhelmstrasse 56, D-7400 Tubingen, W. Germany Naunyn Schmiedebergs Arch Pharmakol; 302(Suppl):R2l 1978 If rats arc exposed to l4C-vinyl chloride, radioactivity is incorporated into nucleic acids of the liver. In previous investigations we showed incorporation of radioactivity from l4C-vinyl chloride into the physiological bases of RNA. In ad dition, alkylation of adenosine and cytidine moieties occurred leading to formation of radioactive l,N'-ethcnoadenosine and 3,N4-ethenocytidine. The time courses of 1,N*ethenoadenosine and 3,N`-ethenocytidine in rat liver RNA after vinyl chloride inhalation are dissimilar 92 hr after ending exposure the ethenoadenosine is only 1/5 of its original value whereas the content of ethenocytidine persists. This ought to be indicative for the relative importance of cytidine alkylation. Formation of labeled etheno derivatives of adenosine and cytidine was also observed if rats were exposed to 14C-vmy! bromide. To establish possible changes in DNA due to alkylation by vinyl chloride metabolites, DNA was incubated with rat liver microsomes, NADPH and 14C-vinyl chloride. Re-isolation of the DNA, hydrolysis and separation of the nucleosides on Ammex-A-6 showed that small amounts of radioactive l,Nl'-etheno 2'deoxyadenosine and 3,N'-etheno 2'deoxycytidine were formed. In addition, a major alkylation product, presumably of 2" deoxyguanosine was isolated which, on Aminex-A-6-columns, showed the same chromatographic behavior as a compound which was obtained from chemical reaction of 2'deoxyguanosine with chloroacetaldehyde. 42. ALKYLATION OF DNA AND PROTEINS IN MICE EXPOSED TO VINYL CHLORIDE. Osterman-Golkar S, Hultmark D, Segerback D, Calleman CJ, Gothe R, Ehrenberg L, Wachmester CA Wallenberg Lab., Univ. Stockholm, Lilia Frescati, S-104 05 Stockholm 50, Sweden Biochem Biophys Res Commun; 76(2>:259-266 1977 Male CBA, BALB, and ATL mice (2-3 mo old) were exposed to various doses (98-160 ppm/hr) of "C-labeled vinyl chloride in glass inhalation chambers for 2-10 hr. and alkylation products recovered from their testes protein, and liver DNA were quantitatively measured. Chromatographic results indicated that vinyl chloride is metabolically activated to form an alkylating agent that introduces the 2-oxoethyl group onto nucleophilic sites; alkylation by chloroethanol was neglible. The alkylation of cysteine and histidine in Hb and of guanine-N-7 in liver DNa varied with the strain of mice used. Considering the sensitivity of the alkylating agents to changes in nucleophilic strength, chloroethylene oxide appeared to be the main reactive metabolite. Male gonads were exposed to the alkylating intermediate, and a risk of heritable damage as well as cancer can be expected. (18 Refs) 43, THE METABOLIC ACTIVATION OF VINYL CHLORIDE IN VITRO (MEETING ABSTRACT), lvanetich KM, Katz ID; Aronson I Dept, Physiology and Medical Biochemistry, Univ. Cape Town Medical Sch., Cape Town, S. Africa Mutat Res; 53(2):204 1978 In the presence of hepatic microsomes, vinyl chloride pro duces a 'Type F difference spectrum and stimulates carbon monoxide inhibitable NADPH consumption. Vinyl chloride, therefore, appears to bind to and to be metabolized by hepatic microsomal cytochrome P-450 in vitro. The Ks value for the binding of vinyl chloride to cytochromes P-450 in uninduced microsomes (80 mM) is decreased to approx 10 mM following phenobarbital or 3-methylcholanthrene induction. The change in Amax and max velocity values are not altered by 3mcthylcholanthrene induction, but are enhanced 2-to 3-fold by phenobarbital induction. These resuts indicate that more one type P-450 cytochrome binds and metabolizes vinyl chloride, but that the cytochrome P-450 induced by phenobarbital plays a major role in these processes. In the presence of NADPH and hepatic microsomes, vinyl chloride mediates the degradation of the heme moiety of cytochrome P-450. Under these conditions, the levels of other hepatic microsomal enz ymes are not affected. The effect of vinyl chloride on the levels of cytochrome P-450 is slight in uninduced or 3mcthylcholanthrene microsomes but is striking in phenobarbital microsomes. The effect is abolished in the absence of NADPH or vinyl chloride and is diminished by reduced glutathione. The activated metabolite of vinyl chloride produced by cytochrome P-450 may in part mediate the mutagenic and carcinogenic effects of vinyl chloride, (no Refs) 9 UCC 106332 44.METABOLISM OF VINYL CHLORIDE: DESTRUCTION OF THE HEME OF HIGHLY PURIFIED LIVER MICROSOMAL CYTOCHROME P-450 BY A METABOLITE. Guengerich FP, Strickland TW Dept, Biochemistry, Vanderbilt Univ. Sch. Medicine, Nashville, TN 37232 Mol Pharmacol; 13(6):993-1004 1977 The NADPH-dependent, vinyl chloride (VC)-mediated destruction of cytochrome P-450 (Cy P-450) was demonstrated in rat liver microsomes and in highly purified reconstituted enzyme systems containing NADPH cytochrome P-450 reductase and cytochrome Cy P-450. This loss of Cy P-450 could be attributed to heme destruction, but not to lipid peroxidation or binding of electrophiles to free sulfhydryl groups. The system required all the components necessary for mixed-function oxidation, including molecular oxygen, and it was inhibited by carbon monoxide, suggesting strongly that oxidative metabolism of VC by Cy P-450 is necessary for destruction. The NADPH Cy P-450 reductase-catalyzed destruction of free and Cy P-450-bound heme was also observed in reconstituted systems in the absence of VC, Inhibition experiments with carbon monoxide and catalase suggested that the VC-mediated destruction of Cy P-450 heme differes from these processes. Two proposed VC metabolites, VC epoxide and 2-chloroacetaldehyde, do not appear to be responsible for the heme destruction. Evidence for the invo lvement of free radicals could not be demonstrated when the reaction was examined by electron paramagnetic resonance spectroscopy or when attempts were made to inhibit Cy P-450 destruction with radical-trapping agents. (56 Refs) 45. THE INTERACTION OF VINYL CHLORIDE WITH RAT HEPATIC MICROSOMAL CYTOCHROME P-450 IN VITRO. lvanetich KM. Aronson 1, Katz ID Dept. Physiology Medical Biochemistry, Univ. Cape Town Medical Sch,, Cape Town, South Africa Biochem Biophys Res Commun; 74(4); 1411-1418 1977 The interaction of vinyl chloride (VC) in vitro with the cytochrome P-450 enzyme system of the hepatic endoplasmic reticulum of Long Evans male rats was investigated. The binding of VC to cytochrome P-450 in vitro was studied spectrally in microsomes from uninduced, 3methylcholanthrene (3-MC)-induced, and phenobarbital (PB)induced rats. VC bound to hepatic microsomal cytochrome P450 in all types of microsomes, with production of a type l difference spectrum. Hanes plots of the spectral binding of VC to cytochrome P-450 were linear for all types of microsomes. VC increased CO- inhibitable NADPH consumption by hepatic microsomes from induced rats. Induction by 3-MC did not increase the apparent max velocity much above that obtained for uninduced microsomes. However, for PBinduced microsomes, max velocity was approx fivefold greater than the apparent max velocity obtained with uninduced microsomes. The effects of inhibitors on the interaction of VC with cytochrome P-450 were determined. SKF 525A fully inhibited the binding of VC to cytochrome P-450, but it did not decrease the enhancement of CO- sensitive NADPH consumption by VC. However, metyrapone did not significantly inhibit the binding of VC to cytochrome P-450 but did inhibit the enhancement of CO- sensitive NADPH consumption by VC. The influence of incubation of hepatic microsomes from induced rats with VC on microsomal enz yme levels was assessed. The levels of cytochrome P-450, cytochrome b5. and NADPH-cytochrome c reductase were not altered after incubation of hepatic microsomes with either VC, NADPH, or NADH. Cytochrome b5 and NADPHcytochrome c reductase were not affected after incubation of hepatic microsomes with VC plus NADPH, but cytochrome P-450 was decreased. The VC-mediated decrease in cytochrome P-450 was slight in control (8%) and 3-MC (7%) microsomes, but was significant in PB microsomes (31%). In PB-induced microsomes, microsomal heme was decreased by approx 30% of the decrease in cytochrome P-450. Reduced glutathione and CO inhibited the VC-mediated decrease in cytochrome P-450 in PB-induced microsomes by approx 30% and 80%, respectively. NADH supported the VC-mediated decrease in cytochrome P-450 by approx 30% in comparison to NADPH. The results may provide an explanation for the observation that prior exposure of laboratory animals to VC protects them against the toxic effects of VC. (17 Refs) 46. CYTOCHROME P-450 AND THE METABOLISM OF VINYL CHLORIDE. Salmon AG Central Toxicology Lab., Imperial Chemical Industries Limited, Alderley Park, North Macclesfield, Cheshire SK10 4TJ, UK Cancer Lett (Amsterdam); 2(2); 109-114 1976 The involvement of cytochrome P-450 in vinyl chloride metabolism was studied. Rat liver microsomes were obtained from male Alderly Park strain SPF albino rats (weighing 150200 g), and U-C!4vinyl chloride (1 mCi/millimoIe) was utiliz ed. Enzymic oxidation of vinyl chloride was assayed by the formation of nonvolatile products. There was a timedependent increase in the amount of label incorporated into nonvolatile water-soluble material. The reaction rate remained constant for 15 min but decreased later, possibly due to exhaustion of substrates and further metabolism of products. Label was not incorporated into either the soluble or solid fractions in the absence of microsomes or NADPH or in the presence of microsomes previously heated to 100 C for 5 min. Addition of I mM reduced glutathione to the reaction mixture increased the microsome-catalyzed formation of nonvolatile water-soluble products. In the absence of active enzymes, there was no reaction of vinyl chloride with glutathione. The concentration of cytochrome P-450 was found to be 0.6 nanoM/mg protein. Addition of 100 microM phenobarbital to a microsome suspension increased absorbance at 390 nanometers and decreased it at 420 nanometers (a typical type 1 difference spectrum). A similar difference spectrum was demonstrated after saturation of a microsomal suspension with vinyl chloride. The type I difference spectrum suggests an interaction with a cytochrome P-450 present in liver microsomes without induction. (12 refs) 47. METABOLISM OF I4C- AND 36CL-LABELED VINYL CHLORIDE IN VIVO AND IN VITRO. Guengerich FP, Watanabc PG Dept. Biochemistry, Center Environmental Toxicology, Vanderbilt Univ, Sch. Medicine, Nashville, TN, 37232 Biochem Pharmacol; 28(5):589-596 1979 Studies were conducted to establish the roles of liver microsomal cytochrome P-450 and epoxide hydratase in the biotransformation of vinyl chloride (VC) to metabolites, particularly those bound to protein and nucleic acids. Label from I4CVC was covalently bound to protein and nucleic acids in vivo and in vitro in the presence of Spraque-Dawley rat liver microsomal fractions or highly purified cytochrome P-450 and NADPH-cytochrome P-450 reductase preparations. The ratio of bound to total nonvolatile metabolites increased in going from the in vivo to the microsomal to the purified system. 36CVC was metabolized by microsomes and highly purified systems: no label was bound, and most of the metabolized chlorine could be accounted for as chloride ion. Phenobarbital pretreatment of rats did not induce total metabolism of VC in vivo at either the 10-or 250-ppm exposure levels; however, binding to protein and RNA was 10 UCC 106333 enhanced at the 10-ppm but not the 250*ppm level. Phenobarbital pretreatment increased the in vitro microsomal conversion of VC to both total and bound metabolites. A siz eable fraction of the label of 14CVC metabolized in vivo was recovered in the microsomal fraction of the liver, but sodium dodecyl sulfate-polyacrylamide gel electrophoresis of in vitro incubations indicated that the metabolites were distributed among many microsomal proteins and not localized to cytochrome P-450. Evidence was obtained for the metabolism of the suspected VC metabolite chloroethylene oxide by microsomal epoxide hydratase. However, the epoxide hydratase inhibitor 3,3,3-trichloropropylene oxide, which blocks the microsomal degradation of chloroethylene oxide, did not enhance the level of VC bound to either protein or adenosine. (39 Refs) 48. VINYL CHLORIDE-MEDIATED CYTOCHROME P450 DESTRUCTION (MEETING ABSTRACT), Strickland TW, Guengerich FP Vanderbilt Univ, Nashville, TN 37232 Fed Proc; 36(3) .991 1977 The vinyl chloride (VC)-mediated destruction of cytochrome P-450 (P-450) and loss of mixed-function oxidase activity were demonstrated in rat liver microsomes. This P-450 destruction was also demonstrated in highly purified reconstituted systems consisting of P-450, NADPH-P-450 reductase (Fp), phosphatidylcholine, deoxycholate, VC, 02, and NADPH; all components were required and destruction was inhibited 80% by the addition of 20% CO, suggesting that oxidative metabolism of VC by P-450 to a reactive metabolite is responsible for the destruction. Loss of heme paralleled P450 destruction while the free sulfhydryl content was not lowered during incubation; several experiments indicate that lipid peroxidation does not play a role in the process. The destruction is not due to VC-epoxide or chloroacetaldehyde. Fp (in the presence of NADPH and 02) also destroys both free heme (ferriprotoporphyrin IX) and P-450 heme in the absence of P-450 substrates. The latter two processes appear to be similar, but distinct from VC-mediated P-450 destruction, as judged by their sensitivity to catalase and insensitivity to CO. (Author Abstract) 49. RESOLUTION OF DOSE-RESPONSE TOXICITY DATA FOR CHEMICALS REQUIRING METABOLIC ACTIVATION: EXAMPLE - VINYL CHLORIDE. Gehring PJ, Watanabe PG, Park CN Health and Environmental Res., Dow Chemical USA, Midland. Ml. 48640 Toxicol Appl Pharmacol; 44(3):58l-59l 1978 The concept that the toxicity of many chemicals may not be a function of exposure to the chemical per se but rather to a biotransformation product is illustrated with vinyl chloride (VC). In such a case, it is necessary to determine the amount of the chemical undergoing biotransformation as a function of dose or exposure before a meaningful dose-response relationship can be established. Male Sprague-Dawley rats were exposed via inhalation to 1.4-4,600 ppm VC for 6 hr, and the total amount of VC metabolized was determined. Activation to the toxic form followed apparent MichaelisMenten kinetics. A logarithmic probability plot of the incidence of hepatic angiosarcoma vs the amount of VC transformed, rather than the exposure concentration of VC, was linear. Assuming no threshold dose, extrapolation of the data below the range of doses causing experimentally observable responses predicted a 0.01% hepatic angiosarcoma incidence in rats exposed to 4.6 ppm VC. Theoretical extension of the extrapolation to humans exposed daily for 8 hr to I ppm led to a predicted incidence of 1.5/100,000,000. Th is incidence, although likely an overestimate in the light of evidence for at least a practical threshold in both rats and humans, is less than that expected to occur spontaneously. Although this method of estimation is not without flaws, the rationale involved represents a new approach that utilizes more logic than current extrapolation methods. The concepts that evolved from this analysis reveal why pharmacokinetics must be considered in designing toxicology experiments as well as in interpreting the resulting data, (22 Refs) 50. COMPARATIVE MAMMALIAN METABOLISM OF VINYL CHLORIDE AND VINYLIDENE CHLORIDE IN RELATION TO ONCOGENIC POTENTIAL. Hathway DE Imperial Chemical Industries Limited, Central Toxicology Lab., Alderley Park, Cheshire SK10 4TJ, England Environ Health Perspect; 21:55-59 1977 Possible mechanisms for the mammalian metabolism of vinyl chloride (VC) and vinylidene chloride (VDC) are discussed in relation to the oncogenic potential of these agents. Studies in rats indicate that VC probably yields chloroethylene oxide (CEO), which is then transformed spontaneously into chloroacetaldehyde (CAA). Since CAA and CEO are mutagenic in the Ames test and in Chinese hamster V79 cells, they may be relevant to VC carcinogenicity. Observations in rats exposed to VC indicate that a degree of DNA depurination occurs. The alkylation that produces imidazoderivative formation with DNA labilizes the N9-purine betaglycoside linkage, which leads to depurination. The gap so produced might then be filled by various bases, resulting in mispairing during DNA replication. In general, there is excel lent agreement between the severe damaging effect of DNA depurination and mutagenicity. Thus, VC would be considered mutagenic/carcinogenic. Experiments with rats exposed to VDC indicate that chloroacetic acid (CA), which is a VDC metabolite per se, lies on a major metabolic pathway for VDC. There is a strong supposition that CA is detoxified through a glutathione S-acyltransferase-catalyzed reaction process and ensuing degradative sequence for the resulting carboxymethylglutathione and that this represents the principal metabolic pathway for CA and a major one for VDC. Thiodiglycollic acid (TDC) is the ultimate detoxification product. Comparative studies of the processing of VDC in mice and rats show that in mice, the metabolic pathway from CA to TDC seems to be readily saturable, possibly on account of an inadequacy in the reaction catalyzed by glutathione S-acyltransferase. Under these circumstances, detoxification of 1,1-dichloroethylene oxide by glutathione Sepoxide transferase and the modification of DNA by 1,1dichloroethylene oxide or chloroacetyi chloride would be expected to be more significant in mice than in rats. This diagnosis of species susceptibility agrees with the discovery of VDC oncogenicity in the kidneys of mice. Overall, VDC emerges as an agent of low oncogenic potential that is likely to be damaging only under special biological circumstances. (26 Refs) 51. STUDIES ON THE METABOLISM OF VINYL CHLORIDE. Antweiler H Universitat Dusseldorf, Dusseldorf, W. Germany Environ Health Perspect; 17:217-219 1976 Current ideas concerning the metabolism of vinyl chloride (VC) in mice and in humans are discussed. It is believed that VC is first metabolized by oxidases to chloroethylene oxide, a strong mutagen. Spontaneous conversion to chloroacetaldehyde (CA) follows; CA may be mutagenic and carcinogenic. Dehydrogenation of CA leads to chloroacetic acid (CAA), a substance of known toxicity but unknown mutagenicity. Conjugation of CAA with glutathione leads to the formation of the principal urinary metabolites of VC; namely S-carboxynicthylcysteine and thiodiacetic acid. CAA II UCC 106334 may also be convened, via glycolic acid, to oxalic acid. (13 Refs) 52. PHARMACOKINETICS OF VINYL CHLORIDE. Bolt HM Inst. Toxicology, Univ. Tubingen, Wilhelmstrasse 56, D7400 Tubingen 1, W. Germany Gen Pharmacol; 9(2) 91-95 1978 Data on the pharmacokinetic behavior of vinyl chloride (VC), all of which have been obtained from studies with rats, are reviewed. Consistent evidence shows that, above a saturation concentration, which on inhalation exposure is reached at 250 ppm VC, nonlinear (dose-dependent) pharmacokinetics apply. Two different models describing the pharmacokinetics of VC have been published. They show that VC can leave the body very rapidly via expiration by the lungs. However, special conditions apply if rats are exposed to atmospheric VC: the compound equilibrates with the organism within 15-30 min and is removed from this equilibrium metabolically, probably by formation of the reactive epoxide. The rapid elimination of VC and its major metabolites from the organism agrees with the current theory that a reactive, short-lived metabolite, which occurs in low concentrations only, may be responsible for the toxic effects of VC. (25 Refs) 53. DETERMINATION OF TWO VINYL CHLORIDE METABOLITES IN URINE (MEETING ABSTRACT). Muller G, Norpoth K Institut fur Staublungenforschung und Arbeitsmedizin, Universitat Munster, Munster, West Germany Naturwissenschaften; 62(ll):541 1975 A ninhydrin-positive compound was detected in the urine of female Wistar rats following the inhalation of vinyl chloride, The retention time of this compound corresponded to that of S-carboxymethylcysteine. Thiodiacetic acid was determined by capillary gas chromatography after its conversion to the dimethyl derivative. The findings indicate the value of determining the urinary thiodiacetic acid concentration ill vinyl chloride-exposed subjects. (4 refs) 54. METABOLISM AND PHARMACOKINETIC PROFILE OF VINYLIDENE CHLORIDE IN RATS FOL LOWING ORAL ADMINSTRATION. McKenna MJ, Zempel JA, Madrid EO, Braun WH, Gehring PJ Toxicology Res. Lab., Dow Chemical Co., Midland, MI, 48640 Toxicol Appl Pharmacol; 45(3):821-835 1978 The pharmacokinetics and metabolism of po ingested vinylidene chloride (VDC) were investigated in SpragueDawley rats. After ingestion of I4C-VDC (I or 50 mg/kg) in corn oil. the animals were housed in metabolism chambers for 72 hr before sacrificing. Rats given 1 mg VDC/kg metabolized and excreted in the urine and feces 78% of the dose, and and exhaled 21% as C02 and 1-3% as unchanged VDC. After ingestion of 50 mg VDC/kg, 19% was expired as unchanged VDC. Fasting had no effect on the elimination of 14C activity in rats given a I mg/kg dose. In those given 50 mg VDC/kg, however, fasting increased the percentage of unchanged expired VDC to 29%, increased the amount of covalently bound I4C-VDC metabolites in the liver, and decreased urinary excretion of nonvolatile metabolites. Results of highpressure liguid chromatography indicated that a major pathway for detoxification was via conjugation with glutathione. VDC metabolism in the rat appears to be a saturable process that is affected by the nutritional state of the animal. (I I Refs) 55. METABOLISM OF TRICHLOROETHYLENE BY THE ISOLATED PERFUSED LUNG. Dalbey W, Bingham E Biology Div., Oak Ridge Natl. Lab., Oak Ridge, TN, 37830 Toxicol Appl Pharmacol; 43(2):267-277 1978 A method for the perfusion of isolated rat and guinea pig lung was developed and used to measure the metabolism of trichloroethylene (TRI). The main features of the perfusion technique included constant-pressure recirculating perfusion with heparinized, autologous, whole blood; cyclic subatmospheric pressure within a temperature-controlled ventilation chamber; and ventilating gas consisting of air containing approx 5% C02. The perfusions lasted up to 3 hr. Lungs were ventilated with 30-45 ppm TRI beginning 20 min after the start of perfusion; additional concentrations ranging from 24-129 ppm were given for guinea pig perfusions. Trichloroethanol (TCE) was noted in the perfusing blood within 15 min of initial exposure. The amount of TCE in the blood increased linearly with time; the rate of TCE appearance was much higher in the guinea pig preparations. Chloral hy drate and trichloroethanol glucuronide were not observed. Pretreatment of the rats with phenabarbital (75 mg/kg for 4 days) significantly increased TCE formation. Addition of ethanol to rat blood preparations did not affect TCE formation. The stability of the TCE appearance and of the physiological and biochemical parameters indicate that this perfusion system can serve as a useful model for investigations of pulmonary metabolism. (17 Refs) 56. MICROSOME-DEPENDENT COVALENT BINDING OF THE CARCINOGEN, TRICHLOROETHYLENE, TO CELLULAR MACROMOLECULES (MEETING ABSTRACT). Banerjee S, Van Duuren BL, Goldschmidt BM Lab of Organic Chemistry and Carcinogenesis, Inst of Environmental Medicine, N Y Univ Medical Center, N.Y., N.Y. 10016 Proc Am Assoc Cancer Res; 18:34 1977 Trichloroethylene (TCE) causes liver cancer in B6C3FI mice, but not in Osborne-Mendel rats (OM). There is higher incidence of tumors in male compared to female mice. In our continuing studies on the in vitro binding of TCE to microsomal protein (MP) (Cancer Res. 36, 2419-2422, 1976), MP from both these mice and rats were incubated with IJCTCE. TCE binds 35-40% more to MP from mice compared to rats. TCE binds to a higher degree (35%) of MP of male compared to female mice. TCE binds to salmon sperm DNA in vitro only if MP from mice are present. TCE interacts 35% more with DNA in the presence of MP from male compared to female mice. TCE binding to DNA or protein in these animals was enhanced by phenobarbital and 3-methylcholanthrene, 3,3,3-Trichloropropane oxide augmented this binding. Thus a metabolite of TCE, eg an epoxide, binds covalently to the macromolecules and there is correlation between such binding and tumor induction. (Author Abstract) 57. COVALENT BINDING OF THE CARCINOGEN TRICHLOROETHYLENE TO HEPATIC MICROSOMAL PROTEINS AND TO EXOGENOUS DNA IN VITRO. Banerjee S, Van Duuren BL Lab. Organic Chemistry and Carcinogenesis, Inst. Environmental Medicine, New York Univ. Medical Center, New York, NY, 10016 Cancer Res; 38(3):776-780 1978 The metabolism of 1,2,2-trichloroethylene (TCE) in species that develop liver tumors following exposure to this carcinogen (B6C3F1 hybrid mice) was compared with that in species resistant to TCE tumor induction (Osborne-Mendel rats). Hepatic microsomes from male B6C3FI mice covalently 12 UCC 106335 bound 46% more TCE that did microsomes from OsborneMendel rats. Furthermore, 30% more TCE was bound to microsomes from mature male Sprague-Dawley rats than im mature rats of the same strain. Binding to microsomal protein was 44% and 63% higher in 5-to 9-wk-old Sprague-Dawley rats than in Osborne-Mendel and Fischer rats of the same age. There was 29% more binding to proteins from male OsborneMendel rats than from females. In B6C3F1 mice TCE bound to stomach, lung, and kidney microsomes as efficiently as to liver microsomes; microsomes from female lung bound 18% more TCE than those from male lung. TCE also covalently bound to DNA, but only in the presence of microsomes. Thirty-seven percent more TCE bound to microsomal protein from male mice than that from female mice, and binding to DNA was 160% greater in the presence of the male microsomes. In vivo pretreatment of mice with 100 mg/kg sodium phenobarbital ip enhanced TCE binding to microsomal protein and DNA by 58% and 41%, respectively. Ip treatment with 30 mg/kg 3-methylcholanthrene increased binding to protein by 31%. At 1.2 mM.trichloropropene oxide increased TCE binding to protein and DNA by 15%; at concentrations greater than 1.2 mM, however, the amount of TCE bound to protein decreased, but there was a constant increase in the binding of TCE to DNA. (30 Refs) 58. INTERACTION BETWEEN TRICHLOROETHYLENE AND HEPATIC ENDOPLASMIC RETICULUM (MEETING ABSTRACT). (PP. 69-70) Banerjee S. Van Duuren BL Laboratory of Organic Chemistry and Carcinogenesis, Institute of Environmental Medicine, New York University Medical Center, New York, N.Y. U.S.A. Third International Symposium On Detection And Prevention Of Cancer. 1976. Trichloroethylene (TCE) is a structural analog of vinyl chloride, which is known to induce angiosarcoma of the liver in rodents and is also a human carcinogen. TCE is a widely used organic solvent in industry and has also been used for many years as an anesthetic. We predicted recently that TCE is likely to ba carcinogenic and that its epoxide probably is the activated carcinogenic intermediate. Subsequently, the National Cancer Institute reported that TCE induces hepatocellular carcinoma and other tumors in B6C3FI hybrid mice. TCE epoxide is expected to be highly reactive toward cellular nucleophiles, eg proteins and nucleic acids. Hence, the microsomal metabolism of TCE and its covalent binding to microsomal protein was examined. Rat liver microsomes were incubated in vitro with "C-TCE. The results showed that TCE binds covalently to microsomal protein since extensive organic extractions and pronasc digestion do not dissociate the TCEprotein complex. The binding was decreased by 7,8benzoflavone, blocked by SK.F-525A and enhanced by intraperitoneal administration of phenobarbital. The possibility that TCE epoxide, once formed, could be converted to water-soluble products through enzymatic hydrolysis by epoxide hydrase was also investigated. Addition of 3,3,3trichforo propane oxide, a potent inhibitor of epoxide hydrase to the incubation system markedly enhanced the binding of TCE. These observations support the view that TCE is metabolized to its epoxide, which is most likely involved in TCE carcinogenesis and toxicity. (Author Abstract) 59. COVALENT INTERACTION OF METABOLITES OF THE CARCINOGEN TRICHLOROETHYLENE IN RAT HEPATIC MICROSOMES. Van Duuren BL, Banerjee S New York Univ. Medical Center, New York, NY 10016 Cancer Res; 36(7):2419-2422 1976 Trichloroethylene (TCE), a structural analog of vinyl chloride, induces hepatocellular carcinoma and other tumors in B6C3F1 hybrid mice. TCE epoxide, a possible metabolite, is expected to be highly reactive toward cellular nucleophiles; e.g, proteins and nucleic acids. Hence, the microsomal metabolism of TCE and its covalent binding to microsomal protein were examined. Rat liver microsomes from male Sprague-Dawley rats were incubated in vitro with 14 C-TCE. The results showed that TCE binds covalently to microsomal protein, since extensive organic extractions and Pronase digestion do not dissociate the TCE-protein complex. The binding was decreased by 7,8-benzoflavone, blocked by 2diethylaminoethvl-2,2-diphenylvalerate;HCI (SKF-525A). and enhanced by ip administration of phenobarbital. The possibility that TCE epoxide, once formed, could be converted to water-soluble products through enzymatic hydrolysis by epoxide hydrase was also investigated. Addition of 3,3,3trichloropropene oxide, a potent inhibitor of epoxide hydrase, to the incubation system markedly enhanced TCE binding. These observations support the view that, in order to bind to protein, it is necessary for TCE to be metabolized to its epoxide, a reactive intermediate that is most likely involved in TCE carcinogenesis and toxicity. 60. SPECTRAL EVIDENCE FOR 2,2,3-TRICHLOROOXIRANE FORMATION DURING MICROSOMAL TRICHLOROETHYLENE OXIDATION (MEETING ABSTRACT). Uehleke H, Poplawski S, Bonse G, Henschler D Dept. Toxicology, German Health Office, D-l Berlin 33, Bundesgesundheitsamt, Postfach, W. Germany Arch Pharmacol; 293(Suppl): R 64 1976 The biotransformation of 1,1,2-trichloroethylene(Tri) to 1,1,1-trichloroacetic acid was suggestive of an epoxide intermediate. Aerobic incubation of Tri with rabbit liver microsomes and NADPH resulted in a different absorption peak of 452 nanometers (nm). Addition of Tri-epoxide (2,2,3trichlorooxirane) to reduced microsomes produced a high difference absorption at 452 nm. Dichloroacetyl chloride was the main thermal rearrangement product of Tri-epoxide and also produced 452 nm absorption in reduced microsomes. The difference absorption was only 20% that evoked by the intermediate formed during incubation of Tri in metabolizing microsomes. The absorption implied epoxide formation in microsomal Tri oxidation. Mice were injected ip with C14labeled Tri. Maximal irreversible binding of 12 nanomole/mg protein and 20 nanomole/mg lipid was observed after 5 hr in the liver microsomal fraction. Irreversible binding of Tri was also followed in aerobic incubates of liver microsomes and NADPH. The oxirane intermediate might be responsible for the mutagenic effects in bacterial test systems and for tumors produced after po Tri administration. (0 Refs) 61. MECHANISM FOR TRICHLOROETHYLENE HEPATOTOXICITY (MEETING ABSTRACT). Allemand H, Pessayre D, Descatoire V, Degott C, Feldmann G, Benhamou JP Unite de Recherches de Physiopathologie Hepatique (INSERM), Hopital Beaujon, Clichy, France Digestion; I6(4):331 1977 Trichloroethylene may produce hepatitis in man; the low incidence of liver lesions and their apparent unpredictability might be consistent with a metabolite-mediated toxicity; this hypothesis was tested in the rat. In vitro C14-trichloroethylene with hepatic microsomes and NADPH resulted in the irreversible binding of a C14-labeled material to microsomal proteins; there was no binding if NADPH was omitted and reduced binding if inhibitors of microsomal drug-metabolizing enzymes were added to the incubation mixture. Phenobarbitalpretreatment of the animals increased (a) cytochrome P-450, (b) in vitro covalent binding to microsomal proteins, (c) in vivo covalent binding to hepatic proteins, and (d) the extent of 13 UCC 106336 liver cell necrosis after administration of trichloroethylene, whereas CoCI2-pretreatment decreased (a) cytochrome P-450, (b) covalent binding, and (c) trichloroethylene hepatotoxicity. In vitro covalent binding to hepatic microsomes was decreased by glutathione. In vivo, trichloroethylene administration depleted hepatic glutathione in normal rats but not in rats whose metabolism was inhibited by piperonyl butoxide. It is concluded that (I) trichloroethylene is transformed into a reactive metabolite which covalently binds either to proteins or to glutathione, (2) binding to proteins produces liver necrosis, and (3) binding to glutathione decreases the amount of metabolite available for binding to proteins. 62. INTERACTION OF POTENTIAL ACTIVATED INTERMEDIATES OF THE CARCINOGEN ETHYLENE DIBROMIDE WITH PROTEIN AND DNA IN VITRO (MEETING ABSTRACT). Kline SA, Banerjee S, Van Duuren BL Lab. Organic Chemistry and Carcinogenesis, Inst. Environmental Medicine, New York Univ. Medical Center, New York, NY, 10016 Proc Am Assoc Cancer Res; 20:86 1979 This study was undertaken to evaluate bromoacetaldehyde (BA) and bromoethanol (BE) as activated metabolites in the microsome mediated covalent binding of the potent carcinogen ethylene dibromide (EDB) to microsomal protein and DNA. BA has been detected by others as a metabolite of EDB in vitro while BE is a likely metabolite. 14C-BA was synthesized from l4C-paraldehyde and I4C-BE was synthesized from 14C-ethyiene oxide. In in vitro experiments, native microsomes from the livers of B6C3F1 mice (susceptible to EDB carcinogenesis) and salmon sperm DNA were incubated with either 14C-BA or 14C-BE in the absence of an NADPH regenerating system or with EDB in the presence of an NADPH regenerating system. Both BA and BE bound covalently to protein and DNA to a much greater extent than did EDB. The extent of binding of BA and BE was unaffected when denatured microsomes were used in place of native microsomes. The rate of binding increased with concentration of BA or BE. BA reacted much more rapidly than did BE. Tljus BA and, to a lesser extent, BE bind to cel lular macromolecules without prior metabolic activation and are to be considered potential activated intermediates in EDB tumorigenesis. (no Refs) 63. BINDING OF THE CARCINOGEN ETHYLENE DIBROMIDE TO CHROMOSOMAL CONSTITUENTS OF FORESTOMACH AND LIVER (MEETING ABSTRACT). Banerjee S, Van Duuren BL Lab. Organic Chemistry and Carcinogenesis, Inst. Environmental Medicine, New York Univ. Medical Center, New York, NY, 10016 . Proc Am Assoc Cancer Res; 20:85 1979 Our earlier studies revealed that the covalent binding of the potent carcinogen ethylene dibromide (EDB), a widely used pesticide, to microsomal protein and salmon sperm DNA was dependent on microsomal metabolism. The present study was undertaken to determine whether EDB can interact with chromosomal constituents of forestomach and liver. Chromatin prepared and characterized from these organs of B6C3FI mice (susceptible to EDB carcinogenesis) were incubated with I4C-EDB in the presence of microsomes and a NADPH regenerating system. After incubation EDB-bound chromatin was isolated and fractionated into DNA, histone and non-histone proteins. EDB was bound to a significantly greater extent to chromatin DNA compared to salmon sperm DNA and to chromatin protein compared to chromatin DNA. Binding to non-histone protein was greater than to histone protein. EDB was bound significantly more to chromatin protein from forestomach, the target organ, than to chromatin protein from liver while the reverse was true for chromatin DNA. Such binding of EDB to chromatin structures probably produces alteration in the function of chromatin and this may be related to its carcinogenic action, (no Refs) 64. MACROMOLECULAR BINDING AND METABOLISM OF THE CARCINOGEN 1,2DIBROMOETHANE. Hill DL. Shih TW, Johnston TP, Struck RF Kettering-Mayer Lab., Southern Res. Inst,, 2000 Ninth Ave., S. Birmingham, AL. 35205 Cancer Res; 38(8):2438-2442 1978 Biochemical mechanisms related to the activation and inactivation of the carcinogen 1,2-dibromoethane (1,2-DBE) were investigated. In rats inoculated ip with 1,2-'*C-1,2-DBE 30.4 muCi; 4.2 micromoles (mumol): 0.2 ml ethanol, measurable amounts of radioactivity became bound to protein, RNA, and DNA of all major tissues. For each macromolecules class, the largest amounts of bound radioactivity were in the liver and kidneys. Optimum conditions for the rat liver glutathione Stransferase that uses 1.2- DBE as a substrate were established. The pH optimum was 8.2, the Km for 1,2-DBE was 25 mM. and Vmax was 2.1 mumol/min/g liver. Considerable enzyme activity was found in rat kidney; detectable activity was present in lung, testis, spleen, and heart, Enzymatic activity leading to the irreversible binding of radioactivity from |4C-I,2DBE to proteins in the reaction system was present in rat liver microsomes. The activity was inducible by phenobarbital but not by benzaanthracene. NADPH was required for activity of both the noninduced and the induced reactions; MgC12 stimulated both reactions. Bromoacetaldehyde, a reactive compound probably involved in the irreversible binding, was identified as a metabolite formed in the reaction system. Radioactivity from l4C-1,2-DBE also became bound to microsomal proteins by a nonemymatic chemical reaction. The enzymatic reaction leading to binding to macromolecules and/or the chemical binding reaction may be involved in the biological activity of 1.2- DBE. (22 Refs) 65. INTERACTION OF ACTIVATED CARCINOGENIC INTERMEDIATES OF ETHYLENE DIHALIDES WITH PROTEIN AND DNA IN MICE AND RATS TISSUES IN VITRO (MEETING ABSTRACT). Banerjee S, Van Duuren BL Lab. Organic Chemistry and Carcinogenesis, Inst. Environmental Medicine, New York Univ, Medical Center. New York, NY, 10016 Proc Am Assoc Cancer Res; 19:67 1978 Ethylene dibromide (EDB) is known to cause cancer of the forestomach in B6C3F1 mice (BM) and Osborne-Mendel rats (OMR). Ethylene dichloride (EDC) is known to cause liver cancer in male B6C3FI mice but not in female mice or OMR. In in vitro experiments microsomal preparations from liver and stomach of BM and OMR were incubated with CI4EDB and salmon sperm DNA. Covalent binding increases with increasing concentrations of microsomal proteins or EDB. There was no binding to DNA in the absence of microsomes. With denatured microsomes, binding to proteins was greater than 4% (p less than 0.01) compared to native microsomes; SKF-525A inhibited the binding to protein and DNA by 80% (p less than 0.02). Binding of CI4-EDC was decreased by 90% (p less than 0.02) by addition of GSH or Imcthyl-2-mercaptoimidazole. CI4-EDC binds 1-2 x more to liver microsomes from male BM compared to female BM or OMR of both sexes. The proposed activated carcinogenic intermediates are halohydrins, epoxides or other related electrophilic and transient species. 14 UCC 106337 66. 1,1-DICHLOROETHYLENE HEPATOTOXICITY: PROPOSED MECHANISM OF ACTION AND DISTRIBUTION AND BINDING OF "C RADIOACTIVITY FOLLOWING INHALATION EXPOSURE IN RATS. Jaeger RJ, Shoner LG, Coffman L Toxicology Unit, Dept. Physiology, Kresge Ceilter Environmental Health, Harvard Sch. Public Health, 665 Huntington Ave., Boston, MA, 02115 Environ Health Perspect; 21:113-119 1977 The mechanism of 1,1-dichloroethylene (1,1-DCE)induced hepatotoxicity and its potentiation by fasting were determined in male Holtzman rats, and results on tissue distribution and binding of radioactivity (RA) were related to the proposed mechanism of action. Trichloropropane epoxide (TCPE: 0.1 m|/kg po), an epoxide hydrase inhibitor, significantly increased the toxicity of 1,1-DCE (100-150 ppm by inhalation, x 4 hr) in 18-hr-fasted (FA) rats, as measured by serum sorbitol dehydrogenase (SDH) elevation. When FA rats were exposed to 250 ppm 1,1-DCE alone, hepatic citric acid levels were increased 416%, SDH activity 636.4-fold (vs increases of 122% and 100-fold in fed (FE) rats). It was hy pothesized that mitochondrial injury was associated with inhibition of the tricarboxylic acid cycle and that monochloroacetic acid is the toxic metabolite of 1,1-DCE. When FE and FA rats were exposed for 2 hr to 2,000 ppm "Cl.l-DCE in a recirculating exposure chamber, there was no difference in rate of uptake between the two groups. FA rats had more water- or trichloroacetic acid (TCA)-soluble 1,1DCE metabolites in their tissues than FE rats. At 30 min postexposure, most of the total RA (mainly TCA-soluble) was detected in the kidneys of both groups. FA rats had more tissue-bound or TCA-insoluble RA in their mitochondrial and microsomal fractions than FE rats, but both groups had ap prox the same rate of disappearance of RA (half-time of 2-3 hr). At 26 hr, the urinary excretion of RA was similar in FE and FA rats (36.6%). The results suggest that although there are no significant metabolic differences between FE and FA rats, there does appear to be a significant difference in the in vivo pathway for the products of metabolism. The fact that mice develop renal tumors after long-term 1,1-DCE exposure but rats only show significant hepatocellular injury is explained. 67. DIFFERENCES IN METABOLISM OF VINYLIDENE CHLORIDE BETWEEN MICE AND RATS. Jones BK, Hathway DE Central Toxicology Lab., Imperial Chemical Industries, Alderley Park, Cheshire SK10 4TJ, England Br J Cancer: 37(3);411-417 1978 The metabolism, of vinylidene chloride (1,1dichloroethylene, DCE) was investigated in male Alderley Park rats and male Alderley Park mice. Following a single po dose of 50 mg/kg. pulmonary excretion of unchanged DCE accounted for 28% of the dose in rats, but for only 6% in mice. This suggested that the efficiency of DCE metabolism follows the known activity of cytochrome P-450 in the organs of mice, that the real exposure (expressed as amount of DCE metabolized) is relatively higher for orally dosed mice than rats, and that DCE is more likely to be carcinogenic in mice than rats. The mice metabolized DCE similarly to rats, but differences were noted. Qualitatively, mice, but not rats, excreted a small amount of N-acetyl-S-(2carboxymethyl)cysteine. Quantitatively, (I) the relative prop ortions of the N-acetyl-S-cysteinylacetyl derivative that were formed in mice and rats paralleled the activity of liver glutathione-S-epoxide transferase, and (2) there were marked differences in the proportions of DCE metabolites belonging to the chloroacetic acid branch of the metabolic pathway. A tracer study verified the beta-thionase hydrolysis of thiodiglycollic acid and the biogenesis of the N-acetyl-Scysteinylacetyi derivative. It is suggested that the metabolites 1,1-dichloroethylene oxide and chloroacetyl chloride may be important in murine DCE carcinogenicity. (19 Refs) 68. DETECTION OF ELECTROPHILIC METABOLITES OF HALOGENATED OLEFINS WITH 4-(4NITROBENZYDPYRIDINE (NBP) OR WITH SALMONELLA TYPH1MURIUM (MEETING ABSTRACT). Barbin A, Planche G, Croisy A, Malaveille C, Bartsch H Unit Chemical Carcinogenesis, International Agency for Res. on Cancer, 150 cours Albert Thomas, 69008 Lyon, France Mutat Res; 53(2):150 1978 Metabolic conversion of volatile substances can be assayed by passing a gaseous mixture of the test compound and oxygen (air) through a mouse liver microsomal system and by trapping volatile aklylating metabolites by reaction with excess NBP. Using this system, epoxide formation from vinyl chloride or vinyl bromide was demonstrated; 2-chloro- 1,3butadiene (chloroprene) yielded an alkylating intermediate although. I.l-difluoroethylene, 1,1-dichloroethylene and trichloroethylene did not. Chloroethylene oxide, perhaps the ultimate carcinogenic metabolite of vinyl chloride, showed potent biological activity in various short-term tests for the detection of potential carcinogens. In all the in vitro assays used, the color reaction with NBP showed the highest sensitivity for detecting chloroethylene oxide at a 2 uM concentration. S typhimurium TA100, in the presence of a 9000 x g superernatant of PB-treated mice, was exposed to gaseous mixtures of the following test compounds/air. Mutation rates (his+ revertant colonies/umol/hr/plate) taken from the linear region of time and dose-dependent assays either with or without NADP+, were as follows: vinyl acetate, 1,1 -difluorethylene and trichloroethylene 0 (0); vinyl chloride 6 (2); 1,1-dichloroethylene 15(1); vinyl bromide 26 (9); 2-chloro1.3-butadiene 51 (9); l-chloro-1,3-butadiene 157 (81); 3,4dichlorobutene-l, 490(345). l,4-Dichlorobutene-2 (77% transisomer), when incorporated in the soft agar layer, showed a mutagenic effect in TAlOO. Microsomal fractions from mouse and human liver enhanced the mutagenicity three-fold. The putative synthetic metabolite 1,4-dichloro-2,3-epoxy butane was, however, four times less mutagenic in TAlOO than in the parent olefin, (no Refs) 69. METABOLISM OF HALOGENATED ETHYLENES. Leibman KC, Ortiz E Dept. Pharmacology and Therapeutics, Univ. Florida Medical Sch., Gainesville. FL, 32610 Environ Health Perspect; 21:91-97 1977 Literature on the metabolic transformations of various chlorinated ethylenes is reviewed, and the results of studies on the products of metabolism of some of these compounds are presented. The metabolism of chlorinated ethylenes may be explained by the formation of chloroethylene epoxides as the first intermediate products. These epoxides appear to rearrange with the migration of chlorine to form chioroacetaldehydes and chloroacetyl chlorides. In experiments in which 1,1-dichloroethylene, trichloroethylene, and tetrachloroethylene were incubated with rat fiver microsomal systems, monochloroacetic acid, chloral hydrate, and trichloroacetic acid, respectively, were found. Rearrangements of chloroethylene glycols formed from the epoxides by hydration may also occur,but they appear, at least in the case of l.l-dichloroethylene, to be quantitatively less important. The unstable epoxide ring formed during the metabolism of chlorinated ethylenes may react with a variety of groups in compounds of biological interest. When these 15 UCC 106338 compounds are essential to cellular function the reaction may lead to alteration of cellular metabolism and subsequent cell necrosis or to carcinogenic or mutagenic events. 70. METABOLISM OF CHLORINATED ALKENES AND ALKANES AS RELATED TO TOXICITY. Henschler D lnstitui fur Pharmakologie und Toxikologie, Univ. Wurz burg, D-87 Wurzburg, W. Germany J Environ Pathol Toxicol; 1(2): 125-133 1977 Chlorine substitution in aliphatic compounds results in a destabilization in alkanes and a stabilization in alkenes. With alkanes, therefore, the main pathways of metabolic transformation to reactive intermediates are radical formation by a C-C break and dechlorination or dehydrochlorination. In the chlorinated ethylenes, the first metabolic transformation step is oxidation to electrophilic oxiranes. These compounds may be hydrolized enzymatically or nonenzymatically, react with cellular nucleophiles, or rearrange to either chlorinated aldehydes or acyl chlorides. With tetrachloroethylene, 1,2-cisdichloroethylene, trans-dichloroethylene, 1,1dichloroethylene, and vinyl chloride, the metabolies identified in in vivo experiments are identical to the thermal rearrangement products of the respective oxiranes. An exception is trichloroethylene, whose thermal rearrangement product is dichloroacetyl chloride; its metabolites in vivo, however, are entirely derived from trichloroacetaldehyde. Mutagenic and carcinogenic activities in the chlorinated ethylenes are determined by the stability of their chlorine substitution compounds: the relatively unstable and unsymmetric oxiranes of trichloroethylene, 1,1-dichloroethylene, and vinyl chloride are mutagenic in the Ames test, but the more stable asymmetric oxiranes of tetra-, 1,2-cis-, and transdichoroethylenes are inactive. (20 Refs) B. Uptake, Distribution, and Metabolism of Vinyl Chloride and Related Compounds in Humans The reader may also find the following abstracts of interest: 49. 53. 116 71. URINARY AND TISSUE GLYCOSAMINOGLYCAN PATTERNS IN ANGIOSARCOMA AND OTHER VINYLCHLORIDE-EXPOSURE-ASSOCIATED LIVER INJURY (MEETING ABSTRACT). (PP. 170) Kupchella CE, Tamburro CH Univ Louisville, Louisville, KY 40208 Third International Symposium On Detection And Prevention Of Cancer. 1976. Both the presence of certain cancers and disorders of connective tissue metabolism are accompanied by alterations in urinary glycosaminoglycan patterns. Because both fibrosis and angiosarcoma of the liver have been connected to vinyl chloride exposure, we evaluated glycosaminoglycan patterns associated with vinyl chloride injury to assess the usefulness of the patterns in early detection. Urines from fifty patients were analyzed for glycosaminoglycans. These included two angiosarcomas, ten cases of vinyl-chloride, work-related, liver injury other than angiosarcoma, and cases of hepatitis, cirrhosis, other cancers, metabolic disorders of the liver, and normal controls. Examined both histochemically and biochemically for glycosaminoglycans were liver tissue samples including two liver angiosarcomas both tumor tissue and non-tumor tissue - five normal livers, and two cirrhotic livers. The urine of patients with vinyl chloride-associated liver injury other than angiosarcoma exhibited a characteristic shift toward the chondroitin sulfates. Tissue analysis revealed up to four-fold greater concentrations of hyaluronic acid and heparin in tumor tissue than in adjacent non-tumor tissue. Angiosarcomatous livers exhibited greater concentrations of glycosaminoglycans than were found in normal livers. Biochemical results confirmed histochemical studies. One patient whose urinary glycosaminoglycan excretion patterns were followed for a two-week period prior to death from liver failure exhibited a pulse of glycosaminoglycan excretion peaking ten days prior to death. Several of these observations appear to have diagnostic significance. (Author Abstract) 72. DETERMINATION OF THIODIGLYCOLIC ACID IN URINE SPECIMENS OF VINYL CHLORIDE EXPOSED WORKERS. Muller G, Norpoth K, Kusters E, Herweg K, Versin E Institut fur Staublungenforschung und Arbeitsmedizin, Westfahliche Wilhelms-Universitat, D-4400 Munster, W. Germany Int Arch Occup Environ Health; 41(3); 199-205 1978 Thiodiglycolic acid (TDGA) content was measured in the urine of workers exposed to varying amounts of vinyl chloride (VC), and an improved analytical method for these determinations was described. The analytical procedure used made it possible to distinguish between normal values (in 20 non-exposed men) and values obtained from 13 slightly exposed workers (0.14-7.0 ppm VCM measured by personal air samplers) at the 1% significance level. In all cases TDGA concentrations exceeding 2.1 microg/ml urine can be distinguished from normal concentrations with a 99% probability of confidence. Mean values obtained from the normal group and two exposed groups suggested a correlation between the extent of exposure and the amount of TDGA excreted. Increasing metabolite concentrations were found in the urine with increasing exposure. The correlation was tested by a distribution free statistical procedure and was confirmed at the level of p less than 0.01. The increase in metabolite excretion began shortly after the initiation of exposure each day and reached a peak in the first or second urine samples obtained after the highest possible VC-uptake. Significant increases in metabolite excretion were observed even at VCconcentrations below 5 ppm. The usefulness of using this analytical procedure for monitoring VC concentration in work places is discussed. (36 Refs) 73. METABOLISM OF TRICHLOROETHYLENE AND TETRACHLOROETHYLENE IN HUMAN SUBJECTS. Ikeda M Dept. Environmental Health, Tohoku Univ. Sch. Medicine, Sendai 980, Japan Environ Health Perspect; 21:239-245 1977 The metabolism of trichloroethylene (3C-E) and tetrachloroethylene, (4C-E) in humans and the urinary concentration of metabolites of these compounds in persons occupationally exposed to their vapors was determined. There was a linear correlation between 3C-E concentration in the work environments and the level of total trichloro compounds in the urine of workers. The trichloroethanol level was also linearly related to 3C-E concentration, but trichloroacetic acid levels deviated from linearity at 3C-E levels greater than 50 ppm. With 4C-E, the trichloroethanol, trichloroacetic acid, and total trichloro compound levels all plateaued at 4C-E levels less than 100 ppm. The respiratory and urinary half lives of 3C-E were 25 and 42 hr, respectively, but those of 4C-E were 65 and 144 hr, respectively. Urinalysis of a 38-yr-old man addicted to 3C-E revealed a urinary half-life of greater than 70 hr. An automated system for the determination of total trichloro compounds in human urine is described. (35 Refs) 16 UCC 106339 74. EVALUATION OF EXPOSURE TO TRICHLOROETHYLENE DURING THE DEGREASING OF SMALL COMPONENTS IN A MACHINERY PLANT. Kalas D Dept. Occupational Diseases, Dobrovskeho 597, 543 01 Vrchlabi, Czechoslovakia Prac Lek; 29(8):299-304 1977 The urinary excretion of trichloroethylene (TRI) and its metabolites, trichloroacetic acid (TCA) and trichloroethanol (TCE), was studied in workers who inhaled TRI in a shop where TRI was used to degrease partially finished metal components. The TRI concentrations measured in the air at three work locations averaged 130-155 mg/cubic meter over the 5-day wk. slightly increasing from the first to the last weekday (from 135-141 to 153-162 mg/cubic meter). The TCE level in the urine rose sharply from less than 200 mg/liter to values around 300 mg/liter on day I of the exposure to drop to about 200 mg/liter on the day following the 5-day work wk, and the TCA level rose from under 100 mg/liter on day 1 to values around 150 mg/liter on the day following the 5-day work wk. The TRI, TCA and TCE levels were highest in the urine portion collected during the last 4 hr of the 7-hr workshifts, and they correlated best with the metabolite levels found in the 24-hr urine samples. (13 Refs) 75. TRICHLOROETHYLENE: RELATIONSHIP OF METABOLITE LEVELS TO ATMOSPHERIC CONCENTRATIONS: PRELIMINARY COM MUNICATION. Smith GF Occupational Health Service of the Post Office, South Western Region, Mercury House, Bond St., Bristol BSI 3TD, England J R Soc Med; 7l(8):59l-595 1978 The amount of trichloroethylene (TRI) inhaled and the levels of its metabolite trichloroacetic acid (TCA) in urine were measured for two subjects continually exposed to TRI while operating a degreasing tank. The total air inspired by the subjects, the 24 hr retention (in mg) and concentration (as integral sample/day, parts/10*) of TRI, and av urine level (mg/liter) and daily output (in mg) of TCA were measured. The amount of TRI retained daily was compared with the am ount of TCA excreted per day in the urine. The integrated TRI was compared with the av amount of TCA excreted. These values were calculated for the first three work days of the week (Monday. Tuesday, and Wednesday) and compared with each other. There appeared to be an individual rather than a general relationship between TRI and TCA. The ratio of TRI retention levels to TCA excretion values tended to increase during the week, with peak excretion 24-48 hr after exposure. The integrated TRI to av TCA excretion ratio varied between 1: 1.32 and 1:2.63. Urinary levels of metabolites of TRI may be a reliable index of exposure of industrial workers to TRI. (28 Refs) 76. EVIDENCE FOR EXISTENCE IN HUMAN TISSUES OF MONOMERS FOR PLASTICS AND RUBBER MANUFACTURE. WolfT MS Environmental Sciences Lab., Dept Community Medicine, Mount Sinai Sch. Medicine, City Univ. New York, New York. NY 10029 Environ Health Perspect; 17:183-187 1976 Discussion is made of the storage in fat of lipophilic substances such as DDT, polychlorinated biphenyl^, tetrachloroethylcne, and trichloroethylene, which are chemically similar to many industrially-used monomers, plus styrene, acrylamide, and vinyl chloride. Data on the uptake, metabolism, storage, and excretion of these substances in humans are provided when available. The storage and removal of lipophilic substances from fat tissues depend on fat so lubility, volatility, and metabolism. Styrene is very soluble in blood and fat; following exposure at 100 ppm, blood levels of 0.2-15 ppm styrene have been determined. The urinary and breath half-lives of styrene metabolites and styrene, respectively, are about 8 hr and 1-3 hr, respectively. Detectable levels of styrene were present in the fat of styrene workers greater than 2 days following exposure, a much longer period than that over which styrene could be detected in the breath of people experimentally exposed to 100 ppm styrene for sustained periods. (28 Refs) III. BIOLOGICAL EFFECTS IN BACTERIAL SYSTEMS, DROSOPHILA, AND EXPERIMENTAL ANIMALS A. Acute and Subacute Toxicity of Vinyl Chloride and Related Compounds in Experimental Animals 77. EFFECTS OF POLYVINYL CHLORIDE INGESTION BY DOGS. Johnson WS, Schmidt RE Operating Location AA, US Air Force Occupational Environmental Health Lab., Kelly Air Force Base, TX Am J Vet Res; 38(11); 1891-1892 1977 Polyvinyl chloride (PVC) acrylic thermoplastic sheeting (0.125 g/kg bid for 5 days) was fed to 6 dogs to determine whether ingestion during periods of normal transit of military working dogs would be toxic and thus affect the safety of this material for construction of shipping containers. The test material passed through the digestive system of all animals unchanged and hematologic examination, blood chemical analyses, and urinalyses were within normal limits. Gross and microscopic lesions attributable to the test material were not seen in necropsied animals 10 days after the feeding was stop ped. (9 Refs) 78. CHRONIC TOXICITY AND REPRODUCTION STUDIES OF HEXACHLOROBUTADIENE IN RATS. Kociba RJ, Schwetz BA, Keyes DG, Jersey GC, Ballard JJ, Dittenber DA, Quast JF, Wade CE, Humiston CG Toxicology Res. Lab., Health and Environmental Res., Dow Chemical Co., Midland, MI, 48640 Environ Health Perspect; 21:49-53 1977 The carcinogenic, reproductive, and toxic effects of po hexachlorobutadiene (HCBD), an unwanted by-product of processes associated with the chlorination of hydrocarbons, were assessed in Sprague Dawley rats. The LD50 values fol lowing a single po dose ranged from 504 to 667 mg/kg in adult male rats and from 200 to 400 mg/kg in adult female rats. In 21-day-old male and female weanling rats, the LD50 values were 46-91 and 26-81 mg/kg, respectively. In a chronic toxicity study, the ingestion of 20 mg/kg/day HCBD for up to 2 yr caused multiple toxicologic effects, primarily of the kidney. These included the development of renal tubular adenomas and adenocarcinomas. The ingestion of 2 mg/kg/day HCBD caused lesser toxicity and no evidence of neoplasia. No toxic effects were observed in rats chronically exposed to a po dose of 0.2 mg/kg/day. Thus, a dose-response relationship was observed for HCBD-induced toxicity.which affected primarily the kidney; HCBD-induced neoplasms occurred only at a dose level higher than that causing discernible renal injury. In a reproduction study, 20 or 2.0 mg/kg/day HCBD induced sl- 17 UCC 106340 ight maternal toxicity (primarily of the kidney),but caused no adverse effects on reproductive parameters (percent pregnancy and neonatal survival/development) A decreased neonatal body wt was noted at 20 mg/kg/day. No toxicologic effects were observed among adult rats at 0.2 mg/kg/day or among neonates at 0.2 or 2.0 mg/kg/day. chloride monomer (VCM) was determined in male Holtzman rats pretreated with Aroclor 1254. All compounds except ethane caused degeneration and necrosis of the liver. Ethylene, VFM, and VBM should be evaluated for chronic effects in view of their similarity of acute action to VCM, a known carcinogen. (20 Refs) 79. SHORT-TERM TOXICITY AND REPRODUCTION STUDIES IN RATS WITH HEXACHLORO-< 1,3)BUTADIENE. Har(eman JH, Seinen W Dept. Pathology and Hygiene, Coll. Veterinary Medicine, Univ. Illinois at Urbana-Champaign, Urbana, IL, 61801 Toxicol Appl Pharmacol; 47(1):1-I4 1979 Wistar-derived rats were given 0.4-15.6 mg/kg/day hexachloro-(l,3)-butadiene (HCBD) by gavage for 13 wk, or 150 or 450 ppm in the diet for 2 wk. The main toxic action of HCBD was in the kidneys. Decreased urine-concentrating ability was found in females at low doses, and extensive tubular degeneration, degeneration and necrosis of individual epithelial cells, plus increased cellularity of the epithelial lining were noted in both sexes at high doses. There were no significant effects on fertility or progeny. (24 Refs) 80. SATURABLE METABOLISM AND THE ACUTE TOXICITY OF 1,1-DICHLOROETHYLENE. Andersen ME, French JE, Gargas ML, Jones RA, Jenkins LJ Naval Medical Res. Inst. Toxicology Detachment (NMRI/TD), Wright-Patterson AFB, OH. 45433 Toxicol Appl Pharmacol; 47(2):385-393 1979 The saturable metabolism and the acute toxicity of 1,1dichloroethylene (1,1-DCE) were studied. Concentration of and duration of exposure were examined as determinants of the inhalation toxicity of 1,1-DCE in fasted male rats of various ages. Phenobarbital sleep times (PBST) were significantly increased after exposures as brief as 0.5 hr in im mature rats (100-150 g) exposed to 200 ppm 1,1-DCE for durations up. to 2 hr; extensive dilation and vacuolization of the endoplasmic reticulum were also observed following 1,1DCE exposure. Older rats (greater than 200 g) were less susceptible to 1,1-DCE than were younger rats. PBST was not increased during exposure of older rats. Groups of six older rats were exposed to various concentrations of 1,1-DCE for 4 hr and observed for mortality. The concentration-mortality curve increased rapidly between 100 and 200 ppm, but maintained a plateau as concentration was increased between 200 and 1,000 ppm. The dependence of LT50 (the time of exposure required to kill half an exposed group at a given concentration) on concentration was not very great. A 1-fold increase in concentration from 200 to 2,000 ppm decreased the LT50 by less than a factor of 3. It is apparent that, even in this narrow range of doses, a concentration x time relationship is not valid. This is further evidence that a saturable, enzymatic activation occurs in the expression of the toxicity of 1,1-DCE. (19 Refs) 81. ACUTE HEPATOTOXICITY OF ETHYLENE, VINYL FLUORIDE, VINYL CHLORIDE, AND VINYL BROMIDE AFTER AROCLOR 1254 PRETREATMENT. Conolly RB, Jaeger RJ, Szabo S Dept. Physiology, Harvard Sch. Public Health, Boston, MA 02115 Exp Mol Pathol; 28<l):25-33 1978 The hepatotoxicity of ethane, vinyl ethylene, fluoride monomer (VFM), vinyl bromide monomer (VBM), and vinyl 82. TOXICITY OF BETA-CHLOROPRENE(2CHLOROBUTADIENE-1,3): ACUTE AND SUBACUTE TOXICITY. Clary JJ, Feron VJ, Reuzel PG Environmental and Health Affairs, Celanese Corporation, I2U, Avenue of the Americas, New York, NY, 10036 Toxicol Appl Pharmacol; 46(2);375-384 1978 The acute and subacute toxicity of beta-chloroprene (2chlorobutadiene-1,3) was evaluated using acute inhalation toxicology, the Class B poison test, and a 4-wk range study. The approximate lethal concentration (ALC) was determined by exposing (inhalation) groups of six male albino rats to concentrations of beta-chloroprene ranging from 1.95 to 13.30 mg/liter for 4 hr; the rats were weighed daily for 14 days and then submitted for histological examination. A total of five rats died following inhalation of beta-chloroprene (1/6 at concentration of 8.42 mg/liter and 2/6 each at concentrations of 13.04 and 13.30 mg/liter; ALC = 8.42 mg/liter). Examination of the rats following exposure showed hem orrhagic infarction of the lungs and central and mid-central necrosis of the liver. Forty male and 40 female Specific Pathogen-Free rats were equally divided into four groups and exposed to beta-chloroprene concentrations ofO, 39, 161, and 625 ppm for 6 hr/day, 5 days/wk for 4 wk. Eye irritation, restlessness, lethargy, nasal discharge, and oranged-colored urine were observed in animals exposed to the higher concentrations. Hair loss was observed in several female rats. The cumulative mortality was 5/10 males and 3/10 females at 625 ppm and 3/10 males and no females at 161 ppm. No rats at the lower exposure rates. In a similar experiment using hamsters, all the animals exposed to 630 ppm and 1/10 males and 3/10 females exposed to 162 ppm died during the first wk. Significant differences in relative organ wt between test and control animals were observed in all organs except the heart. The av body wt of both male and female rats tested decreased with increasing concentration of beta-chloroprene. It was concluded that beta-chloroprene is not a Class B poison. (19 Refs) 83. THE TOXICITY OF SOME HALOMETHANES IN MICE. Bowman FJ, Borzelleca JF, Munson AE Dept. Pharmacology, Medical Coll. Virginia, Richmond, VA, 23298 Toxicol Appl Pharmacol; 44(1)*.213*2I5 1978 The toxicity of some halomethanes (trichloromethane, bromodichloromethane, dibromochloromethane, and tribromomethane) was tested in mice as part of an extensive toxicological evaluation designed to identify potential risk to humans. Trichloromethane and tribromomethane were the least toxic with LD50 doses above 1000 mg/kg in both sexes. Males were more sensitive than females to the lethal effects of the compounds. Necropsies performed on animals showed fatty liver infiltration; evidence of hemorrhaging was observed in the adrenals, lungs, and brain. Other tissues showed no gross pathological changes. (6 Refs) 18 ucc 106341 i B. Morphologic and Biochemical Changes in Experimental Animals Exposed to Vinyl Chloride and Related Compounds The reader may also find the following abstracts of interest: 124. 125. 244 84. EFFECTS OF VINYL CHLORIDE EXPOSURES TO RATS PRETREATED WITH PHENOBARBETAL. Drew RT, Harper C, Gupta BN, Talley FA Nall. Inst, Environmental Health Sciences. P.O. Box 12233, Res. Triangle Park, N.C. 27709 Environ Health Perspect; 11:235-242 1975 Male Charles River CD-I rats were exposed to ten consecutive days. 6 hours/day, to vinyl chloride vapors at an average concentration of 13,500 ppm. The exposed rats were divided into three groups of eight rats each: each group was pretreated with 3- methylcholanthrene ; (15 mg/kg, ip for two days prior to first exposure); one group was pretreated with phenobarbital (I mg/ml in drinking water for three days prior to exposure and during exposure); and the third group received no treatment. Half the animals in each group were sacrificed 18 hr after the last exposure and half were sacrificed four days later, in a second experiment, four rats pretreated with phenobarbital were exposed to vinyl chloride vapors at a concentration of 17,300 ppm for two days and sacrificed on the third day. In both experiments control animals, also treated with phenobarbital or 3-methylchoIanthrene, were exposed to air only. At the time of sacrifice, lungs, kidneys, spleen, heart, and a small piece of the liver from each animal were preserved for histological examination. The remainder of the liver was processed for assay of microsomal enzyme activity. The following parameters were investigated; growth rate, organ weights, morphological changes, and both benzplietamine-N-demethylase activity and cytochrome P-450 content of microsomes prepared from the livers. In both experiments the only marked difference noted in any group was a decrease in the growth rate of the animals exposed to vinyl chloride and treated with phenobarbital. This decreased growth rate was particularly apparent on the third day of the vinyl chloride exposures. Occasional morphological changes were also seen in the livers of the animals treated with phenobarbital and exposed to vinyl chloride. Although changes resulting from exposure to VC and phenobarbital have been demonstrated, these changes have not been shown to be preneoplastic. 85. ACUTE HEPATIC INJURY BY VINYL CHLORIDE IN RATS PRETREATED WITH PHENOBARBITAL. Jaeger RJ, Reynolds ES, Conolly RB, Moslen MT, Szabo S, Murphy SD Kresge Cent. Environ. Health, Harvard Sch. Public Health, Boston, Mass. Nature (Lond); 252(5485):724-726 1974 The potential of vinyl chloride monomer to enhance liver injury in male Holtzman rats pretreated with phenobarbital. an inducer of certain enzymes of the liver mixed-function oxidase system, was studied. Four experimental groups were defined: exposed to air and not pretreated; exposed to vinyl chloride monomer and not pretreated; exposed to air and pretreated with phenobarbital (0.1% in drinking water for 7 days); and exposed to vinyl chloride monomer and pretreated with phenobarbital. Nonpretreated and phenobarbitalpretreated rats exposed to air alone had no increase in serum alanine-alpha-ketoglutarate transaminase (AKT) and sorbitol dehydrogenase, enzymes that are highly specific for liver injury. In nonpretreated groups, exposure to 0.5% or S% vinyl chloride monomer for a single six hour period did not cause a substantial rise in serum alanine-alpha-ketoglutarate transaminase or sorbitol dehydrogenase, but after exposure to 10% vinyl chloride monomer there was a slight increase in these enzymes. Histologically, centrolobular hepatocellular vacuolization was noted only in the 10% exposure group. Phenobarbital pretreatment for seven days caused a marked enhancement of injury at the 5% level of exposure, with vacuolization of centrolobular parenchymal cells, focal necrosis of mid-zonal parenchyma, dilation of the rough endoplasmic reticulum, and formation of tubular snarls by smooth endoplasmic reticulum. Phenobarbital-pretreated rats killed after the last exposure to 5% vinyl chloride monomer following 5 consecutive days of exposure did not have increased activities of serum alanine-alpha-ketoglutarate transaminase or sorbitol dehydrogenase, suggesting that previous exposure to vinyl chloride monomer blocked the biochemical response which followed reexposure at short intervals. 86. BIOLOGICAL EFFECTS OF VINYL CHLORIDE: AN EXPERIMENTAL STUDY. Winell M, Holmberg B, Kronevi T Section Occupational Toxicology, Dept. Occupational Medicine, Natl. Board Occupational Safety and Health, S-100 26 Stockholm. Sweden Environ Health Perspect; 17:211-216 1976 Liver damage caused by the exposure of albino NMRI mice to atmospheric vinyl chloride (VC) was assessed by estimating the plasma activities of alkaline phosphatase (AP), the transaminases, and lactate dehydrogenase (LDH). Three groups of 24 mice each were exposed by inhalation for 6 hr/day, 5 days/wk, to 50 ppm (52 wk) or 5(W ppm VC (26 wk), or to air only (controls). The animals were also autopsied, and the tissue pathology was studied. Liver damage was indicated by a significant increase in total LDH levels after about 40 wk. After 46 wk, total LDH levels were increased about 2.5-fold following exposure to 500 ppm. A significant shift in the LDH isoenzyme profile to the M form also occurred. There was no corresponding elevation in transaminase activities, which might have served as an alternative indication of liver injury. AP activities also increased after about 40 wk: at this time levels were elevated 30%-40% and 50%-60% following exposure to 50 and 500 ppm VC, respectively. This elevation could indicate lesions in the hepatobiliary tract. Upon autopsy 12 mo after the start of exposure, no control mice had any lung adenomas or hemangiosarcomas, and 24 mice exposed to 24 ppm VC had lung adenomas and 8 had hemangiosarcomas. (30 Refs) 87. SOME ASPECTS ON DOSE-RESPONSE IN VINYLCHLORIDE-INDUCED LIVER INJURY AND TUMORS IN MICE (MEETING ABSTRACT). Holmberg B, Kronevi T, Winell M Section Occupational Toxicology, Natl. Board Occupational Safety and Health, Stockholm, Sweden Scand J Clin Lab Invest; 37(Suppll47):74 1977 Vinyl chloride monomer (VCM) induces liver injury and liver hcmangiosarcoma in PVC workmen and is also carcinogenic in rodents. Mice were exposed by inhalation to 50 and 500 ppm VCM during 12 and 6 mo respectively. Blood samples were taken every 6th wk for analysis of glutamic pyruvic transaminase (CPT), glutamic oxalacetic transaminase (GOT), acid phosphatase (AP), and total lactic dehydrogenase (LDH) as well as LDH isoenzymes. Some mice were sacrificed for histopathological examinations after 6 mo and the rest when dead or moribund. AP and total LDH activities were elevated in VCM exposed mice. The percentage of M form was also increased in exposed animals. The transaminases were not elevated. Enzyme activities were, however, increased after the appearance of tumors. A 19 UCC 106342 tendency to a dose-dependency was observed in total LDH activities as well as in the frequency of tumor-bearing animals. The significance of the data will be discussed with reference to possible early detectability of VCM-induced tissue injury and with reference to current knowledge of dose-response relationships for chemically induced tumors. 88. INITIAL FEATURES OF VINYL CHLORIDE (VC) HEPATIC INJURY (MEETING ABSTRACT). Schaffner F, Popper H, Selikoff IJ Mount Sinai Sch. Medicine, City Univ. New York, New York, NY 10029 Gastroenterology; 71 (5):A35/928 1976 An attempt was made to define the vinyl chloride (VC)induced lesions in the livers of mice after exposure to gaseous VC 5 hr/day, 5 days/wk for 1,3 and 6 mo at 2,500 and 6,000 ppm. Animals were also studied I mo after exposure ceased. Hepatocellular changes seen as early as I mo included hy pertrophy of the [smooth endoplasmic reticulum, reflecting metabolism of VC and plasma membrane loss of microvilli and invagination. These findings reflected a movement of a possible injurious metabolite across the membrane. The sinusoidal reaction was multicellular. The size and number of lipocytes was increased with little fibrosis. These cells ap peared normal except during recovery, when their fat content decreased in a patchy fashion. Macrophages were large and fil led with phagosomes, some containing long needle-like crystals. Lymphocytes were numerous, but no plasma cells were seen. The main abnormality involved the endothelial lining cells. Early changes resembled swollen cells; later, the bulky, and, in places, multilayered cells contained more organelles, especially mitochondria and endoplasmic reticulum (ER) but no phagosomes. Discontinuities developed in the sinusoidal walls so that RBC were not only in but also around sinusoids dilated by beginning peliosis hepatis. Platelet thrombi were also seen in and around sinusoids. The lining cel ls, probably the precursors of angiosarcoma, resembled fibroblasts but nowhere did their ER contain fluffy collagen components. These observations suggest that VC is metaboliz ed in hepatocytes, and that metabolites leave these cells through the plasma membrane to injure sinusoidal lining cells, eventually producing angiosarcomas. Attempts at screening for VC hepatic injury should be directed at endothelial cells and altered microcirculation rather than at hepatocytes, macrophages or fibroblasts, (no refs) 89. ELEVATED GLUTATHIONE CONTENT, GLUTATHIONE-S-TRANSFERASE AND GLUTATHIONE REDUCTASE IN LIVER OF RATS EXPOSED TO VINYL CHLORIDE (MEETING ABSTRACT). Du JT, Tamburro CH Digestion, Disease and Nutrition Section, Dept. Medicine, Cancer Center, Univ. Louisville Medical Sch., Louisville, KY 40232 Fed Proc; 37(6); 1545 1978 Vinyl chloride (VC) is believed to be metabolized to chloroethylene oxide (CEO) and chloroacetaldehyde, and detoxified by way of glutathione. Rats were exposed to 28,000 ppm VC, 7 hr/day, 5 days/wk for a 4 and 6 wk, the activity of glutathione epoxide-S-transferase (GEST) was elevated 30 to 54% over normal control and air control (9.71 +* 0.68 vs 7.52 +- 0.97 and 6.30 +* 0.68) respectively. However, the activity of glutathione aralkyl-S-transferase (GAST) was not significantly elevated until 6 wk of exposure to VC. The content of reduced glutathione was also elevated 45% in the VC treated group and the activity of the glutathione reductase, the enzyme to regenerate glutathione from the oxidized form was elevated 50%. These results demonstrate that VC exposed rats have the capacity to maintain glutathione reductase activity and glutathione concentration for detoxification. Further, it sug gests that the primary route of VC metabolism is initial oxidation to CEO and then detoxification by GEST directly. With longer exposure and probable saturation of the direct route, there is greater rearrangement of CEO to chloroacetaldehyde, and detoxification with glutathione as supported by the delayed induction of GAST. 90. THE EFFECT OF VINYL CHLORIDE MONOMER, CHLOROETHYLENE OXIDE AND CHLORACETALDEHYDE ON DNA SYNTHESIS IN REGENERATING RAT LIVER. Border EA, Webster L Natl. Res. Inst. Occupational Diseases, S. African Medical Res. Council, P O Box 4788, Johannesburg, 2000 S. Africa Chem Biol Interact; 17(2):239-247 1977 A study was made of the effects of vinyl chloride monomer (VCM) and two of its presumed metabolites, chloracetaldehyde (CA) and chloroethylene oxide (CEO), on DNA synthesis in the regenerating livers of 100-g Wistar rats subjected to partial hepatectomy. VCM (0.5 ml of a 0.19% so lution) injected iv 30 min after partial hepatectomy reduced the first ensuing wave of DNA synthesis (at 21 hr) by about 50%; no effect on the second wave of DNA synthesis (at 30 hr) was evident. Similar treatment with CEO and CA also depressed the first wave of DNA synthesis by about 50%. However, these substances had different effects on the second wave; CEO raised the rate of DNA synthesis by about 50%, but CA tended to desynchronize the normally well-defined second wave. It is concluded that VCM, CEO, and CA have similar effects as unrelated carcinogens in retarding DNA replication, (37 Refs) 91. VINYL CHLORIDE-INDUCED DEPRESSION OF HEPATIC NON-PROTEIN SULFHYDRYL CONTENT AND EFFECTS ON BROMOSULPHALE1N (BSP) CLEARANCE IN RATS. Watanabe PG, Hefner RE, Gehring PJ Toxicology Res. Lab., Health and Environmental Res. 1803 Building, Dow Chemical Company, Midland, Ml 48640 Toxicology; 6(1); 1-8 1976 The effects of acute inhalation exposure to vinyl chloride on the non-protein sulfhydryl content in male Sprague-Dawley rat liver were studied. The rats were exposed to 2,000, 1,000, 250, 150, 50, or 10 ppm. Exposure to 2,000 ppm resulted in a progressive depression of hepatic non-protein sulfhydryl content reaching 33% within 2 hr. 47% after 4 hr, and 62% after 7 hr. An apparent max depression was observed after 4 to 5 hr of exposure to vinyl chloride at 1,000, 250 or 50 ppm. Depression after 7 hr exposure to 50 ppm was inconsistent. Exposure to 1,000 ppm vinyl chloride did not alter the serum clearance of bromosulphalein. (25 Refs) 92. COMPARISON OF THE FATE OF VINYL CHLORIDE FOLLOWING SINGLE AND REPEATED EXPOSURE IN RATS. Watanabe PG, Zempel JA, Gehring PJ Toxicology Res. Lab., Health and Environmental Res., Dow Chemical USA, Midland, MI, 48640 Toxicol Appl Pharmacol; 44(2):39l*399 1978 The metabolic fate of vinyl chloride (VC) was compared in rats exposed by inhalation once or repeatedly. The activities of the microsomal enzymes (aniline hydroxylase and pnitroanisole-O-demethylase) was essentially the same in both groups as well as nonexposed control rats. Repeated exposure to VC did not induce its biotransformation since covalent bonding to hepatic macromolecules was greater in rats repeatedly exposed. (17 Refs) 20 UCC 106343 93. SOME BIOCHEMICAL AND HISTOPATHOLOGICAL CHANGES INDUCED BV POLYVINYL CHLORIDE DUST IN RAT LUNG. Agarwal DK, Raw JL, Srivastava SP, Seth PK Industrial Toxicology Res. Centre, Mahatma Gandhi Marg. Post Box No. 80, Lucknow-226001, India Environ Res; 16(l/3):333-341 1978 Enzymatic and pathomorphologic alterations in rat lungs were studied at different time intervals up to 180 days after a single intratracheal administration of 25 mg of polyvinyl chloride dust. The activities of two energy-linked enzymes, succinic dehydrogenase (SDH) and adenosine triphosphatase (ATPase), and three lysosomal enzymes, acid phosphatase, beta-glucuronidase, and ribonuclease, were significantly increased in the early period and then started to decline. The activities of SDH and ATPase reached control values at 150 days, while those of the lysosomal enzymes remained significantly higher up to this period. Histopathologically, the pulmonary response was in the form of acute inflammatory changes during the early stages of dust burden, followed by the development of granulomatous lesions containing small am ounts of stromal elements. (Author abstract) (32 Refs) 94. VINYLIDENE CHLORIDE-INDUCED ULTRASTRUCTURAL CHANGES IN RAT LIVER (MEETING ABSTRACT). French JE, Andersen ME, Jenkins LJ Experimental Pathology Dept., AFRRI-NNMC, Bethesda, MS 20014 J Cell Biol; 70(2/Part2):361a 1976 Vinyl chloride and vinylidene chloride( 1,1dichloroethylene, DCE) are closely related chemicals and are known occupational and environmental contaminants. The pathobiological effects after oral administration of DCE (in corn oil) to fasted male Holtzman rats was determined by correlating the ultrastructural damage to the liver with changes in the serum levels of glutamic-pyruvic transaminase (SGPT) and glutamic-oxaloacetic transaminase (SGOT). In a completely randomized study, fasted rats received a single oral dose of 40 to 80 mg DCE/mg of body wt. After 0,1.2,4, and 8 hr, SGPT and SGOT values were determined and liver tissue samples were immersion-fixed in 3% cacodylate buffered glutaraldehyde, post-fixed in osmium tetroxide and prepared by conventional EM methods. SGPT and SGOT values increased dramatically according to dosage and time of exposure in a linear manner, which indicated significant hepatic damage. The appearance of myelin-like bodies occurred at a similar frequency in both control and treated rats. Control rats were also characterized by slightly dilated rough endoplasmic reticulum(RER) and perinuclear cistcrnae. However, DCE-cxposed rats showed significant dilation of the RER, loss of ribosomes, mitochondrial swelling, loss of cristae and chromatinolysis in a dose- and time-related manner. Margi nation of the chromatin along the nuclear envelope (chromatinorrhexis) did not occur within this time period of exposure and dosage of DCE. DCE is a very hepatoxic xenobiotic and its pathobiology may be different according to the route of administration. (No refs) 95. TRICHLOROETHYLENE-INDUCED DEACTIVATION OF CYTOCHROME P-450 AND LOSS OF LIVER GLUTATHIONE IN VIVO. Moslen MT, Reynolds ES, Boor PJ, Bailey K.. Szabo S Department of Pathology, The University of Texas Medical Branch, Galveston, Texas 77550 ResCommun Chem Pathol Pharmacol; 16(1):109-120 1977 Liver microsomal enzyme activities and glutathione (GSH) contents of fasted male rats pretreated with phenobarbital (PBT) or vehicle controls were measured during and after exposure to trichloroethylene (TRI) (1% x 2 hr). TRl caused morphologic liver injury only in the PBT animals. Cytochrome P-450 and b5 contents were diminished by the end of the first hr of TRI exposure and NADH-cytochrome c reduction increased three-fold by eight hr in the PBT animals. The only change in vehicle animals exposed to TRI was a decrease in NADPH-cytochrome c reductase activity by eight hr. Hepatic GSH contents of vehicle animals, constant during TRI exposure, rose with time. In contrast, in PBT animals, hepatic GSH contents decreased during TRI exposure and then rebounded. Decreases in GSH were most profound in the microsomal fraction. When fed animals with approximately equal to two-fold higher hepatic GSH levels than fasted animals were exposed to TRI, they had shorter anesthesia rec overy times and less liver injury, although excreting similar or slightly more trichlorinated metabolite into their urine in 24 hr than their fasted counterparts. We suggest that the hepatotoxic effects of trichloroethylene are caused by inadequate detoxification of its reactive intermediates. (Author Abstract) 96. ACUTE TRICHLOROETHYLENE HEPATOTOXICITY AND INDUCTION OF MICROSOMAL ENZYMES (MEETING ABSTRACT). Moslen MT, Reynolds ES, Szabo S Peter Bent Brigham Hosp., Boston, MA Am J Pathol; 82(2): 36a-37a 1976 To determine the effects of differential induction of mixed-function oxidase system components on the metabolism and hepatotoxicity of trichloroethylene, male rats were pretreated with isomolar doses (400 micromoles/kg/day) of phenobarbital (PBT), 3-methylcholanthrene (3-MC), hexachlorobenzene (HCB), pregnenolone-16alphacarbonitrile (PCN) or spironolactone (SNL) or 150 micromoles/kg of Aroclor 1254 (A-1254) for 7 days be gavage. The animals were then exposed to trichloroethylene (1% in air for 2 hr) and sacrificed. Liver injury 24 hr after exposure was most severe in animals treated with PBT or A-1254. Hepatocellular injury was predominantly centrilobular in PBT-treated animals and periportal in A-1254-treated animals. SGOT elevations, morphologic injury and/or electrolyte change were of lesser extent 24 hr after exposure in 3-MC, HCB and PCN groups; changes were not apparent in the SNL group. Liver injury as quantitated by mean SGOT was related to the magnitude of induction of cytochrome P450 and to a lesser extent to NADPH-cytochrome P-450 reductase. Enhanced urinary excretion of tricholorethylene metabolites was also correlated with P-450 content. (0 Refs) 97. TRICHLOROETHYLENE-INDUCED DEACTIVATION OF LIVER ENDOPLASMIC RETICULUM AND GLUTATHIONE DEPLETION (MEETING ABSTRACT). Reynolds ES, Moslen MT, Boor PJ, Bailey K. Szabo S Dept. Pathology, Univ. Texas Medical Branch, Galveston, TX Toxicol Appl Pharmacol; 41(1):217 1977 We have undertaken further studies to monitor mixedfunction oxidase system activities and liver glutathione (GSH) contents during the development of trichloroethylene (TRI>induced injury in phenobarbital (PBT)*pretrcated animals. Male Charles River rats weighing 200 g were given PBT (400 micromole/kg) or vehicle po for 7 days. On the morning of day 8, after an overnight fast, animals were exposed to air or to 1% TRI for 2 hr. In the PBT-TRI group, contents of cytochrome P450 and cytochrome b5 diminished by the end of the 1st hr of TRI exposure and NADH-cytochrome c reduction increased three-fold by the 8th hr. In contrast, the only change in the vehicle-TRl group by 8 hr was decreased NADPH-cytochrome c reduction. Hepatic GSH contents of vehicle-TRl animals were constant during TRI exposure but 21 UCC 106344 then rose almost twofold by 12 hr. Hepatic GSH contents of PBT-TRI animals decreased during exposure and then reb ounded; decreases were most profound in the microsomal fraction. Because of this apparent involvement of GSH in TRl's biotransformation, we also exposed fed animals with approx 2 x higher hepatic GSH concentrations to TRI. While 24 hr urinary metabolite excretions were similar, the fed animals had shorter anesthesia recovery times and less liver injury. The hepatotoxicity of TRI appears to be caused by inadequate rates of detoxification of its reactive intermediates, (no Refs) 98. LIVER ENDOPLASMIC RETICULUM: TARGET SITE OF HALOCARBON METABOLITES. Reynolds ES Dept. Pathology, Peter Bent Brigham Hosp,, Boston, MA 02115 Adv Exp Med Biol; 84:117-137 1977 The hepatotoxic effects of carbon tetrachloride, vinyl chloride, trichloroethylene, and halothane are reviewed. Initial injury produced by exposure to these chemicals involves the endoplasmic reticulum. There is dispersion of the ergastoplasm, then vacuolization and degranulation of the rough endoplasmic reticulum with concomitant retraction of the smooth endoplasmic reticulum into tightly clumped tubular aggregates. Membranes in these tubular aggregates seem to undergo supramolecular disassembly. This structural disorganization is accompanied by a diminished functional capacity in the organelle. Activation of these halocarbons to toxic species by the endoplasmic reticulum is indicated (1) by the enhancement of their toxicity upon pretreatment with chemicals, such as phenobarbital, or Aroclor 1254 that induce components of the mixed function oxidase system and (2) by the formation of certain metabolites and/or covalently bound products. It is not clear whether the halocarbons cause liver injury by the covalent binding of free-radical metabolites to tissue macromolecules or by initiating lipid pcrodixidation. (51 Refs) 99. BIOCHEMICAL AND TOXICOLOGICAL EFFECTS OF COMBINED EXPOSURE TO 1,1,1TRICHLOROETHANE AND TRICHLOROETHYLENE ON RAT LIVER AND BRAIN. Vainio H, Savolainen H, Pfaffli P Dept. Industrial Hygiene and Toxicology, Inst. Occupational Health, Haartmaninkatu 1, SF-00290 Helsinki 29, Finland Xenobiotica; 8(3); 191-196 1978 Male Wistar rats inhaled a mixture of 1,1,1trichloroethane (1,1,1-TCE) (500 ppm) and trichloroethylene (200 ppm) 6 hr/day for 4 days. As a result, 1,1,1-TCE accumulated in the perirenal fat, the hepatic RNA content doubled, hepatic glutathione concentrations decreased sl ightly, and uridine diphosphatc-glucuronosyl transferase activity doubled. A 5-day exposure increased the content of both solvents in various organt rapidly, depressed brain RNA, and decreased in styrene monooxygenase activity. Changes noted on day 5 had also occurred on days 1-4 but had reverted during each postexposure period. (22 Refs) 100. DAMAGE TO HEPATIC CELLULAR MEMBRANES BY CHLORINATED OLEFINS WITH EMPHASIS ON SYNERGISM AND ANTAGONISM. Reynolds ES, Moslen MT Dept. Pathology, IJniv. Texas Medical Branch, Galveston, TX, 77550, 77550 Environ Health Perspect; 21:137-147 1978 The biochemical mechanisms responsible for the toxicity of vinyl chloride (VC), 1,1-dichlorocthylene (1,1-DCE), trichloroethylene (TCE), and perchloroethylene (PCE) were investigated in male Sprague-Dawley rats pretreated with phenobarbital, 3-methylcholanthrene, hexachlorobenzene, spironolactone, or pregnenolone-l6alpha-carbonitrile. The most nonsymmetrically depolarized compound, 1,1-DCE, was the most hepatotoxic and caused a unique pattern of hepatocellular injury involving mitochondria, plasma mem branes, and chromatin. The injury induced by the other chloroethylenes appeared to affect the structural integrity of the endoplasmic reticulum profoundly, with the toxic potential in the following order: TCE greater than VC greater than PCE. Pretreatments that increased the cytochrome P-450 content (increased metabolic activation) enhanced or were synergistic to the hepatotoxic potential of TCE, VC, and PCE, but they were protective or antagonistic to 1,1-DCE hepatotoxicity. This suggests that the biologic response to 1,1DCE may be expressed by a different metabolic pathway. Glutathione appears to be involved in the biologic response to all nonsymmetric chloroethylenes and to act as an antagonist against injury. Marked differences in the patterns of injury and the biologic responses suggest that more than one mechanism is involved in the production of injury by chloroethylenes. (37 Refs) 101. AN EXPERIMENTAL APPROACH FOR EVALUATING GENETIC AND EPIGENETIC CONTRIBUTIONS TO CHEMICAL CARCINOGENESIS (MEETING ABSTRACT). Reitz RH, Schumann AM, Watanabe PG, Quast JF, Gehring PJ Toxicology Res,, Dow Chemical Co., Midland, Ml, 48640 Proc Am Assoc Cancer Res; 20:266 1979 Many chemical carcinogens appear to cause cancer because of their ability to react irreversibly with cellular DNA, producing mutations which transform normal cells into malignant clones (genotoxicity). However, cancer has also been produced in animals by chemicals which do not appear to react with DNA. suggesting that epigenetic mechanisms can also be important. Because the safety precautions appropriate to each type of agent arc quite different, we have attempted to evaluate the in vivo contribution from each type of mechanism with known animal carcinogens. Genetic effects are shown by dimethylnitrosamine (DMN). DNA isolated from the liver of treated rats (10 mg/kg) contains 0.3% alkylated bases and mice injected with 20 mg/kg DMN are carrying out 7.23 x as much DNA repair (hydroxyurea-resistant 3H-thymidine incorp oration) as controls. In contrast, animals exposed to 500 mg/kg perchloroethylene (PERC) showed no evidence of DNA alkylation (less than 0.001%) but did show elevated DNA turnover (2 x controls) and histopathology in the liver. In studies with other animal carcinogens, DNA turnover and epigenetic carcinogenesis have been correlated with species sensitivity, tumor and nontumor sites, and tumorigenic and nontumorgenic doses. Consequently it appears that the relative importance of the two mechanisms of carcinogenesis can be estimated after in vivo measurements of DNA damage, DNA repair, and DNA turnover. (1 Ref) 102. REPAIR OF RAT LIVER DNA IN VIVO DAMAGED BY ETHYLENE DIBROMIDE. Nachtomi E, Sarma DS Fels. Res. Inst., Dept. Pathology, Temple Univ. Sch. Medicine, Philadelphia, PA 19104 Biochem Pharmacol; 26(20); 1941-1945 1977 Tube feeding of UCethylenc dibromidc (EDB) to nonfasted Wistar rats resulted in the incorporation of the radioactivity into liver DNA, RNA, and protein. The pesticide caused slower sedimentation of liver DNA in alkaline but not in neutral sucrose gradients. The slower sedimentation of liver DNA in alkaline sucrose gradients was apparent within 2 hr after the administration of a dose of 22 mg/ lOOg body wt or 4 22 UCC 106345 hr after a dose of 7.5mg/ lOOg. The liver DNA damage induced by EDB at 7.5 mg/100 g was repaired significantly by 17.5 hr and almost completely by 96 hr. Administration of diethyldithiocarbamate, a free radical scavenger, did not inhibit liver DNA damage caused by EDB. The results indicate that EDB produces both chemical and physical lesions in liver DNA. (39 Refs) C. Mutagenicity of Vinyl Chloride and Related Compounds in Bacterial Systems, Drosophila, and Experimental Animals The reader may also find the following abstracts of interest.- 39. 68. 166. 196, 199. 200, 201, 324 103. MUTAGENICITY OF INDUSTRIAL COMPOUND: VINYL CHLORIDE, STYRENE AND THEIR POSSIBLE METABOLITES (MEETING ABSTRACT). (PP. 175-176) Loprieno N, Abbondandolo A, Barale R, Baroncelli S, Bonatti S. Bronzetti G, Cammellini A, Corsi C, Corti G, Frezza D, Leporini C, Mazzaccaro A, Nieri R, Rosellini D, Rossi AM Laboratorio di Mutagenesi e Differenziamento, CNR e Istituto di Genetica della Universita, Pisa, Italy Third International Symposium On Detection And Prevention Of Cancer. 1976. It has been proposed that mutagenicity tests are at the present the most appropriate for the prescreening of substances for possible carcinogenic activity. It is therefore interesting to develop biological analyses to assess the mutagenic activity of toxic industrial compounds already known as human carcinogens or those compounds under suspicion. In our analyses we .have applied mutagenicity methodologies in the study of vinyl chloride (human carcinogen) and to styrene (under carcinogenic analysis at the present): the same analyses have been applied also to their possible metabolites (2-chloroethylene oxide, 2-chloroethanol, 2-chloroacetaldehyde, and styrene oxide) in order to correlate the mammalian metabolic fate of the compounds with their biological activity. The compounds have been studied by means of liver microsomal assay and host-mediated assay (mice), employing as a test organism the yeast S pom be and S. cerevisiae on which the induction of gene-mutations and of gene-conversions has been analyzed. Preliminary experiments have been done also with somatic mammalian cells (V79 Chinese hamster), on which the induction of 8-azaguanine resistant clones has been assessed: From our analyses it has been found that vinyl chloride is mutagenic in the presence of liver microsomal preparations (in vitro) or in the hostmediated assay (in vivo). (Author Abstract) 104. MUTAGENICITY OF INDUSTRIAL COMPOUNDS: VINYL CHLORIDE, STYRENE AND THEIR POSSIBLE METABOLITES (MEETING ABSTRACT). Loprieno N, Abbondandolo A, Barale R, Baroncelli S, Bonatti S. Bronzetti G, Cammellini A, Corsi C. Corti G, Frezza D, Leporini C, Mazzaccaro A, Nieri R, Rosellini D, Rossi A Laboratorio di Mutagenesi e Differenziamento, CNR e Istituto di Genetica dell'Universita, Pisa, Italy Mutat Res; 38(2): 114-115 1976 Mutagenicity methodologies were applied to the study of vinyl chloride, styrene, and their metabolites (2-chloroethylcne oxide, 2-chloroethanol, 2-chloroacetaldchyde, and styreije oxide) to correlate the mammalian metabolic fate of the comp ounds with their biological activity. The compounds were studied by liver microsomal assay and host-mediated assay with the yeast S pom be and S cerevisiae. Preliminary experiments were also done with somatic mammalian cells (V79 Chinese hamster), on which the induction of 8-azaguanme resistant clones was assessed. Vinyl chloride was found to be mutagenic in the presence of liver microsomal preparations and in the host-mediated assay; moreover, the possible in vivo metabolite, 2-chloroethylene oxide, was responsible for mutagenic activity. Styrene was found to be inactive on yeast (+- microsomes) or slightly active on hamster cells; styrene oxide was found to be active in different biological systems. (No refs) 105. A DOMINANT LETHAL STUDY IN MALE RATS AFTER REPEATED EXPOSURES TO VINYL CHLORIDE OR VINYLIDENE CHLORIDE. Short RD, Minor JL, Winston JM, Lee CC Pharmacology and Toxicology, Midwest Res. Inst., 425 Volker Blvd., Kansas City, MO, 64110 J Toxicol Environ Health; 3(5/6):965-968 1977 Germinal mutations, as manifested by a dominant lethal effect, in male rats exposed to 0, 50, 250, or 1000 ppm vinyl chloride or 55 ppm vinylidene chloride (6 hr/day for 5 days/wk) were studied. The males were mated with untreated females after II wk of exposure. No evidence of pre- or postimplantation loss in the pregnant females was observed. (9 Refs) 106. VINYL CHLORIDE: DOMINANT LETHAL STUDIES IN MALE CD-I MICE. Anderson D, Hodge MC, Purchase IF Imperial Chemical Industries Ltd., Central Toxicology Lab., Alderley Park, North Macclesfield, Cheshire SK10 4TJ, England Mutat Res ; 40(4):359-370 1976 The mutagenic activity of vinyl chloride (VC) was investigated in CD-I mice by the dominant lethal test at inhalation exposure levels of 30,000, 10,000 and 3,000 ppm (6 hr/day for 5 days). The only significant mortality occurred in the groups exposed to the highest level of VC. Mice were also given ethyl methanesulfonate (200 mg/kg/day x 5, po) or cyclophosphamide (200 mg/kg on day 5, ip). Cyclo phosphamide or methanesulfonate treatment increased the number of pregnancies with early deaths. The effect was significant in weeks I and 2 for the cyclophosphamide-treated group and week 2 for the methanesuifonatc-treated group. No differences from controls were observed in the VC-treated group. As indicated by the total implants per pregnant female. VC caused no preimplantation egg losses. As demonstrated by the number of females with one or more early deaths, the number of early deaths/pregnancy, or the number of early deaths/total implants/pregnancy, VC caused no significant increase in the number of postimplantational early fetal deaths. It is concluded that, although mutagenic effects of VC have been reported, no such effects, as determined in the present study, occur in the germ cells of CD-I mice. (14 refs) 107. HUMAN, RAT AND MOUSE-LIVER MEDIATED MUTAGENICITY OF VINYL CHLORIDE IN S. TYPHIMURIUM STRAINS. Bartsch H, Malaveille C, Montesano R Int. Agency Res. Cancer, Lyons, France Int J Cancer; l5(3):429-437 1975 The mutagenicity of vinyl chloride monomer (VCM) and its presumed metabolites in Salmonella typhimurium strains as mediated by tissue fractions of mouse, rat, and human origin were studied. The male BD-IV rats (100-130 g) and maleOF-l mice (30-35 g) used were fed a Charles River CRF diet. After six hours of exposure to 20% VCM in air (vol/vol) strain TA 1530 was specifically reverted to His prototrophy. This mutagenic response was increased to 283% of the control value by mouse liver postmitochondrial fraction (9.000 x g 23 UCC 106346 supernatant); to 345% of the control by rat liver postmitochondrial fraction; and from 270-700% of the control value by four samples of this fraction from four biopsies of human livers Phenobarbitone sodium (PB) was added to the animals' drinking water (I mg/ml) for seven days before tissue fractionation in some experiments. This treatment increased the above mutagenic response to 457% for mouse liver fractions and to 383% for rat liver fractions. No cytotoxic effects of VCM were seen. Chloroacetic acid (a urinary metabolite of VCM) and chloroacetaldehyde were toxic, while chloroethanol was weakly mutagenic for TA 1530. The effects of subcellular fractions from mouse liver on the mutagenic response after six hours of exposure to 20% VCM at 37 C were: postmitochondrial fraction, 323% of control; microsomal fraction, 182%; cytosol (supernatant after 100,000 x g) 144%; and microsomal fraction plus cytosol, 522% of control. The number of His+ revertants minus the number of spontaneous mutations found when no tissue fraction was added was used as the control value. A casual relationship between VCM exposure and angiosarcoma of the liver in man has been established. 108. MUTAGENICITY and metabolism of vinyl CHLORIDE. Montesano R, Bartsch H Unit Chemical Carcinogenesis, International Agency Res. Cancer, Lyon, France Adv Tumor Prev Detect Charact; 3:242-245 1976 Studies on the mutagenicity and metabolism of vinyl chloride are reviewed. The mutagenic and/or carcinogenic effect of vinyl chloride appears to be mediated through the formation of electrophilic metabolites by microsomal mixedfunction oxidase. The pretreatment of rats with drugs that modify the activity of the enzyme results in changes in the in vitro mutagenicity of vinyl chloride. This supports the hy pothesis that the carcinogen has to be converted into electro philic and mutagenic metabolites. Pretreatment of rats with phenobarbitone increases the mutagenic response, whereas the administration of pregnenolone-16a-carbonitrile and aminoacetonitrile reduces the liver-mediated mutagenicity of vinyl chloride. Experiments involving the trapping of chloroelhyiene oxide in vitro by 4-nitrobenzylpyridine, fol lowing activation of vinyl chloride in the presence of mouse liver microsomal enzymes, an NADPH-generating system, and oxygen, support the hypothesis that monochloroethylene oxide is a primary reactive and mutagenic metabolite of vinyl chloride. 109. COMMENT ON THE MUTAGENIC EFFECTIVENESS OF VINYL CHLORIDE METABOLITES. Hussain S, Osterman-Golkar S Wallenberg Lab., Univ. Stockholm, S-104 05 Stockholm. Sweden Chem Biol Interact; l2(3-4y.265-267 1976 In the region of low doses on dose-response curves, the mutagenic effectiveness per unit dose, determined as the time integral of concentration, was found for several alkylating agents to be approximately proportional to the calculated rate of reaction at a certain low nucleophilic strength, n ap proximately equal to 2. This proportionality appears to be independent of the nature of the alkyl introduced, and for this reason the computed degree of alkylation at n equal to 2, and at a given tissue dose, may be used tentatively to estimate genetic risk. The mutagenic effectiveness of chloroethylene oxide was found to be higher than the values obtained for two standard compounds, ethylene oxide and methyl methanesulfonatc. 110. THE MUTAGENICITY OF THE CARCINOGEN VINYL CHLORIDE AND ITS COMPARISON WITH A KNOWN ALKYLATING MUTAGEN. (PP. 505-519) Loprieno N, Barale R, Baroncelli S, Bronzetti G, Cammellini A, Corsi G, Leporini C, Nieri R, Rossi AM Screening Tests In Chemical Carcinogenesis; International Agency for Res. on Cancer. (Brussels, Belgium, 9-12 June 1975, no. 12, 1976. The authors compared the mutagenic activity of methyl methanesulfonate (MMS), used as a reference chemical, with that of vinyl chloride (VCM). The mutagenic activities were determined by use of the genetic systems of two eukaryotic yeast cells (Schizosaccharomyces pombe and Saccharomyces cerevisiae), which allow the evaluation of forward mutations on the five-loci system of S pombe and mitotic gene conversions on the two-loci system of S cerevisiae or the onelocus system of S pombe. From the experimental data available, the biological effect of VCM has been attributed mainly to its conversion by microsomal enzymes to reactive metabolites of the alkylating type. For this reason, the alkylating compound MMS was chosen for comparison. In the present experiments, it was found that I mmol of MMS is equivalent to 41.7 mmol of VCM, Mitotic gene-conversion data gave a similar ratio; VCM is 10 to 40 times less effective than MMS. The values of the specific mutation rates and of the dose required for doubling the spontaneous mutation frequency indicated that VCM is converted to a highly reactive mutagenic metabolite. 111. EVALUATION of the genetic effects INDUCED BY VINYL CHLORIDE MONOMER (VCM) UNDER MAMMALIAN METABOLIC ACTIVATION: STUDIES IN VITRO AND IN VIVO. Loprieno N, Barale R, Baroncelli S, Bauer C, Bronzetti G. Cammellini A, Cercignani G, Corsi C, Gervasi G, Lep orini C, Nieri R, Rossi AM, Strcui G, Turchi G Istituto di Genetica, University, Pisa, Italy Mutat Res; 40:85-95 1976 The mutagenic activity of vinyl chloride monomer (VCM) was observed on yeast in the presence of a pure preparation of mouse (Swiss albino) liver microsomes and in the "hostmediated assay''; the gene conversion inducible by Saccharomyces cerevisiae was also observed. VCM in the presence of purified microsomcs (sedimented at 105,000 g) was converted into an active metabolitc(s) that produced gene mutations in the yeast S pombe (forward mutation) and gene conversions in two loci of diploid S cerevisiae. No mutagenic activity was found when yeast cells were treated with VCM, a phosphate buffer, or microsomes alone; this demonstrates that the enzyme necessary to metabolize VCM into a biologically reactive compound is not present in the yeast. Moreover, the compound was active in the host-mediated assay, when mice were treated with an oral dose of 700 mg/kg of VCM. These results have demonstrated that VCM is mutagenic for eukaryotic organisms and that it produces other genetic changes; this activity is liver-microsome dependent and dose dependent. It is recommended that reliable methodologies, like this mammalian metabolic activation one, for assessing the toxicological values of many industrial compounds before they reach production, be made available. 24 UCC 106347 112. VINYL CHLORIDE MUTAGENICITY VIA THE METABOLITES CHLOROOX1RANE AND CHLOROACETALDEHYDE MONOMER HYDRATE. Elmore JD, Wong JL, Laumbach AD, Streips UN Department of Chemistry, University of Louisville and Department of Microbiology, University of Louisville, Louisville, Ky. 40208 (U.S.A.) Biochim Biophys Acta; 442(3):405-4l9 1976 Mutagenicity tester strains of Bacillus and Salmonella were used to assay vinyl chloride in nutrient broth at a practical concentration level. Also screened without exogenous activation were seven potential metabolites of vinyl chloride in their pure forms as well as the related epichlorohydrin. Chlorooxirane, chloroacetaldehyde, chloroacetaldehyde monomer hydrate, chloroacetaldehyde dimer hydrate, chloroacetaldehyde trimer, and epichlorohydrin produced significant mutagenic activity in Salmonella typhimurium strains sensitive to base-pair mutation. A recombination repair deficient strain of Bacillus subtilis was inhibited in growth by these compounds, whereas excision repair deficient and wild type strains of Bacillus subtilis were relatively unaffected. On the basis of these assays a working hypothesis for the vinyl chloride carcinogenesis mechanism is proposed which involves chlorooxirane and chloroacetaldehyde monomer hydrate as the ultimate carcinogenic metabolites of vinyl chloride. (Author Abstract) 113. MUTAGENICITY OF CHLOROACETALDEHYDE, A POSSIBLE METABOLIC PRODUCT OF 1,2DICHLOROETHANE (ETHYLENE DICHLORIDE), CHLOROETHANOL (ETHYLENE CHLOROHYDRIN), VINYL CHLORIDE AND CYCLOPHOSPHAMIDE (MEETING ABSTRACT). McCann J, Simmon V, Streitwieser D, Ames BN Biochemistry Dept., Univ. California, Berkeley, Calif. 94720 Proc Natl Acad Sci USA; 72(8):3190-3193 1975 A rapid, sensitive bacterial test to detect chemical carcinogens as mutants utilizes a special set of histidine mutants of Salmonella typhimurium for reversion, and a rat (or human) microsomal system for metabolic conversion of carcinogens to their active forms. The mutagenicity of chloracetaldehyde was tested in this system because the comp ound is a possible metabolite in mammals of the large-volume industrial chemicals 1,2-dichloroethane (ethylene dichloride ; 3.5 billion kg/yr, U.S) and vinyl chloride (2.5 billion kg/yr, U.S), and of the antineoplastic agent, cyclophosphamide . Chloroacetaldehyde effectively reverted a new Salmonella bacterial tester strain (TAI00) at all levels tested (1-5 mg). Chloroacetaldehyde was hundreds of times more effective in reverting TAI00 than was chloroethanol ( ethylene chlorohydrin ). a known metabolic precursor of chloroacetaldehyde and a possible metabolite of dichloroethane and vinyl chloride; or vinyl chloride, which is itself mutagenic for TAI00. Chloroethanol was activated by rat and human liver homogenates to a more highly mutagenic form with reversion properties similar to chloroacetaldehyde. Reversion properties of cyclophosphamide after in vitro metabolic activation suggests that chloroacetaldehyde is not the active mutagenic form of this antineoplastic drug. The likely involvement of chloroacetaldehyde in the metabolism ofdichloroethanc and chloroethanol indicates that the carcinogenicity of these industrial chemicals should be thoroughly examined. 114. MUTAGENICITY AND METABOLISM OF VINYL CHLORIDE AND RELATED COMPOUNDS. Bartsch H, Malaveille C, Barbin A, Bresil H, Tomatis L, Montesano R International Agency Res. Cancer, Unit Chemical Carcinogenesis, 69008, Lyon, France Environ Health Perspect; 17:193-198 1976 Experimental data concerning the metabolism and mutagenicity of vinyl chloride (VC) and related compounds are reviewed. The data suggest that the biological effects of VC are related to its conversion by microsomal enzymes into chemically reactive alkylating agents that can bind covalently to various cellular macromolecules. The mutagenicity of VC to Salmonella typhimurium strain TAI530, which is reverted to his+ by single base-pair substitutions, was increased 28-fold after exposure to an atmosphere of 20% VC (volume/volume) in air. Hepatic microsomal mixed-function oxidases from rats, mice, and humans were equally effective in transforming VC into alkylating agents in vitro. Two of the products of reaction with the microsomal enzyme system, chloroethylene oxide and 2-chloroacetaldehyde, demonstrated potent mutagenicity in microorganisms and Chinese hamster V79 cells. (31 Refs) \ 115. INDUCTION OF GENE MUTATIONS AND GENE CONVERSIONS BY VINYL CHLORIDE METABOLITES IN YEAST. Loprieno N, Barale R, Baroncelli S, Bartsch H. Bronzetti G, Cammellini A, Corsi C, Frezza D, Nieri R, Leporini C, Rosellini D, Rossi AM Laboratorio di Mutagenesi e Differenziamento C.N.R. Via Cisanello 147/B, 56100 Pisa, Italy Cancer Res; 37(l):253-257 1977 Chloroethylene oxide and 2-chloroacetaldehyde, two metabolites of vinyl chloride, and 2-chloroethanol, a putative metabolic intermediate, were assayed for their genetic activity in the yeasts Schizosaccharomyces pombe and Saccharomyces cerevisiae. Chloroethylene oxide was found to be the most effective in inducing forward mutations in Sch pombe and gene conversions in S cerevisiae, increasing the mutation and conversion frequencies 340 and 50 times, respectively, over those of the controls. In either the presence or the absence of mouse liver microsomes, 2-chloroacetaldehyde showed only feeble genetic activity, and 2-chloroethanol was completely inactive in both yeast strains. In contrast to vinyl chloride, 2chloroacetaldehyde did not induce forward mutations in Sch pombe in the host-mediated assay in mice. The results strongly support the hypothesis that chloroethylene oxide is one of the principal mutagenic agents formed from vinyl chloride in the presence of mouse liver enzymes. (Author Abstract) 116. VINYL CHLORIDE DEPENDENT MUTAGENESIS: EFFECTS OF LIVER EXTRACTS AND A FREE RADICAL GENERATING SYSTEM (MEETING ABSTRACT). (PP. 176) Garro AJ, Guttenplan JB, Milvy P Ml. Sinai Sch Med, NY. NY 10029 Third International Symposium On Detection And Prevention Of Cancer. 1976. The relationship between vinyl chloride (VC) dependent mutagenesis and metabolic activation of VC by hepatic extracts was examined. VC itself was observed to be mutagenic for Salmonella typhimurium and mutagenesis was enhanced by the presence of mouse or rat liver extracts. The extracts prepared from mice pretreated cither with VC or the microsomal enzyme inducer, Aroclor 1254, did not produce any greater stimulation of VC dependent mutagenesis than extracts from untreated animals. These same extracts, however, differed markedly in their capacity to stimulate the 25 UCC 106348 mutagenic activity of dimethylnitrosamme, a compound which iv converted to a mutagen by an NADPH-dependent microsomal mixed-function oxidase. In contrast to what was seen with dimethylnitrosamme, the stimulatory effect of the liver extracts on VC mediated mutagenesis did not require NADPH and was still evident in liver extracts in which the microsomal mixed-function oxidase had been heat inactivated. Since VC polymerizes by a free radical reaction mechanism and since free radicals are known to be mutagenic, the possibility that a free radical generating system would stimulate VC dependent mutagenesis was examined. Free radicals were generated by photo-excitation of riboflavin and it was observed that this system did stimulate the mutagenic activity of VC. We have concluded that the mutagenic effect of VC may involve a free radical process and that the stimulatory effect of liver extracts may not be due to enzymatic activation of VC by a microsomal mixed-function oxidase. (Author Abstract) 117. STUDIES ON THE MUTAGENICITY OF VINYL C HLORIDE METABOLITES AND RELATED COMP OUNDS (MEETING ABSTRACT). (PP. 192-193) Laumbach AD, Wong JL, Streips UN Dept Microbiol and Immunol, Sch Med, Dept Chem, Univ Louisville, Louisville, KY 40201 Third International Symposium On Detection And Prevention Of Cancer. 1976. The mutagenic potential of several purified metabolites of vinyl chloride monomer (VCM) was determined by utilizing bacterial assay methods. First, a preliminary screen, the repair assay, using DNA repair deficient mutants of Bacillus subtilis was performed, then the compounds were tested quantitatively for mutagenicity with Salmonella typhimurium LT-2 strains obtained from B N Ames. From all the tested compounds the following were found to be mutagenic in the bacterial assays: chlorooxirane (chloroethyleneoxide), chloroacetaldehyde, chloroacetaldehyde hydrate, chloroacetaldehyde dimer hy drate, and chloroacetaldehyde trimer. In additional epichlorohydrin (l-chloro-2,3 epoxypropane), a related comp ound to chlorooxirane, was weakly mutagenic in our assays. All of the above compounds specifically reverted the Salmonella tester strain TA 100, indicating base pair substitution type of mutations. A recombination repair deficient strain of B subtilis, MC-1, was specifically inhibited in growth by the VCM metabolites. However, several excision repair deficient strains and the wild type (repair-positive) strain were relatively unaffected. These experiments suggest that VCM metabolites elicit recombination repair, an error prone process, for correction of damage, epichlorohydrin was not reactive in these experiments, indicating that either epichlorohydrin-induced lesions or the repair of these lesions differ from those caused by VCM metabolites. (Author Abstract) 118. THE MUTAGENICITY OF WASTE PRODUCTS FROM THE VINYL CHLORIDE INDUSTRIES (MEETING ABSTRACT). Rannug U, Ramel C Environmental Toxicology Unit, Wallenberg Lab., Stockholm. Sweden Mutat Res; 38(2): 113 1976 Vinyl chloride has been shown to possess carcinogenic and mutagenic properties. There is a risk that byproducts also possessing these properties may be formed in industrial processes involving this compound. The manufacture of vinyl chloride from acetylene and/or ethylene has given rise to a waste product, the EDC tar that contains ethylene dichloride as one of its main components. (This waste product has been dumped into the sea in large quantities.) It was tested for mutagenicity with one of the strains in the Salmonella test system (TAI535), which responded to base-pair substitutions in DNA. Ethanol, dimethyl sulfoxide, and Tween SO were used to dissolve or emulsify the tar. They produced a mutagenic effect which was approximately of the same magnitude. However, when a microsomal fraction from rat liver plus an NADPH-generating system was added, the EDC tar exhibited a considerably stronger mutagenic effect. These results suggest that there are direct as well as indirect mutagenic components in the EDC tar. 119. THE NON-MUTAGENICITY AND RECOMBINOGENICITY OF VINYL CHLORIDE IN THE ABSENCE OF METABOLIC ACTIVATION. Shahin MM Dept. Genetics, Univ. Alberta, Edmonton, Alberta T6G 2E9, Canada Mutat Res; 40(3):269-272 1976 The ability cr vinyl chloride to induce reversion and mitotic recombination in the yeast Saccharomyces cerevisiae was investigated. Strain DS was chosen for study of the induction of recombinational events and XVI8S-I4C was chosen for reversion induction in mutants. Negative results were obtained for mutagenicity and recombinogemcity of vinyl chloride. Vinyl chloride had no effect on viability, even at a concentration as high as 0.55% and treatment up to 48 hr. The results demonstrate that vinyl chloride is not mutagenic or recombinogenic in yeast under these experimental conditions. (7 refs) 120. A COMPARISON OF THE MUTAGENIC PROPERTIES OF VINYL CHLORIDE AND METHYL CHLORIDE. Andrews AW, Zawistowski ES, Valentine CR Frederick Cancer Res, Center, Frederick, MD 21701 Mutat Res; 40(3):273-276 1976 The mutagenic properties of vinyl chloride and methyl chloride were compared in Salmonella typhimurium tester strain TA 1535. A level of 23% methyl chloride was toxic to the bacteria; an inhibitory level of vinyl chloride was not reached. With the exception of 0.5% methyl chloride, all concentrations of both chemicals (0.4-15.4% vinyl chloride and 0.5-23% methyl chloride) caused a significant number of revertants. Because of the similar properties of these gases and because vinyl chloride is a mutagen/carcinogen, methyl chloride should be considered and investigated as a possible carcinogen. (9 refs) 121. MUTAGENIC EFFECTS OF VINYL CHLORIDE IN DROSOPHILA MELANOGASTER (MEETING ABSTRACT). Magnusson J, Ramel C Wallenberg Lab., Univ. Stockholm, Stockholm, Sweden Mutat Res; 38(2):1I5 1976 Genetic investigations on Salmonella have shown that vinyl chloride is converted to a mutagenic metabolite in liver microsomes. To study the effect of vinyl chloride in the gonads and the transmission of mutations to the next generation, tests with sex-linked recessive lethals in Drosophila were performed by the Muller 5 method. Males were treated with doses of vinyl chloride in the air for 3 hr and mated to Muller 5 females. A significant increase in recessive lethals was obtained both in the first and second generations; this indicated that the induction of recessive lethal mosaics took place. These results are in accordance with previous findings that Drosophila exhibits a metabolic conversion of indirect carcinogens, resembling the metabolic activation in mammals. (No refs) 26 UCC 106349 122. MUTAGENIC EFFECTS OF VINYL CHLORIDE ON DROSOPHILA MELANOGASTER WITH AND WITHOUT PRETREATMENT WITH SODIUM PHENOBARBITURATE. Magnusson J, Ramel C Environmental Toxicology Unit, Wallenberg Lab., Univ. Stockholm, Stockholm, Sweden Mutat Res: 57(3):307-3l2 1978 Exposure of wild-type Karanas 60 Drosophila males (0-2 days old) to 10,000, 100,000 or 200,000 ppm vinyl chloride (VC) gas increased the number of complete and mosaic sexlinked recessive lethals. A threshold effect was observed that may be due to a limit of the mixed-function oxidase activity. In additional experiments. Drosophila were pretreated with a 1% soln of phenobarbital sodium (PB) dissolved in water containing 1% sucrose for 24 hr, followed immediately by exposure to 1,000 or 100,000 ppm VC gas (Group I). Group 2 was treated with either concentration of VC alone, and Group 3 was not treated. PB pretreatment enhanced the number of recessive lethals over the numbers produced by Groups 2 and 3. Compared with Groups 2 (corresponding group) and 3, the total number of recessive lethals produced by PB + 10,000 ppm VC was significant, but not the number produced by PB + 100,000 ppm VC. In Groups I and 2, the higher VC dose did not produce a higher frequency of recessive lethals than the lower dose: again, there was a lack of a dose-response effect. The results indicate that the mixed-function oxidase system can be induced in Drosophila in the same way as in mammals. (12 Refs) 124. ALKYLATING AND MUTAGENIC METABOLITES OF HALOGENATED OLEFINS PRODUCED BY HUMAN AND ANIMAL TISSUES. Bartsch H, Malaveille C, Barbin A, Planche G, Montesano R International Agency Tor Research on Cancer, 69008 Lyon, France Proc Am Assoc Cancer Res: 17:17 1976 S typhimurium TA100 in the presence of a 9,000 x g sup of PB-pretreated mice was exposed to gaseous mixtures of IIX/air. Mutation rates (his**4- rev colomes/umol/ hr/plate) taken from linear regions of dose and time dependent assays either with or without (figures bracketed) NADP** + , were as follows: I: Vinyl acetate 0(0); II: 1,1-difluorethylene 0(0); III: trichloroethylene 0(0); IV: vinyl chloride 6(2): V: 1,1dichloroethylene 15(1); VI: vinyl bromide 26(9); VII: 2-chloro1.3- butadiene 51(9): VIII: l-chloro-1,3-butadiene 157(81); IX: 3.4- dichlorobutene-l 490(345). Liver fractions from 8 human biopsies converted compounds IV, VI, VII into mutagens with an activity comparable to those of untreated mouse liver. 1,4Dichloro-butene-2 was mutagenic for TAI0O and liver microsomal fractions from mouse or humans enhanced the mutagenic effect. Epoxide formation from vinyl chloride and vinyl bromide by mouse liver microsomes was demonstrated by trapping with 4-nitro (4-benzyl) pyridine. Using the same system, compound VII yielded an alkylating intermediate while compounds II, III and V did not. Thus, the conversion of these compounds to potential carcinogenic metabolites by human and animal tissues has been demonstrated. (Author Abstract) 123. THE MUTAGENICITY OF CHLOROETHYLENE OXIDE, CHLOROACETALDEHYDE, 2CHLOROETHANOL AND CHLOROACETIC ACID, CONCEIVABLE METABOLITES OF VINYL CHLORIDE. Rannug'U, Gothe R, Wachtmeister CA Wallenberg Lab., Univ. Stockholm, S-104 05 Stockholm, Sweden Chem Biol Interact: l2(3-4):25l-263 1976 The ability of four conceivable metabolites of vinyl chloride - chloroethylene oxide, chloroacetaldehyde. 2chloroethanol and chloroacetic acid * to cause base-pair substitution directly in Salmonella typhimurium TAI535 was compared. The main comparison was performed at initial concentrations from 0.1-1.5 mM. In this region, however, a mutagenic effect was observed only with chloroethylene oxide and chloroacetaldehyde, the former being approximately 20 times more effective than the aldehyde when compared on a molar basis. 2-Chloroethano! and chloroacetic acid were also studied at higher concentration (I mM-l M). and a weak mutagenic response was found with a I M 2-chloroethanoi so lution. With chloroacetic acid no enhancement of the mutation frequency could be detected. Chloroethylene oxide was found to be approximately 450 times more effective as a mutagen than chloroacetaldehyde when the comparison was based on exposure doses, defined as the time-dependent concentrations of the compounds in the treatment solutions, integrated between the times of onset and termination of treatment. Similarly, chloroethylene oxide was 10,000-15,000 times more effective as a mutagen than ethylene oxide, used as a positive control. Although chloroethylene oxide might play a part in the previously reported mutagenicity of vinyl chloride, it is more likely that it is the active metabolite of major imp ortance. as this substance has the most pronounced property of producing a high number of mutants at low doses without causing a toxic effect. 125. MUTAGENICITY AND CHROMOSOMAL ABERRATIONS AS AN ANALYTICAL TOOL FOR IN VITRO DETECTION OF MAMMALIAN ENZYMEMEDIATED FORMATION OF REACTIVE METABOLITES. Greim H, Bimboes D, Egert G, Goggelmann W, Kramer M Abteilung Toxikologie, Gesellschaft fur Strahlen-und Umweltforschung Munchen, Ingolstadter Landstrasse I, D-8042 Neuherberg, W. Germany Arch Toxicol (Berl); 39(1/2): 159-169 1977 The mutagenic activity of various chemicals was tested in bacterial and mammalian assay systems. Trichloroethylene, I,I-dichloroethylene, vinyl chloride, tetrachlorocyclopentadiene, and the nitroso derivatives of the pesticides Carbaryl, Prometryn, and Dodin were mutagenic to Escherichia coli KI2 and/or Salmonella typhimurium in the presence of metabolically active mouse liver microsomes. Under the same conditions, tetrachloroethylene, 1,2-cis- and trans-dichloroethylene, hcxachlorocyclopentadiene, carbon tetrachloride, chloroform, halothane, trichlorofluoromethane, and styrene were not activated to mutagenic species. Incubation of human lymphocytes with dimethylnitrosamine in the presence of mouse liver microsomes induced chromosomal aberrations: there was a significantly increased number of gaps, but crossovers or translocations and breaks were less frequent. It is concluded that human lymphocytes can be successfully used in metabolizing test systems in combination with mouse liver microsomes to activate potential mutagens. 126. GENETIC ACTIVITY OF ALLYL CHLORIDE. McCoy EC, Burrows L, Rosenkranz HS Dept. Microbiology, New York Medical Coll., Valhalla. NY. 10595 Mutat Res; 57(l):l 1-15 1978 The mutagenic activity of ailyl chloride (3-chloroprene) for Salmonella typhimurium TA100 and TAI535, but not 27 UCC 106350 TAI538, was demonstrated using an assay procedure that protected against evaporation of the volatile test substance. Allyl chloride also induced gene conversions in Saccharomyces cerevisiae and displayed DNA-modifying activity for Escherichia coli. These results are in contrast to a recent study that reported that allyl chloride was devoid of genetic activity for S. typhimurium, and they indicate that the volatility of the test agent is an important factor that must be taken into consideration. (30 Refs) 127. THE MUTAGENIC EFFECT OF 1,2DICHLOROETHANE ON SALMONELLA TYPHIMURIUM 1. ACTIVATION THROUGH CONJUGATION WITH GLUTATHION IN VITRO. Rannug U. Sundvall A, Ramei C Environmental Toxicology Unit, Univ. Stockholm, S-106 91 Stockholm, Sweden Chem Biol Interact; 20(1); 1-16 1978 The weak mutagenicity of 1,2-dichloroethane (1,2-DE) for Salmonella typhimurium TA1535 was increased by the ad dition of a metabolizing system, ie, the S-9 postmitochondrial rat liver fraction. This activation was further increased by the addition of reduced glutathione (GSH). No activation occurred when GSH was added in the presence of a totally denatured S-9 fraction or in the absence of this fraction. Glutathione S-transferases A and C significantly increased the mutagenicity of 1,2-DE in the presence of GSH. A synthetic conjugate, S-(2-chloroethyl)-L-cysteine, gave a strong direct mutagenic effect at 0.2-5.0 mumol/plate, concentrations at which no effects were seen with 1,2-DE. Thus, it is concluded that 1,2-DE is activated by conjugation to GSH. (24 Refs) 128. MUTAGENIC EFFECTS OF PETROL IN DROSO PHILA MELANOGASTER. I. EFFECTS OF BENZENE AND 1,2-DICHLOROETHANE. Nylander PO, Olofsson H, Rasmuson B, Svahlin H Dept. Genetics, Univ. Umea, S-901 87 Umea, Sweden Mutat Res; 57(2); 163-167 1978 The mutagenic effects of petrol, and two of its components, benzene and 1,2-dichloroethane were investigated in Drosophila melanogaster. A sex-linked genetically unstable system in D melanogaster of genotype sc z w** + was used and results were compared with the genetically stable system, zDpw**-l-69elq. Survival of flies (the total number of hatching males from 800 untreated larvae) and the frequency of mutations (the frequency of flies with red sectors) were measured. Petrol was added to the medium at I and 2-5%. Benzene (1 and 2%) and 1,2-dichloroethane (0.1 and 0.5%) were in the medium to which newly hatched larvae were transferred. A significant increase in mutation frequency occurred at both concentrations of petrol in the unstable genotype. The stable genotype showed small and insignificant increases in mutation frequency. The differences between the stable and unstable genotypes were significant at 2.5% petrol, but not at 1% petrol. Benzene, in either concentration tested, did not significantly affect the mutation frequency. High mutagenetic activity was observed in both genotypes exposed to 1,2-dichloroethane, and the unstable genotype exhibited a significantly higher mutation frequency than the stable genotype. Toxicity was also very high. It is suggested that at least part of the observed mutagenic activity of petrol is due to its content of 1,2-dichloroethane. The existence of a metabolic activating system in Drosophila is discussed. (17 Refs) 129. THE MUTAGENICITY AND METABOLISM OF l 2DICHLOROETHANE (MEETING ABSTRACT). Rannug U, Ramei C Environmental Toxicology Unit, Wallenberg Lab., Univ. Stockholm, Stockholm, Sweden Mutat Res; 53(2):25l-252 1978 The tar-like waste product from the vinyl chloride pro duction has shown mutagenic properties in the Salmonella microsome test using strain TA1535. Ethylenedichloride (1,2dichloroethane), one of the main components in the tar, was also mutagenic in the test but cannot be responsible for the mutagenicity of the total tar. In both cases an increase in the number of mutants is seen when the metabolizing system is present, but the activation of the tar is NADPH-dependent and the activation of 1,2-dichloroethane is NADPHindependent. The metabolism and mutagenicity of the latter was therefore studied in more detail with Salmonella and different metabolizing systems. From the results it can be concluded that the activation to a more potent mutagen is carried out by enzymes in the soluble fraction. This activation is further stimulated by addition of glutathione to the system, indicating a formation of a conjugate between 1,2dichloroethane and glutathione. 1,2-Dichloroethane has also been tested on the same Salmonella strain (TA-1353) with perfused rat-liver as metabolizing system. In this test the bile produced, within half an hour after the addition of 1,2dichloroethane, is strongly mutagenic, while no effect can be seen in the perfusate. A corresponding enhancement in the mutagenicity of 1,2-dichloroethane was seen if the bacteria were incubated in the presence of enzyme preparations of glutathione S-transferases. glutathione and 1,2dichloroethane. It can therefore be concluded that one metabolic pathway of 1,2-dichloroethane involves direct conjugation with glutathione. This conjugate is excreted in the bile, where the mutagenicity is found. Normally the conjugation with glutathione is regarded as a detoxication, but in this case the effect is quite the contrary. The substance is activated by the conjugation. The results also illustrate the usefulness of the mutagenicity test based on Salmonella and in vitro metabolism in tracing metabolic pathways which could be more difficult to detect in vivo, (no Refs) 130. THE MUTAGENIC EFFECT OF 1,2DICHLOROETHANE ON SALMONELLA TYPHIMURIUM. II. ACTIVATION BY THE ISOLATED PERFUSED RAT LIVER. Rannug U, Beije B Division Toxicology Genetics, Wallenburg Lab,, Univ. Stockholm, Stockholm, Sweden Chem Biol Interact; 24(3):265-285 1979 Isolated Wistar rat liver was perfused with a soln containing 1,2-dichloroethane (DCE), 1,2-dibromoethane (DBE). or 2-chloroethanol (CE), and the mutagenicities of the perfusates for Salmonella typhimurium strains TAI530 and TAI535 were tested. Bile samples (diluted 10-fold) produced by DCE-treated livers were strongly mutagenic for TA1535, the greatest values (800 and 600 mutants/plate) being observed 15 or 30 min after addition of DCE 360 micromoles (mumol) at 0 and 90 min, respectively. Bile from DBE-treated livers (I dose of 12 mumol DBE) was also mutagenic for TA1535, pro ducing 50 and 60 mutants/plate at 15 and 30 min, respectively. Bile from DCE-treated Sprague-Dawley rats was significantly less mutagenic than that of Wistar rat bile (p less than 0.001), and the former was clearly more mutagenic after 30 min than after 15 min. CE was not mutagenic in this system. The results with DCE and DBE indicated an activation through conjugation to glutathione with a subsequent excretion through the bile. Bile produced by mice treated ip with DCE 28 UCC 106351 (SO mg/kg) was also mutagenic for TAI535, the mutagenicity being greater 30 min after injection than 60 min after injection. S-(2-chloroethyl)-L-cysteine and N-acetyl-S-(2-chloroethyl)-Lcysteine were equally mutagenic for TA1535 in the concentration range 0.2-0.6 mumol/plate, whereas S-(2hydroxyethyl)-L-cysteine was not directly mutagenic. Differences and similarities in the metabolism of DCE and vinyl chloride are discussed on the basis of these results. (49 Refs) 131. TISSUE-MEDIATED MUTAGENICITY OF V1NYLIDENE CHLORIDE IN SALMONELLA TYPHIMURIUM TA1535. Jones BK, Hathway DE Central Toxicology Lab., Imperial Chemical Industries Ltd., Alderley Park, Cheshire, SK10 4TJ, England Cancer Lett; 5(1): 1-6 1978 The Ames' mutagenicity assay, modified to assess the mutagenicity of gases and vapors, was used to assess the mutagenic potential of vinylidene chloride (VDC) when incubated with a fortified mammalian kidney or liver tissue postmitochondria! supernatant (S*9 mix) from various species. Seeded dishes of Salmonella typhimurium strain TA100 or TA1535 were exposed to an atmosphere of 5% VDC in air for 72 hr. TA100 and TAI535 gave similar results. VDC was strongly mutagenic when mediated by mouse kidney (23-fold increase in mutation frequency) and liver (18-fold increase) S-9 mix from Aroclor 1254 induced animals. VDC was weakly mutagenic when mediated by S-9 mix from uninduced mouse kidney (2.3-fold increase) and liver (1.6-fold increase). VDC was weakly positive (5-fold) when mediated by liver S-9 mix from similarly induced rats, but it was not mutagenic (less than or equal to 1.2-fold) when mediated by kidney or liver S-9 mix from noninduced rats. VDC showed weak mutagenicity (3fold) when tested in a system mediated by liver S-9 mix from a human who had received long-term phcnobarbital medication, but it was not mutagenic when mediated liver S-9 mix from noninduced marmosets or a noninduced human. Thus, the mutagenic potential of VDS depends considerably on the degree of activation of relevant drug-metabolizing enzymes. The results agree with the greater availability in treated mice than in rats of the reactive VDC metabolites 1,1dichloroethylene oxide and chloroacetyl chloride and with the VDC carcinogenicity found in mice but not in rats. The data suggest that the limited number of primates examined respond more like rats than mice with regard to generation of alkylating metabolites and their reaction with bacterial DNA. (10 Refs) 132. MUTAGENICITY OF VOLATILE ANESTHETICS (MEETING ABSTRACT). Baden J, Wharton R, Hitt B, Brinkenhoff M, Simmon V, Mazze R Stanford University and Va Hospital, Palo Alto, Ca 94304 and Stanford Research Institute, Menlo Parle, Ca 94025 Fed Proc: 35(3):4IO 1976 Surveys of operating room personnel suggest that inhalational anesthetic agents may be carcinogenic. Therefore, an in vitro microbial system was used to test the mutagenicity of these compounds. Halothane was tested first because it is the most commonly used volatile anesthetic and because its metabolism proceeds through highly reactive intermediates. Two histidines requiring mutants of S typhimurium (developed by B Ames) TA 98 and TA 100, were used. Halothane in concentrations ranging from 0.1% to 30%, was incubated with bacteria in the presence or absence of NADP, glucose-6-phosphate, and hepatic post mitochondrial supernatant prepared from rats treated with Aroclor 1254. After incubation for 1 hour at 37 degrees C, the reaction mixtures were overlayed onto glucose minimal media. The plates were incubated for 48 hours at 37 degrees C and revertant colonies counted. Vinylidene chloride and 4aminobiphenyl were positive controls for TA 100 and TA 98, respectively, Trifluoroacetic acid, the major metabolite of halothane, and urine from patients anesthetized with halothane were also tested in this system. Halothane , trifluoroacetic acid, and patients' urine were not mutagenic. However, preliminary studies suggest that trichloroethylene, an industrial solvent and anesthetic agent, is mutagenic. (Author Abstract) 133. ACTIVATION MECHANISMS IN CHLORINATED ALIPHATIC COMPOUNDS. EXPERIMENTAL POSSIBILITIES AND CLINICAL SIGNIFICANCE. Henschler D Toxikologisches Institut der Universitat, Koellikerstr. 2, 8700 Wurzburg, W. Germany Arzneim Forsch; 27(9b): 1827-1832 1977 Asymmetric chlorinated ethylene (tri-1,1dichloroethylene and vinyl chloride) are mutagenic in a modified Ames test system whereas symmetrically substituted molecules are inactive. These differences are attributed to the electron withdrawl effect of chlorine in the asymmetric molecules. The in vivo and in vitro rearrangement mechanisms in the biotransformation of these compounds are outlined. (20 Refs) 134. MUTAGENICITY OF INDUSTRIAL COMPOUNDS EVALUATED BY MEANS OF YEAST GENETIC SYSTEMS (MEETING ABSTRACT). Fumero S, Mondino A Istituto di Ricerche Biomediche 'A Marxer', Ivrea, Italy Mutat Res; 53(2): 189 1978 There are many chemical compounds of wide use in the chemical industry (solvents, halogenated hydrocarbons, aromatic polycyclic hydrocarbons, monomers employed in the resin and plastic industry) which have been shown to produce different genetic effects (reverse and forward mutations, geneconversions and mitotic recombinations) on yeasts (Saccaromyces cerivisiae and S pombe). The use of yeasts, moreover, in the mutagenic analyses of such a class of comp ounds has resulted in the study of the 'in vitro' and 'in vivo' metabolic conversion of the original compounds tested. We will present data related to the mutagenic analyses of some other chemicals (trichloroethylene and its epoxidic metabolite, epichlorohydrine) in comparison with known chemical carcinogen: 'in vivo' studies with host-mediated assay have provided data of extreme interest in the evaluation of the mutagenic activity of these compounds for a prediction of their possible cancerogenic activity, (no Refs) 135. MUTAGENIC ACTIVITY OF CHEMICALS IDENTIFIED IN DRINKING WATER (MEETING ABSTRACT). Simmon VF, Kauhanen K, Mortelmans K, Tardiff R Stanford Res. Inst., Menlo Park, CA Mutat Res: 53(2):262 1978 Approx 300 chemicals have been identified in finished drinking water in the US. Some of these chemicals are known to be carcinogens in rodents (eg, aldrin, carbon tetrachloride) and/or humans (benzopyrene). We have begun to analyze these chemicals for their mutagenic activity in micro organisms. Of 128 chemicals that we have tested, 21 were mutagenic in preliminary spot tests on Salmonella typhimurium TAI00 (without metabolic activation). We have obtained dose-dependent mutagenic responses with 19 of these compounds (benzopyrene, bromochloromethane. 29 UCC 106352 bromodichloromethane, bromoform, bromomethane, n-butyl bromide, sec-butyl bromide, tert-butyl bromide, technical grade chlordane, chlorodibromomethane, bis(2-chloroethyl) ether, dibromomethane, 1,2-dichloroethane, 1,1* dichloroethylene, hexachloro-1,3-butadiene, iodomethane, methyl chloride, methylene chloride and vinyl chloride) in strains of S typhimurium. Additional mutagenicity assays using Escherichia coli WP2 and Saccharomyces cerevisiae D3 are underway, (no Refs) diffusion, and the size of the zone of growth inhibition. None of the substances tested induced mutations in S typhimurium TA 1538. Since many of the compounds tested are widely used in industry and in the home, further determination of their potential hazard to health is indicated. 136. THE MUTAGENIC ACTIVITY OF HALOGENATED COMPOUNDS FOUND IN CHLORINATED DRINKING WATER. Simmon VF, Tardiff RG Microbial Genetics Program, Stanford Res, Inst. International, Menlo Park, CA, 94025 Water Chlorination; 2:417-431 1978 The mutagenic activities of 22 halogenated compounds found in chlorinated drinking water were determined using the Ames Salmonella/microsome procedure with S. typhimurium strains TA1535 and TA100. Two known carcinogens, carbon tetrachloride and chloroform, were not mutagenic in this system, probably because mutagenic metabolites were formed in insufficient amounts or they were so unstable that they did not survive long enough to penetrate the bacteria and interact with the DNA. Known carcinogens that were mutagenic were vinyl chloride, vinylidine chloride, 1,2-dichloroethane, 1,1,2trichloroethylene, bis-(2-chloroethyl)ether, methyl iodide, and bromoform. Mutagenic activity was also observed with bromodichloromethane, chlorodibromomethane, methylene bromide, bromochloromethane, methylene chloride, methyl bromide, methyl chloride, 2-chloropropane, l-chloropropene, bis(2-chloroisopropyl)ether, n-butyl bromide, t-butyl bromide, and s-butyl bromide. The results indicate that the number and amount of alkyl halides in potable water should be reduced to lessen possible health hazards. (20 Refs) 137. THE MUTAGENICITY AND DNA-MODIFYING EFFECT OF HALOALKANES. Brem H, Stein AB, Rosenkranz HS Coll. Phys. Surg., Columbia U., New York, N. Y. Cancer Res; 34:2576-2579 1974 A series of haloalkanes were tested for their ability to inhibit the growth of normal (pol A+)and DNA polymerase l-deficient (pol AI-) Escherichia coli. These compounds were also tested for the mutagenic effects on Salmonella typhimurium. All of the haloalkanes examined preferentially inhibited the growth of the pol AI- strain of E coli. The bromoalkanes appeared to be more active than their chloro analogs (e g, 1,2-dibromoethane (1,2-DBE) was more active than 1,2-dichloroethane (1,2-DCE) and tetrabromocthane was more active than tetrachloroethanc). The mixed haloethane 1bromo-2-chloroethane had an activity intermediate to those of 1,2-DBE and 1,2-DCE. When the bromine was on the same carbon, the biological activity was enhanced (1,1-DBE was more active than 1,2-DBE), although when the halogens were on different carbon atoms, the distance between them had no effect on the activity. All of the haloalkanes tested, with the exception of 1,1,2,2-tetrabromoethane, were mutagenic for S typhimurium TA l530andTA 1535. The number of mutations was related to the amount of reagent added, the rate of 138. TISSUE MEDIATED MUTAGENICITY AND CARCINOGENESIS (MEETING ABSTRACT). (PP. 73) Bartsch H International Agency for Research on Cancer, Unit of Chemical Carcinogenesis, 150 cours Albert Thomas, 69008 Lyon, France. Third International Symposium On Detection And Prevention Of Cancer. 1976. Studies on the mechanism of the organotropic action of chemical carcinogens provide better criteria for an extrapolation from animal data to tumorigenic processes in man if human tissues or fluids are included in the experimentation. For many chemical carcinogens, the generation of specific ultimate reactive metabolites by one- or multi-step activation processes and their subsequent covalent binding to information controlling cellular macromolecules is thought to induce mutagenesis and carcinogenesis. Mutagenicity assays permit a quantitative comparison of the enzymic capacity for carcinogen activation in animal and human target and non-target tissues. Tissue-mediated mutagenicity of vinyl chloride and certain N-nitroso comp ounds was measured using S typhimurium strains in agar-plate assays containing 9,000 x g liver or lung fractions from untreated rats or from humans, either by exposing the Petri dishes to gaseous mixtures of the test compound with air (vinyl chloride, 20% by volume for 6 hours) or by incorporation of the substrate into the soft agar (0.5-10 microMol of Nnitrosamines per plate). The relative capacity of tissues from human individuals (represented by A, B, C, D, X, Y and Z) to convert various substrates into mutagens was as follows (rat = I0O): vinyl chloride; A, B. C, D(275, 101, 93, 80); Nnitrosomorpholine: A, B, C, D (90. 50, 40, 30); Nnitrosopyrrolidine: Z, X, Y (115, 90, 50); N-nitrosopiperidine: Z, Y, X (215, 185, 85); N-nitroso-N'-methylpiperazine: Z, Y, X (3,200, 1800, 400). Liver fractions from untreated rats and human specimens Z and Y were unable to convert Nnitrosodi-n-propylamine and N-nitroso-di-n-butyl-amine into mutagens. With the latter two compounds a tissue-mediated mutagenicity was detected with liver fractions from phenobarbitone pretreated rats. With the hepato-carcinogen N-nitrosomorpholinc as substrate, no mutagenic action was delected after incubation with rat and human lung fractions. The data indicate that human liver specimens can convert some N-nitroso compounds and vinyl chloride into electro philic mutagenic metabolites as efficiently as rat tissues. Enz ymic capacity of different human specimens varied 2- to 8fold. Analysis of a larger number of human individuals by this technique may eventually allow a correlation between genetic background or induced state of carcinogen activating enzymes and the individual susceptibility towards certain carcinogens. Organ specific activation of chemical carcinogens appears to be a prerequisite but not a finally determining factor for the induction of tumors in experimental animals and probably man: examples will be presented from the in vitro studies which support the concept that, for certain carcinogens, the formation of short-lived ultimate metabolites in situ can be correlated with the site of tumor formation. (Author Abstract) 30 UCC 106353 139. THE PREDICTIVE VALUE OF TISSUE-MEDIATED MUTAGENICITY ASSAYS TO ASSESS THE CARCINOGENIC RISK OF CHEMICALS. (PP. 467-491) Bartsch H, Malavcille C, Montesano R Screening Tests in Chemical Carcinogenesis Proceedings of a workshop organized by IARC and the Commission of the European Communities and held in Brussels, Belgium, 9-12 June 1975. Montesano R, Bartsch H, Tomatis L, Davis W, ed.. International Agency for Research on Cancer. (Lyon, France); IARC Scientific Publications No. 12, 1975. The Salmonella microsome system was used to assess the in vitro Salmonella mutagenicity assay for predicting possible carcinogenic effects of chemicals, to compare the capacity of different tissues and different species to convert chemical substances into mutagens, and, in particular, to investigate the metabolic activation of nitrosamines and chlorinated olefinic hydrocarbons. The mutagenicity of a series of cyclic nitrosamines in S typhimunum strain TA 1530 was similar in liquid suspension and in soft agar, but discrepancies arose when a series of dialkylnitrosamines of the form (CnH2 n + "n*2# N-NO with different chain lengths were considered. The dialkylnitrosamines with n 3 did not show an enzymemediated response in the liquid incubation system, and in the soft agar metabolically activated dimethylnitrosamine and diethylnitrosamine exhibited negligible mutagenic activity. These Endings reflect false negatives from both systems, perhaps brought about by the deterioration of microsomal enzymes by lipid peroxidation in the liquid system and by the trapping of short-lived alkylating molecular species by nucleo philic components of the agar. When the mutagenicity of a series of propyluted nitrosamine derivatives was studied with microsomes from hamster and rat tissues, there was not a good correlation between tissue-specific activation to mutagenic intermediates and the known susceptibility of different tissues to carcinogenesis. A better correlation was seen when the activation to mutagenic metabolites of vinyl chloride by microsomes derived from rat and mouse liver, kidney, and lung tissues was studied. The major enzymic activities were located in both liver and kidney, sites where angiosarcoma and nephroblastoma are induced in animals exposed to vinyl chloride. No tissues studied exhibited a higher enzymic capacity than the liver to bring about the production of mutagenic metabolities. Microsomes from human livers were capable of generating mutagenic metabolites from Nnitrosomorpholine and vinyl chloride. Some aspects of the metabolism of chlorinated olefinic hydrocarbons are discussed further. (56 refs) 140. METABOLIC ACTIVATION OF CHLORINATED ETHYLENES: DEPENDENCE OF MUTAGENIC EFFECT ON ELECTROPHILIC REACTIVITY OF THE METABOLICALLY FORMED EPOXIDES. Henschler D, Bonse G Institut fur Toxikologie, Univcrsitat Wurzburg, Versbacher Landstrasse 9, D-8700 Wurzburg, W. Germany Arch Toxicol (Berl); 39(l/2):7-l2 1977 Investigations of the chemical reactivity, biotransformation, and toxicity of the chloroethylenes are reviewed. In chlorinated ethylenes, the chlorine susbstitution exerts, by its electron-withdrawal effect, a stabilization of the molecule that increases with the number of chlorine residues. Epoxides are short-lived metabolic intermediates that rearrange to give two possible products; acylchlorides (as with tetra-, tri-. and 1,1-dichloroethylenes) or aldehydes (1,2-ctsand 1,2-trans-dichloroethylenes and vinyl chloride). The aldehydes subsequently undergo reduction and oxidation to alcohols and acids, respectively. The chlorinated ethylenes were tested for mutagenic potential in a modified Ames system, and three members of the group were active: vinyl chloride, vinylidene chloride, and trichloroethylene. The molecular feature common to the active molecules is an asym metric chlorine substitution, but in the inactive compounds there is a symmetric distribution of the chlorine residues. It is hypothesized that the increased electrophilicity caused by the asymmetrical chlorine substitution offers an enhanced chance for alkylating reactions of the epoxides, which overpower the deactivation mechanisms of conjugation, rearrangement, and hydrolysis. The three mutagenic chloroethylenes have also produced carcinogenic effects in animals. 141. MUTAGENICITY OF HALOGENATED ALKANES AND THEIR DERIVATIVES. Rosenkranz HS Dept. Microbiology, New York Medical Coll., Valhalla, NY, 10595 Environ Health Perspect; 21:79-84 1977 The DNA-modifying activity and mutagenicity of some haloalkanes, haloethanols, and 2-haloacetaldehydes were investigated. DNA-modifying activity was determined by comparing ratios of the areas of zones of inhibition around platings of DNA-polymerase-deficient (polA-) and non deficient (polA + ) Escherichia coli strains. Mutagenicity was assayed by counting revertants of Salmonella typhimurium strains from histidine-dependent to histidine-independent growth. Most active haloalkanes in the E coli assay were tetrabromoethane and 1,1-dibromoethane; least active was 1,2-dichloroethane. All the haloalkanes were mutagenic (base substitutions) for S typhimurium strains TA 1530 and TA 1535, but none for TA 1538. The greatest mutagenic activity was achieved with 1,2-dibromoethane and 1.5dibromopentane. The order of reactivities of the haloalkanes differed in the two tests. Of the haloethanols tested, 2iodoethanoi had the most DNA-modifying activity, 2bromoethanol was the most mutagenic, and ethanol and 2fluoroethanol were without either activity. 2Chloroacetaldehyde was somewhat mutagenic for strain TA 1535, but 2-bromacetaldehydc was not mutagenic at all. Both compounds had some DNA-modifying activity. The chemical basis of the mutagenic action of these compounds is discussed. (37 Refs) 142. GENETIC AND TOXICOLOGICAL EFFECTS OF ALPHA-BENZENE HEXACHLORIDE, DIBUTYL PHTHALATE AND TRICHLOROETHYLENE ON SACCHAROMYCES CEREVISIAE STRAIN XV185-14C FOR REVERSION STUDIES (MEETING ABSTRACT). Shahin MM, Von Borstel RC Dept. Genetics, Univ. Alberta, Edmonton, Alberta, Canada T6G 2E9 Mutat Res; 53(l):84-85 1978 Saccharomyces cerevisiae strain XV185-I4C for reversion studies was used to investigate the genetic activity of alphabenzene hexachloride, dibutyl phthalate and trichloroethylene. The data indicate that none of the three compounds tested was genetically active when yeast cells were tested in phosphate buffer (pH 7.0) in the absence of a metabolic conversion system. This was observed at different concentrations of the compounds used, and at varous survival levels. On the other hand, AF-2 (furylfuramide) and EMS (ethyl methanesulfonate) which were used as positive, controls exhibited mutagenic activity as expected. The results of the experiments with XV185-I4C and mice liver microsomal fraction and all needed components for metabolic conversion did not alter the non-mutagenic response of alpha-benzene hexachloride or. dibutyl phthalate. However, there is a mutagenic effect after treatment with trichloroethylene. Trichloroethylene appears to induce frameshift as well as base 31 UCC 106354 substitution mutations. Trichloroethylene is far more lethal to yeast cells than alpha-benzene hexachloride or dibutyl phthalate. 143. CHARACTERIZATION OF S-9 ACTIVATION OF DBC'P IN THE SALMONELLA TEST SYSTEM (MEETING ABSTRACT). Biles R, Connor T, Trieff N, Legator M Univ. Texas Medical Branch, Galveston, TX, 77550 Pharmacologist; 20(3); 155 1978 1,2-Dibromo-3-chloropropane (DBCP) has little direct mutagenicity in the Salmonella test system. However, pure DBCP is quite mutagenic when activated to the ultimate mutagen using S-9 preparations from Aroclor pre-treated rats. We utilized such S-9 and S typhimurium TA1535 to characterize the responsible mutagenic activation components. S-9 was purified by centrifugation (10,000 and 105,000 x gravity) to obtain the pure microsomal preparation. The concentrations of DBCP tested ranged 10-500 ug/plate and produced dose-response mutagenicity. Standard S-9 preparations were found to be heat labile and require NADPH generating systems for mutagenicity. With fractionated S-9, the microsomal fraction alone was responsible for mutagenic activity while the supernatant did not activate DBCP. The microsomes produced greater mutagenic activity at lower doses than the parent S-9. When microsomes and supernatant were recombined, mutagenicity was identical to the parent S-9. The mutagenic activity was reduced as glutathione was added in increasing amounts to both the parent S-9 and pure microsomes. Thus, glutathione inactivates rather than activates DBCP, which is contrary to recent findings for activation of ethylene dibromide and 1,2-dichloroethane. (no Refs) 144. THE INFLUENCE OF CONTAMINANTS ON THE MUTAGENIC ACTIVITY OF DIBROMOCHLOROPROPANE (DBCP). Biles RW, Connor TH, Trieff NM, Legator MS Div. Environmental Toxicology, Dept, Preventive Medicine and Community Health, Univ. Texas Medical Branch, Galveston, TX, 77550 J Environ Pathol Toxicol; 2(2):3QI-3I2 1978 The causative agent(s) and mechanism of mutagenic activity of technical grade (TG) 1,2-dibromo-3-chloropropane (DBCP) were investigated in the Ames mutagenesis test using Salmonella typhimurium TAI535. Without metabolite activation, TG DBCP was weakly mutagenic in the concentration range 50-1,000 ug/plate, whereas pure DBPC showed little or no mutagenicity in the same concentration range. Purification of the TG compound by distillation resulted in a first distillate with more than two-fold greater mutagenicity than the TG compound and a second distillate with mutagenicity similar to that of the TG compound. The first distillate was found to contain epichlorohydrin (ECH), which was present in the TG compound in an amount close to 1.5% by wt, as well as an unidentified compound. The second distillate contained smaller amounts of both compounds. The amount of ECH in the TG sample was calculated for each dose level tested, and the number of revertanis obtained in tests of this sample could be attributed solely to the calculated amount of ECH in each test dose. Both TG and pure DBCP were strongly mutagenic in the concentration range 20-200 ug/plate after S-9 metabolic activation. Results similar to those of the Ames test were obtained using a desiccator technique. The data indicate that in the direct in vitro evaluation, the mutagenicity of DBCP can be attributed almost entirely to the addition of the stabilizer ECH. (22 Refs) 145. MUTAGENICITY OF PESTICIDES CONTAINING 1,3-DICH LORO PROPEN E. De Lorenzo F, DeglTnnocenti S, Ruocco A, Silengo L, Cortese R I and 11 Cattedra di Chimica Biologica, Univ. Naples, Via Sergio Pansini 5, 80131 Naples, Italy Cancer Res; 37(6); 1915-1917 1977 The widely used pesticides Telone and D.D. soil fumigant were tested for mutagenicity in Salmonella typhimurium strains TAI978, TAI535, TAI00, TAI537, and TA98. D.D. soil fumigant is composed of 40% 1,3-dichloropropene, 40% 1.2- dichloropropane, and 20% other unknown chemicals. Telone is composed of 30% cis-1,3-dichloropropene, 30% trans-1,3-dichloropropene, 20% 1,2-dichloropropane, 5% 2.3dichloro-l-propene, 2% ally! chloride, and about 15% unknown chemicals. Both pesticides were mutagenic in strains TAIOO and TAI535 in the presence and absence of a rat liver activating system; both pesticides were mutagenic in strain TA1978 only in the presence of such a system. The cis and trans isomers of 1,3-dichloropropene were strongly mutagenic in strains TA1535 and TA100, which are sensitive to base-pair substitutions, and only weakly mutagenic in strain TAI978. 2.3- Dichloro-l-propene showed similar behavior. 1,2Dichloropropane was mutagenic in strains TA1535 and TA100 but only at concentrations 500 times higher than those of dichloropropene. The mutagenic properties of the purified components do not fully account for the mutagenicity of the commercial preparations; the difference might be due to the presence of unknown components. (12 Refs) D. Teratogenicity of Vinyl Chloride and Related Compounds in Experimental Animals The reader may also find the following abstract of interest: 79 146. STUDY OF THE TERATOGENIC AND EMBRYOTOXIC EFFECT OF VINYL CHLORIDE IN CFY RATS. Ungvary GY, Hudak A, Tatrai E. Lorincz M, Folly G Orszagos Munka- es Uzemegeszsegtan Intezet, Budapest, Hungary Egeszsegtudomany; 21(4):363-369 1977 Inhalation of vinyl chloride (4,000 mg/m1) between the 8th and 14th days of pregnancy increased the absolute and relative wt of the liver of pregnant CFY rats significantly, but there was no significant increase in resorption, embryonal mortality, and developmental anomalies. In addition, vinyl chloride was found in the maternal and fetal blood and amniotic fluid following exposure of the mothers to 5,50033,000 mg/m1 vinyl chloride for 2.5 hr on the 18th day of pregnancy. (31 Refs) 147. A TERATOLOGIC EVALUATION OF PLASMASOLUBLE EXTRACTS OF POLYVINYL CHLORIDE PLASTICS IN RATS (MEETING ABSTRACT). Chen TS, Fernandes J, Lewandowski M Travenol Laboratories, Inc., Morton Grove, 1L, 60053 Fed Proc; 38(3,partl);438 1979 Plasma extracts of two polyvinyl chloride plastics (designated PL-130 and PL-146) containing diethylhexyl phthalate (DEHP) as a plasticizer and used in the manufacture of parenteral fluid storage bags were administered iv to pregnant rats daily from the 6th through 15th day of gestation. Two groups of rats received PL-130 extracts in doses equivalent to 1.3 and 4.7 mg DEHP/kg per day. and two 32 UCC 106355 groups received PL-146 extracts in doses equivalent to 1.4 and 5.3 mg DEHP/kg per day. The higher doses are similar to that predicted for a 60-kg man receiving an exchange transfusion of 21-day-old blood. A control group of rats received the plasma vehicle only. No differences were seen in growth rates or behavior between control and treated groups. There were no significant effects of fetal wt, crown-rump, and transumbilical distances. The numbers of live and resorbed fetuses were not significantly different in control and treated groups. The incidence of gross external, skeletal, and visceral defects was similar in all groups and was not statistically different between control and treated groups. It is concluded that plasma extracts of PL-130 and PL-146 were not teratogenic when administered iv to pregnant rats during the critical period of organogenesis, (no Refs) 148. EFFECTS OF VINYL CHLORIDE EXPOSURE ALONE AND IN COMBINATION WITH TRYPAN BLUE - APPLIED SYSTEMATICALLY DURING ALL THIRDS OF PREGNANCY - ON THE FETUSES OF CFY RATS. Ungvary G, Hudak A, Tatrai E, Lorincz M, Folly G Dept. Experimental Pathology, State Inst. Occupational Health Budapest, H-1450 Budapest P.O.B. 22, Hungary Toxicology; 11(1 ):45-54 1978 The teratogenic and embryotoxic effects of vinyl chloride and/or trypan blue were investigated in CFY rats. VC was found in the fetal and maternal blood as well as in the amniotic fluid of pregnant CFY rats exposed to an atmospheric concentration of 5,500, 18,000, or 33,000 mg/m' VC for 2.5 hr on gestation day 18, indicating the permeability of the placenta to the agent. Investigation of the offspring of pregnant rats exposed continuously to 4,000 mg/mJ VC during the first, second, or last trimester sho-cd that VC has no teratological or embryotoxic effects when applied during the second or last trimester. However, exposure to VC during the first trimester resulted in an increased fetal mortality and in the manifestation of embryotoxic effects. Fetal losses and the induction of CNS malformations due to trypan blue (50 mg/kg sc on gestation days 7 and 8) were not potentiated by 4,000 mg/m! VC. Occupational exposure of women of childbearing age may be hazardous. (34 Refs) 149. INHALATION STUDIES TO EVALUATE THE TERATOGENIC AND EMBRYOTOXIC POTENTIAL OF BETA-CHLOROPRENE (2-CHLOROBUTADIENE-U). Culik R, Kelly DP, Clary JJ Haskell Lab. Toxicology and Industrial Medicine, E.I. du Pont de Nemours and Co., Inc., Wilmington, DE, 19898 Toxicol Appl Pharmacol; 44(l):8l-88 1978 Inhalation studies were performed to evaluate the teratogenic and embryotoxic potential of beta-chloroprene (2chlorobutadiene-1,3) (BC). Soviet Union researchers recently reported that BC, used in large quantities to manufacture neo prene rubber, was embryotoxic, teratogenic, and mutagenic at atmospheric concentrations below the existing Soviet maximum allowable concentration of I ppm. Two studies were carried out on pregnant rats. Both experimental groups were exposed by inhalation to 0, I, 10, and 25 ppm of betachloroprene for 4 hr daily. Fifty rats per group (in the first study) were exposed on days I through 12 and sacrificed on day 17. The purpose of this study was to evaluate the em bryotoxic potential of BC. In the second teratology study, 25 rats per group were exposed on days 3 through 20 and sacrificed on day 21 of gestation. In a reproduction study, male rats were exposed to 25 ppm of BC (4 hr daily for 22 days) and bred with untreated virgin females. In the female groups, no maternal, embryonal, or fetal toxicity was observed and the reproductive capability of males was not impaired. These results indicate that 25 ppm of BC.the present threshold limit value, is not embryotoxic or teratogenic and does not impair reproductive capability of male rats. These results are at variance with previous findings of studies conducted in the Soviet Union. (16 Refs) 150. INHALATION OF ETHYLENE DIBROMIDE DURING GESTATION BY RATS AND MICE. Short RD, Minor JL, Winston JM, Seifter J, Lee CC Dept. Pharmacology and Toxicology, Midwest Res. Inst., Kansas City, MO, 64110 Toxicol Appl Pharmacol; 46(1); 173-18 I 1978 The teratogenic potential of ethylene dibromide (EDB) was evaluated in rats and mice. Charles River CD rats and CD-I mice were housed in chambers containing 20, 38, or 80 ppm EDB for 10 days, beginning on day 6 of gestation. Animals were sacrificed on day 18 or 20 of gestation, and fetuses were examined for anomalies. Rats exposed to 38 and 80 ppm EDB suffered wt loss, but deaths, reduced number of implants, and evidence of embryotoxicity were seen only in those exposed to 80 ppm. The incidence of fetal soft-tissue anomalies found in treated rats did not differ significantly from that of controls; however, the percentage of fetuses with normally ossified centra was significantly lower in the group exposed to 20 ppm EDB than in controls. Deaths occurred in mice exposed to 38 and 80 ppm, and in those exposed to 20 ppm, there was an increase in the percentage of late resorptions and a decrease in fetal body wt. Exencephaly was seen in fetuses of mice exposed to 20 ppm, and the incidence of some skeletal anomalies was significantly greater in fetuses of EDB-exposed mice. These fetal morphological changes, however, occurred at concentrations that also affected maternal welfare. Consequently, EDB was judged to have little primary effect on development. (10 Refs) E.Transforming Activity of Vinyl Chloride and Related Compounds in Animal Cell Cultures 151. VINYL CHLORIDE EXPOSURE INDUCED LYM PHOCYTE TRANSFORMATION IN SPLENIC CULTURES (MEETING ABSTRACT). Sharma RP, Gchring PJ Utah State Univ., Logan, UT, 84322 Fed Proc: 37(3):502 1978 Vinyl chloride (VC) disease has been suggested as an im mune complex disorder. The influence of VC exposure on selected immunologic parameters of mice and rabbits was studied. In male mice, inhalation of VC (10,000 and 1,000 ppm for 6 hr/day, 5 days/wk) for up to 8 wk caused no toxic effects, as indicated by body wt gain, clinical hematology, or organ wt, with the exception of an increase in spleen wt at the highest exposure level. After 2 wk of exposure to 1,000 ppm and after 4 and 8 wk to all levels, cultured splenic lymphocytes showed an increase in DNA synthesis (as measured by H3-thymidine uptake by cells in culture). The response of splenic lym phocytes to phytomitogens (phytohemagglutinin and pokewecd mitogen) at the above levels of VC exposure was increased severalfold. In rabbits, exposure to 1,000 ppm VC caused a slight but inconsistent rise in serum immunoglobulin levels. Rabbits immunized with tetanus toxoid and Freund's adjuvant revealed no VC-related changes in scrum antitetanus titers, skin reactivity to tuberculin, or the number of plasma cells in popliteal lymph nodes. There was no increase in im munization induced lymphocyte transformation in either mice or rabbits. The results indicated an increase of lymphocyte 33 UCC 106356 transformation in splenic cultures that may be related to the immune complexes observed m VC syndrome, 152. MALIGNANT TRANSFORMATION OF A BABY HAMSTER LUNG CELL LINE BY 2CHLOROBUTADIENE (MEETING ABSTRACT). (PP. 78) Papadopoulo D, Markovits P, Beesau O, Hubert-Habart M Fondation Curie-lnstitut du Radium. 26 rue d'Ulm, 75005Paris, France Fourth Meeting of the European Association for Cancer Research Held at Universite de Lyon, September 13-15, 1977. European Association for Cancer Research, Lyon, France 1977. From trypsin-dispersed baby hamster lung tissue, a control hamster lung cell line was established, which remained very stable and non-transplantable during a long period of time in vitro. Semiconfluent cell cultures of this line were exposed to either 1, 10, or 100 microg/ml for 6 consecutive wk, or, to either 500 or 1000 microg/ml for two days, of 2chlorobutadiene (CB). Three and one-half mo after the start of the treatment, morphologically altered spindle-shaped celts appeared in all the treated cultures. While these cells were dominant in the CB 1 microg/ml treated cultures, only infrequent groups of them were observed in the cultures treated previously with higher concentrations of CB. Cultures treated with I microg/ml of CB caused tumors in all hamsters grafted 3.5 mo after starting treatment. Cells treated with higher concentrations of CB provoked tumors only 8 mo after the starting of treatment. Histologically, the tumors were fibrosarcomas and the ones caused by cells treated with 1 and 10 microg/ml were highly malignant. It seems that 1 microg/ml of CB is an optimal concentration for malignant transformation. This work is the first experimental demonstration of the malignant transforming capacity of 2chlorobutadiene. which was shown previously to be mutagenic. Experiments are underway to confirm these preliminary results, (no Refs) 153. IN VITRO MALIGNANT TRANSFORMATION OF CELLS OF WHOLE EMBRYOS. FETAL BRAIN, AND NEWBORN LUNG OF HAMSTER (MEETING ABSTRACT). Levy S. Markovits P, Beesau O, Benda P Fondation Curie-lnstitut du Radium, Section de Biologie, 26 rue d'Ulm, 75 231 Paris cedex 05, France J Microsc (Paris); 27(1); 17a 1976 Chemical carcinogens were used to transform cultured cells from the whole embryo, fetal brain, and newborn lung of hamsters. Cells from the whole embryo were exposed to polycyclic hydrocarbons and heterocyclic mitogen-containing compounds for 24-48 hr. After 2 mo growth, the ceils lost contact inhibition and their growth rate increased. The ability to produce sarcomas after an sc graft in syngeneic hamsters did not appear until 5 mo after treatment. Lung cells exposed to 7,l2-benzo(a)anthracene under the same culture conditions underwent malignant transformation more slowly. Intraocular grafts proved more likely to produce tumors than sc grafts. When lung cells were exposed to repeated doses of chlorobutadiene (3 doses every 14 days) over a 16-mo culture period, transformation took place in 3.5 mo. Fetal brain cells exposed to nitrosomethylurea underwent transformation in 4 mo. Glial cell tumors occurred after intraocular or intracerebral grafts of the transformed cells. It is believed that malignant transformation of lung and nerve cells by chemical carcinogens occurred for the first time in these experiments. (0 Refs) 154. TRANSFORMING ACTIVITIES OF TRICHLOROETHYLENE AND PROPOSED INDUSTRIAL ALTERNATIVES. Price PJ. Hassett CM. Mansfield Jl Microbiological Associates. Torrey Pines Res. Center, 2945 Science Park Road, La Jolla, CA, 92037 In Vitro; 14(3):290-293 1978 The carcinogenicity of trichloroethylene (TCE) and three industrial substitutes, 1,1,1-trichloroethane, tetrachloroethylene, and methylene chloride, was tested in vitro using the FI706 Fischer rat embryo cell system. All compounds induced transformation in the rat cells. Toxicity studies indicated that TCE was the least toxic of the four compounds by a factor of at least 100. Sc injection of transformed cells into newborn Fischer rats resulted in undifferentiated fibrosarcomas at the injection site in 100% of the animals in 27-68 days. (4 Refs) 155. HAMSTER CELLS, UNTREATED AND TREATED WITH CHEMICAL CARCINOGENS, MAINTAINED IN VITRO FOR 2 1/2 YEARS. Papadopoulo D, Levy S, Chamaillard L, Beesau O, Hubert-Habart M, Markovits P Foundation Curie-lnstitut du Radium, Section de Biologie, 26 rue d'Ulm, 75005 Paris, France Br J Cancer; 36(l).65-71 1977 The properties of untreated hamster whole embryo, fetal brain, and neonate lung cells maintained in culture for 1-2.5 yr were compared with those of cells transformed by the fol lowing chemical carcinogens: 7,10-dimethylbenzo(c)acridine, benzo(a)pyrene, 7,12-dimethylbenzo(a)anthracene, 7methylbenz(a)anthracene, 3-methylcholanthrene, and 2chiorobutadiene. Among the seven untreated lines studied, only one spontaneous transformation was observed during the first year of culture. The cells of the six other lines remained normal and diploid and were not transplantable during the first 9 to 12 mo of culture. After 12 mo, changes appeared in their in vitro behavior and their transplantability: grafts of 0.52 X 10" cells induced tumors in the hamster; fewer ceils did not. In vitro chemically transformed hamster cells were fundamentally different from untreated cells of the same origin, not only in morphological and growth characteristics but also in transplantability. Of the nine lines obtained, seven induced tumors after injection of lO'-lO* cells and two after injection of 10s cells per animal. (15 Refs) 156. MICROSOME-MEDIATED MUTAGENESIS OF A CHINESE HAMSTER CELL LINE BY VARIOUS CHEMICALS (MEETING ABSTRACT). Drevon C, Kuroki T, Montesano R International Agency for Res. on Cancer, I50cours Albert Thomas, 69008, Lyon, France Mutat Res; 53(2); IS I-182 1978 A microsome-mediated mutagenesis system for mam malian cells has been established using V79 cells, a Chinese hamster cell line. The cells, grown in monolayer, were treated with the chemicals to be tested in the presence of a postmitochondrial fraction (SI5) and cofactors for 1 hr or more (depending on the chemicals), washed and incubated for 2-3 hr on fresh culture medium, and then the cells were plated for toxicity and mutagenicity assays. Mutation was determined by resistance to 20 ug/ml 8-azaguanine or I milliM ouabain. In our assay system, S15 and cofactors were not found to be toxic to the cells. The chemicals tested included 12 nitrosamines, 3 chlorinated hydrocarbons, several polycyclic hydrocarbons and aflatoxin Bl. Dose-related mutation and cytotoxicity were induced after incubation with dimethylnitrosamine (DMN) only in the presence of both the SI5 fraction of the livers of 34 UCC 106357 BD-VI male rats and the cofactors. Pretreatment of rats with phenobarbital (PB) led to an approx a 2-fo)d increase in the mutation rate over that with the untreated rats are DMN concentrations ranging from 2 to 50 milliM, while aminoacetonitrile pretreatment reduced the mutagenic effect. Melhylcholanthrene pretreatment resulted in an increase in the mutation frequency with a higher concentration of DMN (50 milliM). A good correlation was found between in vivo carcinogenicity and this mutagenesis lest: with the exception of N-nitrosomethylphenylamine, the carcinogenic nitrosamines (DMN, N-nitrosodiethylamine, N-nitrosodi*n-propylamine, N-nitrosodi-n-butylamine, N-nitrosodi-n-pentylamine, Nnitrosomethyl-n-propylamine, N*nitrosomorpholine, Nnitrosopyrrolidin, N-nitroso-N'-methylpiperazine, Nnitrosomethylphenylamine) were mutagenic to V79 Chinese hamster cells in the presence of the PB-pretreated S15 fraction and cofactors. The non-carcinogenic N-nitrosodiphenylamine and N-nitrosomethyl-tert-butyl-amine had no mutagenic effect. Vinyl chloride, vinylidene chloride and 2chlorobutadiene were tested, but only vinyl chloride was mutagenic in the presence of PB-pretreated SI5 and cofactors while the others were found to be toxic but not mutagenic. The mutagenicity of polycyclic hydrocarbons and aflatoxin B! is now under investigation, (no Refs) F. Carcinogenicity of Vinyl Chloride and Related Compounds in Experimental Animals angiomas, skin carcinomas, hepatomas, brain neuroblastomas, and mammary carcinomas in adult SpragueDawley rats after 52 wk of exposure. Rats exposed to only 25 ppm VC for 87 wk developed HAS. Reducing the length of treatment sharply reduced the onset of several tumor types, particularly HAS: when rats were treated for 5 wk at 10.000 and 6,000 ppm, no HAS were observed. VC also had a transplacental effect: exposure of pregnant rats to 10,000 or 6,000 ppm VC on days 12-18 of pregnancy produced VCdependent tumors in the offspring. Newborn rats were also more susceptible to VC carcinogenesis than older ones. Exposure of Wistar rats to varying VC concentrations for 52 wk resulted in basically the same tumors as those produced in Sprague-Dawley rats. Exposure of Swiss mice to 50-10,000 ppm VC for 30 wk resulted in lung tumors, mammary carcinomas, HAS, vascular tumors of other types and/or sites, and epithelial tumors of the skin. In golden hamsters, the same VC concentrations resulted in HAS, skin trichoepitheliomas, melanomas, and forestomach epithelial tumors. In addition, the latency time of lymphomas was decreased from 82 wk (in controls) to 48 wk. Ingestion of 50, 16.65 or 3.33 mg/kg/day VC by Sprague-Dawley rats, 4-5 days/wk Tor 52 wk produced essentially the same spectrum of tumors as the inhalation studies. One nephroblastoma and one sc angiosarcoma were found among 240 Sprague-Dawley rats that had received one to four ip injections of 4,25 mg VC, and one nephroblastoma was found among 75 animals that had received the same dose sc. It is concluded that the neoplastic response to VC depends largely on the species and strain of animal and that age is also important with respect to tumor incidence and distribution. (5 Refs) The reader may also find the following abstracts of interest: 153. 326 157. ONCOGENIC RESPONSE OF RAT SKIN, LUNGS, AND BONES TO VINYL CHLORIDE. Viola PL, Bigotti A, Caputo A Regina Elena Inst.. Cancer Res., Rome, Italy Cancer Res; 3l(5):5l6-522 1971 Male rats were exposed to vinyl chloride vapors for 4 hr per day, 5 days per wk for 12 months; the vinyl chloride was diffused into the air of the containers which held the rats in amounts of 3% vinyl chloride v/v (30,000 ppm). Almost all the rats developed tumors of the skin and lungs. Among 17 rats surviving the 12 month course, all had skin tumors and 6 had lung tumors; 5 of the 17 had bone tumors. Most skin tumors were epidermoid carcinomas; there were 2 mucoepidermoid carcinomas and 2 epidermoid carcinomas of the keratinizing type. Among lung tumors, there were 3 adenocarcinomas, a squamous cell carcinoma and an adenocanthoma. Bone tum ors were localized in the metacarpal and metatarsal bones of all 4 extremities; all were osteochondromas. Skin tumors were localized in the area of the submaxillary and parotid glands. 158. VINYL CHLORIDE CARCINOGENICITY: AN EXPERIMENTAL MODEL FOR CARCINOGENESIS STUDIES. (PP. 119-146) Maltoni C Inst. Oncology, Bologna, Italy 40138 Incidence of Cancer in Humans, Proceedings of the Cold Spring Harbor Conferences on Cell Proliferation. Vol. 4, Hiatt HH. Watson JD, Winsten JA, ed. Cold Spring Harbor, Cold Spring Harbor Laboratory, Origins of Human Cancer., 602 pp., 1977. The carcinogenicity of vinyl chloride (VC) was evaluated in Sprague-Dawley rats, Wistar rats, Swiss mice, and golden hamsters. In inhalation studies, 50-30,000 ppm VC produced Zymbal gland carcinomas, nephroblastomas, hepatic angiosarcomas (HAS) and angiosarcomas of other sites, sc 159. PULMONARY TUMORS INDUCED IN MICE BY VINYL CHLORIDE MONOMER. Suzuki Y Environmental Science Lab., Mount Sinai Sch. Medicine, Fifth Ave. and 100th St., New York, NY, 10029 Environ Res; 16(l/3):285-30l 1978 The incidence of lung tumors due to vinyl chloride (VC) inhalation was studied in 27 CD I Charles River male mice, and precancerous changes and the resulting tumor were characterized ultrastructurally. The mice were exposed to 2,500 or 6,000 ppm VC 5 hr/day, 5 days/wk, for 5 or 6 mo and then sacrificed 2, 6, or 37 days later. Pulmonary tumors were found in 26/27 mice but in none of the 16 controls. The tumors were round, whitish, multiple, and variable in size from I to 5 mm in diameter. The tumors were arranged in tubulopapillary or adenomatous formations. There were no metastases to regional lymph nodes or other organs, parenchymal fibrosis, or fibrotic adhesions of the pleura, although occasional mitotic divisions and invaginations into the bronchiolar lumen were noted. Under the electron microscope, the characteristic features of the neoplastic cells were short microvilli, tight junctions between two adjacent cells, osmiophilic lamellar bodies, large irregularly shaped mitochondria, well-developed Golgi complexes, continuous or discontinuous basement membranes, occasional appearance of sequestration and of crystalloids, and a lack of cilia and mucous secretory granules. Some of the cells were poorly differentiated and were equipped with poorly developed organoids, without the formation of osmiophilic lamellar bodies. The gross anatomical and histological aspects of the tumors indicated that they were alveologenic tumors. The neoplastic cells were assumed to have been transformed from type II alveolar epithelium via its hyperplastic form, because of the ultrastructural similarities between normal type II cells and the neoplastic cells. It is conc luded that the mouse lung is a sensitive indicator of the oncogenicity of VC. (40 Refs) UCC 106358 35 160. INHALATION TOXICITY OF VINYL CHLORIDE AND VINYLIDENE CHLORIDE. Lee CC, Bhandari JC, Winston JM, House WB, Peters PJ, Dixon RL. Woods JS Pharmacology and Toxicology, Midwest Res. Inst.. 425 Volker Blvd., Kansas City, MO, 64110 Environ Health Perspect; 21:25-32 1977 Experiments on the inhalation toxicity and carcinogenicity of vinyl chloride (VC) and vinylidene chloride (VDC) m mice and rats are reported. The exposure of mice to 1,000 ppm VC for 6 hr/day, 5 days/wk caused some acute deaths with toxic hepatitis and marked tubular necrosis of the renal cortex. During the sixth month, mice exposed to 1,000, 250, or 50 ppm VC became lethargic, lost weight quickly, and died: only a few mice exposed to 50 ppm survived for 12 mo. In mice exposed to 50, 250, or 1,000 ppm VC, there was a high incidence of bronchioloalveolar adenoma, mammary gland tumors (including ductular adenocarcinoma), squamous and anaplastic cell carcinomas with metastasis to the lung, and hemangiosarcoma (HS) in the liver and, to a lesser extent, in other organs. The incidence and severity of these tumors were proportional to the levels and duration of exposure. Malignant lymphoma involving various organs was observed in a few mice. Rats were more resistant to the toxic effects of VC, and exposure to 1,000 ppm slightly depressed the body wt of females. Exposures of 250 and 1,000 ppm VC caused a number of deaths and hepatic HS during the 9th mo. Most rats with hepatic HS also developed HS in the lung. HS occasionally occurred in other tissues, including omentum, mesentery, or sc tissue, of rats exposed to 50, 250, or 1,000 ppm VC. The exposure of mice to 55 ppm VDC also caused a few acute deaths and a few hepatic HS. Inflammatory, degenerative, and mitotic changes occurred in the liver. No mouse exposed to VDC developed any mammary gland tumors. Several mice had a bronchioloalveolar adenoma. The exposure of rats to 55 ppm VDC slightly depressed body wt: HS occurred in the mesenteric lymph node or sc tissue of two rats. 161. C ARCINOGENICITY OF VINYL CHLORIDE AND VINYLIDENE CHLORIDE. Lee CC, Bhandari JC, Winston JM, House WB, Dixon RL, Woods JS Midwest Res. Inst., 425 Volker Blvd., Kansas City, MO, 64110 J Toxicol Environ Health: 4(1): 15-30 1978 The effects of exposure to 50, 250, or 1,000 ppm vinyl chloride (VC) or 55 ppm vinylidene chloride (VDC) for 6 hr/day, 5/days/wk, were studied in albino CD-I mice and CD rats. The animals were killed after I, 2, 3, 6, 9, or 12 mo. Bronchioloalveolar adenomas developed in 12/63, 22/63, and 48/69 mice exposed to 50, 250, and 1,000 ppm VC, respectively. Bronchioloalveolar adenomas developed in 6/35 animals exposed to VDC. Three of 29, 23/63, and 31/69 mice exposed to 50. 250, and 1.000 ppm VC. respectively, developed hemangiosarcomas (HS) in the liver. In the mice exposed to VDC. HS developed in the livers of two males and one female. Mammary gland tumors occurred in 9/34, 3/34, and 13/36 female mice exposed to 50, 250, 1,000 ppm VC respectively, and they included ductular adenocarcinomas and squamous and anaplastic cell carcinomas with metastasis to the lung. Malignant lymphomas were found in various organs of mice exposed to VC but not in any of the mice exposed to VDC. Twelve of 70 and 21/70 rats exposed to 250 and 1,000 ppm VC, respectively, developed HS in the liver; 3/34 female rats exposed to 250 ppm VC and 13/70 rats exposed to 1,000 VC also developed HS in the lung. Two of 36 male rats exposed to VDC developed HS in the mesenteric lymph node or sc tissue. It is concluded that VC is highly carcinogenic in mice; the incidence and severity of the tumors increased with dose and length of exposure. Rats were more resistant to the carcinogenic effects of VC and VDC. (26 Refs) 162. CARCINOGENICITY STUDIES ON HALOGENATED HYDROCARBONS. Weisburger EK Carcinogen Metabolism and Toxicology Branch, NCI, NIH, Bethesda, MD, 20014 Environ Health Perspect; 21:7-16 1977 The acute and chronic carcinogenicities of a series of halogenated hydrocarbons were studied in Osborne-Mendel rats and B6C3FI mice. The test chemical was given by gavage 5 days/wk for 6 wk (acute studies) or 78 wk (chronic studies). Trichloroethylene had little effect on rats but caused hepatocellular carcinomas (HC) in mice. Chloroform caused HC in mice, kidney tumors in male rats, and thyroid tumors in female rats. 1,1,1-Trichloroethane had no effect on either species, whereas iodoform produced a slight increase in thyroid tumors in male rats. 1,2-Dibromoethane and 1,2dibromo-3-chloropropane (DBCP) caused squamous cell carcinomas of the stomach with metastases in both species: DBCP also caused mammary tumors in female rats. 1,2Dichlorethane produced some stomach tumors in male rats, HC in male mice, and lung tumors in males and females of both species. 1,1-Dichloroethane had little effect on rats, but caused a slight increase in HC in male mice. 1,1,2Trichloroethane and hexachloroethane also had little effect on rats but increased the number of HC in mice, whereas 3chloropropene decreased the hepatomagenic effect in mice. Tetrachloroethylene increased the number of HC in mice but had no effect in rats. Carbon tetrachloride, used as a positive control, produced HC in rats and mice as well as thyroid tum ors in mice. The results suggest that po administration of many halogenated aliphatics might pose a carcinogenic hazard to humans on continued exposure. 163. EXPERIMENTAL STUDY OF THE CARCINOGENICITY OF CHLOROPRENE, Zil'fian VN, Fichidzhian BS, Garibian DKh, Pogosova AM Lab. Carcinogenesis, Scientific Res. Inst. Roentgenology and Oncology, Armenian SSR Ministry Public Health, Erevan, USSR Vopr Onkol: 23(4):6l-65 1977 The carcinogenicity of chloroprene (CP) was studied in male and female albino mice and rats. A rapid skin test, based on the disappearance of sebaceous glands and hair follicles upon topical application, revealed no carcinogenic activity for CP. Long-term tests of CP included topical application to mice (50% solution in benzene, 50 times), sc administration to rats (10 x 400 mg/kg or 50 x 200 mg/kg), intragastric administration to rats (50 x 200 mg/kg), and intratracheal administration to rats (5 x 200 mg/kg at 20-day intervals). None of these animals developed tumors. No tumors were found after topical application of CP (50% solution, 50 times) plus dimcthylbcnzanthracene (DMBA: 0.01% solutiqn, 5 times) but DMBA alone (0.1% solution, 50 times) induced tumors in 92% of the mice. When CP was administered sc (50 x 200 mg/kg) with DMBA (I xO.S mg),the tumor induction rate was 57.1%, but the same dose of DMBA alone induced tumors in 64% of the rats. The findings indicate that CP is not carcinogenic. (24 Refs) 36 UCC 106359 164. DETERMINATION OF THE BLASTOMOGENIC ACTIVITY OF SOME CHEMICAL SUBSTANCES BY A RAPID TEST METHOD. Garibian DKh, Papoian SA Inst. Roentgenology and Oncology, Ministry Public Health Armenian SSR, Erevan, USSR Gig Sanit; (8);74-76 1977 The possible blastomogenic effects of tobacco smoke tar, sodium dichromate, chloroprene, and vinyl acetate were studied in 870 random- bred mice aged 2-3 mo. The substances were applied to the skin in single doses or three times per week in a 30-day experiment. The blastomogenic effects were evaluated by a rapid method based on the disappearance of the sebaceous glands. Cigarette tar caused the sebaceous glands to disappear after 5-11 days, but the glands reappeared after IS days. Thickening of the skin, crust formation, progressive hy perplasia, and dystrophy of the epidermis were seen. Sodium dichromate, chloroprene, and vinyl acetate did not cause the sebaceous glands to disappear. The findings, in keeping with the results of other studies, suggest that cigarette tar is blastomogenic but that the other substances tested are not. (3 Refs) 165. CARCINOGENICITY OF TRICHLOROETHYLENE: FACT OR ARTIFACT? Henschler D, Eder E, Neudecker T, Metzler M Institut fur Toxikologie, Universitat Wurzburg, Versbacher Landstrasse 9, D-8700 Wurzburg, W. Germany Arch Toxicol (Berl); 37(3):233-236 1977 Because trichloroethylene (TCE) was previously reported to produce a high incidence of hepatocellular carcinomas in mice (but not rats) after high daily doses po. this compound was studied further. Analysis of a sample of technical grade TCE (same as that used in the bioassay experiment for carcinogenicity) by gas chromatography/mass spectrometry showed the presence of two major contaminants, epichlorohydrin and l ,2-epoxibutane, both of which were found to be highly mutagenic in the Ames test using Salmonel la typhimurium TA100. A low activity was found for trichloroethylene itself, and no activity was found for the other contaminants (diisobutylene, carbon tetrachloride, chloroform, and 1,1,1-trichloroethane). The carcinogenic effect of TCE is concluded to be due to epichlorohydrin and epoxybutane. (10 Refs) 166. TETRACHLOROETHYLENE (PERCHLOROETHYLENE). Parker JC, Bahlman U, Leidel NA, Stein HP, Thomas AW, Wolf BS. Baier EJ Natl. Inst. Occupational Safety and Health, 5600 Fishers Lane, Rockville, MD, 20857 Am Ind Hyg Assoc J; 39(3):A-23*A-29 1978 Animal and human data on the toxicity of tetrachloroethylenc (perchloroethylene) are reviewed. Force feeding of the compound to B6C3FI mice at doses of 536 or 1,072 mg/kg/day for males and 386 or 772 mg/kg/day for females for 78 wk resulted in hepatocellular carcinoma in greater than 50% of the males and 40% of the females. Studies in which the compound was inhaled did not provide sufficient data for evaluation of carcinogenicity. Tetrachloroethylenc has been shown to cause liver and kidney damage, CNS depression, skin irritation, and cardiac depression in animals: it is also teratogenic in rats and mice. Human data indicatethat it is toxic to the liver and kidneys, it is an eye irritant, and it can cause burns, blistering, and erythema of the skin; CNS depression has also been observed. This compound is usually absorbed through the lungs, but it can be absorbed from the intestines if ingested. It is deposited in the body fat and has an estimated biological half-life of 6 days in humans. It is sug gested that this compound be handled in the workplace as if it were a human carcinogen. (14 Refs) 167. OCCURRENCE OF HEPATOCELLULAR TUMORS AND HEM ANGIOSARCOMA IN RATS INHALED 1,2DIBROMOETHANE UNDER THE INFLUENCE OF D1SULF1RAM TREATMENT (MEETING ABSTRACT). Wong LC, Winston JM, Hong CB, Lee CC, Bhandari JC Pharmacology and Toxicology, Midwest Res. Inst., Kansas City. MO, 64110 Pharmacologist; 20(3); 174 1978 An 18-mo carcinogenicity study has been conducted in Sprague-Dawley rats inhaled 20 ppm of 1,2-dibromoethane (EDB) with and without 0.5% dietary disulfiram (DS) treatment. Groups of 48 males and 48 females each are treated with; (1) Air/control diet; (2) air/0.05% DS diet; (3) EDB/control; (4) EDB/0.05% DS diet. Animals are exposed to either air or 20 ppm EDB 7 hrs a day and 5 days a wk. Treatment with DS alone and the combination of EDB and DS have depressed body wt gain and food consumption of these rats. High mortality was noted during the 1st 12 mo in EDB/DS rats. The major organs affected were liver, kidney, spleen, mesentery, testes and mammary glands. Tumors developed in EDB/DS rats as early as 6 mo. Among the tum ors that occurred were hepatocellular tumors and hemangiosarcoma of the liver, spleen, mesentery, and kidneys. These results indicate that a combined EDB and DS treatment was lethal and caused high incidences of hepatocellular tumors and hemangiosarcoma. A further evaluation of the tumor incidence will be reported following the completion of the study, (no Refs) 168. REPORT ON CARCINOGENESIS BIOASSAY OF 1,2-DICHLOROETHANE (EDC). National Cancer Institute U S. Dept. Health, Education, and Welfare, NIH, NCI, Bethesda, MD, 20014 Am Ind Hyg Assoc J; 39(11):A26-A30 1978 The halogenated solvent 1,2-dichloroethane (ethylene dichloride, or EDC) was administered to rats and mice for 78 weeks at time-weighted av dosages of 95 mg/kg/day (high dose) and 47 mg/kg/day (low dose) in a carcinogenesis bioassay. In male rats, EDC caused forestomach cancers, multiple hemangiosarcomas, and subcutaneous fibromas. In female rats. EDC induced mammary cancers, appearing in some high-dose animals as early as the 20th week. EDC also caused breast and uterine cancers in female mice and respiratory tract cancers in both male and female mice, (no Refs) 169. REPORT ON BIOASSAY OF 1,1DICHLOROETHANE FOR POSSIBLE CARCINOGENICITY. Fredrickson DS NIH, Bethesda. MD, 20014 Fed Regist; 43(I43):32I90-32191 1978 1,1-Dichloroethane was administered by gavage to male and female Osborne-Mendel rats (382-950 mg/kg/day) and B6C3F1 mice (1,442-3,331 mg/kg/day) 5 days/wk for 78 wk. Rats were sacrificed 33 wk later, mice 13 wk later. Survival rates were poor in all rats and in several mouse groups. There were dose-related marginal increases in adenocarcinomas and in hemangiosarcomas among female rats and there was a statistically significant increase in the incidence of endometrial stomal polyps among female mice, (no Refs) 37 UCC 106360 170. BIOASSAY OF 1.1.2,2-TETRACHLOROETHANE FOR POSSIBLE C ARC INOGENICITY. Carcinogenesis Program National Cancer Institute National Cancer Inst.. Bethesda, MD Bioassay of 1, 1,2,2-Tetrachloroethane for Possible Carcinogenicity, Available Through National Technical Information Service, Springfield, Va, as PB-277 453/7GA. DHEW/PUB/NIH-78-827, 90 pp,, 1978. A bioassay for the possible carcinogenicity of technical grade 1. 1,2,2-tetrachloroethane was conducted using Osborne* Mendel rats and B6C3FI mice. The compound was administered in corn oil by gavage at two dose levels to groups of 30 animals of each species and sex for 78 wk. The time weighted av doses were 108 and 62 mg/kg/day for male rats, 76 and 43 mg/kg/day for female rats, and 282 and 142 mg/kg/day for all mice. Twenty animals of each species and sex were placed on test as vehicle controls and another 20 were used as untreated controls. There was a highly significant positive dose related trend in the incidence of hepatocellular carcinoma in mice of both sexes. No statistically significant incidence of neoplastic lesions was observed in male or female rats. However, two hepatocellular carcinomas and one neoplastic nodule, which are rare tumors in the male OsborneMendel rat, were observed in the high dose males. Under the conditions of this bioassay, orally administered 1,1,2,2tetrachloroethane is a liver carcinogen in B6C3F1 mice of both sexes. The results do not provide conclusive evidence for the carcinogenicity of 1,1,2,2-tetrachloroethane in OsborneMendel rats. (Author abstract) 171. INTERIM RESULTS OF TWO-YEAR TOXICOLOGICAL STUDIES IN RATS OF VINYUDENE CHLORIDE INCORPORATED IN THE DRINKING WATER OR ADMINISTERED BY REPEATED INHALATION. Rampy LW, Quasi JF, Humiston CG, Balmer MF, Schweiz BA Toxicology Res. Lab., Health and Environmental Res., Dow Chemical, U.S.A., Midland. MI, 48640 Environ Health Perspect; 21:33-43 1977 Interim results of a 2-yr toxicological study of male and female Sprague-Dawley rats exposed to vinylidene chloride (VDC) po or by inhalation are reported. Groups of 48 rats of each sex were given VDC in drinking water at mean concentrations of 0, 68, 106, and 220 ppm, corresponding to dosage levels of 0, 5.9, 10.0, and 19.3 mg/kg for male rats and 0, 7.5. I2.6,and 25.6 4 mg/kg for female rats. In the inhalation study. 86 rats of each sex were exposed to 0, 10, or 40 ppm VDC sapor for 6 hr/day, 5 days/wk,for 5 wk.after which the exposure levels were changed to 0, 25, and 75 ppm VDC. Exposure continued for a total of 18 mo, and the rats were held an additional 6 mo for observation. A separate 90-day study was made of 20 rats of each sex who were exposed to 0, 25. and 75 ppm VDC vapor. Based on gross tumor count, tumor incidence in VDC-exposed rats was not significantly greater than that in controls. Increased cytoplasmic vucuolaiion of hepatocytes was seen in the livers of rats given 200 ppm VDC in drinking water or 25 or 75 ppm VDC vapor by inhalation. 172. CARCINOGENICITY BIOASSAYS OF VINYLIDENE CHLORIDE: RESEARCH PLAN AND EARLY RESULTS. Maltoni C. Cotti G, Morisi L, Chieco P Inst. Oncology and Tumour Centre, Bologna, Italy Med Lav; 68(4):24l-262 1977 Early results (82-93 wk) of long-term carcinogenicity bioassays of vinylidene chloride (VDC) are presented. VDC was administered by inhalation to Sprague-Dawley rats (200150. 1(8), 50. 25. and 10 ppm), Swiss mice (200, 100,50, 25, and 10 ppm), and Chinese hamsters (25 ppnt) and by ingestion to rats (20, 10, 5, and 0.5 mg/kg body wt in olive oil by stomach tube). The treatment was given four to five times per week for 1 yr. In the mice, inhalation at 200, 100 and 50 ppm was discontinued after a few days because of excessive acute toxicity. In rats exposed to VDC by inhalation, the incidence of mammary tumors was higher among treated animals than in controls. No increase in mammary tumors was seen in rats given VDC by intubation. The most important result was the onset of kidney adenocarcinomas in mice exposed to 25 ppm. Male mice appeared to be more responsive than females. This tumor was not observed in mice exposed to 10 ppm or in the other treated species. No tumors were found in the hamsters. In vivo and in vitro studies indicate that VDC is probably metabolized by epoxidation into a more reactive compound that is responsible for its toxic mutagenic and carcinogenic effects. The rate at which active metabolites are formed and metabolized seems to depend on the dose of VDC as well as on the metabolic pathways of the test animals. These pathways appear to be influenced by species and sex. (15 Refs) 173. CARCINOGENICITY STUDIES ON VINYLIDENE CHLORIDE. Viola PL, Caputo A Regina Elena Inst. Cancer Res., Rome. Italy Environ Health Perspect; 21:45-47 1978 The possible carcinogenicity of vinylidene chloride (VDC) was determined in two experiments, one using Wistar rats, one using Sprague-Dawley rats. The Wistar rats inhaled VDC for 4 hr/day, 5 days/wk, for 12 mo at a concentration of 200 ppm for 5 mo, 100 ppm thereafter. The animals were allowed to die spontaneously (life-span was 22-24 mo). Tumors, all occurring in the abdominal cavity except for one in the liver and one in the lung, were found in 8/51 males and 9/23 females. The diagnosis was reticulum cell sarcomas of a nonsyncytia! type. However, many abdominal tumors were also found in control rats (5/30 males, 10/30 females). This prompted the second experiment, in which Sprague-Dawley rats were exposed to 75 or 100 ppm. Tumor incidence in the controls was practically identical to that found in the 100 ppm group and higher than that in the 75 ppm group. Microscopic examination of the tissues and organs from all animals is incomplete. Nevertheless, it seems clear that there is no grossly observable correlation between tumor formation and VDC inhalation. (7 Refs) 174. BIOASSAY OF 1.1,1-TRICHLOROETHANE FOR POSSIBLE CARCINOGENICITY (MEETING ABSTRACT). Author not identified NCI. Bethesda, MD Gov Rep Announce Index: 77(13): 111 1977 A bioassay for the possible carcinogenicity of technical grade 1,1.1-trichloroethane was conducted using OsborneMendel rats and B6C3FI mice. The 1,1.1-trichloroethane was administered po by gavage in corn oil to 50 animals of each sex and species at two dose levels 5 days/wk for 78 wk. The rats received two doses of 1500 and 750 mg/kg, which produced a moderate depression of body wt in the 1st yr of the study. A yellow discoloration of lower abdominal fur, eye and nasal discharge and dyspnea were observed during the 2nd yr. Both male and female test animals were killed at 117 wk of age. In mice, the doses were increased twice and the time-weighted av doses were 5.616 and 2.807 mg/kg. There was a moderate depression of body wt throughout the study in both sexes, and survival was significantly decreased. Neoplasms encountered In both the treated and control animals had been seen previously in untreated animals. The neoplasms were not believed to be attributable to 1,1.1-trichloroethane since no relationship was established between the dosage groups, species, sex. type of neoplasm or site of occurrence. UCC 106361 175. CARCINOGENIC ACTIVITY OF DI- AND TRIFUNCTIONAL .ALPHA.-CHLORO ETHERS AND OF I. 4-DICHLOROBUTENE-2 IN ICR/HA SWISS MICE. Van DUUREN BL. Goldschmidt BM. Seidman I Laboratory of Organic Chemistry and Carcinogenesis, Institute of Environmental Medicine, New York University Medical Center, New York, New York 10016 Cancer Res: 35(9):2553-2557 1975 Four bifunctional and one trifunctional alpha-chloro ethers were tested for carcinogenicity. These compounds were bis-1,2-(chloromethoxy)ethane (Compound I), bis-1,4(chloromethoxy)butane (Compound II), bis-l,6-(chloromethoxy)hexan (Compound III), bis-l,4-(chloromethoxy)-pxylene (Compound IV), and tris-1,2,3-(chloromethoxy)propane (Compound V), Trans-1,4-Dichlorobutene2 (Compound VI) was tested along with the five alpha-chloro ethers. All six compounds were tested in female ICR/Ha Swiss mice for 502 to 569 days, depending on survival, by skin ap plication or sc and ip injection. There were 30 or 50 micc/goup The ip and sc injections were given once weekly at 0.1 or 0.3 mg of compound dissolved in 0.05 ml tricaprylin for Compounds 1 to V arid 0.05 mg/0.05 ml tricaprylin for Comp ound Vi for the duration of the tests. The skin applications, three limes weekly, were at doses of 0.3 or 1.0 mg/0.1 ml cyc lohexane for the alpha-chloro ethers and 1.0 mg/0.1 ml acetone for Compound VI. Vehicle and no treatment controls were carried out together with the test compounds. Significance values (p) were calculated for all the compounds tested. Three compounds, I, IV and V, gave notable tumor incidences by all three routes of administration. Compounds II. Ill, and VI were either inactive by one or more routes of administration or gave low tumor yields. (Author Abstract) 176. TRANS-l,4-DICHLOROBUTENE. (PP. 149-154) IARC Working Group I ARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Man: Some Fumigants, the Herbicides 2,4-D and 2,4,5-T, Chlorinated Dibenzodioxins and Miscellaneous Industrial Chemicals. Lyon, International Agency for Research on Cancer, Vol 15, 1977. The carcinogenicity of trans-l,4-dichlorobutcne has been tested in ICR/Ha Swiss mice given the compound po, sc and ip. Low incidences of local sarcomas were noted in the sc and ip studies, but these results do not permit conclusive evaluation of carcinogenicity. No human epidemiological data were available for review. (II Refs) 177. CARCINOGENICITY AND CHEMICAL REACTION WITH GUANINE OF l-CHLOROPROPENE AND TWO OF ITS POTENTIAL METABOLITES (MEETING ABSTRACT). Goldschmidt BM, Van Duurcn BL, Goldstein RC, Smith AC Lab. Organic Chemistry, Inst. Environmental Medicine, New York Univ. Medical Center, New York, NY, 10016 Proc Am Assoc Cancer Res: 20:90 1979 l-Chloropropene. the simplest homologue of the human carcinogen vinyl chloride, along with two of its potential metabolites. I-chloro-1,2-epoxypropane and 2chloropropanal, were tested for carcinogenicity in female ICR/Ha Swiss mice (30/group). Repeated skin application of the alkene or aldehyde, and single dose initiation of the three compounds followed by promotion with phorbol myristate acetate failed to yield skin tumors. However, weekly sc injection of the aldehyde (1.0 mg) yielded 4 local sarcomas (P less than 0.005). Weekly intragastric feeding of the alkene (1.0 mg) led to 10 forestomach papillomas and 3 carcinomas (P less than 0.0005) while the aldehyde (1.0 mg) led to 6 forestomach papillomas (P less than 0.05). Upon being allowed to react with guanine in dimethylsulfoxide the alkene failed to react, while the epoxide and aldehyde yielded the identical new compound, C8H8CIN50. Based on UV and nuclear magnetic resonance spectra and other data the structure of the comp ound was established. It is an enamine formed by reaction at the 2-amino group of guanine. It should be noted that the potent carcinogenic metabolites of benzo(a)pyrene react predominantly with the 2-amino group of guanine in polynucleotides, just as the potential metabolites of Ichloropropene did in our study, (no Refs) 178. MOUSE SKIN CARCINOGENICITY TESTS OF THE FLAME RETARDANTS TRIS(2,3DIBROMOPROPYDPHOSPHATE, TETRAKIS(HYDROXYMETHYL)PHOSPHONIUM CHLORIDE, AND POLYVINYL BROMIDE. Van Duuren BL, Loewengart G, Seidman I, Smith AC, Melchionne S Lab. Organic Chemistry and Carcinogenesis, Inst. Environmental Medicine, New York, NY. 10016 Cancer Res; 38(l0):3236-3240 1978 The flame retardants tris(2,3-dibromopropyl)phosphate (iris), tetrakis(hydroxymethyi)phosphonium chloride (THPC), and polyvinyl bromide (PVB) were tested for carcinogenic activity by thrice weekly skin application in female Icr/Ha Swiss mice for 420-496 days. Tris, at doses of 30 or 10 mg/ap plication (30 mice/group), induced benign and malignant tum ors of the skin, forestomach, and oral cavity (tongue and gingiva) in a statistically significant number of mice (p less than 0.0005). A statistically significant incidence of papillary tumors of the lung was observed at both doses (p less than 0.0005). One carcinoma of the liver was observed at both doses,, and the higher dose also resulted in a tubular adenocarcinoma of the kidney in one mouse. THPC (2 mg/ap plication, 60 mice) and PVB (0.1 ml latex suspension/ap plication, 30 mice) were inactive. PVB was also injected sc (ap prox 23 mg) into the mice once weekly for 48 wk; the mice were observed for an additional 12 wk. Liposarcomas were induced in 19/30 mice; the tumors were ascribed to physical carcinogenesis because the aqueous suspension contained 90% solids. The internal tumors observed in the PVB-treated animals were not significantly different from those observed in controls. (28 Refs) 179. INDUCTION OF STOMACH CANCER IN RATS AND MICE BY HALOGENATED ALIPHATIC FUMIGANTS. Olson WA, Habermann RT, Weisburgu EK, Ward JM, Weisburger JH Hazelton Labs., Inc., Vienna, Va. J Natl Cancer Inst; 51(6): 1993-1995 1973 Ethylene dibromide (EDB) and l,2-dibromo-3chloropropane (DBCP) were administered to Osborne-Mendel rats and (C57BL X C3H)FI mice via chronic oral intubation five times per wk at experimentally predetermined maximally tolerated doses and at half those doses. Fifty animals of each sex were used for each dose level-a total or 200 rats and 200 mice for each compound. As early as 10 wk after initiation of treatment, both compounds induced a high incidence of squamous cell carcinomas of the stomach in both species. In addition, DBCP induced mammary adenocarcinomas in the female rats. These results are most pertinent to agricultural and food storage workers who disperse these volatile materials through soil or food. Although the hazard to any such workers would probably he largely through inhalation and not through oral exposure as was done in this study, anyone exposed to DBCP or EDB should take protective measures. 39 UCC 106362 180. BIOASSAY OF DIBROMOCHLOROPROPANE FOR POSSIBLE CARCINOGENICITY. C AS NO. 1836-75-5. National Cancer Institute Carcinogenesis Program Carcinogenesis Program. NCI, Bethesda, MD Bioassay of Dibromochloropropane for Possible Carcinogenicity, CAS No. 1836-75-5, Available Through National Technical Information Service, Springfield, Va., as PB-277 472/7GA., NCI-CG-TR-28, DHfcW, PUB/NIH-7S-828. 93 pp,, 1977. \ hioassay for the possible carcinogenicity of technical grade dibromochloropropane (DBCP) was conducted using Osbomc-Mcndel rats and B6C3FI mice. The compound was administered in corn oil by gavage at two doses for varying lengths of time ranging from 47-78 wk. Time weighted av doses of DBCP were 29 mg/kg/day for high-dose rats: and 15 mg kg day for low-dose rats. The time-weighted av concentrations for the high-dose male and female mice were 219 and 209 mg/kg/day, respectively. For low-dose male and female mice the concentrations were 114 and 110 mg/kg/day, respectively. Fifty animals of each species and sex served as vehicle (corn oil) controls. Twenty animals of each species and se\ also served as untreated controls. In rats and mice of both sexes, there was a statistically significant incidence of forestomach squamous cell carcinomas. In female rats the incidence of adenocarcinoma of the mammary gland was statistically increased. Toxic nephropathy was observed in all treated animals. Under the conditions of this bioassay, DBCP is a stomach carcinogen in rats and mice of both sexes and is carcinogenic to the mammary gland in female rats. (Author abstract) 181. RESULTS OF A TWO YEAR CHRONIC TOXICITY SH I)) W ITH HEXAC HI.OROBLTADIENE RATS. Kociba RJ, Keyes DG, Jersey GC, Bullard JJ, Dittenber DA. Quasi JF, Wade CE, Humiston CG, Schweiz BA toxicology Res, Lab.. Health and Environmental Res., Dow Chemical U.S.A., Midland, Ml 48640 \m Ind Hyg Assoc J; 38(11):589-602 1977 The possible toxicological effects associated with the chronic ingestion of hexachlorobutadiene (HCBD) in the diet of rats were investigated. Male and female Sprague-Dawley rats were maintained for up to 2 yr on diets containing 0,2, 2.0, or 20 mg kg day. The kidney was the primary organ affected by the toxin. Ingestion of 20 mg/kg/day of HCBD for up to 2 yr caused decreased body wt gain and survival, increased the urinary excretion of coproporphyrin, increased kidney wt, increased renal tubular hyperplasia/proliferation and renal tubular adenomas and adenocarcinomas, some of which metastasized to the lungs. Lesser degrees of toxicity were observed in the rats receiving 2mg/kg/day. They had increased urinary coproporphyrin excretion, and an increase m renal tubular epithelial hyperplasia/proliferation. No neoplasms were found in these rats. No discernible ill effects were observed in the rats receiving 0,2 mg/kg/day for up to 2 yr. Thus, a clear-cut dose-response relationship for HCBDiuduccd toxicity was observed in which the HCBD-induced neoplasms occurred at a level higher than that causing discernible renal injury. Irreversible toxicologic effects occurred at a dosage level which caused significant tissue iiiiury and other manifestations of toxicity. No neoplasms occurred at dosage levels which caused no injury or only minor miury that was reversible. (10 Refs) 182. MUTACENJC AND CARCINOGENIC EFFECTS OF VIN\ L CHLORIDE. Bartsch H, Montesano R International Agency for Res. on Cancer, Unit Chemical Carcinogenesis, 150 cours Albert Thomas, 69008 Lyon, France Mutat Res: 32(2):93-l 13 1975 The carcinogenic and mutagenic effects of vinyl chloride (VC) are described. VC administered bv inhalation is carcinogenic in rats, mice, and hamsters, producing angiosarcoma of the liver and other tissues in all three. In ad dition, in rats, VC induces tumors of the Zvmbal glands and of the skin, nephroblastomas, hepatomas, and neuroblastomas. In mice, tumors of the lung and skin are also observed. The mutagenic activity of VC has been demonstrated by an in vitro technique utilizing the reverse mutation system of Salmonella typhimurium, in which the genetic indicator reverts to histidine prototrophy by single base-pair substitutions or by base-pair insertions or deletions. The carcinogenicity of VC in man and animals and its mutagenic action after metabolic conversion by microsomal enzymes from humans and rodents into electrophilic derivatives strengthen the relationship between mutagenesis and carcinogenesis. Despite the fact that there is no direct proof that chloroethylene oxide is the metabolite of VC primarily responsible for its biological effects in vivo, the biological importance of this compound is stressed by circumstantial evidence. The available data concerning the biological hazards of VC demonstrate that it is toxic, carcinogenic, and mutagenic in animals and man. (100 refs) 183. INDUCTION OF PULMONARY ADENOMAS IN STRAIN A MICE BY SUBSTITUTED ORGANOHALIDES. Theiss JC. Shimkin MB. Poirer LA Dept. Community Medicine, Sch. Medicine, Univ. California, La Jolla. CA, 92093 Cancer Res; 39(2.Partl):39l-395 1979 An investigation was made of the abilities of 28 organohalides (10 monochlorinated and 18 monobrominated derivatives of alcohols, esters, ethers, carboxylic acids, ketones, and amines that contained 2-4 carbon atoms) to induce lung adenomas in strain A mice. Groups of 20 mice (10 males. 10 females) received ip injections 3x/wk of either the max tolerated dose (MTD), 0.5 MTD, 0.25 MTD, or 0.20 MTD. Except for the more toxic compounds. 24 injections were given when possible. The mice were sacrificed 24 wk after the first injection and their lungs examined. 2-Chloro-N,Ndimethylethylamine hydrochloride, 3-chloropropionic acid, 4chloro-1-butanol, and 2-bromoethanol produced an elevated pulmonary adenoma response that was significant by two different statistical tests. These substituted derivatives ap peared to exert tumorigenic activity at lower doses than those of similar unsubstituted compounds (previous data): thus, nucleophilic substitution may increase the tumorigenicity of linear alkyl halides. Ethyl chloroacetate, 3-chloropropene, 3chlorobutvric acid, 3-bromopropionic acid, and 3bromopropylamine hydrobromide produced an elevated lung tumor response that was significant by only one of the statistical tests used. These compounds thus had borderline tumorigenicity in this bioassay. Four of the organochlondes and 15 of the organobromides showed no significant tum origenicity. Among the negative compounds were three Deviating agents, isobutyryl bromide, butyryl bromide, and benzoyl bromide. In general, the MTD's of the organo- 40 UCC 106363 bromides were substantially lower than those of the organochlorides The finding that a greater percentage of the chloro compounds showed tumorigenicity than did the bromo derivatives was unexpected, based on chemical activity and on previous studies of mutagenicity and carcinogenicity among organohalides, The greater toxicity of the organobromides toward strain A mice may mask their potential tumorigenicity in some cases, as these compounds could not be administered in sufficient amounts to induce tumor formation. (14 Refs) G. Factors Modifying Carcinogenicity and Toxicity of Vinyl Chloride and Related Compounds 184. EFFECT OF ETHANOL AND VINYL CHLORIDE ON THE INDUCTION OF LIVER TUMORS: PRELIMINARY REPORT. Radike MJ, Stemmer KL, Brown PG, Larson E, Bingham E Univ. Cincinnati, Inst. Environmental Health, Kettering Lab., Cincinnati, OH, 45267 Environ Health Perspect; 21:153-155 1977 A preliminary report of the effect of ethanol on vinyl chloride (VC) carcinogenicity in 320 male Sprague-Dawley rats is presented. Equal numbers of rats were divided into four groups. Group 1 received a normal diet and breathed filtered air for life. Group 2 received a normal diet plus 5% ethanol in the drinking water and breathed filtered air for life. Group 3 received a normal diet but breathed 600 ppm VC 4 hr/day, 5 days/wk, for 1 yr. Group 4 received a normal diet plus ethanol starting 4 wk before VC exposure. The ethanol exposure lasted until the animal died or was sacrificed. Sixty weeks after the first exposure to VC, 55 rats had died or had been sacrificed. No tumors were noted in eight Group I animals that had died. Of six dead Group 2 animals, one had a kidney tumor. Of 13 dead Group 3 animals, 5 had liver tumors (2 of them being angiosarcomas) and 2 had lung tumors. Of 28 dead Group 4 animals. 21 had 26 liver tumors (5 angiosarcomas) and 2 had kidney tq,mors. These preliminary findings suggest a synergism between VC inhalation and ingested alcohol in tumorigenesis. (6 Refs) 185. EFFECT OF VARIOUS TREATMENTS ON TOXICITY OF INHALED VINYLIDENE CHLORIDE. Short RD, Winston JM, Minor JL, Seifter J, Lee CC Pharmacology and Toxicology, Midwest Res. Inst., Kansas City, MO. 64110 Environ Health Perspect; 21:125-129 1977 The acute toxicity of continuously inhaled vinylidene chloride (VDC) was investigated in CD-I mice and CD rats, and the effects of various treatments on this toxicity were determined. The animals were exposed to varying VDC concentrations for 22*23 hr/day. VDC was more lethal and more hepatotoxic mice than male rats. Furthermore, male mice were more sensitive to the lethal effects of VDC than females; the respective concentrations causing 50% mortality were 98 and 105 ppm. The effects of disulfiram, diethyldithiocarbamate, thiram. cysteine, methionine, Nacetylcysteine, SKF 525-A, cobaltous chloride, dimercaprol, phenoxybcnzamine. propranolol, vitamin C, and DL-alphatocopherol acetate on VDC toxicity were evaluated. Disulfiram, diethyldithiocarbamate and thiram reduced the acute lethal and hepatoxic effects of inhaled VDC. Disulfiram also reduced the levels of covalently bound radioactivity in the liver and kidney following ip administration of ,4C-VDC. It is suggested that disulfiram and its metabolites act by not only reducing the activation of VDC, but also by increasing the extent of detoxification. (25 Refs) 186. EVALUATION OF A POSSIBLE ROLE FOR ANTIMUTAGENS, ANTITERATOGENS, AND ANTICARCINOGENS IN REDUCING ENVIRONMENTAL HEALTH HAZARDS. Nashed N Feuerbackstrasse 3. 6 Frankfurt am Main, W, Germany Environ Health Perspect: 14:193-200 1976 The use of protective agents such as antibiotics, cations, vitamins, sulfhydryl compounds, and amino acids offers a means of dealing with the burden of environmental health haz ards facing man. Dietary supplements of appropriate protective agents may protect people working with genetically active agents. Among these are workers in oil refineries and in the manufacture of asbestos, certain pesticides, polyvinyl chloride, and alkylating agents. The use of vitamin C as a preventive measure against nitrosamines in food is only justified if its presence does not also protect bacteria against the antibiotic effect of nitrite. Reducdyn (a combination of Nacetyihemocysteine-thilactone, L-cysteine, and fructose) is already being successfully used against the adverse effects of irradiation and of the clastogenic alkylating agent trenimon. L-Cysteine has been shown to have anticarcinogenic, antimutagenic, and antiteratogenic effects. It should be possible, once the mechanisms of action of both inducers and protectors are known, to be able to protect against a given inducer by selecting the optimal protector and conditions most suited to counter its action. Efforts should be intensified to gain a better understanding of protection mechanisms by using model systems. (71 refs) 187. 1,1-DlCHLOROETHYLENE HEPATOTOXICITV : EFFECT OF ALTERED THYROID FUNCTION AND EVIDENCE FOR THE SUBCELLULAR SITE OF INJURY. Jaeger RJ, Szabo S, Coffman LJ Dept. Physiology, Harvard Sch. Public Health, 665 Huntington Ave., Boston, MA 02115 J Toxicol Environ Health; 3(3):545-555 1977 To study the effect of altered thyroid function on the hepatotoxicity of 1,1-dichloroethylene (l.l-DCE), male Sprague-Dawley rats were exposed for 4 hr to l.l-DCE by inhalation following an 18 hr fast. The rats were sacrificed 6 hr after exposure. Under these conditions thyroidectomy provided significant protection against the toxic effect of 1,1DCE. On the other hand, thyroxine pretreatment significantly potentiated the toxicity of this chemical. The thyroidectomized animals had higher values of liver glutathione (GSH) than control animals. Thyroxine pretreatment resulted in a significant reduction in GSH concentration. The antithyroidal agents propylthiouracil and methimazole were comparable to thyroidectomy in their protection against the hepatotoxic efect of l.l-DCE. The two pretreatments were also associated with significant elevations in liver GSH concentration, showing that decreased thyroid function was associated with increased liver sulfhydryl content. Subcellular fractionation of livers from fed or fasted rats exposed to air or l.l-DCE showed that mitochondrial GSH concentrations were reduced to a greater extent than those of other fractions. Decreased oxygen uptake by liver homogenate appeared to precede changes in serum alanine-alpha-ketoglutarate transaminase (AKT). Serum sorbitol dehydrogenase, a cytoplasmic marker, was positively correlated with changes in scrum ornithine carbamoyl tranaminase, a mitochondrial marker, indicating that mitochondrial damage may occur prior to or simultaneously with cytoplasmic membrane rupture. The results suggest that the thyroid gland regulates the concentration of glutathione in the liver and support a previously described hypothesis. A mechanism for the action of l.l-DCE is proposed. (17 Refs) 41 UCC 106364 188. ORAL TOXICITY OF 1,1-DICHLOROETHYLENE IN THE RAT: EFFECTS OF SEX, AGE, AND FASTING. Andersen ME, Jenkins LJ Toxicology Detachment, Naval Medical Res. Inst., Wright- Patterson Air Force Base, OH, 45433 Environ Health Perspect; 21:157*163 1977 The acute po toxicity of 1,1-dichloroethylene (1,1-DCE; vinylidene chloride) was investigated in male and female Holtzmun HOT:(SD)BR rats of various sizes. When fasted and fed large, mature, male rats were given 400 mg 1,1-DCE, the hepatotoxic response was four to five times greater in the fasted rats. Determinations of the po LD50 of male rats indicated that for large animals (395 g), mortality increased monotonically from 0% to 100% as the dose increased from 800 to 2,000 mg/kg: the estimated acute LD50 was similar to that reported previously, 1,550 mg/kg. With males weighing 224 g, mortality varied between 10% and 33% following doses of 50-800 mg/kg 1,1-DCE. With rats weighing 73 g, 100% mortality was noted at 300 mg/kg; percent mortality then decreased as the dose was increased up to 800 mg/kg. Maxima and extended plateaus were seen in the survival curves of all males between doses of 100 and 700 mg/kg, making it impossible to calculate an exact LD50. Following a dose of 50 mg/kg, mortality and hepatotoxicity were greatest in rats weighing 100-150 g; smaller and larger males were less susceptible. Female rats did not show this variation in susceptibility with size, and they were less susceptible to the toxic effects of 1,1-DCE: the threshold of toxicity in male rats of 100-150 g occurred near 50 mg/kg. while for females it was closer to 100 mg/kg. These findings suggest that 1,1-DCE is metabolized to a toxic intermediate by some saturable pathway. Based on the effects of pretreatment with microsomal enzyme inhibitors and activators on 1,1-DCE toxicity in rats of various sizes, it appears that there are at least two microsomal reactions involved in 1,!*DCE metabolism. (25 Refs) 189. ENHANCEMENT OF 1,1-DICHLOROETHYLENE TOXICITY BY PRETREATMENT OF FASTED MALE RATS WITH 2,3-EPOXYPROPAN-l-OL. Andersen ME, Jones RA, Jenkins LJ Naval Medical Res. Inst., Toxicology Detachment, Building 433 Area B. Wright-Patterson Air Force Base, OH 45433 Drug Chem Toxic; l(l):63-74 1977 The enhancement of 1,1-dichloroethylene (1,1-DCE) toxicity by pretreatment with 2,3-epoxypropan-l-ol (EP) was studied in fasted male Holtzman rats. Pretreatment with 278 mg EP/kg reduced the acute LD50 of 1,1-DCE by a factor of five (to less than 40 mg/kg). Low mol wt epoxides appear to increase the toxicity of 1,1-DCE by interfering with the metabolism of a toxic product of the microsomal oxidation of 1,1-DCE. Other compounds tested showed lower enhancement effects than EP, including styrene oxide, trichloropropanc-2,3-oxide, cyclohexene oxide, diethylmuleate. and butadiene monoxide. (18 Refs) 190. POSSIBLE METABOLIC INTERACTION BETWEEN STYRENE AND ORGANIC SOLVENTS (MEETING ABSTRACT). (PP. 10) Ikeda M, Hirayama T Dept. Environmental Health, Tohoku Univ. Sch. Medicine, Scdnai 980. Japan Proceedings of the International Symposium on Styrene; Occupational and Toxicological Aspects Held by the Institute of Occupational Health in Helsinki (Finland), 17-19 April, 1978. Institute of Occupational Health, Helsinki. Finland. 56 pp., 1978. Animal experiments were initiated to examine the possibility that ceexposure to organic solvents affects the metabolism of styrene and modifies its toxicity. Male Wistar rats (about 300 g) were given styrene ip (2.2 moles/kg) disso lved in soybean oil, together with n-hexane, ethyl acetate, methylchloroform, acetone, trichloroethylene or toluene. Phenylglyoxylic and mandelic acid were measured in 24-hr urine samples. A preliminary experiment revealed that most of the metabolites are excreted in urine during the time period studied. The amounts of the urinary metabolites were reduced by about 10% when an equimolar amount of either trichloroethylene or toluene was administered in conjuction with styrene, while the other solvents were essentially ineffective. With a trichloroethylene dose of 11 moles/kg (5 x as much a styrene), styrene metabolism was suppressed by 4449%; a suppression of 45-46% was observed with 11 mole of to luene/kg. In parallel experiments, the metabolism of benzene after ip injection was suppressed by coadministered toluene, and this observation could be reproduced in mixed vapor exposure experiments as well. Accordingly, inhalation experiments are in progress to confirm the suppressive effect of trichloroethylene and toluene on the metabolism of styrene after vapor exposure, (no Refs) IV. STRUCTURE-ACTIVITY RELATIONSHIPS FOR VINYL CHLORIDE AND RELATED COMP OUNDS The reader may also find the following abstracts of interest; 38, 40. 43, 50. 56. 58, 59, 65. 69. 109, 117. 133, 140 191. ALKYLATING AND MUTAGENIC EFFECTS OF ALLYL AND ALLYLOGENIC COMPOUNDS (MEETING ABSTRACT). Eder E, Neudecker T Dept. Toxicology, Univ. Wurzburg, Versbacher Landstrasse 9, D-8700 Wurzburg, W. Germany Naunyn Schmiedebergs Arch Pharmakol; 302(Suppl):R2l 1978 Some ally! compounds have been found active, others inactive in carcinogenicity testing in intact animals. The carcinogenic activity has been attributed, up to now, to the possible epoxidation of the olefinic moiety in these molecules. However, allylic or allylogenic structures are generally characterized by a rather strong SSUBN-I as well as SSUBN-2 reactivity; they may alkylate nucleophilic biomolecules and thus initiate chemical carcinogenesis and mutagenesis. We investigated the direct alkylating properties of a series of allyl and allylogenic compounds and tested their mutagenic potential in an in vitro-bactcrial testing system (A). The selection of compounds was guided by theoretical evaluation of the influence of various substituents on the reactivity of the molecules. These alkylating properties were determined by reaction with 4-nitrobenzyl pyridine (B). Positive results in A and B were found with: allyl chloride, allyl cyanide, l-chloro2-methyl-2-propcne, l-chloro-2-butene; negative, in both tests (A and B) were allyl amine and diallyl sulfide. With allyl alc ohol. a high mutagenic potential (A) did not correlate with a negative result in B; this, however, might be due to the fact that OH. under the condition of test, does not represent a good leaving group. These findings clearly indicate the possibility that allyl compounds might exert a direct carcinogenic effect. 42 UCC 106365 192. STRUCTURAL PROGNOSTICATION OF CARCINOGENICITY AND TUMOR-ENHANCING ACTIVITY IN VARIOUS CHEMICALS. (PP. 2071-2084) Van Duuren BL Lab. Organic Chemistry, New York Univ. Medical Center, New York, NY, 10016 Prevention and Detection of Cancer, Proceedings of the Third International Symposium on Detection and Prevention of Cancer Held by the International Study Group for the Detection and Prevention of Cancer in New York, April 26 - May I, 1976. Vol. 2(Part I), International Study Group for the Detection and Prevention of Cancer, New York, NY, 2404 pp., 1978. Structure-activity relationships of direct-acting alkylating agents, tumor promoters, and cocarcinogens are reviewed. Among the 100 alkylating agents examined were epoxides (mono-, bi- and polyfunctional), beta- and gamma-lactones, and a variety of chloro ethers. The beta-lactones are carcinogenic but the gamma-lactones are not. The bifunctional epoxides exhibit carcinogenicity more frequently than the monofunctional analogs. In monofunctional agents, the presence of a reactive adjacent functional group results in carcinogenic activity. Trichloroethylene (TCE) and vinyl chloride (VC) inay be metabolized via an epoxide intermediate. TCE is carcinogenic in mice but not in rats, and VC is a known human carcinogen. Because of the wide differences in chemical structure, reactivity, and physical properties of tumor promoters, cocarcinogens, and tumor inhibitors, they probably exert their activities in several different ways. They may simply alter the rate of absorption and disappearance of a carcinogen, or they may alter its metabolic pathway. Tumor-promoting agents, particularly the phorbol esters, are likely to interact at the cell membrane. As a result of structure-activity studies, it has become possible to assign biological activity to certain classes of compounds that have not yet been tested. (41 Refs) examination of chemical structure, reactivity, stereochemistry and known or suspected metabolic pathways can now be used much more effectively in pinpointing potential carcinogens in the environment. (Author Abstract) 194. CHEMICAL STRUCTURE, REACTIVITY, AND CARCINOGENICITY OF HALOHYDROCARBONS. Van Duuren BL Lab. of Organic Chemistry and Carcinogenesis, Inst, of Environmental Medicine, New York Univ. Medical Center, New York, NY, 10016 Environ Health Perspect; 21:17-23 1977 The structural and carcinogenic properties of halohydrocarbons are reviewed, with particular emphasis on trichloroethylene (TCE). Based on studies of the chemical structure, reactivity, and possible metabolic pathways of TCE, the compound was predicted to be carcinogenic, particularly to the liver. These studies also suggested that TCE is metabolized to an epoxide and that this epoxide may be the activated carcinogenic intermediate of TCE in liver carcinogenesis. The binding of TCE to liver microsomal proteins of male B6C3FI hybrid mice, which are susceptible to TCE-induced liver tumorigenesis, was found to be significantly higher than the binding of TCE to microsomal proteins of male Osborne-Mendel rats, which are resistant to TCE-induced hepatocellular carcinoma. Also, the in vitro binding of TCE to liver microsomal proteins was higher for male than female B6C3F1 mice; females have been reported to show a lower incidence of TCE-induced hepatocellular carcinoma than males. The results of carcinogenicity assays of vinyl bromide and polyvinyl bromide are also given. Neither compound appeared to be carcinogenic when injected sc or when applied to the skin of mice. The carcinogenicity of other chlorinated hydrocarbons that are widely used in the chemical industry and that are structurally analogous to TCE and vinyl chloride is discussed briefly. 193. STRUCTURAL PROGNOSTICATION OF CARCINOGENICITY AND TUMOR ENHANCING ACTIVITY IN VARIOUS CHEMICALS (MEETING ABSTRACT). (PP. 130-131) Van Duuren BL Lab Organic Chem and Carcinogen, Inst Environ Med, NY Univ Med Ctr, NY. NY 20016 Third International Symposium On Detection And Prevention Of Cancer. 1976. The chemical reactivity and carcinogenicity of epoxides, lactones, their N and S isosteres and haloethers were examined. The findings are important with regard to mode of action of direct-acting carcinogens, and more immediately with regard to environmental carcinogenesis. An example is bis(chloromethyl)ether which was pinpointed in a long list of potential occupational carcinogens and was then shown to be carcinogenic in mice and rats. Subsequent epidemiologic studies in chemical workers led to its designation as a human carcinogen. Thus, the usual pattern of uncovering occupational carcinogens, ie from epidemiologic studies, has been reversed in this instance. It was also suggested that trichloroethylene will be carcinogenic particularly to the liver. Subsequently the National Cancer Institute announced that it caused liver cancer in mice. Structural changes in the tumorpromoting agent phorbol myristate acetate have been made and the products were examined for tumor-promoting activity on mouse skin. Phorbol and phorbol triacetate are inactive; phorbolol myristate acetate showed decreased activity. Longchain aliphatic hydrocarbons (CIO -CI6) and the phenols catechol, resorcinol, hydroquinone and pyrogallol show remarkable differences in cocarcinogenicity. Close 195. METABOLISM AND MUTAGENICITY OF HALOGENATED OLEFINS - A COMPARISON OF STRUCTURE AND ACTIVITY. Henschler D Institut fur Toxikologie und Pharmakologie, Universitat Wurzburg, D-87QO Wurzburg, W. Germany Environ Health Perspect; 21:61-64 1977 The metabolism of various halogenated olefins was studied in vivo, and their mutagenicity was investigated in vitro in a modified Ames testing system. The results of the mutagenicity test were compared with in vivo carcinogenicity Endings reported previously. In mammals, chlorinated ethylenes are first metabolized to epoxides, which may then undergo intramolecular rearrangement. This reaction has been studied in the entire series of chlorinated epoxyethanes. The rearrangement products found were acyl chlorides (tetrachloro-, trichloro-, and 1,1-dichloroethylenes) or chlorinated aldehydes (cis- and trans-!,2-dich!oroethylene. vinyl chloride). In vivo experiments yielded products that were further derivatives of these rearrangement products, except that with trichloroethylene, the only rearrangement product was chloral. Tetrachloroethylene, trichloroethylene, cis-1,2dichloroethylene, trans-l,2-dichloroethylene, 1,1dichloroethylene. and vinyl chloride were then tested for mutagenicity in the Salmonella typhimurium assay. Vinyl chloride, trichloroethylene, and 1,1-dichloroethylene were mutagenic, suggesting that asymmetric chlorine substitution 43 UCC 106366 renders the epoxides unstable and mutagenic. High electrophilicity may be a prerequisite for the mutagenic and carcinogenic activity of these chlorinated ethylenes. (20 Refs) 196. MOLECULAR ASPECTS TO THE OXIRANEFORMATION OF TRICHLOROETHYLENE AND OTHER CHLORINATED ETHYLENES (MEETING ABSTRACT). Bonse G. Henschler D Inst, Toxicology, Univ. Wurzburg, D 8700 Wurzburg. Versbacher Landstrasse 9, W. Germany Arch Pharmacol; 293(Suppl): R64 1976 The molecular aspects of oxirane formation from trichloroethylene and other chlorinated ethylenes are discussed. In vitro, chlorinated oxiranes formed from the corresponding ethylenes undergo rearrangement to carbonylic compounds. In all cases except trichloroethylene, the products of thermal rearrangement of synthesized oxiranes are identical to the in vivo metabolites identified in perfused rat liver preparations. With trichloroethylene, the photochemically synthesized oxirane undergoes rearrangement in vitro, in nonpolar solvents, to dichloroacetyl chloride; chloral is formed only under catalysis of Lewis acids. The in vivo metabolites are chloral hydrate and its reduction products trichloroethanol and trichloroacetic acid. These findings indicate an interaction of the oxirane with its biological environment. (I Refs) 197. STRUCTURAL PARAMETERS ASSOCIATED WITH CARCINOGENESIS (HALOGENATED OLEFINS, VINYL AND ALLYL ANALOGS AND EPOXIDES). (PP. 8-21) Fishbejn L Natl. Center Toxicological Res., Little Rock, AR, 72207 Structural Correlates of Carcinogenesis and Mutagenesis. A Guide to Testing Priorities? Proceedings of the Second Food and Drug Administration Office of Science Summer Symposium held in Annapolis, 31 August-2 September 1977. Office of Science. FDA Annapolis, MD HEW Publication No, (FDA)78-IQ46, 241 pp., 1978. A number of reportedly carcinogenic industrial comp ounds (including halogcnated olefins, vinyl and allyl analogs, and epoxides) were examined to determine if their structural, biological, and metabolic similarities could help predict their carcinogenicity. The chlorinated olefinic derivatives of major concern are vinyl chloride, vinylidene chloride, trichloroethylene, chloroprene, dichlorobutene. and perchloroethylene, all of which have been proved to be carcinogenic to humans and/or laboratory animals. Evidence suggests that all chlorinated ethylenes are metabolized to oxiranes (epoxides) as a first step. The oxiranes. which are strongly electrophilic, may react directly with cell nucleophiles or they may undergo intramolecular arrangements. No such structure-activity relationship has been demonstrated for the various vinyl and allyl analogs that are carcinogenic and/or mutagenic. In the case of the epoxides, the presence of a reactive functional group (as in epichlorohydrin) or a double bond (as in glycidaldehyde) near the epoxide appears to enhance carcinogenicity by allowing the chemical to act as a difunctional alkylating agent for macromoieucles. Greater reliance should be placed on mutagenicity testing and on definitive metabolic and pharmacokinetic studies to augment structure-activity predictions of carcinogenicity. (79 Refs) 198. CARCINOGENIC POTENTIAL OF CHLORINATED ETHYLENES. TENTATIVE MOLECULAR RULES. )PP. 171-175) Henschler D, Bonse G, Greim H Institut fur Toxikologie und Pharmakologie, Universitat Wurzburg, D-8700, Wurzburg, Germany Environmental Pollution and Carcinogenic Risks. Lyon, International Agency for Research on Cancer, IARC Scientific Publications No 13, INSERM Symposia Series, Vol. 52, 1976. The mechanisms of bioactivation and the mutagenic activity of the chlorinated ethylenes were studied with a short term in vitro system using metabolic activating liver microsomal enzymes. Chlorinated ethylenes were activated in mammalian metabolism to oxiranes. Asymmetric chlorine substitution of the ethylenes and oxiranes rendered the molecules unstable and mutagenic. Vinyl chloride was the most active; trichloroethylene and vinyiidene chloride were far less active but still significantly so. Chlorinated ethylenes metabolized via symmetric oxiranes (tetrachloroethylene, cisand trans-l.2-dichloroethylene) were relatively stable and were not mutagenic. It was concluded that mutagenic activity and possible carcinogenic potential were related directly to oxirane stability. (13 Refs) 199. STRUCTURAL CORRELATIONS OF CARCINOGENIC AND MUTAGENIC ALKYL HALIDES. (PP. 163-171) Simmon VF SRI International, Menlo Park, CA, 94025 Structural Correlates of Carcinogenesis and Mutagenesis. A Guide to Testing Priorities? Proceedings of the Second Food and Drug Administration Office of Science Summer Symposium held in Annapolis, 31 August-2 September 1977. Office of Science, FDA Annapolis, MD HEW Publication No. (FDA)78-I046, 241 pp,, 1978. The Ames Salmonella typhimurium microsome assay, using strain TAI00, was used to determine the mutagenicity of 23 alkyl halides, 10 of which were unknown carcinogens. Twenty-one of these halides were mutagenic with and without S-9 liver microsome mix. The mutagenic compounds (c denotes proven carcinogen) included methyl bromide, methyl chloride, methyl iodide (c), methylene chloride (c), bromochloromethane, methylene bromide, bromoform (c), dibromochloromethane, bromodichloromethane, vinyl chloride (c), vinylidene chloride (c), 1,1,2-trichloroethylene (c), bis(2-ch!oroethyl) ether (c), bis(2-chloroisopropyl) ether, 1chloropropene, 3-chloropropene, epichlorohydrin (c), hexachlorobutadiene, 3-bromopropionic acid, 3iodopropionic acid, and 3-chloropropionic acid. Two known carcinogens, carbon tetrachloride and chloroform, were not mutagenic in the presence or absence of the S-9 mix. It is likely than that an insufficient amount of the mutagenic form was produced or that this form was so unstable it was unavailable to interact with the bacterial DNA. In general, the mutagenicity correlated with chemical reactivity. Of interest was the mutagenicity of methylene chloride, a widely used industrial chemical with broad environmental exposure (via paint removers and aerosol spray cans). (17 Refs) 200. MUTAGENICITY OF DICHLORVOS AND OTHER STRUCTURALLY RELATED PESTICIDES IN SALMONELLA AND STREPTOMYCES. Carere A. Ortali VA, Cardamone G, Morpurgo G Istituto Superiore di Sanita, Universita di Roma, Rome, Italy Chem Biol Interact; 22<2/3):297-308 1978 The following pesticides: azinphosmethyl, diallate, dichlorvos. EPTC (S-ethyldipropyl thiol + carbamate), 44 UCC 106367 fenchlorphos, mevinphos, monocrotophos, noruron, parathionmethyl, triallate, trichlorphon and vegadex were tested for the ability to induce his+ revertants in four histidine-requiring strains of Salmonella typhimurium, TAI 535 (missense), TAI 536, TAI 537 and TAI 538 (frame-shift), and resistance to low levels of streptomycin in Streptomyces coelicolor. Dichlorvos, which is a phosphoric ester with a dichlorovinyl group as side chain, and trichlorphon, which is known for its spontaneous conversion in dichlorvos, are both mutagenic in Salmonella (strain TAI 535) and Streptomyces. Five organophosphorus pesticides similar to dichlorvos but devoid of the vinyl group are not mutagenic. Three carbamates, diallate, triallate and vegadex, which contain a chloroallyl group similar to the vinyl group of dichlorvos are mutagenic in Streptomyces; triallate and vegadex are powerful mutagens also in Salmonella (strain TAI 535); two other carbamates devoid of the chlorinated group are not mutagenic. The results suggest that the presence of a vinyl chloride or allyl chloride group in the molecule of these pesticides is responsible for the ability to induce point mutations in Salmonella and Streptomyces. (Author abstract) (27 Refs) V. EPIDEMIOLOGICAL ASSOCIATIONS OF VINYL CHLORIDE AND RELATED COMPOUNDS WITH HUMAN CANCERS The reader may also find the following abstracts of interest: 236, 256. 290, 300 201. ANGIOSARCOMA OF THE LIVER IN VINYL CHLORIDE/POLYVINYL CHLORIDE WORKERS: 1977 UPDATE OF THE NIOSH REGISTER. Spirtas R, Kaminski R Illness Effects Section, SB/DSHEFS/NIOSH, Robert A. Taft Labs., 4676 Columbia Parkway, Cincinnati, OH, 45226 J Occup Med; 20(6):427-429 1978 Data on 64 cases of angiosarcoma among vinyl chloride (VC) polymerization workers reported as of October 1977 are summarized. The material comprises 23 US and 41 foreign cases from 11 different countries. The age at diagnosis ranged from 37 to 71 yr, with a median of 49 yr. The latency period ranged from 9 to 38 yr (median of 21 yr), duration of exposure from 4 to 31 yr (median of 18 yr). The data suggest that the reported VC-induced angiosarcoma cases gradually increased over time. The 20-to 30-yr lag between the growth of the industry after World War II and the increase in the number of diagnosed angiosarcomas among VC/polyvinyl chloride (PVC) workers during the I970's roughly coincides with the median latency period of 21 yr. Recent studies have also found VC to be associated with excesses in brain cancer and neoplasms of the respiratory and lymphatic systems. (6 Refs) 202. MORTALITY EXPERIENCE OF WORKERS IN A VINYL CHLORIDE MONOMER PRODUCTION PLANT. Buffler PA, Wood S, Eiflcr C, Suarez L, Kilian DJ Univ. Texas Sch. Public Health, P. 0. Box 20186, Houston, TX, 77025 J Occup Med; 21(3): 195-203 1979 A mortality follow-up study was conducted of 464 white men employed in a vinyl chloride monomer (VCM) pro duction plant for at least two consecutive mo between 1948 and 1975. Eight of the 28 deaths in this group were due to malignant neoplasms, 4 from lung cancer. No angiosarcomas or other liver tumors were observed. The eight persons who died of cancer were initially exposed to VCM prior to 1963, and the four with lung cancer, prior to 1958. Six of the 28 deaths, including 2/8 cancer deaths, occurred among a sub group of 165 workers exposed to 1,4-dioxane. The total number of observed cancer deaths was not significantly different than that expected, but a significant excess was noted for malignant neoplasms of the respiratory system. The effects of smoking, duration of exposure to VCM, and level of exposure and the combined effect of duration and level of exposure were analyzed separately. A 5 yr latency requirement was maintained for all analyses except for the smoking analysis. Using a minimum latency period of 5 yr from the date of initial exposure to VCM, the excess of respiratory cancer was moderate but not significant for the 314 employees satisfying this criterion. Both a longer duration and a higher level of exposure during the first 5 yr were associated with a signficant excess of respiratory cancer. However when duration and level of exposure were combined, t he results were not significant. In spite of the discrepancy in the results of dose-response analyses, the results suggest that a relationship exists between exposure to VCM and respiratory cancer. (27 Refs) 203. MEDICAL SURVEILLANCE SYSTEM FOR NEOPLASTIC AND NON-NEOPLASTIC OCCUPATIONAL INJURIES DUE TO INDUSTRIAL CHEMICALS (MEETING ABSTRACT). Tamburro CH, Creech JL, Greenberg RA, Makk L, Whelan JG Cancer Center, Div, Digestive Diseases and Nutrition, Univ. Louisville, Sch. Medicine, Louisville, KY Clin Res; 27(2):285A 1979 Following the discovery of a rare liver cancer (angiosarcoma) among vinyl chloride polymerization workers in 1974, a prospective medical surveillance program was designed for the early subclinical detection of occupational injuries, including neoplasia. This prototype program involved the active cooperation of a local hospital, a regional university cancer center, the plant workers, labor leaders, management and regulatory agencies. In the first 4 yr of operation, this pro gram identified an increased incidence of hepatic angiosarcoma among vinyl chloride workers, as well as an increased occurrence of such non-neoplastic, and possibly premalignant, lesions as peliosis hepatis, portal fibrosis, portal hypertension, splenomegaly and mid-zonal pleural fibrous thickening of lung. A systematic multi-disciplinary evaluation system was developed to determine if the diseases and disorders detected could be related to occupational chemical exposure. This program identified the job-related nature of hepatic angiosarcoma and its causative agent from among 22 different chemicals via individual chemical exposure histories based on a retrospective rank-order system for the various job classifications. This medical health surveillance system can be applied in any industry effectively without significant interference in workers' life or industrial function, at a cost effective level (av cost less than 5,000 dollars/yr/1000 workers provided that all participating parties actively cooperate, (no Refs) 204. PRIMARY LIVER CANCER DETECTION IN VINYL CHLORIDE WORKERS (MEETING ABSTRACT). (PP. 425-426) Whelan JG, Creech J, Tamburro CH Digest Dis and Nutri Sect and Cancer Ctr, Univ Louisville, Sch Med, Louisville, KY 40201 Third International Symposium On Detection And Prevention Of Cancer, 1976. The recent discovery of hepatic angiosarcoma in vinyl chloride polymerization workers led to a medical screening program which utilized radioisotopic scanning as a primary 45 UCC 106368 screening procedure among asymptomatic workers. Nine hundred employees underwent medical examination, biochemical blood studies and "mTc hepatic scanning. Sixtysix oT these employees had hepatic angiographic studies and hepatic biopsy. Thirty-five were studied because of pathological abnormalities on liver scan and 30 because of biochemical abnormalities with normal scans. Thirty-one percent of those with abnormal liver scans had angiographic lesions. Five had angiosarcoma, 2 of whom had no biochemical abnormalities at the time their scan indicated a lesion present. Four had cirrhosis and 2 peliosis hepatis. Of the 31 employees with normal scan, only 7% (2) had angiographic lesions; both had peliosis hepatis. Within one year, one developed a positive liver scan associated with the progression of his peliosis hepatis and the development of hepatic arterialportal venous shunts. The majority of those with positive liver scans without angiographic lesions did have significant histological disease including portal fibrosis, granulomatosis and fatty metamorphosis, In contrast, only minor nonspecific histological abnormalities were found in those with normal scans and negative angiographic studies. These data illustrated the effectiveness of the liver scan as a primary screening procedure both by its early detection of hepatic tumors and its low incidence of false positivity (less than 3%) in this cohort of asymptomatic vinyl chloride polymerization workers. In ad dition, vascular abnormalities such as peliosis hepatis, possibly a pre-malignant lesion, may also be detected in the absence of biochemical abnormalities. (Author Abstract) 2(15. VINYL CHLORIDE - PART 1. Potter HR No affiliation given Food Cosmet Toxicol; 14(4):347-349 1976 Workers involved in the production of polyvinyl chloride (PVC) are exposed to differing levels of vinyl chloride monomer, the greatest exposure being to those involved in cleaning the polymerization autoclaves. These exposures can lead to acro-osteolysis and to angiosarcoma. Less severe exposure .can occur during the subsequent processing of PVC, and although clinical changes may not be manifest, pathological tests detect effects characteristic of clinically advanced cases. An association of PVC manufacture with hemangiosarcoma was first suspected as a result of several deaths in the industry from this otherwise rare type of liver tumor. Systematic tests carried out in an attempt to relate liver abnormality to VC exposure levels revealed two cases of angiosarcoma and nine cases of portal fibrosis in 274 PVC production workers, and two cases of portal fibrosis in 909 workers not associated with PVC production in a Louisville plant, (no refs) 206. ANGIOSARCOMA OF THE LIVER IN VINYL CHLORIDE/POLY VINYL CHLORIDE WORKERS. Lloyd JW Natl. Inst. Occup. Saf. Health, Rockville, Md. J Occup Med; l7(5):333-334 1975 Review of recent data on cases of liver angiosarcoma il lustrated a clustering within recent years. Although the high risk of the disease for polymerization workers had previously gone unnoticed, an exact estimate of the incidence of liver angiosarcoma among the workers could not yet be determined, pending exposed-to-risk figures. Approximately 5,600 men are currently employed in 36 U.S facilities, with 15 cases of liver angiosarcoma reported to date. Greater than 70% of the cases were initially employed in polymerization work more than 20 yr ago. setting an average latent period of 19 yr. The age at diagnosis of angiosarcoma among both vinyl chloride workers and polymerization workers was considerably lower than that of the general population, sug gesting the possibility of a common etiology. Preliminary findings from continuing epidemiologic investigations indicated that polymerization workers may also be at high risk for other malignant neoplasms, particularly of the brain. It remains to be determined whether exposure to lower levels of vinyl chloride also represents excessive risk for liver angiosarcoma or other malignancies. 207. EPIDEMIOLOGICAL STUDIES OF VINYL CHLORIDE HEALTH EFFECTS IN THE UNITED STATES (MEETING ABSTRACT). Falk H, Waxmeiler RJ Center for Disease Control, United States Public Health Service, Dept, Health, Education, and Welfare, Atlanta, GA 30333 Proc R Soc Med; 69(4):303-305 1976 The high incidence of hepatic angiosarcoma in the vinyl chloride industry in the United States has prompted a large number of surveys; the results of three of these are examined. A review of death certificates indicated that angiosarcoma comprised 35% of all hepatic sarcomas between 1966-1973; there was also a 2:1 maleTemale ratio, suggesting that occupational exposure was important in the etiology, A study of polyvinyl chloride workers and controls showed no significant differences between the groups in six liver function tests but these tests are not totally reliable for vinyl chlorideinduced disease. In the third study, 12 lung cancer cases were identified in vinyl chloride-exposed workers, but the correlation was not as close here as it was with hepatic angiosarcoma. 208. VINYL CHLORIDE EXPOSURE IN A CONTROL LED INDUSTRIAL ENVIRONMENT: A LONG-TERM MORTALITY EXPERIENCE IN 594 EMPLOYEES Ott MG, Langner RR, Holder BB Dow Chemical Co., Corporate Medical Dept., 2030 Dow Center, Midland, Mich. 48640 Arch Environ Health; 30(7):333-339 1975 The relation between tumor incidence and the highest levels of vinyl chloride exposure was examined. Measurements of the work environment began in 1950 by collecting breathing zone samples on silica gel and measuring chlorides by the VoIhard method to calculate vinyl chloride. More recently, a combination of gas chromatography and mass spectrometry has been used to identify airborne material. The study po pulation included production employees who worked between 1942 and I960 in areas of potential vinyl chloride exposure. Each job was classified according to time-weighted average (TWA) concentration of vinyl chloride for an eight-hr day. The high level concentration was defined as over 200 ppm vinyl chloride, and the low level was below 25 ppm. Observed deaths among vinyl chloride workers were 91% of the expected deaths based on the U.S white male population. Distribution of malignant neoplasms suggests a possible dose-response relationship, since nine of 13 malignancies were observed in the high exposure group. The Chi 2 comparison of the distribution of malignancy deaths between high and all other exposure groups was significant (P is less than 0 01). No adverse malignancy effects are demonstrated in the lower exposure categories. 209. MORTALITY EXPERIENCE OF A COHORT OF VINYL CHLORIDE-POLYVINYL CHLORIDE WORKERS. Nicholson WJ, Hammond EC, Seidman H, Selikoff IJ Mt. Sinai Sch. Med., City Univ. New York, N.Y. Ann NY Acad Sci; 246:225-230 1975 The medical histories and current health status were obtained from 255 of 257 Goodyear Tire and Rubber Company employees who had been exposed to polyvinyl chloride (PVC) and vinyl chloride (VC) for a minimum of five 46 UCC 106369 yr subsequent to 1946. Observation began ten yr after the onset of exposure. The individuals worked in PVC production majority, maintenance, shipping, and in the laboratory. These workers may often have been exposed to peak VC concentrations exceeding 1,000 ppm. Over 50% of the employees were under age 37 as they entered the cohort. Twenty-four individuals were deceased and three of these deaths were due to liver hemangiosarcoma. One glioblastoma, two lymphomas, and one liver cirrhosis with associated bleeding esophageal varices accounted for other deaths. All of these conditions appear to be associated with VC. The three hemangiosarcomas occurred in individuals exposed prior to 1951. Longer periods of observation are thus needed to study the full spectrum of VC-induced malignancies and their incidence among exposed workers. 210. MORTALITY STUDY OF WORKERS IN THE MANUFACTURE OF VINYL CHLORIDE AND ITS POLYMERS. Tabershaw 1R, Gaffey WR Tabershaw-Cooper Assoc,, Ins., Rockville, Md. J Occup Med; I6(8):509-5I8 1974 This study of 8384 men who had at least one yr of occupational exposure to vinyl chloride before December 31, 1972, demonstrates that cancers of the digestive system (primarily angiosarcoma), respiratory system, brain, and cancers of unknown site, as well as lymphomas, occurred more often than expected in those members of the study population with the greatest estimated exposure. The mortality from other cancers was lower than that of the general male population with the exception of cancer of the buccal cavity and pharynx. There was an excess of these cancers, which however was inversely related to estimated exposure. No explanation has been found for the latter finding. The other major findings of the study are; I) the overall mortality of the study population was approximately 75% of what would be expected in a comparable population of U. S males; 2) no cause of death showed a statistically significant excess over what would be expected in a comparable U. S male population; and 3) no deaths identified as angiosarcoma of the liver were found other than those previously identified. This is the first epidemiological study suggesting that vinyl chloride may also be associated with human cancer of multiple sites. 211. PROPORTIONAL MORTALITY AMONG VINYL* CHLORIDE WORKERS. Monson RR, Peters JM, Johnson MN Harvard Sch. Public Hlth, Boston, Mass. Lancet; 11(7877):397-398 1974 . In a proportional-mortality analysis of 161 deceased workers in two plants using vinyl chloride, a 50% excess of deaths due to all cancer was seen. Specific sites of cancer with the greatest excess included liver and biliary tract, lung, and brain. The excess in fatal cancer was seen mainly in men who died before age 60. Also, there was a trend in time in the ratio of observed to expected deaths: since 1970 the expectancy of deaths from cancer has more than doubled. 212. HEPATIC DISEASE AMONG WORKERS AT A VINYL CHLORIDE POLYMERIZATION PLANT. Falk H, Creech JL, Heath W, Johnson MN, Key MM Center Disease Control, Atlanta, Ga. JAMA; 230(l):59-63 1974 Eleven cases of hepatic disease, including seven cases of hepatic angiosarcoma, have been identified to date among men employed at one vinyl chloride polymerization plant in Louisville. The earliest diagnosis was made in April 1964. The two most recent cases, both angiosarcoma, were diagnosed in February 1974 as a result of systematic medical screening for liver abnormalities among workers at the plant. Ages at diagnosis haw; ranged from 36 to 58 >r for the sesen patient:, with angiosarcoma and from 28 to 56 yr for the four patterns with nonmalignant disease; durations of employment before diagnosis have ranged from 12 to 28 yr (average 18 0) and from 5 to 29 yr (average 20 6). All 11 persons had worked in close and continuous contact with various phases of the vinyl chloride polymerisation process. Review of pathologic material suggests the presence in both tumor and nontumor cases of portal fibrosis and atypical sinusoidal lining cells. A direct causal relationship between exposure to vinyl chloride monomer and pathologic findings is postulated. 213. TEN CASES OF ANGIOSARCOMA OF THE LIVER IN SHAWINIGAN, QUEBEC. Delorme F, Theriault G Dept. Pathology, Centre Hospitalier Regionale de la Maurice, Shawinigan, Quebec, Canada J Occup Med; 20(5):338-340 1978 Of the 10 cases of angiosarcoma of the liver diagnosed in Shawinigan, Quebec, since 1955, all occurred in men who worked in a vinyl chloride (VC) polymerizing plant. All 10 men were very light drinkers, and only 4 smoked one or more packs of cigarettes per day. The time between the first exposure of VC monomer and the diagnosis of angiosarcoma ranged from II to 29 yr, with an av of 20.5 yr. Exposure to VC monomer in the plant, although not documented, is believed to have been very high in the past. The benign liver lesions found in these workers included portal and peristnusoidal fibrosis. (15 Refs) 214. MORTALITY EXPERIENCE OF WORKERS EXPOSED TO VINYL CHLORIDE MONOMER IN THE MANUFACTURE OF POLYVINYL CHLORIDE IN GREAT BRITAIN. Fox AJ, Collier PF Office Population Censuses and Surveys, St. Catherines House, 10 Kingsway, London WC2B 6Jp, England Br J Ind Med; 34(I):I-I0 1977 The mortality incidence in 7,717 workers in the vinyl chloride industry in Great Britain was investigated. Approx 99% of these workers were traced; 12% had been exposed to constant high levels, but only 34 men had been exposed to constant high levels for greater than 20 yr because of the newness of the industry. The standard mortality ratio was below that expected. Four cases of liver cancer were found, one primary cancer (vs 0.71 expected), and three other liver cancers (vs 0.93 expected). Two were angiosarcomas, and the other two were carcinomas. The two workers with angiosarcoma died 8 and 21 yr after initiation of exposure to high concentrations. Other cancers vs their expected incidences were as follows: stomach 14 vs 15-33; lung, 46 vs 51.23; brain, 2 vs 3.66; and lymphatic and hematopoietic tissues, 9 vs 9.01. The observed death rate from all cancers was 115 compared to an expected 126.77; there is no evidence that cancer at sites other than the liver was associated with exposure to vinyl chloride. Conclusions drawn from this survey must be tempered by the reservation that the full impact of the problem may not yet be in evidence. (18 Refs) 215. ANGIOSARCOMA OF THE LIVER: AN EPIDEMIOLOGIC SURVEY. Brady J, Libcratore F, Harper P, Grecnwald P. Burnett W, Davies JN. Bishop M, Polan A, Vianna N Bureau Occupational Health and Chronic Disease Res., New York State Dept. Health, Empire Slate Plaza, Tower Building, Albany, NY 12237 J Natl Cancer Inst; 59(5): 1383-1385 1977 An epidemiology survey of angiosarcoma of the liver (ASL) revealed that the annual incidence rate (cases diagnosed 47 UCC 106370 1970 through 1975) among residents of New York Stale (excludmg New York City) was 0.25 per million. A case-control study of 26 patients indicated that direct exposure to arsenic, vinyl chloride (VC), and thorium dioxide was a significantly important factor in the etiology of ASL (p less than 0.02). Seven patients had documented exposure to these chemicals, but. 19 did not. The fact that 5/19 lived nearer to VC fabrication or polymerization plants than did their matched controls indicated that indirect modes of exposure, not specifically related to occupation, might be an important factor in the etiology of ASL. The possibility that other toxic substances (chlorinated hydrocarbons) may cause ASL was also mentioned. (17 Refs) 216. MORTALITY AMONG EMPLOYEES OF PVC FABRICATORS. Chia/ze L, Nichols WE, Wong O Div, Biostatistics and Epidemiology, Dept. Community Medicine and International Health, Georgetown Univ. Sch. Medicine, Washington, DC 20007 J Occup Med; l9(9):623-628 1977 A cross-sectional mortality study was conducted to identify any angiosarcoma deaths among 4,341 deaths that occurred during 1964-1973 among current or former employees of 17 companies engaged in polyvinyl chloride fabrication. No angiosarcoma deaths were found among the study population. In addition, distributions by cause of death among white male and female employees were compared with those for the entire population of the US, specific for color and sex and adjusted for age by Proportionate Mortality Ratios. Important excesses in total cancer were found among white employees. They appeared to be concentrated in cancers of the digestive system, particularly, cancers of the intestine. In ad dition. mortality from breast and urinary cancer among white female employees was high. Results suggest the need for continued investigation, (9 Refs) 217. MORTALITY AND CANCER MORBIDITY IN A GROUP OF SWEDISH VCM AND PVC PRODUCTION WORKERS. Byren D, Engholm G. Englund A. Westerholm P Kema Nord. 85013 Sundsvall, Sweden Environ Health Perspect: 17:167-170 1976 Mortality and cancer morbidity data are presented from studies of 750 workers employed in a Swedish vinyl chloride (VC)/ poly(vinyl chloride) plant since 1940. Observed/expected deaths from various causes were: 2/0.33 (brain cancer). 3/1.78 (lung cancer), 4/0.97 (cancer of liver/pancreas, including 2 angiosarcomas of the liver), 28/18.26 (total circulatory disease), 6/3.02 (cerebrovascular disease). 15/9.15 (myocardial infarction), and 5/ 1.48 (cerebral hemorrhage). Observed/expected morbidities were: 3/1.30 (lung cancer) and 2/0.48 (cancer of liver/pancreas). The excess of cancers of the iivcr/pancreas increased with latency time (time between first employment and end of follow-up). The possible etiology of the cardiovascular deaths is discussed. (7 Refs) 218. THE C ARC INOGENIC PROPERTIES OF VINYL CHLORIDE. de Engelse L, Emmelot P Netherlands Cancer Inst., Amsterdam Chem Weekbl: 70(28/29):5 1974 Recent studies on the carcinogenicity of vinyl chloride and related legislation are reviewed in the light of the significantly increased incidence of angiosarcoma of the liver in workers exposed to this compound in the air of polyvinyl chloride (PVC) plants. A total of 19 cases of angiosarcoma of the liver have thus far been detected in workers exposed to vinyl chloride. Angiosarcoma of the liver and other tumors developed in rats and mice exposed to 250 and 50 ppm concentrations of vinyl chloride for 4 hr/day, 5 days/wk, for several months. These findings have prompted a reduction of the maximum permissible concentration to 50 ppm, but a further reduction of this limit appears necessary. Extensive epidemiological surveys on the incidence of liver injury and liver tumors among active and retired workers in PVC manufacturing plants, and experiments on the carcinogenicity of vinyl chloride at concentrations lower than 50 ppm are being conducted. 219. CASE STUDY OF VINYL CHLORIDE AS A CARCINOGEN. Kobayashi Y Engineering Faculty, Yokohama Natl. Univ.. Yokohama, Japan J Jpn Soc Saf Eng; 15(2): 101-104 1976 Cases of hepatic angiosarcoma associated with vinyl chloride monomer (VCM) and various facets of the VCM problem are summarized. VCM production in Japan began in the I950's, and, in the United States, in the I930's. In Japan, since 1975, recommended VCM concentrations in the factory atmosphere range from 2 to 5 ppm, and provisions have been made for adequate ventilation. Governmental surveys of 10 representative Japanese factories from 1974 to 1975 showed that 97.3% of the work areas had atmospheric VCM concentrations of less than 3 ppm. Other factories had atmospheric VCM concentrations greater than 5-10 ppm. One case of hepatic angiosarcoma was recently reported among retired Japanese factory workers. According to the National Institute of Occupational Safety and Health (NIOSH, United States), 38 cases of hepatic angiosarcoma worldwide (United States, Canada, Europe) occurred since 1975 among workers exposed to VCM from 4 to 30 yr. 220. HAZARDS ASSOCIATED WITH EXPOSURE TO VINYL CHLORIDE AND POLYVINYL CHLORIDE MATERIALS. Diubankova EN, Bykhovskii AV F. F, Erisman Res. Inst. Hygiene. Moscow, USSR Gig Sanit; (l):69-74 1979 Current data on the hazards of vinyl chloride (VC), in general, and of the use of polyvinyl chloride packages, in particular, are reviewed. Seventeen cases of liver angiosarcoma have been reported in workers in the VC industry (compared with the total 45 cases reported in the world literature). In ad dition to liver angiosarcomas, the workers had an increased incidence of cancer of the respiratory, CNS, lymph, and hematopoietic systems. The long-term storage of alcohol in PVC bottles showed distinct organoleptic changes in the alc ohol that prompted a ban on the use of PVC containers for food products containing alcohol. (26 Refs) 221. TEN CASES OF ANGIOSARCOMA OF THE LIVER IN VINYL CHLORIDE WORKERS IN CANADA. Delorme F Centre Hospitaiier Regional de la Mauricie, C.P. 1130, Shawinigan-Sud, Quebec, Canada Ann Anat Pathol (Paris): 23(2):97-l04 1978 Ten cases of hepatic angiosarcoma were found among employees of a vinyl chloride plant in Shawinigan, Canada. All patients had been exposed to vinyl chloride vapors. The av age of the patients was SO yr (41-61 yr); the length of occupational exposure to vinyl chloride averaged 16 yr 10 mo (ranging from 5 yr. 8 mo to 26 yr); and the time lapse between first exposure and diagnosis was 20.5 yr (11-18 yr). Survival after diagnosis averaged 4.4 mo (1-8 mo). The risk of hepatic 48 UCC 106371 angiosarcoma appears to be highest among vat cleaners, pipe fitters, and polymerization workers. (16 Refs) 222. VINYL CHLORIDE: EPIDEMIOLOGICAL STUDIES AND PREVENTIVE MEASURES. Owen R London, England Adv Tumor Prev Detect Charact; 3:238-241 1976 Epidemiological studies are being made to assess the effects of occupational exposure to vinyl chloride in Great Britain. Cancer and mortality rates of 6,000-8,000 past and present workers in Eve polyvinyl chloride plants are being related to vinyl chloride exposure in terms of time and concentration. A retrospective study of cases of angiosarcoma of the liver is underway to evaluate the association between this disease and occupation. The medical and social histories of these cases are also being compiled. The United Kingdom has lowered its interim standards for vinyl chloride exposure to a ceiling value of 30 ppm and a time-weighted average of 10 ppm. 223. CANCER RISK AMONG WORKERS EXPOSED TO CHLOROPRENE. Lloyd JW Natl. Inst. Occupational Safety and Health, Public Health Service Center Occupational Safety and Health, Rockvil le, MD 20852 Ann NY Acad Sci; 271:91-93 1976 The limited information on the potential carcinogenicity of chloroprene is discussed, along with some recently initiated assessments of this carcinogenicity. Chloroprene is used primarily as a monomer for the manufacture of neoprene synthetic rubber (introduced by duPont in 1931). No excess of any disease related to vinyl chloride, including angiosarcoma of the liver, has been observed at duPont upon investigation of employees' records. Although a recent increase in deaths from lung cancer in this population was observed, further investigation revealed no relationship to work assignment. Two studies of approx 45,000 chloroprene workers in Russia are also discussed with respect to the development of lung and skin cancer in these individuals. (2 Refs) 224. LUNG CANCER INCIDENCE AMONG CHLORO PRENE HANDLING WORKERS. Khachatryan EA Fifth Clin. Hosp.. USSR Vopr Onkol; l8(6):85-86 1972 A study of 2934 chloroprene -handling workers (working over 25 years) revealed 34 patients (1.24%) with lung cancer. The average age of these patients was 44.5 years and the average length of their service was 8.7 years. In the first control group of 4780 workers including chauffeurs, furniture makers, firemen, benzene storehouse workers, and house painters, there were 22 patients with lung cancer (0.46%), In the second group of 6045 workers including electricians, carpenters, electric welders, tinsmiths, and furnace workers, 11 had lung cancer (0.8%). There were only four patients with lung cancer (0.0064%) among 6220 office workers. Sixteen patients with lung cancer were found among the factory workers studied (number not given). Thus, the workers in contact with chloro prene showed a high incidence of lung cancer as compared with other occupational groups. Of the total 87 patients with lung cancer, 66 (75.8%) had chronic bronchitis, 3 (3.4%) tuberculosis, I (1.1%) bronchial asthma, and 4 (3.4%) pneumonia. 225. MORTALITY OF WORKERS EXPOSED TO CHLOROPRENE. Pell S Medical Div.. L.l. du Pont de Nemours and Co,, Wilmington, DE. 19898 J Occup Med; 20(l):2l-29 1978 A study was undertaken to determine whether exposure to cnloroprene increases the risk of lung cancer. Data were obtained from historical prospective mortality studies of two cohorts, one consisting of 270 men first exposed between 1931 and 1948, and the other of 1,576 men first exposed between 1942 and 1957. The number of lung cancer deaths in each cohort (3 in the first and 16 in the second) were about the same as expected. Among maintenance mechanics in the second cohort, there were eight lung cancer cases (4 living and 4 dead). A crude morbidity analysis suggested that this group may have had an excess incidence of lung cancer. However, the absence of excess lung cancer mortality in other high exposure occupational groups indicates that chloroprene exposure does not increase the risk of lung cancer. (Author abstract) 226. CHLOROPRENE: OBSERVATIONS OF CARCINOGENESIS AND MUTAGENESIS. (PP. 205-217) Infante PF, Wagoner JK, Young RJ Industry-wide Studies Branch, Div. Surveillance, Hazard Evaluation and Field Studies, Natl. Inst. Occupational Safety and Health, Cincinnati, OH, 45202 Incidence of Cancer in Humans, Proceedings of the Cold Spring Harbor Conferences on Cell Proliferation. Vol. 4, Hiatt HH, Watson JD, Winsten JA, ed. Cold Spring Harbor. Cold Spring Harbor Laboratory, Origins of Human Cancer., 602 pp,, 1977. Observations of excessive lung and skin cancer associated with chloroprene production were reported in the Russian literature in 1972. However, it was not until 1974, when an epidemic of liver, brain, and lung cancer among vinyl chloride (VC) polymerization workers was identified, was attention in the US focused on the carcinogenic potential of chloroprene. Chloroprene is mutagenic in bacteria, causes recessive lethality in Drosophila, causes dominant lethality and chromosome aberrations in rat bone marrow cells, and has been associated with sterility in mice and rats. A significant excess of chromosome aberrations and a reduction in the numbers and motility of sperm have been reported in chloroprene workers, and a threefold excess of miscarriages has been reported among their wives. In the absence of adequate data, a final evaluation of the carcinogenicity of chloroprene cannot be made. However, given its other effects, the possible carcinogenicity of chloroprene may be only of academic interest from a public health point of view. The data for VC and chloroprene may serve as the basis for a more aggressive role by genetic toxicologists in the assay of industrial chemicals. (35 Refs) 227. A COHORT STUDY ON TRICHLOROETHYLENE EXPOSURE AND CANCER MORTALITY. Axelson O, Andersson K, Hogstedt C, Holmberg B, Molina G, De Verdier A Dept. Occupational Medicine, Regional Hosp., 701 85 Orebro, Sweden J Occup Med; 20(3): 194-196 1978 A cohort of 518 Swedish men exposed to trichloroethylene (TCE) was studied with respect to cancer mortality. The men were divided into high- and low-exposure categories as estimated by the av trichloroacetic acid level in the urine: those with levels greater than 100 mg/liter were in the high exposure group, and those with levels less than 100 mg/liter were in the low-exposure group. Requiring 10 yr of 49 UCC 106372 latency time after the date of first employment (assumed to correspond to the time of first exposure) resulted in 3,643 person-yr of observation. Subdividing the cohort with regard to 10 yr of latency time as to highland low-exposure categ ories resulted in 548 and 3.095 person-yr of observation, respectively. There was a close agreement between expected and observed number of cancer cases in both the high-/and low-exposure groups. No particular type of cancer was overrepresented, although there were two leukemias and two stomach cancers. Both the leukemia and stomach cancer cases were in the low-exposure category. It seems justified to consider TCE as a possible human carcinogen, but one that is not more potent than many other industrial compounds. There is probably no serious hazard at low exposures. (21 Refs) 228. MORTALITY AMONG LAUNDRY AND DRY CLEANING WORKERS (MEETING ABSTRACT). Blair A, Decoufle P, Grauman D Environmental Epidemiology Branch, NCI, Bethesad, MD, 20014 Am J Epidemiol; I08(3):238 1978 The possible carcinogenicity of tetrachloroethylene, the predominant dry cleaning fluid used today, led the authors to conduct a preliminary analysis of the causes of death for 315 laundry and dry cleaning workers, identified from union rec ords, in order to determine if this group experienced unusual site-specific mortality. A proportionate mortality ratio (PMR) was calculated using the observed number of deaths in the study group and an expected number derived from the general US population. Statistically significant excesses occurred for all malignant neoplasms (PMR = 1.32) and in particular cancers of the liver (ICD 156) (PMR = 6.08), lung (PMR 181). and leukemia among whites (PMR = 4.20). In addition, non-significant deviations occurred for cancers of the digestive system (PMR = 1.40), breast (PMR = 0.71) and cervix (PMR = 1,98) among females. The excess of cancer of the cervix and deficit of breast cancer among females is consistent with other data for women of a similar socioeconomic level. The increase in the relative frequency of liver cancer and leukemia is particularly intriguing in light of the report by the NCI Carcinogen Bioassay Program of an excess of hepatocellular carcinoma in mice exposed to tetrachloroethylene and a clinical report of five cases of chronic lymphocytic leukemia in a family operating a dry cleaning business. A cohort mortality study is underway to further elucidate the potential risks faced by this group, (no Refs) 229. CARCINOGENS IN DRINKING W ATER. Miller RW NCI. NIH, Bethesda, MD 20014 Pediatrics: 57(4):462-464 1976 Asbestos, the carcinogenic qualities of which when inhaled are considered the result of its physical properties, has been found in high concentrations in drinking water. On the other hand, animal studies have indicated that when asbestos is ingested, it is not carcinogenic. Human occupational exposure to asbestos, however, is associated with increased frequencies of gastrointestinal cancer, presumably from the swallowing of the compound. The high cancer mortality of whites in New Orleans is reported to be due to the presence of carcinogens in the water, despite the facts that the high cancer incidence is primarily in the lungs, women arc less affected than men, and different communities with the same water sup ply show different effects. Vinylehlorides. pesticides, and polycyclic hydrocarbons are considered the most harmful. ( hlorination leads to the formation of four carcinogens (chloroform, bromoform, hromodichloromethane, and dichloromethane). Chloroform is considered potentially the most dangerous, although no human correlations have been found. The fetus and child may be more susceptible than adults to these carcinogens. No basis for recommending changes in water supply exists. 230. HEALTH EFFECTS OF VINYL CHLORIDE. Tamburro CH Digestive Diseases and Nutrition Section, Sch, Medicine, Univ. Louisville, 511 South Floyd Street, Room 535, MDR Building, Louisville, KY, 40201 Tex Rep Biol Med; 37:126-144 1978 The health effects of vinyl chloride (VC) are reviewed, and a medical surveillance system for detecting and following its effects is described. An early finding in exposed human and animals is activation of morphological changes in the sinusoidal lining cells of the liver without evidence of hepatocellular toxicity. With progressive exposure to VC there is an increase in atypia of the sinusoidal lining cells, pro gressive worsening of the focal sinusoidal dilatation, and malignant transformation of the sinusoidal lining cells. Most data suggest that it is the endothelial lining cell that becomes malignant and forms the angiosarcoma. Peliosts hepatis is often seen in nontumorous portions of the liver; whether this is u precarcinogenic or preangiosarcomatous lesion in VC workers is under study. Preliminary epidemiological and histological data suggest that the frequency of lung cancer is increased among VC workers. Two cases are reported in which the duration and degree of exposure to VC were almost identical, yet one man developed primary hepatocellular carcinoma and the other angiosarcoma. The man in whom hepatocellular carcioma developed had a history of extensive alcohol consumption, which may have played a role in the inability of the hepatocytes to detoxify VC metabolites. A prospective medical surveillance system has been designed based on classification of industrial environments into three categories according to the level of possible carcinogens. (10 Refs) 231. ASSOCIATIONS OF CANCER MORTALITY WITH HALOMETHANES IN DRINKING WATER. Cantor KP, Hoover R, Mason TJ, McCabe LJ Health Effects Res. Lab., U.S. Environmental Protection Agency. Cincinnati, OH, 45268 J Natl Cancer Inst; 6l(4):979-985 1978 Age-standardized cancer mortality rates by site and sex for whiles in 76 US counties were compared with levels of chloroform, bromine-containing trihalomethane(s) (BTHM), and total trihalomethanefs) (THM) in the drinking water. The 76 counties are greater than 50% urban, and over half the po pulation is served by a sampled water supply. Using several demographic, socioeconomic, and industrial variables, a predicted mortality rate was computed and compared with the actual rate. The resulting residual mortality rate for each county was compared with the level of one of the three THM indicators by use of the bivariate correlation coefficient. Counties were grouped according to the overall percentage of the county served by the sampled water supply (low, intermediate, or high) and by geographic location. Bladder cancer residual mortality in both sexes showed a strong correlation with BTHM. This correlation persisted in spite of controls for social class, ethnic group, urban vs rural residence, presence of specific high-risk industries, and lung cancer rates (possible surrogate for levels of cigarette smoking). Brain cancer was also correlated in both sexes, but the associations were not as strong, and there was regional variation. Other sites that gave indications of an association were; CHCI3 levels and male kidney cancer, BTHM and female lung cancer, and THM levels and non-Hodgkin's lym phoma in men. Stomach cancer in women showed a negative correlation, (23 Refs) 50 UCC 106373 VI. CLINICAL AND BIOLOGICAL EFFECTS OF OCCUPATIONAL EXPOSURE TO VINYL CHLORIDE AND RELATED COMPOUNDS A. Morphological Changes in Humans Exposed to Vinyl Chloride and Related Compounds 232. MORPHOLOGICAL CHANGES OF THE LIVER AFTER CHRONIC VINYL CHLORIDE INTOXICATION. Muller R, Bechtelsheimer H, Gedigk P, Marsteller HJ, Lelbach WK Pathologisches Institut der Universitat, D-5300 BonnVenusberg, West Germany Leber Magen Darm; 5(5):204-208 1975 The liver histopaihology of 50 workers exposed to vinyl chloride was examined from biopsy material. Three of the probands presented with polycentric hemangioendothelial sarcoma extending along the ectatic sinusoids into the parenchyma. In border areas a transition between proliferating atypical sinusoid cells and tumor cells was ap parent, typical of the angiosarcomas reported after chronic arsenic and thorium dioxide exposure. Septal fibrosis of the portal fields and collagenization of sinusoid walls, chainlike proliferation of sinusoid endothelial cells with nuclear polym orphism, hepatocytic hyperplasia with nuclear polyploidy, and focal adaptation of the hepatocytic cytoplasm (with hy perplasia of the smooth endoplasmic reticulum) to hydropic swelling, fat vacuolization, and cell necrosis were signs of generalized hepatotoxicity. Hepatocytic hyperplasia was most pronounced in the areas of the reactive sinusoid endothelium and at tumor interfaces. 233. HISTOPATHOLOGY OF LIVER LESIONS ASSOCIATED WITH EXPOSURE TO VINYL CHLORIDE MONOMER (MEETING ABSTRACT). Weinbren K Royal Postgraduate Medical Sch., Du Cane Road, London WI2 OHS, England Proc R Soc Med; 69(4)-.299-303 1976 An outline of the cell characteristics of angiosarcoma of the liver and changes that have occurred, with and without tumor development, in patients exposed to vinyl chloride monomer are presented. The tumors appear as dark hem orrhagic nodules with distortion and fibrosis in the intervening tissue; frank necrosis is often present. There are three types of involvement; the sinusoidal pattern, the papillary type, and the cavernous type. The cells are usually associated with vessel walls and vascular spaces. Near the tumor and sometimes in the absence of tumors, perisinusoidal reticular fibers are prominent and fibrosis in the portal tracts is often reported. Subcapsufar fibrosis may also occur. The relevance of these nontumorous changes to the subsequent development of angiosarcoma is unknown at this time. 234. ULTRASTRUCTURE OF LIVER DAMAGE IN CHRONIC VINYL CHLORIDE INTOXICATION. Schattenberg PJ, Totovic V, Gedigk P, Marsteller HJ Pathologisches Institut dcr Universitat Bonn, Postfach 2120, D-5300 Bonn, Bundesrcpublik Deutschland Virchow Arch Pathol Anat Histol; 373(3):233-247 1977 Liver biopsies taken from 15 workers at a PVC-producing factory were examined by electron microscopy. The hepatocytes showed focal hydropic swelling, disseminated toxic steatosis, peculiar para-crystalline inclusions in enlarged mitochondria, focal cytoplasmic degradations, and occasional single cell necroses. These regressive changes were more prominent in cases with a shorter interval of non-exposure prior to the biopsy further, a local compensatory hyperplasia of the smooth endoplasmic reticulum was found. With increase of the non-exposure lime interval, a regression of the degree of steatosis us well as an age-independent excessive Itpofusctn deposition was seen in the hepatocytes. Apparently, these are sequelae of increased autophagia of lipids and increased lipid oxidation by the vinylchlonde. In the sinusoids, activation, enlargement and proliferation of Kupffer cells were noted. The tendency of these cells to proliferate is apparently caused by the cancerogentc stimulation by vmylchloride. The prominent hyperplasia of lipocytes is probably connected with the deposition of collagen and the peculiar perisinusoidal fibrosis. (28 Refs) (Author Abstract) 235. CLINICAL AND MORPHOLOGIC FEATURES OF HEPATIC ANGIOSARCOMA IN VINYL CHLORIDE WORKERS. Makk L. Delmore F, Creech JL, Ogden LL. Fadell bH, Songster CL, Clanton J, Johnson MN, Christopherson WM Department of Clinical and Anatomical Pathology, St. Anthony Hospital, Louisville, Ky 40204 Cancer Suppl; 37(1): 149-163 1976 Fifteen male workers exposed to vinyl chloride developed angiosarcoma of the liver. Thirteen died of disease and two are currently living for short periods after diagnosis. Their ages ranged from 36 to 58 years (average 47.5 years). Their exposure time ranged from 4 to 27.8 years (average 16.9 years). The most common presenting symptoms were fatigue, weight loss, and abdominal pain. Hepatomegaly followed by splenomegaly were the most common physical findings. Biochemical profiles yielded variable results and proved to be of little value in the detection or diagnosis. Of eight patients aulopsied, distant organ involvement was present in two cases, duodenal involvement in one, and direct extension of tumor to adjacent organs or tissues in four additional ones. The remainder, diagnosed by open liver biopsy, revealed no tumor extension. The gross features of the tumors were hemorrhagic necrosis, cystic degeneration, fibrosis, and apparent multicentricity. The histologic features were those of the typical angiosarcoma found in a variety of sites with a wide range of cellular differentiation. The histologic diagnosis was often impaired by the extensive tumor necrosis. Elsewhere in the liver subcapsular fibrosis, a distinct type of portal fibrosis, and endothelial cell hyperplasia with or without sinusoidal dilatation were noted. The reduction of industrial chemical exposure has already been achieved and will hopefully eliminate this chemically related tumor in the future. There is, however, a significant group of previously exposed workers who will require careful monitoring to delect functional abnormalities of the liver and possible early neoplastic changes. 236, DEVELOPMENT OF HEPATIC ANGIOSARCOMA IN MAN INDUCED BY VINYL CHLORIDE. THOROTRAST. AND ARSENIC, COMPARISON WITH CASES OF UNKNOWN ETIOLOGY. Popper H, Thomas LB, Telles NC, Falk H, Selikoff IJ Stratton Lab. Liver Disease, Mount Sinai Sch. Medicine, City Univ. New York, New York. NY Am J Pathol; 92(2):349-376 1978 Human hepatic angiosarcomas that occurred following exposure to vinyl chloride, Thorotrast. or arsenic (medicinal and industrial) and cases, including those in children, of unknown etiology were studied to establish diagnostic criteria and evolutionary features. The uniform evolution suggests that there is also an environmental factor in the cases of unknown etiology. The precursor stage is characterized by areas of combined hyperplasia of hepatocytes and a variety of sinusoidal and perisinusoidal cells associated with an excess of 5) UCC 106374 rettcu 1 in Lind with sinusoidal dilatation. Silver stainings indicated reticulum formation by the perisinusoidal cells, presumably the lipocytes. The hepatocytic proliferation sug gested a hepatocarctnogenic, but usually not fully expressed, potential The mixed hyperplasia of the various sinusoidal cells proceeded to an overgrowth of angiosarcoma cells, presumably derived from endothelial cells In early stages they were usually in contact with hepatocyte (intralobular growth). A trabecular arrangement resulted from loosening of the lo bular plate arrangement by dilatation of sinusoids, leading to primary peiiosis. With disappearance of the hepatocytes, various growth patterns developed, terminating in nodular, solid angiosarcoma composed of either spindle-shaped or polyhedral cells that underwent necrosis or hemorrhage (secondary peiiosis). The interaction between hepatocytes and sinusoidal cells requires elucidation. (110 Refs) 237. HEPATIC ANGIOSARCOMA IN WORKERS FOL LOWING CHRONIC EXPOSURE TO VINYL CHLORIDE. MORPHOLOGICAL DESCRIPTION OF THE LESIONS. Delorme F, Makk L Centre hospitalier regional de la Mauricie, C.P, 1130, Shawinigan-Sud, Quebec, Canada Union Med Can; I04( 12); 1836-1844 1975 The histological characteristics of liver angiosarcomas (2 mctastases) in 18 men (36 to 58 yr; mean age 46.7) who had prolonged exposure to vinyl chloride (4 yr to 27 yr 10 mo; mean lime 17 yr 4 mo) are presented. Various benign lesions were noted not only in the angiosarcoma cases but also in noncancerous cases who also had prolonged exposure. Many of the perisinusoidal cells of the angiosarcomas resembled, with their elongated nuclei, the small mesynchymatous cells of the benign tumors. The angiosarcomas had different forms: sinusoidal, sarcomatous, nodulopapillary, and cavernous. These cancers are generally preceded by the appearance of ten benign lesions induced by the vinyl chloride. They appear simultaneously in many places and apparently are multifocal. The numerous nodules are not intraparenchymal metastases from a primary tumor but are themselves primary tumors. 238. ENZYME HISTOC HEMICAL, HISTOMETRICAL AND ULTRASTRUCTURAL STUDIES OF SPLEENS IN VINYLCH LORI DE-DISEASE. Heusermann U, Stutte HJ Klinikum der Christian-Albrechts-Universitat Kiel, Abreilung Allgemeine Pathologic und Pathologische Anatomic, Hospitalstr. 42, D-2300 Kiel, W. Germany Virchows Arch Pathol Anat; 375(4);303-317 1977 By means of histomctric, enzyme histochemical. and electron microscopic investigations it was demonstrated that the pathological changes in the spleen in vinylchloride-disease are primary. Fibroblastic cells are the only specific splenic cells involved. Fiber-associated reticulum cells of the red pulp and fibroblastic reticulum cells in white pulp are stimulated to pro duce excessive amounts of the extracellular elements of connective tissue, especially collagen fibrils. The newly formed connective tissue causes obliteration of extracellular blood channels in the red pulp and thus a reduction in the number of pulp-cord macrophages, and scarring of the periarterial lym phatic sheaths. The results of this fibrosing process are characteristic quantitative changes in the splenic histologic structures. These changes are different from those structural alterations occurring in spleens following exirasplenic hemodynamic changes, such as thrombosis of the splenic veins or cirrhosis of the liver. (Author abstract) (16 Refs) 239. THE CONDITION OF THE SPLEEN DURING VINYL CHLORIDE DISEASE (MEETING ABSTRACT). Stutte HJ, Heusermann U Kiel, W. Germany Zentralbl Allg Pathol: 122(3):284 1978 Contact with vinyl chloride during the production of various synthetic substances is unavoidable and may lead to severe organ alterations, the most frequently observed being splenomegaly with clinical signs of portal hypertension. Morphological examination indicates that the spleen is the target of fibrotic lesions with characteristic types, location, and dissemination. These parameters allow for successful histological confirmation of a clinically occult splenomegaly und the differentiation of a portal occluded spleen in case of hepatic cirrhosis. The most significant differential diagnostic criteria are presented in tabular form. With respect to these criteria, it is thought that the splenic alterations observed during vinyl chloride disease were not caused by portal hy pertension due to vinyl chloride induced hepatic alterations, rather by a direct fibrotic process in the red and white pulpa and connective tissue of the spleen induced by vinyl chloride or its metabolites, (no Refs) 240. CAPILLARY ABNORMALITIES IN POLYVINYL CHLORIDE PRODUCTION WORKERS. EXAMINATION BY IN VIVO MICROSCOPY. Maricq HR, Johnson MN, Whetstone CL, LeRoy EC Div, Rheumatology Immunology, Dept. Medicine, Medical Univ, South Carolina, 80 Barre St., Charleston, SC 28401 JAMA; 236(12):1368-1371 1976 To determine whether symptomatic vinyl chloride (VC) workers have finger-skin-capillary abnormalities, whether the abnormalities are similar or different from those in patients with idiopathic scleroderma and Raynaud syndrome, whether microvascular abnormalities are related to the type of VCassociated abnormalities (especially liver), whether microvascular abnormalities are related to the nature and duration of VC exposure, and whether microvascular abnormalities are found in workers not exposed to VC, the hands of 152 VC-exposed workers were examined by widefield capillary microscopy. Examination revealed scattered scleroderma-like microvascular abnormalities in 21 workers and isolated capillary abnormalities in another 27, as compared with only three isolated abnormalities in 50 manual workers not exposed to VC. Thirteen of 17 VC workers with objective evidence of VC-associated abnormalities (angiosarcoma or fibrosis of liver, acro-osteolysis, or scleroderma-like skin lesions) also had microvascular abnormalities. Since microvascular changes occur more frequently than overt VC-associated pathologic conditions, they may represent an early manifestation of the VC effect. Thus, capillary microscopy may become a useful massscreening procedure in the early detection and prevention of VC-associated disease. 241. PRECURSOR LESIONS IN EXPOSED PO PULATIONS AS INDICATORS OF OCCUPATIONAL CANCER RISK. Maltoni C Inst. Oncology and Tumour Centre, Bologna, Italy Ann NY Acad Sci; 271:444-447 1976 Occupational cancer risk was monitored on two separate occasions by cytological examinations. The first involved three groups of workers who were known or suspected of being at risk for developing pulmonary cancer chromium industry workers, vinyl chloride-polyvinyl chloride (VC-PVC) industry workers, and workers at different chemical factories not directly involved in the production or use of known carcinogens. The results of sputum cytology showed that the 52 UCC 106375 incidence of abnormal cases was higher than expected among chromium and VC-PVC workers, even when compared to po pulations of heavy smokers. The second occasion was the cytologic investigation of urinary sediments in a group of Italian dye stuff factory workers exposed to aromatic amines and, therefore, at risk for urinary tract tumors. A high incidence of 1% Tor Class III cytology and over was obtained. These results indicate both the value and need for cytologic examinations as preventive measures that can be taken to overcome occupational risks of carcinogenesis, (no Refs) translocations, and deletions. Only \n the proband group showing symptoms of the VC illness. Inclusive as well as exc lusive gaps, could a significant increase in the rate of aberrations be seen, in comparison to the control group. At the same time tests were made on the bone marrow of Chinese hamsters. The animals had been previously ireated with 2,500 and 5,000 ppm VC respectively taken in by inhalation and then with doses of 600 mg/kg body wt and 300 mg/kg respectivelygiven by ip injection. Both tests showed that the frequency of structural anomalies inclusive and exclusive gaps in the bone marrow cells was significantly higher in comparison to the control group, (no Refs) B. Cytogenetic Changes in Humans Exposed to Vinyl Chloride and Related Compounds 242. INDUSTRIAL MONITORING: A CYTOGENETIC APPROACH. Kilian DJ, Picciano DJ, Jacobson CB Dept. Industrial Medicine and Biomedical Res., Dow Chemical U.S.A., Texas Div,, Freeport, TX 77541 Ann NY Acad Sci; 269.4-11 1975 The authors describe the use of cytogenetic monitoring to detect changes induced by occupational exposures to chemicals, particularly to vinyl chloride. During a 10-yr period, 43,044 shorthand and conventional karyotypes of 1,689 production workers were studied by chromosome analysis correlated with a standardized medical history. Blood samples were drawn from each individual, slides were prepared, and a metaphase spread was located. The selected cells were photographed, the metaphase spread photographs were evaluated, and anomalous chromosomes were classified and tabulated according to the type of structural aberration. Chromosomes were karyotyped to permit detection of aberrations such as small deletions, translocations, and pericentric inversions. Preliminary data from the cytogenetic analysis of blood samples from 121 vinyl chloride workers were compared' with preemployment examination records from 75 individuals who served as controls. The following three differences were seen: vinyl chloride workers showed less chromatid breakage, a greater frequency of dicentric chromosomes, and a lower percentage of abnormal cells than the preemployment comparison group. It is concluded that chromosome analysis is the procedure of choice for objective observation and measurement of possible insults to human genetic material and that the techniques described are the best for the detection of genetic injury at a stage when corrective action can be taken. (8 refs) 243. EXTERNAL CHROMOSOME STUDIES UNDERTAKEN ON PERSONS AND ANIMALS WITH VC ILLNESS (MEETING ABSTRACT). Fleig I, Thiess AM Occupational Medicine and Health Protection, BASF, Actiengesellschaft, 6700 Ludwigshafen, W. Germany Mutat Res; 53(2): 187 1978 A chromosome analysis was undertaken on lymphocyte cultures taken from 30 workers from various polyvinyl chloride (PVC) factories throughout W. Germany, and of 10 control persons who had been matched according to age. The lymphocyte cultures had been incubated for 72 hr. The exposed workers were divided into two groups: (a) 10 persons with no symptoms of VC illness and (b) 20 persons with VC il lness symptoms such as acro-osteolysis, Raynaud syndrome, thrombocytopenia, liver function disturbances, etc. One hundred cells per individuals were scored for gaps, breaks, fragments, dicentrics, rings, chromatid and chromosome 244. MUTAGENICITY OF VINYL CHLORIDE. EXTERNAL CHROMOSOME STUDIES ON PERSONS WITH AND WITHOUT VC ILLNESS, AND ON VC EXPOSED ANIMALS. Fleig I, Thiess AM Dept. Occupational Medicine and Health Protection, BASF Aktiengesellschaft, 6700 Ludwigshafen, W. Germany J Occup Med; 20(8):557-561 1978 Chromosome analysis was performed on lymphocyte cultures from 6 workers with an estimated degree of exposure to vinyl chloride (VC), 4 workers whose degree of exposure was monitored, 20 workers showing symptoms of VC illness after unknown degrees of exposure, and I patient with VCinduced angiosarcoma. Bone marrow cells from Chinese hamsters exposed to VC by inhalation (2,500 or 5,000 ppm for 4 hr/day x 5) or ip injection (300 or 600 mg/kg/day x 5) were also analyzed. The frequency of chromosome aberrations was not increased relative to controls in the exposed workers with no signs of VC illness. The frequency of aberrant metaphases in the workers with VC illness was 5.2% excluding gaps and 11.2% including gaps, these values were significantly higher than the control values of 2.1% and 5.5%, respectively. The rate of aberrant metaphases in the angiosarcoma patient was 7.3% excluding gaps and isogaps and 16.6% including gaps and isogaps. The frequency of structural abnormalities in the VCtreated hamster cells was also significantly higher than that in the control cells, inclusive and exclusive of gaps. (15 Refs) 245. CYTOGENETIC INVESTIGATIONS ON LYM PHOCYTES FROM WORKERS EXPOSED TO VINYL CHLORIDE. Leonard A, Decat G. Leonard ED, Lefevre MJ, Decuyper LJ, Nicaise C Lab. Genetics, Dept. Radiology, C.E.N.-S.C.K. B 2400 Mol, Belgium J Toxicol Environ Health; 2(5):l 135-1141 1977 Eleven male workers from the polymerization department of a VC factory, seven people employed in the laboratory of another VC plant, and ten controls from outside the factory environment were examined for the presence of chromosome aberrations in blood lymphocytes and these cytologicul findings were viewed in light of the occupational and medical histories of the subjects. Most of the workers from the polymerization department had chromosome anomalies such as fragments, rings, translocations, and dicentrics. However, since these workers received frequent radiographs of the hands, feet, vertebral column and digestive tract, it is impossible to determine whether these chromosome anomalies result from vinyl chloride exposure or from diagnostic exposure to ionizing radiations. It is concluded that the risk of significant increase in chromosome aberrations due to occupational exposure to VC seems small and that present working conditions in VC plants are within acceptable safety limits. (17 Refs) UCC 106376 53 246. CYTOGENETIC INVESTIGATIONS ON LYM PHOCYTES FROM WORKERS EXPOSED TO VINYL CHLORIDE (MEETING ABSTRACT). Leonard A, Decat G, Leonard ED Mammalian Genetics Lab., C.E.N-S.C.K., B-2400 Mol. Belgium Mutat Res; 53(2):2I9 1978 Male workers from the polymerization department of a vitn I chloride (VC) factory, people employed in the laboratory of another VC plant and controls from outside the factory environment were examined for the presence of chromosome aberrations in blood lymphocytes. The incidence of chromatid aberrations (gaps and breaks) and of chromosome gaps was not significantly increased after VC exposure, but more severe chromosome anomalies such as chromosome fragments, rings and dicentrics were observed in most of the workers from the polymerization department. The medical history of the workers shows that most people from the polymerization department received several radiographies, The conclusion that the chromosome fragments and chromosome dicentics result from VC exposure remains, therefore, dubious. It is much more probable that these anomalies originate from the diagnostic exposure to ionizing radiation, (no Refs) 247. CHROMOSOMAL ANALYSES IN VINYL CHLORIDE-EXPOSED WORKERS. Purchase IF, Richardson CR, Anderson D, Paddle GM, Adams WG Central Toxicology Lab.. Central Medical Group and Mond Div,. Imperial Chemical Industries Ltd., Alderley Park, Macclesfield, Cheshire. England Mutat Res: 57(3);325-334 1978 The chromosomal morphology of cultured peripheral lymphocytes from 81 men 57 employed in plants manufacturing vinyl chloride (VC) or polyvinyl chloride (PVC). 19 on-site controls, and 5 off-site controls) was studied. There was a significant increase in chromosomal abnormalities among the VC/PVC-exposed workers compared with the controls. Total B cells (those with a chromatid break or gap or a chromosome gap), total Cu cells (those with larger unstable chromosomal abnormalities), and total Cs cells (those with larger unstable chromosomal abnormalities) showed the greater increases in autoclave operators, with smaller increases in the other five job categories. The increase in chromosomal aberrations was correlated with length of exposure and with a history of exposure to excursion levels of VC during the year prior to sampling (1973-1974), There were no correlations between results of- liver function tests and chromosome aberrations. Quantity of tobacco smoked 18 mo after blood sampling was correlated with total C cells, as was smoker vs nonsmoker status at 18 mo postsampling. It was not possible to determine w hether smoking history, length of employment, or exposure to excursion levels of VC was the most important variable m determining C cell abnormalities. (15 Refs) 248. C HROMOSOMAL DAMAGE IN MEN OCCUPATION ALLY EXPOSED TO VINYL CHLORIDE MONOMER AND OTHER CHEMICALS. Heath CW, Dumont CR. Gamble J. Waxweiler RJ Cancer and Birth Defects Div., Bureau Epidemiology, Center Disease Control, Public Health Service, U.S. Dept. Health. Education and Welfare, 1600 Clifton Road, N E., Atlanta, GA 30333 Environ Res; l4(l):68-72 1977 C'vtogcnetic analyses were conducted on men employed at a rubber and plastics plant who were exposed to vinyl chloride monomer (VCM). Frequencies of chromosomal damage were measured in the peripheral blood lymphocytes of 35 men employed for greater than or equal to 10 yr: 14 in polyvinyl chloride (PVC) polymerization (htgh-exposure group), 4 in PVC processing (low exposure group), and 17 in rubber tire manufacture (negligible exposure group). In addition, four nonindustrtal employees who had not been exposed to lab oratory chemicals were included (controls). Breakage levels were significantly higher in all three test groups than in the controls. Chromatid gaps comprised the majority (86%) of aberrations seen. However, overall breakage levels were similar in the three industry groups, which indicates that VCM was not the sole cause of the damage. (9 Refs) 249. VINYL CHLORIDE CYTOGENETICS. Picciano DJ, Flake RE, Gay PC, Kilian DJ Occupational Health and Medical Res., Dow Chemical, Freeport, TX 77541 J Occup Med: l9(8):527-530 1977 This report presents cytogenetic findings from a group of 209 workers employed for up to 28 yr in the manufacture of vinyl chloride monomer at the Texas Division of Dow Chemical USA. Cytogenetic evaluation results from this group were compared to results found in examination of individuals being considered for employment. Statistical analyses were performed on a group basis for chromatid aberrations, chromosome aberrations and proportion of abnormal cells: no statistical difference of significance was found between the two groups. Comparison of these results with reported studies sug gests that the level of cytogenetic aberrations in vinyl chloride workers is probably related to the length and level of exposure, and that risk of adverse genetic effect can be avoided in controlled, minimal-exposure environments. (Author abstract) (31 Refs) 250. EFFECTS OF VINYL CHLORIDE IN MAN. A CYTOGENETIC FOLLOW-UP STUDY. Hansteen IL, Hillestad L, Thiis-Evensen E, Heldaas SS Lab. Genetics. Telemark Central Hosp.. Olavsgt. 26, 3900 Porsgrunn, Norway Mutat Res; 51 (2):271-278 1978 For comparison, a second cytogenetic study was made of 37/39 polyvinyl chloride workers 2-2.5 yr later, during which time the workers had minimal exposure to vinyl chloride monomer (VCM). The second study was performed with 32 matched controls selected from office employees at the same factory. Of the original 39 workers, 14 had been chosen at random, 13 because they had heavy exposure to VCM for years, and 12 because of abnormal clinical findings that later proved to be normal. Breaks, gaps, and stable rearrangements were scored as 100 metaphases/person from 48-hr lymphocyte cultures. In the first study, the mean number of chromosome breaks was significantly higher for the workers (3.41%) than for the controls (1.79%). No significant difference was found in the second study. In addition, there was no significant difference in mean number of breaks between the first control group and both groups of the second study. The results of the two studies provide evidence that VCM was the cause of the chromosome damage found in the first study. In the second study, the mean number of sister chromatid exchanges per cell was the same (7.6) for both workers and controls. Studies of bone marrow samples from four workers revealed that these samples had a higher mean number of chromosome breaks (4.2%) than normal bone marrow (literature values, 0.2%1.7%) or the corresponding lymphocyte cultures. (19 Refs) 54 UCC 106377 251. HIGH RATE OF CHROMOSOMAL ABERRATION IN PVC WORKERS. Szentesi I. Hornyak E. Ungvary G. Czeizel A. Bognar Z, Timar N Lab. Human Genetics, Natl. Inst. Hygiene, H-1966 Budapest, Gyali ut 2-6, Hungary Mutat Res; 37(2-3>:313-316 1976 Chromosome examinations were performed on 45 polyvinyl chloride (PVC) workers exposed to vinyl chloride for 0.5 to 12 yr. Two control groups were used. The first was composed of 44 industrial workers who were not exposed to PVC and were only indirectly exposed to other chemicals. The second control group was composed of 49 individuals who had no occupational exposure to chemicals. The rate of numerical chromosome aberrations did not differ significantly between PVC workers and either control group. The frequency of chromatid-type aberrations was higher m PVC workers than in either control group. Unstable chromosome-type aberrations were also significantly higher in PVC workers. The PVC workers who showed a significantly higher frequency of chromatid -type aberrations or unstable chromosome-type aberrations had been exposed to the compound for longer periods than other PVC workers. They are to undergo a clinical check-up to determine whether they can continue in their present occupations. (17 refs) 4 252. CHROMOSOME STUDIES OF TRICHLOROETHYLENE WORKERS. Konietzko H, Haberlandt W, Heilbronner H, Reill G, Weichardt H Institut fur Arbeitsmedizin der Universitat, Wilhelmstrabe 27, D-7400 Tubingen, W. Germany Arch Toxicol (Berl); 40(3):201-206 1978 On the basis of lymphocyte cultures, chromosome studies were carried out on 28 employees involved in the purification of trichloroethylene (TCE) who were exposed from I to 21 yr to various concentrations of TCE. Nine of these subjects showed pathological percentages of hypodiploid cells, but otherwise they had normal karyotypes; one subject had the karyotype 47,XY, + mar (small metacentric extrachromosome). For these nine employees, it was found that the significantly higher chromosome aberration rate correlated with TCE concentration level but not with duration of exposure. (9 Refs) 253. HEALTH DAMAGE BY TRICHLOROETHYLENE: AN EPIDEMIOLOGICAL AND CLINICALEXPERIMENTAL STUDY. Konietzko H Institut fur Arbeits-und Sozialmedizin, Universitat Tubingen, Wilhelmstrasse 27, 7400 Tubingen, W. Germany Fortschr Med; 97( 14):671-674 1979 Trichloroethylene (TCE) levels were measured at 10 industrial sites. The 6-hr av were mainly between 52-94 ppm. The max allowable level (MAL) is 50 ppm. Chromosome aberrations increased in workers as the level of exposure to TCE increased. This finding indicates that the TCE levels observed present a carcinogenic risk and that lowering the MAL to 20 ppm should be considered, (no Refs) C. Teratogenicity of Vinyl Chloride and Related Compounds in Humans 254. SUR\ EILLANCE OF SPUN I'ANEOl S A BOR HONS. POWER IN ENVIRONMENTAL MONITORING. Kline J, Stein Z, Strobino B, Susser M, Warburton D Columbia Univ. Coll. Physicians and Surgeons, New Y ork, NY 10052 Am J Epidemiol; !06(5)'345-350 1977 The advantages of using spontaneous abortions rather than birth defects to monitor environmentally induced carcinomas are discussed. Theoretically, a change in the incidence of spontaneous abortions or of anomalies in spontaneous abortions should be detectable 6 mo or more before a change in the incidence of birth defects. Moreover, teratogens that cause many anomalies cannot be detected by studies of birth defects since the majority of anomalous conceptions lead to spontaneous abortions. Abortion specimens can also be preserved for future studies. The spontaneous abortion approach is illustrated by the potential teratogenic effects of paternal exposure to vinyl chloride (VC). VC may affect male germ cells by causing chromosome breaks, dominant or recessive lethal mutations, and/or chromosome abnormalities. Morphologic and karyotype analyses of a sample series of spontaneous abortions are discussed. (14 Refs) 255. ONCOGENIC AND MUTAGENIC RISKS IN COM MUNITIES WITH POLYVINYL CHLORIDE PRO DUCTION FACILITIES. Infante PF Ohio Dept, Health, Columbus, OH 43216 Ann NY Acad Sci ; 271:49-57 1976 An epidemiologic study was done on the distribution of congenital abnormalities in three cities with polyvinyl chloride (PVC) production facilities. The numbers of children with congenital malformations and rates per 1,000 live births were computed for each city over 4 yr. Mothers in the cities gave birth to an excess number of children with congenital malformations compared to that expected based on the state and county averages. Other county-city combinations matched by population size showed no significant differences in incidence of congenital malformations. Maternal age, race, and possible differences in hospital reporting were not considered accountable for the observed differences. Anomalies of the CNS were of the greatest concern. An excess number of CNS defects was observed among stillbirths and live births from the index cities, with one exception. The excess was largely from two of the cities. Deaths from CNS tumors in adult male residents in the same two cities were also significantly greater than expected. On a community basis, excess deaths from the other cancers were not apparent. Since many underlying factors could be responsible for the mutagenic and/or teratogenic and carcinogenic mechanisms involved, these preliminary findings do not link polyvinyl chloride production with the increased occurrence of congenital malformations and CNS tumors, but indicate the need for further study of possible contributing factors. (13 refs) 256. CARCINOGENIC, MUTAGENIC AND TERATOGENIC RISKS ASSOCIATED WITH VINYL CHLORIDE. Infante PF, Wagoner JK, Waxweiler RJ Division Surveillance, Natl. Inst. Occupational Safely and Health, Main Post Office Building, Cincinnati, OH 45202 Mutat Res; 41(1). 131 -142 1976 Results of an epidemiological survey of cancer, birth defects and fetal mortality in groups of people with some kind 55 UCC 106378 of exposure to vinyl chloride (VC) are presented. In a cohort of workers occupationally-exposed to VC for at least 5 yr and for whom at least 15 yr had elapsed since the time of initial exposure, observed/expected deaths from all cancers, brain and C'NS cancers, respiratory system cancers, biliary and liver cancers, lymphatic and hematopoietic system cancers were 51, 16,9, 3/0.6, communities with VC polymerization facilities, observed/expected deaths for CNS cancer, leukemia and aleukemia, and lymphomas were 38/24, 47/48.1 and 61/48.7 respectively, and observed/expected rate of congenital malformation (per 1000 live births) was 73/40,8. The feta) mortality rate among wives of workers exposed to VC was assessed by questionnaire and interview surveys to have been 10.1% prior to their husband's exposure to VC; after such exposure, the rate was increased to 16.5%, with the excess in fetal mortality being associated with younger-aged husbands. (30 refs) D. Other Clinical Manifestations of Occupational Exposure to Vinyl Chloride and Related Compounds 257. FURTHER RESULTS IN POLYVINYL CHLORIDE PRODUCTION WORKERS. Lange CE, Juhe S, Stein G. Veltman G Dep. Dermatol., Univ. Bonn, West Germany Ann NY Acad Sci; 246:18-21 1975 The possible nature and extent of occupational damage in 15 employees of polyvinyl chloride (PVC)-processing industries was investigated. In addition, case studies of two former workers of a PVC-producing plant, who had died of malignant tumors, are presented. Dermatological examinations and the following laboratory tests were performed on all 15 workers; complete blood cell platelet count. RBC sedimentation rate, serum electrophoresis, blood urea nitrogen, and Australia antigen. Employed for an average of five years, the workers had complained of sensations of pressure and/or pain in the upper abdomen, frequent dizz iness. cold hands and feet, and increasing weakness in the legs. Clinical changes of Raynaud's syndrome were not observed. Slight to moderate thrombocytopenia (63,000-139,000/microl) was observed in seven patients; increased bromsulphalein retention of 5.2-15.1% at 45 min was noted in another seven patients. Reticulosis of 1,7-4.4% was seen in six patients. Leukopenia (3,250/microl) was seen in one patient, and slight splenomegaly was observed in another patient. Laparoscopy and biopsy results were less distinct, though similar to those observed in PVC-production workers. Despite the small number of workers examined, an increased number of findings were made of a combination of symptoms believed characteristic of vinyl chloride disease. A 38-yr-old man, employed for 12 yr, had a large tumor assigned to the liver and spleen, increased ESR, anemia, and other abnormal lab oratory Endings; surgical laparatomy and biopsies revealed a hemangioendolhelial sarcoma of the liver, and increasing cachexia resulted in death. A 39-yr-old man, employed for 11 yr, had a tumor on the epigastric angle, abnormal laboratory results, a histological diagnosis of hemangioendolhelial liver sarcoma, and resulting death. 258. AN EPIDEMIOLOGIC STUDY OF BLOOD SC REENING TESTS AND ILLNESS HISTORIES AMONG CHEMIC AL WORKERS INVOLVED IN THE MANUFACTURE OF POLYVINYL CHLORIDE. Wyatt RH, Kotchen JM, Hochstrasser DL, Buchanan JW. Campbell DR. Slaughter JC, Doll AH Coll. Med., Univ. Kentucky, Lexington, Ky. Ann NY Acad Sci; 246:80-87 1975 When five cases of liver angiosarcoma were identified among polyvinyl chloride manufacturing workers in a single plant, 882 male employees were screened for blood chemistry abnormalities and illness history The results obtained from 413 of these workers, who had worked for more than one month in the manufacturing unit, were compared with the remaining workers employed in other areas. Two additional cases of angiosarcoma were discovered in the manufacturing workers through this screening. Blood samples were tested for total protein, albumin , calcium , inorganic phosphate , creatine , uric acid , total bilirubin , alkaline phosphatase , LDH , SGOT . cholesterol and creatine phosphokinase . Co lorimetric analysis was performed and enzymatic reactions were read in the 340 nm range. Liver-spleen disease and al lergic problems were observed more frequently in manufacturing workers than other workers while more genitourinary illness was found in the other workers. Whereas more polyvinyl chloride workers had abnormally elevated albumin levels, more workers in other areas had elevated SGOT and alkaline phosphatase levels. After adjusting for age, both albumin and cholesterol level trends with age were found to be different for the two populations. This was not found in any other test. Further study is indicated. 259. SCINTIGRAPHY OF LIVER AND SPLEEN IN VINYL CHLORIDE WORKERS. Biersack HJ, San Luis T. Lange CE. Thelen M, Veltman G, Winkler C * Inst, for Clinical and Experimental Nuclear Medicine, 5300 Bonn-Venusberg. W, Germany Hepatogastroenterol (Stutty); 24(5):357-361 1977 In 152 vinylchloride(VC)-exposed, workers of whom 124 were employed in the polyvinylchloride (PVC) production and 28 in VC-processing plants, liver and spleen imaging was performed using Tc99m-sulphur colloid and Hgl97-BMHP. In 101 (81%) of the 124 workers of the PVC-production plant and in 18 (64%) workers of PVC-processing factories pathological liver and spleen scintigrams were found. The most frequent pathological change in the scintigraphic image was an increase in splenic colloid accumulation, when compared with the liver uptake. Three angiosarcomas of the liver were detected through circumscribed defects of colloid accumulation. Sequential liver scintigraphy was done in 15 cases. In 7 patients with esophageal varices considerable decrease in portal venous blood flow was demonstrated. Scintigraphically detectable changes are sensitive indicators of VC-induced lesions of the liver including liver fibrosis, portal hypertension and angiosarcoma. (Author abstract) (22 Refs) 260. LIVER DISEASE IN POLYVINYL CHLORIDE PRO DUCTION WORKERS - CLINICAL AND LAPAROSCOPIC ASPECTS. Marsteller HJ, Lelbach WK, Muller R, Gedigk P, Lange CE Medizinische Universitatsklinik, D-5300 Bonn-Venusberg, West Germany Leber Magen Darm: 5(5); 196-202 1975 Clinical and histopathological findings in suspected occupationally induced liver and spleen disease among 44 workers engaged in vinyl chloride polymerization are presented. Palpable hepatomegaly and splenomegaly were found in 28 and 16 patients, respectively. A considerable BSP retention, low grade hyperbilirubinemia, and transient elevation in SGOT, SGPT, and alkaline phosphatase were the only remarkable biochemical variables. The majority had pronounced thrombocytopenia, with platelet dysfunction. Hepatic fibrosis with encapsulated vascularization was conspicuous in 35 probands; a nodular liver surface and portal hypertension were also present. Liver histopathology was not conspicuous. Adaptive hepatocyte focalization, nuclear polymorphism and polyploidy, septal fibrosis, focal collagenization of sinusoid walls, and sinusoid endothelial proliferation and atypia were identified. No angiosarcomatous 56 UCC 106379 areas were found in laparotomies of the patients with noncirrhotic portal fibrosis after vinyl chloride exposures ranging between 9 mo and 21-3/4 yr. survived so far for 3, 6, and 8 yr, respectively. With im provements in technology, workers should no longer be exposed to VCM gas levels 5 ppm. (15 Refs) 261. RESULTS OF A STUDY OF 17 CASES OF LONG TERM EXPOSURE TO VINYL CHLORIDE. Muller K.T, Buchter A, Gross R. Bolt W Med. Univ.-Klinik, 5 Koln 41, Joseph-Stelzmann-Str. 9, West Germany Med Welt; 27(t):21-24 1976 A discussion of the physicochemical properties of vinyl chloride (VC) and its toxicology precedes a review of 17 patients with VC intoxication. Liver pathology and positive Raynaud signs occurred in 11/17, esophageal or fundic varices in 8/17, splenomegaly m 8/17, thrombocytopenia in 10/17, and acro-osteolysis in 8/17. Six probands presented with granulo cytopoiesis and marrow stimulation by sternal puncture. Five patients had various changes in antibody composition, and six were leukopenic. All patients were otolaryngologically negative. A suspicion of hepatic hemangioendothelioma in one subject was disconfirmed on laparoscopy, although the patient had elevated serum iron and distal hypalgesia. The subjects were men, with a mean 9-yr VC exposure. 262. PORTAL HYPERTENSION IN VINYL CHLORIDE MONOMER WORKERS. A HEMODYNAMIC STUDY. Blendis LM, Smithe PM, Lawrie BW, Stephens MR, Evans WD Room 112, Univ. Wing, Toronto General Hosp., University Ave., Toronto, Ontario, Canada MSG IL7 Gastroenterology; 75(2):206-211 1978 Hemodynamic studies were performed in five vinyl chloride monomer workers in whom splenomegaly or thrombocytopenia was detected during a screening program at a major chemical plant. Three patients had portal hy pertension and collateral venous circulations, with intrasplenic pressure between 20 and 29 mm Hg and normal wedged hepatic venous pressures, but the gradient between the wedged and free hepatic vein pressures was also increased. Splenic blo od flows were increased in both hypertension and normotensive patients. There was no correlation between the splenic blood flow and the portal pressure or the presence of the portal fibrosis. The portal hypertension associated with vinyl chloride exposure is mainly presinusoidat in type, and may be attributed to an abnormality of the portal vein radicles, or hepatic sinusoids. (Author abstract) (23 Refs) 263. PORTAL HYPERTENSION IN VINYL-CHLORIDE PRODUCTION WORKERS. Smith PM, Crossley IR, Williams DM Dept. Medicine, Llandough Hosp., Penarth, South Glam organ, Wales Lancet; 2(7986);602-604 1976 Portal hypertension was observed in seven patients who had been involved in the production of vinyl-chloride monomer (VCM) for 4-15 yr. The patients were 36-57 yr old at the time of presentation, and they had been exposed to VCM gas for 4-15 yr. Four had hematemesis from esophageal varices, and two of these had had prior splenomegaly and thrombocytopenia. At laparotomy, the liver appeared finely nodular and cirrhotic, but biopsy revealed a noncirrhotic fibrosis in four patients and slight fibrosis in two. Of the four patients who bled from the esophageal varices, one died 2 mo after an esophageal transection from hepatic failure and Escherichia coli septicemia. The other three have done extremely well after end-to-side portacaval shunts. They have 264. PULMONARY CHANGES AMONG VINYL CHLORIDE POLYMERIZATION WORKERS. Lilis R, Anderson H, Miller A. Selikoff IJ Mount Sinai Sch. Medicine, New York, NY Chest; 69(2):299-303 1976 Pulmonary changes uncovered during clinical examinations of three groups of exposed vinyl chloride (VC) polymerization workers are discussed. The examination inc luded chest x-rays, smoking history, a chronic bronchitis questionnaire, and pulmonary function tests, The first group was from a plant known to have high exposure levels to VC and polyvinyl chloride (PVC). The second plant was the first PVC polymerization facility, so that long exposure effects could be expected. The third plant had a relatively low exposure level. In the first group, the overall prevalence of small linear reticular and/or rounded opacities was 22.7%; xray changes increased with length of exposure. In the second group the prevalence of chest x-ray changes was 19.4%. The third group had a much lower prevalence of chest x-ray abnormalities (4.3%). The prevalence of positive smoking history was higher in workers with abnormal chest x-ray films in the first two groups, perhaps indicating a multiple factor effect of smoking and VC-PVC exposure. No significant differences were found between the groups with abnormal and normal chest x-rays with respect to chronic bronchitis or age. Pulmonary function tests showed a relatively high prevalence of obstructive changes, but there was no consistent correlation between abnormal chest x-ray and pulmonary function abnormalities. It is possible that chest x-ray changes and obstructive pulmonary function abnormalities reflect different pathologic processes, the chest x-ray changes being mainly related to parenchymal damage, while the obstructive pulmonary function changes reflect airway changes. 265. PRENEOPLASTIC AND COLLAGENIZING CHANGES IN THE LIVER AND BLOOD CLOTTING IN PVC WORKERS. Bachner U, Etzel F, Muller N, Lange CE, Egli H No affiliation given Thromb Diath Haemorrh; Suppl62:319-326 1976 Sixty-five polyvinyl chloride (PVC) workers with thrombocytopenia were divided into three groups: (A) 31 with unremarkable liver histology; (B) 20 with slight liver fibrosis; and (C) 14 with clear periportal and perisinusoidal fibrosis, stellate (Kupffer) cell proliferation, and atypical stellate cells. Twenty-two blood-clotting tests were carried out and related to pathological and histological findings in the liver. PVC workers with strong collagenizing and preneoplastic changes in the liver showed the lowest number of thrombocytes. Groups B and C showed an increase in platelet factor 3 activity, but this increase was statistically significant in Group C only. Factor VIII activity showed a tendency to rise from Groups A to C. Factor V values were normal in Groups A and B, but slightly decreased in Group C. In ail three, fibrinogen was at high normal or elevated levels, plasminogen was at the lower level of normal, and aggregability was increased. The reaction time in TEG was slightly shorter in Group C than in A or B. Tissue changes in the liver and spleen were related to hindrance of the microcirculation, which could explain the portal hypertension and esophageal varices observed in 10/14 Group C patients. The availability of phospholipids was increased, as shown by the deposit of lipopigments in the sinusoidal and liver cells. (45 Refs) 57 UCC 106380 266. LYMPHOCYTE TRANSFORMATION TESTS IN VINYL CHLORIDE (VC) WORKERS (MEETING ABSTRACT). Fortwengler HP, Dever ME, Tamburro CH, Espinosa E Univ. Louisville Sch, Medicine, Louisville, KY, 40201 Fed Proc: 37(3):362 1978 Previous reports have shown circulating immune complexes in vinyl chloride (VC) workers and a tumorassociated antigen in VC-related liver angiosarcoma. This sug gests immune stimulation by a tissue of plasma antigen induced by or conjugated with VC or a metabolite. In this work, the in vitro lymphocyte reactivity for such antigens was tested in 79 VC workers including 25 with liver abnormalities, and 20 normal individuals having no exposure to VC. The liver angiosarcoma antigen preparation included the tumorassociated antigen and other tissue antigens as shown by im munodiffusion. Stimulation indices (SI) were calculated from cellular incorporation of tritiated thymidine. Mean SI in the normal individuals for antigens of angiosarcoma and normal liver tissues were 4.7 (SE+-I.7) and 3.8 ( + -0.9) and in VC workers. 1.8 (+-0.2) and 2.5 (+-0.3) respectively. Mean SI for phytohemagglutinin and concanavalin A in the normal individuals were 235 (+-35) and 209 ( + -30) and in VC workers 201 ( + -23) and 180 ( + -19) respectively. Thus, these results suggest that VC workers have a decreased lymphocyte response to antigens of liver angiosarcoma and normal liver tissues rather than the hypothesized increased reactivity. This appears to be due to a lower overall lymphocyte responsiveness in these chemical workers. 267. IMMUNOLOGICAL MECHANISMS IN THE PATHOGENESIS OF VINYL CHLORIDE DISEASE. Ward AM, Udnoon S, Watkins J, Walker AE, Darke CS Hallamshire Hosp. Medical Sch,, Sheffield S10 2RX, England Br Med J; l(60l5):936-938 1976 To determine the etiology and pathogenesis of vinyl chloride (VC) disease, immunological and immunochemical investigations performed on 58 workers from a VC polymeriz ation plant showed the presence of circulating immune complexes m 19 out of 28 patients with the disease and in 2 of 30 workers exposed to VC. The immunological data were reviewed in relation to the clinical picture of the disease and to the available evidence on the metabolism of VC. The results suggest that VC disease is an immune complex disorder and that the immune response is initiated by the adsorption of VC or a metabolite, presumably the dioxide, onto tissue or plasma protein, producing either a haptenic group or a conformational change within the protein molecule that escapes tolerance and stimulates B cell proliferation. 268. SPERM COUNT DEPRESSION IN PESTICIDE AP PLICATORS EXPOSED TO DIBROMOCHLOROPROPANE. Glass Rl, Lyness RN, Mengle DC, Powell KE, Kahn E Chronic Diseases Div., Burea Epidemiology, Center Disease Control, Atlanta, GA, 30333 Am J Epidemiol; 109(3):346-35l 1979 Male pesticide applicators who worked with the nematocide dibromochloropropane (DBCP) were examined to determine the possible testicular toxicity from this exposure. Infertility and azoospermia which were first noted among factory workers exposed to DBCP were not observed among the applicators. Sperm count depression, however, was associated with the duration of exposure in the current vr but not with exposure in past yr. The extent of exposure to DBCP in the current y r was also associated with an elevation of serum follicle stimulating hormone (FSH) but not of luteinizing hormone (LH). Sperm count depression was limited to ap plicators involved in irrigation setup work and in the calibration of equipment. These results suggest that the testicular toxicity of DBCP for men may occur in.a shorter period that was previously reported, that the effect may be reversible in men with mild sperm count depression, and that public health measures might be directed at controlling specific application technique. (Author abstract) (12 Refs) VII.PUBLIC HEALTH ISSUES, OCCUPATIONAL SAFETY, AND REGULATIONS CONCERNING VINYL CHLORIDE AND RELATED COMP OUNDS The reader mav also find the following abstracts of interest. 187, 204. 219. 223. 227 269. VINYL CHLORIDE: ITS IMPACT ON OCCUPATIONAL MEDICINE PRACTICE IN IRAN. Aryanpur J Public Health and Occupational Medical Services, Natl. Iranian Oil Company, P. O. Box 1863, Tehran, Iran J Occup Med; 19( 10) 689-692 1977 Measures taken to protect the workers at the Abadan Petrochemical Company in Iran from the carcinogenic effects of vinyl chloride monomer (VCM) and polyvinyl chloride (PVC) are outlined. Since 1971, all workers have been required to undergo periodic medical examinations with an emphasis on liver function. Factors related to liver disease, such as endemic viral hepatitis, low alcohol and cigarette consumption, and dietary habits, are considered. To date, among 43 workers in the VCM and PVC production units, none have evidence of liver dysfunction or angiosarcoma. The threshold limit value for VCM has been set at 25 ppm time weighted av for 8 hr with revisions expected in the future. No leakage to the community has been detected except for some chlorine release. (II Refs) 270. CONTROL STATUS OF VINYL CHLORIDE. Wyatt S United States Environmental Protection Agency, Research Triangle Park. NC, 27711 Tex Rep Biol Med; 37; 145 1978 The Environmental Protection Agency (EPA) listed vinyl chloride as a hazardous air pollutant in December, 1975, and promulgated a national emission standard for it under the authority of section 112 of the Clean Air Act on October 21, 1976. On November 19, 1976, the Environmental Defense Fund (EDF) petitioned the United States Court of Appeals for review ol the standard. On March 24, 1977, EDF and EPA moved to dismiss the court proceedings in view of a settlement agreement requiring EPA to propose certain amendments to the standard. These amendments to the standard were proposed on June 2, 1977, Since that time EPA has received numerous comments from the industry on the proposal. The main issue involved is how carcinogens should be regulated under section 112 of the Clean Air Act. Section 112 of the Act requires that emission standards be set 'at the level which, in the judgment of the Administrator, provides an ample margin of safety to protect the public health from such hazardous air pollutants,' It has not been possible to determine a threshold level of effects for vinyl chloride and it is not certain that such a threshold may be determined in the near future. Therefore, EPA has developed standards which require emission reduction to the lowest level achievable using technological means, (Author abstract) (no Refs) 58 UCC 106381 271. COMMUNITY RISK ASSESSMENT. Downs TD Dept. Biometry, Univ. Texas Health Science Center at Houston, Sch. Public Health, P.O. Box 20186, Houston, TX, 77025 Tex Rep Biol Med; 37:118-122 1978 . A simplified account of a statistical method used to assess community risk from environmental carcinogens is presented. The method is called the linear model and is based on the assumption that the probability, or risk, of developing cancer from exposure to a relatively small amount of carcinogen is directly proportional to that amount. The linear model is il lustrated with data on vinyl chloride inhalation in rats and subsequent development of liver angiosarcoma. Results from these experiments are extrapolated to humans. Rats were intermittently exposed to vinyl chloride at different ambient air concentrations. Exposures to vinyl chloride were maintained for 4 hr/day, 5 days/wk, for a period of I yr. Fol lowing this, surviving rats were housed under normal conditions until death. Whenever a rat died an autopsy was performed to determine if the rat had developed liver angiosarcoma. The proportion of rats with liver angiosarcoma increased steadily as the dose concentrations of vinyl chloride increased. The proportion of rats with liver angiosarcoma seemed roughly proportional to the dose concentration. Use of the linear model was extrapolated to humans to quantify the risk of liver angiosarcoma for residents in the vicinity of a hy pothetical vinyl chloride plant. (6 Refs) 272. OCCUPATIONAL SAFETY AND HEALTH STANDARDS: TOXIC AND HAZARDOUS SUBSTANCES. Department of Labor Occupational Safety and Health Admin., Dept. Labor, Washington, DC Fed Regist; 44(29,Book2):8575-8833 1979 Occupational safety and health standards for toxic and hazardous substances which are applicable to construction are presented. The substances discussed include asbestos, coal tar pitch volatiles, 4-nitrobiphenyl, alpha-naphthylamine, methyl chloroethyl ether, 3,3-dichlorobenzidine and its salts, bischloromethyl ether, beta-naphthylamine, benzidine, 4aminodiphenyl, ethyleneimine, beta-propriolactone, 2acetylammofluorene, 4-dimethylaminoazobenzene, Nnitrosodimethylamine, vinyl chloride, inorganic arsenic, benz ene, coke oven emissions, cotton dust (in cotton gins), 1,2dibromo-3-chloropropane, and acrylonitrile, (no Refs) 273. PUBLIC HEALTH DECISION-MAKING AND THE LEGAL PROCESS IN THE UNITED STATES: THE REGULATION OF VINYL CHLORIDE (MEETING ABSTRACT). Cottinc BR U.S. Occupational Safety and Health Review Commission, Washington, DC The Scientific Basis for the Public Control of Environmental Health Hazards, held by the New York Academy of Sciences in New York, 21-30 June, 1978. The New York Academy of Sciences, New York, New York, 1978. Public health decision-making on the hazards of vinyl chloride exposure occurred in the legal process of the United States through two functional systems. At a federal level 'public-ordering' was accomplished prospectively through authority delegated by Congress to several administrative agencies including the Consumer Product Safety Commission and the Occupational Safety and Health Administration. Investigatory hearings, rulemaking action, and enforcement actions were commenced. Public ordering also occurred retrospectively through Slate worker compensation systems. However, the retrospective systems do not integrate with, or provide data for, prospective action. Moreover, retrospective ordering has a limited sensitivity and field of view due to statutory limitations on the compensability of occupational disease, variable latency periods, access controls on the system, and data visibility. Though limited retrospective data were available on vinyl chloride, prospective regulatory actions necessarily relied on experimental and epidemiological data. Private ordering also occurred on a limited basis, (no Refs) 274. CASE STUDY 3: VINYL CHLORIDE - BEST AVAILABLE TECHNOLOGY. Lassiter DV Occupational Safety and Health Admin.. US Dept. Labor, Washington, DC 20210 Ann NY Acad Sci; 271:176-178 1976 Several tumors have been directly linked with occupational exposure to various carcinogens. Guidelines that determine policy for the regulation of occupational carcinogens, as set forth by the Occupational Safety and Health Administration, are quoted and commented upon. Any regulations should ideally assure minimum risk of cancer to the employee while allowing the manufacturer to remain competitive in a situation in which costly controls must be instituted to restrict exposure. (0 Refs) 275. PRODUCTION AND USE OF VINYL CHLORIDE: IMPACT OF THE LINK BETWEEN VINYL CHLORIDE MONOMER AND CANCER. Pregaglia GF Dept. Res. and Technology, Petrochemical Div., Montedison, Milan, Italy Adv Tumor Prev Detect Charact; 3:209-215 1976 Exposure hazards associated with the production and use of vinyl chloride monomer (VCM) are reviewed along with some possible solutions. VCM has recently been associated with the cancer deaths of production workers exposed to the compound, and the Occupational Safety and Health Administration has recommended that the exposure level be reduced to 1 ppm (averaged over an 8-hr period) starting in 1976. Exposure hazards to VCM in polyvinyl chloride manufacturing plants include: VCM loss from valves and pumps to the ambient air at concentrations ranging from a few to a few dozen parts per million: the need for workers to enter reactor vessels periodically for cleaning; monomer escape from the polymer during storage and processing of the latter; and monomer losses through vent gas streams. Possible means of reducing VCM exposure include: the scale-up to larger reactors, resulting in fewer leaks and easier cleaning; remote control operation and the use of highly sensitive vapor detectors; and new processes for the removal of VCM from vent gases, such as the adsorption of VCM on activated carbon and its regeneration with steam. 276. PUBLIC-HEALTH ROUNDS AT THE HARVARD SCHOOL OF PUBLIC HEALTH. VINYL CHLORIDE: CAN THE WORKER BE PROTECTED? Wegman DH, Peters JM, Jaeger RJ, Burgess WA, Boden LI 665 Huntington Ave., Boston, MA 02115 N Engl J Med; 294(l2):653-675 1976 The toxicology, economic and engineering aspects, and regulation of occupational exposure to vinyl chloride are reviewed. Well before 1974, when three cases of a rare neoplasm, angiosarcoma of the liver, were reported in workers exposed to vinyl chloride over a 20-yr period, there was sufficient evidence to support the presumption of a serious occupational hazard. Toxicological studies as early as 1938 indicated that vinyl chloride has little acute lethal action and 59 UCC 106382 negligible short-term toxicity but is a potent carcinogen in rodents under work-like exposure of 50 ppm and less. Useful toxicologic screening should be carried out in locations other than the workplace and in species other than man. The manner in which workers are exposed to vinyl chloride varies greatly, depending on their occupation in one of three stages: (a) pro duction of vinyl chloride monomer (VCM); (b) reaction of VCM to form polyvinyl chloride (PVC); and (c) the fabrication of plastic products from PVC. Major exposure and the cases of angiosarcoma of the liver occur in the second industrial stage. The most common exposure in the first stage occurs from leaks and faulty equipment maintenance. In the third stage, exposure (1.000 ppm) occurs when workers periodically crawl into the reactor to clean the inside surface. The f ederal occupational standard for vinyl chloride exposure (issued in October 1974), although accepting in principle I ppm as the maximum possible exposure, permits temporary exposures of up to 25 ppm without respiratory protection. 277. VINYL CHLORIDE, PVC AND CANCER. Anonymous Lancet; (7870): 1323-1324 1974 To date. 19 workers throughout the world have rep ortedly developed angiosarcoma of the liver following a history of intermittently heavy exposure to vinyl chloride (VC) over periods of 12-27 years in polyvinyl chloride (PVC) manufacturing plants. Experimental work indicating a relationship between VC and cancer has heretofore gone unheeded because the experimental exposure levels were very high in comparison to those to which PVC workers are exposed and because experimentalists have often warned of hazards where no adequate evidence of real hazard exists. There is no indication that PVC is itself dangerous or that it depolymerizes to produce VC. Unreacted VC which is present in newly manufactured PVC may, however, be released during storage and when the material is heated during the manufacture of PVC products. There have been no cases of angiosarcoma of the liver among PVC processors. In Great Britain, the current upper limit for exposure to VC in PVC manufacturing plant is 50 ppm with a maximum timeweighted average exposure of 25 ppm. In light of evidence that rats develop angiosarcoma of the liver following exposure to 50 ppm VC, these limits may be lowered. More systematic evaluation of industrially used chemicals is needed to determine the carcinogenic risks to workers. 278. VINYL CHLORIDE POLYMERS IN CONTACT WITH FOOD. Schmidt AM No affiliation given Fed Regist; 40( 171 ):40529-40537 1975 Proposed regulations restricting the uses of vinyl chloride (VC) polymers in contact with food, as suggested by the Food and Drug Administration, are reviewed. In general, the proposal permits the continued use of VC polymers in food packaging when the migration potential of VC is diminished, includes an interim regulation for the use of water pipes made from VC poly mers, and prohibits all other use of VC polymers in food-contact articles. The chemical structure and properties of CC and VC homopolymers and copolymers are described. Earlier studies have indicated that polyvinyl chloride (PVC) is insoluble in various solvent systems used to simulate food. However, later migration studies have revealed residual VC from PVC bottles in distilled spirits and wines. No animal feeding studies have established a safe level of consumption when VC is extracted from containers into food. The existence of residual VC in articles made from VC polymers has been found to be related to the manufacturing process and the physical structure of the polymers; a model explaining the VC migration phenomenon is presented. Viewed as a simple diffusion phenomenon, experimental data indicate that while no VC is extractable from packaging materials, the greatest likelihood for migration of VC appears to be from PVC articles intended for one-time use. However, there is little likelihood that residual VC from PVC water pipes will become a component of potable water. Considerable data exist concerning the toxic effects of VC from atmospheric exposures, especially by inhalation and occupational contact; it is also suggested that VC is carcinogenic when ingested. Although a variety of uses of VC polymers in food-contact articles was previously approved, it is now suggested that widespread use be prohibited. It is concluded that no migration of VC from thin plasticized film is expected, nor is any migration expected from jar and bottle cap liners and gaskets. No residual VC reported in beer and soft drink can linings, but VC polymers used as coatings for fresh citrus fruits present the possibility of ingestion. Data also indicate that rigid and semirigid PVC articles intended to contact food may transmit VC to the food they contact. Proposed federal regulations and amendments are presented. 279.SORPTION OF VINYL CHLORIDE BY SELECTED FOOD CONSTITUENTS. Biran D Rutgers Univ., The State University of New Jersey, New Brunswick, NJ Diss Abstr Int B; 38(5):21Q5-B 1977 The association of vinyl chloride (VC) with carcinogenic effects has caused a proposal by the FDA to restrict the use of rigid unplasticized polyvinyl chloride for food packaging. A major area of concern involves the possible interaction between the VC and food. This study deals with parameters affecting the sorption of VC in the gaseous state by selected food constituents and particularly the mechanism of VC sorption by dry casein particles. Partition coefficients for VC distribution between head space and oil, water, oil/water emulsions and casein were determined. It was found that at concentration levels studied the partition coefficients are fairly constant for all cases. Chemical nature of the substrate, starting head space concentrations and temperature were found to be important factors affecting the extent of VC sorption. Dipole moment of the sorbate gas as well as the chemical nature and moisture content of the protein were found to be important parameters determining the sorption of VC by casein. Particle size affects the sorption to a lesser degree. Transient state sorption measurements as well as the inverse gas chromatographic technique showed an active site binding as the major component in the binding interaction between casein particles and VC. An empirical kinetic model for the sorption VC by dry casein particles is proposed. The model suggests a possible clustering effect at the low temperature and most likely a monolayer sorption at the higher temperatures tested. An active site adsorption model is suggested. A correlation between the two models is shown, (no Refs) 280. THE POTENTIAL HEALTH HAZARD OF SUBSTANCES LEACHED FROM PLASTIC PACKAGING. Carter SA Environmental Science and Public Health, Cornell Univ., Ithaca, NY J Environ Health: 40(2):73-76 1977 The health issues surrounding the leaching of four different groups of chemical compounds from plastic products are presented. These groups include: lead and ink from the printed material on plastic wrapping, stabilizers from soft plastic wraps, vinyl chloride from polyvinyl chloride (PVC) 60 UCC 106383 products, and plasticizers from various PVC products. Analysis of PVC peanut oil containers revealed vinyl chloride levels of 0.3-3.3 ppm. Traces of vinyl chloride have been detected in vinegars and alcoholic beverages packaged in PVC bottles. Plasticizers such as di-2-ethylhexyl phthalate and dimethoxyethyl phthalate were found in human and rat blood which had been circulated in PVC tubing, and in tissue samples taken from human patients who had received transfusions of blood stored in PVC bags. These findings and others are discussed in light of pertinent animal and human studies. (28 Refs) 281. VINYL CHLORIDE: A REPORT OF A EUROPEAN ASSESSMENT. Van Esch GJ. Van Logten MJ Natl. Inst. Public Health, Bilthoven, Netherlands Toxicology; 4(l):l-4 1975 A meeting of European toxicologists was held to assess the available toxicological and migration data on vinyl chloride , with special reference to its carcinogenic potential. Because of indications that vinyl chloride induces angiosarcomas in exposed workers, it is considered that 50 ppm vinyl chloride in inspired air is too high to adopt as a Threshold Limit Value. Present industrial exposure levels should be reduced as far as possible, and efforts should be made to eliminate the hazard to operatives of exposure to high vinyl chloride levels during the cleaning of polymerization vessels. Data on the migration of vinyl chloride from polyvinyl chloride (PVC) indicate that PVC used for food and drink containers and wrappings should contain less than 20 ppm vinyl chloride. This is necessary to maintain very low levels of contamination of food. The final solution of the vinyl chloride problem will depend on the evaluation of further data, inc luding (a) epidemiologic studies on vinyl chloride-linked diseases in man, (b) levels of industrial and environmental exposure, (c) effects of po vinyl chloride in animals, (d) investigations to determine if induction of liver tumors by vinyl chloride is preceded by liver dysfunction and cirrhosis, (e) metabolism of vinyl chloride, (0 percutaneous migration of vinyl chloride, '(g) estimated daily dietary intake of vinyl chloride in children and adults, (h) interaction of vinyl chloride with food and drink components, (i) vinyl chloride levels in PVC products, and (j) vinyl chloride levels in potable water and PVC tubing. (9 refs) 282. EXPERIENCE IN INDUSTRIAL EXPOSURE CONTROL. Rowe VK Dow Chem. U.S.A., Health Environ. Res., Midland, Mich Ann NY Acad Sci; 246:306-310 1975 Industrial hygiene methods employed by the Dow Chemical Company provide a model upon which future environmental control systems can be based. The results of toxicological studies of vinyl chloride and vinylidene chloride since 1959 indicated an increased need for monitoring of these substances in the air. Dow widely uses combustionconductivity analysis but gas chromatography and infrared spectrophotometry have also been recently employed. Timeweighted averages of vinyl chloride exposure that an employee would have in a given job can be computed. In 1959, it was established that this average exposure should not exceed 50 ppm vinyl chloride or 25 ppm vinylidine chloride for an eighthour five-day week. Exposure was 1-10.4 ppm for most employees in three monomer plants, but laboratory personnel were exposed to 30 ppm. While levels were only 1-5 ppm for most employees in the polymer plant, some areas showed as high as 150 ppm. Safe levels of any hazardous material must be established and maintained through appropriate engineering and monitoring. Corrective measures should be taken when breakdowns occur and a medical surveillance program must be supported. Vinyl chloride is processed m closed vessels with maximum ventilation, and process automation and remote operation are emphasized. The number of samples for control analysis is minimized, manual collection is reduced by using on-line gas chromatography, and a closed-loop sampling system is used. Polymerization vessels must contain vinyl chloride concentrations of less than 50 ppm before entry for cleaning and air purge must be maintained during the operation. 283. ENVIRONMENTAL CONCERNS BEYOND THE WORKPLACE. Schweitzer GE Off. Toxic Subst.. Environ. Prot. Agency. Wash., D C, Ann NY Acad Sci; 246:296-302 1975 Preliminary investigations were undertaken by the Environmental Protection Agency on vinyl chloride monomer (VCM) and polyvinyl chloride (PVC) activities, especially their migration beyond the manufacturing plants. A nationwide sampling program to determine VCM levels in ambient areas was initiated at ten PVC plants. The need for the proposed Toxic Substances Control Act which would require reporting of industrial data is emphasized. Over 200 million and 50 mil lion pounds of VCM and PVC, respectively, are estimated to be discharged into the air, water, effluents and sludge annual ly. A materials loss of 6% in the PVC production process was reported. Leakage typically occurs during opening of polymerization kettles, transfer, drying and disposal of oversize polymer particles. Increased maintenance has reduced loss to 4% in some plants. Many compounding and fabrication plants are attempting to reduce the amount of monomer associated with the polymer following polymerization since monomer concentration ranges between 500-1,000 ppm and may reach 7,000 ppm. Epidemiological studies of populations near PVC plants and toxicological tests in vivo are necessary in assessing effects of VCM exposure on the nonworker po pulation. In vitro experiments on the significance of impurities in VCM, synergistic effects due to exposure to other chemicals, and metabolic reactions induced by VCM are necessary. The persistence and migration of VCM in the environment should also be studied. Physical properties of PVC must be considered with regard to disposal. Landfill disposal presents problems because long-term PVC stability cannot be guaranteed and HCI is produced when it is incinerated. 284. OCCUPATIONAL DISEASES IN CONNECTION WITH THE MANUFACTURE OF PLASTIC BOTTLES FROM POLYVINYL CHLORIDE. Duport J, Andlauer P, Gattelet M, Chalabreysse J, Archimbaud M, Teulon F, Bertrand R Centre de Recherches de l'LN.R.S,. B.P. 27, 54500 Vandoeuvre-Ies-Nancy, France Arch Mai Prof; 36(4-5):225-241 1975 Because of an outoreak of eczema, the blood and urine from workers in a factory that manufactures plastic bottles from vinyl chloride were examined. The chemical pollutants of the surrounding area, all industrial chemicals and cleansing so lvents, and the heat decomposition of the polyvinyl chloride (PVC) bottles were studied. Over a 6-mo period, 677 blood samples from 159 workers revealed depressed levels of RBC and polynuclear neutrophils, elevated levels of WBC, and variable levels of polynuclear eosinophils. It is concluded that the manufacture of vinyl chloride bottles causes dermatitis and hematological abnormalities characterized by leukocytosis with relative lymphocytosis. Analysis of the air by gas phase chromatography and mass spectrometry revealed only slight traces of aromatic hydrocarbons. No aromatic hydrocarbons were found in the cleansing solvents. Industrial forms of PVC began to decompose slowly between 215 and 220 C; benzene was one of the byproducts of decomposition. Because this 61 UCC 106384 temperature is reached Tor short periods only and because decomposition is slow, it is unlikely that it is a major factor. Neither benzene nor phenol were found in the blood or urine, respectively. There were significant levels of hippuric acid, a possible metabolite of organic toxins, in the urine. Since none of these compounds was considered the cause of the dermatitis or hematological abnormalities, a-phenylindole, the only compound detected in high concentrations in the air, will be studied 285. INDUSTRIAL MEDICINE AND VINYL CHLORIDE. Veltman G. Lange CE Univ Hautklinik, Bonn-Venusberg, 5300 Bonn, W. Germany Berufsdermatosen; 25(2):67-77 1977 After a short review of the history of some other occupational diseases, the institutions and government agencies in West Germany concerned with regulating industrial medicine and occupational hazards are listed. The vinyl chloride (vc) syndrome is recognized as an occupational hazard, and patients are eligible for workmen's compensation. Directions for dealing with vc are described: teaching the employees the hazards of vc, criteria for selecting new employees, regular medical examinations, and recom mendations and rules governing hazard-free working conditions. New products of economic importance should be examined carefully before being commercialized. (4 Refs) 286. THE VINYL CHLORIDE MONOMER HEALTH PROBLEM. Stafford J Plastics Div., ICI, PO Box 6, Bessemer Road, Welwyn Garden City, Hertfordshire AL7 I HD, England C'hem Ind ; 5( 11 ):466-470 1976 Proceedings of the British Plastics Federation conference on 'Vinyl Chloride and Safety at Work' of May 18, 1975 and the conference of the Occupational Health Section of the Royal Society of Medicine of September 12, 1975. were reviewed, us were recent developments on the vinyl chloride health problem in the UK, Europe, and the US. The health hazards associated with polyvinyl chloride manufacture seem to be entirely related to the vinyl chloride monomer (VCM). During 1969-71 the first fibrosarcoma due to VCM was pro duced in experimental animals. Soon after, another investigator found that VCM exposure produced a considerable yield of tumors of many types in the glands and liver of rats exposed to varying dosages, with a linear relationship between the log concentration and the number of tumors. Numerous epidemiological surveys have turned up over 40 cases of angiosarcomas connected with VCM in the world. Nearly all of these cases were autoclave cleaners who experienced high exposures to VCM. Much more statistical data will be necessary to assess the threshold for plant exposure so that a proper perspective can be placed on those standards now being used by manufacturers. (No refs) 287. VINYL C HLORIDE: A CASE FOR THE USE OF LABORATORY BIOASSAY IN THE REGULATORY C ONTROL PROCEDURE. (PP. 1797-1805) Wagoner JK, Infante PF Industry-wide Studies Branch, Div. Surveillance, Hazards Evaluations and Field Studies. Natl. Inst. Occupational Safety and Health, Cincinnati, OH, 45202 Human Risk Assessment, Proceedings of the Cold Spring Harbor Conferences on Cell Proliferation. Vol. 4 (Book C), Hiatt HH, Watson JD, Winsten JA, ed. Cold Spring Harbor, Cold Spring Harbor Laboratory. Origins of Human Cancer.. 583 pp,, 1977. The need for laboratory bioassavs in the regulatory control of chemicals is illustrated using vinyl chloride (VC) as an example. The induction of skin, lung, and bone tumors in rats exposed by inhalation to high VC levels (levels not infrequently approached in the industrial setting) was first rep orted in 1971, 40 yr after VC's commercial introduction but 3 yr before three cases of liver angiosarcoma were reported among workers at a VC polymerization facility. A follow-up study, initiated as a result of these initial angiosarcoma cases, showed a significant excess of deaths from malignant neoplasms among VC workers, compared with US white male death rates. Excess cancer mortality was found for four organ systems: brain and CNS, respiratory system, hepatic system, and lymphatic and hematopoietic system. The cancer risk increased with increasing years of exposure. In the follow-up study, 11/14 confirmed cases of biliary or liver cancer were hepatic angiosarcoma. Among 8 bronchogenic carcinomas, 5 were large-cell undifferentiated and 3 were adenocarcinomas, which is not consistent with distributions previously reported for inhaled carcinogenss. A recent bioassay was predictive not only for the carcinogenicity of VC but also for several of the target organs. VC is mutagenic in microbial test systems, and VC metabolites have induced mutations in mammalian cells. Wives oT VC-exposed men have a significantly high risk of fetal loss. Thus, both mutagenic and carcinogenic assays predicted the potential hazards of VC. (34 Refs) 288. THE POSSIBLE USE OF A BIOLOGICAL EXPOSURE TEST TO DETERMINE HEALTH STANDARDS FOR WORKERS. Teisinger J Srobarava 48, Prague 10, Czechoslovakia Zentralbl Arbeitsmed; 28(I):13-2I 1978 The blood and urine of factory workers exposed to lead, mercury, cadmium, trichloroethylene, benzene, or xylene were tested for metabolites of these agents, and max acceptable values were established. The classification of industrial toxicants in Czechoslovakia corresponds to the classification proposed by the World Health Organization and the International Labor Office and includes the following categ ories: (A) exposure to the toxic agent causes no deleterious effect on the worker; (B) toxic effects are reversible and cause no long-term damage: (C) exposure causes reversible disease; and (D) exposure often causes irreversible damage or death. Lead levels in the blood of persons not continually exposed to lead were measured at 10-40 microg/100 ml blood; the upper limit was established at 70 microg/100 ml blood. Blood lead levels corresponding to category A are 10-40 microg %, B 4070 microg %, C 70-100 microg % and D greater than 100 microg %. The level of coproporphyrin in the urine is an effective indicator of exposure to lead and should not exceed a max value of 0.8-1 mg/liter. For aminolevulinic acid, another indicator of lead exposure, a max urine value was established at 20-25 mg/liter. Max allowable concentrations for mercury and cadmium in urine were established at 0.1-0.3 mg Hg/liter and 10-20 microg Cd/liter. A previously set standard for max concentration of benzene in air (25 ppm) corresponds to a phenol level of 70-100 mg/liter in the urine. Similarly, max air concentrations for toluene and xylene (200 microns/liter air and 200 mg/m', respectively) correspond to 550-850 mg benz oic acid/24 hr and 930 mg toluic acid in urine. Max air concentration for trichloroethylene is 50 ppm. (34 Refs) 289, VINYL CHLORIDE POISONING IN THE USSR: LITERATURE SURVEY. Matufuji H Occupational Health Service Center, Inst. Science Labor, Japan Rodo Kagaku; 54(11):585-592 1978 Among workers exposed to vinyl chloride in western countries about 70 cases of hepatic angiosarcoma have been reported in Western countries, while none have been reported 62 UCC 106385 in the Soviet Union (USSR). However, many cases of chronic vinyl chloride poisoning have been reported in the USSR, while few have been reported in Western countries. The USSR requires poisoned workers to change to work unrelated to vinyl chloride and limited exposure time on the job. Tolerated limits of vinyl chloride are set at 10.7 ppm in the USSR, while the limit has been 500 ppm (1959-1971) to 200 ppm (19711975) in the United States. Inspection of USSR factories between 1953 and 1969 revealed that unacceptable levels (greater than 10.7 ppm) occurred in 2-80% of cases. Exposure regulations and the low tolerance limits in the USSR may explain why patients with chronic vinyl chloride poisoning with characteristic symptoms have not progressed to the point of developing hepatic angiosarcoma. (42 Refs) 290. THE RISK OF CONSUMERS AND PVC WORKERS TO VINYL CHLORIDE. Schlatter CH Institut fur Toxikologie, Schorenstrasse 16, CH-8603 Schwerzenbach bei Zurich Schweiz Med Wochenschr; I06( i9):647-650 1976 The signs and symptoms of vinyl chloride intoxication, the epidemiology of hepatic hemangiosarcoma, the results of animal experimentation, current estimates of occupational risk, and the danger of polyvinyl chloride packaging to the general population are topically reviewed. 291. THE COSTS OF CANCER. Nisbet 1C No Affiliation Given Technol Rev; 78(3):8-9 1975 Social and economic problems arising when a cancercausing substance is identified are discussed, with particular emphasis on the case of vinyl chloride. Prior to the discovery of its hazards, an estimated 100 million pounds a year were lost to the environment during manufacture and a further 4050 million pounds were released through deliberate dispersive uses. Although the most hazardous of these uses - as a propel lant in spray cans - was banned in 1974, and although attempts are being made to reduce manufacturing losses, human exposure continues. The most serious problem is that trace quantities of unpolymerized vinyl chloride are found in polyvinyl chloride. Both nonoccupational and occupational exposure can be hazardous. Although federal law requires strict regulation of occupational exposure to carcinogens, the proposed standard for vinyl chloride offers little or no assurance of safety. 292. NEW IMPETUS FOR PROBE. Anonymous No affiliation given. Chem Week; 114(9): 14 1974 Two former employees of B. F. Goodrich's Louisville, Ky, polyvinyl chloride (PVC) plant were afflicted with angiosarcoma of the liver. This has added new urgency to the investigation into the possible carcinogenicity of vinyl chloride monomer (VCM), At the same time Union Carbide also found that a former employee at its South Charleston, W. Va, PVC plant, died in 1968 of angiosarcoma. Reacting to the Goodrich problem, the Kentucky Safety and Health Standards Board had added VCM to the list of 14 restricted industrial carcinogens issued by OSHA and lowered the employee VCM exposure level from the federal standard to 75 ppm, with a time-weighted average of 50. Because of the lack of solid scientific data on the chemical and the possible huge economic impact, OSHA has not yet issued temporary emergency standards. Investigations pertinent to this matter continue. 293. BATTLE LINES DRAWN ON VINYL CHI OUIDE ISSUE. Anonymous Chem Eng News; 52(8): 16 1974 Controls on the occupational exposure to vinyl chloride or other chemicals used in making the monomer or in polymerizing it to polyvinyl chloride have been requested by the labor unions. These controls include no measurable exposure to the carcinogen, safer work practices, closed systems, use permits, periodic medical and lab tests, plus a cut of residual vinyl chloride in PVC resins to less than 0,01% (100 ppm). The deaths of 4 polymerization section workers at the Louisville, Ky plant of B. F. Goodrich have been attributed to angiosarcoma of the liver. These workers had average exposures of some 19 years to vinyl chloride and 10 years to vinylidene chloride with variable exposure to vinyl acetate, methyl acrylate, ethyl acrylate, methanol and chlorinated so lvents. Angiosarcoma of the liver is a rare occurrence, acc ounting for only 20 to 30 deaths per year in the entire U. S, and can be mis-diagnosed as cirrhosis of the liver. Liver angiosarcomas have been experimentally created in rats inhaling vinyl chloride down to the 250 ppm level. The rats also developed zymbal (sic) glands carcinomas and kidney nephroblastomas, 294. DRINKING WATER AND HEALTH: RECOM MENDATIONS OF THE NATIONAL ACADEMY OF SCIENCES. Blum B Criteria and Standards Div., Office of Water Supply, Environmental Protection Agency, Washington, DC 20460 Fed Regist; 42(132):35764-35779 1977 Recommendations of the National Academy of Sciences concerning the contamination of water supplies with harmful substances are summarized. Four principles relevant to the assessment of risk from long-term exposure to carcinogenic substances at low doses are outlined: (I) effects in animals, properly qualified, are applicable to man; (2) methods to establish a threshold for long-term effects of toxic agents are not in existence; (3) the exposure of experimental animals to high doses of toxic agents is a necessary and valid method of discovering possible carcinogenic hazards in man; (4) materials should be assessed in terms of human risk, rather than as safe or unsafe. Risk estimates have been made for some known water supply contaminants for which data were available. Estimates of lifetime cancer risks (with a 95% upper confidence limit) are 5,1 X 10-', 2.6 X 10-*, 4.4 X 10-'. 1.2 X 10*, 3.1 X 10-*, and 3.7 X I0-' (expressed as risk/ug/liter) for vinyl chloride, dieldrin, kepone, DDT, polychlorinated biphenyls (with data for arochlor 1260), and carbon tetrachloride, respectively. The highest observed concentrations in drinking water of these substances are 10, 8, unknown, unknown, 3, and 366 ug/liter, respectively. The max observed levels of a large number of organic pesticides and other organic contaminants in drinking water are listed, together with recommended acceptable daily intake levels, (no Refs) 295. ASSOCIATION OF BIOREFRACTORIES IN DRINKING WATER AND BODY BURDEN IN PEOPLE. Laseter JL, Dowty BJ Center for Bio-Organic Studies, Univ. New Orleans, New Orleans, LA 70122 Ann NY Acad Sci; 298:547-556 1977 The accumulation and body burden of biorefractories found in municipal water supplies was investigated. The organic chemicals with a mol wt less than 250 are included in this discussion. The major constituents most frequenllv 63 UCC106386 observed in a I liter sample of tap water from New Orleans were benzene, carbon tetrachloride, dichloroethane, bromodichioromethane, and chloroform. The results suggest that many of the halogenated and aromatic organics pass through the treatment plant unchanged. The water treatment process appears to enhance the concentrations of other comp ounds. The results indicate that the quality of water in cities using underground artesian wells is several orders of magnitude lower with respect to concentration of low-mol-wt organic compounds than is that of cities such as New Orleans which relies on the Mississippi River for its water supply. The low-mol-wt organic compounds in the blood of adults and newborn humans are reported and tabulated. A bioaccumulation mechanism is suggested because the concentrations of some of these compounds were found at levels in excess of those commonly reported in drinking water. These compounds are acquired transplacentally by the fetus since carbon tetrachloride, chloroform, and benzene were present in cord blood in quantities equal to or greater than their levels in maternal blood. Drinking water can, therefore, contribute to environmentally derived biorefractories found to accumulate in human tissue. The origin of such compounds must be unequivocally established and their significance for health determined. (14 Refs) 296. VINYL CHLORIDE AND THE PRODUCTION OF PVC (MEETING ABSTRACT). Barnes AW ICI Plastics Div., Welwyn Garden City, Hertfordshire, England Proc R Soc Med; 69(4):277-28 I 1976 The polymerization characteristics of vinyl chloride are described, and the process for producing polyvinyl chloride (PVC) is outlined. Interfaces of exposure of humans to PVC are outlined, and theoretical exposure levels for workers at various stages in the production process and for the average UK civilian are calculated. Average annual dietary ingestion is calculated as 0.0001 g/yr. Atmospheric exposure for polymerization workers has decreased from approximately 1,000 ppni in 1940 to approximately 5 ppm in >975, although certain workers might have been exposed to as much as 3,000 ppm in 1940. The daily dose of the polymerization piant worker who hud been exposed to 1,000 ppm is calculated at 0.36 g/kg, the dose for the polymerization plant worker presently exposed to 5 ppm is 0.0018 g/kg, and that of the average citizen 0.000000004 g/kg. 297. CURRENT INTELLIGENCE BULLETIN 20: TF.TRACHLOROETHYLENE (PERC HLOROETHYLENE). National Institute for Occupational Safety and Health Natl. Inst. Occupational Safety and Health, Cincinnati, OH Current Intelligence Bulletin 20: Tetrachloroethylene (perchloroethylene). Available Through National Technical Information Service, Springfield, Va., as PB278 055/9GA:. DHEW/PUB/NIOSH-78/112, 14 pp,, 1978. Based on a recent study indicating that tetrachloroethylene causes liver cancer in mice, the National Institute for Occupational Safety and Health (NIOSH) recom mends that it is prudent to handle the compound in the workplace as if it is a human carcinogen, while its carcinogenic potential at work is being further evaluated. The recom mended NIOSH tetrachloroethylene exposure limit of 50 ppm and the Occupational Safety and Health Administration standard for occupational exposure of 100 ppm, may not provide adequate protection from potential carcinogenic effects because they were selected to prevent toxic effects other than cancer. It is estimated that about 500,000 workers employed in dry cleaning establishments and other industries are currently at risk of exposure to the compound in the United States. Suggested procedures for control of overexposure to tetrachloroethylene, and guidelines for personal protective equipment and personal hygiene are outlined. (Author abstract) 298. LEGAL DECISIONS AND OPINIONS IN POL LUTION CASES. Hills JP Messenger, Lynch, Hills and Miller, Attorneys and Counselors at Law, Beltsville, MD 20705 Environ Sci Technol; 10(3):234-238 1976 A review is presented of the court histories of a number of legal actions involving the dumping of asbestos-containing waste into Lake Superior, the orders of the Environmental Protection Agency (EPA) to reduce the level of lead in gasoline and ban the pesticide aldrin/dieldrin, and the vinyl chloride standard set by the Secretary of Labor. The indications are that the courts, rather than demanding scientific proof when hearing scientific evidence, are in fact applying a more lenient standard than the traditional legal one: Is it more likely than not that the proposition asserted is true? Thus, in the case of asbestos dumping, the court eventually took action to require the manufacturing company concerned to dispose of the asbestos-containing waste in an alternative fashion, even though it found the possibility of risk to health from dumping to reside only in the realm of respectable medical opinion and not to be an established likelihood. However, because the actual risk to health could not be ascertained, it declined to take drastic or immediate action, but stipulated that the company be given a period of years in which to change its practices. Similarly, the U.S. Court of Appeals for the District of Columbia rejected an appeal of the EPA's ban on aldrin/dieldrin which was based on the argument that there was no proof that the pesticide presented a hazard to human health, since the finding that the material was carcinogenic in several mouse strains could not be extrapolated to humans. The Court noted that certain proof was impossible to obtain, that the burden of prooT regarding the safety of the pesticide rested with the manufacturer, and that a total ban was justified because the concept of threshold levels has no practical significance when carcinogens are involved. The courts seem to be coming to accept that when the risk of harm to large po pulations is at stake, the risk might be very small, and yet justify action. 299. CARCINOGENIC RISK ASSESSMENT: ETHYLENE DIBROMIDE. Ramsey JC, Park CN, Ott MG, Gehring PJ Toxicology Res. Lab., Health and Environmental Res., Dow Chemical Co., Midland, MI, 48640 Toxicol Appl Pharmacol; 47(2):411-414 1979 The incidence of cancer following ethylene dibromide (EDB) exposure predicted by a one-hit carcinogenesis model using parameters derived from a bioassay in rats was compared with that observed in a group of 156 workers employed in EDB production. The parameter estimates were derived from the age-specific incidence of tumor formation in male rats treated with 40 mg/kg/day EDB by gavage in corn oil. The one-hit model estimated that an almost 100% lifetime incidence of cancer should be expected in workers exposed Tor 40 yr to 0,4 ppm EDB at citrus fumigation centers. The duration of exposure to EDB at two locations (at 1 of which employees were also exposed to carbon tetrachloride and chloroform) was determined by work history records of each employee and by an industrial hygiene survey of airborne 64 UCC 106387 concentrations of EDB at one of the locations. Time-weighted av (TWA) concentrations of EDB were conservatively assumed to be 3.0 ppm (23 mg/m(3)). The effect of a different exposure concentration was also conducted at an assumed TWA of 0.9 ppm (6.9 mg/m(3)). The one-hit model predicted either 85 or 54 neoplasms above the normal background incidence at TWa EDB concentrations of 3,0 or 0.9 ppm, respectively, compared with the 8 neoplasms observed to date in both employee groups combined. It is concluded that the use of the one-hit model results in highly exaggerated risk estimates in humans. (8 Refs) 300. D1BROMOCHLOROPROPANE (DBCP) (MEETING ABSTRACT). Legator M. Biles R, Connor T Univ. Texas Medical Branch, Galveston, TX, 77550 The Scientific Basis for the Public Control of Environmental Health Hazards, held by the New York Academy of Sciences in New York, 2l-30June, 1978. The New York Academy of Sciences, New York, New York, 1978. Dibromochloropropane (DBCP) represents another example of a growing list of industrial chemicals where available animal data were disregarded and workers were needlessly exposed to a hazardous substance. This chemical further illustrates the need to establish a meaningful surveil lance program in industry to identify potential carcinogenic and mutagenic agents. Fifteen yr prior to the accidental disc overy of azoospermia in workers, animal toxicity data concerning gonadal effects were availabe. Structurally related compounds were known to effect reproductive systems in the late 1960's. Carcinogenic data were reported in 1973 and 1975. This information was disregarded by both industry and governmental agencies. Furthermore positive mutagenic studies were reported in 1975. Recent investigations indicate that sperm from exposed workers show a significant increase in YY bodies indicating that this chemical may cause segregation errors. Utilization of animal data and industrial populations monitoring should, in the future, prevent exposures to other hazardous chemicals, (no Refs) 301. CRITERIA FOR A RECOMMENDED STANDARDOCCUPATIONAL EXPOSURE TO ALLYL CHLORIDE. NIOSH National Inst. Occupational Safety and Health, Cincinnati, OH Gov Rep Announce Index; 77(I7):84 1977 The recommended standards include an exposure limit of 1.0 ppm as a time-weighted concentration for up to 10-hr work shift in a 40-hr work wk, with a ceiling concentration of 3.0 ppm for 15 min. Provisions are included for sampling, col lection, analysis, pre-employment medical examination, periodic examinations, first-aid, medical records, labeling and posting, personal protective equipment (respiratory protection including respirator requirements, eye protection and skin protection), informing employees, emergency procedures invo lving ally] chloride, control of airborne ally! chloride, storage, handling and general work practices, waste disposal, confined spaces, sanitation, monitoring and recordkeeping. Criteria inc lude the purpose of the standards, biologic effects of exposure (including the extent of exposure, historical reports, effects on humans, epidemiologic study, animal toxicity, correlation of exposure and effect, carcinogenesis, mutagenesis and' teratogenesis), environmental data and analytical methods, basis for previous standards and for the present recommended standard and research needs. (Author abstract) (no Refs) VIII. REVIEWS AND OTHER RELATED STUDIES INCLUDING VINYL CHLORIDE AND RELATED COMP OUNDS Thi' reader mav also lind the following obstructs oj interest: 52. KM. 1JV. 15V, 2HX 302. POTENTIAL HALOGENATED INDUSTRIAL CARCINOGENIC AND MUTAGENIC CHEMICALS. I. HALOGENATED UNSATURATED HYDROCARBONS. Fishbein L National Center Toxicological Res.. Jefferson, AR, 72079 Sci Total Environ; 11 (2); 111 -161 1979 Data on the carcinogenicity and mutagenicity of several of the most industrially significant halogenated unsaturated hydrocarbons are reviewed to assess the nature of their present potential risk. These compounds are vinyl chloride, vmylidine chloride, trichloroethylene, perchloroethylene, chloroprene, trans-l,4-dichlorobutene, hexachlorobutadiene, and ally I chloride. Aspects of their synthesis (primarily in terms of the nature of possible hazardous trace impurities), production vo lumes and use patterns, chemical and biological reactivity and stability, environmental occurrence, and national permissible worker exposure levels are considered. Experimental and human epidemiologic evidence of the carcinogenicity and mutagenicity of the hydrocarbons is reviewed, as are data concerning their in vivo and in vitro metabolism. (302 Refs) 303. POTENTIAL HALOGENATED INDUSTRIAL CARCINOGENIC AND MUTAGENIC CHEMICALS II. HALOGENATED SATURATED HYDROCARBONS. Fishbein L Natl. Center for Toxicological Res., Jefferson, AR, 72079 Sci Total Environ; 11(2): 163-195 1979 The carcinogenic and mutagenic potentials of the industrially significant halogenated saturated hydrocarbons are reviewed. These compounds possess considerable utility as solvents, drycleaning fluids, refrigerants, fumigants, degreasing agents, propellants, and intermediates in the pro duction of other chemicals, textiles, and plastics. They include methyl chloride, methylene chloride, chloroform, carbon tetrachloride, methyl chloroform, 1,1,2-trichloroethane, hexachloroethane, ethyl chloride, and the fluorocarbons. They are discussed principally in terms of their synthesis or occurrence, areas of application, stability, distribution, reactivity, exposure levels, populations at risk, carcinogenicity, mutagenicity, and metabolism. (164 Refs) 304. vinyl chloride-associated liver disease. Berk PD, Martin JF, Young RS, Creech J, Selikoff IJ, Falk H, Watanabe P, Popper H, Thomas L Room 4D-52, Building 10, Section on Diseases of the Liver, Digestive Diseases Branch, Natl. Inst, Arthritis, Metabolism, and Digestive Diseases, N1H, Bethesda. MD 20014 Ann Intern Med; 84(6);717-731 1976 The association of vinyl chloride exposure and liver diseases is reviewed. Polyvinyl chloride has been produced from vinyl chloride monomer for over 40 yr, but recognition of toxicity among vinyl chloride polymerization workers is more recent. In the mid I960's, acro-osteolysis was found in workers involved in cleaning polymerization tanks. In 1974, the same population of workers was found to be at risk for an unusual type of hepatic fibrosis and angiosarcoma of the liver. Two cases of vinyl chloride-associated liver injury, one of hepatic fibrosis and one of angiosarcoma, are presented. The histologic features of these lesions are similar to the hepatic 65 UCC 106388 fibrosis and angiosarcomas resulting from chronic exposure to inorganic arsenicals. Preliminary studies suggest that the toxicity of vinyl chloride may result from formation, during high-dose exposure, of active metabolites by mixed-function oxidases of the liver. Epidemiologic studies indicate an increased incidence not only of liver disease, but also of cancers of the brain, lung, and possibly other organs. (51 refs) 305. TOXIC EFFECTS OF VINYL CHLORIDE. Veltman G, Lange CE, Stein G Univ.-Hautklinik Bonn-Venusberg, D-5300 Bonn 1, W. Germany Hautarzt; 29(4): 177-182 1978 The toxicological aspects of vinyl chloride (VC) are reviewed on the basis of examinations of workers occupationally exposed to VC over long periods of time. The liver histology revealed periportal, septal, and intralobular fibrosis: focal and reticular collagenization of the sinusoid wal ls; hepatocyte degeneration: and activation and proliferation of the sinusoid cells, with cell atypia and transition into angiosarcoma occurring in some cases. The metaphase analysis revealed no remarkable pathological changes: ie, no mutagenic effect of VC. (35 Refs) 3(16. TOXICOLOGY OF VINYL CHLORIDE. Heuse A l.aboratoire de Medecine du Travail et Hygiene du Milieu, Universite Libre de Bruxelles, Brussels, Belgium Brux Med: 58(1): 13-34 1978 A review of the literature on vinyl chloride (VC) traces the history of the toxicity of the compound. Also reviewed are the toxic effects of polyvinyl chloride (PVC), relative risk of industrial exposure to VC and PVC, and potential danger of exposure of the general population to PVC. (196 Refs) 307. INDUSTRIAL HAZARDS DUE TO VINYL CHLORIDE. Huhlet P Ecole de Sante publique de la Faculte de medecine. Universite libre de Bruxelles, rue Belliard 100, 1040 Brussels. Belgium Arch Belg Med Soc: 33(2):73-89 1975 Various aspects of vinyl chloride are reviewed: its physical and chemical properties, methods of synthesis, means of identification, uses, evidence of toxic effects, and industrial risks due to exposure. Because of its toxicity, greater efforts should be made to reduce the concentration in factory air, and workers exposed to the chemical should be examined often. More detailed studies of vinyl chloride and other toxic chemicals should be undertaken. 308. NEW DISCOVERIES AND OBSERVATIONS ON THE PROGRESS OF VINYL CHLORIDE DISEASE (MEETING ABSTRACT). Veltman G. Lange CE, Stein G Bonn, W. Germanv Z Hautkr; 52(6): 1% 1977 The symptomatology of vinyl-chloride (VC) disease with special attention to newer discoveries and the danger to the worker in VC-related industries are discussed. Various periodic checks on skin and bone changes, thrombocytes and liver functions are available. The preventive measures that have arisen from this knowledge have generally removed the immediate threat at the factory, (no Refs) 309. AN OVERVIEW OF THE VINYL CHLORIDE HAZ ARD IN CANADA. Basuk J, Nichols A Science Council Canada, Ottawa, Canada Chem Can; 29(7):24-38 1977 The effects of occupational exposure to vinyl chloride monomer (VCM) are emphasized in this review, which also includes a discussion of the properties and processing of VCM. VCM came to attention as a health hazard in 1973, when three cases of a rare form of liver cancer, angiosarcoma, were rep orted in workers from a VCM factory. Since then, 48 victims have been identified; others who may have died from angiosarcoma are unknown because of difficulty in diagnosing the disease. VCM has chronic effects on human beings at high levels of exposure. It causes a specific occupational disease known as acro-osteolysis, in which there is both Raynaud's syndrome and sclerodermiform lesions. In animal studies VCM has produced a wide range of tumors in rats, mice, and hamsters; Zymbal gland carcinomas, nephroblastomas, angiomas and angiosarcomas of the liver and other sites, trichoepitheliomas, hepatomas, lung adenomas, mammary adenomas and carcinomas, and lymphomas. Recent findings suggest that mammary carcinomas can be induced in lab oratory animals at less than or equal to 1 ppm. Efforts to control the adverse health effects of VCM include the setting of standards for occupational exposure to VCM, improved manufacturing techniques to minimize VCM exposure and residual VCM in polyvinyl chloride resin, and increased research on the epidemiology of VCM-related diseases and on diagnosing preangiosarcoma tumors. (65 Refs) 310. CARCINOGENICITY OF VINYL CHLORIDE: CURRENT RESULTS. EXPERIMENTAL EVIDENCE. Maltoni C Inst. Oncology and Bologna Tumour Centre, Bologna, Italy Adv Tumor Prev Detect Charact; 3:216-237 1976 Partial results are presented from a series of experiments designed to study the effect of vinyl chloride (VC) administered through different routes at different concentrations, for varying periods of time, by continuous or intermittent treatment, on animals of different species (rats, mice, hamsters), strains (Sprague-Dawley and Wistar rats), sex, and age (adults, newborns, embryos). A complete autopsy was made on each animal, which was kept under observation until spontaneous death. Histological examinations were performed on Zymbal glands, interscapular brown fat, salivary glands, tongue, lungs, liver, kidneys, spleen, stomach, different segments of the intestine, bladder, brain, bones of the legs and feet, and any other organ with pathological lesions. Animals exposed to the highest doses (30,000 and 10,000 ppm), with or without tumors, were examined radiologically. When given by inhalation, VC produced the following tumors: In rats, Zymbal gland carcinomas, nephroblastomas, angiosarcomas, mammary carcinomas, and forestomach papillomas: in mice, lung adenomas, mammary carcinomas, angiosarcomas and angiomas of the liver and other sites, skin epithelial tumors, and forestomach papillomas; and in hamsters, liver angiosarcomas, skin trichoepitheliomas, melanomas, forestomach papillomas, acanthomas, hepatomas, and lymphomas. The response was affected by the length of exposure and by the strain. The onset of tumors in the offspring of breeders exposed during pregnancy for 7 days suggests a transplacental effect. When given by stomach tube at high doses, VC induced angiosarcomas of liver and other sites and Zymbal gland carcinomas in rats. The doses pro ducing these tumors, however, were extremely high when compared to possible human exposure. Thirty cases of liver 66 UCC 106389 angiosarcoma have been identified among workers of VCPCV (polyvinyl chloride) industries in the US and several European countries. The majority of cases did not occur until 15 yr or more after the first exposure to VC. An excess mortality for cancers of the respiratory tract, blood-forming tissues, and brain has also been observed among workers of VC polymerization plants in the US. It is concluded that VC carcinogenesis has shown the value of experimental bioassays in predicting oncogenic risks. (7 refs) 311. CHLOROPRENE (2-CHLORO-1,3-BUTADIENE) WHAT IS THE EVIDENCE FOR ITS CARCINOGENICITY? Haley TJ Dept. Health, Education, and Welfare, Food and Drug Admin., Natl. Center Toxicological Res., Jefferson, AR, 72079 Clin Toxicol: 13(2): 153-170 1978 The biochemistry, metabolism, and toxicology of chloroprene (CP: 2-chloro-1,3-butadiene) were reevaluated to establish whether it is a potential carcinogen. The po LD(50)'s of CP in mice and rats are 260 and 251 mg/kg, respectively. The pathologic changes in these animals included hem orrhages and dystrophic changes in the CNS, lungs, kidneys, and spleen. Chronic inhalation of CP by dogs resulted in changes in higher nervous activity, the nerve cells of the cerebral cortex, and the brain vasculature. Blood histamine increased and histaminase activity decreased in 103 Soviet workers exposed to CP, and the changes were related to duration of exposure. During chronic CP intoxication, there was dysfunction of both the CNS and peripheral nervous system, particularly, the cholinergic branch. Cytogenetic analysis of somatic cells from exposed workers aged 23-59 yr revealed both chromosome and chromatid aberrations. Im munization of 208 CP workers with typhoid vaccine produced low immunologic reactivity and no increase in phagocytic activity. In mice, the growth of transplanted Crocker's sarcoma was accelerated by sc CP injection. During 19561970, 137 cases df skin cancer were diagnosed in 24,989 Soviet patients; CP workers had the highest skin cancer incidence (21/684), followed by persons working with CP derivatives (38/2,250). In the same period, 87 lung cancers were found in 19,979 workers; 18 of the patients had direct and prolonged exposure to CP and 16 had a history of exposure to CP latexes. Worldwide epidemiology studies should be undertaken to validate the Soviet reports of CP carcinogenicity in humans. Additional metabolic studies are also necessary to define the neurohumoral mechanism of CP action. (149 Refs) 312. VINYL HALIDES: CARCINOGENICITY. VINYL BROMIDE, VINYL CHLORIDE, AND VINYLIDENE CHLORIDE. Bahlman LJ, Alexander V, Infante PF, Wagoner JK, Lane JM, Bingham E Natl. Inst. Occupational Safety and Health, 5600 Fishers Lane, Rockville, MD, 20857 Am Ind Hyg Assoc J; 40(4):A-30-A-40 1979 Laboratory studies demonstrating the carcinogenicity and mutagenicity of vinyl chloride (VC), vinylidine chloride (VDC), and vinyl bromide (VB) are reviewed, together with studies demonstrating the carcinogenicity and mutagenicity of VC in humans. Liver angiosarcomas have been induced in rats or mice by vinyl halide concentrations of 25-55 ppm. It is recommended that VB and VDC be considered in the workplace as potential carcinogens to humans and controlled with the same degree of prudence as VC. (35 Refs) 313. TRICHLOROETHYLENE. (PP. 263-276) 1ARC Working Group IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. Cadmium, Nickel, Some Epoxides, Miscellaneous Industrial Chemicals and General Considerations on Volatile Anaesthetics. International Agency for Research on Cancer. Lyon France, Vol II. 1976. The chemical and physical data; production, use, occurrence, and analysis; and biological data relevant to the evaluation of carcinogenic risk to man of trichloroethylene are examined. In the US, approx 90% of the trichloroethylene pro duced is used for vapor degreasing of fabricated metal parts. Gastric intubation of 2.4 or 1.2 g/kg body wt trichloroethylene 5 x/wk in male B6C3F mice and of 1.8 or 0.9 g/kg in females induced hepatocellular carcinomas in 30/98 mice given the low dose and in 41/95 mice given the higher dose. Hepatocellular carcinomas occurred in 1/40 control mice. Gastric intubation of either 1.0 or 0.5 g/kg of the compound in both sexes of Osborne-Mende! rats, 5 x/wk for an unspecified period, pro duced no hepatocellular carcinomas. (61 Refs) 314. A REVIEW ON THE TOXICITY OF TRACE AM OUNTS OF TETRACHOLOROETHYLENE IN WATER. Utzinger R, Schlatter C Inst. Toxicology, Swiss Federal Inst. Technology, CH- 8003 Schwerzenbach, Switzerland Chemosphere; 6(9):517-524 1977 The toxicity, metabolism, mutagenicity, carcinogenicity, and environmental concentrations of tetrachloroethylene (C2C14) are discussed. The ingestion or inhalation of 50 mg/day C2CI4 is considered acceptable; therefore, the trace amounts of C2CI4 in air and drinking water are not a serious health problem. Mice exposed to the compound for 12 mo (300 or 600 ppm, 6 hr/day 5 days/wk) developed liver cancers, but these results could not be extrapolated to humans. (22 Refs) 315. VINYLIDENE CHLORIDE: A REVIEW OF THE LITERATURE. Haley TJ US Dept. Health, Education and Welfare, Food and Drug Admin., Natl. Center Toxicological Res,, Jefferson, AR Clin Toxicol; 8(6);633-643 1975 The literature concerning the chemistry, industrial hy giene, and toxicology of vinylidene chloride (VC) is reviewed. The po administration of 2 mg/kg VC to rats decreases liver glucose-6-phosphatase and increases liver alkaline phosphatase and tyrosine transaminase and also plasma alkaline phosphatase and alanine transaminase. The threshold limit value of VC is 5 mg/cubic meter. The liquid or the vapor form is highly irritating to the eyes and skin, but the chemical is most dangerous when inhaled. In rats, the signs of toxicity upon inhalation include continuous blinking, lacrimation, nasal irritation, roughened coat, excessive salivation, accelerated respiration, gasping, tremors, convulsions, inco ordination, prostration, and narcosis. Human exposure to VC results, within 8-30 hr, in irreversible lesions of the trigeminal nerve. VC exerts its primary effects on the CNS, peripheral nervous system, and, particularly, the liver and kidneys. More detailed long-term studies are needed lo determine the possible carcinogenicity of this compound. (38 refs) 67 UCC 106390 316. ETHYLENE DIBROMIDE. (PP. 195-209) IARC Working Group IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Man: Some Fumigants, the Herbicides 2,4-D and 2,4,5-T, Chlorinated Dibenzodioxms and Miscellaneous Industrial Chemicals. Lyon. International Agency for Research on Cancer, Vol. 15, 1977 The experimental evidence on the carcinogenicity of ethylene dibromide is reviewed. When the compound was administered po to (C57BI x C3H)F1 mice and OsborneMendel rats, squamous cell carcinomas of the forestomach were noted. Ethylene dibromide injected ip in rats induced damage to the spermatogenic cells: however, no data on embryotoxicity or teratogenicity are available. Mutations were induced in Neurospora crassa and Drosophila melanogaster receiving the compound. Ethylene dibromide has been shown to be toxic to man, but no data are available on its carcinogenicity in humans. (62 Refs) 317. REVIEW OF SELECTED LITERATURE ON ETHYLENE DIBROMIDE (EDB). Kover FD Environmental Protection Agency, Office Toxic Substances, Washington, D.C. Gov Rep Announce (US); 76(26): 157 1976 A review of some of the literature on ethylene dibromide is presented. Among the topics discussed are: its uses, properties, production, environmental aspects, sampling and analysis methodology, emission estimates, biological and toxicological considerations, mutagenic potential, repro ductive effects, carcinogenic activity, regulations, and substitutes, (no refs) 318. OCCUPATIONAL CHEMICAL CARCINOGENESIS: NEW FACTS, PRIORITIES AND PERSPECTIVES. (PP. 127-149) Maltoni C Environmental Pollution and Carcinogenic Risks. Lyon, International Agency for Research on Cancer, IARC Scientific Publications No 13, INSERM Symposia Series, vol. 52. 1976. The results of experiments on the carcinogenicity of vinyl chloride (VC) are presented in 18 tables as part of a paper cal ling for a more active approach to the problem of environmental oncogenesis. VC was inhaled at doses from 30,(XX) ppm to 1 ppm for 4 hr/day, 5 days/wk for 52 wk by rats (Sprague-Dawley, Webster), mice (Swiss), and hamsters (Chinese): 50 ppm was the dividing dose in carcinogenic potential, and a variety of cancers resulted from the higher dosages. VC was also administered by inhalation at 10,000 ppm and 6,000 ppm, 4 hr/day for I wk. The resulting cancers were monitored in the animals and their offsprings. In ad dition. VC was ingested at 50.00, 16.65, and 3.33 mg/g body weight, once daily, 4-5 days/wk for 52 wk; liver angiosarcomas were the predominant resulting neoplasms. Lower dosages of I, 0.3, and 0.03 mg/g body weight produced no tumors. Subcutaneous administration into rats of 30 mg of compound in I cc of water of chromite, neochromium, chromium allumen, lead chromate, molybdenum orange, cadmium sulphide, iron oxide, zinc chromate, and titanium oxide resulted in rhabdomyosarcomas and fibrosarcomas in all cases but chromite, iron oxide (red), titanium oxide, and zinc chromate. Adriamyctne was tested in rats by sc injection of 2 mg in I cc olive oil. Tumors resulted in 35% of females and 30% of males after an average latency of 31-32 wk. Plans of experiments to test the oncological effectiveness of styrme, vinvlidene chloride, and acrylonitrile are also given in tabular form. (14 Refs) 319. CANCER AND CONGENITAL ANOMALIES ASSOCIATED WITH ANESTHETICS. Corbett TH US Veterans Admin. Hosp., Ann Arbor, MI 48105 Ann NY Acad Sci; 271:58-66 1976 Certain anesthetics in general use may be carcinogenic, embryolethal, teratogenic, and/or mutagenic. Spontaneous abortion rates as high as 38% have been reported among nurse anesthetists, compared to a rate of 9% among general duty nurses, and 16.4% of 434 children born to nurse anesthetists who had worked during pregnancy had birth defects, compared to only 5.7% of 261 children born to nurse anesthetists who had not worked while pregnant. Three studies have indicated an increased cancer risk in operating room personnel; a recent survey involving 50.000 such personnel and 30,000 non-operating room female medical personnel controls revealed an increased prevalence of cancer in the former, ranging from 1.3 to 2 times the control rate. Halogen ether anesthetics such as isofiurane, methoxyflurane, and enfiurane are similar in chemical structure to the proved carcinogen bis(chloromethyl) ether, and trichloroethylene is likewise similar to the carcinogen vinyl chloride. Isofiurane has been shown to be carcinogenic in mice. When pregnant Swiss/ICR mice were exposed to 0.5% isofiurane for 2 hr on days 12, 14, 16, and 18 of pregnancy and the offspring were exposed to 0.1% isofiurane every other day from age 5 days for 25 exposures, 10/37 male offspring killed after 15 mo had hepatic neoplasms, with three animals having multiple tumors. No neoplasms were observed among 23 control animals. Isofiurane, and the structurally similar halogenated ether and alkane anesthetic agents, must be regarded with suspicion and studied for carcinogenic properties as soon as possible. 320. GENETIC EFFECTS ASSOCIATED WITH INDUSTRIAL CHEMICALS. (PP. 100-113) Wagoner JK, Infante PF, Brown DP Industrywide Studies Branch, Div. Surveillance, Hazard Evaluation and Field Studies, Natl. Inst. Occupational Safety and Health, Cincinnati. OH Proceedings Conference on Women and the Workplace. June 17-19, 1976, Washington, D.C. Society for Occupational and Environmental Health, Washington, DC, 364 pp,, 1977. The carcinogenicity and mutagenicity of various chemicals found in the workplace are reviewed, with emphasis on their genetic effects. Women have been excluded from workplaces where vinyl chloride (VC) may be inhaled, but studies have also shown an increased fetal death rate in the offspring of men exposed to VC. Two structural analogs of VC, vinylidene chloride and trichloroethylene, which have wide industrial use, have been shown to be carcinogenic in rodents and mutagenic in microbial and plant assays. However, human data for both are lacking. Functional disruption of spermatogenesis occurred among men occupationally exposed to chloroprene (2-chlorobutadiene) for less than or equal to 10 yr, and morphological disruption of spermatogenesis occurred among men exposed for greater than 10 yr. Wives of workers exposed to chloroprene have a threefold excess of miscarriage. (38 Refs) 321. EFFECTS OF ENVIRONMENTAL CHEMICALS ON THE GENETIC REGULATION OF MICROSOMAL ENZ YME SYSTEMS. Nebert DW, Levitt RC. Orlando MM, Felton JS Room I3-N-234, Bldg. 10, Natl. Inst. Child Health an1 Human Development. Bethesda, MD 20014 Clin Pharmacol Ther; 22(5,part2):640-658 1977 Examples of the interaction of environmental carcir (polycyclic hydrocarbons, halogenated hydrocarbc 68 UCC 106391 acetylaminofluorene and acetaminopen) with the genetic regulatory system controlling the monooxygenase response are presented. Studies have indicated that because of the small number of genes involved in a chemical's metabolism, an individual's response to a given environmental chemical can vary, even among siblings. (79 Refs) 322. INDUSTRIAL MUTAGENS AND POTENTIAL MUTAGENS. I. HALOGENATED ALIPHATIC DERIVATIVES. Fishbein L Natl, Center Toxicological Res., Jefferson, AR 72079 Mutat Res; 32(3/4):267-307 1976 Many industrially and environmentally significant halogenated aliphatic derivatives are discussed in terms of their use patterns, residue levels and distribution patterns, chemical and physical properties, and metabolism, Halogenated hydrocarbons are discussed in general, while mutagenic and potential mutagenic halogenated aliphatic derivatives are specifically discussed. These derivatives include vinyl chloride, vinylidene chloride, trichloroethylene, tetrachloroethylene, ethylene dichloride and dibromide, chloroprene, chloroform, carbon tetrachloride, fluorocarbons, epichlorohydrin, 2-chloroethanol, and haloethers. (242 refs) 323. VINYL CHLORIDE AND VINYL BENZENE (STYRENE) - METABOLISM, MUTAGENICITY AND CARCINOGENICITY. Vainio H Dept. Industrial and Toxicology, Inst. Occupational Health, Haartmaninkatu 1, SF-00290 Helsinki 29, Finland Chem Biol Interact; 22( 1): 117-124 1978 The metabolism, mutagenicity, and carcinogenicity of vinyl chloride, (VC) and vinyl benzene (styrene) are reviewed briefly. VC and styrene are mutagenic in bacterial lest systems. Drosophila, yeast, and mammalian cells. The mutagenicity of VC in bacterial test systems depends, at least partially, on metabolic activation by microsomal enzymes, Chloroethylene oxide, the primary biotransformation product of VC, is a potent mutagenic and alkylating agent. Styrene is mutagenic to Salmonella typhimurium, but only after metabolic activation, whereas styrene oxide, its primary biotransformation product, is mutagenic to Salmonella without activation. In several studies, an excess of chromosome aberrations (compared with controls) was found in the lymphocytes of workers exposed to VC monomer. In ad dition, an excess fetal loss occurred among women whose husbands are heavily exposed to VC. Workers exposed to styrene also had increased chromosome aberrations in their* lymphocytes. Both chloroethylene oxide and styrene oxide hind covalently to cellular macromolecules. VC is carcinogenic 1 rats (skin, lung, bone, liver) and humans (liver, brain, lung, 1 lymphatic tissue). Styrene oxide was a weak carcinogen \ ' applied to mouse skin. Styrene is currently being tested mals. These findings raise concern about the possible \ '(sks of VC and styrene to humans. (55 Refs) T FINDINGS ON THE CARCINOGENICITY SATED OLEFINS. * fffiv and Tumor Center, Bologna, Italy Perspect; 21:1-5 1977 effecting the carcinogenicity of vinyl uiylidene chloride (VDC) are discussed. Impounds have very similar molecular tdely different biological effects. VC is a multipoiemul carcinogen, but VDC has produced tumors only in the murine kidney. Dose, concentration, length of treatment, route of administration, and animal species, strain, sex, and age also significantly affect the neoplastic response. These factors may alter the metabolic pathway of the test compounds. The carcinogenicity of VC and VDC is due to their active metabolites, probably epoxy derivatives. Animal species, strain, and sex greatly affect the production of active metabolites of VDC. In Swiss mice and Sprague-Daw ley rats, there is a parallelism between the toxic and carcinogenic effects of VDC in relation to species and sex. Confirmation of this parallelism in other strains would indicate new routes for establishing experimental animal models, and for understanding the mechanisms of action of many organic carcinogens. 325. MUTAGENIC AND CARCINOGENIC RISKS ASSOCIATED WITH HALOGENATED OLEFINS. Infante PF Industry-Wide Studies branch, Div. Surveillance, Hazard Evaluations and Field Studies, Natl. Inst. Occupational Safety and Health, Center Disease Control. Dept. Health, Education and Welfare, Cincinnati, OH, 45202 Environ Health Perspect; 21:251-254 1977 The mutagenicity and carcinogenicity of vinyl chloride (VC), vinylidene chloride, trichloroethylene, perchloroethylene, and chloroprene are reviewed. Of the first four compounds, all except perchloroethylene have been shown to be mutagenic in test systems, and all have induced tumors in experimental animals. Studies with chloroprene have indicated that it causes sterility in male mice and rats. Rat studies have also indicated that concentrations ranging from 0.04 to 1.0 ppm result in a dominant lethal effect, affect sperm, cause testicular atrophy, and cause chromosomal aberrations in bone marrow cells. Human studies have indicated both an increase in chromosomal aberrations and a decrease in mobility of the sperm of exposed workers. Several studies have indicated an excess of lung and skin cancer in exposed workers, and one report confirmed a case of angiosarcoma of the liver in a worker with extensive exposure to finished polychloroprene. Studies with VC have indicated that in ad dition to angiosarcoma, there appears to be a dose-response relationship between exposure and the induction of mammary cancer in rats. A study of women employed in the VC industry indicated a 38% excess of breast cancer in those exposed. These findings could indicate another site of VC carcinogenesis. (29 Refs) 326. SESSION II. CARCINOGEN SCREENING: OBSTACLES AND OPTIONS. IN VITRO TESTING OF ENVIRONMENTAL MUTAGENS/CARCINOCENS. (PP. 27-33) Ames BN Berkeley, CA, 94720, Univ. California Structural Correlates of Carcinogenesis and Mutagenesis A Guide to Testing Priorities? Proceedings of the Second Food and Drug Administration Office of Science Summer Symposium held in Annapolis, 31 August-2 September 1977. Office of Science, FDA, Annapolis, MD, HEW Publication No (FDA)78-I046, 241 pp,, 1978. The development, accuracy, and recent applications of the Ames Salmonella mutagenesis test are discussed. Chemicals tested for mutagenicity and carcinogenicity have included flame retardants in children's pajamas, pesticides, vinyl chloride, and dibromo compounds in citrus-flavored soft drinks. (9 Refs) 69 UCC 106392 327.THE RELEVANCE OF DOSE-DEPENDENT PHARMACOKINETICS IN THE ASSESSMENT OF CARCINOGENIC HAZARD OF CHEMICALS. (PP. 187203) Gehnng PJ, Watunabe PG, Young JD Toxicology Res. Lab., Health and Environmental Res., Dow Chemical. Midland, ML 48640 Incidence of Cancer in Humans, Proceedings of the Cold Spring Harbor Conferences on Cell Proliferation. Vol. 4, Hiatt HH, Watson JD, Winsten JA, ed. Cold Spring Harbor, Cold Spring Harbor Laboratory, Origins of Human Cancer.. 602 pp., 1977. The use of data obtained after the lifetime daily administration of max tolerated doses of an agent to animals (usually rats or mice) to predict the hazard of low-level exposure to the agent is not justified. Changes in the fate of the chemical or in the metabolic status of the animals preclude the use of routine statistical processes to predict hazards from low doses. Metabolic thresholds may lead to a disproportionate increase in toxicity, including carcinogenesis. This concept is Illustrated by pharmacokinetic studies with 1,4-dioxane and vinyl chloride (VC). The marked dose-dependent fate of dioxane and the strong metabolic induction by high doses, together with toxicologic data, negate extrapolation of highdose carcinogenesis to predict the hazard of low doses. In rats, cancer induction occurs only at doses sufficient to cause marked pathology, metabolic alteration, and even death. These effects, including carcinogenesis, are correlative with the dose-dependent fate of dioxane. In light of this correlation, the hazard of low-level exposure to dioxane appears to be nil. Studies indicate that VC is metabolized by at least two different pathways. The fate of VC changes with dose because the primary pathway for its metabolism is saturated at high doses or exposures. However, both pathways for the metabolism of VC produce reactive metabolites that lead to the same end products. A correlation appears to exist between doses of VC that cause tumors and those that saturate metabolic or detoxifying pathways. There is a threshold of exposure ip rats, in which the physiologic defense mechanisms remain fully operative. Thus, dose-dependent alterations in the fate of chemicals must be considered when using toxiciological or carcinogenic data obtained at high doses to assess the haz ard of low doses. (21 Refs) 328. REVIEW OF ANIMAL STUDIES (MEETING ABSTRACT). Williamson KS ICI Ltd.. Central Medical Group. Fulshaw Hall, Wilmslow, Cheshire, SK9 1QB. England Proc R Soc Med: 69(4):281-283 1976 The author reviews several studies that have been conducted on animals exposed to vinyl chloride gas. It appears that the proportion of the dose retained and metabolized is greater following small doses than large ones. The amount that is inhaled is eliminated in three ways: unchanged from the lungs, in the carbon dioxide from the lungs, and by the excretion of metabolites in the urine. Although many studies are incomplete, there is substantial evidence to indicate that vinyl chloride is oncogenic. 329. MUTAGENICITY TESTS IN CHEMICAL CARCINOGENESIS. (PP. 229-240) Bartsch H Unit of Chemical Carcinogenesis, International Agency for Research on Cancer, 150 cours Albert Thomas, 69008 Lvon France, Environmental Pollution and Carcinogenic Risks. Lyon, International Agency for Research on Cancer, IARC Scientific Publications No 13, INSERM Symposia Series, vol. 52, 1976. The use of mutagenicity tests in the assessment of chemical carcinogenicity is assessed. The validity of this ap plication is demonstrated with the vinyl chloride experiments in rats, mice, human liver, and Salmonella typhimurium and the N-nitrosamine activities in rats, human liver, and S. typhimurium. The usefulness of mutagenicity tests in predicting possible carcinogenic effects of chemicals in man is valid only if the short-term bioassay is corroborated by data from long-term tests in experimental animals and is then taken together with epidemiologic studies. Mutagenicity tests, therefore, although effective in predicting the carcinogenic potential of chemicals, are not able to indicate organ and species specificity of the carcinogenic activity of the chemical or correlate mutagenic potency with carcinogenic potency. (50 Refs) 330. OCCUPATIONAL CANCER DISCUSSED AT NEW YORK CONFERENCE. Englund A, Holmberg B Landsorganisationen, Sweden Lakartidningen; 72(38):3487-3488 1975 The proceedings of a conference on occupational cancer organized by the New York Academy of Sciences in co operation with National Cancer Institute and NIOSH (held on March 24-27, 1975, New York) are reviewed. Increased incidence of lung cancer was found among workers exposed to very small glass-fibers in glass-fiber manufacturing plants. A suspected relationship between asbestos exposure and cancer of the larynx was verified. Increased incidence of skin and lung cancer was found among workers exposed to chloroprene (Neoprene). Vinyl chloride, trichlorobenzene, and benzoyl chloride were found to be carcinogens. Increased incidence of lung and kidney cancer was observed among coke oven operators. The high time requirements and the high expenses involved in carcinogenicity testing, and the correlation existing between carcinogenic and mutagenic properties justifies short term mutagenicity tests for screening for carcinogenic substances. Because of the many factors involved in the carcinogenic response, a zero exposure to carcinogenic Substances is recommended. 331. CARCINOGENESIS INDUCED BY TRACE CONTAMINANTS IN POTABLE WATER. K ray bill HF Div. of Cancer Cause and Prevention, NCI, Bethesda, MD Bull NY Acad Med: 54(4):413-427 1978 The following are some recognized and suspect carcinogens in US drinking water and their concentrations (in ug/liter, when given): aldrin (5.4), benzene (50), benz- o(a)pyrene (0.0002-0.002), bis(2-chloroethyl)ether (0,42), lindane, carbon tetrachloride (2.0-3.0), chlordane (0.1). chloroform (0.1-3II), 1.2-dibromoethane, dieldrin (8.0). dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethylene (DDE) (0.05), 1,4-dioxane (1.0), endrin (0.004), heptachlor, trichloroethylene, and vinyl 70 yCC 106393 chloride (10.0). Of these compounds, benzo(a)pyrene and vinyl chloride are the only recognized carcinogens. Only volatile organic compounds have been identified in US drinking water, and a much larger component of nonvolatile substances remains to be identified or quantified. In city water in the Netherlands and Germany, concentration ranges of inorganic contaminants such as arsenic, cadmium, chromium, and selenium are 1.0-8.1, 2.0-9.0, 4.5-10.0, and 3.1-6.0 ug/liter, respectively. Asbestiform materials or asbestos particles have been found in many river systems at concentrations greater than 10 ug/gallon. In a study of cancer mortality in 10 river basins in the US, nickel concentrations appeared to correspond with oral and intestinal cancer death rates, arsenic with cancer of the eye and larynx and myeloid leukemia. Beryl lium correlated with bone cancer and with mortalities from breast and uterine cancer. Lead was associated with leukemia and lymphoma and with kidney, stomach, intestinal, and ovarian cancer. The results of various epidemiological studies throughout the US are reported. The need for in vitro bioassays to augment in vivo procedures in determining carcinogenicity is stressed. (30 Refs) 332. PROSPECTS FOR A REVOLUTION IN THE METHODS OF TOXICOLOGICAL EVALUATION. De Serres FJ Natl. Inst. Environmental Health Sciences, Research Triangle Park, NC Mutal Res; 38:165-176 1976 The impact of research programs to develop efficient assay systems for mutagenic activity to screen untested environmental chemicals and to develop better methods to determine their effect on man is considered. Food and feed ad ditives in widespread use such as sodium nitrite, sodium bisulfite and other nitrofuran derivatives are mutagenic in experimental organisms. A nitrofuran derivative called AF-2, which was used widely as a food preservative in Japan for 10 yr, was found to be a potent mutagen and its use as a food preservative was banned. Most pesticides are also potent mutagens in experimental organisms, and ethylene dibromide, heptachlor. and chlordane are also carcinogenic in mice and rats. Atrazine, a herbicide used widely on most commercially grown corn, is converted to a potent mutagen. Hycanthone, a drug used to treat schistosomiasis, is a supermutagen in experimental organisms. Its long-term genetic effects on treated populations have not been evaluated adequately. The majority of commercial hair dyes available in the United States, England, and Japan are potent mutagens in various short-term tests for mutagenicity. Industrial chemicals that are mutagenic in experimental organisms and have been associated with occupational carcinogenesis include bpropriolactone, ethyleneimine, 4-aminobiphenyl, 4nitrobiphenyl, bis(chloromethyl) ether, benzidine, and vinyl chloride. Vinyl chloride has produced significant levels of chromosome damage in somatic cells of exposed workers. The primary concern over the effects of environmental mutagens on man is that exposure may produce damage in germ cells that will be transmitted to future generations. Newly developed short-term tests for mutation induction include assays for both forward and reverse mutation at specific loci and tests for inhibition of DNA repair. In the assays for mutation induction, the best correlation between carcinogenic and mutagenic activity is with Salmonella; at least 70%-75% of the carcinogens tested with this system show mutagehic activity. It is generally agreed that these short-term tests provide a mechanism for identifying potential mutagenic and carcinogenic agents and that they are most effectively used to establish priorities for testing in higher organisms. (39 refs) 333. PREDICTIVE VALUE OF CARCINOGEN'- SIS BIOASSA3S. Maltom C Inst. Oncology and Tumour Centre. Bologna. Italy Ann NY Acad Sci; 271:431-443 1976 Bioussays provide an important means of predicting the oncogenic risk of particula; occupational and environmental agents to man. In fact, the three most important occurrences of environmental and occupational tumors discovered after 1970 were directly or indirectly predicted by animal studies. The first was the adolescent clear-cell vaginal adenocarcinoma found in girls born to mothers treated during pregnancy with synthetic nonsteroid estrogen therapy. As long ago as 1938, it was demonstrated that stilbestrol had carcinogenic properties, with mammary tumors arising in mule mice treated with the compound; several years later, the same hormone was shown to produce a variety of tumors among different animal species. The second occurrence was that of pulmonary carcinoma among workers exposed to bis(chloromethyl) ether, which had previously been shown to produce sc fibrosarcomas and skin carcinomas when injected sc into rats or applied topically to mice. When the compound was inhaled by rats, it induced squamous cell carcinomas of the lung. The third example was provided by the identification of liver angiosarcomas in workers occupationally exposed to vinyl chloride (VC), after the carcinogenicity of the compound had been demonstrated in rats. Data from inhalation experiments with VC in rats, mice, and hamsters and from ingestion experiments in rats are reported in detail. Sixty Swiss mice of both sexes were treated by inhalation of VC in air at 10,000 ppm for 4 hr/duy, 5 days weekly for 30 wk. After 81 wk 55 developed pulmonary tum ors, 13 had mammary carcinomas, 8 had liver angiosarcomas, 9 had vascular tumors, and 3 had epithelial tumors of the skin; of 150 untreated mice. 8 developed pulmonary tumors and I a vascular tumor after the same length of time. Bioassays can also be used to predict the carcinogenicity of inorganic substances. Various inorganic materials were tested by sc injection of 30-mg quantities into Sprague-Dawley rats. Of 40 animals in each group, 9 developed rhabdomyosarcomas and fibrosarcomas with neochromium, 8 with chromium allumen, 26 with chromium yellow, 27 with molybdenum orange, 16 with cadmium yellow, and 1 with iron, yellow, after 125-150 wk. None of 140 control animals developed neoplasms. Of 49 male and 48 female Sprague-Dawley rats, 34 and 31 developed peritoneal mesotheliomas following the endoperitoneal injection of 25 mg of crocidolite. 334. OCCUPATIONAL LUNG CANCER. (PP. 25-51) Frank AL Mount Sinai Sch. Medicine, New York, NY Pathogenesis and Therapy of Lung Cancer. Harris CC, ed. New York and Basel, Marcel Dekker, Inc., Lung Biology in Health and Disease, Vol. 10, 762 pp., 1978. An overview is presented of factors that contribute to lung cancers associated with working areas. Major occupational respiratory carcinogens discussed are arsenic, asbestos, chloromethyl ethers, chromium, carbon compounds including coke and tar, mustard gas, nickel and radiation. Ad ditional agents are discussed with proven, suspected, or possible carcinogenic risk: beryllium, carbamates, chloroprene, fibrous glass, isopropyl oil, methylene-bis-orthochloroaniline and nitrosamines. Also discussed, in connection with lung cancer, are smelting operations, various vegetable dusts, vinyl chloride and woodworking. The review concludes with a brief consideration of directions in occupational carcinogenesis. Despite the interest of various professional and legislative groups, it is pointed out that there will still be a need for the astute clinician to note unexpected associations. (146 Refs) 71 UCC 106394 INDEX TO KEY WORDS IN TITLE 251 m 294 is 3? n 123 is m m 140 143 43 12? Its <s m 82 8b 81 80 183 188 J 1 m 322 179 HfIt 70 199 124 191 19 UO 41 42 40 19? 191 301 126 191 142 197 12b 13? 319 71 236 ?3b 23? 21b ? 201 213 221 328 124 243 244 319 too m 186 186 81 MlOt MU 07 CHROMOSOMAL ALIAN ENZYME MEDIA!/ MUIAGENICUY AHO CHROMOSOMAL SURVEILLANCE OF SPONTANEOUS 0 kf FORMATION 07 IMIOAZGL DERIVATIVES Of NUCLEIC TRACT/ FORMATION Of fTHENO DERIVATIVES Of MUCUfC DETERMINATION Of THiOOlGlYCOLIC HLOROACflAlOEHYOE 2CHL0R0E1HAN0L AND CHLMQACEtiC TNG Of **14C WHtVL CHLORIDE 10 PROTEINS AND NUCLEIC f 1.2 DlCHLOROfTHANE ON SALMONELLA 1YPHWUR1UM (1 MENIAL POSSIWUUU AND CUN1CAL SKMfKJfltt / ECT ON ELECTROPHILIC REACTIVITY Of THE/ METABOLIC Cl! I CHAMCWRlZAJfON Of S S THE METABOLIC Of 12 DlCHLOROfTHANE ON SALMONELLA TYPNIMUimiM I *C*r Of VINYL CHLORIDE IN THE ABSENCE Of METABOLIC NSf IDJtlCfTir OAIA fOR CHEMICALS REQUIRING METABOLIC YL CHIORIOE MONOMER (VCM) UNOEft MAMMALIAN METABOLIC QXICITY Of BC1A CHiOROfRENE(2 CHLOROeOfAPIENE lit HENOBAftSHAL / , AND VINYL BROMIDE AFTER AROCLOR 1294 PRETREATM/ SATURABLE METABOLISM AND THE AL ENZYMES (MEETING ABSTRACT / INDUCTION Of PULMONARY OF 11-OfCHLOBOETHYlENE IN THE RAT EffECIS Of SU THE MEASUREMENT Of VINYL CHLORIDE MONOMER {VCMt IN ETHYLENE DlBRONWE (N URBAN HlflCANCE / ACTIVATION MECHANISMS IN CHLORINATED IAL MUTAGENS AND POTENTIAL MUTAGENS I HAIOGENATCO N Of STOMACH CANCER IN RATS AND MICE BY HAIOGENATCO MUTAGENICITY Of HAIOGENATCO METABOLISM Of CttlQRMATEO ALMNES AMD ME1AB0L15M Of CHLORINATED RUCTURAL CORRELATIONS Of CARCINOGENIC AND MUTAGENIC UCEO BY HUMAN AND ANIMAL TISSUES / NOS (MEETING ABSTRACT)/ **I4C VINYL CHLORIDE AND TRANSFORMATION TO PROTEIN OGEN VINYL CHLORIDE AND ITS COMPARISON WITH A KNOWN VINYL BROMIDE (MEETING ABSTRACT! i S VIVO FORMATION Of I N**6 ETHEN0 ADENOSINE WITH CARCINOGENESIS (HALOGCNATEO OLEFINS VINYL AND AUYIAIING AND MUTAGENIC EffECIS Of fOR A RECOMMENDED SIANOARO OCCUPATIONAL EXPOSURE TO GENETIC ACTIVITY Of ALKYLATING AND MUTAGENIC EFFECTS Of Aim AND ENE ON SACC/ GENETIC AND TOXICOLOGICAL EffECIS Of ARCINOGENEStS iHAlOCENATEO OLEflNS VINYL AND AllYl MUTAGENICITY AND CHROMOSOMAL ABERRATIONS AS AN MUTAGENICITY OF VOLATILE CANCER ANO CONGENITAL ANOMALIES ASSOCIATED WITH URINARY AND irSSlfE CIYCOSAMINOCTVCAH PATTERNS IN RSENIC COMPARISON WITH C1 DEVELOPMENT Of HEPATIC CLINICAL AND MORPHOLOGIC FUTURES Of HEPATIC RIDE MORPHOLOGICAL DESCRIPTION Of THE 1/ HEPATIC OARERS / ORKERS 197? UPDAIE Of THE NlOSH REGlSfER < TEN CASES OF lN CASES OF REVIEW OF GLUES Of HAL0CENAUO OLEflNS PRODUCED 0Y HUMAN AND TERNA4 CHROMOSOME STUDIES UNDERTAKEN ON PERSONS AND SONS WITH AND WITHOUT VC ILLNESS ANO ON VC EXPOSED CANCER AND CONGENITAL CHLORINATED OLEflNS WITH EMPHASIS ON SYNERGISM ANO POSSIBLE ROLE FOR ANIiMUTAGENS ANTITERAIOGENS AND VERQNMfMAL HE' EVALUATION OF A POSSIBLE ROLE FOR EVALUAIIGH Of A POSSIBLE ROLE FOR ANIIMUTAGENS l fIUORIOE VINYL CHLORIDE. ANO VINYL BROMIDE AfTER ABERRATION IN PVC WORKERS ABERRATIONS AS AN ANALYTICAL TOOL FOR IN VITRO DETECTION Of MAMM ABORTIONS POWER IN ENVIRONMENTAL MONITORING ACID BASES (DMA AND RNA| BY METABOLITES Of VINYL CHLORIDE IN VIV ACID BASES IN VIVO BY METABOLITES OF VINYL CHLORIDE (MEETING AOS ACID IN URINE SPECIMENS Of VINYL CHLORIDE EXPOSED WORKERS ACID CONCEIVABLE METABOLITES Or VINYL CHLORIDE / THE MUTAGENIC ACIDS IN VITRO AND IN VIVO (MEETING ABSTRACT! / COVALENT BIND ACTIVATION BY IHE ISOLATED PERfUSEO RAT lTVER / THE MUTAGENIC l ACTIVATION MECHANISMS IN CHLORINATED ALIPHATIC COMPOUNDS EXPERT ACTIVATION OF CHLORINATED EIHYLENES DEPENDENCE OF MUTAGENIC fff ACTIVATION OF DBCP IN THE SALMONELLA TEST SYSTEM (MEETING ABSIRA ACTIVATION Of VINYL CHLORIDE IN VITRO (MEETING ABSTRACT! ACTIVATION THROUGH CONJUGATION WITH GLUTATHION IN VITRO ' THE M ACTIVATION / THE HON MUTAGENICITY ANO RECOUBINOGEN ACTIVATION EXAMPLE VINYL CHLORIDE / RESOLUTION Of DOSE RESPG ACTIVATION STUDIES IN VITRO ANO IN VIVO / EVALUATION OF IHE GC ACUTE ANO SUBACUTE TOXlCiTr 1T ACUTE HEPATIC INJURY BY VINYL CHLORIDE IN RAIS PRETREATED WITH P ACUTE NEPATOTOXiClir Of ETHYLENE VINYL FLUORIDE VINYL CHLORIDE ACUTE TDXICIir Of I I DICHLOROETHVLENE ACUTE TRICHLOROETHYLENE HEPATOTOKICITV ANO INDUCTION OF MlCROSOM ADENOMAS M STRAIN A MICE BY SUBSTITUTED ORGANOHALIOES AGE. AND FASTING / ORAL TOXICITY AIR / A PRACTICAL METHOD FOR AIR ALIPHATIC COMPOUNDS EXPERIMENTAL POSSIBILITIES AND CLINICAL SIG ALIPHATIC DERIVATIVES ' 1NDU5IA ALIPHATIC FUMIGANTS / INDUCTlQ ALKANES ANO THEIR DERIVATIVES ALKANES AS RELATED TO TOXICITY ALKEMES AND ALKANES AS RELATED TO TOXICITY ALKYL HALIDES (PP 163 1711 ' SI ALKYLATING AND MUTAGENIC METABOLITES Of HALOGEHAffD OLEFINS PROD ALKYLATING AND MUTAGENIC EFFECTS Of ALLYL ANO AUVLOGENIC CGMPOU ALKYLATING METABOLITES IN VITRO / LIVER MICROSOMAL UPTAKE OF ALKYLATING MUTAGEN (PP 90S 919} / IHE MUTAGENICITY Of IHE CAR ALKYLATION Of DMA AND RNA BY METABOLITES OF VINYL CHLORIDE AND ALKYLATION Qf DNA AND PROTEINS IN MICE EXPOSED TO VINYL CHLORIDE ALKYLATION OF RNA BY VINYL CHLORIDE METABOLITES IN VITRO AND IN ALLYL ANALOGS ANO EPOXIDES) (PP 8 21! / STRUCTURAL PARAMETERS ALLYL AND AUVLOGENIC COMPOUNDS (MEETING ABSTRACT! ALLYL CHLORIDE CRITERIA AUYL CHLORIDE AimOGENlC COMPOUNDS (MEETING ABSTRACT! ALPHA BENZENE HEXACHL0R1DE OIBUTtl PHTHALATE AND TRICHLOROEIHYL ANALOGS ANO EPOXIDES! |PP 8 21! / STRUCIURAl PARAMETERS ASSOC ANALYTICAL TOOL FOR IN VITRO DETECTION Of MAMMALIAN ENZYMf-MEtHA ANESTHETICS (MEETING ABSTRACT l ANESTHETICS ANGIOSARCOMA AND OTHER VINYL CHIORIOE EXPOSURE-ASSOCIATED LIVER ANGIOSARCOMA IN MAN INDUCED BY VINYL CHLORIDE. iHORQIfttt! AND A ANGIOSARCOMA IN VtNYl CHLORIDE WORKERS ANGIOSARCOMA IN WORKERS fOllOWINC CHRONIC EXPOSURE ?0 WNtt CHIO ANGIOSARCOMA OF THE LIVER AN EPIDEMIOLOGIC SURVEY ANGIOSARCOMA Of THE LIVER IN VINYL CHLORIDE^ POLYVINYL CHLORIDE W ANGIOSARCOMA Of THE LIVER (N VINYL CHLORIDE/POLYVINYL CHIORIOE W ANGIOSARCOMA OF THE LIVER IN SHAWIHICAN QUEBEC ANGIOSARCOMA Of THE LIVER IN VINYL CHLORIDE WORKERS IN CANADA ANIMAL STUDIES (MEETING ABSTBACTl ANIMAL TISSUES ALKYLATING AND MUTAGENIC METAB ANIMALS WITH VC ILLNESS (MEETING ABSTRACT! l> ANIMALS / MUTAGENICITY OF VINYL CHLORIDE EXTERNAL CHROMOSOME 5 ANOMALIES ASSOCIATED WITH ANESTHETICS ANTAGONISM DAMAGE TO HEPATIC CELLULAR MEMBRANES B> AHflCARCIHOGEhS IN REDUCING ENVIRONMENT! HEAL IH HAZARDS EVAL ANTlMUIAGENS ANTITERAIOGENS AND ANTICARCtNOGENS !N REDUCING ft* ANTITERAIOGENS AND AATICARCIHOGtttS !N REDUCING ENVIRONMENT*' H( AROClOR 129* PRETREATMENI ACUTE HlPATOTOAlCtlY Of ETHYLENE \ 236 A IN MAN INDUCED BY VIHYl CHIORIOE IHORDIRAST AND ARSENIC COMPARISON WITH CASES Of UNKNOWN ETIOLOGY , DIVELOPMEN 139 7 HE PREDICTIVE VALUE Of TISSUE MEDIATED MUTAGENICITY ASSAYS 10 ASSESS IHE CARCINOGENIC RISK Of CHEMICALS (PP *67 4 MENT OF SOME POTENTIALLY HAZARDOUS MATERIALS IN THE ATMOSPHERE Of RUBBER FACTORIES , MEASURE 75 HLOROETHYIENE RELATIONSHIP Of METABOLITE LEVELS (0 ATMOSPHERIC CONCENTRATIONS PRELIMINARY COMMUNICATION / TRIC 25 STER TOTAL RADIOACTIVITY. N0NV0LAI1L/ THREE STEP AUTORADIOGRAPHY Of ORGANIC SOLVENTS ANO PLASTIC MONOMERS ID REG! 192 CTl (PP 78) MALIGNANT TRANSFORMATION Of A BABY HAMSTER LUNG CELL LINE BY 2 CHLOROBUTADHNt (MELIING ABSTHA 39 fURMAIION Of IMIOAZOL DERIVATIVES OF NUCLEIC ACID OASES (ONA ANO RNA) BY METABOLITES Of VINYL CHlORifll IN VIVO ANO 37 FORMATION Of ETHENO DERIVATIVES OF NUCLEIC ACID BASES IN VIVO BY METABOLITES OF VINYL CHLORIDE (MEETING ABSTRACT 128323 VINYL CHLORIDE AND VINYL BENZENE (STYRENE) METABOLISM MUTAGENICITY AND CARCINOGENICITY OF PETROL IN DROSOPHILA MELAN0GA5TER I EFFECTS Of BENZENE ANO I 2 DICHLOROEIHAHt ' MUIAGIMC EFFECTS 34 NTAl FATE OF THREE CARCINOGENS BENFOtAlPHAlPYRENE BENZIDINE ANO VINYL CHIORIOE EVALUATED IN LABORATORY MODEL ECOS 31 (MEftlNG ABSTRACT DISTRIBUTION Of METABOLITES Of BNZO(A)PYRNE IN THE ISOLATED PERFUSED RABBI! LONG PREPARATION 34 ABO' THE ENVIRONMENTAL FATE Of THREE CARCINOGENS BENZOLALPHAjPYRLNE 8EN2IDINE AND VINYL CHLORIDE EVALUATED IN L 149 ALUAH THE TERATOGENIC ANO EMBRYOTOKIC POTENTIAL Of BETA CHIOROPREKE (2 CHlOSOflUIAOlCHE I 3) INHALATION STUDIES ( 82 CHY TOXICITY Of SEIA CHLOROPRENC'2 CHLOROBUIADIENE I 3l ACUTE AND SUBACUTE TOW 64 MACRGMOiCCULAR BINDING AND METABOLISM OF THE CARCINOGEN 1 2 PIBROMGETHME 661? HEPATIC MACROMOLECULAR BINDING FOLLOWING EXPOSURE TO VINYL CHLORIDE PROPOSED MECHANISM OF ACTION AND DISTRIBUTION AND BINDING Of **14C RADIOACTIVITY FOLLOWING INHALATION EXPOSURE IN 33 TUENTS IN VIVO AND IN VITRO/ IRREVERSIBLE BINDING OF "I4C UBEIICD TRICHLOROETHYLENE TO MICE LIVER CONST! 18 VIISO AND IN VIVO (MEETING ABSTRACT)/ COVALENT BINDING OF **140 VINYL CHLORIDE TO PROTEINS ANO NUUUC AGIOS IN 32 IRREVERSIBLE BINDING Of CHLORINATED (THYLCffES TO MACROMOLECULES 56 OLECUUS 'MEETING . M1CROSOME DEPENDENT COVAIENT BINDING OF THE CARCINOGEN TRICHLOROETHYLENE TO CUIULAR KACROM 5? L PROTEINS ANO TO EXOGENOUS DMA IN VlTR; COVALENT BINDING OF THE CARCINOGEN MUCH' OROETHYlfNE TO HEPATIC MlCftOSOUA 63 TlTUCNIS Of FORESTOMACH AND LIVER {MEETING A8STR/ BINDING Of THE CARCINOGEN ETHYLENE DtSflOMlOE TO CHROMOSOMAL CONS 287 VINYL CHLORIDE A CASE FOR THE USE OF LABORATORY BIOASSAY IN THE REGULATORY CONTROL PROCEDURE (PP 1797 1805> 174 MEETING ABSTRACT) / 170 IY ; BIOASSAY OF I I 1 IRICHLOROEIHANE fOR POSSIBLE CARCINOGENICITY ( BIOASSAY OF 11.22 UTRACHtOROElHANE FOR POSSIBLE CARCINOGENIC! 169 REPORT ON BIOASSAY Of IT OICHIOROEIHANE FUR POSSIBLE CARCINOGENICITY 168 REPORT ON CARCINOGENESIS BIOASSAY Of I 2 OICHIOROEIHANE lEDCi 180 AS NO 1836 79 5 BIOASSAY Of DI8RDMOCHIOROPROPANE FOR POSSIBLE CARCINOGENICITY C 17? 5 331 CARCINOGENICITY BIOASSAYS OF VINrilOENE CHLORIDE RESEARCH PLAN AND EARLY RESULT PREDICTIVE VALUE Of CARCINOGENESIS BIOASSAYS 93 HLORIDE DUST IN RAT LUNG/ SOME BIOCHEMICAL ANO HISIOPAIHOLOGlCAl CHANCES INDUCED BY PQLYVINrl C 99 I I IRICH10R0E1HANE ANO TRICHLOROETHYLENE ON RAF/ BIOCHEMICAL ANO TOXICOLOGICAL EFFECTS Of COMBINED EXPOSURE 10 1 39 RAIS IHE CHEMISTRY AND BIOGENESIS Of THE S CONTAINING METABOLITES Or VINYL CHLORIDE 1H 86 BIOLOGICAL EFFECTS Of VINYL CHLORIDE AH EXPERIMENTAL STUDY 288 KS THE POSSIBLE USE Of A BIOLOGICAL EXPOSURE TEST TO DETERMINE HEALTH STANDARDS (OR WORSE 27 THE BIOLOGICAL FATE Of VlNYUDENE CHLORIDE IN RAIS 295 ASSOCIATION OF 6KIREFRACTORIE5 IN DRINKING WATER ANO BODY BURDEN IN PfOPIE 164 SI METHOD DETERMINATION OF THE BLASTOMOGENIC ACTIVITY Of SOME CHEMICAL SUOSIANHS fit A RAPiD (E 265 0PLAST1C AND C0UAGEN1ZJNG CHANCES IN THE LIVER AND BLOOD CLOTTING IN PVC WORKERS PfiiM 24 VC METABOLIC APPROACH 10 INDUSTRIAL POISONING BLOOD KINETICS AND DISTRIBUTION Of **I4C VJNYlCH'QftlDf MONOMER > 258 RS INVOLVED IN (HE MAH AN EPIDEMIOLOGIC STUDY Of BLOOD SCREENING TESTS ANO ILLNESS HISTORIES AMONG CHIMJUl WOfi*E 295 SSOCIAIION Dr BIOREFAACTORIES IN DRINKING WATER AND BODY BURDEN IN PEOPLE A 157 ONCOGENIC RESPONSE Of RAT SKIN LUNGS. AND BONES TO VINYL CHLORIDI 153 ANT TRANSFORMATION Of CELLS Of WHOLE EMBRYOS FETAL BRAIN AND NEWBORN LUNG OF HAMSTER (MEETING ABSTRACT Ur /Ufi 99 CHLOROLTHANE AND TRICHLOROETHYLENE ON RAT LIVER ANO BRAIN , BIOCHEMICAL ANO TOXICOLOGICAL EFFfCIS OF COMBINED EXPOS 214 R IN THE MANUFACTURE Of POLYVINYL CHLORIDE IN GREAT BRITAIN MORTALITY EXPERIENCE Of WORKERS UPOSEO 10 VINYL CmO 41 ANQ RNA BY METABOllTlES Of VINYL CHLORIDE AND VINYL BROMIDE (MEETING ABSTRACT) AIMUTUJN Of DN* 81 ETHYLENE VINYL FLUORIDE VINYL CHIORIOE ANO VINYL BROMIDE AfTER AROCLOR 125* PREIREATVENT ACUTE HlPAJOIGHClTY 312 VINYL HALIDES CARCINOGENICITY VINYL BROMIDE VINYL CHIORIOE AND VINYDOENE CHLORIDE 178 Si HYDROXYME IHY1 )PHOSPHONIUM CHLORIDE AND POLYVINYL BROMIDE MOUSE SKIN CARONOCLfUCfiY TESTS Of THE FIAME RllABOA 10 A HIGHLY STEREOSELECTIVE SYNTHESIS OF VINYL BROMIDES ANO CHLORIDES VIA OlSUBSKTUIfO VINYLSlLANES 91 PATlC HON PROTEIN SUlFHYPRYL CONTENT AND EFFECIS ON BROMOSULPHALEIH |8SPj CLEARANCE IN RATS VINri CHLORIDE INDUCE 91 SUlfHYDRyl CONTENT AND EFFECTS ON BROMOSUlPHAlEIH ( BSP} CLEARANCE IN RATS VINYL CHLORIDE INDUCED DEPRESSION Of 221 I0SA8COMA OF IHE UVER IN VINYL CHLORIDE WORKERS IN CANADA TEN CASES OF ARC 309 AN OVERVIEW OF THE VINYL CHLORIDE HAZARD IN CANADA 240 EXAMINATION BY IN VIVO MICROSCOPY CAPILLARY ABNORMALITIES IN POIYVINTL CHLORlDt PRODUCTION WORKERS 64 MACROMOLECULAR BINDING AND METABOLISM OF THE CARCINOGEN 1 2 DFBROMOEIHANE RACTION Of POTENTIAL ACTIVATED INTERMEDIATES Of THE CARCINOGEN ETHYLENE OlBROMiOE WITH PROTEIN ANO DNA IN viIRO (MEE 6s (STOMACH AND LIVER 'MEETING ABSTR BINDING Of THE CARCINOGEN ETHYtfNE OlBROMiOE TO CHROMOSOMAL COHSTiIUTNIS Of fOfi 326 ENVIRONMENT*! MUIACENS CARCINOGENS SESSION II CARCINOGEN SCREENING OBSTACLES AND OPHQhS IN VITRO TESTING OF 59 COVAIEHI INTERACTION Of METABOLITES Qf THE CARCINOGEN TRICHLOROETHYLENE IN AAf HEPATIC MiCROSOMES no5? 10 EXOGENOUS SNA IN VITft COVALENT BINDING Of THE CARCINOGEN TRlCHEOROEIHYLCNE TO HEPATIC MICROSOMAL PROTEINS AND TING MUTAGEN (PP 509 THE MUIACENICITY OF IHE CARCINOGEN VINYL CHLORIDE ANO ITS COMPARISON WIIH A KNOWN ALA TLA 96 ING MOOSOME DEPENDENT COVALENT BINDING Of THE CARCINOGEN TRICHLOROETHYLENE TO CELLULAR MACROMOiEUUES (MEET 218219 CASE STUDY OF VINYL CHLORIDE AS A CARCINOGEN THE CARCINOGENIC PROPERTIES OF VINYL CHLORIDE 296 HYl CHIORIOE CARCINOGENIC MUTAGENIC AND TERATOGENIC BISKS AS5QC1AIE0 WITH VI UCC 106395 UCC 106396 m ji '74 .JS n .5?1i :)6 M 06 62 '56 Si S6 `A i42 .16 148 ;IB IG1 5?9 >44 1// :4* 161 ?48 393 146 m ns 303 m 49 ? 320 3?? 197 1)9 10? J5 50 i/6 101 120 10 j 33 JO US 199 32 196 340 100 J?4 II? I?] 113 123 9 113 90 123 I 113 M? 311 224 363 225 223 226 5 6 251 124 24/ CARCINOGENS IN ORINKING MATER CARCINOGENS OCCURRENCE. FORMATION ANO ANALYSIS 'PP 1943 1949 CASE STUDY 3 VINYL CHLORIDE BEST AYAH ABLE TECHNOLOGY CASE STUOV OF VINYL CHLORIDE AS A CARCINOGEN It* CASES Of ANGIOSARCOMA Of THE LIVER IN SmAWINFGAN QUEBEC CANADA TEN CASES OF ANGIOSARCOMA OT THE LIVER >H *Jfm CHlORIOE WORKERS IN RESULTS Of A STUDY Of U CASES Of LONG IERM EXPOSURE ID VINYL CHLORIDE CHlOfrOf SH0RQ1RA5T AND ARSE NEC COMPARISON WtlH CASES OF UNKNOWN ETIOLOGY DEVELOPMENT OF HEPATIC ANGIOSARCOMA CEGA1 DECISIONS AND OPINIONS 'N POUOHGH CASES .INTL CHLORIDE dominant lethal S1U0IES IN male CO I MICE MAllCWNf (MNSfORMAIlON Of A BABY HAMSTEA LUNG CEU LINE BY 2 CHLOROBUTAOIENE lMEETINC ABSIRACTi Fr 7gj MlCROSOME MEDIATED MUlAGENESlS Of A CHINESE HAMSTER CEU LINE BY VARIOUS CHEMICALS iMETJJNC ABSTRACT) MULING A IN VlIRO MALIGNANT TRAJlSfORMAllOH Of CELLS OT WHOLE EMBRYOS FEIAl BRAIN ANO NEWBORN LUNG Of HAMSTER EO IN JlRO FOR 2 t 2 YEARS HAMSTER CtUS UNTREATED AND TREATED WITH CHEMICAL CARCINOGENS MAINTAIN S! BiNOlNG OfiHt CARCINOGEN HtlCVUOROETHIlEHE 10 CELlUltf MACR0M01ECU115 (MEETING ABSTRACT! MlCROSOME OIPENDE GISM AND ANTAGONISM DAMAGE 10 HEPATIC CELLULAR MEMBRANES BY CHLORINATED OLEFINS WITH EMPHASIS ON SYNER A PHIHALAIE AKO TftlCHl0ROETHYlEftE ON SACCHAftOMYCES CEREVISIAE STRAIN AV1B5T4C FOR REVERSION STUDIES (MEETING ABSTR TOCINiC AND (MBRYOlOXlC EffECT Of VINYL CHLORIDE IN CFV RATS STUDY OF THE TERR. * Uufi.NC AU imstos Of PREGNANCY ON THE FETUSES Of CfY MIS . IFFEC1S Of VINYL CHLORIDE EXPOSURE ALONE ANO IN COMB PP ]?? l9t OCCUPATIONAL CHEMICAL CARCINOGENESIS NEW FACTS PRIORITIES AND PERSPECTIVES EVALUATING GENEtIC AND EPIGENETIC CONTRIBUTIONS 10 CHEMICAL CARCINOGENESIS (MEETING ABSTRACT) AN EXPERIMENTAL AP MUlACENIClTY TESTS IN CHEMICAL CARCINOGENESIS (PP 229 240) HAMSTER CEUS UNTREATED ano TREATED RUIN CHEMICAL CARCINOGENS. MAINTAINED IN VlIRO FOR ? 1? YEARS POrfNTlAt ME(AfiOtlTES :Mff? CARBONS CARCINOGENICITY AW CHEMICAL REACTION WITH GUANINE Of l CKU3ROPROPENE ANO TWO Of ITS CHEMICAL STRUCTURE REACTIVITY AND CARCINOGENICITY Of HALOHTDRO 0ETLRMINA1IQN Of THE BLASTOMOGENIC ACTIVITY OF SOME CHEMICAL SUBSTANCES BY A RAPID 1 tST METHOD f BtOOD SCREENING TESTS AND ILLNESS HISTORIES AMONG CHEMICAL WOMENS INVOLVED IN THE MANUFACTURE OF POLYVINYL CHLORI INQCENICITY AND TUMOR ENHANCING ACTIVlEY IN VARIOUS CHEMICALS (MEETING ABSTRACT) |PP 130 131) / STRUCTURAL PROGN I AGENESIS Of A CHINESE HAMSTER CELL LINE IY VARIOUS CHEMICALS IMEETJNG ABSTRACT) / MlCROSOME MEDIATED MU NtOPtASIlC OCCUPATIONAL INJURIES OUE TQ INDUSTRIAL CHEMICALS (MEflING ABSTRACT) , MEDICAL SURVEILLANCE SYSTEM FOR MUTAGENIC ACTIVITY OF CHEMICALS IDENTIFIED IN DRINKING WATER (MEE1INC ABS1RACT) l HALOGENATEO INDUSTRIAL CARCINOGENIC AND MUTAGENIC CHEMICALS II HAlOGEHAlEO SATURATED HYDROCARBONS ' POTENTIA EFFECTS Of ENVIRONMENTAL CHEMICALS ON THE GfHfllC REGULATION Of MICROSOMAL ENZYME SYS1FMS RESOLUMQN OF DOSE RESPONSE TOUCHY DATA FOR chemicals requiring metabolic activation example vinyl chiorio ICINALLY UPOSED 10 VINH CHLORIDE MONOMER AND OTHER CHEMICALS / CHROMOSOMAL DAMAGE IN MEN OCCUPAT GENETIC EFFECTS ASSQCkAfEO WITH INDUS TRIG. CHEMICALS (PP 100113) ,'NEJlCS IN THE ASSESSMENT Of CARCINOGENIC HAZARD OF CHEMICALS (PP I87Z03) / THE RELEVANCE OF DOSE DEPENDENT PHAR iNOGENIClTY AND TUMOR ENHANCING ACTIVITY IN VARIOUS CHEMICALS (PP 20/1 2094) ; STRUCTURAL PROGNOSLlCAIION Of CARC AGENICIIY ASSAYS IQ ASSESS THE CARCINOGENIC MlSH Of CHEMICALS (PP 467 491) / THE PREDICTIVE VALUE Of TISSUE MEDIA < hAlOGIMIEQ INDUSTRIAL CARCINOGENIC ANO MUTAGENIC CHEMICALS I HALOGENATEO UN SATURATED HYDROCARBONS / POIENTIA L Che URiDf IN RAIS THE ChEMISIRY AND BIOGENESIS OF THE S CONTAINING METABOLITES OF VlNY UF <>nn CHLORIDE MONOMER CH LORO ETHYLENE OXlOE WO CHLORACETAIOEHVOE ON PNA SYNTHESIS IN REGENERATING RAT LIVER ROPri iP.ioSPHAJE IETRAiuSlHYORQIYMETHYUPHOSPHONFUM Chloride ano polyvinyl bromide GENETIC AC 11VI rr Of ALLYL CHLORIDE mouse shin carcinogenicity te Rf COMMENDED SEWDARD OCCUPAllONAL EXPOSURE TO ALLYL CHLORIDE E MUTAGENIC PROPERTIES Of VINYL CHLORIDE ANO METHYL chloride , CRITERIA FOR A / a comparison or lh HLY STEREOSELECTIVE SYNTHESIS OF VINYL BROMIDES AND CHlOAlOfS VIA DISUBSEJTUTEO VlNVlSHANES / A HlG clinical significance actjvaeion mechanisms in CHLORINATED ALIPHATIC CQMPOUNOS EXPERIMENTAL POSSIBILITIES ANO MEIABOLISM OF CHLORINATED ALKENES AND ALKANES AS RELATED TO TOXICITY UFAGENJC ACTIVITY OF HAlOGENATED COMPOUNDS FOUND IN CHLORINATED ORlNJflNC WATER / THE M CARCINOGENIC POTENTIAL OF CHLORINATED ETHYLENES TENTATIVE MOLECULAR RULES (PP 1711751 IRREVERSIBLE BINDING Of CHLORINATED ETHYLENES TO UACROMOUCUICS HE OXIRANE FORMATION Of TRICHLOROETHYLENE ANO OTHER Chlorinated ethvlenes (hefting abstract) / molecular aspects to PHILIC REACTIVITY Of 1HE/ METABOLIC ACTIVATION OF chlorinated ethylemes dependence of mutagenic effect oh electro DAMAGE TO HEPATIC CELLULAR MEMBRANES BY CHLORINATED OLEFINS WITH EMPHASIS ON SYNERGISM ANO ANTAGONISM RECENT FINDINGS ON THE CARCINOGENICITY OF CHLORINATED OLEFINS HUlAGENIClTY VIA THE METABOLITES CHLOROOHRANE AND IVABLE2 THE MUTAGENICITY Of CHUJROETHYlENE OXIDE ETHANE lETHVLENE DICHLORlOEl. CH/ MUTAGENICITY Of lENE OXIDE CHLOROACCTALOE HYDE 2 CHLQROETHANQL ANO CHIOROACETAIOEHVOC MONOMER HVDRAFf / VINYL CHLORIOE CHLQftOACETAlOEHVQE. 2 CHLQROETHAMQl AND CKLOROACEHC AClO COHCC CHLOROACETALDEHVDE A POSSIBLE METABOLIC PRODUCT Of 12DICHLORO CHIGROACLTLC AClO CONCEIVABLE METABOLITES OF VINYL CHLORIOE SYNTHESIS ANO REACTIONS Of CHLOIOAIMNE EPOXIDES ROOUCT OF I ? OICHLOROETHANE (ETHYLENE OlCHLOfllOEl CHIOROCTHANOL lETHYlCKE CHLQROHYDRIK) VINYL CHLORIDE ANO CYCLOP ECfNfJUJMI/ THE EFFECT Of VINT1 CHLORIDE MONOMER CHLORQEIHYLENE OXIDE AND CHL0RACE1ALDEHVDE OK DNA SYNTHESIS IN R lOROACEllC ACID. CONCEIVABLE; THE MUTAGENICITY OF CtHOROETHYlENE OXIDE. CHIOROACETALOEHYDE, Z CHLOROETHANOL ANO CH VAPOR INFRARED SPECTAGPH010METRTC IDENTIFICATION Of HANE (ETHYLENE BICHLORIDE). CHUMGETHMtOL (ETHYLENE VINYL CHLORIDE MUTAGENICITY VFA THE METABOLITES TS CAfiClNOGfNlCJTVV IUNG CANCER INCIDENCE AMONG EXPERIMENTAL STUDY Of THE CARCINOGENICITY OF CHLOROFORM HALOTHANE MflHOXYFLURANE. TElftACHLOROiTHYLENE WO CHLOROHYDRtNI VINYL CHLORIOE ANO CYCLOPHOSPHAMIDE (MEETING SST CHLOROOX1RANE AND CHIOROACETALOEHYDE MONOMER HYDRATE CHIQROPREKE (2 CHLQRO 13 BUTA1HENE) WHAT IS THE EVIOENCE TOR 1 CHLOROPREKL HANDLING WORKERS CHLQROPREfti M0RTALJ1Y OF WORKERS UPOSEO TO CHLOROPRENi CANCER RISK AMONG WORKERS EXPOSED TO P 204 217)/ HALIDES / N URINE/ GAS GAS LIQUID HIGH RATE Of HON or MAMMAtlW ENZYME MEDIA!/ MUTAGENICITY ANO CHLOROPRENE CHLOROPRENE OBSERVATIONS OF CARCINOGENESIS AND MUTAGENESIS (P CHROMATOGRAPHIC ANALYSIS Of HAlOGENATED HYDROCARBONS IN SILICON! CHROMATOGRAPHIC DELERULNAFION OF TRICHLOROETHYLENE METABOLITES I CHROMOSOMAL ABERRATION IN PVC WORKERS CHROMOSOMAL ABERRATIONS AS AN ANALYTICAL TOOL FOR IN VlIRO DETEC CHROMOSOMAL ANALYSES IN VINYL CHLORIDE EXPOSED WORKERS 63 246 244 J5Z 243 23/ ?8 161 234 232 91 234 133 253 265 209 227 264 149 265 271 120 no 236 103 It? 191 134 136 114 133 104 330 319 127 2% 331 144 270 282 208 115 199 18 57 56 49 113 95 46 45 24? 250 245 246 249 143 300 144 95 97 299 74 9? 37 39 322 141 204 31? 1507 63 62 299 ID? BINDING OF 1H LARCINQCEN EIHYLENf OlBROMIDE 10 Of MONOMER AND OTHER CHEMICALS N VC EX MUTAGENICITY OF VINYL CHLORIDE EXlERNAl NESS (MEEIING ABSIfiACI; EXTERNAL THE l KtPAllC ANGIOSARCOMA IN WORKERS FOLLOWING IN RATS RESULTS Of A TWO YEAR ULTRASTRUCTURE OF LIVER DAMAGE IN MORPHOLOGICAL CHANGES OF 1HC LIVER AFTER HYDRYL CONTENT ANO EFFECTS ON BROMOSULPHAlEJN (BSP) VL CHLORIDE WORKERS ALIPHATIC COMPOUNDS EXPERIMENTAL POSSIBILITIES AND DAMAGE 6Y TRICHLOROETHYLENE AN EPIDEMIOLOGICAL AND tC ANO COLLAGEN!/INC CHANGES IN THE LIVER AND BLOOD MORTALITY EXPERIENCE OF A A KERS PRENEOPLASTIC AND EFFECTS OF VINYL CHLORIDE EXPOSURE ALONE ANO IN ONCOGENIC AND MUTAGENIC RISKS IN HYL CHLORIOE : A TAGENJClTY OF THE CARCINOGEN VINYL CHLORIOE AND ITS INDUCED BY VINYL CHLORIOE. 1HOROTRAST AND ARSENIC (MEETING ABSTRACT) MUEAGENICITY OF 1NOUS1RJAL AGENICIIY Of VINYL CHLORIDE METABOLITES AND RELATED TING ANO MUTAGENfC EffECTS OF ALLYL ANO AUYLOGENlC BSTRACl!UUlAGENtCITY Of INDUSTRIAL THE MUTAGENIC AC 11VI IT OF HALOGENATEO HIU1Y AND HE1ABDLJSM Of VINYL CHLORIDE AND RELATED ACnVAIfUN MECHANISMS IN CHLORINATED ALIPHATIC S (MEETING ABSTRACT) MUTAGENICITY OF INDUSTRIAL OCCUPATIONAL CANCER DISCUSSED A1 NEW YORK CANCER AND ANE ON SALMONELLA TYPHIMURJUM I ACTIVATION THROUGH THE RISK Of OBCP)i CARCINOGENESIS INDUCED BY TRACE THE INFLUENCE Of EXPERIENCE IN INDUSTRIAL EXPOSURE NCE IN 594 EMPLOYEE VINYL CHLORIDE EXPOSURE (N A INDUCTION Of GENE MU1ATJONS AND GENE 163 171)/ STRUCTURAL ACIDS IN VITRO AND IN VIVO IMLEIING ABSTRACT); MICRQSOMAL PROTEINS AND TO EXOGENOUS DNA \H VJTR/ AR MACROtfOlLCDLES (MEETING . MlCROSOME DEPENDENT THVLENE IN RAT HEPATIC MlCROSOMES/ ETHANOL (ETHYLENE CHlOROHYDRIN) VINYL CHLORIDE AND TRICHLOROETHYLENE INDUCED DEACTIVATION Of ION OF THE HEME OF HIGHLY PURIFIED LIVER MICROSOMAL VINYL CHLORIOE MEDIATED UION OF VINYL CHLORIDE WITH RAL HEPATIC MICROSOMAL INDUSTRIAL MONIIORINC A EFFECTS OF VINYL CHLORIOE IN MAN A 0 VINYL CHLORIDE / Q VINYL CHLORIDE (MEEIING ABSTRACT)/ VINYL CHLORIDE CHARACTERIZATION Of S9 ACTIVATION Of DIBROMOCHLOROPROPANE ( ON THE MUTAGENIC AClIVlTY OF DIBROMOCHLOROPROPANE ( N VIVO/ LRICHIOROEIHYIENE INDUCED 11 ION (MEETINGABSTRACT TRICHLOROETHYLENE INDUCED LEGAL LUA1 ION OF EXPOSURE TO TRICHLOROETHYLENE DURING THE TlON OF LIVER ENDOPLASMIC RETICULUM AND GLUTATHIONE L CHLORIDE (MEETING ABSTRACT/ FORMATION Of ETHENO F VINYL CHLORIOE IN VIVO A/ FORMATION OF IMIOAZQL NS AND POTENTIAL MUTAGENS I HALOGENATEO ALIPHATIC MUTAGENICITY OF HALOGENATEO ALKANES AND THEIR 25 4261/ PRIMARY LIVER CANCER REVIEW OfSELECTED LI1ERATORC ON ETHYLENE INHALATION OF ETHYLENE EfHYLLNE MEETING ABSTR/ BINDING Of THE CARCINOGEN FIHYLfNE ACTIVATED INIERMEOIA1ES Of THE CARCINOGEN ETHYLENE CARCINOGENIC RISK ASSESSMENT ETHYLENE REPAIR OF AAf LIVER DNA IN VIVO DAMAGED BY ETHYLENE CHROMOSOMAL CONSTITUENTS OF FDRESLOMACH ANO LIVER iMEElLNG ABSTR CHROMOSOMAL DAMAGE IN MIN OCtUPAIIONAUY EXPOSED 10 VINYL CHIQRI CHROMOSOME STUDIES ON PERSONS WITH AND WITHOUT VC ILLNESS AND 0 CHROMOSOME STUDIES OF TRICHLOROETHYLENE WORKERS CHROMOSOME STUDIES UNDERIAftfN ON PERSONS ANO ANlMAiS WITH VC III CHRONIC EXPOSURE TO VINYL CHLORIDE MORPHOLOGICAL OESCRlPHON OF CHRONIC TOXICITY AND REPRODUCTION STUDIES OF HUACHLOROBUTAOIENE CHRONIC TOXtClH STUDY WIIH HLXACH(QROBUTADlEN RATS CHRONIC VINYL CHLORIDE INIOXICAnON CHRONIC VINYL CHLORIDE INIOXJCALION CLEARANCE IN RATS VINYL CHLORIDE INDUCED DEPRESSION OF HERAT I CLINICAL ANO MORPHOLOGIC FEATURES Of HEPAIlC ANGIOSARCOMA IN VlN CLINICAL SIGNIFICANCE ACTIVATION MECHANISMS IN CHLORINATED CUHICAL EXPERIMENIAl STUDY HEALTH CLOTTING IN PVC WORXtRS PRENEOPLASI COHORT OF VINYL CHLORIDE POLYVINYL CHLORIDE WORKERS COHORT STUDY ON TRICHLOROETHYLENE EXPOSURE AND CANCER MOHIAIilf CQUAGENI21NG CHANGES IN 3HE UVLR AND BLOOD CLOIUNG IN PVC WOR COMBINATION WITH TRYPAN BLUE APPLIED SYSTEMATICALLY OURING ALL COMMUNITIES WTH POLYVINYL CHLORIDE PRODUCTION FACILITIES COMMUNITY RISK ASSESSMENT COMPARISON OF THE MUIAGENlC PROPERTIES OF VINYL CHLORIOE AND MEI COMPARISON WITH A KNOWN ALKYLATING MUTAGEN (PP 505 5)9) THE COMPARISON ttCLH CASES Of UNKNOWN ETIOLOGY . DfVELOPMfNI Of HtPA COMPOUND VINYL CHLORIOE STYRENE ANO THEIR PUSS I Bit ME I AGOUTIS COMPOUNDS (MEETING ABSTRACT) (PP 192 193) . STUDIES ON THE M COMPOUNDS (MEETING ABSTRACT) AIKTLA COMPOUNDS EVALUATED BY MEANS OF YEAS) GENETIC SYSTEMS (MEETING A COMPOUNDS FOUND IN CHLORINATED DRINKING WATER COMPOUNDS MUTAGE COMPOUNDS EXPERIMENTAL POSSIBILITIES ANO CLINICAL SIGNIFICANCE COMPOUNDS VINYL CHLORIDE STYRtKE ANO 1HEIR POSSIBLE METABOLITE CONFERENCE CONGENITAL ANOMALIES ASSOCIATED WITH ANESTHETICS CONJUGATION WITH GLUTATHION IN VlIRO THE MUTAGENIC EFFECT Of CONSUMERS ANO PVC WORKERS TO VINYL CHLORIDE CONTAMINANTS IN POIABlE WAlffl CONTAMINANTS ON THE MUTAGENIC ACTIVITY OF OlBRQMOCHlORQPROPANL L CONTROL STATUS Of VJNYl CHLORIOE CONTROL CONTROLLED INDUSTRIAL ENVIRONMENT A LONGTERM MORTALITY EXPEfllE CONVERSIONS BY VINYL CHLORIDE MEIABOUTES IN YEAST CORRELATIONS OF CARCINOGENIC ANO MUTAGENIC ALKYL HALIDES IPP COVALENT BINDING Of **l4CVfNYL CHLORIDE TO PROTEINS AND NUCLEIC COVALENT BINDING OF THE CARCINOGEN TRICHLOROETHYLENE TO HEPAIlC COVALENT BINDING OF THE CARCINOGEN 1RICHLQR0ETHYLENE 10 CEUUl COVALENT INTERACTION OF METABOLITES Of THE CARCINOGEN 1RICHL0RQE CYCLOPHOSPHAMIDE I MEETING ABSTRACT) / MUTAGENICITY Of CHLOROACE CYIOCHROME P 450 AND LOSS Of LIVER GLU1A1HIQNE IN VIVO CYTOCHROME P 450 AND THE METABOLISM OF VINYL CHLORIDE CYTOCHROME P 45Q BY A METABOLITE METABOLISM OF VINYL CHLORIDE CYTOCHROME P 450 DESTRUCTION (MEETING ABSTRACT) CYTOCHROME P 450 IN VITRO / THE INIERA CYTOGENETIC APPROACH CYTOGENETIC FOLLOWUP STUDY CYTOGENETIC INVESTIGATIONS ON LYMPHOCYTES FROM WORKERS EXPOSED I CYTOGENETIC INVESTIGATIONS ON LYMPHOCYTES FROM WORKERS EXPOSED T CYTOGENETICS OBCP IN THE SALMONELLA TEST SYSTEM (MEETING ABSTRACT DBCP) (MEETING ABSTRACT) DBCP) / 1HE INFLUENCE Of CONTAMINANTS DEACTIVATION OF CYTOCHROME P 450 AND LOSS Of LIVER GLUTATHIONE I DEACTIVATION OF LIVER ENDOPLASMIC RETICULUM ANO GLUTATHIONE GfPl DECISIONS ANO OPINIONS IN POLLUTION CASES DEGREASING OF SMALL COMPONENTS IN A MACHINERY PLANE / EVA DEPLETION (MEETING ABSTRACT) / TRICHLOROETHYLENE INDUCE0 DEACTI DERIVATIVES OF NUCLEIC AClO BASES IN VIVO BY METABOLITES OF VlNY DERIVATIVES OF NUCLEIC ACID BASES (DNA ANO RNA) BY METASOUIES 0 DERIVATIVES / INDUSTRIAL MUTAGE DERIVATIVES DETECTION IN VINYL CHLORIDE WORKERS (MEETING ABSTRACT) (PP 4 OIBRQMIDE (LOB) 0I8R0MIDE DURING GESTATION BY RATS ANO MICE DlfiROMIDE IN URBAN AIR DIBROMIDE TO CHROMOSOMAL CONSTITUENTS OF FQRES10MACH ANO LIVER ( DI8R0MIDE WITH PROTEIN AND DNA IN VlIRO (MEfllNG ABSTRAC1) OEBROMIOE DIBROMIDE UCC 106397 316 268 ESQ 144 300 J42 113 m 65 239 308 21? 260 304 267 284 28 12 1ST 42 41 39 6$ 62 97 102 36 90 J37 77 I OS JOG 23 327 87 49 135 295 294 ^ UI 136 231 229 129 128 122 228 93 34 317 166 69 140 153 146 t*9 209 216 58 98 97 J99 193 209 321 293 34 166 254 326 238 321 ]?S % 258 216 253 207 ETHYIENE DONE DEPRESSION IN fES?EClDE APPLICATORS EXPOSED E0 75 5 / BlOASSAY OF LLlENCE OF CONTAMINANTS ON INF MUONIC ACTIVITY OF OXJCOLQGlCAL EFFECTS OF ALPHA BENZENE HtXACHlOftVDE. E MEIABOUC PRODUCT OF 1.2 DICHLQROETHANf (ETHYLENE LA ANO STHEPTOMYCES / MUTAGEN LCI1Y OF OF ACTIVATED CARCINOGENIC INTERMEDIATES OF ElHYLENf THE CONDOIDN Of 1HE SPLEEN DURING VINYL CHLORIDE AND OBSERVATIONS ON THE PROGRESS OF VINYL CHLORIDE HEPATIC APMDSCOPIC ASPECTS / LIVER VINYL CHLORIDE ASSOCIATED UVER AL MECHANISMS IH THE FATHQGEftESIS Of VlHYl CHLORIDE RCHA POLYVINVL CHLORIDE/ OCCUPATIONAL SINGLE ORAL ADMINISTRATION IN RAIS (HEE1LNG MS/ TS INHALED l 2 OI8IOMK1HANE UNMET THE INFLUENCE OF (MEETING ABSTRACT!/ ALKYIAIIQH Of ALKYLATION Of ION OF INUOA2QL DERIVATIVES OF NUCLEIC ACID USES { MERMEDTATES Of ETHYLENE OIHAUDES WITH PROTEIN AND THE CARCINOGEN ETHYLENE WBSOMiDE WITH PROIflN AND EKE TO HEPATIC MICROSOMAL PROTEINS AND TO EROGENOUS REPAIR DF RAT LIVER INTERACTIONS DF VINYL CHLORIDE WITH RAl LIVffi OMER CHLOAOETHYLENE OJCIDE ANO CKtGRACE IALDEHYDE ON THE MUTAGENICITY AND EFFECTS OF POLYVINYL CHLORIDE INGESTION ST INVt CHLORIDE OR VlNVLIDENE CHLORIDE/ A VINYL CHLORIDE HIP TO ONCOGENICITY IN RATS, C HAZARD OF CHEMICALS (PF 18/ THE RELEVANCE OF IN MICE (MEETING ABSTRACT) / SOME ASPECTS ON FIXATION CJIAMPIE VINYL CHLORIDE/ RESOLUTION OF MUTAGENIC ACTIVITY OF CHEMICALS IDENTIFIED IN ASSOCIATION OF BIORCFRACTORIES IN MV OF SCIENCES / IH RAIS OF WNYCIOENE CHLORIDE INCORPORATED IN THE IVITV OF HALOGENAHD COMPOUNDS FOUND IN CHLORINATED SOCIAIIONS Of CANCER MORTALITY WITH HUGHETHANES IN CARCINOGENS IH MUTAGENIC EFFECTS OF VINYL CHLORIDE IN THANE/ MUTAGENIC EFFECTS OF PETROL IN M PHENOBA/ MUTAGENIC EFFEC1S Of VINYL CHLORIDE ON MORTALITY AMONG LAUNDRY AND OPAlHOtOCICAl CHANCES INDUCED BY POLYVINYL CHLORIDE C AND VINYL CHLORIDE EVALUATED IN LABORATORY MODEL VIEW OF SELECTED LITERATURE ON ETHYLENE DIBROMIDE { ON CARCINOGENESIS BIOASSAY OF I 2 DICHlOROETHANE ( BENZYL IPYRIDINE INBP) OR WITH SAL MO' DETECTION OF MATEO ETHYICNES OEPENOENCE OF MUTAGENIC EFFECT ON IN VITRO MALIGNANT TRANSFORMATION OF CELLS OF WHOLE STUDY OF THE TERATOGENIC AND INHALATION STUDIES TO EVALUATE THE TERATOGENIC AND NVIRONMtNT A LONG TERM MORTttW EXPERIENCE IN 594 MORTALITY AMONG INTERACTION BETWEEN 1AICHL0R0FTHYLENE AND HEPATIC LIVEN TRICHLOROETHYLENE INDUCED DEAC1IVAII0N OF LIVER FASTED MALE RAIS WITH ? 3 EPQXVPAOPAH I 01 / CTOAAL PROGNOSTICATION OF CARCINOGENICITY AND TUMOR VINYL CHLORIDE EXPOSURE IN A CONTROLLED INDUSTRIAL ENZYME SYSTEMS. EFFECTS OF ZIDINE AND VINYL CHLORIDE EVAL0A11D IN IABO' THE NS ANIITERATOGENS ANO ANNCARCINOGENS IN AEDUCINC SURVEILLANCE OF SPONTANEOUS ABORTIONS POWER IN AECNINC 06S1ACLE5 At DPI IONS IN VITRO TESTING OF OF SPLEENS IN VINUCHUMIDE DISEASE 1 l CHEMICALS ON IKE GENETIC REGULATION OF MICROSOMAL ANALYTICAL 1Q0L TOR IN VI1R0 DETECTION OF MAMMALIAN ElHYlENE HEPATQIGXtCUV ANO INDUCTION Of MICROSOMAL ES AMONG CHEMICAL WORKERS INVOLVED IN THE MAN AN ANGIOSARCOMA 01 1HE LIVER AN HEALTH DAMAGE BY lRlCHLOROETHVlENE AN UNITED STALLS (MEElING ABSTRACT* Dl BROMIDE |PP 195 2091 DlflRQMGCHlOROfROPWlE / SPERM C DIBROMQCHLOROPROPAHE FOR POSSIBLE CARCINOGEN !CJ TV CAS NO 1936 DtBROMOCHlOROFROPAM\ (DfiCP) / 1HE INF DJBROMOCKLOROPPOPANE (DBCP) (MEETING ABSTRACT) DIBU1YI PHIHALATE AND TRICHLOROETHYLENE ON SACCHAROMYCES CEREVIS DICHLORIDE). CHLQROETHANOL (ETHYLENE CHtQROHYORINl VINYL CHIORI OICHLORVOS AND OTHER STRUCTURALLY RELATED PESTICIDES IN SALMONEL OIHAUDES WITH PROTEIN ANO DNA IN MICE AND RATS TISSUES IH VITRO DISEASE (MEETING A6S1RAC1) DISEASE (MEETING ABSTRACT) s -NEW DISCOVERIES DISEASE AMONG WORKERS AT A VINYL CHLORIDE POLYMERI2ATION PLANT DISEASE ft POLYVINYL CHLORIDE PRODUCT ION WORKERS CLINICAL AND L DISEASE DISEASE / IMMUNOLOGIC DISEASES IN CONNECTION WITH THE MANUFACTURE Of PIASIIC BOTTLES F DISPOSITION AND METABOLISM Of **I4C 1 I DICHLOROCTHYLEKE AFTER DISPOSITION OF I 2** 1* C VINYL CHLORIDE IN THE RAT OISOLFIRAM TREATMENT (MEETING ABSTRACT) ' OCCURRENCE OF HEPATOC DNA MB PROTEINS IH MICE UPOSED TO VINYL CHLORIDE OKA AND RNA BY METAOOLLTlES Of VINYL CHLORIDE ANO VINYL BROMIDE DMA AND RNA) BY METABOtllES OF VINYL CHLORIDE IN VIVO ANO IN VIT OHA IN MICE AND RATS TISSUES IN VITRO (MEETING ABSTRACT) f INTE DNA Ml VITRO (MEElING ABS1RAC1) / INTERACTION OF P01ENFIAL AClk DNA IN VITRO ' COVALENT BINDING Of THE CARCINOGEN IRICHlOROETHV ONA IN VIVO DAMAGED BY ETHYLENE DI8R0MI0E ONA IN ViVO DNA SYNTHESIS ft RECINERATING RAT LIVER / THE EFFECT OF VINYL C DNA MODtFVWC EFFECT OF HALDAIKANES DOGS DOMINANT LETHAL STUDY IN MALE RATS AFTER REPEATED EXPOSURES 10 V DOMINANT LETHAL STUDIES IN MALE CD I MICE DOSE DEPENDENT FATE OF VINYL CHLORIDE AND ITS POSSIBLE RCLA1J0NS DOSE DEPENDENT PHARMACOKINETICS IN THE ASSESSMENT OF CARCINOGEN I DOSE RESPONSE IN VINYL CHLORIDE INDUCED LIVER INJURY ANO TUMORS DOSE RESPONSE TOXICITY DATA FOR CHEMICALS REQUIRING METABOLIC AC DRINKING WATER (MEETING ABSTRACT) DRINKING WATER AMD BODY BURDEN IN PEOPLE DRINKING WATER AND HEALTH RECOMMENDATIONS OF THE NATIONAL ACADF DRINKING WATER OR ADMINISTERED BY REPEATED INHALAI ION , INTERIM DRINKING WATER THE MUTAGENIC ACT DRINKING WATER / AS DRINKING WATER DROSOPHIU MELANQGASHR (MEETING ABSUtACTl DROSOPHILA MELANOGASTER I EFFECTS OF BENZENE AND I 2 DlCHLOROl DROSOPHILA MELANOGASTER WI1H AND WJ1H0UT PAE1AEATMEN1 WI1H SODIU DRY CLEANING WORKERS (MEETING ABSTRACT! DOST IN RAT 1UNC ' SOMF BIOCHEMICAL AND HIST EC0SYS1EMS / 1HE ENVIRONMENTAL FATE OF THREE CARCINOGENS BEN70 EDB) EDCI ' RE i REPORI ELECTROPHILIC METABOLITES Of HALOGENATED OLEFINS WITH 4 (4 HtlRO ELECTROPHILIC REACTIVITY OF THE MfTABOilCALLY FORMEO EPOXIDES EMBRYOS fETAL BRAIN AND NEWBORN LUNG OF HAMSIER (MEElING AfiSTR EMBRVOfOXIC EffECl Of VINYL CHLORIDE IN CFY RATS EM6RY0TDIIC POTENTIAL OF BE1A CHLOROPRENE 12 CHLOROBUTADIFNE I 3 EMPLOYEES / VINYL CHLORIDE EXPOSURE IN A CONTROLLED INDUSTRIAL E EMPLOYEES OF FVC FABRICATORS ENDOPLASMIC RETICULUM (MEETING ABSTRACT) (PP 69 TO) ENDOPLASMIC RETICULUM TARGE1 SITE OF kUUOCARBON METABOLITES ENDOPLASMIC RETICULUM AND GLUTATHIONE DEPLETION {MEETING ABSIRAC ENHANCEMENT OF I 1 DICHLOROFTHYLENE TOXICITY BY PRETREATMENT OF ENHANCING ACTIVITY IH VARIOUS CHEMICALS (MEElING ABSTRACT) IP ENVIRONMENT A LONG TERM MORTALITY EXPERIENCE IH 594 EMPLOYEES ENVIRONMENTAL CHEMICALS ON THE GENETIC REGULATION OF MICROSOMAL ENVIRONMENTAL CONCERNS BEYOND THE WORKPLACE ENVIRONMENTAL FATE OF THREE CARCINOGENS BfN20|ALPHA>PYREHE BEN ENVIRONMENTAL HEALTH HAZARDS / EVALUATION OF A POSSIBLE SOU EO ENVIRONMENTAL MONITORING ENVIRONMENTAL MUTAGENS CARCINOGENS (PP 27 33) r 5ESSI0N ll CA ENZYME HIS10CHEMICAL HtSTOMETRICAL AND ULTRASTRUCTUHAl STUDIES ENZYME SYSTEMS EFFECTS OF ENVIRONMENT* ENZYME MEDIATED FORMATION OF REACTIVE METABOLITES MUTACENJClT ENZYMES (MUTING ABSTRACT) ACUTE TRICHlOftO EPIDEMIOLOGIC S1UBY OF BLOOD SCREENING IfS'S ANO ILLNESS HISIORl EPIOEMrOlOGIC SURVEY EPIDEMIOLOGICAL AND CLINICAL EXPERIMENTAL SiUOv EPIDEMIOLOGICAL STUDIES OF VINYL CHLORIDE HEALTH EFFECTS IN Tttl 222 VINYL CHLORIDE EPIDEMIOLOGICAL STUDIES AND PREVENTIVE MEASURES 101 AN EXPERIMENTAL APPROACH FOR EVALUATING GENETIC AND EPIGENETIC CONTRIBUTIONS TO CHEMICAL CARCINOGENESIS (MEETING ABS 19/ S (HALOGEHAECD OLEFINS VlHYl AMD ALlYl ANALOGS AND EPOXIDES) (PP 8 21} / STRUCTURAL. PARAMETERS ASSOCIATED WITH C 9 SYHIHESIS ANO REACTIONS OF CHLOROALKENE EPOXIDES 140 lECTROPHlllC REACTIVITY OF THE METABOUCAllY FORMED EPOXIDES / METABOLIC ACItVAIIQN Of CHLORINATED EIHYLENLS DIPiN 8 184 UMINARY REPORT / REACTIONS Of EPOXY l.l 2 TRICmoROETHAKE WITH NUCLEOPHILES EFFECT OF El HANOI ANO VINYL CHLORIDE ON THE INDUCTION Of UVER TUMORS PR[ 3/ OF VINYL CHLORIDE (HEFTING ABSTRACT/ FORMATION OF UHEKO DERIVATIVES OF NUCLEIC ACID BASES IN VIVO BY MI1AB0UIES 175 1C ACUVITY Of D! AND TfllfUNCIlONAL ALPHA CHLQRO ETHERS At OF 1,4 DICMIOROBUUNE 2 IN fCR/HA SWISS MICf r CARCI 113 CHLORUETHANF (ETHYLENE BICHLORIDE) CHlOROETHAHQL t ETHYLENE CHLOSOHYDSlN) VINYL CHLORIDE ANO CYCLOPHOSPHAMIDE (MEf 150317 REVIEW OF SELECTED LITERATURE ON ETHYLENE DlflRQMlDC (EOB) INHALATION DF ETHYLENE DlBROMlDl DURING GESTATION BY RATS AND MICE 7 ETHYLENE 01 BROMIDE IN URBAN AIR 63 0 LIVER (MEETING ABSTft/ BINDING OF THE CARCINOGEN E7HTLENE DlBROMlDE 10 CHROMOSOMAL CONSTITUENTS OF FQRESIOMACH AN 62 POTENTIAL ACTIVATED IN1ERMEDIA1ES OF THE CARCINOGEN ETHYLENE DIBROMIOE WITH PROTEIN AMD ONA IN VITRO (MEETING ABSIRA 299 CARCINOGENIC RISK ASSESSMENT E1HYLENE DlBROMlDE 102 REPAIR OF RAT LIVER DNA IN ViVO DAMAGED BY ETHYLENE OlBflOUIDE 316 ETHYLENE DlBROMlDE (PP 195 209) 113 POSSIBLE MFTABOUC PRODUCT OF 1,2 DICHlOROUHAHt \ ETHYLENE DtCHlORIDEl CHIOROETHANOL (ElHVlENE CHI ORO HYDRIN; VlN 65 FRACTION OF AClIVAFCO CARCINOGENIC INTERMEDIATES OF ETHYLENE OIHAUDES WITH PR01EIN ANO DNA IN MJCE AND FIATS TISSUES 81 R ARQClOR 1254 PRE1KATM/ ACUTE HEPATDlQXICm OT ETHYLtNE VINYL FLUORIDE VINYL CHLORIDE ANO VINYL BROMIDE AFTf 21 PHARMACOKINETICS OF HALOGENATED ETHYLENES (MEETING ABSTRACT] 196 0RMA1I0H OF TRICHLOROETHYLENE AND OTHER CHLORINATED E1HVLENES (MEElING ABSTRACT I / MOLECULAR ASPEClS 10 THE OXIRANE 32 IRREVERSIBLE BINDING OF CHLORINATED ETHYLENES 10 MACROMOllClUES 69 ME1AB0LISM OF HAIOGENAIED ETHYLENES 199 CARCINOGENIC POTENTIAL OF CHLORINATED ETHYLENES TENTATIVE MOLECULAR RULES iPP 171 175) |4G IVITY OF THE. METABOLIC ACTIVATION Of CHLORiMAIED ETHYLENES OEPENOENCE OF MUTAGENIC EffECl ON ELECTROPHILIC RUCI 236 RASI AND ARSENIC COMPARISON WITH CASES OF UNKNOWN ETIOLOGY / DEVELOPMENT OF HEPATIC ANGIOSARCOMA IN MAN INDUCED 8 283 VINYL CHLORIDE A REPORT OF A EUROPEAN ASSESSMENT 57 HLOROETHVIENE TO HEPATIC MICROSOMAL PROTEINS AND 10 EXOGENOUS DNA IN VITRO /' C0VA1EN1 BINDING OF THE CARCINOGEN TRi 241 PRECURSOR LESIONS IN EXPOSED POPULATIONS AS INOLCAIORS OF OCCUPATIONAL CANCER RISK 72 IGLVCOllE ADD IN URINE SPECIMENS OF VINYL CHLORIDE EXPOSED WORKERS OfTERMlNAHON 01 IWIQD IL6 YE CHLORIDE DEPENDENT MUTAGENESIS EFfECIS OF LIVER EXTRACTS AND A FREE RADICAL GENERATING SYSTEM <MEElING ABSIRACI HJ 11/ A HSATOLOGIC EVALUATION OF PLASMA SOLUBLE EXTRACTS OF POLYViNH CHLORIDE PLASTICS JN RAIS (MEElING ABSIRAC 216 MORTALITY AMONG EMPLOYEES OF PVC FABRICATORS 2 Ur HAZARDOUS MATERIALS IN THE A1M05PKERE OF ROBBER FACTORIES MEA5URIMENI 01 SOME POTfRTJA 189 Of I I OICHEOROETHVUNE TOXICITY BY PRCTRWMEHT Of FASTED MALE RATS WITH 2 3 EPOXYPROPAN I 01 ENHANCE MINI 188 CHLOAOETHYLENE IN THE RAT EFFECTS OF SEX AGE ANO FASTING ORAL IQxlCllY Of j I Oi 153 MALIGNANT IRANSTQRMAllOH Of CELLS OF WHOLE EMBRYOS, FETAL BRAIN AND NEWBORN LUNG OF HAMSTER rMEEIJNG ABSIRACFj 148 STEMAIICALLY DURING ALL THIRDS OF PREGNANCY ON THE fflUSES OF CFY RATS EffECIS 01 VINYL CHLORIDE EXPOSURE ALONE 176 OKYUHHY MOUSE SKIN CARCINOGENICITY TESTS OF THE FUME RETARDANTS TRiS(2 3 DIBROMOPRGPU (PHOSPHATE T1 IRAKlSi KYDR 81 Ml HATH' ACUTE HEPATOTOKICITY OF ETHYLENE VINYL FLUORIDE VJNYl CH10R10E ANO VINYL BROMIDE ATI(k AkOCtOR 1/S* P 279 SORPTION OF VINYL CHLORIDE BY SELECTED FOOD CONSTITUENTS 278 vinyl chloride polymers in contact with FOOD 63 N ETHYLENE ClBROW IDE TO CHROMOSOMAL CONST IUIEH1S OF fORESlOMACH ANO LIVER (MEElING ABSIRACI) ' BINDING OF THE CARC! 11 VOLATILE CARCINOGENS OCCURRENCE FORMATION AND ANALYSIS JPP 1943 1949) 60 SPECTRAL EVIDENCE FOR 2.2 3 TROLORO OXIRANE FORMAllON DURING MICROSOMAL lRlCHlQfcOflHVlENE OXILWNDN iMffDNG 4Q BY VINYL CHLORIDE METABOLITES IH VITRO AND 04 VIVO FORMAT ION OF I N**6 EIHENO ADI NO SIN l AlKriAllON OF UNA 38 RIDE ME1ABQUTES IN VITRO AND IN VIVO/ FORMATION OF 3N**4 E1HEN0CYKDLNE MQ1UHS IN SNA BY VIHYL C)UO 37 METABOLITES Of VINYL CHLORIDE (MEETING ABSTRACT; FORMATION OF EIHENO DERIVA1IVES OF NUCLEJC ACID BASES IN VIVO BY 39 RNA| BY METABOLITES OF VINYL CHLORIDE IN VIVO A/ FORMATION OF IMIDAZOl OEAJVATIVES 01 NUCLEIC ACID BASES lUNA AND 125 FOR IN VllftO DETECTION OF MAMMALIAN ENZYME MEDIATED FORMATION OF RUCUVE MElABQUifS MUlAGENlCITY ANU CHRUMUSDUA 116 ENDENT MUTAGENESIS EFFECTS Of UVER EXTRACTS ANO A FREE RADICAL GENERATING SYSTEM IMUHNG ABS1RAC1> i PP 176! 379 CH CANCER IN RATS AND MICE BY HALOGENATED ALIPHATIC FUMIGAWIS INDUCTION OF STOMA 5 CONE HALIDES GAS CHROMATOGRAPHIC ANALYSIS DF HALOGENATED HYDROCARBONS IN SHI 6 TABOUTES IN URINE .1 GAS LIQUID CHROMATOGRAPHIC DEHRMFNAElON OF TRICHlOROlIHUtNl ME 115 INDUCTION OF GENE MUTATIONS ANO GENE CONVERSIONS BY VINYL CHLORIDE MLIABOUlFS IN TIASr 115 S IN YEAS! INDUCTION OF GENE MUTATIONS AND GENE CONVERSIONS 6Y VINYL CHLORiOt MLUBOLIIl 126 GENETIC AClIVIlY Of AUYL CHlORiDf 101 (MEETING AN EXPERIMENTAL APPROACH FOR EVALUATING GFNET1C ANO EPIGENETIC CONIRlBUllGHS 10 CHEMICAL CARCINOGEmSIS 14? OiBUTH PHTHALATE AND TRICHLOROETHYLENE ON SACC' GENETIC AND TOXICOLOGICAL EffECIS Of ALPHA BENZENE HUACHlORtDL 320 1131' GENETIC EFFECTS ASSOCIATED WITH INDUSTRIAL CHEMICALS jPP JC' 111 MMALIAH METABOLIC AC1IVAT10N / EVALUATION Of THE GENETIC EFFECTS INOUCEO BY VJNYL CHLORIDE MONOMER jvCMi UNDER MX 321 EFFECTS Of ENVIRONMENTAL CHEMICALS ON THE GENETIC REGULATION OF MICROSOMAL ENZYME SYSTEMS 134 Of INDUSTRIAL COMPOUNDS EVALUATED BY MEANS OF YEAST GENETIC SYSTEMS IMEETJNG ABSTRACTj MLffAGENlCllt 150 INHALATION OF ETHYLENE DtBROMIDE DURING GESTATION BY RATS AND MICE 127 TVPHLMOfmw I ACTIVATION THROUGH CONJUGATION WITH GlUlAIHION IN VI1RQ 1HE MUTAGENIC EFFECT Of I 2 OJOUOSOETHAh 89 EDOCTASE IN UVER Of RATS EXPOSED 10 VI ELEVATED GLUTATHIONE CONTENT GlUIAIHlGNE S TRANSFERASE AND GlUTAlHIOhf R 97 CEO DEACTIVATION OF UVER ENDOPLASMIC RETICULUM AND GLUIATHJONE DtPLEIlON (MEETING ABSTRACT] tfllCHlOBOllHYlfftf >N 95 DIAC1 tVAT ION OF CY10CHFI0ME P 450 AND LOSS OF UVER CLUTAIHJONE IN VIVO IRlCHIOROE iHYlfNE JNDlfClO 89 glutathione content giutaihlone s transferase anq GLUTAIHIONE REOUCTASE IN UVER Of RATS EXPOS!0 10 vINU CH(CiRIDl 89 RATS EXPOSED TO VI ELEVATED GLUTATHIONE CONTENT GLUTATHIONE S TRANSFERASE ANO GLUTAIHIONE REDUCTASE IN tIVtFr Of 71 JOE EXPOSURE ASSQCIAIEO LIVER URINARY AND TISSUE GLYCOSAMINOGLYCAN PATTERNS IN ANGIOSARCOMA AND OTHER VINU CHI OR 214 MONOMER IN THE MANUFACTURE OF POlVVJNYl CHLORIDE JN GREAT BRITAIN MQRIALI1Y EXPERlENCl OF WORKERS EXPOSED 10 vINt 217 UORTAltlY AND CANCER MORBIDITY IN A GROUP Of SWEDISH VCM AND P)C PRODUCTION WONHfRS 177 i WEE I CARCINOGENICITY ANO CHEMICAL REACTION WUH GUANINE OF I ChlOROPROPENE AND TWO Of IIS POTENllAw MUABOLlUS 5 1C ANALYSIS Of HALOGENATED HYDROCARBONS IN SILICONE HAUDES GAS CHROMA 10GRAPH L99 AL CORRELATIONS OF CARCINOGENIC ANO MUTAGENIC ALKYL HALIDES (PP 163 17b $IRUCTUR UCC 106398 31? :3? n m 179 141 136 69 n 162 5 343 302 69 124 3 9/ 345 325 103 302 194 231 8) 4 353 356 ISS IS? m 253 ?73 201 23Q 290 166 266 296 226 294 162 44 262 235 232 236 IOG 212 58 98 95 12 45 57 54 91 16? 61 96 61 197 66 14? 74 161 79 239 239 93 233 ?S6 124 73 76 107 112 5 162 *UDE4U CHLORIDE vtNYt THE MUTAGENICITY ANO ONA MODIFYING EFFECT Of LIVEN ENDOPLASMIC RETICULUM lAfiGET SITE OF INDUSTRIAL MUTAGENS ANO POTENTIAL MUTAGENS 1 INQUCllQH Of STOMACH CANCER IN RATS AND MICE BY MUTAGENICITY Of THE MUTAGENIC ACTIVITY Of METABOLISM Of PHARMACOKINETICS Of CARCINOGENICITY STUDIES ON gas chromatographic analysis op HAIOGENATED SATURATED HYDROCARBONS / potential HALOGENATED UNSATORATEO HYDROCARBONS POTENTIAL SAL MO DETECTION OF ELECTROPHILIC METABOLITES Of ALKYLATING ANO MUTAGENIC METABOLITES Of OCTORAl PARAMETERS ASSOCIATED WITH CARCINOGENESIS C MEIABQUSM ANO MUTAGEKLCtlV Of MUIAGINJC ANO CARCINOGENIC RISKS ASSOCIATED WITH INDUSTRIAL CARCINOGENIC AND MUTAGENIC CHEMICALS II INDUSTRIAL CARCINOGENIC AND MUTAGENIC CHEMICALS I MLCAL STRUCTURE REACTIVITY AND CARCINOGENICITY Of ASSOCIATIONS Of CANCER MORTALITY WITH THE TOXICITY Of SOME ED SPECIROPHOIOMETRIC IDENTIFICATION OF CHLOROFORM OF WHOLE EMBRYOS FETAL BRAIN ANO NEWBORN LONG OF H1CROSOME MfOtATED MUTAGENESIS Of A CHINESE MAINTAINED IN VITRO FOR 2 I '2 YEARS / tPP 79i/ MALIGNANT TRANSFORMATION OF A BABY BE PROTECTED1' PUBLIC HEALTH ROUNDS AT THE CAL EXPERIMENTAL STUDY i S THE REGULATION OF VINYL CHLORIDE {MEET/ PUBLIC EPIDEMIOLOGICAL STUDIES OF VINYL CHLORIDE lHf POTENTIAL GENS AND ANllCARCINOGENS IN REDUCING ENVIRONMENTAL THE VINYL CHIQRIOE MONOMER IBlE USE OF A BIOLOGICAL EAPOSUfiE TEST TO OHERMINE OCCUPATIONAL SAFETY ANO UBHC HEALTH ROUNDS AT THE HARVARD SCHOOL Of PUBLIC DRINKING WATER ANO HENCE Of l OCCURRENCE Of HEPATOCELLULAR TUMORS AND METABOLISM OF VINYL CHLORIDE DESTRUCTION OF THE L HYPERTENSION IN VINYL CHLORIDE MONOMER WORKERS A CLINICAL ANO MORPHOLOGIC FEATURES Of NTl CHLORIDE MORPHOLOGICAL DESCRIPTION OF THE L/ T AND ARSENIC COMPARISON WITH D DEVELOPMENT Of OK SYNERGISM AND ANTAGONISM / DAMAGE TO PLANT/ INTERACTION BETWEEN TRICHLOROETHYLENE AND INI 1IAL FEATURES OF VINYL CHLORIDE (VC) RBITAl / ACUTE IDE THE INTERACTION OF VINYL CHLORIDE WITH RAI LENT BINDING OF THE CARCINOGEN TRICHLOROETHYLENE TO ABOLITES Of THE CARCINOGEN TRICHLOROETHYLENE IN RAT ALEIN (BSP)/ VINYL CHLORIDE INDUCED DEPRESSION OF BKOHOCmm UNDER THE INFLUENCE OF? OCCURRENCE OF MECHANISM FOR TRICHLOROETHYLENE WCT|/ ACUTE TRICHLOROETHYLENE VINYL BROMIDE AFTER AROCLOR 1254 PRETREATU? ACUTE FOR THE SUBCELtULAR SITE OF/ 1,1 OlCHLOROETHYLENE D BINDING OF "HC UOlOACT/ 1L OlCHLOROETHYLENE GENETIC ANO TOXICOLOGICAL EFFECTS OF ALPHA BEN2ENE TERM TOXICITY AND REPRODUCTION STUDIES IN RATS WITH RESULTS OF A TWO TEAR CHRONIC TOXICITY STUDY WITH CHRONIC TOXICITY AHO REPRODUCTION STUDIES 01 ENS IN VINYLCHLOfllOE DISEASE / EN2YME ORIDE DISEASE S ENZYME HISTOCHEMICAL RAT LUNG/ SOME BIOCHEMICAL AND L CHLORIDE MONOMER I MEETING A6S1RAC1)/ 1IIOLOG1C STUDY DT BLOOD SCREENING TESTS AND ILLNESS ENIC METABOLI1ES OF HALDGENATED OLEFINS PRODUCED BY ISM OT TRICHLOROETHYLENE ANO TETAACHLOROLTHYLENE IN EVIDENCE FOR EXISTENCE IN DE IH S TYPHIHURIUM STRAINS/ OUIES CHLOIGOXtRANE ANO CHLOBOACETAtOEHtOE MONOMER GAS CHROMATOGRAPHIC ANALYSIS OF HAIOGENATED CARCINOGENIC!IY STUDIES ON HAIOGENATED HALIDES CARCTNOGLNlCirY VINYL BROMIDE VINYL CHLORIDE AHO VJN HALOALKANES HALOCARBON METABOLITES HAIOCENAHD ALIPHATIC DERIVATIVES HAIOCLNATEO ALIPHATIC FUMIGANTS HAIOGENAUO ALKANES ANO THEIR DERIVATIVES HALOCENATEO COMPOUNDS FOUND IN CHLORINATED DRINKING WATER HAIOGENATED ETHYLENES HALOGENATED ETHYLENES (MEETING ABSTRACT) HAIOGENATED HYDROCARBONS HALOGENATED HYDROCARBONS IN SILICONE HALIDES HAIOGENATED INDUSTRIAL CARCINOGENIC AND MUTAGENIC CHEMICALS II KALOGENAU1F INDUSTRIAL CARCINOGENIC AND MUTAGENIC CHEMICALS I HALOGENAUO GUI IKS WITH 4|4 N!TRQBtH2YUPYRtDIHl (NBP) OR WITH HALOGENATED OLEFINS PRODUCED BV HUMAN AND ANIMAL IISSUES HALOGENATED OLEFINS VINYL ANO ALLYL ANALOGS ANO EPOXIDES) (PP HALOGENATED OLEFINS A COMPARISON OF STRUCTURE AND ACTIVITY HAIOGENATED OLEFINS HALOGENATED SATURATED HYDROCARBONS / POTENTIAL HAIQGENATEO HALOGENATED UHSATURATED HYDROCARBONS / POTENTIAL HALOGENATED HAlOHVDROCAIBONS CHE HALOMETHANCS M DRINKING WATER HAlOMETHANES Ul MICE HALQTHANL METHOXYFLURANE. TETRACHLOROETHYlENE ANO TRICHLOROETH HAMSTER LMEETIHG ABSTRACT) / IN VITRO MALIGNANT TRANSFORMATION HAMSTER CELL LINE BY VARIOUS CHEMICALS (MEETING ABSTRACT) KAMS1ER CELLS UN1REA1ED ANO TREATED WITH CHEMICAL CARCINOGENS HAMSTER LUNG CELL LINE BY 2 CHlOROBUlADlENE (MEETING ABSTRACT) HARVARD SCHOOL OF PUBLIC HEALTH VINYL CHLORIOE CAN THE WORKER HEALTH DAMAGE BY TRICHLOROETHYLENE AN EPIDEMIOLOGICAL AND ClINI HCAL1H DECISION MAKING AND THE LEGAL PROCESS IN THE UNITED STATE HEALTH EFFECTS III THE UNITED STATES (MEE1IKG ABSTRACT) HEALTH EFFECTS 06 VINYL CHLORIDE HEALTH HAZARD OF SUBSTANCES LEACHED FROM PLASTIC PACKAGING HEALTH HAZARDS / EVALUATION OF A POSSIBLE ROLE FOR ANTIMUTAGENS HEALTH PROBLEM HEALTH S1AN0AR0S FOR WORKERS ' THE POSS HEALTH STANDARDS TOXIC AHO HAZARDOUS SUBSTANCES HEALTH VINYL CHLORIDE CAN THE WORKER BE PROTECTED1 !P HEALTH RECOMMENDATIONS OF THE NATIONAL ACAOEMY OF SCIENCES HEMANGIDSARCOMA IN RATS INHALED U DlBROMOETHANE UNDER THE INFL HEME OF HIGHLY PURIFIED LIVER MICROSOMAL CYTOCHROME P 459 BY A M HEMODYNAMIC STUDY ' PORTA HEPATIC ANGIOSARCOMA IN VINYL CHLORIDE WORKERS HEPATIC ANGIOSARCOMA IN WORKERS FOLLOWING CHRONIC EXPOSURE 10 VI HEPATIC ANGIOSARCOMA IN MAN INDUCEO 8V VINYL CHLORIDE THQRQIftAS HEPA1IC CELLULAR MEMBRANES BY CHLORINATED OlfflHS WITH EMPHASIS HEPATIC DISEASE AMONG WORKERS AT A VINYL CHLORIDE POLYMERIZATION HEPATIC ENDOPLASMIC RETICULUM (MEETING ABSTRACT) (PP 69 70) HEPA1IC INJURY (MEETING ABSTRACT) HEPATIC INJURY BV VINYL CHLORIDE IN RAIS PRETREATED WITH PHENOBA HEPATIC MACRDMOtECULAR BINDING FOLLOWING EXPOSURE TO VINYL CHLOR HEPATIC MICROSOMAL CV10CHR0ME MW IN VITRO HEPATIC MICROSOMAL PROTEINS AND TO EXOGENOUS ONA IN VITRO / COY HEPATIC MlCRQSOMES / COVALENT INTERACTION OF MET HEPATIC NON-PROTEIN SUlFHYDRYl CONTENT ANO EFFECTS ON BROMOSULPH HEPATOCELLULAR TUMORS ANO HEMANGIDSARCOMA IN RATS INHALED 12 Dl HEPATOTOXICITY (MEETING ABSTRACT) HEPATOTOXICITY AND INDUCTION OF MICROSOMAL ENZYMES (MEETING AB5T HEFAJOTOXlCm OF ETHYLENE. VINYL FLUORIDE VINYL CHLORIDE. AND HEPAT010XICITY EFFECT OF ALTERED lHYROID FUNCTION AND EVIDENCE HEPATOTOXICITY PROPOSED MECHANISM OF ACTION ANO DISTRIBUTION AN HEAACHLORIOE. OlBUTYL PHIHALATE ANO TRICHLOROETHYLENE ON SACCHAR HEXACHLORO (1.3) BUTADIENE. / SHORT HEXACHLOROBUTAOtENE RATS HEXACHLQROBUTAOfEHE IN RATS HlSTOCHEMICAl, H1ST0METRICAL AND ULTRASTRUClUflAl STUDIES OF SPtE HlSTOMETIICAL AND ULTRASTRUCTURAL STUDIES OF SPLEENS IN V1NYLCHL HISTOPATHOLOGICAL CHANGES INDUCED BY POLYVINYL CHLORIDE DUST IN HISTOPATBQLOGV DF LIVER LESIONS ASSOCIATED WITH EXPOSURE TO VINY HISTORIES AMONG CHEMICAL WORKERS INVOLVED IN 1HE MANUFACTURE OF HUMAN AIO ANIMAL TISSUES / ALKYLATING AND MUTAG HUMAN SUBJECTS / METABOL HUMAN TISSUES OF MONOMERS FOR PLASTICS AND RUBBER MANUFACTURE HUMAN RAT AW) MOUSE LIVER MEDIATED MUTAGENICITY OF VINYL CHLOR) HYDRATE l VINYL CHLORIDE MUTAGENICITY VIA THE METAB HYDROCARBONS IN SILICONE HALIDES HYOROCARBQNS 303 302 363 26? 175 4 ?43 256 HA 39 262 224 241 93 331 111 236 159 151 115 184 96 163 174 154 3D3 M2 203 320 103 134 104 208 292 307 295 242 322 24 119 4 77 13 66 150 149 29 160 171 167 14 195 203 71 99 8? 85 18? 59 190 65 54 62 45 36 65 62 232 234 269 32 33 65 130 16 31 29 24 C ANO MUTAGENIC CHEMICALS II HALOGENATEO SATURATED ANO MUTAGENIC CHEMICALS I HALOGENATED UNSATURATED POSTAL UDY PQR1AL ALPHA CHLQRO ETHERS MO Of 1 4 DICHLOROBUTENE 2 IN LOROElHYlENE VAPOR INFRARED SPECIROPHOIOMETRIC E STUDIES UNDERTAKEN ON PERSONS AND ANIMALS WITH VC AN EPIDEMIOLOGIC STUDY Of BLQQO SCREENING TESTS AND L CHROMOSOME STUDIES OK PERSONS WITH AND WITHOUT VC BODIES OF VINYL CHLORIDE IN VIVO A/ fOAMAMON OF 1SEASE f LUNG CANCER PRECURSOR LESIONS IN EXPOSED POPULATIONS AS SOME BIOCHEMICAL ANO HISTOPATHOLOGICAL CHANGES CARCINOGENESIS C AClIVATlON EVALUATION OF THE GENETIC EFFECTS ifH C DEVELOPMENT OF HEPATIC ANGIOSARCOMA IN MAN PULMONARY TUMORS BSTRACTl, VINYL CHLORIOE EXPOSURE OE METABOLITES IN YEAST; EFFECT OF ETHANOL AND VINYL CHLORIDE OH THE ACUTE TRICHLOROETHYLENE HEPATOTOXICITY AND ORCANOHAIIDES, HA1IC fUMIGANTS; ORMING ACTIVITIES OF TRICHLOROETHYLENE AND PROPOSED SATURATED HYOROCARBONS / POTENTIAL HALOGENATED UNSA1URAKD HYDROCARBONS f POTENTIAL HALOGENAUO TIC ANO NON NEOPLASTIC OCCUPATIONAL INJURIES DUE 10 GENETIC EFFECTS ASSOCIATED WITH METABOLITES {MEE11NG ABSTRACT) / MUTAGENICITY OF (MEETING ABSTRACT); MUlAGENICITY OF METABOLITES (MEETING ABSTRACT) 7 MUTAGENICITY OF EMPLOYEE/ VINYL CHLORIDE EXPOSURE IN A CONTROLLED EXPERIENCE IN AIIC DERIVATIVES; INYICHIORIDE MONOMER (VC/ METABOLIC APPROACH TO AGENICHY OF WASTE PRODUCTS FROM THE VINYL CHLORIDE ANE METHQXYFLURANE 1ETRACHLOROE1HYLENE ( VAPOR EFFECTS OF POLYVINYL CHLORIOE FATE OE **14 C VINYL CHLORIDE FOLLOWING BUTION ANO BINDING OF **14C RADJOAClIVllV FOLLOWING CE / OTEHTIAl OF BETA CHlORQPREHE (2 CHLQROBUTADIENE / HLOROETHVLENE METABOLISM / THE USE OF D IN THE DRINKING WATER OR ADMINISTEREO BY REPEATED F HEPAIOCELiULAB TUMORS AND HEMANGIOSARCOMA IN RATS PRELIMINARY STUDIES QF THE FATE OF tFFECl OF VARIOUS TREATMENTS ON TOXICITY OF STEM FOR NEOPLASTIC AND NON NEOPLASTIC OCCUPATIONAL ANO OTHER VINYL CHLORIDE EXPOSURE-ASSOCIATED LIVER INITIAL FEATURES OF VINYL CHLORIDE (VC) HEPATIC IS OH DOSE RESPONSE IN VINYL CHLORIDE INDUCEO LIVER ACUTE HEPATIC 0 F0HC1L0H AND EVIDENCE FOR THE SOBCEUULAR SITE OF TICULUM (MEETING ABSTRACT) (PP 69 70)/ CD (PP 10)/ POSSIBLE METABOLIC DIHALIDES WITH PROTEIN AND DNA IN MICE AND RATS / N RAT HEPATIC MICROSOMES / COVALENT EN ETHUENE OIBROMlDE WITH PROTEIN AND ONA IN VI/ ROME P450 IN VI1RO/ THE ANO RAIS / INTERACTION OF ACTIVATED CARCINOGENIC AND DNA IN VI/ INTERACTION OF POTENTIAL ACTIVATED L CHANGES OF THE LIVER AFTER CHRONIC VINYL CHLORIDE STRUCTURE OF LIVER DAMAGE IN CHRONIC VINYL CHLORIDE DE ITS IMPACT ON OCCUPATIONAL MEDICINE PRACTICE IK LIVER CONSTITUENTS IN VIVO ANO IN VITRO/ METABOLISM Of TRICHLOROETHYLENE BY THE NE ON SALMONELLA TVPHlMURFUM (I ACTIVATION BV THE AXE ANO RATE OF METABOLISM OF VINYL CHLORIDE BY THE ISTRIBUTlON OF METABOLITES OF BENZOPYRENE IN THE THE USE OF INHALATION TECHNIQUES TO ASSESS THE METABOLIC APPROACH TO INDUSTRIAL POISONING BLOOD HYDROCARBONS POTENTIAL HALOGENATED INDUSTRIAL CARClNOGENl HYDROCARBONS POTENTIAL HA10CCNAIEP INDUSTRIAL CARCINOGENIC HYPERTENSION IN VINYL CHLORIOE PRODUCTION WORKERS HYPERTENSION IN VJNVL CHLORIDE MONOMER WORKERS A HEMODYNAMIC SE ICR HA SWISS MICE CARCINOGENIC ACTIVITY Of 01 ANO IRIFUNCTIU IDENTIFICATION Of CHLOROFORM HAlGlHANE METHOAYFLURANE TETRACH ILLNESS LMEETING ABSIRACI) EXTERNAL CHROUOSOM ILLNESS HISTORIES AMONG CHEMICAL WORKERS INVOLVED IN IHE MANUTAC ILLNESS AND ON LC EXPOSED ANIMALS MUTAGENICITY Of VINYL CHID IMIDAZOL DERIVATIVES Of NUCLEIC ACID BASES I DNA AND RNAf BY MEIA IMMUNOLOGICAL MECHANISMS IN THE PATHOGENESIS Of VINYL CHLORIDE D INCIDENCE AMONG CHiCRQPRENE HANDLING WORKERS INDICATORS Of OCCUPATIONAL CANCER RISK INDUCED BY POLYVINYL CHLORIDE DUST IN RAI LUNG INDUCED BY TRACE CONTAMINANTS IN P01ABU WATER INDUCED BY VINYL CHLORIDE MONOMER tYCM) UNDER MAMMALIAN MEIABOll INDUCED BY VINYL CHLORIDE THOROTRASI AND ARSENIC COMPARISON W INDUCED IN MICE BY VINYL CHLORIDE MONOMER INDUCED LYMPHOCYTE TRANSFORMATION IN SPLENIC CULTURES (MEETING A INOUCIIQH OF GENE MUTATIONS AND GENE CONVERSIONS BY VINYL CHiORI INDUCTION Of LIVER TUMORS PRELIMINARY REPORT INDUCTION Of MICROSOMAL ENZYMES (MEETING A6SIRACT) INDUCT ION QF PULMONARY ADENOMAS IN STRAIN A MICE BY SUBSIlTUlEO INDUCTION QF STOMACH CANCER tft RATS AHO MICE BY HAIOGENAUO ALIP INDUSTRIAL ALTERNATIVES TRANSf INDUSTRIAL CARCINOGENIC ANO MUTAGENIC CHEMICALS II HALOGENATED INDUSTRIAL CARCINOGENIC AND MUTAGENIC CHEMICALS I HAIOGENATED INDUSTRIAL CHEMICALS (MEETING ABSTRACT) , MEDICAL SURVEILLANCE INDUSTRIAL CHEMICALS {PP 100 113) INDUSTRIAL COMPOUND VINYL CHLORIDE. S1YRENE AND THEIR POSSIBLE INDUSTRIAL COMPOUNDS EVALUATED BY MEANS OF YEAST GENE1IC SYSTEMS INDUSTRIAL COMPOUNDS VINYL CHLORIDE STYRENE ANO THEIR POSSIBLE INDUSTRIAL ENVIRONMENT A LONG IERU MORTALITY EXPERIENCE IN 594 industrial exposure CONTROL INDUSTRIAL HAZARDS DUE 10 YlNYl CHLORIDE INDUSTRIAL MEDICINE AND ViNYL CHLORIDE INDUSTRIAL MONITORING A CYTOGENETIC APPROACH INDUSTRIAL MUTAGENS AND POTENHAL MUTAGENS I HALOGENATED ALIPH INDUSTRIAL POISONING BLOOD KINETICS AND DISTRIBUTION Of *I4CV INDUSTRIES (MEETING ABSTRACT) ; THE MUl INFRARED SPECTROPHOTOUEfRIC IDENTIFICATION OF CHLOROFORM HAL01H INGESTION BY DOGS INHALATION EXPOSURE IN RAIS INHALATION EXPOSURE IN RATS / I I OlCHLOROETHYLENE HEPATOTOXlCI INHALATION OF ETHYLENE DI6R0MIDE DURING GESTATION BY RAIS AND Ml INHALATION STUDIES TO EVALUATE THE TERATOGENIC AND EMBRYOTOXIC P INHALATION TECHNIQUES TO ASSESS THE KINElIC CONSTANTS OF 1,1 OlC INHALATION TOXICITY OF VINYL CHLORIDE ANO VINYLIQINE CHLORIDE INHALATION / IN1ERIM RESULTS Of TWO YEAR TOXICOLOGICAL STUDIES INHALED I 2 DlBROMOETHANE UNDER THE INFLUENCE OF OlSULflRAM IRTA INHALED VINYL CHLORIDE MONOMER (VCM> IN RAIS INHALED VINVLIDENE CHLORIDE INJURIES OUE TO INDUSTRIAL CHEMICALS (MEETING ABSTRACT) ; MEDIC INJURY (MEETING ABSTRACT) {PP 170) / URINARY ANO TISSUE GLYC INJURY (MEETING ABSTRACT) INJURY AND TUMORS IN MICE (MEETING ABSTRACT) / SOME ASPEC INJURY BY VINYL CHLORIDE IN RATS PREHEATED tfllH PHENOBARBlTAL INJURY / 1.1 DICHLOROL1HYLENE HEPATOTOXICITY EFFECT DF ALILRED INTERACTION BETWEEN TRICHLOROETHYLENE ANO HEPATIC ENDOPLASMIC RE INTERACTION BETWEEN STYRENE AND ORGANIC SOLVENTS {MEETING ABS1NA INTERACTION OF ACTIVATED CARCINOGENIC INTERMEDIATES OF EIHYLENf INTERACTION OF METABOLITES OF THE CARCINOGEN TRICHLOROETHYLENE I INTERACTION Of POTENHAL ACTIVATED INTERMEDIATES OF TH CARCJNGG INTERACTION OF VINYL CHLORIDE WITH RAT HEPATIC MICROSOMAL CVTOCH INTERACTIONS OF VINYL CHLORIDE W)1H RAT LIVER DNA IN VIVO INTERMEDIATES OF ETHYlENE Dl HAL IDES WITH PROTEIN ANO DNA IN MICE INTERMEDIATES OF THE CARCINOGEN 11HYLENE 01BRQM10E WITH PROTEIN INTOXICATION / MORPHOLOGlCA INTOXICATION / ULIRA IRAN / VINYL CHIOS) IRREVERSIBLE BINDING Of CHLORINATED ETHYLENES TO MACROMOlECULES IRREVERSIBLE BlNOING OF **I4C LABELLED TRICHLOROETHYLENE TO MICE ISOLATED PERFUSED LUNG ISOLATED PERFUSED RAT LIVER / IHE MUTAGENIC EFFECT OF IZ0IOU ISOLATED PERFUSED RAT LIVER PREPARATION / UPT ISOLATED PERFUSED RABBIT LUNG PREPARATION (MEEltNG ABSTRACT) KINETIC CONSTANTS OF 1 1 OtCHlOROETHYLENf METABOLISM KINETICS AND DISTRIBUTION Of *M4C VINYLCHLOfilUE MONOMER {VCM UCC 106399 260 m 28D 298 2/3 231 241 23/ l 106 11/ 289 315 94 63 23? 265 $9 259 204 33 234 260 104 102 9/ 96 116 96 213 m 206 201 /I 8/ 233 44 19 i 9 16 184 90 130 215 261 208 2?4 234 15? 153 31 93 56 157 266 151 246 246 74 17 64 56 32 106 105 189 35? 153 125 111 50 236 250 284 2J4 25b 210 7b POLYVINYL CHLORIDE PRODUCTION WORKERS CLINICAL AND LAPAROSCOPIC ASPECTS f LIVER DISEASE IN MORTAL IIV AMONG LAUNDRY AND DRY CLEANING WORKERS (MEETING ABSTRACT) 1HE POrCNIIAL HEALTH HAZARD Of SUBSTANCES LEACHED FROM PLASTIC PACKAGING LEGAL DECISIONS AND OPINIONS IN POLLUTION CASES RlOE (MEET/ PUBLIC HEALTH DECISION MAKING AND THE LEGAL PROCESS IN THE UNITED STATES THE REGULATION OF VINYL CHLQ TING ABSTRACT / HISTOPATHOLOCV OF LIVER LESIONS ASSOCIATED WITH EXPOSURE TO VINYL CHLORIDE MONOMER (HEE CER RISK / PRECURSOR LESIONS IN EXPOSED POPULATIONS AS INDICATORS OF OCCUPATIONAL CAN 10 VtNH CHLORIDE MORPHOLOGICAL DESCRIPTION OF THE LESIONS t HEPA1IC ANGIOSARCOMA IN WORKERS FOLLOWING CHRONIC EXP VINYL CHLORIDE DOMINANT LETHAL STUDIES IN MALE CD 1 MICE RlOE OR VINYUDENE CHLORIDE/ A DOMINANT LETHAL STUDY IN MALE RATS AFTER REPEATED EXPOSURES TO VINYL CHlO REVIEW OF SELEC1E0 LITERATURE ON ETHYLENE OIBROMIOE (EDO) VINYL CHLORIDE POISONING IN THE USSR LITERATURE SURVEY VINYL IDENE CHLORIDE A REVIEW OF THE LITERATURE ENE CHLORIDE INDUCED ULTRASTRUClURAL CHANGES IN RAT LIVER (MEETING AB51RACT) / VINYliO MIDI TO CHROMOSOMAL CONS1MUENIS OF FORESIOMACH AND LIVER (MEETING ABSTRACT! / BINDING OF THE CARCINOGEN ETHYLENE D MORPHOLOGICAL CHANGES OF THE LIVER AFTER CHRONIC VINYL CHLORIDE INTOXICATION PRENEOPLASTIC AND COLLAGENI2ING CHANGES IN 1HC LIVER AND BLOOD CLOTTING IN PVC WORKERS l.l MftICHLOROUHANE AND IRtCHlGROElHYlENl ON RAT LIVER AND BRAIN / BIOCHEMICAL AND TOXICOLOGICAL EFFECTS OF COMB SCINTIGRAPHY Of LIVER AND SPLEEN IN VINYL CMQBIDE WORKERS ACT) (PP 425 426)/ PRIMARY LIVER CANCER DETECTION IN VINYL CHlORlOE WORKERS (MEETING ABSTfl BINDING Of **14C LABELLED lRlCHLOROETHYlENE 10 MIC! LIVER CONSTITUENTS IN VIVO AND IN VITRO , IRREVERSIBLE UL'U&TRUCILWE Of LIVER DAMAGE IN CHRONIC VINYL CHLORIDE INTOXICATION AND LAPAROSCOPIC ASPECTS/ LIVER DISEASE IN POLYVINYL CHLORIDE PRODUCTION WORKERS CLINICAL VINYL CHLORIDE ASSOCIATED LIVER DISEASE REPAIR OF RAT LIVER ONA (N VIVO DAMAGED BY ETHYLENE DIBROMIDE BSTRAC/ t$y 1RICHLOROETHVLENE INDUCEO DEACTIVAIION Of LIVER ENDOPLASMIC REUCULUM AND GLUTATHIONE DEPLETION (MEETING A LIVER ENDOPLASMIC REUCULUM TARGET SITE OF HAIOCAABON METABOUT VINYL CHLORIDE DEPENDENT MUTAGENESIS EFFECTS Of LIVER EXTRACTS MID A FREE RADICAL GENERATING SYSTEM (MEETING AB NOUCEO DEACTIVAIION Of CYTOCHROME PASO AND LOSS Of LIVER GLUTATHIONE IN VIVO / TRICHLOROETHYLENE I UN CASES OF ANGIOSARCOMA 01 SHE LIVER IN SHAWINIGAH QUEBEC TEN CASES OF ANGIOSARCOMA OF SHE LIVER IN VINYL CHLORIDE WORKERS IN CANADA ANGIOSARCOMA Of THE LIVER IN VINYL CHLGflfDE/PGLYYINYL CHLORIDE WORKERS OT THE N1QSH REGISTER / ANGIOSARCOMA Of THE LIVES IK VINYL CHUMM/E/POttYUm CHLORIDE WORKERS 197/ UPDATE ARCQMA AND OTHER VINYL CHLORIDE EXPOSURE ASSOCIATED LIVER INJURY (MEETING ABSTRACT) (PP I/O) URINARY AND TlSSU ASPECTS OH DOSE RESPONSE IN VINYL CHLORIDE INDUCED LIVER INJURY ANO TUMORS IN MICE (MEETING ABSTRACTj / SOME (MEETING ABSTRACT) t HLSTOPATHOLOGV Of LIVES LESIONS ASSOCIATED WITH EXPOSURE TO VINYL CHLORIDE MONOMER HLORIDE DESTRUCTION OF THE HEME OF HIGHLY PURIFIED LIVER MICROSOMAL CYTOCHROME P 450 BY A METABOLITE / METABOLISM TION TO PROTEIN ALKYLATING METABOLITES IN VITRO / LIVES MICROSOMAL UP1AKE OF **I4C VINYL CHLORLOE AND TRAHSFORMA 1A1HIONE S TRANSFERASE AMO GLUTATHIONE REOUCTASE IN LIVER OF RATS EXPOSED TO VINYL CHLORIDE (MEETING ABSTRACT) LISM OF VINYL CHLORIDE BY THE ISOU1ED PERFUSED RAT LIVES PREPARATION 1 OF ETHANOL AND VINYL CHLORIDE ON THE INDUCTION OF LIVES TUMORS PRELIMINARY REPORT / UPTAKE ANO RATE Of METABO EFFEC ORACE1ALDEHYDE ON ONA SYNTHESIS IN REGENERATING RAT LIVES ( THE EFFECT OF VINYL CHLORIDE MONOMER CHLQROETHYlCNE OX MURIUM II ACTIVATION BY lHl ISOLATED PERFUSED RAT LIVES / THE MUTAGENIC EFFECT OF L2 DICHlOROCTHANE ON SAlMONELL ANGIOSARCOMA OF THE LIVES AN EPIDEMIOLOGIC SURVEY RESULTS OF A STUDY Of 17 CASES Of LONG TERM EXPOSURE TO VINYL CHlORlOE EXPOSURE IN A CONTROLLED INDUSTRIAL ENVIRONMENT A LONGTERM MORTALITY EXPERIENCE IN 594 EMPLOYEES / VINYL CHLORIDE LUNG CANCER INCIDENCE AMONG CHLOROFRENE HANDLING WORKERS OCCUPATIONAL LUNG CANCER (PP 25 61) MALIGNANT TRANSFORMATION OF A BABY HAMSTER LUNG CELL LINE BY 2 CHLOROBUTAOIENE (MEETING ABSTRACll (PP 78) Of CELLS Of WHOLE EMBRYOS FETAL BRAIN AND NEWBORN LUNG OF HAMSTER lUEETlNG ABSTRACT) / IN VITRO MALIGNANT TRANSFO S OF BEN20(AlPYRENE IN THE ISOLAHD PERFUSED RABBIT LUNG PREPARATION {MEEIkNC ABSTRACT) ' DISTRIBUTION Of MEEABOUT L CHANGES INDUCED BY POLYVINYL CHLORIDE OUST IN RAT LUNG ; SOME BIOCHEMICAL AND HISTQPATHOLOGICA OUStt Of TRICHLOROETHYLENE BY THE ISOLATED PERFUSED LUNG / METAB ONCOGENIC RESPONSE OF RAE SKIN LUNGS AND BONES TO VINYL CHLORIDE MEETING ABSTAAClk SACT) VINYL CHLORIDE EXPOSURE INDUCED CYTOGENETIC INVESTIGATIONS ON LYMPHOCYTE TRANSFORMATION 1ESTS IN VINYL CHLORIDE (VCl WORKERS ( LYMPHOCYTE TRANSFORMATION IN SPLENIC CULTURES (MEETING ABSTRACT) LYMPHOCYTES FROM WORKERS UPOSED TO VINYL CHLOSlOE (MEETING ABST CYTOGENETIC INVESTIGATIONS ON LYMPHOCYTES FROM WORKERS UPOSED TO VINYL CHLORIDE LENE DURING THE DEGREASING OF SMALL COMPONENTS IN A MACHINERY PLANT EVALUATION OF EXPOSURE TO TRICHLOROETHY HEPATIC MACROMOLECULAR BINDING FOLLOWING EXPOSURE TO VINYL CHLORIDE DMOETHANE MACROMOLECULAR BINDING ANO METABOLISM OF THE CARCINOGEN I 2 D1BR C OF 1HE CARCINOGEN TRICHLOROETHYLENE TO CELLULAR MACftOMOlECULiS I MEETING ABSTRACT) / MICROSOME DEPENDENT COVALE IRREVERSIBLE BINDING OF CHLORINATED ETHVLENES TO MACROMOLECULES VINYL CHLORIDE DOMINANT LETHAL STUDIES IN MALE CO I MICE NE CHLORIDE r A DOMINANT LETHAL STUDY IN MALE RAIS AFTER REPEATED EXPOSURES TO VINYL CHLORIDE OR VINYL IDE OICHLOROL1HYLENE TOXICITY BY PAETREATMENT OF fasieo MALE RATS WITH 21 EPOXYPROPAN 1 Oi . ENHANCEMENT OF 1J HLOROBUIADIENE (MEETING ABSTRACT] (PP 78) MALIGNANT TRANSFORMATION Of A BABY KAMS1ER LUNG CELL LINE BY 2 C AND NEWBORN LUNG OF HAMSTER (MEETING A IN VITRO DNS AS AN ANALYTICAL 1001 FOR IN VITRO OEHCTION Of FECTS INOUCED BY VINYL CHLORIDE MONOMER (VCMl UNDER N RELATION TO ONCOGENIC POTENTIAL COMPARATIVE MALIGNANT TRANSFQRMAIION OF CELLS OF WHOLE EMBRYOS FETAL BRAIN MAMMALIAN ENZYME MEDIATED FORMATION OF REACTIVE MflABOUlfS MAMMALIAN METABOLIC ACTIVATION STUDIES lN VITRO AND IN VI MO MAMMALIAN METABOLISM OF VINYL CHLORIDE AND VINYLTDENE CHlOfiiOE I ON WITH C DEVELOPMENT OF HEPATIC ANGIOSARCOMA IN MAN INDUCEO BY VINYL CHLORIDE 1H0RQ1RAST AND ARSENIC COMPARE EFFECTS OF VINYL CHlORlOE IN MAN A CYIOGINEHC FOLLOW UP STUDY OCCUPATIONAL DISEASES IN CONNECTION WITH THE OF WORKERS EXPOSED TO VINYL CHLQRIOE MONOMER IN THE SS HISTORIES AMONG CHEMICAL WORKERS INVOLVED IN M MORTALITY STUDY Of WClfeERS IN THE N HUMAN flSSUES OF MONOMERS FOR Pi AG I Hi AND RUBBER MANUFACTURE OF PLASTIC B0T1LES FROM POLVVINH CHlORlOE MANUFACTURE Of POLYVINYL CHLORIDE IN GREAT BRITAIN MORTALITY MANUFACTURE OF POLYVINYL CHLORIDE *N EPiOEMlOiOGiC STUOt Of B MANUFACTURE OF VINYL CHlORlOE ANO IIS POLTMERS MANUFACTURE EVIDENCE FUR UlSILNCE I 61 66 133 267 138 10/ 203 285 269 100 49 111 43 140 119 24 190 113 140 195 54 80 30 129 70 69 64 73 55 108 16 Hi 44 46 51 50 67 28 47 29 /5 44 103 1// 104 25 117 112 53 6 38 19 40 115 31 68 124 59 123 39 35 37 ?5 98 109 125 41 4 120 87 66 6/ J 79 183 159 42 3) 83 h l.l DlCHlQftQETHVUNE HEPATOIOXIClTY PROPOSED IBlllTJES AND CLINICAL SIGNIFICANCE 1 ACTIVATION IMMUNOLOGICAL PP 731/ TISSUE Sy HUMAN RAT AND MOUSE LIVER CUPATIONAL INJURIES DUE TO INDUSTRIAL CHEMICALS , INDUSTRIAL VINYL CHLORIDE ITS IMPACT ON OCCUPATIONAL AN1AGONISM/ DAMAGE TO HEPATIC CELLULAR DOSE RESPONSE TDXICIlY DATA FOR CHEMICALS REQUIRING CED BY VINYL CHLORIDE MONOMER (VCM] UNDER MAMMALIAN T)t 1HE AGENIC EFFECT ON ELECTROPHILIC REACTIVITY OF THE/ COMBI NOCENICI TV Of VINYL CHLORIDE IN THE ABSENCE OF J51R!BUTTON Of **I4C VJNYICHIORIOE MONOMER (VC < TNG ABSTRACT) (PP |0>, POSSIBLE MUTAGENICITY Of CHIOROACETALDEHYDE, A POSSIBLE MUTAGENIC EFFECF ON ELECTROPHILIC REACTIVITY Of THE OF STRUCTURE ANO AC1IVITY/ RATS FOLLOWING ORAL ADMINS [RATION / SA1URABLE TISSUE DISTRIBUTION AND THE MU1AGENICITY AND CUV, SUBJECTS, MACROMOLECULAR BINDING ANO MUTAGENICITY ANO PREPARATION/ UPTAKE AND RATE OF MUlAGfNlCITY AND PURIFIED LIVER MICROSOMAL CYTOCHROME P 450 BY A ' CYIOCHROME P 450 AND THE STUDIES ON THE TO ONCOGENIC POTENTIAL^ COMPARATIVE MAMMALIAN DIFFERENCES IN INISIRAIlQN IN RAIS IMEE1ING ABS. DISPOSITION AND ND IN VITRO; SSESS THE KINETIC CONSTANTS OF 11 DICHlOROEIHYlENE MUNICATJON , TRICHLOROETHYLENE RELATIONSHIP OF Hit PURIFIED LIVER MICROSOMAL CYTOCHROME P45Q BY A OMPOUND VINYL CHLORIDE. STYRENE AND 1MEIH POSSIBLE GUANINE OF I CHlOROPROPENE ANO TWO Of ITS P0TEN1IAI MFOUNDS VINYL CHLORIDE. STYRENE ANO THEIR POSSIBLE VI TV NONVOLATILE METABOLITES AND NON CX1RAC1ABLE S1UDIES ON THE MUTAGENICITY Of VINYL CHLORIDE / VINYL CHLORIDE MUTAGENICITY VIA THE DETERMINATION OF TWO VINYL CHLORIDE CHROMATOGRAPHIC DETERMINATION Of TRICHLOROETHYLENE *4 ETHINOCYTIDINE MOIETIES IN RNA BY VINYL CHLORIDE L CHLORIDE ANO TRANSFORMATION TO PROTEIN ALKYLATING NOSINE ALKYLATION OF RNA BY VINYL CHlORlOE HE MUTATIONS AND GENE CONVERSIONS BY VINYL CHLOFtlOl NG PREPARATION (MEETING ABSTRACT.' DISTRIBUTION OF E tNBP> OR WITH SALMQ DETECTION OF ELECTROPHILIC TISSUES ALKYLATING ANO MUTAGENIC ICR05QMES COVALENT INTCRACIEON OF 2CHLORQETHANOL ANO CHLOROACEllC ACID. CONCEIVABLE DERIVATIVES Of NUCLEIC ACID BASES (ONA ANO RNAi BY THE CHEMISTRY AND BIOGENESIS OF THE S CONTAINING ETHENO DERIVATIVES OF NUCLEIC ACID BASES IN VIVO BY OMERS TO REGISTER TOTAL RADIOACTIVITY NON VOUTILE ER ENOOPIASMIC REUCULUM TARGET SITE OF HALOCARBON NT ON THE MUTAGENIC EFFECTIVENESS OF VINYL CHLORIDE Of MAMMALIAN ENZYME MEDIATEO FORMATION OF REACTIVE CTj ALKYLATION OF ONA ANO RNA BY HOTOMElRlC IDENTIFICATION OF CHLOROFORM HALOTHANF N OF I HI MUTAGENIC PROPERTIES Of VINYL CHLORIDE AND N VINYL CHlORlOE INDUCEO LIVER INJURY ANO 1UM0RS IN IAIES Of E1HYLENE DJHALIDES WITH PROTEIN AND DNA IN NENCES IN MElABDLlSU OF VINVLtDENE CHLORIDE BETWEEN INDUCTION Of STOMACH CANCER JN RATS AND INDUCTION OF PULMONARY ADENOMAS IN STRAIN A PULMONARY TUMORS INOUCED IN ALKUAHON OF DNA AND PROTEINS IN IBLE BINDING OF **I4C LABELLED lRlCHLOROETHYlENE TO THE IQAICm OF SOME HAlOMf IHANES IN MECHANISM FOR TRlCHLORQEEHVLENF HEPATOIOXIClTY (MEETING ABS1RACT MECHANISM Of ACTION AND DISTRIBUTION ANO BINDING OT a*14C RADIOA MECHANISMS IN CBL0RINA1E0 ALIPHATIC COMPOUNDS EXPERIMENTAL FOSS MECHANISMS IN THE PATHOGENESIS OF VINYL CHLORIDE OISEASE MEDIATED MUTAGENICITY AND CARCINOGENESIS (MEETING ABSTRAC1) | MEDIATED MUTAGENICITY OF VINYL CHLORIDE IN S TYPHIMURIUM STRAIN MEDICAL SURVEILLANCE SYSTEM EOR NEQPlASUC AND NON NEOPLASTIC DC MEDICINE ANO VINYL CHLORIDE MEDICINE PRACTICE IN IRAN MEMBRANES BY CHLORINATED OLEFINS WITH EMPHASIS ON SYNERGISM ANO METABOLIC ACTIVATION EXAMPLE VINYL CHlORlOE RESOLUTION Of METABOLIC ACTIVATION STUDIES IN VITRO AND IN VIVO < EVALUATION METABOLIC ACTIVATION Of VINYL CHLORIDE IN VITRO (MEEIkNC AfiSIRAC METABOLIC ACTIVATION Of CHLORINATED ETHYUNIS DEPENDENCE Of MUT METABOLIC ACllVAllQN / THE NON MUTAGENICHY AND R[ MEEABOUC APPROACH 10 INDUSTRIAL POISONING BLOOD KINEllCS AND 0 METABOLIC INTERACTION BETWEEN SlYRENE AND ORGANIC SOLVENTS (IAEEI MEIASOMC PR0DUC1 Of l 2 DICHLOROCTHANE (F1HYLENE DlOftDHlOEj C MEEABOLICALLY FORMED EPOXIOE5 / METABOLIC ACTIVATION Of CHLORIN HL1ABOIISM AND MUTAGENICITY OF HAlQGENAUQ OllfINS A COMPARISON METABOLISM ANO PHARMACOMNEIIC PROFILE OF VINYllDCNE CHLORIDE IN ME1ABOLISM AND THE ACUTE TOXICITY Of I 1 OICHlOROElHUEKE METABOLISM OF I 2 OIBROMOEIHANE (UEEElNG ABSTRACT) ME I ABOLISH Qf 1 2 DlCHlQROEIHANL (MEETING ABSTRACT I METABOLISM OF CHlORlNATFD ALKENES AND ALKANES AS RllATEO TO TUAI ME 1ABOLISH OF HALOGENAUD [lHYUNES METABOLISM QF THE CARCINOGEN I 2 OIBROMOEIHANE MEIABOLISH OF TRICHLORQEIHYLENE AND TETRACHLOROETHYLENE IN HUMAN METABOLISM Of TRJCHlQROETHYLENE BY THE ISOLATED PERFUSEO LUNG METABOLISM OF VINYL CHLORIDE MEIAB01ISM Of VINYL CHLORIDE BY THE ISOLATED PERFUSED RAi LIuER MEIAfiOlISM OF YJNYi CHLORIDE AND RELATED COMPOUNDS METABOLISM OF VINYL CHLORIDE DESTRUCTION Of THE HEML Of HIGHLY METABOLISM Of VINYL CHLORIDE ME IABCi ISM OF VINYL CHLORIDE METABOLISM Of VINYL CHLORIDE AND VI NYU DENE CHLORIDE IN RELATION METABOLISM OF VINVUOENE CHLORIDE BElWtEN MICE AND RAJS metabolism of **i4c j i dichloroethylene after single ORAt adm MElABQUSM Of 14C AND 36CL LABElEO VINYL CHlORlOE IN VIVO A METABOLISM Ihf USE Of iNHAiAhON TECHNiOUiS Tu A METABOLITE LEVELS TO ATMOSPHERIC CONCENTRATIONS PRELIMINARY COM METABOLITE M11A60USM Of VINYL CHLORIDE DESIRUINUN OF M rt METABOLITES (MEETING ABSIRACT, iPP t/5 176' MUtAGENlUPr 0 METABOLITES iMEEHNG ABSIRACJi CARCINOGENICITY AND CHEMMi R METABOLITES (MEETING ABSTRACT METABOLITES (MEETING ABSTRACTi MUTACENlCllf Of INOtfSffttt CD IhREE SUP AU[ORADiQCRAPHr Of METABOLITES AND RELATED COMPOONOS (MEETINC ABSIRAOj (PP 19/ METABOLITESCHlCRQOXIRANE ANDCHLOftUAtEIAiOEHVOE MONOMER HYDRATE METABOLITES IN URINE (MEETING ABSTRACT i METABOLITES IN URINE GAS LIQUID METABOLITES IN VITRO AND IN VIVO FORMATION Of 3li* METABOLITES IN VITRO LIVER MICROSOMAL UP1AH1 Of **J4C '.tNr METABOLITES JN VITRO AND IN VIVO FORMA 11 UN OF J N**& ((HfNO AOE METABOLITES IN YEAST INDUCH0N Of Cl MEIAB0LITES Of BENZOiAjpYRENE IN IHE IS0LAIE0 PERFUSED RABBH lU MEIA80LIIES OF HAlQGENAlEP OLEHNS WIN i .4 NJfROBEN/Yl iPYPtDiN METABOLITES OT HALOGENATED OLEflhS PRODUCED Bi HUMAN AND AhiMAi MtlAflOlHFS OF THE CARCINOGEN IRICHLOROfTHVL ENf IN RAI Hf PAT 1C U METABOLI1ES OF VINYL CHLORIDE EhF MUTAGENICITY Of CHLOPGElKYL ME1ABOUUS OF VINYL CHLURlDl IN UlvD AND HI VITRO (MEETING ABST METABOLITES OF VINTL CHlORlOE IN RAIS ME1ABOLITES OF VINYL CHlORlOE TMEEIlNG ABSTRACIj FORMATION Of METABOLITES ANO NON EXTRACTABLE MEIABOM1ES <MEETING ABSTRACT! MEIA601ITES MFIABOLITES Uy LUMMf METABOLITES MUlAGENlClEYANO CHROMOSOMALABERRATIONS AS Ah AH METABQ1IMES Of VINYL CHLORIDE AND VINYL BROMIDE iMEEHNG AflSFRA METHQXYFLURANE TETRACHLOROErHYLENE ANO IfllCHlOROElHYiENF METHYL CHLORIDE A COMPAJUSfl MICE rMEETING ABSTRACI) SOME ASPECES ON DOS! KfSPONSt 1 MICE ANO RATS TISSUES INVIIRO I MEETINGABSTRACT INTERACTION MICE ANO NATS luffc MICE BY HAIOGENATEO AliPHAIIC FUMIGANTS MICE BY SUBSTITUTED ORGXNOHAUUES MICE BY VINYL CHlORlOE MONOMER MICE EXPOSED TO VINYL CHlOfiiOE MICE LIVER CONSTITUENT IN vJVU AND IN VilPU MICE IRREVERS UCC 106400 2 50 1/5 >40 44 45 l?\ ib b! 60 19 56 is5b9 3 34 58 .1]68 198 J5J ?4? ?U n ;4 in >0 2ib 248 286 il? 214 202 362 90 159 212 2 ft ?J2 237 216 ?2S 211 212 202 2)4 209 208 229 210 2)1 221 Hi 10? 110 196 226 116 ] 44 136 135 199 325 18? 296 303 30? 130 122 J 40 J09 121 191 I2B 12? 01 ETHYLENE DiBRGMpDF DURING GFSEATION BY RATS AND Nr{ CHLORIDE OOMINANI LElHAL STUDIES IN MAlC CD 1 f FHEHS ANO Ql J 4 plCHlOROBUTENC 2 IN O'HA SWISS FLORID! PRQOUCElON WORKERS EXAMINATION BY IN VIVO [ DESTRUCTION Of THE HEME OF HIGHLY PURIFIED UVER The IftlERACriOfl OF VlrtYl CHLORIDE WITH RAT HEPATIC NVJflONMENTAl CHEMICALS ON THE GENETIC REGULATION Of E TRICHIOR0ETHUENE HEPATOIOXFCITV AND INDUCNON OF DiNG OF THE CARCINOGEN TRICHLOROETHYLENE EG HEPATIC iDFNCE FOR 2? J TRICHLORO OAlRANE FORMATION DURING 0 PROTEIN ALKYLATING METABOLITES IN VtIRO LIVER UETHVLENE IQ CELLULAR MACROMOl [COLES iMEETING VARIOUS CHEMICALS iMEtllNC ABSTRACT) Of THE CARCINOGEN IRlCHLOROETHTlENE IN RA1 HEPATIC N2IDINE ANO VINYL CHLORIDE EVALUATED IN LABORATORY VINYL CHLORIOE carcinogenicity an experimental .0 FORMATION OF 3N**4 ETHENOCVTlDME AND OTHER CHLORINATED UHVlENES (MEETING ABSTRAC CENIC POTENTIAL OF CHLORINATED ETHYLENES TENTATIVE L OF SPQNIANEQUS ABORTIONS POWER IN ENVIRONMENTAL INDUSTRIAL LESiONS ASSOCIATED WllH EXPOSURE TO VINYL CHLORIDE jO KiNEElCS ANO OISIRJBUHON OF **I4C VINVlCHLORIDE nKki 'AETHOO FOR THE MEASUREMENT Of VINYL CHLORIDE NARY STUDIES Of THE FATE OF INHALCO VINYL CHLORIOE ON Of THE GENETIC EffECIS INDUCED BY VINYL CHLORIDE PHARMACODYNAMICS AND UPTAKE OF VINYL CHLORIDE CHLORIDE IMPACT Of THE LINK BETWEEN VINYL CHLORIOE AGE (N MEN OCCUPATIONALLY UPOSEO 10 VINYL CHLORIDE THE VINYL CHLORIDE HE METABOLITES CHLOROOXlRANC ANO CHLOROACF1ALOE HYDE il( EXPERIENCE OE WORKERS EXPOSED TO VINYL CHLORIDE MORTALITY EXPERIENCE Of WORKERS IN A VINYL CHLORIDE PORTAL HYPERTENSION IN VINYL CHLORIDE ESiS IN RECtNERATIN I HE EFFECT OF VINYL CHlORlOC pulmonary tumors induced in mice dy vinyl chloride EVIDENCE FOR EXISTENCE IN HUMAN TISSUES OF UP AUIQRADIOCRAFHY Of ORGANIC SOLVINJS ANO PLASTIC MORIAH TY ANO CANCER ORKERS. CLINICAL AND iNTUllCAHON RKIRS FOLLOWING CHRONIC EXPOSURE TO VINYL CHLORIDE cn PROPORTIONAL PRODUCTION WORKERS/ UCIlON PLANT ft IN THE MANUFACTURE Of POLYVINYL CHLORIDE IN GR/ URIDE WORKERS ' IN A CONTROLLED INDUSTRIAL ENVIRONMENT A LONG TERM AND ITS POLYMERS. ASSOCIATIONS Of CANCER HOftl STUDY ON TRICHLOROETHYLENE EXPOSURE ANO CANCER i DlBHOMOPflOPYL iPHOSPHAlE TEIRAKlSlHYDROXYMElHY/ URIUM S1RA1NS/ HUMAN RAT AND CHLORIDE ANO IES COMPARISON WITH A KNOWN ALKYLATING iMEEIIHG ABS1RACII HICROSOME MEOIAEEO CHLOROPRENE OBSERVATIONS OF CARCINOGENESIS ANO flNG SYSTEM {MEETING A/ VINYL CHLORIOE DEPENDENT THE INFLUENCE OF CONTAMINANTS ON THE DRINKING WATER i 1HE ETING ABSTRACT)/ STRUCTURAL CORRELATIONS OF CARCINOGENIC ANO FINS/ CARCINOGENIC POIENTIAl HALOGENATEO INDUSTRIAL CARCINOGENIC AND POTENTIAL HAtOGENATED INDUSTRIAL CARCINOGENIC AND II ACTIVATION Or THE ISOLATED PERFUSED FLA/ THE J ACTlVAltON THROUGH CONJUGATION WITH GLUT/ THE AC1IVA1I0N OF CHlORINAUO ETHYLENES DEPENDENCE OF COMMENT ON THE MEETING ABSIRACE)/ SI RACK. ALKYTAIING ANO ES OT BEN2ENC AND I 2 0ICHL0R0E1HANE / tin ANO WITHOUT PSf TREATMENT WllH SCOlUM PHENOBA/ MICE INHALATION MICE V MICE CARCINOGENIC ACTIVITY OF Dl AND TfttfUHUlONAL ALPHA C MICROSCOPY CAPILLARY ABNORMAL I DCS IN POLYVINYL MICROSOMAL CYTOCHROME P *90 BY A METABOLITE METABOLISM Of VlN MICROSOMAL CYTOCHROME P 450 IN VITRO MICROSOMAL ENZYME SYSTEMS MICROSOMAL EN2VMES (MEETENC ABSTRACll EFTEC1S Of ' ACUT MICROSOMAL PROTEINS AND TO EXOGENOUS DNA IN VITRO C0VALEN1 Bl MICROSOMAL TRICHLOROETHYLENE QXIDA1IQN [MEE1ING A6STRACII SPE MICROSOMAL UPTAKE Of *M4C VINYL CHLORIDE AND TRANSFORMATION T MICROSOME DEPENDENT COVALENT BINDING Of THE CARCINOGEN TRtCHlQR MiCROSOMi MEDIATED MUTAGENESIS OF A CHINESE HAMSTER CELL LINE BY MICROSOMES COVALENT INTERACTION OE METABOLITES MIGRATION Of VINYL CHLORIDE FROM FVC PACAAClNGS MODEL ECOSYSTEMS THE ENVIRONMENTAL FATE OF THREE CARCINOGENS MODEL FOR CARCINOGENESIS STUDIES |PP 119 1461 MOIETIES IN RMA BY VINVl CHLORIDE ME1AB0U1ES IN VITRO ANO IN VI MOLECULAR ASPECTS 10 THE OAlRANE FORMATION Of TRICHlOROEIHtlENE MOLECULAR RULES {PP 121375) CARClNO MONITORING SURVEILLAN MONITORING A CYTOGENETIC APPROACH MONOMER IMEETING ABSIRACTl HlSTOPATHOlOCY OT LIVER MONOMER fVCM) (MEETING ABSTRACll MtlABOLlC APPROACH TO IND MONOMER IVCM1 IN AIR A PRA MONOMER IVCM) IN RATS PRELIM MONOMER (VCMJ UNDER MAMMALIAN METABOLIC ACTIVATION STUDIES IN V MONOMER ADMINISTERED BY VARIOUS ROUTES TO RATS MONOMER ANO CANCER * PRODUCTION AND USE OF VINYL MONOMER ANO OTHER CHEMICALS , CHROMOSOMAL DAM MONOMER HEALTH PROBLEM MONOMER HYDRATE , VINYL CHLORIDE MUTAGENICITY VIA T HOHOMIR IN THE MANUFACTURE Of POLYVINYL CHLORIOE IN GREAI BRlTAI MONOMER PRODUCTION PLANT MONOMER WORKERS A HEMODYNAMIC SlUOV MONOMER CHlOROETHYLEKE OXIDE ANO CHLOftACE[ALDEHYDE ON DNASYH1H MONOMER MONOMERS FOR PLASTICS AND RUBBER MANUFACTURE MONOMERS 10 REGISTER TOTAL RADIOACTIVITY NONVOLATILE MEIABOUT MORBIDITY IN A CROUP OF SWEDISH VCH ANO PVC PROOUC1JON WORKERS MORPHOLOGIC FEATURES Of HEPATIC ANGIOSARCOMA IN VINVL CHLORIDE W MORPHOLOGICAL CHANGES OF THE LIVER AflER CHRONIC VINYL CHLORIOE MORPHOLOGICAL DESCRIPTION OF THE LESIONS / HEPATIC ANGIOSARCOMA MORTALITY AMONG EMPLOYEES Of PVC FABRICATORS MORTALITY AMONG LAUNDRY AND DRY CLEANING WORKERS {MEETING A8STRA MORTALITY AMONG VINYL CHLORIDE WORKERS MORTALITY AW CANCER MORBIDITY IN A GROUP OF SWEDISH VCM ANO PVC MORTALITY EXPERIENCE OF WORKERS IN A VINYL QUURIQE MONOMER PROO MORTALITY EXPERIENCE OF WORKERS UPOSEO 10 VINYL CHLORIOE MONQME MORTALITY EXPERIENCE OF A COHORT OF VINYL CHLORIDE POLYVINYL CHI MORTALITY EXPERIENCE IN 594 EMPLOYEES VINYL CHLORIOE EXPOSURE MORIALITY OF WORKERS EXPOSED TO CHIQROPRPNE MORTALITY STUDY Of WORKERS IN THE MANUFACTURE OF VINYL CHLORIDE MORTALITY WITH HAL0ME1 HANES IN DRINKING WATER M0R1ALITV f A CD MOUSE SHIN CARCINOGENICITY TESTS OF THE FLAME R{TAROANTS TRlStf MOUSE LIVER MEDIATED MUlAGENIClTV OF VINYL CHLORIDE IN S TVPHIM MUTAGEN {PP 505 519) / THE MUTAGENICITY Of THE CARCINOGEN VIH MUTAGENESIS OF A CHINESE HAMSTER CELL LINE BY VARIOUS CHEMICALS MUTACENESJS (PP ?05?1?| MUTAGENESIS EFFECTS Of LIVER EXTRACTS AND A FREE RADICAL GENERA MUTAGENIC ACTIVITY Of DIBROMOCHlQROPRQPANE (QBCPi MUTAGENIC ACTIVITY Of HALOGENATEO COMPOUNDS FOUND IN CHLORINATED MUTAGENIC ACTIVITY Of CHEMICALS lOENILEO IN DRINKING WATER {ME . MUTAGENIC ALKYL HALIDES (PP 163 9 71) MUTAGENIC AND CARCINOGENIC RISKS ASSOCIATED WllH HALOGENATEO OLE MUTAGENIC AND CARCINOGENIC EFFECTS Of VINYL CHLORIOE MUTAGENIC ANO TERATOGENIC RISKS ASSOCIATED WITH VINYL CHLORIDE MUTAGENIC CHEMICALS II HALOGENATEO SATURATED HYDROCARBONS MUTAGENIC CHEMICALS I HALOGENATEO UNSATURAIEO HYDROCARBONS MUTAGENIC EFFECT OF 1 2 DICHLOflOfTHANE ON SALMONELLA TYPHIMURIUM MUTAGENIC EFFECT OF l 2 QICHLQROETHANE ON SALMONELLA TYPHIMURIUM MU1AGENIC EFFECT ON ELECTROPHILIC REACTIVITY OE THE ME1AB0UCALL MUTAGENIC EFFECTIVENESS Qf VINYL CHLORIOE METABOLITES MUTAGENIC EFFECIS OF VINYL CHLORIOE IN DROSOPHILA MELANOCASILR ( MUTAGENIC EFFECTS OF ALLYL ANO AUYLGGCNIC COMPOUNDS (MUTING A& MUTAGENIC EFFECTS OF PEIROL IN DROSOPHILA MELANQCASTER I EFEFC MUTAGENIC EFFECTS OF VINYL CHLORIDE ON DROSOPHILA MEIANQGA5KH W J>4 ND ANIMAL (fSiUES ALKYLATING AND MUTAGENIC METABOLITES Of HALOGENAIED 01 EFlNS PRODUCED BY HUMAN A Hit A COMPARISON 01 THE MUTAGENIC PROPERTIES Of VINYL CHlOHlOE ANO MLfHYL CHLORIOE N fACUJllES ONCOGENIC ANO MUTAGENIC RISKS IT! COMMUNITIES WITH POLYVINYL CHLORIDE PRODUCED 323 l CHLORIOE ANO uNYL 6EN2LNE (STYRENE) METABOLISM MUTACENicm AND CARUNOCIMCHV viny 139 TISSUE MEDIATED MUTAGENICITY AND CARCINOCTNESIS i MEETING ABSTRACT I iPP /3j 125 OR IN VIFRO DETCCliON OF MAMMALIAN ENZYME MEDIAE MOlAGENlCm AND CHROMOSOMAL ABERRATIONS AS AN ANALYTICAL TOOL f 137 [HE MU1AGENICIEY ANO DNA MODIFYING ffFFCT OF HALOALKANES 108 MUTAGENIClIt AND M11AB0USM OE YlNYt CHLORIDE 129 ACTj THE MUTAGENICITY AND MEIABClISM Of 1 ? OlCHLOHOEJHANf /MEETING AflSJS 114 NOS MUTAGENICITY AND MEIABOLISM OF VlNU CHLORIDE ANO RELATED COMPOU L39 lPP THE PREDlCltVE vAtUE Of TISSUE ME0IA1ED MUTAGENICITY ASSAYS TO ASSESS THE CARCINOGENIC RISK OF CHEMICALS 113 Of L 2 DICHLORQETHANE (ETHYLENE DICHLORIDEl CH/ MUTAGENIClIt OF CHLOROACEIALDEHYOE A POSSIBLE MilABOtlC PRODUCT L23 ROETHANOL AND CHtOROACElIC ACID CONCEIVABLE1 THE MUTAGENICITY OF CHlORGE FHYL LNE OXIDE CHLOROACEIALDEHYOE 2 CriLQ 200 IOES IN SALMONELLA ANO STREPTGMYCES MUTAGENICITY OF DICHLORVOS AND OTHER blfiUClURAllY RELATED PESTlC 141 MUTAGENICITY OF HALOGENATEO ALKANES ANO THflfi OERlVAElvES 195 ND ACTlVilYMETABOLISM AND MUTAGENICITY Of HA10GENAIED OLEFINS A COMPARISON OF SIRUCIORE A 134 GtNEltC SYSTEMS (MEETING ABSTRACll. MU1AGFNICIIY OF INDUSTRIAL COMPOUNDS EVA1UAIED BY MEANS Of YEASI 104 0 THEiR POSSIBLE METABOLITES IMUTING ABSIRACTl MUTAGENICITY OF INOUSIRIAL COMPOUNDS VINYL CHIORIDE SfYRENE AN 103 THELR POSSIBLE MEIABGWTES (MEETING ABSTRACll' MUTAGENICITY OF INDUSTRIAL COMPOUND VINYL CHLORIDE STYRENE AND 2 45 MUTAGENICITY OF PESTICIDES CONTAINING I 3 OlCHtOHOPROPENE 110 WITH A KNOWN ALKYLATING MUTAGEN (PP 505 r THE MUTAGENICITY OF 1HE CARCINOGEN VINYL CHLORIDE AND ITS COMPARISON 131 L53S (ISSUE MEDIATED MUTAGENICITY QF VINYLIDENE CHLORIDE IN SALMONELLA IVPHIMUftlUM TA 244 ERSONS WITH AND WI2H0UI VC ILLNESS AND ON VC EA MUTAGENICITY QF VINYL CHLORIDE EXIERNAE CHROMOSOME STUDIES ON P 117 iMEETING ABSIRACTl '.PP 192 1 STUDIES ON 1HE MUTAGENIClIt OF VINYL CHLORIDE METABOLFIES AND RELATED COMPOUNDS 107 HUMAN RAT AND MOUSE LIVER MEDIATED MOTAGtHlCm OF VINYL CHIORIDE IN S TYPHIMURIUM STRAINb 132 MUTAGENICITY OF VQLAHIE ANESTHEHCS I MELTING ABSIRACTl 316 S (MEETING ABSIRACn' THE MUTAGENICITY OF WASTE PR0DUC1S FROM THE VINYL CHLORIDE JNDUS1RIE 329 MUIAGENICITY TESTS IN CHEMICAL CARCINOGENESIS /PP 229 2401 112 EHYOE MONOMER HYDRATE VINYL CHLORIDE MUIACCNICIIY VIA THE METABOLITES CHLOROOXIRANE AND CHLOROACETALD 322 FIVES INDUSTRIAL MUTAGENS AND POTENTIAL MUTAGENS I HALOGENAIEO ALIPHATIC DEXIVA 322 INDUSTRIAL MUFAGENS ANO POTENTIAL MUTAGENS I HALOGENATEO ALIPHATIC DERIVATIVES 326 CLES ANO OPTIONS (N VITRO TESTING OE ENVIRONMENTAL MUIAGENS/CARCINOCENS i PP 27 33) ' SESSION II CARCINOGEN SCREE 115 YEAST INDUCTION 01 GENE MUTATIONS AND GENE CONVERSIONS BY VINVl CHLORIDE MEIABOUTES IN >94 DRINKING WATER AND HEALTH RECOMMENDATIONS OT THE NATIONAL ACADEMY OF SCIENCES 68 ALOGENATEO OLEFINS WllH 4 (4 NkTROBENZYOPYRIOINE I nbpi OR with Salmonella typhimurium (meeting abstract) / ddeci 203 (RIAL CHEMICALS MEDICAL SURVEILLANCE SYSTEM (OR NEOPLASTIC AND NON NEQPLAS1IC OCCUPAIlONAL INJURIES DUE TO INDUS 330 OCCUPATIONAL CANCER DISCUSSED AT NEW YORK CONFERENCE 153 RMAT10N OF CELLS Of WHOLE EMBRYOS FETAL BRAIN ANO NEWBORN LUNG OF HAMSTER {ME El INC ABSERACT) IN VITRO MALIGHANl 202 RIDE POLYVINYL CHLORIOE WORKERS 1977 UPDATE OF THE NIOSH REGISTER ANGIOSARCOMA Of IH[ LIVER IN VINYL CHID 25 TOTAL RAOlQAClIVITY NONVOLATILE MU ABOUTES AND NON EXTRACTABLE MElABQlllES (MEETING ABSTRACll , THREE STEP AUT 119 ABSENCE Of ME1AB0UC ACTIVATION lHf NON MDTAGENICUT ANO RECOMBlNOGINiCIIT Of VINYL CHIORIDE IN I HE 201 MEDICAL SURVEILLANCE SYSTEM TOR NEOPLASTIC AND NON NEOPLASTIC OCCUPATIONAL iNlUfllfS DUE TO INDUSIRIAL CHEMICALS 91 SPh VINVL CHLORIDE INDUCED DEPRESSION OF HEPATIC NON PROTEIN SULFHYORVl CONIENT AND EFFECTS ON BROMOSULPHALElN (B 29 0 PLASTIC MONOMERS 10 REGISTER TOTAL RAOIOACTTVFIY NONVOLATILE METABOLITES ANO NON EXTRACTABLE METABOLITES {Mffll 39 E IN VIVO A FORMATION OF IMIDA20L DERIVATIVES OF NUCLEIC ACID BASES (ONA AND RNAI BY METABOLITES OF VINYL CHLOttlU 37 TING ABSTRACTr FORMATION OT UHENO DERIVATIVES OF NUCLEIC ACID BASES IN VIVO BY METABOLIIES OF VINYL CHLORIDE (MEE 18 ENF BINDING OF ,-[4C VINYL CHLORIDE TO PROFEINS AND NUCLEIC ACIDS IN VITRO AND IN VIVO (MEETING ABSTRACT) ( 03VAL 9 ftEACIIOHS Of EPOXY 1 t 2 IRICHLOROETHANE WITH NUCLEOPHILES 241 SOR LESIONS IN EXPOSED POPULAIIONS AS INDICATORS OF OCCUPAIlONAL CANCER RISK , PRECUft 330 OCCUPATIONAL CANCER DISCUSSED AT NEW YORK CONFERENCE 338 PERSPECTIVES iPP 127149). OCCUPATIONAL CHEMICAL CARCINOGENESIS NEW fAUS PfllQfttlKS ANO 2&* TIC B01TIESFROM POLYVINYL CHLORIOE OCCUPAIlONAL DISEASES IN CONNECTION WITH THE MANUFACTURE Of PLAS 203 RVESLLANCE SYSTEM FOR NEOPLASFlC ANO NON NEOPLASTIC OCCUPAIlONAL INJURIES DUE 10 INDUSIRIAL CHEMICALS (MEETING ABSER 334 OCCUPATIONAL LUNG CANCER (PP 25 511 269 VINYL CHLORIDE 11$ IMPACT ON OCCUPATIONAL MEDICINE PRACTICE IN IRAN 272 BSIAHCE.S / OCCUPATIONAL SAFETY AND HEALTH STANDARDS FOXIC AND HAZARDOUS SU 248 CALS, CHROMOSOMAL DAMAGE IN MEN OCCUPATIONALLY EXPOSED 10 VINYL CHLORIOE MONOMER ANO OTHER CKlMI T6T INHALED I 2 DlBROHOUHANE UNDER THE INFLUENCE OF/ OCCURRENCE OF HEPATOCELLULAR TUMORS AND HEMANGIOSAtiCOMA EN RATS M VOLATILE CARCINOGENS 0CCUR8FNCE. FQRMAHON AND ANALYSIS (PP 1943 19491 124 ALKYLATING AND MUTAGENIC METABOLITES OF HAlOGEKATED OLEFINS PRODUCED BY HOMAN AND ANIMAL IlSSUCS , 69 ITCTION Of ELECTROPHILIC METABOLITES OF HALOGENATEO OLEFINS WITH 414 NJTPOBEN/YDPYRIDlNE iNBPj OR WITH SALMONELLA 100 DAMAGE IQ HEPATIC CELLULAR MEMBRANES BY CHLORINATED OLEFINS WITH EMPHASIS ON SYNERGISM AND ANTAGONISM 197 AME1ERS ASSOCIATED WITH CARCINOGENESIS (HALOCENAIEO OLEFINS VINYL ANO ALLYL ANALOGS AND EPOXIOESI (PP UU 595 METABOLISM AND MUTAGENICITY OF HAL0GEHA1ED OLEFINS A COMPARISON OF STRUCTURE AND ACIIVI1V 324 CENT FINDINGS ON THE CARCINOGENICITY OF CHLORINATED OLEFINS , RL 325 AND CARCINOGENIC RISKS ASSOCIATED WITH HALOGENATEO OLEFINS / MUTAGENIC 255 RIDE PRODUCTION FACILITIES/ ONCOGENIC AND MUTAGENIC RISKS IN COMMUNITIES WllH POLYVINYL CHLO 54 NYL CHLORIDE ANO VTNYL1DENE CHLORIDE IN RELATION TO ONCOGENIC POTENTIAL / COMPARATIVE MAMMALIAN METABOLISM Of VI 157 OE ONCOGENIC RESPONSE OF RAT SKIN LUNGS. AND BONES 10 VINYL CHLORI 23 OF VINYL CHLORIDE ANO ITS POSSIBLE RELATIONSHIP TO ONCOGENICITY IN RATS ' DOSE OEPEHDENI FAIE 29 6IXISM Of *I4C 2 I OICHLOROE1HYLENE AFTER SINGLE ORAL ADMINISTRATION IN RATS (MELTING ABSTRACT) / DISPOSITION AN LS FATE OF **14 CVINYL CHLORIOE AFTER SINGLE ORAL AOMINlSTRAItON IN RAIS 54 1C PROFILE OF VINYLIDENE CHLORIOE IN RAES FOLLOWING ORAL AOMJNSTRAllOH . METABOLISM ANO PHARMACQKlNE! IBS AGE ANO FASTING/ ORAL TOXICITY QF I I OICHlOROETHYLENE IN FHE RAT EFFECTS OF SFX 190 POSSIBLE METABOLIC INTERACEION BETWEEN STYRENE AND ORGANIC SOLVENTS {MEEfINC ABSTRACT) (PP 10) 25 ivm NON YOlAlIl/ THREE S1EP AUTORADlOCRAPIlr OF ORGANIC SOLVENTS AND PLASTIC MONOMERS TO REGISTER TOTAL RAOIOACT |63 PULMONARY ADENOMAS IN STRAIN A MICE BY SUBSTITUTED ORGANOHAllDES 1 INUUCUUN U( 60 IRANI fORMAtIQN DURING MICROSOMAL 1RICHLUR0EIHYLENE OXIDATION (MEUING ABSLNACIf : SPECTRAL LVIOENCl FOR 223INIC UCO 106401 90 m \% 94 16 44 41 44 2B0 3 267 166 297 55 31 16 130 nt 146 m m 20 64 322 n 62 26 21 66 84 122 14? 147 264 26 210 26 147 -H 29* 21? 264 274 210 178 93 ?|4 77 220 ] 47 266 240 267 260 284 268 241 262 263 331 241 JJ3 1J9 H8 266 *6 *4 1*9 122 *1 22? 275 266 29b 202 260 217 HE EfFECI Of mil CHLOfllOi MQWGMER CHLOROETHYlENl OXIDE AND CHLORACETAlDEHVDE ON DNA SYNTHESIS IN REGENERATING RAT . CONCEIVABLE/ THE MUTAGENICITY OF CHlOROETHYLENE OXIOE. CHLOROACETAIDEHYOE ? CHIOROETHANOL AND CHLOROACEHC ACID YLENES (MEETING ABSIRAC/ MOllCULAfl ASPECTS FO THE OXIRANE FORMATION OF TRICHLOROETHYLENE AND OTHER CHLORINAEEO EM RlCHlOROETHYLENE INDUCED DEACTIVAllQN OF CYTOCHROME P 460 AND LOSS Of LIVER GLUTATHIONE IN VIVO /T CY10CHRQMS P460 AHO THE MllABOlISM OF VINYL CHLORIDE HEME OF HIGHLY PURIFIED LIVER MICROSOMAL CYTOCHROME P 460 BY A METABOLITE / MlABOllSM OF VINYL CHLORIDE DESTROUI VINYL CHLORIDE MEOIA1ED CYTOCHROME P 440 DESTRUCTION (MEETING ABSTRACT', HVL CHLORIOE WITH RAT HEPATIC MICROSOMAL CYTOCHROME P 460 IN VITRO AL HEALTH HA2ARD OF SUBSTANCES LEACHED FROM PLASTIC PACKAGING / THE INTERACTION OF VI / THE POFENFI MIGRATION OF W4YL CHLORIDE FROM PVC PACKAGINCS IMMUNOLOGICAL MICHMISMS IN THE PATHOGENESIS OF VINYL CHLORIDE DISEASE T ETRACHLGRQE1HYlEN{ ( PERCHLQAOC THTUME > ENT INTELLIGENCE BULLETIN 2Q lflftACHLOROElHYUNE ( PERCHLOflOEIHYltNfl / CURB METABOLISM OF TRICHLOROETHYLENE BY THE ISOLATED PERFUSED LUNG ON OT METABOLITES OF BCN20(AIPTREHC IN (HE ISOLATED PUFUSID RABBil LUNG PREPARATION (MEETING ABSTRACT! , DlSIRkBUl ATE OF METABOLISM OF VINYL CHLORIOE BY THE ISOLATED PERFUSED RAF LIVER PREPARATION / UPTAKE AND R MONILIA TYPHIMURMJK It ACTIVATION BY THE ISOLATED PERFUSED RA1 LIVER THE MUTAGENIC EfFECI OF I 2 DICHLOROE THANE SPERM COUNT DEPRESSION IN PESlJClOE RPPLICA10RS EXPOSED FO DIBROMQCHLQROPROPANE MU1AGEN1CIIY Of PESTICIDES CONTAINING 1 3 DlCHLCROPBCPENE NlCIIV OF OICHLORVOS AND OTHER STRUCTURALLY RELATED PESTICIDES JN SALMONELLA AND STREPTOMYCES ' MUTAGE DICHLOROEMANE/ MUIAGENlC EFFEC1S OF PETROL IN DROSOPHILA MELANOGAS1ER I EFFECTS OF BENZENE AND L 2 ED BY VARIOUS ROUTES TO RATS/ PHARMACODYNAMICS AND UPIAKE OF VINYL CHLORIDE MONOMER ADMINIS1ER ORAL A0M LUSTRATION >' METABOLISM AND PHARMACOKINETIC PROFILE OF ViNYllOENE CHLORIDE JN RATS FOLLOWING WIDALS <PP W m RELEVANCE OF OOSC DCPiNOENT PHARMACOKINETICS IN THE ASSESSMENT OF CARCINOGENIC HAZARD OF CNF PHARMACOKINETICS OF VINYL CHLORIDE IN THE RAT PHARMACOKINETICS Of VINYL CHLORIDE PHARMACOKINETICS Of VINYUDENE CHLORIDE fN THE RAT PHARMACOKINETICS Of HALOGENAFEO ElHYtENES (MEETING ABSTRACT} 1C INJURY BY VINYL CHLORIOE IN RAIS PRE1RLATED WITH PHENOSARBllAl , ACUTE HEPAT OF VINYL CHLORIDE EXPOSURES TO RATS PRE1RCA1EO WIIH PHENOBARBI1AL EFFECTS ANOGASTER WL1H AND WITHOUT PRETREAFMENT WITH SODIUM PHENOBARBtTURATE i MUTAGENIC EFFECTS OF VINYL CHLORIDE ON DROSO ICAL EFFECTS OF ALPHA BENZENE HEXACHLORIOE DIBUTYL PHTHALATE AND TRICHLOROETHYLENE ON SACCHAROMYCES CiREVlSIAE SIRA MEETING ABSTRACT!/ A TERATOLOGIC EVALUATION OF PLASMA SOLUBLE EXTRACTS OF POLYVINYL CHLORIOE PLASTICS IN RATS i ONAl DISEASES IN CONNECTION HYI1H THE MANUFACTURE OF PLASTIC BOTTLES FROM POLYVINYL CHLORIDE 1 OCCUPATI THREE STEP AUTORADIOGRAPHY OF ORGANIC SOLVENTS AND PLASTIC MONOMERS TO REGISTER 101*1 RADIOACTIVITY. NONVOLATILE M POTENTIAL HEALTH HAZARD OF SUBSTANCES LEACHED FROM PLASTIC PACKAGING / THE ENCE EOR EXISTENCE IN HUMAN USSUES OF MONOMERS FOR PLASTICS AND RUBBER MANUFACTURE EVlD ON OF PLASMA SOLUBLE EXTRACTS OF POLYVINYL CHLORIDE PLASTICS IK RATS (MEETING ABSTRACT) , A TERATOLOGIC (VftlUATl VINYL CHLORIDE POISONING IN THE USSR LITERATURE SURVEY E MONOMER (V C' METABOLIC APPROACH TO INDUSTRIAL POISONING BLOOD KINETICS AND DISTRIBUTION OF **74C VlNYLCHLORIO tlGAl DECISIONS AND OPINIONS IN POLLUTION CASES HEPATIC DISEASE AMONG WORKERS AT A VINYL CHLORIDE POLYMERISATION PLANT PULMONARY CHANGES AMONG VINYL CHlQRtQE POLYMERIZATION WORKERS VINYL CHLORIDE POLYMERS IN CONTACI WITH FOOD ORKERS JN 1HE MANUFACTURE OF VINYL CHLORIDE AND ITS POLYMERS MORTALITY STUDY Of W E ICIRARISlHVDROIYMElHYLfPHOSPHONIUM CHLORIDE AND POLYVINYL BROMIDE MOUSE SKIN CARCINOGENICITY TESTS OF THE FLA IOCHEMICAL AND HlSTOPATHQLOGlCAL CHANGES INDUCED BY POLYVINYL CHLORIDE DUST IN NAT LUNG / SOME B SEG 10 VINYL CHLORIDE MONOMER IH THE MANUFACTURE Of POLYVINYL CHLORIDE IN GREAT BRITAIN MQRIAllTY EXPERIENCE OF W EFFECTS Of POLYVINYL CHLORIOE INGESHON BY DOGS ARDS ASSOCIATED WITH EXPOSURE TO VINYL CHLORIOE AND POLYVINYL CHLORIDE MATERIALS HAZ [RATOLOCIC [VAi.UA I ION OF PLASMA SOLUBLE EXIRAC1S OF POLYVINYL CHLORIDE PLASTICS IN RATS (MEE1ING ABSTRACT) AT ONCOGENIC AND MUIAGENlC RISKS IN COMMUNITIES WITH POLYVINYL DUQRlOf PRODUCTION FACILITIES CROSCOPY CAPILLARY ABNORMALITIES IN POLYVINYL CHLORIDE PRODUCTION WORKERS EXAMIWTION BY IN VIVO Ml FORTHEI RESULTS IN POLYVINYL CHLORIDE PRODUCTION WORKERS ASPECTS LIVER DISEASE IH POLYVINYL CHLORIDE PRODUCTION WORKERS CLINICAL AND LAPAROSCOPIC ECTIQN WITH THE MANUFACTURE OF PLASTIC BOTTLES FROM POLYVINYL CHLORIDE OCCUPATIONAL DISEASES IN CONN ONG CHEMICAL WORKERS INVOLVED IN THE MANUFACTURE OF POLYVINYL CHLORIDE AN EPIDEMIOLOGIC S1UOY QF BlOQG SCREENING AMlC S1UDY PRECURSOR LESIONS IN EXPOSED POPULATIONS AS INDICATORS Of OCCUPATIONAL CANCER RISK P0R1AL HYPERTENSION IN VINYL CHLORIDE MONOMER WORKERS A HEMODYN PORTAL HYPERTENSION TN VINYL CHLORIDE PR00UCH0N WORKERS CARCINOGENESIS INDUCED BY TRACE CONTAMINANTS IH HOWL CANCER RISK POTABLE WATER PRECURSOR LESIONS IN EXPOSED POPULATIONS AS INDICATORS OF OCCUPA PREDICTIVE VALUE OF CARCINOGENESIS BIOASSAYS S THE CARCINOGENIC RISK Of CHEMICALS (PP THE PREDICTIVE VALUE OF TISSUE MEDIATED MUTAGENICITY ASSAYS TO ASSES N BLUE APPLIED SYSTEMATICALLY OURiNG ALL 1HIRDS OF PREGNANCY ON THE FETUSES OF CFV BATS EFFECTS OF VINYL CHLORL LOTIINC IN PVC WORKERS ACUTE HEPATIC INJURY BY VHtYl CHLORIOE IN RATS EFFECTS OF VINYL CHLORIDE EXPOSURES TO RATS ENHANCEMENT OF 1 I OlCHlOfiUCJHYlENF TOXICITY BT HlOftlDE UN DROSOPHILA MELANOGASFER WllH AND WITHOUT INYL CHLORIOE AND VINYL BROMIDE AflER AROCLOR 1264 VINYL CHLORIDE EPIDEMIOLOGICAL STUOIES AND PRFNEOPIASHC AND COLLACENIZING CHANGES IN THE LIVER AND BLOOD C PRE1REAUD WITH PHENOBARBlTAL PRE1REATED WIIH PHEHOBARBITAL PRE1REA1MENT OF FASTED MALE RATS WITH 2 3 EPOXYPROPAN I OL PRE1REATMENT WITH SODIUM PHENOBAR&U URATE MUTAGENIC EFFECTS 0 PREIHEATMENT ACUTE HEPATOTOXIClTY OF ETHYLENE VJNYl ElUORlDE PREVENTIVE MEASURES YINH CBtORlOE MONOMER AND CANCER AGE NIC RISKS IN COMMUNITIES WITH POLYVINYL CHLORIDE VINYL CHLORIOE AND THE I EXPERIENCE OF WORKERS IN A VINYL CHLORIDE MONOMER LIVER DISEASE IN POLYVINYL CHLORIDE CANCER MUR8IOHT IN A GROUP OF SWEDISH VCM AND PVC PRODUCTION AND USE OF VINYL CHLORIDE IMPAC I OF THE LINK BETWEEN PRODUCTION FACILITIES ONCOGENIC AND MU' PRODUCTION OF PVC (MEETING ABS1RACT PRODUCTION PLANT MORTAL! I PRODUCTION WORKERS CLINICAL ANO LAPAROSCOPIC ASPECTS PRODUCTION WORKERS MORIAHTr AND 257 FURTHER RESULTS IN POLYVINYL CHLORIDE PRODUCTION WORKERS 240 CAPILLARY ABNORMALITIES IN POLYVINYL CHLORIDE PRODUCTION WORKERS EXAM iNAI ION BY IN VIVO MICROSCOPY 263 PORTAL HYPERTENSION IN VINYL CHLORIDE PRODUCHON WORKERS 193 IN VARIOUS CHEMICALS (MEETING ABSfftA/ STRUCTURAL PROGNOSTICATION OF CARCINOGENICITY AND TUMOR ENHANCING ACTIVITY 19? IN VARIOUS CHEMICALS (PP 2071 20*4/ STRUCTURAL PROGNOSTICATION Of CARCINOGENICITY AND 1UM0R ENHANCING ACTlVilr 19 ARE OF a*14C VINYL CHLORIDE AND TRANSFORMATION TO PROTEIN ALKYLATING METABOLITES IN VlIRO LIVER MICROSOMAL DPI 66 RCINOGENIC INTERMEDIATES OF ETHYLENE DIHALIDES WITH PROTEIN AND DNA IN MICE ANO RATS TISSUES IN VITRO (MEETING AfiSIR 62 RMLDIATES OF 1HE CARCINOGEN ETHYLENE DlBROMlOE WITH PROTEIN AND DNA IN VlIRO (MEElING ABSTRACT! / IN1ERACU0N Or PO 18 In COVALENT BINDING OF "Ut-VINYL CHLORIDE TO PROTEINS AND NUCLEIC ACIDS IN VlIRO AND IN VIVO (MEETING ABSIRAC 57 CARCINOGEN TRICHLOROETHYLENE TO HEPAllC MICROSOMAL PROTEINS AND TO EXOGENOUS DNA IN VITRO ' COVALENT BINDING OF IM 4? ALKYLATION OF QNA AND PRQUINS LN MICE EXPOSEO TO VINYL CHLORIDE 273 D STATES THE RECULAIlON OF VINYL CHLORIDE (MEET/ PUBLIC HEALTH OECISlOH MAKING ANO THE LEGAL PROCESS IN THE UNJlE 276 PUBLIC HEALTH AUUN05 AT THE HARVARD SCHOOL OF PUBLIC HEALTH VINYL CHLORIDE CAN THE WORKER BE PROTECTED1 276 Yl CHLORIDE CAN THE WORKER BE PROTECTED^ PUBLIC HEALTH ROUNDS AT THE HARVARD SCHOOL OF PUBLIC HEAllH ViN 1*3 INDUCTION Of PULMONARY ADENOMAS JN SI RAIN A MICE BY SUBSF1TU1ED ORGAN OHAuO ES 264 PULMONARY CHANGES AMONG VINYL CHLORIDE POLYMER 12A1 ION WORKERS 159 PULMONARY TUMORS INDUCED IN MICE BY VINYL CHLORIOE MONOMER 296 VINYL CHLORIDE ANO THE PRODUCTION OF PVC {MEETING ABSTRACT) 277 VINYL CHLORIDE PVC AND CANCER 216 MORTALITY AMONG EMPLOYEES OF PVC FABRICATORS 3 MIGRATION QF VINYL CHLORIOE FROM PVC PACKAGINGS 217 AND CANCER MORBIDITY IN A GROUP OF SWEDISH VCM AND PVC PRODUCTION WORKERS MORTAL 11 r 290 THE RISK OF CONSUMERS ANO PVC WORKERS TO VINYL CHLORIDE 265 GENI7LNG CHANGES IN THE LIVER AND BLOOD ClOH INC IN PVC WORKERS PRENCOPLASIIC AND COL1A 24t HIGH RATE OF CHROMOSOMAL ABERRA1IDN IN PVC WORKERS 211 N CASES OF ANGIOSARCOMA Of 1HE LIVER IN SHAWlNlGAN QUEBEC 11 33 ABOUTES OF BENZQlAlPVRENE IN THE ISOLATED PERFUSED RABBIT LUNG PREPARATION (MEETING ABSTRACT) DISTRIBUTION Df ME 66 ISM OF ACTION ANO DISTRIBUTION ANO BINDING OF **14C RADIOACTIVITY FOLLOWING INHALAIlON EXPOSURE >N RAIS I J DICHL 25 NIC SOLVENTS ANO PLASTIC MONOMERS TO REGJS1ER TOTAL RADIOACTIVITY NONVOLAFILE METAEJOilTES AND NON fXlfiAUAfJI f Ml F 707 TVPHIMURIUM STRAINS 1 HOMAN RAT AND MOUSE LIVER MEDIATED MUIACENlClU OF VINYL CHLORIDE iN S 69 METABOLITES QF THE CARCINOGEN TRICHLOROE1HVLENE IN RAT HEPATIC MlCROSOMES COVALiNl INTERACTION Of 45 1HE INTERACTION Of VINYL CHLORIOE WIIH RAF HEPATIC MICROSOMAL CYIOCHROME P 4SQ IN VITRO 94 YIIDEKE CHLORIDE INDUCED UURASTftUCTURAL CHANGES IN RAT LIVER (MEETING ABSTRACT vir< 99 E 10 III TRlCHlOROETHANE AND IRICHLOROETKYlENE ON RAT LIVER AND BRAIN BIOCHEMICAL AND TOKlLOlOGJCAl If I ELIS OF 102 REPAIR OF RAI LIVER DNA IN VIVO DAMAGEC BY ETHYLENE OIBROUlDE 16 TABOLISM OF VINYL CHLORIDE BY THE ISOLATED PERFUSED RAI LIVER PREPARATION OPTAHE AND RAIE 01 ME 90 CHLQRACETALDEHYDE ON DNA SYNTHESIS IN REGENERATING RAI LIVER ' THE EFFECT OF VINYL CHLORIDE MONOMER CHUlRuIlHYUN no YPHIMURIUM II ACTIVATION BY THE ISOLATED PERFUSED RAT LIVER ' THE MUIAGENlC EFFECI OF J 2 COL Hi ORO ETHANE OR SAdUU 93 GICAL CHANGES INDUCED BY POLYVINYL CHLORIOE DUST IN RAT LUNG SOME BlOCHEMiCAi AND HlSIOf A1 HUlU 157 ONCOGENIC RESPONSE OF RAT SKIN LUNGS AND BONES 10 VINYL CHLORIDE 36 INTERACTIONS Of VINYL CHLORIDE WITH RAT LIVER DNA IN VIVO I? DISPOSITION OF 12**14 C VINYL CHLORIDE IN THE RAT 22 PHARMACOKINETICS Of VINYL CHLORIDE IN THE RAT 18826 PHARMACOKINETICS Of VINYLIDENE CHLORIDE IN THE RAT ORAL TOXICITY OF \ I DICHlOROllHYLENl FN THE RAT EFFECTS OF SEX AGE AND fASMNG 251 HIGH RATE OF CHROMOSOMAL ABERRATION IN PvC WORKERS 16 T LIVER PREPARA 1 JON ' UPTAKE ANO RATE Of MllABOlISM OF VINYL CHiOR-Dt BY IHE ISOLATED PEPFDRIO R* 147 SOLUBLE EXERACTS OF POLYVINYL CHLORIDE PLASTICS IN RATS (MEElING ABSTNACTj A UMTUIOGK. EVAIUAIION fir PLASMA 28 1CHIORQEFHYIENI AFTER SINGLE ORAL ADMINISIRATION IN RATS (MEETING ABSTRACT1 UlSPOSlIION AND MEIA601 (U "Ur 305 LORlDl A DOMINANT lElHAL STUDY FN MAlE RAIS AFTER REPEATED EXPOSURES TO VJNiL CHLOPiOt OP ViN.H'JlNt lh 179 INDUCTION Of STOMACH CANCER IN RATS AND MICE BY HALDGENAUD ALlPHAJIlL lUMU.ANI', 150 NHALATION OF ETHYLENE DlBROMlOE DURING GESTATION BY RATS AND MICE ' 89 S1RANSFER4SE AND GLUTATHIONE REDUCTASE IH LIVER OF RATS EXPOSED 10 VINYL CHLORIDE iMEEUM A6SFRACI t.c.irtl C. 54 0 PHARMACOKINETIC PROFILE Of VINYL IDENE CHLORIDE IN RAIS FOLLOWING ORAL ADMINST RAI (ON MF fAbol >' V AN 167 NCE OF HEPAIOCEUULAR TUMORS AND HEMANGIOSARCOMA JN RATS INHALED I 2 OlBROMOEEHAM DNfi[P IHL (ftFlUEN't (! Ot.LiflPAV 971 NFERIM RESUUS OF TWO YEAR TOXICOLOGICAL STUOIES IN RAIS Of VINYUDENE CHLORJPE IMGFifOPAIiU IN IHE [JkiNMNC rtM^1 i, *4 EflEClS Of VINYL CHLORIDE EXPOSURES 10 RAIS PRETREATED WITH PHI NOBARBlUl 85 ACUTE HEPATIC INJURY BT VENTL CHLORIDE IN RATS PRETREATED WITH PHfflObAREDlAi 66 ETHYLENE OlHALlOES WIIH PROTEIN ANO DNA IH MICE ANO RATS TISSUES IN VlIRO )MlEimC ABSIfiAD INXERACKON (U AC' . 189 OROEFHYLENE TOXICIlV BY PRETREATMEH1 OF FASTED MALE RAIS WIIH 2 3 EPOXYPROPAN I Ql ENHANCEVFNl Or J l O'L", 79 SHORT TERM TOXICITY AND REPRODUCTION STUOIES IN RATS WITH HEXACHLOROU ji BtlTADUNE 146 Nir ANO EMBRVOFOXIC EFFECT OF VINYL CHLORIOE IN CFV RAIS STUDY Of THf iEflxrO'if 92 CHLORIDE FOLLOWING SINGLE AND REPEATED EXPOSURE IN RAIS COMPARISON OF IhE f*l( JN- 15 f CHE S CONTAINING METABOLITES OF VINYL CHLORIDE IN RAIS 7* AND REPRODUCTION STUDIES OF HEXACHLOROBUTAOIENE IN RAIS (Ht LHlMlSTP* ACNtUtHOfs*iU.GEINOfJSir'''l' 181 EAR CHRONIC TOUCHY STUDY WIIH HEXACHLOROBUIA01CNE RATS 67 METABOtL&M Of 1/INHlOfNE CHLORIDE BETWEEN MICE ANO RATS 14 THE FATE OF INHALED KNYt CHLORIDE MONOMER iVCMi IN RAIS PF-jOlfS V A * ) r Uif-JPUia '4 PPH-MiVP*1 ' 1l- r l1 20 iHLOftlOE MONOMER ADMINISTERED BY VARIOUS ROU1ES TO RAIS PHAMMlODrNAMiCS ANl IJPIArf <j( . INT 13 C Virm CHLORIOE FOLLOWING INHAlAliON EXPOSURE IN RATS FA(| r 'A VINY1 CHLORIDE AFTER SINGLE ORAL ADMINISTRATION \H RATS FAU O1 iHt BFQlOGJCAl fAlt OF VINYUDENE CHtORlDE IN RATS : j DC AND ITS PCSSlfil E RElAllONSHlP TO ONCOGFNICUY IN RATS DOSE DEPENDENT fA'i Of <t<W Ch.QP bb *3 4l RADlOACHflTY FOLLOWING INHALATION EXPOSURE IN RACS 1 I OlCHLOROEIHYtENE HEPX3010j,:CI(' PROPOSED MESmAN.SV 14E RING ALL IhiROS Of PREGNANCY ON THE FETUSES OF CFV RATS EFFECFS OF VINYL CMlOR'OE ExPOLDPE At ONE ANO IN COVEjNF' 91 1 ANO EFFECTS ON BROMOSULPHAitlN iSSPv CLEARANCE IN RATS VINYL CHlORlDl INDUCED DEPRESSION Of HfPALlC NONPROlEiN 89 cowrENT glutathione s transferase and gcufafhione REDUCTASE IN LIVER OF WATS EXPOSED TO VINYL CHLOfiiUE iMfFMNf, *P 9L ENE OXIDE AND CHLGRACEJALDEHlfOL ON UNA SYNPMtSiS IN REGENtRAIiNG M( LIVER THE tffLC( 0* VINYL LHlDP'DE MJNGtft'r UCC 106402 ill m 287 .'A ;a 79 49 58 9/ 98 II? Ai iL/ m iii 223 m m 139 m ? ii jlb m 266 II 38 IQ 39 2 76 143 IS 107 II? 27? 200 143 130 131 80 303 ?59 ?58 326 188 213 39 S 98 183 m 157 12? 190 ?5 ?79 60 I ?68 230 269 238 1SI 2SI Ml 288 27? 10 170 183 11? 107 200 104 197 193 It FtCIS OF ENVIRONMENTAL CHEMICALS Oft THE GENETIC INC AND THE LEGAL PROCESS IN 1HE UNI1ED STAFES 1HE [ A CASE FOR THE USE 01 LABORATORY BIOASSAY IN THE CHRONIC TOXICITY AftO SHQfil TERR TOXICITY AND G METABOLIC ACriVAIlOft EXAMPLE VINYL CHLORIDE ft BEIWEEN TRICHLOROETHYLENE AND HEPATIC ENDOPLASMIC OETHYlENE INDUCED DEACTIVATION Of LIVER ENOOPUSMIC LIVER ENDOPLASMIC HI ON SACCHAROHVCES CEREVISIAE STRAIN XVT85 L4C FOR VINYllDENC CHLORIDE A IN WAIER. A CANCER COMMUNITY CARCINOGENIC ATED MUIAGENICJTY ASSAYS TO ASSESS 1HE CARCINOGENIC THE EO POPULATIONS AS INDICATORS OF OCCUPATIONAL CANCER MUTAGENIC AND CARCINOGENIC CARCINOGENIC MUTAGENIC ANO TERATOGENIC IS ONCOGENIC AND MLHAGENIC ABSTRACT AllllAT ION Of ONA MO FORMATION OF 1N**4 ETHENOCYJIOINE MOIETIES IN N OF I N**b ETHENO-AOENOSINE j1 ALKYLATION OF IMlOAZOl DERIVATIVES Of NUCLEIC ACID BASES IONA AND OTENHAUY HA2AROOLIS MATERIALS IN THE ATMOSPHERE OF FENCE IN HUMAN TISSUES OF MONOMERS FOR PLASTICS ANO SIRACli/ CHARACTERIZATION Of THE CHEMISTRY ANO RlOGENESIS OF THE U UVEA ME01A1 ED MUTAGENICITY OF VINYL CHLORIDE IN HLORIOE 0I6UTVL PHTHAIA1E ANO LRlCHLOROETHYLENE ON OCCUPATIONAL LORVOS ANO OlHCfl STRUCTURALLY REiAFEO PESTICIDES IN CHARACTERIZATION Of S 9 ACTIVATION OF OBCP IN THE LtfINS WITH 4(4 NITROBEN2YDPYRIOIHC iNBPl OR WITH THE MU1AGENIC EFFECT Of I ? DK.HLQROETHANE ON 1HE MUTAGENIC EFFECT OF I ? OICHlOROETHANE ON SUE MEDIATED MUTAGENICITY Of VINYLIOENE CHLORIDE IN ENE / ARCINOCENIC ANO MUTAGENIC CHEMICALS II HALOGENATEO OtVED IN THE MAN/ AH EPIDEMIOLOGIC STUDY Of BLOOD IAL MUTAGENS; CARCINOGENS / SESSION II CARCINOGEN CITY Of LI DICHL0R0E1HVLCNE IN THE RAT EFFECTS Of TEN CASES Of ANGIOSARCOMA OF THE LIVER IN LORO 11 3) BUTADIENE f MAIOGRAPHIC ANALYSTS OF HALOGENATEO HVORGCARBQHS IN OVER ENDOPLASMIC RfUCUlUM TARGET 0 1HYR0ID FUNCTION AND EVIOENCE FOR I HE SUBCEUUIAR OMOPROPTUPHOSPHA1E TETRAKI${HYOJH>XYMTHV/ HOUSE ONCOGENIC RESPONSE OF RAT ILA MfLANOGASTER WITH ANO WIIH0U1 PRETREA1MENT WITH E METABOLIC INTERACTION BE1WEEN STYRENE ANO ORGANIC ONV01ATIL7 THREE STEP AUTORADIOGRAPHY OF ORGANIC ICROSQMAI lRtCHLOROETHVLENE OXIDATION (MEETING A/ OWLOflANE. 1E1RACHLOROETHYLENE. / VAPOR INFRARED MOCHLOROPROPANf / THE CONDITION OF THE SCINTIGRAPHY Of LIVER ANO HICAL H1S10METRICAL AND ULTRASTRUCTURAL STUOIES OF ORIOE EXPOSURE fftOOCEO LYMPHOCYTE TRANSFORMATION IN SURVEILLANCE OF CRITERIA FOR A RECOMHENOED E OF A BIOLOGICAL EXPOSURE 1EST TO DETERMINE HEALTH OCCUPATIONAL SAFE IT ANO HEALTH SUBSTITUTED VINYLSIIANES / IS j A HIGHLY INDUCTION OF INDUCTION OF PULMONARY ADENOMAS IN f AND TRICHLOROETHYLENE OH SACCHAROMYCES CEREVISIAE ED MUTAGENICITY OF VINYL CHLORIDE IN S TYPHIMURIUM ft SIRUCTURALLV RELATEO PESTICIDES IN SALMONELLA AND DCS CPP 163 171)7 D OLtfJNS VINYL ANO ALLYL ANALOGS AND EPOXIDES)/ G ACTIVIIY IN VARIOUS CHEMICALS {MEETING ABSTRX/ REGULATION OF MICROSOMAL ENZYME SYSTEMS REGULATION OF VINYL CHlGRlDC I MEETING ABSIRACTi PUBLIC HEAL 1N REGULATORY CONIROl PROCEDURE (PP 1797 18051 VINYL CHlOfttO REPAIR OF RAT LIVER ONA IN VIVO 0MlAGED BY ETHYLENE Dl&ROMlDE REPRODUCTION S1U0IES Of HlXACHlQRGBUTADlEHt IN RATS REPRODUCTION STUDIES IN RATS WITH HEAACHLUKM 3 BUTAOICNC RESOLUTION OF DOSE RESPONSE FQIiClTY DATA FOR CHEMICALS REQUIRIN RETICULUM (MEE1ING ASSIRACTt iPP 69 70k HJIERACTIQ RETICULUM ANO GLUTATHIONE DEPLETION i MEETING ABSTRACT] TfitCHL RETICULUM TARGET SITE OF HALOCARBOft METABOLIIES REVERSION STUDIES IMEETING ABSTRACT] GENETIC AND TOMCOlOGlCA REVIEW Of ANIMAL STUDIES (MEETING ABSTRACT) REVIEW Of SELEC1ED LITERATURE ON EIHYLENE 01 BROMIDE (EOB| REVIEW OF THE LITERATURE REVIEW ON THE ifalClTV OF 1RACE AMOUNTS Of TETRACHIXOROErHYLENE RISK AMONG WORKERS EXPOSED TO CHLQROPRENE RISK ASSESSMENT RISK ASSESSMENT EIHYLENE DlGROMlOE RISK Of CHEMICALS (PP 467 4911 / THE PREBlClIVE VALUE OF 1ISS RISK Of CONSUMERS AND PVC WORKERS TO VINYL CHLORIDE RISK , PRECURSOR LESIONS IN EXPOS RISKS ASSOCIATED WITH HALOGENATEO OLEFINS RISKS ASSOCIATED WITH VINYL CHLORIDE RISKS IN COMMUNITIES WITH POLYVINYL CHLORIDE PRODUCTION FAClllli SNA BY MEIUOLITIES OF VINYL CHLORIDE ANO VINYLBROMIDE (MEETING RNA BY VINYL CHLORIDE METABOLITES INVITRO ANO IN VIVO RNA BY VINYL CHLORIDE MEIABOLITES INVITRO ANO IN VIVO FORMATIO RNA) BY METABOLITES Of VINri CHLORIDE IN VIVO ANO IN VllRO {MEtl RUBBER FACTORIES . MEASUREMENT OF SOME P RUBBER MANUFACTURE / EVIOENCE FOR EXIS $9 ACTIVATION Of 08CP tM THE SALMONELLA TEST SYSTEM {MEETING A8 S CONTAINING ME1AB0LITES OF VINYL CHLORIDE IN RATS S TYPHIMURIUM STRAINS HUMAN. RAT ANO MOU SACCHAROMYCES CEREVISIAE STRAIN XV18S 14C FOR REVERSION SlUDlES SAFETY AND HEALTH STANDARDS TOXIC ANO HAZARDOUS SUBSTANCES SALMONELLA ANO SlttPlOMVCES , MUTAGENICITY Of OICH SALMONELLA TEST SYSTEM (MEETING ABSTRACT) SALMONELLA TYPHIMURIUM (MEETING ABSTRACTS . DETECTION OF ELECTR SALMONELLA TYPHIMURIUM I ACTIVATION 1HB00GH CONJUGATION WllH GL SALMONELLA 1VPHIMURIUM It ACTIVATION BY THE ISOLATED PERFUSED SALMONELLA TYPHIMURIUM TA1535 US SATURABLE METABOLISM ANO THE ACUTE TOXICITY OF 1 1 OlCHlOAOETHYL SATURATED HYDROCARBONS / POTENTIAL HALOGENATEO INDUSTRIAL C SCINTIGRAPHY OF LIVER AND SPLEEN IN VINYL CHLORIOE WORKERS SCREENING TESTS ANO ILLNESS HISTORIES AMONG CHEMICAL WORKERS INV SCREENING OBSTACLES AND OPTIONS IN VITRO 1ESTLNG Of ENVIRONMEN Sa AGE ANO TASTING ' ORAL TOIL SHAWINIGAN QUEBEC SHORT TERM TOXICllY AND REPRODUCTION STUDIES IN RATS WITH HEXACH SILICONE HALIDES / GAS CHRO SITE Of HAlGCARBON METABOLITES SUE OF LNHJRV ! 1.1 0ICHL0R0E1HYLENE HEPATOTOXIClTV fFFECl Of SAIN CARCINOGENICITY TESTS Of THE FLAME RETARDANTS 1RIS(?3DIBR SKIN LUNGS ANO BONES TO VINYL CHLORIOE SODIUM PHENOBARBITURATE / MUTAGENIC EFFECTS OF VINYL CHLORIOE 0 SOLVENTS (MEETING ABSTRACT! IPP IQ) / PQSSIBl SOLVENTS AND PLASTIC MONOMERS TO REGISTER TOTAL RADIOACTIVITY N SORPTION Of VINYL CHLORIOE BY SELECTED FOOD CONSTITUENTS SPECTRAL EVIOENCE FOR ? ? 3 TR9CHL0R0 OXIRANE FORMATION DURING M SPEC1R0PHOTOMETRIC IDENTIFICATION Of CHLOROFORM HALOTHANE ME1H SPERM COUNT DEPRESSION IN PESTICIDE APPUCAIORS EXPOSED 10 OIBRO SPLEEN DURING VINYL CHLORIDE DISEASE {MEETING ABSTRACT) SPUEN IN VINYL CHLORIDE WORKERS SPLEENS IN VINYLCHLORIQE DISEASE / ENZYME HISTOCHE SPLENIC CULTURES (MEETING ABSTRACT) / VUM CHL SPONTANEOUS ABORTIONS POWER IN ENVIRONMENTAL MONITORING STANDARD OCCUPATIONAL EXPOSURE TO ALLYL CHLORIDE STANDARDS FOR WORKERS / THE POSSIBLE US STANDARDS TOXIC ANO HAZARDOUS SUBSTANCES S1ERF0SELEC11VE SYNTHESIS Of VINYL BROMIDES AND CHLORIDES VIA 01 STOMACH CANCER IN RATS AND MICE BY HALOGENATEO ALIPHATIC FUMIGAN STRAIN A MICE BY SUBSTITUTED ORGANOHAUOES STRAIN XY185I4C FOR REVERSION STUDIES (MEETING ABSTRACT) / GEN STRAINS / HUMAN RAT ANO MOUSE LIVER MEDIA1 STREPTOMVCES ! MUTAGENICITY Of DlCHlORVOS AND 01HE STRUCTURAL CORRELATIONS OF CARCINOGENIC ANO MUTAGENIC ALKYL HALI STRUCTURAL PARAMETERS ASSOCIATED WITH CARCINOGENESIS (HAlOGEHAFE SIHOCTURAL PROGNOSTICATION Of CARCINOGENICITY ANO TUMOR ENHANCIN 19? :oo 195 194 190 LQ3 104 323 82 187 183 91 289 215 217 175 300 9 90 TO TI6 143 203 148 T34 3?l 131 98 149 146 256 14? 164 L43 288 326 258 329 266 178 297 73 166 4 314 178 72 236 187 30 71 138 139 131 65 76 124 ?72 305 79 78 189 49 188 80 82 185 83 314 16Q 181 70 82 99 14? 33? 171 306 G ACTIVITY IN VARIOUS CHEMICALS (PP 2073. 7084/ MUTAGENICITY Of DlCHlORVOS AND OTHER UTACFNICJTY OF HALOGENATEO OLEFINS A COMPARISON OF CHEMICAL POSSIBLE METABOLIC INTERACTION BETWEEN UIAGTNICITY OF INDUSTRIAL COMPOUND VINYL CHLORIDE FAGENICIIY OF INDUSTRIAL COMPOUNDS VINYL CHLORIDE VINYL CHLORIOE AND VINYL BENZENE I BETA CHLORGPREKEl? CHLOROSllTAOltNE 1 i) ACUIE ANO CT OF ALTERED THYROID FUNCTION AND EVIDENCE FOR THE INDUCUON OF PULMONARY ADENOMAS IN S1RAIN A MICE BY CHLORIDE INDUCED DEPRESSION OF HEPATIC NON PROTEIN VINYL CHLORIDE POISONING IN THE USSR LITERATURE ANGIOSARCOMA OF THE LIVER AN EPIDEMIOLOGIC MORTALITY AND CANCER MORBIDITY IN A GROUP OF CHlORQ E1HERS ANO OF I 4 OICHLOROBUTENE 2 IN ICR/HA R MEMBRANES av CHLORINATED OLEFINS WITH EMPHASIS ON CHL0R0E1HYLENE OXIDE AND CHLORACETALDEHYOE ON ONA (SILANES, A HIGHLY STEREOSELECTIVE CTS Of LIVER EXTRACTS ANO A FREE RADICAL GENERATING ON Of S 9 ACTIVATION OF DBCP IN THE SALMONELLA TEST U TO INDUSTRIAL CHEMICALS MEDICAL SURVEILLANCE ALONE AND IN COMBINATION WITH TRYPAN BLUE APPLIED TRIAL COMPOUNDS EVALUATED BY MEANS OF YEAST GENETIC CALS ON THE GENETIC REGUlAilQN Of MICROSOMAL ENZYME TY OF VINYLIDENE CHLORIDE IN SALMONELLA TYPHIMURIUM LIVER ENDOPLASMIC REltCUtUM ORQBUTADIENE , INHALATION STUDIES TO EVALUATE THE STUOY Of THE CARCINOGENIC. MUTAGENIC AND HIORIDE PLASTICS IN RATS (MEETING ABSTRACT)/ A NIC ACTIVITY OF SOME CHEMICAL SUBSTANCES BY A RAPlO IZAFION Qf S 9 ACTIVATION OF DBCP IN THE SALMONELLA THE POSSIBLE USE OF A BIOLOGICAL EXPOSURE RCINOGEN SCREENING 0SS1ACUS ANO OPTIONS IN VllRO HE MAN/ AN EPIDEMIOLOGIC SJUOt OF BLOOD SCREENING MUTAGENICITY LYMPHOCYTE TRANSFORMATION TETRAKLSlHYDROXYMEFHY/ MOUSE SKIN CAflCINOGENICIlV CURRENT INTELLIGENCE BULLETIN 20 METABOLISM Of TRICHLOROETHYLENE ANO IFICATlON OF CHLOROFORM HALOTHANE METHOXYFLURANE, A REVIEW ON THE TOXICITY OF TRACE AMOUNTS OF FLAME RETARDANTS TRIS(2.3 OlBROMQPROPYDPHOSPHAlE WORKERSDETERMINATION OF AfIC ANGIOSARCOMA IN MAN INDUCED BY VINYL CHLORIDE OICHLOROETHTLENE HEPAIOTOXICHV EFFECl OF AL1ERE0 G ABSTRACT)/ L CHLORIDE EXPOSURE ASSOCIATED LIVER/ URINARY AND Cl I IPP 73)/ ISA OF CHEMICALS {PP / THE PREDIC1IVE VALUE OF A TYPHIMURIUM TA1S35/ ENE DIHALIDES WITH PROTEIN ANO ONA IN MICE ANO RAIS EVIDENCE FOR EXISTENCE IN HUMAN Of HALOGENATEO OLEFINS PRODUCED BY HUMAN AND ANIMAL OCCUPATIONAL SAFETY AND HEALTH STANDARDS BUTADIENE SHORTTERM ,, CHRONIC HI 01/ ENHANCEMENT OF 11 DICHlOfiOETHYLENE PIT VINYL CHLORIDE/ RESOLUTION OF DOSE RESPONSE ANDFASTING/ ORAL SATURABLE Mf TABOLISM AND THE ACUTE U BACUTE TOXICITY/ EFFECT OF VARIOUS TREATMENTS ON THE A REVIEW ON THE INHALATION RESULTS OF A TWO YEAR CHRONIC SM QF CHLORINATED ALKENES AND ALKANES AS RELATEO TO QR0PRENE(2CHL0R0BUTADIENEI 3} ACUTE ANO SUBACUTE ANE AND TRICHL0R0E1HYLENE ON RAT/ BIOCHEMICAL ANO HALATE AND TRICHLOROETHYLENE ON SACC/ GENETIC AND PROSPECTS FDR A REVOLUTION IN THE METHODS OF 0 IN THE DRINKING WA/ INTERIM RESULTS OF FWO YtAft STRUCTURAL PROGNOSTICATION OF CARCINOGENICHV AND TUMP" ENHANUN STRUCTURALLY RELATED PESTICIDES IN SALMONELLA AND STREPTOMVCES STRUCTURE AND ACTIVITY METABOLISM ANO M STRUCTURE REACTIVITY ANO CARCINOGENICITY OF HALOHYDROCARBONS STYRENE AND ORGANIC SOLVENTS (MEETING ABSTRACT) iPP 10) STYRENE AND THEIR POSSIBLE METABOLIIES (MEETING ABSTRACT I (PP STYRENE AND THEIR POSSIBLE MElABOlllES (MEETING ABSTRACT! MU S1YRENEI METABOLISM MUTACENlCIlY ANO CARCINOGENICITY SUBACUTE FOXtCITY TOXICIfr Of SUBCELLLLLAR SITE OT INJURY ] I DICHLOROETHYLENE HEPATOTOXJCJT SUBSTITUTED ORGANOHAUOES SULFHYORYL CONI ENT ANO tf FtCIS ON BROMOSULPHALElN (BSP) CLEARANC SURVEY SURVEY SWEDISH VCM AND PVC PRODUCTION WORKERS SWISS MICE CARCINOGENIC ACTIVITY Of Dl AND TRIFUNCTIONAL Ai SYNERGISM AND ANTAGONISM DAMAGE TO HEPATIC CEltULA SYNTHESIS ANO REACTIONS Of CHIOHOALKENE EPOXIOFS SYNTHESIS IN REGENERATING RAF LIVER THE EFFECT OF VINYL CHLOfl SYNTHESIS Of VINYL BROMIDES ANO CHLORIDES VIA DlSUBSHFUTEO VINY SYSTEM (MEETING ABSTRACT! (PP 1761 VINYL CHLORIDE OEPENOLN SYSTEM (MEETING ABSTRACT) . CHARACTERIZAII SYSTEM FOR NEOPLASTIC AND NON NEOPLASTIC OCCUPATIONAL INJURIES 0 SYSTEMATICALLY DURING ALL THIRDS OF PREGNANCY ON THE FETOSES OF SYSTEMS (MEETING ABSTRACT) MU1AGENICITY OF INDUS SYSTEMS EfftCIS Of ENVIRONMENTAL CHEMI IAIS35 TISSUE MEDIATED MUTAGENICI TARGET SITE OF HALQCARBON METABOLITES TERATOGENIC AND EMBRYOTOXiC POTENTIAL OF BETA CHLOROPRENE <2 CHI TERATOGENIC AND EMBRYOTOXIC EFFECl OF VINYL CHLORIDE IN CFY RAIS TERATOGENIC RISKS ASSOCIATED WITH VINYL CHLORIDE TERATOLOGIC EVALUATION Of PLASMA SOLUBLE EXTRACTS OF POLYVINYL C TEST METHOD DETERMINATION OF THE BLASTOMQGE TEST SYSTEM (MEETING ABSTRACT! / CHARACTER TEST TO DETERMINE HEALTH SIANOAROS FOR WORKERS TESTING Of ENVIRONMENTAL MUTAGENS/CARCINOGENS (PP ?7 33) / SES TESTS AND ILtftfSS HISTORIES AMONG CHEMICAL WORKERS INVOLVED IN 1 TESTS IN CHEMICAL CARCINOGENESIS (PP 229 ?40l TESTS IN VINYL CHLORIOE (VCl WORKERS (MEETING ABSTRACT) TESTS OF THE FLAME RETARDANTS TRIS(2 3 OIBROMOPROPVLtPHOSPHAIE TETRACHLOROE1HYLENE (PERCHlQROETHYLENE) 1E1RACHLOROE1HYLENE IN HUMAN SUBJECTS TETRACHLOROEIHVIENE (PERCHlQROElHVLENF) TETRACHLOROETHYLENE ANO TRICHLOROETHYLENE / VAPOR INFRARED SPE 1E1RACHOLOROETHYLENE IN WATER 1ETRAKIS(HVDROAYMETHYI)PHOSPHONIUM CHLORIDE. AND POLYVINYL BROMI THIODIGLYCOLIC ACID IN URINE SPECIMENS Of VINYL CHLORIDE EXPOSED THOROTRAST AND ARSENIC COMPARISON WITH CASES OF UNKNOWN LliOlO THYROID FUNCTION ANO EVIDENCE FOR THE SUBCELLULAR SITE OF INJURY 11SSUE DISTRIBUTION ANO METABOLISM OF I 2 DIBROMOETHANE {MELON TISSUE GLYCOSAMINOGLYCAN PATTERNS IN ANGIOSARCOMA AND OTHER VINY TISSUE MEDIATED MUTACENlCIlY AND CARCINOGENESIS (MEETING ADSTRA TISSUE MEDIATED MUTAGENlCllT ASSAYS TO ASSESS THE CARCINOGENIC R TISSUE ME0JA1ED MUTACENlCIlY OF VINYLIOENE CHLORIDE IN SALNONLLl TISSUES IN VITRO IMEETING ABS1RAC1) INTERACTION Of ACTIVATED 1 ISSUES Of MONOMERS FOR PLASTICS ANO RUBBER MANUFACTURE TISSUES / ALKYLATING AND MUTAGENIC METABOLITES TOXIC AND HAZARDOUS SUBSTANCES TOXIC EFFECTS OF VINYL CHLORIOE TOXICITY ANO REPRODUCTION STUDIES IN RATS WITH HEXACHLORO (I 3) TOXICllY AND REPRODUCTION STUDIES Of HEAACHlORQBUTAOIEHE IN RATS TOXICITY BY PRETREATHENT OF FASTED MALE RATS WITH 2 3 EPOXYPROPA TOXICllY DATA FOR CHEMICALS REQUIRING METABOLIC ACTIVATION EXAM TOXICITY Of I I OICHlORQLlHYIFNt IN (HE RAT EFFECTS Of SEX AGE TOXICllY Of L.l DICHLOROETHYLENE 10XICITV OF BETA CHLOROPRLNEI2 CHIOROBUIAOIENE 1 3| ACUIE AND S TOXICITY OF INHALED VINYLIOENE CHLORIOE TOXICITY OF SOME HAL0ME1HANES IN MICE TOXICITY OF TRACE AMOUNTS OF TETRACHOLORQfTHYLENE IN WAFER FOXtCITY OF VINYL CHLORIDE AND VINYLIOENE CHLORIDE TOXICITY STUDY WITH HEXACHLOROBUTADUNE FLATS roxicnv / metabuli TOXICllY ' / TOXICITY Of BETA CHL TOXICOLOGICAL EFFECTS OF COMBINED EXPOSURE TO I I l lRlCHLQROElH TOXICOLOGICAL EFFECTS Of ALPHA BENZENE HLXACHLORIDt D19UIYL PHT IOXICOL0GICAL EVALUATION TOXICOLOGICAL SlUOlfS IN RATS 01 VINYLIOENE CHLORIDE FNCORPORAFf TOXICOLOGY QF VINYL CHLORIOE UCC 106403 314 331 m hi m 266 19 154 1% n 154 31 S3 74 22? SI 96 596 142 0 33 57 m 56 93 95 313 4 253 165 35 US 121 1*1 oe 1is93* 167 87 159 184 t 127 107 131 m 94 238 234 207 273 302 It 20 19 1 II 53 72 6 249 24 2*4 2*3 244 88 266 217 1* III 19) 323 41 81 A REVIEW ON (HE TOXICITY Of CARCINOGENESIS INDUCED BY vinyl chloride exposure induced lymphocyte IEME (MEETING ABSTRACT) (PP 78)/ MALIGNANT RN LUNG Of HAMSTER (MEEDMC A/ IN VITRO MALIGNANT IRACTW LYMPHOCYTE YU MICROSOMAL UPTAKE Of *#I*C VINVl CHLORIDE AND RlAl ALIIRHAIIVES l MOLECULAR ASPECTS 10 THE OXIRANE FORMATION OF METABOLISM Of TRANSFORMING ACTIVITIES Of STRAW) |PP 69 70)7 INTERACTION BETWEEN METABOLISM OF MACHINERY PIANT / EVACUATION Of EXPOSURE fO A COHORT STUDY ON MECHANISM FOR YMES 1MEETING ABSTRACT)/ ACUTE VALENT INTERACTION Of METABOLITES Of THE CARCINOGEN GAS LIQUID CHROMATOGRAPHIC DETERMINATION Of S Of COMBINED EXPOSURE 10 I.U IfflCHlOROEIHANE AND F ALPHA BENZENE HEXACKtORIOt DlBUtVL PHUUtME MO 2.2.3 TSICHtOROOXlRANE FORMATION DURING MICROSOMAL 0 / IRREVERSIBLE RINDING Of **UC LABELLED S ONA IN VITR/ COVALENT BINDING Of THE CARCINOGEN CHROMOSOME STUDIES Of OSOME OEPENOENT COVALENF BINDING OF THE CARCINOGEN. CULUM AND GLUTATHIONE OEPltJWN (MEETING ABSTRAC/ 055 OF LIVER GLUTATHIONE IN VIVO/ HALOlHANf. METHOKYfLURANE, UTRACHL0R0ETHYUN1 AND STUDY / HEALTH DAMAGE BV CARCINOGENICITY OF MIC CONCENTRATIONS PRELIMINARY COMMUNICATION / IN ICR/HA SWISS/ CARCINOGENIC ACTIVITY OF 01 AND SHIN CARCINOGENICITY TESTS Of THE NAME RETAROANTS NYL CHLORIDE EXPOSURE ALONE AND Ut COMBINATION WITH STRUCTURAL PROGNOSTICATION OF CARCINOGENICITY AND STRUCTURAL PROGNOSTICATION Of CARCINOGENICITY AND ER THE INFLUENCE Of/ OCCURRENCE Of MEPAIOCEUUUR RESPONSE IN VINVl-CHLORIDE-IHDUtED LIVER INJURY AND PULMONARY IMAMU. AND VINYL CHLORIDE ON fHE INDUCTION Of LIVER 4 (4 WIMWENmiPYRlDlNt OfDP) OR WITH SALMONELLA UTACENIC EFFECT Of 1.2 OlCHLOROETHANE ON SALMONELLA LIVER MEDIATED MUTAGENICITY Of VINYL CHLORIDE IN 5 D MUTAGENICITY OF VINYLIOCNE CHLORIDE IN SALMONELLA UTAGENJC EFFECT Of 1,2 OlCHLOROETHANE ON SALMONELLA VINYLIDENE CHLORIDE INDUCED ENZYME HISIOCHEMKAL. NISTOMEItiCAL AW AllON/ CAL STUDIES Of VINYL CHLORIDE HEALTH EFFECTS IN THE HEALTH OCCJSlON MAKING AND THE LEGAL PROCESS M THE ARCIHOGENIC AND MUTAGENIC CHEMICALS I HALOGENATE0 PERFUSED RAT LIVER PREPARATION / TO RATS / PHARMACODYNAMICS AND IKYLATING METABOLITES IN VITRO/ LIVER MICROSOMAL ETHYLENE OIBAOMIK IN 0 OTHER VmvUHLORlOE EXPOSURE ASSOCIATED LIVER/ DETERMlHATlOK Of TWO VINYL CHLORIDE METABOLITES IN DETERMINATION Of THlODlGLVCOtfC ACID M C DETERMINATION Of TRICHLOROETHYLENE METABOLITES IN VINYL CHLORIDE POISONING W THE S AND DISTRIBUTION Of "*I4C VMYLCHlOfllOt MONOMER ( HALOTHANE. METHOXVflURANE. TETRACHtOtOETHYUME, / DIES ON PERSONS WITH AND WITHOUT VC ILLNCS1 AND ON SOME STUDIES UNDERTAKEN ON PERSONS AND ANIMALS WITH RHAL CHROMOSOME STUDIES ON PERSONS WITH AND WITHOUT INITIAL FEATURES Of VINYL CHLORIDE ( LYMPHOCYTE TRANSFORMATION TESTS IN VINYL CHLORIDE ( ORTAUTV AND CANCER MORBIDITY IN A GROW OF SWEDISH hoo fOR the measurement of vinyl chloride monomer i ICS Of THE FATE Of INHALED VfNVl CHLORIDE MONOMER \ GENETIC EFFECTS INDUCED BY VWYL CHlORlOE MONOMER ( SSOCIATED WITH CARCINOGENESIS (HALOGENATED OLEFINS. 1CITV / VINYL CHlORlOE AMD f ONA AND RNA BY MEIABOLITIES Of VINYL CHLORIDE AND TY Of ETHYLLHt VINYL FLUORIDE YINYt CHLORIDE. AND TRACE AMOUNTS Of TETRACHQLOROEIHYLENE IN WATER TRACE CONTAMINANTS IN POTABLE WATER TRANS-I 4 0ICHLOROBUTENE (PF 1*9 154) TRANSFORMATION IN SPLENIC CULTURES (MEETING ABSTRACT) TRANSFORMATION OF A BABY HAMSTER LUNG CELL LINE OY 2 CHLOROBUTAD TRANSFORMATION OF CELLS Of WHOLE EMBRYOS. FETAL BRAIN AND NEWBO TRANSFORMATION TESTS IN VINYL CHLORIDE |VQ WORKERS (MEETING ABS TRANSFORMATION TO PROTEIN ALKYLATING METABOLITES IN VITRO / LI TRANSFORMING ACTIVITIES Of TRICHLOROETHYLENE Afo PROPOSED INOUST TRICHLOROETHYLENE AND OTHER CHLORINATED ETHYlENES (MEETING ABSTR TRICHLOROETHYLENE AND TETRACHLOROETHYUNE IN HUMAN SUB1ECTS TRICHLOROETHYLENE AND PROPOSED INDUSTRIAL ALTERNATIVES TRICHLOROETHYLENE AND HEPATIC ENDOPLASMIC RETICULUM (MEETING AB TRICHLOROETHYLENE BY THE ISOLATED PERFUSED LUNG TRTGKIOROETHYICNE DURING THE DEGREASING OF SMAU COMPONENTS IN A trichloroethylene exposure and cancer mortality TRICHLOROETHYLENE HEPATOTOXtClTY (MEETING ABSTRACT) TRICHLOROETHYLENE HEPATOTOXKITY AND INDUCTION Of MICROSOMAL EN2 TRICHLOROETHYLENE IN RAT HEPATIC MICR030ME5 / CD TRICHLOROETHYLENE METABOLITES M URINE TRICHLOROETHYLENE ON RAT LIVER AND BRAIN / BIOCHEMICAL AND TOXI IRTCHtOWEFHYlENE ON SACCMttOMYCES CEREVI&IAE STRAW XV1B5 14C f TRtCHLOROCIHYlEHE OXIDATION (MELTING ABSTRACT) / SPECTRAL EVIDE TRICHLOROETHYLENE ID MICE LIVER CONSTITUENTS IN VIVO AND IN VITR TRICHLOROETHYLENE TO HEPATIC MICROSOMAL PROTEINS AND 10 EXOGENOU mCHlOROETHYUNE WORKERS TRICHLOROETHYLENE. TO CCUUUUt MACROMOLECULES (MEETING ABSTRACT TRICHLOROETHYLENE INDUCED DEACTIVATION Of LIVE* ENDOPLASMIC REIi TRICHLOROETHYLENE INDUCED DEACTIVATION OF CYTOCHROME P 450 AND l TRICHLOROETHYLENE- (PP 263 276) fnCHLOROETHYLENE / VAPOR INFRARED SPECTROPHOFOMETRIC 1DENTJFIC TRICHLOROETHYLENE AN EPIDEMIOLOGICAL AND CLINICAL EXPERIMENTAL TRlCHtOftOCIHYlEHC FACT DR ARTIFACT? TRICHLOROETHYLENE: RELATIONSHIP Of METABOLITE LEVELS TO ATMOSPHE TfBFUNCFIONAl ALPHA CHIORO ETHERS AND Of t 4 (XCMOROBUTENE 2 1R1ST2.3 DIBROMOPROPVUPHDSPHATE TETTttKISfHYOfiOXYMETHYUPHDSPHO TRYPAN BLUE APPLIED SYSTEMATICALLY OURWG AU THIRDS OF PREGNAA TUMOR ENHANCING ACTIVITY IN VARIOUS CHEMICALS (MEETING ABSTRACT TUMOR ENHANCING ACTIVITY IN VARIOUS CHEMICALS (PP 2071-2084) TUMORS AND HEMANGK7SMCQMA IN RATS WHALED 1.2 OIBROMOEIKANE UNO TUMORS M MICE (MEETING ABSTRACT) / SOME ASPECTS OH DOSE TUMORS INDUCED IN MICE BY VINYL CHLORIDE MONOMER TUMORS. PRELIMINARY REPORT / EFFECT Of E IYPHIMURIUM (MEETING ABSTRACT) / DETECTION Of ELECTROPHILIC MET TTPHIMURIUM I ACTIVATION THROUGH CONJUGATION WITH GlUTATtttON IN IYPHIMURIUM STRAINS- / HUMAN. RAT AND MOUSE TYPHJMURIUM TAIS3S / TISSUE MEDIATE IYPHIMURIUM W ACTIVATION BV THE ISOLATED PERFUSED RAT LIVER ULTIASIRUCTURAl CHANGES IN RAT LIVER (MEETING ABSTRACT) UtIRASTRUCTURAl STUDIES Of SPLEENS W VWYICHL0R10E DISEASE UtlRASTRUCTURE Of LIVER DAMAGE IN CHRONIC VINYL CHlORJOE INTOXIC UNITEO STATES (MEETING ABSTRACT)- / EPIOEMIOLOGI UNITED STATU THE REGULATION Of VINYL CHLORIDE (MEETING ABSTRAC UNSATURAIEO HYDROCARBONS / POTENTIAL HALQGENATEO INDUSTRIAL C UPTAKE AND RATE OF METABOLISM Of VINYL CHLORIDE BY THE ISOLATED UPTAKE Of VINYL CHLORIDE MONOMER AOMHNSTERED BY VARIOUS ROUTES UPTAKE Of **14C VINYL CHLORIDE AND TRANSFORMATION TO PROFEIN A URBAN AIR URINARY ANQ TISSUE GLYCOSAMINOGLYCAN PATTERNS IN ANGIOSARCOMA AN URINE (MEETING ABSTRACT) URINE SPECIMENS OF VINYL CHLORIDE EXPOSED WORKERS URINE / CAS LIQUID CHROMATOGRAPHI USSR: LITERATURE SURVEY VCM) (MEETING ABSTRACT) / METABOLIC APPROACH TO INDUSTRIAL P VAPOR INFRARED 5PECTROPHOTOME7RIC IDENTIFICATION OF CHLOROFORM. VC EXPOSED ANIMALS / MUTAGENICITY OF VINVL CHLORIDE EXTERNAL C VC JUNES (MEETING ABSIUCT) /. EXTERNAL CHROMO VC ALNESS. AHO ON VC EXPOSED ANIMALS / MUTAGENICITY Of VINYL C VC) HEPATIC INJURY (MEETING ABSTRACT) VC) WORKERS (MEETING ABSTRACT) / VCM AND PYC PRODUCTION WORKERS /M VCM) IN 7UR /A PRACTICAL MET VCM) IN RATS / PRELIMINARY SICO VCM) WIDER MAMMALIAN METABOLIC ACTIVATION STUOIES IN VITRO AND VINYL AND ALLYl ANALOGS AND EPOXIDES) (PP 821) / STRUCTURAL VINYL BEN2ENE (STYRENE) METABOLISM, MUTAGENICITY AND CARCINOGEN VINYL BROMIDE (MEETING ABSTRACT) / ALKYLATION 0 VINYL BROMIDE AFTER AROCIGft 1254 PRETREATMENT / ACUTE HEPAIOIOX 312 IQ 81 312 171 131 26 50 2) 54 67 94 312 185 105 173 161 160 315 172 IQ 65 43 62 39 T8 38 111 40 125 IS5 153 326 47 45 33 19 57 127 18 39 47 33 3? 102 240 36 95 38 111 IQ 132 n 118 135 295 294 171 331 229 314 136 23) 276 2Q4 266 228 212 225 223 246 214 245 23? 202 221 210 258 VINYL HALIDES CARCINOGENICITY A HIGHLY STEREOSELECTIVE SYNTHESIS OF 1254 PRET8EATM/ ACUTE KEPAJOTOXIClTY Of ETHYLENE. NO VINYLIDENE CHLORIDE / ESUUS OF TWO YEAR TOXICOLOGICAL STUDIES IN RATS OF TISSUE MEDIATED MUTAGENICITY Of PHARMACOKINETICS Of PARATIVE MAMMALIAN METABOLISM OF VINYL CHLORIDE AND THE BIOLOGICAL FATE Of METABOLISM AND PHARMACOKINETIC PROfJLE Of DIFFERENCES IN METABOLISM OF (MEETING ABSTRACT) / CARCINOGENICITY VINYL BROMIDE. VINYL CHLORIDE. AND EFFECT OF VARIOUS TREATMENTS ON TOXICITY Of INHALED RATS AFTER REPEATED EXPOSURES TO VINYL CHLORIDE OR CAAC1NQGCNICITV STUDIES ON CARCINOGENICITY Of VINYL CHLORIDE AND INHALATION TOXICITY OF VINYL CHLORIDE AND CARCINOGENICITY SlOASSAYS OF S OF VINYL BROMIDES AND CHLORIDES VU DISUB5TH0TED ES WITH PROTEIN AND ONA IN MICE AND RATS TISSUES M THE METABOLIC ACTIVATION Of VINYL CHLORIDE IK RC1NQGCN ETHYLENE OISROMlDE WITH PROTEIN AW) PNA IN HA) BY METABOLITES Of VINYL CHLORIDE IN VIVO AND IN 14C VINYL CHLORIDE TO PROTEINS AND NUCLEIC ACIDS IN NE MOIETIES IN RNA BY VINrt CHLORIDE METABOLITES IN M) UNDER MAMMALIAN METABOLIC ACTIVATION STUDIES IN ALKYLATION Of RNA BY VINYL CHLORIDE METABOLITES IN HRONOSOMAL ABERRATIONS AS AH ANALYTICAL TOOL FOR IN NO TREATED WITH CHEMICAL CARCINOGENS. MAINTAINED W BRAIN. AND NEWBORN LUNG OF HAMSTER (MEETING A/ IN II CARCINOGEN SCREENING OBSTACLES AND OPTIONS IN AND 36CILA8ELE0 VINVL CHLORIDE IN VIVO AW) IN IDE WITH RAT HEPATIC MICROSOMAL CYTOCHROME P 450 IN OETHYLENE TO MICE LIVER CONSTITUENTS IN VIVO AND IN TRANSFORMATION TO PROTEIN ALKYLATING METABOLITES JN HEPATIC MICROSOMAL PROTEINS AND TO EXOGENOUS OHA IN ACTIVATION THROUGH CONJUGATION WUH GLUTATHION IN ORIDE TO PROTEINS AND NUCLEIC ACI05 JN VITRO AND IN S {ONA AND RNA) BY METABOLITES Of VINVL CHlOJHOE IN USM OF 14C AND 3SCI LABELED VINYL CHLORIDE JN LEO TRICHLOROETHYLENE TO MICE LIVER CONSTITUENTS IN TUW Of ETHENO DERIVATIVES OF NUCLEIC ACID BASES IN REPAIR OF RAT LIVER ONA IN INYL CHLORIDE PRODUCTION WOfKCRS EXAMINATION BY IN HTEftAClIQNS Of VINYL CHLORIDE WITH RAT-LIVER ONA IN f CYTOCHROME P 450 AND LOSS Of LIVER GLUTATHIONE IN N RNA BY VINYL CHLORIDE METABOLITES IN VITRO AND IN AllAN METABOLIC ACTIVATION STUOIES IN VITRO AND IN f RNA BY VINVl CHLORIDE METABOLITES IN VITRO AND IN MUTAGENICITY OF 1943 1949)/ ACT) / THE MUTAGENICITY Of AGENIC ACTIVITY OF CHEMICALS IDENTIFIED IN DRINKING ASSOCIATION Of DIOREFRACTORIES IN DRINKING ENCES / DRINKING OF VINYLIDENE CHLORIDE INCORPORATED IN 7HE DRINKING INOGENESIS INDUCED BY TRACE CONTAMINANTS IN POTABLE CARCINOGENS IN DRINKING OKICITY OF TRACE AMOUNTS OF (EIRACHOLUROCTHYLENE IN HALOGENATEO COMPOUNDS FOUND W CHLORINATED DRINKING S OF CANCER MORTALITY WITH HALOMCTHAHES IN DRINKING RD SCHOOL Of PUBLIC HEALTH VINYL CHLORIDE CAN THE PRIMARY LIVER CANCER DETECTION IN VINYL CHLORIDE PHOCYTE TRANSFORMATION TESTS IN VINYL CHLORIDE (VC) MORTALITY AMONG UUNORY AND DRY CLEANING HEPATIC DISEASE AMONG MORTALITY Of CANCER RISK AMONG CYTOGENETIC INVESTIGATIONS ON LYMPHOCYTES FROM POLYVINYL CHLORIDE IN GR/ MORTALITY EXPERIENCE OF CYTOGENETIC INVESTIGATIONS ON LYMPHOCYTES FROM CAL DESCRIPTION Of THE 1/ HEPATIC ANGIOSARCOMA IN MORTALITY EXPERIENCE Of A5ES Of ANGIOSARCOMA OF THE LIVER IN VINYL CHlORlOE MORTALITY STUDY OF CREENING TESTS ANO (UNCSS HISTORIES AMONG CHEMICAL UCC 106404 '90 THE RISK Of CONSUMERS AMO PVC WORKERS TO VINYL CHLORIDE *M ilyIR DISEASE iff PQlTVWm CHLORIDE PRODUCTION WORKERS CLINICAL AND LAPAROSCOPIC ASPECTS /? c acid in urine specimens of vinyl chloride exposed WORKERS 2ht rtsi tocm exposure determine health standards for WORKERS DETERMINATION Of IHIOOlGLYCOtl THE POSSIBLE USE Of A BIOlOGl SI! PROPORTIONAL MORTALITY AMONG Vni CHMMlOi WORKERS '.Vi FURTHER lf(SOUS <N POLYVINIft CHlORlDi PRODUCTION WORKERS ?l5 rCATUAfS (Jf HEPATIC ANGIOSARCOMA IN wun CHiQRIDE WORKERS I'65 Z'NC CHANCES J* THE LIVER AND BLOOD CLOTTING IN Pvt WORKERS CLINICAL AND MORPHOLOGIC PHENEOPlASUC ANO CQllAGENt BioiTr jn a croup of Swedish vcm and pvc production WORKERS MORTALITY AND CANCER MOB ?6j PORTAL HYPERTENSION IN VINYL CHLORIDE PRODUCTION WORKERS 2W CE Of A COHORT Of VINYL. CHLORIDE POLYVINYL CHlORlOE WORKERS MORTALITY EXPCftlCft m CHROMOSOME SlUOllS Of IRiCHCORQETHTUNE WORKERS ?% A OF THE LIVER IN VINYL CHLORIOE POLYVINYL CHlORlOE WORKERS ANCIOSARCOM JSI HIGH RATE Of CHROMOSOMAL ABERRATION JN PVC WORKERS ??4 LONG CANCER INCIDENCE AMONG CHlOROPRfNE HANDLING WORKERS 26* LMONARY CHANGES AMONG VINYL CHLORIDE POLYMERIZATION WORKERS PU -AT LHKQMOSOMAL ANALYSES 1H Vm GHUWiDE EXPOSED WORKERS .'59 SCINTIGRAPHY Of LIVER AND SPLEEN IN VINYL CHLORIDE WORKERS :fi.} PORTAL HYPERTENSION IN VINYL CHLORIDE HONONER WORKERS A HEMODYNAMIC STUDY jju iary abnormalities jn polyvinyl chloride production WORKERS EXAMINATION BY IN Vl*0 MICROSCOPY 2~A A Of IrtE LIVER IN VINYL CHLORIDE POLYVINYL CHlORlOE WORKERS 19/? UPDA1L OF THE NtOSH REGISUft CAPII ANCIOSARCOM I'Si ENVIRONMENTAL CONCERNS BEYOND THE WORKPLACE 14? RICHLOROETHYLENE ON SACCHARQMYCES CEREtflSiKE STRAIN XV]85 HC FOR REVERSION STUDIES iMEFTINC ABSTRACT GENETIC AH Hi (CITY Of INDUSTRIAL COMPOUNDS EVALUATED BY MEANS Of YEAST GENETIC SYSTEMS i MEETING ABSTRACT i MUTAGEN ItS D GENE CONVERSIONS BY VINYL CHLORIDE METABOLITES IN YEAST INDUCTION Of GENE MUTATIONS AN AUTHOR INDEX WITH CITATION NUMBERS Abbondandolo A 103. 104 Adams WCj . 247 Agarwal DK ... ..............93 Alexander V .... . ...312 Allcmand H ........ .............61 Ames BN........ , . .113, 326 Andersen 80. 94, 188. 189 Anderson D .. .106, 247 Anderson H .. ..........264 Andersson K....... ........ 227 Andlauer P....... .........284 Andrews AW ... , ........ 120 Antweiler H......... ......... 51 Appel K.E............. ............18 Archimbaud M .. .........284 Aronson 1............. ....43. 45 Arvanpur J.......... .........269 Author not iden.. ....... 174 Axelson O............. .........227 Buchner U .......... .........265 Baden J ................ .........132 Bahlman LJ ........ .166. 312 Baier EJ................ .........166 Buiiev K................. ...95. 97 Ballard JJ............. ..78. 181 Balmer MF.......... .........171 Banerjee S56, 57, 58, 59, 62, 63. 65 Barale RI03, 104, 110, III. 115 Barbm A .,68. 114, 124 Barnes AW.......... .........296 Baroncelli S103, 104. no. III, 115 Bartsch H68, 107, 108, 114, 115. 124. 138. 139, 182. 329 Basuk J .............. .........309 Bauer C............. . .........Ill Bechtelsheimer. ........... 232 Beesau O......152, 153. 155 Beije B............................ 130 Benda P ........................ 453 Benhamou JP.................. 61 Bergman K ..................... 25 Berk PD................. ........ 304 Bertrand R ........... ........ 284 Bhandari JC. 160. 161. 167 Biersack HJ............ ......259 Bieotti A .............. ....... 157 Biles R ................... 143, 300 Biles RW................ ....... J44 Bimboes D............. ....... 125 Bingham E............. ....31.55 184, 312 Biran D................. ....... 279 Btshop M .............. ....... 215 Blair A.................... ....228 Blendis LM............ ....... 262 Blum B................... ....... 294 Boden LI................ ....... 276 Bognur Z ................ ....... 251 Boll HMI2. 18, 19, 21. 22, 32, 37, 38. 39, 40, 52 Bolt W ............. 12,19.261 Bonaili S.............. 103. 104 Bonse O .60. 140, 196, 198 Boor PJ.................... 95. 97 Border EA...................... 90 Borzelteca JF ................... 83 Bowman FJ........................83 Bradv J ........................ 715 Braun WH...................... 64 Brem H.................. 187 Bresil H............................. * *** Bnnkenhoff M.............. '32 BronzetL GI03, 104, 1)0. Ill, 115 Brown DP.........-........ 320 Brown PG ................... 184 Brunnemunn KD........... I * Buchanan JW ................258 Buchter A-.12, 19. 22. 261 Buffler PA...................202 Burgess WA.................. 27b Burnett W..................... 215 Burrows L........................*26 Bykhovskii AV ........... 220 Bvren D...................... 217 Calleman CJ................... 42 Cummellini A103. '04, 110. III. 115 Campbell DR............. 258 Cantor K.P...................231 Caputo A ............. *57, 173 Carcinogenesis ............. *70 Cardamone G..............200 Carere A..................... 200 Carter SA.................... 280 Cercignant G............... * * * Chalabreysse 3.............284 Chamaillard L............. *55 Chen TS.......................>47 Chtazze L....................... 216 Chieco P.......................*22 Christopherson.............235 Clanton J......................235 Clary )J................... 82, 149 Coffman L................... Coffman LJ..................*87 Collier PF.....................214 Connor T..... ........*43. 300 Connor TH.................. *44 Conolly RB........... " Corbett TH.....................319 Corsi C .103, 104, 111. U5 Corsi G........................ * * Cortese R.....................*45 Con. G.................. *03. *04 Coin G.........................*^ Creech J.............. 204. 304Creech JL....203. 212, 235 Croisy A........................ Crossley 1R..................263 Culik R............................. 149 Czeizel A..........................251 Dalbey W ................. 31. 55 Darke CS ....................... 267 Davies JN.........................215 De Engelse L...................218 De Lorenzo F..................145 De Serres FJ ................ 332 De Verdier A ................227 Decat G ................ 245, 246 Decoune P....................... 228 Decuyper LJ.................... 245 Degl'lnnoeenti .......... 145 Degott C ......................... 61 Delmore F....................... 235 Delorme F ...213, 221, 237 Department of L............272 Descatotre V ................... 61 Dever ME.........................266 Dittenber DA .........78, 181 Diubankova EN.............220 Dixon RL ..............160. 161 Doll AH........................... 258 Downs TD ......................271 Dowty BJ ....................... 295 Drevon C ......................... 156 Drew RT............................84 Du JT ................................ 89 Dumont CR.................... 248 Duport J ..........................284 Duprat P............................24 Eckert WR........................... 3 Eder E..................... 165. 191 Egert G .......................... 125 Egli H................................265 Ehrenberg L...................... 42 Eider C.............................202 Elmore JD........................112 Emmelot P....................... 218 Engholm G .................... 217 Englund A.............. 217, 330 Espinosa E....................... 266 Etzel F.............................. 265 Evans WD....................... 262 Fabry JP............................24 Fadell EH.........................235 Falk H ,.207, 212. 236, 304 Feldmann G...................... 61 Felton JS..........................321 Fernandes J...................... 147 Feron VJ............................82 Fichidzhian BS...............163 Filser JG.....................21, 32 Fishbein LI97. 302. 303, 322 Flake RE.......................... 249 Fletg 1....................... 243, 244 Folly G................ ...146. 148 Fortwengler HP ,..,...,.266 Fox AJ.............................. 214 Frank AL.........................334 Fredrickson DS ..............169 French JE................... 80, 94 Frezza D......103, 104, 115 F'umero S.............. 134 GaiTev WR........... Gamble J................ 210 248 Gargas ML.......... ...29 . 80 Garibian DKh .... 163, 164 Garro AJ................ 116 Gaitelet M............. 284 Gay PC................... 249 Gedigk P......232, 234. 260 Gehring PJI3, .14, 15, 17, 23 . 26. 49, 54. 91. 92, 101, 151. 299. 327 Gervasi G.......................11 I Glass R1........................... 268 Goggelmann W...............125 Goldschmidt BM56. 175, 177 Goldstein RC................. 177 Gothe R............................42,123 Gradiski D.........................24 Grauman D....................228 Green T......................35, 36 Greenberg RA................203 Greenwald P...................215 Greim H.......................... 125,198 G ross R........................... 261 Guengertch FP.,.44. 47. 48 Gupta BN...........................84 Gutlenplan JB................ 116 Haberlandt W................. 252 Habermann RT...............179 Haley TJ ................311. 315 Hammond AR. ..................I Hammond EC................209 Hansteen IL.................. .250 Harleman JH.................... 79 Harper C............................84 Harper P..........................215 Hassett CM......................154 Hathway DE27, 35, 36, 50, 67. 131 Heath CW.......................248 Heath W..........................212 Hecht SS............................ II Hefner RE................. 14, 91 Heilbronner H................252 Heldaas SS....................... 250 Henschler DI6, 60. 70, 133. 140. 165, 195, 196, 198 Herweg K ........................... 72 Heuse A........................... 306 Heusermann U ....238, 239 Hill DL...............................64 Hillestad L....................... 250 Hills JP............................ 298 Hirayama T.................... 190 Hilt B................................*32 Hochstrasser DL........... 258 Hodge MC....................... 106 Hoffmann D..................... II Hogstedt C..................... 227 Holder BB.......................208 83 UCC 106405 Holmherg B........ ...86, 87, 227. 330 Hong CB.......................... 167 Hoover R .......................231 Hornyak E.......................251 House WB............... 160. 161 Hubert-Haburt M.152, 155 Hublel P..........................307 Huduk A........... 146. 148 Hultmark D...................... 42 Humiston CG 78, 171. 181 Hussain S .. 109 I ARC Working Grl76. 313. 316 Ikeda M ................ 73, 190 Infante PF226, 255, 256, 287, 312, 320, 325 Kanetich KM .......... 43. 45 Jacobson CB.................. 242 Jaeger RJ66, 81, 85. 187, ' 276 Jenkins LJ29, 80, 94, 188, 189 Jersey GC.........................78,181 Johnson MN2I I. 212, 235, 240 Johnson WS...................... 77 Johnston TP...................... 64 Jones BK . .27, 67, 131 Jones RA.......... 29, 80, 189 Juhe S............................. 257 Kahn E............................. 268 Kalas D..............................74 Kaminski R .................... 201 Kappus H.... 12, 18. 19, 22 Katz ID ...................... 43, 45 Kaufmann R..................... 18 Kauhanen K ................... 135 Kaw JL...............................93 Kelly DP.......................... 149 Kev MM..........................212 Keyes DG.........................78,181 Khachatryan EA........... 224 Kilian DJ ....202. 242. 249 Kline J.............................. 254 Kline SA................. 8. 9. 62 Kobayashi Y...................219 Kociba RJ................78, 181 Kometzko H ......... 252. 253 Kotchen JM.................... 258 Kover FD........................ 317 Kramer M ........................125 Kraybill HF.................... 331 Kronevi T......................... 86,87 Kupchella CE................... 71 Kuroki T...........................156 Kusters E...........................72 Laib RJ22, 37, 38.39, 40,41 Lane JM........................... 312 Lange CE257, 259. 260. 265. 285, 305, 308 Langner RR.................... 208 Larson E...........................184 Laseter JL.........................295 Lassiter DV......................274 Laumbach AD.112, 117 Lawrie BW ......................262 LeRoy EC....................... 240 Lee CCI05. 150, 160, 161. 167, |85 Lefevre MJ ......................245 Legator M ..............143. 300 Legator MS.................. 144 Leihman KC ................... 69 Leidel NA......................... 166 Leinster P.............................7 Lelbach W K......... 232. 260 Leonard A...............245, 246 Leonard ED............245, 246 Leporini CI03, 104, NO, III. 115 Levitt RC ......................321 Levy S.................... 153, 155 Lewandowski M............. 147 Liberatore F.................... 215 Libs R ........................... 264 Lloyd JW................ 206, 223 Loewengart G............ 178 Loprieno NI03, 104, 110. III. 115 Lonncz M ..............146, 148 Lu PY..................................34 Lyness RN....................... 268 Madrid EO .............. 13. 54 Magadur JL...................... 24 Magnusson J........... 121, 122 Makk L......... 203, 235. 237 Malaveille C68. 107. 114. 124. 139 Mailoni Cl58. 172. 241. 310. 318, 324. 333 Mandel D...........................34 Mansfield Jl............154 Mancq HR ............... 240 Markovits P 152, 153, 155 Marsteller HJ232, 234, 260 Marlin JF................304 Mason TJ................ 231 Matufujt H ..................... 289 Mazzaccaro A ....103, 104 Mazze R................... 132 McCabe LJ............. 231 McCann J................ 113 McCoy EC...............126 McGarvey G..................... 10 McGowan GR........... 13, 15 McKenna MJ ...........26, 54 Melchionne S ..............178 Mengle DC.... ......... 268 Metcalf RL........................34 Metzler M ........................165 Miller A........................... 264 Miller RB........................... 10 Miller RW ......................229 Milvv P .........................116 Minor JL......105, 150, 185 Molina G ........................ 227 Mondino A...................... 134 Monson RR.................... 211 Montesano R.......... 107, 108, 114,124, 139, 156, 182 Morisi L............................172 Morpurgo G ...................200 Mortclmans K................ 135 Moslcn MT85, 95, 96, 97, 100 Muller G...........................53,72 Muller KT.......................261 Muller N....................... 265 Muller R................. 232. 260 Munson AE .....................83 Murdoch IA......................... I Murphy SD........................85 NIOSH .............................301 Nachtomi E.....................102 Nashed N......................... 186 National Cancer. .168, 180 National Instil................297 Nebert DW......................321 Neudecker T........... 165. 191 Nicaise C.......................... 245 Nichols A.........................309 Nichols WE.................... 216 Nicholson WJ................. 209 Nieri R103, 104. 110, II I, 115 Nisbet 1C..........................291 Schlatter CH ................290 Schmeltz I.......................... ii Schmidt AM ................278 Nomiyuma H ..................... 6 Schmidt D........................... 5 Nomisama K ............... .6 Schmidt RE .....................77 Norpoth K.................53, 72 Schumann AM ...............101 Nutt A ................................. 2 Schwartzman G.................4 Nvlander PO................ 128 Schweitzer GE........... 283 Ogden LL........................ 235 Schweiz BA....78. 171, 181 Olofsson H ................. 128 Segerback D................... 42 Olson WA........................179 Seidman H..................... 209 Orlando MM ............ 321 Seidman I................ 175. 178 Ortali VA...................... 200 Seiftcr J.................... 150. 185 Ortiz E................................ 69 Semen W............................79 Osterman-Golkar. ,42. 109 Selikoff IJ88, 209. 236, 264. Oil MG ..................208, 299 304 Oltenwalder H.......... 37. 41 Seth PK ............................ 93 Owen R...,....................... 222 Shahin MM .......... 119. 142 Paddle GM......................247 Sharma RP......................151 Papadopoulo D.. .152, 155 Shih TW.................... 30. 64 Papoian SA...................... 164 Shimkm MB................ 183 Park CN......................... ..49,299 Shoner LG............. .66 Parker JC ......................166 Short RD ... 105, 150. 185 Pegg DG.............................17 Silengo L...................... 145 Pell S................................. 225 Simmon V .............113, 132 Perry R................................. 7 Simmon VF 135, 136. 199 Pessasre D......................... 61 Slaughter JC....................258 Peters JM....................... 211,276 Smith AC................ 177, 178 Peters PJ ....................... 160 Smith GF.......................... 75 Plaffli P.......................... 99 Smith PM...................... 263 Picciano DJ............ 242. 249 Smithe PM ....................262 Planche G ................68, 124 Solomon JJ..........................9 Plummer N........................34 Songster CL.................... 235 Pogosova AM..................163 Spinas R....................... 201 Poirer LA ......................183 Srivastava SP.................... 93 Polan A............................. 215 Stafford J ...................... 286 Poplawski S ......................60 Stein AB............................137 Poplawski-Tabar..............33 Stein G.........257, 305. 308 Popper H.........88, 236, 304 Stein HP................... 166 Potter HR........................ 205 Stein Z........................... 254 Powell KE ......................268 Stemmer KL............... 184 Pregaglia GF ................. 275 Stephens MR...................262 Price PJ............................. 154 Streips UN ............112, 117 Purchase IF.............106, 247 Streitwieser D................. 113 Quasi JF 78, 101, 171, 181 Stretli G............................Ill Radike MJ....................... 184 Strickland TW......... 44, 48 Radwan Z........................... 16 Strobmo B...................... 254 Ramel Cl 18. 121. 122, 127, Struck RF.......................... 64 129 Stutte HJ................. 238. 239 Rampy LW .....................171 Suarez L........................... 202 Ramsey JC.......................299 Sundvall A....................... 127 Rannug UII8, 123, 127, Susser M ..........................254 129, 130 Suzuki Y.......................... 159 Rasmuson B.....................128 Svahlin H .........................128 Rath HJ................................5 Szabo S.............,81, 85. 95, Reichert D......................... 28 96, 97, 187 Reill G.............................. 252 Szentesi I...........................251 Reitz RH.......................... 101 Tabershaw IR................. 210 Reuzel PG ........................82 Talley FA.......................... 84 Reynolds ES85, 95, 96. 97, Tamburro CH7I, 89, 203. 98, 100 204. 230. 266 Richardson CR.......... .247 Tardiff R.......................... 135 Rosellim D . 103. 104, 115 Tardiff RG ................... 136 Rosenkranz HS126, 137, Tatrai E................... 146, 148 141 Teisinger J....................... 288 Rossi A..............................104 Telles NC........................236 Rossi AM 103, NO, III. 115 Teulon F...................... 284 Rowe VK .........................282 Thetss JC...................... 183 Rudolph FB........................ 3 Thelen M........................ 259 Ruocco A......................... 145 Theriault G................... 213 Salmon AG................. 46 Thiess AM............... 243. 244 San Luis T....................... 259 Thiis-Evensen E.............250 Sarma DS ....................... 102 Thomas AW.................... 166 Savolainen H.....................99 Thomas L........................ 304 Schaflner F........................88 Thomas LB..................... 236 Schattenberg PJ .......... 234 Timar N......................... 251 Schlatter C .................... 314 Tjalve H............................. 25 84 UCC 106406 Tomans L........................114 rmovie V .................... 234 Tricir N....................... M2 TritrlI NM........................144 Turchi G ........................in Uehiki H............................ ft Udnoon S .................... 267 Uehlcke II..................... 33. 60 Ungvarv G............ 148. 251 Ungvar; GY.................. 146 Ut/mger R......................314 Vaiino II................... 99. 123 V aleitluie (. R .................... 120 Van DLUKLN 81. 175 Van IJuuren BI.8, 9, 56. 57, 58. 54, 62. 63. 65. 177. 178. |42, 193, 194 Van l.sch GJ ..............281 Van l.ogten MJ ........ 281 Veltman G257, 259. 285, 305, 308 Versm E............................ 72 Vianna N.......................... 215 Viola PL. . .157, 173 VomBruek CG................... 3 Von Borstel RC . ., 142 Wachmester CA . ..42 Waehtmeister CA 123 Wade CL ...78.181 Wagoner JK226. 256, 287. 312. 320 Walker AL......................267 W arhurlon 13 ,,. .. 254 Ward AM .................... 267 Ward JM .................. 179 Waianahe P .................. 304 Walanahe PG13. 14. 15, 17, 23. 26. 47. 49. 91, O'* 101, 327 Watkins J ......................267 Waxmeiler RJ................ 207 Waxweiler RJ.......248, 256 Webster L......................... 90 Wegman DH..................276 Wetchardt H ...............252 Weinbren K................... 233 Weisburger EK ,,162. 179 W'eisburger JH ....... 179 Werner HW ....................28 Westerhulm P............ 217 Wharton R ....................132 Whelan JG ...........203. 204 W'helslone CL ............. 240 Wdliams DM ................ 263 Williamson K.S............ 328 Wimmer J........................... 5 Wmell M ..................86, 87 Winkler C....................... 259 Winston JM 105, 150, 160. 161, 167, 185 Withev JR .....................20 Wolf BS.......................... 166 Wolff MS ...................... 76 Wong JL................. 112. I 17 Wong LC....................... 167 Wong O .......................216 Wood S .................... 202 Woods JS ......... 160. 161 Wyatt RH......................258 Wyatt S........................... 270 Wynder EL................... II Young JD .................... 327 Young RJ................... 7. 226 Young RS....................... 304 Zaunstowski ES............ 120 Zempel JA......... 17, 54. 92 ZilTian VNI63 85 UCC 106407 11.8. DEPARTMENT F COMMERCE National Tachnlcai Information 8arvlca 5205 Port Aoyot flood Bprmgfloid, Vo. 22161 OFFICIAL BUSINESS Ponotty for Privoto Uoo. 6300 PRINTED MATTER POSTAGE AND FEES PAID U.S. DEPARTMENT OF COMMERCE COM-811 THIRD CLASS BULK RATE UCC 106408