Document e15yVd92px7Nmp3OmEJ8a91Gp

AR 226- 050M ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as. PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro- , ammonium salt, CAS # 3825-26-1) Remarks: The 3M production lot number was 37. The test sample is FC143. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 96.5 - 100% test substance and 0 -- 3.5% Cs, C7, andCsperfluoro analogue compounds. METHOD: Method: USEPA 660/3-75-009, 1975 Type: Static acute GLP: No Year completed: 1980 Species: Pimephales promelas Supplier: Dale Fattig of Brady, Nebraska. Analytical monitoring: Temperature, pH and DO Exposure period: 96-hours Statistical methods: Probit analysis Test fish age: Not noted. Length and weight: Average length = 3.6 cm Average weight = 0.40 g Pretreatment: Not noted. Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry: Temperature: 17C. Dissolved Oxygen: 10.0 ppm pH: 8.0 Test conditions remarks: A daily photoperiod of 16 hours light and 8 hours dard, with a 30 minute transition period was maintained throughout the acclimation and testing period. Stock and test solution preparation: Not noted Concentrations dosing rate: Once Stability of the test chemical solutions: Notnoted Exposure vessels: 35 X 20 X 25 cm deep glass aquariums containing 16 liters test solution. Number of replicates: two Number of fish per replicate: 20 Loading rate: 0.50 g/L Numberof concentrations: five plus a blank control Water chemistry during the study: C03780 Dissolved oxygen range (24 -- 96 hours): 4.0-5.0 mg/L pH range (24- 96 Test temperature hours): (24 -- 96 h7o.ur9s8).:018-19C RESULTS Nominal Element vcaolnucee:nt9r6a-thioounrs:LCB5k0c=ont7r6o6l,(576403,-67508,9)75m0g,/L870, and 1000 mg/L Element value based on nominal concentrations. CONCLUSIONS Tmhg/eLtewsitthsaam9pl5e%9C6o-nhfoiudreLnCcseoIfnotrerfvaatlohefad74m3inton7o8w9wmags/Ld.etermined to be 766 `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: the time it wKaisimciosncdhurcatnekdi.ngH2o.weTveesrt,insgammepeltesputrhietycrwiatesrianoftorpqruoapleirtlyytesting at characterized and it lacks analytical confirmation of test substance concentrations. REFERENCES 3LRM.Te5c6h2n5icSa,lRReeppoorrttNSuummbmearry0,37A,qPuraotjiecctToNxiucmibtyerTe8st8i7n0g0:12F6C0-01,43M.(LTo.t-37) Elnabarawy, May 8, 1980. OTHER Last changed: 5/24/00 03781 am: TECHNICAL REPORT SUMMARY TO: TECHNICAL COMMUNICATIONS CENTER -- 201.26 nioran pots iteonbo stsfsa,mdtcw opi0TeCCs) * 5"" wr Environmental Laboratory (EE & BC), St. Paul, Minnesota Commercial Chemicals - ae0535 9970012600 = Aquatic Toxicity Testing: /=F=C-=143) (Lot-37) L.R. 5625S E. A. Reiner 037 = =M. T. Elnabarawy - 2-3% 46P98a1sa - 50012: 23 : 5 secunrvp 8Oo BiRunormion | MhESEIE vaEe evwonronss,fro9mM CURRENT GRECTIVE: Sawe To determine the acute aquatic'LC.) following exposure to varying concentrations of FC-133 (lot-37). E(EEnv&. PLCab-) Div. AQquueaiteiyc) S REPORT AT BaSCToRmApCaTN n:yR(R2O:0wo0rdi-lTh2iss5ose0ct inforismdarteidobny he Technical Commanications Center The for afcauttheeadstmaitnincowasqua(tPiicme9p6h-ahleosur pLrCohselvaas)luefsololbotwaiinnged e7x7p6o(s7u4r3e-81t2o) FCa-n1d43754in (723-78re7pl)icatmedg/elx.perTihmeentcsomwbeirneed 96- hour LCgo was 766 (743-789) mg/l. Dlaitniatsg.enerated include LCg, values with 95% confidence ~; Bacon 4. Welter IHntormEatiog RLi R 0 - 03782 concLusIONS 0 Ivnalutehseseforrepflaitchaetaeddmi9n6n-hoowusr (LPiCmsepahqaulaetsicprotmeesltas,s) twheerecal7c76ulaatnedd754 magr/el.conMsaitdeerrieadlstopobessepsrsaicntgicaanllyLCsnponOtFoxitchisbaosredderonofthmeagNnIiOtSuHde criteria for aquatic toxicity interpretation. (2) INTRODUCTION: Trhoeomtetsetmpesruabtsutraen.cesTeisstsa wpheirtfeormfeidneopnowtdheirs, msaotleurbilael einvawlautaetredatits pofotetnhtiisalrepaoqruta.tic toxicity and the data generated form the basis ACCLIMATION AND EXPERIMENTAL DESIGH: TdheescrtiebsetdpbryotoUcSoElPAut(i1l9i75z)e.d f(o1)r this study was modeled after that Tphreivaftaethehaadtcmhienrnyow(sa). (SPitmeophcalkesFisphrowmeerleash)eldwerien oabtfalionwedthfrroougmha swyastteermatfib1e4-r1g6lasdsegrheoledsinC.g taAnkdaifliyllepdhowtiotpherciaordboonf-f1i6ltheoruerds wleilglh-t maandint8aihnouerds tdharroku,ghwoiutthtahe3a0c-cmilniumtaetiotnranasnidtitoenstpienrgiopderiwoads. The pfirsihorwetroeanfedd tThertoruag-hMoiunt t(bh)e dtaeislty,perfiooodd.beiFnigshwiwtehrheeladccl48imahtoeudrsto test temperature for 2 days prior to use in assays. Tcwheemnitcyal(.20) Alfla-tghleaasdsmianqnuoawrisa,, u3n5ixf2o0rxm25incsmizceo,ntwaeirneingtes1t6edliatterseaocfh drialnudeonmltyplaulsloctaotxeidcantto ecaocmhpritseesdt cahasmtbuedry wcihtamhbienr.30 Tmeisntutefsish were following toxicant addition. The fathead minnow experiments were cooxnydguecnteldevealt, 1a9n+d2 pdiegrOefesalCl. teMsotrtsaolliuttyi,onstewmepreeratmueraes,uredidssaotlved o2b4s-ehoruvratiionntserwvaelrse orrecuonrtdield rtoetlaaltivmeorttaolistwyimmhaidngocpcautrtreerdn. andGeneral behavioral changes induced by exposure to the test chemical. Tuhseingacuctaerbosnh-ofritl-tteerremdswtealtlicwabtieorasOsfaysknwoewrnecopmeprofsorimteidononandFC-143 constant quality as the diluent (Tables 1,2). Tfhoello9w6i-nhgourFC-f1i4s3h sltougdaireisthwmeircecopnecrefnotrrmaetdioinns:repl5i6c0,ate650u,sin7g50,the870, and 1,000 mg/1. pLrCsogbitvalpureosgrwaimthand95%34coTnRfAiCdeCnocmeputleirmitssystweemr.e calculated using the J Monitoring equipment used tenperature--compensating iplnlcmleutdeerd,ASaTMYeltlhoewrmoSmpertienrgss, dOirsisoonlved oxygen meter, and a Taylor temperature chart recorder. a) b) Dale Pattig Fish Farm, Brady, Commercial Fish Food of known Nebraska. composition. i 03783 RESULTS: . 3 TRhaed afnathaevaedramgiennwowes i(PgOiFmhe3pt-h4algersanpsromaenldasa) luesnegdthinOfth3e.6sechs.tudiTehse lfoaacdtionrg isfac0t.o8rgw/a1s (01)..5 g/T1e;mpetrhaetumraex,imupmH, reacndomdmiesnsdoeldveldoadoixnyggen 7v.a9lue-s8.a0ndplrianugneists,obtaandine9d6-dhuoruirngdistshioslvestdudoyxywgeerne:valu19e4s+ 1r0anCg.e,d mirnoimma4l.0ox-yg5e.0n msga/tlu.ratTihoenseof l4a5t-t5e6r%.values calculated out to a aInndth7i5s4 rmge/pll.icaCtheedmisctuadlys, ptohsesefsissihng9a6n-hoLuCrsoLCvsagjuevaliunesthwiesreran7g7e6 earnevircoonnmsiednetre(d2).to be practically nontoxic to the aquatic The data reported herein are based on studies performed by M.T. Eclonpaybaorfawtyh.is ArcecpoomrtpaniysinfgileddatainshtheeetsEncvoimrpornimseentaolrigLianbaolratdoartay. A Acchive System, Building 2-3E. tItheshaobusledncebeofnotiendfotrhmaattiotnhergeelnaetriavteedtoretshuelt"spuwreirtey"reapndorttehde in "rsetpaobritleidtyL"CsofvatlheueschewmeircealcalsucbusattaendceonFCt-h1e43bas(iLsotof37)t.he T"hoebserved mcoorntcaelnittryatiraotness*ofanFdC-1i4n3th(eLotabs3e7)nceinoftetshtesaocltuutailonsm.easured final y REFERENCES: (1) UMaSc/rEoPiAnv-erMteetbhordastesforanAdcuAtmephTiboixaincsi,tyETceosltosgiwciatlhRFeisseha,rch Series 660/3=75-009, 1975. (2) ERdeigtiisotnr,y oCfhrTiosxtiecnseEnf,fecfit.sEo.f, CahndemiEc.alJ.SuFbasitracnhcielsd,, E'd7s6., p. XV aantdioCnia,l U.ISn.stDietputaertmfeonrtOcocfupHaetalitohn,al EdSuacfaettiyonandandHeWaelltfha,re; Washington, D.C. (June, 1976). J 03784 TABLE 1 D WELL WATER QUALITY SSAAMMPPLLEE TDAAKTEEN:: 4/33M0/B8l0dg. 2-3E SAMPLE DATE 4/30/80 (TAKEN FROM TANK #4) PARAMETER CONCENTRATION EWnAvT.ERLQasUALITY BOOK REF. pRietsidual Chlorine ACmomnodnuicatiNviittryogen ATloktaallinSiotlyids TToottaall HFalrudonreisdse TDoitlaulteCCoOlDiform Standard Plate Count 70..602 mg/1 89 4<000.1 ummgh/ols/acsn Nat 25C is9 232768 mmgg//11 as CaC03 88 207,202mg/mlg/1as CacoO3 89 n1.e3gatmigv/el BE]10 42 per ml 10 AArlsuemniincumasasAsA*L 00..00105mg/mlg/1 114 | CBoobraolntaassBCo* 0<0..0202mg/mlg/1 114 CCohprpoemriumasasCu*Cr* 00..601085mmgg//L1 11aa CIarodnmiuasm Faes*Ca* 00..002022mgm/g1/1 11a4 NLiecadkelasasPb*Ni* 00..000055mgm/g1/1 114a SZiilncverasaszn*Ag* 00.,10302ng/m1g'/l 11aa Hercury as Hg* <0.2 ug/l 1a pCBIs** <0.50 ug/1 36 TotPaelstOircigdaensochlorine** = <0.50 ug/l 36 TOortgaalnophosphoru*s* 0.50 ug/1 36 Pesticides ***SSaammppllee DDaattee 32//3216//8800 J COC78S . TABLE 2 : . : ENVIRONMENTAL LFAIBROSRTATQOURAYR,TERBLLYDG.REP2O-R3TE,, S1T9.80PAUL, MINNESOTA WELL WATER SUPPLY FOR AQUATIC TOXICITY TESTING S ope ----eoe e--H EP t mme rSmear PARAMETER . Jan24uary| Febr2u6ary| Ma3r1ch| AV(E+RSA)GE TOasTAmLg/HlARDCNaEcSoS3* TOaTsAmLg/"1M" CAacLoK3ALINITY* 223 220 ELasECuTmRhIoCsALpSerPEcCmI?FICatCO2N5DUCCTANCE* TOasTAmLgS/1OLIDS* 350 264 + 4 223 +3 383 + 15 387 + 32 STANDARD PLATE COUNT** per 1.0 ml 19 26 + 6 - CRITERIA OF CONSTANT QUALITY * M#o10n,thlyArSaTnMgeCsommsihtotueled bEe-35lesosn tPehsatnic1i0d%eso)f. their respective averages. (Draf ** MtotneteRh3l5y roanngPeestoifcipdHess)h.ould be less than 0.4 unit. (Draft #10, ASTM Commi: "* T(oUtSaElPAb-a6c0t0e/r8i-a7l8-0d1e7n,sitpiaegse s10h1o.u)ld not be greater than 500 organisms per ml. 2 03786