Document e15050K1rNmrENpwEneOBDE9G

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredto as PFOS, FC-95, or as a component of FC-3017. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-3017 produced inAntwerp, Belgium. CurrentinformationindicatesFC3017 isa mixtureof0.67% PFOS, 20% diethyleneglycolbutylether,55.41% water,21% ethyleneglycol,1.33% Hydroxy foamer, 1% sodium octylsulfate, 0.5% polyoxyethylenemonooctylphenylether,0.04% sodium laurylsulfate, and 0.05% tolyltraizole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately retlecttoxicityof the tluorochemical component of the testsample. METHOD: Method: OECD Guideline202 Type: Acute static GLP: No Year completed: 1995 Species: Daphnia magna Supplier:In-houseculturesm,aintainedatAScl-DETD, Duluth,MN Analyticalmonitoring:Temperature,pH, conductivitayn,d dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old Statisticamlethods: LC50 values calculatedusing a computer program. Test conditions: Dilutionwater: DechlorinatedCityof Duluth,MN tap water post carbon filteare,ratedfor24 hours priorto use. Dilutionwater chemistry: Hardness: 50 mg/L as CaC03 Alkalinity: 46 mg/L as CaC03 Conductivity: 127 - 143 pmhos/cm pH: 7.82 TOC: 1.6mg/L Lighting:Cool-whitefluorescentlights.A dailyphotoperiodof 16 hours light and 8 hours darkwas maintainedthroughoutthetestingperiod. Stock and testsolutionpreparation: The testmaterialwas added directly tothe dilutiownater on a weight/volumebasisand mixed by mechanical stirrinagtambient labtemperature. Exposurevessels:250 mL beakerswith200 mL testsolution. Number of replicates4: Number ofdaphnids per replicate5: Number ofconcentrations:one plusa blankcontrol Water chemistryduringthe study: pH range (0-48hours): 7.9- 8.2(controelxposure) 7.8- 7.9(1000 mg/L exposure) Temperature range (0-48hours): 20.1 - 20.6 'C Conductivity range (0-48hours): 127 - 139 pmhos/cm (controelxposure) 142 - 143 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-48hours): 8.1 - 8.8 mg/L (controlexposure) 7.7- 8.9mg/L (1000 mg/L exposure) RESULTS Nominal concentrations:Blank controland 1000 mg/L. Element values: 24-hour EC50 = >1000mg/L 48-hour EC5o = >1000 mg/L 48-hour NOEC = 1000 mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas described inthe Test Substance Remarks field.The values reportedapply to thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 48-hour EC50 was determined to be >1 000 mg/L. The 48hour No Observed EffectConcentration(NOEC) was 1000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking = 2. This study meets the criteriafor quality testing.However, sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmationofthe amount of fluorochemicalproportionin solution. REFERENCES Thistestwas conductedby ASci CorporationD,uluthEnvironmentalTesting DivisionD,uluth,MN attherequestofthe3M Company, Lab Request number N3188,1995. OTHER Lastchanged: 6/26/00. C@@rl@rati,@n/ASD%u:lluth Etivir,)ntitTcen.t-..iIiiDnigvi%ii)n ASci Stt,J.I*D9 5010-Co6g STUDY TITLE ACUTE TOXICITY OF FC-3017 TO DAPIIVI.4.1f.4G,%'-l DATASTANDARD OECD GUIDELINE 202 STUDY CONIPLETED December 23, 1995 TESTING FACII-ITI' ASCI Corporation/ASCI-Duluth Environmental Testin0c,Division 112 East Second Street Duluth, NIN 55805-1701 Tel.No. (218)7224040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUNIBERS ASci Study ID# 5010-068 3NI Company Lab Request# N3188 Sponsor: 3M Company Sponsor Study ID# N3188 Envin)nment;ilTestingDivisii,n AScl Study ID# 5010-068 1.0GENERAL INFOR-I%IATION StudyTitle Data Standard Sponsor Sponsor's Representative Sponsor'sLab Request# TestingFacility PrincipalInvestigator ProjectDirector TestingFacilitDyirector DefinitivTeest Dates Data Validationand ReportReview Acute Toxicityof FC-3017 LightWater Brand Aqueous Film Forming Foam to Daphnia magna. OECD Guideline1.02. 3M EnvironmentalTechnology and Services, Buildin0c2,-3E-09,935 Bush LAvenue. St. Paul. NIN 55106. Susan A. Beach 3,%tEnvironmental Technology and'Services,Buildinc2,-3E-09, 935 Bush Avenue, St.Paul,NIN 55106. Tel No. (612)778-7452. N3188. ASci Cori)oration,'AScl-DultEint@l,iironiiietital TestingDivision,112 EastSecond Street. Duluth,NIN 55805; Tel.No. (218)722-4040. Joe Dierkes. Joe Amato. Donald Niount. December 21-23, 1995. Alan Niozol. ePO rtSignature- Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport Two yearsfrom the datestudy iscompleted. AScl Corporation/AScl-DulutEhnvironmental LestingDivision,112 EastSecond Street, Duluth,MN 55805; Tel.No. ('.1187)22-4G40. Sponsor: 3M Company Sponsor Study ID# N3 188 2 Duluth Envin)nmcniaTlc.%tinDgivision AScl StudyID# 5010-068 '-.O0BJECTIVE(S) To determintehe48-hmedianeffecctoncentrati(oEnC5,a)nd48-hno observableeffect concentratio(nNOEC) of thetestsubstance,FC-3017 LightWater Aqueous Film Forming Foam, forDaphnia niagna. This studywas conductedaccordingtothe AScl studyplartretainedbv theSponsor and OECD Guideline202 (OECD 1993). TestSubstance Test Organisms DilutionWater Exposure Chambers 3.0 TEST NIETIIODS Properties:Clearamber-coloredliquid,specifigcravitncia. 1.1 (water 1),misciblewith water,and stable. Tt,.cvotticetitratio0ti(vd:ilutiownater control)aiid1000 HiCL-,h concentratiotnestsolutionwas preparedby mass additionfollowedbv mechanicalstiffinagt ambient lab temperature. Source: Healthy,lessthan 24-h old Daphnia magna neonatesobtainedfrom AScl stockculturesmaintainedin water of testdilutiownaterquality. Use in tesi:Gravid adultsisolateidndilutionwater24 h beforethetest initiatioUns.ed 5 organisms/replicataend 4 replicates/tecsotncentration. Handled witha smooth bore pipetwithan insidediameterof 6 mm. Did not feedduring thetest. Source: DechlorinateCdityof Duluth,MN, tapwater.The most recent chemicalanalysisisin Raw Data Package. CliaraciefisticAst: testtime,thewater had a hardnessof 50 mg/L (as CaC03) and a pH of 7.82.Aerated for24 h priorto usingin thetest. 7ype: 250-ml borosilicatgelassbeakers. Use in test:200 ml of dilutionwater or testsolution/chamber.During the test,keptcovered,except when experimentalobservationswere made. Sponsor: 3M Company Sponsor Study ID# N3 188 3 Incubation Observations Endpoint Calculations A.S@:C1tvrT,,ir-A,t'nAS@I-Duluth EnvironmcntaTlc.%tinDgivision AS4.1StudyID# 5010-069 Duration:48 h. Dailyphoroperiod1:6h lighatnd8 h dark,maintaineudsingcoolwhite fluorescentlamps. Teniperature:20'C. Biological:Daily- gentlyagitatedtheexposure chambers and then countedimmobilizedand dead testorganisms(immobilizedorganismswere thosethatdid not swim within 15 seconds afterexposurechambers were gentlyagitated).Did notremove any daphnidsfrom theexposurechambers at 24 h. IVutercheniistry:(1)At testinitiationt-otalhardness,alkalinitys,pecific conductivityp,H, and DO, (2)dailv-teniperaturaen,d (3)attest terminations-pecificonducti-,,iptH@.,,and DO. All determinations%,cre made accordingtoAPHA methods (1989). Formal calculationnsot needed due to lackof effect. 4.0 RESULTS Test SubstanceToxicity(Pertinendtataare in Table 1) 24-h EC@,,: > 1000 mg/L. 48-h EC_,,,>: 1000 mg/L. 48-h NOEC- 1000 mg/L. Sponsor. 3M Company SponsorSubdyID# N3199 4 AS@:1C,)r;-.3rjli,)n!ASCI-Duluth En%ir,,nmcntalTc%ting Divisitin A'-@LlStudy IL)#50]G-068 RangesofWaterChemistrPyarameters Totalhardnes(sasCACO.,nkg/L):50. Alk-alin(iarsvCACO,nigIL):46. Teniperatt(i*rCe):20.1-20.6. DO (tikq/L7).:7-8.9. pH: 7.82-8.20. i Specificotutuctit-(itrivniht)slc@1p2i7)-:143. -i.0TEST QA/QC QA/QC Criteria Test organism dead, immobilizedor trappedin controlexposuremust not be more than 10%. Data O@,'dcead. immobilized or trapped. The lowestDO concentratioant test terminationmust be at least60% of theair saturatiovnalueat testtemperature. The lowestDO concentratiomneasured was 85% of the airsaturation%-alueat 20.OOC. Test durationmust be 48 h. The testdurationwas 48 h. Referencesubstance(sodium chloride)toxicity AScl's updatedreferencetoxicittyestcontrol test. chartisintheRaw DataPackage._ 6.0 REFERENCES American PublicHealthAssociatio(nAPHA). 1989.StandardMethods fortheExaminationof Water and Wastewater.APHA, Washington,D.C. OrganizationforEconomic Cooperationand Development (OECD). 1981/1993.OECD GuidelinesforTestingof Chemicals.OECD PublicationInformationCenter,Washington,D.C. Sponsor. 3M Company Sponsor Study IDO N3 188 5 AS@i Cairiu@r:-tit@n,A.Sci-Duluth Envirt)nmcnt;iTlcl.LingDivi.%ion ASci Study ID# 5010-068 TABLE 1. Acute toxicityof FC-3017 Light Water Aqueous Film Forming Foam to Daphnia magna TestSubstance Concentration (mgtl) 0 1000 Percentagesare inparenthesis. CumulativeNumber and % of Dead and ImmobilizedOrganisTmsl 24 h 48 h 0(0) 0(0)- 0(0) 0(0) EFRECT AND!';O-Ef.7ECT CONCENTRATIONS 1$*'ITII CORRESPO,NDING 95@/ZCONFIDENCE 1.%TERVALS EC, and NOEC 24-h EC, 48-h EC, 48-h NOEC Data(mi:L,) > 1000 MI!,;L > 1000 mt!!L 100-Ome/L 95% Confidcncelnic.-al Not calculable Not calculable NotaEE@@@ Sponsor: 3M Company SponsorStudyIDN N31U 6