Document drdJaREDvjLo7JZoXkQk55w6

6620 TtiftonAPU Road L ounger aboratories St. Louis as>, Missouni BlocUntiiti ... PlvVmaa>l*><?uU ... AaahfiU H PBON,, TM.OIH 6.aB*0 SUBJECT - Toxioologioal Investigation Oft Aroclor 1260 Monsanto Sample Number 39 Monsanto Project Number 1-62-37 June 2$th, 1962 The data, together with the dilution at vhioh the ooapeund was fed, are shown in Table I. B) Skin /beorptlon XU) (Babbits) The diluted conpound vae applied in Increasing doses at Increments of 0.2 fractional leg Intervale to the closely clipped. Intact skin of New Zealand whits female rabbits. . The treated areas were covered with plastlo strips and the animals plaoed In wooden stocks for periods up to twenty-four hours, after which time they were assigned to individual cages. Observations were made for tcoclo symptoms and the risesr. of the animals that suoeimbed were examined maoroeooploally. The data, together with the dilution at which the compound was applied, are shown In Table 11. MONS 035060 TU. confident].1 report i. sot to b. urnd In nny form of mlvortUin, without written pnrmiteioo Toi Monsanto Cfcoadoal Oy juetf st, Louis, Missouri Tounfsr Laboratories Oortlfleate of Amlyala - Pas* 2 (6/25/62) - r-62J7 SDHMART /pooler 12(0 A) Oral U)i0 (Rato) nw Oral LDo ibr ntl* and fanala rato mi pUoad at 10,000 *1111grans par kilogra* vith lousr and upper Halt* of #,600 to 11,600 *llllgrms cor kllogr**. ^ Ttm eaapound vao olaoaod a* praatleolljr noo-taaie by oral irurastlon la aala and faaala rata. B) Skin Absorption JfcE (Rabbita) llie Minium Lethal loo* by Skin Abaorptlon in fiaaala rabbit* was found to bo grantor than 1260 alUigrma par kllncra* and lao* than 2000 nilllfn** par kllotraa, Tfaa ooapound Mas elasnad a* alldly toal* tgr akin absorption la fmaals rabblta. rowan labor/vcrbs HONS 035061 To I Monssnto Qwmlcal Cv .pnjr St. Louis, Missouri lounger Leboratoriee Certificate of Analyeie - Peg* 3 (6/25/62) - T-62-J7 TABLE I ') THE ORAL LD,0 OF 'Aroolor 1260' FOR RATS Semple Fed As A 50,Of Solution to Core Oil Animal No. - Sex 1- Female 2- Female 3- Fatale it- Male 5- Male 6- Male 7- Female 8- Female 9- Female 10* Male 11- Male 12- Male 13- Female lit- Female 15- Fenale 16- Mato 17- Male 16- MdLe 19- Female 20- Foatale 21- Male . Weight Om. 21? 230 220 2lt? 23? 2?0 220 210 21? 2k0 22? 23? 210 22$ 210 230 23? 220 210 21? 240 Doee Hg./Kg. 6,310 6,310 6,310 6,310 6,310 7,940 7,940 7,9itO 7,940 7,940 10,000 10,000 10,000 10,000 10,000 10,000 12,600 12,600 12,600 12,600 12,600 Fate Survived Survived Survived Survived Survived Survived Survived Survived Died Survived Died Survived Survived Died Died Survived Died Died Survived Died Died Discussion - The Oral LO,0 for male and female rate vaa placed at 10,000 miUigrema per kilogram with tower and upper limit* of 8,600 to 11,600 milligram* per kilogram. The compound w* olaa*ed a* poetically nom-tode by oral Ingestion In male and female rat*. Survival time waa two to five day* with most death* occurring In three days, Toxlo symptom* Included severe diarrhea, loss of appetite, and Increasing lethargy and weakness. At autopsy there was inflammation of the gastrlo mucosa and liver hyperemia maorosooplcally. HONS 035062 To i Monsanto Chenloal C. pany St, Lovett, Missouri Toung*r Laboratories Oartlfleata of Analyal* Pag* It (6/25/62) - 1-62-37 TABLE II THE ffiNMJK LETHAL PC8E CP 'Aroclor 1260' BT 3Km ABSORPTICI? HI RABBITS Sample Applied As A SO. Of Solution In Corn CHI Animal No, - Sex Weight JJL:. Doee g. / Kg. Weight Change 5 Daya Later _____ Kg._____ 1 - Female 1.9 SCO. 2 - Female 2.2 791* 3 - Female 2.1) 1260 It - Female 2.6 2000 5 - Female 2.1) 2000 6 - Female 2.1) 3160 0.1 0.0 0.0 - 0.1* - 0.1* * 0.5 Fete Surrited Survitad Surrltad Died -- 8 Daya Died -- 7 Daya Died -- 7 Days DISCISSION - Tha Hlnlmua Lethal Doee by skin Abaorptlon In feula rabbit* vu found to be greater than 1260 milligram* per Idlograa and lee* then 2000 milligram* per kilogram. The oompound was classed a* mildly tcado by akin abaorptlon In female rabbit*. Surrital tin* was eaten to eight daya, Toclo symptoms lnoludtd weight loea after two to three day* with Increeling weakneaa followed by oollapae In alx to eeren daya. At autopay there waa erldenoe of liter dyafwnoticm and renal hypemaia maeroaooploally. HONS 03S063