Document dnv4eZZjEvwKJNJd61znDaGaQ

| sumrTep By: PREPARED BY: | AppROVED BY: J EG&G, bionomics AquaWtairech7a5Tn0Jo,uxniHeMca,aoinslos1ga9Syc7i8hrueLseaetbtotrsatory brenda F. Wilson Aquatic Biologist Stephen 3. Fils i 4 Lin George A. Cary Director, Aquatis Biology 03772 ey 3 SUMMARY OF HISTOPATHOLOGICAL EXAMINATIONS OF FATHEAD MINNOW (inephales promelas) EXPOSED TO 78.03 FOR 30 DAYS. Fe-ryz RESEARCH REPORT ) SUBMITTED TO 34 COMPANY ST. PAUL, MINNESOTA REPORT B-78-9-301 EGsv4BMG,ITBTiEoDnoBmYics AquWaatriech7aT9m0o,xMiaMciaosnlsoagScythrueLseaetbtotrsatory , Septnber, 1978 03773 D vrropucrion An "egg and fry test" was conducted between March 31 and May 4, 1978 (Bionomics Report #BW-78-6-175, June, 1978) to assess the sub-lethal effects of 78.03 on fathead minnows (Pimephales promelas). Effects on egg hatchability and on survival and growth of fry were measured. To further investigate possible effects due to exposure to 78.03, histopathological examinations of exposed fish were also performed. The results of these examinations are reported here. MATERIALS AND METHODS ) Upon terminati; on of the e5 gg and fry study, fi: ve fish from each replicate of the high 78.03 concentration (100 mg/g, nominal) and control were preserved in 10% buffered formalin and sent to the Environmental Pathology Laboratories, Inc. Carolina, Rhode Island. The fish were examined for gross lesions, and sagital sections were prepared by a pathologist. Fish were placed with indentifying numbers into processing cassettes, dehydrated, cleared and infiltrated on an Auto-technicon tissue processor using the method of the Armed Forces Institute of Pathology. The J) fish vere then placed in a vacuum oven and subsequently embedded in paraffin. All blocks were sectioned at 6 microns: one 03774 N 2 slide was prepared from each block and stained with hematoxylin and eosin. Special staining procedures such as PAS, Trichrome and Acid Fast were used occasionally when requested by the pathologist. All blocks were sealed with paraffin and stored. Slides were labeled, boxed and delivered with all work sheets to the pathologist for examination. Two slides were prepared from each fish. Tissues processed and examined included but were not limited to; gill, thymus, liver, spleen, heart, gonad, kidney, foregut, hindgut, olfactory mucosa (nares), brain, ear, skin, muscle, pancreas and pharyngeal mucosa. mesure i A summary of the examination of each fish is reported in Table 1. Only those tissues which were missing or contained demonstrable change are listed. The only tissue changes observed were hyperplasia of gill lamellar epithilium and fatty change of the liver. These changes were judged to be minimal and consistent with changes seen routinely in healthy fish. Autolipis of gill tissue was observed in several fish. This change was probably due to the poor penetration of the buffered formalin to the pos- terior dorsal portion of the gill space. O3775 3 D Based on these data, it is concluded that 30 days exposure to a nominal 78.01 concentration of 20 ug/? did not cause any significant, demonstrable, tissue changes in 30 day old fathead minnow fry. J) CO3776 ) mable 1 -- Histopathological examination of fathead minnow (Rimephales promelas) exposed 30 days to 100 mg/s nominal 78.03 and control water. 100 mg/s 78.03 Fish # sex 1 ukn 2 Ukn 3 Ukn )y - 4 kn 5 6 F |1 : 7 F 8 F 9 Ukn 10 F J ocrongdaintion MTiissssiuneg Normal Normal Normal Normal Normal Lcihvaenrg-eFaItcty G(ielpli-tHheylpieurmp)lasIia Lchiavnegre-FaItty G(ielpli-tHhyelpieurmp)lasIia cLhiavnegre-FaItty Lchiavnegre-FaItty Lchiavnegre-FaItty Snaprleese,n, egaornad, Stphlyemeuns, gonad, nHeaarrets, gonad, Stphlyemeuns, gonad, Spleen, nares Nares, thymus Nares, ear Spleen, ear Snpalreeesn,, `egaornad, Spleen, nares C0377? Table 1 (cont.) ) _-- Fish Sex -- coonrdgiatnion TMiissssuien,g ee Control 1 kn \! 2 Ukn 3 F 4 F ) 5 ud a 6 F 7 Ukn |: 8 vkn 9 r 10 Ukn -_-- aFenale. bUnknown. cLesions are minimal. Ja Mass Lcihvaenrg-era1tty Gtohnyamdu,s nares, G(ielpli-thhyelpieurmp)lasIia , cLhiavnegre-FaItty nSaprleeesn, gonad, G(ielpli-thheylpieurmp)lasIia Thymus Normal Spleen, nares Normal hSepaleretn, thymus, Lcihvaenrge-FaItty Spleen, heart . Lcihvaenrg-eFaItty Sgpolneaedn,, "thheyamruts,, nares Lcihvaenrg-eFaTtty nSaprleese,n, tghoynmauds, Lchiavnegre-FaItty Spleen, nares G(ielplithheylpieurmp)las1 ia Normal Gonad, thymus CO3778 Sra ) comurreep By: PREPARED BY: APPROVED BY: J AquatiEcGTo&xiG,colBoigoynomLiacbsoratory 790 Main Street WareSheapmt,embMears,sac1h9u7s8etts Stephen J. Ells 4 Frcsooromiat George A. Cary Director, = Co3779