Document dnkNk9MgOJ31zbv7DQQDBLER0

59105 Chemical Abstracts Vot. 71, 1969 > p#s 1'-' was mixed with diphtheria toxin (0,2 ml.) and incubated with antitoxin at 37" for 5G days in medium 190. The min. toxin concn. detd. by the metabolic pll-indicator test using phenol red was lO'MO'1 Id/ml. The test was applied using a range of in oculum sizes, vol;., llCOj- concn, and antitoxin conrn. Vari ance was 20% compar'd with 7% in the cytopathogenicity test, but the method was easier and was suitable for serial diln. ap plication. Peter M. Motion 59105g Toxicological investigation of cyanides, Ortega, Manuel; Vallejo, K. (Inst. Nac, Toxicol., Madrid, Spain). An. Real Acad. Farm. I960, 32(4-5), 470-87 (Span), blood (1-2 ml.) or minced visccia (2-3 g.) was placed in a Conway cell for 4 hrs. with 4-5 drops of 10% H;S04 and ItCN evolved was absorbed in 0.1 AT NaOll. To the la'ter were added 2 ml. J/ Na- HiPOi and 1 ml. 0.25% chloraminc-T, then after 2-3 min., 3 tnl. reagent (3 barbituric acid, 15 ml. pyridine, 3 ml. HC1, and water to 50 ml.). The red to reddish purple color was read at 580 mu after 10 min. Beer's law was obeyed for 0.5-2 y KCN/ml. With time, cadaver CN~ is converted to CMS". To det. this, blood or viscera was dcprotcinizcd by adding an equal amt. of Hj- POi, heating to boiling for 10 min., and adding 50 mg. of powd. picric acid/g, of tissue. After 12-24 hrs. at room temp., it was filtered and 1/3 vol. 5% K-CrjOj and 2/3 vol. 50% H;SO were added to the filtrate in a Conway cell. Detn, of CN" was as above. Recovery of added CMS- was 54% (7% if not depro- teiniicd). Disappearance of CN postmortem is approx, ex potential and the amt, present at death cannot be accurately ealed. from subsequent detns. J. R. Gwilt 59106h Identification of hashish (Cannabis sativa). Cham- bon, Paul (Fac. Med. Pharm. Lyon, Lyons, Fr.). Bull. Trav. Soc. Pharm. Lyon 1968, 12(1), 43-G (Fr), A sample of hashish was identified using color reactions and thin-layer chroinatog. on silica gel G. The principal constituents were cannabinol, cannabidiol, and tetrahydrocannabinol. Dorothy J. Buchanan-Davidson '59107j Identification and determination of nonvolatile or ganic poisons by infrared spectroscopy in forensic toxicology. Cor- neteau, II.; Monnet, R.; Boitcau, H. L. (Fac. Mixte Med. Pharm. Nantes, Nantes, Fr.). Med. Leg. Domm. Corpor. 1968, 1(4), 352-9 (Fr). Klcven alkaloids and 9 neuroleptic agents were identified and cstd. by ir spectrometry after extn. and sepn. by thin-layer chroinatog. on silica gel. The chromatogram elu- ates were coned, on KBr micropellets. Spots centg. 10 y of compd. could be estd. with a precision of 8-15%. L. A. Dclicnnin 59108k Utilization of an anti--/-globulin immune serum for determining the human origin of blood stains. Tran Van Kv, Philippe; Lenoir, L.; Muller, P. (Inst. Med. Legale Lille, Lille, Fr.). Med. Leg. Domm. Corpor. 1968, 1(4), 392-5 (Fr). Extn. of a blood spot with isotonic phosphate buffer of pH 7.4 exts. the antigrn. Monospecific antisera are obtained from rab bits immunized with human yG-globulins. The immunologic tests are performed by double diffusion on gclose. L. A. Dehennin 59109m Evaluation of postmortem blood sugar levels and their correlation with the glycogen content of the liver. Ramu, M.; Robinson, Ann E.; Camps, F. E. Med. Sci. Law 1969, 9, 23-6 (Eng). Satisfactory blood glucose estns. may be made by using blood samples collected into tubes contg. F" up to 48 hrs. after death, provided ' hat a specific assay procedure is used. The blood glucose concn. ,:or samples from the left side of the heart and the upper and lower extremities statistically belong to 1 group whereas the right heart blood and the liver blood belong, statistically, to 2 different groups. There were no significant variations in the blood glucose concn. with the time interval 12- 48 hrs. after death. There is a significant inverse relation be tween liver blood glucose concns. and the glycogen content of the liver as assayed chem. Irving Sunshine 59110c Relations between the levels of alcohol in the blood and in the breath. Paolucci, G. Riv. Med. Aeronaut. Spaz. 1963, 31(3-4), 401-9 (Ital). Tests were made with 20 subjects (2 female) of av. age '*5 who consumed, in a single dose, 0.50 g. EtOH/kg. in the form of cognac. Exhaled air was collected every 15 min. up to 2 hrs. after ingestion and blood was sampled at 45 and 120 min. in 15 of the subjects and every 15 min. in the remaining 5. To det. whether differences existed between the air exhaled in 1 profound expiration and alveolar air, the latter was collected in equal vol. immediately after the former. The blood EtOII iucrcasid regularly up to 0`i min., then decreased iireruprV no to 2 ' TM p, p"/ g .:,]t m-d air to that in 1 ml. blood wax anouFl:2 tor the t'wxt nr. .liter in gestion, varying with the individual . Blood EtOH is detectable up to 2 hrs. in subjects with considerable adipose tissue but has practically disappeared after 1 hr. in lean subjects. J. I. M. Jones 59111f Fractionation analysis of metallic poisons. Krylova, A. N. Vopr. Sndebnoi Med., Min. Zhravookk.r. SSSR 1968, 314- 21 (Russ). From I<ef. Ah., Khim. 1969, Abstr. No. 2G175. A comparison of the accepted H;S method and the new fractiona tion method of analyzing human organs during autopsy is made, with relation to a group of 12 poisons (Pb, Ba, As, Eb, Bi, lie, Cu, Cd, Ag, Zn, Cr, and Mn). Qunl. and quant, anal, methods take 1-2 working days instead of the 8 required for the H:S method, with greater sensitivity and accuracy. MQRK 59112g Separation of copper and cadmium by extraction in forensic chemical investigations, Krylova, A. N. Vopr. Aui:b~ noi Med,, Min. Zhravookhr. S.SSR 1963, 322-7 (Russ). From Rcf. Zh., Khim, 1969, Abstr. No. 2G176. A method of extn. and complexomctric detn, of Cu and Cd in human organs is de scribed. Mineralize the sample with 1INOi-H;S04i add 20 ml. 20% K Na tartrate, 10 ml. glyceiol (1:10), and 30%, KOH until neutral and 5 mi. in excess. Add 10 ml. 1% Na dicthyldithio- carbamato (1) and 10-15 ml. CllClj, shake, and repeat the extn. until a colorless CHCb layci is obtained. Reext. Cd from the combined ext. by shaking with 10 tnl. N 1IC1 (3 times). Dil. the Cd ext. to 100 ml. with HiO and bring to pH 8. Titrate C(1 with 0.01 A' EDTA until the red-violet color changes to dark blue. Wash the CHClj ext. 3 times with 10 ml. 5N HC1 (to remove Bi and Tl) and then with 1I;0 until neutral reaction. Ext. Cu by shaking with 1% HgCh until colorless, and det. in the ext. by titrn. with EDTA in the presence of murexide and 1-2 g. KI at pH 8 (color change from yellow to violet). MQRK 59113h Biochemical modifications induced by acute and _ub- acute intoxication with white phosphorus in the rat. I. Action of repeated parenteral doses. Tmhaut, Rene; Claude, Jean R.; Warnct, Jean M. (Fac. Fharm., Paris, Fr.). Ann. Pharm. Fr. 1969, 27(1), 17-23 (Fr). Repeated administration of white P to rats, parenteTally, decreased body wt. A dose of 2.5 mg./kg. de creased liver wt. without visible steatosis; liver triglycerides were increased and-phospholipids decreased, leaving the total lipid content nearly const. In the blood both total lipids and tri glycerides were lowered. With 5 mg./kg. several rats died after 7-8 days following the 3rd injection, and all showed varying de grees of liver steatosis with increase in triglycerides and in total lipids. Since the activity of glucosc-6-phosphatc dehydrogenase remained normal it is unlikely that the biosynthesis of fatty acids and triglycerides was increased. Ruth M. Chester 59114j Toxicity of pyrolysis products of vinyl plastics. Corn ish, Herbert H.; Abar, Ellen L. (Seh. of Public Health, Ann Arbor, Mich.), Arch. Environ. Health 1969, 19(1), 15-21 (Eng). Po!y(vinyl chloride) (I) and vinyl chloride-vinyl acetate poly mers (II) were pyrolyzed in air by gradually raising the air temp, from ambient to G9U". Rats were exposed to the pyrolysis prod ucts air stream dild. with room air to twice its initial vol. Ex posure to air contg. the pyrolyzed products front 1-2 g. I resulted in death to 50% of the animals. Most deaths were due to CO. and carboxyhcntoglobin levels correlated well with the amt. of pyrolyzed I. Little histol. evidence of lung damage was evident. When the air stream was dild, with O, pulmonary edema and in terstitial hemorrhage developed. The lungs of animals exposed to high levels of II pyrolysis products showed focal edema and intraalveolar hemorrhage. I formulations contg. additives and inert materials were generally less toxic per g. of sample pyro lyzed. BZJN 59115k Validity of a critical blood level for prevention of di- eldrin intoxication. Keane, William T., Jr.; Zavon, Mitchell R. (Agr. Chem. Div., Shell Chem. Co., New York, N.Y.). Arch. Environ. Health 1969. 19(1), 36-44 (Eng). In dogs given a single dose of 5 mg,/'kg. of dieldrin, dieldrin concn. in the blood rciched a max. in 2-4 hrs. and declined after 10 hrs. to a relatively const, level which persisted for the foliowing 22 hrs. Ail intoxications should therefore occur within 2-4 hrs. after feeding dieldrin. In dogs given 1.0 mg./kg. of dieldrin for 5 days, 0.2 mg./kg. for the next 57 days, and then 2 mg./kg. daily until intoxication occurred, the 1st muscular spasm occurred at a baseline concn. of 55 fig./100 ml. of whole blood. In both acute and subacute in toxication, the baseline concn. prior to intoxication was 62 ng./ 100 ml. After administration of the daily dose and subsequent appearance of intoxication, the blood concns. of dieldrin were, resp., 74 and 83 ng./100 ml. blood in the acute and subacute groups. The difference was not significant. Correlation be tween dieldrin concns. in blood and body fat was 0.84. The re sults support the hypothesis of a threshold level for dieldrin in blood which, when exceeded, results in intoxication indepen dently of the type or duration of exposure. Raymond Zchnpfentiig 59116m Alteration of the locomotor activity of mice poisoned with Gublethal doses of lead acetate. Terzin, A. L.; Yu.'tov, V. V. (Med. Fac., Novi Sad, Yugoslavia). Ark. U;g. T-~- !5'.S. 1PM1, 4'vl-5]l 'li-TM. Inhett.m ...` i.A-.L'j (0.4-20 n,g. i.p.) into female mice '21-33 v.' pr-Mve-d O' .-e-de pendent changes in locomotor activity. Single doses r udneed depression of locomotor activity while chronic poronin.; i ici a-vd locomotor activity. Single or multiple i.p. injections of .-a'ano did not alter locomotor activity. Animals poi.-oned win lend doses of Pb(OAc)j developed stippled Cells during the 2nd or dru week while significant changes in locomotor activity were ex'd-.iu 1 day after the last injection. Mice poisoned with sub'vtl at doves (Q.4-1.0 mg.) showed no stippled cells but display d eh;."., e-* m locomotor activity. F. H. Re iferu 0L1 7354