Document dnbkN0npxdDn41D5a0azvdyJq
TOXICITY TO AQUATIC PLANTS
TEST SUBSTANCE
Identity:Mixturecontainingperfluorooctanesulfonawtheich may alsobe referredtoas PFOS, FC-95, oras a component of FC-3003. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was notgiven.The testsample isFC-3003, identifiebdy the laboratoryas Rl 331. Currentinformation indicatesitisa mixtureof1% PFOS, 15% diethyleneglycolbutylether, 79.39% water,2% Sultonefoamer, 1.5% sodium octylsulfate1,% sodium decylsulfate0,.06% sodium laurylsulfatea,nd 0.05% benzotriazole.
The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluoruchemical component of the testsample.
METHOD:
Method: OECD Guideline201 Type: Acute Static GLP: No Year study performed: 1997 Species: Selenastrumcapficomutum Source: OriginalloybtainedfromATCC, RockvilleM,D, maintainedinculture medium atASci-DETD, Duluth,MN Element basis: Algalcellcounts (cells/mis)p,ecifigcrowth rates. Exposure period: 96 hours StatisticaMlethods: Statisticaanlalyseswere performed usingtheTrimmed Spearman-Karber method. Analyticalmonitoring: pH, conducfivitya,nd temperature
Test Conditions:
Algal NutrientMedium: Nutrientmedium per OECD. This nutrientmedium providedallmineralnutrientesssentialforalgalgrowth and alsoserved as the diluentforallalgaloperations.The pH ofthissynthetcalgalmedium was 8.0. Stock and test solution preparation: A 2000 mg/L primary stock solution was prepared by dissolving700 nigin350 mL algalmedium, followedby mechanical stirringE.ach testconcentrationwas made by volume additionof the stocktoalgalmedium. Exposure vessels: 250 mi Erlenmeyer flaskscontaining100 mi test solutions,ealedwithfoam stoppers. Agitation: 100 rpm. Number of replicates:3 forexposure concentrations6, forblankcontrol. Initiaclellloading: 1.0 X 104 cells/mL(1.3x 104 cells/mLactual).
Number ofconcentrationsf:iveplusa blankcontrol LightingC:ontinuoulsightiantg740 ft-candiuessingcool-whiftleuorescent
lamps. Water Chemistry:
pH range (0-96 hours): 8.00 - 9.90 (controlexposure) 4.72- 7.90(1000 nig/Lexposure)
Conductivityrange (0-96hours): 151 - 186 pmhos/cm (controelxposure) 159 - 170 pmhos/cm (1000 nig/Lexposure)
Test temperature range (0-96hours): 24.8- 25.8 OC (incubator)
RESULTS Nominal concentrations: Blank control,130,216, 360, 600, and 1000 mg/L
Algal Growth EC50 Values
Exposure (Contact)
Hours 24 48 72 96
Element
values:
Average Specific
Cell-Count
Growth Rate
mg/L (95% C.I.)
mg/L (95% C.I.)
233 (159- 342)
160 (150- 170)
<130 (NotCalculable)
251 (204- 309)
<130 (NotCalculable) 1 572 (434- 756) 1
<130 (NotCalculable) 1 64 (528 - 834)
96-hour
EC5o
<1 30 mg/L (celclount)
96-hour E,C5o 664 (528- 834)mg/L (growthrate)
96-hourNOEC = 23 mg/L (celclount) 96-hour NOEC = 96 mg/L (growthrate)
Element valuesare based on nominal concentrations.
Revemibilityof Growth InhibitionA:liquotsofthe 1000 mg/L testsolution were dilutedwithalgalmedium and culturedforan additionapleriod.Based on growth observed inthe recoveryphase, the effecton algalgrowth was
found tobe algistatic.
Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
FC-3003 exhibitsa 96-hour EC50 cellcount valueof <130 mg/L and a 96-hour E,C50growth ratevalue of664 (528 - 834) mg/L.
The 96-hourNo Observed EffectConcentration(NOEC) is23 mg/L forcell count and 96 mg/L forgrowth rate.Thistestsubstance was determined tobe algistatic.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
Reliability:Klimischranking= 2. Testingmeets the criterifaorquality testing.However, sample puritywas not properlycharacterizedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemicalproportioninthe solution.
REFERENCES
This testwas conducted by ASci Corporation,DuluthEnvironmentalTesting DivisionD,uluth,MN atthe requestofthe 3M Company, Lab Request number R1331,1997.
OTHER
Last changed: 6/26/00
ASci Corpomtion/ASci-Duluth Environmental TestingDivision
ASel Study [D# 5010-068
STUDY TITL GROWTH INHIBITION OF R1331 FOR GREEN ALGA (Selenastnocnapricomutwn)
DATA SIANDARD OECD GUIDELINE 201
STUDY COMPLETED April3, 1997
TESTING FACILITY AScl Corporation/AScI-Duluth EnvironmentalTestingDivision
4444 AirparkBoulevard Duluth,MN 55811
Tel.No. (218)722-4040 Fax No. (218)722-2592
STUDY IDENTIFICATION NUMBERS AScl Study ID# 5010-068
3M Company Lab Request#R1331
Sponsor: 3M Company Sponsor Study ID# R1331
AScl Corporation/AScl-Duluth Environmental TestingDivision
AScl Study [D# 5010-068
1.0 GEMERAL UQFDRMATION
Study Title Data Standard Sponsor
Sponsor's Representative
Sponsor's Lab Request# TestingFacility
PrincipalInvestigator ProjectDirector TestingFacilitDyirector DefinitivTeest Dates Data Validationand Report Review
Growth Inhibitioonf R1331 forGreen Alga (Selenatrum capticomutwn).
OECD Guideline201.
3M EnvironmentalTechnology and Safety Services,Building2-3E-09, 935 Bush Avenue, St.Paul,MN 55133; Tel No. (612)778-7452.
Susan Beach, 3M EnvironmentalTechnology and SafetyServices,Building2-3E-09, 935 Bush Avenue, St.Paul, MN 55133; Tel No. (612)778-7452.
R1331.
AScl CorporationA/ScI-Duluth Environmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811; Tel. No. (218) 722-4040.
Todd McGuire
Joe Amato
Donald Mount
March 25-29, 1997.
Alan Mozol
Report Signatu
Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport
Two yearsfrom the datestudy iscompleted.
ASCI Corporation/AScl-DuluthEnvironmental TestingDivision,4444 Airpark Boulevard,, Duluth,MN 55811; Tel.No. (218)722-4040.
Sponsor- 3M Company
Sponsor Study [D# R1331
2
ASci Corpomtion/ASel-Duluth Environmental TestingDivision
ASci Study [D# 5010-069
2.0OBJECTIVE(S)
To determintehe4-dmedianeffecctoncentrat(iEoCn50)andthe4-dnoobservabelfefect concentrat(iNoOnEC)ofthetesstubstancRe1,331,forgreenalga(Selenastmcmapricomutum) understattiecsctonditions.
This studywas conductedaccordingtoOECD Guideline201 (OECD 1993).
Test Substance
Test Organisms
AlgalMedium Exposure Chambers
3.0TEST METHODS
Properties:R1331, clearamber-coloredliquid,specificgravity=1.02 (water=l),stablea,nd completelysolublein water.
Testconcentrationv0:(algalmedium control)1,30,216,360,600, and 1000 mg/L. A stocksolutiownas preparedby mass additiontoalgal medium followedby mechanicalstirringE.ach testconcentratiownas made by volume additionof thestockto algalmedium.
Source: ATCC 22662
Inoculumpreparation:Algal stockwas subculturedin OECD recommended medium for4 days beforetestinitiation.
Use in test:Stockculturewas dilutedand spikedintotestsolutiontso obtainan initiaclelldensityof 1.0X 104 cellsper mi (1.3X 104 actual concentration). Preparation:Deionizedwaterwas spikedwithnutriensttocks(including: Na2EDTA) toOECD recommended concentrationsT.he most recent chemicalanalysisfordeionizedwaterisin Raw Data Package.
CharacteristicsA:t testtime,themedium had a pH of 8.00. Sterilizevdia 0.22 14m filtratiporniorto use in thetest. Type: Sterilizevdiaautoclave250-mi borosilicate-glEarsisenmeyerflasks sealedwith foam stoppers.
Use in test:100 mi of algalmedium or testsolution/chamber.Six control replicateasnd threereplicatepser testsubstanceconcentratiownere employed. During thetest,keptstoppered,exceptwhen experimental observationswere made.
Sponsor: 3M Company
Sponsor Study ID# R 1331
3
ASCJ Corporation/ASci-Duluth EnvironmentaTlestingDivision
ASel StudyID# 5010-OU
Incubation
Observations
Endpoint Calculations
Duration4:days.
DailyphotoperioCdo.ntinuoauts740ft-candluessingcool-white fluorescenltamps. Testchambers heldon shakertablesetat 100 rpm.
Temperature:25.0 2*C. Biological:Daily cellcountsusingdirectmicroscopicenumerationvia Improved Neubauer hemacytometer.
Water chemistry:(1)At testinitiatioand temination-pH, conductivity, and temperature(,2)daily-temperature.Alldeterminationwsere made accordingtoAPHA methods(1995). 1-,2-,3-,and 4-d EC50's,and 4-d NOEC based on both cellcountsand averagespecifigcrowth rates(,u).Statisticaanlalysesperformed using Trimmed Spearman-KarberMethod (EC50s) and lCp analysis.
4.0 RESULTS
Test SubstanceToxicity(Pertinendtataare in Tables1,2,and 3)
CellCount 1-d EC50: 233 mg/L (159-342). IA 1-dEC50: 160 mg/L (150-170).
CellCount 2-dECSO: < 130 mg/L. IA 2-dEC50: 251 mg/L (204-309).
CellCouni 3-d EC50: < 130 mg/L. IA 3-dECSO: 572 mg/L (434-756).
CellCount 4-dEC50: < 130 mg/L. IA 4-d ECSO: 664 mg/L (528-834).
CellCount 4-dNOEC (ICIO):23 mg/L (22-24). A 4-dNOEC (]CIO):96 mg/L (88-105).
Effectwas determinedto be algistatibcased on productionof r-ellesxposedduringthe definitivteestto the 1000 mg/L testsubstance concentration.
Sponsor: 3M Company
Sponsor Study ID# R1331
4
TestConditions
AScl Corpomtion/ASci-Duluth EnvironmentTaelstinDgivision
ASelStudyID# 5010-M
Temperatu(r*eC):24.8-25.8. Lightintensfitt-ycdls7:40. pH: 4.72-9.90. 1Specificconductivir(yAmhos@cm 129-186.--
5.0TEST QA/QC
QA/QC Criteria Algalcountsin controlmust increaseby at leasta factorof 16 duringthefirs3t-d of the
Testdurationmust be 4-d.
Data
During thefirs3t-d of thetestcontrolalgal countsincreasedby a factorof approximately 157.
The testdurationwas 4-d.
6.0 REFERENCES
American PublicHealthAssociatio(nAPHA). 1995.StandardMethods fortheExaminationof Water and Wastewater.APHA, Washington,D.C.
OrganizationforEconomic Cooperationand Development (OECD). 1993.OECD Guidelinesfor Testingof Chemicals.OECD PublicatioInnformatioCnenter,Washington,D.C.
Hamilton,M.A., R.C. Russo,and R.V. Thurston,1977, Trimmed Spearman-KarberMethod forEstimatingMedian LethalConcentrationisnToxicityBioassays.Environ.Sci.Technol. 11(7):714-719;Correctio1n2 (4):417 (1978).
US EPA. 1993.A LinearInterpolatiMoenthod forSublethaTloxicity:The Inhibition Concentratio(nlCp)Approach (Version2.0).EnvironmentalResearchLaboratory,Duluth, Minnesota.
Sponsor: 3M Company
Sponsor Study ID# R1331
5
AScl Corporation/ASci-Duluth Environmental TestingDivision
ASci Study ID# 5010-068
TABLE 1. ECso'S, 95% ConridenceIntervalsa,nd 4-d NOEC'S from AlgalGrowth Inhibition Test with R1331
cell count
Data2
1-d EC50 2-d EC50 3-d EC50 4-d ECSO 4-d NOEC (1clo)
(nigIL) 233 < 130 < 130 < 130 23
95 % Confidence Interval(mg/L)
159-342 Not Calculable Not Calculable Not Calculable
22-24
:Endpoint
Average Specific Growth Rate (A)
DaW Time 1-d EC50 2-d ECSO 3-d EC50 4-d ECSO 4-d NOEC (1clo)
(mg/L) 160 251 572 664 96
95 % Confidence Interval(mg[L)
150-170 204-309 434-756 528-834 88-105
Values Based on Nominal Test Concentrations
Sponsor: 3M Company
Sponsor Study ID# R1331
6
AScl Corporation/AScl-Duluth Environmcntal TcstingDivision
AScl Study ID# 5010-068
TABLE 2. Cellsper ml and PercentageDifferencefrom Mean ControlValuefor Selenastrum capticornulumExposed to R1331
Control 130 1
360 600 1000
A
B
c
D E
F
Mean
A
B
c Mean
A B c
Mean
A
B c
Mean
A B c
Mean
A B c Mean
Id
4
2
3
s
3 5 3.7
2 4
2
2.7(-28)
2 1 1
1.3(-64)
3
1 2
2.0(-46)
1 1 2 1.3(-64)
2 1 2 1.7(-55)
Cells/n-d(104)
2d
3d
35
200
35
215
3S
200
40
195
31
205
30
210
34.3
204
13 17 14 14.7(-57)
69 61 64 64.7 (-68)
5 6
5
5.3 (-84)
39 49 40 42.7 (-79)
4 6 5
5.0(-85)
3 4 5 4.0 (-88)
4 4 2 3.3 (-90)
23 25 32
26.7(-87)
18 14 14 15.3 (-92)
10 6 10 8.7 (-96)
4d
726 660 627 616 704 638 662
310 270 27S 285 (-57)
195
171 165 177(-73)
75 78 93
82.0(-88)
41 39 33 37.7 (-94)
12 13 14 13.0(-98)
a ParenthetiVcalues % Differencefrom ControlMean Value 0-d Mean AlgalCount 1.3X 104
Sponsor: 3M Company
SponsorStudyID# R1331
7
AScl Corporation/ASci-Duluth EnvironmentalTestingDivision
ASel Study ID# 5010-068
TABLE 3. Average SpecificGrowth Rates (a)and PercentageDifferencefrom Mean Control Value for Selexastramcapricomatum Exposed to R1331
::C%.@.., control
Id 1.046
Average SpecibcGrowth Rate (itr
2d
3d
1.636
1.686
4d 1.558
130
0.731(-30)
1.213(-26)
1.302(-23)
1.348(-13)
216
0(-100)
0.703(-57)
1.164(-31)
1.228(-21)
360
0.431(-59)
0.674(-59)
1.007(-40)
1.036(-34)
600
0(-100)
0.562(-66)
0.822(-51)
0.942(-46);
1000
0.268(-74)
0.466(-72)
0.634(-62)
0.576(-63)
In C*Hs perml In C4;lls/mtlo ta- to
b ParentheticValues % Differencefrom ControlMean Value
0-d Mean AlgalCount 1.3 X 104
Sponsor: 3M Company Sponsor Study ID# R1331