Document dnB77DwpnNb08QBJd585QGK25

C O V /H H > THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M St. Paul, Minnesota FINAL REPORT Study Title: 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Author: Peter J. Thomford, PhD Study Completion Date: December 13, 2001 Testing Facility: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Laboratory Study Identification: Covance 6329-212 Sponsor Study Identification: 3M T-6316.1 Volume I of XII Page 1 of4873 Covance 6329-212 3M T-6316.1 COMPLIANCE STATEMENT 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats All aspects of this study were in accordance with the Food and Drug Administration Good Laboratory Practice Regulations as set forth in Title 21 of the Unites States Code of Federal Regulations, Part 58, issued December 22, 1978 (effective June 20, 1979), and with any applicable amendments. 2 Covance 6329-212 3M T-6316.1 QUALITY ASSURANCE STATEMENT This report has been reviewed by the Quality Assurance Unit of Covance Laboratories Inc., in accordance with the Food and Drug Administration (FDA) Good Laboratory Practice Regulations, 21 CFR 58. The following inspections were conducted and findings reported to the study director and study director management. Inspection D ates From To 22 Jan 98 22 Jan 98 02 M ar 98 02 M ar 98 28 A pr 98 28 A pr 98 11 M a y 98 11 M a y 98 27 O ct 98 28 O ct 98 28 Dec 98 28 Dec 98 12 M ar 99 12 M ar 99 26 A pr 99 26 A pr 99 02 Jun 99 04 Jun 99 22 Jun 99 02 Jul 99 26 Jul 99 26 Jul 99 20 Jan 00 20 Jan 00 25 Jan 00 25 Jan 00 11 M a y 00 16 M a y 00 10 A p r 00 26 M ay 00 25 M ay 00 30 M ay 00 16 M ay 00 22 M ay 00 06 Jun 00 07 Jun 00 13 Ju n 00 14 Ju l 00 20 A p r 01 27 A p r 01 31 O ct 01 31 O ct 01 24 O ct 01 02 N o v 01 06 N o v 01 06 N o v 01 06 N o v 01 06 N o v 01 06 N o v 01 06 N o v 01 13 N o v 01 13 N o v 01 Phase Protocol R eview T est A rticle A dm inistration A nalytical L aboratory Inspection Food C onsum ption A nalytical L aboratory Inspection B ody W eights T est A rticle Preparation A nim al O bservations Protocol A m endm ent R eview D ata R eview T est A rticle A dm inistration Protocol A m endm ent R eview P o stlife D ata R eview D ata R eview Protocol A m endm ent R eview D ata R eview D ata R eview R eport R eview R eport R eview Protocol A m endm ent R eview R eport R eview Protocol A m endm ent R eview Protocol A m endm ent R eview Protocol A m endm ent R eview Protocol A m endm ent R eview D ate R eported to Study D irector and Study D irector M anagem ent 22 Jan 98 02 M ar 98 28 A pr 98 11 M a y 98 28 O ct 98 28 Dec 98 12 M ar 99 26 A pr 99 04 Jun 99 02 Jul 99 26 Jul 99 20 Jan 00 25 Jan 00 19 M ay 00 26 M ay 00 30 M ay 00 01 Jun 00 07 Jun 00 14 Jul 00 30 A p r 01 31 O ct 01 02 N o v 01 06 N o v 01 06 N o v 01 06 N o v 01 13 N o v 01 3 Covance 6329-212 3M T-6316.1 STUDY IDENTIFICATION 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Test Material: Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol Sponsor: 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000 Study Monitor: Andrew M. Seacat, PhD 3M 651.575.3161 Alternate Study Monitor: John Butenhoff, PhD, DABT 3M 651.733.1962 Study Location: Covance Laboratories Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704-2595 Study Director: Peter J. Thomford, PhD Covance Laboratories Inc. PO Box 7545 Madison, Wisconsin 53707-7545 608.241.7207 Study Timetable: Study Initiation Date: Inlife Start Date: Inlife End Date: Study Completion Date: January 21, 1998 January 26, 1998 January 31, 2000 December 13, 2001 4 Covance 6329-212 3M T-6316.1 KEY PERSONNEL 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Study Director: Study Toxicologist: Study Coordinator: Supervisor, Small-Animal Toxicology: Supervisor, Dose Formulation: Supervisor, Chemistry/Tox Support: Associate Director, Laboratory Animal Medicine: Clinical Pathologist: Supervisor, Clinical Pathology: Anatomic Pathologist: Supervisor, Anatomic Pathology: Supervisor, Anatomic Pathology: Senior Staff Scientist: Biostatistician: Principal Investigator, Cell Proliferation: Principal Investigator, Electron Microscopy: Peter J. Thomford, PhD Thomas E. Ryan, BS Cindy L. Powers, BS Jeni Barman, AAS, ALAT, LAT Dixie Bushee, BS, LATG Mark Hoffmann, BS, MBA Donna J. Clemons, DVM Diplomate, ACLAM Robert L. Hall, DVM, PhD Diplomate, ACVP (Clinical Pathology) Ronald Markevitch, BS, MT (ASCP) James L. Carter, DVM, PhD Diplomate, ACVP Kimberly W. Durland, BS, HT Laurie J. Schuller, BA, LAT Cynthia Y.C. Liu, MA Ajit K. Thakur, PhD Carolyn Moyer, DVM Diplomate, ACVP Pathology Associates International James B. Nold, DVM, PhD Diplomate, ACVP Pathology Associates International 5 Covance 6329-212 3M T-6316.1 VOLUME I OF XII CONTENTS Page ABSTRACT.......................................................................................................................11 PURPOSE.......................................................................................................................... 17 REGULATORY COMPLIANCE..................................................................................... 17 TEST MATERIAL, VEHICLE, AND CARRIER........................................................... 17 Test Material..................................................................................................................17 Vehicle........................................................................................................................... 17 Carrier............................................................................................................................ 18 Reserve (Archive) Samples............................................................................................18 Disposition.....................................................................................................................18 TEST ANIMALS AND HUSBANDRY........................................................................... 18 Animals.......................................................................................................................... 18 Identification..................................................................................................................18 Justification....................................................................................................................19 Husbandry......................................................................................................................19 Acclimation....................................................................................................................19 PROCEDURES................................................................................................................. 20 Group Designations and Dose Levels...........................................................................20 Dosing Procedures.........................................................................................................21 Dose Analysis................................................................................................................ 23 Observation of Animals................................................................................................ 23 Clinical Pathology.........................................................................................................24 Serum Analyses............................................................................................................. 25 Anatomic Pathology......................................................................................................26 Proliferation Cell Nuclear Antigen Evaluation............................................................. 27 Palmitoyl-CoA Oxidase Tissue Collection and Analyses............................................ 28 Statistical Evaluation.....................................................................................................30 Survival and Tumor Analysis....................................................................................... 30 Record Retention........................................................................................................... 32 RESULTS AND DISCUSSION....................................................................................... 33 Dose Analysis................................................................................................................ 33 Observation of Animals.................................................................................................34 Clinical Pathology.........................................................................................................35 Anatomic Pathology......................................................................................................36 Proliferation Cell Nuclear Antigen Results.................................................................. 37 Electron Microscopy Results........................................................................................ 38 CONCLUSION................................................................................................................. 38 6 Covance 6329-212 3M T-6316.1 VOLUME I OF XII CONTENTS (Continued) Page SIGNATURES.................................................................................................................. 39 REFERENCES.................................................................................................................. 40 PATHOLOGY REPORT...................................................................................................41 PATHOLOGY PEER REVIEW REPORT.......................................................................56 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC AND NEOPLASTIC LESIONS............................................................................................. 61 COMMENTS ON THE DATA........................................................................................ 84 CODES, ABBREVIATIONS, AND UNITS.....................................................................86 General Codes and Abbreviations................................................................................. 87 Codes for Clinical Pathology........................................................................................ 88 Abbreviations and Units for Clinical Hematology........................................................ 91 Abbreviations and Units for Clinical Chemistry........................................................... 92 Abbreviations and Units for Clinical Urinalysis........................................................... 93 Abbreviations and Units for Urine Chemistry.............................................................. 94 Codes for Anatomic Pathology..................................................................................... 95 FIGURES 1 Adjusted Survival Data (%) - Males........................................................................97 2 Adjusted Survival Data (%) - Females................................................................... 98 3 Mean Body Weight Data (g) - Males......................................................................99 4 Mean Body Weight Data (g) - Females.................................................................100 5 Mean Food Consumption Data (g) - M ales.......................................................... 101 6 Mean Food Consumption Data (g) - Females....................................................... 102 TABLES 1 Results of Homogeneity Analyses (ppm)..............................................................103 2 Results of Stability Analyses (ppm)...................................................................... 111 3 Results of Dose Preparation Analyses (ppm)........................................................ 115 4 Survival Data......................................................................................................... 124 5 Summary of Clinical Observations........................................................................ 142 6 Summary of Mass Observations............................................................................ 160 7 Summary of Body Weight Data (g)....................................................................... 186 8 Summary of Body Weight Change Data (g)......................................................... 198 9 Summary of Food Consumption Data (g)............................................................. 200 10 Summary of Test Material Consumption Data (mg/kg/day)................................. 212 11 Summary of Clinical Hematology Data - Week 8.................................................224 12 Summary of Clinical Hematology Data - Week 14...............................................228 7 Covance 6329-212 3M T-6316.1 VOLUME I OF XII CONTENTS (Continued) Page TABLES 13 Summary of Clinical Hematology Data - Week 27...............................................232 14 Summary of Clinical Hematology Data - Week 53...............................................236 15 Summary of Clinical Chemistry Data - Week 4 ...................................................240 16 Summary of Clinical Chemistry Data - Week 8 ...................................................242 17 Summary of Clinical Chemistry Data - Week 14..................................................248 18 Summary of Clinical Chemistry Data - Week 27..................................................252 19 Summary of Clinical Chemistry Data - Week 53..................................................256 20 Summary of Clinical Chemistry Data - Week 105................................................260 21 Summary of Clinical Urinalysis Data - Week 8....................................................262 22 Summary of Clinical Urinalysis Data - Week 14..................................................264 23 Summary of Clinical Urinalysis Data - Week 27..................................................266 24 Summary of Clinical Urinalysis Data - Week 53..................................................268 25 Summary of Urine Chemistry Data - Week 8 ....................................................... 270 26 Summary of Urine Chemistry Data - Week 14.....................................................272 27 Summary of Urine Chemistry Data - Week 27.....................................................274 28 Summary of Urine Chemistry Data - Week 53.....................................................276 29 Summary of Organ Weight Data - Week 4 Sacrifice........................................... 278 30 Summary of Organ Weight Data - Week 8 Sacrifice........................................... 280 31 Summary of Organ Weight Data - Week 14 Sacrifice......................................... 295 32 Summary of Organ Weight Data - Week 53 Sacrifice......................................... 325 33 Incidence of Macroscopic Observations - Week 4 Sacrifice.................................340 34 Incidence of Macroscopic Observations - Week 8 Sacrifice.................................341 35 Incidence of Macroscopic Observations - Week 14 Sacrifice.............................. 353 36 Incidence of Macroscopic Observations - Week 53 Sacrifice.............................. 365 37 Incidence of Macroscopic Observations - Terminal and Recovery Sacrifices......378 38 Incidence of Macroscopic Observations - Unscheduled Deaths...........................397 39 Incidence of Macroscopic Observations - All Animals........................................ 433 VOLUME II OF XII TABLES 40 Incidence of Microscopic Observations - Week 8 Sacrifice................................ 474 41 Incidence of Microscopic Observations - Week 14 Sacrifice............................... 486 42 Incidence of Microscopic Observations - Week 53 Sacrifice............................... 499 43 Incidence of Microscopic Observations - Terminal and Recovery Sacrifices...... 514 44 Incidence of Microscopic Observations - Unscheduled Deaths............................ 537 45 Incidence of Microscopic Observations - All Animals........................................ 583 8 Covance 6329-212 3M T-6316.1 VOLUME II OF XII CONTENTS (Continued) Page TABLES 46 Incidence of Severity of Selected Microscopic Observations - Week 8 Sacrifice.............................................................................................................. 635 47 Incidence of Severity of Selected Microscopic Observations - Week 14 Sacrifice.............................................................................................................. 641 48 Incidence of Severity of Selected Microscopic Observations - Week 53 Sacrifice.............................................................................................................. 647 49 Incidence of Severity of Selected Microscopic Observations - Terminal and Recovery Sacrifice..............................................................................................653 50 Incidence of Severity of Selected Microscopic Observations - Unscheduled Deaths................................................................................................................ 661 51 Incidence of Severity of Selected Microscopic Observations - All Animals....... 668 APPENDIX 1.................................................................................................................. 677 Protocol Deviations.....................................................................................................678 VOLUME III OF XII APPENDIX 2 .................................................................................................................. 685 Individual Animal Fate Data....................................................................................... 686 Individual Clinical Observations................................................................................. 711 VOLUME IV OF XII APPENDIX 3................................................................................................................ 1226 Individual Body Weight Data (g).............................................................................. 1227 APPENDIX 4 ................................................................................................................ 1306 Individual Food Consumption Data (g).....................................................................1307 Individual Test Material Consumption Data (mg/kg/day)........................................ 1384 APPENDIX 5................................................................................................................ 1456 Individual Clinical Hematology Data........................................................................1457 Individual Clinical Chemistry Data...........................................................................1499 Individual Clinical Urinalysis Data...........................................................................1541 Individual Urine Chemistry Data.............................................................................. 1583 9 Covance 6329-212 3M T-6316.1 VOLUME V OF XII CONTENTS (Continued) Page APPENDIX 6................................................................................................................ 1599 Individual Anatomic Pathology Data........................................................................1600 VOLUME VI OF XII APPENDIX 6 (Continued)........................................................................................... 2094 Individual Anatomic Pathology Data....................................................................... 2095 VOLUME VII OF XII APPENDIX 6 (Continued)........................................................................................... 2621 Individual Anatomic Pathology Data....................................................................... 2622 VOLUME VIII OF XII APPENDIX 6 (Continued)........................................................................................... 2958 Individual Anatomic Pathology Data....................................................................... 2959 APPENDIX 7................................................................................................................ 3299 Cell Proliferation Report.......................................................................................... 3300 APPENDIX 8................................................................................................................ 3338 Electron Microscopy Report.................................................................................... 3339 VOLUME IX OF XII APPENDIX 9................................................................................................................ 3380 Covance 6329-228.................................................................................................... 3381 10 ABSTRACT Covance 6329-212 3M T-6316.1 The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE), when administered in the diet to rats for at least 104 weeks. Male and female Crl:CD(SD)IGS BR rats were assigned to seven groups (70 animals/sex/group for Groups 1, 4, and 5; 60 animals/sex/group for Groups 2 and 3, and 40 animals/sex/group for Groups 6 and 7). Each group received diets containing the vehicle, acetone, (Group 1), or 3 (Group 2), 30 (Group 3), 100 (Groups 4 and 6), or 300 (Groups 5 and 7) ppm N-EtFOSE. Group 6 was placed on recovery after 52 weeks of treatment. Five animals/sex/group in Groups 1 through 5 were sacrificed during Week 4 and livers were collected. Five animals/sex/group in Groups 1 and 5 were sacrificed during Week 8. Five animal/sex/group in Groups 1 through 4 were sacrificed during Week 14. During Week 52, five animals/sex/group in Group 1 and 10 animals/sex in Group 4 were necropsied. The remaining animals in Groups 1 through 4 were sacrificed at the terminal sacrifice during Week 105. The remaining animals in Group 6 were sacrificed during Week 105 after 52 weeks of recovery. Animals that died on test or were sacrificed at unscheduled intervals were also necropsied, but organ weights were not recorded. After 7 weeks of treatment at 300 ppm, three males were found dead or sacrificed in a moribund condition. There was a 20% or greater difference in body weights for males and females given 300 ppm compared to the control values. It was decided that 300 ppm exceeded the maximum tolerated dose and Groups 5 and 7 were terminated during Week 8. An ancillary study was conducted under Covance 6329-228. Seventy animals/sex/group were assigned to two groups, Groups 8 and 9. Animals received diets containing the vehicle, acetone, or 1 ppm N-EtFOSE, respectively. The complete report is presented in Appendix 9, Volume VIII. Clinical observation, body weight, and food consumption data were recorded. Blood and urine samples were collected for hematology, coagulation, clinical chemistry, urinalysis, and urine chemistry tests at specified time points. Selected organs were weighed at the interim sacrifices during Weeks 8, 14, and 53. Adrenals, brain, eyes, kidneys, liver, 11 Covance 6329-212 3M T-6316.1 mesenteric lymph nodes, pancreas, spleen, testes, and ovaries from the animals necropsied at the Week 8 and 14 interim sacrifices were examined microscopically. Microscopic examinations were done on tissues from each animal in Groups 1 and 4 sacrificed at the Week 53 interim sacrifice and terminal sacrifice, and any animals that died on test or were sacrificed at unscheduled intervals. Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, mammary glands (females) and urinary bladder from each animal in Groups 2, 3, and 6 sacrificed at the terminal or recovery sacrifices were examined microscopically Additional liver samples were also collected for evaluation of palmitoyl-CoA oxidase activity and proliferation cell nuclear antigen from five animals/sex/group in Groups 1 through 5 during Week 4, five animals/sex/group in Groups 1 and 5 during Week 8, and five animals/sex/group in Groups 1 through 4 during Week 14. Liver sections from five animals/sex/group from Groups 1 and 4 were stained with Oil Red "O" and examined microscopically. In addition, liver samples from five animals/sex/group were processed and evaluated by electron microscopy. Thirty-six control males and 37 control females, 28 males and 37 females given 3 ppm, 28 males and 35 females given 30 ppm, 33 males and 30 females given 100 ppm (Group 4) for 104 weeks, and 30 males and 28 females given 100 ppm for 52 weeks (Group 6) died on test or were sacrificed in a moribund condition. There were no test material-related effects on mortality. The most frequent cause of death was pituitary adenomas. Clinical observations (thin and hunched appearance, hypoactivity, red discharge from the nose and eyes, few/no/liquid feces, ataxia, squinted eyes, recumbent behavior, and rough/brown/red hair coat) were similar in nature and in the total number of affected animals in all dose groups. There was a dose-related effect on body weights for males and females given 30 or 100 ppm. After 52 weeks of treatment, mean body weights for males given 3, 30, or 100 ppm were 102, 94, or 88% of control values, respectively. Mean body weights for females given 3, 30, or 100 ppm were 94, 89, or 74% of control values, respectively. After 104 weeks of treatment, mean body weights for males given 3, 30, or 100 ppm were 98, 96, and 87% of control values, respectively. Mean body weights for females 12 Covance 6329-212 3M T-6316.1 given 3, 30, or 100 ppm were 107, 109, and 82% of control values, respectively. The body weight changes seen at 100 ppm were considered of toxicological significance. Mean cumulative body weight change was statistically significantly lower for males given 30 or 100 ppm diets for Weeks 1 through 15 and 15 through 29. Mean body weight change was statistically significantly lower for females given 30 or 100 ppm for Weeks 1 through 15 and for Weeks 29 to 57 and for females given 100 ppm for Weeks 15 through 29. There was an increase in the rate of body weight gain for Group 6 animals after they were placed on recovery after 52 weeks of treatment. Mean food consumption for males and females fed the 100 or 300-ppm diets as well as the males fed the 30-ppm diet was generally lower compared with that of the control group, and these values were frequently statistically significantly different from control values. Mean food consumption for males fed the 3-ppm diets and females fed the 3- or 30-ppm diets were comparable to controls with only sporadic differences in mean food consumption noted. The changes seen in food consumption at 100 ppm were considered toxicologically significant and in general correlated with the changes in body weight. The amount of test material consumed by the animals on a mg/kg of body weight/day basis was as follows. Group 2 3 4 5/7 Target Dose Levels (ppm) 3 30 100 300 Achieved Dose Levels - Range (mg/kg body weight/day) Males Females 0.259 -0.081 0.109 -0.307 0.860 -2.618 1.029 -3.070 3.065 - 8.724 4.213 - 10.166 15.803-25.408 19.568 -29.270 Dietary administration of N-EtFOSE for approximately 52 weeks was associated with mildly decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; mildly lower glucose and mildly higher urea nitrogen for males and females given 100 ppm (predominantly at Weeks 14 and 27); moderately higher albumin and moderately lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and moderately to markedly lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. The effect on cholesterol was reversed following the 52-week recovery. Administration of N-EtFOSE at 3 ppm for approximately 52 weeks had no effects on clinical pathology test results. Administration of N-EtFOSE for approximately 104 weeks was associated with moderately lower cholesterol for males 13 Covance 6329-212 3M T-6316.1 given 30 or 100 ppm and moderately lower triglycerides for males and females given 100 ppm. At a dose level of 300 ppm for approximately 8 weeks, administration ofN-EtFOSE was associated with many effects on clinical pathology results including lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase (also observed at Week 4). Most of the effects were moderate to marked in magnitude. The percentage of proliferating hepatocytes (PI) was significantly increased only in males exposed to 300 ppm ofN-EtFOSE at 4 weeks on study. This increase was not observed after 8 weeks on study at which time the 300 ppm dose group was removed from the study. The NOEL for hepatocellular proliferation in males was the 100 ppm dose group. Cell proliferation was not statistically significant in females at 4, 8, or 14 weeks on study in any dose group. At the interim sacrifices, body weights were lower and absolute and relative liver weights were higher for animals given 100 or 300 ppm of the test material. Macroscopically, diffusely dark livers were only observed for animals given 100 ppm of the test material. Enlargement, mottling, and masses of the liver were slightly more frequent for animals given 100 ppm than for the controls. Administration of the test material caused an increase in cystic degeneration, hepatocellular hypertrophy, hepatocellular vacuolation, necrosis and hemorrhage of the liver. At the Week 8 sacrifice, hepatocellular hypertrophy, necrosis and hemorrhage were observed for animals given 300 ppm. Hepatocellular hypertrophy was observed only at the Week 14 sacrifice for animals given 30 or 100 ppm, and hepatocellular vacuolation and hypertrophy were present at the Week 53 sacrifice only for animals given 100 ppm. The hepatic changes were considered toxicologically important. Test material related hepatic findings for animals given 100 ppm at the terminal sacrifice were cystic degeneration (males only) and hepatocellular hypertrophy and vacuolation. 14 Covance 6329-212 3M T-6316.1 The incidence of hepatocellular adenomas was slightly higher for males and females given 100 ppm. The higher incidence was statistically significant for females. The only hepatocellular carcinoma in the study was present for a terminal sacrifice female given 100 ppm. Liver samples were obtained from each of 20 animals (10/sex/group from the control and mid-high dose groups) during necropsy at terminal sacrifice for examination by transmission electron microscopy (TEM). Liver samples were assessed for changes that may likely occur due to chronic toxicity of the test material. Electron microscopic evaluation identified mild to moderate changes related to treatment. Mild to moderate smooth endoplasmic reticulum hyperplasia and minimal to mild hepatocellular hypertrophy were the prominent features found to be different between the mid-high dose group and control animals. Other changes included a slight increase in the amount of lipid accumulation and minor increases in lipofuscin were found in the treated animals compared with controls. Male and female Crl:CD(SD)IGS BR rats were administered N-Ethyl Perfluorooctanesulfonamido Ethanol at doses of 0, 3, 30 and 100 ppm at least 104 weeks. There were no significant effects on survival. In males, combined thyroid follicular cell adenoma/carcinoma was significantly increased as compared to controls at 100 ppm (p = 0.011) which was due primarily to an increase in follicular cell adenoma (p = 0.011). Thyroid follicular cell carcinoma alone was not increased. The relationship of this finding to treatment is questionable for several reasons: thyroid follicular cell tumors in historical controls from this laboratory are relatively common (occur with an incidence of greater than 1%), however, no thyroid follicular cell tumors occurred in male controls from this study, there was no evidence on nonneoplastic lesions of the thyroid, the increase at 100 ppm was within the range of historical controls, and a clear dose-response relationship was not present. In females, the incidence of combined hepatocellular adenoma/carcinoma was significantly increased in the 100 ppm dose group as compared to controls (p = 0.007) as a result of a significant increase in hepatocellular adenoma in this group (p = 0.012). There was no increase for trend or pairwise for hepatocellular carcinoma alone. The only hepatocellular carcinoma observed in this study was in a 100-ppm, terminal-sacrifice female. Recovery females in the 100 ppm dose group experienced a marginally significant increase in hepatocellular adenoma (p = 0.05). It is noteworthy that, in females, liver effects were only observed at 100 ppm and included the finding of a 15 Covance 6329-212 3M T-6316.1 hepatocellular adenoma at the Week 53 sacrifice. Significant negative trends and pairwise decreases at 100 ppm occurred among females for mammary tumors (fibroadenoma, combined fibroadenoma/adenoma, and combined fibroadenoma/adenoma/carcinoma). Trends for pituitary adenoma and combined adenoma/carcinoma were also negative in females. 16 PURPOSE Covance 6329-212 3M T-6316.1 The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE), when administered in the diet to rats for at least 104 weeks. REGULATORY COMPLIANCE All aspects of this study were conducted in compliance with the Food and Drug Administration Good Laboratory Practice Regulations as set forth in Title 21 of the United States Code of Federal Regulations, Part 58, issued December 22, 1978 (effective June 20, 1979), and with any applicable amendments. The protocol was reviewed and approved by the Institutional Animal Care and Use Committee at Covance. TEST MATERIAL, VEHICLE, AND CARRIER Test Material The test material, T-6316 (narrow range N-ethyl perfluorooctanesulfonamido ethanol, N-EtFOSE), a blend of Lot Nos. 30035, 30037, and 30039, is a waxy solid and is 98.1% N-EtFOSE (w/w) (see Protocol Deviations for exceptions). It was received at Covance on June 20 and July 21, 1997. To avoid repeated heating and sublimation, the two shipments of test material were heated, combined, and predetermined quantities of test material were aliquoted into polyethylene (LDPE) containers. The test material was stored at room temperature. Information on synthesis methods, stability, composition, or other characteristics that define the test material was provided by the Sponsor and is on file at with the Covance. Vehicle The vehicle was acetone (Spectrum Chemical Mfg. Corp., New Brunswick, New Jersey and Gardena, California), Lot Nos. LW0044 (99.96% pure), LH0253 (99.8% pure), and 17 Covance 6329-212 3M T-6316.1 NS0231 (99.8% pure). The shipments were received at Covance on April 9 and June 23, 1997, and September 8, 1998, respectively. The vehicle was stored at room temperature. Information on synthesis methods, composition, or other characteristics that define the acetone is on file with the manufacturer. Carrier Certified rodent diet (#5002 meal, LabDiet by Purina Mills, Inc.) was used as supplied. The lot numbers are recorded in the data. The diet is routinely analyzed by the manufacturer for nutritional components and environmental contaminants. Reserve (Archive) Samples A reserve sample (5 g) of the test material was taken and stored at room temperature. This sample was transferred to the Sponsor after completion of the inlife phase. Disposition The remaining test material was returned to the Sponsor on August 8, 2000. TEST ANIMALS AND HUSBANDRY Animals Male and female Crl:CD(SD)IGS BR rats were obtained from the Raleigh, North Carolina, facility of Charles River Laboratories on January 6, 1998. At initiation of treatment, the animals were 45 to 51 days old; the males weighed from 202 to 310 g (see Protocol Deviations for exceptions), and the females weighed from 133 to 194 g. Identification Each animal was assigned a temporary number upon arrival. Before initiation of treatment, a microchip identification device was implanted into each animal. After randomization for placement on test, each animal was assigned a permanent number, and 18 Covance 6329-212 3M T-6316.1 the microchip was coded with the permanent number. All data for an animal are recorded under these numbers. Justification Historically, rats have been used in safety evaluation studies and are recommended by appropriate regulatory agencies. Husbandry Animal Room 3045 was used for this study. Environmental controls for the animal room were set to maintain 18 to 26oC, a relative humidity of 30 to 70%, a 12-hour light/12-hour dark cycle, and a minimum of 10 room air changes/hour. The animals were housed individually (except for the first 6 days of acclimation when animals were group-housed) in stainless-steel cages. Some animals were placed in polycarbonate cages during the study when health problems dictated. Certified rodent diet (#5002 meal, LabDiet by Purina Mills, Inc.) was provided a d libitum during acclimation. Water was provided a d libitum (see Protocol Deviations for exceptions). Samples of the water are analyzed for specified microorganisms and environmental contaminants. The results are on file with Covance-Madison. There were no known contaminants in the diet or water at levels that would have interfered with this study. Animals not selected for the study (45 males and 41 females) were used for training procedures. Two females were found dead during acclimation. Acclimation Four hundred fifty-five males and 453 females were received on January 6, 1998, and acclimated in Animal Room 3045 for 20 days before initiation of treatment. In general, animals in this shipment appeared healthy. During acclimation, the animals were 19 Covance 6329-212 3M T-6316.1 examined for abnormalities indicative of health problems and body weights were recorded for all animals at randomization. PROCEDURES This study was conducted in accordance with the Protocol dated January 21, 1998, and Protocol Amendment Nos. 1 through 8. Protocol deviations are in Appendix 1. An ancillary study, Covance 6329-228, was conducted in accordance with its protocol, April 1, 1998 and Protocol Amendment Nos. 1through 5. The complete report is presented in Appendix 9. Group Designations and Dose Levels The animals used for this study were examined by a laboratory animal veterinarian on January 9, 1998, and found to be suitable for study consideration. Selection of animals for the study was based on acclimation data. Animals were assigned to treatment groups using a computerized blocking procedure designed to achieve body weight balance with respect to treatment group. At the time of randomization, the weight variation of the animals did not exceed +2 standard deviations of the mean body weight for each sex. Group mean body weights were analyzed using Levene's test for homogeneity of variance at the 5.0% probability level and found to be homogeneous. Animals were assigned to the study according to the following design. 20 Covance 6329-212 3M T-6316.1 No. of Animals Dietary Levels Group Male Female (ppm N-EtFOSE)a 1 (Control)b,c,d,e 70 70 0 2 (Low)c 60 60 3 3 (Mid)c 60 60 30 4 (Mid-High)c,e 70 70 100 5 (High)c,d 70 70 300 6 (Mid-High Recovery/ 40 40 100 7 (High Recovery/ 40 40 300 a T-6316 is 98.1% n-ethyl perfluorooctanesulfonamido ethanol N-EtFOSE); dose levels are expressed as ppm of N-EtFOSE. b The control animals received the basal diet/acetone only. c Five animals/sex/group in Groups 1through 5 were sacrificed during Week 4, and five animals/sex/group in Groups 1 through 4 were sacrificed during Week 14 for hepatocellular proliferation rate measurements and biochemical analyses (palmitoyl-CoA oxidation). d Five animals/sex/group in Groups 1 and 5 were sacrificed during Week 8; the remaining animals in Groups 5 and 7 were sacrificed and discarded during Week 8. e Five animals/sex in Groupl and 10 animals/sex in Group 4 were designated as interim sacrifice animals and were sacrificed after at least 52 weeks of treatment. f Animals in Group 6 were treated for at least 52 weeks, then treatment was discontinued, and the animals were observed for reversibility, persistence, or delayed occurrence of toxic effects for at least 52 weeks posttreatment. During recovery, the animals received basal diet only. Dosing Procedures Dose Preparation. Dietary concentrations were based on the test material as supplied. Diets were prepared at least once every 4 weeks. Preparation of Diet for Group 1. The appropriate amount of certified diet was weighed and mixed with an appropriate volume of acetone. Preparation of Stock. A stock preparation was made using the required amount of test material dissolved in the required volume of acetone. Diets for Groups 4/6 and 5/7 (Base Diet). For the 300-ppm base diet, a premix consisting of a portion of the certified diet with the required amount of the stock preparation was mixed using a Hobart mixer. For the 100-ppm diet, a premix consisting of a portion of the certified diet, the required amount of the stock preparation, 21 Covance 6329-212 3M T-6316.1 and additional acetone was mixed using a Hobart mixer. These premixes were transferred to a mixer with additional certified diet and mixed thoroughly. Diet for Group 2. For the 3-ppm diet, a premix consisting of a portion of the certified diet, the required amount of the prepared 300-ppm diet, and the required volume of acetone, was mixed and then transferred to a mixer with additional certified diet and mixed thoroughly. Diet for Group 3. For the 30-ppm diet, the required amount of the prepared 300-ppm diet and the required volume of acetone were mixed thoroughly with the appropriate amount of certified feed. Samples for dose analyses were taken directly from the mixing bowl. The diets were stored at room temperature (see Protocol Deviations for exceptions) in covered containers until dispensed into feeding jars. Method of Administration. Dietary admixture was used because the potential route of exposure in humans is oral. The dose preparations were administered a d libitum (see Protocol Deviations for exceptions). Animals in Groups 1 through 4 received the test diet for at least 104 weeks (up to Day 732, Week 105, as applicable). Animals in Group 6 received the test diet for 52 weeks. After 7 weeks of treatment at 300 ppm, three males were found dead or were sacrificed in a moribund condition. There was a 20% or greater difference in body weights for males and females given 300 ppm compared to control values. It was decided that 300 ppm exceeded the maximum-tolerated dose and Groups 5 and 7 were terminated during Week 8. Retention Samples. Samples (approximately 100 g) were taken from each dose preparation and stored at room temperature (see Protocol Deviations for exceptions). Unless used for analyses, retention samples from the treated groups were discarded at least 1 month after completion of the inlife phase (see Protocol Deviations for exceptions). Samples from the control diets prepared for Weeks 1 through 24 were shipped to the Sponsor. 22 Covance 6329-212 3M T-6316.1 Dose Analysis Analyses for the concentration of test material in the dose preparations were done by Covance using an analytical method, MP-M312-MA, validated by Covance. Homogeneity was determined for all concentrations once pretest and for preparations for Weeks 1 through 3; for two additional pretest high-dose level preparations; and for four additional pretest low-dose level preparations. One sample (approximately 100 g) taken from the top, middle, and bottom of the mix were collected, divided into three subsamples for extraction and analysis (see Protocol Deviations for exceptions), and analyzed for test material content. All samples were stored at room temperature. To evaluate the stability of the test material in the carrier, four sets of samples (approximately 100 g each) were taken from the low- and high-dose level concentrations of diet preparations mixed pretest. One set was analyzed on the day of mixing; one set was analyzed after storage at room temperature for 19 days; one set was analyzed after storage at room temperature for 34 days; and the remaining set was analyzed after being stored for 8 weeks in a freezer set to maintain -10 to -30oC. In addition, samples (approximately 100 g each) were taken from the low- and high-dose level concentrations of diet preparations mixed pretest. The samples were stored at room temperature for 5 days, then for 7 days under animal room conditions (room temperature in a feeding container), then analyzed. For dose confirmation, samples (approximately 100 g each) from all dose preparations were analyzed in duplicate. Homogeneity samples collected from the middle of the dose preparations for Weeks 1 through 3 were used for dose confirmation results. All samples were stored at room temperature until analyzed. Observation of Animals Clinical Observations. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity (see Protocol Deviations for exceptions). Once prior to treatment and weekly thereafter, each animal was removed from its cage and examined; abnormal findings or an indication of normal were recorded (see Protocol Deviations for exceptions). In addition, the time of onset, location, size, appearance, and progression of each grossly visible or palpable mass were recorded. 23 Covance 6329-212 3M T-6316.1 Body Weights. Individual body weight data were recorded on Day -4 (randomization), weekly for Weeks 1 through 17, once every 4 weeks thereafter, and at Week 105 (see Protocol Deviations for exceptions). Body weights were also recorded for animals in Groups 5 and 7 before sacrifice during Week 8. Food Consumption. Individual food consumption data were recorded weekly for Weeks 1 through 16 and once every 4 weeks thereafter (see Protocol Deviations for exceptions). Clinical Pathology Blood and urine samples were collected from five animals/sex/group from Groups 1 and 5 during Week 8 and from 10 animals/sex/group from Groups 1 through 4 during Weeks 14, 27, and 53. Blood films were made and held for possible future examination from animals sacrificed at unscheduled intervals and from animals at scheduled sacrifices after at least 52 and 104 weeks of treatment (see Protocol Deviations for exceptions). In addition, blood was collected for cholesterol and triglycerides tests only from all remaining animals at the terminal and recovery sacrifices. Animals were fasted overnight, and urine was collected chilled for approximately 16 hours before blood sampling; water was provided a d libitum. Blood was collected from a jugular vein. The anticoagulant was potassium EDTA for the hematology tests. Samples for clinical chemistry and serum samples were collected without anticoagulant. Animals were bled in random order. The following were evaluated. Hematology red blood cell (erythrocyte) count hemoglobin hematocrit mean corpuscular volume mean corpuscular hemoglobin mean corpuscular hemoglobin concentration platelet count white blood cell (leukocyte) count differential blood cell count segmented neutrophil count lymphocyte count monocyte count eosinophil count basophil count blood cell morphology Reticulocyte smears were made and held for possible future examination. 24 glucose urea nitrogen creatinine total protein albumin globulin cholesterol total bilirubin Covance 6329-212 3M T-6316.1 Clinical Chemistry alanine aminotransferase gamma glutamyltransferase aspartate aminotransferase calcium inorganic phosphorus sodium potassium chloride appearance volume specific gravity pH protein urobilogen Urinalysis glucose ketones bilirubin blood microscopic examination of sodium potassium Urine Chemistry 16-hour excretion of: sodium potassium Serum samples not used for clinical chemistry were stored in a freezer set to maintain -60 to -80oC. Samples were shipped on dry ice to the Sponsor for possible future analysis. Serum Analyses Blood [approximately 2 mL at the Week 4, 8, and 14 sacrifices and approximately 3 mL at the Week 53 and 105 sacrifices (see Protocol Deviations for exceptions)] was collected. Samples were collected from five animals/sex/group in Groups 1 through 5 during Week 4 (animals selected for hepatocellular proliferation and biochemical analyses); five animals/sex/group in Groups 1 and 5 during Week 8; five animals/sex/group in Groups 1 through 4 during Week 14 (animals selected for hepatocellular proliferation and biochemical analyses); five animals/sex/group from Groups 1 and 4 (from animals selected for interim sacrifice) after at least 52 weeks of treatment; and all remaining animals at terminal and recovery sacrifices. 25 Covance 6329-212 3M T-6316.1 Animals were fasted overnight and blood was collected without anticoagulant from a jugular vein. Blood samples were allowed to clot at room temperature, then centrifuged. Serum samples were harvested and stored in a freezer set to maintain -60 to -80oC. Samples were shipped on dry ice to the Sponsor for possible future analyses. Anatomic Pathology Necropsy. A necropsy was done on each animal that died or was sacrificed at an unscheduled interval. Animals sacrificed before Week 13 were anesthetized with sodium pentobarbital and animals sacrificed during or after Week 13 were anesthetized with carbon dioxide (see Protocol Deviations for exceptions). During Week 4, five animals/sex/group from Groups 1through 5 were fasted overnight, anesthetized with sodium pentobarbital, weighed, and exsanguinated. The liver was removed and weighed. Representative samples of left lateral, right median, and right lateral lobes and any macroscopic lesions of the liver were collected and preserved in zinc formalin for proliferation cell nuclear antigen (PCNA). Liver samples were also collected for palmitoyl CoA analysis. A sample (approximately 1.5 g) from the remaining portion of the liver was collected from selected animals. Samples were placed on wet ice and transferred to the Sponsor for mitochondrial analysis. Results will not be included in this report. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and sent to the Sponsor for possible future analysis. During Week 8, five animals/sex/group from Groups 1 and 5 were fasted overnight, bled for clinical pathology and serum analyses, anesthetized with sodium pentobarbital, weighed, exsanguinated, and necropsied. Liver samples were collected for palmitoyl CoA analysis and PCNA evaluation. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. The remaining animals in Groups 5 and 7 were euthanatized with carbon dioxide and the carcasses discarded without necropsy. During Week 14, five animals/sex/group from Groups 1through 4 were fasted overnight, bled for clinical pathology and serum analyses, anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Liver samples were collected for palmitoyl CoA analysis and PCNA evaluation. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. 26 Covance 6329-212 3M T-6316.1 After at least 52 weeks of treatment, 5 animals/sex from Group 1 and 10 animals/sex from Group 4 were fasted overnight, anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. After 104 weeks of treatment, the remaining animals in Groups 1 through 4 were fasted overnight, then anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. After 52 weeks of treatment and 52 weeks without treatment, the remaining animals in Group 6 were fasted overnight, then anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. Additional liver samples were stored in a freezer, set to maintain -60 to -80oC, and shipped to the Sponsor for possible future analysis. The necropsy included a macroscopic examination of the external features of the carcass; external body orifices; the abdominal, thoracic, and cranial cavities; organs; and tissues. Proliferation Cell Nuclear Antigen Evaluation Liver samples were collected from five animals/sex/group in Groups 1through 5 during Weeks 4 and 8 and five animals/sex/group in Groups 1 through 4 during Week 14. At the Week 4 collection, representative samples of left lateral, right median, and right lateral lobes of the liver and macroscopic lesions of the liver were collected and preserved in zinc formalin. At the Week 8 and 14 collections, representative samples of the left lateral lobe and any macroscopic lesions of the liver were collected and preserved in zinc formalin. After fixation, each sample of the liver was embedded in paraffin, and the paraffin blocks were shipped to Pathology Associates International. Proliferation cell nuclear antigen (PCNA) evaluation were done on the samples (left lateral lobe only). In addition, sections of the left lateral lobe of the liver were stained with hematoxylin and eosin and examined microscopically. 27 Covance 6329-212 3M T-6316.1 Palmitoyl-CoA Oxidase Tissue Collection and Analyses Liver samples were collected from five animals/sex/group in Groups 1through 5 during Week 4; five animals/sex/group in Groups 1through 5 during Week 8; and five animals/sex/group in Groups 1through 4 during Week 14. A sample (approximately 500 mg) of the right lateral lobe of the liver was also collected from each animal and flash-frozen in liquid nitrogen (see Protocol Deviations for exceptions). The liver tissue was stored in a freezer, set to maintain -60 to -80oC, until analyzed for palmitoyl CoA oxidase activity. Organ Weights. At the interim sacrifices during Weeks 8, 14, and 53, the following organs (when present) were weighed; paired organs were weighed separately (see Protocol Deviations for exceptions). adrenal (2) brain kidney (2) liver lung ovary (2) spleen testis (2) thyroid (2) with parathyroid uterus with cervix Organ-to-body weight percentages and organ-to-brain weight ratios were calculated. Tissue Preservation. The following tissues were collected and preserved in 10% neutral-buffered formalin (see Protocol Deviations for exceptions). adrenal (2) brain cecum cervix colon duodenum epididymis (2) esophagus eye (2) femur with bone marrow (articular surface of the distal end) Harderian gland heart ileum jejunum kidney (2) lesions liver lung with mainstem bronchi lymph node (mesenteric) mammary gland (females only) ovary (2) pancreas pituitary prostate rectum salivary gland [mandibular (2)] sciatic nerve 28 seminal vesicle (2) skeletal muscle (thigh) skin spinal cord (cervical, thoracic, and lumbar) spleen sternum with bone marrow stomach Covance 6329-212 3M T-6316.1 testis (2) thymus thyroid (2) with parathyroid trachea urinary bladder uterus vagina Bone marrow smears from the femur of each animal at scheduled sacrifices were prepared, stained with Wright's stain, and retained for possible examination. In addition, at the terminal and recovery sacrifices, a section of the heart and liver were collected from 10 animals/sex in Groups 1, 3, 4 (terminal sacrifice), and 6 (recovery sacrifice) and preserved in 2.0% paraformaldehyde/2.5% glutaraldehyde in 0.1M phosphate buffer. The tissues were processed and embedded in epoxy blocks for possible future examination by electron microscopy. Histopathology. Tissues (as appropriate) were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically from each animal in Groups 1 and 4 sacrificed at the terminal sacrifice and any animals that died on test or were sacrificed at unscheduled intervals. Tissues (as appropriate) from each animal in Groups 1 and 4 sacrificed at the Week 53 interim sacrifice were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, mammary glands (females), and urinary bladder from each animal in Groups 2, 3, and 6 sacrificed at the terminal or recovery sacrifices were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. Adrenals, brain, eyes, kidneys, liver, mesenteric lymph nodes, pancreas, spleen, testes, and ovaries from the animals necropsied at the Week 8 and 14 interim sacrifices were also embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically. A pathology peer review was conducted by a pathologist designated by the sponsor. 29 Covance 6329-212 3M T-6316.1 Liver sections from five animals/sex/group from Groups 1 and 4 were stained with Oil Red "O" stain and examined microscopically. Liver sections in epoxy blocks for five animals/sex/group from Groups 1 and 4 were processed and evaluated by electron microscopy by Pathology Associates International (report included in Appendix 8). Statistical Evaluation Levene's test (Levene, 1960) was done to test for variance homogeneity. In the case of heterogeneity of variance at p < 0.05, transformations were used to stabilize the variance. One-way analysis of variance [ANOVA (Winer, 1971)] was used to analyze initial body weights, body weight changes, food consumption, continuous clinical pathology values, and organ weight data. ANOVA was done on the homogeneous or transformed data. If the ANOVA was significant, Dunnett's t-test (Dunnett, 1964) was used for pairwise comparisons between treated and control groups. Group comparisons were evaluated at the 5.0%, two-tailed probability level. Only data collected on or after the first day of treatment were analyzed statistically. Survival and Tumor Analysis Survival. Evaluations of trend and heterogeneity of survival data were performed using the Cox-Tarone binary regression method and the National Cancer Institute (NCI) Life Table Package (Thomas, Breslow, and Gart, 1977). Week 105 was treated as the end of the study in the NCI package for both sexes. Animals that died accidentally and those that were sacrificed at scheduled intervals were censored in the analysis. Continuity corrected one-sided tail probabilities for trend and two-sided tail probabilities for group comparisons were evaluated at the 5.0% significance level. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence rate in at least one treated group was increased or decreased by at least two occurrences over the control group. These were analyzed by the Cochran-Armitage test for trend and the Fisher-Irwin exact test for control versus treatment comparisons 30 Covance 6329-212 3M T-6316.1 (Thakur, Berry, and Mielke, 1985). One-sided tail probabilities for trend and group comparisons were evaluated at the 5.0% significance level. Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses if the incidence rate in at least one treated group was increased or decreased by at least two occurrences over the control group. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). The logistic regression model included linear and quadratic terms in age (week of death), but the quadratic term was eliminated if there was a lack of convergence. The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method, as in the case of survival, using the death time or the first palpation time (as applicable) as the tumor onset time. In the case of any particular tumor type where the study pathologist assigned the tumor in question as being the cause of death of a subset of the animals and the rest of the animals were assumed to be dead of other competing risks, IARC-type (Peto, et al., 1980) cause of death analysis was performed. Specifically, the subset of the tumors, which were assigned to be the cause of death by the examining pathologist, was analyzed by Cox-Tarone logistic regression under life table techniques. The subset, which was considered incidental by the pathologist, was analyzed by logistic regression of tumor prevalences. For the cases of tumor types where cause of death was undetermined, these were treated as incidental for statistical evaluation. The score statistics and their respective variances from the above tests were then used to compute the combined evidence as described by Gart, et al., (1986). In the case of only one tumor belonging to one of the two categories (fatal and incidental) in a test, it was combined with the other category for the purpose of statistical analyses. In addition, in cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the test was obtained by using LogXact-Turbo (1993) and combined with the probability of the Cox-Tarone test, if necessary. Common and rare tumors were evaluated at the 1.0% and 5.0% significance levels, respectively, for trend. The benign and malignant neoplastic incidences were evaluated separately, but were combined where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as hemangioma, hemangiosarcoma, lipoma, liposarcoma, fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, endometrial stromal polyp, and endometrial stromal sarcoma were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may 31 Covance 6329-212 3M T-6316.1 be biased because not all the animals were examined for every tissue. Pairwise one-sided group comparisons were evaluated at the 5.0% significance level. Groups 5 (High-dose) and 7 (High recovery) were excluded from all the analyses except the survival graphs due to early sacrifices at Week 8. In the nonneoplastic analyses, animals sacrificed early (Week 4) in Groups 1 through 4 (Control, Low, Mid, and Mid-High, respectively) were excluded from the denominators. In the statistical evaluations of the neoplastic lesions, animals sacrificed early (during or before Week 14) in Groups 1 through 4 were discounted from the number of animals at risk. Further, in the neoplastic analyses, in the cases where the low, mid, and mid-high recovery groups did not have a complete histopathology examination, they were excluded from statistical analyses. The p-values under Group 1 (Control) are for trend analysis and the p-values under Groups 2 (Low), 3 (Mid), 4 (Mid-High), and 6 (Mid-High recovery) are for the comparison of that group versus the control group. Group 6 was also compared with Group 4. Trend analysis includes Groups 1, 2, 3, and 4 only. Ordinal dose levels 0, 1, 2, and 3 were used in all analyses for Groups 1, 2, 3, and 4, respectively. Continuity correction was done for all asymptotic tests. The following notations are used throughout this report to designate direction and significance of effects. + = Effect in the increased direction - = Effect in the decreased direction * = Significant at p < 0.05 ** = Significant at p < 0.01 E = Exact permutation test NA = Not analyzed Record Retention The raw data, documentation, records, specimens, protocol, and final report generated as a result of this study will be archived in the storage facilities of Covance-Madison. At least 1 year after submission of the final report, the Covance Archives staff will contact the Sponsor. At that time, the Sponsor may choose to have the aforementioned materials returned, archived for an additional period of time, or disposed of by Covance; a fee will 32 Covance 6329-212 3M T-6316.1 be charged based on the archive disposition option selected by the Sponsor. Raw data stored on magnetic media and the protocol, study correspondence, and the original final report will be retained by Covance. At least 1 year after submission of the final report, any frozen samples will be returned to the Sponsor, retained by Covance, or disposed of, and a fee will be charged. Data generated by the Sponsor will be archived in the Sponsor's storage facilities. Specimens, data, and reports for PCNA evaluation and electron microscopy will be retained by Pathology Associates International. Frozen liver samples and serum samples sent to the Sponsor will be retained by the Sponsor. RESULTS AND DISCUSSION Dose Analysis Results of the homogeneity, stability, and dose preparation analyses are in Tables 1 through 3. Prestudy (September 11, 29, October 15, and November 7 and 13, 1997), several homogeneity analyses were conducted on each of several mixes in an attempt to resolve issues related to the inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring N-EtFOSE. Mean values of the homogeneity analyses for the mix done on January 15, 1998, ranged from 89.7-104%, 92.0-98.7%, 86.8-98.4%, and 94.0-96.7% of the theoretical concentrations for the diets containing 3, 30, 100, or 300 ppm N-EtFOSE, respectively. Mean values of an additional homogeneity analyses for the mix done on August 26, 1999, ranged from 66.2 to 72.7% and 121 to 136% of the theoretical concentrations for the diets containing 100 or 300 ppm, respectively. Prestudy (September 11 and 29, 1997), several stability analyses were conducted on each of several mixes in an attempt to resolve the issues related to the inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring N EtFOSE. Results of the stability analyses for the mix conducted on October 15, 1997, of samples stored for 19 days at room temperature conditions indicated that the mean concentrations were 99.3 and 86.7% of the theoretical concentrations of 3 and 300 ppm, respectively. Results of the stability analyses for the mix conducted on October 15, 1997, of samples stored for 34 days at room temperature conditions indicated that the mean concentrations were 100 and 88.7% of the theoretical concentrations of 3 and 300 ppm, respectively. Results of stability analyses for the mix conducted on October 15, 1997, of 33 Covance 6329-212 3M T-6316.1 samples stored for 8 weeks under frozen conditions indicated that the mean concentrations were 99.0 and 94.3% of the theoretical concentrations of 3 and 300 ppm, respectively. Results of the stability analyses for an additional mix conducted November 13, 1997, of samples stored 5 days at room temperature followed by 7 days under animal room conditions indicated that the mean concentrations were 118 and 87.7% of the theoretical concentrations of 3 and 300 ppm, respectively. The mean concentrations of the dose preparation analyses for all levels ranged from 33.3 to 158% of the theoretical concentrations (including the reassay and retention sample analyses, but excluding data that required reanalysis.). Inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring N-EtFOSE resulted in homogeneity, stability, and routine analyses data that were in many cases outside of the standard limits o f+15%. Observation of Animals Survival and Clinical Observations. Clinical observations including mass observations are summarized in Tables 5 and 6; individual data are in Appendix 2. Survival data are illustrated in Figures 1 and 2 and summarized in Table 4; individual animal fate data are in Appendix 2. Thirty-six control males and 37 control females, 28 males and 37 females given 3 ppm, 28 males and 35 females given 30 ppm, 33 males and 30 females given 100 ppm for 104 weeks (Group 4) and 30 males and 28 females given 100 ppm for 52 weeks (Group 6) died on test or were sacrificed in a moribund condition. There were no test material-related effects on mortality. The most frequent cause of death was pituitary adenoma. Clinical observations were similar in nature and in the total number of affected animals in all groups. Body Weights. Body weight and body weight change data are illustrated in Figures 3 and 4 and summarized in Tables 7 and 8; individual body weight data are in Appendix 3. There was a dose-related decrease on body weights for males and females given 30 or 100 ppm. Mean cumulative body weight change was statistically significantly lower for males given 30 or 100 ppm diets for Weeks 1 through 15 and 15 through 29. Mean body 34 Covance 6329-212 3M T-6316.1 weight change was statistically significantly lower for females given 30 or 100 ppm for Weeks 1 through 15 and for Weeks 29 to 57 and for females given 100 ppm for Weeks 15 through 29. There was an increase in the rate of body weight gain for Group 6 animals after they were placed on recovery after 52 weeks of treatment. Food Consumption. Food consumption data are illustrated in Figures 5 and 6 and summarized in Table 9; individual data are in Appendix 4. Mean food consumption for males and females fed the 100- or 300-ppm diets as well as the males fed the 30-ppm diet was generally lower compared with that of the control group, and these values were frequently statistically significantly different from control values. Mean food consumption for males fed the 3-ppm diets and females fed the 3- or 30-ppm diets were comparable to controls with only sporadic differences in mean food consumption noted. Test Material Consumption. Test material consumption data are summarized in Table 10. Individual test material consumption data are presented in Appendix 4. Animals were fed diets containing 3, 30, 100, or 300 ppm N-EtFOSE The amount of test material consumed by the animals on a mg/kg of body weight/day basis was as follows. Group 2 3 4 5/7 Target Dose Levels (ppm) 3 30 100 300 Achieved Dose Levels - Range (mg/kg body weight/day) Males Females 0.259 -0.081 0.109 -0.307 0.860 -2.618 1.029 -3.070 3.065 - 8.724 4.213 - 10.166 15.803-25.408 19.568 -29.270 Clinical Pathology Hematology, coagulation, clinical chemistry, urinalysis, and urine chemistry data are summarized in Tables 11 through 28; individual data are in Appendix 5. The Pathology Report contains a discussion of the data. Dietary administration of N-EtFOSE for approximately 52 weeks was associated with mildly decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; mildly lower glucose and mildly higher urea nitrogen for males and females 35 Covance 6329-212 3M T-6316.1 given 100 ppm (predominantly at Weeks 14 and 27); moderately higher albumin and moderately lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and moderately to markedly lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. The effect on cholesterol was reversed following the 52-week recovery. Administration of N-EtFOSE at 3 ppm for approximately 52 weeks had no effects on clinical pathology test results. Administration of N-EtFOSE for approximately 104 weeks was associated with moderately lower cholesterol for males given 30 or 100 ppm and moderately lower triglycerides for males and females given 100 ppm. At a dose level of 300 ppm for approximately 8 weeks, administration of N-EtFOSE was associated with many effects on clinical pathology results including lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase (also observed at Week 4). Most of the effects were moderate to marked in magnitude. Anatomic Pathology Organ weight data are summarized in Tables 29 through 32; incidences of macroscopic and microscopic observations are summarized in Tables 33 through 45; the incidence of severity of selected microscopic observations is summarized in Tables 46 through 51. Individual data are in Appendix 6. The Pathology Report contains a discussion of the data. At the interim sacrifices, body weights were lower and absolute and relative liver weights were higher for animals given 100 or 300 ppm of the test material. Macroscopically, diffusely dark livers were only observed for animals given 100 ppm of the test material. Enlargement, mottling, and masses of the liver were slightly more frequent for animals given 100 ppm than for the controls. Administration of the test material caused an increase in cystic degeneration, hepatocellular hypertrophy, hepatocellular vacuolation, necrosis and hemorrhage of the liver. 36 Covance 6329-212 3M T-6316.1 At the Week 8 sacrifice, hepatocellular hypertrophy, necrosis and hemorrhage were observed for animals given 300 ppm. Hepatocellular hypertrophy was observed only at the Week 14 sacrifice for animals given 30 or 100 ppm, and hepatocellular vacuolation and hypertrophy were present at the Week 53 sacrifice only for animals given 100 ppm. The hepatic changes were considered toxicologically important. In males, combined thyroid follicular cell adenoma/carcinoma was significantly increased as compared to controls at 100 ppm (p = 0.011) which was due primarily to an increase in follicular cell adenoma (p = 0.011). Thyroid follicular cell carcinoma alone was not increased. The relationship of this finding to treatment is questionable for several reasons: thyroid follicular cell tumors in historical controls from this laboratory are relatively common (occur with an incidence of greater than 1%), however, no thyroid follicular cell tumors occurred in male controls from this study, there was no evidence on nonneoplastic lesions of the thyroid, the increase at 100 ppm was within the range of historical controls, and a clear dose-response relationship was not present. In females, the incidence of combined hepatocellular adenoma/carcinoma was significantly increased in the 100 ppm dose group as compared to controls (p = 0.007) as a result of a significant increase in hepatocellular adenoma in this group (p = 0.012). There was no increase for trend or pairwise for hepatocellular carcinoma alone. The only hepatocellular carcinoma observed in this study was in a 100-ppm, terminal-sacrifice female. Recovery females in the 100 ppm dose group experienced a marginally significant increase in hepatocellular adenoma (p = 0.05). It is noteworthy that, in females, liver effects were only observed at 100 ppm and included the finding of a hepatocellular adenoma at the Week 53 sacrifice. Significant negative trends and pairwise decreases at 100 ppm occurred among females for mammary tumors (fibroadenoma, combined fibroadenoma/adenoma, and combined fibroadenoma/adenoma/carcinoma). Trends for pituitary adenoma and combined adenoma/carcinoma were also negative in females. Proliferation Cell Nuclear Antigen Results A statistically significant increase in proliferative index was observed transiently in males of the 300 ppm dose group at Week 4, but not at Week 8 or in any other male dose groups. Biological significance for an individual animal is defined as a two-fold or greater increase in proliferative index (PI) over the averaged control values, and the PI must be greater than the highest control value. Biologically significant increases in PI 37 Covance 6329-212 3M T-6316.1 were observed in one out of five males of the 100 ppm dose group at Week 4, however, these increases were not sustained through Week 8. Biologically significant increases in PI were observed in two out of five males at Week 4 and in two out of five males in the 300 ppm dose group at Week 8. No increase in hepatocellular proliferation was observed at Week 14 in the male 100 ppm dose group. The NOEL for hepatocellular proliferation in males was the 100 ppm dose group. Cell proliferation was not statistically or biologically significant in females at 4, 8, or 14 weeks on study in any dose group. The NOEL for hepatocellular proliferation in females was the 300 ppm dose group. Electron Microscopy Results Liver samples were obtained from each of 20 animals (10 animals/sex/group from the control and mid-high dose groups) during necropsy at terminal sacrifice for examination by transmission electron microscopy (TEM). Liver samples were assessed for changes that may likely occur due to chronic toxicity of the test material. Electron microscopic evaluation identified mild to moderate changes related to treatment. Mild to moderate smooth endoplasmic reticulum hyperplasia and minimal to mild hepatocellular hypertrophy were the prominent features found to be different between the mid-high dose group and control animals. Other changes included a slight increase in the amount of lipid accumulation and minor increases in lipofuscin were found in the treated animals compared with controls. CONCLUSION Male and female Crl:CD(SD)IGS BR rats were administered N-Ethyl Perfluorooctanesulfonamido Ethanol at doses of 0, 3, 30 and 100 ppm at least 104 weeks. There were no significant effects on survival, and the incidence of hepatocellular adenomas was slightly higher for males and females given 100 ppm. The only hepatocellular carcinoma in the study was present for a terminal sacrifice female given 100 ppm. 38 SIGNATURES Covance 6329-212 3M T-6316.1 39 Covance 6329-212 3M T-6316.1 REFERENCES Dunnett, C. W., "New Tables for Multiple Comparisons with a Control," B iom etrics, 20:482-491 (1964). Levene, H., "Robust Tests for Equality of Variances," Contributions to P robability an d Statistics, (eds.) I. Olkin et al., Ch. 25, pp. 278-292, Stanford University Press: Stanford, California (1960). Winer, B. J., "Design and Analysis of Single-Factor Experiments," S tatistical Principles in Experim ental D esign, Second Ed., Ch. 3, pp. 149-260, McGraw-Hill: New York, New York (1971a). Winer, B. J., "Analysis of Covariance," S tatistical P rin ciples in Experim ental D esign, Second Ed., Ch. 10, pp. 752-812, McGraw-Hill: New York, New York (1971b). 40 PATHOLOGY REPORT Covance 6329-212 3M T-6316.1 SUMMARY The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE), when administered in the diet to rats for at least 104 weeks. The test material was administered at dose levels of 0, 3, 30, 100, and 300 ppm. Animals given 300 ppm were sacrificed during Week 8. Dietary administration of N-EtFOSE for approximately 52 weeks was associated with mildly decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; mildly lower glucose and mildly higher urea nitrogen for males and females given 100 ppm (predominantly at Weeks 14 and 27); moderately higher albumin and moderately lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and moderately to markedly lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. The effect on cholesterol was reversed following the 52-week recovery. Administration of N-EtFOSE at 3 ppm for approximately 52 weeks had no effects on clinical pathology test results. Administration of N-EtFOSE for approximately 104 weeks was associated with moderately lower cholesterol for males given 30 or 100 ppm and moderately lower triglycerides for males and females given 100 ppm. At a dose level of 300 ppm for approximately 8 weeks, administration of N-EtFOSE was associated with many effects on clinical pathology results including lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase (also observed at Week 4). Most of the effects were moderate to marked in magnitude. At the interim sacrifices, body weights were lower and absolute and relative liver weights were higher for animals given 100 or 300 ppm of the test material. Macroscopically, diffusely dark livers were only observed for animals given 100 ppm of the test material. Enlargement, mottling, and masses of the liver were slightly more frequent for animals given 100 ppm than for the controls. 41 Covance 6329-212 3M T-6316.1 Administration of the test material caused an increase in cystic degeneration, hepatocellular hypertrophy, hepatocellular vacuolation, necrosis and hemorrhage of the liver. At the Week 8 sacrifice, hepatocellular hypertrophy, necrosis and hemorrhage were observed for animals given 300 ppm. Hepatocellular hypertrophy was observed only at the Week 14 sacrifice for animals given 30 or 100 ppm, and hepatocellular vacuolation and hypertrophy were present at the Week 53 sacrifice only for animals given 100 ppm. The hepatic changes were considered toxicologically important. Test material related hepatic findings for animals given 100 ppm at the terminal sacrifice were cystic degeneration (males only) and hepatocellular hypertrophy and vacuolation. The incidence of hepatocellular adenomas was slightly higher for males and females given 100 ppm. The higher incidence was statistically significant for females. The only hepatocellular carcinoma in the study was present for a terminal sacrifice female given 100 ppm. The incidence of hepatocellular adenomas and carcinomas is listed in Text Table 2. METHODS Seven groups of Crl:CD(SD)IGS BR rats were studied using the following study design. 42 Covance 6329-212 3M T-6316.1 No. of Animals Dietary Levels a Group Male Female (ppm N-EtFOSE) 1 (Control) b 70 70 0 2 (Low) 60 60 3 3 (Mid) 60 60 30 4 (Mid-High) 70 70 100 5 (High)c 70 70 300 6 (Mid-High Recovery) 40 40 100 7 (High Recovery)0 40 40 300 8 70 70 0 9 70 70 1 a T-6316 is 98.1% n-ethyl perfluorooctanesulfonamido ethanol (N-etFOSE); dose levels are expressed as ppm of N-EtFOSE. b The control animals received the basal diet only. c Five animals/sex in Group 5 were sacrificed during Week 8; the remaining animals in Groups 5 and 7 were sacrificed and discarded during Week 8. During Week 8, blood and urine were collected for hematology, clinical chemistry, urinalysis, and urine chemistry tests from five animals/sex/group in Groups 1 and 5. During Weeks 14, 27, and 53, blood and urine were collected for these tests from 10 animals/sex/group in Groups 1 through 4. Blood smears were made for all animals sacrificed at unscheduled intervals or during Weeks 53 (interim sacrifice) and 105 (terminal and recovery sacrifices). During Week 105, blood was collected for cholesterol and triglycerides from all surviving animals. Necropsies were performed on five animals/sex in Groups 1 and 5 sacrificed during Week 8; five animals/sex in Groups 1 through 4 sacrificed during Week 14, five animals/sex in Group 1 and 10 animals/sex in Group 4 sacrificed during Week 53, and all remaining surviving animals during Week 105. At necropsy, macroscopic observations were recorded, organ weights were obtained (for sacrifices during Weeks 8, 14, and 53), and tissues were placed in fixative as specified by the protocol. At unscheduled necropsies for animals that died or were sacrificed because of poor health, macroscopic observations were recorded, and tissues were placed in fixative, but organ weights were not obtained. Microscopic examinations were done on selected tissues from animals in Groups 1 through 5 necropsied during Weeks 8 and 14. The tissues were adrenals, brain, eyes, kidneys, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries. Microscopic examinations were done on all collected tissues from animals in Groups 1and 4 necropsied at the Week 53 interim sacrifice and the Week 105 terminal sacrifice and from animals that died or were sacrificed due to poor health. In addition, 43 Covance 6329-212 3M T-6316.1 microscopic examinations were done on macroscopic lesions, liver, lungs, kidney, pancreas, thyroid, testes, and mammary glands (females) from animals in Groups 2, 3, and 6 sacrificed during Week 105. Five animals/sex in Groups 1 through 5 were sacrificed during Week 4, and five animals/sex in Groups 1 through 4 were sacrificed during Week 14 for hepatocellular proliferation rate measurements and hepatic palmitoyl-CoA oxidase activity. Statistically significant differences cited in the Results and Discussion section are based on comparisons between the control and treated groups. RESULTS AND DISCUSSION Mortality The incidence of mortality during the study was similar for treated and control animals. Thirty-six males and 37 females in the control group, 28 males and 37 females given 3 ppm, 28 males and 35 females given 30 ppm, and 33 males and 31 females given 100 ppm died on test or were sacrificed in moribund condition. Clinical Pathology Week 4. Males and females given 300 ppm had mildly, but statistically, higher hepatic palmitoyl-CoA oxidase activity consistent with peroxisomal proliferation. Week 8. There were many statistically significant or otherwise notable differences for clinical pathology results between animals given the control material and animals given 300 ppm. Those differences considered to be effects of the test material were lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase. With the exception of higher alanine aminotransferase for the females, these differences were statistically significant. The effects on the erythrocyte parameters, glucose, and globulin were consistent with poor health and failure to gain weight appropriately. Higher urea nitrogen and albumin were most consistent with mild dehydration and indicated the effects on other parameters (e.g., the erythrocyte parameters) would likely have been greater if the animals were normovolemic. The effects on alanine aminotransferase, aspartate 44 Covance 6329-212 3M T-6316.1 aminotransferase, and total bilirubin were indicative of the hepatocellular degeneration and necrosis observed microscopically. Other statistically significant differences were considered to be of uncertain relationship to administration of the test material. Mildly lower platelet count was observed for males but not females. Absolute eosinophil count was statistically lower for both males and females, but the differences were extremely small as normal eosinophil count is very low. Weeks 14, 27, and 53. There were several statistically significant or otherwise notable differences for clinical pathology results between control and treated animals. Those differences considered to be effects of the test material were not always statistically significant but were generally consistent over time and exhibited a relationship to dose. Several statistically significant differences that were inconsistent over time or did not affect the high-dose group were considered incidental. Administration of the test material was associated with decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; lower glucose and higher urea nitrogen for males and females given 100 ppm (predominantly at Weeks 14 and 27); higher albumin and lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. These effects were consistent with those observed at Week 8 for animals given 300 ppm but were less prominent. The effect of greatest magnitude was that for cholesterol. At the highest dose level, cholesterol was markedly decreased for the males. The erythrocyte effects and the effects on glucose and urea nitrogen were very mild, and the serum protein effects were considered of moderate severity. Administration of the test material at 3 ppm for approximately 52 weeks had no effect on clinical pathology test results. Week 105. Cholesterol remained moderately lower for males given 30 or 100 ppm (Groups 3 and 4), and triglycerides were moderately lower for females given 100 ppm (Group 4). These effects were absent or reversed for animals in the recovery group (Group 6). 45 Covance 6329-212 3M T-6316.1 Anatomic Pathology - All Animals - Test Material-Related Findings Macroscopic Findings. Diffusely dark livers were observed only for animals given the test material. Enlargement, mottling, and masses of the liver were slightly more frequent for animals given 100 ppm than for the controls. An increased incidence of light foci of the liver for treated animals was not considered test material-related as most of these were due to tension lipidosis, an incidental finding frequently observed in rats. The incidence of possible test material-related macroscopic findings are listed in Text Table 1. Text Table 1 Incidence of Possible Test Material-Related Macroscopic Findings N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 100a 0 3 30 100 Males Females Animals Examined 70 60 70 40 70 60 60 70 Liver Light Focus (I)/Area(s) 0 4 2 5 4 032 6 Large 14 1 4 1 110 3 Mottled 53 9 6 1 32 1 8 Diffusely Dark 00 0 5 1 000 7 Masses 22 1 3 0 0 10 3 a Animals placed on recovery. 100a 40 6 0 4 1 1 Microscopic Findings. Test material-related findings were limited to the liver. Administration of the test material caused an increase in cystic degeneration, hepatocellular hypertrophy, hepatocellular vacuolation, necrosis, and hemorrhage. At the Week 8 sacrifice, hepatocellular hypertrophy, necrosis, and hemorrhage were observed for animals given 300 ppm. Hepatocellular hypertrophy was observed only at the Week 14 sacrifice for animals given 30 or 100 ppm, and hepatocellular vacuolation and hypertrophy were present at the Week 53 sacrifice only for animals given 100 ppm. For the unscheduled sacrifice animals, cystic degeneration, and hepatocellular hypertrophy were increased for males and hepatocellular vacuolation was increased for treated males and females. Hepatocellular adenomas were minimally higher for treated animals compared with the controls. 46 Covance 6329-212 3M T-6316.1 Test material-related hepatic findings for animals given 100 ppm at the terminal sacrifice were cystic degeneration (males only), hepatocellular hypertrophy, and vacuolation. Livers from five males, five females in the control group, and five males and five females given 100 ppm were stained with Oil Red O for lipid. The amount of lipid present was slightly higher for males given 100 ppm than for the control males. The incidence and amount of lipid present for control males was one minimal and three slight and for males given 100 ppm it was one minimal, two moderate, and one moderately severe. There was no difference in the amount of lipid present for the females. The incidence of hepatocellular adenomas was slightly higher for males and females given 100 ppm. There was only one hepatocellular carcinoma in the study, and it was present for a terminal sacrifice female given 100 ppm. The microscopic findings for the Group 6 sacrifice animals given 100 ppm were similar to the controls. However, 2 of the 12 females had hepatocellular adenomas. The incidence of test material-related microscopic findings for all animals are listed in Text Table 2. T ext Table 2 Incidence o f T est M aterial-R elated M icroscopic Findings 0 A nim als Exam ined L iv er C ystic D egeneration H epatocellular H ypertrophy H epatocellular V acuolation H epatocellular A denom a H epatocellular C arcinom a a A nim als placed on recovery. 65 6 0 17 2 0 N -E thyl Perfluorooctanesulfonam ido (ppm ) 3 30 100 100a 0 3 30 100 M ales Fem ales 55 55 65 40 65 55 55 65 8 14 18 1 3 22 18 23 39 425 000 7 2 11 0 0 02 10 13 14 01 00 02 0 14 8 26 36 01 100a 40 1 0 8 3 0 Anatomic Pathology - All Animals - Nontest Material-Related Findings Macroscopic Findings. The most frequently observed findings at necropsy other than those observed associated with test material-related effects were ventral compression of the brain, pituitary masses, ocular discharge, perineal staining, and malocclusion. Other frequent incidental macroscopic findings for males were red foci in the liver, fluid contents in the gastrointestinal tract, obesity, nodular prostate, and nasal discharge. 47 Covance 6329-212 3M T-6316.1 Frequently observed nontest material-related findings for females were mammary masses, thickened mammary glands, thin animals, ovarian cyst, uterine cyst, stained hair coat, and dark foci in the stomach. Microscopic Findings/Neoplastic Findings. The most frequently observed nontest material-related neoplasms were pituitary adenomas, and c-cell adenomas and follicular cell adenomas of the thyroid. Mammary adenomas, fibroadenomas, and carcinomas were frequently present for females. Other tumors were only present in a few animals, and the only rare tumor in the study was a nephroblastoma of the kidney for one male given 3 ppm. Frequently observed nontest material-related neoplasms are listed in Text Table 3. Text Table 3 Incidence of Frequently Observed Neoplasms Pituitary Adenoma Thyroid C-cell Adenoma Thyroid Follicular Cell Adenoma Mammary Adenoma Mammary Fibroadenoma Mammary Carcinoma N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 0 3 30 100 Males Females 65 55 55 65 65 55 55 65 46 28 36 48 48 46 48 45 10 10 10 12 8 13 8 11 03 16 1 024 0001 6 463 3 1 0 0 23 21 16 13 0000 9 9 15 11 Microscopic Findings - Nonneoplastic Findings. The most frequently observed incidental nonneoplastic findings were ventral compression of the brain, pituitary hemorrhage, chronic progressive nephropathy, proteinaceous casts of the kidneys, ultimobranchial cysts of the thyroid, pelvic mineralization of the kidneys, alveolar macrophages in the lungs, ventricular dilatation of the brain, and hemorrhage of the adrenal cortex. Frequent nontest material-related microscopic findings for males were chronic active inflammation of the prostate, chronic inflammation of the liver, vacuolation of the adrenal cortex, degeneration and fibrosis of the heart, vascular mineralization of the lungs, acinar atrophy of the pancreas, and erosion, fibrosis, and acute inflammation of foot pads. Other frequently observed incidental microscopic findings for females were mammary galactoceles, angiectasis of the adrenal cortex, ovarian cysts, uterine dilatation, thymic cysts, and bile duct hyperplasia of the liver. 48 Covance 6329-212 3M T-6316.1 Week 4 Sacrifice Organ Weights. Terminal body weights were lower for males given 300 ppm. Absolute and relative liver weights were higher for males and females given 100 or 300 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. There were no gross findings noted at necropsy. Week 8 Sacrifice Organ Weights. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 300 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. The only test material-related findings at necropsy were large livers for five males and two females given 300 ppm and mottling of the liver for one female given 300 ppm. There were no other test material-related gross findings noted at necropsy. Microscopic Findings. Test material-related findings were limited to the liver. The test material-related hepatic findings were hepatocellular hypertrophy, hepatocellular necrosis, and hemorrhage. The incidence and severity of the hepatic lesions are listed in Text Table 4. 49 Covance 6329-212 3M T-6316.1 Text Table 4 Incidence and Severity of Test Material-Related Microscopic Findings Dose Level Number examined Liver Hypertrophy Moderate Moderately-severe Necrosis Minimal Moderate Hemorrhage N-Ethyl Perfluorooctanesulfonamido (ppm) 0 300 0 300 Males Females 55 5 5 050 00 0 0 10 02 0 4 1 0 1 Minimal 0 3 0 Moderate 0 0 0 0 1 Week 14 Sacrifice Organ Weights. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 100 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. There were no test material-related gross findings noted at necropsy. Microscopic Findings. The only test material-related finding was hepatocellular hypertrophy for males given 30 or 100 ppm and females given 100 ppm. The incidence and severity of the hepatocellular hypertrophy are listed in Text Table 5. 50 Covance 6329-212 3M T-6316.1 Text Table 5 Incidence and Severity of Hepatocellular Hypertrophy Number examined Liver Hypertrophy Minimal Slight Moderate N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 0 3 30 100 Males Females 55 5 5 555 5 01 1 00 1 00 1 0 000 4 000 1 000 0 2 0 Week 53 Sacrifice Organ Weights. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 100 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. There were no test material-related gross findings noted at necropsy. Microscopic Findings. Hepatocellular hypertrophy and vacuolation of the liver were observed only for animals given 100 ppm. The incidence and severity of hepatocellular hypertrophy and vacuolation are listed in Text Table 6. Other test material-related hepatic findings were hepatocellular necrosis for one male and two females given 100 ppm and bile stasis for one male and two females given 100 ppm. One female given 100 ppm had a hepatocellular adenoma. Hepatocellular adenomas are occasionally observed as an incidental finding in 1-year-old rats. 51 Covance 6329-212 3M T-6316.1 Text Table 6 Incidence and Severity of Hepatocellular Hypertrophy Number Examined Liver Vacuolation Minimal Slight Moderate Hypertrophy Minimal Slight Moderate Moderately Severe N-Ethyl Perfluorooctanesulfonamido (ppm) 0 100 0 100 Males Females 5 10 5 10 03 0 2 03 0 0 03 0 0 00 0 0 03 0 3 02 0 4 05 0 0 Unscheduled Deaths There was no test material-related effect for mortality. The only causes of death observed in three or more animals were chronic progressive nephropathy, hepatocellular adenoma, histiocytic sarcoma, mammary carcinoma, mammary fibroadenoma for females, malignant lymphoma and pituitary adenomas for both sexes. By far the most frequent cause of death was pituitary adenomas. The cause of death was undetermined for 22 males and 5 females. The incidence of the most frequently observed causes of death are listed in Text Table 7. 52 Covance 6329-212 3M T-6316.1 Text Table 7 Incidence of Test Material-Related Cause of Death Unscheduled Sacrifice Animals Examined Chronic progressive nephropathy Hepatocellular adenoma Mammary Carcinoma Mammary Fibroadenoma Histiocytic sarcoma Malignant lymphoma Pituitary adenoma Undetermined N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 0 3 30 100 Males Females 36 28 28 33 37 37 35 30 2 10 2 000 0 02 1 1 00 1 0 000 0 035 3 000 0 540 1 011 1 000 0 110 1 10 0 0 22 15 18 21 27 27 22 17 745 6 000 5 Macroscopic Findings. Diffusely dark livers were observed for four males and three females given 100 ppm. Mottled livers were more frequently observed for females given 100 ppm than for controls. The incidence of mottled livers for females was one control, two females given 3 ppm, and five females given 100 ppm. There were no other test material-related macroscopic findings. Microscopic Findings. For the unscheduled sacrifice animals, cystic degeneration and hepatocellular hypertrophy were increased for males and hepatocellular vacuolation was increased for males and females. Hepatocellular adenomas were minimally higher for treated animals compared with the controls. Thirty males and 28 females given 100 ppm that were unscheduled deaths were also examined microscopically. The incidence of test material-related microscopic findings for the unscheduled death animals is listed in Text Table 8. 53 Covance 6329-212 3M T-6316.1 T ext Table 8 Incidence o f T est M aterial-R elated M icroscopic Findings U nscheduled D eaths A nim als Exam ined L iv er D egeneration, C ystic H ypertrophy, H epatocellular V acuolation, H epatocellular B-A denom a, H epatocellular a A nim als placed on recovery. N -ethyl Perfluorooctanesulfonam ido (ppm ) 0 3 30 100 100 0 3 30 100 M ales Fem ales 36 28 28 33 30 37 37 35 30 33 2 6 00 0 2 13 8 11 16 12 1 2 6 2 10 0 00 0 00 0 98 6 00 2 0 0 12 2 100a 28 0 0 6 1 Terminal Sacrifice Macroscopic Findings. Enlargement, mottling, and masses of the liver were more frequent for animals given 100 ppm than for the controls. There were no enlarged or diffusely dark livers for the controls at the terminal sacrifice. One male given 3 ppm and three males and one female given 100 ppm had enlarged livers. Diffusely dark livers were present for one male and four females given 100 ppm. The incidence of mottled livers was one control female, two males given 3 ppm, four males and one female given 30 ppm, and five males and three females given 100 ppm. Hepatic masses were noted at necropsy for one control male, one male and one female given 3 ppm, and three males and three females given 100 ppm. Microscopic Findings. Test material-related hepatic findings at the terminal sacrifice for animals given 100 ppm were cystic degeneration, hepatocellular hypertrophy, and vacuolation. There was only one hepatocellular carcinoma in the study and it was present for a terminal sacrifice female given 100 ppm. The incidence of test material-related microscopic findings at the terminal sacrifice is listed in Text Table 9. 54 Covance 6329-212 3MT-6316.1 Text Table 9 Incidence of Test Material-Related Microscopic Findings Terminal Sacrifice Animals Examined Liver Degeneration, Cystic Hypertrophy, Hepatocellular Vacuolation, Hepatocellular B-Adenoma, Hepatocellular M-Carcinoma, Hepatocellular N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 0 3 30 100 Males Females 14 22 22 17 13 13 15 19 3 5 12 12 00 05 4 8 9 13 13 3 4 00 00 0201 0 0 05 4 6 2 12 0 3 24 0001 Recovery Sacrifice Macroscopic Findings. There were no test material-related macroscopic findings at the recovery sacrifice. Microscopic Findings. The microscopic findings for the recovery sacrifice animals given 100 ppm were similar to the controls. However, 2 of the 12 females had hepatocellular adenomas. Robert L. Hall, DVM, PhD Diplomate, ACVP (Clinical Pathology) ` . / i> ' 1 ^J / .. James L. Carter, DVM, PhD Diplomate, ACVP Date 55 Covance 6329-212 56 Colorado Pathology Services, Inc. Covance 6329-212 3MT-6316.1 Project Number: P-1921 Introduction A peer review of the histopathology portion of the study entitled: 104-Week Dietary Carcinogenicity StudywithNarrowRange (98.1%)N-Ethyl PerfluorooctanesulfonamidoEthanol in Rats" was conducted at Covance, Madison, Wisconsin, By Donald N. Kitchen, D.V.M., PhD of Colorado Pathology Services, Inc., Collbran, Colorado. The tissues had been previously examined and the data tabulated by pathologist, Dr. James L. Carter, at Covance. Experimental Design A summary of the experimental design for the study is presented below: Group 1 (Control) b 2 (Low) 3 (Mid) 4 (Mid-High) 5 (High)c 6 (Mid-High Recovery) 7 (High Recovery)c Study 6329-212 No. of Animals Male Female 70 70 60 60 60 60 70 70 70 70 40 40 40 40 Dietary Levels a (ppm NEtFOSE) 0 3 30 100 300 100 300 T-6316 is 98>1% n-ethyl perfluorooctanesulfonamido ethanol (NetFose); dose levels are expressed as ppm ofNEtFOSE. The control animals received the basal diet only. Five animals/sex in Group 5 were sacrificed during Week 8; the remaining animals in Groups 5 and 7 were sacrificed-and discarded during Week 8. 57 Colorado Pathology Services, Inc. Covance 6329-212 3M T-6316.1 Project Number: P-1921 Group 8 (Control) 9 (Low dose) Study 6329-228 No. of Animals Male Female 70 70 70 70 Dietary Levels (ppm NEtFOSE) 0 1.0 Methods The peer review as conducted consisted of three tasks. The first task was the complete review ofall slides and confirmation ofall diagnoses for 10% ofthe rats in the control and high dose (Groups 1 and 4) including the recovery (Group 6) of study 6329-212. The animals for complete review were selected by Covance and included the following: Males Control Group 4 90714 90904 90724 90914 90734 90124 90744 90934 90754 90944 90764 90954 90774 90964 Main Study -- Females Control Group 4 91124 91314 91134 91324 91144 91334 91154 91344 91164 91354 91174 91364 91184 91374 58 Colorado Pathology Services, Inc. Covance 6329-212 3MT-6316.1 Project Number: P-1921 The second task consisted ofvalidation ofall neoplasms diagnosed by the study pathologist to determine if an acceptable diagnosis had been made. Thethird task consisted ofa review oflivers fromall animalsand thyroid glands fromallmale animals, suspected target tissues. Tasks two and three were performed on both studies, ie. 6329-212 and 6329-228. Results All the materials necessaryforthis reviewwere readilyavailableandwell organized. Relevant in-life data and necropsy data were available to the reviewing pathologist. All slides required to be examinedbythe reviewing pathologist, were present andwere consideredto be ofgood quality. The quality ofthe histotechnique and the hematoxylin and eosin (H&E) staining were also considered to be good. No tissue artifacts were present that interfered with the slide evaluation and the stain providedgood balancebetween nuclear and cytoplasmic staining. All slideswere adequatelylabeled. Dr. James L. Carter the study pathologist, and Marcia J. Holland, histology supervisor, were especially helpful in providing the reviewing pathologist with materials and information needed to complete the peer review. The differences noted during this review were examined and discussed by the study pathologist, the reviewing pathologist and the 3Mpathologist (Dr. Marvin Case) utilizing a multiple headed microscope. All differences noted duringthis reviewwere resolved with complete agreement concerning the final diagnoses. 4 59 Colorado Pathology Services, Inc. Covance 6329-212 3M T-6316.1 Project Number: P-1921 Summary The excellent organization of all materials prior to the arrival of the reviewing pathologist greatly aided and simplified this peer review. The slides were well identified, organized by task and ofexcellent quality. All differences noted duringthis reviewwere resolved with complete agreement concerning the final diagnoses. a . Donald N. Kitchen, D.V.M., Ph.D. Veterinary Pathologist Diplomate: ACVP Date 5 60 Covance 6329-212 3M T-6316.1 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC AND NEOPLASTIC LESIONS Methods Survival. Evaluations of trend and heterogeneity of survival data were performed using the Cox-Tarone binary regression method and the National Cancer Institute (NCI) Life Table Package (Thomas, Breslow, and Gart, 1977). Week 105 was treated as the end of the study in the NCI package for both sexes. Animals that died accidentally and those that were sacrificed at scheduled intervals were censored in the analysis. Continuity corrected one-sided tail probabilities for trend and two-sided tail probabilities for group comparisons were evaluated at the 5.0% significance level as reported in Text Tables 1 and 2. Kaplan-Meier product limit survival curves are presented in Text Figures 1 and 2 for males and females, respectively. Nonneoplastic Lesions. Nonneoplastic lesions were chosen for statistical analyses if the incidence rate in at least one treated group (Groups 2 through 4) was increased or decreased by at least two occurrences over the control group (Group 1). The high recovery group (Group 6) was selected based on the incidence rate (%) if it was increased or decreased by at least two as compared to the control or high-dose group. When there was only one incidence in the high recovery and none in the other groups, that case was excluded from statistical evaluation. These were analyzed by the Cochran-Armitage test for trend and the Fisher-Irwin exact test for control versus treatment comparisons (Thakur, Berry, and Mielke, 1985). One-sided tail probabilities for trend and group comparisons were evaluated at the 5.0% significance level and are reported in Text Tables 3 and 4 for males and females, respectively. Neoplastic Lesions. Neoplastic lesions were chosen for statistical analyses if the incidence rate in at least one treated group was increased or decreased by at least two occurrences over the control group. The incidental tumors (i.e., tumors that were not assigned to be the cause of death of the animals by the study pathologist) were analyzed by logistic regression of tumor prevalence (Dinse and Lagakos, 1983). The logistic regression model included linear and quadratic terms in age (week of death), but the quadratic term was eliminated if there was a lack of convergence. The fatal and palpable (superficial) tumors were analyzed by Cox-Tarone binary regression method, as in the case of survival, using the death time or the first palpation time (as applicable) as the tumor onset time. In the case of any particular tumor type where the study pathologist assigned the tumor in question as being the cause of death of a subset of the animals and the rest of the 61 Covance 6329-212 3M T-6316.1 animals were assumed to be dead of other competing risks, IARC-type (Peto, et al., 1980) cause of death analysis was performed. Specifically, the subset of the tumors, which were assigned to be the cause of death by the examining pathologist, was analyzed by Cox-Tarone logistic regression under life table techniques. The subset, which was considered incidental by the pathologist, was analyzed by logistic regression of tumor prevalences. For the cases of tumor types where cause of death was undetermined, these were treated as incidental for statistical evaluation. The score statistics and their respective variances from the above tests were then used to compute the combined evidence as described by Gart, et al., (1986). In the case of only one tumor belonging to one of the two categories (fatal and incidental) in a test, it was combined with the other category for the purpose of statistical analyses. In addition, in cases where there was lack of convergence for the asymptotic test of the logistic regression method, the exact probability of the test was obtained by using LogXact-Turbo (1993) and combined with the probability of the Cox-Tarone test, if necessary. Common and rare tumors were evaluated at the 1.0% and 5.0% significance levels, respectively, for trend. The benign and malignant neoplastic incidences were evaluated separately, but were combined where appropriate. The criteria for combination were based on the work of McConnell et al. (1986). The incidences of multiple-organ findings such as hemangioma, hemangiosarcoma, lipoma, liposarcoma, fibroma, fibrosarcoma, leiomyoma, leiomyosarcoma, endometrial stromal polyp, and endometrial stromal sarcoma were counted by animal, not by tissue type. They were evaluated statistically if they met the selection criterion for the analysis. The statistical results for these cases may be biased because not all the animals were examined for every tissue. Pairwise one-sided group comparisons were evaluated at the 5.0% significance level. One-sided tail probabilities for trend analysis and group comparisons are shown in Text Tables 5 and 6 for males and females, respectively. Groups 5 (High-dose) and 7 (High recovery) were excluded from all the analyses except the survival graphs (Text Figures 1 and 2) due to early sacrifices at Week 8. In the nonneoplastic analyses, animals sacrificed early (Week 4) in Groups 1 through 4 (Control, Low, Mid, and Mid-High, respectively) were excluded from the denominators. In the statistical evaluations of the neoplastic lesions, animals sacrificed early (during or before Week 14) in Groups 1 through 4 were discounted from the number of animals at risk. Further, in the neoplastic analyses, in the cases where the low, mid, and mid-high recovery groups did not have a complete histopathology examination, they were excluded from statistical analyses. 62 Covance 6329-212 3M T-6316.1 In the text tables, the p-values under Group 1 (Control) are for trend analysis and the p-values under Groups 2 (Low), 3 (Mid), 4 (Mid-High), and 6 (Mid-High recovery) are for the comparison of that group versus the control group. Group 6 was also compared with Group 4, and the p-values for those comparisons are indicated in the text tables. Trend analysis includes Groups 1, 2, 3, and 4 only. Ordinal dose levels 0, 1, 2, and 3 were used in all analyses for Groups 1, 2, 3, and 4, respectively. Continuity correction was done for all asymptotic tests. The following notations are used throughout this report to designate direction and significance of effects. += -= *= ** = E= NA = Effect in the increased direction Effect in the decreased direction Significant at p < 0.05 Significant at p < 0.01 Exact permutation test Not analyzed Results and Discussion Survival. As indicated in Text Tables 1 and 2, no significant trends or group comparisons in mortality were observed in males and females. Nonneoplastic Lesions. The liver in both sexes has been identified as the only organ that is appropriate to perform statistical analysis for nonneoplastic lesions. As indicated in Text Table 3, there were significant positive trends in the incidences of hepatocellular vacuolation and hepatocellular centrilobular hypertrophy (p = 0.00003 and 0.00000, respectively) for males. These cases were associated with significant increases in the mid-high dose group (p = 0.00009 and 0.00000, respectively), when compared with the control. However, the mid-high recovery group animals in these cases exhibited significantly decreased incidences over the mid-high dose group (p = 0.00107 and 0.00035, for both cases) implying that the effect may be transient (reversible) in nature. A significant positive trend was also observed for cystic degeneration (p = 0.00183) in association with significantly increased incidences in both the mid- and mid-high dose males over the control (p = 0.01634 and 0.00589, respectively). There was no such effect in the females for this parameter. Unless this is a sex specific effect, it can be considered to be a chance variation. Further, significantly increased incidences 63 Covance 6329-212 3M T-6316.1 were observed in the mid-high recovery males, when compared with both control and mid-high dose males for hemorrhage (p = 0.03442 and 0.00462, respectively) and the low-dose group for telangiectasis as compared to the control (p = 0.04854). However, no significant trends were found in these cases, which diminishes their importance. As indicated in Text Table 4, there were significant positive trends in the incidences of hepatocellular vacuolation, hepatocellular centrilobular hypertrophy, and bile duct hyperplasia (p = 0.02018, 0.00001, and 0.00463, respectively) for females. These cases were associated with significant increases in the mid-high-dose group (p = 0.01050, 0.00025, and 0.01750, respectively), when compared with the control. However, the mid-high recovery group animals have exhibited significantly decreased incidences over the mid-high-dose group (p = 0.02638, 0.00066, and 0.00782, respectively), which implies their transient nature. Marginally significant increases over the control were also revealed in the incidences of low- and mid-high-dose groups for chronic inflammation (p = 0.03410 and 0.02681, respectively). However, no significant trend was found in this case. Neoplastic Lesions. As indicated in Text Table 5, significant positive trends were noted only in the incidences of thyroid follicular cell adenoma and its combined adenoma and carcinoma (p = 0.0171 and 0.0155, respectively) for males. These cases were associated with significant increases in the mid-high-dose group (p = 0.0107, for both cases) over the control. However, thyroid follicular cell carcinoma did not show any significant effect. Also, the trends mentioned earlier are nonmonotonic. Follicular cell adenoma in the thyroid of the Sprague Dawley rat is a common tumor (>1% background) for which the FDA uses a 1.0% significance level for trend evaluation. Therefore, these trends are not deemed to be biologically significant. There were no other significant neoplastic findings in the males of this study. As indicated in Text Table 6, significant positive trends were observed only in the incidences of hepatocellular adenoma and combined adenoma and carcinoma (p = 0.0050 and 0.0022, respectively) for females. These cases were associated with significant increases in the mid-high-dose and mid-high recovery groups (p = 0.0120 and 0.0498, respectively for adenoma and p = 0.0069 and 0.0498, respectively, for adenoma/carcinoma), as compared with the control. However, hepatocellular carcinoma did not show a significant effect. There were significant negative trends in the incidences of mammary fibroadenoma; adenoma and fibroadenoma combined; and the combined adenoma, fibroadenoma, and carcinoma incidences (p = 0.0033, 0.0051, and 0.0122, 64 Covance 6329-212 3M T-6316.1 respectively). All these cases were associated with significantly lower incidences in the respective mid-high-dose group (p = 0.0094, 0.0113, and 0.0181, respectively). Furthermore, a marginally decreased incidence over the control was noted for the mammary combined fibroadenoma, adenoma, and carcinoma incidences (p = 0.0403). No significant trend or group comparisons were found in the incidences ofmammary carcinoma. Significant negative trends were also noted in the incidences of pituitary adenoma and combined adenoma and carcinoma (p = 0.0291, for both cases). But none of the pairwise comparisons were significant in these cases. There was no significant trend or group effect in the pituitary carcinoma. There were no other significant neoplastic findings in females. References Dinse, G.E. and Lagakos, S.W., "Regression analysis of tumor prevalence data," Journal o f R o ya l S tatistical Society Series C (A pplied Statistics), 32:236-248 (1983). Gart, J.J., Krewski, D., Lee, P.N., Tarone, R.E., and Wahrendorf, J., S tatistical M ethods in C ancer Research: The D esign a n d A n alysis o f Long-Term A n im al E xperim ents, IARC Scientific Publications, Vol. 3, No. 79, Oxford University Press, New York (1986). LogXact-Turbo, Cytel Software Corporation, Version 1.1, Cambridge, Massachusetts (1993). McConnell, E.E., Solleveld, H.A., Swenberg, J.A., and Boorman, G.A., "Guidelines for combining neoplasms for evaluation of rodent carcinogenicity studies," Journal o f N ation al Cancer Institute, 76:283-289(1986). Peto, R., Pike, M.C., Day, N.E., Gray, R.G., Lee, P.N., Parish, S., Peto, J., Richards, S., and Wahrendorf, J., "G uidelines f o r simple, sensitive significance tests f o r carcinogenic effects in long-term anim al experiments, in Long-term an d Short-term Screening A ssays f o r Carcinogens: A C ritical A ppraisal," Lyon: International Agency for Cancer Research (IARC), 311-426 (1980). Thakur, A.K., Berry, K.J., and Mielke, P.W., Jr., "A FORTRAN program fortesting trend and homogeneity in proportions," Com puter P rogram s in Biom edicine, 19:229-233 (1985). Thomas, D.G., Breslow, N., and Gart, J. J., "Trend and homogeneity analyses of proportions and life table data," Com puters a n d B iom edical Research, 10:373-381 (1977). 65 Covance 6329-212 3M T-6316.1 f\ A^vywJ Arjun Roy, MA Staff Scientist and Biostatistician Department of Safety Assessment and Biostatistics | ^ 111 10 J Date Ajit K. Thakur, PhD Principal Scientist and Biostatistician Department of Safety Assessment and Biostatistics 66 Covance 6329-212 3M T-6316.1 Text Table 1 Results of Statistical Analyses of Survival Data for Male Rats Group Unadjusted Mortality Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 1 Control 36/70 (.514) .718 .064 2 Low 28/60 (.467) .559 .070 3 Mid 27/60 (.450) .551 .071 4 Mid-High 33/70 (.471) .657 .067 6 Mid-High Recovery 30/40 (.750) .750 .068 Groups 1vs 2-4 Trend p (one-sided) Groups 1vs 2 p (two-sided) Groups 1vs 3 p (two-sided) Groups 1vs 4 p (two-sided) Groups 1vs 6 p (two-sided) Groups 4 vs 6 p (two-sided) Cox-Tarone Test .2438 .2381 .1736 .4952 .7216 + .2828 + T ext Table 2 R esults o f Statistical A nalyses o f Survival D ata for Fem ale R ats Group Unadjusted Mortality Adjusted Mortality: Kaplan-Meier Estimate (Final) Standard Error 1 Control 37/70 (.529) .740 .062 2 Low 37/60 (.617) .740 .062 3 Mid 35/60 (.583) .700 .065 4 Mid-High 30/70 (.429) .611 .070 6 Mid-High Recovery 28/40 (.700) .700 .072 Groups 1vs 2-4 Trend p (one-sided) Groups 1vs 2 p (two-sided) Groups 1vs 3 p (two-sided) Groups 1vs 4 p (two-sided) Groups 1vs 6 p (two-sided) Groups 4 vs 6 p (two-sided) Cox-Tarone Test .0821 .9118.8451 .2020 .7645 .4088 + 67 Covance 6329-212 3M T-6316.1 Text Table 3 Results of Statistical Analyses of Nonneoplastic Lesions in the Males 123 Group Control Low Mid LIVER NECROSIS, HEPATOCELLULAR 1/65 3/55 4/55 p-values .33770 + .24911 + .13458+ 46 Mid-High Mid-High Recovery Groups 4 vs 6 2/65 4/40 NA .06833 + .14708+ LIVER HEMORRHAGE 3/65 3/55 p-values .24261 - NA 3/55 1/65 NA .30956 - 7/40 .03442 +* .00462 +** LIVER INFLAMMATION, CHRONIC 18/65 20/55 23/55 p-values .31850 + .20587+ .07604+ 20/65 .42364+ 9/40 .36217 - .24493 - LIVER VACUOLATION, HEPATOCELLULAR 17/65 18/55 23/55 39/65 p-values .00003 +** NA .05271 + .00009 +** 11/40 .52672+ .00107 -** LIVER CENTRILOBULAR HYPERTROPHY, HEPATOCELLULAR 0/65 1/55 3/55 22/65 2/40 p-values .00000 +** NA .09342+ .00000 +** .14286+ .00035 -** LIVER HYPERPLASIA, BILE DUCT 9/65 9/55 p-values .37867+ NA 8/55 NA 11/65 .40426+ 5/40 .54603 - .37555 - LIVER DEGENERATION, CYSTIC 6/65 8/55 14/55 p-values .00183 +** .26759+ .01634+* 18/65 .00589 +** 7/40 .17208+ .17015 - LIVER FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC 8/65 12/55 11/55 6/65 2/40 p-values .32152 - .12579+ .18421 + .38915 - .18700- .34905 - LIVER TELANGIECTASIS 2/65 7/55 p-values .12470- .04854 +* 3/55 NA 0/65 .24806 - 0/40 .38095 - NA LIVER CYST 1/65 p-values .30780+ 0/55 NA 0/55 NA 2/65 NA 0/40 NA .38095 - LIVER LIPIDOSIS, TENSION 0/65 2/55 p-values .21735+ .20798+ 1/55 NA 2/65 .24806+ 0/40 NA .38095- LIVER FIBROSIS 0/65 0/55 1/55 p-values NA NA NA 0/65 NA 2/40 .14286 + .14286 + 68 Covance 6329-212 3M T-6316.1 T ext Table 4 R esults o f Statistical A nalyses o f N onneoplastic Lesions in the Fem ales 1 23 Group Control Low Mid LIVER NECROSIS, HEPATOCELLULAR 5/65 4/55 4/55 p-values .41816 + NA NA LIVER HEMORRHAGE 4/65 2/55 p-values .10451 - .42223 - 4/55 NA 46 Mid-High Mid-High Recovery Groups 4 vs 6 6/65 3/40 NA .65095 - .53010 - 0/65 .05960 - 3/40 NA .05270+ LIVER HEMATOPOIESIS, EXTRAMEDULLARY 1/65 1/55 0/55 3/65 p-values .21735 + NA NA .30956+ 1/40 NA .50714- LIVER INFLAMMATION, CHRONIC 9/65 16/55 10/55 p-values .06223 + .03410+* NA 19/65 .02681 +* 8/40 .28521 + .20687 - LIVER VACUOLATION, HEPATOCELLULAR 13/65 14/55 8/55 26/65 p-values .02018+* NA .29527 - .01050 +* 8/40 .59441 .02638 -* LIVER CENTRILOBULAR HYPERTROPHY, HEPATOCELLULAR 1/65 0/55 0/55 14/65 0/40 p-values .00001 +** NA NA 00025 +** .61905 - .00066 -** LIVER HYPERPLASIA, BILE DUCT 4/65 3/55 6/55 p-values .00463 +** NA .27110+ 13/65 .01750 +* 1/40 .3660 - .00782 -** LIVER DEGENERATION, CYSTIC 0/65 2/55 p-values .25667+ .20798+ 0/55 NA 2/65 .24806+ 1/40 NA NA LIVER STASIS, BILE 0/65 p-values .06250+ 0/55 NA 0/55 2/65 NA .24806+ 0/40 NA .38095 - LIVER FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC 0/65 2/55 2/55 1/65 p-values .34805 + .20798+ .20798+ NA 0/40 NA NA LIVER FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC 3/65 7/55 2/55 7/65 p-values .26768 + .10200 + NA .16203 + 2/40 NA .25853 - 69 Covance 6329-212 3M T-6316.1 Text Table 4 (Continued) Results of Statistical Analyses of Nonneoplastic Lesions in the Females 12 Group Control Low LIVER TELANGIECTASIS 3/65 6/55 p-values .45263 - .16964 + 3 Mid 1/55 .37488 - 4 Mid-High 4/65 NA 6 Mid-High Recovery 5/40 .13634+ Groups 4 vs 6 .21870+ LIVER LIPIDOSIS, TENSION 0/65 1/55 p-values NA NA 0/55 NA 0/65 NA 2/40 .14286+ .14286+ LIVER FIBROSIS 0/65 p-values .41376 - 3/55 .09342 + 1/55 NA 0/65 NA 1/40 NA NA 70 Covance 6329-212 3M T-6316.1 Text Table 5 Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 Group Control Liver - Hepatocellular, Adenoma Fatal Incidence 0 Incidental Incidence 2 Total Incidence Rate 2/55 2 Low 2 2 4/50 34 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 11 14 2/50 5/60 0 0 0/40 One-sided p-values .2514 + .3324 + NA .1883 + .3149 - .0854 - Pancreas - Islet Cell, Adenoma Fatal Incidence 0 Incidental Incidence 4 Total Incidence Rate 4/55 0 7 7/50 0 2 2/50 0 6 6/60 0 2 2/40 One-sided p-values .4817 - .2869 + .2575 - .3040 + .4753 - .3161 - Pancreas - Islet Cell, Carcinoma Fatal Incidence 00 0 0 Incidental Incidence 0 0 1 0 Total Incidence Rate 0/55 0/50 1/50 0/60 Note: Incidences across groups do not meet the selection criterion. 0 0 0/40 71 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 12 Group Control Low Pancreas - Islet Cell, Adenoma/Carcinoma Fatal Incidence 00 Incidental Incidence 4 7 Total Incidence Rate 4/55 7/50 34 6 Mid Mid-High Mid-High Recovery 00 36 3/50 6/60 0 2 2/40 One-sided p-values .4678 + .2869 + .4072 - .3040 + .4753 - Adrenal Medulla - Pheochromocytoma, Benign Fatal Incidence 00 Incidental Incidence 9 6 Total Incidence Rate 9/55 6/29 0 4 4/31 0 5 5/60 0 1 1/31 One-sided p-values NA NA NA .2227 - NA Testis - Interstitial Cell Tumor, Benign Fatal Incidence 00 Incidental Incidence 3 1 Total Incidence Rate 3/55 1/50 0 3 3/50 0 1 1/60 0 0 0/40 One-sided p-values .2566 - .2620 - NA .1646 - .1779 - Covance 6329-212 3M T-6316.1 Groups 4 vs 6 .3161 - NA NA 72 Covance 6329-212 3M T-6316.1 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 Group Control Thyroid - C-Cell, Adenoma Fatal Incidence 0 Incidental Incidence 10 Total Incidence Rate 10/55 2 Low 0 10 10/50 3 Mid 0 10 10/50 46 Mid-High Mid-High Recovery Groups 4 vs 6 0 12 12/60 0 5 5/40 One-sided p-values .3344+ NA NA .3320 + .3100 - .1843 - Thyroid - C-Cell, Carcinoma Fatal Incidence 000 0 Incidental Incidence 0 0 1 0 Total Incidence Rate 0/55 0/50 1/50 0/60 Note: Incidences across groups do not meet the selection criterion. 0 1 1/40 Thyroid - C-Cell, Adenoma/Carcinoma Fatal Incidence 00 Incidental Incidence 10 10 Total Incidence Rate 10/55 10/50 0 11 11/50 0 12 12/60 0 6 6/40 One-sided p-values .3124 + NA NA .3320 + .3100 - .1843 - 73 Covance 6329-212 3M T-6316.1 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats 1 Group Control Thyroid - Follicular Cell, Adenoma Fatal Incidence 0 Incidental Incidence 0 2 Low 0 3 34 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 00 16 0 1 One-sided p-values .0171 +* .1662 + NA .0107 +* NA .1204 - Thyroid - Follicular Cell, Carcinoma Fatal Incidence 00 0 0 Incidental Incidence 0 0 1 0 Total Incidence Rate 0/55 0/50 1/50 0/60 Note: Incidences across groups do not meet the selection criterion. 0 1 1/40 Thyroid - Follicular Cell, Adenoma/Carcinoma Fatal Incidence 00 Incidental Incidence 0 3 Total Incidence Rate 0/55 3/50 0 2 2/50 0 6 6/60 0 2 2/40 One-sided p-values .0155 +* .1662 + .3667 +(E) .0107 +* .1713 + .2303 - 74 Covance 6329-212 3M T-6316.1 Text Table 5 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Male Rats Group Pituitary - Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 1 Control 23 23 46/55 2 Low 15 13 28/33 3 Mid 17 19 36/38 46 Mid-High Mid-High Recovery Groups 4 vs 6 22 26 48/60 20 11 31/36 One-sided p-values NA NA NA .4108 - .1616 + .1037+ Skin - Papilloma, Squamous Cell Total Incidence Rate 0/55 3/35 0/33 2/60 3/32 One-sided p-values NA NA NA .2509 + NA NA Multiple Organs - Fibroma Total Incidence Rate 2/55 1/35 1/33 0/60 0/32 One-sided p-values NA NA NA .1957 -(E) NA NA Multiple Organs - Fibrosarcoma Total Incidence Rate 1/55 0/29 0/28 0/60 Note: Incidences across groups do not meet the selection criterion. 0/30 Multiple Organs - Fibroma/Fibrosarcoma Total Incidence Rate 3/55 1/35 1/33 0/60 0/32 One-sided p-values NA NA NA .0946 - NA 75 Covance 6329-212 3M T-6316.1 Text Table 6 Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 Group Control Liver - Hepatocellular, Adenoma Fatal Incidence 0 Incidental Incidence 0 Total Incidence Rate 0/55 2 Low 0 1 1/50 34 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 10 26 3/50 6/60 0 3 3/40 One-sided p-values .0050 +** NA .0796 + .0120 +* .0498 +* .4879 - Liver - Hepatocellular, Carcinoma Fatal Incidence 0 000 Incidental Incidence 0 001 Total Incidence Rate 0/55 0/50 0/50 1/60 Note: Incidences across groups do not meet the selection criterion. 0 0 0/40 Liver - Hepatocellular, Adenoma/Carcinoma Fatal Incidence 0 0 10 Incidental Incidence 0 127 Total Incidence Rate 0/55 1/50 3/50 7/60 0 3 3/40 One-sided p-values .0022 +** NA .0796 + .0069 +** .0498 +* .3805 - 76 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 Group Control Pancreas - Islet Cell, Adenoma Fatal Incidence 0 Incidental Incidence 1 Total Incidence Rate 1/55 2 Low 0 4 4/50 34 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 00 11 1/50 1/60 0 1 1/40 One-sided p-values .2252 -(E) .1609 +(E) NA NA NA NA Adrenal Medulla - Pheochromocytoma, Benign Fatal Incidence 0 Incidental Incidence 0 Total Incidence Rate 0/55 2/40 1/39 0 2 2/60 1/30 One-sided p-values NA NA NA .3448 +(E) NA NA Thymus - Thymic, Carcinoma Fatal Incidence 0 001 Incidental Incidence 0 0 0 1 Total Incidence Rate 0/55 0/36 0/35 2/59 0 0 0/25 One-sided p-values NA NA NA .1029 + NA NA 77 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 Group Control Thyroid - C-Cell, Adenoma Fatal Incidence 0 Incidental Incidence 8 Total Incidence Rate 8/55 2 Low 0 13 13/50 34 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 00 8 11 8/50 11/60 0 8 8/40 One-sided p-values .4920 + .1323 + NA .3258 + NA .4676 + Thyroid - C-Cell, Carcinoma Fatal Incidence 10 0 0 Incidental Incidence 0 0 0 0 Total Incidence Rate 1/55 0/50 0/50 0/60 Note: Incidences across groups do not meet the selection criterion. 0 0 0/40 Thyroid - C-Cell, Adenoma/Carcinoma Fatal Incidence 10 Incidental Incidence 8 13 Total Incidence Rate 9/55 13/50 0 8 8/50 0 11 11/60 0 8 8/40 One-sided p-values .4866 - .1990 + NA .4095 + NA .4676 + 78 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 Group Control Thyroid - Follicular Cell, Adenoma Fatal Incidence 0 Incidental Incidence 1 Total Incidence Rate 1/55 2 Low 0 0 0/50 34 6 Mid Mid-High Mid-High Recovery Groups 4 vs 6 00 24 2/50 4/60 0 1 1/40 One-sided p-values .0617 + NA NA .1139+ NA .2909 - Pituitary - Adenoma Fatal Incidence Incidental Incidence Total Incidence Rate 26 22 48/55 28 18 46/48 23 25 48/49 17 28 45/59 16 18 34/38 One-sided p-values .0291 -* .3193 + NA .0962 - .3276 - .1149 + Pituitary - Carcinoma Fatal Incidence 00 0 0 Incidental Incidence 0 0 0 0 Total Incidence Rate 0/55 0/48 0/49 0/59 Note: Incidences across groups doe not meet the selection criterion 1 0 1/38 79 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 Group Control Pituitary - Adenoma/Carcinoma Fatal Incidence 26 Incidental Incidence 22 Total Incidence Rate 48/55 2 Low 28 18 46/48 3 Mid 23 25 48/49 46 Mid-High Mid-High Recovery Groups 4 vs 6 17 28 45/59 17 18 35/38 One-sided p-values .0291 -* .3193 + NA .0962 - .4362 - .0644 + Mammary - Adenoma Total Incidence Rate 6/54 4/50 6/50 3/60 4/40 One-sided p-values .1522 - .3187 - NA .1492 - .4486 - .3332 + Mammary - Fibroadenoma Total Incidence Rate 23/54 21/50 16/50 13/60 12/40 One-sided p-values .0033 -** .4531 - .0727 - .0094 -** .0525 - .3506 + Mammary - Adenoma/Fibroadenoma Total Incidence Rate 26/54 24/50 21/50 16/60 15/40 One-sided p-values .0051 -** .3923 - .1200 - .0113 -* .0801 - .2876 + 80 Covance 6329-212 3M T-6316.1 Text Table 6 (Continued) Results of Statistical Analyses of Neoplastic Lesions - Female Rats 1 23 4 6 Group Control Low Mid Mid-High Mid-High Recovery Groups 4 vs 6 Mammary - Carcinoma Total Incidence Rate 9/54 9/50 15/50 11/60 6/40 One-sided p-values .3277 + NA .1399 + .4323 + .4108 - .3616 - Mammary - Adenoma/Fibroadenoma/Carcinoma Total Incidence Rate 32/54 29/50 28/50 23/60 18/40 One-sided p-values .0122 -* .4055 - .2364 - .0181 -* .0403 -* .4781 + Multiple Organs - Leiomyoma Total Incidence Rate 3/55 0/41 0/39 1/60 1/32 One-sided p-values NA NA NA .1710 -(E) NA NA 81 Covance 6329-212 3M T-6316.1 TEXT FIGURE 1 KAPLAN-MEIER PRODUCT LIMIT ESTIMATE CURVES OF SURVIVAL: MALE RATS 0 15 30 45 60 75 90 105 0 +******* ***2 22 22 22 22 22 22 22 22 22 22 22 22 2 + 6 ** **** ** ** ** ** ** ** ** ** ** *3 22 2 2 2 2 2 + 45 1 1 1 1 1 1 ** ** ** ** * * * 0 * 2 2 2 2 + 555 55 55 55 55 55 55 55 55 55 5 1*3 *33 22 + 55 55 55 1 003 2 9+ P 5 55 55** *0*3 3* ****1*2 R 50***2 O P+ 555**22 5 5 *** 0 .8 + 0***5 R+ T+ 0*0**5 3 3 *** 1+ 0 N .7+ + S+ ***5 *** ****3 **0 * 2***2222 U+ 55***1* R+ V .6 + 5 0*3* 55 03**2 1+ 5 00**2 V+ I+ 5 0**22 5 0 *** N+ 555 0 3*2 G .5+ 55 0 ***22 + 55*0 1* 2 + 50 * 2 + 500 *1** + 5 00 33 .4 + 555* 33 + *0 3 + 500 3 + 55* 33 +* .3+ ** + **0 +5 + 55 + 2 + 0 = Group 1 (Control) + 1 = Gro u p 2 (Low) + 2 = Gro u p 3 (Mid) + 3 = Group 4 (Mid-High) + 4 = Gro u p 5 (High) 1 + 5 = Group 6 (Mid-High Recovery) + 6 = Group 7 (High Recovery) + * = More Than One Data Point + + .0+ 0 15 30 45 60 75 90 105 TIME (WEEKS) 82 Covance 6329-212 3M T-6316.1 TEXT FIGURE 2 KAPLAN-MEIER PRODUCT LIMIT ESTIMATE CURVES OF SURVIVAL: FEMALE RATS 0 15 30 45 60 75 90 105 ++ + + + + + + 1 0+*************************************55555 + 3333333333333333********* 55 + **** + 22***1 + 0***** .9+ 00*** P+ ****0 R+ 123*0 O+ 1**** P+ 22*0 O .8+ 2** R+ 25**3 T+ 22*53 I+ 2**** O+ 21* **3 N 7+ 2 * 5*33 + 22***25 3 S+ 0*** 3 U+ 00***3 R+ 0***33 V 6+ 2** 3333 I+ **0 33 V+ 2*0 333 I+ **5 3 N+ *** 3 G 5+ 00** 3 + 01** 333 + 005* 3 + 0***2 333 + **** 3 4+ 05* 3 + 05*2 3 + 00*22 + 0* 222 + **5 2 3+ 0**** + 11* +* + + 2+ 0 = Group 1 (Control) + 1 = Group 2 (Low) + 2 = Group 3 (Mid) + 3 = Group 4 (Mid-High) + 4 = Group 5 (High) 1+ 5 = Group 6 (Mid-High Recovery) + 6 = Group 7 (High Recovery) + * = More Than One Data Point + + 0+ + + ++ + + 0 15 30 45 60 75 TIME (WEEKS) ++ 90 105 83 Covance 6329-212 3M T-6316.1 COMMENTS ON THE DATA Various models of calculators, computers, and computer programs were used to analyze data in this study. Because different models round off or truncate numbers differently, values in some tables (e.g., means, standard deviations, or individual values) may differ slightly from those in other tables, from individually calculated data, or from statistical analysis data. Neither the integrity nor the interpretation of the data was affected by these differences. The units for the dose levels in the inlife summary tables are ppm. The number of animals listed in the heading of the summary tables for clinical and mass observations reflects the number of animals assigned to each group at the start of the study. The summary tables for clinical observations indicates the number of animals for which a condition was observed without regard to the reversibility, number of incidences/animal, or the length of time the condition persisted. Only observations for each animal other than normal are indicated in the summary clinical observations tables. Each animal with observations recorded as "Normal" throughout the study has the comment "No remarkable observations" indicated in the individual clinical observations tables. The specific details for comments in the individual clinical observations tables that are indicated with a "C" can be found at the end of each group for each sex. The values for summary and individual test material consumption are reported with more significant figures than is appropriate for the data due to limitations of the data collection and reporting software. Food consumption values are reported in whole numbers; however, the data collection system carries food consumption values to one place to the right of the decimal for food efficiency and test material consumption calculations. The differences in values generated do not influence the interpretation of the calculation. The comment "SPILLED" in individual food consumption data tables indicates that food consumption was not recorded due to spillage during the interval. The comment "CNTMD" in individual food consumption data tables indicates that food consumption was not recorded due to contamination of the diet with urine or feces in the food container. 84 COMMENTS ON THE DATA (Continued) Covance 6329-212 3M T-6316.1 The comment "NOT TAKEN" in individual food consumption data tables indicates that the animal died before the end of the food consumption interval. Differences in the population size (N) in the summary tables for clinical and anatomic pathology are explained in the individual data tables or the codes sheets or in the Protocol Deviations section. Results of clinical pathology samples collected for animals sacrificed at unscheduled intervals appear in the individual clinical pathology tables for the next scheduled interval. These results are not in the summary tables and are not included in the statistical analyses. Statistical analyses were not done when there were two or fewer values for a parameter. The calculation for individual test material consumption is: Test material consumption = Diet ConcentrationxFood Consumption Food Consumption Intervalx[First Body Weight of Interval+ (Daily Body Weight GainxDay Factor)] where: Daily Body Weight Gain = Last Body Weight of Interval- First Body Weight of Interval Day of Last Body Weight of Interval- Day of First Body Weight of Interval and: Day Factor = Food Consumption Interval (Food Consumption Start Day- Day of First Body Weight of Interval) + 2 and: Food Consumption Interval = Food Consumption End Day- Food Consumption Start Day 85 CODES, ABBREVIATIONS, AND UNITS Covance 6329-212 3M T-6316.1 General Codes and Abbreviations Codes for Clinical Pathology Abbreviations and Units for Clinical Hematology Abbreviations and Units for Clinical Chemistry Abbreviations and Units for Clinical Urinalysis Abbreviations and Units for Urine Chemistry Codes for Anatomic Pathology Note: The following lists of codes, abbreviations, and units are used by Covance. Some, but not necessarily all, of this information may be needed for this report. 86 Covance 6329-212 3M T-6316.1 General Codes and Abbreviations WK N Mean; MEAN CAM SD; S.D.; STAND DEV; STANDARD DEV; sd * -; NA P C UNSCHED DISPATCH Week Number of measurements in a group Arithmetic mean Covariate-adjusted mean Standard deviation Group mean is significantly different from the mean of the control group (Group 1) at p < 0.05 No value; not applicable; not present Present Comment found at the end of each group for each sex Unscheduled Observations transferred from the inlife module of the data collection system to the necropsy module for reference during necropsy. Observations are duplicates of the last inlife observations. Animal Death Codes 1 2 3 4 6 T D M U Interim sacrifice, Week 4 Interim sacrifice, Week 8 Interim sacrifice, Week 14 Interim sacrifice, Week 52 Interim sacrifice, Week 8 Terminal sacrifice Found dead Moribund First postrecovery sacrifice 87 NS QS/QNS NR FS SC SH H SL L SI I NF U DT/DOT DB TJ TE RE EE SE PC PD PI PL PA CO HB PLASMO NO AGG FR UTD NO COAG Covance 6329-212 3M T-6316.1 Codes for Clinical Pathology GENERAL CODES No sample Quantity not sufficient No repeat (sample volume not sufficient for repeat analysis) Fibrin strands Sample clotted Slightly hemolyzed Hemolyzed Slightly lipemic Lipemic Slightly icteric Icteric Animal not fasted Unscheduled/moribund bleed Animal died on test Died during bleeding Technician judgment to repeat test Technical error (instrument or technician error that results in unacceptable data, e.g., unacceptable instrument output, sample spilled, entry of invalid data) Recording error (recorded incorrect data, e.g., wrong number, spelling error, incorrect date) Entry error (incorrect keyboard entry) Sampling error Platelets clumped Platelets decreased Platelets increased Platelets large Platelets appear adequate Color interferes with test Heinz bodies observed Plasmodium No aggregation Fractious Unable to determine No coagulation 88 Covance 6329-212 3M T-6316.1 Codes for Clinical Pathology (Continued) RESULTS NOT INCLUDED IN STATISTICAL ANALYSES Hemolyzed clinical chemistry or coagulation samples Samples from animals at unscheduled intervals Prothrombin times (PT) greater than 50 seconds Activated partial thromboplastin times (PTT) greater than 110 seconds Bleed times (BLETIME) greater than 30 minutes CODES FOR BLOOD CELL MORPHOLOGY The following scale was used to measure the degree of anisocytosis (ANISO), poikilocytosis (POIK), polychromasia (POLY), hypochromasia (HYPO), or basophilic stippling (BASTIP) or the presence of Howell-Jolly bodies (HJBODY), toxic neutrophils (TOXNEUT), or atypical lymphocytes (ATYPLYM). Scale 1 2 3 4 Degree Normal for the species Slight Moderate Marked Not applicable Presence Not present Rare Few Moderate Many URINE APPEARANCE A Pale B Straw C Yellow D Dark yellow Color E Amber F Brown G Red H Green I Black P Blue/green Q Blue R Orange Clarity J Clear K Hazy L Cloudy Miscellaneous M Debris O Feces 89 Codes for Clinical Pathology (Continued) Covance 6329-212 3M T-6316.1 URINE CHEMISTRY MULTISTIX STRIP Urine Glucose + ++ +++ ++++ +++++ Negative 100 mg/dL 250 mg/dL 500 mg/dL 1,000 mg/dL >2,000 mg/dL Urine Ketone + ++ +++ ++++ +++++ Negative 5 mg/dL 15 mg/dL 40 mg/dL 80 mg/dL 160 mg/dL Urine Blood - Negative + Small ++ Moderate +++ Large Urine Urobilinogen + ++ +++ ++++ 0.2 mg/dL 1 mg/dL 2 mg/dL 4 mg/dL 8 mg/dL (1 mg = approximately 1 Ehrlich unit) Urine Bilirubin - Negative + Small ++ Moderate +++ Large URINE SEDIMENT Cells, Crystals, Casts, and Comments A Amorphous urates B Amorphous phosphates C Uric acid D Triple phosphates E Calcium oxalate F Calcium carbonate G Granular casts H Hyaline casts I Cellular casts J Waxy casts K Unknown crystal P Mucous threads Q Sperm R Fecal contamination S Pinworm ova found T Pinworm larvae found U Parasite ova found 0 Not present 1 1 -5 per field 2 6 - 1 0 per field 3 11-20 per field 4 >20 per field Bacteria 0 Not present 1 Few 2 Moderate 3 Many 90 Covance 6329-212 3M T-6316.1 Abbreviations and Units for Clinical Hematology Test R e d b lo o d cell c o u n t H e m o g lo b in H em ato crit M e a n c o rp u scu la r v o lu m e M e a n c o rp u scu la r h em o g lo b in M e a n c o rp u sc u la r h e m o g lo b in c o n c e n tra tio n P la te le t co u n t M e a n p la te le t v o lu m e R e tic u lo c y te c o u n t A b so lu te re tic u lo c y te co u n t H e in z b o d y c o u n t E ry th ro c y te se d im e n tatio n ra te P ro th ro m b in tim e A c tiv a te d p a rtial th ro m b o p la stin tim e T h ro m b in tim e A c tiv a te d c o ag u la tio n tim e F ib rin o g en F ib rin /fib rin o g e n d e g ra d a tio n p ro d u c ts P la te le t a g g reg a tio n C o lla g e n A d e n o sin e d ip h o sp h a te A lp h a 2 -a n tip la sm in B le e d in g tim e M e th e m o g lo b in P la sm a h em o g lo b in M y e lo id /e ry th ro id ratio E stim a te d m y e lo id /e ry th ro id ra tio W h ite b lo o d cell c o u n t D iffe re n tia l b lo o d cell c o u n t S e g m en te d n e u tro p h il c o u n t L y m p h o cy te co u n t M o n o cy te co u n t E o sin o p h il c o u n t B a so p h il c o u n t A n iso c y to sis P o ly ch ro m asia P o ik ilo c y to sis T o x ic n e u tro p h ils Abbreviation (Units) R B C ( E 6 / U L o r X 1 0 6/ / / L ) H G B (G /D L ) H C T (% ) M C V (F L ) M C H (P G ) M C H C (% ) P L T ( E 3 / U L o r X 1 0 3/ / / L ) M P V (F L ) R E T IC (% ) R E T I C ( e 3 / U L o r X 1 0 3/ / / L ) H E IN Z (% ) E S R (M M /H R ) P T (S E C ) P T T (S E C ) T T (S E C ) A C T (S E C ) F B R (M G /D L ) F D P (U G /M l ) P A G G /C O L (% ) P A G G /A D P (% ) A N T IP L A S (% ) B L E T IM E (S E C ) M E T H G B (% ) P L A H G B (M G /D L ) M /E R A T IO E S T M /E R A T IO W B C ( E 3 / U L o r X 1 0 3/ / / L ) N - S E G ( E 3 / U L o r X 1 0 3/ / / L ) a n d % L Y M P H ( E 3 / U L o r X 1 0 3/ / / L ) a n d % M O N O ( E 3 / U L o r X 1 0 3/ / / L ) a n d % E O S I N ( E 3 / U L o r X 1 0 3/ / / L ) a n d % B A S O ( E 3 / U L o r X 1 0 3/ / / L ) a n d % A N IS O (-,1 ,2 ,3 ) P O L Y (-,1 ,2 ,3 ) P O IK (-,1 ,2 ,3 ) T O X N E U T (-,1 ,2 ,3 ,4 ) 91 Covance 6329-212 3M T-6316.1 Abbreviations and Units for Clinical Chemistry Test Glucose Urea nitrogen Creatinine Total protein Albumin Globulin Total bilirubin Cholesterol Triglyceride Aspartate aminotransferase Alanine aminotransferase Gamma glutamyltransferase Palmitoyl CoA oxidase Calcium Inorganic phosphorus Sodium Potassium Chloride Abbreviation (Units) GLU (MG/DL) UN (MG/DL) CREAT (MG/DL) T PRO (G/DL) ALB (G/DL) GLOB (G/DL) T BILI (MG/DL) CHOL (MG/DL) TRIG (MG/DL) AST/SGOT (IU/L) ALT/SGPT (IU/L) GGT (IU/L) PCOAO (IU/G) CA (MG/DL) I PHOS (MG/DL) NA (MMOL/L) K (MMOL/L) CL (MMOL/L) 92 Covance 6329-212 3M T-6316.1 Abbreviations and Units for Clinical Urinalysis Test Urine volume Specific gravity Urine chemistry Multistix strip Urine pH Urine protein Urine glucose Urine ketones Urine bilirubin Urine blood Urine urobilinogen Microscopic examination of urine sediment Red blood cells per high-power field White blood cells per high-power field Epithelial cells per high-power field Bacteria per high-power field Casts per low-power field Crystals per low-power field Urine appearance Comments Abbreviation (Units) U VOL (ML) SPGR UPH U PRO (MG/DL) U GLU UKET UBILI U BLOOD UROBILI RBC (PER HPF) WBC (PER HPF) EPITH (PER HPF) BACT (PER HPF) CASTS (PER LPF) CRYSTALS (PERLPF1 or PER LPF2) URINE APPI or URINE APP2 COMMENTS 93 Covance 6329-212 3M T-6316.1 Abbreviations and Units for Urine Chemistry Test Urine sodium Urine potassium Sodium excretion Potassium excretion Abbreviation (Units) U NA (MMOL/L) U K (MMOL/L) NA EXC (MMOL) K EXC (MMOL) 94 Code EX NOT TAKEN MISSING UNSUITABLE AUTOLYTIC EXCLUDE Covance 6329-212 3M T-6316.1 Codes for Anatomic Pathology Definition MACROSCOPIC CODES Indicates that organ weight is excluded from calculations Organ weight not taken; explanation given in necropsy notes Organ missing or lost Organ technically unsuitable for weighing Organ autolyzed and could not be weighed Weight was taken, but was excluded from all calculations MICROSCOPIC CODES Codes Prefacing Neoplastic Findings B- Primary, benign neoplasm M- Primary, malignant neoplasm N- Metastatic neoplasm I- Locally invasive neoplasm X- Other neoplasm Distribution of Findings Focal Diffuse Multifocal Grades for Severity or Amount 1 Minimal - the least amount of change that can be observed with the light microscope 2 Slight - less than average amount of change, but readily discernible as abnormal 3 Moderate - the average amount of change that is expected for a lesion 4 Moderately severe (marked) - a marked amount of change with possible loss of function of the affected cells or organs 5 Severe - a great amount of change with probable loss of function of the affected cell or organs and frequently involves large areas of the organ 95 Codes for Anatomic Pathology (Continued) Other Microscopic Codes TL Total P Finding present - Finding not present MN Mean Covance 6329-212 3M T-6316.1 TISSUE ABBREVIATIONS Abbreviation LN GL STOMACH, GL STOMACH, NONGL SALIV GL, MANDIB LN, ANT MES/PANC AUDITORY SEB GL LACRIMAL GLAND, EX HEMATO NEOPLASIA LACRIMAL GL, INT CAVITY, ABDOM SALIV GL,PAROTID LN, TRACHEOBRON Definition Lymph node Gland Glandular stomach Nonglandular stomach Mandibular salivary gland Anterior mesenteric/pancreatic lymph node Auditory sebaceous gland Exorbital lacrimal gland Hematopoietic neoplasia Internal lacrimal gland Abdominal cavity Parotid salivary gland Tracheobronchial lymph node 96 Figure 1 Adjusted Survival Data (%) - Males Covance 6329-212 3M T-6316.1 100 104-WEEK DIETARY CARCINOGENICITY STUDY W ITH NARROW RANGE (98.1%) N-ETHYL P ER FLUROOCTANESULFONAMID O ETH ANOL IN RATS 90 80 70 60 50 --- Group 1 - 0 ppm 40 -- Group 2 - 3 ppm A-- Group 3 - 3 0 ppm 30 Group 4 - 1 0 0 ppm -- Group 5 - 300 ppm 20 -- Group 6 - 1 0 0 ppm -I-- Group 7 - 300 ppm 10 0 0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104 Week (%) 97 Figure 2 Adjusted Survival Data (%) - Females 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 0 4 8 12 16 20 24 28 32 36 40 44 48 52 56 60 64 68 72 76 80 84 88 92 96 100 104 Week 98 Figure 3 Mean Body Weight Data (g) - Males Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 900 800 700 600 500 400 300 200 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 Recovery period for Group 6 started Week 53. Week 99 Figure 4 Mean Body Weight Data (g) - Females Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 550 500 450 400 p> 350 300 250 200 150 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 Recovery period for Group 6 started Week 53. W eek 100 Figure 5 Mean Food Consumption Data (g) - Males Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 215 205 195 185 p> 175 165 155 145 135 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 Recovery period for Group 6 started Week 53. W eek 101 Figure 6 Mean Food Consumption Data (g) - Females Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUROOCTANESULFONAMIDO ETHANOL IN RATS 170 160 150 140 130 120 110 100 90 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 Recovery period for Group 6 started Week 53. Week (g) 102 T ab le 1 Covance 6329-212 3M T-6316.1 R e su lts o f H o m o g e n e ity A n a ly se s (p p m ) M ix e d 9 /1 1 /9 7 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S a m p le L o c a tio n R e p lic a te 3 T -6 3 1 6 (ppm ) 30 100 300 Top 1 2 3 M ean 6 .2 8 3 8 .0 6 .3 5 3 2 .2 6 .2 4 3 3 .7 6 .2 9 (2 1 0 )a 3 4 .6 (1 1 5 ) 9 5 .3 9 0 .9 9 3 .5 9 3 .2 (9 3 .2 ) 322 277 296 2 9 8 (9 9 .3 ) M id d le 1 2 3 M ean 5 .1 7 5 .6 5 5 .8 9 5 .5 7 (1 8 6 ) 3 3 .9 3 4 .1 3 4 .9 3 4 .3 (1 1 4 ) 102 143 9 6 .8 114 (114) 286 271 266 2 7 4 (9 1 .3 ) B o tto m 1 2 3 M ean 6 .6 1 5 .2 4 5 .3 7 5 .7 4 (1 9 1 ) 3 2 .9 3 5 .9 3 2 .7 3 3 .8 (1 1 3 ) 109 108 9 9 .4 105 (105) 325 334 278 312 (104) a E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e o re tic a l 103 T ab le 1 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f H o m o g e n e ity A n a ly se s (p p m ) M ix e d 9 /2 9 /9 7 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S a m p le L o c a tio n R e p lic a te T -6 3 1 6 (ppm ) 3 Top 1 2 3 M ean 3 .9 8 4 .2 6 3 0 .0 1 2 .7 (4 2 3 )a M id d le 1 2 3 M ean 4 .0 0 4 .1 4 3 .8 7 4 .0 0 (1 3 3 ) B o tto m 1 2 3 M ean 4 .3 2 3 .9 8 4 .2 0 4 .1 7 (1 3 9 ) a E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th eo re tic al. 104 T ab le 1 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f H o m o g e n e ity A n a ly se s (p p m ) M ix e d 1 0 /1 5 /9 7 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S a m p le L o c a tio n R e p lic a te T -6 3 1 6 (ppm ) 3 Top 1 2 3 M ean 3 .1 8 2 .9 9 3 .2 3 3 .1 3 (1 0 4 )a M id d le 1 2 3 M ean 3 .4 4 3 .1 9 3 .3 2 3 .3 2 (1 1 1 ) B o tto m 1 2 3 M ean 3 .0 6 3 .9 8 3 .3 4 3 .4 6 (1 1 5 ) a E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th eo retical. 105 T ab le 1 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f H o m o g e n e ity A n a ly se s (p p m ) M ix e d 1 1 /7 /9 7 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S a m p le L o c a tio n R e p lic a te 3 T -6 3 1 6 (ppm ) 300 Top 1 2 3 M ean 2 .7 3 3 .1 9 3 .5 3 3 .1 5 (1 0 5 )a 296 305 302 301 (100) M id d le 1 2 3 M ean 3 .6 7 2 .5 1 2 .8 3 3 .0 0 (1 0 0 ) 304 300 297 300 (100) B o tto m 1 2 3 M ean 3 .1 2 2 .9 1 3 .1 2 3 .0 5 (1 0 2 ) 293 278 328 300 (100) a E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th e o re tic a l. 106 T ab le 1 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f H o m o g e n e ity A n a ly se s (p p m ) M ix e d 1 1 /1 3 /9 7 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S a m p le L o c a tio n R e p lic a te 3 T -6 3 1 6 (ppm ) 300 Top 1 2 3 M ean 2 .9 3 2 .8 6 2 .9 1 2 .9 0 (9 6 .7 )a 290 314 291 2 9 8 (9 9 .3 ) M id d le 1 2 3 M ean 2 .6 5 2 .9 0 2 .8 7 2 .8 1 (9 3 .7 ) 288 302 293 2 9 4 (9 8 .0 ) B o tto m 1 2 3 M ean 2 .6 6 2 .8 4 3 .4 0 2 .9 7 (9 9 .0 ) 284 269 308 2 8 7 (9 5 .7 ) a E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e o re tic a l. 107 T ab le 1 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f H o m o g e n e ity A n a ly se s (p p m ) M ix e d 1 /1 5 /9 8 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S a m p le L o c a tio n R e p lic a te 3 T -6 3 1 6 (ppm ) 30 100 300 Top 1 2 3 M ean 2 .8 9 2 .5 4 2 .6 3 2 .6 9 (8 9 .7 )a 3 1 .8 2 7 .9 2 9 .0 2 9 .6 (9 8 .7 ) 8 9 .4 9 3 .7 9 9 .2 9 4 .1 (9 4 .1 ) 275 278 294 2 8 2 (9 4 .0 ) M id d le 1 2 3 M ean 2 .6 7 2 .6 6 2 .8 3 2 .7 2 (9 0 .7 ) 2 8 .9 2 9 .8 2 7 .2 2 8 .6 (9 5 .3 ) 8 7 .4 8 2 .2 9 0 .8 8 6 .8 (8 6 .8 ) 280 300 289 2 9 0 (9 6 .7 ) B o tto m B o tto m b 1 2 3 M ean 1 2 3 M ean 3 .0 2 8 .4 9 3 .0 7 4 .8 6 (1 6 2 ) 2 7 .9 2 7 .0 2 8 .0 2 7 .6 (9 2 .0 ) 8 9 .1 101 105 9 8 .4 (9 8 .4 ) 291 283 278 2 8 4 (9 4 .7 ) 3 .6 8 2 .5 2 3 .1 9 3 .1 3 (1 0 4 ) - - - a E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e o re tic a l. b R eassay. 108 T ab le 1 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f H o m o g e n e ity A n a ly se s (p p m ) M ix e d 8 /2 6 /9 9 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S a m p le L o c a tio n R e p lic a te T -6 3 1 6 (ppm ) 100 Top 1 2 M ean 156 115 136 (13 6 )a M id d le 1 2 M ean 121 121 121 (121) B o tto m 1 2 M ean 123 145 134 (134) a E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th eo retical. 109 T ab le 1 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f H o m o g e n e ity A n a ly se s (p p m ) M ix e d 8 /2 6 /9 9 (R e an a ly sis) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S a m p le L o c a tio n R e p lic a te T -6 3 1 6 (ppm ) 100 Top 1 2 M ean 7 6 .5 6 6 .0 7 1 .3 (7 1 .3 )a M id d le 1 2 M ean 6 6 .0 7 9 .4 7 2 .7 (7 2 .7 ) B o tto m 1 2 M ean 6 3 .5 6 8 .9 6 6 .2 (6 6 .2 ) a E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th eo retical. 110 T ab le 2 Covance 6329-212 3M T-6316.1 R e su lts o f S tab ility A n a ly se s (p p m ) M ix e d 9 /1 1 /9 7 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S to ra g e C o n d itio n s R e p lic a te 3 T -6 3 1 6 (ppm ) 300 In itial 19 days, room tem p e ra tu re 1 2 M ean 1 2 M ean 7 .5 7 4 .7 0 6 .1 4 (2 0 5 )a 271 292 2 8 2 (9 4 .0 ) - 215 - 207 - 2 1 1 (7 0 .3 ) a E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th e o re tic a l. 111 T ab le 2 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f S tab ility A n a ly se s (p p m ) M ix e d 1 0 /1 5 /9 7 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S to ra g e C o n d itio n s R e p lic a te 3 T -6 3 1 6 (ppm ) 300 In itia la 1 2 M ean 3 .4 4 3 .1 9 3 .3 2 (1 1 1 )b 302 292 2 9 7 (9 9 .0 ) 19 days, room tem p e ra tu re 1 2 M ean 2 .8 3 3 .1 3 2 .9 8 (9 9 .3 ) 250 270 2 6 0 (8 6 .7 ) 34 days, room tem p e ra tu re 1 2 M ean 2 .9 0 3 .0 9 3 .0 0 (1 0 0 ) 266 266 2 6 6 (8 8 .7 ) 8 w eeks, frozen 1 2 M ean 3 .1 3 2 .8 0 2 .9 7 (9 9 .0 ) 275 291 2 8 3 (9 4 .3 ) a R e su lts w e re d eriv ed fro m th e sa m p le c o lle c te d fro m th e m id d le o f th e d o se p re p ara tio n s fo r h o m o g en e ity an aly sis. b E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e o re tic a l. 112 T ab le 2 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f S tab ility A n a ly se s (p p m ) M ix e d 9 /2 9 /9 7 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S to rag e C o n d itio n s R e p lic a te T -6 3 1 6 (ppm ) 3 In itial 1 2 M ean 4 .0 0 4 .4 7 4 .2 4 (1 4 1 )a a E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th eo retical. 113 T ab le 2 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f S tab ility A n a ly se s (p p m ) M ix e d 1 1 /1 3 /9 7 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S to rag e C o n d itio n s R e p lic a te 3 T -6 3 1 6 (ppm ) 300 5 days, room tem p e ra tu re 7 d ay s, a n im a l ro o m co n d itio n s 1 2 3 M ean 4 .1 6 3 .4 2 3 .0 0 3 .5 3 (1 1 8 )a 264 258 267 2 6 3 (8 7 .7 ) a E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th e o re tic a l. 114 T ab le 3 Covance 6329-212 3M T-6316.1 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 3 T -6 3 1 6 (ppm ) 30 100 300 1 -3 a 1 2 3 M ean b b - 2 .6 7 2 .6 6 2 .8 3 2 .7 2 (9 0 .7 )c 2 8 .9 2 9 .8 2 7 .2 2 8 .6 (9 5 .3 ) 8 7 .4 8 2 .2 9 0 .8 8 6 .8 (8 6 .8 ) 280 300 289 2 9 0 (9 6 .7 ) 4 -7 4-7d 1 2 M ean 1 2 M ean b b - - 5 .6 4 3 .1 0 4 .3 7 (1 4 6 ) 2 9 .0 2 7 .0 2 8 .0 (9 3 .3 ) 9 0 .4 9 7 .5 9 4 .0 (9 4 .0 ) 292 301 2 9 7 (9 9 .0 ) 2 .8 1 - - 3 .0 5 - - - 2 .9 3 (9 7 .7 ) - - - 8 -1 1 8 -1 1 d 1 2 -1 5 1 2 M ean 1 2 M ean b b - - 1 2 M ean b b - 4 .5 3 3 .4 5 3 .9 9 (1 3 3 ) 2 9 .9 2 8 .1 2 9 .0 (9 6 .7 ) 8 4 .3 8 6 .5 8 5 .4 (8 5 .4 ) 278 262 2 7 0 (9 0 .0 ) 2 .6 6 - - - 3 .2 0 - - - 2 .9 3 (9 7 .7 ) - - - 3 .3 4 2 .6 7 3 .0 1 (1 0 1 ) 2 6 .9 3 5 .6 3 1 .3 (1 0 4 ) 9 8 .2 9 9 .1 9 8 .7 (9 8 .7 ) - a R e su lts fo r th e 3-, 30 -, 100-, a n d 3 0 0 -p p m le v e ls w e re d e riv e d fro m th e sa m p le co lle cted fro m th e m id d le o f th e d o se p re p ara tio n s fo r h o m o g e n e ity an aly sis. b B e lo w th e lim it o f q u a n tita tio n (< 2 .0 0 p p m ). c E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e o re tic a l. d R eassay. 115 T ab le 3 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 3 T -6 3 1 6 (ppm ) 30 100 300 1 6 -1 9 1 6 -1 9 d 1 6 -1 9 f 2 0 -2 3 2 0 -23d 20-23f 1 e 3 .7 7 3 9 .3 8 7 .2 2 e 3 .0 4 3 1 .5 9 3 .0 - M ean - 3 .4 1 (1 1 4 )c 3 5 .4 (1 1 8 ) 9 0 .1 (9 0 .1 ) - 1 - 2 .7 8 2 1 .8 2 - 2 .2 0 4 4 .6 M e a n - 2 .4 9 (8 3 .0 ) 3 3 .2 (1 1 1 ) - - 1 - 2 .6 2 2 6 .9 2 - 2 .5 2 2 3 .3 M e a n - 2 .5 7 (8 5 .7 ) 2 5 .1 (8 3 .7 ) - - 1 e 2 .6 1 2 7 .7 101 - 2 e 2 .4 1 2 6 .4 9 2 .3 - M ean - 2 .5 1 (8 3 .7 ) 2 7 .1 (9 0 .3 ) 9 6 .7 (9 6 .7 ) - 1 - 2 .1 5 - 2 - 2 .4 7 - M ean - 2 .3 1 (7 7 .0 ) - ---- 1 - 2 .1 4 - 2 - 1 .9 0 - M ean - 2 .0 2 (6 7 .3 ) - ---- c E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e o re tic a l. d R eassay e B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ) f R e te n tio n . 116 T ab le 3 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 3 T -6 3 1 6 (ppm ) 30 100 300 24-27 2 4-27d 24-27f 2 8 -3 1 2 8-31d 28-31f 3 2 -3 5 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean e e - - - e e - - - e e - 2 .6 3 3 .0 4 2 .8 4 (9 4 .7 )c 2 4 .1 2 6 .7 2 5 .4 (8 4 .7 ) 8 3 .3 8 8 .4 8 5 .9 (8 5 .9 ) - 2 5 .3 - - 2 4 .0 - - 2 4 .7 (8 2 .3 )c - - 2 2 .4 - - 2 1 .4 - - 2 1 .9 (7 3 .0 ) - 2 .2 4 2 .5 2 2 .3 8 (7 9 .3 ) 3 .2 0 2 .6 0 2 .9 0 (9 6 .7 ) 3 .2 0 2 .7 3 2 .9 7 (9 9 .0 ) 2 6 .4 2 8 .4 2 7 .4 (9 1 .3 ) - - 8 7 .5 8 6 .0 8 6 .8 (8 6 .8 ) - - 2 .9 2 2 .4 6 2 .6 9 (8 9 .7 ) 2 8 .2 2 9 .7 2 9 .0 (9 6 .7 ) 8 3 .4 8 5 .9 8 4 .7 (8 4 .7 ) - - - - - - - c E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e o re tic a l. d R eassay. e B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ) f R e te n tio n . 117 T ab le 3 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 3 T -6 3 1 6 (ppm ) 30 100 300 36-39 36-39d 3 6 -3 9 f 4 0 -4 3 4 0-43d 44-47 4 4-47d 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean e e - - - e e - - e e - - 3 .3 6 2 .5 8 2 .9 7 (9 9 .0 )c 2 4 .9 2 2 .7 2 3 .8 (7 9 .3 ) 9 1 .4 8 7 .2 8 9 .3 (8 9 .3 ) 2 .5 3 2 .5 1 2 .5 2 (8 4 .0 ) 2 3 .0 2 2 .8 2 2 .9 (7 6 .3 ) - 2 .8 2 2 .6 8 2 .7 5 (9 1 .7 ) 2 7 .0 2 9 .6 2 8 .3 (9 4 .3 ) - 3 .0 1 5 .2 6 4 .1 4 (1 3 8 ) 2 8 .9 2 8 .3 2 8 .6 (9 5 .3 ) 9 5 .5 9 9 .5 9 7 .5 (9 7 .5 ) 2 .7 3 2 .7 1 2 .7 2 (9 0 .7 ) - - 3 .9 7 2 .7 7 3 .3 7 (1 1 2 ) 2 9 .3 2 7 .9 2 8 .6 (9 5 .3 ) 8 4 .4 9 5 .8 9 0 .1 (9 0 .1 ) 2 .9 3 2 .0 4 2 .4 9 (8 3 .0 ) - 6 4 .0 5 9 .6 6 1 .8 (6 1 .8 ) - - - - - - - c E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e o re tic a l. d R eassay. e B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ). f R e te n tio n . 118 T ab le 3 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 3 T -6 3 1 6 (ppm ) 30 100 300 44-47f 4 8 -5 1 5 2 -5 5 5 2 -5 5 d 52-55f 5 2 -5 5 g 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean - e e - e e - - - - 5 .3 0 2 .7 5 4 .0 3 (1 3 4 )c - 2 .6 7 2 .5 9 2 .6 3 (8 7 .7 ) 3 0 .2 2 9 .0 2 9 .6 (9 8 .7 ) 3 .2 2 3 .4 5 3 .3 4 (1 1 1 ) 2 5 .2 2 4 .0 2 4 .6 (8 2 .0 ) - 2 0 .4 - 2 1 .0 - 2 0 .7 (6 9 .0 ) - 1 9 .0 - 2 1 .5 - 2 0 .3 (6 7 .7 ) ---- 6 0 .4 5 8 .4 6 4 .4 (6 4 .4 ) 9 7 .8 9 4 .7 9 6 .3 (9 6 .3 ) 9 2 .5 9 7 .6 9 5 .1 (9 5 .1 ) - - 8 8 .4 8 4 .5 8 6 .5 (8 6 .5 ) - - - - - - c E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th e o re tic a l. d R eassay. e B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ). f R e te n tio n . g A d d itio n a l m ix . 119 T ab le 3 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 3 T -6 3 1 6 (ppm ) 30 100 300 5 6 -5 9 5 6 -5 9 d 56-59f 6 0 -6 3 6 0-63d 6 0 -6 3 f 64-67 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean e e - - - e e - - - e e - 3 .1 6 2 .4 3 2 .8 0 (9 3 .3 )c 4 9 .3 4 9 .0 4 9 .2 (1 6 4 ) 114 9 0 .6 102 (102) - 1 .7 1 1 .7 0 1 .71 (5 7 .0 ) 1 6 .0 1 5 .6 1 5 .8 (5 2 .7 ) 5 6 .0 5 8 .2 5 7 .1 (5 7 .1 ) - 1.7 5 1 .7 2 1 .7 4 (5 8 .0 ) 1 8 .6 1 7 .8 1 8 .2 (6 0 .7 ) 5 6 .8 5 4 .4 5 5 .6 (5 5 .6 ) - 2 .4 6 2 .5 6 2 .5 1 (8 3 .7 ) 2 8 .2 2 6 .4 2 7 .3 (9 1 .0 ) 8 7 .6 8 3 .2 8 5 .4 (8 5 .4 ) - 2 .4 8 2 .3 8 2 .4 3 (8 1 .0 ) - ---- 3 4 .8 3 .8 5 19 .3 (6 4 3 ) - ---- 2 .4 5 1 .9 7 2 .2 1 (7 3 .7 ) 1 7 .7 2 8 .0 2 2 .9 (7 6 .3 ) 7 3 .7 7 3 .4 7 3 .6 (7 3 .6 ) - c E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th e o re tic a l. d R eassay. e B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ). f R e te n tio n . 120 T ab le 3 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 3 T -6 3 1 6 (ppm ) 30 100 300 6 4-67d 6 4 -6 7 f 6 8 -7 1 6 8-71d 6 8 -7 1 f 7 2 -7 5 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 .5 9 2 0 .1 - 1 .5 8 1 8 .4 - 1 .5 9 (5 3 .0 )c 1 9 .3 (6 4 .3 ) - 1 .4 6 1 4 .9 - 3 .0 4 1 3 .6 - 2 .2 5 (7 5 .0 ) 1 4 .3 (4 7 .7 ) e 2 .5 1 2 5 .3 e 2 .1 8 2 4 .2 - 2 .3 5 (7 8 .3 )c 2 4 .8 (8 2 .7 ) - 2 .1 2 2 4 .3 - 2 .2 3 2 0 .6 - 2 .1 8 (7 2 .7 ) 2 2 .5 (7 5 .0 ) - 2 .3 1 2 0 .7 - 6 .0 0 2 4 .5 - 4 .1 6 (1 3 9 ) 2 2 .6 (7 5 .3 ) 1 .1 1 0 .6 2 3 0 .8 6 7 2 .6 3 1 .9 8 2 .3 1 (7 7 .0 ) 2 4 .0 2 8 .5 2 6 .3 (8 7 .7 ) 6 3 .6 6 6 .2 6 4 .9 (6 4 .9 ) - 5 5 .8 6 4 .4 6 0 .1 (6 0 .1 ) - 8 2 .5 8 2 .3 8 2 .4 (8 2 .4 ) - 8 0 .2 8 4 .1 8 2 .2 (8 2 .2 ) - 6 1 .6 6 2 .1 6 1 .9 (6 1 .9 ) - 8 6 .9 8 0 .8 8 3 .9 (8 3 .9 ) - c E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th e o re tic a l. d R eassay. e B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ) f R e te n tio n . 121 T ab le 3 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 3 T -6 3 1 6 (ppm ) 30 100 300 7 2-75d 1 2 M ean e e - 2 .0 2 2 2 .4 2 .2 5 2 1 .7 2 .1 4 (7 1 .3 )c 2 2 .1 (7 3 .7 ) 8 9 .0 8 5 .8 8 7 .4 (8 7 .4 ) - 7 2 -7 5 f 1 2 M ean e e - 2 .1 8 1 .9 6 2 .0 7 (6 9 .0 ) 2 1 .4 2 0 .2 2 0 .8 (6 9 .3 ) ---- 76-79 7 6-79d 1 2 M ean 1 2 M ean e e - e e - 2 .6 9 2 .4 2 2 .5 6 (8 5 .3 ) 2 5 .8 3 0 .6 2 8 .2 (9 4 .0 ) - 2 3 .1 - 2 3 .4 - 2 3 .3 (7 7 .7 ) 8 2 .0 9 3 .8 8 7 .9 (8 7 .9 ) - ---- 8 0 -8 3 1 2 M ean e e - 2 .3 5 2 .6 0 2 .4 8 (8 2 .7 ) 2 7 .2 2 4 .0 2 5 .6 (8 5 .3 ) 9 2 .8 8 6 .0 8 9 .4 (8 9 .4 ) - 8 4 -8 7 1 2 M ean e e - 3 .6 7 3 .8 6 3 .7 7 (1 2 6 ) 3 5 .1 4 4 .7 3 9 .9 (1 3 3 ) 121 121 121 (1 21) - c E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th e o re tic a l. d R eassay. e B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ) f R e te n tio n . 122 T ab le 3 (C o n tin u e d ) Covance 6329-212 3M T-6316.1 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 3 T -6 3 1 6 (ppm ) 30 100 300 8 8 -9 1 96-99 1 0 0 -1 0 3 1 0 0 -1 0 3 d 1 0 4 -1 0 5 1 0 4 -1 0 5 d 1 0 4 -1 0 5 d 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean 1 2 M ean e e - e e - e e - - e e - - - 2 .5 5 2 .4 9 2 .5 2 (8 4 .0 )c 3 2 .4 2 8 .2 3 0 .3 (1 0 1 ) 9 4 .2 9 2 .4 9 3 .3 (9 3 .3 ) - 2 .3 8 2 .4 4 2 .4 1 (8 0 .3 ) 3 4 .9 2 9 .7 3 2 .3 (1 0 8 ) 9 7 .4 8 8 .8 9 3 .1 (9 3 .1 ) - 2 .3 1 3 .4 1 2 .8 6 (9 5 .3 ) 3 6 .1 2 5 .5 3 0 .8 (1 0 3 ) 7 5 .3 9 0 .0 8 2 .7 (8 2 .7 ) - 1 .0 9 0 .9 1 4 1 .0 0 (3 3 .3 ) 6 8 .9 2 5 .6 4 7 .3 (1 5 8 ) 5 9 .7 6 1 .0 6 0 .4 (6 0 .4 ) - 4 .3 0 2 .2 6 3 .2 8 (1 0 9 ) 2 6 .1 2 8 .1 2 7 .1 (9 0 .3 ) 9 1 .1 9 5 .7 9 3 .4 (9 3 .4 ) - 1 .8 9 1 .8 8 1 .8 9 (6 3 .0 ) - ---- 1 .7 9 1 .8 2 1.8 1 (6 0 .3 ) - ---- c E a c h v a lu e in p a re n th e s e s is th e p e rc e n t o f th e o re tic a l. d R eassay. e B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ). f R e te n tio n . 123 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: START 70/70 100% 60/60 100% 60/60 100% 70/70 100% 70/70 100% 40/40 100% 40/40 100% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 1 70/70 100% 60/60 100% 60/60 100% 70/70 100% 70/70 100% 40/40 100% 40/40 100% 2 70/70 100% 60/60 100% 60/60 100% 70/70 100% 70/70 100% 40/40 100% 40/40 100% 3 70/70 100% 60/60 100% 60/60 100% 70/70 100% 70/70 100% 40/40 100% 40/40 100% 45 MALE 65/65 100% 55/55 100% 55/55 100% 65/65 100% 65/65 100% 40/40 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 65/65 100% 65/65 100% 40/40 100% 40/40 100% 6 65/65 100% 55/55 100% 55/55 100% 65/65 100% 65/65 100% 40/40 100% 39/40 98% 7 65/65 100% 55/55 100% 55/55 100% 65/65 100% 63/65 97% 39/40 98% 39/40 98% 8 60/60 100% 54/55 98% 55/55 100% 65/65 100% 0 0% 39/40 98% 0 0% 9 60/60 100% 54/55 98% 55/55 100% 65/65 100% 0 0% 39/40 98% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 1 10 11 60/60 100% 54/55 98% 55/55 100% 65/65 100% 0 0% 39/40 98% 0 0% 60/60 100% 54/55 98% 55/55 100% 65/65 100% 0 0% 39/40 98% 0 0% 124 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 12 60/60 100% 54/55 98% 55/55 100% 65/65 100% 0 0% 38/40 95% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 13 60/60 100% 54/55 98% 55/55 100% 64/65 98% 0 0% 38/40 95% 0 0% 14 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 15 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 16 17 MALE 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 18 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 19 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 20 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 21 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 2 22 23 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 125 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 24 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 25 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 26 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 27 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 28 29 MALE 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 30 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 31 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 32 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 33 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 3 34 35 55/55 100% 49/50 98% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 55/55 100% 48/50 96% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 126 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 36 55/55 100% 48/50 96% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 37 55/55 100% 48/50 96% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 38 55/55 100% 48/50 96% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 39 55/55 100% 48/50 96% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 40 41 MALE 55/55 100% 48/50 96% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 55/55 100% 48/50 96% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 42 55/55 100% 48/50 96% 50/50 100% 59/60 98% 0 0% 38/40 95% 0 0% 43 55/55 100% 48/50 96% 50/50 100% 59/60 98% 0 0% 37/40 93% 0 0% 44 54/55 98% 48/50 96% 50/50 100% 59/60 98% 0 0% 37/40 93% 0 0% 45 54/55 98% 48/50 96% 50/50 100% 58/60 97% 0 0% 37/40 93% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 4 46 47 54/55 98% 48/50 96% 50/50 100% 58/60 97% 0 0% 37/40 93% 0 0% 54/55 98% 48/50 96% 50/50 100% 58/60 97% 0 0% 37/40 93% 0 0% 127 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 48 54/55 98% 48/50 96% 50/50 100% 58/60 97% 0 0% 37/40 93% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 49 54/55 98% 48/50 96% 50/50 100% 58/60 97% 0 0% 37/40 93% 0 0% 50 54/55 98% 48/50 96% 50/50 100% 58/60 97% 0 0% 37/40 93% 0 0% 51 54/55 98% 48/50 96% 50/50 100% 58/60 97% 0 0% 36/40 90% 0 0% 52 53 MALE 54/55 98% 48/50 96% 50/50 100% 58/60 97% 0 0% 36/40 90% 0 0% 49/50 98% 48/50 96% 49/50 98% 48/50 96% 0 0% 36/40 90% 0 0% 54 49/50 98% 48/50 96% 49/50 98% 48/50 96% 0 0% 36/40 90% 0 0% 55 49/50 98% 48/50 96% 49/50 98% 48/50 96% 0 0% 36/40 90% 0 0% 56 49/50 98% 48/50 96% 48/50 96% 48/50 96% 0 0% 36/40 90% 0 0% 57 49/50 98% 47/50 94% 48/50 96% 48/50 96% 0 0% 36/40 90% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 5 58 59 49/50 98% 46/50 92% 48/50 96% 48/50 96% 0 0% 36/40 90% 0 0% 49/50 98% 45/50 90% 48/50 96% 47/50 94% 0 0% 36/40 90% 0 0% 128 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 60 49/50 98% 45/50 90% 48/50 96% 47/50 94% 0 0% 36/40 90% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 61 48/50 96% 44/50 88% 48/50 96% 47/50 94% 0 0% 35/40 88% 0 0% 62 47/50 94% 44/50 88% 47/50 94% 47/50 94% 0 0% 35/40 88% 0 0% 63 46/50 92% 44/50 88% 47/50 94% 47/50 94% 0 0% 35/40 88% 0 0% 64 65 MALE 46/50 92% 44/50 88% 47/50 94% 45/50 90% 0 0% 34/40 85% 0 0% 45/50 90% 44/50 88% 47/50 94% 45/50 90% 0 0% 34/40 85% 0 0% 66 44/50 88% 44/50 88% 47/50 94% 45/50 90% 0 0% 34/40 85% 0 0% 67 44/50 88% 44/50 88% 46/50 92% 45/50 90% 0 0% 34/40 85% 0 0% 68 44/50 88% 43/50 86% 46/50 92% 45/50 90% 0 0% 33/40 83% 0 0% 69 43/50 86% 43/50 86% 43/50 86% 42/50 84% 0 0% 33/40 83% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 6 70 71 42/50 84% 42/50 84% 43/50 86% 42/50 84% 0 0% 33/40 83% 0 0% 42/50 84% 41/50 82% 42/50 84% 41/50 82% 0 0% 33/40 83% 0 0% 129 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 72 40/50 80% 41/50 82% 41/50 82% 41/50 82% 0 0% 33/40 83% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 73 40/50 80% 41/50 82% 39/50 78% 40/50 80% 0 0% 32/40 80% 0 0% 74 40/50 80% 40/50 80% 39/50 78% 38/50 76% 0 0% 32/40 80% 0 0% 75 40/50 80% 40/50 80% 39/50 78% 38/50 76% 0 0% 32/40 80% 0 0% 76 77 MALE 40/50 80% 40/50 80% 38/50 76% 38/50 76% 0 0% 32/40 80% 0 0% 39/50 78% 39/50 78% 37/50 74% 38/50 76% 0 0% 32/40 80% 0 0% 78 39/50 78% 38/50 76% 36/50 72% 37/50 74% 0 0% 31/40 78% 0 0% 79 37/50 74% 38/50 76% 35/50 70% 36/50 72% 0 0% 31/40 78% 0 0% 80 36/50 72% 37/50 74% 34/50 68% 36/50 72% 0 0% 30/40 75% 0 0% 81 36/50 72% 34/50 68% 32/50 64% 36/50 72% 0 0% 30/40 75% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 7 82 83 36/50 72% 34/50 68% 32/50 64% 35/50 70% 0 0% 29/40 73% 0 0% 36/50 72% 33/50 66% 32/50 64% 35/50 70% 0 0% 26/40 65% 0 0% 130 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 84 35/50 70% 33/50 66% 32/50 64% 35/50 70% 0 0% 24/40 60% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 85 34/50 68% 33/50 66% 32/50 64% 34/50 68% 0 0% 22/40 55% 0 0% 86 33/50 66% 32/50 64% 32/50 64% 32/50 64% 0 0% 21/40 53% 0 0% 87 31/50 62% 32/50 64% 32/50 64% 32/50 64% 0 0% 21/40 53% 0 0% 88 89 MALE 30/50 60% 32/50 64% 32/50 64% 31/50 62% 0 0% 21/40 53% 0 0% 29/50 58% 32/50 64% 32/50 64% 31/50 62% 0 0% 20/40 50% 0 0% 90 29/50 58% 32/50 64% 32/50 64% 30/50 60% 0 0% 19/40 48% 0 0% 91 29/50 58% 32/50 64% 29/50 58% 30/50 60% 0 0% 19/40 48% 0 0% 92 28/50 56% 30/50 60% 29/50 58% 29/50 58% 0 0% 19/40 48% 0 0% 93 24/50 48% 28/50 56% 29/50 58% 28/50 56% 0 0% 16/40 40% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 8 94 95 23/50 46% 27/50 54% 28/50 56% 27/50 54% 0 0% 16/40 40% 0 0% 22/5 44% 27/5 54% 27/5 54% 27/5 54% 0 0% 16/4 40% 0 0% 131 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 96 21/50 42% 27/50 54% 27/50 54% 26/50 52% 0 0% 16/40 40% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 97 20/50 40% 27/50 54% 26/50 52% 26/50 52% 0 0% 15/40 38% 0 0% 98 19/50 38% 25/50 50% 25/50 50% 25/50 50% 0 0% 14/40 35% 0 0% 99 18/50 36% 24/50 48% 24/50 48% 25/50 50% 0 0% 14/40 35% 0 0% 100 101 MALE 15/50 30% 23/50 46% 24/50 48% 23/50 46% 0 0% 12/40 30% 0 0% 15/50 30% 22/50 44% 24/50 48% 21/50 42% 0 0% 12/40 30% 0 0% 102 14/50 28% 22/50 44% 24/50 48% 20/50 40% 0 0% 11/40 27% 0 0% 103 14/50 28% 22/50 44% 23/50 46% 18/50 36% 0 0% 10/40 25% 0 0% 104 14/5 28% 22/5 44% 22/5 44% 17/5 34% 0 0% 10/4 25% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 9 132 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: START 70/70 100% 60/60 100% 60/60 100% 70/70 100% 70/70 100% 40/40 100% 40/40 100% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 1 70/70 100% 60/60 100% 60/60 100% 70/70 100% 70/70 100% 40/40 100% 40/40 100% 2 70/70 100% 60/60 100% 60/60 100% 70/70 100% 70/70 100% 40/40 100% 40/40 100% 3 70/70 100% 60/60 100% 60/60 100% 70/70 100% 70/70 100% 40/40 100% 40/40 100% 45 FEMALE 65/65 100% 55/55 100% 55/55 100% 65/65 100% 65/65 100% 40/40 100% 40/40 100% 65/65 100% 55/55 100% 55/55 100% 65/65 100% 65/65 100% 40/40 100% 40/40 100% 6 65/65 100% 55/55 100% 55/55 100% 65/65 100% 65/65 100% 40/40 100% 40/40 100% 7 65/65 100% 55/55 100% 55/55 100% 65/65 100% 65/65 100% 40/40 100% 40/40 100% 8 60/60 100% 55/55 100% 55/55 100% 65/65 100% 0 0% 40/40 100% 0 0% 9 60/60 100% 55/55 100% 55/55 100% 65/65 100% 0 0% 40/40 100% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 10 10 11 60/60 100% 55/55 100% 55/55 100% 65/65 100% 0 0% 40/40 100% 0 0% 60/60 100% 55/55 100% 55/55 100% 65/65 100% 0 0% 40/40 100% 0 0% 133 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 12 60/60 100% 55/55 100% 55/55 100% 65/65 100% 0 0% 40/40 100% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 13 60/60 100% 55/55 100% 55/55 100% 65/65 100% 0 0% 40/40 100% 0 0% 14 55/55 100% 50/50 100% 50/50 100% 60/60 100% 0 0% 40/40 100% 0 0% 15 55/55 100% 50/50 100% 50/50 100% 59/59 100% 0 0% 40/40 100% 0 0% 16 17 FEMALE 55/55 100% 50/50 100% 50/50 100% 59/59 100% 0 0% 40/40 100% 0 0% 55/55 100% 50/50 100% 50/50 100% 59/59 100% 0 0% 40/40 100% 0 0% 18 55/55 100% 50/50 100% 50/50 100% 59/59 100% 0 0% 40/40 100% 0 0% 19 55/55 100% 50/50 100% 50/50 100% 59/59 100% 0 0% 40/40 100% 0 0% 20 55/55 100% 50/50 100% 50/50 100% 59/59 100% 0 0% 40/40 100% 0 0% 21 55/55 100% 50/50 100% 50/50 100% 59/59 100% 0 0% 40/40 100% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 11 22 23 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 134 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 24 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 25 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 26 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 27 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 28 29 FEMALE 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 30 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 31 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 32 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 33 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 12 34 35 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 135 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 36 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 37 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 38 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 39 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 40 41 FEMALE 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 42 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 43 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 44 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 45 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 13 46 47 55/55 100% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 54/55 98% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 136 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 48 54/55 98% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 49 54/55 98% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 50 54/55 98% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 51 54/55 98% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 52 53 FEMALE 54/55 98% 50/50 100% 50/50 100% 58/59 98% 0 0% 40/40 100% 0 0% 49/50 98% 50/50 100% 49/50 98% 48/49 98% 0 0% 40/40 100% 0 0% 54 49/50 98% 49/50 98% 49/50 98% 48/49 98% 0 0% 40/40 100% 0 0% 55 49/50 98% 49/50 98% 49/50 98% 48/49 98% 0 0% 40/40 100% 0 0% 56 49/50 98% 49/50 98% 49/50 98% 48/49 98% 0 0% 40/40 100% 0 0% 57 49/50 98% 49/50 98% 49/50 98% 48/49 98% 0 0% 40/40 100% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 14 58 59 48/50 96% 48/50 96% 49/50 98% 48/49 98% 0 0% 40/40 100% 0 0% 48/50 96% 48/50 96% 47/50 94% 47/49 96% 0 0% 40/40 100% 0 0% 137 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 60 48/50 96% 48/50 96% 47/50 94% 47/49 96% 0 0% 40/40 100% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 61 47/50 94% 47/50 94% 47/50 94% 47/49 96% 0 0% 40/40 100% 0 0% 62 46/50 92% 47/50 94% 47/50 94% 47/49 96% 0 0% 39/40 98% 0 0% 63 46/50 92% 47/50 94% 46/50 92% 46/49 94% 0 0% 38/40 95% 0 0% 64 65 FEMALE 45/50 90% 47/50 94% 46/50 92% 45/49 92% 0 0% 37/40 93% 0 0% 45/50 90% 47/50 94% 46/50 92% 45/49 92% 0 0% 37/40 93% 0 0% 66 45/50 90% 46/50 92% 46/50 92% 45/49 92% 0 0% 37/40 93% 0 0% 67 44/50 88% 44/50 88% 44/50 88% 45/49 92% 0 0% 37/40 93% 0 0% 68 44/50 88% 42/50 84% 44/50 88% 45/49 92% 0 0% 37/40 93% 0 0% 69 44/50 88% 42/50 84% 42/50 84% 44/49 90% 0 0% 36/40 90% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 15 70 71 44/50 88% 42/50 84% 41/50 82% 43/49 88% 0 0% 36/40 90% 0 0% 44/50 88% 42/50 84% 41/50 82% 42/49 86% 0 0% 35/40 88% 0 0% 138 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 72 44/50 88% 42/50 84% 38/50 76% 41/49 84% 0 0% 34/40 85% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 73 42/50 84% 40/50 80% 36/50 72% 41/49 84% 0 0% 33/40 83% 0 0% 74 41/50 82% 40/50 80% 34/50 68% 39/49 80% 0 0% 31/40 78% 0 0% 75 37/50 74% 36/50 72% 34/50 68% 38/49 78% 0 0% 31/40 78% 0 0% 76 77 FEMALE 36/50 72% 35/50 70% 34/50 68% 38/49 78% 0 0% 31/40 78% 0 0% 34/50 68% 34/50 68% 34/50 68% 38/49 78% 0 0% 30/40 75% 0 0% 78 33/50 66% 34/50 68% 34/50 68% 36/49 73% 0 0% 30/40 75% 0 0% 79 33/50 66% 33/50 66% 34/50 68% 35/49 71% 0 0% 30/40 75% 0 0% 80 32/50 64% 33/50 66% 34/50 68% 35/49 71% 0 0% 29/40 73% 0 0% 81 31/50 62% 33/50 66% 33/50 66% 35/49 71% 0 0% 28/40 70% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 16 82 83 31/50 62% 33/50 66% 33/50 66% 35/49 71% 0 0% 28/40 70% 0 0% 31/50 62% 32/50 64% 30/50 60% 34/49 69% 0 0% 26/40 65% 0 0% 139 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 84 30/50 60% 31/50 62% 30/50 60% 34/49 69% 0 0% 26/40 65% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 85 30/50 60% 30/50 60% 30/50 60% 33/49 67% 0 0% 25/40 63% 0 0% 86 29/50 58% 29/50 58% 28/50 56% 31/49 63% 0 0% 23/40 58% 0 0% 87 29/50 58% 27/50 54% 27/50 54% 30/49 61% 0 0% 22/40 55% 0 0% 88 89 FEMALE 27/50 54% 26/50 52% 27/50 54% 29/49 59% 0 0% 22/40 55% 0 0% 25/50 50% 26/50 52% 26/50 52% 29/49 59% 0 0% 22/40 55% 0 0% 90 23/50 46% 26/50 52% 26/50 52% 29/49 59% 0 0% 21/40 53% 0 0% 91 22/50 44% 25/50 50% 25/50 50% 29/49 59% 0 0% 21/40 53% 0 0% 92 22/50 44% 24/50 48% 25/50 50% 29/49 59% 0 0% 20/40 50% 0 0% 93 21/50 42% 24/50 48% 24/50 48% 28/49 57% 0 0% 17/40 43% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 17 94 95 19/50 38% 22/50 44% 22/50 44% 27/49 55% 0 0% 17/40 43% 0 0% 18/50 36% 22/50 44% 22/50 44% 27/49 55% 0 0% 17/40 43% 0 0% 140 GROUP AND DOSE LEVEL (PPM) 1 0 2 3 3 30 4 100 5 300 6 100 7 300 WEEK: 96 17/50 34% 21/50 42% 22/50 44% 27/49 55% 0 0% 15/40 38% 0 0% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 97 17/50 34% 17/50 34% 20/50 40% 25/49 51% 0 0% 14/40 35% 0 0% 98 15/50 30% 16/50 32% 19/50 38% 23/49 47% 0 0% 13/40 33% 0 0% 99 15/50 30% 15/50 30% 18/50 36% 23/49 47% 0 0% 13/40 33% 0 0% 100 101 FEMALE 15/50 30% 14/50 28% 17/50 34% 23/49 47% 0 0% 12/40 30% 0 0% 15/50 30% 14/50 28% 17/50 34% 21/49 43% 0 0% 12/40 30% 0 0% 102 15/50 30% 14/50 28% 17/50 34% 21/49 43% 0 0% 12/40 30% 0 0% 103 13/50 26% 14/50 28% 16/50 32% 19/49 39% 0 0% 12/40 30% 0 0% 104 13/50 26% 13/50 26% 15/50 30% 19/49 39% 0 0% 12/40 30% 0 0% Covance 6329-212 3M T-6316.1 PAGE: 18 141 DAYS 1-732 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** APPEARANCE BENT TAIL CONVULSIONS DEHYDRATED HEAD TILT LEFT RIGHT HUNCHED LIMITED USE LIMB-HIND-RIGHT LIMBS-FRONT LIMBS-HIND PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND MALOCCLUSION MISSING TAIL-PROXIMAL TEETH OBESE PARALYSIS LIMB-FRONT-LEFT LIMBS-HIND PROLAPSED VAGINA Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0001210 0000001 0002 010 101004 0 9 6 7 13 0 4 0 1000010 1000000 3001000 0000000 1000000 0001000 1000000 5 5 9 23 2 9 2 0000000 1114 01 0 0100000 0001010 0110000 0000000 142 Covance 6329-212 3M T-6316.1 PAGE: 1 Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DAYS 1-732 CATEGORY KEYWORD QUALIFIER SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 APPEARANCE PROTRUDING PENIS SWOLLEN DORSAL-CERVICAL INGUINAL REGION-LEFT INGUINAL REGION-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND MOUTH NOSE PAW-FRONT-LEFT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-CERVICAL THIN EXCESSIVE BLEEDING FROM PAWHR, CAUTERIZED WITH SILVER NITRATE BEHAVIOR ATAXIC CIRCLING LEFT 2121 1000 0000 0000 2100 0100 1000 0001 0100 0000 34 01 1010 0001 1211 0001 0000 1000 18 15 15 20 1000 00 00 00 00 00 00 00 03 00 00 02 01 00 00 00 01 00 1 11 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 02 5 01 0 0000000 143 Covance 6329-212 3M T-6316.1 PAGE: 2 DAYS 1-732 CATEGORY KEYWORD QUALIFIER BEHAVIOR CIRCLING RIGHT HYPOACTIVE RECUMBENT LATERAL STERNAL SENSITIVE TO TOUCH VOCALIZATION DISCHARGE GENITAL RED IN COLOR MUCOID ORAL RED IN COLOR CLEAR NASAL RED IN COLOR UNKNOWN RED IN COLOR EXCRETION DISCOLORED URINE RED IN COLOR ORANGE IN COLOR Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 15 8 11 15 1 8 1 5322 01 0 222102 0 2421030 0000100 1212 04 0 0000000 2102 001 0100010 333714 0 0000100 1001000 1000000 144 Covance 6329-212 3M T-6316.1 PAGE: 3 DAYS 1-732 CATEGORY KEYWORD QUALIFIER EXCRETION FEW FECES LIQUID FECES NO FECES NONFORMED FECES EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES CLOUDY DISCHARGE EYE-RIGHT DISCOLORED/DARK RED EYE-LEFT DILATED PUPIL EYES DISCOLORED/LIGHT RED EYE-LEFT EYE-RIGHT EYES OPAQUE EYE-LEFT EYE-RIGHT EYES Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 16 16 15 14 0000 10 4 9 9 9 10 8 6 0 14 01 05 02 1 0 0 0 1000000 0000000 1000000 0000000 0000000 0100000 1101000 0000010 0011000 0100000 1001000 12 00000 145 Covance 6329-212 3M T-6316.1 PAGE: 4 DAYS 1-732 CATEGORY KEYWORD QUALIFIER EYES PALE EYE-RIGHT EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SQUINTED PERI-ORBITAL PERI-ORBITAL-RIGHT RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE ALOPECIA BROWN HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT PERINEAL AREA SCROTUM VENTRAL-ABDOMINAL GENERALIZED Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -------------- MALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 1000000 2100010 2 032 03 0 42 02 030 9357050 5248050 0010000 2333000 1321030 6632130 0000000 0001000 0001000 3322 04 0 0101000 231604 0 011102 0 146 Covance 6329-212 3M T-6316.1 PAGE: 5 DAYS 1-732 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE BLUE SKIN PAW-HIND-LEFT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL BROWN SKIN PAWS-ALL VENTRAL-ABDOMINAL COLD TO TOUCH LIMBS-ALL LIMBS-HIND ENTIRE BODY BLACK SKIN VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-RIGHT PALE EARS ENTIRE BODY RED HAIR COAT DORSAL-CERVICAL DORSAL-CERVICAL-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -------------- MALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0100000 0000000 0000000 0000000 0000000 0010000 0001000 0000000 0000000 31 0312 0 0000000 0000000 0000000 2 12 312 0 0000000 0000000 44 7512 0 3121000 2000000 147 Covance 6329-212 3M T-6316.1 PAGE: 6 DAYS 1-732 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-RIGHT LIMBS-ALL LIMBS-FRONT PERINEAL AREA VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-CERVICAL VENTRAL-THORACIC ROUGH HAIR COAT SCALY SKIN TAIL TAIL-DISTAL SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT EAR-RIGHT EARS HEAD-ENTIRE Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -------------- MALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 1000000 0100000 1000000 0010000 4245001 0010000 0010010 1010000 34 14 03 0 0010000 0000000 1000000 2100000 2311000 1000000 0000000 0010000 0001000 0001000 0010000 0001000 0000011 148 Covance 6329-212 3M T-6316.1 PAGE: 7 DAYS 1-732 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE SORE/SCAB HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-LEFT LATERAL-CERVICAL-RIGHT LIMB-FRONT-LEFT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND LATERAL-THORACIC-RIGHT LUMBAR-LEFT MOUTH PAW-FRONT-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND SACRAL-LEFT SCROTUM SCROTUM-RIGHT SHOULDER-LEFT SHOULDER-RIGHT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -------------- MALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0100000 0001000 0112 000 1010000 0100000 1000000 1000000 0000000 032 0000 0100000 1000000 0000000 0000000 0001000 0010000 5 3 10 5 0 9 0 6684 04 0 0000000 535502 0 0000000 0011000 0010000 02 1 0 0 0 0 0000000 149 Covance 6329-212 3M T-6316.1 PAGE: 8 DAYS 1-732 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE SORE/SCAB TAIL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-RIGHT YELLOW HAIR COAT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 2221000 0100000 112302 0 0100000 0000000 12 0011 0 0010000 0010000 0100000 0000000 92 6 7 08 1 Covance 6329-212 3M T-6316.1 PAGE: 9 150 DAYS 1-732 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** APPEARANCE BENT TAIL CONVULSIONS DEHYDRATED HEAD TILT LEFT RIGHT HUNCHED LIMITED USE LIMB-HIND-RIGHT LIMBS-FRONT LIMBS-HIND PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND MALOCCLUSION MISSING TAIL-PROXIMAL TEETH OBESE PARALYSIS LIMB-FRONT-LEFT LIMBS-HIND PROLAPSED VAGINA Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000 0012 002 0 5231 6651 5 4 12 8 0000 0000 0000 2000 1000 0000 0000 10 19 12 19 0000 1100 0000 0000 0010 0000 01 00 03 02 01 04 00 00 00 00 00 00 00 0 11 01 00 00 00 00 01 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 151 Covance 6329-212 3M T-6316.1 PAGE: 10 DAYS 1-732 CATEGORY KEYWORD QUALIFIER APPEARANCE PROTRUDING PENIS SWOLLEN DORSAL-CERVICAL INGUINAL REGION-LEFT INGUINAL REGION-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND MOUTH NOSE PAW-FRONT-LEFT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-CERVICAL THIN GENITAL DISCHARGE, GREEN BEHAVIOR ATAXIC CIRCLING LEFT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000 0000 1000 1000 0100 0000 0000 1100 0000 0100 2 011 1100 0000 0000 0000 2100 0000 17 19 23 22 1000 00 00 00 00 00 01 00 02 01 00 00 00 00 00 00 02 00 0 16 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3371030 012 0000 152 Covance 6329-212 3M T-6316.1 PAGE: 11 DAYS 1-732 CATEGORY KEYWORD QUALIFIER BEHAVIOR CIRCLING RIGHT HYPOACTIVE RECUMBENT LATERAL STERNAL SENSITIVE TO TOUCH VOCALIZATION DISCHARGE GENITAL RED IN COLOR MUCOID ORAL RED IN COLOR CLEAR NASAL RED IN COLOR UNKNOWN RED IN COLOR EXCRETION DISCOLORED URINE RED IN COLOR ORANGE IN COLOR Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0100000 7 11 14 14 0 9 0 0111010 2210000 3101000 0100000 3112 04 0 0001000 0112 000 0011000 2 82 905 0 0000000 0010000 0000000 153 Covance 6329-212 3M T-6316.1 PAGE: 12 DAYS 1-732 CATEGORY KEYWORD QUALIFIER EXCRETION FEW FECES LIQUID FECES NO FECES NONFORMED FECES EYES CLEAR DISCHARGE EYE-LEFT EYE-RIGHT EYES CLOUDY DISCHARGE EYE-RIGHT DISCOLORED/DARK RED EYE-LEFT DILATED PUPIL EYES DISCOLORED/LIGHT RED EYE-LEFT EYE-RIGHT EYES OPAQUE EYE-LEFT EYE-RIGHT EYES Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 18 16 15 16 0000 6754 3000 0 10 00 09 02 0 0 0 0 102 0000 0000010 0001000 1000000 0010000 0000000 0000000 0001000 0000000 0000000 0000010 0000000 154 Covance 6329-212 3M T-6316.1 PAGE: 13 DAYS 1-732 CATEGORY KEYWORD QUALIFIER EYES PALE EYE-RIGHT EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SQUINTED PERI-ORBITAL PERI-ORBITAL-RIGHT RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE ALOPECIA BROWN HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT PERINEAL AREA SCROTUM VENTRAL-ABDOMINAL GENERALIZED Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 341302 0 3452 04 0 4222 02 0 11 16 12 7 0 8 0 22 32 01 0 0000000 04 4 6 03 0 04 1 1 01 0 5552 02 0 0012 04 0 0000000 0000000 1142 01 0 0000000 04 2 3 01 0 000002 0 155 Covance 6329-212 3M T-6316.1 PAGE: 14 DAYS 1-732 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE BLUE SKIN PAW-HIND-LEFT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL BROWN SKIN PAWS-ALL VENTRAL-ABDOMINAL COLD TO TOUCH LIMBS-ALL LIMBS-HIND ENTIRE BODY BLACK SKIN VENTRAL-ABDOMINAL-LEFT VENTRAL-THORACIC-RIGHT PALE EARS ENTIRE BODY RED HAIR COAT DORSAL-CERVICAL DORSAL-CERVICAL-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0010010 1100000 0010000 1000000 0000000 0000000 0010000 0100000 222102 0 0010000 0100010 02 00000 5432 04 0 0000010 0001000 5 10 6 10 0 4 0 0112 02 0 0000000 156 Covance 6329-212 3M T-6316.1 PAGE: 15 DAYS 1-732 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-RIGHT LIMBS-ALL LIMBS-FRONT PERINEAL AREA VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-CERVICAL VENTRAL-THORACIC ROUGH HAIR COAT SCALY SKIN TAIL TAIL-DISTAL SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT EAR-RIGHT EARS HEAD-ENTIRE Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 1002 000 22 0002 0 2216010 0000000 0000000 0001000 0022 060 0000010 0001000 1010000 2210000 1112 01 0 0100000 1000000 0000000 000002 0 0000000 0000000 0000000 0000010 157 Covance 6329-212 3M T-6316.1 PAGE: 16 DAYS 1-732 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE SORE/SCAB HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-LEFT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-LEFT LATERAL-CERVICAL-RIGHT LIMB-FRONT-LEFT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND LATERAL-THORACIC-RIGHT LUMBAR-LEFT MOUTH PAW-FRONT-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND SACRAL-LEFT SCROTUM SCROTUM-RIGHT SHOULDER-LEFT SHOULDER-RIGHT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0100000 0100000 0000000 1000000 0010000 0001000 0001000 1000000 1000000 1000000 2 00002 0 0001000 0001000 12 02 010 0000000 422 002 0 3112 01 0 0100000 4101030 0001000 0000000 0000000 0000000 0100000 158 Covance 6329-212 3M T-6316.1 PAGE: 17 DAYS 1-732 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE SORE/SCAB TAIL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-RIGHT YELLOW HAIR COAT *** END OF LIST *** Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000010 0000000 0010030 002 0000 012 003 0 1102 000 012 0000 0010000 0000000 0000010 8 7 15 10 0 8 0 Covance 6329-212 3M T-6316.1 PAGE: 18 159 CO CO DAYS 1-732 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT WEEK 26 WEEK 33 WEEK 43 WEEK 47 WEEK 56 WEEK 58 WEEK 59 WEEK 61 WEEK 63 WEEK 64 WEEK 65 WEEK 68 WEEK 71 WEEK 74 WEEK 75 WEEK 78 WEEK 81 WEEK 82 WEEK 84 WEEK 85 WEEK 86 WEEK 87 WEEK WEEK 89 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --- -MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 1000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0001000 0100000 160 Covance 6329-212 3M T-6316.1 PAGE: 1 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) AXILLARY REGION-LEFT WEEK 92 WEEK 97 WEEK 99 WEEK 100 WEEK 105 AXILLARY REGION-RIGHT WEEK 31 WEEK 35 WEEK 38 WEEK 45 WEEK 47 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 67 WEEK 68 WEEK 71 WEEK 74 WEEK 76 WEEK 82 WEEK 84 WEEK 85 WEEK 87 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0000000 2000000 0000000 0000010 161 Covance 6329-212 3M T-6316.1 PAGE: 2 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) AXILLARY REGION-RIGHT WEEK 88 WEEK 89 WEEK 91 WEEK 92 WEEK 93 WEEK 96 WEEK 97 WEEK 99 WEEK 102 WEEK 105 DORSAL WEEK 49 WEEK 56 WEEK 64 WEEK 68 WEEK 76 WEEK 82 WEEK 90 WEEK 98 WEEK 99 WEEK 103 WEEK 105 DORSAL-CERVICAL-LEFT WEEK 80 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0001000 0100000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0001000 0010000 0000000 0100000 0000000 0000000 0010000 0000000 0000000 0100000 0000000 162 Covance 6329-212 3M T-6316.1 PAGE: 3 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 HEAD-MAXILLARY-LEFT WEEK 80 WEEK 82 HEAD-MAXILLARY-RIGHT WEEK 17 WEEK 48 WEEK 51 WEEK 61 INGUINAL REGION-LEFT WEEK 64 WEEK 75 WEEK 76 WEEK 80 WEEK 81 WEEK 84 WEEK 86 WEEK 87 WEEK 88 WEEK 89 WEEK 92 WEEK 93 WEEK 94 WEEK 98 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -------------- MALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 1000000 0000000 0000000 0100000 0100000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 163 Covance 6329-212 3M T-6316.1 PAGE: 4 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) INGUINAL REGION-LEFT WEEK 105 INGUINAL REGION-RIGHT WEEK 72 WEEK 74 WEEK 75 WEEK 76 WEEK 80 WEEK 83 WEEK 84 WEEK 86 WEEK 87 WEEK 89 WEEK 92 WEEK 93 WEEK 94 WEEK 97 WEEK 98 LIMB-HIND-LEFT WEEK 25 WEEK 76 LIMB-HIND-RIGHT WEEK 71 WEEK 98 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 0000000 0000010 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0001000 0000000 0100000 164 Covance 6329-212 3M T-6316.1 PAGE: 5 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) MOUTH WEEK 20 WEEK 56 WEEK 67 WEEK 70 WEEK 71 WEEK 82 WEEK 85 WEEK 104 SHOULDER-LEFT WEEK 40 TAIL-PROXIMAL WEEK 86 VENTRAL-ABDOMINAL WEEK 33 WEEK 35 WEEK 40 WEEK 44 WEEK 49 WEEK 51 WEEK 57 WEEK 59 WEEK 62 WEEK 72 WEEK 74 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -------------- MALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 1000000 0000000 0001000 0000000 0000000 0010000 0000000 0000010 0010000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 165 Covance 6329-212 3M T-6316.1 PAGE: 6 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL -ABDOMINAL WEEK 77 WEEK 82 WEEK 83 WEEK 85 WEEK 88 WEEK 90 WEEK 92 WEEK 95 WEEK 102 VENTRAL -ABDOMINAL-LEFT WEEK 21 WEEK 22 WEEK 37 WEEK 45 WEEK 46 WEEK 49 WEEK 51 WEEK 53 WEEK 57 WEEK 61 WEEK 62 WEEK 64 WEEK 67 WEEK 71 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0100000 0000000 0010000 0000000 0000000 0000000 0000000 0000000 0001000 0100000 0000000 0001000 0000000 0000000 0000000 0100000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 166 Covance 6329-212 3M T-6316.1 PAGE: 7 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL -ABDOMINAL-LEFT WEEK 72 WEEK 74 WEEK 75 WEEK 76 WEEK 82 WEEK 84 WEEK 85 WEEK 86 WEEK 99 WEEK 100 WEEK 105 VENTRAL -ABDOMINAL-RIGHT WEEK 29 WEEK 32 WEEK 36 WEEK 38 WEEK 40 WEEK 49 WEEK 51 WEEK 53 WEEK 56 WEEK 62 WEEK 66 WEEK 70 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 0000000 0010000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0001000 0000000 0000000 0001000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 167 Covance 6329-212 3M T-6316.1 PAGE: 8 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL -ABDOMINAL-RIGHT WEEK 72 WEEK 81 WEEK 82 WEEK 84 WEEK 85 WEEK 88 WEEK 91 WEEK 94 WEEK 97 WEEK 98 WEEK 100 WEEK 105 VENTRAL -CERVICAL WEEK 62 WEEK 64 WEEK 68 WEEK 73 WEEK 75 WEEK 80 WEEK 95 WEEK 100 VENTRAL -CERVICAL-LEFT WEEK 43 WEEK 46 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0010000 1000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0000000 0000000 168 Covance 6329-212 3M T-6316.1 PAGE: 9 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 48 WEEK 61 WEEK 79 WEEK 87 WEEK 88 VENTRAL-CERVICAL-RIGHT WEEK 43 WEEK 61 WEEK 78 WEEK 105 VENTRAL-THORACIC WEEK 42 WEEK 54 WEEK 81 VENTRAL-THORACIC-LEFT WEEK 67 WEEK 79 WEEK 95 WEEK 101 VENTRAL-THORACIC-RIGHT WEEK 75 WEEK 84 HEAD WEEK 82 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -------------- MALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0000000 0000000 0001000 0000000 0000000 0000000 0000000 169 Covance 6329-212 3M T-6316.1 PAGE: 10 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) HEAD WEEK 86 WEEK 102 WART LIKE LESION(S) DORSAL WEEK 47 WEEK 51 WEEK 61 WEEK 69 WEEK 76 WEEK 79 WEEK 89 WEEK 95 WEEK 97 WEEK 105 DORSAL-CERVICAL-LEFT WEEK 105 EAR-LEFT WEEK 47 WEEK 65 WEEK 93 HEAD-MAXILLARY-LEFT WEEK 76 WEEK 89 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -------------- MALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000010 0100000 1000000 1000000 0010000 0100000 0010000 0000000 0000010 0100000 2000000 0010000 0100000 0000010 1000000 0100000 0000000 170 Covance 6329-212 3M T-6316.1 PAGE: 11 DAYS 1-732 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 67 MOUTH WEEK 21 WEEK 23 WEEK 31 WEEK 52 WEEK 83 WEEK 87 WEEK 91 WEEK 95 NOSE WEEK 79 PAW-FRONT-LEFT WEEK 95 PAW-FRONT-RIGHT WEEK 95 PAW-HIND-LEFT WEEK 89 PAW-HIND-RIGHT WEEK 34 SHOULDER-LEFT WEEK 37 TAIL WEEK 19 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 0000000 0000000 0000000 0000010 0100000 0100000 0001000 0000010 1000000 1000000 0010000 0100000 0010000 0100000 171 Covance 6329-212 3M T-6316.1 PAGE: 12 DAYS 1-732 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) TAIL WEEK 22 WEEK 82 WEEK 93 WEEK 104 TAIL-PROXIMAL WEEK 88 VENTRAL-ABDOMINAL WEEK 64 WEEK 69 WEEK 76 WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 79 VENTRAL-THORACIC WEEK 46 VENTRAL-THORACIC-RIGHT WEEK 69 WEEK 84 WEEK 87 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 012 0000 1000000 0100000 0100000 0000000 0000010 0100000 0000010 0100000 0100000 0000000 1000000 0100000 0100000 Covance 6329-212 3M T-6316.1 PAGE: 13 172 CO CO DAYS 1-732 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT WEEK 26 WEEK 33 WEEK 43 WEEK 47 WEEK 56 WEEK 58 WEEK 59 WEEK 61 WEEK 63 WEEK 64 WEEK 65 WEEK 68 WEEK 71 WEEK 74 WEEK 75 WEEK 78 WEEK 81 WEEK 82 WEEK 84 WEEK 85 WEEK 86 WEEK 87 WEEK WEEK 89 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 1000000 0010000 0010000 0100000 1000000 1010000 1100000 0000000 1001000 0010000 1000000 02 00000 1010010 2 011 000 1010000 0000010 0010000 1010000 0010000 1101000 0001000 0010010 0000000 0000000 173 Covance 6329-212 3M T-6316.1 PAGE: 14 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) AXILLARY REGION-LEFT WEEK 92 WEEK 97 WEEK 99 WEEK 100 WEEK 105 AXILLARY REGION-RIGHT WEEK 31 WEEK 35 WEEK 38 WEEK 45 WEEK 47 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 67 WEEK 68 WEEK 71 WEEK 74 WEEK 76 WEEK 82 WEEK 84 WEEK 85 WEEK 87 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0101000 1010000 1000000 0001000 0010000 0010000 0100000 2200000 0001000 1000000 0001000 0 02 1 0 0 0 1000000 1100000 1000000 1000010 1100000 02 00000 1100000 0110000 0101000 1 02 1 0 0 0 1110000 174 Covance 6329-212 3M T-6316.1 PAGE: 15 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) AXILLARY REGION-RIGHT WEEK 88 WEEK 89 WEEK 91 WEEK 92 WEEK 93 WEEK 96 WEEK 97 WEEK 99 WEEK 102 WEEK 105 DORSAL WEEK 49 WEEK 56 WEEK 64 WEEK 68 WEEK 76 WEEK 82 WEEK 90 WEEK 98 WEEK 99 WEEK 103 WEEK 105 DORSAL-CERVICAL-LEFT WEEK 80 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 1000000 0011000 0000000 0000010 012 0000 0010000 0010000 0010000 1000000 0010000 0000000 0000000 0000000 0001000 0000000 1000000 0011000 0000000 0001000 0001000 0000000 0001000 175 Covance 6329-212 3M T-6316.1 PAGE: 16 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 HEAD-MAXILLARY-LEFT WEEK 80 WEEK 82 HEAD-MAXILLARY-RIGHT WEEK 17 WEEK 48 WEEK 51 WEEK 61 INGUINAL REGION-LEFT WEEK 64 WEEK 75 WEEK 76 WEEK 80 WEEK 81 WEEK 84 WEEK 86 WEEK 87 WEEK 88 WEEK 89 WEEK 92 WEEK 93 WEEK 94 WEEK 98 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0010000 0010000 0000000 0001000 0001000 0000000 0000000 12 00000 0110000 1010000 0011000 0010000 02 1 0 0 0 0 02 00000 1100010 0010000 0100000 0000010 0010000 0100000 0000010 176 Covance 6329-212 3M T-6316.1 PAGE: 17 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) INGUINAL REGION-LEFT WEEK 105 INGUINAL REGION-RIGHT WEEK 72 WEEK 74 WEEK 75 WEEK 76 WEEK 80 WEEK 83 WEEK 84 WEEK 86 WEEK 87 WEEK 89 WEEK 92 WEEK 93 WEEK 94 WEEK 97 WEEK 98 LIMB-HIND-LEFT WEEK 25 WEEK 76 LIMB-HIND-RIGHT WEEK 71 WEEK 98 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0011000 1100000 0100000 0000000 1100000 0100000 0100000 02 00010 0010000 1100000 0010000 0010000 0100000 1000000 0100000 1100000 1000000 0000000 1000000 0000000 177 Covance 6329-212 3M T-6316.1 PAGE: 18 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) MOUTH WEEK 20 WEEK 56 WEEK 67 WEEK 70 WEEK 71 WEEK 82 WEEK 85 WEEK 104 SHOULDER-LEFT WEEK 40 TAIL-PROXIMAL WEEK 86 VENTRAL-ABDOMINAL WEEK 33 WEEK 35 WEEK 40 WEEK 44 WEEK 49 WEEK 51 WEEK 57 WEEK 59 WEEK 62 WEEK 72 WEEK 74 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000010 0000000 0001000 1000000 0000000 0100000 0000000 0000000 0010000 0001000 02 00010 0100000 0000010 0100000 1100000 0010000 0000010 0000010 0010000 0000010 178 Covance 6329-212 3M T-6316.1 PAGE: 19 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL -ABDOMINAL WEEK 77 WEEK 82 WEEK 83 WEEK 85 WEEK 88 WEEK 90 WEEK 92 WEEK 95 WEEK 102 VENTRAL -ABDOMINAL-LEFT WEEK 21 WEEK 22 WEEK 37 WEEK 45 WEEK 46 WEEK 49 WEEK 51 WEEK 53 WEEK 57 WEEK 61 WEEK 62 WEEK 64 WEEK 67 WEEK 71 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0100000 0000000 0001000 0100000 1010000 0100000 1000000 0000010 0000000 0010000 0000000 1000000 0100010 0100000 0000010 0010000 0010000 0000010 0010000 0100000 0001010 0111000 179 Covance 6329-212 3M T-6316.1 PAGE: 20 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL -ABDOMINAL-LEFT WEEK 72 WEEK 74 WEEK 75 WEEK 76 WEEK 82 WEEK 84 WEEK 85 WEEK 86 WEEK 99 WEEK 100 WEEK 105 VENTRAL -ABDOMINAL-RIGHT WEEK 29 WEEK 32 WEEK 36 WEEK 38 WEEK 40 WEEK 49 WEEK 51 WEEK 53 WEEK 56 WEEK 62 WEEK 66 WEEK 70 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0010000 0001000 0100000 0000000 02 00000 0002 000 0001000 1000000 0010000 0010000 0010000 0000000 1000000 1000000 0010000 0010000 02 00000 1011010 0010000 0100000 002 0000 0000010 0100000 180 Covance 6329-212 3M T-6316.1 PAGE: 21 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 72 WEEK 81 WEEK 82 WEEK 84 WEEK 85 WEEK 88 WEEK 91 WEEK 94 WEEK 97 WEEK 98 WEEK 100 WEEK 105 VENTRAL-CERVICAL WEEK 62 WEEK 64 WEEK 68 WEEK 73 WEEK 75 WEEK 80 WEEK 95 WEEK 100 VENTRAL-CERVICAL-LEFT WEEK 43 WEEK 46 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000010 0000000 0010000 0000010 0001000 0001000 1000000 0001000 0101000 1000000 0001000 0100000 1010000 0100000 0010000 0001000 0001000 0000000 0100000 1000000 0000010 0010000 181 Covance 6329-212 3M T-6316.1 PAGE: 22 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 48 WEEK 61 WEEK 79 WEEK 87 WEEK 88 VENTRAL-CERVICAL-RIGHT WEEK 43 WEEK 61 WEEK 78 WEEK 105 VENTRAL-THORACIC WEEK 42 WEEK 54 WEEK 81 VENTRAL-THORACIC-LEFT WEEK 67 WEEK 79 WEEK 95 WEEK 101 VENTRAL-THORACIC-RIGHT WEEK 75 WEEK 84 HEAD WEEK 82 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000010 0001000 0000010 0000010 1000000 0001000 0001000 0100000 0010000 0000000 0001000 0010000 0002 000 0010000 0000000 1000000 0000010 0100000 0100000 182 Covance 6329-212 3M T-6316.1 PAGE: 23 DAYS 1-732 CATEGORY KEYWORD QUALIFIER MASS(ES) HEAD WEEK 86 WEEK 102 WART LIKE LESION(S) DORSAL WEEK 47 WEEK 51 WEEK 61 WEEK 69 WEEK 76 WEEK 79 WEEK 89 WEEK 95 WEEK 97 WEEK 105 DORSAL-CERVICAL-LEFT WEEK 105 EAR-LEFT WEEK 47 WEEK 65 WEEK 93 HEAD-MAXILLARY-LEFT WEEK 76 WEEK 89 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 1000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0001000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 183 Covance 6329-212 3M T-6316.1 PAGE: 24 DAYS 1-732 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 67 MOUTH WEEK 21 WEEK 23 WEEK 31 WEEK 52 WEEK 83 WEEK 87 WEEK 91 WEEK 95 NOSE WEEK 79 PAW-FRONT-LEFT WEEK 95 PAW-FRONT-RIGHT WEEK 95 PAW-HIND-LEFT WEEK 89 PAW-HIND-RIGHT WEEK 34 SHOULDER-LEFT WEEK 37 TAIL WEEK 19 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0100000 1000000 0001000 0001000 0100000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 184 Covance 6329-212 3M T-6316.1 PAGE: 25 DAYS 1-732 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) TAIL WEEK 22 WEEK 82 WEEK 93 WEEK 104 TAIL-PROXIMAL WEEK 88 VENTRAL-ABDOMINAL WEEK 64 WEEK 69 WEEK 76 WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 79 VENTRAL-THORACIC WEEK 46 VENTRAL-THORACIC-RIGHT WEEK 69 WEEK 84 WEEK 87 *** END OF LIST *** Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED ------------- FEMALE 1234567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 0000000 0000000 0001000 0000000 0000000 0000000 0000000 0000000 0010000 0000000 0000000 0000000 Covance 6329-212 3M T-6316.1 PAGE: 26 185 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------------------------------------- MALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 1N 70 MEAN 261 S.D. 20.4 2N 70 MEAN 308 S.D. 23.1 3N 70 MEAN 350 S.D. 25.7 4N 70 MEAN 387 S.D. 29.0 5N 65 MEAN 417 S.D. 31.5 6N 65 MEAN 444 S.D. 33.6 7N 65 MEAN 467 S.D. 37.0 60 260 19.9 60 307 22.5 60 351 26.6 60 389 29.9 55 416 31.5 55 444 33.9 55 467 37.1 60 260 20.5 60 302 24.5 60 344 29.9 60 379 34.8 55 404 38.2 55 431 40.5 55 450 42.6 70 258 19.2 70 298 * 21.7 70 337 * 25.3 70 367 * 28.3 65 393 * 30.5 65 418 * 33.6 65 436 * 35.5 69 257 19.0 70 288 * 23.0 70 318 * 25.9 70 338 * 28.8 64 355 * 31.8 65 370 * 35.3 65 380 * 37.6 40 254 17.8 40 296 * 20.0 40 334 * 24.2 40 364 * 27.8 40 391 * 32.5 40 416 * 36.2 40 427 * 42.8 40 256 19.2 40 286 * 21.2 40 313 * 24.0 40 334 * 27.3 40 351 * 30.3 40 366 * 33.1 39 374 * 33.9 Covance 6329-212 3M T-6316.1 PAGE: 1 186 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------------------------------------- MALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 8N 65 MEAN 483 S.D. 39.5 9N 60 MEAN 504 S.D. 43.4 10 N 60 MEAN 521 S.D. 45.1 11 N 60 MEAN 532 S.D. 45.4 12 N 60 MEAN 543 S.D. 46.8 13 N 60 MEAN 553 S.D. 47.7 14 N 60 MEAN 563 S.D. 50.1 55 480 40.0 54 507 40.2 54 524 41.1 54 537 43.3 54 550 44.6 54 559 48.3 54 567 48.0 55 464 * 43.4 55 483 * 45.9 55 497 * 49.3 55 510 * 49.8 55 522 51.5 55 531 52.7 55 538 * 54.5 65 449 * 39.4 65 465 * 40.9 65 477 * 41.8 65 486 * 43.7 65 496 * 46.9 65 504 * 51.2 64 513 * 45.3 63 386 * 42.4 0 0 0 0 0 0 39 448 * 44.8 39 464 * 51.0 39 473 * 57.6 39 484 * 62.4 39 496 * 64.9 38 515 * 44.9 38 522 * 45.5 39 384 * 38.5 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 2 187 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------------------------------------- MALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 15 N 55 49 50 59 0 38 0 MEAN 569 574 540 * 519 * 528 * S.D. 51.8 50.9 51.9 45.8 47.5 16 N 55 49 50 59 0 38 0 MEAN 578 585 547 * 528 * 536 * S.D. 52.9 52.3 53.1 45.6 48.4 17 N 55 49 50 59 0 38 0 MEAN 590 595 557 * 536 * 546 * S.D. 55.0 54.0 53.4 46.4 50.2 21 N 55 49 50 59 0 38 0 MEAN 619 625 582 * 555 * 567 * S.D. 58.8 58.3 57.0 51.4 55.5 25 N 55 49 50 59 0 38 0 MEAN 644 651 603 * 572 * 582 * S.D. 62.8 64.2 62.1 51.3 60.7 29 N 55 49 50 59 0 38 0 MEAN 663 668 618 * 590 * 602 * S.D. 67.1 69.6 66.5 54.7 64.8 33 N 55 49 50 59 0 38 0 MEAN 678 682 634 * 599 * 612 * S.D. 71.5 72.0 70.2 57.5 64.5 Covance 6329-212 3M T-6316.1 PAGE: 3 188 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 37 N 55 MEAN 694 S.D. 77.1 41 N 55 MEAN 700 S.D. 71.4 45 N 54 MEAN 723 S.D. 76.2 49 N 54 MEAN 741 S.D. 78.3 53 N 54 MEAN 762 S.D. 81.9 57 N 49 MEAN 769 S.D. 87.5 61 N 49 MEAN 781 S.D. 92.9 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS --- MALE---234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 48 50 59 0 38 0 701 646 * 610 * 623 * 75.1 74.0 57.6 67.4 48 50 59 0 38 0 714 653 * 619 * 632 * 75.2 77.1 60.1 68.2 48 50 58 0 37 0 740 678 * 639 * 650 * 82.8 82.6 64.9 76.4 48 50 58 0 37 0 758 698 * 654 * 666 * 91.5 87.5 68.7 80.4 48 50 58 0 36 0 777 716 * 672 * 688 * 95.0 92.5 68.4 86.3 48 48 48 0 36 0 777 726 683 * 707 * 118.4 87.5 68.7 93.6 45 48 47 0 36 0 802 739 689 * 721 * 121.7 96.7 76.1 114.9 Covance 6329-212 3M T-6316.1 PAGE: 4 189 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------------------------------------- MALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 65 N 46 MEAN 787 S.D. 98.7 69 N 44 MEAN 789 S.D. 104.8 73 N 40 MEAN 811 S.D. 98.5 77 N 40 MEAN 799 S.D. 123.4 81 N 36 MEAN 799 S.D. 120.9 85 N 35 MEAN 791 S.D. 134.2 89 N 30 MEAN 791 S.D. 134.6 44 815 117.5 43 813 129.4 41 829 128.1 40 827 128.2 37 820 137.6 33 837 130.2 32 840 134.2 47 742 104.0 46 737 112.6 41 757 99.1 38 746 114.3 34 753 121.4 32 772 95.1 32 763 102.7 45 695 * 72.9 44 685 * 93.7 41 707 * 95.7 38 719 * 93.0 36 725 * 88.7 35 721 * 90.5 31 724 97.4 0 34 747 95.5 0 33 767 103.5 0 33 784 115.4 0 32 789 127.8 0 30 780 144.0 0 24 754 151.5 0 21 760 154.9 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 5 190 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 93 N MEAN S.D. 97 N MEAN S.D. 101 N MEAN S.D. 105 N MEAN S.D. 27 763 165.6 21 791 168.3 15 805 161.0 14 795 185.0 Table 7 Summary of Body Weight Data (g) 104 -WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ---MALE----234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 30 828 145.8 27 852 136.8 23 821 145.5 22 781 173.4 29 778 109.4 27 757 132.1 24 764 135.9 22 766 138.2 29 735 93.7 26 724 93.4 23 698 99.5 17 688 123.6 0 19 758 170.7 0 16 788 160.6 0 12 802 157.7 0 10 806 179.0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 6 191 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 1N 70 MEAN 165 S.D. 13.2 2N 70 MEAN 188 S.D. 16.9 3N 70 MEAN 211 S.D. 20.8 4N 70 MEAN 229 S.D. 22.3 5N 65 MEAN 244 S.D. 25.4 6N 65 MEAN 258 S.D. 28.3 7N 65 MEAN 267 S.D. 30.0 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS --FEMALE--234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 60 163 13.3 60 185 14.7 60 205 18.5 60 224 20.2 55 236 21.2 55 246 22.7 55 256 23.8 60 165 12.9 60 185 14.4 60 205 16.5 60 222 18.3 55 233 * 19.9 55 245 21.2 55 253 22.7 70 164 12.1 70 181 * 13.5 70 200 * 15.5 70 215 * 17.1 65 225 * 18.6 65 235 * 19.4 65 239 * 19.6 70 164 12.6 70 172 * 13.3 70 182 * 15.1 70 191 * 15.2 65 196 * 16.4 65 202 * 16.6 65 205 * 16.5 39 164 12.5 40 181 14.1 40 202 15.8 40 214 * 15.9 40 223 * 16.7 40 234 * 17.3 40 240 * 17.4 40 163 12.0 40 175 * 11.7 40 186 * 12.2 40 196 * 12.7 40 200 * 13.3 40 206 * 14.4 40 210 * 13.2 Covance 6329-212 3M T-6316.1 PAGE: 7 192 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ------------------------------------- FEMALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 8N 65 MEAN 274 S.D. 30.4 9N 60 MEAN 283 S.D. 32.1 10 N 60 MEAN 290 S.D. 33.6 11 N 60 MEAN 295 S.D. 35.1 12 N 60 MEAN 302 S.D. 36.6 13 N 60 MEAN 306 S.D. 36.3 14 N 60 MEAN 308 S.D. 36.3 55 261 24.9 55 271 25.6 55 277 27.3 55 281 28.7 55 284 28.6 55 289 27.5 55 292 * 27.9 55 258 * 23.6 55 268 * 24.1 55 273 * 25.1 55 275 * 26.3 55 280 * 26.7 55 284 * 26.6 55 286 * 27.7 65 246 * 20.7 65 250 * 20.7 65 254 * 20.3 65 256 * 21.8 65 258 * 21.4 65 260 * 21.7 65 262 * 22.3 65 212 * 17.1 0 0 0 0 0 0 40 243 * 19.4 40 250 * 18.9 40 253 * 21.5 40 255 * 19.9 40 259 * 20.8 40 261 * 20.2 40 264 * 20.8 40 213 * 14.9 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 8 193 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ------------------------------------- FEMALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 15 N 55 50 50 60 0 40 0 MEAN 310 295 * 288 * 263 * 266 * S.D. 36.0 29.0 27.9 23.3 21.9 16 N 55 50 50 59 0 40 0 MEAN 316 300 * 293 * 266 * 269 * S.D. 36.8 31.0 28.2 23.0 21.7 17 N 55 50 50 59 0 40 0 MEAN 320 306 297 * 270 * 272 * S.D. 37.9 30.2 29.3 23.2 22.2 21 N 55 50 50 59 0 40 0 MEAN 334 318 311 * 277 * 279 * S.D. 41.9 33.7 32.8 24.8 23.3 25 N 55 50 50 58 0 40 0 MEAN 348 334 324 * 286 * 291 * S.D. 47.6 38.7 37.2 26.4 24.5 29 N 55 50 50 58 0 40 0 MEAN 361 346 336 * 294 * 299 * S.D. 51.9 42.7 40.2 28.2 26.6 33 N 55 50 50 58 0 40 0 MEAN 375 359 349 301 * 307 * S.D. 55.1 48.3 44.1 31.8 30.0 Covance 6329-212 3M T-6316.1 PAGE: 9 194 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 37 N 55 MEAN 384 S.D. 58.2 41 N 55 MEAN 400 S.D. 64.7 45 N 55 MEAN 423 S.D. 72.3 49 N 54 MEAN 439 S.D. 76.0 53 N 54 MEAN 454 S.D. 82.1 57 N 49 MEAN 465 S.D. 93.3 61 N 48 MEAN 473 S.D. 98.7 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS -- FEMALE--234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 50 50 58 0 40 0 366 355 * 304 * 313 * 48.9 46.4 34.0 32.2 50 50 58 0 40 0 380 370 313 * 323 * 52.3 48.2 36.5 34.8 50 50 58 0 40 0 397 388 320 * 332 * 59.3 54.0 40.5 38.0 50 50 58 0 40 0 412 396 * 329 * 339 * 63.2 55.3 43.1 40.6 50 50 58 0 40 0 425 404 * 338 * 350 * 67.9 62.5 48.7 44.3 49 49 48 0 40 0 435 411 * 348 * 368 * 69.0 66.3 52.0 49.3 48 47 47 0 40 0 450 426 * 359 * 402 * 73.5 70.5 58.1 55.3 Covance 6329-212 3M T-6316.1 PAGE: 10 195 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 65 N 45 MEAN 483 S.D. 101.3 69 N 44 MEAN 492 S.D. 100.2 73 N 44 MEAN 487 S.D. 113.9 77 N 36 MEAN 500 S.D. 116.1 81 N 32 MEAN 513 S.D. 115.0 85 N 30 MEAN 506 S.D. 110.8 89 N 27 MEAN 496 S.D. 115.1 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS -- FEMALE--234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 47 46 45 0 37 0 455 430 * 368 * 424 * 75.9 75.7 61.2 57.3 42 44 45 0 37 0 470 434 * 371 * 433 * 78.5 85.2 65.9 73.9 42 480 79.1 38 451 92.7 41 381 * 67.7 0 34 459 70.7 0 35 34 38 0 31 486 469 377 * 474 87.4 85.8 71.0 65.8 0 33 34 35 0 29 502 469 381 * 480 85.4 93.6 77.1 80.8 0 31 30 34 0 26 501 483 382 * 473 97.5 94.6 75.7 91.2 0 26 504 96.9 27 484 94.3 29 387 * 72.9 0 22 480 115.1 0 Covance 6329-212 3M T-6316.1 PAGE: 11 196 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 93 N MEAN S.D. 97 N MEAN S.D. 101 N MEAN S.D. 105 N MEAN S.D. 22 515 117.2 17 513 105.9 15 515 110.1 13 492 113.3 Table 7 Summary of Body Weight Data (g) 104 -WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS --FEMALE---234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 24 25 29 0 20 495 496 390 * 502 94.6 103.3 76.0 106.0 21 22 27 0 15 488 515 375 * 527 94.3 88.4 87.4 102.2 14 17 23 0 12 511 535 385 * 519 98.2 104.1 86.0 89.4 13 526 94.0 15 534 114.9 19 403 94.5 0 12 522 96.3 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 12 197 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------------------------------------- MALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 1-15 N MEAN S.D. 15-29 N MEAN S.D. 29-57 N MEAN S.D. 57-105 N MEAN S.D. -105 N MEAN S.D. 55 306 39.7 55 94 23.8 49 110 35.4 14 48 137.3 14 540 185.4 49 314 38.5 49 94 26.2 48 106 70.9 22 -11 144.4 22 524 172.2 50 281 * 37.6 50 78 * 23.9 48 109 44.1 22 69 89.8 22 512 132.9 59 261 * 33.8 59 71 * 17.0 48 92 38.2 17 30 131.5 17 434 125.5 0 0 0 0 0 38 273 * 38.8 38 74 * 26.4 36 110 45.2 10 122 109.8 10 551 168.2 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 1 198 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ------------------------------------- FEMALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 1-15 N MEAN S.D. 15-29 N MEAN S.D. 29-57 N MEAN S.D. 57-105 N MEAN S.D. -105 N MEAN S.D. 55 145 29.9 55 51 21.6 49 103 47.6 13 91 102.8 13 328 111.6 50 131 23.8 50 51 19.5 49 88 36.1 13 117 58.3 13 363 93.2 50 124 * 23.9 50 48 19.2 49 76 * 42.9 15 105 74.7 15 370 115.5 60 97 * 19.8 58 31 * 12.9 48 54 * 31.1 19 78 79.6 19 237 90.4 0 0 0 0 0 39 101 * 17.0 40 34 * 13.1 40 69 * 27.8 12 157 89.3 11 359 97.8 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 2 199 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 1N 68 MEAN 175 S.D. 15.2 2N 69 MEAN 180 S.D. 13.3 3N 65 MEAN 186 S.D. 13.5 4N 65 MEAN 191 S.D. 15.2 5N 64 MEAN 190 S.D. 15.6 6N 62 MEAN 191 S.D. 16.9 7N 58 MEAN 192 S.D. 17.6 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ---MALE---234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 59 171 15.2 59 177 15.2 58 181 16.2 54 185 15.7 52 188 15.5 54 188 15.4 53 190 17.0 58 172 16.6 57 174 17.5 58 176 * 17.6 50 177 * 14.2 54 180 * 16.8 52 179 * 15.9 51 180 * 14.4 69 170 14.9 68 168 * 14.8 67 169 * 14.0 63 170 * 13.9 65 172 * 14.3 64 170 * 13.2 64 168 * 18.9 68 162 * 15.9 68 154 * 14.3 67 150 * 14.4 63 147 * 14.6 60 147 * 16.2 62 146 * 15.8 59 145 * 15.9 39 170 11.3 39 170 * 14.9 40 171 * 14.1 40 173 * 16.6 39 173 * 16.0 40 170 * 21.1 39 170 * 19.5 38 160 * 13.3 39 151 * 13.2 38 147 * 13.0 40 146 * 17.5 39 145 * 13.9 39 146 * 15.9 38 140 * 24.0 Covance 6329-212 3M T-6316.1 PAGE: 1 200 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------------------------------------- MALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 8N 55 52 53 65 0 38 0 MEAN 191 189 179 * 170 * 171 * S.D. 18.4 15.2 15.1 14.6 25.4 9N 56 52 52 64 0 39 0 MEAN 187 188 178 * 166 * 167 * S.D. 16.5 16.4 17.7 13.5 27.8 10 N 60 54 55 65 0 39 0 MEAN 186 188 178 164 * 166 * S.D. 15.4 18.2 16.8 16.9 26.1 11 N 58 54 52 64 0 39 0 MEAN 184 187 177 164 * 169 * S.D. 16.8 16.3 15.5 17.9 25.2 12 N 56 53 55 64 0 38 0 MEAN 181 183 174 162 * 171 * S.D. 17.2 15.8 16.2 19.9 15.2 13 N 58 52 54 63 0 37 0 MEAN 188 186 178 * 165 * 174 * S.D. 20.7 15.6 16.0 18.4 14.3 14 N 52 48 48 59 0 38 0 MEAN 185 184 169 * 168 * 173 * S.D. 17.9 17.6 21.5 15.7 14.6 Covance 6329-212 3M T-6316.1 PAGE: 2 201 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------------------------------------- MALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 15 N 54 49 46 57 0 38 0 MEAN 191 189 176 * 170 * 175 * S.D. 19.1 17.9 16.9 11.5 15.5 16 N 51 49 49 59 0 38 0 MEAN 187 188 178 * 171 * 176 * S.D. 13.1 18.4 17.4 13.1 15.3 20 N 54 49 48 57 0 38 0 MEAN 192 194 184 177 * 181 * S.D. 16.7 17.8 15.9 19.2 17.0 24 N 55 49 50 58 0 38 0 MEAN 190 189 183 177 * 180 * S.D. 18.2 17.3 16.5 14.2 17.7 28 N 54 49 49 59 0 37 0 MEAN 189 176 * 176 * 176 * 178 * S.D. 17.5 24.2 18.1 14.7 16.2 32 N 55 48 50 57 0 35 0 MEAN 193 190 187 181 * 181 * S.D. 17.5 28.1 15.8 14.7 18.1 36 N 55 47 49 59 0 38 MEAN 195 197 188 185 * 187 S.D. 20.7 19.0 16.5 17.0 18.6 0 Covance 6329-212 3M T-6316.1 PAGE: 3 202 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 40 N 54 MEAN 204 S.D. 26.8 44 N 54 MEAN 195 S.D. 20.1 48 N 54 MEAN 194 S.D. 17.6 52 N 54 MEAN 192 S.D. 17.4 56 N 49 MEAN 187 S.D. 18.9 60 N 48 MEAN 189 S.D. 22.1 64 N 46 MEAN 180 S.D. 32.1 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS --- MALE---234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 46 209 16.4 50 203 19.7 59 195 17.8 0 37 0 192 * 30.6 48 50 57 0 37 0 196 188 183 * 184 * 17.3 18.1 18.3 18.9 48 50 58 0 37 196 186 182 * 185 18.9 18.0 16.5 18.0 0 48 49 58 0 36 194 186 180 * 183 24.0 17.4 17.0 18.4 0 48 180 43.8 48 179 28.0 48 180 23.4 0 36 182 25.8 0 45 193 34.1 48 184 28.5 47 174 24.1 0 36 182 39.9 0 43 189 31.8 45 175 29.7 44 169 27.7 0 34 183 30.3 0 Covance 6329-212 3M T-6316.1 PAGE: 4 203 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------------------------------------- MALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 68 N 44 MEAN 182 S.D. 41.1 72 N 40 MEAN 195 S.D. 23.5 76 N 40 MEAN 180 S.D. 45.4 80 N 35 MEAN 194 S.D. 22.4 84 N 32 MEAN 184 S.D. 32.3 N MEAN S.D. 30 171 38.0 92 N 25 MEAN 155 S.D. 58.4 42 191 36.2 41 202 33.9 39 184 42.4 33 184 54.7 29 203 47.6 28 190 29.3 27 179 32.4 45 172 42.6 40 183 35.2 38 177 43.8 33 181 45.5 32 189 26.5 32 170 26.7 29 172 28.9 43 164 46.8 39 185 28.7 37 179 34.4 32 184 28.4 33 170 44.6 31 166 28.6 29 164 35.5 0 33 192 20.6 0 33 195 26.5 0 32 187 31.2 0 30 184 46.3 0 24 178 40.3 0 21 169 57.7 0 17 160 40.4 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 5 CO CO 204 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 96 N MEAN S.D. 100 N MEAN S.D. 104 N MEAN S.D. 20 159 47.2 14 166 41.4 10 177 17.1 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS --- MALE---234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 25 180 28.7 22 162 45.5 19 149 43.3 25 154 43.6 24 160 28.4 22 155 30.7 24 154 31.3 21 154 25.0 14 163 14.9 0 14 157 42.1 09 168 31.3 08 165 17.9 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 6 205 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 1N 63 MEAN 130 S.D. 23.8 2N 61 MEAN 132 S.D. 20.7 3N 57 MEAN 136 S.D. 15.5 4N 55 MEAN 141 S.D. 15.8 5N 55 MEAN 142 S.D. 18.7 6N 55 MEAN 138 S.D. 15.8 7N 53 MEAN 140 S.D. 17.6 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS --FEMALE--234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 56 124 15.2 55 130 15.9 53 135 22.4 42 138 23.0 46 134 15.5 42 138 18.7 44 133 14.9 54 125 16.0 50 127 16.6 49 129 16.7 46 134 15.2 50 136 20.3 45 135 19.6 50 130 * 16.9 59 122 * 13.2 60 125 16.9 57 124 * 14.9 57 127 * 15.0 56 122 * 12.0 59 121 * 11.5 61 117 * 10.8 57 112 * 14.9 63 105 * 15.9 62 99 * 14.3 55 96 * 12.5 59 97 * 16.0 58 96 * 12.6 60 95 * 11.2 36 121 * 16.0 39 122 * 17.8 40 122 * 14.8 35 121 * 13.2 40 122 * 13.8 39 117 * 13.4 40 116 * 16.2 33 115 * 14.3 35 111 * 16.5 36 101 * 12.3 36 100 * 13.1 35 98 * 10.3 36 98 * 14.6 38 98 * 16.0 Covance 6329-212 3M T-6316.1 PAGE: 7 206 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ------------------------------------- FEMALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 8N 52 45 44 57 0 33 0 MEAN 135 140 136 119 * 120 * S.D. 16.3 23.7 25.0 12.0 16.0 9N 54 47 48 60 0 39 0 MEAN 131 128 127 118 * 114 * S.D. 14.4 19.2 17.3 23.4 13.7 10 N 51 44 47 60 0 37 0 MEAN 135 130 127 115 * 114 * S.D. 21.5 19.6 18.4 15.6 10.4 11 N 52 44 49 62 0 37 0 MEAN 131 128 131 115 * 116 * S.D. 18.9 22.2 25.6 20.1 13.6 12 N 54 43 51 62 0 38 0 MEAN 127 121 128 113 * 114 * S.D. 13.6 13.7 16.5 14.4 16.9 13 N 56 47 54 64 0 39 0 MEAN 124 125 125 113 * 111 * S.D. 15.9 21.8 20.3 20.9 14.0 14 N 52 45 45 57 0 38 0 MEAN 127 126 124 113 * 112 * S.D. 17.8 22.9 15.6 19.5 11.7 Covance 6329-212 3M T-6316.1 PAGE: 8 207 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ------------------------------------- FEMALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 15 N 48 38 42 55 0 39 0 MEAN 127 125 128 114 * 114 * S.D. 13.4 21.6 16.1 10.8 12.0 16 N 49 42 44 56 0 38 0 MEAN 127 127 127 113 * 114 * S.D. 13.6 16.1 19.4 10.5 13.4 20 N 48 41 47 53 0 38 0 MEAN 134 132 134 120 * 121 * S.D. 15.4 19.6 21.0 12.0 13.1 24 N 53 47 47 54 0 38 0 MEAN 134 140 135 123 * 122 * S.D. 17.1 24.0 20.1 18.1 12.4 28 N 51 46 45 55 0 39 0 MEAN 137 136 133 122 * 125 * S.D. 19.3 19.0 14.6 15.1 13.5 32 N 54 48 48 55 0 36 0 MEAN 140 143 141 130 * 129 * S.D. 20.7 20.6 21.4 18.0 12.0 36 N 54 47 49 54 0 37 0 MEAN 146 149 142 135 * 134 * S.D. 23.1 25.0 19.5 27.0 12.8 Covance 6329-212 3M T-6316.1 PAGE: 9 208 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ------------------------------------- FEMALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 40 N 51 MEAN 158 S.D. 21.8 48 162 21.1 49 158 22.4 56 148 24.9 0 38 149 15.2 0 44 N 54 48 50 56 0 40 0 MEAN 154 149 150 133 * 137 * S.D. 24.0 20.3 19.6 20.4 20.3 48 N 53 49 50 56 0 39 0 MEAN 147 145 142 137 * 135 * S.D. 22.6 19.3 19.9 26.5 14.8 52 N 54 46 48 55 0 39 0 MEAN 148 144 137 * 133 * 130 * S.D. 24.8 15.7 25.2 20.8 15.6 56 N 48 MEAN 145 S.D. 29.9 47 138 17.8 47 134 26.7 47 130 * 23.4 0 39 142 17.5 0 60 N 46 MEAN 136 S.D. 35.9 46 144 17.9 45 136 21.4 44 132 19.8 0 36 148 21.9 0 64 N 45 MEAN 142 S.D. 30.7 46 136 34.6 45 136 19.1 43 128 17.7 0 36 141 20.7 0 Covance 6329-212 3M T-6316.1 PAGE: 10 209 CO CO SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 68 N 44 MEAN 147 S.D. 32.0 72 N 44 MEAN 139 S.D. 43.0 76 N 36 MEAN 160 S.D. 37.8 80 N 30 MEAN 164 S.D. 27.2 84 N 28 MEAN 154 S.D. 39.8 N MEAN S.D. 26 143 35.3 92 N 20 MEAN 137 S.D. 33.8 Table 9 Summary of Food Consumption Data (g) 104 -WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ---FEMALE-234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 40 148 19.7 44 141 30.9 45 133 32.9 0 37 146 36.5 0 40 150 25.7 38 145 23.5 39 134 19.9 0 34 159 * 24.2 0 33 155 25.3 33 146 26.4 37 136 * 29.5 0 31 153 34.9 0 28 32 32 0 29 153 147 137 * 154 38.6 30.3 28.6 33.0 0 28 151 33.2 29 143 35.6 31 132 29.8 0 26 147 30.4 0 25 140 27.0 26 135 44.6 27 125 23.6 0 20 153 31.5 0 24 127 48.7 23 139 27.7 29 125 35.3 0 20 133 39.2 0 210 Covance 6329-212 3M T-6316.1 PAGE: 11 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ------------------------------------- FEMALE GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 96 N MEAN S.D. 100 N MEAN S.D. 104 N MEAN S.D. 16 144 33.5 14 133 27.2 13 128 48.8 20 126 37.8 14 128 30.6 13 136 10.7 22 134 23.6 17 128 26.7 15 127 30.7 24 114 * 39.0 23 128 35.9 18 124 31.9 0 14 151 34.8 0 12 144 35.5 0 12 152 26.2 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 12 211 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 1N 59 MEAN 0.259 S.D. 0.016 2N 59 MEAN 0.231 S.D. 0.012 3N 58 MEAN 0.210 S.D. 0.011 4N 54 MEAN 0.197 S.D. 0.011 5N 52 MEAN 0.187 S.D. 0.009 6N 54 MEAN 0.177 S.D. 0.008 7N 53 MEAN 0.172 S.D. 0.011 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM -- MALE---5 300 PPM 6 100 PPM 7 300 PPM 58 2.618 0.170 57 2.323 0.166 58 2.088 0.123 50 1.953 0.108 54 1.852 0.115 52 1.737 0.102 51 1.694 0.091 69 8.724 0.561 68 7.544 0.467 67 6.864 0.306 63 6.393 0.280 65 6.067 0.300 64 5.698 0.236 64 5.418 0.409 68 25.408 1.919 68 21.735 1.585 67 19.533 1.195 62 18.112 0.938 59 17.253 0.987 62 16.576 0.999 59 16.058 0.918 39 8.873 0.572 39 7.707 0.503 40 6.988 0.339 40 6.533 0.338 39 6.135 0.341 40 5.764 0.435 39 5.536 0.320 38 25.193 1.230 39 21.759 1.462 38 19.559 0.920 40 18.294 1.663 39 17.415 0.733 39 16.815 1.021 38 15.803 2.209 Covance 6329-212 3M T-6316.1 PAGE: 1 212 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 8N MEAN S.D. 9N MEAN S.D. 10 N MEAN S.D. 11 N MEAN S.D. 12 N MEAN S.D. 13 N MEAN S.D. 14 N MEAN S.D. 52 0.164 0.008 52 0.156 0.010 54 0.152 0.011 54 0.147 0.009 53 0.141 0.009 52 0.143 0.010 48 0.139 0.010 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM -- MALE--5 300 PPM 6 100 PPM 7 300 PPM 53 1.629 0.099 52 1.564 0.104 55 1.517 0.105 52 1.472 0.086 55 1.421 0.101 54 1.426 0.097 48 1.357 0.153 65 5.329 0.251 64 5.031 0.280 65 4.871 0.355 64 4.772 0.324 64 4.616 0.364 63 4.616 0.346 59 4.655 0.348 0 0 0 0 0 0 0 38 5.325 0.496 39 5.043 0.592 39 4.940 0.477 39 4.926 0.349 38 4.800 0.279 37 4.808 0.263 38 4.709 0.241 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 2 213 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 15 N MEAN S.D. 16 N MEAN S.D. 20 N MEAN S.D. 24 N MEAN S.D. 28 N MEAN S.D. 32 N MEAN S.D. 36 N MEAN S.D. 49 0.140 0.009 49 0.137 0.009 49 0.134 0.008 49 0.126 0.008 49 0.114 0.018 48 0.120 0.016 47 0.120 0.008 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM -- MALE--5 300 PPM 6 100 PPM 7 300 PPM 46 1.397 0.115 49 1.384 0.105 48 1.367 0.087 50 1.309 0.090 49 1.231 0.116 50 1.276 0.093 49 1.261 0.102 57 4.641 0.222 59 4.608 0.248 57 4.545 0.340 58 4.447 0.291 59 4.300 0.275 57 4.298 0.303 59 4.355 0.428 0 0 0 0 0 0 0 38 4.694 0.241 38 4.661 0.227 38 4.586 0.264 38 4.438 0.245 37 4.249 0.246 35 4.246 0.250 38 4.305 0.255 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 3 214 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 40 N MEAN S.D. 44 N MEAN S.D. 48 N MEAN S.D. 52 N MEAN S.D. 56 N MEAN S.D. 60 N MEAN S.D. 64 N MEAN S.D. 46 0.126 0.009 48 0.115 0.007 48 0.111 0.010 48 0.108 0.012 48 0.099 0.025 45 0.104 0.025 43 0.101 0.022 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM -- MALE--5 300 PPM 6 100 PPM 7 300 PPM 50 1.340 0.110 50 1.203 0.094 50 1.155 0.095 49 1.126 0.084 48 1.066 0.173 48 1.071 0.164 45 1.008 0.153 59 4.532 0.407 57 4.103 0.346 58 3.994 0.321 58 3.847 0.332 48 3.786 0.474 47 3.629 0.481 44 3.477 0.492 0 0 0 0 0 0 0 37 4.358 0.631 37 4.069 0.229 37 3.996 0.268 36 3.841 0.222 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 4 215 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 68 N MEAN S.D. 72 N MEAN S.D. 76 N MEAN S.D. 80 N MEAN S.D. 84 N MEAN S.D. N MEAN S.D. 92 N MEAN S.D. 42 0.100 0.017 41 0.107 0.034 39 0.095 0.020 33 0.095 0.027 29 0.103 0.025 28 0.098 0.021 27 0.092 0.013 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM -- MALE--5 300 PPM 6 100 PPM 7 300 PPM 45 43 0 0 0 0.997 3.343 0.225 0.831 40 39 0 0 0 1.035 3.705 0.181 0.430 38 37 0 0 0 1.004 3.529 0.226 0.548 33 32 0 0 0 1.010 3.626 0.239 0.466 32 33 0 0 0 1.057 3.310 0.143 0.778 32 31 0 0 0 0.957 3.285 0.118 0.544 29 29 0 0 0 0.952 3.204 0.122 0.709 Covance 6329-212 3M T-6316.1 PAGE: 5 CO CO 216 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 96 N MEAN S.D. 100 N MEAN S.D. 104 N MEAN S.D. 25 0.091 0.013 22 0.085 0.020 19 0.081 0.019 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM ---- MALE--5 300 PPM 6 100 PPM 7 300 PPM 25 24 0 0 0 0.860 3.065 0.210 0.600 24 21 0 0 0 0.901 3.159 0.134 0.542 22 14 0 0 0 0.874 3.360 0.142 0.664 Covance 6329-212 3M T-6316.1 PAGE: 6 217 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 1N 56 MEAN 0.307 S.D. 0.037 2N 55 MEAN 0.286 S.D. 0.033 3N 53 MEAN 0.271 S.D. 0.051 4N 42 MEAN 0.256 S.D. 0.038 5N 46 MEAN 0.239 S.D. 0.023 6N 42 MEAN 0.234 S.D. 0.027 7N 44 MEAN 0.219 S.D. 0.018 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM --FEMALE--5 300 PPM 6 100 PPM 7 300 PPM 54 3.070 0.386 50 2.791 0.319 49 2.595 0.317 46 2.540 0.238 50 2.442 0.271 45 2.312 0.271 50 2.183 0.225 59 10.166 1.080 60 9.372 1.179 57 8.509 0.873 57 8.314 0.912 56 7.581 0.610 59 7.316 0.624 61 6.942 0.555 57 28.712 3.562 63 25.653 4.087 62 22.650 3.249 55 21.385 2.132 59 20.872 2.782 58 20.229 2.277 60 19.568 1.815 36 10.135 1.157 39 9.093 1.058 40 8.376 0.800 35 7.946 0.574 40 7.644 0.593 39 7.115 0.638 40 6.839 0.727 33 29.270 3.365 35 26.381 4.036 36 22.505 2.640 36 21.593 2.667 35 20.702 1.771 36 20.300 3.150 38 20.004 3.757 Covance 6329-212 3M T-6316.1 PAGE: 7 218 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 8N MEAN S.D. 9N MEAN S.D. 10 N MEAN S.D. 11 N MEAN S.D. 12 N MEAN S.D. 13 N MEAN S.D. 14 N MEAN S.D. 45 0.225 0.040 47 0.201 0.029 44 0.199 0.027 44 0.193 0.035 43 0.181 0.023 47 0.184 0.031 45 0.182 0.031 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM --FEMALE-5 300 PPM 6 100 PPM 7 300 PPM 44 2.237 0.390 48 2.007 0.203 47 1.993 0.228 49 2.016 0.342 51 1.952 0.246 54 1.879 0.275 45 1.846 0.155 57 6.907 0.675 60 6.656 1.398 60 6.413 0.760 62 6.456 1.352 62 6.213 0.720 64 6.200 1.400 57 6.174 1.263 0 0 0 0 0 0 0 33 7.017 0.722 39 6.458 0.446 37 6.488 0.543 37 6.508 0.898 38 6.312 0.861 39 6.045 0.705 38 6.058 0.429 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 8 219 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 15 N MEAN S.D. 16 N MEAN S.D. 20 N MEAN S.D. 24 N MEAN S.D. 28 N MEAN S.D. 32 N MEAN S.D. 36 N MEAN S.D. 38 0.179 0.028 42 0.177 0.018 41 0.178 0.027 47 0.183 0.036 46 0.170 0.025 48 0.172 0.028 47 0.175 0.031 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM --FEMALE-5 300 PPM 6 100 PPM 7 300 PPM 42 1.873 0.178 44 1.841 0.217 47 1.844 0.184 47 1.782 0.192 45 1.696 0.120 48 1.739 0.207 49 1.732 0.205 55 6.130 0.515 56 6.010 0.423 53 6.163 0.484 54 6.214 1.082 55 5.916 0.575 55 6.162 0.808 54 6.349 1.458 0 0 0 0 0 0 0 39 6.128 0.383 38 6.018 0.475 38 6.206 0.436 38 6.030 0.348 39 5.977 0.413 36 6.080 0.475 37 6.125 0.448 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 9 220 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 40 N MEAN S.D. 44 N MEAN S.D. 48 N MEAN S.D. 52 N MEAN S.D. 56 N MEAN S.D. 60 N MEAN S.D. 64 N MEAN S.D. 48 0.184 0.027 48 0.163 0.020 49 0.153 0.018 46 0.147 0.018 47 0.138 0.020 46 0.139 0.018 46 0.129 0.034 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM --FEMALE-5 300 PPM 6 100 PPM 7 300 PPM 49 1.853 0.258 50 1.677 0.195 50 1.550 0.215 48 1.454 0.250 47 1.390 0.215 45 1.394 0.203 45 1.371 0.209 56 6.788 1.158 56 5.946 0.888 56 5.976 1.169 55 5.686 0.899 47 5.395 0.844 44 5.267 0.531 43 4.995 0.636 0 0 0 0 0 0 0 38 6.631 0.448 40 5.912 0.783 39 5.715 0.425 39 5.355 0.552 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 10 221 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 68 N MEAN S.D. 72 N MEAN S.D. 76 N MEAN S.D. 80 N MEAN S.D. 84 N MEAN S.D. N MEAN S.D. 92 N MEAN S.D. 40 0.137 0.019 40 0.136 0.026 33 0.137 0.023 28 0.131 0.034 28 0.127 0.026 25 0.119 0.020 24 0.109 0.037 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM --FEMALE-5 300 PPM 6 100 PPM 7 300 PPM 44 45 0 0 0 1.409 5.151 0.226 1.316 38 39 0 0 0 1.402 5.016 0.199 0.468 33 37 0 0 0 1.353 5.178 0.217 1.054 32 32 0 0 0 1.350 5.107 0.238 0.672 29 31 0 0 0 1.276 4.949 0.288 0.868 26 27 0 0 0 1.179 4.659 0.386 0.788 23 29 0 0 0 1.205 4.674 0.224 1.483 Covance 6329-212 3M T-6316.1 PAGE: 11 CO CO 222 SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 96 N MEAN S.D. 100 N MEAN S.D. 104 N MEAN S.D. 20 0.109 0.031 14 0.109 0.027 13 0.114 0.022 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 3 30 PPM 4 100 PPM --- FEMALE-5 300 PPM 6 100 PPM 7 300 PPM 22 24 0 0 0 1.125 4.213 0.198 1.173 17 23 0 0 0 1.038 4.860 0.212 1.582 15 18 0 0 0 1.029 4.440 0.212 1.039 Covance 6329-212 3M T-6316.1 PAGE: 12 223 Table 11 Summary of Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC X106/iL HGB G/DL HCT % MCV FL MCH MCHC PLT PG % X103/iL 0 MEAN S.D. N 300 MEAN S.D. N 8.27 .267 5 15.3 .44 5 44.7 1.43 5 54.1 1.10 5 6.61 * .609 5 12.7 * .68 5 37.1 * 2.14 5 56.3 2.65 5 18.5 .40 5 19.2 .82 5 34.1 .61 5 34.1 .72 5 1269 111.0 5 1092 * 112.7 5 Covance 6329-212 3M T-6316.1 224 DOSE ppm WBC X103/^L Table 11 Summary of Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/m L MONO EOSIN X103/m L X103/m L BASO N-SEG% X103/m L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% 0 MEAN 8.5 1.2 6.9 .4 .1 .0 15 78 6 1 0 S.D. 3.27 .41 3.52 .29 .04 .00 6.1 8.8 4.0 .5 .0 N 55555555555 300 MEAN 6.5 1.0 5.2 .3 .0 * .0 17 78 4 1 0 S.D. 1.98 .29 2.10 .19 .05 .00 11.7 12.1 2.9 .4 .0 N 55555555555 225 Table 11 Summary of Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC X106/ L HGB G/DL HCT % MCV FL MCH MCHC PLT PG % X103/^L 0 MEAN S.D. N 300 MEAN S.D. N 7.83 .321 5 14.7 .40 5 43.6 1.62 5 55.6 1.13 5 6.83 * .388 5 13.0 * .81 5 37.9 * 2.43 5 55.5 1.31 5 18.8 .56 5 19.0 .46 5 33.8 .48 5 34.2 .29 5 1114 88.8 5 1110 113.7 5 Covance 6329-212 3M T-6316.1 226 DOSE ppm WBC X103/^L Table 11 Summary of Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/m L MONO EOSIN X103/m L X103/m L BASO N-SEG% X103/m L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% 0 MEAN 7.7 .7 6.6 .3 .1 .0 10 84 4 2 0 S.D. 2.85 .31 2.95 .11 .09 .00 6.5 9.1 2.2 1.7 .0 N 55555555555 300 MEAN 5.8 .6 5.0 .2 .0 * .0 10 86 3 1 0 S.D. .78 .30 .93 .10 .00 .00 6.4 5.7 1.1 .4 .0 N 55555555555 227 Table 12 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106//2L G/DL FL PG X103//2L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 8.54 .403 10 8.72 .268 10 8.33 .455 10 8.18 .347 10 15.3 .48 10 15.6 .45 10 15.0 .71 10 15.0 .43 10 45.7 1.34 10 46.6 1.17 10 44.6 1.87 10 44.4 1.57 10 53.6 1.46 10 53.5 1.21 10 53.6 1.45 10 54.4 1.23 10 17.9 .55 10 17.9 .54 10 18.0 .38 10 18.4 .51 10 33.5 .53 10 33.5 .47 10 33.7 .37 10 33.8 .42 10 1240 156.0 10 1140 67.8 10 1143 158.3 10 1141 80.6 10 Covance 6329-212 3M T-6316.1 228 DOSE ppm WBC X103/m L Table 12 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/m L MONO EOSIN BASO N-SEG% X103/m L X103/m L X103/m L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% 0 MEAN 7.4 1.2 5.3 .7 .1 .0 17 71 10 2 0 S.D. 1.31 .38 1.27 .19 .04 .00 5.2 7.1 2.8 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 10.4 1.5 7.9 .8 .2 .0 15 75 7 2 0 S.D. 3.31 .54 2.79 .29 .11 .00 4.6 6.6 2.3 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 9.2 1.3 7.0 .7 .2 .0 15 75 8 2 0 S.D. 1.85 .41 1.59 .28 .07 .00 4.6 4.7 2.4 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 8.8 1.3 6.7 .7 .1 .0 15 76 8 1 0 S.D. 2.64 .39 2.23 .24 .05 .00 2.6 3.3 2.5 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 229 Table 12 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/^L G/DL % FL PG % X103/iL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 8.15 .469 10 8.12 .411 10 8.01 .419 10 7.79 .258 9 15.5 .65 10 15.3 .64 10 15.2 .55 10 14.9 .23 9 45.9 2.09 10 45.3 2.16 10 45.5 1.63 10 43.7 .82 9 56.4 1.17 10 55.8 1.97 10 56.8 1.72 10 56.2 1.46 9 19.0 .61 10 18.8 .67 10 19.1 .80 10 19.2 .61 9 33.7 .46 10 33.7 .38 10 33.6 .45 10 34.1 .54 9 1040 113.7 10 1000 222.8 10 1054 124.0 10 1023 56.2 9 Covance 6329-212 3M T-6316.1 230 DOSE ppm WBC X103/m L Table 12 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/^L MONO EOSIN BASO N-SEG% X103/ L X103/m L X103/ L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% 0 MEAN 6.4 .8 5.1 .4 .1 .0 13 79 6 2 0 S.D. 1.63 .22 1.44 .13 .04 .00 3.6 4.2 1.4 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 6.9 .8 5.5 .4 .1 .0 11 81 6 2 0 S.D. 1.77 .43 1.48 .12 .03 .00 4.5 4.8 2.0 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 7.5 .8 6.3 .4 .1 .0 12 80 5 2 0 S.D. 5.43 .22 5.06 .19 .09 .00 5.5 5.9 1.8 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 6.2 .6 5.1 .4 .1 .0 11 80 6 2 0 S.D. 1.80 .26 1.68 .12 .06 .00 4.8 6.6 3.0 .7 .0 N 99999999999 231 Table 13 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/^L G/DL % FL PG % X103/m L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 8.33 .445 10 8.19 .879 10 8.27 .374 10 8.22 .398 10 14.8 .47 10 14.6 1.46 10 14.9 .51 10 14.5 .59 10 44.2 1.41 10 44.0 4.15 10 44.0 1.12 10 42.9 2.12 10 53.1 1.63 10 53.8 1.83 10 53.2 1.98 10 52.2 2.24 10 17.8 .66 10 17.9 .66 10 18.0 .78 10 17.6 .77 10 33.4 .40 10 33.3 .45 10 33.8 .44 10 33.7 .44 10 1270 107.3 10 1096 * 100.5 10 1190 131.4 10 1206 55.6 10 Covance 6329-212 3M T-6316.1 232 DOSE ppm WBC X103/m L Table 13 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/m L MONO EOSIN BASO X103/ L X103/m L X103/ L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% 0 MEAN 7.9 1.4 5.5 .8 .2 .0 19 69 10 2 0 S.D. 1.77 .34 1.52 .19 .06 .00 4.2 4.7 1.8 .4 .0 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 7.9 1.4 5.6 .7 .2 .0 17 71 92 0 S.D. 1.62 .65 1.14 .23 .08 .00 4.5 5.3 4.1 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 8.8 2.0 5.8 .8 .2 .0 22 68 92 0 S.D. 1.91 .98 1.22 .27 .07 .00 8.3 8.7 2.2 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 8.9 2.4 5.6 .8 .1 .0 24 65 92 0 S.D. 3.06 2.34 1.27 .28 .04 .00 11.6 11.7 2.4 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 233 Table 13 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/^L G/DL % FL PG % X103/iL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 7.93 .316 10 15.3 .29 10 45.1 1.04 10 56.9 1.76 10 7.54 * .243 10 14.7 * .50 10 43.2 * 1.23 10 57.4 1.88 10 7.78 .396 10 14.9 .46 10 43.9 1.38 10 56.4 1.99 10 7.25 * .268 10 14.1 * .61 10 41.0 * 1.82 10 56.5 1.74 10 19.3 .76 10 19.5 .82 10 19.1 .80 10 19.4 .51 10 33.9 .38 10 34.0 .49 10 33.9 .49 10 34.4 .56 10 1076 96 10 987 176 10 968 94 10 996 103 10 Covance 6329-212 3M T-6316.1 234 DOSE ppm WBC X103/m L Table 13 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/m L MONO EOSIN BASO X103/m L X103/m L X103/m L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T6316.1 BASO% 0 MEAN 5.1 .7 3.7 .6 .1 .0 14 71 12 2 0 S.D. 1.29 .17 1.39 .21 .00 .03 5.2 10.3 5.5 .6 .7 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 5.2 .7 3.9 .5 .1 .0 14 74 10 2 0 S.D. 1.85 .24 1.55 .17 .03 .00 2.8 4.0 3.2 .9 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 4.8 .8 3.3 .5 .1 .0 18 69 11 2 0 S.D. 1.20 .22 .98 .21 .03 .00 4.6 7.5 4.1 .7 .4 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 4.9 .8 3.5 .4 .1 .0 17 72 8 2 0 S.D. .69 .28 .73 .07 .03 .00 5.7 6.7 2.2 .5 .4 N 10 10 10 10 10 10 10 10 10 10 10 235 Table 14 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/^L G/DL % FL PG % X103/m L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 8.01 1.004 10 8.42 .498 10 8.39 .585 10 8.45 .454 10 14.5 1.52 10 15.2 .76 10 14.8 1.12 10 14.7 .68 10 43.3 4.50 10 45.3 2.79 10 43.8 3.17 10 43.3 1.73 10 54.2 2.32 10 18.2 .78 10 33.4 .30 10 53.8 1.88 10 18.1 .52 10 33.6 .61 10 52.2 1.82 10 17.6 .80 10 33.8 .56 10 51.3 * 1.06 10 17.4 * .45 10 33.9 .55 10 1238 229.3 10 1210 176.3 10 1199 97.5 10 1129 184.1 10 Covance 6329-212 3M T6316.1 236 DOSE ppm WBC X103/m L Table 14 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/m L MONO EOSIN BASO X103/m L X103/m L X103/m L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T6316.1 BASO% 0 MEAN 8.5 2.4 4.9 1.0 .2 .0 29 58 12 2 0 S.D. 2.14 .88 1.26 .35 .08 .00 5.1 5.4 3.4 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 7.9 1.9 4.9 .9 .1 .0 24 62 11 2 0 S.D. 1.11 .73 .70 .28 .05 .03 6.9 6.6 3.2 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 8.8 2.5 5.5 .7 .2 .0 28 62 8 2 0 S.D. .90 .79 1.01 .43 .07 .00 8.3 8.8 4.6 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 7.5 1.8 4.7 .8 .2 .0 25 62 11 2 0 S.D. 2.02 .53 1.74 .19 .05 .00 7.1 9.5 3.6 .6 .3 N 10 10 10 10 10 10 10 10 10 10 10 237 Table 14 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106//2L G/DL FL PG X103//2L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 7.23 1.388 10 7.42 .589 10 7.44 .979 10 6.92 1.008 10 14.2 1.95 10 14.3 1.03 10 14.6 1.86 10 13.6 1.62 10 41.5 6.15 10 42.4 2.99 10 42.8 5.57 10 39.4 4.74 10 58.2 4.19 10 57.2 1.78 10 57.6 2.13 10 57.1 2.24 10 20.0 1.81 10 19.3 .85 10 19.6 .83 10 19.7 .79 10 34.4 .64 10 33.7 * .56 10 34.0 .51 10 34.6 .43 10 998 125 10 935 125 10 918 133 10 907 80 10 Covance 6329-212 3M T6316.1 238 DOSE ppm WBC X103/m L Table 14 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/m L MONO EOSIN BASO X103/m L X103/m L X103/m L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T6316.1 BASO% 0 MEAN 4.5 1.1 2.7 .6 .1 .0 25 60 13 2 0 S.D. .59 .39 .40 .08 .03 .00 6.8 6.8 1.2 .5 .4 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 4.8 1.5 2.6 .6 .1 .0 26 59 12 2 0 S.D. 1.95 1.60 .74 .34 .03 .03 13.8 14.8 1.9 .9 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 5.7 1.2 3.8 .5 .1 .0 22 66 93 0 S.D. 1.60 .49 1.43 .20 .07 .00 7.4 8.4 3.8 1.4 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 5.8 * 1.6 3.4 .7 .2 .0 28 58 11 3 0 S.D. .74 .33 .63 .19 .08 .00 4.6 5.7 4.0 1.2 .3 N 10 10 10 10 10 10 10 10 10 10 10 239 Table 15 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 300 MEAN S.D. N 7 3.8 5 8 2.5 5 9 1.7 5 6 1.9 5 14 * 3.6 5 Covance 6329-212 3M T-6316.1 240 Table 15 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 300 MEAN S.D. N 3 2.7 5 5 1.6 5 4 2.7 5 5 1.6 5 15 * 7.9 5 Covance 6329-212 3M T-6316.1 241 DOSE ppm Table 16 Summary of Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 106 13 .8 6.7 4.6 2.2 .1 53 S.D. 6.3 1.9 .13 .48 .19 .31 .08 13.3 N 55555555 300 MEAN S.D. N 94 * 18 * .7 7.1 5.5 * 1.6 * .5 * 6.2 2.2 .08 .61 .38 .30 .09 5555555 7* 5.2 5 Covance 6329-212 3M T-6316.1 242 DOSE ppm Table 16 Summary of Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 300 MEAN S.D. N 113 24.8 5 179 * 55.6 5 42 7.0 5 271 * 213.8 5 2 10.5 1.6 .21 55 1 10.4 1.2 .26 55 7.8 .79 5 7.7 .34 5 148 1.5 5 147 1.6 5 5.7 .26 5 5.6 .32 5 103 1.9 5 103 2.2 5 Covance 6329-212 3M T-6316.1 243 Table 16 Summary of Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 300 MEAN S.D. N 9 3.5 5 26 * 5.3 5 Covance 6329-212 3M T-6316.1 244 DOSE ppm Table 16 Summary of Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 109 13 .8 7.3 5.4 1.9 .3 69 S.D. 8.8 2.3 .11 .31 .29 .19 .09 16.2 N 55555555 300 MEAN 86 * 22 * .8 7.7 6.2 * 1.5 .4 11 * S.D. 20.0 3.7 .10 1.00 .63 .40 .08 4.0 N 55555555 Covance 6329-212 3M T-6316.1 245 DOSE ppm Table 16 Summary of Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 300 MEAN S.D. N 129 21.6 5 145 78.4 5 42 19.8 5 94 67.7 5 1 11.1 1.1 .38 55 1 11.2 1.7 .40 55 8.4 1.99 5 8.6 1.22 5 148 2.3 5 149 2.8 5 5.9 .61 5 5.6 .34 5 103 2.6 5 105 1.5 5 Covance 6329-212 3M T-6316.1 246 Table 16 Summary of Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 300 MEAN S.D. N 3 2.7 5 26 * 6.6 5 Covance 6329-212 3M T-6316.1 247 DOSE ppm Table 17 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 111 13 .7 7.4 5.0 2.4 .2 82 S.D. 18.0 1.4 .13 .32 .16 .23 .12 14.4 N 10 10 10 10 10 10 10 10 3 MEAN 101 14 .7 7.1 4.7 * 2.4 .2 61 * S.D. 5.7 2.2 .06 .28 .14 .21 .08 6.8 N 10 10 10 10 10 10 10 10 30 MEAN 102 14 .7 7.1 4.8 2.4 .1 53 * S.D. 8.6 2.0 .14 .36 .18 .31 .10 7.9 N 10 10 10 10 10 10 10 10 100 MEAN 94 * 15 .6 7.2 5.1 2.2 .2 29 * S.D. 7.0 1.6 .05 .31 .25 .25 .08 9.8 N 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 248 DOSE ppm Table 17 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 128 32..7 10 130 27.0 10 116 31.0 10 111 32.3 10 41 6.9 10 42 6.1 10 46 14.2 10 47 11.1 10 0 .4 10 10.8 .37 10 0 .7 10 10.6 .28 10 0 .3 10 10.8 .28 10 0 1.3 10 10.6 .35 10 7.7 .62 10 7.8 .69 10 7.8 .55 10 7.7 .42 10 149 1.7 10 148 1.7 10 148 1.5 10 148 1.7 10 6.5 .41 10 6.2 .28 10 6.1 * .34 10 6.1 * .35 10 105 1.3 10 104 2.4 10 106 2.1 10 106 2.4 10 Covance 6329-212 3M T-6316.1 249 DOSE ppm Table 17 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 106 13 .8 8.3 6.1 2.2 .2 95 S.D. 10.4 1.5 .12 .47 .43 .21 .10 29.6 N 10 10 10 10 10 10 10 10 3 MEAN 108 14 .7 8.0 6.0 2.0 .2 80 S.D. 7.9 2.3 .10 .39 .43 .12 .18 12.9 N 10 10 10 10 10 10 10 10 30 MEAN 97 16 .8 7.8 5.8 2.0 .1 69 * S.D. 11.9 3.8 .16 .53 .37 .24 .13 18.3 N 10 10 10 10 10 10 10 10 100 MEAN 94 * 19 * .7 8.1 6.1 2.0 .2 58 * S.D. 9.9 4.3 .12 .31 .19 .32 .12 22.9 N 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 250 DOSE ppm Table 17 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 110 30.2 10 96 14.8 10 118 16.0 10 87 17.1 10 39 18.5 10 32 6.5 10 34 6.0 10 33 4.7 10 0 1.0 10 11.3 .37 10 0 .5 10 11.4 .38 10 0 .5 10 10.8 * .46 10 1 1.5 10 11.1 .27 10 7.0 1.22 10 7.3 .95 10 6.8 1.00 10 6.8 .74 10 149 2.4 10 148 1.8 10 149 2.2 10 149 2.0 10 6.0 .80 10 6.1 .59 10 5.8 .35 10 5.9 .37 10 106 2.0 10 105 1.2 10 106 1.4 10 106 2.2 10 Covance 6329-212 3M T-6316.1 251 DOSE ppm Table 18 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 106 11 .6 7.4 4.6 2.7 S.D. 11.8 1.2 .13 .31 .25 .13 N 10 10 10 10 10 10 .1 .13 10 3 MEAN 108 13 .7 7.4 4.8 2.6 S.D. 12.1 1.8 .12 .43 .39 .19 N 10 10 10 10 10 10 .2 .11 10 30 MEAN S.D. N 98 13 .7 7.2 4.7 2.5 * .1 6.2 1.7 .10 .51 .39 .20 .10 10 10 10 10 10 10 10 100 MEAN S.D. N 95 * 15 * .6 7.7 5.3 * 2.4 * .1 7.3 1.6 .11 .32 .25 .28 .11 10 10 10 10 10 10 10 96 30.3 10 86 19.8 10 68 * 18.8 10 42 * 16.2 10 Covance 6329-212 3M T-6316.1 252 DOSE ppm Table 18 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 114 28.0 10 119 38.3 10 114 38.7 10 165 192.1 10 40 13.8 10 42 21.2 10 57 31.0 10 94 113.7 10 1 1.3 10 10.8 .38 10 0 .7 10 10.6 .39 10 1 1.0 10 10.6 .34 10 1 .8 10 10.7 .32 10 7.4 .82 10 6.9 .66 10 7.2 .40 10 7.2 .40 10 149 1.9 10 147 2.3 10 147 1.4 10 147 1.4 10 6.7 .48 10 6.2 .48 10 6.3 .37 10 6.3 .35 10 106 1.1 10 105 2.4 10 106 1.2 10 106 1.1 10 Covance 6329-212 3M T-6316.1 253 DOSE ppm Table 18 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 101 15 .8 8.0 5.8 2.2 S.D. 13.4 3.7 .24 .65 .53 .23 N 10 10 10 10 10 10 3 MEAN 103 14 .7 8.2 5.8 2.4 S.D. 7.3 2.2 .11 .54 .57 .28 N 10 10 10 10 10 10 30 MEAN 107 16 .6 8.0 5.8 2.2 S.D. 8.9 3.7 .08 .61 .41 .28 N 10 10 10 10 10 10 100 MEAN S.D. N 92 21 * .8 8.5 6.2 2.4 7.1 7.0 .21 .50 .30 .25 10 10 10 10 10 10 .2 87 .10 14 10 10 .2 104 .14 21 10 10 .2 86 .12 19 10 10 .2 71 .14 20 10 10 Covance 6329-212 3M T-6316.1 254 DOSE ppm Table 18 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 122 30.0 10 102 23.6 10 104 22.1 10 105 42.4 10 44 19.3 10 41 17.7 10 37 10.8 10 47 17.3 10 1 1.1 10 2 4.7 10 2 1.4 10 1 1.3 10 11.0 .41 10 11.0 .41 10 11.0 .53 10 11.2 .24 10 6.0 .44 10 5.8 .76 10 6.1 .92 10 6.2 .63 10 147 2.9 10 147 .9 10 147 1.7 10 146 1.6 10 6.1 .57 10 5.7 .54 10 6.1 .69 10 5.8 .71 10 105 2.0 10 105 1.4 10 107 1.3 10 104 2.1 10 Covance 6329-212 3M T-6316.1 255 DOSE ppm Table 19 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 115 13 .6 7.3 4.4 2.9 S.D. 11.7 1.5 .10 .38 .25 .45 N 10 10 10 10 10 10 .1 146 .10 70.8 10 10 3 MEAN 111 12 .6 7.6 4.7 2.9 S.D. 10.8 1.6 .11 .67 .49 .48 N 10 10 10 10 10 10 .2 119 .13 36.1 10 10 30 MEAN 116 14 .7 7.3 4.7 2.6 .2 92 * S.D. 9.4 2.2 .18 .32 .18 .35 .13 38.3 N 10 10 10 10 10 10 10 10 100 MEAN 113 14 .6 7.7 5.3 * 2.4 * .1 S.D. 13.7 1.0 .11 .35 .30 .27 .09 N 10 10 10 10 10 10 10 49 * 21.4 10 Covance 6329-212 3M T-6316.1 256 DOSE ppm Table 19 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 117 23.7 10 108 22.9 10 105 25.4 10 83 * 16.2 10 40 9.4 10 48 14.4 10 51 19.2 10 68 * 26.5 10 4 3.5 10 3 2.7 10 4 4.5 10 6 4.2 10 11.1 .41 10 11.0 .35 10 10.9 .30 10 10.9 .20 10 6.5 .87 10 6.7 .53 10 6.8 .62 10 6.5 .37 10 150 1.7 10 150 1.6 10 150 1.9 10 148 * 1.6 10 6.4 .36 10 6.5 .52 10 6.5 .42 10 6.3 .45 10 109 2.3 10 108 1.3 10 109 1.6 10 109 2.0 10 Covance 6329-212 3M T-6316.1 257 DOSE ppm Table 19 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 108 13 .7 8.0 5.9 2.1 S.D. 14.0 3.0 .12 .70 .58 .25 N 10 10 10 10 10 10 .3 116 .16 22 10 10 3 MEAN 105 13 .7 8.2 5.8 2.5 S.D. 13.0 1.6 .17 .55 .57 .40 N 10 10 10 10 10 10 .2 119 .19 39 10 10 30 MEAN 93 * 14 .8 8.4 5.9 2.4 .3 94 S.D. 7.8 2.1 .09 .37 .22 .33 .25 23 N 10 10 10 10 10 10 10 10 100 MEAN 103 16 .6 8.5 5.9 2.6 * .1 S.D. 11.3 4.2 .08 .42 .22 .37 .12 N 10 10 10 10 10 10 10 88 23 10 Covance 6329-212 3M T-6316.1 258 DOSE ppm Table 19 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 107 33.7 10 104 27.4 10 125 56.9 10 89 19.9 10 43 16.5 10 48 22.0 10 66 72.0 10 63 26.1 10 2 1.8 10 2 2.8 10 5 6.8 10 4 4.0 10 11.1 .44 10 11.2 .43 10 11.1 .42 10 11.3 .29 10 5.8 .73 10 5.8 .44 10 6.2 .85 10 5.8 .48 10 148 2.2 10 149 1.7 10 150 3.9 10 147 1.9 10 5.6 .51 10 6.1 .45 10 5.9 .56 10 5.7 .40 10 106 3.5 10 108 2.8 10 107 2.4 10 107 2.9 10 Covance 6329-212 3M T-6316.1 259 Table 20 Summary of Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm CHOL MG/DL TRIG MG/DL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 100 MEAN S.D. N 152 65.0 14 144 83.4 22 109 33.4 22 81 * 48.7 17 120 68.7 10 189 119.3 14 199 162.2 22 127 73.2 22 110 111.2 17 173 120.7 10 Covance 6329-212 3M T-6316.1 260 Table 20 Summary of Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm CHOL MG/DL TRIG MG/DL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 100 MEAN S.D. N 116 32.6 13 109 24.9 13 100 22.8 15 108 44.8 19 154 * 53.4 12 166 97.3 13 175 128.4 13 116 97.6 15 90 * 88.3 19 482 469.7 12 Covance 6329-212 3M T-6316.1 261 Table 21 Summary of Clinical Urinalysis Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 300 MEAN S.D. N 17.5 10.64 5 28.5 10.88 5 1.020 .0086 5 1.010 .0047 5 7.0 .35 5 6.9 .22 5 Covance 6329-212 3M T-6316.1 262 Table 21 Summary of Clinical Urinalysis Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 300 MEAN S.D. N 19.9 17.75 5 21.6 12.63 5 1.012 .0102 5 1.010 .0051 5 6.8 .27 5 6.7 .45 5 Covance 6329-212 3M T-6316.1 263 Table 22 Summary of Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 3 MEAN S.D. N MEAN S.D. N 100 MEAN S.D. N 18.7 14.79 10 1.024 .0151 10 25.1 15.47 10 1.015 .0079 10 15.8 9.53 10 1.022 .0109 10 19.9 15.56 10 1.022 .0160 10 6.9 .39 10 6.9 .21 10 6.8 .26 10 6.8 .35 10 Covance 6329-212 3M T-6316.1 264 Table 22 Summary of Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 19.0 13.21 10 22.2 16.26 10 19.2 16.50 10 23.2 16.13 10 1.012 .0082 10 1.012 .0102 10 1.014 .0109 10 1.012 .0086 10 6.7 .26 10 6.8 .26 10 6.6 .39 10 6.6 .16 10 Covance 6329-212 3M T-6316.1 265 Table 23 Summary of Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 3 MEAN S.D. N MEAN S.D. N 100 MEAN S.D. N 15.5 8.17 10 1.024 .0087 10 13.7 5.65 10 1.025 .0090 10 13.7 13.33 10 1.030 .0137 10 15.4 13.36 10 1.025 .0100 10 7.0 .33 10 7.2 .35 10 7.0 .33 10 7.0 .33 10 Covance 6329-212 3M T-6316.1 266 Table 23 Summary of Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 18.1 16.96 10 15.9 15.38 10 13.0 13.35 10 13.2 8.01 10 1.019 .0140 10 1.016 .0100 10 1.020 .0190 10 1.019 .0081 10 6.9 .32 10 6.8 .41 10 6.7 .35 10 6.8 .49 10 Covance 6329-212 3M T-6316.1 267 Table 24 Summary of Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 19.3 15.90 10 13.7 6.72 10 14.7 7.46 10 17.3 12.78 10 1.024 .0092 10 1.029 .0106 10 1.026 .0078 10 1.023 .0110 10 6.8 .26 10 7.0 .33 10 6.8 .35 10 6.8 .34 10 Covance 6329-212 3M T-6316.1 268 Table 24 Summary of Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 13.1 7.81 10 19.3 17.82 10 13.7 12.84 10 17.0 14.38 10 1.018 .0074 10 1.018 .0122 10 1.021 .0101 10 1.021 .0131 10 6.4 .28 10 6.4 .16 10 6.4 .24 10 6.4 .16 10 Covance 6329-212 3M T-6316.1 269 Table 25 Summary of Urine Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 300 MEAN S.D. N 27 13.5 5 20 14.2 5 120 36.4 5 41 * 18.8 5 .37 .135 5 .53 .213 4 1.99 1.394 5 1.10 .403 4 Covance 6329-212 3M T-6316.1 270 Table 25 Summary of Urine Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 300 MEAN S.D. N 16 11.4 5 20 9.9 5 53 47.3 5 42 18.0 5 .15 .074 4 .38 .178 4 .46 .164 4 .76 * .113 4 Covance 6329-212 3M T-6316.1 271 Table 26 Summary of Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 28 15.9 10 22 14.5 10 30 24.4 10 29 28.2 10 155 111.3 10 82 51.3 10 127 88.4 10 128 115.5 10 .35 .159 10 .40 .197 10 .32 .108 10 .31 .071 10 1.70 .469 10 1.40 .521 10 1.44 .405 10 1.35 .335 10 Covance 6329-212 3M T-6316.1 272 Table 26 Summary of Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 19 12.0 10 21 16.5 10 18 11.0 10 20 15.4 10 61 47.7 10 70 73.0 10 73 76.5 10 57 51.3 10 .25 .062 10 .26 .139 10 .22 .132 10 .31 .098 10 .74 .256 10 .66 .214 10 .65 .244 10 .72 .271 10 Covance 6329-212 3M T-6316.1 273 Table 27 Summary of Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 28 15.9 10 34 11.2 10 46 20.5 10 43 25.3 10 102 47.8 10 102 34.0 10 135 76.2 10 114 56.2 10 .37 .227 10 .46 .227 10 .43 .197 10 .46 .186 9 1.30 .281 10 1.26 .274 10 1.12 .266 10 1.26 .413 9 Covance 6329-212 3M T-6316.1 274 Table 27 Summary of Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 23 16.1 10 29 22.9 10 21 10.7 9 27 20.8 10 85 58.0 10 66 43.7 10 52 20.2 9 78 38.4 10 .24 .164 8 .26 .148 9 .24 .133 8 .27 .098 10 .81 .318 8 .61 .173 9 .57 .229 8 .81 .191 10 Covance 6329-212 3M T-6316.1 275 Table 28 Summary of Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 23 9.5 10 27 7.2 10 28 11.2 10 28 12.5 10 110 52.5 10 141 60.8 10 136 61.5 10 116 58.9 10 .28 .077 8 .33 .102 10 .39 .221 10 .38 .142 9 1.50 .296 8 1.62 .273 10 1.63 .462 10 1.45 .335 9 Covance 6329-212 3M T-6316.1 276 Table 28 Summary of Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE PPm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 29 16.8 10 29 23.4 10 24 12.6 10 31 27.4 10 88 40..9 10 92 68.9 10 101 60.4 10 113 82.0 10 .33 .206 10 .26 .086 8 .24 .107 9 .28 .107 10 .91 .240 10 .87 .283 8 .98 .395 9 1.01 .329 10 Covance 6329-212 3M T-6316.1 277 Table 29 Summary of Organ Weight Data Week 4 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /5;WEEKS=ALL DEATH=1;SUBSET=T LIVER SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 350.7 33.3 5 10.8223 1.5753 ORGAN-TOBODY WT (%) 5 3.0744 0.1520 ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 196.1 20.3 ORGAN WEIGHT (g) 5 6.4594 0.4155 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 362.8 32.5 5 10.7856 1.1739 5 2.9699 0.1104 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 209.0 29.8 5 6.7264 0.7320 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 344.4 28.5 5 10.9453 1.5396 5 3.1683 0.2257 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 198.8 17.2 5 6.8324 0.6354 11 ^ 11 ^ 11 11 11 11 OJ 11 to 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 Covance 6329-212 3M T-6316.1 PAGE: 1 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 3.3170 0.3676 5 3.2329 0.1459 5 3.4373 0.1362 278 Table 29 Summary of Organ Weight Data Week 4 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=1;SUBSET=T LIVER SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 2 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: M5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 343.8 36.4 5 12.2371 0.9341 5 310.0 28.3 5 15.7639 1.4058 5 3.5708 0.2090 5 5.0860 0.1240 NUMBER IN GROUP: MEAN: STANDARD DEV: F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 191.3 13.1 5 7.1709 0.5706 5 181.6 15.1 5 9.2338 1.4896 5 3.7577 0.3444 5 5.1048 0.8198 279 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 0.0318 0.0042 ORGAN-TOBODY WT (%) 5 0.0072 0.0013 ORGAN-TOBRAIN WT RATIO 5 0.0146 0.0020 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 257.4 31.4 5 0.0400 0.0077 5 0.0155 0.0018 5 0.0198 0.0036 NUMBER IN GROUP MEAN STANDARD DEV 5 317.3 * 50.6 5 0.0278 0.0056 5 0.0088 0.0018 5 0.0136 0.0029 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.0304 * 0.0043 5 0.0179 0.0021 5 0.0167 0.0023 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 280 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 0.0295 0.0029 ORGAN-TOBODY WT (%) 5 0.0067 0.0011 ORGAN-TOBRAIN WT RATIO 5 0.0136 0.0014 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 257.4 31.4 5 0.0373 0.0060 NUMBER IN GROUP MEAN STANDARD DEV 5 317.3 * 50.6 5 0.0261 0.0064 5 0.0082 0.0013 5 0.0126 0.0024 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.0283 0.0078 Covance 6329-212 3M T-6316.1 PAGE: 2 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0145 0.0014 5 0.0184 0.0029 5 0.0166 0.0043 5 0.0156 0.0045 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 281 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL BRAIN SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 2.1728 0.0240 ORGAN-TOBODY WT (%) 5 0.4934 0.0382 ORGAN-TOBRAIN WT RATIO 5 1.0000 0.0000 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 257.4 31.4 5 2.0222 0.0981 5 0.7919 0.0687 5 1.0000 0.0000 NUMBER IN GROUP MEAN STANDARD DEV 5 317.3 * 50.6 5 2.0479 0.1770 5 0.6519 * 0.0594 5 1.0000 0.0000 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 1.8176 * 0.1064 5 1.0704 * 0.0662 5 1.0000 0.0000 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 282 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 1.4309 0.1350 ORGAN-TOBODY WT (%) 5 0.3240 0.0269 ORGAN-TOBRAIN WT RATIO 5 0.6586 0.0623 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) 5 0.9220 0.1189 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3585 0.0268 5 0.4550 0.0456 1 1 1 1 1 1 1 1 1 LD 1 1 1 1 12 1 NUMBER IN GROUP MEAN: STANDARD DEV: 5 317.3 * 50.6 5 1.4077 0.2404 5 0.4435 * 0.0220 5 0.6856 0.0805 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.7874 * 0.0384 5 0.4640 * 0.0309 5 0.4338 0.0216 283 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 1.4168 0.1080 ORGAN-TOBODY WT (%) 5 0.3207 0.0177 ORGAN-TOBRAIN WT RATIO 5 0.6520 0.0489 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) 5 0.9348 0.1123 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3637 0.0252 5 0.4616 0.0441 NUMBER IN GROUP MEAN: STANDARD DEV: 5 317.3 * 50.6 5 1.4785 0.2950 5 0.4640 * 0.0242 5 0.7191 0.1033 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.7994 * 0.0471 5 0.4710 * 0.0323 5 0.4400 0.0132 1 1 1 1 1 1 1 1 1 LD 1 1 1 1 112 284 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LIVER SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 442.3 31.8 5 11.4244 1.6279 ORGAN-TOBODY WT (%) 5 2.5734 0.2217 ORGAN-TOBRAIN WT RATIO 5 5.2578 0.7478 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) 5 7.2921 0.7864 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 2.8388 0.1198 5 3.6049 0.3306 NUMBER IN GROUP MEAN STANDARD DEV 55 317.3 * 21.6104 * 50.6 3.4376 5 6.8321 * 0.5778 F5 5 NUMBER IN GROUP: 10.5688 * MEAN: 1.4711 STANDARD DEV: 55 170.3 * 11.7394 * 14.1 0.7410 5 6.9106 * 0.3939 5 6.4698 * 0.4478 1 1 1 1 1 1 1 1 11 ID 1 1 1 112 285 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LUNG SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 1.4802 0.1217 ORGAN-TOBODY WT (%) 5 0.3350 0.0213 ORGAN-TOBRAIN WT RATIO 5 0.6809 0.0501 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) 5 1.2546 0.1756 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.4874 0.0348 5 0.6195 0.0726 1 1 1 1 1 1 1 1 1 LD 1 1 1 1 12 1 NUMBER IN GROUP MEAN: STANDARD DEV: 5 317.3 * 50.6 5 1.2530 0.2690 5 0.3928 * 0.0306 5 0.6091 0.0971 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.8541 * 0.0609 5 0.5028 0.0340 5 0.4704 * 0.0313 286 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) 5 0.0663 0.0160 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0255 0.0043 5 0.0326 0.0070 NUMBER IN GROUP MEAN STANDARD DEV 5 317.3 * 50.6 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.0468 * 0.0078 5 0.0276 0.0048 5 0.0258 0.0048 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 287 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) 5 0.0733 0.0184 NUMBER IN GROUP MEAN: STANDARD DEV: 5 317.3 * 50.6 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.0527 0.0085 Covance 6329-212 3M T-6316.1 PAGE: 9 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0282 0.0044 5 0.0361 0.0081 5 0.0311 0.0050 5 0.0291 0.0052 1 1 1 1 1 1 1 1 1 LD 1 1 1 1 112 288 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL SPLEEN SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 0.7326 0.1440 ORGAN-TOBODY WT (%) 5 0.1646 0.0248 ORGAN-TOBRAIN WT RATIO 5 0.3372 0.0666 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 257.4 31.4 5 0.4972 0.0520 5 0.1942 0.0188 5 0.2454 0.0142 NUMBER IN GROUP MEAN STANDARD DEV 5 317.3 * 50.6 5 0.5901 0.1298 5 0.1852 0.0199 5 0.2869 0.0482 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.3599 * 0.0407 5 0.2127 0.0312 5 0.1981 * 0.0193 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 289 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 1.4941 0.1390 ORGAN-TOBODY WT (%) 5 0.3378 0.0185 ORGAN-TOBRAIN WT RATIO 5 0.6875 0.0615 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 257.4 31.4 NUMBER IN GROUP MEAN STANDARD DEV 5 317.3 * 50.6 5 1.7529 * 0.1520 5 0.5580 * 0.0501 F5 5 NUMBER IN GROUP: 0.8581 * MEAN: 0.0633 STANDARD DEV: 5 170.3 * 14.1 Covance 6329-212 3M T-6316.1 PAGE: 11 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 1 1 1 1 1 1 1 1 11 ID 1 1 1 112 290 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 1.5159 0.1642 ORGAN-TOBODY WT (%) 5 0.3424 0.0221 ORGAN-TOBRAIN WT RATIO 5 0.6976 0.0746 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) NUMBER IN GROUP MEAN: STANDARD DEV: 5 317.3 * 50.6 5 1.7399 0.1642 5 0.5535 * 0.0492 F5 5 NUMBER IN GROUP: 0.8511 * MEAN: 0.0601 STANDARD DEV: 5 170.3 * 14.1 Covance 6329-212 3M T-6316.1 PAGE: 12 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 1 1 1 1 1 1 1 1 1 LO 1 1 1 1 112 291 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT THYROID/PAR SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 4 0.0128 0.0026 ORGAN-TOBODY WT (%) 4 0.0028 0.0006 ORGAN-TOBRAIN WT RATIO 4 0. 0059 0. 0012 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 257.4 31.4 5 0.0083 0.0021 NUMBER IN GROUP MEAN STANDARD DEV 5 317.3 * 50.6 4 0.0093 0.0015 4 0.0031 0.0009 4 0. 0047 0. 0012 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 4 0.0076 0.0022 Covance 6329-212 3M T-6316.1 PAGE: 13 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0032 0.0007 5 0.0041 0.0010 4 0.0043 0.0013 4 0.0041 0.0013 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 292 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT THYROID/PR SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 0.0113 0.0049 ORGAN-TOBODY WT (%) 5 0.0025 0.0010 ORGAN-TOBRAIN WT RATIO 5 0.0052 0.0022 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 257.4 31.4 5 0.0074 0.0022 NUMBER IN GROUP MEAN STANDARD DEV 5 317.3 * 50.6 5 0.0102 0.0033 5 0.0032 0.0009 5 0.0050 0.0015 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.0078 0.0024 Covance 6329-212 3M T-6316.1 PAGE: 14 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0029 0.0008 5 0.0037 0.0011 5 0.0046 0.0016 5 0.0043 0.0014 1 1 1 1 1 1 1 1 1 ID 1 1 1 1 12 1 293 Table 30 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL UTERUS SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) 5 0.5992 0.1269 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 317.3 * 50.6 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3 * 14.1 5 0.4823 0.1903 Covance 6329-212 3M T-6316.1 PAGE: 15 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.2334 0.0468 5 0.2958 0.0584 5 0.2821 0.1076 5 0.2633 0.0945 1 1 1 1 1 1 1 1 1 LD 1 1 1 1 112 294 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 0.0373 0.0080 ORGAN-TOBODY WT (%) 5 0.0069 0.0008 ORGAN-TOBRAIN WT RATIO 5 0.0168 0.0035 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.0336 0.0052 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 0.0277 0.0061 5 0.0052 0.0013 5 0.0127 0.0031 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.0360 0.0055 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 0.0295 0.0022 5 0.0055 0.0007 5 0.0129 0.0010 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.0369 0.0077 1 ^ 11 1 1 1 OJ 11 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-212 3M T-6316.1 PAGE: 1 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0119 0.0032 5 0.0169 0.0030 5 0.0142 0.0024 5 0.0185 0.0025 5 0.0141 0.0030 5 0.0186 0.0035 295 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 2 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 0.0303 0.0034 5 0.0065 0.0011 5 0.0139 0.0019 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0322 0.0049 5 0.0137 0.0027 5 0.0173 0.0036 296 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 0.0353 0.0076 ORGAN-TOBODY WT (%) 5 0.0065 0.0009 ORGAN-TOBRAIN WT RATIO 5 0.0159 0.0033 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.0337 0.0071 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 0.0256* 0.0043 5 0.0048 * 0.0009 5 0.0118 0.0023 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.0336 0.0052 Covance 6329-212 3M T-6316.1 PAGE: 3 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0119 0.0034 5 0.0171 0.0042 5 0.0132 0.0016 5 0.0173 0.0024 1 ^ 11 1 1 1 OJ 11 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 0.0273 0.0034 5 0.0051 * 0.0004 5 0.0119 0.0012 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.0345 0.0059 5 0.0131 0.0019 5 0.0174 0.0025 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 11ro 1 1 1 112 297 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 4 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 0.0286 0.0030 5 0.0061 0.0009 5 0.0131 0.0018 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0307 0.0036 5 0.0131 0.0023 5 0.0165 0.0028 298 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 2.2215 0.0445 ORGAN-TOBODY WT (%) 5 0.4182 0.0452 ORGAN-TOBRAIN WT RATIO 5 1.0000 0.0000 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 1.9905 0.0791 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 2.1920 0.0960 5 0.4126 0.0123 5 1.0000 0.0000 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 1.9423 0.1026 Covance 6329-212 3M T-6316.1 PAGE: 5 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.6962 0.1058 5 1.0000 0.0000 5 0.7665 0.0587 5 1.0000 0.0000 1 ^ 11 1 1 1 OJ 11 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 2.2818 0.1054 5 0.4273 0.0430 5 1.0000 0.0000 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 1.9846 0.1121 5 0.7566 0.0628 5 1.0000 0.0000 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 11ro 1 1 1 112 299 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 6 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 2.1861 0.0723 5 0.4642 0.0397 5 1.0000 0.0000 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 1.8793 0.1322 5 0.7961 0.0609 5 1.0000 0.0000 300 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 1.9711 0.1974 ORGAN-TOBODY WT (%) 5 0.3686 0.0233 ORGAN-TOBRAIN WT RATIO 5 0.8874 0.0880 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 1.1328 0.1492 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 1.8955 0.1575 5 0.3570 0.0307 5 0.8661 0.0818 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.9311 0.1262 Covance 6329-212 3M T-6316.1 PAGE: 7 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3911 0.0283 5 0.5688 0.0667 5 0.3658 0.0386 5 0.4782 0.0473 1 ^ 11 1 1 1 OJ 11 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 2.0805 0.2924 5 0.3863 0.0302 5 0.9111 0.1154 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 1.1345 0.1316 5 0.4307 0.0362 5 0.5718 0.0615 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 11ro 1 1 1 112 301 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT KIDNEY SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 8 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 2.1488 0.2660 5 0.4535 * 0.0313 5 0.9817 0.1017 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.9648 0.1536 5 0.4053 0.0302 5 0.5124 0.0648 302 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 2.0585 0.2090 ORGAN-TOBODY WT (%) 5 0.3846 0.0203 ORGAN-TOBRAIN WT RATIO 5 0.9265 0.0904 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 1.1236 0.1700 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 1.9405 0.1411 5 0.3656 0.0297 5 0.8873 0.0831 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.9328 0.1505 Covance 6329-212 3M T-6316.1 PAGE: 9 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3874 0.0341 5 0.5644 0.0797 5 0.3652 0.0354 5 0.4787 0.0596 1 ^ 11 1 1 1 OJ 11 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 2.0643 0.2531 5 0.3835 0.0209 5 0.9041 0.0972 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 1.1634 0.1471 5 0.4418 0.0451 5 0.5865 0.0712 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 11ro 1 1 1 112 303 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 10 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 2.1724 0.2812 5 0.4581 * 0.0303 5 0.9929 0.1136 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.9912 0.1534 5 0.4166 0.0305 5 0.5264 0.0638 304 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 535.9 54.5 5 15.9996 2.1505 ORGAN-TOBODY WT (%) 5 2.9783 0.1425 ORGAN-TOBRAIN WT RATIO 5 7.2008 0.9432 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 8.7487 1.2794 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 15.6102 1.7249 5 2.9345 0.2531 5 7.1245 0.7384 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 7.4501 1.0845 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 18.0657 1.7387 5 3.3764 0.3717 5 7.9217 0.7535 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 8.8944 1.3628 1 ^ 11 1 1 1 OJ 11 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Covance 6329-212 3M T-6316.1 PAGE: 11 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 3.0096 0.1220 5 4.4018 0.6612 5 2.9233 0.2753 5 3.8304 0.4607 5 3.3658 0.3182 5 4.4714 0.5448 305 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 12 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 24.2906 * 3.5145 5 5.1173 * 0.3964 5 NUMBER IN GROUP: 11.1093 * MEAN: 1.5191 STANDARD DEV: 5 236.9 21.2 5 9.7445 1.1880 5 4.1155 * 0.3584 5 5.1846 0.5040 306 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 1.8736 0.1592 ORGAN-TOBODY WT (%) 5 0.3510 0.0280 ORGAN-TOBRAIN WT RATIO 5 0.8428 0.0609 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 1.2020 0.1063 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 1.7104 0.0972 5 0.3226 0.0267 5 0.7811 0.0493 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 1.1997 0.1562 Covance 6329-212 3M T-6316.1 PAGE: 13 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.4182 0.0552 5 0.6048 0.0590 5 0.4698 0.0101 5 0.6163 0.0571 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 1.9907 0.2684 5 0.3701 0.0333 5 0.8707 0.0899 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 1.1830 0.0601 5 0.4513 0.0396 5 0.5968 0.0305 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 11ro 1 1 1 112 307 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 14 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 1.6290 0.1108 5 0.3467 0.0448 5 0.7450 0.0411 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 1.0859 0.0776 5 0.4601 0.0363 5 0.5784 0.0303 308 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.0742 0.0125 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.0523 0.0097 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.0608 0.0201 Covance 6329-212 3M T-6316.1 PAGE: 15 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 0.0263 0.0072 5 0.0374 0.0067 5 0.0206 0.0034 5 0.0269 0.0043 5 0.0230 0.0070 5 0.0304 0.0093 309 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT OVARY SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 16 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0514 0.0017 5 0.0218 0.0016 5 0.0275 0.0022 310 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.0596 0.0166 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.0518 0.0140 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.0623 0.0128 Covance 6329-212 3M T-6316.1 PAGE: 17 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 0.0208 0.0061 5 0.0301 0.0090 5 0.0201 0.0047 5 0.0264 0.0063 5 0.0236 0.0040 5 0.0312 0.0054 311 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N -ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP= 1,2,3,4;WEEKS =ALL DEATH=3,-SUBSET =ALL RIGHT OVARY SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 18 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0541 0.0054 5 0.0229 0.0026 5 0.0289 0.0036 312 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 0.7252 0.1659 ORGAN-TOBODY WT (%) 5 0.1345 0.0257 ORGAN-TOBRAIN WT RATIO 5 0.3266 0.0755 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.4690 0.0560 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 0.8193 0.0229 5 0.1544 0.0079 5 0.3743 0.0186 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.4603 0.0829 Covance 6329-212 3M T-6316.1 PAGE: 19 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.1622 0.0152 5 0.2360 0.0293 5 0.1793 0.0145 5 0.2358 0.0329 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 0.7152 0.1209 5 0.1328 0.0166 5 0.3135 0.0524 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.4512 0.0531 5 0.1711 0.0124 5 0.2272 0.0226 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 11ro 1 1 1 112 313 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 20 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 0.6639 0.0293 5 0.1408 0.0101 5 0.3037 0.0066 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.4064 0.0722 5 0.1730 0.0356 5 0.2170 0.0408 314 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 1.6975 0.1684 ORGAN-TOBODY WT (%) 5 0.3204 0.0570 ORGAN-TOBRAIN WT RATIO 5 0.7645 0.0789 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 1.7368 0.0912 5 0.3270 0.0161 5 0.7924 0.0251 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 1.6528 0.0711 5 0.3100 0.0367 5 0.7254 0.0433 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 Covance 6329-212 3M T-6316.1 PAGE: 21 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 315 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT TESTIS SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 22 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 1.7151 0.1400 5 0.3629 0.0209 5 0.7841 0.0500 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 316 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 1.7042 0.1307 ORGAN-TOBODY WT (%) 5 0.3211 0.0473 ORGAN-TOBRAIN WT RATIO 5 0.7677 0.0648 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 1.7733 0.1140 5 0.3339 0.0204 5 0.8087 0.0315 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 1.6684 0.0976 5 0.3131 0.0402 5 0.7324 0.0561 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 Covance 6329-212 3M T-6316.1 PAGE: 23 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 317 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 24 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 1.7278 0.1665 5 0.3653 0.0253 5 0.7902 0.0681 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 318 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT THYROID/PAR SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 0.0125 0.0029 ORGAN-TOBODY WT (%) 5 0.0023 0.0005 ORGAN-TOBRAIN WT RATIO 5 0.0056 0.0012 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.0105 0.0040 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 0.0114 0.0022 5 0.0021 0.0004 5 0.0052 0.0010 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.0076 0.0025 Covance 6329-212 3M T-6316.1 PAGE: 25 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0036 0.0013 5 0.0053 0.0022 5 0.0029 0.0007 5 0.0039 0.0012 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 0.0132 0.0023 5 0.0025 0.0006 5 0.0058 0.0012 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.0093 0.0025 5 0.0035 0.0008 5 0.0047 0.0012 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 11ro 1 1 1 112 319 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT THYROID/PAR SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 26 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 0.0144 0.0008 5 0.0031 0.0003 5 0.0066 0.0005 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0092 0.0017 5 0.0039 0.0005 5 0.0049 0.0007 320 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT THYROID/PR SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 0.0120 0.0021 ORGAN-TOBODY WT (%) 5 0.0022 0.0003 ORGAN-TOBRAIN WT RATIO 5 0.0054 0.0009 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.0080 0.0026 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 0.0128 0.0041 5 0.0024 0.0008 5 0.0058 0.0017 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.0093 0.0033 Covance 6329-212 3M T-6316.1 PAGE: 27 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0029 0.0011 5 0.0041 0.0014 5 0.0037 0.0014 5 0.0048 0.0017 1 ^1 1 1 1 1 OJ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 0.0138 0.0019 5 0.0026 0.0006 5 0.0061 0.0010 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.0119 0.0042 5 0.0045 0.0013 5 0.0059 0.0019 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 11ro 1 1 1 112 321 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT THYROID/PR SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 28 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 0.0125 0.0014 5 0.0026 0.0002 5 0.0057 0.0006 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0092 0.0021 5 0.0039 0.0009 5 0.0048 0.0008 322 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.6337 0.1022 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.5244 0.0677 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.6754 0.1666 Covance 6329-212 3M T-6316.1 PAGE: 29 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 0.2209 0.0448 5 0.3197 0.0613 5 0.2058 0.0151 5 0.2695 0.0269 5 0.2547 0.0519 5 0.3404 0.0847 323 Table 31 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 30 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.5414 0.0477 5 0.2293 0.0207 5 0.2898 0.0368 324 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 0.0350 0.0031 ORGAN-TOBODY WT (%) 5 0.0049 0.0005 ORGAN-TOBRAIN WT RATIO 5 0.0147 0.0016 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 0.0445 0.0140 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 0.0335 71.5 0.0057 10 0.0053 0.0011 10 0.0141 0.0025 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 0.0375 49.7 0.0061 Covance 6329-212 3M T-6316.1 PAGE: 1 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0112 0.0043 5 0.0202 0.0066 10 0.0125 0.0030 10 0.0174 0.0035 325 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 0.0316 0.0049 ORGAN-TOBODY WT (%) 5 0.0044 0.0006 ORGAN-TOBRAIN WT RATIO 5 0.0132 0.0016 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 0.0409 0.0146 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 0.0324 71.5 0.0090 10 0.0052 0.0016 10 0.0137 0.0039 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 0.0347 49.7 0.0041 Covance 6329-212 3M T-6316.1 PAGE: 2 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0103 0.0044 5 0.0185 0.0067 10 0.0117 0.0025 10 0.0161 0.0024 326 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL BRAIN SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 2.3837 0.1629 ORGAN-TOBODY WT (%) 5 0.3307 0.0180 ORGAN-TOBRAIN WT RATIO 5 1.0000 0.0000 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 406.9 32.2 5 2.2100 0.0852 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 2.3804 71.5 0.1067 10 0.3753 * 0.0300 10 1.0000 0.0000 NUMBER IN GROUP MEAN: STANDARD DEV 10 10 306.3 * 2.1689 49.7 0.1318 Covance 6329-212 3M T-6316.1 PAGE: 3 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.5452 0.0395 5 1.0000 0.0000 10 0.7201 * 0.0873 10 1.0000 0.0000 327 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 2.3249 0.1910 ORGAN-TOBODY WT (%) 5 0.3224 0.0201 ORGAN-TOBRAIN WT RATIO 5 0.9749 0.0261 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 1.3605 0.1412 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 2.4578 71.5 0.3355 10 0.3856 * 0.0421 10 1.0314 0.1225 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 1.4508 49.7 0.2497 Covance 6329-212 3M T-6316.1 PAGE: 4 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3375 0.0587 5 0.6164 0.0694 10 0.4751 * 0.0490 10 0.6686 0.1064 328 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 2.3397 0.1416 ORGAN-TOBODY WT (%) 5 0.3247 0.0166 ORGAN-TOBRAIN WT RATIO 5 0.9821 0.0202 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 1.4036 0.1605 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 2.4429 71.5 0.3646 10 0.3827 * 0.0415 10 1.0246 0.1316 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 1.5208 49.7 0.2577 Covance 6329-212 3M T-6316.1 PAGE: 5 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3486 0.0654 5 0.6372 0.0898 10 0.4992 * 0.0611 10 0.7013 0.1130 329 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LIVER SEX DOSE GROUP M1 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 722.2 58.0 5 20.8522 1.6996 ORGAN-TOBODY WT (%) 5 2.8910 0.1779 ORGAN-TOBRAIN WT RATIO 5 8.7696 0.8214 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 406.9 32.2 5 11.1366 0.5964 5 2.7565 0.3325 5 5.0503 0.4241 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 30.8941 * 71.5 5.4082 10 4.8476 * 0.7573 10 NUMBER IN GROUP 12.9831 * MEAN: 2.2029 STANDARD DEV 10 10 306.3 * 17.2772 * 49.7 3.6046 10 5.6508 * 0.8632 10 7.9473 * 1.5028 330 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LUNG SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 2.1417 0.2399 ORGAN-TOBODY WT (%) 5 0.2979 0.0401 ORGAN-TOBRAIN WT RATIO 5 0.9013 0.1183 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 1.7049 0.1511 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 2.2129 71.5 0.3222 10 0.3472 * 0.0412 10 0.9301 0.1290 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 1.6432 49.7 0.1425 Covance 6329-212 3M T-6316.1 PAGE: 7 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.4211 0.0499 5 0.7742 0.0974 10 0.5458 * 0.0770 10 0.7592 0.0720 331 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 0.0646 0.0121 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0 * 71.5 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 0.0731 49.7 0.0139 Covance 6329-212 3M T-6316.1 PAGE: 8 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0159 0.0031 5 0.0292 0.0055 10 0.0245 * 0.0062 10 0.0338 0.0067 332 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 0.0607 0.0097 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0 * 71.5 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 0.0781 49.7 0.0242 Covance 6329-212 3M T-6316.1 PAGE: 9 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0150 0.0026 5 0.0275 0.0044 10 0.0262 * 0.0096 10 0.0361 0.0114 333 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL SPLEEN SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 0.9636 0.1238 ORGAN-TOBODY WT (%) 5 0.1337 0.0168 ORGAN-TOBRAIN WT RATIO 5 0.4063 0.0660 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 0.6205 0.0408 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 0.9192 71.5 0.3274 10 0.1421 0.0398 10 0.3838 0.1251 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 0.5425 49.7 0.0914 Covance 6329-212 3M T-6316.1 PAGE: 10 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.1531 0.0135 5 0.2808 0.0155 10 0.1792 0.0311 10 0.2507 0.0442 334 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 1.6043 0.1032 ORGAN-TOBODY WT (%) 5 0.2228 0.0164 ORGAN-TOBRAIN WT RATIO 5 0.6747 0.0509 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 1.7040 71.5 0.2865 10 0.2710 0.0609 10 0.7196 0.1362 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3 * 49.7 Covance 6329-212 3M T-6316.1 PAGE: 11 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 335 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 1.6110 0.0997 ORGAN-TOBODY WT (%) 5 0.2237 0.0145 ORGAN-TOBRAIN WT RATIO 5 0.6772 0.0428 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 1.7805 * 71.5 0.1392 10 0.2825 * 0.0442 10 0.7506 0.0799 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3 * 49.7 Covance 6329-212 3M T-6316.1 PAGE: 12 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 336 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT THYROID/PAR SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 0.0151 0.0027 ORGAN-TOBODY WT (%) 5 0.0021 0.0003 ORGAN-TOBRAIN WT RATIO 5 0.0063 0.0011 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 0.0148 0.0045 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 0.0235 * 71.5 0.0038 10 0.0037 * 0.0004 10 NUMBER IN GROUP: 0.0099 * MEAN: 0.0015 STANDARD DEV: 10 10 306.3 * 0.0156 49.7 0.0053 Covance 6329-212 3M T-6316.1 PAGE: 13 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0037 0.0011 5 0.0068 0.0022 10 0.0052 0.0021 10 0.0072 0.0024 337 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT THYROID/PR SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) 5 0.0189 0.0044 ORGAN-TOBODY WT (%) 5 0.0026 0.0004 ORGAN-TOBRAIN WT RATIO 5 0.0079 0.0016 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 0.0167 0.0044 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 639.0 * 0.0250 71.5 0.0061 10 0.0039 * 0.0006 10 0.0105 0.0024 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 0.0160 49.7 0.0064 Covance 6329-212 3M T-6316.1 PAGE: 14 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0041 0.0008 5 0.0076 0.0019 10 0.0054 0.0025 10 0.0074 0.0029 338 Table 32 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL UTERUS SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV: TERMINAL BODY WT (g) 5 722.2 58.0 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) 5 1.1161 0.4939 1 ^ 11 1 1 1 11 1 1 1 1 1 1 1 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0 * 71.5 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 306.3 * 1.1694 49.7 0.2972 Covance 6329-212 3M T-6316.1 PAGE: 15 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.2806 0.1321 5 0.5103 0.2406 10 0.3855 0.0989 10 0.5408 0.1387 339 Table 33 Incidence of Macroscopic Observations Week 4 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,2 ,3,4,5;WEEKS=ALL DEATH=1;SUBSET=T -- N U M BER - O F - ANI M A L S - A F F E ' SEX: -MALE-- FEMALE-- GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4 - -5- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 5 5 ** TOP OF LIST ** LIVER (LI) ....... .................................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 5 5 ** END OF LIST 340 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE ** TOP OF LIST ** GENERAL COMMENT (GC) ........ NO MACROSCOPIC LESIONS BONE MARROW SMEAR TAKEN BONE, FEMUR (FE) -- N U M B E R - O F - A N I M A L S - A F F E C T E D SEX: --MALE-- -FEMALE- GROUP: -1- -5- -1- -5 NUMBER: 5 NUMBER EXAMINED: NUMBER EXAMINED: NOT REMARKABLE: MARROW, FEMUR (FM) .................................. NUMBER EXAMINED: NOT REMARKABLE: MARROW, STERNUM (SE) ................................ NUMBER EXAMINED: NOT REMARKABLE: BONE, STERNUM (SB) .................................. NUMBER EXAMINED: NOT REMARKABLE: EYE (EY) ............................................ NUMBER EXAMINED: NOT REMARKABLE: 341 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- BRAIN (BR) .................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 SPINAL CORD (SC) ............ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LUNG (LU) ................... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 5 5 5 MOTTLED 1000 KIDNEY (KD) ................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LIVER (LI) .................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 0 5 2 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DEPRESSED FOCUS(I)/AREA(S) 0100 05 02 0001 02 02 0100 Covance 6329-212 3M T-6316.1 PAGE: 2 A F F E C T E D -- 342 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- HEART (HT) .................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 MUSCLE, SKELETAL (SM) ....... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NERVE, SCIATIC (SN) ......... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, MESENTERIC (MS) ......... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 SPLEEN (SP) ................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 THYMUS (TH) ................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 4 RED FOCUS(I)/AREA(S) 0001 ESOPHAGUS (ES) .............. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 343 Covance 6329-212 3M T-6316.1 PAGE: 3 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- THYROID (TY) ................ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PARATHYROID (PT) ............ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 TRACHEA (TR) ................ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ADRENAL, CORTEX (AC) ........ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 4 5 5 DIFFUSELY RED 0100 ADRENAL, MEDULLA (MA) ....... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PITUITARY (PI) .............. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 HARDERIAN GLAND (HG) ........ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 344 Covance 6329-212 3M T-6316.1 PAGE: 4 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH =2;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5NUMBER: 5 5 5 5 SALIV GL , MANDIB (SG) ....... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PANCREAS (PA) ............... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 STOMACH, GL (ST) ............ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 3 5 4 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) 0101 0100 STOMACH, NONGL (SU) ......... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 DUODENUM (DU) ............... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 JEJUNUM (JE) ................ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 5 A F F E C T E D -- 345 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5NUMBER: 5 5 5 5 ILEUM (IL) .................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 CECUM (CE) .................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 4 5 5 DIFFUSELY RED 0100 COLON (CO) .................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 RECTUM (RE) ................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 SKIN (SK) ................... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 URINARY BLADDER (UB) ........ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PROSTATE (PR) ............... ....................... NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 Covance 6329-212 3M T-6316.1 PAGE: 6 A F F E C T E D -- 346 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- SEMINAL VESICLES (SV) ....... ....................... NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 EPIDIDYMIDES (EP) ........... ....................... NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 TESTIS (TE) ................. ....................... NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 MAMMARY, FEMALE (MF) ........ ....................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 CERVIX (CV) ................. ....................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 UTERUS (UT) ................. ....................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 4 LUMEN FILLED WITH FLUID 0001 OVARY (OV) .................. ....................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 Covance 6329-212 3M T-6316.1 PAGE: 7 A F F E C T E D -- 347 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- VAGINA (VA) ................. ....................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 LN, MEDIASTINAL (ML) ........ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, MANDIBULAR (MN) ......... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 5 4 5 DIFFUSELY RED 1010 CAVITY, ORAL (OC) ........... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, ANT MES/PANC (AP) ....... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 CAVITY, ABDOM (PC) .......... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 4 5 5 EDEMA 0100 Covance 6329-212 3M T-6316.1 PAGE: 8 A F F E C T E D -- 348 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5NUMBER: 5 5 5 5 CALVARIUM/SKULL (CA) ................................ NUMBER EXAMINED: NOT REMARKABLE: MAMMARY, MALE (MM) .................................. NUMBER EXAMINED: NOT REMARKABLE: LN, LUMBAR/ILIAC (LM) ............................... NUMBER EXAMINED: NOT REMARKABLE: ANUS (AN) ........................................... NUMBER EXAMINED: NOT REMARKABLE: LN, RENAL (RL) ...................................... NUMBER EXAMINED: NOT REMARKABLE: TAIL (TI) NUMBER EXAMINED: NOT REMARKABLE: PENIS (PE) .......................................... NUMBER EXAMINED: NOT REMARKABLE: 349 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5NUMBER: 5 5 5 5 T E D -- LN, INGUINAL (IN) ................................... NUMBER EXAMINED: NOT REMARKABLE: CAVITY, THORACIC (TA) ............................... NUMBER EXAMINED: NOT REMARKABLE: FOOT/FOOTPAD(S) (FP) ................................ NUMBER EXAMINED: NOT REMARKABLE: LN, AXILLARY (AX) ................................... NUMBER EXAMINED: NOT REMARKABLE: LN, TRACHEOBRON (TB) ................................ NUMBER EXAMINED: NOT REMARKABLE: ADIPOSE TISSUE (AT) ................................. NUMBER EXAMINED: NOT REMARKABLE: JOINT, OTHER (JT) ................................... NUMBER EXAMINED: NOT REMARKABLE: 350 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5NUMBER: 5 5 5 5 T E D -- AORTA (AO) .......................................... NUMBER EXAMINED: NOT REMARKABLE: LN, POPLITEAL (PP) .................................. NUMBER EXAMINED: NOT REMARKABLE: SUBCUTANEOUS TIS (SQ) ............................... NUMBER EXAMINED: NOT REMARKABLE: TONGUE (TO) ......................................... NUMBER EXAMINED: NOT REMARKABLE: URETER (UE) ......................................... NUMBER EXAMINED: NOT REMARKABLE: AORTIC ARCH (AA) .................................... NUMBER EXAMINED: NOT REMARKABLE: TESTIS/EPIDIDYM (TP) ................................ NUMBER EXAMINED: NOT REMARKABLE: 351 Table 34 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5NUMBER: 5 5 5 5 T E D -- PINNA (PN) .......................................... NUMBER EXAMINED: NOT REMARKABLE: THYROID/PARATHYR (TT) ............................... NUMBER EXAMINED: NOT REMARKABLE: DIAPHRAGM (DP) ...................................... NUMBER EXAMINED: NOT REMARKABLE: BILE DUCT (BD) ...................................... NUMBER EXAMINED: NOT REMARKABLE: AUDITORY SEB GL (AS) ................................ NUMBER EXAMINED: NOT REMARKABLE: ADRENAL (AD) ........................................ NUMBER EXAMINED: NOT REMARKABLE: ** END OF LIST ** 352 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ** TOP OF LIST ** GENERAL COMMENT (GC) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NO MACROSCOPIC LESIONS BONE MARROW SMEAR TAKEN URINARY BLADDER-SMALL LUMEN 22543424 55555555 00000010 BONE, FEMUR (FE) ................ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 MARROW, FEMUR (FM) .............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 MARROW, STERNUM (SE) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 BONE, STERNUM (SB) .............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 EYE (EY) ........................ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 1 353 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- BRAIN (BR) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SPINAL CORD (SC) ................ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LUNG (LU) ....................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 KIDNEY (KD) ..................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 5 5 5 5 5 5 LARGE PELVIS(ES) 10000000 LIVER (LI) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 5 5 5 5 5 5 MOTTLED 10000000 HEART (HT) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 2 354 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- MUSCLE, SKELETAL (SM) ........... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 NERVE, SCIATIC (SN) ............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, MESENTERIC (MS) ............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SPLEEN (SP) ..................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 THYMUS (TH) ..................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 5 5 5 5 5 5 MOTTLED 10000000 ESOPHAGUS (ES) .................. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 THYROID (TY) .................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 3 355 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- PARATHYROID (PT) ................ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 TRACHEA (TR) .................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ADRENAL, CORTEX (AC) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ADRENAL, MEDULLA (MA) ........... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 PITUITARY (PI) .................. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 HARDERIAN GLAND (HG) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SALIV GL, MANDIB (SG) ........... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 4 356 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- PANCREAS (PA) ................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 STOMACH, GL (ST) ................ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 4 5 5 4 5 3 4 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) 00000010 11001011 STOMACH, NONGL (SU) ............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 DUODENUM (DU) ................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 JEJUNUM (JE) .................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ILEUM (IL) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 5 357 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------ -=- -=- -=- -=- -=- -=- -=- -=- CECUM (CE) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 COLON (CO) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 RECTUM (RE) ..................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SKIN (SK) ....................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 4 5 5 4 5 ALOPECIA-FOCAL RED FOCUS(I)/AREA(S) 00000010 00010000 URINARY BLADDER (UB) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 PROSTATE (PR) ................... ................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 4 5 5 5 0 0 0 0 LARGE 10000000 Covance 6329-212 3M T-6316.1 PAGE: 6 358 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- SEMINAL VESICLES (SV) ........... ................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 4 5 5 5 0 0 0 0 LARGE 10000000 EPIDIDYMIDES (EP) ............... ................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 5 5 0 0 0 0 TESTIS (TE) ..................... ................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 5 5 0 0 0 0 MAMMARY, FEMALE (MF) ............ ................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 5 5 CERVIX (CV) ..................... ................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 5 5 UTERUS (UT) ..................... ................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 4 5 LUMEN FILLED WITH FLUID 00000010 Covance 6329-212 3M T-6316.1 PAGE: 7 359 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- OVARY (OV) ...................... ................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 4 5 5 5 CYST(S) 00001000 VAGINA (VA) ..................... ................... NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 5 5 LN, MEDIASTINAL (ML) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, MANDIBULAR (MN) ............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 3 5 5 5 4 4 5 DIFFUSELY RED RED FOCUS(I)/AREA(S) 12 00001 0 00000100 CAVITY, ORAL (OC) ............... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, ANT MES/PANC (AP) ........... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 8 360 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- CAVITY, ABDOM (PC) .............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 CALVARIUM/SKULL (CA) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 MAMMARY, MALE (MM) .............. ................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 5 5 0 0 0 0 LN, LUMBAR/ILIAC (LM) ........... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ANUS (AN) ....................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, RENAL (RL) .................. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 TAIL (TI) ....................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 9 361 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- PENIS (PE) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, INGUINAL (IN) ............... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 CAVITY, THORACIC (TA) ........... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 FOOT/FOOTPAD(S) (FP) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, AXILLARY (AX) ............... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, TRACHEOBRON (TB) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ADIPOSE TISSUE (AT) ............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 10 T E D -- 362 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- JOINT, OTHER (JT) ............... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 AORTA (AO) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LN, POPLITEAL (PP) .............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 SUBCUTANEOUS TIS (SQ) ........... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 TONGUE (TO) ..................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 URETER (UE) ..................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 AORTIC ARCH (AA) ................ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 11 T E D -- 363 Table 35 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: ---MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- TESTIS/EPIDIDYM (TP) ............ ................... NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 5 5 0 0 0 0 PINNA (PN) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 THYROID/PARATHYR (TT) ........... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 DIAPHRAGM (DP) .................. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 BILE DUCT (BD) .................. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 AUDITORY SEB GL (AS) ............ ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ADRENAL (AD) .................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 ** END OF LIST ** Covance 6329-212 3M T-6316.1 PAGE: 12 T E D -- 364 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 -- ---------------------------------------- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ........ ....................... NUMBER EXAMINED: 5 10 5 10 NO MACROSCOPIC LESIONS BLOOD FILM TAKEN DISCHARGE-OCULAR MALOCCLUSION BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN 33 5 10 01 04 5 10 03 21 5 10 00 02 5 10 01 BONE, FEMUR (FE) ............ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 A N I M A L S A F F E C T E D -- MARROW, FEMUR (FM) ................ ................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MARROW, STERNUM (SE) ............... ................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 BONE, STERNUM (SB) ................ ................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 365 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE ------------------------------ -- NUMBER: 5 10 5 10 -=- -=- -=- -=- EYE (EY) .................... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 BRAIN (BR) .................. ....................... NUMBER EXAMINED: NOT REMARKABLE: COMPRESSED (HYPOTHALAMUS) SPINAL CORD (SC) ............ ....................... NUMBER EXAMINED: NOT REMARKABLE: 5 10 59 01 5 10 5 10 5 10 58 02 5 10 5 10 LUNG (LU) ................... ....................... NUMBER EXAMINED: NOT REMARKABLE: LIGHT FOCUS(I)/AREA(S) KIDNEY (KD) ................. ....................... NUMBER EXAMINED: NOT REMARKABLE: 5 10 5 10 00 5 10 5 10 5 10 58 02 5 10 5 10 LIVER (LI) .................. ....................... NUMBER EXAMINED: NOT REMARKABLE: LIGHT FOCUS(I)/AREA(S) MOTTLED 5 10 5 10 00 00 5 10 49 01 10 Covance 6329-212 3M T-6316.1 PAGE: 2 A F F E C T E D -- 366 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- HEART (HT) .................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MUSCLE, SKELETAL (SM) ....... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 NERVE, SCIATIC (SN) ......... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, MESENTERIC (MS) ......... ....................... NUMBER EXAMINED: NOT REMARKABLE: DIFFUSELY RED SPLEEN (SP) ................. ....................... NUMBER EXAMINED: NOT REMARKABLE: 5 10 5 10 00 5 10 5 10 5 10 59 01 5 10 5 10 THYMUS (TH) ................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ESOPHAGUS (ES) .............. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 3 A F F E C T E D -- 367 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 THYROID (TY) ............................ ........... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 PARATHYROID (PT) ........................ ........... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 TRACHEA (TR) ............................ ........... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ADRENAL, CORTEX (AC) .................... ........... NUMBER EXAMINED: NOT REMARKABLE: DARK FOCUS(I)/AREA(S) ADRENAL, MEDULLA (MA) ................... ........... NUMBER EXAMINED: NOT REMARKABLE: 5 10 4 10 10 5 10 5 10 5 10 5 10 00 5 10 5 10 PITUITARY (PI) .......................... ........... NUMBER EXAMINED: NOT REMARKABLE: MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) 5 10 58 02 00 00 5 10 47 02 10 01 Covance 6329-212 3M T-6316.1 PAGE: 4 A F F E C T E D -- 368 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 HARDERIAN GLAND (HG) ........ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 SALIV GL , MANDIB (SG) ....... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 PANCREAS (PA) ............... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 STOMACH, GL (ST) ............ ....................... NUMBER EXAMINED: NOT REMARKABLE: DARK FOCUS(I)/AREA(S) STOMACH, NONGL (SU) ......... ....................... NUMBER EXAMINED: NOT REMARKABLE: 5 10 59 01 5 10 5 10 5 10 44 16 5 10 5 10 DUODENUM (DU) ............... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 JEJUNUM (JE) ................ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 5 A F F E C T E D -- 369 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- ILEUM (IL) .................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CECUM (CE) .................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 COLON (CO) .................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 RECTUM (RE) ................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 SKIN (SK) ................... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 URINARY BLADDER (UB) ........ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 PROSTATE (PR) ............... ....................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 Covance 6329-212 3M T-6316.1 PAGE: 6 A F F E C T E D -- 370 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- SEMINAL VESICLES (SV) ....... ....................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 EPIDIDYMIDES (EP) ........... ....................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 TESTIS (TE) ................. ....................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 MAMMARY, FEMALE (MF) ........ ....................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 4 8 MASS(ES) CYST(S) 0001 0012 CERVIX (CV) ................. ....................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 UTERUS (UT) ................. ....................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 3 8 LUMEN FILLED WITH FLUID CYST(S) ** CONTINUED ON NEXT PAGE ** 0011 0010 371 Covance 6329-212 3M T-6316.1 PAGE: 7 A F F E C T E D -- Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 10 5 10 -- ---------------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) ................. ....................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 3 8 THICKENED WALL 0001 OVARY (OV) .................. ....................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 9 CYST(S) 0001 VAGINA (VA) ................. ....................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 LN, MEDIASTINAL (ML) ........ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, MANDIBULAR (MN) ......... ....................... NUMBER EXAMINED: NOT REMARKABLE: DIFFUSELY RED CAVITY, ORAL (OC) ........... ....................... NUMBER EXAMINED: NOT REMARKABLE: 5 10 49 11 5 10 5 10 5 10 4 10 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 8 A F F E C T E D -- 372 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- LN, ANT MES/PANC (AP) ....... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CAVITY, ABDOM (PC) .......... ....................... NUMBER EXAMINED: NOT REMARKABLE: MASS(ES) CALVARIUM/SKULL (CA) ........ ....................... NUMBER EXAMINED: NOT REMARKABLE: 5 10 5 10 00 5 10 5 10 5 10 59 01 5 10 5 10 MAMMARY, MALE (MM) .......... ....................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 LN, LUMBAR/ILIAC (LM) ....... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ANUS (AN) ................... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, RENAL (RL) .............. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 9 A F F E C T E D -- 373 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- TAIL (TI) ................... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 59 RAISED AREA(S) 0001 PENIS (PE) .................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, INGUINAL (IN) ........... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CAVITY, THORACIC (TA) ....... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 FOOT/FOOTPAD(S) (FP) ........ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, AXILLARY (AX) ........... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, TRACHEOBRON (TB) ........ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 10 A F F E C T E D -- 374 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- ADIPOSE TISSUE (AT) ......... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 JOINT, OTHER (JT) ........... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 AORTA (AO) .................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 LN, POPLITEAL (PP) .......... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 SUBCUTANEOUS TIS (SQ) ....... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 TONGUE (TO) ................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 URETER (UE) ................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 11 A F F E C T E D -- 375 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- AORTIC ARCH (AA) ............ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 TESTIS/EPIDIDYM (TP) ........ ....................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 PINNA (PN) .................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 THYROID/PARATHYR (TT) ....... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 DIAPHRAGM (DP) .............. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 BILE DUCT (BD) .............. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 AUDITORY SEB GL (AS) ........ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 12 A F F E C T E D -- 376 Table 36 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 ADRENAL (AD) ............... ** END OF LIST ** NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 13 A F F E C T E D -- 377 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 -- ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) .............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NO MACROSCOPIC LESIONS STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN ANIMAL OBESE FLUID-ABDOMINAL CAVITY 001000002 0 0301110100 14 22 22 17 10 13 12 15 19 12 2 023101110 0100000001 13274 02242 1110100010 2 000000011 0000000100 14 22 22 17 10 13 13 15 17 12 1102 010101 36722 011 01 0000000010 BONE, FEMUR (FE) .................. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 MARROW, FEMUR (FM) ................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 MARROW, STERNUM (SE) ............... ................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 378 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M BER - O F - ANI M A LS- A F F E SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- NUMBER: 14 22 22 17 10 13 13 15 19 12 BONE, STERNUM (SB) ................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 EYE (EY) .......................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 22 17 10 13 13 15 18 12 OPAQUE CORNEA RED INTRAOCULAR MATERIAL 1100000000 0000000010 BRAIN (BR) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 12 18 19 14 6 7 7 7 11 6 COMPRESSED (HYPOTHALAMUS) 2433466886 SPINAL CORD (SC) .................. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LUNG (LU) ......................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 16 9 13 13 15 17 10 LIGHT FOCUS(I)/AREA(S) ADHESION(S) 0 0 01 1 0 0 02 2 0010000000 379 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M BER - O F - ANI M A LS- A F F E ' SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- NUMBER: 14 22 22 17 10 13 13 15 19 12 KIDNEY (KD) ......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 17 18 13 7 11 12 15 17 10 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK GRANULAR MATERIAL LARGE MASS(ES) DARK FOCUS(I)/AREA(S) SMALL 0141210001 1311010000 1301000010 130000002 0 0002 000000 0010001000 12 00000000 0000100001 0100000000 0010000000 LIVER (LI) .......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 6 7 10 4 5 8 7 10 4 4 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DIFFUSELY LIGHT MASS(ES) RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) CYST(S) ADHESION(S) 02 12 1 02 12 4 0103000010 02 4 5 1 1 01 3 1 000100004 0 0010001000 1103001030 8 11 9 3 3 3 3 5 7 5 0002 00102 0 1000000000 0000010000 380 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M BER - O F - ANI M A LS- A F F E ' SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 6 7 10 4 5 8 7 10 4 4 MASS #2 0000000011 HEART (HT) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 17 10 13 13 15 19 12 LIGHT FOCUS(I)/AREA(S) 0100000000 MUSCLE, SKELETAL (SM) ............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 17 10 13 13 15 19 12 ATROPHY-FOCAL 0100000000 NERVE, SCIATIC (SN) ............... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LN, MESENTERIC (MS) ............... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 20 15 10 11 13 12 18 11 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK RED FOCUS(I)/AREA(S) 11010102 00 0011000000 1110000110 0000010000 0000000100 381 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M BER - O F - ANI M A LS- A F F E ' SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ** FROM PREVIOUS PAGE ** LN, MESENTERIC (MS) ............... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 20 15 10 11 13 12 18 11 GELATINOUS 0000000001 SPLEEN (SP) ....................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 22 17 10 13 13 15 19 12 LARGE 1100000000 THYMUS (TH) ....................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 18 12 CYST(S) 0000000010 ESOPHAGUS (ES) .................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 THYROID (TY) ...................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 19 15 9 12 12 13 19 12 LARGE DIFFUSELY DARK 0032 1112 00 0000010000 382 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE ------------------------------------ -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PARATHYROID (PT) .................. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 22 16 10 13 13 15 19 12 LARGE 1101000000 TRACHEA (TR) ...................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 ADRENAL, CORTEX (AC) ................. .............. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 12 20 19 16 9 7 9 11 17 10 MASS(ES) DARK FOCUS(I)/AREA(S) LARGE CYST(S) DIFFUSELY DARK SMALL RED FOCUS(I)/AREA(S) RAISED AREA(S) 001 0012 011 1100000000 112 00314 1 0 0000001000 000102 0000 0000000001 0000000001 0000100000 ADRENAL, MEDULLA (MA) ................ .............. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 383 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PITUITARY (PI) ............................. ........ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 8 17 12 8 4 5 2 1 4 2 MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) LARGE RAISED AREA(S) LIGHT FOCUS(I)/AREA(S) CYST(S) 2333466786 3012112101 01 1 01 02 32 2 02 1 1 01 1 5 62 0 02 1 0 01 0 0 0 1 012 000000 0010000000 HARDERIAN GLAND (HG) ....................... ........ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 SALIV GL,, MANDIB (SG) ............ .................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 PANCREAS (PA) .................... .................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 11 21 21 17 9 13 12 15 19 12 LARGE VESSELS MASS(ES) 3000100000 0110001000 384 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- STOMACH, GL (ST) ........................... ........ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 19 21 12 9 11 13 15 16 7 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION LIGHT FOCUS(I)/AREA(S) 0 0 02 1 1 0 0 0 0 0001010011 1301000001 0012 000012 0000000011 STOMACH, NONGL (SU) ........................ ........ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 21 17 10 12 13 15 19 12 EROSION/ULCERATION THICKENED WALL 0010010000 0110010000 DUODENUM (DU) .............................. ........ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 JEJUNUM (JE) ............................... ........ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 12 15 19 12 LARGE LYMPHOID AREA(S) 0000001000 385 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ILEUM (IL) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 12 15 19 12 LARGE LYMPHOID AREA(S) 0000001000 CECUM (CE) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 17 10 13 13 15 19 12 THICKENED WALL 0010000000 COLON (CO) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 RECTUM (RE) ....................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 SKIN (SK) ......................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 12 16 17 15 8 12 12 15 18 12 MASS(ES) CRUSTED AREA(S) RAISED AREA(S) ABRASION(S)/u LCERATION(S) 02 2 1 1 01 01 0 02 3 01 1 0 0 0 0 2101000000 0110000000 386 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- URINARY BLADDER (UB) .............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 17 10 13 13 15 19 12 CALCULUS(I) 0010000000 PROSTATE (PR) ..................... ................. NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 11 19 17 16 9 0 0 0 0 0 NODULAR 3351100000 SEMINAL VESICLES (SV) ............. ................. NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 22 22 17 10 0 0 0 0 0 SMALL LARGE 1000000000 1000000000 EPIDIDYMIDES (EP) ................. ................. NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 13 22 22 17 10 0 0 0 0 0 SMALL 1000000000 TESTIS (TE) ....................... ................. NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 21 19 12 10 0 0 0 0 0 SMALL LARGE RED/YELLOW FOCUS(I)/AREA(S) 2 124 000000 0010000000 0010000000 387 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ** FROM PREVIOUS PAGE ** TESTIS (TE) ......................................... NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 21 19 12 10 0 0 0 0 0 RED FOCUS(I)/AREA(S) 0001000000 MAMMARY, FEMALE (MF) ................................ NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 3 3 4 11 8 MASS(ES) CYST(S) MASS #2 THICKENED MASS #3 MASS #4 MASS #5 0000088983 0000030003 0 0 0 0 02 4 64 2 0000054642 000002212 0 0000001100 0000000100 CERVIX (CV) ......................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 11 11 14 16 10 MASS(ES) LARGE LIGHT FOCUS(I)/AREA(S) 0000001112 0 0 0 0 02 1 01 0 0000000010 UTERUS (UT) ......................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 6 9 10 11 8 LUMEN FILLED WITH FLUID 000001002 0 388 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ** FROM PREVIOUS PAGE ** UTERUS (UT) ......................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 6 9 10 11 8 CYST(S) THICKENED WALL LARGE, DIFFUSE MASS(ES) LARGE, FOCAL RED FOCUS(I)/AREA(S) SEMISOLID MATERIAL 0 0 0 0 04 34 34 000000002 0 000002 0000 0000012 12 0 0000000010 0000000100 0000000100 OVARY (OV) .......................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 10 8 11 11 8 CYST(S) 0000035484 VAGINA (VA) ......................................... NUMBER EXAMINED: 0 0 0 0 0 13 13 15 19 12 NOT REMARKABLE: 0 0 0 0 0 12 13 14 19 11 MASS(ES) SEMIFLUID MATERIAL 0000000001 0000010100 LN, MEDIASTINAL (ML) ................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 21 17 10 13 12 15 18 12 LARGE DIFFUSELY RED 0010001010 1000001000 389 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ** FROM PREVIOUS PAGE ** LN, MEDIASTINAL (ML) .............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 21 21 17 10 13 12 15 18 12 CYST(S) 0100000000 LN, MANDIBULAR (MN) ............... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 11 16 19 16 8 11 11 11 17 7 DIFFUSELY RED LARGE MOTTLED 1500111313 11002 00000 1031011112 CAVITY, ORAL (OC) ................. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LN, ANT MES/PANC (AP) ............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 20 21 17 10 13 12 15 19 12 LARGE DIFFUSELY RED 0100000000 02 1 0 0 01 0 0 0 CAVITY, ABDOM (PC) ................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 14 19 11 MASS(ES) 0000000101 390 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- CALVARIUM/SKULL (CA) .............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 MAMMARY, MALE (MM) ................ ................. NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 21 22 14 9 0 0 0 0 0 MASS(ES) CYST(S) 2103000000 0000100000 LN, LUMBAR/ILIAC (LM) ............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 12 18 19 17 10 12 13 15 19 12 LARGE MOTTLED DIFFUSELY RED CYSTS 2330000000 0000010000 0010000000 0100000000 ANUS (AN) ......................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LN, RENAL (RL) .................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 17 10 12 12 15 18 11 LARGE DIFFUSELY RED 0000011000 0010011011 391 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 14 22 22 17 10 13 13 15 19 12 ** FROM PREVIOUS PAGE ** LN, RENAL (RL) .................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 21 17 10 12 12 15 18 11 SMALL 0000010000 TAIL (TI) ......................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 16 10 13 13 15 19 11 CRUSTED AREA(S) LARGE 0100000001 0001000000 PENIS (PE) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 LN, INGUINAL (IN) ................. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 18 12 DIFFUSELY DARK 0000000010 CAVITY, THORACIC (TA) ............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 392 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) .............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 11 17 16 15 6 10 13 15 18 10 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 04 2 2 2 3 0 012 314 02 00000 LN, AXILLARY (AX) ................. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 20 22 17 10 11 12 14 15 11 LARGE MOTTLED DIFFUSELY RED 02 0001102 0 0100010121 0000000010 LN, TRACHEOBRON (TB) .............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 ADIPOSE TISSUE (AT) ............... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 17 10 12 13 15 19 12 MASS(ES) 0100010000 JOINT, OTHER (JT) ................. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 21 17 10 12 13 14 19 12 LARGE 0110010100 393 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AORTA (AO) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 22 22 17 10 13 13 15 19 12 LARGE 1000000000 LN, POPLITEAL (PP) ................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 20 22 17 10 13 13 14 19 12 LARGE 12 000001 00 SUBCUTANEOUS TIS (SQ) ............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 TONGUE (TO) ....................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 URETER (UE) ....................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 AORTIC ARCH (AA) .................. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 394 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- TESTIS/EPIDIDYM (TP) .............. ................. NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 14 22 22 17 10 0 0 0 0 0 PINNA (PN) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 18 12 RED FOCUS(I)/AREA(S) 0000000010 THYROID/PARATHYR (TT) ............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 18 12 MISSING/NOT IDENTIFIED 0000000010 DIAPHRAGM (DP) .................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 13 22 22 17 10 13 13 15 19 12 MASS(ES) 1000000000 BILE DUCT (BD) .................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 21 22 17 10 13 13 15 19 12 LARGE 0100000000 395 Table 37 Incidence of Macroscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND KEYWORD(S) OR PHRASE ------------------------------------ -- NUMBER: 14 22 22 17 10 13 13 15 19 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AUDITORY SEB GL (AS) .............. ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 9 13 13 15 19 12 MASS(ES) 0000100000 ADRENAL (AD) ...................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 19 12 NOT REMARKABLE: 14 22 22 17 10 13 13 15 19 12 ** END OF LIST 396 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ........ ....................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NO MACROSCOPIC LESIONS DISCHARGE-ORAL STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT GI TRACT-LARGE LUMEN GI TRACT-DARK DRY CONTENT FLUID-THORACIC CAVITY TEETH MISSING/BROKEN/WORN AUTOLYSIS-SEVERE ANIMAL OBESE URINARY BLADDER-LARGE LUMEN YELLOWISH MUCOUS MEMBRANES TAIL - BENT/MISSING 0010 2213 10 4 8 10 24 16 23 25 13 6 7 10 4 2 5 10 2247 6288 10 6 7 9 0000 1111 0000 1011 0103 0011 5505 3 0 02 0001 0001 00 21 17 1 18 16 25 23 07 08 10 02 01 01 01 00 04 02 00 00 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 BONE, FEMUR (FE) ............ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 Covance 6329-212 3M T-6316.1 PAGE: 1 397 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) .......... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 MARROW, STERNUM (SE) ........ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 BONE, STERNUM (SB) .......... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 EYE (EY) .................... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 33 25 27 32 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL COLLAPSED GLOBE-ANTE MORTEM 2211 1000 0110 BRAIN (BR) .................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 11 12 9 9 COMPRESSED (HYPOTHALAMUS) 25 16 19 24 SPINAL CORD (SC) ............ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 00 00 00 3 30 3 12 0 18 3 30 3 30 1 1 0 0 0 1 1 0 1 1 Covance 6329-212 3M T-6316.1 PAGE: 2 A F F E C T E D -- 398 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 36 28 28 33 3 30 1 LUNG (LU) ............................... ........... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 30 23 27 30 DIFFUSELY RED MOTTLED UNCOLLAPSED LIGHT FOCUS(I)/AREA(S) ADHESION(S) RED FOCUS(I)/AREA(S) MASS(ES) 02 01 0011 1210 2 001 1 1 02 1000 1 0 02 KIDNEY (KD) ............................. ........... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 25 21 24 23 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MOTTLED SEMIFLUID MATERIAL-PELVIS EROSION(S)/ULCERATION(S) DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK CALCULUS(I) GRANULAR MATERIAL LARGE ADHESION(S) MASS(ES) ** CONTINUED ON NEXT PAGE ** 8337 4 005 1111 0011 0000 3 0 02 2000 0000 1210 1 0 02 1300 1000 0101 3 30 1 23 21 01 02 02 00 02 00 3 30 3 23 03 02 00 00 01 01 00 01 01 00 01 00 00 1 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 3 399 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 -- ---------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) ............................. ........... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 25 21 24 23 3 23 1 DEPRESSED FOCUS(I)/AREA(S) 1000000 LIVER (LI) .............................. ........... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 27 20 18 20 3 30 1 23 1 1 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DIFFUSELY LIGHT MASS(ES) RED FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE DARK FOCUS(I)/AREA(S) CYST(S) 02 1 3 03 0 1311210 4 1512 00 0004 010 1101010 1110000 3234 02 0 0000010 0010000 0001000 HEART (HT) .............................. ........... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 32 3 30 3 30 1 1 LARGE MOTTLED 0001000 0001000 Covance 6329-212 3M T-6316.1 PAGE: 4 400 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- MUSCLE, SKELETAL (SM) ....... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 28 27 33 3 30 3 29 1 1 MASS(ES) DIFFUSELY RED 1000010 0010000 NERVE, SCIATIC (SN) ......... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 LN, MESENTERIC (MS) ......... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 33 28 28 32 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK SMALL 1000 1000 0001 1000 1000 SPLEEN (SP) ................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 27 28 30 SMALL LARGE ROUGH SURFACE CYST(S) 0001 1001 0100 0001 3 30 3 30 00 00 00 00 00 3 30 3 30 00 00 00 00 1 1 0 0 0 0 0 1 1 0 0 0 0 401 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- THYMUS (TH) ................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 34 27 28 30 3 30 2 29 1 1 RED FOCUS(I)/AREA(S) MASS(ES) DIFFUSELY RED MISSING/NOT IDENTIFIED 0100110 1001000 0001000 1001000 ESOPHAGUS (ES) .............. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 THYROID (TY) ................ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 27 33 LARGE RED FOCUS(I)/AREA(S) 0000 0010 PARATHYROID (PT) ............ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 32 LARGE 0001 TRACHEA (TR) ................ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 29 01 00 3 30 3 29 01 3 30 3 30 1 1 0 0 1 1 0 1 1 Covance 6329-212 3M T-6316.1 PAGE: 6 402 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- ADRENAL, CORTEX (AC) ........ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 33 27 25 31 3 30 3 30 1 1 MASS(ES) LARGE SMALL MISSING/NOT IDENTIFIED MOTTLED 2000000 0110000 0 02 1 0 0 0 0001000 1000000 ADRENAL, MEDULLA (MA) ....... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 PITUITARY (PI) ...................... ............... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 8 10 7 9 MASS(ES) RED FOCUS(I)/AREA(S) LARGE DIFFUSELY RED 28 16 19 24 0110 0100 0010 HARDERIAN GLAND (HG) ................ ............... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 38 0 18 02 02 00 3 30 3 30 1 1 0 0 0 0 1 1 SALIV GL, MANDIB (SG) ............... ............... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 Covance 6329-212 3M T-6316.1 PAGE: 7 A F F E C T E D -- 403 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- PANCREAS (PA) ........................... ........... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 31 3 30 3 29 1 1 MASS(ES) 0002 010 STOMACH, GL (ST) ........................ ........... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 25 26 24 24 3 30 2 24 1 0 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION MASS(ES) 7225151 2 001 000 3003000 2 011 01 0 0010000 STOMACH, NONGL (SU) ..................... ........... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 30 24 26 26 3 30 3 25 1 1 LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL DARK FOCUS(I)/AREA(S) 0001000 322504 0 32 02 030 1100010 DUODENUM (DU) ........................... ........... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 Covance 6329-212 3M T-6316.1 PAGE: 8 404 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- JEJUNUM (JE) ................ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 ILEUM (IL) .................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 CECUM (CE) .................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 28 28 33 RED FOCUS(I)/AREA(S) 1000 COLON (CO) .................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 00 3 30 3 30 1 1 0 1 1 RECTUM (RE) ................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 SKIN (SK) ................... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 25 27 32 MASS(ES) CRUSTED AREA(S) RAISED AREA(S) 02 01 0110 1000 3 30 3 27 02 01 00 1 1 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 9 A F F E C T E D -- 405 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 36 28 28 33 3 30 1 URINARY BLADDER (UB) ........ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 34 27 28 29 THICKENED WALL CALCULUS(I) SEMIFLUID MATERIAL RED FOCUS(I)/AREA(S) DIFFUSELY RED 1 1 02 2 002 0002 0001 0001 PROSTATE (PR) ............... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 30 26 22 24 LARGE NODULAR DIFFUSELY RED SMALL MOTTLED GELATINOUS MASS(ES) 0002 6264 0001 0002 0000 0001 0001 SEMINAL VESICLES (SV) ....... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 27 25 24 25 SMALL GELATINOUS LARGE MOTTLED ** CONTINUED ON NEXT PAGE ** 5234 1101 3014 1010 3 30 3 28 00 01 00 00 02 3 30 3 24 00 04 00 01 01 00 00 3 30 3 17 09 02 03 01 1 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 1 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 10 T E D -- 406 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 ---- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SEMINAL VESICLES (SV) ....... ....................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 27 25 24 25 3 17 1 DIFFUSELY RED 0001000 EPIDIDYMIDES (EP) ........... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 28 28 33 3 30 3 29 1 1 SMALL 1000010 TESTIS (TE) ................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 32 26 25 29 3 30 3 24 1 1 SMALL CONTAINS FLUID DIFFUSELY RED LARGE RED/YELLOW FOCUS(I)/AREA(S) 4223060 0010000 0001000 1000000 1000000 MAMMARY, FEMALE (MF) ........ ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 CERVIX (CV) .................... .................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 11 A F F E C T E D -- 407 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- UTERUS (UT) ................. ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 OVARY (OV) .................. ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 VAGINA (VA) ................. ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, MEDIASTINAL (ML) ........ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 28 27 32 LARGE DIFFUSELY RED 1001 1011 LN, MANDIBULAR (MN) ......... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 33 27 28 31 DIFFUSELY RED LARGE 2 002 1100 3 30 3 30 00 00 3 30 3 28 01 01 1 1 0 0 1 1 0 0 Covance 6329-212 3M T-6316.1 PAGE: 12 A F F E C T E D -- 408 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- CAVITY, ORAL (OC) ........... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 27 28 33 3 30 3 30 1 1 FRACTURE(S) 0100000 LN, ANT MES/PANC (AP) ....... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 27 28 32 3 30 3 30 1 1 LARGE MOTTLED DIFFUSELY RED 1100000 1000000 0001000 CAVITY, ABDOM (PC) .......... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 27 33 3 30 3 29 1 1 MASS(ES) 0010010 CALVARIUM/SKULL (CA) ........ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 MAMMARY, MALE (MM) .......... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 28 24 26 30 MASS(ES) CYST(S) THICKENED 22 02 5210 1011 3 30 3 28 01 01 00 1 1 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 13 T E D -- 409 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- LN, LUMBAR/ILIAC (LM) ....... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 33 23 27 29 3 30 3 30 1 1 LARGE MOTTLED DIFFUSELY RED 34 14 000 2100000 0100000 ANUS (AN) ................... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 29 1 1 MASS(ES) 0000010 LN, RENAL (RL) .............. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 32 28 28 32 3 30 3 29 1 1 LARGE MOTTLED CYST(S) 4 00001 0 1000000 0001000 TAIL (TI) ................... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 34 25 27 32 3 30 3 27 1 1 RAISED AREA(S) CRUSTED AREA(S) ABRASION/ULCERATIONS DIFFUSELY DARK 1100010 1111010 0100000 0000010 T E D -- 410 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- PENIS (PE) .................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 27 28 33 3 30 3 30 1 1 PROTRUDING 1100000 LN, INGUINAL (IN) ........... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 CAVITY, THORACIC (TA) ....... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 FOOT/FOOTPAD(S) (FP) ........ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 27 26 22 28 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 5232 4 033 LN, AXILLARY (AX) ........... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 24 05 02 3 30 3 30 1 1 0 0 1 1 LN, TRACHEOBRON (TB) ........ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 31 DIFFUSELY RED ** CONTINUED ON NEXT PAGE ** 0002 3 30 3 30 00 1 1 0 Covance 6329-212 3M T-6316.1 PAGE: 15 T E D -- 411 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 36 28 28 33 3 30 1 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, TRACHEOBRON (TB) ........ ....................... NUMBER EXAMINED: 36 28 28 33 3 30 1 NOT REMARKABLE: 36 28 28 31 3 30 1 LARGE 0001000 ADIPOSE TISSUE (AT) ......... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 32 3 30 3 30 1 1 THICKENED 0001000 JOINT, OTHER (JT) ........... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 28 28 32 3 30 3 30 1 1 LARGE 1001000 AORTA (AO) .................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 34 28 28 33 3 30 3 30 1 1 LARGE THICKENED WALL 2000000 1000000 LN, POPLITEAL (PP) .......... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 27 33 3 30 3 30 1 1 LARGE 0010000 T E D -- 412 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ....... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 TONGUE (TO) ................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 URETER (UE) ................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 LARGE 0000 AORTIC ARCH (AA) ............ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 35 28 28 33 LARGE 1000 TESTIS/EPIDIDYM (TP) ........ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 28 02 3 30 3 30 00 3 30 3 30 1 1 0 1 1 0 1 1 PINNA (PN) .................. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 CRUSTED AREA(S) 0000 3 30 3 29 01 1 1 0 Covance 6329-212 3M T-6316.1 PAGE: 17 A F F E C T E D -- 413 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 36 28 28 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- THYROID/PARATHYR (TT) ....... ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 DIAPHRAGM (DP) .............. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 BILE DUCT (BD) .............. ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 AUDITORY SEB GL (AS) ........ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 3 30 3 30 1 1 ADRENAL (AD) ................ ....................... NUMBER EXAMINED: 36 28 28 33 NOT REMARKABLE: 36 28 28 33 ** END OF LIST ** 3 30 3 30 1 1 Covance 6329-212 3M T-6316.1 PAGE: 18 A F F E C T E D -- 414 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ........ ....................... NUMBER EXAMINED: 37 37 35 30 0 28 0 DISCHARGE-ORAL STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT GI TRACT-LARGE LUMEN GI TRACT-DARK DRY CONTENT FLUID-THORACIC CAVITY TEETH MISSING/BROKEN/WORN AUTOLYSIS-SEVERE ANIMAL OBESE TAIL - BENT/MISSING 0112 7 7 14 12 31 31 29 24 18 19 19 12 3 5 5 11 2856 342 9 11 10 18 12 0100 0000 0010 0010 1101 0010 1110 0000 01 05 0 22 0 11 03 05 05 09 00 01 01 02 01 00 01 01 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 BONE, FEMUR (FE) ............ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 MARROW, FEMUR (FM) ................ ................. NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 Covance 6329-212 3M T-6316.1 PAGE: 19 T E D -- 415 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 37 37 35 30 0 28 0 -=- -=- -=- -=- -=- -=- -=- MARROW, STERNUM (SE) ........ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 BONE, STERNUM (SB) .......... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 EYE (EY) .................... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 34 30 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL 0010 0000 BRAIN (BR) .................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 5 5 6 8 COMPRESSED (HYPOTHALAMUS) 32 32 29 22 SPINAL CORD (SC) ............ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 26 01 01 0 28 0 10 0 18 0 28 0 28 0 0 0 0 0 0 0 0 0 LUNG (LU) ................... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 36 35 34 26 DIFFUSELY RED ** CONTINUED ON NEXT PAGE ** 1110 0 28 0 24 00 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 20 A F F E C T E D -- 416 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 -- ---------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................... ........... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 36 35 34 26 0 24 0 UNCOLLAPSED LIGHT FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) MASS(ES) 0111000 000302 0 0000010 0000010 KIDNEY (KD) ............................. ........... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 30 35 31 25 0 28 0 25 0 0 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MOTTLED DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK CALCULUS(I) GRANULAR MATERIAL LIGHT FOCUS(I)/AREA(S) 4130010 0000010 1101000 0000010 1002 000 0002 000 1000000 3010010 0000010 LIVER (LI) .............................. ........... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 28 27 32 17 0 28 0 19 0 0 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED ** CONTINUED ON NEXT PAGE ** 011302 0 1102 000 12 05 03 0 Covance 6329-212 3M T-6316.1 PAGE: 21 T E D -- 417 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 37 37 35 30 0 28 0 -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .............................. ........... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 28 27 32 17 0 19 0 DIFFUSELY DARK DEPRESSED FOCUS(I)/AREA(S) DIFFUSELY LIGHT RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) CYST(S) 0003010 0100000 410102 0 451102 0 0001010 0010000 0001000 HEART (HT) .............................. ........... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 29 0 28 0 28 0 0 DIFFUSELY LIGHT 0001000 MUSCLE, SKELETAL (SM) ................... ........... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 T E D -- NERVE, SCIATIC (SN) ................ ................ NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 418 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 37 37 35 30 0 28 0 -=- -=- -=- -=- -=- -=- -=- LN, MESENTERIC (MS) ......... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 35 36 32 29 0 28 0 28 0 0 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK 0100000 1011000 1010000 0010000 SPLEEN (SP) ................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 36 37 34 27 0 28 0 26 0 0 SMALL LARGE LIGHT FOCUS(I)/AREA(S) 1000010 0012 01 0 0001000 THYMUS (TH) ................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 36 36 35 30 0 28 0 28 0 0 MOTTLED MISSING/NOT IDENTIFIED LARGE 1000000 0100000 1000000 ESOPHAGUS (ES) .............. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 Covance 6329-212 3M T-6316.1 PAGE: 23 T E D -- 419 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 37 37 35 30 0 28 0 THYROID (TY) DIFFUSELY RED MASS(ES) LARGE PARATHYROID (PT) LARGE TRACHEA (TR) NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 36 36 35 29 0100 1000 0001 NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 29 0001 NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 00 00 00 0 28 0 28 00 0 28 0 28 0 0 0 0 0 0 0 0 0 0 ADRENAL, CORTEX (AC) DIFFUSELY RED DARK FOCUS(I)/AREA(S) LARGE CYST(S) LIGHT FOCUS(I)/AREA(S) DIFFUSELY DARK SMALL RED FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 30 27 27 27 1210 0010 5741 0010 0112 1001 0110 2100 0 28 0 24 00 01 03 00 00 00 00 00 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 24 T E D -- 420 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 25 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ........ ....................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 30 27 27 27 0 24 0 DIFFUSELY LIGHT 0010000 ADRENAL, MEDULLA (MA) ....... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 T E D -- PITUITARY (PI) ....................... .............. NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 4 4 1 6 MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) LARGE 32 30 29 22 1032 0110 1430 HARDERIAN GLAND (HG) ................. .............. NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 05 0 18 01 02 03 0 28 0 28 0 0 0 0 0 0 0 0 SALIV GL, MANDIB (SG) ............... ............... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 421 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 26 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ------------------------------------------ -- NUMBER: 37 37 35 30 0 28 0 -=- -=- -=- -=- -=- -=- -=- PANCREAS (PA) ........................... ........... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 36 35 30 0 28 0 28 0 0 LARGE VESSELS 0100000 STOMACH, GL (ST) ........................ ........... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 34 32 32 24 0 28 0 27 0 0 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION LIGHT FOCUS(I)/AREA(S) 1225010 02 00000 02 00000 1011000 1000000 STOMACH, NONGL (SU) ..................... ........... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 31 32 34 26 0 28 0 23 0 0 LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL DARK FOCUS(I)/AREA(S) RAISED AREA(S) 3000000 131304 0 02 0102 0 1101010 1000000 DUODENUM (DU) ........................... ........... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 T E D -- 422 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- JEJUNUM (JE) ................ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 36 35 35 30 0 28 0 27 0 0 MASS(ES) DIVERTICULUM(A) 0000010 12 00000 ILEUM (IL) .................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 CECUM (CE) .................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 36 37 35 30 SMALL 1000 COLON (CO) .................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 00 0 28 0 28 0 0 0 0 0 RECTUM (RE) ................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 SKIN (SK) ................... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 35 34 29 ALOPECIA-FOCAL ** CONTINUED ON NEXT PAGE ** 0001 0 28 0 26 00 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 27 A F F E C T E D -- 423 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 28 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ................... ....................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 37 35 34 29 0 26 0 MASS(ES) CRUSTED AREA(S) ALOPECIA-DIFFUSE 0110010 0100010 0000010 URINARY BLADDER (UB) ........ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 T E D -- PROSTATE (PR) ............... ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) ....... ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) ........... ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TESTIS (TE) ................. ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 424 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 37 37 35 30 0 28 0 MAMMARY, FEMALE (MF) ................................ NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 13 12 14 16 MASS(ES) CYST(S) MASS #2 THICKENED MASS #3 MASS #4 MASS #5 MASS #6 20 19 17 11 8851 4695 10 11 9 4 1233 0133 0011 0001 CERVIX (CV) ......................................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 35 35 33 29 MASS(ES) LARGE FIRM 0110 2111 0010 UTERUS (UT) ......................................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 29 31 31 22 LUMEN FILLED WITH FLUID CYST(S) THICKENED WALL LARGE, DIFFUSE MASS(ES) ** CONTINUED ON NEXT PAGE ** 1015 5622 1011 1 0 02 2000 0 28 0 11 0 14 01 02 04 00 00 00 00 0 28 0 27 01 00 00 0 28 0 25 01 01 00 01 01 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 29 T E D -- 425 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 30 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 37 37 35 30 0 28 0 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) ................. ....................... NUMBER EXAMINED: 37 37 35 30 0 28 0 NOT REMARKABLE: 29 31 31 22 0 25 0 SEMIFLUID MATERIAL 0001000 OVARY (OV) .................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 34 31 28 18 0 28 0 22 0 0 CYST(S) MISSING/NOT IDENTIFIED MASS(ES) MASS #2 3 5 7 10 0 6 0 0101000 0001000 0001000 VAGINA (VA) ................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 35 32 31 27 0 28 0 22 0 0 MASS(ES) SEMIFLUID MATERIAL 002 0010 2523050 LN, MEDIASTINAL (ML) ........ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 27 0 0 DIFFUSELY RED 0000010 T E D -- 426 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 37 37 35 30 0 28 0 -=- -=- -=- -=- -=- -=- -=- LN, MANDIBULAR (MN) ......... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 33 34 31 25 0 28 0 26 0 0 DIFFUSELY RED LARGE MOTTLED MISSING/NOT IDENTIFIED 3112 01 0 122 002 0 0012 000 0001000 CAVITY, ORAL (OC) ........... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 LN, ANT MES/PANC (AP) ....... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 CAVITY, ABDOM (PC) .......... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 34 35 29 MASS(ES) 0301 CALVARIUM/SKULL (CA) ........ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 36 35 30 DEPRESSED AREA(S) 0100 0 28 0 27 01 0 28 0 28 00 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 31 T E D -- 427 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 37 37 35 30 0 28 0 -=- -=- -=- -=- -=- -=- -=- MAMMARY, MALE (MM) .......... ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, LUMBAR/ILIAC (LM) ....... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 36 36 34 30 LARGE MOTTLED 1010 1100 ANUS (AN) ................... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 25 03 00 0 28 0 28 0 0 0 0 0 0 LN, RENAL (RL) .............. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 LARGE 0000 TAIL (TI) ................... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 34 28 CRUSTED AREA(S) 0012 PENIS (PE) .................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 27 01 0 28 0 28 00 0 28 0 28 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 32 T E D -- 428 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 37 37 35 30 0 28 0 LN, INGUINAL (IN) ........... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 36 36 35 29 LARGE DIFFUSELY RED DIFFUSELY DARK 1101 1100 0001 CAVITY, THORACIC (TA) ....... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 34 30 MASS(ES) 0010 FOOT/FOOTPAD(S) (FP) ........ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 33 34 32 30 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 3030 1300 LN, AXILLARY (AX) ........... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 28 31 31 28 LARGE MOTTLED CYST(S) DIFFUSELY RED 4 031 4511 1000 12 01 0 28 0 27 01 00 00 0 28 0 28 00 0 28 0 26 02 00 0 28 0 27 01 00 00 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 33 T E D -- 429 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 37 37 35 30 0 28 0 -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) ........ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 ADIPOSE TISSUE (AT) ......... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 JOINT, OTHER (JT) ........... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 35 37 35 30 LARGE 2000 AORTA (AO) .................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 FIRM 0000 LN, POPLITEAL (PP) .......... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 00 0 28 0 27 01 0 28 0 28 0 0 0 0 0 0 0 0 SUBCUTANEOUS TIS (SQ) ....... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 34 30 MASS(ES) 0010 0 28 0 28 00 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 34 A F F E C T E D -- 430 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 37 37 35 30 0 28 0 -=- -=- -=- -=- -=- -=- -=- TONGUE (TO) ................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 27 0 0 MASS(ES) 0000010 URETER (UE) ................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 AORTIC ARCH (AA) ............ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 TESTIS/EPIDIDYM (TP) ........ ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PINNA (PN) .................. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 THYROID/PARATHYR (TT) ....... ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 DIAPHRAGM (DP) .............. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 Covance 6329-212 3M T-6316.1 PAGE: 35 A F F E C T E D -- 431 Table 38 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;SUBSET=ALL N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ------------------------------ -- NUMBER: 37 37 35 30 0 28 0 -=- -=- -=- -=- -=- -=- -=- BILE DUCT (BD) .............. ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 AUDITORY SEB GL (AS) ........ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 0 28 0 28 0 0 ADRENAL (AD) ................ ....................... NUMBER EXAMINED: 37 37 35 30 NOT REMARKABLE: 37 37 35 30 ** END OF LIST ** 0 28 0 28 0 0 Covance 6329-212 3M T-6316.1 PAGE: 36 A F F E C T E D -- 432 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ........ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NO MACROSCOPIC LESIONS DISCHARGE-ORAL STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT BONE MARROW SMEAR TAKEN GI TRACT-LARGE LUMEN GI TRACT-DARK DRY CONTENT FLUID-THORACIC CAVITY TEETH MISSING/BROKEN/WORN AUTOLYSIS-SEVERE ANIMAL OBESE URINARY BLADDER-LARGE LUMEN YELLOWISH MUCOUS MEMBRANES TAIL - BENT/MISSING 14 7 12 12 2213 10 7 8 11 43 38 45 52 15 6 9 14 4 3 5 10 3 5 6 18 7398 12 6 7 9 0000 29 27 27 32 1111 0000 1011 12 08 0011 8 11 7 7 3 0 02 0001 0001 50 21 18 1 28 17 25 27 08 08 10 5 10 02 01 01 01 00 06 02 00 00 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 BONE, FEMUR (FE) ............ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 Covance 6329-212 3M T-6316.1 PAGE: 1 433 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) .......... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 MARROW, STERNUM (SE) ........ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 BONE, STERNUM (SB) .......... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 EYE (EY) .................... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 66 56 59 69 13 40 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL COLLAPSED GLOBE-ANTE MORTEM 331100 100000 011000 BRAIN (BR) .................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 43 40 38 42 13 18 COMPRESSED (HYPOTHALAMUS) 27 20 22 28 0 22 SPINAL CORD (SC) ............ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 0 0 0 1 1 0 1 1 Covance 6329-212 3M T-6316.1 PAGE: 2 A F F E C T E D -- 434 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 13 40 1 LUNG (LU) ............................... ........... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 63 55 58 66 2 32 DIFFUSELY RED MOTTLED UNCOLLAPSED LIGHT FOCUS(I)/AREA(S) ADHESION(S) RED FOCUS(I)/AREA(S) MASS(ES) 02 0121 101101 12 1 0 02 2 002 03 1112 00 1 0 0 0 02 1002 00 KIDNEY (KD) ............................. ........... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 57 48 52 56 13 30 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MOTTLED SEMIFLUID MATERIAL-PELVIS EROSION(S)/ULCERATION(S) DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK CALCULUS(I) GRANULAR MATERIAL LARGE ADHESION(S) MASS(ES) ** CONTINUED ON NEXT PAGE ** 94 7 8 05 5 3 1 6 02 111100 001100 000001 430301 330000 0002 01 121001 1 012 00 250001 100000 010101 1 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 3 435 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 -- ---------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) ............................. ........... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 57 48 52 56 13 30 1 DEPRESSED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) SMALL 1000000 0100000 0010000 LIVER (LI) .............................. ........... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 52 37 38 44 7 28 1 1 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DEPRESSED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) RED FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE DARK FOCUS(I)/AREA(S) CYST(S) 042514 0 1414710 5396210 0005210 0000100 1111010 2213000 11 13 12 7 0 5 0 0000010 0012 000 1001000 HEART (HT) .............................. ........... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 59 60 69 13 40 1 1 LARGE MOTTLED ** CONTINUED ON NEXT PAGE ** 0001000 0001000 Covance 6329-212 3M T-6316.1 PAGE: 4 436 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE ** FROM PREVIOUS PAGE ** HEART (HT) ................. LIGHT FOCUS(I)/AREA(S) MUSCLE, SKELETAL (SM) .. MASS(ES) DIFFUSELY RED ATROPHY-FOCAL NERVE, SCIATIC (SN) -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 13 40 1 NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 59 60 69 13 40 010000 NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 69 59 59 70 13 39 100001 001000 010000 NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 0 1 1 0 0 0 1 1 LN, MESENTERIC (MS) LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK SMALL NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 66 59 58 67 13 40 210100 101100 111100 100000 100000 1 1 0 0 0 0 0 437 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 13 40 1 SPLEEN (SP) ................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 68 58 60 67 13 40 SMALL LARGE ROUGH SURFACE CYST(S) 000100 210100 010000 000100 THYMUS (TH) ................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 67 59 60 67 1 39 RED FOCUS(I)/AREA(S) MOTTLED MASS(ES) DIFFUSELY RED MISSING/NOT IDENTIFIED 010011 100000 100100 000100 100100 ESOPHAGUS (ES) .............. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 0 0 0 0 1 1 0 0 0 0 0 1 1 THYROID (TY) ........................ ............... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 56 68 13 38 LARGE RED FOCUS(I)/AREA(S) 0032 02 001000 1 1 0 0 438 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ------------------------------ -- NUMBER: 70 60 60 70 13 40 1 -=- -=- -=- -=- -=- -=- -=- PARATHYROID (PT) ............ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 69 59 60 68 13 39 1 1 LARGE 1102 010 TRACHEA (TR) ................ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 ADRENAL, CORTEX (AC) ................................ NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 64 57 54 67 1 39 DIFFUSELY RED MASS(ES) DARK FOCUS(I)/AREA(S) LARGE DIFFUSELY DARK SMALL MISSING/NOT IDENTIFIED MOTTLED RAISED AREA(S) 000010 2 01 000 210000 123000 000100 0 02 1 0 0 000100 100000 000001 ADRENAL, MEDULLA (MA) ............................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 0 0 0 0 0 0 0 0 0 1 1 Covance 6329-212 3M T-6316.1 PAGE: 7 A F F E C T E D -- 439 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 70 60 60 70 13 40 1 -=- -=- -=- -=- -=- -=- -=- PITUITARY (PI) .......................... ........... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 36 37 29 35 13 12 1 1 MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) LARGE DIFFUSELY RED RAISED AREA(S) LIGHT FOCUS(I)/AREA(S) CYST(S) 30 19 22 29 3012 022 0 0311 0010 0 02 1 1012 0010 0 22 01 03 02 00 00 00 00 0 0 0 0 0 0 0 0 HARDERIAN GLAND (HG) .................... ........... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 SALIV GL, MANDIB (SG) ............... ............... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 PANCREAS (PA) .................... .................. NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 67 59 59 68 13 38 LARGE VESSELS MASS(ES) 300001 0112 01 1 1 0 0 Covance 6329-212 3M T-6316.1 PAGE: 8 440 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 70 60 60 70 13 40 1 -=- -=- -=- -=- -=- -=- -=- STOMACH, GL (ST) ........................ ........... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 57 54 55 55 3 33 1 0 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION MASS(ES) 7228261 3 1 02 1 0 0 4304 000 2 023010 0010000 STOMACH, NONGL (SU) ..................... ........... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 64 55 57 63 13 35 1 1 LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL DARK FOCUS(I)/AREA(S) 0001000 323504 0 3312 03 0 1100010 DUODENUM (DU) ........................... ........... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 JEJUNUM (JE) ................... .................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 ILEUM (IL) .................... ..................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 Covance 6329-212 3M T-6316.1 PAGE: 9 441 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 13 40 1 -=- -=- -=- -=- -=- -=- -=- CECUM (CE) .................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 69 60 59 70 12 40 1 1 DIFFUSELY RED RED FOCUS(I)/AREA(S) THICKENED WALL 0000100 1000000 0010000 COLON (CO) .................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 RECTUM (RE) .................... .................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 SKIN (SK) .............................. ............ NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 67 51 54 66 13 35 RED FOCUS(I)/AREA(S) MASS(ES) CRUSTED AREA(S) RAISED AREA(S) a b r a s i o n (s )/u l c e r a t i o n (s ) 000100 0422 03 034 002 310100 011000 1 1 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 10 T E D -- 442 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 13 40 1 URINARY BLADDER (UB) ........ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 68 59 59 66 13 38 THICKENED WALL CALCULUS(I) SEMIFLUID MATERIAL RED FOCUS(I)/AREA(S) DIFFUSELY RED 1102 00 2 012 01 0002 00 000100 0 0 01 02 PROSTATE (PR) ............... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 60 55 49 60 13 33 LARGE NODULAR DIFFUSELY RED SMALL MOTTLED GELATINOUS MASS(ES) 1002 00 9 5 11 5 0 5 000100 0002 01 000001 000100 000100 SEMINAL VESICLES (SV) ....... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 58 57 56 62 13 27 SMALL GELATINOUS LARGE MOTTLED ** CONTINUED ON NEXT PAGE ** 62 34 09 1 1 01 02 5 014 03 101001 1 1 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 1 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 11 T E D -- 443 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SEMINAL VESICLES (SV) ....... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 58 57 56 62 13 27 1 DIFFUSELY RED 0001000 EPIDIDYMIDES (EP) ........... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 68 60 60 70 13 39 1 1 SMALL 2 00001 0 TESTIS (TE) ................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 64 57 54 61 13 34 1 1 SMALL CONTAINS FLUID DIFFUSELY RED LARGE RED/YELLOW FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) 6347060 0010000 0001000 1010000 1010000 0001000 MAMMARY, FEMALE (MF) ........ ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 CERVIX (CV) .................... .................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 12 A F F E C T E D -- 444 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 13 40 1 -=- -=- -=- -=- -=- -=- -=- UTERUS (UT) ................. ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 OVARY (OV) .................. ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 VAGINA (VA) ................. ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, MEDIASTINAL (ML) ........ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 68 59 58 69 13 40 LARGE DIFFUSELY RED CYST(S) 101100 2 011 00 010000 LN, MANDIBULAR (MN) ......... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 61 51 57 66 13 36 DIFFUSELY RED LARGE MOTTLED 6 7 03 02 22 0003 103100 1 1 0 0 0 1 1 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 13 T E D -- 445 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 13 40 1 -=- -=- -=- -=- -=- -=- -=- CAVITY, ORAL (OC) ........... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 59 60 70 13 40 1 1 FRACTURE(S) 0100000 LN, ANT MES/PANC (AP) ....... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 69 57 59 69 13 40 1 1 LARGE MOTTLED DIFFUSELY RED 12 00000 1000000 02 1 1 0 0 0 CAVITY, ABDOM (PC) .......... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 59 70 1 39 1 1 EDEMA MASS(ES) 0000100 0010010 CALVARIUM/SKULL (CA) ........ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 MAMMARY, MALE (MM) .......... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 60 55 58 64 13 37 MASS(ES) CYST(S) ** CONTINUED ON NEXT PAGE ** 430501 52 1 0 02 1 1 0 0 Covance 6329-212 3M T-6316.1 PAGE: 14 T E D -- 446 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 ---- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, MALE (MM) .......... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 60 55 58 64 13 37 1 THICKENED 1011000 LN, LUMBAR/ILIAC (LM) ....... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 65 51 56 66 13 40 1 1 LARGE MOTTLED DIFFUSELY RED CYSTS 5744 000 2100000 0110000 0100000 ANUS (AN) ................... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 39 1 1 MASS(ES) 0000010 LN, RENAL (RL) .............. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 66 60 59 69 13 39 1 1 LARGE MOTTLED CYST(S) DIFFUSELY RED 4 00001 0 1000000 0001000 0010000 T E D -- 447 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 13 40 1 -=- -=- -=- -=- -=- -=- -=- TAIL (TI) ................... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 68 56 59 68 13 37 1 1 RAISED AREA(S) CRUSTED AREA(S) ABRASION/ULCERATIONS DIFFUSELY DARK LARGE 1100010 1211010 0100000 0000010 0001000 PENIS (PE) .................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 69 59 60 70 13 40 1 1 PROTRUDING 1100000 LN, INGUINAL (IN) ........... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 CAVITY, THORACIC (TA) ....... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 FOOT/FOOTPAD(S) (FP) ........ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 58 53 48 63 13 30 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 5654 07 7 1 7 3 04 1 1 0 0 Covance 6329-212 3M T-6316.1 PAGE: 16 T E D -- 448 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 13 40 1 -=- -=- -=- -=- -=- -=- -=- LN, AXILLARY (AX) ........... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 58 60 70 13 40 1 1 LARGE MOTTLED 02 00000 0100000 LN, TRACHEOBRON (TB) ........ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 68 13 40 1 1 DIFFUSELY RED LARGE 0002 000 0001000 ADIPOSE TISSUE (AT) ......... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 59 60 69 13 40 1 1 THICKENED MASS(ES) 0001000 0100000 JOINT, OTHER (JT) ........... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 69 59 59 69 13 40 1 1 LARGE 1111000 AORTA (AO) .................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 67 60 60 70 13 40 1 1 LARGE ** CONTINUED ON NEXT PAGE ** 3000000 Covance 6329-212 3M T-6316.1 PAGE: 17 T E D -- 449 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 13 40 1 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** AORTA (AO) .................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 1 NOT REMARKABLE: 67 60 60 70 13 40 1 THICKENED WALL 1000000 LN, POPLITEAL (PP) .......... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 69 58 59 70 13 40 1 1 LARGE 1210000 SUBCUTANEOUS TIS (SQ) ....... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 TONGUE (TO) ................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 URETER (UE) ................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 38 LARGE 000002 AORTIC ARCH (AA) ............ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 69 60 60 70 13 40 LARGE 100000 1 1 0 1 1 0 Covance 6329-212 3M T-6316.1 PAGE: 18 A F F E C T E D -- 450 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 13 40 1 -=- -=- -=- -=- -=- -=- -=- TESTIS/EPIDIDYM (TP) ........ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 PINNA (PN) .................. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 39 CRUSTED AREA(S) 000001 THYROID/PARATHYR (TT) ....... ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 0 1 1 DIAPHRAGM (DP) .............. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 69 60 60 70 13 40 MASS(ES) 100000 BILE DUCT (BD) .............. ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 59 60 70 13 40 LARGE 010000 AUDITORY SEB GL (AS) ........ ....................... NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 39 MASS(ES) 000001 1 1 0 1 1 0 1 1 0 Covance 6329-212 3M T-6316.1 PAGE: 19 A F F E C T E D -- 451 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: --- --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 13 40 1 ADRENAL (AD) ............... ** END OF LIST ** NUMBER EXAMINED: 70 60 60 70 13 40 NOT REMARKABLE: 70 60 60 70 13 40 1 1 Covance 6329-212 3M T-6316.1 PAGE: 20 A F F E C T E D -- 452 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 -- --------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ......... ...................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NO MACROSCOPIC LESIONS DISCHARGE-ORAL STAINS-PERINEUM/PERIANAL BLOOD FILM TAKEN DISCHARGE-OCULAR DISCHARGE-NASAL MALOCCLUSION STAINS - SKIN/HAIRCOAT ANIMAL THIN GI TRACT-DARK FLUID CONTENT BONE MARROW SMEAR TAKEN URINARY BLADDER-SMALL LUMEN TRACHEA-CONTENT FOAMY MATTER GI TRACT-LARGE LUMEN GI TRACT-DARK DRY CONTENT FLUID-THORACIC CAVITY TEETH MISSING/BROKEN/WORN AUTOLYSIS-SEVERE ANIMAL OBESE TAIL - BENT/MISSING FLUID-ABDOMINAL CAVITY 14 9 7 12 0113 8 7 15 12 49 43 44 53 18 20 20 13 3 5 5 12 2 10 7 12 3 4 2 11 11 10 18 13 0110 28 18 20 32 0010 0001 0000 0010 0010 2112 0010 122 0 0000 0001 60 01 05 0 34 0 11 04 07 05 0 10 00 5 12 00 00 01 01 02 02 00 02 01 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 BONE, FEMUR (FE) ............. ...................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 Covance 6329-212 3M T-6316.1 PAGE: 21 T E D -- 453 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 10 40 0 -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) .......... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 MARROW, STERNUM (SE) ........ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 BONE, STERNUM (SB) .......... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 EYE (EY) .................... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 59 69 10 38 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL RED INTRAOCULAR MATERIAL 001001 000001 000100 BRAIN (BR) .................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 32 22 23 37 10 16 LACERATION COMPRESSED (HYPOTHALAMUS) 0001 38 38 37 32 00 0 24 SPINAL CORD (SC) ............ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 22 T E D -- 454 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 10 40 0 T E D -- LUNG (LU) ............................... ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 69 58 59 61 10 34 DIFFUSELY RED MOTTLED UNCOLLAPSED LIGHT FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) MASS(ES) 111000 000100 0112 00 0 0 07 04 000001 000001 KIDNEY (KD) ............................. ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 61 57 56 63 10 35 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MOTTLED DIFFUSELY LIGHT CYST(S) DIFFUSELY DARK CALCULUS(I) GRANULAR MATERIAL MASS(ES) LIGHT FOCUS(I)/AREA(S) 5 1 3 0 02 100001 110100 000101 1004 00 0002 00 100000 311001 000001 000001 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 455 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 10 40 0 LIVER (LI) .............................. ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 55 44 52 41 7 23 LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DEPRESSED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) CYST(S) ADHESION(S) MASS #2 032606 11 032 0 321814 000721 010000 42 0102 010300 786807 010301 001000 000100 100000 000101 HEART (HT) .............................. ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 69 10 40 DIFFUSELY LIGHT 000100 MUSCLE, SKELETAL (SM) ................... ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 24 T E D -- 456 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE -- NUMBER: 70 60 60 70 70 40 0 -=- -=- -=- -=- -=- -=- -=- NERVE, SCIATIC (SN) ................................. NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 LN, MESENTERIC (MS) .................. .............. NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 66 59 54 67 10 39 LARGE MOTTLED DIFFUSELY RED DIFFUSELY DARK RED FOCUS(I)/AREA(S) GELATINOUS 112 000 101100 1022 00 101000 001000 000001 SPLEEN (SP) .......................... .............. NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 69 60 59 67 10 38 SMALL LARGE LIGHT FOCUS(I)/AREA(S) 100001 0012 01 000100 THYMUS (TH) .......................... .............. NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 69 59 60 69 9 40 RED FOCUS(I)/AREA(S) MOTTLED MISSING/NOT IDENTIFIED ** CONTINUED ON NEXT PAGE ** 000010 100000 010000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 25 A F F E C T E D -- 457 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 26 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYMUS (TH) ................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 69 59 60 69 9 40 0 LARGE CYST(S) 1000000 0001000 ESOPHAGUS (ES) .............. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 T E D -- THYROID (TY) ..................... . ................. NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 68 58 58 69 10 40 DIFFUSELY RED MASS(ES) LARGE DIFFUSELY DARK 010000 100000 112100 100000 PARATHYROID (PT) ................. . ................. NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 69 10 40 LARGE 000100 TRACHEA (TR) ..................... . ................. NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 0 0 0 0 0 0 0 0 0 458 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 27 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 10 40 0 T E D -- ADRENAL, CORTEX (AC) .................... ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 57 46 48 65 10 34 DIFFUSELY RED MASS(ES) DARK FOCUS(I)/AREA(S) LARGE CYST(S) LIGHT FOCUS(I)/AREA(S) DIFFUSELY DARK SMALL RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT 121000 12 01 01 001001 8882 03 011000 0112 00 300100 011001 210001 001000 ADRENAL, MEDULLA (MA) ................... ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PITUITARY (PI) ...................................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 28 16 12 27 10 7 MISSING/NOT IDENTIFIED MASS(ES) DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) LARGE RAISED AREA(S) 0001 38 36 36 32 3242 0343 2586 0100 00 0 24 02 04 05 00 0 0 0 0 0 0 0 0 459 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE -- -- NUMBER: 70 60 60 70 10 40 0 -=- -=- -=- -=- -=- -=- -=- HARDERIAN GLAND (HG) .................... ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 SALIV GL, MANDIB (SG) ................... ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 PANCREAS (PA) ........................... ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 58 60 70 10 40 LARGE VESSELS MASS(ES) 010000 010000 STOMACH, GL (ST) ........................ ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 63 55 55 54 9 34 DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) THICKENED WALL EROSION/ULCERATION LIGHT FOCUS(I)/AREA(S) 3 2 3 11 1 1 22 12 01 02 0001 1012 02 100101 STOMACH, NONGL (SU) ..................... ........... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 63 55 59 66 10 35 LIGHT FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 300000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 28 T E D -- 460 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 29 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) ......... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 63 55 59 66 10 35 0 EROSION/ULCERATION THICKENED WALL DARK FOCUS(I)/AREA(S) RAISED AREA(S) 231304 0 12 0102 0 1101010 1000000 DUODENUM (DU) ............... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 T E D -- JEJUNUM (JE) ......................... .............. NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 69 57 60 70 10 39 MASS(ES) DIVERTICULUM(A) LARGE LYMPHOID AREA(S) 000001 12 0000 010000 ILEUM (IL) ........................... .............. NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 59 60 70 10 40 LARGE LYMPHOID AREA(S) 010000 0 0 0 0 0 0 0 0 461 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 -- ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- CECUM (CE) .................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 69 60 60 70 10 40 0 0 SMALL 1000000 COLON (CO) .................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 RECTUM (RE) ................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 SKIN (SK) ................... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 69 57 58 68 10 38 ALOPECIA-FOCAL MASS(ES) CRUSTED AREA(S) ALOPECIA-DIFFUSE 001100 02 1 1 01 110001 000001 URINARY BLADDER (UB) ........ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 0 0 0 0 0 0 PROSTATE (PR) ............... ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 30 T E D -- 462 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE -- NUMBER: 70 60 60 70 10 40 0 -=- -=- -=- -=- -=- SEMINAL VESICLES (SV) ............................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) ................. ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TESTIS (TE) .................... .................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 MAMMARY, FEMALE (MF) ............... ................ NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 35 25 28 46 10 19 MASS(ES) CYST(S) MASS #2 THICKENED MASS #3 MASS #4 MASS #5 MASS #6 28 27 26 20 12 8 5 3 6 10 15 9 15 15 15 8 3445 02 4 3 0 02 1 0001 0 17 04 04 06 00 00 00 00 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 31 A F F E C T E D -- 463 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 32 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 10 40 0 -=- -=- -=- -=- -=- -=- -=- CERVIX (CV) ................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 66 56 57 66 10 37 0 0 MASS(ES) LARGE FIRM LIGHT FOCUS(I)/AREA(S) 02 2 1 03 0 42 12 000 0010000 0001000 UTERUS (UT) ................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 53 50 50 52 9 33 0 0 LUMEN FILLED WITH FLUID CYST(S) THICKENED WALL LARGE, DIFFUSE MASS(ES) SEMIFLUID MATERIAL LARGE, FOCAL RED FOCUS(I)/AREA(S) SEMISOLID MATERIAL 3 02 8 1 1 0 10 9 6 5 0 5 0 1 014 000 3002 010 32 12 01 0 0001000 0001000 0010000 0010000 OVARY (OV) .................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 63 49 49 49 10 30 0 0 CYST(S) MISSING/NOT IDENTIFIED MASS(ES) ** CONTINUED ON NEXT PAGE ** 7 10 11 19 0101 0001 0 10 00 00 0 0 0 T E D -- 464 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 33 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 60 60 70 10 40 0 ---- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** OVARY (OV) .................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 0 NOT REMARKABLE: 63 49 49 49 10 30 0 MASS #2 0001000 VAGINA (VA) ................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 67 55 55 67 10 33 0 0 MASS(ES) SEMIFLUID MATERIAL 002 002 0 3533050 LN, MEDIASTINAL (ML) ........ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 59 60 69 10 39 0 0 LARGE DIFFUSELY RED 0101000 0100010 LN, MANDIBULAR (MN) ......... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 62 54 51 63 10 33 0 0 DIFFUSELY RED RED FOCUS(I)/AREA(S) LARGE MOTTLED MISSING/NOT IDENTIFIED 625304 0 0100000 122 002 0 112302 0 0001000 T E D -- 465 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 10 40 0 -=- -=- -=- -=- -=- -=- -=- CAVITY, ORAL (OC) ........... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 LN, ANT MES/PANC (AP) ....... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 59 60 70 10 40 DIFFUSELY RED 010000 CAVITY, ABDOM (PC) .......... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 57 59 68 10 38 MASS(ES) 0312 02 CALVARIUM/SKULL (CA) ........ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 59 60 69 10 40 FRACTURE(S) DEPRESSED AREA(S) 000100 010000 MAMMARY, MALE (MM) .......... ....................... NUMBER EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 34 T E D -- 466 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 10 40 0 -=- -=- -=- -=- -=- -=- -=- LN, LUMBAR/ILIAC (LM) ....... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 68 59 59 70 10 37 0 0 LARGE MOTTLED 1010030 2100000 ANUS (AN) ................... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 LN, RENAL (RL) .............. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 69 59 60 69 10 38 LARGE DIFFUSELY RED SMALL 110001 110101 100000 TAIL (TI) ................... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 59 67 10 39 RAISED AREA(S) CRUSTED AREA(S) 000100 0012 01 PENIS (PE) .................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 35 T E D -- 467 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 10 40 0 LN, INGUINAL (IN) ........... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 69 59 60 68 10 39 LARGE DIFFUSELY RED DIFFUSELY DARK 110101 110000 0002 00 CAVITY, THORACIC (TA) ....... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 59 70 10 40 MASS(ES) 001000 FOOT/FOOTPAD(S) (FP) ........ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 63 57 57 69 10 36 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 6 03 1 04 130000 LN, AXILLARY (AX) ........... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 59 53 55 64 10 38 LARGE MOTTLED CYST(S) DIFFUSELY RED 513301 552301 100000 12 02 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 36 T E D -- 468 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 10 40 0 -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) ........ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 ADIPOSE TISSUE (AT) ......... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 69 60 60 70 10 40 MASS(ES) 100000 JOINT, OTHER (JT) ........... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 67 60 59 70 10 40 LARGE 301000 AORTA (AO) .................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 39 FIRM 000001 LN, POPLITEAL (PP) .......... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 59 70 10 40 LARGE 001000 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 37 T E D -- 469 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 60 60 70 10 40 0 -=- -=- -=- -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ....... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 59 70 10 40 0 0 MASS(ES) 0010000 TONGUE (TO) ................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 39 0 0 MASS(ES) 0000010 URETER (UE) ................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 AORTIC ARCH (AA) ............ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 TESTIS/EPIDIDYM (TP) ........ ....................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PINNA (PN) .................. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 69 10 40 RED FOCUS(I)/AREA(S) 000100 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 38 A F F E C T E D -- 470 Table 39 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 10 40 0 THYROID/PARATHYR (TT) ....... ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 69 10 40 MISSING/NOT IDENTIFIED 000100 DIAPHRAGM (DP) .............. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 0 0 0 BILE DUCT (BD) .............. ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 AUDITORY SEB GL (AS) ........ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 0 0 ADRENAL (AD) ................ ....................... NUMBER EXAMINED: 70 60 60 70 10 40 NOT REMARKABLE: 70 60 60 70 10 40 ** END OF LIST ** 0 0 Covance 6329-212 3M T-6316.1 PAGE: 39 A F F E C T E D -- 471 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-212 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e II o f X II Page 472 of 4873 Covance 6329-212 3M T-6316.1 VOLUME II OF XII CONTENTS Page TABLES 4 0 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - W e e k 8 S a c r i f i c e ...................................................4 7 4 4 1 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - W e e k 1 4 S a c r i f i c e ............................................... 4 8 6 4 2 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - W e e k 5 3 S a c r i f i c e ............................................... 4 9 9 4 3 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - T e r m i n a l a n d R e c o v e r y S a c r i f i c e s ..... 5 1 4 4 4 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - U n s c h e d u l e d D e a t h s ...........................................5 3 7 4 5 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - A l l A n i m a l s ...............................................................5 8 3 4 6 In c id e n ce o f S e v e rity o f S e lected M ic ro sc o p ic O b se rv a tio n s - W e e k 8 S a c r i f i c e ...................................................................................................................................................................................6 3 5 4 7 In c id e n ce o f S e v e rity o f S e lected M ic ro sc o p ic O b se rv a tio n s - W e e k 14 S a c r i f i c e ...................................................................................................................................................................................6 4 1 48 In c id e n ce o f S e v e rity o f S e lected M ic ro sc o p ic O b se rv a tio n s - W e e k 53 S a c r i f i c e ...................................................................................................................................................................................6 4 7 4 9 In c id e n ce o f S e v e rity o f S e lected M ic ro sc o p ic O b se rv a tio n s - T erm in al an d R e c o v e r y S a c r i f i c e ........................................................................................................................................................6 5 3 50 In c id e n ce o f S e v e rity o f S e lected M ic ro sc o p ic O b se rv a tio n s - U n sc h e d u le d D e a t h s ...................................................................................................................................................................................... 6 6 1 5 1 I n c i d e n c e o f S e v e r i t y o f S e l e c t e d M i c r o s c o p i c O b s e r v a t i o n s - A l l A n i m a l s ............6 6 8 A P P E N D I X 1 ..........................................................................................................................................................................................6 7 7 P r o t o c o l D e v i a t i o n s ....................................................................................................................................................................6 7 8 473 TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 -- -------------------------------------- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ...................... ..................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 BRAIN (BR) .................... ..................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LIVER (LI) .................... ..................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 3 0 4 0 --HEMORRHAGE --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --VACUOLATION, HEPATOCELLULAR 0301 0515 2000 0301 0100 LIVER, OIL RED O (LI1) ........ ..................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 KIDNEY (KD) ................................. ....... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 3 4 5 4 --CAST, PROTEINACEOUS --CYST --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE 1000 1100 0001 0100 Covance 6329-212 3M T-6316.1 PAGE: 1 A F F E C T E D -- 474 TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE ( N-ETHYL PERFLUOROOCTANESULFONAMIDO !ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION NUMB E R -< SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5NUMBER: 5 5 5 5 SPLEEN (SP) ................. ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 PANCREAS (PA) ............... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ADRENAL, CORTEX (AC) ........ ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ADRENAL, MEDULLA (MA) ....... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 LN, MESENTERIC (MS) ......... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 5 5 5 --HEMORRHAGE 1000 OVARY (OV) .................. ....................... NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 5 TESTIS (TE) ................. ....................... NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 475 Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- BONE, FEMUR (FE) ............ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MARROW, FEMUR (FM) .......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MARROW, STERNUM (SE) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 BONE, STERNUM (SB) .......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SPINAL CORD (SC) ............ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 THYMUS (TH) ................. ....................... NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 LUNG (LU) ................... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 476 Covance 6329-212 3M T-6316.1 PAGE: 3 A F F E C T E D -- TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- HEART (HT) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MUSCLE, SKELETAL (SM) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 NERVE, SCIATIC (SN) ......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TRACHEA (TR) ................ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ESOPHAGUS (ES) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 THYROID (TY) ................ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PARATHYROID (PT) ............ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 477 Covance 6329-212 3M T-6316.1 PAGE: 4 A F F E C T E D -- TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- PITUITARY (PI) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 HARDERIAN GLAND (HG) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SALIV GL, MANDIB (SG) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 STOMACH, GL (ST) ............ ....................... NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 STOMACH, NONGL (SU) ......... ....................... NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 DUODENUM (DU) ............... ....................... NUMBER EXAMINED: 4 1 0 0 NOT REMARKABLE: 4 1 0 0 JEJUNUM (JE) ................ ....................... NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 478 Covance 6329-212 3M T-6316.1 PAGE: 5 A F F E C T E D -- TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- ILEUM (IL) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CECUM (CE) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 COLON (CO) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 RECTUM (RE) ................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SKIN (SK) ................... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MAMMARY, FEMALE (MF) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 URINARY BLADDER (UB) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 479 Covance 6329-212 3M T-6316.1 PAGE: 6 A F F E C T E D -- TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 -- ---------------------------------------- -=- -=- -=- -=- CERVIX (CV) ................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 UTERUS (UT) ................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 VAGINA (VA) ................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PROSTATE (PR) ............... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SEMINAL VESICLES (SV) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 EPIDIDYMIDES (EP) ........... ....................... NUMBER EXAMINED: 1 0 0 0 NOT REMARKABLE: 1 0 0 0 CAUSE OF DEATH (CD) ......... ....................... NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 0 0 0 0 --SCHEDULED SACRIFICE 5555 480 Covance 6329-212 3M T-6316.1 PAGE: 7 A F F E C T E D -- TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- LN, MANDIBULAR (MN) ......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, MEDIASTINAL (ML) ........ ....................... NUMBER EXAMINED: 0 0 0 1 NOT REMARKABLE: 0 0 0 0 --HEMORRHAGE 0001 CAVITY, ABDOM (PC) .......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, ORAL (OC) ........... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 HEMATO NEOPLASIA (HN) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MESENTERY (MY) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 481 Covance 6329-212 3M T-6316.1 PAGE: 8 A F F E C T E D -- TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- LN, LUMBAR/ILIAC (LM) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, RENAL (RL) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MAMMARY, MALE (MM) .......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, AXILLARY (AX) ........... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CALVARIUM/SKULL (CA) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TAIL (TI) ................... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ANUS (AN) ................... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 482 Covance 6329-212 3M T-6316.1 PAGE: 9 A F F E C T E D -- TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, INGUINAL (IN) ........... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 JOINT, OTHER (JT) ........... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ADIPOSE TISSUE (AT) ......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTA (AO) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PENIS (PE) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PREPUTIAL GLAND (PG) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 483 Covance 6329-212 3M T-6316.1 PAGE: 10 A F F E C T E D -- TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- AUDITORY SEB GL (AS) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTIC ARCH (AA) ............ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, TRACHEOBRON (TB) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 URETER (UE) ................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PINNA (PN) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, POPLITEAL (PP) .......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, THORACIC (TA) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 484 Covance 6329-212 3M T-6316.1 PAGE: 11 A F F E C T E D -- TABLE 40 Incidence of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 5 5 5 -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TONGUE (TO) ................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, OTHER (LN) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 DIAPHRAGM (DP) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 BILE DUCT (BD) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ** END OF LIST ** Covance 6329-212 3M T-6316.1 PAGE: 12 A F F E C T E D -- 485 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: ---MALE--- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 ** TOP OF LIST ** -=- -=- -=- -=- -=- -=- -=- -=- EYE (EY) ............................................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 BRAIN (BR) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 LIVER (LI) .......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 1 1 0 3 1 3 2 --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, CHRONIC --VACUOLATION, HEPATOCELLULAR 10000000 10001000 01 3 5 0 0 02 11301422 02 2 1 0 0 0 0 LIVER, OIL RED O (LI1) .............................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 KIDNEY (KD) ......................................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 3 5 5 5 5 4 4 --CAST, PROTEINACEOUS --CYST --DEGENERATION, TUBULAR --DILATATION, PELVIC 10000000 00000001 01000000 10000000 486 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -- MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 -- ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** KIDNEY (KD) ....................... ................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 3 5 5 5 5 4 4 --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --MINERALIZATION, TUBULAR --PYELONEPHRITIS 10000000 01000000 00000010 10000000 SPLEEN (SP) ....................... ................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 PANCREAS (PA) ....................... ............... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 4 5 5 5 5 5 --FIBROSIS --PIGMENT 10000000 10100000 ADRENAL, CORTEX (AC) ................ ............... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 2 4 4 5 5 5 4 --VACUOLATION 23110001 ADRENAL, MEDULLA (MA) ............... ............... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 487 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: --- MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- LN, MESENTERIC (MS) .................. .............. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 4 5 5 5 4 5 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 00100010 00000010 OVARY (OV) ........................... .............. NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 5 4 --HEMORRHAGE 00000001 TESTIS (TE) .......................... .............. NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 4 5 0 0 0 0 --ATROPHY/DEGENERATION 00100000 BONE, FEMUR (FE) ..................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MARROW, FEMUR (FM) ................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MARROW, STERNUM (SE) ................. .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 3 488 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S A F F E C T E D -- SEX: --- MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- BONE, STERNUM (SB) .............. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SPINAL CORD (SC) ................ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 THYMUS (TH) ..................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LUNG (LU) ....................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 HEART (HT) ...................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MUSCLE, SKELETAL (SM) ........... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 NERVE, SCIATIC (SN) ............. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 489 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S A F F E C T E D -- SEX: --- MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- TRACHEA (TR) .................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ESOPHAGUS (ES) .................. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 THYROID (TY) .................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PARATHYROID (PT) ................ ................... NUMBER EXAMINED: 1 0 0 0 0 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 0 PITUITARY (PI) .................. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 HARDERIAN GLAND (HG) ............ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SALIV GL, MANDIB (SG) ........... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 490 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S A F F E C T E D -- SEX: ---MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- STOMACH, GL (ST) ................ ................... NUMBER EXAMINED: 1 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 --EDEMA 10000000 STOMACH, NONGL (SU) ............. ................... NUMBER EXAMINED: 1 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 --EDEMA --EROSION --HEMORRHAGE 10000000 10000000 10000000 DUODENUM (DU) ................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 JEJUNUM (JE) .................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ILEUM (IL) ...................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CECUM (CE) ...................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 491 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S A F F E C T E D -- SEX: --- MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- COLON (CO) ...................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 RECTUM (RE) ..................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SKIN (SK) ....................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MAMMARY, FEMALE (MF) ............ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 URINARY BLADDER (UB) ............ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CERVIX (CV) ..................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 UTERUS (UT) ..................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 492 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- NUMB E R -O F -A N IM A L S SEX: --- MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- VAGINA (VA) ..................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PROSTATE (PR) ................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) ........... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) ............... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CAUSE OF DEATH (CD) ............. ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 0 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE 55555555 LN, MANDIBULAR (MN) ............. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, MEDIASTINAL (ML) ............ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 8 493 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S A F F E C T E D -- SEX: --- MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- CAVITY, ABDOM (PC) .............. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CAVITY, ORAL (OC) ............... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 HEMATO NEOPLASIA (HN) ........... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 MESENTERY (MY) .................. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ........... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, LUMBAR/ILIAC (LM) ........... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, RENAL (RL) .................. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 494 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S A F F E C T E D -- SEX: --- MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- MAMMARY, MALE (MM) .............. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, AXILLARY (AX) ............... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CALVARIUM/SKULL (CA) ............ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 TAIL (TI) ....................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ANUS (AN) ....................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 FOOT/FOOTPAD(S) (FP) ............ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, INGUINAL (IN) ............... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 495 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S A F F E C T E D -- SEX: --- MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- JOINT, OTHER (JT) ............... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ADIPOSE TISSUE (AT) ............. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 AORTA (AO) ...................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PENIS (PE) ...................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) ............ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) ............ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 AORTIC ARCH (AA) ................ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 496 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S A F F E C T E D -- SEX: --- MALE --- --- --- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 5 5 5 5 5 5 5 -=- -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) ............ ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 URETER (UE) ..................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 PINNA (PN) ...................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 LN, POPLITEAL (PP) .............. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 CAVITY, THORACIC (TA) ........... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 SUBCUTANEOUS TIS (SQ) ........... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 TONGUE (TO) ..................... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 497 TABLE 41 Incidence of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL ;GROUP=1,2,3,4;WEEKS=ALL DEATH=3 ;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S A F F E C T E D -- SEX: -- MALE --- --- --- --FEMALE GROUP: -1- -2- -3- -4- -1- -2- -3 - -4- NUMBER: 5 5 5 5 5 5 5 5 LN, OTHER (LN) .................. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 DIAPHRAGM (DP) .................. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 BILE DUCT (BD) .................. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 ** END OF LIST ** 498 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL ;GROUP=1,4;WEEKS=ALL DEATH=4 ;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE -- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ** TOP OF LIST ** EYE (EY) ............................................ NUMBER EXAMINED: NOT REMARKABLE: 5 10 5 10 5 10 5 10 BRAIN (BR) ....................................... ... NUMBER EXAMINED: NOT REMARKABLE: --COMPRESSION, VENTRAL LIVER (LI) ....................................... ... NUMBER EXAMINED: NOT REMARKABLE: --DEGENERATION, CYSTIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --STASIS, BILE --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR LIVER, OIL RED O (LI1) ........................... ... NUMBER EXAMINED: NOT REMARKABLE: 5 10 5 10 00 5 10 20 00 00 00 0 10 31 01 01 09 00 00 00 5 10 58 02 5 10 42 01 01 01 07 11 02 02 02 01 00 00 499 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 KIDNEY (KD) --CAST, PROTEINACEOUS --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE SPLEEN (SP) ........................ NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 5 10 07 31 00 11 00 21 5 10 5 10 5 10 15 03 20 22 21 00 5 10 5 10 PANCREAS (PA) --ATROPHY, ACINAR --FIBROSIS --INFLAMMATION, CHRONIC --PIGMENT ADRENAL, CORTEX (AC) .... --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --FIBROSIS --HEMORRHAGE NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 5 10 48 10 01 01 01 5 10 39 00 10 00 10 5 10 5 10 00 00 00 00 5 10 08 20 02 01 40 500 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 -- ------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................. .............. NUMBER EXAMINED: 5 10 5 10 NOT REMARKABLE: 3 9 0 8 --HYPERTROPHY, CORTICAL CELL --ONE EXAMINED --VACUOLATION 1000 0010 1100 ADRENAL, MEDULLA (MA) ................ .............. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 5 10 4 10 --HEMORRHAGE --ONE EXAMINED --B-PHEOCHROMOCYTOMA 0100 0010 0100 LN, MESENTERIC (MS) .................. .............. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 5 10 59 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 0101 0100 OVARY (OV) ........................... .............. NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 7 --CYST --HEMORRHAGE 0002 0002 Covance 6329-212 3M T-6316.1 PAGE: 3 A F F E C T E D -- 501 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- TESTIS (TE) ................. ....................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 9 0 0 --ATROPHY/DEGENERATION 0100 BONE, FEMUR (FE) ............ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MARROW, FEMUR (FM) .......... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MARROW, STERNUM (SE) ........ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 BONE, STERNUM (SB) .......... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 SPINAL CORD (SC) ............ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 4 A F F E C T E D -- 502 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION -- NUMBER: 5 10 5 10 -- -=- -=- -=- -=- THYMUS (TH) ................................. ....... NUMBER EXAMINED: 5 9 5 10 NOT REMARKABLE: 4 9 5 9 --FIBROSIS --HEMORRHAGE 0001 1000 LUNG (LU) ................................... ....... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 4 7 5 10 48 --HYPERPLASIA, MEDIAL, ARTERIAL/ARTERIOLAR --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION, VASCULAR 0100 1112 0001 1100 HEART (HT) .................................. ....... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 5 10 5 10 --DEGENERATION/NECROSIS 0100 MUSCLE, SKELETAL (SM) ....................... ....... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 NERVE, SCIATIC (SN) ......................... ....... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 503 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION -- NUMBER: 5 10 5 10 -------------------------------------- -=- -=- -=- -=- TRACHEA (TR) .................. ..................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ESOPHAGUS (ES) ................ ..................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 THYROID (TY) .................. ..................... NUMBER EXAMINED: NOT REMARKABLE: --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL PARATHYROID (PT) .............. ..................... NUMBER EXAMINED: NOT REMARKABLE: --HYPERPLASIA PITUITARY (PI) ................ ..................... NUMBER EXAMINED: NOT REMARKABLE: --CYST --HEMORRHAGE --HYPERPLASIA --B-ADENOMA 5 10 37 01 22 00 00 5 10 4 10 10 5 10 34 00 02 03 23 5 10 33 00 26 01 01 59 59 00 5 10 35 01 01 00 24 504 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- HARDERIAN GLAND (HG) ........ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 59 --INFLAMMATION, CHRONIC 0001 SALIV GL, MANDIB (SG) ....... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 STOMACH, GL (ST) ............ ....................... NUMBER EXAMINED: NOT REMARKABLE: --EDEMA --EROSION STOMACH, NONGL (SU) ......... ....................... NUMBER EXAMINED: NOT REMARKABLE: 5 10 59 00 01 5 10 5 10 5 10 48 10 02 5 10 5 10 DUODENUM (DU) ............... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 JEJUNUM (JE) ................ ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 7 A F F E C T E D -- 505 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- ILEUM (IL) .................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CECUM (CE) .................. ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 COLON (CO) .................. ....................... NUMBER EXAMINED: NOT REMARKABLE: --PARASITISM RECTUM (RE) ................. ....................... NUMBER EXAMINED: NOT REMARKABLE: --PARASITISM SKIN (SK) ................... ....................... NUMBER EXAMINED: NOT REMARKABLE: 5 10 59 01 5 10 59 01 5 10 5 10 5 10 5 10 00 5 10 4 10 10 5 10 5 10 MAMMARY, FEMALE (MF) ........ ....................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 3 8 --GALACTOCELE --B-FIBROADENOMA --M-CARCINOMA 0 02 1 0010 0001 Covance 6329-212 3M T-6316.1 PAGE: 8 A F F E C T E D -- 506 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION -- NUMBER: 5 10 5 10 ----------------------------------- -=- -=- -=- -=- URINARY BLADDER (UB) ............. .................. NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 CERVIX (CV) ...................... .................. NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 UTERUS (UT) ...................... .................. NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 3 8 --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL 0 02 1 0001 VAGINA (VA) ...................... .................. NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 PROSTATE (PR) .................... .................. NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 3 10 0 0 --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE 1000 1000 SEMINAL VESICLES (SV) ............ .................. NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 Covance 6329-212 3M T-6316.1 PAGE: 9 A F F E C T E D -- 507 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 5 10 5 10 -=- -=- -=- -=- EPIDIDYMIDES (EP) .................... .............. NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 T E D -- CAUSE OF DEATH (CD) .................. .............. NUMBER EXAMINED: NOT REMARKABLE: --SCHEDULED SACRIFICE LN, MANDIBULAR (MN) .................. .............. NUMBER EXAMINED: NOT REMARKABLE: --HEMORRHAGE LN, MEDIASTINAL (ML) ................. .............. NUMBER EXAMINED: NOT REMARKABLE: --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED CAVITY, ABDOM (PC) ................... .............. NUMBER EXAMINED: NOT REMARKABLE: --FIBROSIS --NECROSIS, FAT 5 10 00 5 10 11 00 11 00 00 00 00 00 00 00 00 5 10 00 5 10 10 00 10 02 00 02 02 01 00 01 01 508 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- CAVITY, ORAL (OC) ........... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 HEMATO NEOPLASIA (HN) ....... ....................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MESENTERY (MY) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, LUMBAR/ILIAC (LM) ....... ....................... NUMBER EXAMINED: 0 0 0 1 NOT REMARKABLE: 0 0 0 1 LN, RENAL (RL) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 MAMMARY, MALE (MM) .......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 11 A F F E C T E D -- 509 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- LN, AXILLARY (AX) ............ ...................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CALVARIUM/SKULL (CA) ......... ...................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TAIL (TI) .................... ...................... NUMBER EXAMINED: 0 0 0 1 NOT REMARKABLE: 0 0 0 0 --CYST, EPIDERMAL INCLUSION --INFLAMMATION, ACUTE 0001 0001 ANUS (AN) .................... ...................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 FOOT/FOOTPAD(S) (FP) ......... ...................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, INGUINAL (IN) ............ ...................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 12 A F F E C T E D -- 510 TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- JOINT, OTHER (JT) ........... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ADIPOSE TISSUE (AT) ......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTA (AO) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PENIS (PE) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PREPUTIAL GLAND (PG) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AUDITORY SEB GL (AS) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 AORTIC ARCH (AA) ............ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 511 Covance 6329-212 3M T-6316.1 PAGE: 13 A F F E C T E D -- TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION ---- NUMBER: 5 10 5 10 -=- -=- -=- -=- LN, TRACHEOBRON (TB) ........ ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 URETER (UE) ................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 PINNA (PN) .................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 LN, POPLITEAL (PP) .......... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 CAVITY, THORACIC (TA) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 SUBCUTANEOUS TIS (SQ) ....... ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 TONGUE (TO) ................. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 512 Covance 6329-212 3M T-6316.1 PAGE: 14 A F F E C T E D -- TABLE 42 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL ;GROUP=1,4;WEEKS=ALL DEATH=4 ;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S SEX: --MALE -- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 LN, OTHER (LN) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 DIAPHRAGM (DP) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 BILE DUCT (BD) .............. ....................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 ** END OF LIST ** Covance 6329-212 3M T-6316.1 PAGE: 15 A F F E C T E D -- 513 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 -- --------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ........................... ................ NUMBER EXAMINED: 14 1 0 17 0 13 0 0 20 0 NOT REMARKABLE: 12 0 0 16 0 11 0 0 17 0 --CATARACT --HEMORRHAGE --HYPERPLASIA, CORNEAL EPITHELIUM --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CILIARY --INFLAMMATION, ACUTE, CORNEAL --MINERALIZATION, CORNEAL --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 1000000000 0000000010 1000000010 0000010000 0100000010 1101000000 1000000000 010002 0000 0100000010 BRAIN (BR) ......................... ................ NUMBER EXAMINED: 14 4 3 17 4 13 6 8 20 6 NOT REMARKABLE: 12 0 1 13 0 8 1 1 11 1 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --B-GRANULAR CELL TUMOR 2423455795 0101033561 0001000000 LIVER (LI) ......................... ................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 4 3 1 1 2 6 2 7 3 5 --CYST --CYST, BILIARY --DEGENERATION, CYSTIC --FIBROSIS --FIBROSIS, CAPSULAR 1000000010 0000001000 3 5 12 12 1 0 2 0 1 1 0010100001 0000010000 514 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M BER - O F - ANI M A LS- A F F E ' SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ** FROM PREVIOUS PAGE ** LIVER (LI) .......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 4 3 1 1 2 6 2 7 3 5 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMORRHAGE --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --PIGMENT --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --M-CARCINOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1110000100 3 10 11 5 1 0 4 2 5 1 0 02 1 0 02 1 1 0 02 1 12 1 1 1 01 4764111371 0005000050 0000100000 4 13 12 8 3 1 2 2 8 1 02 01001002 012 011 01 01 0111100010 262 0012 044 0100000010 4 8 9 13 1 4 6 2 13 2 1213001142 0000000010 1000000000 LIVER, OIL RED O (LI1) .............................. NUMBER EXAMINED: 5 0 0 5 0 5 0 0 5 0 NOT REMARKABLE: 1 0 0 1 0 0 0 0 0 0 --POSITIVE OIL RED O STAINING 4 004 050050 515 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 14 22 22 17 10 13 13 15 20 12 ---------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) ......................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 0 0 1 6 0 0 1 3 4 1 --BACTERIAL COLONIES --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --INFARCT --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --THROMBUS --M-LIPOSARCOMA 0000000001 2752358364 143000002 0 0141210001 02 1 01 0 0 0 0 0 1100000000 0001000000 1000000000 1 2 4 1 0 12 10 10 13 8 0000000001 12 14 15 8 7 2 0 1 4 3 0100010011 1000000000 0000100000 SPLEEN (SP) ......................................... NUMBER EXAMINED: 14 1 0 17 0 13 0 0 20 0 NOT REMARKABLE: 13 1 0 17 0 12 0 0 20 0 --PIGMENT, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010000 1000000000 PANCREAS (PA) ....................................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 5 8 14 8 3 10 8 12 18 9 --ATROPHY, ACINAR 6767513223 516 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 -- ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PANCREAS (PA) ..................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 5 8 14 8 3 10 8 12 18 9 --CYST --HEMORRHAGE --HYPERPLASIA, ACINAR --HYPERTROPHY, VASCULAR --INFILTRATE, EOSINOPHILS --INFLAMMATION, CHRONIC --INFLAMMATION, VASCULAR --PIGMENT --B-ADENOMA, ISLET CELL --M-CARCINOMA, ISLET CELL 0100000000 2100011000 12 0001 0000 2 0001 00000 1000000000 2000000000 22 001 00000 02222 00000 1523114110 0010000000 ADRENAL, CORTEX (AC) .............. ................. NUMBER EXAMINED: 14 2 3 17 0 13 4 4 20 2 NOT REMARKABLE: 2 0 2 10 0 0 0 0 3 0 --ADRENAL TISSUE, ECTOPIC --ANGIECTASIS --ATROPHY, CORTICAL --CYST --DEGENERATION, CYSTIC, CORTICAL --HEMORRHAGE --HYPERPLASIA, CORTICAL --HYPERTROPHY, CORTICAL CELL --MINERALIZATION --NECROSIS, CORTICAL --PIGMENT 0001000000 4101072191 0000000011 0000001000 3111062371 6 2 0 1 0 13 4 4 16 2 300100002 0 2000000000 1000010011 0000000001 01 0 0 04 1 01 1 517 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M BER - O F - ANI M A LS- A F F E ' SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ..................... NUMBER EXAMINED: 14 2 3 17 0 13 4 4 20 2 NOT REMARKABLE: 2 0 2 10 0 0 0 0 3 0 --THROMBUS --VACUOLATION --B-ADENOMA --M-CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2 1 0 0 04 3 03 1 10 1 0 6 0 1 0 1 1 0 0000000100 0000000001 1000000000 ADRENAL, MEDULLA (MA) .................... NUMBER EXAMINED: 14 1 3 17 1 13 3 4 20 2 NOT REMARKABLE: 14 0 0 16 0 13 2 4 18 1 --NECROSIS --ONE EXAMINED --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 0010000000 0000000001 012100002 0 0010101000 LN, MESENTERIC (MS) ...................... NUMBER EXAMINED: 14 NOT REMARKABLE: 10 1 2 17 0 1 15 0 13 09 0 3 20 0 2 19 0 0 --DILATATION, SINUSOIDAL --EDEMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --NECROSIS --THROMBUS --M-HEMANGIOSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001000100 0000010000 0111010110 011002 0010 1000000000 1000000000 1100000000 1001000000 518 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 14 22 22 17 10 13 13 15 20 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- OVARY (OV) ........................ ................. NUMBER EXAMINED: 0 0 0 0 0 13 5 4 20 4 NOT REMARKABLE: 0 0 0 0 0 11 0 2 9 1 --CYST --HEMORRHAGE --MINERALIZATION --ONE EXAMINED --PIGMENTED --THROMBUS --B-GRANULOSA/THECA CELL TUMOR 0 0 0 0 02 52 8 3 0000001010 000000002 0 0000000001 000000002 0 000000002 0 0000000010 TESTIS (TE) ....................... ................. NUMBER EXAMINED: 14 22 22 17 10 0 0 0 0 0 NOT REMARKABLE: 12 16 18 10 9 0 0 0 0 0 --ATROPHY/DEGENERATION --HEMORRHAGE --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, VASCULAR --B-INTERSTITIAL CELL TUMOR 1333000000 0011000000 1310000000 0112 000000 0 0 02 1 0 0 0 0 0 1131000000 BONE, FEMUR (FE) .................. ................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 519 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 14 22 22 17 10 13 13 15 20 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) ................ ................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 12 0 0 20 0 --HEMORRHAGE 0000010000 MARROW, STERNUM (SE) .............. ................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 BONE, STERNUM (SB) ................ ................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 12 0 0 17 0 --HYPERPLASIA, CARTILAGE 0000010030 SPINAL CORD (SC) .................. ................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 THYMUS (TH) ....................... ................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 12 0 0 17 0 11 0 0 7 0 --CYST --M-THYMIC CARCINOMA 2 0 0 0 0 2 0 0 12 0 0000000010 LUNG (LU) ......................... ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 10 14 11 11 4 9 6 10 12 5 --FIBROSIS, PLEURAL/SUBPLEURAL 102 0210000 520 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 -- -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ........................... ............... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 10 14 11 11 4 9 6 10 12 5 --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --MINERALIZATION --MINERALIZATION, VASCULAR --NECROSIS --N-ADRENAL CORTICAL CARCINOMA --N-MALIGNANT PHEOCHROMOCYTOMA 1000000001 0002 002 001 2 02 002 0003 3111322153 0101100001 07734 04442 0000000001 0000000001 0000001000 HEART (HT) .......................... ............... NUMBER EXAMINED: 14 1 0 17 0 13 0 0 20 0 NOT REMARKABLE: 9 0 0 15 0 11 0 0 20 0 --DEGENERATION/NECROSIS --FIBROSIS 4102 02 0000 410002 0000 MUSCLE, SKELETAL (SM) ............... ............... NUMBER EXAMINED: 14 1 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 --DEGENERATION/NECROSIS 0100000000 NERVE, SCIATIC (SN) ................. ............... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 521 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R -O F -A N IM A L S -AFFE SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- NUMBER: 14 22 22 17 10 13 13 15 20 12 TRACHEA (TR) ........................................ NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 ESOPHAGUS (ES) .................. ................... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 THYROID (TY) ........................................ NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 6 8 9 4 2 7 6 8 8 3 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --NECROSIS --ONE EXAMINED --PIGMENT --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --M-CARCINOMA, C-CELL --M-CARCINOMA, FOLLICULAR CELL 1 1 34 2 1 1 02 1 2854232157 01 01 02 3 01 1 312 02 00021 0002 000001 0100000000 0001000000 0010000000 0000000010 1000000000 3666334445 02 1 3 1 0 02 1 0 0010000000 0010000000 522 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 14 22 22 17 10 13 13 15 20 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- PARATHYROID (PT) .................. ................. NUMBER EXAMINED: 14 2 0 15 0 13 0 0 20 0 NOT REMARKABLE: 12 1 0 15 0 12 0 0 20 0 --HYPERPLASIA --B-ADENOMA 2100000000 0000010000 PITUITARY (PI) .................... ................. NUMBER EXAMINED: 14 NOT REMARKABLE: 1 5 10 17 002 6 13 11 14 20 10 010050 --CYST --HEMORRHAGE --HYPERPLASIA --PIGMENT --B-ADENOMA 1 02 1 0 0 01 3 1 6 3 5 7 6 8 8 8 12 7 1000000000 0 0 0 0 02 2 12 1 12 5 10 15 6 12 11 14 15 10 HARDERIAN GLAND (HG) .............. ................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 13 0 0 13 0 11 0 0 18 0 --HEMORRHAGE --INFLAMMATION, CHRONIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010000 0004 02 002 0 1000000000 SALIV GL, MANDIB (SG) ............. ................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 523 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 14 22 22 17 10 13 13 15 20 12 ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- STOMACH, GL (ST) .................. ................. NUMBER EXAMINED: 14 3 1 17 1 13 0 0 20 5 NOT REMARKABLE: 12 1 0 15 0 12 0 0 19 5 --EDEMA --EROSION --FIBROSIS --MINERALIZATION --INFLAMMATION, CHRONIC-ACTIVE 1212110000 0011100010 0010000000 1100000000 0010000000 STOMACH, NONGL (SU) ............... ................. NUMBER EXAMINED: 14 1 1 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 12 0 0 20 0 --EDEMA --EROSION --INFLAMMATION, ACUTE --ULCERATION 0110010000 0010010000 0100000000 0100000000 DUODENUM (DU) ..................... ................. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 JEJUNUM (JE) .... .................................... NUMBER EXAMINED: 14 0 0 17 0 13 1 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 0000001000 524 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 14 22 22 17 10 13 13 15 20 12 ----------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ILEUM (IL) ................................... ...... NUMBER EXAMINED: 14 0 0 17 0 13 1 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 0000001000 CECUM (CE) ................................... ...... NUMBER EXAMINED: 14 0 1 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 1 17 0 12 0 0 20 0 --HEMORRHAGE 0000010000 COLON (CO) ................................... ...... NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 14 0 0 17 0 13 0 0 20 0 RECTUM (RE) ...................... .................. NUMBER EXAMINED: 14 0 0 16 0 13 0 0 20 0 NOT REMARKABLE: 11 0 0 12 0 13 0 0 18 0 --PARASITISM 3004 00002 0 SKIN (SK) ........................ .................. NUMBER EXAMINED: 14 7 5 17 2 12 2 1 20 0 NOT REMARKABLE: 12 1 0 13 0 10 1 1 17 0 --ACUTE INFLAMMATORY EXUDATE --CYST, EPIDERMAL INCLUSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC 0000100000 1010000000 0000000010 1011100010 022 001102 0 0000100000 525 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 -- -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ......................... ................. NUMBER EXAMINED: 14 7 5 17 2 12 2 1 20 0 NOT REMARKABLE: 12 1 0 13 0 10 1 1 17 0 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --B-FIBROMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL --M-SEBACEOUS GLAND CARCINOMA 0000010010 022 0211030 1110000000 0141000000 0102 000000 02 01000000 0100000000 0000001000 MAMMARY, FEMALE (MF) .............. ................. NUMBER EXAMINED: 0 0 0 0 0 12 13 15 20 12 NOT REMARKABLE: 0 0 0 0 0 1 2 3 7 1 --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --M-CARCINOMA 0 0 0 0 0 8 7 10 11 11 0000011211 000000022 0 0000011011 0000010100 0000001101 0 0 0 0 04 14 1 0 0000088574 0000012532 526 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 14 22 22 17 10 13 13 15 20 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- URINARY BLADDER (UB) ..................... .......... NUMBER EXAMINED: 14 22 22 17 10 13 13 14 20 12 NOT REMARKABLE: 13 10 12 16 7 11 13 14 20 10 --CALCULUS --DESQUAMATION, TRANSITIONAL EPITHELIUM --EDEMA --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --B-PAPILLOMA, TRANSITIONAL CELL --B-LEIOMYOMA --M-CARCINOMA, TRANSITIONAL CELL 0 10 8 0 3 0 0 0 0 0 0010000000 0010000000 0110000001 01 1 1 0 0 0 0 02 0110000000 1000000000 0000010000 0000010000 CERVIX (CV) .............................. .......... NUMBER EXAMINED: 0 0 0 0 0 13 2 1 20 2 NOT REMARKABLE: 0 0 0 0 0 11 0 0 18 0 --CYST --DILATATION --HYPERTROPHY, SMOOTH MUSCLE --PIGMENT --B-LEIOMYOMA --B-POLYP, ENDOMETRIAL STROMAL --M-LEIOMYOSARCOMA 0000010000 0000000010 0000001100 0000010000 0000000011 0000001010 0000000001 UTERUS (UT) .............................. .......... NUMBER EXAMINED: 0 0 0 0 0 13 4 5 20 4 NOT REMARKABLE: 0 0 0 0 0 4 0 0 9 0 --CYST, KERATIN 0000000010 527 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 -- -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) ......................................... NUMBER EXAMINED: 0 0 0 0 0 13 4 5 20 4 NOT REMARKABLE: 0 0 0 0 0 4 0 0 9 0 --DILATATION --HEMORRHAGE --HYPERPLASIA, CYSTIC, ENDOMETRIAL --HYPERPLASIA, ENDOMETRIAL --INFLAMMATION, CHRONIC --PIGMENT --B-LEIOMYOMA --B-POLYP, ENDOMETRIAL, STROMAL --M-CARCINOMA --M-LEIOMYOSARCOMA 0000073462 0000000100 0 0 0 0 03 1 04 3 0000010010 000002 0000 0000010000 0000010000 0000012 12 0 0000000100 0000000010 VAGINA (VA) ......................................... NUMBER EXAMINED: 0 0 0 0 0 13 0 1 19 1 NOT REMARKABLE: 0 0 0 0 0 12 0 1 19 0 --B-LEIOMYOMA --I-LEIOMYOSARCOMA 0000010000 0000000001 PROSTATE (PR) ....................................... NUMBER EXAMINED: 14 3 5 17 1 0 0 0 0 0 NOT REMARKABLE: 2 0 0 6 0 0 0 0 0 0 --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION, LUMINAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2 122 000000 2 0111 00000 7128000000 0102 000000 1000000000 528 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- NUMBER: 14 22 22 17 10 13 13 15 20 12 SEMINAL VESICLES (SV) .................... NUMBER EXAMINED: 14 0 0 17 0 0 0 0 0 0 NOT REMARKABLE: 12 0 0 16 0 0 0 0 0 0 --INFLAMMATION, CHRONIC-ACTIVE --SECRETION, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000000 1000000000 1000000000 EPIDIDYMIDES (EP) ........................ NUMBER EXAMINED: 14 0 0 17 0 0 0 0 0 0 NOT REMARKABLE: 12 0 0 15 0 0 0 0 0 0 --HYPOSPERMIA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1002 000000 1000000000 CAUSE OF DEATH (CD) ...................... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE --PITUITARY ADENOMA 14 22 22 17 10 13 13 15 19 12 0000000010 LN, MANDIBULAR (MN) ...................... NUMBER EXAMINED: 3 7 3 2 2 4 3 4 2 5 NOT REMARKABLE: 0 2 0 0 0 2 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01012 00102 2531122425 0001101000 0000001000 1000000000 529 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 14 22 22 17 10 13 13 15 20 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- LN, MEDIASTINAL (ML) ................. .............. NUMBER EXAMINED: 2 1 1 2 0 1 0 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 1112 000000 1000010010 0000000010 CAVITY, ABDOM (PC) ................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 1 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --NECROSIS, FAT 0000000101 CAVITY, ORAL (OC) .................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 HEMATO NEOPLASIA (HN) ................ .............. NUMBER EXAMINED: 14 0 0 17 0 13 0 0 20 0 NOT REMARKABLE: 13 0 0 16 0 13 0 0 20 0 --M-LYMPHOMA 1001000000 MESENTERY (MY) ....................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 530 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- NUMBER: 14 22 22 17 10 13 13 15 20 12 LN, ANT MES/PANC (AP) ............................... NUMBER EXAMINED: 0 1 1 0 0 0 1 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --N-ISLET CELL CARCINOMA 0010000000 0110001000 0000000010 0010000000 LN, LUMBAR/ILIAC (LM) ............................... NUMBER EXAMINED: 2 4 3 0 0 3 4 3 2 2 NOT REMARKABLE: 0 0 0 0 0 2 4 2 2 1 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 242 001 0000 0010000101 0010010000 LN, RENAL (RL) ...................................... NUMBER EXAMINED: 0 0 1 0 0 1 1 0 1 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE 0010011011 MAMMARY, MALE (MM) .................................. NUMBER EXAMINED: 3 1 0 3 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --GALACTOCELE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA 0101100000 0000100000 1000000000 1001000000 0001000000 531 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION NUMBER: 14 22 22 17 10 13 13 15 20 12 -- ---------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, MALE (MM) .................................. NUMBER EXAMINED: 3 1 0 3 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --B-FIBROADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2000000000 1000000000 LN, AXILLARY (AX) ................................... NUMBER EXAMINED: 0 3 0 3 0 3 4 6 6 3 NOT REMARKABLE: 0 2 0 2 0 1 3 3 2 3 --DILATATION, SINUSOIDAL --EDEMA --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 0000001100 0000000100 000101022 0 0100010010 000000002 0 CALVARIUM/SKULL (CA) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TAIL (TI) ............................ . ............. NUMBER EXAMINED: 0 1 0 1 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --EROSION --EXOSTOSES --INFLAMMATION, ACUTE 0100000001 0001000000 0100000001 532 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: -MALE- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- NUMBER: 14 22 22 17 10 13 13 15 20 12 ANUS (AN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 FOOT/FOOTPAD(S) (FP) ................................ NUMBER EXAMINED: 2 5 6 2 4 3 0 0 1 2 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --EDEMA --EROSION --EXOSTOSES --FIBROSIS --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE --PIGMENT 1260010000 2562330012 1421210000 134132 0002 2 5 6 14 3 0 0 02 0001000010 0000100000 LN, INGUINAL (IN) ................................... NUMBER EXAMINED: 0 0 1 0 0 1 1 1 1 0 NOT REMARKABLE: 0 0 1 0 0 0 1 1 0 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 0000000010 0000010010 JOINT, OTHER (JT) ................................... NUMBER EXAMINED: 0 1 1 0 0 1 0 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --EDEMA --EXOSTOSES --FIBROSIS --INFLAMMATION, ACUTE 0110000000 0110010100 0100010100 0110000000 533 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E T E D -- SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 14 22 22 17 10 13 13 15 20 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) ............... ................. NUMBER EXAMINED: 0 1 0 0 0 1 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --FIBROSIS --HEMORRHAGE --INFLAMMATION, CHRONIC --B-LIPOMA --I-TRANSITIONAL CELL CARCINOMA 0000001000 0000001000 0100000000 0100000000 0000010000 AORTA (AO) ........................ ................. NUMBER EXAMINED: 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 0 0 0 PENIS (PE) ........................ ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) .............. ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) .............. ................. NUMBER EXAMINED: 0 0 0 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --INFLAMMATION, ACUTE --M-CARCINOMA 0000100000 0000100000 534 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S - A F F E < T E D -- SEX: MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 14 22 22 17 10 13 13 15 20 12 -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- AORTIC ARCH (AA) .................. ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, TRACHEOBRON (TB) .............. ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 URETER (UE) ....................... ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 PINNA (PN) ........................ ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --HEMORRHAGE 0000000010 LN, POPLITEAL (PP) ................ ................. NUMBER EXAMINED: 1 2 0 0 0 0 0 1 0 0 NOT REMARKABLE: 0 1 0 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HYPERPLASIA, LYMPHOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000000100 0100000000 1000000000 CAVITY, THORACIC (TA) ............. ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 535 TABLE 43 Incidence of Microscopic Observations Terminal and Recovery Sacrifices 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E ' T E D -- SEX: -MALE-- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN AND FINDING DESCRIPTION -- NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ................ .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 TONGUE (TO) .......................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 LN, OTHER (LN) ....................... .............. NUMBER EXAMINED: 0 0 0 1 0 0 0 0 0 1 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 1 --DILATATION, SINUSOIDAL --INFILTRATE, MACROPHAGE, PIGMENTED 0001000000 0001000000 DIAPHRAGM (DP) ....................... .............. NUMBER EXAMINED: 1 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --B-FIBROMA 1000000000 BILE DUCT (BD) ....................... .............. NUMBER EXAMINED: 0 1 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 --DILATED ** END OF LIST ** 0100000000 536 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ** TOP OF LIST ** EYE (EY) .......................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 31 25 25 31 3 30 3 29 1 1 --FIBROSIS, CORNEAL --FIBROSIS, LENS --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CILIARY --INFLAMMATION, ACUTE, CORNEAL --MINERALIZATION, CORNEAL --MINERALIZATION, LENS --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0010000 0100000 3000000 0100000 0 02 1 01 0 2 011 000 1000000 1010000 0110010 0001000 BRAIN (BR) ........................ NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 10 12 8 11 3 30 3 11 1 1 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --NECROSIS, GRAY MATTER --PIGMENT --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 26 16 19 22 21 15 17 21 2211 0000 0010 0000 0 18 0 16 04 01 01 01 0 0 0 0 0 0 LIVER (LI) ........................ NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 14 13 8 4 3 30 06 1 0 --CYST 0002 000 537 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 14 13 8 4 3 30 06 1 0 --DEGENERATION, CYSTIC --FIBROSIS --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --STASIS, BILE --TELANGIECTASIS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --B-CHOLANGIOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3326060 0000010 0100000 52 01 01 0 1000000 0001000 212 0250 522704 0 0 0 02 2 2 1 8 6 7 11 0 6 0 0010000 0011000 1221331 0000010 0110000 13 8 11 16 0 10 0 12 12 000 0001000 1111010 LIVER, OIL RED O (LI1) ............ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 538 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 36 28 27 33 3 30 1 ---------------- -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) ......................................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 2 4 7 2 3 30 33 1 1 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NECROSIS --NECROSIS, PAPILLARY --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --B-LIPOMA --M-NEPHROBLASTOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 10 8 10 15 2100 7126 0100 0002 2200 7662 2 003 0100 1010 23 15 9 13 4126 0000 0100 1 0 02 08 00 03 00 00 01 09 01 00 01 0 15 06 01 00 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 SPLEEN (SP) ......................................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 35 28 25 26 3 30 3 27 1 1 --CYST, SEROSAL --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED --B-HEMANGIOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0013030 0012 000 0001000 1000000 539 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 36 28 27 33 3 30 1 ---------------- -=- -=- -=- -=- -=- -=- -=- PANCREAS (PA) ....................................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 27 19 18 24 3 30 3 25 1 1 --ATROPHY, ACINAR --FIBROSIS --HEMORRHAGE --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, VASCULAR --PIGMENT --B-ADENOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3242 02 0 0001000 1111000 0001000 0110010 1653030 32 03 01 0 1002 000 ADRENAL, CORTEX (AC) ................................ NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 17 16 18 17 3 30 3 21 1 1 --ANGIECTASIS --ATROPHY, CORTICAL --DEGENERATION, CYSTIC, CORTICAL --HEMORRHAGE --HYPERPLASIA, CORTICAL --HYPERTROPHY, CORTICAL CELL --MINERALIZATION --NECROSIS, CORTICAL --ONE EXAMINED --PIGMENT --THROMBUS --VACUOLATION --M-CARCINOMA 5104 04 0 1000000 525304 0 7217030 0100000 02 02 000 1000000 1000010 0001000 2 01 0000 0001000 11 9 6 11 0 4 0 1000000 540 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) .............. NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 17 16 18 17 3 30 3 21 1 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001000 ADRENAL, MEDULLA (MA) ............. NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 25 23 22 29 3 30 3 28 1 1 --HEMORRHAGE --HYPERPLASIA, MEDULLARY --ONE EXAMINED --VACUOLATION --B-PHEOCHROMOCYTOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2100000 1010000 0 02 1 0 0 0 0000010 9523010 1000000 LN, MESENTERIC (MS) ............... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 31 24 24 30 3 30 3 29 1 1 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0212 010 0001000 4220000 1100000 OVARY (OV) ........................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 541 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 36 28 27 33 3 30 1 TESTIS (TE) ....................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 30 22 21 28 --ATROPHY/DEGENERATION --GRANULOMA, SPERM --HEMORRHAGE --INFLAMMATION, CHRONIC --INFLAMMATION, VASCULAR --MINERALIZATION --MINERALIZATION, VASCULAR --NECROSIS --B-INTERSTITIAL CELL TUMOR 4332 0000 2000 1 1 02 1000 2 12 0 1112 0001 2000 BONE, FEMUR (FE) .................. NUMBER EXAMINED: 36 27 27 33 NOT REMARKABLE: 35 27 27 32 --OSTEODYSTROPHY, FIBROUS 1001 MARROW, FEMUR (FM) ................ NUMBER EXAMINED: 36 27 27 33 NOT REMARKABLE: 35 27 27 33 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000 MARROW, STERNUM (SE) .............. NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 35 28 27 33 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000 3 30 3 23 04 01 00 00 00 01 04 00 00 3 30 3 30 00 3 30 3 30 00 3 30 3 30 00 1 1 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 1 1 0 Covance 6329-212 3M T-6316.1 PAGE: 6 542 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 36 28 27 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- BONE, STERNUM (SB) .................................. NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 36 28 27 33 3 30 3 30 1 1 SPINAL CORD (SC) .................................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 36 28 26 33 --HEMORRHAGE --NECROSIS --VACUOLATION, WHITE MATTER 0000 0000 0010 THYMUS (TH) ......................................... NUMBER EXAMINED: 35 28 26 31 NOT REMARKABLE: 30 26 24 25 --CYST --DEPLETION, LYMPHOCYTIC --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3 1 02 0000 3122 1 1 02 LUNG (LU) ........................................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 19 19 12 20 --EDEMA, ALVEOLAR --FIBROSIS, PLEURAL/SUBPLEURAL --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC 1000 1301 1122 0000 1001 0010 3 29 3 27 02 01 00 3 30 0 25 00 01 34 00 3 30 2 14 01 00 13 02 02 00 1 1 0 0 0 1 1 0 0 0 0 1 1 0 0 0 0 0 0 543 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ** FROM PREVIOUS PAGE ** LUNG (LU) ................................ NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 19 19 12 20 3 30 2 14 1 1 --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --N-ADRENAL CORTICAL CARCINOMA 11 2 4 5 0 4 0 0001010 1010000 7486090 1013000 1000000 HEART (HT) ............................... NUMBER EXAMINED: 36 28 26 33 NOT REMARKABLE: 20 18 19 24 3 30 3 20 1 1 --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC --MINERALIZATION --OSSEOUS METAPLASIA --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 16 10 7 7 0 9 0 9633050 1000000 1001000 1000000 1000000 0001000 MUSCLE, SKELETAL (SM) .................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 33 28 26 30 3 30 3 27 1 1 --CYST --DEGENERATION/NECROSIS --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010 1012 02 0 1000000 1001000 Covance 6329-212 3M T-6316.1 PAGE: 8 544 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: -------------- MALE GROUP: -1- -2- -3- -4- -5- -6- -7NUMBER: 36 28 27 33 3 30 1 NERVE, SCIATIC (SN) ................................. NUMBER EXAMINED: 36 28 27 32 NOT REMARKABLE: 36 28 27 32 3 30 3 30 1 1 TRACHEA (TR) ................... .................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 36 28 27 33 3 30 3 30 1 1 ESOPHAGUS (ES) .................. ................... NUMBER EXAMINED: 36 28 24 33 NOT REMARKABLE: 36 28 24 33 3 30 3 30 1 1 THYROID (TY) ......................................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 22 13 16 19 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --ECTOPIC THYMUS --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, GRANULOMATOUS --INFLAMMATION, VASCULAR --MINERALIZATION --ONE EXAMINED --PIGMENT --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 1303 6763 0000 1100 02 00 0000 1000 0000 1000 0010 0010 7446 0103 3 30 2 21 02 13 00 01 01 01 00 01 00 00 00 02 00 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 545 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 -- -- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYROID (TY) ........................................ NUMBER EXAMINED: 36 28 27 33 3 30 1 NOT REMARKABLE: 22 13 16 19 2 21 0 --M-CARCINOMA, FOLLICULAR CELL 0000010 PARATHYROID (PT) .................................... NUMBER EXAMINED: 34 27 27 33 NOT REMARKABLE: 29 25 26 31 3 30 3 29 1 1 --HYPERPLASIA --B-ADENOMA 5112 01 0 0100000 PITUITARY (PI) ...................................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 4 4 2 2 3 30 35 1 1 --CYST --HEMORRHAGE --HYPERPLASIA --PIGMENT --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0101 23 15 20 22 0100 0010 32 23 25 30 0001 00 0 17 00 04 0 25 00 0 0 0 0 0 0 HARDERIAN GLAND (HG) ................................ NUMBER EXAMINED: 36 28 27 32 NOT REMARKABLE: 28 22 23 30 3 30 3 27 1 1 --INFLAMMATION, CHRONIC 8642 03 0 T E D -- 546 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 36 28 27 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- SALIV GL, MANDIB (SG) ............. ................. NUMBER EXAMINED: 36 28 27 32 NOT REMARKABLE: 34 28 27 32 3 30 3 30 1 1 --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 1000000 STOMACH, GL (ST) .................. ................. NUMBER EXAMINED: 36 28 27 32 NOT REMARKABLE: 28 28 22 24 3 30 3 27 1 0 --CYST --EDEMA --EROSION --HEMORRHAGE --MINERALIZATION --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --ULCERATION 0010000 102302 0 5024 021 0012 000 3011000 0 02 3 01 0 1011000 0001000 1001000 STOMACH, NONGL (SU) ............... ................. NUMBER EXAMINED: 36 28 27 32 NOT REMARKABLE: 30 22 23 27 3 30 3 25 1 1 --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION 563504 0 1523010 1002 000 1522 03 0 310302 0 2 002 030 Covance 6329-212 3M T-6316.1 PAGE: 11 T E D -- 547 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) ....... --B-PAPILLOMA, SQUAMOUS CELL DUODENUM (DU) ................ -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 36 28 27 33 3 30 1 NUMBER EXAMINED: 36 28 27 32 NOT REMARKABLE: 30 22 23 27 0010 NUMBER EXAMINED: 36 27 25 32 NOT REMARKABLE: 36 27 25 32 3 30 3 25 00 3 30 3 30 1 1 0 1 1 JEJUNUM (JE) NUMBER EXAMINED: 28 25 25 30 NOT REMARKABLE: 28 25 25 30 3 26 3 26 0 0 ILEUM (IL) --EDEMA CECUM (CE) --EDEMA --EROSION --HEMORRHAGE --PARASITISM NUMBER EXAMINED: 31 25 26 31 NOT REMARKABLE: 30 25 26 31 1000 NUMBER EXAMINED: 36 26 27 31 NOT REMARKABLE: 35 26 26 31 1000 1000 1000 0010 3 30 3 30 00 3 30 3 29 00 00 01 00 1 1 0 1 1 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 12 T E D -- 548 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 36 28 27 33 3 30 1 COLON (CO) ........................ NUMBER EXAMINED: 36 27 27 32 NOT REMARKABLE: 36 26 27 31 --PARASITISM 0101 RECTUM (RE) ....................... NUMBER EXAMINED: 36 27 26 33 NOT REMARKABLE: 34 26 26 32 --PARASITISM --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2100 0001 SKIN (SK) ......................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 34 22 26 29 --CYST, EPIDERMAL INCLUSION --EDEMA --HEMORRHAGE --INFLAMMATION, ACUTE --NECROSIS --B-HEMANGIOMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-SARCOMA, UNDIFFERENTIATED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1100 0100 0100 02 00 0100 0000 1110 0001 0101 0100 01 02 3 30 3 30 00 3 30 3 30 00 00 3 30 3 26 00 00 01 00 00 01 00 00 03 00 00 1 1 0 1 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 13 T E D -- 549 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: --MALE---- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 36 28 27 33 3 30 1 MAMMARY, FEMALE (MF) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 URINARY BLADDER (UB) ......... ................. NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 33 27 26 30 --CALCULUS --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001 0000 1112 1000 0111 1000 0000 0001 CERVIX (CV) ..... ................. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 3 30 3 28 00 02 00 01 01 00 01 00 00 00 1 1 0 0 0 0 0 0 0 0 0 0 UTERUS (UT) .................... .................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 VAGINA (VA) .................... .................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 14 A F F E C T E D -- 550 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 36 28 27 33 3 30 1 PROSTATE (PR) ..................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 8 7 4 6 --EDEMA --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION, LUMINAL --NECROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0111 2000 0001 7505 12 03 20 14 22 18 0022 0001 0001 SEMINAL VESICLES (SV) ............. NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 31 26 26 25 --EDEMA --FIBROSIS --HYPERPLASIA, EPITHELIAL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ONE EXAMINED --SECRETION, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001 0001 0001 1001 01 02 1001 1000 3113 0001 3 30 39 00 00 00 04 05 0 11 02 00 00 3 30 3 24 01 00 00 02 00 01 00 04 00 1 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 15 T E D -- 551 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 36 28 27 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- EPIDIDYMIDES (EP) ..................... ............. NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 35 26 25 33 3 30 3 28 1 1 --HYPOSPERMIA --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE 011002 0 0010000 1100000 CAUSE OF DEATH (CD) ................... ............. NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 0 0 0 0 3 30 00 1 0 --ADRENAL CORTICAL CARCINOMA --AUDITORY SEBACEOUS GLAND CARCINOMA --CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --FIBROSARCOMA --FRACTURE, HARD PALATE --HEMANGIOMA --HEPATIC NECROSIS --HEPATOCELLULAR ADENOMA --HISTIOCYTIC SARCOMA --MALIGNANT FIBROUS HISTIOSARCOMA --MALIGNANT LYMPHOMA --NECROSIS, ANAL MUSCULATURE --NEPHROBLASTOMA --PITUITARY ADENOMA --PYELONEPHRITIS --UNDETERMINED --UNDIFFERENTIATED SARCOMA 1000 0100 0010 2 1 02 1000 0100 0000 0000 02 1 1 0111 0001 1101 0000 0100 23 15 18 21 1010 7456 0100 00 00 00 00 00 00 01 21 00 00 00 01 01 00 0 21 00 14 00 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 16 T E D -- 552 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ** FROM PREVIOUS PAGE ** CAUSE OF DEATH (CD) .......... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 0 0 0 0 3 30 00 1 0 --UROLITHIASIS 0000010 LN, MANDIBULAR (MN) .......... NUMBER EXAMINED: 3 1 0 2 0 3 0 NOT REMARKABLE: 1 0 0 1 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001010 010002 0 0001000 1000000 LN, MEDIASTINAL (ML) ......... NUMBER EXAMINED: 2 0 2 1 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0010000 001102 0 102 002 0 1001000 CAVITY, ABDOM (PC) ........... NUMBER EXAMINED: 0 0 1 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --NECROSIS, FAT --M-CARCINOMA 0000010 0010000 T E D -- 553 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 36 28 27 33 3 30 1 -- -=- -=- -=- -=- -=- -=- -=- CAVITY, ORAL (OC) ................................... NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --BACTERIAL COLONIES --FRACTURE --HEMORRHAGE --INFLAMMATION, ACUTE 0100000 0100000 0100000 0100000 HEMATO NEOPLASIA (HN) ............................... NUMBER EXAMINED: 36 28 27 33 NOT REMARKABLE: 35 26 26 30 3 30 3 29 1 1 --M-LYMPHOMA --M-MALIGNANT FIBROUS HISTIOCYTOMA --M-SARCOMA, HISTIOCYTIC 1101010 0001000 0111000 MESENTERY (MY) ...................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE 1000000 LN, ANT MES/PANC (AP) ............................... NUMBER EXAMINED: 1 1 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0001000 1100000 T E D -- 554 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 36 28 27 33 3 30 1 T E D -- LN, LUMBAR/ILIAC (LM) ............. NUMBER EXAMINED: 2 5 1 4 0 1 0 NOT REMARKABLE: 0 2 0 0 0 1 0 --DILATATION, SINUSOIDAL --HYPERPLASIA, LYMPHOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 12 14 000 0112 000 1000000 LN, RENAL (RL) .................... NUMBER EXAMINED: 3 1 0 2 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 --DILATATION, SINUSOIDAL --FIBROSIS --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2101000 0001000 2000000 1001000 MAMMARY, MALE (MM) ................ NUMBER EXAMINED: 14 8 6 10 0 5 0 NOT REMARKABLE: 0 0 0 2 0 0 0 --GALACTOCELE --HEMORRHAGE --MINERALIZATION --PIGMENT --B-FIBROADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 12 5 4 4 0 2 0 1000000 12 00000 1223030 1100000 0001000 LN, AXILLARY (AX) ................. NUMBER EXAMINED: 2 0 0 0 0 1 0 NOT REMARKABLE: 2 0 0 0 0 1 0 555 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: --MALE---- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 36 28 27 33 3 30 1 CALVARIUM/SKULL (CA) ................................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TAIL (TI) ........................................... NUMBER EXAMINED: 2 3 1 2 0 3 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --NECROSIS --B-KERATOACANTHOMA 1211010 22 0001 0 1000000 0001000 22 01 01 0 0000010 0100000 ANUS (AN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --NECROSIS, MUSCULAR 0000010 FOOT/FOOTPAD(S) (FP) ................................ NUMBER EXAMINED: 9 2 6 5 0 6 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE 3032 02 0 626504 0 2 002 02 0 2263030 0001000 Covance 6329-212 3M T-6316.1 PAGE: 20 A F F E C T E D -- 556 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 36 28 27 33 3 30 1 ---- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** FOOT/FOOTPAD(S) (FP) ............ ................... NUMBER EXAMINED: 9 2 6 5 0 6 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE --PIGMENT 7255050 1010010 0000010 LN, INGUINAL (IN) ............... ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 JOINT, OTHER (JT) ................................... NUMBER EXAMINED: 1 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DISLOCATION --EDEMA --EXOSTOSES --HEMORRHAGE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE, SYNOVIAL 1000000 1000000 0001000 1000000 0001000 1000000 ADIPOSE TISSUE (AT) ................................. NUMBER EXAMINED: 1 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 0001000 Covance 6329-212 3M T-6316.1 PAGE: 21 T E D -- 557 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 36 28 27 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- AORTA (AO) ...................... ................... NUMBER EXAMINED: 2 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 2 01 0000 PENIS (PE) ...................... ................... NUMBER EXAMINED: 1 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --EDEMA --HEMORRHAGE --INFLAMMATION, CHRONIC ACTIVE --PROLAPSE 1000000 1000000 1000000 1100000 PREPUTIAL GLAND (PG) ............ ................... NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC 0100000 AUDITORY SEB GL (AS) ............ ................... NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --M-CARCINOMA 0100000 AORTIC ARCH (AA) ................ ................... NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 1000000 Covance 6329-212 3M T-6316.1 PAGE: 22 T E D -- 558 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 36 28 27 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) .............. ................. NUMBER EXAMINED: 1 0 0 2 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0001000 URETER (UE) ....................... ................. NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION --INFLAMMATION, CHRONIC 0000010 0000010 PINNA (PN) ........................ ................. NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 LN, POPLITEAL (PP) ................ ................. NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HYPERPLASIA, LYMPHOCYTIC 0010000 0010000 CAVITY, THORACIC (TA) ............. ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 23 T E D -- 559 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 36 28 27 33 3 30 1 -=- -=- -=- -=- -=- -=- -=- SUBCUTANEOUS TIS (SQ) ......... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TONGUE (TO) ................... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, OTHER (LN) ................ ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 DIAPHRAGM (DP) ................ ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 BILE DUCT (BD) ................ ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 24 A F F E C T E D -- 560 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 -- ------------------------------------ -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ........................ ................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 37 36 35 26 0 26 0 --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, CHRONIC-ACTIVE, --MINERALIZATION, CORNEAL --ONE EXAMINED --SYNECHIA, ANTERIOR --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL CORNEAL 0001000 0001000 0000010 0100000 0001000 0000010 0001000 000102 0 BRAIN (BR) ...................... ................... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 4 4 6 9 0 28 09 0 0 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --PIGMENT --VACUOLATION, WHITE MATTER --M-ASTROCYTOMA --I-PITUITARY CARCINOMA 32 32 29 20 22 24 21 13 4773 0010 0000 1000 0000 0 18 0 12 02 00 01 00 01 0 0 0 0 0 0 0 LIVER (LI) ...................... ................... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 18 15 19 9 0 28 0 11 0 0 --CYST --FIBROSIS 1001000 0310000 Covance 6329-212 3M T-6316.1 PAGE: 25 T E D -- 561 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 18 15 19 9 0 28 0 11 0 0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS, HEPATOCELLULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --PIGMENT --PIGMENT, HEPATOCELLULAR --PIGMENT, KUPFFER CELL --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 3301010 0010000 1103010 213002 0 3235000 6 10 6 8 0 7 0 0100000 1000000 4434 02 0 0001000 0100000 0000010 0001000 2410010 0000010 9 8 6 11 0 6 0 0 02 1 01 0 0000010 LIVER, OIL RED O (LI1) ............ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 26 T E D -- 562 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 37 37 35 29 0 28 0 ------------------------ -=- -=- -=- -=- -=- -=- -=- KIDNEY (KD) ................................. ....... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 4 1 8 10 0 28 03 0 0 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --B-LIPOMA 9 12 11 9 3001 4230 0101 0100 0000 23 24 18 14 7741 8622 1212 0000 08 00 02 00 01 01 0 19 03 03 02 01 0 0 0 0 0 0 0 0 0 0 0 SPLEEN (SP) ................................. ....... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 30 32 28 22 0 28 0 21 0 0 --FIBROSIS, CAPSULAR --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED --B-HEMANGIOMA 0100000 5226060 2151010 0100000 PANCREAS (PA) ............................... ....... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 31 35 31 25 0 28 0 26 0 0 --ATROPHY, ACINAR --HYPERPLASIA, ISLET CELLS 4123010 1000000 Covance 6329-212 3M T-6316.1 PAGE: 27 T E D -- 563 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 -- -- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PANCREAS (PA) ..................... ................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 31 35 31 25 0 26 0 --PIGMENT --B-ADENOMA, ISLET CELL --I-ENDOMETRIAL STROMAL SARCOMA 2 021000 0000010 0100000 ADRENAL, CORTEX (AC) .............. ................. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 5 3 2 14 0 28 03 0 0 --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --FIBROSIS --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY, CORTICAL CELL --MINERALIZATION --PIGMENT --THROMBUS --VACUOLATION --B-ADENOMA 25 30 23 9 11 15 16 5 0001 0000 31 33 30 10 2 02 0 0100 3341 2321 3224 2 022 0 15 0 13 00 01 0 18 00 01 01 03 01 01 0 0 0 0 0 0 0 0 0 0 0 ADRENAL, MEDULLA (MA) ............. ................. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 35 34 27 0 28 0 27 0 0 --ONE EXAMINED --VACUOLATION --B-PHEOCHROMOCYTOMA 0001000 0001010 02 1 0 01 0 Covance 6329-212 3M T-6316.1 PAGE: 28 T E D -- 564 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 37 37 35 29 0 28 0 -=- -=- -=- -=- -=- -=- -=- LN, MESENTERIC (MS) .................. .............. NUMBER EXAMINED: 36 36 35 28 NOT REMARKABLE: 33 34 29 26 0 28 0 25 0 0 --DILATATION, SINUSOIDAL --EDEMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 0002 000 0010000 1011010 326002 0 OVARY (OV) ........................... .............. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 26 25 26 16 0 28 0 21 0 0 --CYST --HEMORRHAGE --MINERALIZATION --ONE EXAMINED --PIGMENTED 7898070 12 02 000 22 01 000 1102 000 0001000 TESTIS (TE) .......................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 BONE, FEMUR (FE) ................. .................. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 37 35 29 0 27 0 27 0 0 MARROW, FEMUR (FM) ................ ................. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 37 35 29 0 27 0 27 0 0 Covance 6329-212 3M T-6316.1 PAGE: 29 T E D -- 565 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 37 37 35 29 0 28 0 MARROW, STERNUM (SE) NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 37 35 29 0 28 0 28 0 0 BONE, STERNUM (SB) NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 37 35 29 0 28 0 28 0 0 SPINAL CORD (SC) NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 37 34 29 --HEMORRHAGE 0010 THYMUS (TH) ... NUMBER EXAMINED: 37 36 35 28 NOT REMARKABLE: 23 27 24 17 --CYST --DEPLETION, LYMPHOCYTIC --HEMORRHAGE --M-THYMIC CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 10 7 10 9 1010 32 00 0001 1001 LUNG (LU) ........................................... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 28 29 26 18 --EDEMA, ALVEOLAR --FOREIGN BODY --HEMORRHAGE 0100 0010 012 0 0 28 0 27 01 0 25 0 19 05 00 01 00 00 0 28 0 14 01 00 03 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 30 A F F E C T E D -- 566 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 -- ------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ............................ .............. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 28 29 26 18 0 14 0 --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --INFLAMMATION, GRANULOMATOUS --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --MINERALIZATION, VASCULAR --THROMBUS --N-MAMMARY CARCINOMA 0002 02 0 0110000 0010000 5 04 5 06 0 0010000 02 01030 6423060 0001000 0001010 HEART (HT) ........................... .............. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 30 32 30 27 0 28 0 28 0 0 --DEGENERATION/NECROSIS --FIBROSIS --MINERALIZATION 7542 000 3132 000 0011000 MUSCLE, SKELETAL (SM) ................ .............. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 36 37 34 29 0 28 0 28 0 0 --DEGENERATION/NECROSIS 1010000 NERVE, SCIATIC (SN) .................. .............. NUMBER EXAMINED: 36 37 35 29 NOT REMARKABLE: 36 37 35 29 0 28 0 28 0 0 Covance 6329-212 3M T-6316.1 PAGE: 31 T E D -- 567 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 37 37 35 29 0 28 0 -=- -=- -=- -=- -=- -=- -=- TRACHEA (TR) ........................................ NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 37 35 29 0 28 0 28 0 0 ESOPHAGUS (ES) .................. ................... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 37 35 29 0 28 0 28 0 0 THYROID (TY) ............................. .......... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 23 19 26 19 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HEMORRHAGE --HYPERPLASIA, C-CELL --INFLAMMATION, CHRONIC --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --M-CARCINOMA, C-CELL 1101 8962 0311 1010 0000 5 94 6 1 0 02 1000 PARATHYROID (PT) ......................... .......... NUMBER EXAMINED: 34 34 34 28 NOT REMARKABLE: 34 34 34 28 0 28 0 22 00 02 00 00 01 03 01 00 0 27 0 27 0 0 0 0 0 0 0 0 0 0 0 0 PITUITARY (PI) .................. ................... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 3 1 1 2 --CYST 2442 0 28 02 00 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 32 A F F E C T E D -- 568 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ---- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PITUITARY (PI) ................ ..................... NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 3 1 1 2 0 2 0 --HEMORRHAGE --HYPERPLASIA --NECROSIS --PIGMENT --B-ADENOMA --M-CARCINOMA 25 29 30 17 0000 0100 3675 34 35 34 26 0000 0 18 01 00 03 0 24 01 0 0 0 0 0 0 HARDERIAN GLAND (HG) .......... ..................... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 28 31 27 24 0 28 0 26 0 0 --INFLAMMATION, CHRONIC --ONE EXAMINED 868502 0 1000000 SALIV GL, MANDIB (SG) ......... ..................... NUMBER EXAMINED: 37 37 35 28 NOT REMARKABLE: 37 37 35 28 0 27 0 27 0 0 STOMACH, GL (ST) ..................... ............... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 35 36 32 24 --CYST --EDEMA --EROSION --MINERALIZATION --INFLAMMATION, ACUTE 0000 1013 1122 0011 0010 0 28 0 27 01 00 00 00 00 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 33 T E D -- 569 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 34 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: ------------- FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7NUMBER: 37 37 35 29 0 28 0 T E D -- STOMACH, NONGL (SU) --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR MUCOSA --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION DUODENUM (DU) ......................... NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 30 32 32 25 6534 1 3 02 1000 1 0 02 1000 24 04 3000 2 002 NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 37 35 29 0 28 0 23 04 02 00 00 00 02 00 01 0 28 0 28 0 0 0 0 0 0 0 0 0 0 0 0 JEJUNUM (JE) --CYST, KERATIN --DIVERTICULUM --B-ANGIOMA ILEUM (IL) ...... NUMBER EXAMINED: 36 37 33 28 NOT REMARKABLE: 35 36 33 28 0000 0100 1000 NUMBER EXAMINED: 34 36 34 29 NOT REMARKABLE: 34 36 34 29 0 26 0 25 01 00 00 0 27 0 27 0 0 0 0 0 0 0 570 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 37 37 35 29 0 28 0 ----------------------------------- -=- -=- -=- -=- -=- -=- -=- CECUM (CE) ....................... .................. NUMBER EXAMINED: 36 37 35 29 NOT REMARKABLE: 35 37 35 29 0 28 0 28 0 0 --PARASITISM 1000000 COLON (CO) ....................... .................. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 36 37 35 28 0 28 0 28 0 0 --EDEMA --PARASITISM 0001000 1000000 RECTUM (RE) ...................... .................. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 36 35 32 29 0 27 0 27 0 0 --PARASITISM 1230000 SKIN (SK) ........................ .................. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 32 33 27 25 0 28 0 22 0 0 --ACUTE INFLAMMATORY EXUDATE --ATROPHY, ADNEXAL --CYST, EPIDERMAL INCLUSION --EDEMA --FAT NECROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE 0100000 0001010 1000000 0010010 1000000 1111000 323102 0 0011000 000102 0 Covance 6329-212 3M T-6316.1 PAGE: 35 T E D -- 571 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 -- ---------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ......................... ................. NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 32 33 27 25 0 22 0 --ULCERATION --B-FIBROMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3242 05 0 0010000 0010000 1000000 0000010 0110000 0000010 MAMMARY, FEMALE (MF) .............. ................. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 5 5 7 7 0 28 04 0 0 --EDEMA --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --M-CARCINOMA --M-FIBROSARCOMA 0000 23 25 24 16 4357 1000 0143 0010 0010 5785 1232 3253 2322 14 13 11 6 8 7 10 7 1000 01 0 16 02 00 00 00 01 03 00 00 04 08 04 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 36 T E D -- 572 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 -- -- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MAMMARY, FEMALE (MF) ................................ NUMBER EXAMINED: 37 37 35 29 0 28 0 NOT REMARKABLE: 5 5 7 7 0 4 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010 URINARY BLADDER (UB) ................................ NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 37 37 35 27 0 28 0 28 0 0 --INFLAMMATION, CHRONIC 0002 000 CERVIX (CV) ......................................... NUMBER EXAMINED: 37 37 34 29 NOT REMARKABLE: 36 35 33 29 0 27 0 24 0 0 --DILATATION --HEMORRHAGE --INFLAMMATION, ACUTE --NECROSIS --B-POLYP, ENDOMETRIAL STROMAL --M-SARCOMA, ENDOMETRIAL STROMAL 1100010 0100010 0000010 0100000 1100030 0110000 UTERUS (UT) ......................................... NUMBER EXAMINED: 37 37 34 29 NOT REMARKABLE: 30 27 28 21 0 28 0 22 0 0 --DILATATION --HEMORRHAGE --HYPERPLASIA, CYSTIC, ENDOMETRIAL --INFLAMMATION, ACUTE --NECROSIS 6845050 0002 010 0212 010 0011010 0000010 Covance 6329-212 3M T-6316.1 PAGE: 37 T E D -- 573 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 37 37 35 29 0 28 0 ---- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** UTERUS (UT) ...................... .................. NUMBER EXAMINED: 37 37 34 29 0 28 0 NOT REMARKABLE: 30 27 28 21 0 22 0 --PIGMENT --B-POLYP, ENDOMETRIAL, STROMAL --M-HEMANGIOSARCOMA 0001000 1011010 1000000 VAGINA (VA) ...................... .................. NUMBER EXAMINED: 37 37 35 28 NOT REMARKABLE: 37 35 33 28 0 27 0 27 0 0 --HEMORRHAGE --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL --I-ENDOMETRIAL STROMAL SARCOMA 0100000 0100000 0010000 0010000 PROSTATE (PR) .................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) ............... ............... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 EPIDIDYMIDES (EP) ................. ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 38 T E D -- 574 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 37 37 35 29 0 28 0 -=- -=- -=- -=- -=- -=- -=- CAUSE OF DEATH (CD) ................................. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 0 0 0 0 0 28 00 0 0 --ASTROCYTOMA --C-CELL CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --ENDOMETRIAL STROMAL POLYP --ENDOMETRIAL STROMAL SARCOMA --FIBROMA --FIBROSARCOMA --HEMANGIOSARCOMA --HEPATOCELLULAR ADENOMA --KERATIN CYST --KERATOACANTHOMA --LIPOMA --LIPOSARCOMA --MALIGNANT FIBROUS HISTIOSARCOMA --MALIGNANT LYMPHOMA --MAMMARY CARCINOMA --MAMMARY FIBROADENOMA --PITUITARY ADENOMA --PITUITARY CARCINOMA --RENAL MINERALIZATION --SQUAMOUS CELL CARCINOMA --THYMIC CARCINOMA --UNDETERMINED --UTERINE HEMORRHAGE 1000 1000 0000 0000 0110 0010 0110 1000 0010 0000 0010 1000 0010 0000 1000 0363 54 01 27 28 22 16 0001 0001 0010 0001 0005 0001 00 00 01 02 00 00 00 00 00 01 00 00 00 01 00 02 01 0 18 01 00 00 00 01 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 39 T E D -- 575 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 40 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION --------------------------------------- -- NUMBER: 37 37 35 29 0 28 0 -=- -=- -=- -=- -=- -=- -=- LN, MANDIBULAR (MN) .................. .............. NUMBER EXAMINED: 4 3 5 7 0 5 0 NOT REMARKABLE: 2 2 3 4 0 3 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 1 012 000 111102 0 LN, MEDIASTINAL (ML) ................. .............. NUMBER EXAMINED: 2 2 5 3 0 4 0 NOT REMARKABLE: 0 0 1 0 0 1 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 1211030 214302 0 CAVITY, ABDOM (PC) ................... .............. NUMBER EXAMINED: 0 4 0 2 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE --INFLAMMATION, CHRONIC --MINERALIZATION --NECROSIS, FAT --PIGMENT --THROMBUS --I-ENDOMETRIAL STROMAL SARCOMA 0101000 0001000 0101000 0302 010 0001000 0001000 0100000 CAVITY, ORAL (OC) .................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 T E D -- 576 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 41 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: ------------- FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7NUMBER: 37 37 35 29 0 28 0 T E D -- HEMATO NEOPLASIA (HN) ................ .............. NUMBER EXAMINED: 37 37 35 29 NOT REMARKABLE: 36 37 35 28 --M-LYMPHOMA --M-MALIGNANT FIBROUS HISTIOCYTOMA 1001 0000 MESENTERY (MY) .. .............. NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 28 0 27 00 01 00 00 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ................ .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, LUMBAR/ILIAC (LM) ................ .............. NUMBER EXAMINED: 2 2 2 1 0 4 0 NOT REMARKABLE: 1 1 1 1 0 1 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --N-ENDOMETRIAL STROMAL SARCOMA 1000010 1100010 000002 0 0010000 LN, RENAL (RL) .. .............. NUMBER EXAMINED: 0 1 0 1 0 1 0 NOT REMARKABLE: 0 1 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 0001000 0000010 0001000 577 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 37 37 35 29 0 28 0 MAMMARY, MALE (MM) NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, AXILLARY (AX) NUMBER EXAMINED: 12 13 12 6 0 3 0 NOT REMARKABLE: 8 9 8 2 0 1 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 3413000 1 02 1 01 0 1111000 0000010 CALVARIUM/SKULL (CA) ................................ NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 1 0 0 0 0 0 TAIL (TI) NUMBER EXAMINED: 0 0 1 2 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EROSION --INFLAMMATION, ACUTE 0002 000 0010000 0012 000 ANUS (AN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 42 T E D -- 578 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 37 37 35 29 0 28 0 -=- -=- -=- -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) .............. ................. NUMBER EXAMINED: 4 3 3 0 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE 0100000 1100010 42 0001 0 1100000 3230010 0100000 432 002 0 0010000 LN, INGUINAL (IN) ................. ................. NUMBER EXAMINED: 3 3 3 1 0 0 0 NOT REMARKABLE: 1 2 2 0 0 0 0 --HYPERPLASIA, LYMPHOCYTIC --N-MAMMARY CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1110000 0001000 1000000 JOINT, OTHER (JT) ................. ................. NUMBER EXAMINED: 2 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --EXOSTOSES --INFLAMMATION, CHRONIC 2000000 2000000 ADIPOSE TISSUE (AT) ............... ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 43 T E D -- 579 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 37 37 35 29 0 28 0 -=- -=- -=- -=- -=- -=- -=- AORTA (AO) .................... ..................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 0000010 PENIS (PE) .................... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) .......... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) .......... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 AORTIC ARCH (AA) .............. ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, TRACHEOBRON (TB) .......... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 URETER (UE) ................... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 44 T E D -- 580 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 37 37 35 29 0 28 0 -------------------------------------- -=- -=- -=- -=- -=- -=- -=- PINNA (PN) .................... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, POPLITEAL (PP) ............ ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 CAVITY, THORACIC (TA) ......... ..................... NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --M-LIPOSARCOMA 0010000 SUBCUTANEOUS TIS (SQ) ......... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TONGUE (TO) ................... ..................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST 0000010 LN, OTHER (LN) ................ ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 45 A F F E C T E D -- 581 TABLE 44 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL N U M BE R -O F -A N IMA SEX: -FEMALE--- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 37 37 35 29 0 28 0 -=- -=- -=- -=- -=- -=- -=- DIAPHRAGM (DP) ...................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 BILE DUCT (BD) .................. ................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 46 A F F E C T E D -- 582 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ** TOP OF LIST ** EYE (EY) .......................... NUMBER EXAMINED: 65 34 33 65 NOT REMARKABLE: 58 30 31 62 8 30 8 29 1 1 --CATARACT --FIBROSIS, CORNEAL --FIBROSIS, LENS --HYPERPLASIA, CORNEAL EPITHELIUM --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CILIARY --INFLAMMATION, ACUTE, CORNEAL --MINERALIZATION, CORNEAL --MINERALIZATION, LENS --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 0010000 0100000 1000000 3000000 02 00000 1122 01 0 3011000 1000000 1110000 02 1 0 01 0 0001000 BRAIN (BR) ........................ NUMBER EXAMINED: 65 37 36 65 NOT REMARKABLE: 37 17 15 39 8 34 8 11 1 1 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --NECROSIS, GRAY MATTER --PIGMENT --B-GRANULAR CELL TUMOR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 28 20 21 25 21 16 17 22 2211 0000 0010 0001 0000 0 22 0 16 04 01 01 00 01 0 0 0 0 0 0 0 583 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 LIVER (LI) .......................................... NUMBER EXAMINED: 65 55 55 65 NOT REMARKABLE: 26 17 10 5 --CYST --DEGENERATION, CYSTIC --FIBROSIS --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --PIGMENT --STASIS, BILE --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --B-CHOLANGIOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1 0 02 6 8 14 18 0010 0100 1110 8 12 11 6 1 02 1 1001 3331 9 9 8 11 0 1 3 22 0000 18 20 23 20 0010 02 12 1342 0111 0001 2730 0100 17 18 23 39 2425 0001 2111 8 40 08 00 07 02 00 00 02 00 00 57 05 72 01 09 00 00 64 01 01 00 00 1 11 00 00 01 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 584 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 -------------------------------- -=- -=- -=- -=- -=- -=- -=- LIVER, OIL RED O (LI1) .............. ............... NUMBER EXAMINED: 5 0 0 5 0 0 0 NOT REMARKABLE: 1 0 0 1 0 0 0 --POSITIVE OIL RED O STAINING 4 004 000 KIDNEY (KD) ......................... ............... NUMBER EXAMINED: 65 55 55 65 NOT REMARKABLE: 8 7 13 20 8 40 73 1 1 --CAST, PROTEINACEOUS --CYST --DEGENERATION, TUBULAR --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NECROSIS --NECROSIS, PAPILLARY --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --THROMBUS --B-LIPOMA --M-LIPOSARCOMA --M-NEPHROBLASTOMA 17 15 16 18 4530 0100 8267 0100 1 0 02 2410 1100 0101 1000 9 8 10 4 2 003 0100 1010 37 29 24 22 5226 1000 0000 0000 0100 0 11 10 00 05 00 00 02 00 00 00 09 01 00 01 1 22 06 00 01 01 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 585 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ** FROM PREVIOUS PAGE ** KIDNEY (KD) .............................. NUMBER EXAMINED: 65 55 55 65 NOT REMARKABLE: 8 7 13 20 8 40 73 1 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1002 000 SPLEEN (SP) .............................. NUMBER EXAMINED: 65 34 33 65 NOT REMARKABLE: 63 34 31 58 8 30 8 27 1 1 --CYST, SEROSAL --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED --B-HEMANGIOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0013030 0012 000 0001000 2000000 PANCREAS (PA) ............................ NUMBER EXAMINED: 65 55 55 65 NOT REMARKABLE: 45 32 36 45 8 40 8 28 1 1 --ATROPHY, ACINAR --CYST --FIBROSIS --HEMORRHAGE --HYPERPLASIA, ACINAR --HYPERTROPHY, VASCULAR --INFILTRATE, EOSINOPHILS --INFILTRATION, FAT, INTERSTITIAL --INFLAMMATION, CHRONIC --INFLAMMATION, VASCULAR --PIGMENT 10 9 10 9 0 7 0 0100000 1002 000 3211000 12 00000 2 00001 0 1000000 0001000 2 001 000 231002 0 2896050 586 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ** FROM PREVIOUS PAGE ** PANCREAS (PA) ..................... NUMBER EXAMINED: 65 55 55 65 NOT REMARKABLE: 45 32 36 45 8 40 8 28 1 1 --B-ADENOMA, ISLET CELL --M-CARCINOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 472602 0 0010000 1002 000 ADRENAL, CORTEX (AC) .............. NUMBER EXAMINED: 65 35 36 65 NOT REMARKABLE: 30 18 25 40 8 30 8 21 1 1 --ADRENAL TISSUE, ECTOPIC --ANGIECTASIS --ATROPHY, CORTICAL --DEGENERATION, CYSTIC, CORTICAL --HEMORRHAGE --HYPERPLASIA, CORTICAL --HYPERTROPHY, CORTICAL CELL --MINERALIZATION --NECROSIS, CORTICAL --ONE EXAMINED --PIGMENT --THROMBUS --VACUOLATION --M-CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 92 0504 0 1000000 9364 04 0 14 4 1 8 0 3 0 3101000 32 02 000 2000000 1000010 0001000 2110000 2101000 24 13 7 19 0 4 0 1000000 2 001 000 587 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 ADRENAL, MEDULLA (MA) .................... NUMBER EXAMINED: 65 34 36 65 NOT REMARKABLE: 54 28 28 59 --HEMORRHAGE --HYPERPLASIA, MEDULLARY --NECROSIS --ONE EXAMINED --VACUOLATION --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2101 1010 0010 0 02 1 0000 9645 0010 1000 LN, MESENTERIC (MS) ...................... NUMBER EXAMINED: 65 34 35 65 NOT REMARKABLE: 55 29 30 59 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --NECROSIS --THROMBUS --M-HEMANGIOSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001 1334 0001 4331 1000 1000 1100 2101 OVARY (OV) ............................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 8 31 8 28 00 00 00 00 01 01 01 00 8 30 8 29 00 01 00 00 00 00 00 00 00 00 1 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 588 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 TESTIS (TE) ....................... NUMBER EXAMINED: 65 55 55 65 NOT REMARKABLE: 57 43 44 52 --ATROPHY/DEGENERATION --GRANULOMA, SPERM --HEMORRHAGE --INFLAMMATION, CHRONIC --INFLAMMATION, VASCULAR --MINERALIZATION --MINERALIZATION, VASCULAR --NECROSIS --B-INTERSTITIAL CELL TUMOR 5676 0000 2 011 2412 1000 2232 1114 0001 3131 BONE, FEMUR (FE) .................. NUMBER EXAMINED: 55 27 28 60 NOT REMARKABLE: 54 27 28 59 --OSTEODYSTROPHY, FIBROUS 1001 MARROW, FEMUR (FM) ................ NUMBER EXAMINED: 55 27 28 60 NOT REMARKABLE: 54 27 28 60 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000 MARROW, STERNUM (SE) .............. NUMBER EXAMINED: 55 28 28 60 NOT REMARKABLE: 54 28 28 60 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000 8 40 8 32 04 01 00 00 00 01 05 00 00 3 30 3 30 00 3 30 3 30 00 3 30 3 30 00 1 1 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 1 1 0 Covance 6329-212 3M T-6316.1 PAGE: 7 589 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 BONE, STERNUM (SB) NUMBER EXAMINED: 55 28 28 60 NOT REMARKABLE: 55 28 28 60 3 30 3 30 1 1 SPINAL CORD (SC) NUMBER EXAMINED: 55 28 28 60 NOT REMARKABLE: 55 28 27 60 --HEMORRHAGE --NECROSIS --VACUOLATION, WHITE MATTER 0000 0000 0010 THYMUS (TH) ................. NUMBER EXAMINED: 55 28 27 57 NOT REMARKABLE: 47 26 25 51 --CYST --DEPLETION, LYMPHOCYTIC --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 5 1 02 0000 4122 1 1 02 LUNG (LU) ........................................... NUMBER EXAMINED: 55 50 50 60 NOT REMARKABLE: 33 33 23 38 --EDEMA, ALVEOLAR --FIBROSIS, PLEURAL/SUBPLEURAL --HEMORRHAGE --HYPERPLASIA, MEDIAL, ARTERIAL/ARTERIOLAR --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, ACUTE 1000 2321 2132 0001 0002 1001 3 29 3 27 02 01 00 3 30 0 25 00 01 34 00 3 40 2 18 01 02 13 00 02 02 1 1 0 0 0 1 1 0 0 0 0 1 1 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 8 A F F E C T E D -- 590 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ** FROM PREVIOUS PAGE ** LUNG (LU) ................................ NUMBER EXAMINED: 55 50 50 60 NOT REMARKABLE: 33 33 23 38 3 40 2 18 1 1 --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --N-ADRENAL CORTICAL CARCINOMA 2 03 0 15 3 5 7 0001 1111 8 11 15 10 1013 1000 00 07 01 01 0 13 00 00 0 0 0 0 0 0 0 HEART (HT) ............................... NUMBER EXAMINED: 55 29 27 60 NOT REMARKABLE: 34 18 20 48 3 30 3 20 1 1 --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC --MINERALIZATION --OSSEOUS METAPLASIA --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 20 11 7 10 0 9 0 13 7 3 3 0 5 0 1000000 1001000 1000000 1000000 0001000 MUSCLE, SKELETAL (SM) .................... NUMBER EXAMINED: 55 29 28 60 NOT REMARKABLE: 52 28 26 57 3 30 3 27 1 1 --CYST --DEGENERATION/NECROSIS --HEMORRHAGE 0000010 1112 02 0 0010000 Covance 6329-212 3M T-6316.1 PAGE: 9 591 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- ---------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** MUSCLE, SKELETAL (SM) ............. ................. NUMBER EXAMINED: 55 29 28 60 3 30 1 NOT REMARKABLE: 52 28 26 57 3 27 1 --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000000 1001000 NERVE, SCIATIC (SN) ............... ................. NUMBER EXAMINED: 55 28 28 59 NOT REMARKABLE: 55 28 28 59 3 30 3 30 1 1 TRACHEA (TR) ................... .................... NUMBER EXAMINED: 55 28 28 60 NOT REMARKABLE: 55 28 28 60 3 30 3 30 1 1 ESOPHAGUS (ES) .................. ................... NUMBER EXAMINED: 55 28 25 60 NOT REMARKABLE: 55 28 25 60 3 30 3 30 1 1 THYROID (TY) .............................. ......... NUMBER EXAMINED: 55 50 50 60 NOT REMARKABLE: 31 21 25 30 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --ECTOPIC THYMUS --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, GRANULOMATOUS 244 10 15 11 000 12 0 332 000 110 8 9 0 1 0 2 0 3 40 2 23 04 15 00 01 03 01 00 1 0 0 0 1 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 10 A F F E C T E D -- 592 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- ---------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYROID (TY) ...................... ................. NUMBER EXAMINED: 55 50 50 60 3 40 1 NOT REMARKABLE: 31 21 25 30 2 23 0 --INFLAMMATION, VASCULAR --MINERALIZATION --NECROSIS --ONE EXAMINED --PIGMENT --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL --M-CARCINOMA, C-CELL --M-CARCINOMA, FOLLICULAR CELL 0000010 1001000 0010000 0010000 1010000 10 10 10 12 0 5 0 0316010 0010000 0010010 PARATHYROID (PT) .................. ................. NUMBER EXAMINED: 54 29 27 58 NOT REMARKABLE: 46 26 26 56 3 30 3 29 1 1 --HYPERPLASIA --B-ADENOMA 82 12 01 0 0100000 PITUITARY (PI) .................... ................. NUMBER EXAMINED: 55 33 38 60 NOT REMARKABLE: 8 4 2 8 3 36 35 1 1 --CYST --HEMORRHAGE --HYPERPLASIA --PIGMENT --B-ADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1122 29 18 25 31 1103 0010 46 28 36 48 0001 00 0 23 00 04 0 31 00 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 11 T E D -- 593 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 HARDERIAN GLAND (HG) .............. NUMBER EXAMINED: 55 28 28 59 NOT REMARKABLE: 46 22 24 53 --INFLAMMATION, CHRONIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 8646 1000 SALIV GL, MANDIB (SG) ............. NUMBER EXAMINED: 55 28 28 59 NOT REMARKABLE: 53 28 28 59 --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1000 1000 STOMACH, GL (ST) .................. NUMBER EXAMINED: 57 31 29 59 NOT REMARKABLE: 46 29 23 48 --CYST --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --MINERALIZATION --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --ULCERATION 0010 3235 5036 0010 0012 4111 0 02 3 1 02 1 0001 1001 3 30 3 27 03 00 3 30 3 30 00 00 3 31 3 27 00 03 03 00 00 00 01 00 00 00 1 1 0 0 1 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 12 T E D -- 594 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- STOMACH, NONGL (SU) ............. ................... NUMBER EXAMINED: 57 29 29 59 NOT REMARKABLE: 50 22 24 54 3 30 3 25 1 1 --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --B-PAPILLOMA, SQUAMOUS CELL 674504 0 2533010 2 002 000 1622 03 0 310302 0 2102 030 0010000 DUODENUM (DU) ................... ................... NUMBER EXAMINED: 59 27 26 59 NOT REMARKABLE: 59 27 26 59 4 30 4 30 1 1 JEJUNUM (JE) ................... .................... NUMBER EXAMINED: 48 25 26 57 NOT REMARKABLE: 48 25 26 57 3 26 3 26 0 0 ILEUM (IL) ..................... .................... NUMBER EXAMINED: 50 25 27 58 NOT REMARKABLE: 49 25 27 58 --EDEMA 1000 CECUM (CE) ..................... .................... NUMBER EXAMINED: 55 26 29 58 NOT REMARKABLE: 54 26 28 58 --EDEMA 1000 3 30 3 30 00 3 30 3 29 00 1 1 0 1 1 0 Covance 6329-212 3M T-6316.1 PAGE: 13 A F F E C T E D -- 595 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- ---------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CECUM (CE) ........................ ................. NUMBER EXAMINED: 55 26 29 58 3 30 1 NOT REMARKABLE: 54 26 28 58 3 29 1 --EROSION --HEMORRHAGE --PARASITISM 1000000 1000010 0010000 COLON (CO) ........................ ................. NUMBER EXAMINED: 55 27 28 59 NOT REMARKABLE: 55 26 28 57 3 30 3 30 1 1 --PARASITISM 0102 000 RECTUM (RE) ....................... ................. NUMBER EXAMINED: 55 27 27 59 NOT REMARKABLE: 50 26 27 53 3 30 3 30 1 1 --PARASITISM --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 5105000 0001000 SKIN (SK) ......................... ................. NUMBER EXAMINED: 55 35 33 60 NOT REMARKABLE: 51 23 27 52 3 32 3 26 1 1 --ACUTE INFLAMMATORY EXUDATE --CYST, EPIDERMAL INCLUSION --EDEMA --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --NECROSIS 0000010 2110000 0100000 111102 0 042 0000 0000010 0100000 Covance 6329-212 3M T-6316.1 PAGE: 14 T E D -- 596 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- ---------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** SKIN (SK) ......................... ................. NUMBER EXAMINED: 55 35 33 60 3 32 1 NOT REMARKABLE: 51 23 27 52 3 26 1 --ULCERATION --B-FIBROMA --B-HEMANGIOMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL --M-SARCOMA, UNDIFFERENTIATED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 022 002 0 1110000 0000010 1251000 0103000 0302 030 0100000 0100000 0102 000 MAMMARY, FEMALE (MF) .............. ................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 URINARY BLADDER (UB) .......................... . .... NUMBER EXAMINED: 55 50 50 60 NOT REMARKABLE: 51 37 39 56 --CALCULUS --DESQUAMATION, TRANSITIONAL EPITHELIUM --EDEMA --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE 0 10 8 1 0010 0010 0000 1222 1000 0222 1110 3 40 3 35 03 00 00 02 00 01 01 00 1 1 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 15 A F F E C T E D -- 597 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) ...... NUMBER EXAMINED: 55 50 50 60 NOT REMARKABLE: 51 37 39 56 3 40 3 35 1 1 --ULCERATION --B-PAPILLOMA, TRANSITIONAL CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000010 1000000 0001000 CERVIX (CV) ......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 UTERUS (UT) NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 VAGINA (VA) NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PROSTATE (PR) --EDEMA --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE NUMBER EXAMINED: 55 31 33 60 NOT REMARKABLE: 13 7 5 22 0111 2000 0001 9627 4214 28 15 24 26 3 31 39 00 00 00 04 06 0 11 1 1 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 16 A F F E C T E D -- 598 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ** FROM PREVIOUS PAGE ** PROSTATE (PR) ..................... NUMBER EXAMINED: 55 31 33 60 NOT REMARKABLE: 13 7 5 22 3 31 39 1 1 --MINERALIZATION, LUMINAL --NECROSIS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0124 02 0 0001000 1001000 SEMINAL VESICLES (SV) ............. NUMBER EXAMINED: 55 28 28 60 NOT REMARKABLE: 48 26 27 51 3 30 3 24 1 1 --EDEMA --FIBROSIS --HYPERPLASIA, EPITHELIAL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ONE EXAMINED --SECRETION, INCREASED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1001010 0001000 0001000 100102 0 0102 000 1002 010 1000000 411304 0 1001000 EPIDIDYMIDES (EP) ................. NUMBER EXAMINED: 56 28 28 60 NOT REMARKABLE: 53 26 25 58 3 30 3 28 1 1 --HYPOSPERMIA --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1112 02 0 002 0000 1100000 1000000 Covance 6329-212 3M T-6316.1 PAGE: 17 T E D -- 599 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- CAUSE OF DEATH (CD) ................................. NUMBER EXAMINED: 70 60 60 70 70 40 40 NOT REMARKABLE: 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE --ADRENAL CORTICAL CARCINOMA --AUDITORY SEBACEOUS GLAND CARCINOMA --CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --FIBROSARCOMA --FRACTURE, HARD PALATE --HEMANGIOMA --HEPATIC NECROSIS --HEPATOCELLULAR ADENOMA --HISTIOCYTIC SARCOMA --MALIGNANT FIBROUS HISTIOSARCOMA --MALIGNANT LYMPHOMA --NECROSIS, ANAL MUSCULATURE --NEPHROBLASTOMA --PITUITARY ADENOMA --PYELONEPHRITIS --TRAUMA --UNDETERMINED --UNDIFFERENTIATED SARCOMA --UROLITHIASIS 34 32 32 37 67 10 39 1000000 0100000 0010000 2102 000 1000000 0100000 0000010 0 0 0 02 1 1 02 1 1 0 0 0 0111000 0001000 1101010 0000010 0100000 23 15 18 21 0 21 0 1010000 0010000 74 5614 0 0100000 0000010 Covance 6329-212 3M T-6316.1 PAGE: 18 T E D -- 600 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 T E D -- LN, MANDIBULAR (MN) ............... NUMBER EXAMINED: 7 8 3 5 0 5 0 NOT REMARKABLE: 1 2 0 1 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 010102 0 453302 0 0101030 0001000 2000000 LN, MEDIASTINAL (ML) .............. NUMBER EXAMINED: 4 1 3 3 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1122 000 101102 0 102 002 0 1001000 CAVITY, ABDOM (PC) ................ NUMBER EXAMINED: 0 0 1 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --NECROSIS, FAT --M-CARCINOMA 0000010 0010000 CAVITY, ORAL (OC) ................. NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --BACTERIAL COLONIES --FRACTURE 0100000 0100000 601 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- ---------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAVITY, ORAL (OC) ................................... NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE --INFLAMMATION, ACUTE 0100000 0100000 HEMATO NEOPLASIA (HN) ............................... NUMBER EXAMINED: 55 28 28 60 NOT REMARKABLE: 53 26 27 56 3 30 3 29 1 1 --M-LYMPHOMA --M-MALIGNANT FIBROUS HISTIOCYTOMA --M-SARCOMA, HISTIOCYTIC 2102 010 0001000 0111000 MESENTERY (MY) ...................................... NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE 1000000 LN, ANT MES/PANC (AP) ............................... NUMBER EXAMINED: 1 2 1 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) --N-ISLET CELL CARCINOMA 0010000 0111000 0001000 1100000 0010000 Covance 6329-212 3M T-6316.1 PAGE: 20 T E D -- 602 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 LN, LUMBAR/ILIAC (LM) ............. NUMBER EXAMINED: 4 9 4 4 0 1 0 NOT REMARKABLE: 0 2 0 0 0 1 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3634 000 0010000 0122 000 1000000 LN, RENAL (RL) .................... NUMBER EXAMINED: 3 1 1 2 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 --DILATATION, SINUSOIDAL --FIBROSIS --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2101000 0001000 2 01 0000 1001000 MAMMARY, MALE (MM) ................ NUMBER EXAMINED: 17 9 6 13 0 6 0 NOT REMARKABLE: 0 0 0 2 0 0 0 --GALACTOCELE --HEMORRHAGE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 12 6 4 5 0 3 0 1000000 12 0001 0 1000000 2224 03 0 0001000 3100000 1001000 Covance 6329-212 3M T-6316.1 PAGE: 21 A F F E C T E D -- 603 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- LN, AXILLARY (AX) ............. ..................... NUMBER EXAMINED: 2 3 0 3 0 1 0 NOT REMARKABLE: 2 2 0 2 0 1 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 0001000 0100000 CALVARIUM/SKULL (CA) .......... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 T E D -- TAIL (TI) ..................... ..................... NUMBER EXAMINED: 2 4 1 3 0 3 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --NECROSIS --B-KERATOACANTHOMA 1211010 2300010 0001000 1000000 0001000 2301010 0000010 0100000 ANUS (AN) ..................... ..................... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --NECROSIS, MUSCULAR 0000010 604 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL N U M B E R - O F - ANI M A SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) ............ ................... NUMBER EXAMINED: 11 7 12 7 0 10 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE --PIGMENT 42 92 02 0 8 7 12 7 0 7 0 342304 0 3 5 10 4 0 6 0 0001000 9 7 11 6 0 9 0 1011010 000002 0 LN, INGUINAL (IN) ............... ................... NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 1 0 0 0 0 JOINT, OTHER (JT) --DISLOCATION --EDEMA --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE, SYNOVIAL .... NUMBER EXAMINED: 1 1 1 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 1000000 1110000 0111000 0100000 1000000 0110000 0001000 1000000 Covance 6329-212 3M T-6316.1 PAGE: 23 A F F E C T E D -- 605 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) ............... ................. NUMBER EXAMINED: 1 1 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC ACTIVE --B-LIPOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0100000 1000000 0100000 0001000 AORTA (AO) ........................ ................. NUMBER EXAMINED: 3 0 1 0 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --MINERALIZATION 2 01 0000 PENIS (PE) ........................ ................. NUMBER EXAMINED: 1 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --EDEMA --HEMORRHAGE --INFLAMMATION, CHRONIC ACTIVE --PROLAPSE 1000000 1000000 1000000 1100000 PREPUTIAL GLAND (PG) .............. ................. NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC 0100000 Covance 6329-212 3M T-6316.1 PAGE: 24 T E D -- 606 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 --------------------------------- -=- -=- -=- -=- -=- -=- -=- AUDITORY SEB GL (AS) ............... ................ NUMBER EXAMINED: 0 1 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, ACUTE --M-CARCINOMA 0000010 0100010 AORTIC ARCH (AA) ................... ................ NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 1000000 LN, TRACHEOBRON (TB) ............... ................ NUMBER EXAMINED: 1 0 0 2 0 0 0 NOT REMARKABLE: 1 0 0 0 0 0 0 --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 0001000 URETER (UE) ........................ ................ NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION --INFLAMMATION, CHRONIC 0000010 0000010 PINNA (PN) ......................... ................ NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 Covance 6329-212 3M T-6316.1 PAGE: 25 T E D -- 607 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 ---------------- -=- -=- -=- -=- -=- -=- -=- LN, POPLITEAL (PP) .................................. NUMBER EXAMINED: 1 2 1 0 0 0 0 NOT REMARKABLE: 0 1 0 0 0 0 0 --DILATATION, SINUSOIDAL --HYPERPLASIA, LYMPHOCYTIC --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0010000 0110000 1000000 CAVITY, THORACIC (TA) ............................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 SUBCUTANEOUS TIS (SQ) ............................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 TONGUE (TO) ......................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 LN, OTHER (LN) ...................................... NUMBER EXAMINED: 0 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL --INFILTRATE, MACROPHAGE, PIGMENTED 0001000 0001000 Covance 6329-212 3M T-6316.1 PAGE: 26 T E D -- 608 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: --MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 -------------------------------------- -=- -=- -=- -=- -=- -=- -=- DIAPHRAGM (DP) ................ ..................... NUMBER EXAMINED: 1 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --B-FIBROMA 1000000 BILE DUCT (BD) ................ ..................... NUMBER EXAMINED: 0 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATED 0100000 Covance 6329-212 3M T-6316.1 PAGE: 27 T E D -- 609 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- --------------------------- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** EYE (EY) ................................. .......... NUMBER EXAMINED: 65 42 40 65 5 28 0 NOT REMARKABLE: 63 41 40 59 5 26 0 --HEMORRHAGE --HYPERPLASIA, CORNEAL EPITHELIUM --INFLAMMATION, ACUTE --INFLAMMATION, ACUTE, CILIARY --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, CHRONIC-ACTIVE, CORNEAL --MINERALIZATION, CORNEAL --ONE EXAMINED --SYNECHIA, ANTERIOR --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 0001000 0001000 1001000 0001000 0001000 0000010 0100000 0001000 0000010 2 001 000 0002 02 0 BRAIN (BR) ............................... .......... NUMBER EXAMINED: 65 48 48 65 NOT REMARKABLE: 27 10 12 33 5 34 5 10 0 0 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --PIGMENT --VACUOLATION, WHITE MATTER --M-ASTROCYTOMA --I-PITUITARY CARCINOMA 37 37 36 31 25 27 26 19 4774 0010 0000 1000 0000 0 23 0 13 02 00 01 00 01 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 28 A F F E C T E D -- 610 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- LIVER (LI) .......................................... NUMBER EXAMINED: 65 55 55 65 NOT REMARKABLE: 35 18 29 17 5 40 0 16 0 0 --CYST --CYST, BILIARY --DEGENERATION, CYSTIC --FIBROSIS --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --MINERALIZATION --NECROSIS, HEPATOCELLULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --PIGMENT --PIGMENT, HEPATOCELLULAR --PIGMENT, KUPFFER CELL --STASIS, BILE --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR 1002 000 0100000 02 02 010 0310010 1000000 0011000 372702 0 02 2 1 0 0 0 1103010 424 013 0 4 3 6 13 0 1 0 1 0 0 14 5 0 0 9 16 10 19 0 8 0 0100000 010002 0 1000000 5446130 0001000 0101000 0000010 0001000 0002 000 3614 05 0 0001010 13 14 8 26 0 8 0 Covance 6329-212 3M T-6316.1 PAGE: 29 T E D -- 611 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- ---------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .......................................... NUMBER EXAMINED: 65 55 55 65 5 40 0 NOT REMARKABLE: 35 18 29 17 0 16 0 --B-ADENOMA, HEPATOCELLULAR --M-CARCINOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0136030 0001000 0000010 LIVER, OIL RED O (LI1) .............................. NUMBER EXAMINED: 5 0 0 5 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --POSITIVE OIL RED O STAINING 5005000 KIDNEY (KD) ......................................... NUMBER EXAMINED: 65 55 55 65 NOT REMARKABLE: 15 7 15 23 5 40 44 0 0 --BACTERIAL COLONIES --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --B-LIPOMA 0000 14 20 14 18 3 0 04 5230 0101 0100 0000 2 001 37 34 28 29 9752 10 6 3 6 2213 0000 01 0 12 00 03 00 01 01 00 0 27 14 06 03 01 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 30 T E D -- 612 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 -- -=- -=- -=- -=- -=- -=- -=- SPLEEN (SP) ................................. ....... NUMBER EXAMINED: 65 42 40 65 NOT REMARKABLE: 57 37 33 58 5 28 5 21 0 0 --FIBROSIS, CAPSULAR --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED --B-HEMANGIOMA 0100000 5226060 3151010 0100000 PANCREAS (PA) ............................... ....... NUMBER EXAMINED: 65 55 55 65 NOT REMARKABLE: 56 48 48 59 5 40 5 35 0 0 --ATROPHY, ACINAR --HEMORRHAGE --HYPERPLASIA, ACINAR --HYPERPLASIA, ISLET CELLS --PIGMENT --B-ADENOMA, ISLET CELL --I-ENDOMETRIAL STROMAL SARCOMA 544504 0 1100000 1000000 1000000 2 021000 1411010 0100000 ADRENAL, CORTEX (AC) ........................ ....... NUMBER EXAMINED: 65 46 44 65 NOT REMARKABLE: 15 8 7 30 5 30 53 0 0 --ANGIECTASIS --ATROPHY, CORTICAL --CYST --DEGENERATION, CYSTIC, CORTICAL --FIBROSIS --HEMATOPOIESIS, EXTRAMEDULLARY 34 32 24 18 0001 0100 17 17 19 14 0002 0000 0 16 01 00 0 14 00 01 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 31 T E D -- 613 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................................ NUMBER EXAMINED: 65 46 44 65 NOT REMARKABLE: 15 8 7 30 5 30 53 0 0 --HEMORRHAGE --HYPERPLASIA, CORTICAL --HYPERTROPHY, CORTICAL CELL --MINERALIZATION --NECROSIS, CORTICAL --ONE EXAMINED --PIGMENT --THROMBUS --VACUOLATION --B-ADENOMA --M-CARCINOMA 48 37 34 26 0002 2 02 0 1101 0000 1000 7442 6624 4236 2 032 0000 0 20 00 00 02 01 00 02 04 01 01 01 0 0 0 0 0 0 0 0 0 0 0 ADRENAL, MEDULLA (MA) ............................... NUMBER EXAMINED: 65 45 44 65 NOT REMARKABLE: 64 42 43 61 5 30 5 28 0 0 --ONE EXAMINED --VACUOLATION --B-PHEOCHROMOCYTOMA --M-MALIGNANT PHEOCHROMOCYTOMA 1001010 0001010 0212 010 0100000 LN, MESENTERIC (MS) ................................. NUMBER EXAMINED: 64 41 43 64 NOT REMARKABLE: 57 39 35 60 5 28 5 25 0 0 --DILATATION, SINUSOIDAL --EDEMA 0012 000 1010000 Covance 6329-212 3M T-6316.1 PAGE: 32 T E D -- 614 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 33 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- -- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, MESENTERIC (MS) .................. .............. NUMBER EXAMINED: 64 41 43 64 5 28 0 NOT REMARKABLE: 57 39 35 60 5 25 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 2 033 01 0 527102 0 OVARY (OV) ........................... .............. NUMBER EXAMINED: 65 47 44 65 NOT REMARKABLE: 52 30 33 37 5 32 5 22 0 0 --CYST --HEMORRHAGE --MINERALIZATION --ONE EXAMINED --PIGMENTED --THROMBUS --B-GRANULOSA/THECA CELL TUMOR 9 13 11 18 1306 22 03 1 1 02 0003 0002 0001 0 10 00 00 01 00 00 00 0 0 0 0 0 0 0 TESTIS (TE) .......................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 T E D -- BONE, FEMUR (FE) ................. .................. NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 55 37 35 60 0 27 0 27 0 0 615 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- MARROW, FEMUR (FM) ............ ..................... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 54 37 35 60 0 27 0 27 0 0 --HEMORRHAGE 1000000 MARROW, STERNUM (SE) .......... ..................... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 55 37 35 60 0 28 0 28 0 0 BONE, STERNUM (SB) .................................. NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 54 37 35 57 --HYPERPLASIA, CARTILAGE 1003 SPINAL CORD (SC) .................................... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 55 37 34 60 --HEMORRHAGE 0010 THYMUS (TH) ......................................... NUMBER EXAMINED: 55 36 35 59 NOT REMARKABLE: 39 27 24 34 --CYST --DEPLETION, LYMPHOCYTIC --FIBROSIS --HEMORRHAGE --M-THYMIC CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 12 7 10 21 1010 0001 32 00 0002 1001 0 28 0 28 00 0 28 0 27 01 0 25 0 19 05 00 00 01 00 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 34 T E D -- 616 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 ------------------------------- -=- -=- -=- -=- -=- -=- -=- LUNG (LU) ............................ .............. NUMBER EXAMINED: 55 50 50 60 NOT REMARKABLE: 41 35 36 38 0 40 0 19 0 0 --EDEMA, ALVEOLAR --FIBROSIS, PLEURAL/SUBPLEURAL --FOREIGN BODY --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --INFLAMMATION, GRANULOMATOUS --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION --MINERALIZATION, VASCULAR --NECROSIS --THROMBUS --N-ADRENAL CORTICAL CARCINOMA --N-MALIGNANT PHEOCHROMOCYTOMA --N-MAMMARY CARCINOMA 0100010 1000000 0010000 012104 0 02 00010 2 002 050 0110000 0010000 8 2 5 12 0 9 0 0011000 02 0104 0 6867080 0000010 0001000 0000010 0100000 0001010 HEART (HT) ........................... .............. NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 46 32 30 58 0 28 0 28 0 0 --DEGENERATION/NECROSIS --FIBROSIS --MINERALIZATION 9542 000 5132 000 0011000 Covance 6329-212 3M T-6316.1 PAGE: 35 T E D -- 617 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 -------------------------------------- -=- -=- -=- -=- -=- -=- -=- MUSCLE, SKELETAL (SM) ......... ..................... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 54 37 34 60 0 28 0 28 0 0 --DEGENERATION/NECROSIS 1010000 NERVE, SCIATIC (SN) ........... ..................... NUMBER EXAMINED: 54 37 35 60 NOT REMARKABLE: 54 37 35 60 0 28 0 28 0 0 TRACHEA (TR) ................... .................... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 55 37 35 60 0 28 0 28 0 0 ESOPHAGUS (ES) .................. ................... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 55 37 35 60 0 28 0 28 0 0 THYROID (TY) .............................. ......... NUMBER EXAMINED: 55 50 50 60 NOT REMARKABLE: 33 25 34 31 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, CHRONIC --ONE EXAMINED --B-ADENOMA, C-CELL 22 13 11 26 10 00 00 00 8 13 03 7 13 12 12 00 00 01 8 11 0 40 0 25 01 09 01 01 01 01 00 08 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 36 T E D -- 618 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- -------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** THYROID (TY) .................. ..................... NUMBER EXAMINED: 55 50 50 60 0 40 0 NOT REMARKABLE: 33 25 34 31 0 25 0 --B-ADENOMA, FOLLICULAR CELL --M-CARCINOMA, C-CELL 1024 010 1000000 PARATHYROID (PT) .............. ..................... NUMBER EXAMINED: 52 34 34 58 NOT REMARKABLE: 51 34 34 58 0 27 0 27 0 0 --B-ADENOMA 1000000 PITUITARY (PI) ................ ..................... NUMBER EXAMINED: 55 48 49 59 NOT REMARKABLE: 7 1 1 12 0 38 02 0 0 --CYST --HEMORRHAGE --HYPERPLASIA --NECROSIS --PIGMENT --B-ADENOMA --M-CARCINOMA 2456 33 37 38 30 0000 0100 5887 48 46 48 45 0000 01 0 25 01 00 04 0 34 01 0 0 0 0 0 0 0 HARDERIAN GLAND (HG) .......... ..................... NUMBER EXAMINED: 55 37 35 59 NOT REMARKABLE: 44 31 27 51 0 28 0 26 0 0 --HEMORRHAGE --INFLAMMATION, CHRONIC --ONE EXAMINED 1000000 10 6 8 8 0 2 0 1000000 Covance 6329-212 3M T-6316.1 PAGE: 37 T E D -- 619 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO !ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 38 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 ---------------------- -=- -=- -=- -=- -=- -=- -=- SALIV GL, MANDIB (SG) ......................... ..... NUMBER EXAMINED: 55 37 35 59 NOT REMARKABLE: 55 37 35 59 0 27 0 27 0 0 STOMACH, GL (ST) .............................. ..... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 51 36 32 52 --CYST --EDEMA --EROSION --MINERALIZATION --INFLAMMATION, ACUTE 0000 3013 1125 0011 0010 STOMACH, NONGL (SU) ........................... ..... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 47 32 32 56 --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR MUCOSA --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION 7534 2 3 02 1000 1 0 02 1000 24 04 3000 2 002 DUODENUM (DU) ................................. ..... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 55 37 35 60 0 33 0 32 01 00 00 00 00 0 28 0 23 04 02 00 00 00 02 00 01 0 28 0 28 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 620 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 JEJUNUM (JE) ......................... ...... NUMBER EXAMINED: 54 38 33 59 NOT REMARKABLE: 53 36 33 59 --CYST, KERATIN --DIVERTICULUM --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) --B-ANGIOMA 0000 0100 0100 1000 ILEUM (IL) ........................... ...... NUMBER EXAMINED: 52 37 34 60 NOT REMARKABLE: 52 36 34 60 --HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE) 0100 CECUM (CE) ........................... ...... NUMBER EXAMINED: 54 37 35 60 NOT REMARKABLE: 52 37 35 60 --HEMORRHAGE --PARASITISM 1000 1000 COLON (CO) ........................... ...... NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 54 37 35 59 --EDEMA --PARASITISM 0001 1000 0 26 0 25 01 00 00 00 0 27 0 27 00 0 28 0 28 00 00 0 28 0 28 00 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 39 T E D -- 621 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 ---------------------------------- -=- -=- -=- -=- -=- -=- -=- RECTUM (RE) ....................... ................. NUMBER EXAMINED: 55 37 35 60 NOT REMARKABLE: 53 35 32 58 0 27 0 27 0 0 --PARASITISM 22 32 000 SKIN (SK) ......................... ................. NUMBER EXAMINED: 54 39 36 60 NOT REMARKABLE: 47 34 28 53 0 28 0 22 0 0 --ACUTE INFLAMMATORY EXUDATE --ATROPHY, ADNEXAL --CYST, EPIDERMAL INCLUSION --EDEMA --FAT NECROSIS --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --B-FIBROMA --B-KERATOACANTHOMA --B-LIPOMA --B-PAPILLOMA, SQUAMOUS CELL --M-FIBROSARCOMA --M-SEBACEOUS GLAND CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0100000 0001010 1000000 0010010 1000000 0001000 1112 000 433302 0 0011000 1002 02 0 4345050 0010000 0010000 1000000 0000010 0110000 0100000 0000010 Covance 6329-212 3M T-6316.1 PAGE: 40 T E D -- 622 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 MAMMARY, FEMALE (MF) .............. ................. NUMBER EXAMINED: 54 50 50 60 NOT REMARKABLE: 9 7 10 23 --EDEMA --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --M-CARCINOMA --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0000 33 32 34 28 5478 1 02 2 0143 0010 0010 6886 2242 3363 6463 23 21 16 13 9 9 15 11 1000 0000 URINARY BLADDER (UB) .............. ................. NUMBER EXAMINED: 55 50 49 60 NOT REMARKABLE: 53 50 49 58 --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --B-LEIOMYOMA --M-CARCINOMA, TRANSITIONAL CELL 0000 0002 1000 1000 0 40 05 01 0 27 03 00 00 00 01 04 00 01 04 0 12 06 00 01 0 40 0 38 01 02 00 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 41 T E D -- 623 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- CERVIX (CV) ......................... ............... NUMBER EXAMINED: 55 39 35 60 NOT REMARKABLE: 52 35 33 58 0 29 0 24 0 0 --CYST --DILATATION --HEMORRHAGE --HYPERTROPHY, SMOOTH MUSCLE --INFLAMMATION, ACUTE --NECROSIS --PIGMENT --B-LEIOMYOMA --B-POLYP, ENDOMETRIAL STROMAL --M-LEIOMYOSARCOMA --M-SARCOMA, ENDOMETRIAL STROMAL 1000000 1101010 0100010 0110000 0000010 0100000 1000000 0001010 12 01 03 0 0000010 0110000 UTERUS (UT) ......................... ............... NUMBER EXAMINED: 55 41 39 60 NOT REMARKABLE: 37 27 28 39 0 32 0 22 0 0 --CYST, KERATIN --DILATATION --HEMORRHAGE --HYPERPLASIA, CYSTIC, ENDOMETRIAL --HYPERPLASIA, ENDOMETRIAL --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --NECROSIS --PIGMENT --B-LEIOMYOMA 0001000 15 11 8 12 0 7 0 0012 01 0 331704 0 1001000 0011010 2000000 0000010 1001000 1000000 Covance 6329-212 3M T-6316.1 PAGE: 42 T E D -- 624 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 43 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: ------------- FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 ** FROM PREVIOUS PAGE ** UTERUS (UT) ............. .................. NUMBER EXAMINED: 55 41 39 60 NOT REMARKABLE: 37 27 28 39 0 32 0 22 0 0 --B-POLYP, ENDOMETRIAL, STROMAL --M-CARCINOMA --M-HEMANGIOSARCOMA --M-LEIOMYOSARCOMA 2223010 0010000 1000000 0001000 VAGINA (VA) ............. .................. NUMBER EXAMINED: 55 37 36 58 NOT REMARKABLE: 54 35 34 58 0 28 0 27 0 0 --HEMORRHAGE --B-LEIOMYOMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL --I-ENDOMETRIAL STROMAL SARCOMA --I-LEIOMYOSARCOMA 0100000 1000000 0100000 0010000 0010000 0000010 PROSTATE (PR) ........... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 T E D -- SEMINAL VESICLES (SV) ............... ............... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 625 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO !ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 44 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL N U M B E R - O F - ANI M A L S - A F F E SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- EPIDIDYMIDES (EP) .................. ................ NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 T E D -- CAUSE OF DEATH (CD) ................ ................ NUMBER EXAMINED: 70 60 60 70 70 40 40 NOT REMARKABLE: 0 0 0 0 0 0 0 --SCHEDULED SACRIFICE --ASTROCYTOMA --C-CELL CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --ENDOMETRIAL STROMAL POLYP --ENDOMETRIAL STROMAL SARCOMA --FIBROMA --FIBROSARCOMA --FRACTURE, CALVARIUM --HEMANGIOSARCOMA --HEPATOCELLULAR ADENOMA --KERATIN CYST --KERATOACANTHOMA --LIPOMA --LIPOSARCOMA --MALIGNANT FIBROUS HISTIOSARCOMA --MALIGNANT LYMPHOMA --MAMMARY CARCINOMA --MAMMARY FIBROADENOMA --PITUITARY ADENOMA --PITUITARY CARCINOMA 33 23 25 39 70 12 40 1000000 1000000 0000010 000002 0 0110000 0010000 0110000 0001000 1000000 0010000 0000010 0010000 1000000 0010000 0000010 1000000 036302 0 54 01 01 0 27 28 22 17 0 18 0 0001010 626 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- -- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAUSE OF DEATH (CD) .................. .............. NUMBER EXAMINED: 70 60 60 70 70 40 40 NOT REMARKABLE: 0 0 0 0 0 0 0 --RENAL MINERALIZATION --SQUAMOUS CELL CARCINOMA --THYMIC CARCINOMA --UNDETERMINED --UTERINE HEMORRHAGE 0001000 0010000 0001000 0005010 0001000 LN, MANDIBULAR (MN) .................. .............. NUMBER EXAMINED: 9 6 9 9 0 10 0 NOT REMARKABLE: 4 2 3 4 0 3 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 001002 0 42 54 05 0 121102 0 0100000 LN, MEDIASTINAL (ML) ................. .............. NUMBER EXAMINED: 3 2 5 7 1 4 0 NOT REMARKABLE: 0 0 1 0 0 1 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 2214130 214602 0 CAVITY, ABDOM (PC) ................... .............. NUMBER EXAMINED: 0 4 1 3 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --FIBROSIS --HEMORRHAGE 0001000 0101000 Covance 6329-212 3M T-6316.1 PAGE: 45 T E D -- 627 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CAVITY, ABDOM (PC) ............... .................. NUMBER EXAMINED: 0 4 1 3 0 2 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFLAMMATION, CHRONIC --MINERALIZATION --NECROSIS, FAT --PIGMENT --THROMBUS --I-ENDOMETRIAL STROMAL SARCOMA 0001000 0101000 031302 0 0001000 0001000 0100000 CAVITY, ORAL (OC) ................ .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 HEMATO NEOPLASIA (HN) ............................... NUMBER EXAMINED: 55 37 35 59 NOT REMARKABLE: 54 37 35 58 --M-LYMPHOMA --M-MALIGNANT FIBROUS HISTIOCYTOMA 1001 0000 MESENTERY (MY) ....................................... NUMBER EXAMINED: 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 28 0 27 00 01 00 00 0 0 0 0 0 0 LN, ANT MES/PANC (AP) ............................... NUMBER EXAMINED: 0 1 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE 0100000 Covance 6329-212 3M T-6316.1 PAGE: 46 A F F E C T E D -- 628 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 47 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION NUMBER: 70 60 60 70 70 40 40 -- ------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, ANT MES/PANC (AP) ................ .............. NUMBER EXAMINED: 0 1 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --INFILTRATE, MACROPHAGE, PIGMENTED 0001000 LN, LUMBAR/ILIAC (LM) ................ .............. NUMBER EXAMINED: 5 6 5 4 0 6 0 NOT REMARKABLE: 3 5 3 4 0 2 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --N-ENDOMETRIAL STROMAL SARCOMA 2 00001 0 111002 0 100002 0 0010000 LN, RENAL (RL) ....................... .............. NUMBER EXAMINED: 1 2 0 2 0 2 0 NOT REMARKABLE: 0 1 0 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 1102 010 0000010 0001000 MAMMARY, MALE (MM) ................... .............. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 T E D -- LN, AXILLARY (AX) .................... .............. NUMBER EXAMINED: 15 17 18 12 0 6 0 NOT REMARKABLE: 9 12 11 4 0 4 0 --DILATATION, SINUSOIDAL 0111000 629 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 48 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: FEMALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 -- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LN, AXILLARY (AX) ................................... NUMBER EXAMINED: 15 17 18 12 0 6 0 NOT REMARKABLE: 9 12 11 4 0 4 0 --EDEMA --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0010000 4435000 2 022 010 1113000 0000010 CALVARIUM/SKULL (CA) ................................ NUMBER EXAMINED: 0 1 0 1 0 0 0 NOT REMARKABLE: 0 1 0 0 0 0 0 --FRACTURE --HEMORRHAGE 0001000 0001000 TAIL (TI) ........................................... NUMBER EXAMINED: 0 0 1 3 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EROSION --INFLAMMATION, ACUTE 0003000 0010010 0013010 ANUS (AN) ........................................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 T E D -- 630 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- NUMBER: 70 60 60 70 70 40 40 ---------------- -=- -=- -=- -=- -=- -=- -=- FOOT/FOOTPAD(S) (FP) ................................ NUMBER EXAMINED: 7 3 3 1 0 4 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --CYST, EPIDERMAL INCLUSION --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE 0100000 2100010 72 01 03 0 2100000 5230030 0100000 732 004 0 0011000 LN, INGUINAL (IN) ................................... NUMBER EXAMINED: 4 4 4 2 0 0 0 NOT REMARKABLE: 1 3 3 0 0 0 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --N-MAMMARY CARCINOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0001000 1110000 1001000 0001000 1000000 JOINT, OTHER (JT) ................................... NUMBER EXAMINED: 3 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --EXOSTOSES --FIBROSIS --INFLAMMATION, CHRONIC 3010000 1010000 2000000 Covance 6329-212 3M T-6316.1 PAGE: 49 T E D -- 631 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: -FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- ADIPOSE TISSUE (AT) .............. .................. NUMBER EXAMINED: 1 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --FIBROSIS --HEMORRHAGE --I-TRANSITIONAL CELL CARCINOMA 0100000 0100000 1000000 AORTA (AO) ....................... .................. NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --MINERALIZATION 0000010 PENIS (PE) ....................... .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PREPUTIAL GLAND (PG) ............. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 AUDITORY SEB GL (AS) ............. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 AORTIC ARCH (AA) ................. .................. NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 50 T E D -- 632 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M BE R -O F -A N IMA SEX: FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN AND FINDING DESCRIPTION -- -- NUMBER: 70 60 60 70 70 40 40 -=- -=- -=- -=- -=- -=- -=- LN, TRACHEOBRON (TB) .......... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 URETER (UE) ................... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 PINNA (PN) .................... ..................... NUMBER EXAMINED: 0 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --HEMORRHAGE 0001000 LN, POPLITEAL (PP) ............ ..................... NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --DILATATION, SINUSOIDAL 0010000 CAVITY, THORACIC (TA) ......... ..................... NUMBER EXAMINED: 0 0 1 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 --M-LIPOSARCOMA 0010000 SUBCUTANEOUS TIS (SQ) ......... ..................... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 51 T E D -- 633 TABLE 45 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - ANI M A SEX: -FEMALE GROUP: -1- -2- -3- -4- -5- -6- -7- NUMBER: 70 60 60 70 70 40 40 TONGUE (TO) .................. --CYST LN, OTHER (LN) ............... NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0000010 NUMBER EXAMINED: 0 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 1 0 DIAPHRAGM (DP) ............... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 BILE DUCT (BD) ............... NUMBER EXAMINED: 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 52 T E D -- 634 TABLE 46 Incidence of Severity of Selected Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ----------------------------------- ----------------------------------- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ....................... .................. NUMBER EXAMINED: 5 5 5 5 --CYST -> 5 5 5 5 TL> 5 5 5 5 MN> oooooo oooooo oooooo oooooo oooooo oooooo oooooo oooooo --CYST, BILIARY -> 5 5 5 5 TL> 5 5 5 5 MN> --DEGENERATION, CYSTIC -> 5 5 5 5 TL> 5 5 5 5 MN> --FIBROSIS -> 5 5 5 5 TL> 5 5 5 5 MN> --FIBROSIS, CAPSULAR -> 5 5 5 5 TL> 5 5 5 5 MN> --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 5 5 5 5 TL> 5 5 5 5 MN> 635 TABLE 46 Incidence of Severity of Selected Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ** FROM PREVIOUS PAGE ** LIVER (LI) ....................... .... NUMBER EXAMINED: 5 5 5 5 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 5 5 5 5 TL> 5 5 5 5 MN> ooo ooo ooo ooo ooo ooo ooo ooo --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 5 5 5 5 TL> 5 5 5 5 MN> --HEMATOPOIESIS, EXTRAMEDULLARY -> 5 5 5 5 TL> 5 5 5 5 MN> --HEMORRHAGE -> 5 2 1> 0 3 3> 0 0 TL> 5 5 MN> 0.6 54 00 01 55 0.6 o o oo oo o o oo oo --HYPERPLASIA, BILE DUCT -> 5 5 5 5 TL> 5 5 5 5 MN> 636 TABLE 46 Incidence of Severity of Selected Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ** FROM PREVIOUS PAGE ** LIVER (LI) .................................... ..... --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR NUMBER EXAMINED: -> 1> 3> 4> TL> MN> 5 5 0 0 0 5 555 04 0 010 5 04 001 555 0.2 3.2 ooo ooo ooo ooo o ooo ro o o o o oo o oo --INFLAMMATION, ACUTE -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 3 5 5 5 1> 2 0 0 0 TL> 5 5 5 5 MN> 0.4 --INFLAMMATION, CHRONIC-ACTIVE -> 5 5 5 5 TL> 5 5 5 5 MN> --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 5 5 5 5 TL> 5 5 5 5 MN> 637 TABLE 46 Incidence of Severity of Selected Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ------------------------------------- --------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ......................... ................ NUMBER EXAMINED: 5 5 5 5 --MINERALIZATION -> 5 5 5 5 TL> 5 5 5 5 MN> o oooo o oooo o ooooo o ooooo o oooo o oooo o ooooo o ooooo --NECROSIS, HEPATOCELLULAR -> 5 2 1> 0 1 3> 0 2 TL> 5 5 MN> 1.4 54 00 01 55 0.6 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 5 5 5 5 TL> 5 5 5 5 MN> --PIGMENT -> 5 5 5 5 TL> 5 5 5 5 MN> --PIGMENT, HEPATOCELLULAR -> 5 5 5 5 TL> 5 5 5 5 MN> --PIGMENT, KUPFFER CELL -> 5 5 5 5 TL> 5 5 5 5 MN> 638 TABLE 46 Incidence of Severity of Selected Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ** FROM PREVIOUS PAGE ** LIVER (LI) ..................... .................... NUMBER EXAMINED: 5 5 5 5 --STASIS, BILE -> 5 5 5 5 TL> 5 5 5 5 MN> 0 .0 0 .0 ooo ooo ooo ooo --TELANGIECTASIS -> 5 5 5 5 TL> 5 5 5 5 MN> 0 .0 0 .0 --THROMBUS -> 5 5 5 5 TL> 5 5 5 5 MN> 0 .0 0 .0 --VACUOLATION, HEPATOCELLULAR -> 5 1> 0 TL> 5 MN> 0 .0 4 1 5 0.2 o o 55 00 55 0 .0 --B-ADENOMA, HEPATOCELLULAR -> 5 5 5 5 TL> 5 5 5 5 --B-CHOLANGIOMA -> 5 5 5 5 TL> 5 5 5 5 --M-CARCINOMA, HEPATOCELLULAR -> 5 5 5 5 TL> 5 5 5 5 639 TABLE 46 Incidence of Severity of Selected Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 ** FROM PREVIOUS PAGE ** -=- -=- -=- -=- LIVER (LI) ........................ ................. NUMBER EXAMINED: 5 5 5 5 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) -> 5 5 5 5 ** END OF LIST ** TL> 5 5 5 5 640 TABLE 47 Incidence of Severity of Selected Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S SEX: ---MALE--- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ----------------------------------- ----------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ....................... .................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --CYST -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --CYST, BILIARY -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FIBROSIS -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FIBROSIS, CAPSULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Covance 6329-212 3M T-6316.1 PAGE: 1 641 TABLE 47 Incidence of Severity of Selected Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S SEX: ---MALE--- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ** FROM PREVIOUS PAGE ** LIVER (LI) ....................... .... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --HEMATOPOIESIS, EXTRAMEDULLARY -> 4 5 5 5 5 5 5 5 1> 1 0 0 0 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.2 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --HEMORRHAGE -> 4 5 5 5 4 5 5 5 1> 1 0 0 0 0 0 0 0 3> 0 0 0 0 1 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.2 0.0 0.0 0.0 0.6 0.0 0.0 0.0 --HYPERPLASIA, BILE DUCT -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Covance 6329-212 3M T-6316.1 PAGE: 2 642 TABLE 47 Incidence of Severity of Selected Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S SEX: ---MALE--- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------ -- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .................................... ..... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 5 4 2 0 5 5 5 3 1> 0 1 1 0 0 0 0 0 2> 0 0 1 4 0 0 0 2 3> 0 0 1 1 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.2 1.2 2.2 0.0 0.0 0.0 0.8 --INFLAMMATION, ACUTE -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 4 4 2 5 4 1 3 3 1> 0 1 3 0 1 4 2 2 2> 1 0 0 0 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.4 0.2 0.6 0.0 0.2 0.8 0.4 0.4 --INFLAMMATION, CHRONIC-ACTIVE -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Covance 6329-212 3M T-6316.1 PAGE: 3 643 TABLE 47 Incidence of Severity of Selected Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S SEX: ---MALE--- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------- --------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ......................... ................ NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --MINERALIZATION -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --NECROSIS, HEPATOCELLULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT, HEPATOCELLULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT, KUPFFER CELL -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Covance 6329-212 3M T-6316.1 PAGE: 4 644 TABLE 47 Incidence of Severity of Selected Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S SEX: ---MALE--- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ** FROM PREVIOUS PAGE ** LIVER (LI) ...................... ................... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --STASIS, BILE -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --THROMBUS -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --VACUOLATION, HEPATOCELLULAR -> 5 3 3 4 5 5 5 5 1> 0 1 1 1 0 0 0 0 2> 0 1 0 0 0 0 0 0 3> 0 0 1 0 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.6 0.8 0.2 0.0 0.0 0.0 0.0 --B-ADENOMA, HEPATOCELLULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 --B-CHOLANGIOMA -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 Covance 6329-212 3M T-6316.1 PAGE: 5 645 TABLE 47 Incidence of Severity of Selected Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -- MALE --- --- --- --FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------ ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 5 5 5 5 5 5 5 5 --M-CARCINOMA, HEPATOCELLULAR -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 5 5 5 5 5 5 5 5 TL> 5 5 5 5 5 5 5 5 646 TABLE 48 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 -- ----------------------------------- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ....................... .................. NUMBER EXAMINED: 5 10 5 10 --CYST -> 5 10 5 10 TL> 5 10 5 10 MN> oo oo oo oo oo oo oo oo --CYST, BILIARY -> 5 10 TL> 5 10 MN> 5 10 5 10 --DEGENERATION, CYSTIC -> 5 10 1> 0 0 TL> 5 10 MN> 59 01 5 10 0.1 ooo ooo oooo oooo oooo oooo oooo oooo --FIBROSIS -> 5 10 TL> 5 10 MN> 5 10 5 10 --FIBROSIS, CAPSULAR -> 5 10 TL> 5 10 MN> 5 10 5 10 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 5 10 TL> 5 10 MN> 5 10 5 10 647 TABLE 48 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ** FROM PREVIOUS PAGE ** LIVER (LI) ....................... .... NUMBER EXAMINED: 5 10 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 5 10 4> 0 0 TL> 5 10 MN> 5 10 59 01 5 10 0.4 ooo ooo oooo oooo oooo oooo oooo oooo --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 5 10 TL> 5 10 MN> 5 10 5 10 --HEMATOPOIESIS, EXTRAMEDULLARY -> 5 10 TL> 5 10 MN> 5 10 5 10 --HEMORRHAGE -> 5 10 TL> 5 10 MN> 5 10 5 10 --HYPERPLASIA, BILE DUCT o o o o o o -> 5 10 1> 0 0 TL> 5 10 MN> 59 01 5 10 0.1 648 TABLE 48 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ------------------------------------------------ -- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .................................... ..... NUMBER EXAMINED: 5 10 5 10 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 5 0 5 3 2> 0 3 0 3 3> 0 2 0 4 4> 0 5 0 0 TL> 5 10 5 10 MN> 3.2 1.8 o o o o o o o o --INFLAMMATION, ACUTE -> 5 TL> 5 MN> 0.0 10 5 10 5 0.0 10 10 --INFLAMMATION, CHRONIC -> 2 9 4 9 1> 3 1 1 0 2> 0 0 0 1 TL> 5 10 5 10 MN> 0.6 0.1 0.2 0.2 --INFLAMMATION, CHRONIC-ACTIVE -> 5 10 TL> 5 10 MN> 5 10 5 10 oo oo oo oo oo oo oo oo --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 5 10 TL> 5 10 MN> 5 10 5 10 649 TABLE 48 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ------------------------------------- --------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ......................... ................ NUMBER EXAMINED: 5 10 5 10 --MINERALIZATION -> 5 10 5 10 TL> 5 10 5 10 MN> o oooo o oooo o ooooo o ooooo o oooo o oooo o ooooo o ooooo --NECROSIS, HEPATOCELLULAR -> 5 9 1> 0 1 3> 0 0 TL> 5 10 MN> 0.1 58 01 01 5 10 0.4 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 5 10 TL> 5 10 MN> 5 10 5 10 --PIGMENT -> 5 10 TL> 5 10 MN> 5 10 5 10 --PIGMENT, HEPATOCELLULAR -> 5 10 TL> 5 10 MN> 5 10 5 10 --PIGMENT, KUPFFER CELL -> 5 10 TL> 5 10 MN> 5 10 5 10 650 TABLE 48 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ** FROM PREVIOUS PAGE ** LIVER (LI) ..................... .................... --STASIS, BILE NUMBER EXAMINED: -> 1> 2> TL> MN> 5 10 59 01 00 5 10 0.1 5 10 58 01 01 5 10 ro o oo oo oo o o oo oo --TELANGIECTASIS -> 5 10 TL> 5 10 MN> 5 10 5 10 --THROMBUS -> 5 10 TL> 5 10 MN> 5 10 5 10 o o o o o o o o --VACUOLATION, HEPATOCELLULAR o o o o -> 5 1 1> 0 3 2> 0 3 3> 0 3 TL> 5 10 MN> 1.8 58 02 00 00 5 10 0.2 --B-ADENOMA, HEPATOCELLULAR -> 5 10 +> 0 0 TL> 5 10 59 01 5 10 651 TABLE 48 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,4;WEEKS=ALL DEATH=4;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 ------------------------------------ ---------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 5 10 5 10 --B-CHOLANGIOMA -> 5 10 5 10 TL> 5 10 5 10 --M-CARCINOMA, HEPATOCELLULAR -> 5 10 TL> 5 10 5 10 5 10 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 5 10 TL> 5 10 5 10 5 10 652 TABLE 49 Incidence of Severity of Selected Microscopic Observations Terminal and Recovery Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------ ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --CYST -> 13 22 22 17 10 13 13 15 19 12 3> 0 0 0 0 0 0 0 0 1 0 4> 1 0 0 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.2 0.0 --CYST, BILIARY -> 14 22 22 17 10 13 12 15 20 12 3> 0 0 0 0 0 0 1 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.2 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 11 17 10 5 9 13 11 15 19 11 1> 2 0 1 5 0 0 1 0 0 0 2> 0 1 2 4 1 0 1 0 1 1 3> 0 3 6 3 0 0 0 0 0 0 4> 1 1 3 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.4 0.7 1.6 1.3 0.2 0.0 0.2 0.0 0.1 0.2 --FIBROSIS -> 14 22 21 17 9 13 13 15 20 11 1> 0 0 1 0 1 0 0 0 0 0 2> 0 0 0 0 0 0 0 0 0 1 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 0.0 0.2 653 TABLE 49 Incidence of Severity of Selected Microscopic Observations Terminal and Recovery Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T -- N U M B E R - O F - ANI M A LS- A F F E ' SEX: MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ** FROM PREVIOUS PAGE ** LIVER (LI) ....................... --FIBROSIS, CAPSULAR .... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 -> 14 22 22 17 10 12 13 15 20 12 3> 0 0 0 0 0 1 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.2 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 13 21 21 17 10 13 13 14 20 12 2> 0 0 1 0 0 0 0 0 0 0 3> 1 0 0 0 0 0 0 1 0 0 4> 0 1 0 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.2 0.2 0.1 0.0 0.0 0.0 0.0 0.2 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 11 12 11 12 9 13 9 13 15 11 1> 0 3 2 0 0 0 0 0 0 0 2> 1 4 2 1 0 0 1 1 1 0 3> 2 3 4 2 1 0 3 0 2 1 4> 0 0 2 2 0 0 0 0 2 0 5> 0 0 1 0 0 0 0 1 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.6 0.9 1.4 0.9 0.3 0.0 0.8 0.5 0.8 0.3 654 TABLE 49 Incidence of Severity of Selected Microscopic Observations Terminal and Recovery Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ----------------------------------------------- ----------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ................................... ...... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 14 22 20 16 10 13 11 14 19 12 1> 0 0 2 0 0 0 1 1 1 0 2> 0 0 0 0 0 0 1 0 0 0 4> 0 0 0 1 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.1 0.2 0.0 0.0 0.2 0.1 0.1 0.0 --HEMATOPOIESIS, EXTRAMEDULLARY -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --HEMORRHAGE -> 14 20 21 16 8 12 12 14 20 11 1> 0 1 1 0 2 0 1 1 0 0 2> 0 1 0 1 0 0 0 0 0 0 3> 0 0 0 0 0 1 0 0 0 1 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.1 0.0 0.1 0.2 0.2 0.1 0.1 0.0 0.3 --HYPERPLASIA, BILE DUCT -> 10 15 16 13 9 12 12 12 13 11 1> 4 7 6 3 1 1 1 2 6 1 2> 0 0 0 1 0 0 0 1 1 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.3 0.3 0.3 0.3 0.1 0.1 0.1 0.3 0.4 0.1 655 TABLE 49 Incidence of Severity of Selected Microscopic Observations Terminal and Recovery Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------ ---------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .................................... ..... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 14 22 22 12 10 13 13 15 15 12 1> 0 0 0 1 0 0 0 0 1 0 2> 0 0 0 2 0 0 0 0 3 0 3> 0 0 0 1 0 0 0 0 1 0 4> 0 0 0 1 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.7 0.0 0.0 0.0 0.0 0.5 0.0 --INFLAMMATION, ACUTE -> 14 22 22 17 9 13 13 15 20 12 1> 0 0 0 0 1 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 10 9 10 9 7 12 11 13 12 11 1> 4 13 11 8 3 1 2 2 6 1 2> 0 0 1 0 0 0 0 0 2 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.3 0.6 0.6 0.5 0.3 0.1 0.2 0.1 0.5 0.1 --INFLAMMATION, CHRONIC-ACTIVE -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 656 TABLE 49 Incidence of Severity of Selected Microscopic Observations Terminal and Recovery Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------------------ -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .................................... ..... NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 14 20 22 16 10 13 12 15 20 10 2> 0 1 0 0 0 0 0 0 0 0 3> 0 0 0 0 0 0 0 0 0 2 4> 0 0 0 1 0 0 0 0 0 0 5> 0 1 0 0 0 0 1 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.3 0.0 0.2 0.0 0.0 0.4 0.0 0.0 0.5 --MINERALIZATION -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --NECROSIS, HEPATOCELLULAR -> 14 21 20 17 9 12 13 14 20 11 1> 0 0 1 0 0 0 0 1 0 0 2> 0 1 1 0 0 0 0 0 0 0 3> 0 0 0 0 0 0 0 0 0 1 4> 0 0 0 0 1 1 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.1 0.1 0.0 0.4 0.3 0.0 0.1 0.0 0.3 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 657 TABLE 49 Incidence of Severity of Selected Microscopic Observations Terminal and Recovery Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------ ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --PIGMENT -> 14 21 21 16 9 13 13 15 19 12 1> 0 0 0 0 1 0 0 0 0 0 2> 0 1 1 1 0 0 0 0 1 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.1 0.1 0.1 0.1 0.0 0.0 0.0 0.1 0.0 --PIGMENT, HEPATOCELLULAR -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT, KUPFFER CELL -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --STASIS, BILE -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 12 16 20 17 10 12 11 15 16 8 1> 1 2 1 0 0 0 1 0 2 3 2> 0 2 1 0 0 0 1 0 1 1 3> 1 2 0 0 0 1 0 0 1 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.3 0.5 0.1 0.0 0.0 0.2 0.2 0.0 0.4 0.4 658 TABLE 49 Incidence of Severity of Selected Microscopic Observations Terminal and Recovery Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ------------------------------------ ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --THROMBUS -> 14 21 22 17 10 13 13 15 19 12 1> 0 0 0 0 0 0 0 0 1 0 2> 0 1 0 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.0 0.1 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 --VACUOLATION, HEPATOCELLULAR -> 10 14 13 4 9 9 7 13 7 10 1> 1 3 6 3 1 1 4 1 7 1 2> 2 2 2 5 0 0 2 1 4 1 3> 0 2 1 3 0 3 0 0 1 0 4> 1 1 0 1 0 0 0 0 0 0 5> 0 0 0 1 0 0 0 0 1 0 TL> 14 22 22 17 10 13 13 15 20 12 MN> 0.6 0.8 0.6 1.8 0.1 0.8 0.6 0.2 1.2 0.3 --B-ADENOMA, HEPATOCELLULAR -> 13 20 21 14 10 13 12 14 16 10 +> 1 2 1 3 0 0 1 1 4 2 TL> 14 22 22 17 10 13 13 15 20 12 --B-CHOLANGIOMA -> 14 22 22 17 10 13 13 15 20 12 TL> 14 22 22 17 10 13 13 15 20 12 --M-CARCINOMA, HEPATOCELLULAR -> 14 22 22 17 10 13 13 15 19 12 +> 0 0 0 0 0 0 0 0 1 0 TL> 14 22 22 17 10 13 13 15 20 12 659 TABLE 49 Incidence of Severity of Selected Microscopic Observations Terminal and Recovery Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=T,U;FIND=ALL;SUBSET=T -- N U M BER - O F - ANI MALS- A F F E SEX: MALE-- --- FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 14 22 22 17 10 13 13 15 20 12 ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 14 22 22 17 10 13 13 15 20 12 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) -> 13 22 22 17 10 13 13 15 20 12 +> 1 0 0 0 0 0 0 0 0 0 TL> 14 22 22 17 10 13 13 15 20 12 ** END OF LIST ** 660 TABLE 50 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE SEX: -MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------ ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --CYST -> 36 28 27 31 30 36 37 35 28 28 1> 0 0 0 0 0 0 0 0 1 0 2> 0 0 0 2 0 1 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.1 0.0 0.1 0.0 0.0 0.0 0.0 --CYST, BILIARY -> 36 28 27 33 30 37 37 35 29 28 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --DEGENERATION, CYSTIC -> 33 25 25 27 24 37 37 35 29 28 1> 1 1 0 0 2 0 0 0 0 0 2> 0 1 1 4 3 0 0 0 0 0 3> 2 1 1 1 1 0 0 0 0 0 4> 0 0 0 1 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.2 0.2 0.2 0.5 0.4 0.0 0.0 0.0 0.0 0.0 --FIBROSIS -> 36 28 27 33 29 37 34 34 29 28 1> 0 0 0 0 0 0 2 1 0 0 3> 0 0 0 0 1 0 1 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.1 0.0 0.1 0.0 0.0 0.0 661 TABLE 50 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M B E R - O F - ANI M A LS- A F F E ' SEX: MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ** FROM PREVIOUS PAGE ** LIVER (LI) ....................... --FIBROSIS, CAPSULAR .... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 -> 36 27 27 33 30 37 37 35 29 28 1> 0 1 0 0 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL -> 36 28 27 33 30 37 37 35 28 28 3> 0 0 0 0 0 0 0 0 1 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 31 26 27 32 29 34 34 35 28 27 1> 2 0 0 0 0 2 0 0 0 0 2> 2 1 0 0 0 0 2 0 0 0 3> 1 0 0 1 1 1 1 0 0 0 5> 0 1 0 0 0 0 0 0 1 1 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.3 0.3 0.0 0.1 0.1 0.1 0.2 0.0 0.2 0.2 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 35 28 27 33 30 37 37 34 29 28 1> 1 0 0 0 0 0 0 0 0 0 2> 0 0 0 0 0 0 0 1 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 0.0 662 TABLE 50 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------------------ ---------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .................................... ..... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --HEMATOPOIESIS, EXTRAMEDULLARY -> 36 28 27 32 30 36 36 35 26 27 1> 0 0 0 1 0 1 1 0 2 1 2> 0 0 0 0 0 0 0 0 1 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 --HEMORRHAGE -> 34 27 25 33 25 35 36 32 29 26 1> 1 1 0 0 2 1 0 2 0 0 2> 1 0 1 0 0 1 0 1 0 0 3> 0 0 1 0 3 0 1 0 0 2 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.1 0.0 0.2 0.0 0.4 0.1 0.1 0.1 0.0 0.2 --HYPERPLASIA, BILE DUCT -> 31 26 25 26 26 34 35 32 24 28 1> 2 2 0 6 2 3 2 3 5 0 2> 3 0 2 1 1 0 0 0 0 0 3> 0 0 0 0 1 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.2 0.1 0.1 0.2 0.2 0.1 0.1 0.1 0.2 0.0 --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 36 28 27 31 28 37 37 35 29 28 2> 0 0 0 0 1 0 0 0 0 0 3> 0 0 0 2 1 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.2 0.2 0.0 0.0 0.0 0.0 0.0 663 TABLE 50 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------------------ -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .................................... ..... NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --INFLAMMATION, ACUTE -> 36 28 27 33 30 37 37 35 29 28 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 28 22 20 22 24 31 27 29 21 21 1> 8 6 7 11 6 5 8 6 8 7 2> 0 0 0 0 0 1 2 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.2 0.2 0.3 0.3 0.2 0.2 0.3 0.2 0.3 0.3 --INFLAMMATION, CHRONIC-ACTIVE -> 36 28 26 33 30 37 36 35 29 28 2> 0 0 0 0 0 0 1 0 0 0 3> 0 0 1 0 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.1 0.0 0.0 0.0 0.1 0.0 0.0 0.0 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 36 28 26 32 30 37 37 35 29 28 4> 0 0 0 1 0 0 0 0 0 0 5> 0 0 1 0 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.2 0.1 0.0 0.0 0.0 0.0 0.0 0.0 --MINERALIZATION -> 36 28 27 33 30 36 37 35 29 28 2> 0 0 0 0 0 1 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 0.0 664 TABLE 50 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------- -- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ......................... ................ NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --NECROSIS, HEPATOCELLULAR -> 35 26 25 32 27 33 33 32 25 26 1> 0 1 0 0 0 1 1 0 1 0 2> 0 0 1 1 0 1 1 3 1 0 3> 1 1 0 0 3 2 2 0 1 1 4> 0 0 0 0 0 0 0 0 1 1 5> 0 0 1 0 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.1 0.1 0.3 0.1 0.3 0.2 0.2 0.2 0.3 0.3 --NECROSIS, INDIVIDUAL HEPATOCYTE -> 36 28 27 33 30 37 37 35 28 28 1> 0 0 0 0 0 0 0 0 1 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --PIGMENT -> 36 28 27 33 30 37 36 35 29 28 2> 0 0 0 0 0 0 1 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 --PIGMENT, HEPATOCELLULAR -> 36 28 27 33 30 37 37 35 29 27 3> 0 0 0 0 0 0 0 0 0 1 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 665 TABLE 50 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE FEMALE--- GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------ ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --PIGMENT, KUPFFER CELL -> 36 28 27 33 30 37 37 35 28 28 1> 0 0 0 0 0 0 0 0 1 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --STASIS, BILE -> 36 28 27 33 29 37 37 35 29 28 3> 0 0 0 0 1 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.1 0.0 0.0 0.0 0.0 0.0 --TELANGIECTASIS -> 36 27 26 33 30 35 33 34 29 27 1> 0 0 0 0 0 1 2 0 0 1 2> 0 0 0 0 0 0 1 1 0 0 3> 0 1 1 0 0 1 1 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.1 0.1 0.0 0.0 0.1 0.2 0.1 0.0 0.0 --THROMBUS -> 36 28 27 33 30 37 37 35 29 27 3> 0 0 0 0 0 0 0 0 0 1 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.1 666 TABLE 50 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3/4 /6;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA L S -A F FE ' SEX: -MALE FEMALE GROUP: -1- -2- -3- -4- -6- -1- -2- -3- -4- -6- ORGAN/TISSUE EXAMINED NUMBER: 36 28 27 33 30 37 37 35 29 28 ------------------------------------ ---------------------------------- -=- -=- -=- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 36 28 27 33 30 37 37 35 29 28 --VACUOLATION, HEPATOCELLULAR -> 23 20 16 17 20 28 29 29 18 22 1> 5 3 1 5 3 2 2 3 4 2 2> 2 2 0 2 2 1 0 2 4 1 3> 4 2 6 5 3 2 4 0 3 1 4> 0 0 3 1 1 2 2 1 0 2 5> 2 1 1 3 1 2 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 MN> 0.9 0.6 1.3 1.3 0.8 0.8 0.6 0.3 0.7 0.5 --B-ADENOMA, HEPATOCELLULAR -> 35 26 26 31 30 37 37 33 28 27 +> 1 2 1 2 0 0 0 2 1 1 TL> 36 28 27 33 30 37 37 35 29 28 --B-CHOLANGIOMA -> 36 28 27 32 30 37 37 35 29 28 +> 0 0 0 1 0 0 0 0 0 0 TL> 36 28 27 33 30 37 37 35 29 28 --M-CARCINOMA, HEPATOCELLULAR -> 36 28 27 33 30 37 37 35 29 28 TL> 36 28 27 33 30 37 37 35 29 28 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 35 27 26 32 29 37 37 35 29 27 +> 1 1 1 1 1 0 0 0 0 1 TL> 36 28 27 33 30 37 37 35 29 28 667 TABLE 51 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------ ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) .................. ....................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --CYST -> 64 55 55 63 8 40 1 1> 0 0 0 0 0 0 0 2> 0 0 0 2 0 0 0 3> 0 0 0 0 0 0 0 4> 1 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.1 0.1 oo oo oo oo oo oo o o oo oo oo oo o o --CYST, BILIARY -> 65 55 55 65 3> 0 0 0 0 TL> 65 55 55 65 MN> 8 40 00 8 40 1 0 1 --DEGENERATION, CYSTIC -> 59 47 41 47 1> 3 1 1 5 2> 0 2 3 8 3> 2 4 7 4 4> 1 1 3 1 TL> 65 55 55 65 MN> 0.2 0.4 0.7 0.6 8 33 02 04 01 00 8 40 1 0 0 0 0 1 o o ro o o o 668 TABLE 51 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ----------------------------------- ----------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ....................... .................. NUMBER EXAMINED: 65 55 55 65 8 40 1 --FIBROSIS -> 65 55 54 65 8 38 1 1> 0 0 1 0 0 1 0 2> 0 0 0 0 0 0 0 3> 0 0 0 0 0 1 0 TL> 65 55 55 65 8 40 1 MN> 0.1 oo oo o o oo oo oo oo oo oo oo oo oo oo --FIBROSIS, CAPSULAR -> 65 54 55 65 1> 0 1 0 0 3> 0 0 0 0 TL> 65 55 55 65 MN> 8 40 00 00 8 40 1 0 0 1 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL o o o o o o o o o o o o -> 64 54 54 65 2> 0 0 1 0 3> 1 0 0 0 4> 0 1 0 0 TL> 65 55 55 65 MN> 0.1 8 40 00 00 00 8 40 1 0 0 0 1 669 TABLE 51 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ** FROM PREVIOUS PAGE ** LIVER (LI) ....................... .... NUMBER EXAMINED: 65 55 55 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 57 43 44 1> 2 3 2 2> 3 5 2 3> 3 3 4 4> 0 0 2 5> 0 1 1 TL> 65 55 55 MN> 0.5 0.6 65 59 0 1 3 2 0 65 8 40 8 38 00 00 02 00 00 8 40 0.2 1 1 0 0 0 0 0 1 o oo o oo oo oo o oo o oo ro o oo oo oo oo oo ro o o o oo --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 64 55 53 64 1> 1 0 2 0 2> 0 0 0 0 4> 0 0 0 1 TL> 65 55 55 65 MN> 0.1 8 40 00 00 00 8 40 1 0 0 0 1 --HEMATOPOIESIS, EXTRAMEDULLARY -> 64 55 55 64 1> 1 0 0 1 2> 0 0 0 0 TL> 65 55 55 65 MN> 8 40 00 00 8 40 1 0 0 1 670 TABLE 51 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 -------------------------------- -------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .................... ..................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --HEMORRHAGE -> 62 52 52 64 3 33 1 1> 2 2 1 0 4 4 0 2> 1 1 1 1 0 0 0 3> 0 0 1 0 0 3 0 4> 0 0 0 0 1 0 0 TL> 65 55 55 65 8 40 1 MN> 0.1 0.1 0.1 0.0 1.0 0.3 0.0 --HYPERPLASIA, BILE DUCT -> 56 46 47 54 1> 6 9 6 9 2> 3 0 2 2 3> 0 0 0 0 TL> 65 55 55 65 MN> 0.2 0.2 0.2 0.2 8 35 03 01 01 8 40 0.2 1 0 0 0 1 o o o o o o o o --HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR -> 65 1> 0 2> 0 3> 0 4> 0 TL> 65 MN> 54 52 43 1 38 0 111000 019010 016510 0062 01 55 55 65 8 40 1 0.1 0.9 2.9 0.1 4.0 671 TABLE 51 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M BE R -O F -A N IMA SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------------------ ---------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .................................... ..... NUMBER EXAMINED: 65 55 55 65 8 40 1 --INFLAMMATION, ACUTE -> 65 55 55 65 8 39 1 1> 0 0 0 0 0 1 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 47 35 32 45 8 31 1 1> 17 20 22 20 0 9 0 2> 1 0 1 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.3 0.4 0.4 0.3 0.0 0.2 0.0 --INFLAMMATION, CHRONIC-ACTIVE -> 65 55 54 65 8 40 1 2> 0 0 0 0 0 0 0 3> 0 0 1 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.1 0.0 0.0 0.0 0.0 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 65 53 54 63 8 40 1 2> 0 1 0 0 0 0 0 3> 0 0 0 0 0 0 0 4> 0 0 0 2 0 0 0 5> 0 1 1 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.1 0.1 0.1 0.0 0.0 0.0 Covance 6329-212 3M T-6316.1 PAGE: 5 672 TABLE 51 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------- --------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ......................... ................ NUMBER EXAMINED: 65 55 55 65 8 40 1 --MINERALIZATION -> 65 55 55 65 8 40 1 2> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 o o o o o o o o MN> oo ro o ro o oo o oo o oo oo oo o oo o oo o oo o oo --NECROSIS, HEPATOCELLULAR -> 64 52 51 1> 0 1 1 2> 0 1 2 3> 1 1 0 4> 0 0 0 5> 0 0 1 TL> 65 55 55 MN> 0.1 0.2 63 2 11 11 03 01 00 65 8 2.0 36 0 0 3 1 0 40 0 0 0 1 0 0 1 oo oo --NECROSIS, INDIVIDUAL HEPATOCYTE -> 65 55 55 65 1> 0 0 0 0 TL> 65 55 55 65 MN> 8 40 00 8 40 1 0 1 --PIGMENT o o -> 65 54 54 64 1> 0 0 0 0 2> 0 1 1 1 TL> 65 55 55 65 MN> o o o 8 39 01 00 8 40 o o o 1 0 0 1 673 TABLE 51 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------ ---------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) .................. ....................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --PIGMENT, HEPATOCELLULAR -> 65 55 55 65 8 40 1 3> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> oo o o oo o o oo oo oo o o oo o o oo o o oo o o oo oo oo oo --PIGMENT, KUPFFER CELL -> 65 55 55 65 1> 0 0 0 0 TL> 65 55 55 65 MN> 8 40 00 8 40 1 0 1 --STASIS, BILE o o -> 65 55 55 64 1> 0 0 0 1 2> 0 0 0 0 3> 0 0 0 0 TL> 65 55 55 65 MN> o o 8 39 00 00 01 8 40 0.1 1 0 0 0 1 --TELANGIECTASIS -> 63 1> 1 2> 0 3> 1 TL> 65 MN> 0.1 48 52 21 21 31 55 55 0.1 65 0 0 0 65 o o 8 40 00 00 00 8 40 1 0 0 0 1 o o o ro oo o o 674 TABLE 51 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 --------------------------------- ------------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ..................... .................... NUMBER EXAMINED: 65 55 55 65 8 40 1 --THROMBUS -> 65 54 55 65 8 40 1 1> 0 0 0 0 0 0 0 2> 0 1 0 0 0 0 0 3> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 MN> 0.0 0.0 0.0 0.0 0.0 0.0 0.0 --VACUOLATION, HEPATOCELLULAR -> 48 37 32 26 7 29 1> 6 7 9 12 1 4 2> 4 5 2 10 0 2 3> 4 4 8 11 0 3 4> 1 1 3 2 0 1 5> 2 1 1 4 0 1 TL> 65 55 55 65 8 40 MN> 0.6 0.7 1.0 1.4 0.1 0.7 o o 1 0 0 0 0 0 1 --B-ADENOMA, HEPATOCELLULAR -> 63 51 53 60 +> 2 4 2 5 TL> 65 55 55 65 8 40 00 8 40 1 0 1 --B-CHOLANGIOMA -> 65 55 55 64 +> 0 0 0 1 TL> 65 55 55 65 8 40 00 8 40 1 0 1 675 TABLE 51 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: -MALE- GROUP: -1- -2- -3- -4- -5- -6- -7- ORGAN/TISSUE EXAMINED NUMBER: 70 60 60 70 70 40 40 ------------------------------------ ---------------------------------- -=- -=- -=- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................. NUMBER EXAMINED: 65 55 55 65 8 40 1 --M-CARCINOMA, HEPATOCELLULAR -> 65 55 55 65 8 40 1 +> 0 0 0 0 0 0 0 TL> 65 55 55 65 8 40 1 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 63 54 54 64 +> 2 1 1 1 TL> 65 55 55 65 8 39 01 8 40 1 0 1 676 APPENDIX 1 P ro to c o l D e v iatio n s Covance 6329-212 3M T-6316.1 677 Protocol Deviations Protocol. Test Material. Purity. 98.1% N-EtFOSE (w/w). Covance 6329-212 3M T-6316.1 Actual Procedure. The purity of the test material, as determined by Centre Analytical Laboratories, Inc. on 26 November 2000, was 97.4%. Protocol. Animals. Weight at Initiation of Treatment. Animals will weigh 100 to 300 g at initiation. Actual Procedure. Six animals weighed between 3 01to310gat initation. Protocol. Husbandry. Water. Water will be provided a d libitum. Actual Procedure. During the a.m. clinical observations on August 18, 1998, it was noted that animals in Cage Rack 3 were without water. Animals appeared in good condition. Animals involved were not specified in the data. During the a.m. clinical observations on June 25, 1998, it was noted that Group 4 males were without water. Animals appeared in good condition. Protocol. Dosing Procedures. Method of Administration. Animals will be offered diet a d libitum, unless otherwise specified. Actual Procedure. Animal No. C91073, Group 6 male, was found without a feed jar on March 8, 1999. Animal No. C90899, Group 3 male, was found without a feed jar on December 8, 1999. Animal No. C91336, Group 4 female, was found with an empty feed jar on May 2, 1999. Animal No. C90934, Group 4 male, was found without a feed jar on January 10, 2000. Protocol. Dosing Procedures. Dose Preparation. All dose preparations will be stored at room temperature. 678 Protocol Deviations (Continued) Covance 6329-212 3M T-6316.1 Actual Procedure. D i e t s m i x e d f o r W e e k s 4 t h r o u g h 7 w e r e s t o r e d i n a r e f r i g e r a t o r f o r a p p ro x im a te ly 1 6 .5 h o u rs d u rin g W e e k 4. Protocol. D o s i n g P r o c e d u r e s . R e t e n t i o n S a m p l e s . S a m p l e s w i l l b e c o l l e c t e d f r o m e a c h d o se p rep aratio n . Actual Procedure. S a m p l e s f o r t h e W e e k 4 t h r o u g h 7 p r e p a r a t i o n w e r e n o t c o l l e c t e d u n til F e b ru a ry 2 3 , 1998 (W ee k 5) fro m th e d iet th a t w a s in a d v e rte n tly sto re d in th e re frig e ra to r fo r a p p ro x im a te ly 1 6 .5 h o u rs. Protocol. D o s i n g P r o c e d u r e s . R e t e n t i o n S a m p l e s . U n u s e d r e t e n t i o n s a m p l e s w i l l b e d isc a rd e d a t lea st 1 m o n th a fte r c o m p le tio n o f th e in life p h ase. Actual Procedure. U n u s e d r e t e n t i o n s a m p l e s w e r e d i s c a r d e d o n M a r c h 2 1 , 2 0 0 0 . G ro u p 1 re te n tio n sa m p le s fo r W ee k s 64 th ro u g h 6 7 w e re n o t lo cated . Protocol. D o s e A n a l y s e s . H o m o g e n e i t y . S a m p l e s w i l l b e a n a l y z e d i n t r i p l i c a t e . Actual Procedure. S a m p l e s w e r e a n a l y z e d i n d u p l i c a t e f o r t h e m i x o n A u g u s t 2 6 , 1 9 9 9 . Protocol. O b s e r v a t i o n o f A n i m a l s . C l i n i c a l O b s e r v a t i o n s . E a c h a n i m a l w i l l b e o b s e r v e d tw ic e d a ily (a .m . a n d p .m .). Actual Procedure. C o m p l e t i o n o f t h e p . m . o b s e r v a t i o n s o n A u g u s t 1 0 , 1 9 9 9 w a s n o t d o c u m e n te d in th e d ata. Protocol. O b s e r v a t i o n o f A n i m a l s . C l i n i c a l O b s e r v a t i o n s . A n i m a l s w i l l b e r e m o v e d fro m c ag e s w e e k ly an d a b n o rm a l fin d in g s w ill b e re co rd e d . 679 Protocol Deviations (Continued) Covance 6329-212 3M T-6316.1 Actual Procedure. D u r i n g t h e w e e k l y d a t a c o l l e c t i o n , A n i m a l N o . C 9 1 4 6 8 , G r o u p 6 fem ale, h ad sev ere c o n v u lsio n s an d d ied (A p ril 12, 1999). T h ese o b se rv atio n s w e re n o t d o cu m en ted . Protocol. O b s e r v a t i o n o f A n i m a l s . B o d y W e i g h t s . B o d y w e i g h t s w i l l b e c o l l e c t e d w e ek ly fo r W ee k s 1 th ro u g h 17. Actual Procedure. B o d y w e i g h t f o r W e e k 5 f o r A n i m a l N o . C 9 1 0 1 0 , G r o u p 5 m a l e , w e re ex clu d ed d u e to an a p p are n t w e ig h in g erro r. Protocol. O b s e r v a t i o n o f A n i m a l s . F o o d C o n s u m p t i o n . F o o d c o n s u m p t i o n w i l l b e m ea su re d w e ek ly fo r W ee k s 1 th ro u g h 16. Actual Procedure. F o o d c o n s u m p t i o n f o r A n i m a l N o . C 9 0 9 9 5 , G r o u p 5 m a l e , w a s ex clu d ed fo r W e e k 2 d u e to an a p p are n t w e ig h in g erro r. Protocol. C l i n i c a l P a t h o l o g y . B l o o d F i l m s . " B l o o d f i l m s w i l l b e t a k e n a s p a r t o f t h e n e cro p sy p ro c e d u re ." Actual Procedure. A b l o o d f i l m w a s n o t t a k e n f o r A n i m a l N o . C 9 1 2 1 1 , G r o u p 2 fem ale, at th e W e e k 105 sacrifice. Protocol. S e r u m A n a l y s e s . M e t h o d o f C o l l e c t i o n . A p p r o x i m a t e l y 3 m L o f b l o o d w i l l b e c o lle c te d at th e W e e k 105 sa c rifice fo r p o ssib le fu tu re seru m an aly ses. Actual Procedure. A s a m p l e w a s n o t o b t a i n e d f o r A n i m a l N o . C 9 1 2 1 1 , G r o u p 2 f e m a l e , at th e W e e k 105 sacrifice. 680 Protocol Deviations (Continued) Covance 6329-212 3M T-6316.1 Protocol. H e p a t o c e l l u l a r P r o l i f e r a t i o n a n d B i o c h e m i c a l A n a l y s e s . P a l m i t o y l - C o A O x id a se T issu e C o lle c tio n an d A n aly ses. A 5 0 0 -m g sam p le o f th e rig h t late ra l lo b e o f th e liv e r w ill b e c o llected . Actual Procedure. A 5 0 0 - m g s a m p l e f r o m t h e r i g h t m e d i a n l o b e w a s c o l l e c t e d f o r t h e fo llo w in g a n im a ls at W e e k 14. A n im al N o s. C 9 0 8 3 1 , C 9 0 7 9 7 (G ro u p 2 m ales), C 9 1 2 8 1 (G ro u p 3 fe m a le ), C 9 0 7 8 0 (G ro u p 1 m ale ), C 9 0 9 6 0 (G ro u p 4 m ale ), C 9 1 2 4 1 (G ro u p 2 fe m a le ), a n d C 9 1 3 7 0 (G ro u p 4 fe m a le ). Protocol. T e r m i n a t i o n . U n c h e d u l e d S a c r i f i c e s a n d D e a t h s . A n i m a l s s a c r i f i c e d d u r i n g o r a fte r W e e k 13 w ill b e a n e sth e tiz e d w ith c a rb o n d io x id e. Actual Procedure. A n i m a l N o s . C 9 0 8 0 6 a n d C 9 0 8 0 8 ( G r o u p 2 m a l e ) a n d A n i m a l N o . C 9 1 4 8 4 (G ro u p 6 fe m a le ) w e re sa c rifice d u sin g so d iu m p e n to b a rb ita l. Protocol. T e r m i n a t i o n . U n s c h e d u l e d S a c r i f i c e s a n d D e a t h s . A n i m a l s w i l l b e w e i g h e d p rio r to e x san g u in atio n . Actual Procedure. T h e f o l l o w i n g a n i m a l s w e r e n o t w e i g h e d : A n i m a l N o s . C 9 1 1 3 9 , G ro u p 1 fe m a le ; C 9 0 8 0 6 , G ro u p 2 m ale, C 9 1 2 0 3 , G ro u p 2 fe m a le ; C 9 1 2 1 0 , G ro u p 2 fem ale; C 9 0 8 4 9 , G ro u p 3 m ale; C 9 0 9 4 6 , G ro u p 4 m ale; C 9 0 9 4 9 , G ro u p 4 m ale; C 9 1 3 5 8 , G ro u p 4 fe m a le ; C 9 1 0 2 9 , G ro u p 5 m ale; a n d C 9 1 0 6 8 , G ro u p 6 m ale. Protocol. P o s t m o r t e m P r o c e d u r e s . O r g a n W e i g h t s S p e c i f i e d o r g a n s w i l l b e w e i g h e d a t th e W e e k 8 sacrifice. Actual Procedure. D u e t o a n a p p a r e n t w e i g h i n g e r r o r , o r g a n w e i g h t s f o r t h e l e f t th y ro id /p a ra th y ro id fo r A n im al N o s. C 9 0 7 5 6 (G ro u p 1 m ale), C 9 1 0 1 7 (G ro u p 5 m ale), an d C 9 1 3 9 3 (G ro u p 5 fe m a le ) w e re ex clu d ed . 681 Protocol Deviations (Continued) Covance 6329-212 3M T-6316.1 Protocol. P o s t m o r t e m P r o c e d u r e s . T i s s u e P r e s e r v a t i o n . L e s i o n s w i l l b e c o l l e c t e d f r o m e a c h a n im a l th a t d ies a t an u n sc h e d u le d in terv al. Actual Procedure. T h e l a s t i n l i f e o b s e r v a t i o n f o r A n i m a l N o . C 9 1 2 6 8 , G r o u p 3 f e m a l e , o f c ru ste d fo o tp a d fo r th e le ft h in d fo o t w a s c o n firm e d a t n e cro p sy . T h is le sio n w a s n o t c o lle cted an d th e rig h t h in d fo o t w a s in ad v e rte n tly c o llected . Protocol. P o s t m o r t e m P r o c e d u r e s . T i s s u e P r e s e r v a t i o n . M a n d i b u l a r s a l i v a r y g l a n d w i l l b e c o lle cted , p re serv e d , an d e x am in ed m ic ro sc o p ic a lly fo r a n im a ls in G ro u p 4. Actual Procedure. T w o s e t s o f m a n d i b u l a r s a l i v a r y g l a n d s w e r e n o t e d i n t h e w e t t i s s u e c o lle c tio n fo r A n im a l N o . C 9 1 3 1 2 , G ro u p 4 fem ale. N e ith e r set w a s su b m itte d fo r m ic ro sc o p ic ex am in atio n . Protocol. P o s t m o r t e m P r o c e d u r e s . T i s s u e P r e s e r v a t i o n . T h e f o l l o w i n g t i s s u e s f r o m e a c h a n im a l w ill b e p re se rv e d in 1 0% n e u tra l-b u ffe rd fo rm alin . Actual Procedure. S o m e t i s s u e s , r e q u i r e d b y p r o t o c o l , w e r e n o t a v a i l a b l e f o r p re se rv a tio n o r h isto p ath o lo g ic e x am in atio n . M issin g tissu e s are liste d w ith a p p ro p ria te c o m m e n ts in th e p a th o lo g y d a ta sh e e ts fo r in d iv id u a l a n im a ls. S u m m a ry ta b le s d o n o t in clu d e th e m as h a v in g b e e n ex am in ed . T h e se d e v ia tio n s a re n o t e x p e c te d to h a v e a ffe c te d th e re su lts o f th e study. 682 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-212 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e III o f X II Page 683 of4873 Covance 6329-212 3M T-6316.1 VOLUME III OF XII CONTENTS Page A P P E N D I X 2 ..........................................................................................................................................................................................6 8 5 I n d i v i d u a l A n i m a l F a t e D a t a ..............................................................................................................................................6 8 6 I n d i v i d u a l C l i n i c a l O b s e r v a t i o n s ....................................................................................................................................7 1 1 684 APPENDIX 2 In d iv id u a l A n im al F a te D a ta In d iv id u a l C lin ic al O b se rv a tio n s Covance 6329-212 3M T-6316.1 685 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C90711 C90712 C90713 C90714 C90715 C90716 C90717 C90718 C90719 C90720 C90721 C90722 C90723 C90724 C90725 C90726 C90727 C90728 C90729 C90730 C90731 C90732 C90733 C90734 C90735 C90736 C90737 C90738 C90739 C90740 C90741 C90742 C90743 C90744 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 07/26/99 01/26/00 12/10/99 01/27/99 11/05/99 01/25/00 03/20/98 03/20/98 02/18/98 10/05/99 11/26/98 01/25/00 03/22/99 01/25/00 02/18/98 01/26/00 11/29/99 01/25/00 11/01/99 05/20/99 04/29/98 02/18/98 11/22/99 08/02/99 01/25/00 06/13/99 03/20/98 12/15/99 01/09/00 04/26/99 12/22/99 01/25/00 01/26/00 09/21/99 547 731 684 367 649 730 54 54 24 618 305 730 421 730 24 731 673 730 645 480 94 24 666 554 730 504 54 689 714 456 696 730 731 604 WEEK OF STUDY 79 105 98 53 93 105 8 8 4 89 44 105 61 105 4 105 97 105 93 69 14 4 96 80 105 72 8 99 102 66 100 105 105 87 PAGE: 1 686 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C90745 C90746 C90747 C90748 C90749 C90750 C90751 C90752 C90753 C90754 C90755 C90756 C90757 C90758 C90759 C90760 C90761 C90762 C90763 C90764 C90765 C90766 C90767 C90768 C90769 C90770 C90771 C90772 C90773 C90774 C90775 C90776 C90777 C90778 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 07/12/99 04/29/98 01/25/00 04/29/98 11/03/99 01/26/00 01/27/99 01/27/99 02/18/98 01/27/99 12/21/99 03/20/98 05/28/99 01/26/00 09/14/99 09/10/99 11/18/99 07/27/99 09/21/99 09/04/99 01/25/00 04/10/99 01/27/99 04/29/98 03/20/98 10/27/99 11/11/99 03/29/99 04/25/99 06/09/99 02/18/98 12/22/99 09/29/99 01/27/00 533 94 730 94 647 731 367 367 24 367 695 54 488 731 597 593 662 548 604 587 730 440 367 94 54 640 655 428 455 500 24 696 612 732 WEEK OF STUDY 77 14 105 14 93 105 53 53 4 53 100 8 70 105 86 85 95 79 87 84 105 63 53 14 8 92 94 62 65 72 4 100 88 105 PAGE: 2 687 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C90779 C90780 C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 C90812 1 1 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 11/03/99 04/29/98 01/25/00 01/26/00 01/26/00 11/08/99 01/26/00 11/02/99 01/26/00 01/27/00 02/18/98 08/09/99 01/27/00 02/25/99 07/19/99 01/26/00 03/01/99 03/13/99 04/29/98 12/29/99 01/25/00 08/26/99 12/11/99 08/10/99 11/07/99 01/26/00 03/24/99 09/21/98 04/29/98 05/31/99 01/27/00 01/26/00 01/25/00 01/25/00 647 94 730 731 731 652 731 646 731 732 24 561 732 396 540 731 400 412 94 703 730 578 685 562 651 731 423 239 94 491 732 731 730 730 WEEK OF STUDY 93 14 105 105 105 94 105 93 105 105 4 81 105 57 78 105 58 59 14 101 105 83 98 81 93 105 61 35 14 71 105 105 105 105 PAGE: 3 688 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 C90841 C90842 C90843 C90844 C90845 C90846 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE DATE OF DAY OF DEATH STUDY 03/18/98 02/18/98 01/25/00 09/15/99 02/18/98 04/29/98 10/28/99 05/25/99 12/21/99 07/12/99 01/25/00 08/09/99 12/11/99 10/29/99 05/11/99 08/08/99 01/26/00 01/26/00 04/29/98 01/26/00 02/18/98 02/18/98 01/27/00 04/29/98 06/21/99 01/26/00 12/19/99 01/25/00 12/03/99 01/26/00 04/29/98 01/26/00 07/26/99 02/18/98 52 24 730 598 24 94 641 485 695 533 730 561 685 642 471 560 731 731 94 731 24 24 732 94 512 731 693 730 677 731 94 731 547 24 WEEK OF STUDY 8 4 105 86 4 14 92 70 100 77 105 81 98 92 68 80 105 105 14 105 4 4 105 14 74 105 99 105 97 105 14 105 79 4 PAGE: 4 689 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH A UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE DATE OF DAY OF DEATH STUDY 12/13/99 07/05/99 07/19/99 01/26/00 01/25/00 04/29/98 02/18/98 01/26/00 01/27/00 01/25/00 01/14/00 01/25/00 05/19/99 01/25/00 06/04/99 05/17/99 04/29/98 01/26/00 01/27/00 02/18/98 01/26/00 06/17/99 01/25/00 08/02/99 01/25/00 10/24/99 06/17/99 05/03/99 10/19/99 02/15/99 04/29/98 01/29/99 08/09/99 04/29/98 687 526 540 731 730 94 24 731 732 730 719 730 479 730 495 477 94 731 732 24 731 508 730 554 730 637 508 463 632 386 94 369 561 94 WEEK OF STUDY 99 76 78 105 105 14 4 105 105 105 103 105 69 105 71 69 14 105 105 4 105 73 105 80 105 91 73 67 91 56 14 53 81 14 PAGE: 5 690 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 01/26/00 01/26/00 01/27/00 01/26/00 01/26/00 10/18/99 05/17/99 08/09/99 01/25/00 02/18/98 02/18/98 07/12/99 01/26/00 12/10/99 11/13/99 06/07/99 11/17/99 04/01/99 01/17/00 01/26/00 06/21/99 01/27/99 12/29/99 01/25/00 04/29/98 01/08/00 04/29/98 01/25/00 01/27/99 01/27/00 01/26/00 08/16/99 01/27/99 12/22/99 731 731 732 731 731 631 477 561 730 24 24 533 731 684 657 498 661 431 722 731 512 367 703 730 94 713 94 730 367 732 731 568 367 696 WEEK OF STUDY 105 105 105 105 105 91 69 81 105 4 4 77 105 98 94 72 95 62 104 105 74 53 101 105 14 102 14 105 53 105 105 82 53 100 PAGE: 6 691 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 11/22/99 01/26/00 04/29/98 01/27/99 07/30/99 01/22/00 04/29/98 04/12/99 09/18/99 01/27/99 11/02/99 01/25/00 01/25/00 01/25/00 05/17/99 10/11/99 12/06/99 01/27/99 01/25/00 01/14/00 02/18/98 12/31/99 01/10/00 05/21/99 02/18/98 04/12/99 09/27/99 02/18/98 10/26/99 01/26/00 11/30/98 07/19/99 01/26/00 01/26/00 666 731 94 367 551 727 94 442 601 367 646 730 730 730 477 624 680 367 730 719 24 705 715 481 24 442 610 24 639 731 309 540 731 731 WEEK OF STUDY 96 105 14 53 79 104 14 64 86 53 93 105 105 105 69 90 98 53 105 103 4 101 103 69 4 64 88 4 92 105 45 78 105 105 PAGE: 7 692 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 C90971 C90972 C90973 C90974 C90975 C90976 C90977 C90978 C90979 C90980 C90981 C90982 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 5 5 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION <OF DEATH M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED DATE OF DAY OF DEATH STUDY 04/21/98 01/27/99 01/27/99 03/12/99 01/25/00 05/19/99 01/27/00 01/26/00 01/27/99 11/12/99 09/17/99 04/29/98 02/18/98 06/01/99 06/18/99 06/24/99 01/26/00 02/18/98 01/27/99 01/25/00 09/10/99 12/23/99 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 86 367 367 411 730 479 732 731 367 656 600 94 24 492 509 515 731 24 367 730 593 697 54 54 54 54 54 54 54 54 54 54 54 54 WEEK OF STUDY 13 53 53 59 105 69 105 105 53 94 86 14 4 71 73 74 105 4 53 105 85 100 8 8 8 8 8 8 8 8 8 8 8 8 PAGE: 8 693 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C90983 C90984 C90985 C90986 C90987 C90988 C90989 C90990 C90991 C90992 C90993 C90994 C90995 C90996 C90997 C90998 C90999 C91000 C91001 C91002 C91003 C91004 C91005 C91006 C91007 C91008 C91009 C91010 C91011 C91012 C91013 C91014 C91015 C91016 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 1 SCHEDULED FIRST INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 2 SCHEDULED SECOND INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED D UNSCHEDULED UNSCHEDULED DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 2 SCHEDULED SECOND INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED D UNSCHEDULED UNSCHEDULED DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 1 SCHEDULED FIRST INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED DATE OF DAY OF DEATH STUDY 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/19/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/11/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 54 24 54 54 54 54 54 54 54 54 53 54 54 54 54 54 54 54 54 54 45 54 24 54 54 54 54 54 54 54 54 54 54 54 WEEK OF STUDY 8 4 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 7 8 4 8 8 8 8 8 8 8 8 8 8 8 PAGE: 9 694 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C91017 C91018 C91019 C91020 C91021 C91022 C91023 C91024 C91025 C91026 C91027 C91028 C91029 C91030 C91031 C91032 C91033 C91034 C91035 C91036 C91037 C91038 C91039 C91040 C91041 C91042 C91043 C91044 C91045 C91046 C91047 C91048 C91049 C91050 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 1 SCHEDULED FIRST INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED M UNSCHEDULED UNSCHEDULED DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 1 SCHEDULED FIRST INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 1 SCHEDULED FIRST INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 03/15/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/22/99 11/06/99 09/07/99 04/13/99 01/13/99 03/10/98 01/27/00 08/02/99 11/16/98 01/14/00 54 54 54 54 54 54 54 24 54 54 54 54 49 54 54 54 24 54 54 54 24 54 54 54 421 650 590 443 353 44 732 554 295 719 WEEK OF STUDY 8 8 8 8 8 8 8 4 8 8 8 8 7 8 8 8 4 8 8 8 4 8 8 8 61 93 85 64 51 7 105 80 43 103 PAGE: 10 695 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C91051 C91052 C91053 C91054 C91055 C91056 C91057 C91058 C91059 C91060 C91061 C91062 C91063 C91064 C91065 C91066 C91067 C91068 C91069 C91070 C91071 C91072 C91073 C91074 C91075 C91076 C91077 C91078 C91079 C91080 C91081 C91082 C91083 C91084 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6 7 7 7 7 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC U SCHEDULED FIRST POST- RECOV SAC U SCHEDULED FIRST POST- RECOV SAC U SCHEDULED FIRST POST- RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED DATE OF DAY OF DEATH STUDY 10/05/99 05/10/99 08/28/99 01/27/00 08/17/99 01/27/00 06/19/99 08/23/99 08/24/99 01/27/00 10/14/99 11/01/99 12/23/99 12/05/99 01/27/00 09/15/99 12/22/99 04/13/98 09/02/99 01/27/00 01/27/00 01/27/00 01/27/00 01/06/00 07/19/99 01/27/00 09/05/99 11/05/99 09/07/99 12/07/99 03/20/98 03/20/98 03/20/98 03/20/98 618 470 580 732 569 732 510 575 576 732 627 645 697 679 732 598 696 78 585 732 732 732 732 711 540 732 588 649 590 681 54 54 54 54 WEEK OF STUDY 89 68 83 105 82 105 73 83 83 105 90 93 100 97 105 86 100 12 84 105 105 105 105 102 78 105 84 93 85 98 8 8 8 8 PAGE: 11 696 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP C91085 C91086 C91087 C91088 C91089 C91090 C91091 C91092 C91093 C91094 C91095 C91096 C91097 C91098 C91099 C91100 C91101 C91102 C91103 C91104 C91105 C91106 C91107 C91108 C91109 C91110 C91111 C91112 C91113 C91114 C91115 C91116 C91117 C91118 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED D UNSCHEDULED UNSCHEDULED DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED DATE OF DAY OF DEATH STUDY 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/05/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 54 54 54 54 54 54 54 54 54 54 54 54 54 54 39 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 WEEK OF STUDY 8 8 8 8 8 8 8 8 8 8 8 8 8 8 6 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 PAGE: 12 697 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91119 7 MALE C91120 7 MALE C91121 1 FEMALE C91122 1 FEMALE C91123 1 FEMALE C91124 1 FEMALE C91125 1 FEMALE C91126 1 FEMALE C91127 1 FEMALE C91128 1 FEMALE C91129 1 FEMALE C91130 1 FEMALE C91131 1 FEMALE C91132 1 FEMALE C91133 1 FEMALE C91134 1 FEMALE C91135 1 FEMALE C91136 1 FEMALE C91137 1 FEMALE C91138 1 FEMALE C91139 1 FEMALE C91140 1 FEMALE C91141 1 FEMALE C91142 1 FEMALE C91143 1 FEMALE C91144 1 FEMALE C91145 1 FEMALE C91146 1 FEMALE C91147 1 FEMALE C91148 1 FEMALE C91149 1 FEMALE C91150 1 FEMALE C91151 1 FEMALE C91152 1 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 4 SCHEDULED FOURTH INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE DATE OF DAY OF DEATH STUDY 03/20/98 03/20/98 01/27/99 01/27/99 12/09/99 01/27/99 11/27/99 03/20/98 01/25/00 07/02/99 04/29/98 07/13/99 01/25/00 10/05/99 01/27/99 06/28/99 02/18/98 10/11/99 01/10/00 01/27/00 04/02/99 03/20/98 08/06/99 06/14/99 02/18/98 01/26/00 03/20/98 06/28/99 07/14/99 01/25/00 09/27/99 07/05/99 06/21/99 01/27/99 54 54 367 367 683 367 671 54 730 523 94 534 730 618 367 519 24 624 715 732 432 54 558 505 24 731 54 519 535 730 610 526 512 367 WEEK OF STUDY 8 8 53 53 98 53 96 8 105 75 14 77 105 89 53 75 4 90 103 105 62 8 80 73 4 105 8 75 77 105 88 76 74 53 PAGE: 13 698 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91153 1 FEMALE C91154 1 FEMALE C91155 1 FEMALE C91156 1 FEMALE C91157 1 FEMALE C91158 1 FEMALE C91159 1 FEMALE C91160 1 FEMALE C91161 1 FEMALE C91162 1 FEMALE C91163 1 FEMALE C91164 1 FEMALE C91165 1 FEMALE C91166 1 FEMALE C91167 1 FEMALE C91168 1 FEMALE C91169 1 FEMALE C91170 1 FEMALE C91171 1 FEMALE C91172 1 FEMALE C91173 1 FEMALE C91174 1 FEMALE C91175 1 FEMALE C91176 1 FEMALE C91177 1 FEMALE C91178 1 FEMALE C91179 1 FEMALE C91180 1 FEMALE C91181 1 FEMALE C91182 1 FEMALE C91183 1 FEMALE C91184 1 FEMALE C91185 1 FEMALE C91186 1 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 08/09/99 07/20/99 04/29/98 06/28/99 01/26/00 11/08/99 01/25/00 04/29/98 03/22/99 03/20/98 03/20/98 11/15/99 02/18/98 01/26/00 01/12/00 09/05/99 02/18/98 10/05/99 02/18/98 01/25/00 03/05/99 04/29/98 09/17/99 01/25/00 11/08/99 10/18/99 12/15/98 04/16/99 04/29/98 09/27/99 01/25/00 10/16/99 05/04/99 12/06/99 561 541 94 519 731 652 730 94 421 54 54 659 24 731 717 588 24 618 24 730 404 94 600 730 652 631 324 446 94 610 730 629 464 680 WEEK OF STUDY 81 78 14 75 105 94 105 14 61 8 8 95 4 105 103 84 4 89 4 105 58 14 86 105 94 91 47 64 14 88 105 90 67 98 PAGE: 14 699 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91187 1 FEMALE C91188 1 FEMALE C91189 1 FEMALE C91190 1 FEMALE C91191 2 FEMALE C91192 2 FEMALE C91193 2 FEMALE C91194 2 FEMALE C91195 2 FEMALE C91196 2 FEMALE C91197 2 FEMALE C91198 2 FEMALE C91199 2 FEMALE C91200 2 FEMALE C91201 2 FEMALE C91202 2 FEMALE C91203 2 FEMALE C91204 2 FEMALE C91205 2 FEMALE C91206 2 FEMALE C91207 2 FEMALE C91208 2 FEMALE C91209 2 FEMALE C91210 2 FEMALE C91211 2 FEMALE C91212 2 FEMALE C91213 2 FEMALE C91214 2 FEMALE C91215 2 FEMALE C91216 2 FEMALE C91217 2 FEMALE C91218 2 FEMALE C91219 2 FEMALE C91220 2 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 06/18/99 01/26/00 11/01/99 01/26/00 12/06/99 04/29/98 10/18/99 01/23/00 12/20/99 08/27/99 01/25/00 02/18/98 11/29/99 09/24/99 01/25/00 09/07/99 07/11/99 03/27/99 02/18/98 06/28/99 01/26/00 06/28/99 06/29/99 07/02/99 01/26/00 05/07/99 07/14/99 12/13/99 02/07/99 01/25/00 05/12/99 11/29/99 09/27/99 01/27/00 509 731 645 731 680 94 631 728 694 579 730 24 673 607 730 590 532 426 24 519 731 519 520 523 731 467 535 687 378 730 472 673 610 732 WEEK OF STUDY 73 105 93 105 98 14 91 104 100 83 105 4 97 87 105 85 76 61 4 75 105 75 75 75 105 67 77 99 54 105 68 97 88 105 PAGE: 15 700 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91221 2 FEMALE C91222 2 FEMALE C91223 2 FEMALE C91224 2 FEMALE C91225 2 FEMALE C91226 2 FEMALE C91227 2 FEMALE C91228 2 FEMALE C91229 2 FEMALE C91230 2 FEMALE C91231 2 FEMALE C91232 2 FEMALE C91233 2 FEMALE C91234 2 FEMALE C91235 2 FEMALE C91236 2 FEMALE C91237 2 FEMALE C91238 2 FEMALE C91239 2 FEMALE C91240 2 FEMALE C91241 2 FEMALE C91242 2 FEMALE C91243 2 FEMALE C91244 2 FEMALE C91245 2 FEMALE C91246 2 FEMALE C91247 2 FEMALE C91248 2 FEMALE C91249 2 FEMALE C91250 2 FEMALE C91251 3 FEMALE C91252 3 FEMALE C91253 3 FEMALE C91254 3 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 01/25/00 02/18/98 04/29/98 12/02/99 09/02/99 09/13/99 01/25/00 11/08/99 11/08/99 02/18/98 10/26/99 01/26/00 04/29/98 04/29/99 01/26/00 01/25/00 02/18/98 05/10/99 03/01/99 07/29/99 04/29/98 01/25/00 01/26/00 05/07/99 11/24/99 09/21/99 06/17/99 04/29/98 11/29/99 06/14/99 02/18/98 01/25/00 01/25/00 01/26/00 730 24 94 676 585 596 730 652 652 24 639 731 94 459 731 730 24 470 400 550 94 730 731 467 668 604 508 94 673 505 24 730 730 731 WEEK OF STUDY 105 4 14 97 84 86 105 94 94 4 92 105 14 66 105 105 4 68 58 79 14 105 105 67 96 87 73 14 97 73 4 105 105 105 PAGE: 16 701 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91255 3 FEMALE C91256 3 FEMALE C91257 3 FEMALE C91258 3 FEMALE C91259 3 FEMALE C91260 3 FEMALE C91261 3 FEMALE C91262 3 FEMALE C91263 3 FEMALE C91264 3 FEMALE C91265 3 FEMALE C91266 3 FEMALE C91267 3 FEMALE C91268 3 FEMALE C91269 3 FEMALE C91270 3 FEMALE C91271 3 FEMALE C91272 3 FEMALE C91273 3 FEMALE C91274 3 FEMALE C91275 3 FEMALE C91276 3 FEMALE C91277 3 FEMALE C91278 3 FEMALE C91279 3 FEMALE C91280 3 FEMALE C91281 3 FEMALE C91282 3 FEMALE C91283 3 FEMALE C91284 3 FEMALE C91285 3 FEMALE C91286 3 FEMALE C91287 3 FEMALE C91288 3 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE DATE OF DAY OF DEATH STUDY 06/22/99 01/26/00 08/27/99 09/17/99 04/08/99 06/18/99 12/15/99 10/05/99 06/07/99 01/25/00 11/09/99 01/18/00 05/04/99 11/29/99 08/26/99 03/08/99 11/09/99 02/18/98 08/09/99 05/30/99 05/20/99 11/29/99 06/07/99 01/25/00 01/26/00 12/07/99 04/29/98 01/25/99 11/03/99 12/20/99 02/18/98 01/25/00 02/18/98 04/29/98 513 731 579 600 438 509 689 618 498 730 653 723 464 673 578 407 653 24 561 490 480 673 498 730 731 681 94 365 647 694 24 730 24 94 WEEK OF STUDY 74 105 83 86 63 73 99 89 72 105 94 104 67 97 83 59 94 4 81 70 69 97 72 105 105 98 14 53 93 100 4 105 4 14 PAGE: 17 702 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91289 3 FEMALE C91290 3 FEMALE C91291 3 FEMALE C91292 3 FEMALE C91293 3 FEMALE C91294 3 FEMALE C91295 3 FEMALE C91296 3 FEMALE C91297 3 FEMALE C91298 3 FEMALE C91299 3 FEMALE C91300 3 FEMALE C91301 3 FEMALE C91302 3 FEMALE C91303 3 FEMALE C91304 3 FEMALE C91305 3 FEMALE C91306 3 FEMALE C91307 3 FEMALE C91308 3 FEMALE C91309 3 FEMALE C91310 3 FEMALE C91311 4 FEMALE C91312 4 FEMALE C91313 4 FEMALE C91314 4 FEMALE C91315 4 FEMALE C91316 4 FEMALE C91317 4 FEMALE C91318 4 FEMALE C91319 4 FEMALE C91320 4 FEMALE C91321 4 FEMALE C91322 4 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE A UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 06/17/99 03/08/99 02/18/98 01/25/00 04/29/98 01/26/00 01/27/00 09/13/99 10/23/99 06/21/99 04/29/98 08/27/99 01/25/00 06/13/99 01/25/00 04/29/98 09/21/99 01/10/00 01/26/00 05/20/99 05/04/99 01/27/00 04/14/99 12/01/99 09/25/99 06/21/99 01/26/00 02/18/98 02/18/98 05/07/98 05/26/99 07/26/99 11/10/99 06/25/98 508 407 24 730 94 731 732 596 636 512 94 579 730 504 730 94 604 715 731 480 464 732 444 675 608 512 731 24 24 102 486 547 654 151 WEEK OF STUDY 73 59 4 105 14 105 105 86 91 74 14 83 105 72 105 14 87 103 105 69 67 105 64 97 87 74 105 4 4 15 70 79 94 22 PAGE: 18 703 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91323 4 FEMALE C91324 4 FEMALE C91325 4 FEMALE C91326 4 FEMALE C91327 4 FEMALE C91328 4 FEMALE C91329 4 FEMALE C91330 4 FEMALE C91331 4 FEMALE C91332 4 FEMALE C91333 4 FEMALE C91334 4 FEMALE C91335 4 FEMALE C91336 4 FEMALE C91337 4 FEMALE C91338 4 FEMALE C91339 4 FEMALE C91340 4 FEMALE C91341 4 FEMALE C91342 4 FEMALE C91343 4 FEMALE C91344 4 FEMALE C91345 4 FEMALE C91346 4 FEMALE C91347 4 FEMALE C91348 4 FEMALE C91349 4 FEMALE C91350 4 FEMALE C91351 4 FEMALE C91352 4 FEMALE C91353 4 FEMALE C91354 4 FEMALE C91355 4 FEMALE C91356 4 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 01/27/99 01/26/00 01/10/00 09/13/99 01/26/00 04/07/99 04/29/98 09/07/99 07/20/99 01/25/00 02/18/98 12/31/99 01/25/00 01/25/00 04/29/98 01/26/00 12/06/99 01/26/00 01/25/00 01/26/00 01/27/99 01/10/00 02/18/98 06/11/99 01/27/99 01/27/99 03/11/99 02/18/98 11/01/99 01/27/99 01/27/99 09/27/99 04/29/98 01/27/00 367 731 715 596 731 437 94 590 541 730 24 705 730 730 94 731 680 731 730 731 367 715 24 502 367 367 410 24 645 367 367 610 94 732 WEEK OF STUDY 53 105 103 86 105 63 14 85 78 105 4 101 105 105 14 105 98 105 105 105 53 103 4 72 53 53 59 4 93 53 53 88 14 105 PAGE: 19 704 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91357 4 FEMALE C91358 4 FEMALE C91359 4 FEMALE C91360 4 FEMALE C91361 4 FEMALE C91362 4 FEMALE C91363 4 FEMALE C91364 4 FEMALE C91365 4 FEMALE C91366 4 FEMALE C91367 4 FEMALE C91368 4 FEMALE C91369 4 FEMALE C91370 4 FEMALE C91371 4 FEMALE C91372 4 FEMALE C91373 4 FEMALE C91374 4 FEMALE C91375 4 FEMALE C91376 4 FEMALE C91377 4 FEMALE C91378 4 FEMALE C91379 4 FEMALE C91380 4 FEMALE C91381 5 FEMALE C91382 5 FEMALE C91383 5 FEMALE C91384 5 FEMALE C91385 5 FEMALE C91386 5 FEMALE C91387 5 FEMALE C91388 5 FEMALE C91389 5 FEMALE C91390 5 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 4 SCHEDULED FOURTH INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 4 SCHEDULED FOURTH INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED DATE OF DAY OF DEATH STUDY 01/25/00 07/02/99 07/25/99 01/26/00 01/26/00 01/27/00 11/29/99 01/02/00 01/27/99 09/17/99 01/27/99 01/27/99 06/21/99 04/29/98 01/27/99 01/26/00 05/17/99 12/06/99 08/27/99 01/25/00 06/06/99 01/27/00 04/29/98 01/25/00 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 730 523 546 731 731 732 673 707 367 600 367 367 512 94 367 731 477 680 579 730 497 732 94 730 54 54 54 54 54 54 54 54 54 54 WEEK OF STUDY 105 75 78 105 105 105 97 101 53 86 53 53 74 14 53 105 69 98 83 105 71 105 14 105 8 8 8 8 8 8 8 8 8 8 PAGE: 20 705 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91391 5 FEMALE C91392 5 FEMALE C91393 5 FEMALE C91394 5 FEMALE C91395 5 FEMALE C91396 5 FEMALE C91397 5 FEMALE C91398 5 FEMALE C91399 5 FEMALE C91400 5 FEMALE C91401 5 FEMALE C91402 5 FEMALE C91403 5 FEMALE C91404 5 FEMALE C91405 5 FEMALE C91406 5 FEMALE C91407 5 FEMALE C91408 5 FEMALE C91409 5 FEMALE C91410 5 FEMALE C91411 5 FEMALE C91412 5 FEMALE C91413 5 FEMALE C91414 5 FEMALE C91415 5 FEMALE C91416 5 FEMALE C91417 5 FEMALE C91418 5 FEMALE C91419 5 FEMALE C91420 5 FEMALE C91421 5 FEMALE C91422 5 FEMALE C91423 5 FEMALE C91424 5 FEMALE DEATH CODE 6 6 2 2 6 6 6 6 6 6 6 6 6 2 6 6 6 6 6 6 6 6 6 6 2 6 6 6 6 6 6 6 1 6 TYPE OF DEATH SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SECOND INTERIM SACRIFICE SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED FIRST INTERIM SACRIFICE SACRIFICED AND DISCARDED DATE OF DAY OF DEATH STUDY 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 54 24 54 WEEK OF STUDY 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 4 8 PAGE: 21 706 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91425 5 FEMALE C91426 5 FEMALE C91427 5 FEMALE C91428 5 FEMALE C91429 5 FEMALE C91430 5 FEMALE C91431 5 FEMALE C91432 5 FEMALE C91433 5 FEMALE C91434 5 FEMALE C91435 5 FEMALE C91436 5 FEMALE C91437 5 FEMALE C91438 5 FEMALE C91439 5 FEMALE C91440 5 FEMALE C91441 5 FEMALE C91442 5 FEMALE C91443 5 FEMALE C91444 5 FEMALE C91445 5 FEMALE C91446 5 FEMALE C91447 5 FEMALE C91448 5 FEMALE C91449 5 FEMALE C91450 5 FEMALE C91451 6 FEMALE C91452 6 FEMALE C91453 6 FEMALE C91454 6 FEMALE C91455 6 FEMALE C91456 6 FEMALE C91457 6 FEMALE C91458 6 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION <OF DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 1 SCHEDULED FIRST INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 1 SCHEDULED FIRST INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 2 SCHEDULED SECOND INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 1 SCHEDULED FIRST INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED 1 SCHEDULED FIRST INTERIM SACRIFICE 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST-RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 03/20/98 03/20/98 02/18/98 03/20/98 03/20/98 01/27/00 06/21/99 01/27/00 08/26/99 01/27/00 07/12/99 11/23/99 04/05/99 54 54 24 54 54 24 54 54 54 54 54 54 54 54 54 54 54 54 24 54 54 54 54 24 54 54 732 512 732 578 732 533 667 435 WEEK OF STUDY 8 8 4 8 8 4 8 8 8 8 8 8 8 8 8 8 8 8 4 8 8 8 8 4 8 8 105 74 105 83 105 77 96 63 PAGE: 22 707 APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91459 6 FEMALE C91460 6 FEMALE C91461 6 FEMALE C91462 6 FEMALE C91463 6 FEMALE C91464 6 FEMALE C91465 6 FEMALE C91466 6 FEMALE C91467 6 FEMALE C91468 6 FEMALE C91469 6 FEMALE C91470 6 FEMALE C91471 6 FEMALE C91472 6 FEMALE C91473 6 FEMALE C91474 6 FEMALE C91475 6 FEMALE C91476 6 FEMALE C91477 6 FEMALE C91478 6 FEMALE C91479 6 FEMALE C91480 6 FEMALE C91481 6 FEMALE C91482 6 FEMALE C91483 6 FEMALE C91484 6 FEMALE C91485 6 FEMALE C91486 6 FEMALE C91487 6 FEMALE C91488 6 FEMALE C91489 6 FEMALE C91490 6 FEMALE C91491 7 FEMALE C91492 7 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH U SCHEDULED FIRST POST- RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC U SCHEDULED FIRST POST- RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC M UNSCHEDULED UNSCHEDULED DEATH U SCHEDULED FIRST POST- RECOV SAC U SCHEDULED FIRST POST- RECOV SAC D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 6 SCHEDULED SACRIFICED AND DISCARDED 6 SCHEDULED SACRIFICED AND DISCARDED DATE OF DAY OF DEATH STUDY 01/27/00 03/30/99 09/17/99 01/27/00 09/25/99 06/21/99 10/11/99 11/03/99 01/27/00 04/12/99 06/09/99 05/17/99 12/01/99 12/06/99 01/27/00 11/01/99 12/23/99 08/05/99 09/13/99 09/10/99 01/27/00 01/27/00 06/18/99 11/01/99 11/22/99 05/31/99 01/27/00 10/27/99 01/27/00 01/27/00 08/09/99 08/23/99 03/20/98 03/20/98 732 429 600 732 608 512 624 647 732 442 500 477 675 680 732 645 697 557 596 593 732 732 509 645 666 491 732 640 732 732 561 575 54 54 WEEK OF STUDY 105 62 86 105 87 74 90 93 105 64 72 69 97 98 105 93 100 80 86 85 105 105 73 93 96 71 105 92 105 105 81 83 8 8 PAGE: 23 708 Individual Animal Fate Data C91523 7 FEMALE 6 SCHEDULED SACRIFICED AND DISCARDED 03/20/98 C91524 7 FEMALE 6 SCHEDULED SACRIFICED AND DISCARDED 03/20/98 C91525 7 FEMALE 6 SCHEDULED SACRIFICED AND DISCARDED 03/20/98 C91526 7 FEMALE 6 SCHEDULED SACRIFICED AND DISCARDED 03/20/98 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M o a pH i i ain h Kj i i d d in i a 1-3 i Od iaD i > Kj i2 i PH s! i D PH i OH ia ia i ha i i i aP a > a a od oa oooooooooooooooooooooooooooooo OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ to to to to to to to to to to to to to to to to to to to to to to to to to to to to to to oooooooooooooooooooooooooooooo <><><><><><><><><><><><><><><> CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO CO D Da > h >a Ha o a i i i i i i i a> Qa Hcn a oe cnD> H kJ dDkJO^ oeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoeoe cn pd H Pd d * D Kj o o o <> OJpo 2 (D 1-3CT CTIOtJO OJ P-1 to APPENDIX 2 Individual Animal Fate Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL DOSE NUMBER GROUP SEX C91527 7 FEMALE C91528 7 FEMALE C91529 7 FEMALE C91530 7 FEMALE DEATH CODE 6 6 6 6 TYPE OF DEATH SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED SACRIFICED AND DISCARDED DATE OF DAY OF DEATH STUDY 03/20/98 03/20/98 03/20/98 03/20/98 54 54 54 54 WEEK OF STUDY 8 8 8 8 PAGE: 25 710 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90711 M 79 APPEARANCE HUNCHED THIN BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT SQUINTED PERI-ORBITAL RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS 547 526, 533, 540, 547 547 526, 533, 540, 547 414, 449, 456 547 547 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 421, 428, 435, 442 , 463, 470, 477, 484 , 491 498, 505, 512, 519 711 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90712 T 105 APPEARANCE HUNCHED MALOCCLUSION THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NO FECES NONFORMED FECES SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS 727, 729, 731 92, 99, 106, 113, 120, 127, 176, 183, 190, 197, 204, 211, 218 225, 232 , 239 , 246 , 253 , 260 , 267 , 274 , 281 , 288 , 295 , 302 , 309 316, 323 , 330 , 337 , 344 , 351 , 358 , 365 , 372 , 379 , 386 , 393 , 400 407, 414 , 421 , 428 , 435 , 442 , 449 , 456 , 463 , 470 , 477 , 484 , 491 498, 505 , 512 , 519 , 526 , 533 , 540 , 547 , 554 , 561 , 568 , 575 , 582 589, 596 , 603 , 610 , 617 , 624 , 631 , 638 , 645 , 652 , 659 , 666 , 673 680, 687 , 694 , 701 , 708 , 715 , 722 , 727 , 729 , 731 727, 729 , 731 729, 731 729, 731 727 113, 358, 365, 372 729, 731 1, 8,, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 134 155, 162, 169 712 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90713 M 98 APPEARANCE THIN BEHAVIOR RECUMBENT LATERAL EXCRETION NONFORMED FECES EYES PALE EYE-RIGHT RESPIRATION LABORED SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB AXILLARY REGION-RIGHT TAIL NORMAL NO REMARKABLE OBSERVATIONS 666, 673, 680, 684 684 113, 155, 218 680, 684 680, 684 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652 659, 666, 673, 680, 684 267, 274 281 1, 8, 15 120, 127 225, 232 337, 344 428, 435 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 134, 141 , 148, 162, 169, 176 , 183, 190, 197, 204 , 211 239, 246 , 253, 260, 288, 295 , 302, 309, 316, 323 , 330 351, 358 , 365, 372, 379, 386 , 393, 400, 407, 414 , 421 442, 449 , 456, 463, 470, 477 , 484 713 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90713 M 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 C90714 4 53 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C90715 M 93 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NO FECES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C 113, 218 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 120, 127, 134, 141, 148, 155, 162, 169 , 176, 183, 190, 197, 204 211, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358 , 365, 367 631, 638, 645, 648-649 648-649 649 648 649 649 649 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 714 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90715 M 93 NORMAL NO REMARKABLE OBSERVATIONS T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL WEEK 105 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624 603, 610, 617, 624, 631, 638, 645, 652 , 659, 666, 673, 680 , 687 694, 701, 708, 715, 722, 729- 730 526, 533, 540, 547, 554, 561, 568, 575 , 582 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 589 , 596 729-730 715 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90717 2 C90718 2 C90719 1 C90720 D 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 89 NORMAL NO REMARKABLE OBSERVATIONS C90721 D 44 APPEARANCE SWOLLEN LIMB-HIND-LEFT BEHAVIOR RECUMBENT STERNAL EXCRETION FEW FECES NO FECES 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8,r 15, 22, 24 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 -618 64, 71, 78, 85 302 302, 305 281 716 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90721 D 44 EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA PALE ENTIRE BODY SORE/SCAB LATERAL-CERVICAL-LEFT LATERAL-CERVICAL-RIGHT VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 43 MOUTH WEEK 20 VENTRAL-THORACIC WEEK 42 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 281, 288 302, 305 302, 305 8, 15, 22, 29 8, 15, 22, 29 281 1, 36, 43, 50 57, 92, 99, 106, 113, 120, 127, 155, 162, 169 176 , 183, 190 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267 , 274 295C 134, 141, 148 288, 295C, 302C, 305C 717 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 8 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90722 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90723 M 61 APPEARANCE PROTRUDING PENIS NORMAL NO REMARKABLE OBSERVATIONS C90724 T SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414 603, 610, 617,. 624, 631, 638, 645, 652 , 659, 666, 673, 680, 687 694, 701, 708,. 715, 722, 729- 730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561 718 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90724 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90725 1 4 NORMAL NO REMARKABLE OBSERVATIONS C90726 T 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB DORSAL PAW-HIND-RIGHT PAWS-HIND YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL WEEK 51 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568, 575, 582, 589, 596 1, 8, 15, 22, 24 526, 547, 554, 561, 568 204, 211, 218, 225 , 232, 239 701, 708, 715, 722 , 729, 731 526, 533, 540, 547 , 554, 561, 568, 575 , 582, 589, 596, 603 , 610 617, 624, 631, 638 , 645, 652, 659, 666 , 673, 680, 687, 694 603, 610, 617, 624 , 631, 638, 645, 652 , 659, 666 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 246, 253, 260, 267 , 274, 281, 288, 295 , 302, 309, 316, 323 , 330 337, 344 351, 358, 365, 372 , 379, 386, 393, 400 , 407, 414, 421, 428 , 435 442, 449, 456, 463 , 470, 477, 484, 491 , 498, 505, 512, 519 , 526 533, 540, 547, 554 , 561, 568 719 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90727 M 97 APPEARANCE THIN EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) PAW-FRONT-LEFT WEEK 95 PAW-FRONT-RIGHT WEEK 95 T 105 EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL 666, 673 673 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673 659, 666, 673 596 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673 720 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 11 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90728 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90729 D 93 NORMAL NO REMARKABLE OBSERVATIONS C90730 M 69 APPEARANCE HEAD TILT RIGHT HUNCHED THIN SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 680, 687, 694, 701, 708, 715, 722, 729 -730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 477, 480 477, 480 477, 480 477, 480 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 721 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 12 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90731 3 C90732 1 C90733 D 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 96 APPEARANCE LIMITED USE PAW-HIND-RIGHT PAWS-HIND SWOLLEN PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) EAR-LEFT WEEK 93 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 1, 8, 15, 22, 24 414 526, 533, 540, 547, 554 393, 400 414, 421, 428, 582, 589, 596, 603, 610, 617, 624, 631, 638 526, 533, 540, 547, 554 526, 533, 540, 547, 554 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 407, 435, 442 , 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519 645 722 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 13 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90733 D 96 WART LIKE LESION(S) TAIL WEEK 82 C90734 M 80 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION NO FECES EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE COLD TO TOUCH ENTIRE BODY SORE/SCAB AXILLARY REGION-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 666 547, 554 554 547, 554 421, 547, 554 554 491, 498, 505 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414 723 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 14 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90734 M 80 MASS(ES) AXILLARY REGION-LEFT WEEK 61 C90735 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C90736 M 72 APPEARANCE HUNCHED BEHAVIOR HYPOACTIVE RECUMBENT LATERAL EXCRETION FEW FECES DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 421, 428, 435, 442, 449, 456 , 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526C , 533C', 54 0C, 547C, 554C 631, 638, 645, 652, 659, 666 , 673, 680 , 687, 694, 701, 708 , 715 722, 729- 730 547, 554, 561, 568, 575, 582 , 589, 596 , 603, 610, 617, 624 1, 8,r 15, 22, 29, 36 , 43, 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 , 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239 , 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330 , 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421 , 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512 , 519, 526 , 533, 540 502, 504 498, 502, 504 504 504 724 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 15 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90736 M 72 NORMAL NO REMARKABLE OBSERVATIONS C90737 2 C90738 D 8 NORMAL NO REMARKABLE OBSERVATIONS 99 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C90739 M 102 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 1, 8, 15, 22, 29, 36, 43, 50 54 358 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 365, 372, 379 , 386 393, 400, 407 414, 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498 505, 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589 596, 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673, 680 687, 689 713-714 713-714 713-714 725 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 16 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90739 M 102 EXCRETION NO FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL NORMAL NO REMARKABLE OBSERVATIONS C90740 M 66 BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE COLD TO TOUCH ENTIRE BODY DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 713-714 714 713-714 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 456 456 456 456 726 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 17 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90740 M 66 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C90741 M 100 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NONFORMED FECES SKIN & PELAGE SORE/SCAB PAWS-HIND VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 351, 414, 421,, 428, 435, 442, 449, 456 1, 8,r 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 358, 365, 372, 379 , 386 393, 400, 407 680, 687- 688, 691-692, 694, 696 696 680, 687- 688, 691-692, 694, 696 575 680, 687- 688, 691-692, 694, 696 673 680, 687- 688, 691-692, 694, 696 1, 8,r 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 727 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 18 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90741 M 100 NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) VENTRAL-THORACIC-RIGHT WEEK 69 C90742 T 105 NORMAL NO REMARKABLE OBSERVATIONS T APPEARANCE LIMITED USE LIMB-HIND-RIGHT SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428 , 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519 , 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610 , 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687- 688, 691- 692, 694, 696 1, 8,r 15, 22, 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155 , 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246 , 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337 , 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428 , 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519 , 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610 , 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701 , 708 , 715, 722, 729- 730 491, 498, 505, 512 , 519, 526, 533,. 540 , 547, 554, 561, 568 , 575 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 582, 589 , 596, 603, 610, 617 , 624 728 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 19 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90743 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90744 M MASS(ES) DORSAL WEEK 49 WART LIKE LESION(S) DORSAL WEEK 61 87 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION NO FECES EYES SQUINTED PERI-ORBITAL DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 708, 715, 722 729, 731 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 603-604 603-604 603-604 603-604 600, 603-604 603-604 729 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 20 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90744 M 87 SKIN & PELAGE RED HAIR COAT LIMBS-FRONT NORMAL NO REMARKABLE OBSERVATIONS C90745 M 77 APPEARANCE HUNCHED MALOCCLUSION THIN OTHER BEHAVIOR SENSITIVE TO TOUCH EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 603-604 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596 533 92, 99, 106, 113, 120, 127, 141, 148, 155, 162, 169, 176, 183 190, 197 , 204 , 211 , 218 , 225 , 232 , 239 , 246 , 253 , 260 , 295 , 3 02 309, 316 , 323 , 330 , 337 , 344 , 351 , 358 , 365 , 372 , 379 , 386 , 3 93 400, 407 , 414 , 421 , 428 , 435 , 442 , 449 , 456 , 463 , 470 , 477 , 4 84 491, 498 , 505 , 512 , 519 -520, 526, 533 526, 533 520C 533 526, 533 526, 533 730 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 21 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90745 M 77 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C90746 3 C90747 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE HUNCHED THIN EXCRETION FEW FECES NONFORMED FECES EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB TAIL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519-520, 526, 533 1, 8, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78 , 85, 134, 267, 274 281, 288 1, 8, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78 , 85, 92, 94 729730 547, 554, 561,, 568, 575, 582, 589, 596 , 603, 610, 617,. 624, 680 687, 694, 701,, 708, 715, 722, 729-730 547, 554, 561 575 729- 730 526, 533, 540,, 547, 554, 561, 568 477, 484 1, 8, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78 , 85, 92, 99, 106 731 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 22 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90747 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90748 3 C90749 M MASS(ES) INGUINAL REGION-RIGHT WEEK 97 14 NORMAL NO REMARKABLE OBSERVATIONS 93 APPEARANCE PROTRUDING PENIS THIN BEHAVIOR HYPOACTIVE RECUMBENT STERNAL EXCRETION NO FECES EYES PALE EYES RED DISCHARGE EYES DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 491, 498, 505, 512, 519, 631, 638, 645, 652, 659, 666 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C , 729- 730C 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64 , 71, 78, 85, 92 , 94 645-646 645-647 647 647 646-647 647 647 732 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 23 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90749 M 93 RESPIRATION LABORED SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 647 647 645-647 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638 575, 582, 589,. 596, 603, 610, 617, 624 , 631 638, 645, 652, 659, 666, 673, 680, 687 , 694, 701, 708, 715 , 722 729, 731 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 5c6tz8o 733 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 24 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90750 T 105 WART LIKE LESION(S) DORSAL WEEK 105 C90751 4 53 NORMAL NO REMARKABLE OBSERVATIONS C90752 4 53 EYES CLEAR DISCHARGE EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS C90753 1 4 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 729, 731 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 22, 29 15 1, 8,r 36, 43, 50, 57, 64,r 71, 78, 85, 92, 99, 106,r 113,, 120, 127 134, 141, 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 211, 218 225, 232, 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302, 309 316, 323, 330, 337 , 344, 351, 358, 365 , 367 1, 8, 15, 22, 24 734 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 25 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90754 4 53 NORMAL NO REMARKABLE OBSERVATIONS D 100 EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 80 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 367 533 526, 533, 540, 547, 554, 561, 568, 575 , 582, 589, 596, 603 , 610 617, 624, 631,. 638, 645, 652, 659, 666 , 673, 680, 687, 694 -695 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519 554, 561, 568 735 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 26 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90756 2 C90757 M 8 NORMAL NO REMARKABLE OBSERVATIONS 70 APPEARANCE SWOLLEN DORSAL-CERVICAL LIMB-HIND-LEFT BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES RESPIRATION AUDIBLE SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 484 488 488 477, 484, 488 488 488 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 736 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 27 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90758 T 105 APPEARANCE LIMITED USE LIMBS-HIND SWOLLEN LIMBS-HIND PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C90759 M 86 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 547, 554, 561 547, 554, 561 708, 715, 722, 729,, 731 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 568, 575 , 582 589, 596, 603,. 610, 617, 624, 631, 638 , 645, 652, 659, 666 , 673 680, 687, 694, 701 554, 561, 568, 575, 582, 589, 596-597 547, 554, 561, 568, 575, 582, 589, 596-597 554, 561, 568, 575, 582, 589, 596-597 561, 568, 575, 582, 589, 596-597 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 737 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 28 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90759 M 86 NORMAL NO REMARKABLE OBSERVATIONS C90760 D 85 APPEARANCE SWOLLEN VENTRAL-CERVICAL BEHAVIOR SENSITIVE TO TOUCH DISCHARGE GENITAL RED IN COLOR EXCRETION DISCOLORED URINE RED IN COLOR ORANGE IN COLOR EYES CLEAR DISCHARGE EYE-LEFT DISCOLORED/LIGHT RED EYE-LEFT RED DISCHARGE EYE-LEFT EYES DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 568 232, 239, 246, 309 309 232 246 533, 540 547 554, 561 568, 575, 582, 589, 593 281, 288 295, 302, 337, 344, 351, 358, 365, 372, 379, 386 393 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 554 477, 484 491, 498, 505, 512, 519 738 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 29 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER (CONTINUED FROM PREVIOUS PAGE) C90760 D 85 SKIN & PELAGE SORE/SCAB LIMBS-HIND PAWS-HIND GROUP: Ml YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C90761 D 95 NORMAL NO REMARKABLE OBSERVATIONS D 79 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 337, 344 351, 358, 365, 372, 379, 386, 393, 400 , 407, 414, 421, 428 , 435 442, 449, 456, 463, 470, 477, 484, 491 , 498, 505, 512, 519 , 526 533, 540, 547, 554, 561, 568, 575, 582 , 589, 593 568, 575, 582, 589, 593 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 253, 260, 267, 274 , 316, 323, 330 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 662 512, 519, 526 533, 540, 547- 548 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 739 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 30 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90762 D 79 NORMAL NO REMARKABLE OBSERVATIONS C90763 M 87 APPEARANCE LIMITED USE LIMBS-FRONT LIMBS-HIND BEHAVIOR ATAXIC DISCHARGE ORAL RED IN COLOR EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505 603-604 603-604 603-604 589 596, 603-604 603-604 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 740 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 31 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90763 M 87 NORMAL NO REMARKABLE OBSERVATIONS C90764 D 84 EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS C90765 T MASS(ES) AXILLARY REGION-RIGHT WEEK 84 105 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568, 575, 582 575 491, 498, 505 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 512 519, 526, 533, 540, 547 , 554, 561, 568 582, 587 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 701, 708, 715, 722 , 729- 730 741 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 32 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90765 T 105 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 82 C90766 M 63 BEHAVIOR HYPOACTIVE DISCHARGE ORAL RED IN COLOR NASAL RED IN COLOR EYES RED DISCHARGE EYES RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE COLD TO TOUCH ENTIRE BODY ROUGH HAIR COAT SORE/SCAB DORSAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568, 575 440 440 440 440 440 440 440 440 440 22, 29, 36, 43, 50,. 57, 64, 71, 78 , 85, 92 1, 8, 15, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169 176, 183, 190, 197, 204,, 211, 218, 225,. 232, 239,. 246,, 253, 260 742 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 33 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90766 M 63 NORMAL NO REMARKABLE OBSERVATIONS C90767 4 53 NORMAL NO REMARKABLE OBSERVATIONS C90768 3 C90769 2 C90770 M 14 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 92 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NO FECES RESPIRATION LABORED DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 267, 274, 281,, 288, 295, 302, 309, 316 , 323, 330, 337, 344 , 351 358, 365, 372,, 379, 386, 393, 400, 407 , 414, 421, 428, 435 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365, 367 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 94 1, 8,, 15, 22, 29, 36, 43,, 50, 54 629 631, 638, 640 638, 640 629, 631 638, 640 640 743 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 34 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90770 M 92 SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C90771 M 94 BEHAVIOR HYPOACTIVE RECUMBENT LATERAL DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 603, 610, 617, 624 631, 638, 640 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596 652-653, 655 655 652-653, 655 653, 655 652-653, 655 744 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 35 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90771 M 94 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C90772 M MASS(ES) AXILLARY REGION-RIGHT WEEK 84 62 APPEARANCE LIMITED USE LIMBS-HIND EYES RED DISCHARGE EYES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 589, 596, 603, 610 , 617, 624 603, 610, 617, 624 , 631, 638, 645, 652 -653, 655 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575 582 422, 428 428 428 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 745 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 36 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90772 M 62 NORMAL NO REMARKABLE OBSERVATIONS C90773 D 65 NORMAL NO REMARKABLE OBSERVATIONS C90774 M 72 APPEARANCE MALOCCLUSION MISSING TEETH THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES RESPIRATION LABORED SKIN & PELAGE RED HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 455 15, 22, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260 141,, 148, 155, 162, 169 491, 498, 500 500 477, 484, 489, 491, 498, 500 500 477, 484, 489, 491, 498, 500 477, 484, 489, 491, 498, 500 746 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 37 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90774 M 72 SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C90775 1 C90776 D 4 NORMAL NO REMARKABLE OBSERVATIONS 100 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 489, 491 , 498, 500 1, 8, 29, 36, 43, 50, 57,, 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 176, 267 , 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344, 351, 358 , 365, 372, 379,. 386, 393, 400, 407, 414, 421 428, 435, 442, 449 , 456, 463, 470 1, 8,, 15, 22, 24 211, 344 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505, 512 , 519, 526, 533, 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 696 747 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 38 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90777 M 88 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION NO FECES EYES PALE EYES SQUINTED PERI-ORBITAL HOUSING,FOOD,WATER & MAINT. WATER BOTTLE PLACED ON CAGE SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-THORACIC SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 603, 609-611 603, 609-612 600, 603, 609-612 603, 609-612 603, 609-612 600 603, 609 612 603, 609 612 540, 547 554, 561, 568, 575, 582, 589 , 596, 600, 603, 609 -612 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533 748 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 39 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90778 T 105 EYES OPAQUE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 C90779 M 93 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NO FECES EYES OPAQUE EYE-RIGHT 729, 732 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568C, 575C , 582C, 589C, 596C, 603C , 610C, 617C, 624C, 631C , 638C 645C, 652C , 659C, 666C, 673C, 680C , 687C, 694C, 701C, 708C , 715C 722C,. 72 9C , 732C 641, 645, 647 647 641, 645 647 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 641, 645, 647 749 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 40 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: Ml (CONTINUED FROM PREVIOUS PAGE) C90779 M 93 EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C90780 3 14 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 647 647 647 641, 645 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 94 750 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1% N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 71 MASS(ES) 83 AXILLARY REGION-RIGHT WEEK 71 43 MASS(ES) AXILLARY REGION-LEFT WEEK 43 43 MASS(ES) VENTRAL-THORACIC WEEK 42 44 MASS(ES) 44 VENTRAL-THORACIC WEEK 42 76 MASS(ES) AXILLARY REGION-LEFT WEEK 61 77 MASS(ES) 80 AXILLARY REGION-LEFT WEEK 61 75 APPEARANCE OTHER 97 MASS(ES) 98 INGUINAL REGION-RIGHT WEEK 97 10 X 10MM 30 X 30MM 30 X 50MM 30 X 30MM 15 X 15MM 20X20 MM EXCESSIVE BLEEDING FROM PAWHR, CAUTERIZED WITH 25 X 50MM 751 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 99 MASS(ES) 100 INGUINAL REGION-RIGHT WEEK 97 101 MASS(ES) INGUINAL REGION-RIGHT WEEK 97 102 MASS(ES) 104 INGUINAL REGION-RIGHT WEEK 97 105 MASS(ES) 105 INGUINAL REGION-RIGHT WEEK 97 82 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 83 MASS(ES) 92 VENTRAL-ABDOMINAL-RIGHT WEEK 82 93 MASS(ES) 94 VENTRAL-ABDOMINAL-RIGHT WEEK 82 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 50 X 30MM 60 X 45MM 70 X 45MM 80 X 60MM 25MM X 20MM 30MM X 25MM 40 X 40MM 55 X 50MM 752 Covance 6329-212 3M T-6316.1 PAGE: 42 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C90778 M1 666 96 MASS(ES) TO 694 100 VENTRAL-ABDOMINAL-RIGHT WEEK 82 701 101 MASS(ES) TO 722 104 VENTRAL-ABDOMINAL-RIGHT WEEK 82 729 105 MASS(ES) TO 732 105 VENTRAL-ABDOMINAL-RIGHT WEEK 82 60 X 55MM 80 X 75MM 90 X 80MM Covance 6329-212 3M T-6316.1 PAGE: 43 753 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 44 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90781 T 105 SKIN & PELAGE SORE/SCAB TAIL VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS C90782 T WART LIKE LESION(S) VENTRAL-THORACIC-RIGHT WEEK 87 APPEARANCE SWOLLEN PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 547, 554, 561, 568 , 575, 603 , 610, 617, 624, 631 , 638 645, 652, 659, 666, 673, 680 , 687, 694 15, 22, 29 , 36 , 43, 50, 57, 64, 71 , 78 , 85, 92 1, 8, 99, 106, 113, 120, 127 , 134, 141 , 148, 155, 162, 169 , 176 183, 190, 197, 204, 211, 218 , 225, 232 , 239, 246, 253, 260 , 267 274, 281, 288, 295, 302, 309 , 316, 323 , 330, 337, 344, 351 , 358 365, 372, 379, 386, 393, 400 , 407, 414 , 421, 428, 435, 442 , 449 456, 463, 470, 491, 498, 505 , 512, 519 , 526, 533, 540, 582 , 589 596, 701, 708, 715, 722, 729 -730 603, 610, 617, 624 666, 673, 680, 687, 694, 701 , 708, 715 , 722, 729, 731 351, 358, 365, 372, 379, 386 , 393, 400 , 407, 414, 421, 428 , 435 442, 449, 456, 463, 470, 477 , 484, 652 , 659, 666, 673, 680 , 687 694, 701, 708, 715, 722, 729 , 731 526, 533, 540, 547, 554, 561 , 568, 575 , 582, 589, 596, 603 , 610 617, 624, 631, 638, 645 1, 8, 15, 22, 29, 36 , 43 , 50 , 57, 64, 71, 78 , 85, 92, 99, 106 754 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 45 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90782 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90783 T MASS(ES) DORSAL WEEK 105 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C90784 M 94 APPEARANCE PARALYSIS LIMBS-HIND THIN BEHAVIOR HYPOACTIVE RECUMBENT LATERAL DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 491, 498, 505, 512, 519 729, 731 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715 722, 729, 731 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505, 512, 519 651 645, 651-652 651-652 652 755 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 46 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90784 M 94 BEHAVIOR RECUMBENT STERNAL EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT VENTRAL-THORACIC NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE HUNCHED THIN EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 652 652 526, 533,. 540, 547, 554, 561, 568, 575 , 582, 589, 596, 603 , 610 617, 624, 631,. 638, 645, 651- 652 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 651 -652 1, 8, 15,. 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211,. 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519 728- 729, 731 728- 729, 731 728- 729, 731 728- 729, 731 756 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 47 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90785 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90786 D MASS(ES) AXILLARY REGION-LEFT WEEK 89 WART LIKE LESION(S) TAIL WEEK 104 93 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582,. 589, 596, 603, 610 617C, 624C , 631C, 638C, 645C, 652C , 659C , 666C, 673C, 680C , 687C 694C, 701C , 708C, 715C, 722C, 728- 729C, 731C 728-729, 731 316 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498 505 , 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589 596 , 603, 610, 617, 624 , 631, 638, 645- 646 757 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 48 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90787 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB SHOULDER-LEFT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 69 T 105 SKIN & PELAGE SORE/SCAB HEAD-CRANIAL LIMB-HIND-LEFT PAWS-HIND SHOULDER-LEFT NORMAL NO REMARKABLE OBSERVATIONS 694, 701, 708, 715, 722, 729, 731 92, 99, 106, 113, 120, 127 1, 8, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 134, 141, 148 155, 162, 169,. 176, 183, 190, 197, 204 , 211, 218, 225, 232, 239 246, 253, 260, 267, 274, 281, 288, 295 , 302, 309, 316, 323, 330 337, 344, 351,. 358, 365, 372, 379, 386 , 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 631 , 638, 645, 652, 659, 666 673, 680, 687 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617 , 624 15 701 526, 533, 540, 547, 554, 561, 568, 575 , 582, 589, 596, 603, 610 617, 624, 631,. 638, 645, 652, 659, 666 , 673, 680, 687, 694 15, 22 1, 8, 29, 36, 43, 50, 57,. 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141,, 148, 155, 162, 169, 176 , 183, 190, 197, 204, 211 758 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 49 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90788 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90789 1 C90790 M 4 NORMAL NO REMARKABLE OBSERVATIONS 81 BEHAVIOR HYPOACTIVE RECUMBENT STERNAL EXCRETION FEW FECES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 708, 715, 722, 729, 732 1, 8, 15, 22, 24 561 561 561 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 , 533, 540, 547, 554 759 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 50 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90791 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90792 M 57 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION NO FECES EYES SQUINTED PERI-ORBITAL RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 393, 396 393, 396 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386 760 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 51 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90793 D 78 NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE LIMBS-ALL ROUGH HAIR COAT SORE/SCAB PAW-HIND-RIGHT 1, 8 , 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 239, 246 225, 232, 239 239 225, 232, 239 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687, 694 , 701, 708, 715, 722 , 729, 731 225, 232, 239 239 225, 232, 239 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 722, 729, 731 761 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 52 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90794 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90795 M WART LIKE LESION(S) PAW-HIND-RIGHT WEEK 34 58 APPEARANCE THIN DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES RESPIRATION AUDIBLE SKIN & PELAGE BROWN HAIR COAT GENERALIZED ROUGH HAIR COAT DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 253 , 260, 267, 274, 281 , 288, 295, 302, 309 , 316 323, 330, 337, 344 , 351, 358, 365, 372 , 379, 386, 393, 400 , 407 414, 421, 428, 435 , 442, 449, 456, 463 , 470 232, 239, 246 393, 400 400 379, 386, 393, 400 400 400 400 400 762 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 53 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90795 M 58 NORMAL NO REMARKABLE OBSERVATIONS C90796 D 59 APPEARANCE MALOCCLUSION EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) EAR-LEFT WEEK 47 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155,. 162, 169, 176, 183,, 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337,. 344, 351, 358, 365, 372 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211, 218, 225, 232, 239 407, 412 344 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 246, 253 260, 267, 274, 281, 288, 295, 302,. 309, 316, 386, 393, 400 323, 330, 337, 344, 351, 358, 365,. 372, 379 763 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 54 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90797 3 M 14 NORMAL NO REMARKABLE OBSERVATIONS 101 APPEARANCE MALOCCLUSION C90799 T EXCRETION FEW FECES NONFORMED FECES RESPIRATION AUDIBLE LABORED NORMAL NO REMARKABLE OBSERVATIONS 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 , 43 , 50 , 57, 64, 71, 78 , 85, 92, 94 29, 36, 43 , 50 , 57, 64, 71, 78, 85 , 92 , 99, 106, 113, 120, 127 134, 141, 148, 155, 162, 169 , 176, 183 , 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260 , 267, 274 , 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351 , 358, 365 , 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442 , 449, 456 , 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533 , 540, 547 , 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624 , 631, 638 , 645, 652, 659, 666, 673 680, 687, 694, 701, 703 703 533, 540 701, 703 701, 703 1, 8, 15, 22 708, 715 722, 729-730 1, 8, 15 113, 120 204, 211 295, 302 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 764 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 55 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90799 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90800 D WART LIKE LESION(S) MOUTH WEEK 87 83 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS D 98 APPEARANCE SWOLLEN LIMB-HIND-RIGHT PAWS-HIND EXCRETION NONFORMED FECES DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701 603, 610, 617, 624 575, 578 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568 323, 330, 337 509, 512, 519, 526 , 533, 540, 547, 554 211 765 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 56 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90801 D 98 SKIN & PELAGE SORE/SCAB LIMB-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C90802 M MASS(ES) LIMB-HIND-RIGHT WEEK 98 WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 76 81 APPEARANCE SWOLLEN PAW-HIND-LEFT THIN BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 323 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 344 , 351, 358, 365, 372 , 379, 386, 393, 400 , 407 414, 421, 428, 435 , 442, 449, 456, 463 , 470, 477, 484, 491 , 498 505, 561, 568, 575 , 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 659, 666 , 673 680C, 685C 526, 533, 540 540 554, 561-562 562 554, 561-562 766 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 57 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90802 M 81 RESPIRATION LABORED SKIN & PELAGE COLD TO TOUCH ENTIRE BODY RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C90803 D 93 EYES OPAQUE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 562 562 554, 561-562 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 547 617, 624, 631,. 638, 645, 651 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610 767 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 58 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90803 D 93 WART LIKE LESION(S) TAIL WEEK 93 C90804 T 105 APPEARANCE HUNCHED THIN EXCRETION FEW FECES EYES OPAQUE EYES SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 645, 651 729, 731 729, 731 728-729, 731 728-729, 731 701, 708, 715,. 722, 728- 729, 731 1, 8,r 15, 22, 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694 768 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 59 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90805 M 61 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE DISCHARGE GENITAL RED IN COLOR EXCRETION FEW FECES NONFORMED FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT SCROTUM RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 421, 423 421, 423 423 386, 393 400, 407, 414, 421, 423 211, 379, 386 393, 400, 407, 414, 421, 423 421, 423 407, 414 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288, 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372 769 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 60 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90806 M C90807 3 C90808 M 35 APPEARANCE THIN SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 14 NORMAL NO REMARKABLE OBSERVATIONS 71 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE RECUMBENT LATERAL EXCRETION FEW FECES 239 239 232, 239 1, 8,, 15, 22, 29, 36, 43,. 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 141, 148, 155, 162, 169, 176, 183C, 190C, 197C, 204C, 211C, 218C 225C, 232C , 239C 1, 8,, 15, 22, 29, 36, 43,. 50, 57, 64, 71, 78, 85, 92, 94 477, 484, 491 463, 470, 477,. 484, 491 477, 484, 491 491 477, 484 770 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 61 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90808 M 71 EXCRETION NO FECES RESPIRATION IRREGULAR LABORED SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C90809 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 491 477, 484 491 477, 484, 491 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456 729, 732 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 722 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 771 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 62 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90809 T 105 NORMAL NO REMARKABLE OBSERVATIONS SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB LIMB-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS T DISCHARGE GENITAL RED IN COLOR EXCRETION FEW FECES RESPIRATION AUDIBLE LABORED SKIN & PELAGE SORE/SCAB DORSAL DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 533, 540, 547,. 554, 561, 568, 575, 582 , 589, 596, 603, 610 , 617 624, 631, 638, 645, 652, 659, 666, 673 , 680, 687, 694, 701 568, 575, 582, 589 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 708, 715, 722, 729 , 731 323, 330, 337 323 323 680, 687, 694, 701, 708, 715, 722 772 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 63 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90811 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90812 T WART LIKE LESION(S) DORSAL WEEK 97 105 APPEARANCE HUNCHED THIN DISCHARGE NASAL RED IN COLOR SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519 , 526, 533, 540, 547 , 554, 561, 568, 575 , 582 589, 596, 603, 610 , 617, 624, 631, 638 , 645, 652, 659, 666 n7o2n9-7n~3>0r\ 673 729-730 680, 687, 694, 701, 708, 715, 718, 722, 729-730 729-730 701, 708, 715, 718 , 722, 729730 687, 694, 701, 708 , 715, 718, 722, 729-730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155,. 162, 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246,. 253, 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337,. 344, 351, 358, 365, 372 , 379 773 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 64 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90812 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90813 M WART LIKE LESION(S) VENTRAL-ABDOMINAL-LEFT WEEK 79 8 APPEARANCE THIN DISCHARGE ORAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES RESPIRATION IRREGULAR SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673 547, 554, 561, 568, 575 52 50, 52 52 50, 52 52 50, 52 1, 8, 15, 22, 29, 36, 43 774 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 65 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90814 1 C90815 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL WEEK 76 C90816 M 86 APPEARANCE THIN BEHAVIOR HYPOACTIVE SENSITIVE TO TOUCH EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519 526, 533, 540, 547, 554, 561, 568, 575 , 582, 589, 596, 603 , 610 617, 624, 631,. 638, 645, 652, 659, 666 , 673, 680, 687, 694 , 701C 708C, 715C, 722C, 729-730C 582, 589, 596, 598 598 598 598 589, 596, 598 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 775 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 66 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90816 M 86 NORMAL NO REMARKABLE OBSERVATIONS C90817 1 C90818 3 C90819 D 4 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 92 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575 1, 8, 15, 22, 24 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 491 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 498 505, 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589 596, 603, 610, 617, 624 , 631, 638, 641 776 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 67 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90820 M 70 APPEARANCE HUNCHED BEHAVIOR HYPOACTIVE SENSITIVE TO TOUCH EXCRETION FEW FECES EYES RED DISCHARGE EYES RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS 485 485 485 485 485 485 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484 777 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 68 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90821 D 100 EYES DISCOLORED/LIGHT RED EYE-LEFT OPAQUE EYE-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS M 77 APPEARANCE THIN EXCRETION NO FECES SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL NORMAL NO REMARKABLE OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 659, 666, 673, 680, 687,. 694 -695 680, 687, 694- 695 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 526, 533 526, 533 533 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 778 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 69 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90822 M 77 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 76 T 105 APPEARANCE MALOCCLUSION EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB LIMB-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519 526C, 533C 680, 687, 694, 701 , 708, 715, 722, 729 -730 155, 344 631, 638, 645, 652 , 659, 666 1, 8,, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505, 512 , 519, 526, 533, 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603 , 610, 617, 624, 673 779 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 70 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90824 M 81 EXCRETION NO FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS D 98 NORMAL NO REMARKABLE OBSERVATIONS C90826 D 92 EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB TAIL 561 561 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 685 491, 575 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484 780 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 71 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90826 D 92 NORMAL NO REMARKABLE OBSERVATIONS M 68 BEHAVIOR HYPOACTIVE SENSITIVE TO TOUCH DISCHARGE ORAL CLEAR RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 498 , 505 512, 519, 526,. 533, 540, 547, 554, 561 , 568, 582, 589, 596 , 603 610, 617, 624, 631, 638, 642 471 471 471 471 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 781 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 72 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90828 D 80 EYES DILATED PUPIL EYES SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL WEEK 47 C90829 T 105 APPEARANCE OBESE EXCRETION NONFORMED FECES SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB AXILLARY REGION-RIGHT 547, 554, 560 491, 498, 505, 512 , 519, 526, 533, 540 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 323, 330, 337, 344 , 351, 358, 365, 372 , 379, 386, 393, 400 , 407 414, 421, 428, 435 , 442, 449, 456, 463 , 470, 477, 484 701, 708, 715, 722, 729 344 526, 533 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729 731 782 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 73 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90829 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 91 C90830 T WART LIKE LESION(S) VENTRAL-THORACIC-RIGHT WEEK 84 105 APPEARANCE PROTRUDING PENIS NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 351, 358, 365 , 372, 379 , 386 393, 400, 407,. 414, 421, 428, 435, 442, 449, 456 , 463, 470 , 477 484, 491, 498,. 505, 512, 519 631C, 638C , 645C, 652C, 659C, 666C , 673C, 680C, 687C, 694C , 701C 708C, 715C, 722C, 729C, 731C 582, 589, 596, 603, 610 617, 624 680, 687, 694 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673 701, 708, 715, 722, 729 , 731 783 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 74 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90831 3 C90832 T 14 NORMAL NO REMARKABLE OBSERVATIONS APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN NOSE DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES PALE EYES NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 94 225, 232, 239, 246 , 295, 323, 330, 337 , 344, 351, 358, 365, 372 379, 386, 393, 400 , 407, 414, 498, 505 , 512, 519, 526, 533,. 540 547, 554, 561, 568 , 575, 582, 589, 596 , 603, 609- 610, 617, 624 631, 638, 645, 652 , 659, 666, 673, 680 , 687, 694, 701, 708, 715 722, 729, 731 680, 687, 694, 701 , 708, 715, 722, 729 , 731 498, 505, 512, 519 , 526, 533, 540, 547 , 554, 561 505 505 505 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 253 , 260, 267, 274, 281 , 288, 302, 309,. 316, 421 428, 435, 442, 449 , 456, 463, 470, 477 , 484, 491 784 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 75 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90833 1 C90834 1 C90835 T 4 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 77 WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 85 1, 8, 15, 22, 24 1, 8, 15, 22, 24 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 547 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 582 533C, 540C 589, 596, 603, 610, 617, 624 785 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 76 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90836 3 C90837 M 14 NORMAL NO REMARKABLE OBSERVATIONS 74 APPEARANCE THIN BEHAVIOR SENSITIVE TO TOUCH EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 51 WEEK 61 WART LIKE LESION(S) TAIL WEEK 19 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 94 442, 449, 456, 463 , 470, 477, 484, 491 , 498, 505, 512 512 512 379, 512 1, 8, 15 113, 120 253, 260 344, 386 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 176, 183 , 190, 197, 204, 211 , 218, 225, 232, 239 , 246 267, 274 , 281, 288, 295, 302 , 309, 316, 323, 330 , 337 393, 400 , 407, 414 351, 358, 365, 372, 379 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C 127, 134, 141, 148, 155, 162, 169 786 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 77 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90838 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90839 D WART LIKE LESION(S) MOUTH WEEK 91 TAIL WEEK 22 99 APPEARANCE SWOLLEN LIMB-HIND-LEFT SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL BLUE SKIN PAW-HIND-LEFT SORE/SCAB VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 155, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589, 596 , 603, 610, 617, 624 631, 638, 645, 652 , 659, 666, 673, 680 , 687, 694, 701, 708 , 715 722, 729, 731 148 546- 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652 , 659, 666, 673, 680 , 687, 693 680, 687, 693 547, 554 680, 687, 693 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 787 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 78 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M2 (CONTINUED FROM PREVIOUS PAGE) C90839 D 99 NORMAL NO REMARKABLE OBSERVATIONS C90840 T 105 APPEARANCE SWOLLEN PAWS-HIND SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB LATERAL-ABDOMINAL-RIGHT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540 533, 540, 547, 554, 561, 568, 575, 582 , 589, 596, 603, 610 , 617 624, 631, 638, 645, 652, 659, 666, 673 , 680, 687, 694, 701 680, 687, 694, 701, 708, 715, 722, 729 -730 344, 351, 358, 365, 372 330, 337, 344, 351, 358 274, 281, 288 295, 302, 309, 316, 323, 365, 372, 379 , 386, 393, 400, 407 , 414 421, 428, 435, 442, 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526, 533, 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610, 617, 624, 631, 638, 645, 652 , 659, 666, 673, 680 , 687 694, 701, 708, 715, 722, 729- 730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267 351C, 358C, 365C, 372C, 379C 788 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C90785 C90801 C90806 M2 617 89 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 89 631 91 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 89 645 93 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 89 659 95 MASS(ES) TO 694 100 AXILLARY REGION-LEFT WEEK 89 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 89 708 102 MASS(ES) TO 731 105 AXILLARY REGION-LEFT WEEK 89 M2 680 98 TO 685 98 MASS(ES) LIMB-HIND-RIGHT WEEK 98 M2 183 27 TO 190 28 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 10 X 10MM 20 X 20MM 35 X 35MM 40 X 40MM 50 X 50MM 55 X 65MM 40 X 40MM 10 X 10MM 789 Covance 6329-212 3M T-6316.1 PAGE: 79 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C90806 C90815 C90822 M2 197 29 TO 204 30 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 211 31 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 218 32 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 225 33 TO 232 34 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 239 35 TO 239 35 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 239 35 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 21 M2 701 101 WART LIKE LESION(S) TO 730 105 DORSAL WEEK 76 M2 526 76 TO 533 77 MASS(ES) DORSAL WEEK 76 30 X 15MM 70 X 50MM 80 X 65MM 85 X 70MM 95 X 100MM 95 X 100MM 15 X 15MM 10 X 10MM 790 Covance 6329-212 3M T-6316.1 PAGE: 80 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C90829 C90835 C90837 M2 631 91 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 652 94 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 91 680 98 MASS(ES) TO 701 101 AXILLARY REGION-RIGHT WEEK 91 708 102 MASS(ES) TO 731 105 AXILLARY REGION-RIGHT WEEK 91 M2 533 77 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL WEEK 77 M2 421 61 TO 505 73 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 61 20 X 20MM 25 X 25MM 30 X 25MM 35 X 30MM 15 X 15MM 20 X 15MM 10 X 10MM 20 X 20MM 791 Covance 6329-212 3M T-6316.1 PAGE: 81 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C90837 C90840 M2 512 74 TO 512 74 512 74 M2 351 51 TO 379 55 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 61 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 20 X 25MM 20 X 20MM 10 X 10MM Covance 6329-212 3M T-6316.1 PAGE: 82 792 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 83 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90841 M 97 APPEARANCE HUNCHED THIN EXCRETION FEW FECES EYES DISCOLORED/LIGHT RED EYES RESPIRATION LABORED SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 677 677 673, 677 677 677 659, 666, 673, 677 309, 316, 323, 330, 337, 344, 351 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 582, 589, 596, 603, 610 673, 677 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 358, 365, 372, 379, 386, 393 , 400, 407, 414, 421 , 428 435, 442, 449,. 456, 463, 470 793 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 84 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90841 M 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 C90842 T 105 EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS 3 C90844 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-RIGHT SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SCALY SKIN TAIL SORE/SCAB TAIL DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575C 680, 687, 694 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 701, 708, 715, 722,. 729 , 731 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 94 708 725, 729, 731 526 526 568, 575 794 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 85 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90844 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90845 M WART LIKE LESION(S) TAIL WEEK 22 79 APPEARANCE THIN EXCRETION NO FECES SKIN & PELAGE BROWN HAIR COAT GENERALIZED BROWN SKIN PAWS-ALL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 155, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225,. 232, 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316,. 323, 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505, 512, 519, 533, 540 , 547, 554, 561, 582 , 589 596, 603, 610, 617, 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701,. 715, 722 148 547 547 547 547 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 795 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 86 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90845 M 79 NORMAL NO REMARKABLE OBSERVATIONS C90846 1 4 NORMAL NO REMARKABLE OBSERVATIONS C90847 M 99 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 1, 8, 15, 22, 24 680, 687 687 673, 680, 687 687 526, 533, 54 0, 547, 554, 561, 568 575, 582, 589,. 596, 603, 608, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673 687 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 796 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 87 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90847 M 99 NORMAL NO REMARKABLE OBSERVATIONS C90848 M 76 APPEARANCE MALOCCLUSION DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYES RESPIRATION AUDIBLE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 526 526 526 526 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519 797 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 88 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90849 M 78 APPEARANCE THIN BEHAVIOR SENSITIVE TO TOUCH EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 520, 526, 533, 540 512, 519-520, 526, 533, 540 520, 526, 533, 540 491, 498, 505,. 512, 519- 520, 526, 533, 540 1, 8,r 15, 22, 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484 638, 645, 652, 659, 666, 673, 680, 687 , 694, 701, 708, 715 , 722 729, 731 680, 687, 694, 701, 708, 715, 722, 729, 731 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 798 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 89 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90850 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90851 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS C90852 3 C90853 1 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729-730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 94 1, 8, 15, 22, 24 799 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 90 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90854 T 105 EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C90855 T 105 APPEARANCE PROTRUDING PENIS EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL-CERVICAL-LEFT WEEK 105 729, 731 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673 680, 687, 694, 701, 708 , 715, 722 680 281, 708, 715 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589, 596 , 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673, 687,. 694 , 701, 722 729, 732 800 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 91 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90856 T 105 NORMAL NO REMARKABLE OBSERVATIONS M 103 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 718- 719 680, 687, 694, 701, 708, 715, 718- 719 719 715, 718-719 715, 718-719 1, 8, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 801 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 92 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90857 M 103 NORMAL NO REMARKABLE OBSERVATIONS C90858 T 105 DISCHARGE NASAL RED IN COLOR SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS C90859 M 69 APPEARANCE THIN BEHAVIOR HYPOACTIVE RESPIRATION AUDIBLE SKIN & PELAGE YELLOW HAIR COAT DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659, 666, 673 589, 596 526, 533, 54 0, 547, 554, 561, 568, 575 , 582, 589, 596, 603 , 610 617, 624, 631,. 638, 645, 652, 659, 666 , 673 680, 687, 694, 701, 708, 715, 722 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 729 -730 463, 470, 477, 479 479 479 463, 470, 477, 479 802 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 93 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90859 M 69 NORMAL NO REMARKABLE OBSERVATIONS T 105 NORMAL NO REMARKABLE OBSERVATIONS C90861 M 71 BEHAVIOR HYPOACTIVE RECUMBENT STERNAL SENSITIVE TO TOUCH EXCRETION FEW FECES NO FECES SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL VENTRAL-ABDOMINAL ROUGH HAIR COAT DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 495 495 495 477, 484, 491 495 477, 484 495 477, 484 803 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 94 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90861 M 71 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS M 69 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC EYES SQUINTED PERI-ORBITAL RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 281, 288, 295 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 302 , 309 316, 323, 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470 477 477 477 477 477 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 804 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 95 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90863 3 C90864 T 14 NORMAL NO REMARKABLE OBSERVATIONS SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB SHOULDER-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 98 SHOULDER-LEFT WEEK 40 WART LIKE LESION(S) SHOULDER-LEFT WEEK 37 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 94 680, 687, 694, 701, 708, 715, 722,. 729 , 731 365, 372, 379 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 267 , 386, 393, 400, 407 , 414 421, 428, 435,. 442, 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526,. 533, 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610, 617,. 624, 631, 638, 645, 652 , 659, 666, 673 680, 687, 694, 701, 708, 715, 722,. 729 , 731 274, 281, 288, 295, 302, 309, 316, 323 , 330, 337, 344, 351 , 358 253, 260 805 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 96 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90865 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C90866 1 C90867 T 4 NORMAL NO REMARKABLE OBSERVATIONS 5 EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS 715, 722, 729, 732 1, 8,, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 1, 8,, 15, 22, 24 645, 652, 659 680, 687, 694, 701, 708, 715, 722,. 729 , 731 1, 8,, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 806 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 97 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER (CONTINUED FROM PREVIOUS PAGE) C90867 T 105 MASS(ES) DORSAL WEEK 64 GROUP: M3 C90868 M 73 APPEARANCE HUNCHED BEHAVIOR RECUMBENT LATERAL EXCRETION NO FECES RESPIRATION IRREGULAR SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL-LEFT SORE/SCAB HEAD-MAXILLARY-RIGHT LIMB-HIND-LEFT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND YELLOW HAIR COAT DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 442, 449, 456, 463 , 470, 477, 484, 491C, 498C, 505C, 512C, 519C 526C, 533C , 540C, 547C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C 603C, 610C , 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C , 694C, 701C, 708C, 715C, 722C, 72 9C, 731C 508 508 508 508 508 365, 372 323, 330, 337, 344 351, 358, 365, 372, 379 477, 484, 491, 498, 505 508 508 807 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 98 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90868 M 73 NORMAL NO REMARKABLE OBSERVATIONS C90869 T 105 APPEARANCE SWOLLEN PAWS-HIND SKIN & PELAGE SORE/SCAB SCROTUM SCROTUM-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 81 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 113, 120, 204, 211, 295, 302, 4a 4a 9n, 4A 5C 6tZ, 22, 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 386, 393, 4A 6fZ3, 4a 7n 0r\ 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 400, 407 , 414, 421, 92, 99, 106 183, 190 , 197 274, 281 , 288 428, 435 , 442 540, 547, 554, 561 694, 701 708,. 715, 722, 729- 730 568, 575 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 610, 617 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 582, 589, 596 , 603 624, 631, 638, 645, 652, 659 , 666, 673, 680, 687 561, 568, 575 808 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 99 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90870 M 80 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS T 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS 547, 554 554 547, 554 526, 533, 540, 547, 554 547, 554 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519 162, 169, 176, 183 , 211, 218 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 190 , 197, 204, 225, 232 , 239 246, 253, 260, 267 , 274, 281, 288, 295 , 302, 309, 316, 323 , 330 337, 344, 351, 358 , 365, 372, 379, 386 , 393, 400, 407, 414 , 421 428, 435, 442, 449 , 456, 463, 470, 477 , 484, 491, 498, 505 , 512 519, 526, 533,. 540 , 547, 554, 561, 568 , 575, 582, 589, 596 , 603 809 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 100 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90871 T 105 NORMAL NO REMARKABLE OBSERVATIONS D 91 EXCRETION FEW FECES NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C90873 M 73 APPEARANCE HEAD TILT RIGHT BEHAVIOR ATAXIC SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL VENTRAL-THORACIC SORE/SCAB PAW-HIND-LEFT DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 610, 617, 624, 631 , 638, 645, 652, 659 , 666, 673, 680, 687 , 694 701, 708, 715, 722 , 729- 730 631, 637 155 1, 8,r 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540, 547, 554, 561 , 568 575, 582, 589, 596 , 603, 610, 617, 624 507-508 507-508 507-508 507-508 470, 477, 484, 491, 498, 505, 507-508 810 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 101 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90873 M 73 NORMAL NO REMARKABLE OBSERVATIONS M 67 APPEARANCE THIN EXCRETION FEW FECES NONFORMED FECES EYES RED DISCHARGE EYES RESPIRATION AUDIBLE LABORED NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 463 393, 400, 407, 437, 442, 449, 456, 463 337 393, 463 393, 437, 463 393, 463 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 344, 351 , 358, 365, 372, 379, 386 414, 421, 428, 435 811 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 102 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90875 D 91 NORMAL NO REMARKABLE OBSERVATIONS C90876 A WART LIKE LESION(S) TAIL WEEK 22 56 APPEARANCE MALOCCLUSION C90877 3 PARALYSIS LIMBS-HIND NORMAL NO REMARKABLE OBSERVATIONS 14 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15 , 22, 29, 36, 43, 50 , 57, 64, 71, 78, 85 , 92, 99, 106 113, 120 , 127 , 134, 141, 218 , 225 , 232, 239, 246 , 253 , 260, 267 274, 281 , 288 , 295, 302, 309 , 316 , 323, 330, 337 , 344 , 351, 358 365, 372 , 379 , 386, 393, 400 , 407 , 414, 421, 428 , 435 , 442, 449 456, 463 , 470 , 477, 484, 491 , 498 , 505, 512, 519 , 526 , 533, 540 547, 554 , 561 , 568, 575, 582 , 589 , 596, 603, 610 , 617 , 624 631- 632 148, 155 , 162 , 169, 176, 183 , 190 , 197, 204, 211 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 183, 190 , 197 , 204, 211, 218 , 225 , 232, 239, 246 , 253 , 260, 274 281, 288 , 295 , 302, 309, 316 , 323 , 330, 337, 344 , 351 , 358, 365 372, 379 , 386 386 1, 8, 15 , 22, 29, 36, 43, 50 , 57, 64, 71, 78, 85 , 267 64, 71, 78, 85, 92, 94 1, 8, 15 , 22, 29, 36, 43, 50 , 57 812 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 103 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90878 D C90879 M 53 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL-RIGHT EAR-RIGHT VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 81 APPEARANCE HUNCHED THIN EXCRETION FEW FECES NO FECES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 218, 225 15, 22, 29, 36,, 43, 50, 57, 64 , 71,. 78, 85, 92, 99, 106, 113 120, 127, 134 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 1, 8, 232, 239,, 246, 253 , 344, 351,. 358, 365 , 369 561 547, 554, 561 554, 561 545-547 547 545-547 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 813 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 104 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90880 3 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 DISCHARGE NASAL RED IN COLOR EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C90882 T 105 DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS 1, 8,, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 680 533, 582 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 540, 547, 554, 561, 568 575, 589, 596, 603, 610, 617, 624, 631 , 638, 645, 652, 659, 666 673, 687, 694, 701, 708, 715, 722, 729 , 731 253, 708 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 260 , 267, 274, 281, 288, 295 302, 309, 316,. 323, 330, 337, 344, 351 , 358, 365, 372, 379, 386 393, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533 , 540, 547, 554, 561, 568 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 652, 659 814 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 105 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90882 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90883 T 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C90884 T 105 EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 666, 673, 680, 687, 694, 701, 715, 722 , 729, 731 225, 232, 239,. 246, 253, 260, 267, 274 , 281, 288, 295, 302 , 309 316, 323, 330, 337, 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421,. 428, 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519, 526, 533, 540, 547 , 554, 561, 568, 575 , 582 589, 596, 603,. 610, 617, 624, 631, 638 , 645, 652, 659, 666 , 673 680, 687, 694, 701, 708, 715, 722, 729 , 732 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400 547, 554, 561,. 568, 575, 582, 589, 596 , 603, 610, 617, 624 , 631 638, 645, 652, 659, 666, 673, 680, 687 , 694, 701, 708, 715 , 722 729, 731 815 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 106 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90884 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90885 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C90886 M 91 APPEARANCE MALOCCLUSION THIN EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 649, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729, 731 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554 589, 596, 603, 610, 617, 624, 630-631 630-631 630-631 816 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 107 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90886 M 91 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB PAW-HIND-RIGHT TAIL NORMAL NO REMARKABLE OBSERVATIONS C90887 M WART LIKE LESION(S) PAW-HIND-LEFT WEEK 89 69 APPEARANCE HUNCHED BEHAVIOR HYPOACTIVE EXCRETION NO FECES EYES SQUINTED PERI-ORBITAL DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 630631 603, 610, 617, 624 , 630- 631 547, 554 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 561, 568 , 575 582 617, 477 477 477 624,630-631 477 817 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 108 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90887 M 69 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS M 81 APPEARANCE MALOCCLUSION THIN EXCRETION FEW FECES NO FECES SKIN & PELAGE RED HAIR COAT PERINEAL AREA DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 470 477 477 477 1, 8, 15 , 22, 29, 36, 43 , 50 , 57, 64, 71 , 78, 85, 92, 99, 106 113, 120 , 127, 134 , 141, 148 , 155, 162, 169, 176, 183, 190, 197 204, 211 , 218, 225 , 232, 239 , 246, 253, 260, 267, 274, 281, 288 295, 302 , 309, 316 , 323, 330 , 337, 344, 351, 358, 365, 372, 379 386, 393 , 400, 407 , 414, 421 , 428, 435, 442, 449, 456, 463 43, 50, 57, 64 , 71 , 78, 85, 92, 99 , 106, 113,r 120, 127 , 134, 141 148, 155 , 162, 169 , 176, 183 , 190, 197, 204, 211, 218, 225, 232 239, 246 , 253, 260 , 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351 , 358, 365 , 372, 379, 386, 393, 400, 407, 414 421, 428 , 435, 442 , 449, 456 , 463, 470, 477, 484, 491, 498, 505 512, 519 , 526, 533 , 540, 547 , 554, 558, 561 558, 561 547, 554, 558 561 561 818 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 109 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90888 M 81 NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL WEEK 79 C90889 T 105 EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-LEFT LIMB-HIND-LEFT PAW-FRONT-RIGHT PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8, 15, 22, 29, 36 547, 554, 558, 561 218, 582, 701 701, 708 701, 708 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729-730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 225, 232 , 239, 246, 253, 260, 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351, 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442, 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519 526, 533, 540, 547 , 554, 561, 568, 575C 819 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 110 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90890 1 C90891 1 C90892 M 4 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 77 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL-RIGHT SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 1, 8, 15, 22, 24 526, 532-533 532-533 526 532-533 526, 532-533 526, 532-533 526, 532-533 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 820 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 111 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90892 M 77 NORMAL NO REMARKABLE OBSERVATIONS C90893 T 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C90894 M 98 BEHAVIOR HYPOACTIVE RECUMBENT STERNAL RESPIRATION LABORED SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 715, 722 1, 8, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 729, 731 684 684 684 680, 684 568, 575, 582, 680, 684 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 99, 106 821 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 112 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90894 M 98 NORMAL NO REMARKABLE OBSERVATIONS D 94 NORMAL NO REMARKABLE OBSERVATIONS C90896 M 72 APPEARANCE HUNCHED MALOCCLUSION MISSING TEETH THIN BEHAVIOR HYPOACTIVE DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554, 561 589, 596, 603, 610 , 617, 624, 631, 638 , 645, 652, 659, 666, 673 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589 , 596, 603, 610, 617 , 624, 631, 638, 645, 652 657 477, 484 , 491, 498 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148 155, 162 , 169, 176, 183, 190 , 197 , 204 , 211 , 218 , 225 , 232 , 239 246, 253 , 260, 267, 274, 281 , 288 , 295 , 302 , 309 , 316 , 323 , 330 337, 344 , 351, 358, 365, 372 , 379 , 386 , 393 , 400 , 407 , 414 , 421 428, 435 , 442, 449, 456, 463 , 470 , 477 , 484 , 491 , 498 190, 197 , 204, 211, 218, 225 , 232 , 239 , 246 477, 484 , 491, 498 477, 484, 491, 498 822 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 113 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90896 M 72 EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C90897 D 95 EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS C90898 M MASS(ES) MOUTH WEEK 82 62 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION NO FECES DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 498 1, 8, 15, 22, 29, 36, 43, 50 22, 29 1, 8,r 15, 36, 43, 50, 57,r 64, 71, 78, 85, 92, 99, 106, 113, 120 127, 134, 141, 148 , 155, 162, 169, 176 , 183, 190, 197, 204, 211 218, 225, 232, 239 , 246, 253, 260, 267 , 274, 281, 288, 295, 302 309, 316, 323, 330 , 337, 344, 351, 358 , 365, 372, 379, 386, 393 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477, 484 491, 498, 505, 512 , 519, 526, 533, 540 , 547, 554, 561, 575, 582 589, 596, 603, 610 , 617, 624, 631, 638 , 645, 652, 659, 661 568 428, 431 431 431 823 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 114 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90898 M 62 EYES SQUINTED PERI-ORBITAL SKIN & PELAGE PALE ENTIRE BODY RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C90899 M 104 APPEARANCE PROTRUDING PENIS BEHAVIOR HYPOACTIVE RECUMBENT LATERAL EXCRETION FEW FECES EYES RED DISCHARGE EYES DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 428, 431 431 428, 431 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421 680, 687, 694 722 722 715, 722 722 824 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 115 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M3 (CONTINUED FROM PREVIOUS PAGE) C90899 M 104 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL WEEK 69 C90900 T 105 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568, 575, 582 722 708 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 825 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT C90841 C90867 C90889 M3 575 83 MASS(ES) VENTRAL-ABDOMINAL WEEK 83 M3 491 71 TO 624 90 MASS(ES) DORSAL WEEK 64 631 91 MASS(ES) TO 731 105 DORSAL WEEK 64 M3 575 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 10 X 10MM 15 X 15MM 20 X 20MM 10 X 10MM Covance 6329-212 3M T-6316.1 PAGE: 116 826 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 117 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90901 D 74 APPEARANCE HUNCHED EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C90902 4 53 NORMAL NO REMARKABLE OBSERVATIONS C90903 D 101 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 510, 512 510, 512 1, 8,r 15, 22, 113, 120, 127 204, 211, 218 295, 302, 309 386, 393, 400 477, 484, 491 9, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 498, 505 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 19 0, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 28 1, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 36 7 561, 568, 575,. 582, 589, 596, 603, 610 , 617, 624, 659, 666, 673 680, 687, 694, 701, 703 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 631 638, 645, 652 827 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 118 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90904 T 105 DISCHARGE NASAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS 3 C90906 M 14 NORMAL NO REMARKABLE OBSERVATIONS 102 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION NO FECES EYES RED DISCHARGE EYES 729-730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 94 713 713 713 713 708,713 828 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 119 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90906 M 102 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C90907 3 C90908 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 708, 713 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 94 155, 162, 169, 176, 302, 309, 316, 323, 330, 337, 344, 351, 358 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729-730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 183, 190 , 1 97, 204, 211, 218 , 225 232, 239, 246,. 253, 260, 267, 274, 281 , 2 88, 295 829 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 120 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90909 4 53 NORMAL NO REMARKABLE OBSERVATIONS C90910 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90911 T 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) MOUTH WEEK 95 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 575, 582 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 722, 729, 731 830 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 121 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90912 M 82 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC SENSITIVE TO TOUCH DISCHARGE ORAL RED IN COLOR EYES SQUINTED PERI-ORBITAL SKIN & PELAGE PALE ENTIRE BODY YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 561, 564, 568 547, 554, 561, 564, 568 564, 568 564, 568 498 547, 554, 561, 564, 568 564, 568 568 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 505, 512, 519, 526, 533 , 540 831 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 122 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90913 4 53 APPEARANCE MALOCCLUSION C90914 M MISSING TEETH NORMAL NO REMARKABLE OBSERVATIONS APPEARANCE HUNCHED EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 50, 57, 64, 71 , 78, 85, 92, 99, 106, 113 , 120 , 127 , 134, 14 1 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 211, 218, 225, 232 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351, 358, 365 , 367 232, 239 , 246, 253, 260, 267 , 274, 281, 288, 295, 302, 309, 316 323, 330 , 337, 344, 351, 358 , 365, 367 1, 8, 15 , 22, 29, 36, 43 692, 694 , 696 692, 694 , 696 547, 554 , 561, 568, 575, 582 , 589, 596, 603, 610, 617, 624, 631 638, 645 , 652, 659, 666, 673 , 680, 687, 692, 694, 696 659, 666 , 673, 680, 687, 692 , 694, 696 1, 8, 15 , 22, 29, 36, 43 , 50 , 57, 64, 71 , 78, 85, 92, 99, 106 113, 120 , 127, 134, 141, 148 , 155, 162, 169, 176, 183, 190, 197 204, 211 , 218, 225, 232, 239 , 246, 253, 260, 267, 274, 281, 288 295, 302 , 309, 316, 323, 330 , 337, 344, 351, 358, 365, 372, 379 386, 393 , 400, 407, 414, 421 , 428, 435, 442, 449, 456, 463, 470 477, 484 , 491, 498, 505, 512 , 519 832 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 123 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90914 M 100 MASS(ES) LIMB-HIND-LEFT WEEK 76 C90915 D 96 DISCHARGE NASAL RED IN COLOR SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C90916 T 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 526C, 533C, 54 0C, 547C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 692C, 694C, 696C 344, 659, 666 631, 638 505 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 351 , 358, 365, 372, 379 , 386 393, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498,. 512, 519, 526, 533, 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603, 610, 617, 624, 645 , 652 589, 596 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 1 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 2 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 3 833 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 124 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90916 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90917 3 C90918 4 14 NORMAL NO REMARKABLE OBSERVATIONS 53 NORMAL NO REMARKABLE OBSERVATIONS C90919 D 79 APPEARANCE MALOCCLUSION SWOLLEN PAWS-FRONT SKIN & PELAGE SORE/SCAB EARS NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400,, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582,, 603, 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,, 694, 701, 708, 715, 722 , 729, 731 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 94 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365, 367 477 526, 533, 540, 547, 551 526, 533, 540, 547, 551 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400,, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 484, 491, 498,, 505, 512, 519 834 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 125 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90920 M 104 APPEARANCE HUNCHED BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION NONFORMED FECES EYES SQUINTED PERI-ORBITAL RESPIRATION LABORED SKIN & PELAGE SORE/SCAB SCROTUM TAIL NORMAL NO REMARKABLE OBSERVATIONS 727 727 727 533, 575, 582, 727 727 727 701, 708, 715, 722 , 727 603, 610, 617, 624 , 631, 638, 645, 652 , 659, 666, 673, 680 , 687 694, 701 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 835 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 126 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90920 M 104 NORMAL NO REMARKABLE OBSERVATIONS C90921 3 C90922 M 14 NORMAL NO REMARKABLE OBSERVATIONS 64 APPEARANCE THIN BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NONFORMED FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 540, 547, 554,, 561, 568 589, 596 1, 8,r 15, 22, 29, 36, 43,. 50, 57, 64, 71, 78, 85, 92, 94 442 442 414 414, 421, 428, 435, 442 155 414, 428, 435, 442 414 428, 435, 442 1, 8,r 15, 22, 29, 36, 43,. 50, 57, 64, 71, 78, 85, 92, 99, 106 836 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 127 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90922 M 64 NORMAL NO REMARKABLE OBSERVATIONS C90923 M MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 29 86 APPEARANCE THIN BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 162, 169, 176, 183, 190, 225, 232 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 197, 204, 211, 218 594, 596, 601 594, 596, 601 589, 594, 596, 601 594, 596, 601 589, 594, 596, 601 365, 372 1, 8,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218,. 225, 232, 239, 246, 295, 302, 309, 316,. 323, 330 837 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 128 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90923 M 86 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 37 4 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C90925 M 93 BEHAVIOR HYPOACTIVE RECUMBENT STERNAL DISCHARGE GENITAL RED IN COLOR NASAL RED IN COLOR EXCRETION FEW FECES DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 337, 344, 351, 358 , 379, 386, 393, 400 , 407, 414, 421, 428 , 435 442, 449, 456, 463 , 470, 477,. 484, 491 , 498, 505, 512, 519 , 526 533, 540, 547, 554 , 561, 568,. 575, 582 253, 260C, 267C, 274C, 281C, 288C 197, 204, 211, 260 , 267, 295, 302, 309 , 316, 323, 330, 337 , 365 367 1, 8,r 15, 22, 29, 36, 43,r 50,. 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148,. 155, 162 , 169, 176, 183, 190 , 218 225, 232, 239, 246 , 253, 274, 281, 288 , 344, 351, 358 645-646 645-646 646 646 645-646 838 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 129 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90925 M 93 EYES RED DISCHARGE EYES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C90926 T 105 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT YELLOW HAIR COAT DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 645-646 645-646 1, 8, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 589, 596, 603, 610, 617 , 624, 631, 638 728-730 728-730 728-730 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708 728- 730 839 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 130 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90926 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90927 T MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 89 VENTRAL-THORACIC-LEFT WEEK 95 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 295, 302, 309, 316 , 323 330, 337, 344, 351, 358, 365, 372, 379 , 386, 393, 400, 407 , 414 421, 428, 435,. 442, 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526,. 533, 540, 547, 554, 561 , 568, 575, 582, 589 , 715 722 260, 267, 274, 281, 288 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 631 , 638, 645, 652 617C, 624C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C 840 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 131 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90928 T 105 APPEARANCE MALOCCLUSION THIN EYES DISCOLORED/LIGHT RED EYES RESPIRATION AUDIBLE SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 701, 708, 715, 722 , 729- 730 617, 624, 631, 638 , 645, 652, 659, 666 , 673, 680, 687, 694 , 701 708, 715, 722,. 729 -730 582, 589, 596 582, 589, 596 582, 589, 596 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575 841 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 132 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90929 D 69 APPEARANCE MALOCCLUSION C90930 M NORMAL NO REMARKABLE OBSERVATIONS 90 APPEARANCE CONVULSIONS MALOCCLUSION MISSING TEETH PROTRUDING PENIS SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 71, 78, 92, 99 , 106, 113 , 120, 127 , 134, 141,r 148,r 155,, 162, 169 176, 183 , 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274 , 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365 , 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456 , 463, 470, 477 1, 8, 15 , 22, 29, 36 , 43 , 50, 57, 64, 85 568 43, 50, 57, 64 , 71, 78, 85, 92 , 99 , 106, 113,r 120, 127,, 134, 141 148, 155 , 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246 , 253, 260, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358 , 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449 , 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540 , 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624 568, 575 , 582, 589, 596, 603, 610, 617, 624 589 582, 589 , 596, 603, 610, 617, 624 1, 8, 15, 22, 29, 36, 267, 274, 281 842 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 133 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90931 M 98 APPEARANCE LIMITED USE LIMBS-HIND PAWS-FRONT BEHAVIOR ATAXIC NORMAL NO REMARKABLE OBSERVATIONS C90932 4 53 NORMAL NO REMARKABLE OBSERVATIONS C90933 T 05 APPEARANCE MALOCCLUSION 680 680 680 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631 659, 666, 673 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547, 554, 561 638, 645, 652 1, 8, 15 , 22, 29, 36 , 43,r 50, 57, 64, 71 , 78 , 85, 92, 99, 106 113, 120 , 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211 , 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302 , 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 78, 85, 92, 99 , 106, 134, 141, 148 , 155, 162 , 169, 176 , 18 3, 19 197, 204 , 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 , 302 309, 316 , 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 , 393 400, 407 , 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 , 484 491, 498 , 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 , 575 582, 589 , 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 , 666 673, 680 , 687, 694, 701, 708, 715, 722, 729- 730 843 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 134 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90933 T 105 DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 88 C90934 M 103 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE DISCHARGE ORAL RED IN COLOR EXCRETION FEW FECES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 372, 379, 533 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 113, 120, 127, 281 288, 295 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 716, 718-719 718-719 716, 718-719 715-716, 718-719 715-716, 718-719 716, 718-719 844 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 135 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90934 M 103 NORMAL NO REMARKABLE OBSERVATIONS C90935 1 C90936 M MASS(ES) VENTRAL-ABDOMINAL WEEK 102 4 NORMAL NO REMARKABLE OBSERVATIONS 101 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701 70 8C, 715-716C, 718-719C 1, 8, 15, 22, 24 43, 50, 57, 64,r 71, 78, 85, 92, 99,r 106,r 113,r 120, 127,r 134, 141 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 211, 218, 225, 232 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393, 400, 407, 414 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484, 491, 498, 505 512, 519 , 526, 533, 540, 547 , 554, 561, 568, 575, 582, 589, 596 603, 610 , 617, 624, 631, 638 , 645, 652, 659, 666, 673, 680, 687 694, 701 , 703, 705 705 705 845 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 136 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90936 M 101 EXCRETION DISCOLORED URINE RED IN COLOR FEW FECES NO FECES SKIN & PELAGE PALE ENTIRE BODY ROUGH HAIR COAT SORE/SCAB PAW-HIND-LEFT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS M 103 APPEARANCE HUNCHED MALOCCLUSION EXCRETION NO FECES DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 705 703 705 705 703, 705 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 703, 705 1, 8, 15, 22, 29, 36 715 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148 155, 162 , 169, 176, 183, 190 , 197 , 204 , 211 , 218 , 225 , 232 , 239 246, 253 , 260, 267, 274, 281 , 288 , 295 , 302 , 309 , 316 , 323 , 330 337, 344 , 351, 358, 365, 372 , 379 , 386 , 393 , 400 , 407 , 414 , 421 428, 435 , 442, 449, 456, 463 , 470 , 477 , 484 , 491 , 498 , 505 , 512 519, 526 , 533, 540, 547, 554 , 561 , 568 , 575 , 582 , 589 , 596 , 603 610, 617 , 624, 631, 638, 645 , 652 , 659 , 666 , 673 , 680 , 687 , 694 701, 708 , 715 715 846 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 137 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90937 M 103 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C90938 M 69 APPEARANCE HUNCHED BEHAVIOR HYPOACTIVE RECUMBENT LATERAL EXCRETION FEW FECES SKIN & PELAGE BROWN HAIR COAT HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT BROWN SKIN VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 715 715 1, 8, 15, 22, 29, 36, 43, 50 477, 481 481 481 477 481 481 481 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 847 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 138 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90939 1 C90940 M 4 NORMAL NO REMARKABLE OBSERVATIONS 64 APPEARANCE HEAD TILT LEFT HUNCHED THIN BEHAVIOR ATAXIC HYPOACTIVE EXCRETION NO FECES EYES RED DISCHARGE EYES RESPIRATION LABORED SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 442 440, 442 440, 442 440, 442 440, 442 440, 442 440, 442 440, 442 440, 442 440, 442 435, 440, 442 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 848 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 139 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90940 M 64 NORMAL NO REMARKABLE OBSERVATIONS M 88 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL SKIN & PELAGE COLD TO TOUCH ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428 610 589, 596, 603, 609 -610 610 561, 568, 575, 582 , 589, 596, 603, 609 -610 610 609- 610 610 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 849 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 140 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90941 M 88 NORMAL NO REMARKABLE OBSERVATIONS C90942 1 4 NORMAL NO REMARKABLE OBSERVATIONS C90943 D 92 APPEARANCE MALOCCLUSION SWOLLEN MOUTH THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NO FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 1, 8,, 15, 22, 24 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148 155, 162 , 169, 176, 183, 190 , 197 , 204 , 211 , 218 , 225 , 232 , 239 246, 253 , 260, 267, 274, 281 , 288 , 295 , 302 , 309 , 316 , 323 , 330 337, 344 , 351, 358, 365, 372 , 379 , 386 , 393 , 400 , 407 , 414 , 421 428, 435 , 442, 449, 456, 463 , 470 , 477 , 484 , 491 , 498 , 505 , 512 519, 526 , 533, 540, 547, 554 , 561 , 568 , 575 , 582 , 589 , 596 , 603 610, 617 , 624, 629, 631, 638 -639 57, 64 631, 638-639 631, 638-639 629, 631 638-639 629, 631, 638-639 1, 8, 15, 22, 29, 36, 43, 50 850 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 141 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90944 T 105 NORMAL NO REMARKABLE OBSERVATIONS C90945 M 45 DISCHARGE NASAL RED IN COLOR RESPIRATION AUDIBLE SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 309 309 309 1, 8,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302 851 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 142 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90946 M 78 APPEARANCE MALOCCLUSION C90947 T THIN EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 50, 57, 64, 71 , 78, 85, 92, 99, 106 , 113 , 120 , 127 , 134 , 14 1 148, 155 , 162, 169,. 176,. 183, 190, 197, 204, 211, 218, 225, 232 239, 246 , 253, 260, 267,. 274, 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351,. 358,. 365, 372, 379, 386, 393, 400, 407, 414 421, 428 , 435, 442, 449,. 456, 463, 470, 477, 484, 491, 498, 505 512, 518 -519, 526, 533, 540 518- 519, 526, 533, 540 540 1, 8,r 15, 22, 29, 36, 43 561, 568, 575, 582 , 589, 596, 603, 610 , 617, 624, 631, 638, 645 652, 659, 666, 673 , 680, 687, 694, 701 , 708, 715, 722, 729, 731 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 852 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 143 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90948 T 105 APPEARANCE MALOCCLUSION EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB PAWS-HIND C90949 M NORMAL NO REMARKABLE OBSERVATIONS 13 APPEARANCE MALOCCLUSION THIN EXCRETION NONFORMED FECES 134 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680 687, 694, 701, 708, 715, 722, 729, 731 127 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589 , 596, 603, 610, 617 , 624, 631, 638, 645, 652 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 722, 729, 731 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 71, 78, 85-86 78, 85-86 85-86 853 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 144 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90949 M 13 SKIN & PELAGE BROWN HAIR COAT SCROTUM NORMAL NO REMARKABLE OBSERVATIONS C90950 4 53 NORMAL NO REMARKABLE OBSERVATIONS C90951 4 53 APPEARANCE MALOCCLUSION MISSING TEETH EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 85-86 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 120, 127, 134, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 29, 36, 43 1, 8, 15, 22, 50, 57, 64, 71, 78, 85, 92 99, 106, 113 854 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 145 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90952 M 59 BEHAVIOR RECUMBENT LATERAL EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SQUINTED PERI-ORBITAL RESPIRATION AUDIBLE NORMAL NO REMARKABLE OBSERVATIONS C90953 T NORMAL NO REMARKABLE OBSERVATIONS 411 407, 411 386 407, 411 411 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 393, 400 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 855 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 146 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90954 M 69 APPEARANCE THIN EXCRETION NO FECES SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE SWOLLEN TAIL-PROXIMAL NORMAL NO REMARKABLE OBSERVATIONS 477, 479 479 225, 477, 479 463, 470, 477, 479 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 722, 729, 732 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617 , 624, 631, 638, 645 , 652 856 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 147 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90956 T 105 APPEARANCE HUNCHED MALOCCLUSION THIN EXCRETION NO FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 727, 729 , 731 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148, 155, 162 169, 176 , 183, 190, 197, 204, 211, 218, 225, 232, 239, 246, 253 260, 267 , 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358 , 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449 , 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540 , 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631 , 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 715, 722 , 727, 729, 731 727, 729 , 731 727, 729, 731 729, 731 727, 729, 731 1, 8, 15, 22, 29, 36, 43, 50, 57, 64 857 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 148 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90957 4 53 APPEARANCE MALOCCLUSION C90958 D MISSING TEETH NORMAL NO REMARKABLE OBSERVATIONS 94 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND 50, 57, 64 , 71,, 78, 85, 92, 99, 106, 113 , 120 , 127, 134 , 141 148,. 155, 162, 169, 176, 183 , 190, 197, 204, 211, 218, 225, 232 239,. 246, 253, 260, 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365 , 367 302, 309, 316, 323, 330, 337 , 344, 351, 358, 365, 367 1, 8, 15, 22, 29, 36, 43 50, 57, 64, 71,r 78, 85, 92, 99, 106 , 113 , 120 , 127 , 134 , 141 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 211, 218, 225, 232 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393, 400, 407, 414 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484, 491, 498, 505 512, 519 , 526, 533, 540, 547 , 554, 561, 568, 575, 582, 589, 596 603, 610 , 617, 624, 631, 638 , 645, 652, 656 477, 484 , 491, 498, 505, 512 , 519, 526, 533, 540, 547, 554, 561 568, 575 , 582, 589, 596, 603 , 610, 617, 624, 631, 638, 645, 652 656 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 656 858 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 149 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90958 D 94 NORMAL NO REMARKABLE OBSERVATIONS C90959 M 86 APPEARANCE HEAD TILT LEFT BEHAVIOR ATAXIC EXCRETION FEW FECES EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8, 15, 22, 29, 36, 43 600 600 600 600 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596 859 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 150 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90960 3 1 C90962 M 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 71 APPEARANCE HUNCHED MALOCCLUSION THIN BEHAVIOR HYPOACTIVE EXCRETION NO FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 1, 8, 15, 22, 24 477, 484, 488-489, 491-492 295 491-492 488-489, 491-492 491-492 488-489, 491-492 488-489, 491-492 477, 484, 488-489, 491-492 1, 8, 15 113, 120 204, 211 302, 309 393, 400 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 860 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 151 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90963 M 73 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC HYPOACTIVE SKIN & PELAGE COLD TO TOUCH ENTIRE BODY ROUGH HAIR COAT SORE/SCAB HEAD-MAXILLARY-LEFT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C90964 M 74 APPEARANCE MALOCCLUSION PARALYSIS LIMB-FRONT-LEFT SWOLLEN PAWS-HIND THIN 477, 484, 509 498, 505, 507, 509 509 507, 509 507, 509 477, 484, 491, 498, 505, 507, 509 386, 393, 400 507, 509 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470 99, 106, 113, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365,. 372, 379,, 386, 393, 400, 407,. 414, 421,. 428, 435,. 442, 449 456,. 463, 470 120 505, 512, 515 512, 515 861 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 152 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90964 M 74 EYES DISCOLORED/LIGHT RED EYE-LEFT OPAQUE EYE-RIGHT SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB HEAD-MAXILLARY-RIGHT MOUTH PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS C90965 T MASS(ES) MOUTH WEEK 67 APPEARANCE MALOCCLUSION DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 512, 515 512, 515 515 8 477, 484 302 , 309, 316, 323, 330 , 337, 344 351 , 358, 365, 372, 379 , 477, 484, 491, 498, 505, 512, 515 1, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85 , 92, 127, 134, 141 148 , 155, 162, 169, 176 , 183, 190, 197, 204, 211, 218, 225, 232 239 , 246, 253, 260, 267 , 274, 281, 288 463 , 470 50, 57, 64 , 71,r 78, 85, 92, 99 , 106 , 113, 120 , 127 , 134 , 141 148 , 155, 162, 169, 176 , 183, 190, 197, 204, 211, 218, 225, 232 239 , 246, 253, 260, 267 , 274, 281, 288, 295, 302, 309, 316, 323 330 , 337, 344, 351, 358 , 365, 372, 379, 386, 393, 400, 407, 414 421 , 428, 435, 442, 449 , 456, 463, 470, 477, 484, 491, 498, 505 512 , 519, 526, 533,. 540 , 547, 554, 561, 568, 575, 582, 589, 596 603 , 610, 617, 624, 631 , 638, 645, 652, 659, 666, 673, 680, 687 862 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 153 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90965 T 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C90966 1 4 NORMAL NO REMARKABLE OBSERVATIONS C90967 4 53 NORMAL NO REMARKABLE OBSERVATIONS C90968 T 105 APPEARANCE MALOCCLUSION THIN EXCRETION NO FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 694, 701, 708, 715, 722, 729, 731 729, 731 568, 575, 582, 589, 596 1, 8, 15, 22, 29, 36, 43 1, 8, 15, 22, 24 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 367 589, 596, 603,. 610, 617, 624, 631, 638 , 645, 652, 659, 666 , 673 680, 687, 694, 701, 708, 715, 722, 729 -730 477 477 477, 484 477 863 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 154 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90968 T 105 SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-LEFT PAWS-HIND VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C90969 D 85 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT GENERALIZED DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730 477 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 568, 575 568 568, 575 568 568, 575 864 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 155 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90969 D 85 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS M 100 BEHAVIOR HYPOACTIVE EXCRETION NONFORMED FECES EYES SQUINTED PERI-ORBITAL RESPIRATION IRREGULAR SKIN & PELAGE COLD TO TOUCH ENTIRE BODY SORE/SCAB DORSAL PAW-HIND-LEFT DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 540, 547, 554, 561, 568, 575, 582, 589 , 593 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533 697 344 697 697 697 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 697 666, 673, 680, 687, 694, 697 865 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 156 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M4 (CONTINUED FROM PREVIOUS PAGE) C90970 M 100 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 56 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 351, 358, 365, 372, 379 386C, 393C, 400C, 407C, 414C, 421C , 428C, 435C, 442C, 449C , 456C 463C, 470C, 477C, 484C, 491C, 498C , 505C, 512C, 519C, 526C , 533C 54 0C,. 547C, 554C, 561C, 568C, 575C , 582C, 589C, 596C, 603C , 610C 617C, 624C, 631C, 638C, 645C, 652C , 659C, 666C, 673C, 680C , 687C 694C, 697C 866 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C90914 C90923 C90927 M4 526 76 MASS(ES) LIMB-HIND-LEFT WEEK 76 533 77 TO 568 82 MASS(ES) LIMB-HIND-LEFT WEEK 76 575 83 MASS(ES) TO 696 100 LIMB-HIND-LEFT WEEK 76 M4 260 38 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 37 M4 617 89 TO 624 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 659 95 MASS(ES) TO 701 101 VENTRAL-THORACIC-LEFT WEEK 95 708 102 MASS(ES) TO 722 104 VENTRAL-THORACIC-LEFT WEEK 95 729 105 MASS(ES) TO 730 105 VENTRAL-THORACIC-LEFT WEEK 95 50X40MM 50 X 50MM 65 X 60MM 10X 10MM 10 X 10MM 40 X 30MM 55 X 60MM 70 X 65MM 867 Covance 6329-212 3M T-6316.1 PAGE: 157 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C90933 M4 610 88 TO 624 90 MASS(ES) AXILLARY REGION-LEFT WEEK 88 631 91 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 88 645 93 MASS(ES) AXILLARY REGION-LEFT WEEK 88 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 88 659 95 TO 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 88 694 100 MASS(ES) TO 701 101 AXILLARY REGION-LEFT WEEK 88 708 102 MASS(ES) TO 715 103 AXILLARY REGION-LEFT WEEK 88 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 88 10 X 10MM 35 X 40MM 40 X 40MM 60 X 60MM 80 X 60MM 100 X 70MM 100 X 110MM 110 X 110MM 868 Covance 6329-212 3M T-6316.1 PAGE: 158 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C90933 C90934 C90970 M4 729 105 MASS(ES) TO 730 105 AXILLARY REGION-LEFT WEEK 88 M4 708 102 MASS(ES) TO 719 103 VENTRAL-ABDOMINAL WEEK 102 M4 386 56 TO 484 70 MASS(ES) DORSAL WEEK 56 491 71 TO 526 76 MASS(ES) DORSAL WEEK 56 533 77 MASS(ES) TO 697 100 DORSAL WEEK 56 120 X 110MM 25 X 40MM 10 X 10MM 15 X 15MM 20 X 25MM Covance 6329-212 3M T-6316.1 PAGE: 159 869 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 160 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90971 2 C90972 2 C90973 6 C90974 6 C90975 6 C90976 6 C90977 6 C90978 6 C90979 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 870 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 161 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90980 6 C90981 6 C90982 6 C90983 6 C90984 1 C90985 6 C90986 6 C90987 6 C90988 2 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50, 54 871 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 162 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90989 6 C90990 6 C90991 6 C90992 6 C90993 D C90994 6 C90995 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15 , 22 , 29, 36 , 43, 50 1, 8, 15 , 22 , 29, 36 , 43, 50 1, 8, 15 , 22 , 29, 36 , 43, 50 1, 8, 15 , 22 , 29, 36 , 43, 50 50, 53 1, 8, 15 , 22 , 29, 36 , 43 1, 8, 15 , 22 , 29, 36 , 43, 50 15,r 22, 29, 36, 43 50 1, 8 872 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 163 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C90996 2 C90997 6 C90998 6 C90999 6 C91000 6 C91001 6 C91002 6 C91003 D 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 7 APPEARANCE CONVULSIONS BEHAVIOR HYPOACTIVE SKIN & PELAGE COLD TO TOUCH ENTIRE BODY PALE ENTIRE BODY 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 43 43 43 43 873 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 164 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 (CONTINUED FROM PREVIOUS PAGE) C91003 D 7 NORMAL NO REMARKABLE OBSERVATIONS C91004 6 8 NORMAL NO REMARKABLE OBSERVATIONS C91005 1 4 NORMAL NO REMARKABLE OBSERVATIONS C91006 6 8 NORMAL NO REMARKABLE OBSERVATIONS C91007 6 8 NORMAL NO REMARKABLE OBSERVATIONS C91008 6 8 NORMAL NO REMARKABLE OBSERVATIONS C91009 6 8 NORMAL NO REMARKABLE OBSERVATIONS C91010 6 8 NORMAL NO REMARKABLE OBSERVATIONS C91011 6 8 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 45 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 874 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 165 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91012 6 C91013 6 C91014 6 C91015 6 C91016 6 C91017 2 C91018 6 C91019 6 C91020 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 875 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 166 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91021 6 C91022 6 C91023 6 C91024 1 C91025 6 C91026 6 8 APPEARANCE MALOCCLUSION THIN DISCHARGE NASAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 46, 50 50 46, 50 1, 8, 15, 22, 29, 36, 43 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 8, 15,, 22, 29,r 36,r 43, 50 1, 8, 15, 22, 29, 36, 43, 50 876 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 167 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91027 6 C91028 6 C91029 M 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 7 APPEARANCE CONVULSIONS BEHAVIOR VOCALIZATION DISCHARGE UNKNOWN RED IN COLOR RESPIRATION LABORED SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 49 49 49 49 49 1, 8, 15, 22, 29, 36, 43 877 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 168 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91030 6 C91031 6 C91032 6 C91033 1 C91034 6 C91035 6 C91036 6 C91037 1 C91038 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 1, 8, 15, 22, 29, 36, 43, 50 878 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 169 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91039 6 C91040 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 879 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 170 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91041 M 61 APPEARANCE THIN EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT VENTRAL-CERVICAL ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91042 D 93 APPEARANCE CONVULSIONS SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 421 407, 414, 421 414, 421 407, 414, 421 421 421 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 603, 610 638, 645, 650 880 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 171 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91042 D 93 NORMAL NO REMARKABLE OBSERVATIONS M 85 NORMAL NO REMARKABLE OBSERVATIONS C91044 M MASS(ES) INGUINAL REGION-RIGHT WEEK 75 64 APPEARANCE HEAD TILT RIGHT HUNCHED THIN DISCHARGE ORAL CLEAR DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 617, 624, 631 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C 589-590C 443 443 435, 442-443 57 881 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 172 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91044 M 64 DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C91045 M 51 APPEARANCE PARALYSIS LIMB-FRONT-LEFT BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NONFORMED FECES EYES RED DISCHARGE EYE-LEFT EYES DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 183 443 1, 8,r 15, 22, 29, 36 , 43,r 50, 64, 71, 78 , 85,, 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351,. 358, 365, 372, 379,. 386, 393 400, 407, 414, 421, 428 120 351, 353 351, 353 351 92, 99, 106, 113, 281, 288, 295, 302, 309, 316, 323, 330, 337 344 351, 353 882 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 173 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91045 M 51 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C91046 M C91047 U 7 EYES RED DISCHARGE EYE-LEFT RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 351, 353 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 127, 134, 141 148, 155, 162,, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232 239, 246, 253, 260, 267, 274 43-44 43-44 1, 8, 15, 22, 29, 36 57, 64, 71, 78 , 85, 92, 99, 106, 113, 120, 127, 134, 141, 148 155, 162 , 169, 176, 183, 190 , 197 , 204 , 211 , 218 , 225 , 232 , 239 246, 253 , 260, 267, 274, 281 , 288 , 295 , 302 , 309 , 316 , 323 , 330 337, 344 , 351, 358, 365, 372 , 379 , 386 , 393 , 400 , 407 , 414 , 421 428, 435 , 442, 449, 456, 463 , 470 , 477 , 484 , 491 , 498 , 505 , 512 519, 526 , 533, 540, 547, 554 , 561 , 568 , 575 , 582 , 589 , 596 , 603 610, 617 , 624, 631, 638, 645 , 652 , 659 , 666 , 673 , 680 , 687 , 694 701, 708 , 715, 722, 729, 732 1, 8, 15 , 22, 29, 36 , 43 , 50 883 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 174 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91048 M 80 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NO FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT GENERALIZED NORMAL NO REMARKABLE OBSERVATIONS 547, 554 547, 554 547 554 547, 554 547, 554 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 884 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 175 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91049 D 43 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL BEHAVIOR RECUMBENT LATERAL DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES RESPIRATION IRREGULAR SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 288, 295 295 295 288, 295 281 295 295 295 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274 885 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 176 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91050 D 103 APPEARANCE MALOCCLUSION C91051 M SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) DORSAL WEEK 95 89 APPEARANCE THIN BEHAVIOR HYPOACTIVE SENSITIVE TO TOUCH EXCRETION NO FECES 141, 148, 155, 162 , 169, 176, 183, 190 , 197, 204, 211, 218 , 225 232, 239, 246, 253 , 260, 267, 274, 281 , 288, 295, 302, 309 , 316 323, 330, 337, 344 , 351, 358, 365, 372 , 379, 386, 393, 400 , 407 414, 421, 428, 435 , 442, 449, 456, 463 , 470, 477, 484, 491 , 498 505, 512, 519, 526 , 533, 540, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617 , 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701, 708 , 715, 719 652, 659, 666, 673 , 680, 687, 694 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 719 617-618 617-618 617-618 617-618 886 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 177 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91051 M 89 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB PAW-HIND-LEFT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS M 68 APPEARANCE HEAD TILT RIGHT SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB PAW-HIND-LEFT VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 617-618 547, 554, 561 568, 575, 582, 589, 596, 603, 607, 610 , 617- 618 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540 463, 470 449, 456, 463, 470 449, 456, 463, 470 211, 218, 225 463, 470 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 232, 239,. 246, 253, 260, 267, 274 , 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365 , 372, 379, 386, 393, 400 407, 414, 421,. 428, 435, 442 887 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 178 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91053 D 83 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C91054 U 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) NOSE WEEK 79 498, 505, 547, 554, 561, 568, 575, 580 456, 463, 470 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 477, 484 , 491 512, 519, 526, 533 , 540 680, 687, 694, 701, 708, 715, 722, 729, 732 729, 732 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 568, 575 , 582 589, 596, 603, 610 , 617, 624, 631, 638 , 645, 652, 659, 666 , 673 547, 554, 561 888 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 179 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91055 D 82 APPEARANCE HEAD TILT RIGHT MALOCCLUSION BEHAVIOR HYPOACTIVE RECUMBENT STERNAL EXCRETION FEW FECES NO FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 564-566 566, 568-569 569 569 564 565-566, 568-569 569 566, 568-569 566, 568-569 564-566, 568-569 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 889 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 180 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91056 U 105 APPEARANCE MALOCCLUSION SWOLLEN MOUTH DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYES RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD WEEK 102 71, 78, 85 , 92, 99, 106, 211, 218, 225, 232, 239, 246, 253, 260 267 , 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358 , 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449 , 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540 , 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631 , 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722 , 729, 732 56- 57, 64 56-57, 64 56-57 56-57 1, 8,, 15, 22, 29, 36, 43,, 50, 113, 120, 127, 134, 141, 148, 155 162, 169, 176,, 183, 190, 197, 204 708C, 715C, 722C, 729C, 732C 890 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 181 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91057 D 73 NORMAL NO REMARKABLE OBSERVATIONS M 83 APPEARANCE MALOCCLUSION SWOLLEN MOUTH SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) MOUTH WEEK 83 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 510 575 575 22, 29 1, 8, 15, 36, 43, 50, 57,r 64, 71, 78, 85, 92, 99, 106, 113 , 120 127, 134, 141,. 148, 155, 162, 169, 176 , 183, 190, 197, 204 , 211 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288, 295 , 302 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 421, 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505,. 512, 519, 526, 533,. 540 , 547, 554, 561, 568 575 891 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 182 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91059 M 83 APPEARANCE THIN DISCHARGE GENITAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SQUINTED PERI-ORBITAL RESPIRATION LABORED SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA GENERALIZED RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 567-568, 575-576 106, 239, 274 567-568, 575-576 232, 239, 246, 253 576 576 568, 575 576 567 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113 120, 127, 134, 141 , 148, 155, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225, 260 , 267, 281, 288, 295 , 302, 309, 316, 323 , 330 337, 344, 351, 358 , 365, 372, 379, 386 , 393, 400, 407, 414 , 421 428, 435, 442, 449 , 456, 463, 470, 477 , 484, 491, 498, 505 , 512 892 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 183 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91059 M 83 NORMAL NO REMARKABLE OBSERVATIONS C91060 U 105 EYES DISCOLORED/LIGHT RED EYE-RIGHT RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91061 D 90 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 87 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 519, 526, 533, 540, 547, 554, 561 582, 589 596, 603, 610, 617 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687 694, 701, 708, 715, 722, 729, 732 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596 603C, 610C, 617C, 624C, 627C 893 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 184 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91062 M 93 APPEARANCE HEAD TILT LEFT HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS 631, 638, 645 638, 645 568, 575, 582, 589 , 596, 603, 608, 610 -611, 617, 624, 631, 638 645 617, 624, 631, 638 , 645 568, 575, 608, 610 -611, 617, 624, 631, 638, 645 638, 645 638, 645 1, 8,r 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 894 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 185 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91063 M 100 APPEARANCE THIN EXCRETION FEW FECES EYES PALE EYES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C91064 D 97 NORMAL NO REMARKABLE OBSERVATIONS 680, 683, 687, 691, 694, 697 680, 683, 687, 691, 694, 697 680, 683, 687, 691, 694, 697 680, 683, 687, 691, 694, 697 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 895 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 186 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91064 D 97 NORMAL NO REMARKABLE OBSERVATIONS C91065 U 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS M 86 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE SENSITIVE TO TOUCH EXCRETION FEW FECES SKIN & PELAGE COLD TO TOUCH ENTIRE BODY DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659, 666, 673, 679 120, 127, 134, 141 , 148, 155, 162, 169 , 176, 183, 190, 197, 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288, 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379, 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470, 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540, 547, 554, 561, 568 575, 582, 589, 596 , 603, 610, 617, 624 , 631, 638, 645, 652, 659 666, 673, 680, 687 , 694, 701, 708, 715 , 722, 729, 732 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113 589, 596, 598 547, 554, 561, 568 , 575, 582, 589, 596 , 598 598 598 547, 554, 561, 568 , 575, 582, 589, 596 , 598 598 896 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 187 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91066 M 86 NORMAL NO REMARKABLE OBSERVATIONS D 100 NORMAL NO REMARKABLE OBSERVATIONS C91068 M 12 APPEARANCE HUNCHED THIN DISCHARGE NASAL RED IN COLOR EXCRETION LIQUID FECES NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 696 71, 78 57, 64, 71, 78 43, 50 64 71, 78 1, 8, 15, 22, 29, 36 897 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 188 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91069 D 84 NORMAL NO REMARKABLE OBSERVATIONS C91070 U 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91071 U 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 585 729, 732 456, 463 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 470, 477, 484 491, 498, 505,. 512, 519, 526, 533, 540 , 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631 , 638, 645, 652, 659, 666 673, 680, 687,. 694, 701, 708, 715, 722 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645, 652 898 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 189 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91071 U 105 NORMAL NO REMARKABLE OBSERVATIONS C91072 U 105 EXCRETION FEW FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91073 U MASS(ES) MOUTH WEEK 104 105 APPEARANCE SWOLLEN PAW-HIND-LEFT DISCHARGE GENITAL RED IN COLOR DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659, 666,, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 728-729, 732 728-729, 732 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722 728-729, 732 673, 680, 687, 694, 701 365, 379, 386 899 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 190 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91073 U 105 EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB PAW-HIND-LEFT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91074 M 102 BEHAVIOR SENSITIVE TO TOUCH EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-RIGHT DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 372 729, 732 449, 456, 463,. 470, 568, 575, 582, 589 , 596, 603, 610, 617 , 624 631, 638, 645,. 652, 659, 666, 673, 680 , 687, 694, 701, 708 , 715 722, 729, 732 624, 631, 638, 645, 652, 659, 666, 673 , 680, 687, 694, 701 , 708 715, 722, 729, 732 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 393, 400 , 407 414, 421, 428, 435, 442, 477, 484, 491 , 498, 505, 512, 519 , 526 533, 540, 547,. 554, 561 711 708 711 638, 645 900 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 191 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91074 M 102 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91075 M 78 BEHAVIOR HYPOACTIVE RECUMBENT STERNAL DISCHARGE GENITAL RED IN COLOR DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 711 708, 711 708 344 295 302, 309, 316, 323 , 330, 337, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505, 512 , 519, 526, 533, 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 701, 708, 711 687, 694, 701, 708 , 711 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 540 540 372 901 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 192 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91075 M 78 EXCRETION FEW FECES EYES RED DISCHARGE EYES RESPIRATION LABORED SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS U 105 APPEARANCE MALOCCLUSION DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 540 540 540 526, 533,540 1, 8, 15 , 22, 29, 36 , 43 , 50 , 57, 64, 71 , 78, 85, 92, 99, 106 113, 120 , 127, 134, 141, 148 , 155, 162, 169, 176, 183, 190, 197 204, 211 , 218, 225, 232, 239 , 246, 253, 260, 267, 274, 281, 288 295, 302 , 309, 316, 323, 330 , 337, 344, 351, 358, 365, 379, 386 393, 400 , 407, 414, 421, 428 , 435, 442, 449, 456, 463, 470, 477 484, 491 , 498, 505, 512, 519 43, 50, 57, 64 , 71, 78, 85, 92, 99 , 106, 113,r 120, 127 , 134, 141 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 211, 218, 225, 232 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393, 400, 407, 414 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484, 491, 498, 505 512, 519 , 526, 533, 540, 547 , 554, 561, 568, 575, 582, 589, 596 603, 610 , 617, 624, 631, 638 , 645, 652, 659, 666, 673, 680, 687 694, 701 , 708, 715, 722, 729 , 732 902 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 193 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91076 U 105 DISCHARGE GENITAL RED IN COLOR SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS M 84 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN MOUTH SKIN & PELAGE COLD TO TOUCH ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 491 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610, 617, 624 , 631, 638, 645, 652 , 659, 666, 673, 680 , 687 694, 701, 708, 715 , 722, 729, 732 631, 638, 645, 652 , 659, 666, 673, 680 , 687, 694, 701, 708 , 715 722, 729, 732 1, 8,r 15, 22, 29, 36 155, 162, 169, 176 , 183, 190, 197, 204 , 211, 218, 225, 232 , 239 246, 253, 260, 267 , 274, 281, 288, 295 , 302, 309, 316, 323 , 330 337, 344, 351, 358 , 365, 372, 379, 386 , 393, 400, 407, 414 , 421 428, 435, 442, 449 , 456, 463, 470, 477 , 484, 491, 498, 505 , 512 519, 526, 533,. 540 , 547, 554, 561, 568 , 575, 582, 588 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 588 547 588 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 903 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 194 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91077 M 84 NORMAL NO REMARKABLE OBSERVATIONS C91078 D 93 APPEARANCE LIMITED USE LIMB-HIND-RIGHT SWOLLEN PAW-HIND-RIGHT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C91079 M 85 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR ATAXIC HYPOACTIVE DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148 386, 393, 400, 407, 414, 421, 428 435, 442, 449, 456, 463, 470, 477, 484 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358 491, 498, 505,. 512, 519, 526, 533, 540 , 547, 554 649 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 561, 638 , 645 579, 582, 588-590 568, 575, 579, 582, 588-590 590 579, 582, 588-590 904 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 195 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91079 M 85 EYES RED DISCHARGE EYES RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS D 98 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB HEAD-ENTIRE PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 190, 288, 295 568, 575, 579, 582 , 588- 590 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 302 , 309 316, 323, 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519 , 526, 533, 540, 547 , 554, 561 679- 681 679- 681 435 547, 554 561,. 568, 575, 582, 589, 596 , 603, 610, 617, 624 , 631 638, 645 652, 659, 666, 673, 679- 681 1, 8, 15 113, 120 204, 211 295, 302 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 905 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 196 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M6 (CONTINUED FROM PREVIOUS PAGE) C91080 D 98 NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) EAR-LEFT WEEK 65 VENTRAL-ABDOMINAL WEEK 64 WEEK 76 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386 393, 400, 407, 414, 421, 428 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631 638, 645, 652, 659, 666, 673, 679- 681 442 449 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617 624, 631, 638, 645, 652, 659, 666, 673, 679- 681 906 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91043 C91056 C91061 M6 519 75 TO 526 76 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 533 77 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 568 82 TO 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 589 85 TO 590 85 MASS(ES) INGUINAL REGION-RIGHT WEEK 75 M6 708 102 MASS(ES) TO 722 104 HEAD WEEK 102 729 105 MASS(ES) TO 732 105 HEAD WEEK 102 M6 603 87 TO 617 89 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 624 90 TO 627 90 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 40 X 40MM 60 X 55MM 65 X 70MM 80 X 80MM 40 X 35MM 50 X 40MM 30 X 30MM 40 X 50 907 Covance 6329-212 3M T-6316.1 PAGE: 197 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 198 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91081 6 C91082 6 C91083 6 C91084 6 C91085 6 C91086 6 C91087 6 C91088 6 C91089 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 908 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 199 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91090 6 C91091 6 C91092 6 C91093 6 C91094 6 C91095 6 C91096 6 C91097 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15 , 22, 29, 36, 43, 50 1, 8, 15 , 22, 29, 36, 43, 50 1, 8, 15 , 22, 29, 36, 43, 50 36, 43 , 50 1, 8, 15 , 22, 29 1, 8, 15 , 22, 29, 36, 43, 50 1, 8, 15 , 22, 29, 36, 43, 50 1, 8, 15 , 22, 29, 36, 43, 50 1, 8, 15 , 22, 29, 36, 43, 50 909 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 200 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91098 6 C91099 D C91100 6 8 NORMAL NO REMARKABLE OBSERVATIONS 6 APPEARANCE DEHYDRATED BEHAVIOR HYPOACTIVE DISCHARGE ORAL RED IN COLOR EXCRETION FEW FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 38-39 38-39 38-39 38-39 38-39 1, 8, 15, 22, 29, 36, 38 1, 8, 15, 22, 29, 36, 43, 50 910 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER GROUP: M7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED 'C' - COMMENTS LISTED AT END OF 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 22, 29, 36, 43, 50 1, 8, 15 911 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 202 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91110 6 C91111 6 C91112 6 C91113 6 C91114 6 C91115 6 C91116 6 C91117 6 C91118 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 SKIN & PELAGE SORE/SCAB HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 15 1, 8, 22, 29, 36, 43, 50 912 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 203 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91119 6 C91120 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 APPEARANCE THIN SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 50 43, 50 1, 8, 15, 22, 29, 36 913 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 204 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91121 4 53 NORMAL NO REMARKABLE OBSERVATIONS C91122 4 53 NORMAL NO REMARKABLE OBSERVATIONS C91123 D 98 BEHAVIOR HYPOACTIVE RECUMBENT STERNAL EYES CLOUDY DISCHARGE EYE-RIGHT RESPIRATION LABORED SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 683 683 113 683 659, 666, 673,. 680 , 683 1, 8,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 120, 127, 134, 141 , 148, 155, 162,. 169, 176, 183, 190, 197, 204 211, 218, 225, 232 , 239, 246, 253, 260, 267, 274, 281,. 288, 295 914 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 205 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91123 D 98 NORMAL NO REMARKABLE OBSERVATIONS C91124 4 MASS(ES) AXILLARY REGION-LEFT WEEK 97 AXILLARY REGION-RIGHT WEEK 85 INGUINAL REGION-RIGHT WEEK 94 53 NORMAL NO REMARKABLE OBSERVATIONS C91125 M 96 APPEARANCE MALOCCLUSION THIN EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582 673, 680, 683 58 9C, 596C , 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 65 666C, 673C , 680C, 683C 652C, 659C , 666C, 673C, 680C, 683C 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 253 671 659, 666 671 671 915 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 206 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91125 M 96 RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS C91126 2 C91127 T MASS(ES) AXILLARY REGION-LEFT WEEK 75 AXILLARY REGION-RIGHT WEEK 76 INGUINAL REGION-RIGHT WEEK 87 8 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-LEFT DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 671 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 561, 568, 575 , 582, 589, 596 519, 526, 533,, 540 , 547, 554 526, 533, 540, 547 , 554 603C, 610C , 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 671C 1, 8,. 15, 22, 29, 36, 43, 50, 54 724, 727 724, 727, 729-730 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 724, 727, 729-730 916 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 207 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91127 T 105 SKIN & PELAGE SORE/SCAB LIMB-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C91128 M MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 75 EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568, 575, 582, 589 , 596, 603 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540, 547, 554 , 561 610 631, 638, 645, 652 , 659C , 666C , 673C, 680C, 687C, 694C , 701C 708C, 715C , 722C, 724C, 727C, 729-730C 523 523 523 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 917 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 208 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91128 M 75 NORMAL NO REMARKABLE OBSERVATIONS C91129 3 14 NORMAL NO REMARKABLE OBSERVATIONS C91130 D 77 NORMAL NO REMARKABLE OBSERVATIONS C91131 T 105 APPEARANCE THIN SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 94 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533- 534 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729-730 687, 694, 701, 708, 715, 722, 729-730 659, 666, 673, 680 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575 918 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 209 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91132 M 89 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 64 C91133 4 53 NORMAL NO REMARKABLE OBSERVATIONS C91134 M 75 APPEARANCE HUNCHED THIN EXCRETION FEW FECES 498, 505, 512, 519 , 526, 533, 540, 547, 554, 561 , 568, 575, 582 589, 596, 603, 610 , 617- 618 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85 , 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372, 379 386, 393, 400, 407 , 414, 421, 428, 435 442, 449, 456, 463 , 470C , 477C, 484C, 491C, 498C , 505C , 512C 519C , 526C , 533C, 54 0C, 547C, 554C , 561C, 568C, 575C, 582C, 589C 596C , 603C , 610C, 617-618C 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85 , 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 367 518- 519 491, 498, 501, 505 , 512, 518- 519 477, 484, 491, 498 , 501, 505, 512, 518-519 919 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 210 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91134 M 75 EYES RED DISCHARGE EYES SKIN & PELAGE COLD TO TOUCH ENTIRE BODY RED HAIR COAT LIMBS-FRONT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 59 AXILLARY REGION-RIGHT WEEK 56 VENTRAL-ABDOMINAL WEEK 51 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 518-519 518-519 505, 512, 518-519 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 407, 414, 421,. 428, 435, 442C,, 449C, 456C, 463C, 470C, 477C 484C, 491C 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491 351C, 358C , 365C, 372C, 379C, 386C , 393C, 400C, 407C, 414C, 421C 428C, 435C , 442C, 449C, 456C, 463C , 470C, 477C, 484C, 491C, 498C 501C, 505C , 512C, 518-519C 920 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 211 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91135 1 C91136 M 4 NORMAL NO REMARKABLE OBSERVATIONS 90 APPEARANCE HEAD TILT RIGHT EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91137 M 103 APPEARANCE LIMITED USE PAW-HIND-RIGHT SWOLLEN PAW-HIND-RIGHT 1, 8,, 15, 22, 24 624 610, 617, 624 568, 575, 582, 589, 596, 603, 610, 617, 624 624 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 701, 708, 714-715 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 714- 715 921 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 212 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91137 M 103 BEHAVIOR HYPOACTIVE EXCRETION NO FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 715 708, 714-715 708, 714-715 708, 714-715 708, 714-715 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610 922 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 213 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91138 T 105 APPEARANCE MALOCCLUSION C91139 M EXCRETION NONFORMED FECES SKIN & PELAGE RED HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 99 62 APPEARANCE HEAD TILT RIGHT EYES RED DISCHARGE EYE-RIGHT 176, 183, 19 0, 197 , 204, 211, 218, 225 , 232, 239, 246, 253 , 260 267, 274, 281, 288 , 295, 302, 309, 316 , 323, 330, 337, 344 , 351 358, 365, 372, 379 , 386, 393, 400, 407 , 414, 421, 428, 435 , 442 449, 456, 463, 470 , 477, 484, 491, 498 , 505, 512, 519, 526 , 533 540, 547, 554, 561 , 568, 575, 582, 589 , 596, 603, 610, 617 , 624 631, 638, 645, 652 , 659, 666, 673, 680 , 687, 694, 701, 708 , 715 722, 729, 732 568 533 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169 687C, 694C, 701C, 708C, 715C, 722C, 729C, 732C 432 421, 428, 432 923 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 214 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91139 M 62 NORMAL NO REMARKABLE OBSERVATIONS C91140 2 C91141 D 8 NORMAL NO REMARKABLE OBSERVATIONS 80 NORMAL NO REMARKABLE OBSERVATIONS C91142 M MASS(ES) LIMB-HIND-RIGHT WEEK 71 73 BEHAVIOR SENSITIVE TO TOUCH EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-LEFT DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407, 414 1, 8,, 15, 22, 29, 36, 43 , 50, 54 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,, 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484 491C, 498C , 505C, 512C, 519C, 526C , 533C , 540C, 547C, 554C , 558C 505 498, 505 505 351 924 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 215 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91142 M 73 EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91143 1 C91144 T MASS(ES) INGUINAL REGION-RIGHT WEEK 72 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL THIN DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 498, 505 505 505 1, 8, 15 113, 120 204, 211 295, 302 393, 400 484, 491 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 358, 365, 372, 379 , 386 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 498C, 505C 1, 8, 15, 22, 24 729, 731 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 T72O 2T 925 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 216 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91144 T 105 DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS C91145 2 C91146 M 8 NORMAL NO REMARKABLE OBSERVATIONS 75 APPEARANCE HEAD TILT LEFT MALOCCLUSION EYES RED DISCHARGE EYE-LEFT EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 729, 731 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582,. 589, 596, 603, 610, 617 , 624 1, 8,, 15, 22, 29, 36, 43,, 50, 54 509, 512, 519 253, 414, 421,. 428, 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 509,. 512, 519 509, 512, 519 519 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 260 , 267, 274, 281, 288 , 295 302, 309, 316,. 323, 330, 337, 344, 351 , 358, 365, 372, 379 , 386 926 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 217 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91146 M 75 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 71 C91147 M 77 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR HYPOACTIVE SENSITIVE TO TOUCH EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 393, 400, 407 491C, 498C, 505C, 509C, 512C, 519C 532-533, 535 535 532-533 532-533 532-533, 535 535 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 330, 337, 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421,. 428, 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519, 526 927 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 218 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91147 M 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 45 C91148 T 105 DISCHARGE GENITAL RED IN COLOR SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 102 INGUINAL REGION-RIGHT WEEK 98 VENTRAL-ABDOMINAL WEEK 95 VENTRAL-ABDOMINAL-RIGHT WEEK 98 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 309, 316, 323 568 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 701, 708, 715, 722 , 729- 730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 575 708C, 715C, 722C, 729-730C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 659, 666, 673, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729-730C 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 928 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 219 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91149 M 88 APPEARANCE THIN EYES PALE EYES SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT LIMB-FRONT-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 82 VENTRAL-ABDOMINAL-RIGHT WEEK 51 610 610 610 610 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78,, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 568C, 575C, 582C, 58 9C, 596C, 603C , 610C 351C, 358C, 365C, 372C, 379C, 386C , 393C , 400C, 407C, 414C , 421C 42 8C, 435C, 442C, 44 9C, 456C, 463C , 470C , 477C, 484C, 491C , 498C 505C, 512C, 519C, 526C, 533C, 540C , 547C , 554C, 561C, 568C , 575C 582C,. 589C, 596C, 603C, 610C 929 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 220 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91150 M 76 EYES PALE EYES RESPIRATION LABORED SKIN & PELAGE BLUE SKIN VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS C91151 M MASS(ES) VENTRAL-CERVICAL WEEK 62 74 APPEARANCE HEAD TILT LEFT THIN OTHER EXCRETION FEW FECES 526 526 498, 505, 512, 519, 526 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421 428C, 435C , 442C, 449C, 456C, 463C , 470C, 477C, 484C, 491C , 498C 505C, 512C , 519C, 526C 512 491, 498, 505, 512 498C 484, 491, 498, 505, 512 930 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 221 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91151 M 74 EYES RED DISCHARGE EYES HOUSING,FOOD,WATER & MAINT. WATER BOTTLE PLACED ON CAGE NORMAL NO REMARKABLE OBSERVATIONS C91152 4 MASS(ES) AXILLARY REGION-RIGHT WEEK 47 53 NORMAL NO REMARKABLE OBSERVATIONS C91153 M 81 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 512 491 1, 8,, 15, 22, 29, 36 , 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 379, 386, 393, 400, 407, 414, 421,. 428, 435 442, 449, 456, 463, 470, 477 323C, 330C , 337, 344 , 351 , 358 , 365, 372 1, 8,, 15, 22, 29, 36 , 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337,. 344, 351, 358, 365, 367 561 554, 561 1, 8,, 15, 22, 29, 36 , 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 931 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 222 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91153 M 81 NORMAL NO REMARKABLE OBSERVATIONS C91154 D MASS(ES) AXILLARY REGION-RIGHT WEEK 60 78 EXCRETION NONFORMED FECES EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 414C, 421C , 428C, 435C, 442C, 449C , 456C , 463C, 470C, 477C , 484C 491C, 498C , 505C, 512C, 519C, 526C , 533C , 540C, 547C, 554C , 561C 204 393, 400, 407,. 414, 421, 428, 435, 442, 449, 456 , 463, 470 , 477 484, 491, 498,. 505, 512, 519, 526, 533, 540- 541 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78 , 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 211, 218, 225,. 232, 239, 246, 253, 260, 267, 274 , 281, 288 , 295 302, 309, 316,. 323, 330, 337, 344, 351, 358, 365 , 372, 379 , 386 932 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 223 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91155 3 C91156 M 14 NORMAL NO REMARKABLE OBSERVATIONS 75 APPEARANCE MALOCCLUSION DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES PALE EYES SKIN & PELAGE RED HAIR COAT PERINEAL AREA SORE/SCAB PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 169, 176, 225 169 519 519 512, 519 463, 470 477, 484 , 491, 498, 505, 512 , 519 519 1, 8, 15 113, 120 218, 232 316, 323 449, 456 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 183, 190, 197, 204 , 211 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302 , 309 330, 337 , 344, 351, 358, 365 , 372, 379, 428, 435 , 442 933 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 224 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91156 M 75 MASS(ES) AXILLARY REGION-LEFT WEEK 56 C91157 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91158 M 94 DISCHARGE GENITAL RED IN COLOR SKIN & PELAGE SORE/SCAB LIMBS-HIND PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 400 596, 603, 610 617, 624, 631, 638 , 645, 652 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 407, 414 , 421, 428, 435, 442 , 449, 456, 463 934 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 225 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91158 M 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 C91159 T 105 EYES CLEAR DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS C91160 3 MASS(ES) AXILLARY REGION-LEFT WEEK 85 14 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C 659, 666 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582 589, 596, 603,. 610, 617, 624, 631C , 638C, 645C, 652C, 659C , 666C 673C, 680C', 687C, 694C, 701C, 708C , 715C, 722C, 729-730C 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 94 935 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 226 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91161 M 61 APPEARANCE HEAD TILT RIGHT THIN EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C91162 2 WART LIKE LESION(S) MOUTH WEEK 21 8 NORMAL NO REMARKABLE OBSERVATIONS 421 414, 421 400, 414, 421 393, 400, 407, 414 , 421 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 183, 190, 197, 204, 211, 218, 225,. 232, 239 246, 253, 260, 267 , 274, 281, 288, 295, 302, 309, 316,. 323, 330 337, 344, 351, 358 , 365, 372, 379, 386 141, 148, 155, 162, 169, 176 1, 8, 15, 22, 29, 36, 43, 50, 54 936 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 227 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91163 2 C91164 M 8 NORMAL NO REMARKABLE OBSERVATIONS 95 APPEARANCE HUNCHED EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 86 1, 8, 15, 22, 29, 36, 43, 50, 54 659 659 652, 659 631, 638, 645, 652, 659 652, 659 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183 , 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365 , 372 , 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 , 554 , 561 568, 575, 582, 589 596, 603C, 610C, 617C, 624C, 631C, 638C, 645C , 652C, 659C 937 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 228 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91165 1 C91166 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 NORMAL NO REMARKABLE OBSERVATIONS C91167 D MASS(ES) HEAD WEEK 86 103 APPEARANCE LIMITED USE PAW-HIND-LEFT SWOLLEN PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694, 701, 708, 715, 722 , 729, 731 596, 603 701, 708, 715, 717 463, 470, 477, 484 , 491, 498, 505, 512 , 519, 526, 533, 540 , 547 554, 561, 568, 575 , 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 659, 666 , 673, 680, 687, 694 , 701, 708, 715, 717 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456 938 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 229 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER (CONTINUED FROM PREVIOUS PAGE) C91167 D 103 MASS(ES) DORSAL WEEK 82 GROUP: F1 C91168 M 84 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR SENSITIVE TO TOUCH EXCRETION FEW FECES NO FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568, 575, 582, 589, 596, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C n71i7n rC' 588 582, 588 579, 582, 588 582 588 579, 582, 588 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575 939 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 230 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91169 1 C91170 M 4 NORMAL NO REMARKABLE OBSERVATIONS 89 APPEARANCE HEAD TILT RIGHT HUNCHED MALOCCLUSION SWOLLEN MOUTH THIN EXCRETION FEW FECES SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) MOUTH WEEK 71 1, 8, 15, 22, 24 603, 610, 617-618 617-618 540, 547, 551, 554, 561, 568, 575, 582, 589, 596, 603, 610 617-618 551, 554, 561 603, 610, 617-618 551, 554, 561, 596, 603, 610, 617-618 617-618 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 533 491, 498, 505, 512 , 519, 526 940 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 231 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91171 1 C91172 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION MISSING TEETH SKIN & PELAGE SORE/SCAB MOUTH PAWS-HIND YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 87 1, 8, 15, 22, 24 141, 148, 155, 162 , 169, 176, 183, 190 , 197, 204, 211, 218 , 225 232, 239, 246, 253 , 260, 267, 274, 281 , 288, 295, 302, 309 , 316 323, 330, 337, 344 , 351, 358, 365, 372 , 379, 386, 393, 400 , 407 414, 421, 428, 435 , 442, 449, 456, 463 , 470, 477, 484, 491 , 498 505, 512, 519, 526 , 533, 540, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617 , 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701, 708 , 715, 722, 729- 730 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 722, 729- 730 582 617, 624, 631, 638 , 645, 652, 659, 666 , 673, 680, 687, 694 , 701 708, 715, 722, 729 -730 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610, 617 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 603,610 941 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 232 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91173 M 58 APPEARANCE THIN BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 393, 400, 404 404 393, 400, 404 393, 404 400, 404 400, 404 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386 942 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 233 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91174 3 C91175 M 14 NORMAL NO REMARKABLE OBSERVATIONS 86 APPEARANCE HEAD TILT LEFT BEHAVIOR ATAXIC EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS ASS(ES) AXILLARY REGION-LEFT WEEK 74 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 596, 600 600 600 1, 8,, 15, 22, 29, 36, 43,r 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183 , 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274 , 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358, 365 , 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449, 456 , 463 , 470 477, 484, 491, 498 , 505 512, 519, 526, 533 , 540, 547, 554, 561C, 568C , 575C, 582C, 589C 596C, 600C 943 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 234 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91176 T 105 APPEARANCE LIMITED USE PAW-HIND-LEFT SWOLLEN PAW-HIND-LEFT SKIN & PELAGE SORE/SCAB LIMB-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91177 M 94 APPEARANCE THIN NORMAL NO REMARKABLE OBSERVATIONS 701, 708, 715, 722, 729-730 674, 680, 687, 694, 701, 708, 715, 722, 729-730 589 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 596 , 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 640, 645, 652 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428 944 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 235 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91177 M 94 MASS(ES) AXILLARY REGION-LEFT WEEK 63 C91178 M 91 APPEARANCE HEAD TILT LEFT MALOCCLUSION BEHAVIOR ATAXIC HYPOACTIVE EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 65 AXILLARY REGION-RIGHT WEEK 67 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 512C, 519C, 526C, 533C, 54 0C, 547C, 554C, 561C, 568C, 575C, 582C 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 640C, 645C, 652C 631 372, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 631 631 617, 624, 631 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 379 , 386 393, 400, 407,. 414, 421, 428, 435, 442 , 491, 498, 505, 512 , 519 526, 533, 540,. 547, 554, 561 44 9C, 456C , 463C, 470C, 477C, 484C 463, 470, 477, 484 945 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 236 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91178 M 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 WART LIKE LESION(S) HEAD-MAXILLARY-LEFT WEEK 89 C91179 M 47 APPEARANCE THIN SKIN & PELAGE COLD TO TOUCH ENTIRE BODY PALE ENTIRE BODY SORE/SCAB VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 38 VENTRAL-ABDOMINAL-RIGHT WEEK 36 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 624C, 631C 617 324 323 323-324 323-324 1, 8,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78,. 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239 260, 267, 274C , 281C, 288C, 295C, 302C , 309C, 316C, 323-324C 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C 323-324C 946 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 237 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91180 D 64 SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 26 AXILLARY REGION-RIGHT WEEK 38 LIMB-HIND-LEFT WEEK 25 VENTRAL-ABDOMINAL-RIGHT WEEK 32 C91181 3 C91182 M 14 NORMAL NO REMARKABLE OBSERVATIONS 88 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES 15, 22 1, 8, 29, 36, 43, 50, 57,. 64, 71, 78, 85, 92,, 99, 106, 113 127, 134, 141,, 148, 155, 162, 19 0, 197, 204, 211 176, 183 260, 267, 274, 281, 288 169 218C, 225C, 232C, 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 446C 1, 8, 15, 22 , 29, 36, 43 , 50, 57, 64 , 71, 78, 85 , 92, 94 589, 596, 603, 610 440, 610 440, 589, 596, 603, 610 947 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 238 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91182 M 88 EYES RED DISCHARGE EYES RESPIRATION LABORED SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91183 T MASS(ES) INGUINAL REGION-LEFT WEEK 87 105 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 440, 561, 568, 575, 582, 589, 596, 603, 610 440 582, 589, 596, 603, 610 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 603C, 610C 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 435, 442 , 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610, 617, 624 , 631, 638, 645, 652 , 659, 666, 673, 680 , 687 694, 701, 708, 715 , 722, 729- 730 948 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 239 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91183 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 58 C91184 M 90 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL BEHAVIOR RECUMBENT STERNAL EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES RESPIRATION LABORED SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 400, 407, 414, 421, 428 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 629 617, 624 629 629 629 456, 463 470, 477 , 484 582, 589 596, 603 , 610, 617, 624, 629 624, 629 1, 8, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 949 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 240 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91184 M 90 NORMAL NO REMARKABLE OBSERVATIONS M 67 APPEARANCE HUNCHED THIN EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 491, 498 , 505 442, 449, 456, 463-464 449, 456, 463- 464 464 442, 449, 456, 463-464 456, 463- 464 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 950 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 241 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91186 M 98 APPEARANCE THIN SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 76 C91187 M 73 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN BEHAVIOR ATAXIC HYPOACTIVE 631, 638, 645, 652, 659, 666, 673, 680 680 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78,r 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519 526C, 533C , 540C, 547C, 554C, 561C , 568C, 575C, 582C, 589C , 596C 603C, 610C , 617C, 624C, 631C, 638C , 645C, 652C, 659C, 666C , 673C 680C 508- 509 491, 498, 501, 505 435, 442, 449, 463 491, 498, 501, 505 508-509 508-509 508- 509 509 951 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 242 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91187 M 73 EXCRETION FEW FECES NO FECES NONFORMED FECES EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91188 T MASS(ES) AXILLARY REGION-LEFT WEEK 71 105 APPEARANCE SWOLLEN INGUINAL REGION-LEFT INGUINAL REGION-RIGHT SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 498, 501, 505, 508 509 197, 491 508-509 1, 8,, 15, 22, 29, 36, 43 , 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148 , 155, 162 , 169, 176, 183,. 190, 204 211, 218, 225,. 232, 239, 246 , 253, 260 , 267, 274, 281,. 288, 295 302, 309, 316,. 323, 330, 337 , 344, 351 , 358, 365, 372, 379, 386 393, 400, 407,. 414, 421, 428 , 456, 470 , 477, 484 491C, 498C , 501C, 505C, 508- 509C 631, 638, 645, 652 631, 638, 645, 652 582, 589, 596, 603, 610 1, 8,, 15, 22, 29, 36, 43 , 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148 , 155, 162 , 169, 176, 183,. 190, 197 952 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 243 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91188 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91189 M MASS(ES) AXILLARY REGION-LEFT WEEK 74 VENTRAL -CERVICAL-LEFT WEEK 88 VENTRAL -THORACIC-LEFT WEEK 101 93 EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT LIMBS-HIND PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505 512, 519, 526C, 53 3C, 54 0C, 547C, 554C, 561C, 568C, 575C, 582C 58 9C, 596C , 603C, 610C , 617C , 624C , 631C , 638C , 645C , 652C , 659C 666C, 673C , 680C, 687C , 694C , 701C , 708C , 715C , 722C , 72 9C , 731C 610C, 617C , 624C, 631C , 638C , 645C , 652C , 659C , 666C , 673C , 680C 687C, 694C , 701C, 708C , 715C , 722C , 72 9C , 731C 701C, 708C , 715C, 722C , 72 9C , 731C 645 645 603, 610, 617,. 624, 631, 638, 645 554, 561, 568,. 575, 582, 589, 596 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 953 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 244 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F1 (CONTINUED FROM PREVIOUS PAGE) C91189 M 93 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 88 INGUINAL REGION-RIGHT WEEK 76 C91190 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 100 DOSE: 0 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 610C, 617C, 624C, 631C, 638C, 645C 526, 533, 540, 547C, 554C, 561C, 568, 575, 582, 589, 596 589, 596, 603, 610 , 645, 652, 659, 666, 673, 680, 687, 694 , 701 708, 715, 722,. 729 , 731 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540, 547, 554 , 561 568, 575, 582, 617 , 624, 631, 638 694C, 701C , 708C, 715C, 722C, 72 9C , 731C 954 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 88 MASS(ES) 93 AXILLARY REGION-RIGHT WEEK 85 94 MASS(ES) 96 AXILLARY REGION-RIGHT WEEK 85 97 MASS(ES) 98 AXILLARY REGION-RIGHT WEEK 85 94 MASS(ES) INGUINAL REGION-RIGHT WEEK 94 95 MASS(ES) INGUINAL REGION-RIGHT WEEK 94 20 X 20MM 30 X 25MM 35 X 30MM 35 X 40MM 40 X 50MM 50 X 60MM 15 X 15MM 20 X 30MM 955 Covance 6329-212 3M T-6316.1 PAGE: 245 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 94 97 MASS(ES) 98 INGUINAL REGION-RIGHT WEEK 94 87 MASS(ES) 90 INGUINAL REGION-RIGHT WEEK 87 91 MASS(ES) 93 INGUINAL REGION-RIGHT WEEK 87 94 MASS(ES) INGUINAL REGION-RIGHT WEEK 87 95 MASS(ES) 96 INGUINAL REGION-RIGHT WEEK 87 96 MASS(ES) 96 INGUINAL REGION-RIGHT WEEK 87 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 30 X 30MM 40 X 40MM 35 X 35MM 45 X 35MM 80 X 40MM 60 X 40MM 70 X 55MM 25 X 30MM 956 Covance 6329-212 3M T-6316.1 PAGE: 246 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91127 C91132 F1 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 91 673 97 MASS(ES) TO 701 101 VENTRAL-ABDOMINAL-RIGHT WEEK 91 708 102 MASS(ES) TO 730 105 VENTRAL-ABDOMINAL-RIGHT WEEK 91 F1 470 68 TO 484 70 MASS(ES) INGUINAL REGION-LEFT WEEK 64 491 71 MASS(ES) INGUINAL REGION-LEFT WEEK 64 498 72 TO 561 81 MASS(ES) INGUINAL REGION-LEFT WEEK 64 568 82 TO 582 84 MASS(ES) INGUINAL REGION-LEFT WEEK 64 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 64 30 X 30MM 40 X 40MM 60 X 50MM 15 X 15MM 30 X 30MM 70 X 50MM 80 X 65MM 90 X 80MM 957 Covance 6329-212 3M T-6316.1 PAGE: 247 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 86 MASS(ES) INGUINAL REGION-LEFT WEEK 64 87 MASS(ES) INGUINAL REGION-LEFT WEEK 64 88 MASS(ES) INGUINAL REGION-LEFT WEEK 64 89 MASS(ES) 89 INGUINAL REGION-LEFT WEEK 64 64 MASS(ES) 71 AXILLARY REGION-LEFT WEEK 59 51 MASS(ES) 59 VENTRAL-ABDOMINAL WEEK 51 60 MASS(ES) 61 VENTRAL-ABDOMINAL WEEK 51 62 MASS(ES) 64 VENTRAL-ABDOMINAL WEEK 51 100 X 90MM 110 X 90MM 110 X 120MM 80 X 90MM 10 X 10MM 10 X 10MM 20 X 20MM 30 X 30MM 958 Covance 6329-212 3M T-6316.1 PAGE: 248 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91134 C91138 C91142 F1 449 65 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 470 68 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 F1 687 99 MASS(ES) TO 694 100 AXILLARY REGION-LEFT WEEK 99 701 101 MASS(ES) AXILLARY REGION-LEFT WEEK 99 708 102 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 99 F1 491 71 TO 519 75 MASS(ES) LIMB-HIND-RIGHT WEEK 71 526 76 TO 558 80 MASS(ES) LIMB-HIND-RIGHT WEEK 71 F1 498 72 TO 505 73 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 35 X 40MM 60 X 40MM 50 X 40MM 60 X 50MM 50 X 70MM 15 X 15MM 20 X 30MM 15 X 15MM 959 Covance 6329-212 3M T-6316.1 PAGE: 249 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 71 MASS(ES) 75 AXILLARY REGION-RIGHT WEEK 71 102 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 102 98 MASS(ES) 99 INGUINAL REGION-RIGHT WEEK 98 100 MASS(ES) 105 INGUINAL REGION-RIGHT WEEK 98 98 MASS(ES) 101 VENTRAL-ABDOMINAL WEEK 95 102 MASS(ES) 105 VENTRAL-ABDOMINAL WEEK 95 98 MASS(ES) 101 VENTRAL-ABDOMINAL-RIGHT WEEK 98 102 MASS(ES) 105 VENTRAL-ABDOMINAL-RIGHT WEEK 98 10 X 15MM 50 X 45MM 40 X 30MM 40 X 50MM 20 X 20MM 20 X 20MM 20 X 20MM 30 X 25MM 960 Covance 6329-212 3M T-6316.1 PAGE: 250 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 82 MASS(ES) 84 AXILLARY REGION-LEFT WEEK 82 85 MASS(ES) AXILLARY REGION-LEFT WEEK 82 86 MASS(ES) 88 AXILLARY REGION-LEFT WEEK 82 51 MASS(ES) 53 VENTRAL-ABDOMINAL-RIGHT WEEK 51 54 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 55 MASS(ES) 56 VENTRAL-ABDOMINAL-RIGHT WEEK 51 57 MASS(ES) 59 VENTRAL-ABDOMINAL-RIGHT WEEK 51 60 MASS(ES) 61 VENTRAL-ABDOMINAL-RIGHT WEEK 51 20 X 20MM 25 X 25MM 35 X 30MM 20 X 10MM 30 X 20MM 35 X 25MM 35 X 30MM 40X35MM 961 Covance 6329-212 3M T-6316.1 PAGE: 251 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 62 MASS(ES) 63 VENTRAL-ABDOMINAL-RIGHT WEEK 51 64 MASS(ES) 81 VENTRAL-ABDOMINAL-RIGHT WEEK 51 82 MASS(ES) 86 VENTRAL-ABDOMINAL-RIGHT WEEK 51 87 MASS(ES) 88 VENTRAL-ABDOMINAL-RIGHT WEEK 51 62 MASS(ES) 63 VENTRAL-CERVICAL WEEK 62 64 MASS(ES) VENTRAL-CERVICAL WEEK 62 65 MASS(ES) 67 VENTRAL-CERVICAL WEEK 62 68 MASS(ES) 71 VENTRAL-CERVICAL WEEK 62 40 X 40MM 45 X 40MM 50 X 50MM 50 X 55MM 30 X 30MM 35 X 35MM 40 X 40MM 45 X 45MM 962 Covance 6329-212 3M T-6316.1 PAGE: 252 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91150 C91151 C91153 C91158 F1 498 72 TO 526 76 F1 498 72 323 47 TO 330 48 F1 414 60 TO 484 70 491 71 TO 533 77 540 78 TO 561 81 F1 470 68 TO 484 70 491 71 TO 533 77 MASS(ES) VENTRAL-CERVICAL WEEK 62 APPEARANCE OTHER MASS(ES) AXILLARY REGION-RIGHT WEEK 47 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 45 X 60MM GENITAL DISCHARGE, GREEN 10 X 10MM 10 X 10MM 20 X 20MM 40 X 25MM 10 X 10MM 20 X 20MM 963 Covance 6329-212 3M T-6316.1 PAGE: 253 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 78 MASS(ES) 81 AXILLARY REGION-RIGHT WEEK 68 82 MASS(ES) 84 AXILLARY REGION-RIGHT WEEK 68 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 88 MASS(ES) 90 AXILLARY REGION-RIGHT WEEK 68 91 MASS(ES) 93 AXILLARY REGION-RIGHT WEEK 68 94 MASS(ES) 94 AXILLARY REGION-RIGHT WEEK 68 35 X 30MM 45 X 50MM 50 X 55MM 60 X 55MM 65 X 60MM 90 X 75MM 100 X 80MM 110 X 90MM 964 Covance 6329-212 3M T-6316.1 PAGE: 254 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91158 C91159 C91164 C91167 F1 652 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 68 F1 631 91 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 85 659 95 MASS(ES) TO 701 101 AXILLARY REGION-LEFT WEEK 85 708 102 MASS(ES) TO 730 105 AXILLARY REGION-LEFT WEEK 85 F1 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 86 610 88 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 86 F1 603 87 MASS(ES) DORSAL WEEK 82 610 88 MASS(ES) DORSAL WEEK 82 110 X 90MM 10 X 10MM 15 X 15MM 20 X 20MM 20 X 20MM 20 X 25MM 15 X 20MM 20 X 20MM 965 Covance 6329-212 3M T-6316.1 PAGE: 255 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 89 MASS(ES) DORSAL WEEK 82 90 MASS(ES) DORSAL WEEK 82 91 MASS(ES) DORSAL WEEK 82 92 MASS(ES) DORSAL WEEK 82 93 MASS(ES) DORSAL WEEK 82 94 MASS(ES) DORSAL WEEK 82 95 MASS(ES) DORSAL WEEK 82 96 MASS(ES) DORSAL WEEK 82 20 X 20MM 20 X 20MM 20 X 20MM 20 X 20MM 20 X 20MM 20 X 30MM 20 X 30MM 20 X 30MM 966 Covance 6329-212 3M T-6316.1 PAGE: 256 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91167 F1 673 97 MASS(ES) DORSAL WEEK 82 680 98 MASS(ES) DORSAL WEEK 82 687 99 MASS(ES) DORSAL WEEK 82 694 100 MASS(ES) DORSAL WEEK 82 701 101 MASS(ES) DORSAL WEEK 82 708 102 MASS(ES) DORSAL WEEK 82 715 103 MASS(ES) DORSAL WEEK 82 717 103 MASS(ES) DORSAL WEEK 82 20 X 30MM 35 X 45MM 40 X 45MM 40 X 45MM 40 X 45MM 40 X 45MM 40 X 45MM 40 X 45MM 967 Covance 6329-212 3M T-6316.1 PAGE: 257 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 81 MASS(ES) 84 AXILLARY REGION-LEFT WEEK 74 85 MASS(ES) AXILLARY REGION-LEFT WEEK 74 86 MASS(ES) 86 AXILLARY REGION-LEFT WEEK 74 63 MASS(ES) AXILLARY REGION-LEFT WEEK 63 64 MASS(ES) AXILLARY REGION-LEFT WEEK 63 65 MASS(ES) 67 AXILLARY REGION-LEFT WEEK 63 68 MASS(ES) 71 AXILLARY REGION-LEFT WEEK 63 72 MASS(ES) 75 AXILLARY REGION-LEFT WEEK 63 35 X 30MM 45 X 40MM 50 X 45MM 30MM X 40MM 50 X 45MM 60 X 65MM 65 X 70MM 80 X 70MM 968 Covance 6329-212 3M T-6316.1 PAGE: 258 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 76 MASS(ES) 78 AXILLARY REGION-LEFT WEEK 63 79 MASS(ES) 81 AXILLARY REGION-LEFT WEEK 63 82 MASS(ES) 85 AXILLARY REGION-LEFT WEEK 63 86 MASS(ES) 87 AXILLARY REGION-LEFT WEEK 63 88 MASS(ES) 94 AXILLARY REGION-LEFT WEEK 63 65 MASS(ES) 70 AXILLARY REGION-LEFT WEEK 65 90 MASS(ES) 91 VENTRAL-ABDOMINAL WEEK 90 40 MASS(ES) 42 AXILLARY REGION-RIGHT WEEK 38 90 X 70MM 90 X 80MM 90 X 100MM 100 X 110MM 130 X 110MM 10 X 10MM 10 X 10MM 10 X 10MM 969 Covance 6329-212 3M T-6316.1 PAGE: 259 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 43 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 44 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 45 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 46 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 47 MASS(ES) 47 AXILLARY REGION-RIGHT WEEK 38 36 MASS(ES) 39 VENTRAL-ABDOMINAL-RIGHT WEEK 36 40 MASS(ES) 43 VENTRAL-ABDOMINAL-RIGHT WEEK 36 44 MASS(ES) 45 VENTRAL-ABDOMINAL-RIGHT WEEK 36 20 X 20MM 30 X 30MM 45 X 55MM 65 X 60MM 80 X 65MM 25 X 20MM 30 X 25MM 50 X 40MM 970 Covance 6329-212 3M T-6316.1 PAGE: 260 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 46 MASS(ES) 47 VENTRAL-ABDOMINAL-RIGHT WEEK 36 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 33 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 34 MASS(ES) 35 VENTRAL-ABDOMINAL-RIGHT WEEK 32 36 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 37 MASS(ES) 38 VENTRAL-ABDOMINAL-RIGHT WEEK 32 39 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 40 MASS(ES) 44 VENTRAL-ABDOMINAL-RIGHT WEEK 32 65 X 50MM 15 X 15MM 20 X 30MM 30 X 30MM 45 X 40MM 50 X 55MM 60 X 55MM 70 X 65MM 971 Covance 6329-212 3M T-6316.1 PAGE: 261 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91180 C91182 F1 309 45 316 46 323 47 330 48 TO 344 50 351 51 TO 421 61 428 62 TO 435 63 442 64 TO 446 64 F1 603 87 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 32 MASS(ES) INGUINAL REGION-LEFT WEEK 87 80 X 80MM 90 X 80MM 110 X 90MM 110 X 95MM 130 X 110MM 145 X 115MM 150 X 120MM 10 X 15MM 972 Covance 6329-212 3M T-6316.1 PAGE: 262 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 76 MASS(ES) 81 INGUINAL REGION-LEFT WEEK 76 82 MASS(ES) 83 INGUINAL REGION-LEFT WEEK 76 84 MASS(ES) 85 INGUINAL REGION-LEFT WEEK 76 86 MASS(ES) INGUINAL REGION-LEFT WEEK 76 87 MASS(ES) INGUINAL REGION-LEFT WEEK 76 88 MASS(ES) 94 INGUINAL REGION-LEFT WEEK 76 95 MASS(ES) 97 INGUINAL REGION-LEFT WEEK 76 98 MASS(ES) 98 INGUINAL REGION-LEFT WEEK 76 20 X 20MM 30 X 30MM 50 X 40MM 60 X 55MM 80 X 60MM 80 X 75MM 80 X 80MM 90 X 80MM 973 Covance 6329-212 3M T-6316.1 PAGE: 263 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 98 MASS(ES) INGUINAL REGION-LEFT WEEK 76 71 MASS(ES) 72 AXILLARY REGION-LEFT WEEK 71 73 MASS(ES) AXILLARY REGION-LEFT WEEK 71 73 MASS(ES) 73 AXILLARY REGION-LEFT WEEK 71 76 MASS(ES) 81 AXILLARY REGION-LEFT WEEK 74 82 MASS(ES) AXILLARY REGION-LEFT WEEK 74 83 MASS(ES) 85 AXILLARY REGION-LEFT WEEK 74 86 MASS(ES) AXILLARY REGION-LEFT WEEK 74 90 X 80MM 15 X 15MM 20 X 20MM 25 X 25MM 20 X 15MM 20 X 20MM 30 X 30MM 35 X 35MM 974 Covance 6329-212 3M T-6316.1 PAGE: 264 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91188 F1 603 87 TO 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 74 645 93 TO 652 94 MASS(ES) AXILLARY REGION-LEFT WEEK 74 659 95 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 74 673 97 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 74 687 99 MASS(ES) TO 701 101 AXILLARY REGION-LEFT WEEK 74 708 102 MASS(ES) TO 722 104 AXILLARY REGION-LEFT WEEK 74 729 105 MASS(ES) AXILLARY REGION-LEFT WEEK 74 731 105 MASS(ES) AXILLARY REGION-LEFT WEEK 74 40 X 40MM 45 X 45MM 45 X 55MM 60 X 55MM 70 X 55MM 30 X 30MM 10 X 10MM 10 X 10MM 975 Covance 6329-212 3M T-6316.1 PAGE: 265 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 88 MASS(ES) 92 VENTRAL-CERVICAL-LEFT WEEK 88 93 MASS(ES) 96 VENTRAL-CERVICAL-LEFT WEEK 88 97 MASS(ES) 101 VENTRAL-CERVICAL-LEFT WEEK 88 102 MASS(ES) 105 VENTRAL-CERVICAL-LEFT WEEK 88 101 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 101 102 MASS(ES) 105 VENTRAL-THORACIC-LEFT WEEK 101 88 MASS(ES) AXILLARY REGION-RIGHT WEEK 88 89 MASS(ES) 93 AXILLARY REGION-RIGHT WEEK 88 30 X 30MM 45 X 45MM 45 X 60MM 65 X 70MM 10 X 10MM 60 X 55MM 20 X 20MM 30 X 30MM 976 Covance 6329-212 3M T-6316.1 PAGE: 266 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91189 C91190 F1 547 79 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 76 F1 694 100 MASS(ES) TO 701 101 VENTRAL-CERVICAL WEEK 100 708 102 MASS(ES) TO 731 105 VENTRAL-CERVICAL WEEK 100 15 X 15MM 25 X 25MM 50 X 45MM Covance 6329-212 3M T-6316.1 PAGE: 267 977 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 268 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91191 D 98 APPEARANCE MALOCCLUSION SWOLLEN LIMB-HIND-LEFT MOUTH PAW-FRONT-LEFT PAW-HIND-RIGHT SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB PAW-HIND-LEFT PAWS-HIND VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 92 INGUINAL REGION-RIGHT WEEK 76 WEEK 83 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 582 435, 442, 449 407, 414, 421, 428 344, 351, 358 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 29 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 1, 8,, 15, 22, 36, 43, 50,, 57, 64, 71, 78, 85,. 92, 99, 106, 113 120, 127, 134,. 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225,. 232, 239, 246, 253, 260, 267 638C, 645C, 652C, 659C, 666C, 673C, 680C 526C, 533C 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645 652C, 659C, 666C, 673C, 680C 978 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 269 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91191 D 98 MASS(ES) MOUTH WEEK 85 C91192 3 14 NORMAL NO REMARKABLE OBSERVATIONS C91193 M 91 APPEARANCE HEAD TILT RIGHT BEHAVIOR CIRCLING RIGHT DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES RESPIRATION IRREGULAR SKIN & PELAGE RED HAIR COAT PERINEAL AREA SORE/SCAB DORSAL-CERVICAL PAW-HIND-RIGHT DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 589 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 629 629, 631 631 617, 624, 631 629 629 631 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 629, 631 979 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 270 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91193 M 91 SKIN & PELAGE SORE/SCAB PAWS-FRONT NORMAL NO REMARKABLE OBSERVATIONS C91194 M MASS(ES) AXILLARY REGION-LEFT WEEK 85 104 APPEARANCE MALOCCLUSION THIN BEHAVIOR RECUMBENT STERNAL EXCRETION FEW FECES DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 29, 36, 43, 50, 57, 64 1, 8,r 15, 22, 71, 78, 85,, 92, 99, 106, 113, 120, 127, 134, 141 148, 155, 162, 169 , 176, 183, 190, 197, 204, 211,. 218, 225, 232 239, 246, 253, 260 , 267, 274, 281,. 288, 295, 302, 309,. 316, 323 330, 337, 344, 351 , 358, 365, 372, 379,. 386, 393, 400, 407, 414 421, 428, 435, 442 , 449, 456, 463,. 470, 477,. 484, 491, 498, 505 512, 519 589, 596C, 603C , 610C, 617C, 624C, 629C , 631C 393, 400, 407, 414, 421, 428, 435,. 442, 449,. 456, 463, 470, 477 484, 491, 498, 505, 512, 519,, 526,. 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617,. 624, 631,. 638, 645, 652, 673 680, 687, 694, 701- 702, 708, 715, 722, 728 673, 680, 687, 694, 701-702, 708, 715, 722, 728 728 702, 708, 715, 722, 728 980 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 271 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91194 M 104 EYES RED DISCHARGE EYE-LEFT RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 71 M 100 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204 728 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78,r 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183,. 190, 197 211, 218, 225,. 232, 239, 246, 253, 260, 267, 274, 281,. 288, 295 302, 309, 316,. 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 491C, 498C , 505C, 512C, 519C, 526C , 533C, 540C, 547C, 554C, 561C 568C, 575C , 582C, 589C, 596C, 603C , 610C, 617C, 624C, 631C, 638C 645C, 652C , 659C, 666C, 673C, 680C , 687C, 694C, 701-702C, 708C 715C, 722C , 728C 659, 666, 673,. 676, 680, 683, 687, 691, 694 673, 676, 680,. 683, 687, 691, 694 691, 694 673, 676, 680,. 683, 687, 691, 694 981 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 272 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91195 M 100 EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91196 M MASS(ES) AXILLARY REGION-RIGHT WEEK 87 83 APPEARANCE THIN BEHAVIOR ATAXIC HYPOACTIVE EXCRETION FEW FECES NO FECES EYES PALE EYES DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 610, 617 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 676C, 680C, 683C, 687C, 691C, 694C 579 579 579 565, 575 568, 579 565, 568 982 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 273 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91196 M 83 EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL PALE EARS RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 80 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575, 579 565, 568, 575, 579 579 565, 568, 575, 579 579 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 554C, 561C, 565C, 568C, 575C, 579C 983 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 274 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91197 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91198 1 C91199 M 4 NORMAL NO REMARKABLE OBSERVATIONS 97 APPEARANCE HEAD TILT RIGHT BEHAVIOR ATAXIC CIRCLING LEFT EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 1, 8, 15, 22, 24 673 673 673 652, 659, 666, 673 652 652, 659, 666, 673 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 984 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 275 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91199 M 97 NORMAL NO REMARKABLE OBSERVATIONS C91200 M 87 BEHAVIOR SENSITIVE TO TOUCH SKIN & PELAGE SORE/SCAB HEAD-CRANIAL SHOULDER-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD WEEK 82 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 568, 575, 582, 589, 596, 603, 607 603, 607 29 1, 8,r 15, 22, 36, 43 , 50,r 57, 64, 71, 78, 85,r 92, 99, 106, 113 120, 127, 134 141, 148, 155, 162, 169 , 176, 183, 190, 197, 204 211, 218, 225 232, 239, 246, 253, 260 , 267, 274, 281,. 288, 295 302, 309, 316 323, 330, 337, 344, 351 , 358, 365, 372, 379, 386 393, 400, 407 414, 421, 428, 435, 442 , 449, 456, 463,. 470, 477 484, 491, 498 505, 512, 519, 526, 533 , 540, 547, 554, 561 568 575C, 582C, 589C, 596C, 603C 607C 985 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 276 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91201 T 105 EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 74 INGUINAL REGION-RIGHT WEEK 97 VENTRAL-ABDOMINAL-RIGHT WEEK 49 WEEK 105 WART LIKE LESION(S) MOUTH WEEK 52 715, 722, 729-730 673, 680, 687,. 694 , 701, 708, 715, 722 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 386, 393 , 400, 407, 414, 421 , 428 435, 442, 449, 456 , 463, 470, 477, 484 , 491, 498, 505 512, 519, 526, 533 , 540 , 547 , 554, 561, 568, 575 , 582C, 589C 596C , 603C , 610C, 617C, 624C , 631C, 638C , 645C, 652C, 659C, 666C 673C , 680C , 687C, 694C, 701C , 708C, 715C , 722C, 729-730C 673C , 680C , 687C, 694C, 701C , 708C, 715C , 722C, 729-730C 337C , 344C , 351C, 358, 365, 372, 379 729- 730C 358, 365 986 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 277 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91202 M 85 SKIN & PELAGE BLACK SKIN VENTRAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91203 M MASS(ES) AXILLARY REGION-RIGHT WEEK 76 INGUINAL REGION-LEFT WEEK 84 VENTRAL-THORACIC-RIGHT WEEK 84 76 BEHAVIOR RECUMBENT LATERAL DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES 582, 589-590 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183,. 190, 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365,. 372, 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456,. 463, 470 477, 484, 491, 498, 505, 512, 519 526C, 533C', 54 0C, 547C, 554C, 561C , 568C, 575C, 582C, 589-590C 582C, 589-590C 582C, 589-590C 532 526 526 987 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 278 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91203 M 76 EYES SQUINTED PERI-ORBITAL RESPIRATION IRREGULAR SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS C91204 D MASS(ES) AXILLARY REGION-RIGHT WEEK 60 61 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 526, 532 532 532 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 414, 421, 428,. 435, 442, 44 9C,, 456C, 463, 470C, 477C, 484C , 491C 498C, 505C , 512C, 519C, 526C, 532C 358, 365, 372,. 379, 386, 393, 400, 407, 414, 421 , 426 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218, 225, 232 988 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 279 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91204 D 61 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 C91205 1 C91206 M 4 NORMAL NO REMARKABLE OBSERVATIONS 75 APPEARANCE MALOCCLUSION BEHAVIOR HYPOACTIVE EYES PALE EYES RESPIRATION AUDIBLE SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 239C, 246C, 253C, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C 393C, 400C, 407C, 414C, 421C, 426C 1, 8, 15, 22, 24 491, 498, 505, 512 -513, 519 512-513, 519 519 489, 491 513, 519 489, 491, 498,. 505, 512 1, 8,r 15, 22, 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 989 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 280 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91206 M 75 NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE MALOCCLUSION DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYES SKIN & PELAGE PALE ENTIRE BODY YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 731 386 386 617 645 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 990 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 281 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91208 M 75 APPEARANCE HUNCHED THIN EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-LEFT EYES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91209 D 75 NORMAL NO REMARKABLE OBSERVATIONS 518-519 498, 505, 512, 518-519 519 518-519 470, 477 518-519 518-519 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 484 491 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519- 520 991 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 282 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91210 M 75 BEHAVIOR HYPOACTIVE EYES RED DISCHARGE EYES SKIN & PELAGE COLD TO TOUCH ENTIRE BODY RED HAIR COAT HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 51 523 523 523 512, 519, 523 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 372, 379, 386, 393 , 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505 351C, 358C, 365C 992 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 283 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91211 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91212 D MASS(ES) INGUINAL REGION-LEFT WEEK 86 67 APPEARANCE HEAD TILT RIGHT THIN NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 VENTRAL-ABDOMINAL-RIGHT WEEK 49 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589 596C, 60 3C, 610C, 617C, 624C , 631C , 638C, 645C, 652C, 659C, 666C 673C, 68 0C, 687C, 694C, 701C , 708C , 715C, 722C, 729C, 731C 456, 463 , 467 449, 456 , 463, 467 1, 8, 15 , 22, 29, 36, 43, 50 , 57, 64, 71, 78,. 85, 92, 99, 106 113, 120 , 127, 134, 141, 148 , 155, 162, 169, 176, 183,, 190, 197 204, 211 , 218, 225, 232, 239 , 246, 253, 260, 267, 274, 281, 288 295, 302 , 309, 316, 323, 330 337, 344 , 351C, 358C, 365C, 372C, 379C, 386C, 393C, 407C, 414C 421C, 42 8C, 435C, 442C, 449C , 456C , 463C, 467C 400C 993 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 284 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91213 M 77 APPEARANCE HEAD TILT RIGHT THIN EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 535 535 519, 526, 529, 533, 535 529, 533, 535 519, 526, 529, 533, 535 529, 533, 535 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512 994 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 285 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91214 M 99 APPEARANCE MALOCCLUSION THIN EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 75 WEEK 84 INGUINAL REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL-LEFT WEEK 64 WEEK 75 VENTRAL-CERVICAL WEEK 64 589, 596, 603, 610 659, 666, 673, 680, 683, 687 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 , 421 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 , 512 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 , 603 638, 642, 645, 652, 659, 666, 673, 680, 683, 687 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330 519C,. 526C, 533C, 540C, 547C, 554C , 561C 582C,. 589C, 596C, 603C, 610C 582C,. 589C, 596C, 603C, 610C, 617C , 624C, 631C, 638C, 642C , 645C 652C, 659C, 666C, 673C, 680C, 683C , 687C 442C,. 449C, 456C, 463C, 470C, 477C , 484C, 491C, 498C, 505C , 512C 519C,. 526C, 533C, 540C, 547C, 554C , 561C, 568C, 575C, 582C , 589C 596C, 603C, 610C, 617C, 624C, 631C , 638C, 642C, 645C, 652C , 659C 666C, 673C, 680C, 683C, 687C 526C 442C,. 449C, 456C, 463C, 470C, 477C , 484C, 491C, 498C, 505C , 512C 995 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 286 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91214 M 99 MASS(ES) VENTRAL-CERVICAL WEEK 64 WEEK 95 C91215 D 54 APPEARANCE MALOCCLUSION SKIN & PELAGE COLD TO TOUCH LIMBS-HIND NORMAL NO REMARKABLE OBSERVATIONS C91216 T APPEARANCE THIN NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 519C, 526C, 533C, 54 0C, 547C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 642C, 645C, 652C 659C, 666C, 673C, 680C, 683C, 687C 372, 378 372, 378 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645, 652 996 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 287 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91216 T 105 MASS(ES) INGUINAL REGION-RIGHT WEEK 98 C91217 M 68 APPEARANCE THIN EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES RESPIRATION IRREGULAR SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 470, 472 463 470, 472 470, 472 472 463, 470, 472 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456 997 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 288 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91218 M 97 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN BEHAVIOR ATAXIC HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES SKIN & PELAGE SORE/SCAB MOUTH NORMAL NO REMARKABLE OBSERVATIONS 638, 645, 652, 659, 666, 673 617, 624, 631, 638, 645, 652, 659, 666, 673 274 659, 666, 673 673 617, 624 673 589, 617, 673 666 29 1, 8,r 15, 22, 36, 43 , 50,r 57, 64, 71, 78, 85,r 92, 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169 , 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260 , 267, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449 , 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533,. 540 , 547, 554, 561, 568, 575 582, 603, 610 998 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 289 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91219 M 88 APPEARANCE HUNCHED MALOCCLUSION THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 88 610 197, 204, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 600, 603, 609- 610 610 610 600, 603, 609-610 582, 589 596, 600 , 603, 609- 610 1, 8, 15 113, 120 218, 225 309, 316 400 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 211 232, 239 , 246, 253, 260, 267 , 274, 281, 288, 295 , 302 323, 330 , 337, 344, 351, 358 , 365, 372, 379, 386 , 393 610C 999 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 290 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91220 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL WEEK 82 VENTRAL-ABDOMINAL-LEFT WEEK 82 C91221 T VENTRAL-ABDOMINAL-RIGHT WEEK 97 105 APPEARANCE MALOCCLUSION 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127 , 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218 , 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309 , 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400 , 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491 , 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 582C, 589C , 5 96C, 603C, 610C, 617C , 624C , 631C, 638C, 645C , 652C 659C, 666C , 6 73C, 680C, 687C, 694C , 701C , 708C, 715C, 722C , 72 9C 732C 568, 575, 582 , 589, 596C , 603C , 61 0C, 617C, 624C , 631C , 638C 645C, 652C , 659C, 666C, 673C, 680C , 687C , 694C, 701C, 708C , 715C 722C,. 72 9C , 732C 568C, 575C , 582C, 589C, 596C, 603C , 610C , 617C, 624C, 631C , 638C 645C, 652C , 659C, 666C, 673C, 680C , 687C , 694C, 701C, 708C , 715C 722C,. 72 9C , 732C 673, 680, 687 , 694C , 701C, 708C, 715C, 722C, 729C, 732C 148, 155, 162 , 169, 176, 183, 190, 197, 204, 211 , 218, 225 , 232 239, 246, 253 , 260, 267, 274, 281, 288, 295, 302 , 309, 316 , 323 330, 337, 344 , 351, 358, 365, 372, 400, 407, 414 , 421, 428 , 435 442, 449, 456 , 463, 470, 477, 484, 491, 498, 505 , 512, 519 , 526 533, 540, 547 , 554, 561, 568, 575, 582, 589, 596 , 603, 610 , 617 624, 631, 638 , 645, 652, 659, 666, 673, 680, 687 , 694, 701 , 708 715, 722, 729-730 1000 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 291 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91221 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91222 1 C91223 3 C91224 M 4 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 97 APPEARANCE MALOCCLUSION THIN DISCHARGE GENITAL RED IN COLOR NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,. 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99 113, 120, 127,, 134, 141, 379, 386, 393 1, 8,. 15, 22, 24 1, 8,. 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 589, 596, 603, 610 659, 666, 673, 676 676 676 656, 659, 666, 673, 676 656, 676 676 1001 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 292 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91224 M 97 NORMAL NO REMARKABLE OBSERVATIONS C91225 M MASS(ES) INGUINAL REGION-LEFT WEEK 87 INGUINAL REGION-RIGHT WEEK 72 WEEK 87 84 APPEARANCE MALOCCLUSION MISSING TEETH SWOLLEN VENTRAL-ABDOMINAL THIN EXCRETION FEW FECES DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 568 , 575, 582, 652 603, 610, 617, 624 , 631, 638, 645 498C, 505C , 512C, 519C, 526C, 533C , 540C, 547C, 554C, 561C 603, 610, 617, 624 , 631, 638, 645 15, 22, 29', 36,r 43, 50, 57, 64 , 71,r 78, 85, 92, 99, 106 , 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 , 190, 197, 204 211,. 218, 225, 232, 239, 246, 253, 260, 267, 274 , 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 , 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 , 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 , 554, 561, 568 575,. 582, 585 463,. 470, 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547 554, 561, 568, 575, 582, 585 582, 585 568,. 575, 582, 585 568,. 575, 582, 585 1002 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 293 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91225 M 84 EYES PALE EYES SKIN & PELAGE COLD TO TOUCH ENTIRE BODY PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS C91226 M 86 APPEARANCE HEAD TILT LEFT RIGHT THIN BEHAVIOR HYPOACTIVE EYES RED DISCHARGE EYES RESPIRATION AUDIBLE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 585 582, 585 582, 585 1, 8 596 582, 589 582, 589, 596 596 568, 575, 582 596 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 1003 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 294 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91226 M 86 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 59 AXILLARY REGION-RIGHT WEEK 38 INGUINAL REGION-LEFT WEEK 64 C91227 T INGUINAL REGION-RIGHT WEEK 74 VENTRAL-ABDOMINAL WEEK 35 VENTRAL-CERVICAL-RIGHT WEEK 78 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 211, 218, 225, 232 407, 414, 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C 484C, 491C , 498C , 505C , 512C , 519C , 526C , 533C , 540C , 547C, 554C 561C, 568C , 575C , 582C , 589C , 596C 260, 267, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C 337C, 344C , 351C , 358C , 365C , 372C , 379C , 386C , 393C , 400C, 407 442C, 449C , 456C , 463C , 470C , 477C , 484C , 491C , 498C , 505C, 512C 519C, 526C , 533C , 540C , 547C , 554C , 561C , 568C , 575C , 582C, 589C 596C 512C, 519C , 526C , 533C , 540C , 547C , 554C , 561C , 568C , 575C, 582C 58 9C, 596C 239C, 246C , 253C , 260C , 267C , 274C , 281C , 288C , 295C , 302C, 309C 316C, 323C 54 0C, 547C , 554C , 561C , 568C , 575C , 582C , 589C , 596C 589, 596, 603 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 1 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 1004 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 295 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91227 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 86 INGUINAL REGION-RIGHT WEEK 84 C91228 M 94 EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449, 456,, 463, 470 477, 484, 491, 498 , 505,, 512, 519, 526, 533, 540, 547,, 554, 561 568, 575 596C , 603C, 610C, 617C, 624C, 631C, 638C , 645C, 652C, 659C, 666C 673C , 680C, 687C, 694C, 701C, 708C, 715C , 722C, 729-730C 582C , 589C, 596C, 603C, 610C, 617C, 624C , 631C, 638C, 645C, 652C 659C , 666C, 673C, 680C, 687C, 694C, 701C , 708C, 715C, 722C 729- 730C 638, 645, 652 638, 645, 652 624, 631, 638, 645, 652 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 372, 379 , 386, 393, 400, 407 , 414 421, 428, 435,. 442, 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526,. 533, 540, 547, 554, 561 1005 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 296 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91228 M 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 VENTRAL-ABDOMINAL WEEK 49 C91229 M 94 APPEARANCE THIN RESPIRATION IRREGULAR SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 WEEK 82 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C 337C, 344C, 351C, 358C, 365C 645, 652 652 596, 603, 610, 617, 624, 631, 638, 645 , 652 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 505,. 512, 519, 526, 533, 540 , 547, 554, 561 491, 498 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C 1006 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 297 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91230 1 C91231 M 4 NORMAL NO REMARKABLE OBSERVATIONS 92 BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL RESPIRATION LABORED SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 638-639 639 638-639 638-639 639 631, 638-639 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 498 , 505 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610, 617, 624 1007 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 298 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91231 M 92 WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 67 C91232 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91233 3 C91234 D 14 NORMAL NO REMARKABLE OBSERVATIONS 66 NORMAL NO REMARKABLE OBSERVATIONS C91235 T 105 EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-LEFT MOUTH DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 463, 470, 477, 484 , 491 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 722, 729, 731 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 94 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 459 568 568 582 1008 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 299 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91235 T 105 NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 93 INGUINAL REGION-LEFT WEEK 94 VENTRAL-ABDOMINAL WEEK 92 VENTRAL-ABDOMINAL-LEFT WEEK 46 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 575, 589, 596, 603, 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694, 701, 708, 715, 722 , 729, 731 589, 596, 603, 610 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183 , 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 , 274 , 281 , 288 295, 302, 309, 330, 337, 344, 351, 358, 365, 372 , 379 , 386 , 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 , 477 , 484 491, 498, 505,. 512, 519, 526, 533, 540, 547, 554 , 561 , 568 , 575 582, 617, 624, 631 645, 652, 659,. 666, 673, 680, 687, 694C, 701C , 7 08C, 715C, 722C 729- 730C 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 638C 316, 323 1009 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 300 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91237 1 C91238 M 4 NORMAL NO REMARKABLE OBSERVATIONS 68 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS M 58 APPEARANCE MALOCCLUSION EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT 1, 8, 15, 22, 24 456, 463, 470 463, 470 456, 463, 470 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449 225, 232, 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302 , 309 316, 323, 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 134, 141, 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 211 , 218 323, 330, 337, 344 , 351, 358, 365 1010 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98. 1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 301 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91239 M 58 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 47 AXILLARY REGION-RIGHT WEEK 35 C91240 M 79 APPEARANCE MALOCCLUSION RESPIRATION LABORED SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 323, 330C, 337C, 344C, 351C, 358C, 365C, 372C , 379C, 386C, 393C 400C 239C , 246C , 253C, 260C, 267C , 274C , 281C, 288C, 295C, 302C , 309C 316C , 323C , 330C, 337C, 344C , 351C , 358C, 365C, 372C, 379C , 386C 393C , 400C 85, 92, 99 , 106, 113, 120, 127, 134, 141, 148 , 155, 162, 169 176, 183, 190, 197, 204, 211 , 218, 225, 232, 239, 246 , 253 , 260 267, 274, 281, 288, 295, 302 , 309, 316, 323, 330, 337 , 344 , 351 358, 365, 372, 379, 386, 393 , 400, 407, 414, 421, 428 , 435 , 442 449, 456, 463, 470, 477, 484 , 491, 498, 505, 512, 519 , 526 , 533 540, 547, 550 550 435 1, 8, 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78 1011 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 302 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91240 M 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 C91241 3 14 NORMAL NO REMARKABLE OBSERVATIONS C91242 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91243 T 105 SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 68 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 512, 519, 526,, 533, 540, 547C, 550C 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 94 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,, 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582,, 589, 596, 603, 610, 617, 624, 631 , 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 , 729- 730 561, 568, 575,, 582, 589, 596, 603, 610, 617, 624 , 631, 638 , 645 652, 659, 666,, 673, 680, 687, 694, 701, 708, 715 , 722, 729 , 731 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 414, 421, 428,, 435, 442, 449, 456, 463 470C, 477C:, 484C, 491C, 498C, 505C , 512C, 519C, 526C, 533C , 540C 1012 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 303 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91243 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 68 C91244 M INGUINAL REGION-LEFT WEEK 89 INGUINAL REGION-RIGHT WEEK 93 VENTRAL -ABDOMINAL-RIGHT WEEK 56 67 APPEARANCE MALOCCLUSION BEHAVIOR HYPOACTIVE VOCALIZATION DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYES DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 547C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 701C, 708C, 715C, 722C, 729C, 731C 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C 694C, 701C, 708C, 715C, 722C, 729C, 731C 645, 652, 659, 666, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C,. 729C, 731C 386, 393, 400, 407 241, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407, 414 421, 428, 435, 442, 449, 456, 463, 467 467 467 241 241, 246 1013 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 304 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91244 M 67 RESPIRATION AUDIBLE SKIN & PELAGE PALE EARS NORMAL NO REMARKABLE OBSERVATIONS C91245 M 96 APPEARANCE MALOCCLUSION BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 467 241, 246 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239 589, 596, 603 668 659, 666, 668 668 668 659, 666, 668 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1014 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 305 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91245 M 96 NORMAL NO REMARKABLE OBSERVATIONS C91246 M 87 APPEARANCE HUNCHED THIN EXCRETION NO FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 68 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 610, 617, 624, 631, 638, 645, 652 582, 589, 596, 600, 603- 604 603-604 600, 603-604 603-604 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 600C, 603-604C 1015 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 306 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91247 M 73 APPEARANCE THIN BEHAVIOR RECUMBENT STERNAL DISCHARGE ORAL RED IN COLOR EXCRETION NO FECES EYES RED DISCHARGE EYES RESPIRATION AUDIBLE LABORED SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 470, 477, 484, 491, 498, 505, 508 508 508 470, 508 470, 477, 484, 491, 498, 505, 508 508 508 456, 463 470, 477, 484, 491 1, 8, 15 113, 120 204, 211 316, 323 407, 414 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 295, 302 , 309 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 421, 428 , 435 1016 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 307 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91247 M 73 MASS(ES) INGUINAL REGION-LEFT WEEK 64 VENTRAL-ABDOMINAL WEEK 40 VENTRAL-ABDOMINAL-RIGHT WEEK 70 C91248 3 14 NORMAL NO REMARKABLE OBSERVATIONS C91249 M 97 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES PALE EYES RESPIRATION LABORED DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 508C 274C, 281C, 288C 484C, 491C, 498C, 505C, 508C 1, 8, 15, 22 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 484, 491, 498, 505 657, 659, 666, 673 673 673 652, 657, 659, 666, 673 638, 645, 652, 657, 659, 666, 673 673 1017 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 308 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91249 M 97 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE PERINEAL AREA VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91250 M 73 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 638, 645, 652, 657 645, 652, 657, 659, 666 673 673 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 512, 519, 526 , 533, 540, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617 , 624, 631 176, 183, 190, 197 , 204, 211, 218, 225 , 232, 239, 246, 253 , 260 267, 274, 281, 288 , 295, 302, 309, 316 , 323, 330, 337, 344 , 351 358, 365, 372, 379 , 386, 393, 400, 407 , 414, 421, 428, 435 , 442 449, 456, 463, 470 , 477, 484, 491, 498 , 505 498, 505 1, 8, 15 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 127,, 134, 141, 148, 155,. 162, 169 1018 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 309 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F2 (CONTINUED FROM PREVIOUS PAGE) C91250 M 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 DOSE: 3 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 414C, 421C, 42 8C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C 491C, 498C, 505C 1019 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 92 MASS(ES) AXILLARY REGION-LEFT WEEK 92 93 MASS(ES) 98 AXILLARY REGION-LEFT WEEK 92 76 MASS(ES) 77 INGUINAL REGION-RIGHT WEEK 76 83 MASS(ES) 84 INGUINAL REGION-RIGHT WEEK 83 85 MASS(ES) 86 INGUINAL REGION-RIGHT WEEK 83 87 MASS(ES) 94 INGUINAL REGION-RIGHT WEEK 83 95 MASS(ES) 96 INGUINAL REGION-RIGHT WEEK 83 97 MASS(ES) 98 INGUINAL REGION-RIGHT WEEK 83 15 X 15MM 20 X 20MM 15 X 15MM 30 X 20MM 35 X 25MM 50 X 40MM 55 X 70MM 70 X 70MM 1020 Covance 6329-212 3M T-6316.1 PAGE: 310 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 86 MASS(ES) 87 AXILLARY REGION-LEFT WEEK 85 88 MASS(ES) 91 AXILLARY REGION-LEFT WEEK 85 71 MASS(ES) 75 AXILLARY REGION-RIGHT WEEK 71 76 MASS(ES) 81 AXILLARY REGION-RIGHT WEEK 71 82 MASS(ES) AXILLARY REGION-RIGHT WEEK 71 83 MASS(ES) 86 AXILLARY REGION-RIGHT WEEK 71 87 MASS(ES) 94 AXILLARY REGION-RIGHT WEEK 71 95 MASS(ES) 10 AXILLARY REGION-RIGHT WEEK 71 10 X 10MM 25 X 20MM 20 X 20MM 30 X 35MM 60 X 40MM 60 X 60MM 70 X 70MM 65 X 60MM 1021 Covance 6329-212 3M T-6316.1 PAGE: 311 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91195 C91196 F2 603 87 TO 617 89 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 624 90 TO 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 645 93 MASS(ES) TO 694 100 AXILLARY REGION-RIGHT WEEK 87 F2 554 80 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 565 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 568 82 TO 575 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 579 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 579 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 10 X 20MM 20 X 20MM 45 X 35MM 10 X 10MM 25 X 20MM 30 X 30MM 50 X 30MM 50 X 30MM 1022 Covance 6329-212 3M T-6316.1 PAGE: 312 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 83 MASS(ES) INGUINAL REGION-RIGHT WEEK 80 83 MASS(ES) 84 HEAD WEEK 82 85 MASS(ES) HEAD WEEK 82 86 MASS(ES) HEAD WEEK 82 87 MASS(ES) 87 HEAD WEEK 82 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 85 MASS(ES) 89 AXILLARY REGION-RIGHT WEEK 74 90 MASS(ES) 91 AXILLARY REGION-RIGHT WEEK 74 50 X 30MM 10 X 20MM 10 X 25MM 10 X 30MM 30 X 35MM 10 X 20MM 15 X 25MM 20 X 20MM 1023 Covance 6329-212 3M T-6316.1 PAGE: 313 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 92 MASS(ES) 93 AXILLARY REGION-RIGHT WEEK 74 94 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 95 MASS(ES) 101 AXILLARY REGION-RIGHT WEEK 74 102 MASS(ES) 104 AXILLARY REGION-RIGHT WEEK 74 105 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 74 97 MASS(ES) 101 INGUINAL REGION-RIGHT WEEK 97 102 MASS(ES) 105 INGUINAL REGION-RIGHT WEEK 97 49 MASS(ES) 51 VENTRAL-ABDOMINAL-RIGHT WEEK 49 30 X 25MM 35 X 30MM 40 X 30MM 60 X 50MM 60 X 45MM 10 X 10MM 50 X 30MM 25 X 20MM 1024 Covance 6329-212 3M T-6316.1 PAGE: 314 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91201 C91202 C91203 F2 729 105 MASS(ES) TO 730 105 VENTRAL-ABDOMINAL-RIGHT WEEK 105 F2 526 76 TO 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 568 82 TO 590 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 76 582 84 TO 590 85 MASS(ES) INGUINAL REGION-LEFT WEEK 84 582 84 TO 590 85 MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 84 F2 449 65 TO 456 66 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 470 68 TO 512 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 519 75 TO 532 76 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 20 X 20MM 30 X 20MM 40 X40MM 20 X 10MM 10 X 15MM 10 X 10MM 30 X 30MM 40 X 40MM 1025 Covance 6329-212 3M T-6316.1 PAGE: 315 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91204 C91210 F2 239 35 TO 302 44 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 309 45 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 330 48 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 372 54 TO 426 61 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 F2 351 51 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL WEEK 51 F2 596 86 TO 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 86 652 94 MASS(ES) TO 722 104 INGUINAL REGION-LEFT WEEK 86 729 105 MASS(ES) TO 731 105 INGUINAL REGION-LEFT WEEK 86 30 X 25MM 50 X 40MM 45 X 30MM 45 X 35MM 15 X 15MM 30 X 30MM 20 X 20MM 20 X 40MM 1026 Covance 6329-212 3M T-6316.1 PAGE: 316 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 51 MASS(ES) 53 VENTRAL-ABDOMINAL-LEFT WEEK 49 54 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 55 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 56 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 57 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 60 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 10 X 10MM 15 X 15MM 20 X 15MM 20 X 15MM 20 X 15MM 20 X 15MM 10 X 10MM 10 X 10MM 1027 Covance 6329-212 3M T-6316.1 PAGE: 317 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91212 F2 428 62 435 63 442 64 449 65 456 66 463 67 467 67 400 58 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 49 10 X 10M 10 X 10MM 10 X 10MM 10 X 10MM 10 X 10MM 10 X 10MM 10 X 10MM 20 X 15MM 1028 Covance 6329-212 3M T-6316.1 PAGE: 318 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 75 MASS(ES) 81 INGUINAL REGION-LEFT WEEK 75 84 MASS(ES) 88 INGUINAL REGION-LEFT WEEK 84 84 MASS(ES) 99 INGUINAL REGION-RIGHT WEEK 84 64 MASS(ES) 67 VENTRAL-ABDOMINAL-LEFT WEEK 64 68 MASS(ES) 70 VENTRAL-ABDOMINAL-LEFT WEEK 64 71 MASS(ES) 74 VENTRAL-ABDOMINAL-LEFT WEEK 64 75 MASS(ES) 79 VENTRAL-ABDOMINAL-LEFT WEEK 64 80 MASS(ES) 81 VENTRAL-ABDOMINAL-LEFT WEEK 64 20 X 20MM 10 X 10MM 10 X 10MM 15 X 15MM 20 X 25MM 30 X 30MM 35 X 40MM 35 X 55MM 1029 Covance 6329-212 3M T-6316.1 PAGE: 319 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 82 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 64 85 MASS(ES) 86 VENTRAL-ABDOMINAL-LEFT WEEK 64 87 MASS(ES) 91 VENTRAL-ABDOMINAL-LEFT WEEK 64 92 MASS(ES) 94 VENTRAL-ABDOMINAL-LEFT WEEK 64 95 MASS(ES) 96 VENTRAL-ABDOMINAL-LEFT WEEK 64 97 MASS(ES) 99 VENTRAL-ABDOMINAL-LEFT WEEK 64 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 64 MASS(ES) 67 VENTRAL-CERVICAL WEEK 64 40 X 70MM 60 X 80MM 60 X 90MM 70 X 100MM 90 X 50MM 50 X 90MM 35 X 40MM 10 X 10MM 1030 Covance 6329-212 3M T-6316.1 PAGE: 320 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 68 MASS(ES) 74 VENTRAL-CERVICAL WEEK 64 75 MASS(ES) VENTRAL-CERVICAL WEEK 64 76 MASS(ES) 78 VENTRAL-CERVICAL WEEK 64 79 MASS(ES) 81 VENTRAL-CERVICAL WEEK 64 82 MASS(ES) 84 VENTRAL-CERVICAL WEEK 64 85 MASS(ES) VENTRAL-CERVICAL WEEK 64 86 MASS(ES) VENTRAL-CERVICAL WEEK 64 87 MASS(ES) VENTRAL-CERVICAL WEEK 64 25 X 25MM 35 X 35MM 40 X 40MM 50 X 40MM 60 X 50MM 70 X 60MM 80 X 70MM 85 X 75MM 1031 Covance 6329-212 3M T-6316.1 PAGE: 321 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91214 C91216 F2 610 88 TO 631 91 MASS(ES) VENTRAL-CERVICAL WEEK 64 638 92 TO 652 94 MASS(ES) VENTRAL-CERVICAL WEEK 64 659 95 TO 683 98 MASS(ES) VENTRAL-CERVICAL WEEK 95 687 99 TO 687 99 MASS(ES) VENTRAL-CERVICAL WEEK 95 687 99 MASS(ES) VENTRAL-CERVICAL WEEK 95 F2 680 98 MASS(ES) TO 701 101 INGUINAL REGION-RIGHT WEEK 98 708 102 MASS(ES) TO 722 104 INGUINAL REGION-RIGHT WEEK 98 729 105 MASS(ES) TO 730 105 INGUINAL REGION-RIGHT WEEK 98 90 X 80MM 100 X 85MM 75 X 90MM 85 X 90MM 85 X 90MM 10 X 10MM 50 X 40MM 50 X 30MM 1032 Covance 6329-212 3M T-6316.1 PAGE: 322 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91219 C91220 F2 610 88 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL WEEK 88 F2 582 84 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 603 87 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 638 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 645 93 TO 659 95 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 666 96 TO 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 694 100 MASS(ES) TO 732 105 AXILLARY REGION-RIGHT WEEK 84 596 86 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 10 X 10MM 10 X 15MM 15 X 15MM 25 X 25MM 30 X 25MM 30 X 30MM 40 X 35MM 10 X 10MM 1033 Covance 6329-212 3M T-6316.1 PAGE: 323 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91220 F2 603 87 TO 610 88 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 617 89 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 638 92 TO 645 93 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 652 94 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 659 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 666 96 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 82 694 100 MASS(ES) TO 732 105 VENTRAL-ABDOMINAL WEEK 82 568 82 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 15 X 15MM 20 X 20MM 25 X 25MM 10 X 10MM 20 X 25MM 30 X 30MM 20 X 20MM 20 X 20MM 1034 Covance 6329-212 3M T-6316.1 PAGE: 324 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 87 MASS(ES) 91 VENTRAL-ABDOMINAL-LEFT WEEK 82 92 MASS(ES) 93 VENTRAL-ABDOMINAL-LEFT WEEK 82 94 MASS(ES) 95 VENTRAL-ABDOMINAL-LEFT WEEK 82 96 MASS(ES) 99 VENTRAL-ABDOMINAL-LEFT WEEK 82 100 MASS(ES) 101 VENTRAL-ABDOMINAL-LEFT WEEK 82 102 MASS(ES) 105 VENTRAL-ABDOMINAL-LEFT WEEK 82 100 MASS(ES) 101 VENTRAL-ABDOMINAL-RIGHT WEEK 97 102 MASS(ES) 105 VENTRAL-ABDOMINAL-RIGHT WEEK 97 25 X 25MM 30 X 40MM 30 X 30MM 55 X 50MM 65 X 55MM 70 X 70MM 25 X 25MM 40 X 30MM 1035 Covance 6329-212 3M T-6316.1 PAGE: 325 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91224 C91226 F2 498 72 TO 561 81 F2 421 61 TO 435 63 442 64 TO 561 81 568 82 TO 596 86 274 40 TO 400 58 442 64 TO 498 72 505 73 TO 512 74 519 75 TO 561 81 MASS(ES) INGUINAL REGION-RIGHT WEEK 72 MASS(ES) AXILLARY REGION-LEFT WEEK 59 MASS(ES) AXILLARY REGION-LEFT WEEK 59 MASS(ES) AXILLARY REGION-LEFT WEEK 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 MASS(ES) INGUINAL REGION-LEFT WEEK 64 MASS(ES) INGUINAL REGION-LEFT WEEK 64 MASS(ES) INGUINAL REGION-LEFT WEEK 64 10 X 15MM 15 X15MM 35 X 30MM 40 X 45MM 10 X 10MM 15 X 15MM 20 X 20MM 30 X 50MM 1036 Covance 6329-212 3M T-6316.1 PAGE: 326 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 82 MASS(ES) 86 INGUINAL REGION-LEFT WEEK 64 74 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 75 MASS(ES) 82 INGUINAL REGION-RIGHT WEEK 74 83 MASS(ES) 85 INGUINAL REGION-RIGHT WEEK 74 86 MASS(ES) 86 INGUINAL REGION-RIGHT WEEK 74 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 74 35 MASS(ES) 47 VENTRAL-ABDOMINAL WEEK 35 78 MASS(ES) 81 VENTRAL-CERVICAL-RIGHT WEEK 78 40 X 60MM 15 X 15MM 20 X 20MM 30 X 30MM 40 X 40MM 40 X 40MM 20 X 20MM 20 X 20MM 1037 Covance 6329-212 3M T-6316.1 PAGE: 327 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91226 C91227 F2 568 82 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 78 575 83 TO 596 86 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 78 F2 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 86 603 87 TO 631 91 MASS(ES) INGUINAL REGION-LEFT WEEK 86 638 92 TO 645 93 MASS(ES) INGUINAL REGION-LEFT WEEK 86 652 94 TO 680 98 MASS(ES) INGUINAL REGION-LEFT WEEK 86 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 86 694 100 MASS(ES) TO 701 101 INGUINAL REGION-LEFT WEEK 86 20 X 30MM 30 X 30MM 25 X 25MM 25 X 35MM 50 X 40MM 60 X 50MM 60 X 60MM 70 X 65MM 1038 Covance 6329-212 3M T-6316.1 PAGE: 328 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91227 F2 708 102 MASS(ES) TO 730 105 INGUINAL REGION-LEFT WEEK 86 582 84 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 589 85 TO 596 86 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 603 87 TO 617 89 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 624 90 TO 631 91 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 638 92 TO 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 694 100 MASS(ES) TO 701 101 INGUINAL REGION-RIGHT WEEK 84 85 X 65MM 15 X 10 MM 25 X 25MM 35 X 35MM 40 X 40MM 50 X 45MM 70 X 60MM 85 X 60 1039 Covance 6329-212 3M T-6316.1 PAGE: 329 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 10. MASS(ES) 10 INGUINAL REGION-RIGHT WEEK 84 82 MASS(ES) 85 AXILLARY REGION-RIGHT WEEK 82 86 MASS(ES) 89 AXILLARY REGION-RIGHT WEEK 82 90 MASS(ES) 94 AXILLARY REGION-RIGHT WEEK 82 49 MASS(ES) 53 VENTRAL-ABDOMINAL WEEK 49 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 100 X 70MM 20 X 20MM 30 X 25MM 25 X 20MM 10 X 10MM 10 X10MM 20 X 20MM 25 X 30MM 1040 Covance 6329-212 3M T-6316.1 PAGE: 330 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91229 C91236 F2 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 603 87 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 638 92 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 82 F2 694 100 MASS(ES) TO 730 105 AXILLARY REGION-RIGHT WEEK 93 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 94 659 95 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 94 694 100 MASS(ES) TO 722 104 INGUINAL REGION-LEFT WEEK 94 638 92 MASS(ES) VENTRAL-ABDOMINAL WEEK 92 30 X 35MM 35 X 35MM 50 X 45MM 15 X 15MM 10 X 10MM 20 X 20MM 25 X 25MM 15 X 15MM 1041 Covance 6329-212 3M T-6316.1 PAGE: 331 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 48 MASS(ES) 58 AXILLARY REGION-LEFT WEEK 47 35 MASS(ES) 38 AXILLARY REGION-RIGHT WEEK 35 39 MASS(ES) 46 AXILLARY REGION-RIGHT WEEK 35 47 MASS(ES) 49 AXILLARY REGION-RIGHT WEEK 35 50 MASS(ES) 51 AXILLARY REGION-RIGHT WEEK 35 52 MASS(ES) 53 AXILLARY REGION-RIGHT WEEK 35 54 MASS(ES) 58 AXILLARY REGION-RIGHT WEEK 35 79 MASS(ES) 79 AXILLARY REGION-RIGHT WEEK 74 10 X 10MM 10 X 10MM 15 X 20MM 20 X 20MM 20 X 25MM 30 X 30MM 45 X 45MM 10 X 10MM 1042 Covance 6329-212 3M T-6316.1 PAGE: 332 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 68 MASS(ES) 70 AXILLARY REGION-LEFT WEEK 68 71 MASS(ES) 81 AXILLARY REGION-LEFT WEEK 68 82 MASS(ES) 84 AXILLARY REGION-LEFT WEEK 68 85 MASS(ES) 86 AXILLARY REGION-LEFT WEEK 68 87 MASS(ES) AXILLARY REGION-LEFT WEEK 68 88 MASS(ES) 92 AXILLARY REGION-LEFT WEEK 68 93 MASS(ES) AXILLARY REGION-LEFT WEEK 68 94 MASS(ES) AXILLARY REGION-LEFT WEEK 68 10 X 10MM 25 X 25MM 40 X 40MM 45 X 45MM 60 X 45MM 65 X 55MM 70 X 60MM 85 X 60MM 1043 Covance 6329-212 3M T-6316.1 PAGE: 333 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91243 C91246 F2 659 95 MASS(ES) AXILLARY REGION-LEFT WEEK 68 666 96 MASS(ES) TO 731 105 AXILLARY REGION-LEFT WEEK 68 617 89 TO 652 94 MASS(ES) INGUINAL REGION-LEFT WEEK 89 659 95 MASS(ES) TO 731 105 INGUINAL REGION-LEFT WEEK 89 673 97 TO 680 98 MASS(ES) INGUINAL REGION-RIGHT WEEK 93 687 99 MASS(ES) TO 731 105 INGUINAL REGION-RIGHT WEEK 93 F2 470 68 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 68 484 70 TO 491 71 MASS(ES) AXILLARY REGION-LEFT WEEK 68 75 X 50MM 75 X 60MM 20 X 15MM 30 X 40MM 15 X 15MM 25 X 25MM 15 X 15MM 20 X 20MM 1044 Covance 6329-212 3M T-6316.1 PAGE: 334 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F2 498 72 TO 512 74 519 75 TO 540 78 547 79 TO 561 81 568 82 575 83 TO 582 84 589 85 596 86 TO 600 86 603 87 TO 604 87 MASS(ES) AXILLARY REGION-LEFT WEEK 68 MASS(ES) AXILLARY REGION-LEFT WEEK 68 MASS(ES) AXILLARY REGION-LEFT WEEK 68 MASS(ES) AXILLARY REGION-LEFT WEEK 68 MASS(ES) AXILLARY REGION-LEFT WEEK 68 MASS(ES) AXILLARY REGION-LEFT WEEK 68 MASS(ES) AXILLARY REGION-LEFT WEEK 68 MASS(ES) AXILLARY REGION-LEFT WEEK 68 30 X 20MM 40 X 30MM 50 X 30MM 60 X 45MM 65 X 60MM 75 X 70MM 100 X 70MM 105 X 75MM 1045 Covance 6329-212 3M T-6316.1 PAGE: 335 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 64 MASS(ES) 69 INGUINAL REGION-LEFT WEEK 64 70 MASS(ES) 73 INGUINAL REGION-LEFT WEEK 64 73 MASS(ES) 73 INGUINAL REGION-LEFT WEEK 64 40 MASS(ES) 42 VENTRAL-ABDOMINAL WEEK 40 70 MASS(ES) 73 VENTRAL-ABDOMINAL-RIGHT WEEK 70 38 MASS(ES) 39 AXILLARY REGION-RIGHT WEEK 38 40 MASS(ES) 45 AXILLARY REGION-RIGHT WEEK 38 46 MASS(ES) 47 AXILLARY REGION-RIGHT WEEK 38 15 X 15MM 25 X 25MM 30 X 30MM 10 X 20MM 20 X 20MM 10 X 10MM 20 X 20MM 35 X 35MM 1046 Covance 6329-212 3M T-6316.1 PAGE: 336 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 48 MASS(ES) 50 AXILLARY REGION-RIGHT WEEK 38 51 MASS(ES) 54 AXILLARY REGION-RIGHT WEEK 38 55 MASS(ES) 58 AXILLARY REGION-RIGHT WEEK 38 59 MASS(ES) 60 AXILLARY REGION-RIGHT WEEK 38 61 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 62 MASS(ES) 63 AXILLARY REGION-RIGHT WEEK 38 64 MASS(ES) 72 AXILLARY REGION-RIGHT WEEK 38 73 MASS(ES) 73 AXILLARY REGION-RIGHT WEEK 38 40 X 35MM 50 X 45MM 60 X 55MM 70 X 60MM 80 X 65MM 80 X 80MM 90 X 85MM 100 X 100MM 1047 Covance 6329-212 3M T-6316.1 PAGE: 337 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91250 F2 505 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 38 90 X 85MM Covance 6329-212 3M T-6316.1 PAGE: 338 1048 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 339 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91251 1 C91252 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C91253 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 105 1, 8, 15, 22, 24 589, 596, 603,. 610, 617, 624, 631, 638 , 645 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582,. 652, 659, 666, 673, 680 , 687, 694, 701, 708 , 715 722, 729- 730 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722 729-730C 1049 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 340 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91254 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C91255 M 74 APPEARANCE HUNCHED BEHAVIOR HYPOACTIVE RECUMBENT STERNAL EYES SQUINTED PERI-ORBITAL RESPIRATION LABORED SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT 512, 519, 526,. 533, 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610, 617,. 624, 631, 638, 645, 652 , 659, 666, 673, 680 , 687 694, 701, 708,. 715, 722, 729, 731 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505 512-513 512-513 513 512-513 513 498, 505, 512-513 1050 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 341 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91255 M 74 NORMAL NO REMARKABLE OBSERVATIONS ASS(ES) AXILLARY REGION-LEFT WEEK 64 VENTRAL-ABDOMINAL-RIGHT WEEK 62 C91256 T 105 APPEARANCE SWOLLEN PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 90 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155,. 162, 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337,. 344, 351, 358, 365, 372, 379 386, 393, 400, 407 , 414, 421 442, 449, 456, 463 , 470C, 477C , 484C, 491C, 498C, 505C , 512-513C 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512-513C 652, 659, 666,. 673, 680, 687, 694, 701 , 708, 715, 722, 729 , 731 1, 8,, 15, 22, 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 701C, 708C', 715C, 722C, 72 9C, 731C 1051 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 342 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91257 M 83 APPEARANCE HUNCHED MALOCCLUSION THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 68 579 554, 561, 568-570, 575, 579 540, 547,. 554, 561, 568-570, 575 568- 570, 575, 579 570, 575 64, 71, 120, 127, 134, 162, 169, 176, 183, 190, 197, 204, 211 218, 225 232, 239 , 246, 253, 260, 267 , 274, 281, 288, 295, 302 309, 316 323, 330 , 337, 344, 351, 358 , 365, 372, 379, 386, 393 400, 407 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477, 484 491, 498 505, 512 , 519, 526, 533,. 540 , 547, 554, 561, 568- 570 575, 579 579 1, 8, 15 22, 29, 36, 43,r 50, 57, 78, 85, 92, 99, 106, 113, 141 148, 155 470, 477 1052 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 343 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91258 M 86 APPEARANCE HUNCHED THIN EXCRETION FEW FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 75 VENTRAL-ABDOMINAL-LEFT WEEK 71 VENTRAL-THORACIC-LEFT WEEK 79 600 600 600 600 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484 519C, 526C 533C 491C, 498C 505C, 512C, 519C, 526C, 533C, 54 0C, 547C, 554C, 561C 568C, 575C 582C, 58 9C, 596C, 600C 547C, 554C 561C, 568C, 575C, 582C, 58 9C, 596C, 600C 1053 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 344 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91259 D 63 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR ATAXIC HYPOACTIVE EYES RED DISCHARGE EYE-RIGHT EYES NORMAL NO REMARKABLE OBSERVATIONS C91260 M 73 APPEARANCE THIN BEHAVIOR HYPOACTIVE RECUMBENT LATERAL EXCRETION FEW FECES 435, 438 421, 428, 435, 438 435, 438 428, 435, 438 435, 438 393, 400 407, 414, 421, 428 1, 8, 15 113, 120 204, 211 295, 302 386 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 498, 505, 509 509 509 491, 498, 505, 509 1054 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 345 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91260 M 73 EYES RED DISCHARGE EYES RESPIRATION LABORED SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS D 99 APPEARANCE MALOCCLUSION THIN SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 491, 498, 505, 509 509 498, 505, 509 491, 498, 505, 509 1, 8,r 15, 22, 29, 36 , 43, 50 113, 120, 127, 134, 141, 148 204, 211, 218, 225, 232, 239 295, 302, 309, 316, 323, 330 386, 393, 400, 407, 414, 421 477, 484 57, 64, 71, 78, 85 155, 162 , 169, 176 246, 253 , 260, 267 337, 344 , 351, 358 428, 435 , 442, 449 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 463, 470, 477, 484, 491 673, 680, 687, 689 568, 575, 582, 589, 596, 603 610, 617, 624, 631 638, 645, 652 659, 666, 673, 680, 687, 689 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 1055 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 346 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91261 D 99 NORMAL NO REMARKABLE OBSERVATIONS C91262 M MASS(ES) AXILLARY REGION-RIGHT WEEK 87 89 APPEARANCE DEHYDRATED HUNCHED THIN EXCRETION NO FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 498, 505 512, 519, 526, 533, 540, 547, 554, 561 603C, 610C, 617C, 624C 631C 638C, 645C, 652C, 659C 666C, 673C 680C, 687C, 689C 617-618 617-618 610, 617-618 617-618 610, 617-618 617-618 1, 8, 15 113, 120 204, 211 295, 302 386, 463 547, 554 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 470, 477 , 484, 491, 498, 505 , 512, 519, 526, 533 , 540 1056 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 347 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91262 M 89 MASS(ES) VENTRAL-ABDOMINAL WEEK 57 VENTRAL-THORACIC WEEK 81 C91263 M 72 APPEARANCE THIN BEHAVIOR ATAXIC HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 561, 568, 575, 582, 589, 596, 603, 610, 617-618 484, 491, 498 498 498 484, 498 456, 463, 484, 498 498 463 456, 463 1, 8,, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 113, 120, 127,, 134, 141 1057 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 348 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91263 M 72 MASS(ES) AXILLARY REGION-LEFT WEEK 33 AXILLARY REGION-RIGHT WEEK 31 VENTRAL-ABDOMINAL-LEFT WEEK 22 C91264 T 105 SKIN & PELAGE ALOPECIA NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 97 AXILLARY REGION-RIGHT WEEK 85 WEEK 93 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 22 5C, 232C , 239C, 246C, 253C 211, 218, 225 148, 155, 162, 169, 176C , 183C, 190C, 197C, 204C, 211C , 218C 22 5C, 232C , 239C, 246C, 253C , 260C , 267C, 274C, 281C, 288C , 295C 302C,. 309C , 316C, 323C, 330C , 337C , 344C, 351C, 358C, 365C , 372C 379C, 386C , 393C, 400C, 407C , 414C , 421C, 428C, 435C, 442C , 449C 456C, 463C , 470C, 477C, 484C , 491C , 498C 568, 575, 617, 624, 631 1, 8,. 15, 22, 29, 36, 43 , 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 , 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239 , 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330 , 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421 , 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512 , 519, 526, 533, 540, 547, 554 , 561 582, 638 673, 680, 687C, 694C, 70 1C, '708C, 715C, 722C, 729-730C 589, 596, 603, 610 645C, 652C , 659C, 666C, 673C , 680C , 687C, 694C, 701C, 708C , 715C 722C, 729- 730C 1058 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 349 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91265 M 94 APPEARANCE HUNCHED MALOCCLUSION THIN BEHAVIOR ATAXIC HYPOACTIVE EXCRETION FEW FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91266 M 104 APPEARANCE HEAD TILT RIGHT THIN EXCRETION FEW FECES NO FECES 653 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652- 653 653 653 653 645, 652-653 653 1, 8,, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 113, 120 723 713, 715, 722-723 713 715, 722-723 1059 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 350 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91266 M 104 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL BLACK SKIN VENTRAL-ABDOMINAL-LEFT SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 97 INGUINAL REGION-RIGHT WEEK 89 VENTRAL-ABDOMINAL-RIGHT WEEK 40 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 713, 715, 722723 713, 715, 722- 723 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 , 652, 659 , 666 673, 680, 687, 694, 701, 708, 713, 715, 722- 723 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85 , 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 , 358, 365 , 372 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 , 449, 456 , 463 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 , 540, 547 , 554 561, 568, 575 673, 680, 687, 694C, 701C, 708C, 713C, 715C, 722 -723C 617C, 624C , 631C, 638C, 645C, 652C , 659C, 666C, 673C, 680C , 687C 694C, 701C , 708C, 713C, 715C, 722- 723C 274C, 281C , 288C, 295C, 302C, 309C , 316C, 323C, 330C, 337, 344 351 1060 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 351 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91267 M 67 SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-RIGHT SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 VENTRAL-CERVICAL-LEFT WEEK 46 C91268 M 97 EYES DISCOLORED/DARK RED EYE-LEFT SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 358, 365 372, 379, 386, 393 , 400, 407, 414, 421, 428, 435, 442, 449 , 456 463- 464 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 260C , 267C, 274C, 281C, 288C, 295C , 302C , 309C, 316C, 323C , 330C 337C , 344C, 351C, 358C, 365C, 372C , 379C , 386C, 393C, 400C , 407C 414C , 421C, 428C, 435C, 442C, 449C , 456C , 463-464C 316C , 323C, 330C, 337C, 344C, 351C , 358C , 365C, 372C, 379C , 386C 393C , 400C, 407C, 414C, 421C, 428C , 435C , 442C, 449C, 456C 463- 464C 645, 652, 659, 666, 673 673 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1061 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 352 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91268 M 97 NORMAL NO REMARKABLE OBSERVATIONS C91269 M MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 83 APPEARANCE DEHYDRATED HEAD TILT LEFT THIN BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-LEFT DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141,. 148, 155, 162, 169, 176 , 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 , 456, 463, 470 477, 484, 491, 498, 505,. 512, 519, 526, 533, 540 , 547 554C, 561C , 568C, 575C, 582C, 58 9C, 596C , 603C, 610C, 617C, 624C 631C, 638C , 645C, 652C, 659C, 666C, 673C 568-569 578 566, 568-569, 575, 578 566, 568-569, 575, 578 557, 566, 568-569, 575, 578 551, 554, 575 557, 566, 568-569, 578 578 1062 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 353 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91269 M 83 EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91270 M 59 APPEARANCE HEAD TILT LEFT THIN BEHAVIOR CIRCLING LEFT EXCRETION NO FECES EYES RED DISCHARGE EYES DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 566, 568-569, 575 547, 551, 554, 557 551, 554, 557, 561, 566, 568-569, 575, 578 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 407 386, 393, 400, 407 407 386 386 1063 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 354 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91270 M 59 RESPIRATION LABORED SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C91271 M 94 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE DISCHARGE GENITAL RED IN COLOR EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE YELLOW HAIR COAT DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 407 386 386, 393, 400, 407 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 645, 652-653 653 653 653 505, 645, 652-653 653 1064 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 355 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91271 M 94 NORMAL NO REMARKABLE OBSERVATIONS C91272 1 C91273 M MASS(ES) AXILLARY REGION-LEFT WEEK 84 AXILLARY REGION-RIGHT WEEK 89 INGUINAL REGION-LEFT WEEK 75 WEEK 84 4 NORMAL NO REMARKABLE OBSERVATIONS 81 APPEARANCE HEAD TILT RIGHT EYES RED DISCHARGE EYE-LEFT DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 512 582, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652- 653C 617, 624, 631, 638, 645, 652-653 519, 526, 533, 540, 547, 554C, 561C , 568C, 575C 582C , 589C , 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652- 653C 1, 8, 15, 22, 24 561 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540 1065 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 356 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91273 M 81 NORMAL NO REMARKABLE OBSERVATIONS C91274 D MASS(ES) INGUINAL REGION-LEFT WEEK 76 70 NORMAL NO REMARKABLE OBSERVATIONS C91275 M 69 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 526, 533, 540, 547, 554, 561 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 490 463, 470, 477, 480 442, 449 470, 477, 480 435, 442, 449, 456, 463, 470, 477, 480 1066 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 357 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91275 M 69 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91276 M MASS(ES) AXILLARY REGION-LEFT WEEK 58 AXILLARY REGION-RIGHT WEEK 52 97 APPEARANCE MALOCCLUSION THIN EYES PALE EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 435, 442, 449, 456 435, 442, 449, 456, 463, 470 , 477, 480 1, 8,r 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 , 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239 , 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 , 337, 344 , 351 400, 407, 414C , 421C, 428C, 435C, 442C , 449C, 456C , 463C, 470C 477C, 480C 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 568, 575, 673 526 673 421, 428 673 1067 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 358 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91276 M 97 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT LATERAL-ABDOMINAL-RIGHT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 82 AXILLARY REGION-RIGHT WEEK 93 DORSAL-CERVICAL-LEFT WEEK 82 INGUINAL REGION-LEFT WEEK 88 VENTRAL-ABDOMINAL WEEK 72 VENTRAL-ABDOMINAL-RIGHT WEEK 62 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 617, 624, 631, 638 673 526, 533, 540, 547, 554, 561, 568, 575 , 582, 589, 596, 603 , 610 617, 624, 631,. 638, 645, 652, 659, 666 , 673 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414 568C, 575C , 582C, 58 9C, 596C, 603C, 610C , 617C , 624C , 631C , 638C 645C, 652C , 659C, 666C, 673C 645, 652, 659, 666 , 673 568C, 575C , 582C, 58 9C, 596C, 603C, 610C , 617C , 624C , 631C , 638C 645C, 652C , 659C, 666C, 673C 610C, 617C , 624C, 631C, 638C, 645C, 652C , 659C , 666C , 673C 498C, 505C , 512C, 519C, 526C, 533C, 540C , 547C , 554C , 561C , 568C 575C, 582C , 589C, 596C, 603C, 610C, 617C , 624C , 631C , 638C , 645C 652C, 659C , 666C, 673C 428, 435, 442, 449 , 456,r 463, 470, 477, 484C, 491C, 498C, 505C 512C, 519C , 526C, 533C, 54 0C, 547C, 554C , 561C , 568C , 575C , 582C 1068 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 359 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91276 M 97 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 C91277 M 72 APPEARANCE HUNCHED MALOCCLUSION THIN EYES RED DISCHARGE EYES SKIN & PELAGE COLD TO TOUCH ENTIRE BODY PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 WEEK 72 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C, 673C 498 141, 148, 155, 162, 169, 176, 183, 190 498 498 498 498 1, 8,r 15, 22, 29, 36, 43,r 50,. 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127,. 134, 197, 204, 211, 218, 225, 232, 239 , 246, 253 260, 267, 274,. 281, 288, 295, 302, 309, 316, 323, 330 , 337, 344 351, 358, 365,. 372, 379, 386, 393, 400, 407, 414, 421 428, 435C,, 442C, 449C, 456C, 463C, 470C, 477C , 484C, 491C, 498C 498C 1069 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 360 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91278 T 105 EYES CLEAR DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS C91279 T MASS(ES) AXILLARY REGION-LEFT WEEK 105 TAIL-PROXIMAL WEEK 86 VENTRAL-CERVICAL-RIGHT WEEK 105 105 DISCHARGE ORAL RED IN COLOR SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT 568 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 575, 582, 589,. 610, 617, 624, 631, 638 , 645, 652, 659, 666 , 673 680, 687, 694, 701, 708, 715, 722 729-730C 596, 603 729-730C 493 568, 575, 582, 589 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729, 731 617, 624, 631 1070 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 361 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91279 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 96 INGUINAL REGION-LEFT WEEK 80 INGUINAL REGION-RIGHT WEEK 86 VENTRAL-ABDOMINAL-LEFT WEEK 53 C91280 M 98 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC HYPOACTIVE EXCRETION FEW FECES DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141 , 148, 155, 162, 169, 176, 183 , 190 , 197 204, 211, 218, 225 , 232 , 239, 246, 253, 260, 267, 274 , 281 , 288 295, 302, 309, 316 , 323 , 330, 337, 344, 351, 358 666, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729C, 731C 554C, 561C, 568C, 575C, 582C, 589C , 596C , 603C, 610C, 617C , 624C 631C, 638C, 645C, 652C, 659C, 666C , 673C , 680C, 687C, 694C , 701C 708C, 715C, 722C, 729C, 731C 596, 603, 610 365C, 372C, 379C, 386C, 393C, 400C , 407C , 414C, 421C, 428C , 435C 442C, 449C, 456C, 463C, 470C, 477C , 484C , 491C, 493C, 498C , 505C 512C, 519C, 526C, 533C, 54 0C, 547C , 554C , 561C, 568C, 575C , 582C 58 9C, 596C, 603C, 610C, 617C, 624C , 631C , 638C, 645C, 652C , 659C 666C, 673C, 680C, 687C, 694C, 701C , 708C , 715C, 722C, 72 9C , 731C 671, 673, 680-681 681 681 671, 673, 680-681 671, 673, 680-681 1071 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 362 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91280 M 98 EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS 3 14 NORMAL NO REMARKABLE OBSERVATIONS M 53 BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 671, 673, 680-681 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 94 358, 365 351, 356, 358, 365 358, 365 356, 358, 365 358, 365 1072 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 363 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91282 M 53 RESPIRATION AUDIBLE LABORED SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS C91283 M WART LIKE LESION(S) VENTRAL-THORACIC WEEK 46 93 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC HYPOACTIVE EXCRETION FEW FECES NO FECES DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 365 365 351, 356, 358, 365 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309 316, 323, 330, 337, 344, 351 629 617, 624, 629, 631, 638, 645, 647 647 629 610, 647 617, 629 1073 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 364 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91283 M 93 EYES RED DISCHARGE EYES SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB DORSAL PAW-HIND-RIGHT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 82 VENTRAL-ABDOMINAL-RIGHT WEEK 82 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 629 647 498, 505 617, 624, 629, 631, 638, 645, 647 617, 624, 629, 631, 638, 645, 647 1, 8,r 15, 22, 29, 36 , 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155 , 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246 , 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337 , 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428 , 435 , 442, 449, 456, 463, 470 477, 484, 491,. 512, 519, 526, 533 , 540 , 547, 554, 561 568, 575, 582,. 589C, 596C , 603C, 610C, 617C, 624C,, 629C, 631C 638C, 645C, 647C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 638C, 645C, 647C 1074 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 365 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91284 D 100 NORMAL NO REMARKABLE OBSERVATIONS C91285 1 C91286 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 NORMAL NO REMARKABLE OBSERVATIONS C91287 1 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8,, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694 1, 8,, 15, 22, 24 1, 8,, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 1, 8,, 15, 22, 24 1075 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 366 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91288 3 C91289 M 14 NORMAL NO REMARKABLE OBSERVATIONS 73 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN BEHAVIOR ATAXIC DISCHARGE ORAL CLEAR EXCRETION FEW FECES RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE COLD TO TOUCH LIMBS-ALL 1, 8,r 15, 22, 29, , 43,r 50, 57, 64, 71, 78, 85, 92, 94 484, 491, 498, 505 508 141, 148, 155, 162 232, 239, 246, 253 323, 330, 337, 344 414, 421, 428, 435 505, 508 491, 498, 505, 508 508 169, 176, 183, 190 , 197, 204, 211, 218, 225 260, 267, 274, 281 , 288, 295, 302, 309, 316 351, 358, 365, 372 , 379, 386, 393, 400, 407 442, 449, 456, 463 , 470, 477, 484, 491, 498 508 508 484, 491, 498, 505 508 508 508 498 508 1076 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 367 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91289 M 73 SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS M 59 APPEARANCE HEAD TILT RIGHT MALOCCLUSION THIN SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 52 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 421, 428 1, 8, 15 , 22, 29, 36 , 43 , 50 , 57, 64, 71 , 78, 85, 92, 99, 106 113, 120 , 127, 134 393, 400 , 407 43, 50, 57, 64 , 71, 78, 85, 92, 99 , 106, 113,, 120, 127 , 134, 141 148, 155 , 162, 169, 176, 183 , 190, 197, 204, 211, 218, 225, 232 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302, 309, 316, 323 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393, 400, 407 393, 400 , 407 372 1, 8, 15, 22, 29, 36 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C 1077 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 368 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91291 1 C91292 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C91293 3 C91294 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION EYES CLEAR DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 24 351, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582, 589 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729-730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 358, 365, 372, 379 , 386 393, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 1, 8,, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 603, 610, 617,, 624, 631 498, 505 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 1078 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 369 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91294 T 105 NORMAL NO REMARKABLE OBSERVATIONS T 105 NORMAL NO REMARKABLE OBSERVATIONS C91296 M MASS(ES) AXILLARY REGION-LEFT WEEK 81 WEEK 87 VENTRAL -ABDOMINAL-LEFT WEEK 99 86 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491,. 512, 519, 526, 533, 540 , 547, 554, 561, 568 , 575 582, 589, 596,. 638, 645, 652, 659, 666 , 673, 680, 687, 694 , 701 708, 715, 722,. 729, 731 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 582 5c8o9o, 5C9O6 561, 568, 575 603, 610, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C, 694C , 701C, 708C, 715C , 722C, 729C, 732C 687C,. 694C, 701C , 708C, 715C, 722C , 729C, 732C 594, 596 582, 587, 589, 594, 596 596 582, 587, 589, 594 1079 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 370 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91296 M 86 EYES RED DISCHARGE EYES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91297 D 91 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 90 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 582, 587, 589, 594, 596 594, 596 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 624C, 631C, 636C 1080 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 371 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91298 M 74 APPEARANCE MALOCCLUSION THIN SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 43 3 14 NORMAL NO REMARKABLE OBSERVATIONS 148, 155, 162, 169, 176, 183 , 190, 197, 204, 211 , 218, 225 , 232 239, 246, 253, 260, 267, 274 , 281, 288, 295, 302 , 309, 316 , 323 330, 337, 344, 351, 358, 365 , 372, 379, 386, 393 , 400, 407 , 414 421, 428, 435, 442, 449, 456 , 463, 470, 477, 484 , 491, 498 , 505 512 477, 484, 491, 498, 505, 512 400, 407 477, 484, 491, 498, 505, 512 1, 8,, 15, 22, 29, 36, 43 , 50 , 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127, 134, 141 295, 302, 309C , 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C 372C, 379C , 386C, 393C, 400C , 407C , 414C, 421C, 428C, 435C , 442C 449C, 456C , 463C, 470C, 477C , 484C , 491C, 498C, 505C, 512C 1, 8,, 15, 22, 29, 36, 43 , 50 , 57, 64, 71, 78, 85 , 92, 94 1081 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 372 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91300 D 83 APPEARANCE THIN BEHAVIOR CIRCLING LEFT EXCRETION FEW FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL RESPIRATION AUDIBLE SKIN & PELAGE PALE ENTIRE BODY YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 498, 505, 512, 519, 526, 533, 536, 540, 547, 549, 554, 561, 564 568, 570, 575, 579 549, 554 570, 575, 579 536, 540, 547, 549, 554, 561, 564, 568, 570, 575, 579 526, 533 575, 579 570 536, 540, 547,. 549, 554, 561, 564, 568 , 570, 575, 579 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 435, 442 , 449 456, 463, 470, 477, 484, 491 1082 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 373 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91300 D 83 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 C91301 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91302 M MASS(ES) AXILLARY REGION-RIGHT WEEK 99 INGUINAL REGION-LEFT WEEK 105 72 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION DISCOLORED URINE RED IN COLOR FEW FECES DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127 , 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218 , 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309 , 316 , 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400 , 407 , 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491 , 498 , 505, 512, 519, 526, 533, 540, 547, 554 , 561 568, 575, 582 , 589 , 596, 603, 610, 617, 624, 631, 638, 645 , 652 659, 666, 673 , 680 687C, 694C , 7 01C, 708C, 715C, 722C , 729- 730C 729-730C 484, 491, 498, 501, 504 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 501, 504 428, 435, 442, 449, 504 407 504 1083 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 374 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91302 M 72 EYES RED DISCHARGE EYES RESPIRATION AUDIBLE SKIN & PELAGE COLD TO TOUCH ENTIRE BODY ROUGH HAIR COAT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91303 T MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 105 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 414, 421, 428, 435, 442, 449 501 504 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 501, 504 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 501, 504 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 1 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 2 295, 302, 309, 316 , 323, 330, 337, 344 , 400 351C, 358C, 365C, 372C, 379C, 386C, 393C 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 1084 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 375 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 (CONTINUED FROM PREVIOUS PAGE) C91303 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 105 VENTRAL-ABDOMINAL-LEFT WEEK 100 C91304 3 14 SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91305 M 87 APPEARANCE CONVULSIONS PARALYSIS LIMBS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 659, 666, 673, 680, 687 729-730 694C, 701C, 708C, 715C, 722C, 729- 730C 15, 22, 29, 36,, 43, 50, 57, 64, 71 , 78, 85, 92, 94 15, 22, 29, 36,, 43, 50, 57, 64, 71 , 78, 85, 92, 94 1, 8 288, 295, 302, 309, 316,, 372, 379, 386, 400, 407 603-604 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 323 330, 337, 344 351, 358, 365, 393, 414 , 421, 428, 435, 442 , 449 456, 463, 470 477, 484, 491, 498, 505 , 512, 519, 526, 533 , 540 547, 554, 561 568, 575, 582, 589, 596 1085 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 376 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91306 M 103 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 74 AXILLARY REGION-RIGHT WEEK 85 INGUINAL REGION-LEFT WEEK 81 WEEK 93 INGUINAL REGION-RIGHT WEEK 92 372, 379, 386, 393 , 400, 407, 414, 421, 428, 435 , 442, 449, 456 463, 470, 477, 484 , 491 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 10 6 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176 , 183,, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 498, 505 512, 519, 526, 533 , 540, 547, 554, 561, 568, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C , 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C , 715C 589C, 596C, 603C, 610C, 617C, 624C , 631C, 638C, 645C, 652C, 659C 666C, 673C, 680C, 687C, 694C, 701C , 708C, 715C 561C, 568C, 575C, 582C, 58 9C, 596C , 603C, 610C, 617C, 624C, 631C 645C, 652C, 659C, 666C, 673C, 680C , 687C, 694C, 701C, 708C, 715C 638C, 645C, 652C, 659C, 666C, 673C , 680C, 687C, 694C, 701C, 708C 715C 1086 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 377 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91307 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91308 M 69 APPEARANCE HEAD TILT RIGHT BEHAVIOR ATAXIC SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 470, 477, 480 480 15, 22 1, 8, 29 127, 134 218, 225 309, 316 400, 407 36, 43, 50, 57,, 64, 71, 78, 85, 92, 99, 106, 113, 120 141,. 148, 155, 162, 169, 176 , 183, 190, 197, 204, 211 232, 239, 246, 253, 260, 267 , 274, 281, 288, 295, 302 323, 330, 337, 344, 351, 358 , 365, 372, 379, 386, 393 414, 421, 428, 435, 442, 449 , 456, 463 1087 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 378 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F3 DOSE: 30 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91309 M 67 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS C91310 T MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 VENTRAL-CERVICAL WEEK 62 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 71 428, 435, 442, 449 , 456, 463- 464 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C 463-464C 42 8C, 435C, 442C, 449C, 456C, 463-464C 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 547C, 554C, 561C 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C 722C, 729C, 732C 1088 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91253 C91255 F3 729 105 MASS(ES) TO 730 105 VENTRAL-ABDOMINAL-LEFT WEEK 105 F3 470 68 TO 477 69 MASS(ES) AXILLARY REGION-LEFT WEEK 64 484 70 MASS(ES) AXILLARY REGION-LEFT WEEK 64 491 71 TO 498 72 MASS(ES) AXILLARY REGION-LEFT WEEK 64 505 73 TO 513 74 MASS(ES) AXILLARY REGION-LEFT WEEK 64 428 62 TO 463 67 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 470 68 TO 484 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 491 71 TO 513 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 30 X 30MM 10 X 10MM 20 X 20MM 30 X 30MM 10 X 10MM 10 X 10MM 15 X 20MM 20 X 30MM 1089 Covance 6329-212 3M T-6316.1 PAGE: 379 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91256 C91258 F3 645 93 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 666 96 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 687 99 MASS(ES) VENTRAL-ABDOMINAL WEEK 90 694 100 MASS(ES) TO 731 105 VENTRAL-ABDOMINAL WEEK 90 F3 519 75 TO 533 77 MASS(ES) AXILLARY REGION-LEFT WEEK 75 491 71 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 10 X 10MM 20 X 20MM 30 X 30MM 40 X 30MM 15 X 15MM 15 X 15MM 20 X 20MM 20 X 20MM 1090 Covance 6329-212 3M T-6316.1 PAGE: 380 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91258 F3 533 77 540 78 547 79 554 80 561 81 568 82 575 83 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 20 X 20MM 20 X 20MM 20 X 20MM 20 X 20MM 20 X 20MM 30 X 30MM 30 X 30MM 30 X 45MM 1091 Covance 6329-212 3M T-6316.1 PAGE: 381 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 79 MASS(ES) 81 VENTRAL-THORACIC-LEFT WEEK 79 82 MASS(ES) 84 VENTRAL-THORACIC-LEFT WEEK 79 85 MASS(ES) 86 VENTRAL-THORACIC-LEFT WEEK 79 45 X 50MM 45 X 50MM 45 X 50MM 45 X 50MM 45 X 50MM 20 X 20MM 30 X 30MM 40 X 30MM 1092 Covance 6329-212 3M T-6316.1 PAGE: 382 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 87 88 MASS(ES) 92 AXILLARY REGION-RIGHT WEEK 87 93 MASS(ES) 95 AXILLARY REGION-RIGHT WEEK 87 96 MASS(ES) 99 AXILLARY REGION-RIGHT WEEK 87 33 MASS(ES) 37 AXILLARY REGION-LEFT WEEK 33 26 MASS(ES) 34 VENTRAL-ABDOMINAL-LEFT WEEK 22 35 MASS(ES) 44 VENTRAL-ABDOMINAL-LEFT WEEK 22 45 MASS(ES) 48 VENTRAL-ABDOMINAL-LEFT WEEK 22 30 X 30MM 40 X 35MM 50 X 45MM 60 X 70MM 10 X 10MM 10 X 10MM 30 X 30MM 20 X 20MM 1093 Covance 6329-212 3M T-6316.1 PAGE: 383 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91263 C91264 F3 337 49 TO 386 56 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 22 393 57 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 22 428 62 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 22 F3 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 97 694 100 MASS(ES) TO 730 105 AXILLARY REGION-LEFT WEEK 97 645 93 TO 680 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 687 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 93 694 100 MASS(ES) TO 730 105 AXILLARY REGION-RIGHT WEEK 93 30 X 30MM 40 X 35MM 40 X 40MM 15 X 15MM 25 X 25MM 10 X 10MM 15 X 15MM 20 X 20MM 1094 Covance 6329-212 3M T-6316.1 PAGE: 384 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 100 MASS(ES) 104 AXILLARY REGION-RIGHT WEEK 97 89 MASS(ES) 102 INGUINAL REGION-RIGHT WEEK 89 102 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 103 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 104 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 104 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 104 MASS(ES) INGUINAL REGION-RIGHT WEEK 89 40 MASS(ES) 48 VENTRAL-ABDOMINAL-RIGHT WEEK 40 10 X 10MM 20 X 20MM 20 X 20MM 20 X 20MM 20 X 20MM 20 X 20MM 20 X 20MM 10 X 10MM 1095 Covance 6329-212 3M T-6316.1 PAGE: 385 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 38 MASS(ES) 41 VENTRAL-ABDOMINAL-RIGHT WEEK 38 42 MASS(ES) 50 VENTRAL-ABDOMINAL-RIGHT WEEK 38 51 MASS(ES) 53 VENTRAL-ABDOMINAL-RIGHT WEEK 38 54 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 38 55 MASS(ES) 57 VENTRAL-ABDOMINAL-RIGHT WEEK 38 58 MASS(ES) 63 VENTRAL-ABDOMINAL-RIGHT WEEK 38 64 MASS(ES) 67 VENTRAL-ABDOMINAL-RIGHT WEEK 38 46 MASS(ES) 47 VENTRAL-CERVICAL-LEFT WEEK 46 10 X 10MM 20 X 25MM 25 X 30MM 40 X 40MM 20 X 20MM 20 X 35MM 25 X 35MM 10 X 10MM 1096 Covance 6329-212 3M T-6316.1 PAGE: 386 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 48 MASS(ES) 50 VENTRAL-CERVICAL-LEFT WEEK 46 51 MASS(ES) 55 VENTRAL-CERVICAL-LEFT WEEK 46 56 MASS(ES) 59 VENTRAL-CERVICAL-LEFT WEEK 46 60 MASS(ES) 61 VENTRAL-CERVICAL-LEFT WEEK 46 62 MASS(ES) 63 VENTRAL-CERVICAL-LEFT WEEK 46 64 MASS(ES) 67 VENTRAL-CERVICAL-LEFT WEEK 46 80 MASS(ES) 83 HEAD-MAXILLARY-LEFT WEEK 80 84 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 20 X 20MM 30 X 25MM 35 X 30MM 45 X 55MM 60 X 55MM 70 X 70MM 10 X 10MM 15 X 20MM 1097 Covance 6329-212 3M T-6316.1 PAGE: 387 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 86 MASS(ES) 97 HEAD-MAXILLARY-LEFT WEEK 80 97 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 80 85 MASS(ES) AXILLARY REGION-LEFT WEEK 84 86 MASS(ES) AXILLARY REGION-LEFT WEEK 84 87 MASS(ES) 91 AXILLARY REGION-LEFT WEEK 84 92 MASS(ES) AXILLARY REGION-LEFT WEEK 84 93 MASS(ES) 94 AXILLARY REGION-LEFT WEEK 84 25 X 25MM 30 X 30MM 30 X 40MM 15 X 15MM 20 X 15 30 X 30MM 30 X 40MM 40 X 40MM 1098 Covance 6329-212 3M T-6316.1 PAGE: 388 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 94 MASS(ES) 94 AXILLARY REGION-LEFT WEEK 84 94 MASS(ES) AXILLARY REGION-LEFT WEEK 84 80 MASS(ES) 83 INGUINAL REGION-LEFT WEEK 75 84 MASS(ES) INGUINAL REGION-LEFT WEEK 84 85 MASS(ES) 86 INGUINAL REGION-LEFT WEEK 84 87 MASS(ES) 94 INGUINAL REGION-LEFT WEEK 84 60 MASS(ES) 61 AXILLARY REGION-LEFT WEEK 58 62 MASS(ES) AXILLARY REGION-LEFT WEEK 58 30 X 30MM 30 X 30MM 20 X 20MM 20 X 30 NN 30 X 45MM 35 X 50MM 20 X 15MM 20 X 20MM 1099 Covance 6329-212 3M T-6316.1 PAGE: 389 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91275 C91276 F3 435 63 TO 480 69 358 52 TO 407 59 F3 568 82 TO 582 84 589 85 TO 603 87 610 88 617 89 TO 673 97 568 82 TO 575 83 582 84 MASS(ES) AXILLARY REGION-LEFT WEEK 58 MASS(ES) AXILLARY REGION-RIGHT WEEK 52 MASS(ES) AXILLARY REGION-LEFT WEEK 82 MASS(ES) AXILLARY REGION-LEFT WEEK 82 MASS(ES) AXILLARY REGION-LEFT WEEK 82 MASS(ES) AXILLARY REGION-LEFT WEEK 82 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 25 X 25MM 10 X 10MM 10 X10MM 15 X 15MM 20 X 20MM 30 X 20MM 20 X2 0MM 20 X 30MM 1100 Covance 6329-212 3M T-6316.1 PAGE: 390 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 86 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 87 MASS(ES) DORSAL-CERVICAL-LEFT WEEK 82 88 MASS(ES) 92 DORSAL-CERVICAL-LEFT WEEK 82 93 MASS(ES) 97 DORSAL-CERVICAL-LEFT WEEK 82 88 MASS(ES) 95 INGUINAL REGION-LEFT WEEK 88 96 MASS(ES) 97 INGUINAL REGION-LEFT WEEK 88 72 MASS(ES) 84 VENTRAL-ABDOMINAL WEEK 72 35 X 30MM 40 X 35MM 45 X 35MM 55 X 45MM 70 X 60MM 20 X 20MM 30 X 20MM 20 X 20MM 1101 Covance 6329-212 3M T-6316.1 PAGE: 391 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) 86 VENTRAL-ABDOMINAL WEEK 72 87 MASS(ES) 97 VENTRAL-ABDOMINAL WEEK 72 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 62 71 MASS(ES) 84 VENTRAL-ABDOMINAL-RIGHT WEEK 62 85 MASS(ES) 92 VENTRAL-ABDOMINAL-RIGHT WEEK 62 93 MASS(ES) 97 VENTRAL-ABDOMINAL-RIGHT WEEK 62 63 MASS(ES) 66 VENTRAL-ABDOMINAL-LEFT WEEK 62 67 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 62 35 X 35MM 40 X 45MM 10 X 10MM 20 X 20MM 20 X 35MM 30 X 40MM 20 X 20MM 25MM X 25MM 1102 Covance 6329-212 3M T-6316.1 PAGE: 392 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 68 MASS(ES) 69 VENTRAL-ABDOMINAL-LEFT WEEK 62 70 MASS(ES) 72 VENTRAL-ABDOMINAL-LEFT WEEK 62 72 MASS(ES) 72 VENTRAL-ABDOMINAL-LEFT WEEK 72 105 MASS(ES) 105 AXILLARY REGION-LEFT WEEK 105 105 MASS(ES) 105 VENTRAL-CERVICAL-RIGHT WEEK 105 97 MASS(ES) 99 AXILLARY REGION-RIGHT WEEK 96 100 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 96 80 MASS(ES) 92 INGUINAL REGION-LEFT WEEK 80 55 X 30MM 80 X 50MM 30 X 50MM 10 X 10MM 20 X 20MM 20 X 20MM 35 X 30MM 20 X 20MM 1103 Covance 6329-212 3M T-6316.1 PAGE: 393 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91279 F3 645 93 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 80 694 100 MASS(ES) TO 731 105 INGUINAL REGION-LEFT WEEK 80 365 53 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 372 54 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 414 60 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 484 70 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 519 75 TO 561 81 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 568 82 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 10 X 10MM 20 X 20MM 20 X 30MM 30 X 20MM 15 X 15MM 30 X 20MM 35 X 30MM 40 X 40MM 1104 Covance 6329-212 3M T-6316.1 PAGE: 394 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91279 C91283 F3 589 85 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 53 694 100 MASS(ES) TO 708 102 VENTRAL-ABDOMINAL-LEFT WEEK 53 715 103 MASS(ES) TO 731 105 VENTRAL-ABDOMINAL-LEFT WEEK 53 F3 589 85 TO 596 86 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 603 87 TO 647 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 82 568 82 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 589 85 TO 596 86 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 603 87 TO 647 93 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 82 50 X 50MM 40 X 70MM 70 X 70MM 15 X 15MM 20 X 20MM 20 X 20MM 30 X 30MM 35 X 30MM 1105 Covance 6329-212 3M T-6316.1 PAGE: 395 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91290 F3 358 52 TO 407 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 52 C91295 F3 617 89 TO 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 87 694 100 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 87 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 99 694 100 MASS(ES) TO 732 105 VENTRAL-ABDOMINAL-LEFT WEEK 99 C91297 F3 624 90 TO 636 91 MASS(ES) DORSAL WEEK 90 C91298 F3 309 45 TO 330 48 MASS(ES) AXILLARY REGION-LEFT WEEK 43 337 49 TO 344 50 MASS(ES) AXILLARY REGION-LEFT WEEK 43 10 X 10MM 20 X 25MM 30 X 30MM 40 X 40MM 50 X 55MM 50 X 40 15 X 15MM 20 X 20MM 1106 Covance 6329-212 3M T-6316.1 PAGE: 396 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 51 MASS(ES) 54 AXILLARY REGION-LEFT WEEK 43 55 MASS(ES) 57 AXILLARY REGION-LEFT WEEK 43 58 MASS(ES) 59 AXILLARY REGION-LEFT WEEK 43 60 MASS(ES) AXILLARY REGION-LEFT WEEK 43 61 MASS(ES) 74 AXILLARY REGION-LEFT WEEK 43 53 MASS(ES) 55 VENTRAL-ABDOMINAL-RIGHT WEEK 53 56 MASS(ES) 62 VENTRAL-ABDOMINAL-RIGHT WEEK 53 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 99 30 X 25MM 35 X 25MM 40 X 35MM 55 X 45MM 55 X 55MM 10 X 10MM 20 X 15MM 15 X 15MM 1107 Covance 6329-212 3M T-6316.1 PAGE: 397 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 100 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 99 105 MASS(ES) 105 INGUINAL REGION-LEFT WEEK 105 51 MASS(ES) 57 VENTRAL-ABDOMINAL-RIGHT WEEK 51 100 MASS(ES) 105 VENTRAL-ABDOMINAL-LEFT WEEK 100 83 MASS(ES) 85 AXILLARY REGION-LEFT WEEK 74 86 MASS(ES) 95 AXILLARY REGION-LEFT WEEK 74 96 MASS(ES) AXILLARY REGION-LEFT WEEK 74 97 MASS(ES) 99 AXILLARY REGION-LEFT WEEK 74 30 X 30MM 20 X 20MM 10 X 10MM 10 X 10MM 20 X 20MM 30 X 25MM 30 X 50MM 40 X 50MM 1108 Covance 6329-212 3M T-6316.1 PAGE: 398 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91306 F3 694 100 MASS(ES) TO 715 103 AXILLARY REGION-LEFT WEEK 74 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 596 86 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 610 88 TO 680 98 MASS(ES) AXILLARY REGION-RIGHT WEEK 85 687 99 MASS(ES) TO 715 103 AXILLARY REGION-RIGHT WEEK 85 561 81 TO 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 81 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 81 582 84 TO 589 85 MASS(ES) INGUINAL REGION-LEFT WEEK 81 60 X 50MM 15 X 10 MM 20 X 15MM 30 X 20MM 10 X 10MM 20 X 20MM 40 X 40MM 20X 20MM 1109 Covance 6329-212 3M T-6316.1 PAGE: 399 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91306 C91309 F3 596 86 TO 610 88 MASS(ES) INGUINAL REGION-LEFT WEEK 81 617 89 TO 631 91 MASS(ES) INGUINAL REGION-LEFT WEEK 81 645 93 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 93 694 100 MASS(ES) TO 715 103 INGUINAL REGION-LEFT WEEK 93 638 92 TO 687 99 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 694 100 MASS(ES) TO 715 103 INGUINAL REGION-RIGHT WEEK 92 F3 393 57 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 400 58 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 25 X 20MM 25 X 25MM 10 X 10MM 20 X 20MM 30 X 20MM 35 X 25MM 15 X 15MM 25 X 25MM 1110 Covance 6329-212 3M T-6316.1 PAGE: 400 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 60 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 57 62 MASS(ES) 67 VENTRAL-ABDOMINAL-LEFT WEEK 57 62 MASS(ES) VENTRAL-CERVICAL WEEK 62 63 MASS(ES) 67 VENTRAL-CERVICAL WEEK 62 71 MASS(ES) 72 AXILLARY REGION-LEFT WEEK 71 73 MASS(ES) 81 AXILLARY REGION-LEFT WEEK 71 82 MASS(ES) 84 AXILLARY REGION-LEFT WEEK 71 40 X 40MM 45 X 50MM 40 X 45MM 25 X 25MM 30 X 30MM 10 X 10MM 20 X 20MM 40 X 30MM 1111 Covance 6329-212 3M T-6316.1 PAGE: 401 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91310 F3 589 85 TO 596 86 MASS(ES) AXILLARY REGION-LEFT WEEK 71 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 71 610 88 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 71 638 92 MASS(ES) AXILLARY REGION-LEFT WEEK 71 645 93 TO 680 98 MASS(ES) AXILLARY REGION-LEFT WEEK 71 687 99 MASS(ES) AXILLARY REGION-LEFT WEEK 71 694 100 MASS(ES) TO 722 104 AXILLARY REGION-LEFT WEEK 71 729 105 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 71 50 X 50MM 75 X 50MM 80 X 75MM 80 X 65MM 80 X 75MM 80 X 80MM 80 X 110MM 80 X 95MM 1112 Covance 6329-212 3M T-6316.1 PAGE: 402 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 403 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91311 D 64 NORMAL NO REMARKABLE OBSERVATIONS C91312 M 97 APPEARANCE THIN DISCHARGE GENITAL RED IN COLOR EYES PALE EYES NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 444 526, 533, 540, 547, 554, 561, 568, 575 , 582, 589, 596, 603 , 610 652, 659, 666,. 673, 675 675 526, 533 540, 547, 554, 561, 568 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 617 , 624, 631, 638, 645 1113 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 404 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91313 M 87 APPEARANCE HEAD TILT RIGHT BEHAVIOR HYPOACTIVE SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL VENTRAL-THORACIC NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 74 AXILLARY REGION-RIGHT WEEK 85 VENTRAL-ABDOMINAL WEEK 85 VENTRAL-ABDOMINAL-LEFT WEEK 71 608 608 582, 589, 596, 603 , 607608 582, 589, 596, 603 , 607- 608 1, 8,r 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484 512, 519, 526, 533 , 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 607- 608 58 9C, 596C, 603C, 607-608C 589C, 596C, 603C, 607-608C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 603C, 607-608C 1114 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 405 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91314 M 74 APPEARANCE MALOCCLUSION THIN SKIN & PELAGE ALOPECIA RED HAIR COAT VENTRAL-ABDOMINAL ROUGH HAIR COAT SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 48 VENTRAL-CERVICAL-RIGHT WEEK 61 498, 505, 512 512 99, 302, 309 512 358, 365, 372 120, 134 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 106, 113 127, 141, 148, 155 , 162, 169, 176,. 183, 190, 197, 204, 211, 218 225, 232, 239, 246 , 253, 260, 267,. 274, 281, 288, 295,. 316, 323 379, 386, 393, 400 , 407, 414 330C, 337C, 344C, 351C, 358C, 365C, 372C 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C 1115 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 406 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91315 T 105 EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91316 1 C91317 1 MASS(ES) DORSAL WEEK 99 WEEK 103 4 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 400 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 407,. 414, 421, 428, 435, 442, 449, 456, 463, 470 , 477 484, 491, 498,. 505, 512, 519, 526, 533, 540, 547, 554, 561 , 568 575, 582, 589,. 596, 603, 610, 617, 624, 631, 638, 645, 652 , 659 666, 673, 680 687C, 694C , 701C, 708C, 715C, 722C , 72 9C , 731C 715C, 722C, 729C, 731C 1, 8, 15, 22, 24 1, 8, 15, 22, 24 1116 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 407 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91318 A C91319 M 15 NORMAL NO REMARKABLE OBSERVATIONS 70 BEHAVIOR RECUMBENT LATERAL DISCHARGE ORAL CLEAR EYES RED DISCHARGE EYES RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 102 486 486 486 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484 1117 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 408 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91320 M 79 APPEARANCE THIN BEHAVIOR ATAXIC SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91321 M 94 EYES PALE EYES SKIN & PELAGE SORE/SCAB LUMBAR-LEFT NORMAL NO REMARKABLE OBSERVATIONS 540, 547 547 547 547 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498 , 505, 512, 519, 526 , 533 652, 654 652, 654 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 1118 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 409 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91321 M 94 NORMAL NO REMARKABLE OBSERVATIONS C91322 M MASS(ES) DORSAL WEEK 90 22 APPEARANCE HUNCHED MALOCCLUSION THIN DISCHARGE NASAL RED IN COLOR EXCRETION NO FECES RESPIRATION AUDIBLE SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617 624C, 631C, 638C, 645C, 652C, 654C 150 148, 150-151 148, 150-151 148, 150-151 150-151 150-151 148, 150-151 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141 1119 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 410 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91323 4 53 SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91324 T MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 105 APPEARANCE MALOCCLUSION EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 288, 295, 302,. 309, 316, 323, 330, 337 , 344, 351, 358, 365 , 367 1, 8,, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 351C, 358C , 365C, 367C 400, 407, 414,. 421, 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505, 512 533 582, 589, 596,. 603, 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694, 701, 708, 715, 722 729, 731 1, 8,, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 1120 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 411 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91324 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91325 M 103 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 519, 526, 540, 547, 554, 561, 568, 575 712, 715 701, 708, 712, 715 701, 708, 712, 715 715 712, 715 712, 715 652, 659, 666,. 673, 680, 687, 694, 701 , 708, 712, 715 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 1121 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 412 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91326 M 86 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT LIMBS-FRONT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 582, 589, 596 540, 547, 554, 561, 568, 575, 579, 582 , 589, 596 596 561, 568, 575, 579, 582, 589, 596 579, 582 575, 579, 582, 589, 596 575, 579, 582, 589, 596 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533 1122 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 413 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91327 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 C91328 M 63 APPEARANCE MALOCCLUSION DISCHARGE ORAL RED IN COLOR EYES PALE EYES RED DISCHARGE EYES 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575 582C, 589C , 596C, 603C, 610C, 617C , 624C, 631C, 638C, 645C , 652C 659C, 666C , 673C, 680C, 687C, 694C , 701C, 708C, 715C, 722C , 72 9C 731C 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 437 437 437 437 1123 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 414 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91328 M 63 RESPIRATION AUDIBLE LABORED SKIN & PELAGE RED HAIR COAT DORSAL-CERVICAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91329 3 14 NORMAL NO REMARKABLE OBSERVATIONS C91330 M 85 SKIN & PELAGE SORE/SCAB SACRAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 68 DORSAL-CERVICAL-LEFT WEEK 80 VENTRAL-ABDOMINAL-LEFT WEEK 84 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 437 437 437 1, 8,, 15, 22, 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 1, 8,, 15, 22, 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 94 582, 589- 590 1, 8,, 15, 22, 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C 547C, 554C, 561C, 568C, 575C, 582C, 589-590C 554C, 561C, 568C, 575C, 582C, 589-590C 582, 589-590C 1124 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 415 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91330 M 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 85 VENTRAL-ABDOMINAL-RIGHT WEEK 85 VENTRAL-THORACIC WEEK 54 C91331 M 78 APPEARANCE THIN EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 589-590C 589-590C 372, 379, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589-590C 526, 533, 540-541 523, 526, 533, 540-541 541 523, 526, 533, 540 541 523, 526, 533, 540-541 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 1125 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 416 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91331 M 78 NORMAL NO REMARKABLE OBSERVATIONS C91332 T 105 APPEARANCE THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB DORSAL LATERAL-CERVICAL-LEFT LATERAL-CERVICAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519 582, 589, 596, 603, 610, 701, 708, 715, 722 701, 708, 715, 722 582, 589 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680 687, 694, 701, 708, 715, 722, 729- 730 8, 15, 22, 29 8, 15 1, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 , 204 , 211 , 218 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 , 295 , 302 , 309 316 , 323, 330, 337, 344 , 351, 358, 365, 540 , 547 , 554 , 561 , 568 575 1126 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 417 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91333 1 C91334 D 4 NORMAL NO REMARKABLE OBSERVATIONS 101 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES SKIN & PELAGE SCALY SKIN TAIL-DISTAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 86 VENTRAL-ABDOMINAL WEEK 33 1, 8, 15, 22, 24 407, 414, 421, 428 , 435 , 442 , 449, 456 , 463 , 470 , 477 , 484 , 491 498, 505, 512, 519 , 526 , 533 , 540, 547 , 554 , 561 , 568 , 575 , 582 589, 596, 603, 610 , 617 , 624 , 631, 638 , 645 , 652 , 659 , 666 , 673 676, 680, 687, 691 , 694 , 701 , 703, 705 673, 676, 680, 687 , 691 , 694 , 701, 703 , 705 701, 703, 705 676, 691, 703, 705 36, 43, 50 , 57 , 64 , 71, 78, 85, 92, 99 1, 8, 15, 22, 29, 106, 113, 120, 127, 134, 141, 148, 155, 162 169, 176, 183, 190 , 197 , 204 , 211, 218 , 232 , 239 , 246 , 253 , 260 267, 274, 281, 288 , 295 , 302 , 309, 316 , 323 , 330 , 337 , 344 , 351 358, 365, 372, 379 , 386 , 393 , 400 596, 603, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 676C, 680C, 687C, 691C, 694C, 701C, 703C, 705C 225C 1127 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 418 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91334 D 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 C91335 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91336 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 88 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 673C, 676C, 680C, 687C, 691C, 694C, 701C, 703C, 705C 1, 8 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78 , 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729- 730 1, 8 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78 , 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638C , 645C , 652C, 659C, 666C, 673C , 680C 687C 694C , 701C, 708C, 715C, 722C , 729- 730C 1128 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 419 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91337 3 C91338 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS C91339 M 98 APPEARANCE THIN DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 94 652, 659, 666,, 673, 680, 687, 694, 701 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 708 715, 722, 729, 731 657, 659, 666, 673, 676, 680 657, 659, 666, 673, 676, 680 652, 680 657, 659 652 657, 659, 666, 673, 676, 680 1129 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 420 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91339 M 98 SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91340 T 105 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 652, 657, 659,. 666, 673, 676, 680 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652, 659, 666, 673, 680, 687, 691, 694, 701, 708, 715, 722 729, 731 652, 659, 666, 673, 680, 687, 691, 694, 701, 708, 715, 722, 729 731 701, 708, 715,. 722, 729, 731 691, 694, 701,. 708, 715, 722, 729, 731 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92 1130 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 421 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91340 T 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 VENTRAL-THORACIC-LEFT WEEK 67 C91341 T 105 APPEARANCE CONVULSIONS SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 73 WEEK 75 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673C, 680C, 687C, 691C, 694C, 701C, 708C, 715C 722C, 72 9C, 731C 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C 54 0C, 547C, 554C, 561C, 568C 498, 554, 561, 568, 575, 589 , 596, 603, 659, 666, 673, 687 , 694 701 652, 659, 666, 673, 680, 687 , 694, 701, 708, 715, 722,. 729 -730 1, 8, 15, 22, 29, 36, 43 , 50 , 57, 64, 71, 78,, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 , 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239 , 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330 , 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421 , 428, 435, 442, 449, 456, 463 , 470 477, 484, 491 505, 512, 519C , 526C, 533C, 54 0C, 547C, 554C, 561C, 568C, 575C 582C , 589C , 596C, 603C, 610C , 617C , 624C, 631C, 638C, 645C , 652C 659C , 666C , 673C, 680C, 687C , 694C , 701C, 708C, 715C, 722C 729- 730C 519C , 526C , 533C, 540C, 547C , 554C , 561C, 568C, 575C, 582C , 589C 1131 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 422 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91341 T 105 MASS(ES) VENTRAL-CERVICAL WEEK 75 C91342 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91343 4 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C91344 M 103 APPEARANCE HEAD TILT LEFT HUNCHED MALOCCLUSION THIN DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 596C, 603C, 610C 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 731 302 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 367 713, 715 715 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 713, 715 713, 715 1132 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 423 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91344 M 103 BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS C91345 1 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 4 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 715 713, 715 713, 715 713, 715 1, 8, 15 113, 120 204, 211 295, 302 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 309,. 316, 323, 330, 337, 344, 351, 358 652C, 65 , 666C, 673C, 680C, 687C , 694C, 701C, 708C, 713C , 715C 1, 8, 15, 22, 24 1133 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 424 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91346 T 72 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES CLEAR DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 500-502 500-502 491, 498, 500-502 491, 498, 500-502 500-502 435, 442, 449, 456 , 463, 470, 477, 484 , 491, 498, 500- 502 484, 491, 498, 500 -502 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428 1134 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 425 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91347 4 53 NORMAL NO REMARKABLE OBSERVATIONS C91348 4 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C91349 D 59 APPEARANCE MALOCCLUSION THIN DISCHARGE ORAL RED IN COLOR NASAL RED IN COLOR EYES RED DISCHARGE EYES RESPIRATION AUDIBLE 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 367 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 410 372 268, 271 268, 271 268, 271, 274, 281, 288, 295 268, 271, 274 1135 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 426 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91349 D 59 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT PAWS-HIND YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91350 1 C91351 M MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 17 WART LIKE LESION(S) MOUTH WEEK 23 4 NORMAL NO REMARKABLE OBSERVATIONS 93 BEHAVIOR HYPOACTIVE RESPIRATION LABORED SKIN & PELAGE PALE ENTIRE BODY DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 358, 365, 372 253, 260, 267-268, 271, 274, 281, 288, 295, 302, 309, 316, 323 344, 351, 358, 365, 372, 379,. 386, 393 , 400, 407, 410 1, 8,r 15, 22, 29, 36, 43, 50,. 57, 64, 71, 78 , 85,. 92, 99, 106 120, 127, 134, 141, 148, 176,. 183, 190 , 197, 204, 211,. 218, 225 232, 239, 246 113 155, 162, 169 1, 8, 15, 22, 24 1136 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 427 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91351 M 93 SKIN & PELAGE SORE/SCAB LATERAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91352 4 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 53 NORMAL NO REMARKABLE OBSERVATIONS C91353 4 53 APPEARANCE SWOLLEN PAW-HIND-LEFT SKIN & PELAGE ALOPECIA SORE/SCAB MOUTH VENTRAL-CERVICAL DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 645 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575 582C, 589C , 596C, 603C, 610C, 617C , 624C , 631C, 638C, 645C 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 367 225, 232, 239,. 246, 253 29, 36, 43, 99 225, 232 141, 148 1137 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 428 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91353 4 53 NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) MOUTH WEEK 31 M 88 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES RESPIRATION AUDIBLE SKIN & PELAGE RED HAIR COAT LIMBS-FRONT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 50, 57, 64,r 71, 78, 85, 92, 106 , 113 , 120, 127 134, 155, 162, 169 , 176, 183, 190, 197 , 204, 260, 267, 274, 281 288, 295, 302, 309 , 316, 323, 330, 337 , 344, 351, 358, 365, 367 211, 218 610 463, 470, 477, 484 , 491, 498, 505, 512 , 519, 526, 533, 540, 547 554, 561, 568, 575 , 582, 589, 596, 603 , 610 596, 603, 610 526, 533, 540, 547 , 596 526 596, 603, 610 610 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 1138 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 429 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91354 M 88 NORMAL NO REMARKABLE OBSERVATIONS C91355 3 C91356 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION THIN DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES RESPIRATION AUDIBLE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365,. 372, 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456 1, 8 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 94 206, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 729, 732 206 211 206 1, 8, 15 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 127,, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204 1139 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 430 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91357 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91358 M 75 BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE COLD TO TOUCH ENTIRE BODY YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 523 523 523 523 519,523 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414 1140 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 431 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91358 M 75 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 61 C91359 D 78 APPEARANCE MALOCCLUSION BEHAVIOR SENSITIVE TO TOUCH DISCHARGE GENITAL RED IN COLOR SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 523C 211, 218 260 239, 253, 260 1, 8, 15 113, 120 204, 225 330, 337 421, 428 512, 519 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 232, 246 , 267, 274, 281, 288 , 295, 302, 309, 316 , 323 344, 351 , 358, 365, 372, 379 , 386, 393, 400, 407 , 414 435, 442 , 449, 456, 463, 470 , 477, 484, 491, 498 , 505 526, 533 , 540, 546 1141 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 432 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91360 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C91361 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 372, 379, 386, 393, 400, 407, 414, 421 , 428, 435, 442, 449 , 456 463, 470, 477,. 484, 491, 498, 505, 512 , 519, 526, 533, 540 , 547 554, 561, 568,. 575, 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 659,. 666, 673, 680, 687, 694 , 701, 708, 715, 722 , 729 731 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365 729, 731 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673 680, 687, 694, 701, 708 , 715, 722 1142 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 433 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91362 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C91363 M MASS(ES) AXILLARY REGION-LEFT WEEK 85 AXILLARY REGION-RIGHT WEEK 89 97 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE 596, 603, 610, 617, 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701,. 708, 715, 722, 729, 732 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 58 9C, 596C , 603C, 610C, 617C, 624C, 631C , 638C, 645C, 652C, 659C 666C, 673C , 680C, 687C, 694C, 701C, 708C , 715C, 722C, 729C, 732C 617C, 624C , 631C, 638C, 645C, 652C, 659C , 666C, 673C, 680C, 687C 694C, 701C , 708C, 715C, 722C, 72 9C, 732C 561, 568, 575, 582 , 589,, 596, 603, 610, 617, 624 , 631,, 638, 645 652, 659, 663, 666, 673 673 663, 666, 673 1143 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 434 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91363 M 97 DISCHARGE NASAL RED IN COLOR RESPIRATION AUDIBLE SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 48 MOUTH WEEK 70 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 663 673 663, 666, 673 663, 666, 673 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 358, 365, 372 , 379, 386, 393, 400 , 407 414, 421, 428, 435 , 442, 449, 456, 463 , 470, 477, 491, 498 , 505 512, 519, 526, 533 , 540, 547, 554 330, 337, 344, 351 484 1144 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 435 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91364 D 101 APPEARANCE THIN DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91365 4 53 NORMAL NO REMARKABLE OBSERVATIONS 645, 652, 659, 666, 673, 680 687, 694 701, 707 645, 652, 659, 666, 673, 680 687, 694 701, 707 694, 701, 707 694, 701, 707 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 367 1145 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 436 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91366 M 86 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 80 VENTRAL-ABDOMINAL-LEFT WEEK 74 4 53 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB MOUTH NORMAL NO REMARKABLE OBSERVATIONS 596, 600 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505 554C, 561C, 568C, 575C, 582C, 589C , 596C, 600C 512C, 519C, 526C, 533C, 54 0C, 547C , 554C, 561C, 568C, 575C , 582C 589C, 596C, 600C 229, 232, 239, 246, 253, 260, 267, 274, 281, 288 , 295, 302 , 309 316, 323, 330, 337, 344, 351, 358, 365, 367 229, 232 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 2O 0O4A, 2O i1l1, 2O 118o, 2o 2o 5c 1146 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 437 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91368 4 53 NORMAL NO REMARKABLE OBSERVATIONS C91369 M 74 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 63 AXILLARY REGION-RIGHT WEEK 45 VENTRAL-THORACIC-LEFT WEEK 67 3 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183 , 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365 , 367 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183 , 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274 , 281 , 288 295, 302 435C, 442C , 449C, 456C, 463C, 470C , 477C, 484C, 491C, 498C , 505C 512C 309, 316C, 323C, 330C, 337C, 344C, 351C, 358C , 365C, 372C, 379C 386C, 393C , 400C, 407C, 414C, 421C , 428C, 435C, 442C, 449C , 456C 463C, 470C , 477C, 484C, 491C, 498C , 505C, 512C 463, 470, 477,. 484C, 491C, 498C, 505C, 512C 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 94 1147 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 438 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91371 4 53 NORMAL NO REMARKABLE OBSERVATIONS C91372 T MASS(ES) AXILLARY REGION-RIGHT WEEK 52 105 APPEARANCE MALOCCLUSION DISCHARGE NASAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351 358, 365, 367 218, 225, 232, 239 , 246, 253, 260, 267 , 274, 281, 288 309, 316, 323, 330 , 337, 344, 351, 358 , 365, 372, 379 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470 491, 498, 505, 512 , 519, 526, 533, 540 , 547, 554, 561 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652 673, 680, 687,. 694 , 701, 708, 715, 722 , 729, 731 295, 302 386, 393 477, 484 568, 575 659, 666 218 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211 1148 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 439 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91373 M 69 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS M 98 APPEARANCE MALOCCLUSION THIN DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES 477 477 477 470, 477 477 477 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 421, 428, 435, 442 , 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526, 533 , 540, 547, 554, 561 , 568 677, 680 673, 677, 680 677 680 1149 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 440 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91374 M 98 EYES RED DISCHARGE EYE-RIGHT SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91375 M WART LIKE LESION(S) TAIL-PROXIMAL WEEK 88 83 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES EYES RED DISCHARGE EYES DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 673, 677, 680 677, 680 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 575, 582, 589 , 596, 603 610, 617, 624, 631, 638, 645, 652, 659 , 666, 673, 677, 680 578-579 578-579 579 575, 578-579 575, 578-579 1150 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 441 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91375 M 83 NORMAL NO REMARKABLE OBSERVATIONS C91376 T NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 100 INGUINAL REGION-LEFT WEEK 105 WART LIKE LESION(S) DORSAL WEEK 89 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610 694C, 701C , 708C, 715C, 722C, 729- 730C 729-730C 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722 1151 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 442 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91377 D 71 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 C91378 T 105 APPEARANCE MALOCCLUSION THIN DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 449C, 456C, 463C, 470C, 477C, 484C, 491C, 497C 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 663 663, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 663, 666, 673, 680 666, 673, 680 663, 666, 673, 680 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 1152 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 443 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91378 T 105 NORMAL NO REMARKABLE OBSERVATIONS C91379 3 C91380 T 14 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE CONVULSIONS MALOCCLUSION DISCHARGE GENITAL MUCOID EYES DISCOLORED/LIGHT RED EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 94 498, 505 253, 260, 267, 330 , 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491, 498, 505, 512 , 519, 526, 533,. 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 701, 708, 715, 719 , 722, 729- 730 372, 379, 386 645, 652, 659, 666 , 673, 680, 687,. 694 , 701, 708, 715, 719 , 722 729- 730 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 274 , 281, 288, 295, 302 , 309 316, 323 1153 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 444 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F4 (CONTINUED FROM PREVIOUS PAGE) C91380 T 105 MASS(ES) AXILLARY REGION-LEFT WEEK 92 VENTRAL-ABDOMINAL-RIGHT WEEK 100 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 638C, 645C, 652C, 659C, 666C , 673C,. 680C, 687C, 694C, 701C, 708C 715C, 719C, 722C, 729-730C 694C, 701C, 708C, 715C, 719C , 722C, 729-730C 1154 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) 87 AXILLARY REGION-RIGHT WEEK 85 85 MASS(ES) 87 VENTRAL-ABDOMINAL WEEK 85 71 MASS(ES) 78 VENTRAL-ABDOMINAL-LEFT WEEK 71 79 MASS(ES) 82 VENTRAL-ABDOMINAL-LEFT WEEK 71 83 MASS(ES) 85 VENTRAL-ABDOMINAL-LEFT WEEK 71 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 71 87 MASS(ES) 87 VENTRAL-ABDOMINAL-LEFT WEEK 71 48 MASS(ES) 54 HEAD-MAXILLARY-RIGHT WEEK 48 25 X 30MM 45 X 45MM 20 X 20MM 25 X 25MM 30 X 25MM 30 X 25MM 40 X 40MM 10 X 15MM 1155 Covance 6329-212 3M T-6316.1 PAGE: 445 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C91314 C91315 C91321 C91323 F4 421 61 TO 512 74 MASS(ES) VENTRAL -CERVICAL-RIGHT WEEK 61 F4 687 99 MASS(ES) TO 731 105 DORSAL WEEK 99 715 103 MASS(ES) DORSAL WEEK 103 722 104 MASS(ES) TO 731 105 DORSAL WEEK 103 F4 624 90 TO 631 91 MASS(ES) DORSAL WEEK 90 638 92 TO 645 93 MASS(ES) DORSAL WEEK 90 652 94 TO 654 94 MASS(ES) DORSAL WEEK 90 F4 351 51 TO 367 53 MASS(ES) VENTRAL -ABDOMINAL-RIGHT WEEK 51 35 X 35MM 10 X 10MM 30 X 30MM SIDE RIGHT 50 X 40MM SIDE RIGHT 30 X 30 40 X 45MM 20 X 20MM 10 X 10MM 1156 Covance 6329-212 3M T-6316.1 PAGE: 446 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91327 F4 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 589 85 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 638 92 TO 652 94 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 666 96 TO 673 97 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 680 98 MASS(ES) TO 722 104 VENTRAL-ABDOMINAL-LEFT WEEK 84 729 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 731 105 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 84 10 X 10MM 15 X 15MM 15 X 25MM 20 X 25MM 30 X 25MM 30 X 30MM 30 X 30MM 30 X 30MM 1157 Covance 6329-212 3M T-6316.1 PAGE: 447 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 68 MASS(ES) 69 DORSAL WEEK 68 70 MASS(ES) 74 DORSAL WEEK 68 75 MASS(ES) 84 DORSAL WEEK 68 85 MASS(ES) 85 DORSAL WEEK 68 80 MASS(ES) 82 DORSAL-CERVICAL-LEFT WEEK 80 83 MASS(ES) 85 DORSAL-CERVICAL-LEFT WEEK 80 85 MASS(ES) 85 VENTRAL-ABDOMINAL-LEFT WEEK 84 85 MASS(ES) 85 VENTRAL-ABDOMINAL-LEFT WEEK 85 10 X 10MM 20 X 20MM 25 X 25MM 35 X 35MM 20 X 20MM 30 X 30MM 15 X 15MM ANTERIOR 20 X 20MM POSTERIOR 1158 Covance 6329-212 3M T-6316.1 PAGE: 448 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 85 MASS(ES) 85 VENTRAL-ABDOMINAL-RIGHT WEEK 85 56 MASS(ES) VENTRAL-THORACIC WEEK 54 57 MASS(ES) 59 VENTRAL-THORACIC WEEK 54 60 MASS(ES) VENTRAL-THORACIC WEEK 54 61 MASS(ES) VENTRAL-THORACIC WEEK 54 62 MASS(ES) 63 VENTRAL-THORACIC WEEK 54 64 MASS(ES) 74 VENTRAL-THORACIC WEEK 54 75 MASS(ES) 85 VENTRAL-THORACIC WEEK 54 20 X 20 MM 10 X 10MM 25 X 25MM 30 X 25MM 40 X 30MM 40 X 35MM 45 X 40MM 50 X 45MM 1159 Covance 6329-212 3M T-6316.1 PAGE: 449 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91334 C91336 F4 610 88 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 86 638 92 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 86 673 97 TO 676 97 MASS(ES) AXILLARY REGION-LEFT WEEK 86 680 98 MASS(ES) TO 705 101 AXILLARY REGION-LEFT WEEK 86 225 33 MASS(ES) VENTRAL-ABDOMINAL WEEK 33 673 97 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 97 687 99 MASS(ES) TO 705 101 VENTRAL-ABDOMINAL-RIGHT WEEK 97 F4 638 92 TO 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 88 10 X 10MM 20 X 20MM 25 X 25MM 30 X 30MM 10 X 10MM 45 X 60MM 60 X 60MM 10 X 10MM 1160 Covance 6329-212 3M T-6316.1 PAGE: 450 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91336 C91340 C91341 F4 673 97 MASS(ES) TO 730 105 VENTRAL-ABDOMINAL-RIGHT WEEK 88 F4 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 589 85 MASS(ES) TO 731 105 VENTRAL-ABDOMINAL-LEFT WEEK 67 463 67 TO 484 70 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 67 491 71 TO 540 78 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 67 547 79 TO 568 82 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 67 F4 519 75 TO 540 78 MASS(ES) VENTRAL-CERVICAL WEEK 73 547 79 TO 568 82 MASS(ES) VENTRAL-CERVICAL WEEK 73 10 X 15MM 50 X 40MM APPEARS TO HAVE MIGRATED FROM VENTRAL THORACIC 60 X 55MM APPEARS TO HAVE MIGRATED FROM VENTRAL THORACIC 40 X 20MM 40 X 30MM 50 X 40MM 15 X 15MM POSTERIOR 30 X 30MM POSTERIOR 1161 Covance 6329-212 3M T-6316.1 PAGE: 451 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F4 575 83 MASS(ES) VENTRAL-CERVICAL WEEK 73 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 73 589 85 TO 610 CO CO MASS(ES) VENTRAL-CERVICAL WEEK 73 617 89 TO 631 91 MASS(ES) VENTRAL-CERVICAL WEEK 73 638 92 TO 666 96 MASS(ES) VENTRAL-CERVICAL WEEK 73 673 97 MASS(ES) TO 730 105 VENTRAL-CERVICAL WEEK 73 519 75 TO 568 82 MASS(ES) VENTRAL-CERVICAL WEEK 75 575 83 TO 582 84 MASS(ES) VENTRAL-CERVICAL WEEK 75 40 X 40MM POSTERIOR 45 X 45MM POSTERIOR 55 X 55MM POSTERIOR 50 X 40MM 55 X 40MM 60 X 40MM ANTERIOR 10 X 10MM ANTERIOR 1162 Covance 6329-212 3M T-6316.1 PAGE: 452 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91341 C91344 C91351 F4 589 85 TO 610 88 MASS(ES) VENTRAL-CERVICAL WEEK 75 F4 652 94 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 94 673 97 MASS(ES) TO 715 103 VENTRAL-ABDOMINAL-RIGHT WEEK 94 F4 582 84 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 610 88 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 638 92 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 15 X 15MM ANTERIOR 30 X 30MM 30 X 40MM 40 X 50MM 60 X 50MM 10 X 10MM 15 X 15MM 30 X 30MM 1163 Covance 6329-212 3M T-6316.1 PAGE: 453 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 84 61 MASS(ES) 65 VENTRAL-CERVICAL-LEFT WEEK 61 66 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 61 67 MASS(ES) 75 VENTRAL-CERVICAL-LEFT WEEK 61 85 MASS(ES) 87 AXILLARY REGION-LEFT WEEK 85 88 MASS(ES) 91 AXILLARY REGION-LEFT WEEK 85 92 MASS(ES) 96 AXILLARY REGION-LEFT WEEK 85 15 X 15MM 15 X 15MM 30 X 35MM 50 X 35MM 50 X 40MM 30 X 25MM 40 X 35MM 60 X 50MM 1164 Covance 6329-212 3M T-6316.1 PAGE: 454 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91362 C91366 F4 673 97 MASS(ES) TO 722 104 AXILLARY REGION-LEFT WEEK 85 729 105 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 85 617 89 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 638 92 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 652 94 TO 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 89 680 98 MASS(ES) TO 732 105 AXILLARY REGION-RIGHT WEEK 89 F4 554 80 TO 568 82 MASS(ES) INGUINAL REGION-LEFT WEEK 80 575 83 MASS(ES) INGUINAL REGION-LEFT WEEK 80 70 X 70MM 90 X 65MM 10 X 10MM 15 X 20MM 40 X 30MM 50 X 40MM 10 X 10MM 20 X 20MM 1165 Covance 6329-212 3M T-6316.1 PAGE: 455 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 84 MASS(ES) 86 INGUINAL REGION-LEFT WEEK 80 74 MASS(ES) 75 VENTRAL-ABDOMINAL-LEFT WEEK 74 76 MASS(ES) 79 VENTRAL-ABDOMINAL-LEFT WEEK 74 80 MASS(ES) 82 VENTRAL-ABDOMINAL-LEFT WEEK 74 83 MASS(ES) 86 VENTRAL-ABDOMINAL-LEFT WEEK 74 63 MASS(ES) 69 AXILLARY REGION-LEFT WEEK 63 70 MASS(ES) 74 AXILLARY REGION-LEFT WEEK 63 46 MASS(ES) 47 AXILLARY REGION-RIGHT WEEK 45 35 X 30MM 15 X 15MM 20 X 25MM 40 X 30MM 30 X 30MM 20 X 20MM 20 X 25MM 20 X 25MM 1166 Covance 6329-212 3M T-6316.1 PAGE: 456 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 48 MASS(ES) 53 AXILLARY REGION-RIGHT WEEK 45 54 MASS(ES) 58 AXILLARY REGION-RIGHT WEEK 45 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 61 MASS(ES) AXILLARY REGION-RIGHT WEEK 45 62 MASS(ES) 69 AXILLARY REGION-RIGHT WEEK 45 70 MASS(ES) 71 AXILLARY REGION-RIGHT WEEK 45 72 MASS(ES) 74 AXILLARY REGION-RIGHT WEEK 45 35 X 30MM 35 X 40MM 40 X 40MM 45 X 45MM 45 X 50MM 65 X 60MM 65 X 65MM 55 X 55MM 1167 Covance 6329-212 3M T-6316.1 PAGE: 457 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91369 C91376 C91377 F4 484 70 TO 512 74 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 67 F4 694 100 MASS(ES) TO 730 105 AXILLARY REGION-LEFT WEEK 100 729 105 MASS(ES) TO 730 105 INGUINAL REGION-LEFT WEEK 105 F4 295 43 TO 302 44 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 309 45 TO 344 50 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 351 51 TO 365 53 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 372 54 TO 400 58 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 20 X 20MM 10 X 10MM THREE MASSES UNDER 10MM 10 X 10MM 25 X 20MM 40 X 30MM 40 X 50MM 55 X 55MM 1168 Covance 6329-212 3M T-6316.1 PAGE: 458 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91377 C91380 F4 414 60 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 421 61 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 428 62 TO 497 71 MASS(ES) VENTRAL-CERVICAL-RIGHT WEEK 43 F4 638 92 TO 666 96 MASS(ES) AXILLARY REGION-LEFT WEEK 92 673 97 MASS(ES) TO 730 105 AXILLARY REGION-LEFT WEEK 92 694 100 MASS(ES) TO 730 105 VENTRAL-ABDOMINAL-RIGHT WEEK 100 60 X 60MM 70 X 65MM 90 X 85MM 25 X 30MM 35 X 30MM 35 X 35MM Covance 6329-212 3M T-6316.1 PAGE: 459 1169 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 460 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91381 6 C91382 6 C91383 6 C91384 6 C91385 6 C91386 6 C91387 6 C91388 6 C91389 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1170 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 461 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91390 6 C91391 6 C91392 6 C91393 2 C91394 2 C91395 6 C91396 6 C91397 6 C91398 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1171 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 462 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91399 6 C91400 6 C91401 6 C91402 6 C91403 6 C91404 2 C91405 6 C91406 6 C91407 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1172 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 463 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91408 6 C91409 6 C91410 6 C91411 6 C91412 6 C91413 6 C91414 6 C91415 2 C91416 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50 1173 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 464 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91417 6 C91418 6 C91419 6 C91420 6 C91421 6 C91422 6 C91423 1 C91424 6 C91425 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1174 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 465 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91426 6 C91427 1 C91428 6 C91429 6 C91430 1 C91431 6 C91432 6 C91433 6 C91434 6 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1175 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 466 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91435 6 C91436 6 C91437 6 C91438 2 C91439 6 C91440 6 C91441 6 C91442 6 C91443 1 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50, 54 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 1176 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 467 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F5 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91444 6 C91445 6 C91446 6 C91447 6 C91448 1 C91449 6 C91450 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 24 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1177 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 468 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91451 U 105 NORMAL NO REMARKABLE OBSERVATIONS C91452 M ASS(ES) AXILLARY REGION-RIGHT WEEK 92 INGUINAL REGION-LEFT WEEK 98 74 APPEARANCE THIN SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582,. 589, 596, 603, 610, 617 , 624, 631 638C, 645C , 652C, 659C, 666C, 673C , 680C, 68 7C, 694C, 701C , 708C 715C, 722C', 72 9C, 732C 680, 687, 694, 701, 708C , 715C , 722C, 729C, 732C 512 512 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295C, 302C, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C 1178 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 469 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91452 M 74 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 C91453 U 105 APPEARANCE THIN DISCHARGE NASAL RED IN COLOR SKIN & PELAGE SORE/SCAB PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 44 9C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 715, 722, 729, 732 729, 732 680, 687, 694,. 701, 708, 715, 722, 729 , 732 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673 1179 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 470 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS M 83 EXCRETION NO FECES RESPIRATION AUDIBLE LABORED SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT DORSAL-CERVICAL HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS C91455 U NORMAL NO REMARKABLE OBSERVATIONS 566, 568, 575, 578 578 578 578 566, 568, 575, 578 330, 337, 344, 351, 358, 365, 372, 379 , 386, 393, 400, 407 , 414 421, 428, 435,. 442, 449, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526,. 533, 540, 547, 566, 568 , 575, 578 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 554, 561 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 708, 715 , 722 729, 732 1180 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 471 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91455 U 105 MASS(ES) INGUINAL REGION-LEFT WEEK 92 C91456 M 77 APPEARANCE MALOCCLUSION EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C 484, 491, 498, 505, 512, 519, 526, 533 533 533 533 533 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477 1181 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 472 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91457 M 96 BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS C91458 M MASS(ES) INGUINAL REGION-RIGHT WEEK 84 63 APPEARANCE PROLAPSED VAGINA DISCHARGE GENITAL RED IN COLOR 667 667 667 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183 , 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274 , 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358, 365 , 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449, 456 , 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540, 547 , 554 , 561 568, 575 582, 589, 596, 603 , 610, 617, 624, 631C, 638C , 645C, 652C, 659C 666- 667C 435 428 1182 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 473 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91458 M 63 NORMAL NO REMARKABLE OBSERVATIONS C91459 U MASS(ES) VENTRAL-ABDOMINAL WEEK 62 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 102 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421 428, 435 267, 274, 281, 288 , 295, 302, 309, 316 , 323, 330, 337, 344 , 351 358, 365, 372, 379 , 386, 393, 400, 407 , 414, 421, 428, 435 , 442 449, 456, 463, 470 , 477, 484, 491, 498 , 505, 512, 519, 526 , 533 540, 547, 554, 561 , 568, 575, 582, 589 , 596, 603, 610, 617 , 624 631, 638, 645, 652 , 659, 666, 673, 680 , 687, 694, 701, 708 , 715 722, 729, 732 638, 645, 652, 659 , 666, 673, 680, 687 , 694, 701 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260 708, 715, 722, 729C, 732C 1183 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 474 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91460 D 62 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL THIN DISCHARGE GENITAL RED IN COLOR EXCRETION NONFORMED FECES SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 59 421, 428-429 400, 407, 414, 421, 428-429 372, 379, 386 428-429 421, 428-429 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 393 407C, 414C, 421C, 428-429C 1184 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 475 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91461 M 86 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN SKIN & PELAGE SORE/SCAB DORSAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 VENTRAL-ABDOMINAL-RIGHT WEEK 84 596, 600 43, 50, 57, 64, 71, 78, 85 600 463, 470, 477, 484 , 491, 498, 505, 509, 512, 519, 526, 533, 540 547, 554, 561, 568 , 575, 582, 589, 596, 600 582, 589, 596, 600 1, 8,r 15, 22, 29, 36, 92, 99, 106, 113, 120, 127, 134, 141, 148 155, 162, 169, 176 , 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253, 260, 267 , 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 509C, 512C, 519C, 526C, 533C, 54 0C, 547C, 554C, 561C, 568C 575C, 582C, 58 9C, 596C, 600C 582, 589, 596, 600 1185 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 476 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91462 U 105 NORMAL NO REMARKABLE OBSERVATIONS C91463 M 87 BEHAVIOR HYPOACTIVE RECUMBENT LATERAL EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 71 VENTRAL-ABDOMINAL-RIGHT WEEK 66 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 608 608 596, 608 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183,. 190, 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365,. 372, 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 491, 498, 505,. 512, 519, 526, 533, 540, 547, 554C, 561C, 56 8C 575C, 582C , 589C, 596C, 603C, 608C 456C, 463C , 470C, 477C, 484C, 491C , 498C , 505C, 512C, 519C, 526C 1186 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 477 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91463 M 87 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 C91464 M 74 APPEARANCE THIN EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 35 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 608C 498, 505, 512 505 498 498, 505 498, 505, 512 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232 239, 246, 253, 260C, 267C, 274C, 281C, 288C, 295C, 302C, 309C 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C, 379C, 386C 393C, 400C, 407C, 414C, 421C, 428C, 435C, 442C, 44 9C, 456C, 463C 470C, 477C, 484C, 491C, 498C, 505C, 512C 1187 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 478 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91465 M 90 APPEARANCE DEHYDRATED MALOCCLUSION SWOLLEN NOSE THIN EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91466 M 93 BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES 617, 624 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624 134 582, 589, 596, 603, 610, 617, 624 547, 554, 561,. 568, 575 134, 141 554, 561, 568,. 575, 582, 589, 596, 603 , 610, 617, 624 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 148, 155, 162, 169, 176 , 183, 190, 197, 204 , 211 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288, 295 , 302 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379 642, 645-647 647 642, 645 1188 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 479 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91466 M 93 EXCRETION NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB PAW-HIND-LEFT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 87 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 646-647 642, 645-647 642, 645-647 631, 638, 642, 645-647 647 1, 8, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596 603, 610, 617, 624, 631 1189 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 480 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91467 U 105 SKIN & PELAGE ROUGH HAIR COAT SCALY SKIN TAIL SORE/SCAB LIMBS-HIND PAW-HIND-LEFT PAWS-HIND TAIL NORMAL NO REMARKABLE OBSERVATIONS C91468 D 64 APPEARANCE MALOCCLUSION THIN NORMAL NO REMARKABLE OBSERVATIONS 358, 365 456, 463, 470, 477 , 484, 491, 498, 505 , 512, 519, 526, 533 , 540 547, 554, 561, 568 , 575, 582, 589, 596 , 603, 610, 617, 624 , 631 638, 645, 652, 659 , 666, 673, 680, 687 , 694, 701, 708, 715 , 722 729, 732 673, 680, 687,. 694 , 701, 708, 715, 722 , 729, 732 617, 624, 631, 638 , 645, 652, 659, 666 211, 218, 225, 232 197, 204, 211, 218 , 225, 232, 239, 246 , 253, 260, 267, 274 , 281 288, 295, 302, 309 , 316, 323, 330, 337 , 344 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 351 372, 379, 386, 393 , 400, 407, 414, 421 , 428, 435, 442, 449 106, 197, 281, 372, 213, 113, 204, 288, 379, 218, 120, 211, 295, 386, 225, 127 , 134, 213 , 218, 302 , 309, 393 , 400, 232 , 239, 141, 225, 316, 407, 246, 148, 232, 323, 414, 253, 155 , 162, 239 , 246, 330 , 337, 421 , 428, 260 169, 253, 344, 435, 176, 260, 351, 442 183 , 190 267 , 274 358 , 365 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99 1190 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 481 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91469 D 72 EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE PERINEAL AREA ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91470 M 69 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE EXCRETION FEW FECES NO FECES 498, 500 498, 500 372, 379, 386, 393 , 400, 407, 414, 421 , 428, 498, 500 498, 500 498, 500 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 435 , 442 449, 456, 463, 470 , 477, 484, 491 477 477 477 477 477 1191 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 482 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91470 M 69 EYES PALE EYES RED DISCHARGE EYES RESPIRATION AUDIBLE SKIN & PELAGE BROWN HAIR COAT GENERALIZED COLD TO TOUCH ENTIRE BODY RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477 477 477 477 477 477 477 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 1192 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 483 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91471 M 97 APPEARANCE THIN EXCRETION NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 44 WEEK 74 VENTRAL-ABDOMINAL-RIGHT WEEK 51 VENTRAL-CERVICAL-LEFT WEEK 48 673, 675 675 673, 675 526, 533 638, 645, 652, 659 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 337, 344, 393 , 400, 407, 414, 421 , 428, 435, 442, 449 , 456 463, 470, 477, 484 , 491, 498, 505, 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603 , 610, 617, 624, 631 , 666 302, 309, 316, 323, 330 512C, 519C 351, 358, 365, 372, 379, 386 330 1193 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 484 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91472 M 98 NORMAL NO REMARKABLE OBSERVATIONS C91473 U MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 87 VENTRAL-CERVICAL-LEFT WEEK 87 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85 , 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 , 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 , 365,. 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 , 456 463C, 470C , 477C, 484C, 491C, 498C , 505C, 512C, 519C, 526C, 533C 54 0C, 547C , 554C, 561C, 568C, 575C , 582C, 58 9C, 596C, 603C, 610C 617C, 624C , 631C, 638C, 641C, 645C , 652C, 65 9C, 666C, 673C, 680C 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85 , 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 , 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 , 365,. 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 , 456,. 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547,. 554, 561 568, 575, 582, 589, 596 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 , 673, 680, 687 694, 701, 708C , 715C, 722C, 729C, 732C 603, 610, 617, 624, 631, 638C, 645C, 652C, 659C, 666C, 673C 680C, 687C', 694C, 701C 1194 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 485 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91474 M 93 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN BEHAVIOR HYPOACTIVE EXCRETION NO FECES SKIN & PELAGE ALOPECIA ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 638, 645 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645 617, 624, 631, 638, 645 638, 645 183 617, 624, 631 358, 365, 372 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113 1195 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 486 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91475 M 100 APPEARANCE SWOLLEN VENTRAL-ABDOMINAL DISCHARGE GENITAL RED IN COLOR EXCRETION NO FECES NONFORMED FECES EYES CLEAR DISCHARGE EYE-RIGHT RED DISCHARGE EYE-RIGHT SKIN & PELAGE ALOPECIA BROWN HAIR COAT VENTRAL-ABDOMINAL PALE ENTIRE BODY RED HAIR COAT PERINEAL AREA ROUGH HAIR COAT SORE/SCAB LIMBS-HIND PAW-HIND-RIGHT 582, 589, 596 288, 358, 365, 372, 379, 386, 547, 554, 582 694, 697 603, 610 316, 323, 491, 498, 505, 512, 519, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 697 295, 302, 309 617, 624 631, 638, 645, 652 694 358, 365 372, 379, 386 697 358, 365 372 673, 680 687, 694, 697 421, 428 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505 512, 519 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596 1196 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 487 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91475 M 100 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91476 M 80 APPEARANCE THIN BEHAVIOR HYPOACTIVE DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES RESPIRATION AUDIBLE IRREGULAR DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 603, 610, 617, 624 , 631, 638, 645, 652 , 659, 666 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 330 337, 344, 351, 393 , 400, 407, 414 547 557 547 533, 540 557 533, 540, 547, 554, 557 557 557 1197 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 488 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91476 M 80 SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91477 M 86 APPEARANCE THIN BEHAVIOR HYPOACTIVE EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 554, 557 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 547, 554, 561, 568, 575, 582, 589, 596 589, 596 582, 589, 596 582, 589, 596 582, 589, 596 1, 8,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309 1198 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 489 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91477 M 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 C91478 D 85 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB DORSAL-THORACIC-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 78 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 316C, 323C , 330C, 337C, 344C, 351C , 358C, 365C, 372C, 379C , 386C 393C, 400C , 407C, 414C, 421C, 428C , 435C, 442C, 44 9C, 456C , 463C 470C, 477C , 484C, 491C, 498C, 505C , 512C, 519C, 526C, 533C , 540C 547C, 554C , 561C, 568C, 575C, 582C , 589C, 596C 582, 589, 593 547, 554, 561,. 568, 575, 582, 589, 593 1, 8,. 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491,. 498, 505, 512, 519, 526, 533 54 0C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 593C 1199 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 490 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91479 U 105 EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS C91480 U 105 NORMAL NO REMARKABLE OBSERVATIONS C91481 M 73 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC 428, 435, 442, 449 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 456, 463 , 470, 477, 484, 491 , 498 505, 512, 519,. 526, 533, 540, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617, 624, 631, 638, 645 , 652, 659, 666, 673 , 680 687, 694, 701,. 708, 715, 722, 729, 732 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 509 509 509 1200 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 491 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91481 M 73 EXCRETION FEW FECES SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL RED HAIR COAT HEAD-ENTIRE SORE/SCAB VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91482 M MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 93 APPEARANCE DEHYDRATED HEAD TILT RIGHT HUNCHED THIN DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 491 509 470 509 470, 477 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 509C 617, 624, 631, 638 638, 642, 645 642, 645 582, 589, 596, 603, 609-610, 617, 624, 631, 638, 642, 645 1201 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 492 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91482 M 93 BEHAVIOR ATAXIC EXCRETION FEW FECES EYES PALE EYES RED DISCHARGE EYES SKIN & PELAGE ALOPECIA ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) MOUTH WEEK 56 VENTRAL-ABDOMINAL-RIGHT WEEK 72 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 642, 645 617, 624 642, 645 575, 582, 589, 596 , 603, 609- 610, 617, 624, 631, 638, 642, 645 617, 624, 631 617, 624, 631, 638 , 642, 645 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421 , 428, 435, 442, 449 , 456, 463, 470, 477 , 484 491 386, 393 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C, 561C, 568C 575C, 582C, 58 9C, 596C, 603C, 609-610C, 617C, 624C, 631C, 638C 642C, 645C 1202 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 493 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91483 D 96 APPEARANCE MALOCCLUSION SWOLLEN LIMB-HIND-RIGHT EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C91484 M 71 APPEARANCE HUNCHED MALOCCLUSION THIN BEHAVIOR HYPOACTIVE SKIN & PELAGE COLD TO TOUCH ENTIRE BODY 393, 400, 407 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 624, 631, 638, 645, 652, 659, 666 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 488, 141, 232, 323, 414, 4a 7n 7n, 491 148, 239, 330, 421, 4A 8O4A, 155, 246, 337, 428, 4A 8O8O, 162, 253, 344, 435, 4a 9m1 169, 260, 351, 442, 176, 267, 358, 449, 183, 274, 365, 456, 190, 281, 372, 463, 197, 288, 379, 470, 204, 295, 386, 477, 211, 302, 393, 484, 218, 309, 400, 488, 225 316 407 491 488, 491 491 1203 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 494 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91484 M 71 SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS U 105 APPEARANCE MALOCCLUSION DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 488, 491 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 99, 106, 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 190, 197, 204, 211 , 218, 225, 232, 239 , 246, 253, 260, 267 , 274 281, 288, 295, 302 , 309, 316, 323, 330 , 337, 344, 351, 358 , 365 372, 379, 386, 393 , 400, 407, 414, 421 , 428, 435, 442, 449 , 456 463, 470, 477, 484 , 491, 498, 505, 512 , 519, 526, 533, 540 , 547 554, 561, 568, 575 , 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 659, 666 , 673, 680, 687,. 694 , 701, 708, 715, 722 , 729 732 281, 288 1, 8, 15, 22, 29, 36, 43, 57, 64, 71, 78, 85, 92 1204 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 495 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91486 M 92 APPEARANCE BENT TAIL DEHYDRATED MISSING TAIL-PROXIMAL SWOLLEN MOUTH THIN BEHAVIOR ATAXIC HYPOACTIVE EXCRETION FEW FECES EYES OPAQUE EYE-RIGHT 134 617, 624, 631, 638 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400, 407 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 547, 554, 561, 564, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 640 617, 624, 631, 638, 640 603, 610, 617, 624, 631, 638, 640 610, 617, 624, 631, 638, 640 640 564, 568, 575, 617, 624, 631, 638, 640 29, 36, 43 , 50,r 57, 64, 71, 78 , 85,r 92, 99, 106, 113, 120, 127 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 564, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 640 1205 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 496 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91486 M 92 SKIN & PELAGE PALE ENTIRE BODY YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C91487 U 105 NORMAL NO REMARKABLE OBSERVATIONS C91488 U 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 564, 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 640 610, 617, 624, 631, 638, 640 1, 8, 15, 22 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729, 732 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729, 732 554 , 561, 568, 575 8, 15, 22, 29, 36, 43, 50, 57,, 64, 71, 78, 85, 92, 99, 106, 113 120 , 127, 134, 141,. 148, 155, 162, 169, 176 , 183, 190, 197, 204 211 , 218, 225, 232,. 239, 246, 253, 260, 267 , 274, 281, 288, 295 302 , 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379, 386 1206 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 497 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91488 U 105 NORMAL NO REMARKABLE OBSERVATIONS C91489 D 81 DISCHARGE NASAL RED IN COLOR SKIN & PELAGE ALOPECIA NORMAL NO REMARKABLE OBSERVATIONS C91490 M MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 79 83 APPEARANCE MALOCCLUSION SWOLLEN MOUTH EYES RED DISCHARGE EYE-LEFT DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 120 120 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 127, 134, 141 , 148, 155, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540 547C, 554C, 561C 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554, 561, 565, 568, 575 428 358, 365, 372 1207 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 498 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F6 (CONTINUED FROM PREVIOUS PAGE) C91490 M 83 SKIN & PELAGE BROWN HAIR COAT GENERALIZED BLACK SKIN VENTRAL-THORACIC-RIGHT ROUGH HAIR COAT SORE/SCAB MOUTH VENTRAL-ABDOMINAL VENTRAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 68 VENTRAL-THORACIC-RIGHT WEEK 75 DOSE: 100 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 456, 463 540, 547 358, 365, 372, 379, 386, 393, 400, 407 435, 442, 449,. 540, 547 547, 554, 561, 565, 568, 575 565, 568, 575 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 414, 421 470C, 477C, 484C, 491C 519C, 526C, 533C, 540C, 547C, 554C, 561C, 565C, 568C, 575C 1208 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 92 MASS(ES) 96 AXILLARY REGION-RIGHT WEEK 92 97 MASS(ES) 101 AXILLARY REGION-RIGHT WEEK 92 102 MASS(ES) 104 AXILLARY REGION-RIGHT WEEK 92 105 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 92 102 MASS(ES) INGUINAL REGION-LEFT WEEK 98 103 MASS(ES) 104 INGUINAL REGION-LEFT WEEK 98 105 MASS(ES) 105 INGUINAL REGION-LEFT WEEK 98 43 MASS(ES) 44 VENTRAL-CERVICAL-LEFT WEEK 43 10 X 10MM 20 X 20MM 30 X 25MM 30 X 30MM 10 X 10MM 15 X 15MM 15 X 20MM 15 X 15MM 1209 Covance 6329-212 3M T-6316.1 PAGE: 499 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91452 C91455 F6 309 45 TO 323 47 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 330 48 TO 379 55 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 386 56 TO 407 59 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 414 60 TO 477 69 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 484 70 TO 491 71 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 498 72 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 505 73 TO 512 74 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 43 F6 638 92 MASS(ES) TO 701 101 INGUINAL REGION-LEFT WEEK 92 20 X 20MM 25 X2 5MM 35 X 25MM 40 X 30MM 40 X 40MM 70 X 60MM 80 X 70MM 10 X 10MM 1210 Covance 6329-212 3M T-6316.1 PAGE: 500 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91457 C91459 C91460 C91461 C91463 F6 631 91 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 638 92 TO 667 96 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 F6 729 105 MASS(ES) TO 732 105 VENTRAL-ABDOMINAL WEEK 102 F6 407 59 TO 429 62 MASS(ES) VENTRAL-ABDOMINAL WEEK 59 F6 351 51 TO 600 86 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 F6 554 80 TO 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 71 575 83 TO 608 87 MASS(ES) AXILLARY REGION-LEFT WEEK 71 456 66 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 10 X 10MM 15 X 15MM 20 X 15MM 40 X 40MM 10 X 10MM 10 X 10MM 20 X 20MM 10 X 15MM 1211 Covance 6329-212 3M T-6316.1 PAGE: 501 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 67 MASS(ES) 69 VENTRAL-ABDOMINAL-RIGHT WEEK 66 70 MASS(ES) 71 VENTRAL-ABDOMINAL-RIGHT WEEK 66 72 MASS(ES) 84 VENTRAL-ABDOMINAL-RIGHT WEEK 66 85 MASS(ES) 87 VENTRAL-ABDOMINAL-RIGHT WEEK 66 38 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 39 MASS(ES) 41 VENTRAL-ABDOMINAL WEEK 35 42 MASS(ES) 59 VENTRAL-ABDOMINAL WEEK 35 60 MASS(ES) 71 VENTRAL-ABDOMINAL WEEK 35 20 X 30MM 25 X 40MM 25 X 25MM 25 X 30MM 15 X 10MM 15 X 10MM 25 X 20MM 30 X 25MM 1212 Covance 6329-212 3M T-6316.1 PAGE: 502 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 72 MASS(ES) 74 VENTRAL-ABDOMINAL WEEK 35 74 MASS(ES) 75 VENTRAL-ABDOMINAL WEEK 74 67 MASS(ES) 69 VENTRAL-ABDOMINAL-LEFT WEEK 67 70 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 71 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 72 MASS(ES) 74 VENTRAL-ABDOMINAL-LEFT WEEK 67 75 MASS(ES) 77 VENTRAL-ABDOMINAL-LEFT WEEK 67 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 40 X 40MM 10 X 10MM 30 X 30MM 30 X 40MM 40 X 50MM 50 X 60MM 55 X 75MM 75 X 75MM 1213 Covance 6329-212 3M T-6316.1 PAGE: 503 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C91472 C91473 C91477 F6 547 79 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 561 81 TO 568 82 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 575 83 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 589 85 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 638 92 TO 680 98 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 67 F6 708 102 MASS(ES) TO 732 105 AXILLARY REGION-LEFT WEEK 87 638 92 MASS(ES) TO 701 101 VENTRAL-CERVICAL-LEFT WEEK 87 F6 316 46 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 80 X 75MM 80 X 80MM 80 X 90MM 90 X 100MM 100 X 115MM 20 X 20MM 10 X 10MM 10 X 10MM 1214 Covance 6329-212 3M T-6316.1 PAGE: 504 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 48 MASS(ES) 50 VENTRAL-ABDOMINAL-LEFT WEEK 46 51 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 52 MASS(ES) 53 VENTRAL-ABDOMINAL-LEFT WEEK 46 54 MASS(ES) 59 VENTRAL-ABDOMINAL-LEFT WEEK 46 60 MASS(ES) 61 VENTRAL-ABDOMINAL-LEFT WEEK 46 62 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 63 MASS(ES) 74 VENTRAL-ABDOMINAL-LEFT WEEK 46 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 20 X 20MM 25 X 25MM 25 X 35MM 35 X 40MM 45 X 45MM 55 X 50MM 55 X 60MM 70 X 75MM 1215 Covance 6329-212 3M T-6316.1 PAGE: 505 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 76 MASS(ES) 77 VENTRAL-ABDOMINAL-LEFT WEEK 46 78 MASS(ES) 84 VENTRAL-ABDOMINAL-LEFT WEEK 46 85 MASS(ES) 86 VENTRAL-ABDOMINAL-LEFT WEEK 46 78 MASS(ES) AXILLARY REGION-LEFT WEEK 78 79 MASS(ES) 82 AXILLARY REGION-LEFT WEEK 78 83 MASS(ES) 84 AXILLARY REGION-LEFT WEEK 78 85 MASS(ES) 85 AXILLARY REGION-LEFT WEEK 78 61 MASS(ES) 62 VENTRAL-ABDOMINAL-LEFT WEEK 61 60 X 55MM 60 X 65MM 60 X 70MM 20 X 30MM 30 X 40MM 70 X 60MM 70 X 70MM 15 X 15MM 1216 Covance 6329-212 3M T-6316.1 PAGE: 506 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 63 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 64 MASS(ES) 65 VENTRAL-ABDOMINAL-LEFT WEEK 61 66 MASS(ES) 72 VENTRAL-ABDOMINAL-LEFT WEEK 61 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 73 MASS(ES) 73 VENTRAL-ABDOMINAL-LEFT WEEK 61 72 MASS(ES) 76 VENTRAL-ABDOMINAL-RIGHT WEEK 72 77 MASS(ES) 79 VENTRAL-ABDOMINAL-RIGHT WEEK 72 80 MASS(ES) 85 VENTRAL-ABDOMINAL-RIGHT WEEK 72 30 X 20MM 35 X 20MM 40 X 25MM 40 X 35MM 50 X 40MM 15 X 15MM 20 X 20MM 30 X 20MM 1217 Covance 6329-212 3M T-6316.1 PAGE: 507 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 86 MASS(ES) 91 VENTRAL-ABDOMINAL-RIGHT WEEK 72 92 MASS(ES) 93 VENTRAL-ABDOMINAL-RIGHT WEEK 72 79 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 79 80 MASS(ES) 81 VENTRAL-CERVICAL-LEFT WEEK 79 68 MASS(ES) 71 AXILLARY REGION-RIGHT WEEK 68 75 MASS(ES) 76 VENTRAL-THORACIC-RIGHT WEEK 75 77 MASS(ES) 78 VENTRAL-THORACIC-RIGHT WEEK 75 79 MASS(ES) 83 VENTRAL-THORACIC-RIGHT WEEK 75 30 X 30MM 30 X 35MM 20 X 10MM 20 X 15MM 20 X 20MM 20 X 20MM 50 X 50MM 20 X 30MM 1218 Covance 6329-212 3M T-6316.1 PAGE: 508 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 509 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91491 6 C91492 6 C91493 6 C91494 6 C91495 6 C91496 6 C91497 6 C91498 6 C91499 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1219 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 510 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91500 6 C91501 6 C91502 6 C91503 6 C91504 6 C91505 6 C91506 6 C91507 6 C91508 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1220 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 511 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91509 6 C91510 6 C91511 6 C91512 6 C91513 6 C91514 6 C91515 6 C91516 6 C91517 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1221 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 512 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91518 6 C91519 6 C91520 6 C91521 6 C91522 6 C91523 6 C91524 6 C91525 6 C91526 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1222 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 513 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F7 DOSE: 300 PPM DAYS 1-732 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C91527 6 C91528 6 C91529 6 C91530 6 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 8 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1, 8, 15, 22, 29, 36, 43, 50 1223 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-212 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e IV o f X II Page 1224 o f 4873 Covance 6329-212 3M T-6316.1 VOLUME IV OF XII CONTENTS Page A P P E N D I X 3 .......................................................................................................................................................................................1 2 2 6 I n d i v i d u a l B o d y W e i g h t D a t a ( g ) ...............................................................................................................................1 2 2 7 A P P E N D I X 4 .......................................................................................................................................................................................1 3 0 6 I n d i v i d u a l F o o d C o n s u m p t i o n D a t a ( g ) ................................................................................................................1 3 0 7 I n d i v i d u a l T e s t M a t e r i a l C o n s u m p t i o n D a t a ( m g / k g / d a y ) ..................................................................1 3 8 4 A P P E N D I X 5 .......................................................................................................................................................................................1 4 5 6 I n d i v i d u a l C l i n i c a l H e m a t o l o g y D a t a .....................................................................................................................1 4 5 7 I n d i v i d u a l C l i n i c a l C h e m i s t r y D a t a ..........................................................................................................................1 4 9 9 I n d i v i d u a l C l i n i c a l U r i n a l y s i s D a t a ..........................................................................................................................1 5 4 1 I n d i v i d u a l U r i n e C h e m i s t r y D a t a ...............................................................................................................................1 5 8 3 1225 APPENDIX 3 In d iv id u a l B o d y W e ig h t D a ta (g) Covance 6329-212 3M T-6316.1 1226 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 1 - 0 PPM C90711 267 313 357 386 402 431 446 467 C90712 253 304 345 376 403 425 445 462 C90713 280 329 372 413 444 475 495 515 C90714 251 292 326 363 389 415 440 456 C90715 310 350 393 423 438 459 478 492 C90716 235 282 326 358 388 411 435 450 C90717 278 319 362 403 428 448 481 496 C90718 252 292 339 380 410 433 462 478 C90719 223 265 303 337 C90720 280 332 380 427 462 496 519 539 C90721 288 338 387 437 487 533 569 594 C90722 246 288 325 354 378 403 414 433 C90723 271 328 383 418 453 482 511 530 C90724 257 300 339 372 398 424 448 459 C90725 258 303 345 379 C90726 243 288 336 373 404 435 455 459 C90727 293 345 398 445 480 514 540 563 C90728 250 294 332 373 397 430 454 474 C90729 277 323 369 408 441 470 494 502 C90730 277 318 355 392 415 439 467 483 C90731 258 311 367 410 443 477 503 524 C90732 295 349 391 442 C90733 226 278 324 363 397 429 451 473 C90734 307 366 412 459 494 524 556 576 C90735 264 306 347 373 400 423 437 449 C90736 263 302 340 370 399 429 453 474 C90737 254 298 341 378 412 444 470 486 C90738 247 293 329 355 384 405 427 444 C90739 263 308 350 380 408 432 456 466 C90740 250 289 335 368 396 423 442 466 C90741 248 292 327 358 386 412 433 449 Covance 6329-212 3M T-6316.1 PAGE: 1 WEEK WEEK 910 480 494 475 492 531 554 478 498 508 528 470 486 551 575 628 637 452 468 540 567 477 489 478 495 581 605 495 511 528 541 506 526 548 572 487 511 601 618 466 477 497 513 456 474 489 499 490 510 466 479 1227 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 1 - 0 PPM C90742 245 295 332 369 402 433 461 473 C90743 267 315 359 400 432 465 493 506 C90744 274 317 354 390 415 441 461 475 C90745 246 280 312 344 373 396 415 427 C90746 269 319 367 414 444 476 504 530 C90747 261 304 344 375 399 424 437 455 C90748 284 335 374 408 431 452 477 489 C90749 230 270 312 342 368 390 404 420 C90750 246 294 335 374 402 426 441 456 C90751 259 306 348 380 401 424 445 458 C90752 301 359 414 451 484 510 536 551 C90753 257 306 346 379 C90754 278 333 373 414 447 467 496 507 C90755 279 333 375 414 446 478 512 540 C90756 214 258 293 327 354 378 401 416 C90757 283 336 380 425 456 486 512 534 C90758 251 296 340 380 415 446 467 488 C90759 235 281 327 372 403 429 453 468 C90760 259 309 355 389 411 440 465 474 C90761 260 305 340 370 403 428 451 466 C90762 229 277 318 346 374 402 424 440 C90763 289 344 384 427 464 492 515 529 C90764 255 300 341 380 407 429 446 459 C90765 273 332 382 423 462 490 516 540 C90766 262 310 350 388 405 432 463 481 C90767 222 259 294 333 359 393 416 428 C90768 247 287 327 360 381 405 416 421 C90769 267 316 357 397 426 452 472 484 C90770 238 282 320 349 373 397 417 426 C90771 266 311 351 382 413 441 461 470 C90772 253 304 352 386 416 442 465 479 Covance 6329-212 3M T-6316.1 PAGE: 2 WEEK WEEK 910 488 509 536 559 490 505 439 457 560 573 472 487 503 514 434 452 472 491 474 492 578 600 526 539 569 595 557 567 504 520 489 499 497 511 485 502 456 472 551 569 470 490 566 591 499 504 450 467 445 459 439 439 486 504 497 514 1228 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 1 - 0 PPM C90773 259 311 361 394 425 453 476 496 C90774 257 301 343 380 404 430 450 462 C90775 262 305 344 381 C90776 287 338 379 420 454 477 509 526 C90777 280 326 370 412 452 483 515 537 C90778 287 326 361 397 420 444 460 466 C90779 242 295 341 375 410 431 452 461 C90780 252 300 344 392 431 461 487 503 GROUP: MALE 2 - 3 PPM C90781 281 320 359 394 423 436 456 472 C90782 255 303 343 374 407 429 449 457 C90783 223 263 301 333 363 391 413 418 C90784 253 298 340 373 400 422 441 447 C90785 254 302 345 379 404 432 456 469 C90786 265 311 355 386 413 437 456 464 C90787 249 300 339 377 411 442 467 486 C90788 260 311 356 396 422 451 470 487 C90789 279 333 385 440 C90790 279 328 374 417 439 476 498 513 C90791 221 263 303 335 360 388 408 419 C90792 249 295 339 380 409 443 463 482 C90793 246 295 337 373 402 429 449 464 C90794 245 297 347 385 421 452 475 493 C90795 271 318 361 392 418 441 451 469 C90796 284 332 367 398 418 433 457 472 C90797 249 295 333 373 405 435 457 476 C90798 270 320 370 410 402 434 457 474 C90799 242 288 336 368 396 419 433 449 C90800 286 332 377 414 439 474 499 518 Covance 6329-212 3M T-6316.1 PAGE: 3 WEEK WEEK 910 514 530 481 496 552 569 569 588 486 496 476 497 539 560 500 518 477 495 440 459 470 492 495 514 488 495 515 534 510 526 548 566 443 454 508 530 484 500 525 544 493 512 497 511 504 520 492 521 473 484 543 562 1229 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 2 - 3 PPM C90801 271 312 352 388 415 441 460 477 C90802 230 268 302 334 360 384 403 414 C90803 261 317 373 413 449 487 510 515 C90804 306 354 409 455 489 523 550 571 C90805 279 326 368 402 427 461 488 498 C90806 246 284 319 347 368 394 403 414 C90807 288 330 374 415 438 464 477 482 C90808 276 326 367 406 440 471 501 508 C90809 244 288 324 351 391 411 436 451 C90810 269 316 368 406 438 472 495 511 C90811 261 306 354 393 419 447 468 483 C90812 256 311 356 394 426 450 478 490 C90813 239 278 314 345 366 389 404 375 C90814 235 274 312 348 C90815 253 297 344 387 419 451 482 495 C90816 293 342 393 424 451 473 495 517 C90817 256 302 341 375 C90818 257 302 346 387 412 442 464 478 C90819 281 331 377 418 446 477 500 510 C90820 267 313 348 383 414 445 469 482 C90821 287 339 388 438 470 506 538 553 C90822 222 259 292 327 349 377 394 408 C90823 228 270 309 344 354 384 399 409 C90824 234 288 333 378 403 436 463 469 C90825 271 318 357 396 419 451 482 500 C90826 262 310 359 393 418 455 473 498 C90827 243 291 334 379 410 439 465 485 C90828 251 303 352 400 435 464 497 508 C90829 272 325 378 418 451 483 508 526 C90830 263 312 351 393 423 457 475 489 C90831 265 314 351 385 415 444 468 485 Covance 6329-212 3M T-6316.1 PAGE: 4 WEEK WEEK 910 503 518 431 448 546 550 597 619 523 539 421 440 514 530 539 556 473 483 539 557 517 529 515 535 524 532 535 556 499 515 537 551 508 523 579 597 428 445 436 451 496 511 525 543 530 540 508 528 534 556 554 581 512 530 519 540 1230 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 2 - 3 PPM C90832 255 311 361 404 443 467 494 507 C90833 271 315 358 399 C90834 273 324 378 426 C90835 276 318 355 388 406 434 451 464 C90836 247 294 330 363 396 417 436 450 C90837 235 274 308 344 371 392 414 425 C90838 300 359 416 461 490 523 549 564 C90839 294 347 395 440 481 513 547 557 C90840 245 293 341 385 418 455 476 495 GROUP: MALE 3 - 30 PPM C90841 230 277 317 345 372 404 426 444 C90842 220 254 278 305 328 348 364 380 C90843 245 294 338 376 409 442 471 480 C90844 271 314 350 393 401 427 440 446 C90845 286 330 358 398 415 446 465 474 C90846 251 294 326 358 C90847 251 290 333 371 397 419 439 448 C90848 246 288 329 369 402 432 451 467 C90849 264 312 359 398 425 456 486 494 C90850 254 289 327 358 383 405 423 439 C90851 249 293 338 381 406 433 451 457 C90852 264 307 347 381 410 431 457 463 C90853 238 273 304 337 C90854 267 310 352 384 412 437 459 480 C90855 267 315 360 400 422 465 486 495 C90856 261 314 356 392 421 448 469 486 C90857 273 317 360 394 421 447 471 484 C90858 228 263 298 324 350 371 389 405 C90859 269 310 360 403 432 459 480 494 Covance 6329-212 3M T-6316.1 PAGE: 5 WEEK WEEK 910 532 546 484 496 475 488 448 463 587 606 593 605 523 548 471 482 395 408 459 524 468 482 500 513 467 486 495 514 522 541 456 468 476 486 488 498 501 516 528 540 503 516 498 519 424 430 507 518 1231 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 3 - 30 PPM C90860 234 275 312 340 361 381 394 396 C90861 269 302 339 371 400 432 453 461 C90862 206 234 267 291 308 336 350 359 C90863 286 350 409 465 500 537 562 582 C90864 263 305 344 375 394 415 433 444 C90865 252 294 325 362 382 402 421 430 C90866 295 332 364 390 C90867 255 296 335 363 388 407 425 445 C90868 292 333 377 427 456 485 511 520 C90869 263 315 366 410 438 467 486 498 C90870 274 317 359 387 422 449 475 489 C90871 256 303 345 377 405 430 446 455 C90872 276 315 357 390 413 438 459 466 C90873 299 342 393 424 460 488 515 527 C90874 280 333 383 426 456 490 520 540 C90875 238 285 328 362 387 415 430 446 C90876 294 350 407 460 488 512 528 545 C90877 255 294 333 372 396 426 435 444 C90878 223 260 299 330 351 374 391 405 C90879 257 308 348 385 408 440 456 471 C90880 281 335 388 429 454 485 505 521 C90881 264 308 361 401 433 460 479 496 C90882 265 313 358 389 413 442 464 479 C90883 263 315 357 397 431 451 471 484 C90884 248 289 329 370 391 418 438 447 C90885 255 298 343 375 403 424 439 452 C90886 271 321 362 400 428 464 484 495 C90887 274 314 358 391 418 442 460 472 C90888 282 320 357 397 415 432 444 457 C90889 249 299 343 378 396 417 435 454 C90890 283 321 358 398 Covance 6329-212 3M T-6316.1 PAGE: 6 WEEK 9 WEEK 10 414 420 483 493 374 381 607 632 467 472 451 458 457 464 546 563 515 535 516 531 472 482 487 498 546 558 571 585 463 487 568 594 457 450 432 440 491 508 549 566 515 535 498 511 505 515 463 473 465 474 520 543 493 506 476 486 469 481 1232 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 3 - 30 PPM C90891 268 315 375 414 C90892 214 234 252 277 299 323 343 359 C90893 243 286 324 355 379 409 431 440 C90894 267 310 342 363 390 419 432 451 C90895 245 283 320 344 356 381 404 420 C90896 276 314 356 387 409 428 445 458 C90897 286 327 376 418 454 489 508 529 C90898 265 302 337 374 398 426 445 459 C90899 231 264 303 334 352 376 389 404 C90900 256 290 334 374 403 431 444 459 GROUP: MALE 4 - 100 PPM C90901 243 278 312 340 365 389 402 414 C90902 243 284 319 346 374 396 410 421 C90903 237 273 306 332 359 379 398 409 C90904 263 304 343 368 391 410 422 439 C90905 241 276 307 337 361 378 393 406 C90906 261 292 325 358 388 411 426 443 C90907 266 299 328 361 382 410 426 437 C90908 274 315 351 383 415 436 460 472 C90909 275 325 374 411 445 468 489 513 C90910 239 276 308 341 367 392 420 434 C90911 259 297 334 361 391 415 438 453 C90912 282 323 361 396 429 459 479 497 C90913 239 283 329 366 396 419 430 393 C90914 275 326 366 404 432 462 483 504 C90915 269 308 348 388 425 451 481 494 C90916 231 268 304 331 356 374 389 404 C90917 258 306 346 385 422 450 467 483 C90918 299 341 387 415 440 468 488 508 Covance 6329-212 3M T-6316.1 PAGE: 7 WEEK 9 WEEK 10 372 385 458 471 460 475 443 456 458 477 547 567 477 484 423 429 474 487 423 431 435 444 422 431 451 457 419 428 461 484 449 456 492 495 539 546 449 458 468 487 509 527 420 456 521 533 512 522 422 430 500 503 525 528 1233 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 4 - 100 PPM C90919 261 306 348 381 416 444 468 486 C90920 256 293 329 360 390 412 427 443 C90921 263 301 337 366 395 418 443 463 C90922 265 294 334 364 388 410 423 432 C90923 298 323 356 385 409 426 445 456 C90924 250 283 321 359 383 414 437 457 C90925 275 307 334 358 374 398 414 419 C90926 274 314 357 393 418 451 472 487 C90927 251 296 338 375 405 433 450 458 C90928 252 289 325 355 382 407 423 437 C90929 228 266 300 324 345 370 386 393 C90930 270 313 349 375 400 409 429 448 C90931 274 307 340 367 398 430 452 462 C90932 246 273 297 323 341 354 373 381 C90933 270 320 368 397 430 457 477 490 C90934 241 280 316 342 364 391 405 417 C90935 284 324 370 398 C90936 235 278 319 349 368 390 410 427 C90937 254 291 333 367 391 399 424 439 C90938 222 257 296 323 349 365 381 401 C90939 247 282 316 342 C90940 287 340 379 415 454 477 497 508 C90941 264 306 347 382 412 448 468 489 C90942 249 285 325 355 C90943 260 301 341 370 393 413 427 440 C90944 224 255 288 316 337 356 373 388 C90945 254 287 324 348 370 392 403 422 C90946 267 305 340 370 400 432 453 459 C90947 273 304 335 363 389 406 428 437 C90948 263 303 340 368 389 411 426 434 C90949 252 283 307 330 347 352 366 360 Covance 6329-212 3M T-6316.1 PAGE: 8 WEEK 9 WEEK 10 501 515 464 479 481 496 453 462 470 480 478 490 430 437 510 529 479 494 449 462 407 422 465 472 477 497 403 416 512 524 429 442 441 453 444 453 421 434 530 547 510 516 441 461 410 423 439 446 471 483 442 453 452 465 355 344 1234 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 4 - 100 PPM C90950 263 313 356 387 410 436 456 471 C90951 279 315 357 382 390 421 441 456 C90952 278 335 384 433 465 492 519 532 C90953 247 293 332 368 398 421 437 457 C90954 223 267 304 335 357 382 398 413 C90955 274 319 357 386 403 431 444 459 C90956 258 302 346 383 405 441 463 485 C90957 238 276 319 342 367 395 416 384 C90958 269 314 354 385 407 436 455 465 C90959 247 291 330 368 388 415 437 454 C90960 255 297 335 364 390 422 439 457 C90961 228 269 301 334 C90962 273 323 365 397 423 452 475 493 C90963 279 323 367 407 435 467 481 502 C90964 298 348 402 441 474 502 524 539 C90965 228 266 304 314 348 367 382 387 C90966 295 339 387 418 C90967 243 281 320 352 380 411 433 447 C90968 232 271 309 340 366 393 414 433 C90969 274 318 366 399 425 450 473 493 C90970 240 282 316 344 367 386 403 409 GROUP: MALE 5 - 300 PPM C90971 218 233 242 256 268 277 290 291 C90972 260 274 295 312 319 325 327 326 C90973 268 309 344 365 379 399 412 418 C90974 250 286 317 339 356 368 384 395 C90975 267 299 331 363 376 393 406 420 C90976 235 269 293 313 327 340 352 354 C90977 259 287 314 327 340 347 349 354 Covance 6329-212 3M T-6316.1 PAGE: 9 WEEK 9 WEEK 10 494 503 474 479 549 560 472 481 428 438 466 476 500 506 423 441 475 485 479 486 471 485 509 512 516 532 561 572 402 410 465 475 448 461 511 531 424 433 1235 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 5 - 3 0 0 PPM C90978 263 293 325 344 364 373 381 387 C90979 244 271 301 322 333 351 359 372 C90980 253 296 330 357 371 396 403 407 C90981 257 292 326 349 360 377 391 398 C90982 252 287 326 355 377 391 414 419 C90983 209 227 248 257 263 273 285 281 C90984 281 309 339 361 C90985 247 276 300 313 322 339 340 346 C90986 276 305 339 362 381 395 402 402 C90987 266 297 324 341 363 376 386 399 C90988 257 285 309 319 328 332 340 335 C90989 268 312 349 373 399 420 430 442 C90990 271 297 325 346 362 378 397 409 C90991 237 272 315 337 362 377 390 401 C90992 258 292 325 349 365 383 399 409 C90993 277 304 336 362 384 408 415 407 C90994 284 317 346 371 386 410 424 424 C90995 268 298 326 348 367 382 392 403 C90996 284 322 354 377 392 405 412 420 C90997 269 296 324 334 345 359 368 374 C90998 244 271 299 322 337 360 380 392 C90999 273 303 328 345 363 378 388 392 C91000 242 277 297 310 323 333 333 332 C91001 275 317 350 377 407 436 453 465 C91002 241 269 294 314 326 333 339 344 C91003 246 282 311 325 341 354 365 C91004 258 279 307 326 339 348 355 357 C91005 244 274 306 322 C91006 245 265 288 304 310 318 317 311 C91007 252 286 315 336 357 369 383 395 C91008 224 253 283 299 309 320 328 331 Covance 6329-212 3M T-6316.1 PAGE: 10 WEEK WEEK 910 1236 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 5 - 3 0 0 PPM C91009 285 312 337 349 355 364 374 378 C91010 257 278 304 321 EXCLUDE 342 342 350 C91011 254 294 322 349 370 397 412 430 C91012 239 266 292 314 327 342 344 346 C91013 304 343 377 395 407 423 435 449 C91014 224 253 279 299 317 335 344 355 C91015 270 303 330 350 361 378 390 406 C91016 252 278 306 321 334 348 356 366 C91017 273 299 324 335 347 362 363 364 C91018 285 327 365 393 411 430 443 463 C91019 258 287 322 345 359 368 383 386 C91020 238 267 290 313 324 335 343 348 C91021 251 287 323 350 376 393 401 307 C91022 273 307 345 367 386 408 418 433 C91023 253 285 318 345 368 389 404 416 C91024 210 237 270 285 C91025 356 390 419 438 454 465 478 C91026 268 292 319 336 359 377 386 397 C91027 227 256 290 310 330 339 350 359 C91028 262 299 337 356 380 406 419 430 C91029 245 280 306 322 334 346 352 C91030 247 280 311 331 355 374 393 404 C91031 238 262 291 301 318 326 328 323 C91032 240 281 324 356 378 407 418 419 C91033 260 291 321 341 C91034 233 267 302 329 351 371 380 390 C91035 273 304 329 348 354 380 397 408 C91036 261 289 306 326 338 351 357 358 C91037 282 315 348 366 C91038 276 301 334 348 374 385 395 405 C91039 277 320 356 384 407 429 440 437 Covance 6329-212 3M T-6316.1 PAGE: 11 WEEK WEEK 910 1237 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 5 - 300 PPM C91040 271 297 323 345 361 380 388 395 GROUP: MALE 6 - 100 PPM C91041 266 312 354 386 425 452 476 502 C91042 248 292 326 351 371 395 401 416 C91043 257 291 322 344 370 396 412 430 C91044 259 298 331 358 395 414 430 447 C91045 241 298 343 384 423 448 476 497 C91046 249 297 340 374 406 440 377 C91047 240 274 306 334 361 383 394 416 C91048 259 300 339 369 388 414 429 443 C91049 256 305 358 393 429 456 477 503 C91050 264 307 349 383 419 435 461 470 C91051 261 303 343 377 403 439 451 477 C91052 270 322 370 407 447 478 501 522 C91053 269 307 341 365 385 407 415 431 C91054 247 297 348 382 409 442 455 477 C91055 264 310 355 390 422 454 477 499 C91056 254 292 333 359 387 412 422 441 C91057 280 320 360 389 414 440 453 475 C91058 267 310 353 379 407 433 443 459 C91059 254 294 336 370 397 429 445 461 C91060 238 268 298 324 346 372 379 398 C91061 271 309 345 380 408 436 448 473 C91062 242 289 329 365 389 415 433 459 C91063 215 249 282 308 326 348 362 373 C91064 248 284 314 345 377 414 426 447 C91065 237 278 313 342 371 396 410 427 C91066 270 314 349 386 413 440 453 473 C91067 257 303 339 369 397 420 437 461 Covance 6329-212 3M T-6316.1 PAGE: 12 WEEK 9 WEEK 10 520 542 428 440 449 462 459 468 513 528 380 413 460 471 520 536 504 497 500 512 546 557 453 457 505 507 521 537 400 437 500 509 470 476 481 493 412 420 494 505 478 487 387 389 470 478 443 450 492 500 481 491 1238 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 6 - 100 PPM C91068 217 244 262 272 279 281 273 276 C91069 239 285 320 349 374 396 417 434 C91070 261 301 331 359 386 406 416 433 C91071 279 329 370 410 444 474 494 520 C91072 224 264 301 334 355 378 389 410 C91073 290 333 380 415 444 470 485 502 C91074 234 277 312 340 365 383 393 415 C91075 246 288 324 354 378 405 414 435 C91076 276 312 351 373 392 413 367 409 C91077 231 274 311 346 372 399 411 431 C91078 245 287 322 353 379 400 411 431 C91079 287 328 360 389 414 439 455 476 C91080 266 307 339 364 388 407 416 436 GROUP: MALE 7 - 300 PPM C91081 252 282 301 329 350 363 382 391 C91082 240 267 293 312 321 337 338 350 C91083 241 269 286 304 320 331 340 352 C91084 250 272 301 325 342 351 362 374 C91085 236 272 296 312 321 334 331 331 C91086 262 292 316 340 353 372 381 397 C91087 252 283 314 335 346 356 353 362 C91088 251 280 305 328 345 352 355 361 C91089 266 301 333 360 386 406 421 440 C91090 269 294 329 354 372 388 399 418 C91091 251 283 313 329 353 369 372 383 C91092 266 305 335 361 386 400 413 425 C91093 262 291 321 340 336 371 384 402 C91094 243 274 298 315 329 332 343 355 C91095 275 303 330 347 362 369 385 385 Covance 6329-212 3M T-6316.1 PAGE: 13 WEEK 9 WEEK 10 276 221 465 476 450 463 540 551 421 427 521 537 427 437 454 459 439 453 451 463 445 448 488 495 453 465 1239 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 7 - 300 PPM C91096 243 268 288 311 328 341 348 353 C91097 261 302 328 346 362 383 385 401 C91098 283 316 337 355 376 392 394 406 C91099 240 267 274 280 290 292 C91100 259 285 307 317 340 350 349 361 C91101 285 319 349 374 394 411 424 432 C91102 252 282 306 323 340 351 361 369 C91103 233 263 287 318 324 332 347 352 C91104 202 221 249 266 283 295 300 314 C91105 272 291 316 326 343 352 356 367 C91106 263 292 318 344 355 367 383 396 C91107 222 250 280 304 323 338 344 355 C91108 247 276 302 327 347 366 377 395 C91109 238 268 296 310 330 343 351 364 C91110 257 286 311 338 356 375 381 391 C91111 237 269 298 312 331 352 365 378 C91112 272 304 344 371 394 408 420 439 C91113 242 269 290 306 313 322 311 327 C91114 295 331 360 393 411 429 437 447 C91115 280 318 353 378 400 414 424 440 C91116 273 295 323 341 352 369 375 397 C91117 284 314 346 369 388 406 421 431 C91118 234 275 305 333 352 372 386 399 C91119 274 311 349 380 407 430 443 460 C91120 271 301 329 357 384 404 354 291 GROUP: FEMALE 1 - 0 PPM C91121 157 178 195 222 243 257 259 271 C91122 185 216 238 263 282 299 298 309 C91123 150 167 195 206 228 241 245 258 Covance 6329-212 3M T-6316.1 PAGE: 14 WEEK 9 WEEK 10 287 290 318 317 254 262 1240 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 1 - 0 PPM C91124 164 192 216 232 251 268 275 278 C91125 147 165 182 192 199 210 228 226 C91126 155 181 204 224 234 250 258 257 C91127 166 191 203 229 241 255 259 268 C91128 182 204 242 258 277 307 308 323 C91129 189 210 243 274 296 306 320 330 C91130 170 203 217 230 239 253 271 269 C91131 154 169 181 199 213 222 228 234 C91132 143 160 177 197 212 226 240 243 C91133 177 194 216 241 246 266 278 291 C91134 170 201 220 241 252 266 272 281 C91135 156 179 198 212 C91136 175 206 228 251 267 274 296 302 C91137 159 167 186 200 203 211 223 229 C91138 148 172 186 191 191 202 213 218 C91139 154 187 200 222 233 254 255 275 C91140 182 209 241 263 283 307 325 326 C91141 167 186 196 222 234 246 252 266 C91142 183 213 239 268 284 307 319 328 C91143 139 157 171 198 C91144 169 186 197 215 225 231 235 246 C91145 194 216 239 248 259 274 285 286 C91146 157 184 214 227 240 261 276 282 C91147 177 202 225 232 252 257 265 270 C91148 162 180 195 218 229 237 241 254 C91149 138 149 164 184 192 199 208 218 C91150 169 189 203 226 241 247 252 260 C91151 172 201 221 237 262 295 304 325 C91152 172 195 219 238 242 260 265 276 C91153 171 205 237 247 279 297 306 312 C91154 180 209 237 251 269 285 285 291 Covance 6329-212 3M T-6316.1 PAGE: 15 WEEK 9 WEEK 10 290 288 234 244 280 286 341 343 349 357 274 282 246 250 259 263 297 298 290 294 306 320 237 237 217 226 283 289 277 282 343 347 253 257 285 298 276 286 263 269 230 236 269 279 323 340 289 293 315 331 309 322 1241 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 1 - 0 PPM C91155 172 188 216 233 248 256 272 272 C91156 170 194 222 237 251 258 277 279 C91157 164 179 189 210 220 228 228 234 C91158 156 182 203 222 232 249 262 266 C91159 165 171 201 205 215 218 234 235 C91160 160 177 198 215 222 238 243 249 C91161 152 176 199 213 240 255 268 283 C91162 148 158 188 205 219 233 246 251 C91163 170 202 226 250 262 270 291 286 C91164 186 208 235 249 263 286 280 297 C91165 175 202 228 244 C91166 176 206 225 251 262 277 291 293 C91167 148 175 191 211 228 239 242 251 C91168 149 172 194 216 231 243 248 259 C91169 162 181 210 219 C91170 173 209 241 263 279 298 322 327 C91171 149 166 194 211 C91172 159 188 211 230 254 267 277 278 C91173 159 186 211 224 235 242 252 257 C91174 163 178 198 204 217 224 233 242 C91175 191 218 250 270 292 296 323 329 C91176 169 200 238 256 280 289 308 319 C91177 169 183 202 216 219 228 231 243 C91178 176 198 224 235 239 261 269 266 C91179 185 214 227 256 268 285 289 297 C91180 158 181 211 227 240 254 265 270 C91181 171 206 243 265 273 295 309 310 C91182 170 192 227 252 257 278 291 295 C91183 171 197 215 238 263 274 285 293 C91184 148 171 202 222 250 269 279 282 C91185 173 198 217 237 252 263 282 291 Covance 6329-212 3M T-6316.1 PAGE: 16 WEEK 9 WEEK 10 283 294 272 278 249 253 271 287 236 248 258 259 279 299 307 316 300 310 270 273 270 272 343 352 289 296 261 271 252 255 343 355 331 353 252 254 278 288 307 311 286 277 314 321 309 320 306 313 295 307 300 317 1242 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 1 - 0 PPM C91186 173 194 214 239 250 261 265 277 C91187 165 197 223 241 257 277 290 294 C91188 164 173 176 196 211 220 232 239 C91189 133 150 164 180 187 199 207 210 C91190 173 192 197 227 234 240 244 245 GROUP: FEMALE 2 - 3 PPM C91191 181 206 228 244 259 276 285 294 C91192 135 158 185 199 217 223 238 239 C91193 153 168 176 197 211 222 227 236 C91194 162 181 206 230 241 247 268 273 C91195 182 205 234 259 274 278 293 305 C91196 155 172 191 214 219 225 233 238 C91197 164 185 208 227 245 249 271 282 C91198 181 207 227 251 C91199 145 168 191 214 224 238 247 259 C91200 184 195 197 219 226 232 239 234 C91201 153 178 199 222 239 249 261 265 C91202 187 209 250 265 282 292 298 299 C91203 170 189 209 231 246 269 272 276 C91204 164 177 198 215 223 239 250 250 C91205 137 156 171 189 C91206 164 184 208 232 248 264 271 277 C91207 158 187 213 230 249 261 272 273 C91208 156 185 212 229 239 256 271 268 C91209 167 193 204 226 235 248 266 262 C91210 157 182 202 220 223 249 255 249 C91211 169 202 227 238 266 266 281 286 C91212 159 181 199 222 233 243 251 258 C91213 161 186 201 226 238 247 250 263 Covance 6329-212 3M T-6316.1 PAGE: 17 WEEK 9 WEEK 10 296 300 304 319 243 251 217 227 253 260 304 329 246 258 249 259 283 290 312 309 244 249 284 293 273 277 245 243 269 270 312 319 289 288 264 266 294 300 280 292 275 285 276 277 259 263 296 305 264 261 273 276 1243 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 2 - 3 PPM C91214 167 193 212 241 238 259 284 273 C91215 152 174 188 197 207 215 226 227 C91216 170 192 208 228 238 248 254 263 C91217 154 171 186 207 228 235 239 252 C91218 166 181 196 202 221 228 238 235 C91219 171 183 193 210 218 218 230 240 C91220 155 180 206 229 244 257 273 269 C91221 152 183 215 232 248 255 264 277 C91222 185 213 240 265 C91223 144 155 169 184 194 204 209 213 C91224 139 157 172 189 203 210 221 224 C91225 148 170 195 229 221 238 259 269 C91226 165 183 199 218 223 241 257 266 C91227 185 202 211 230 248 253 258 270 C91228 174 200 224 238 247 263 275 277 C91229 166 189 202 220 229 239 247 243 C91230 156 187 211 229 C91231 169 196 224 245 263 274 290 295 C91232 154 176 187 197 212 216 226 224 C91233 160 183 196 200 217 229 240 241 C91234 198 224 264 290 309 325 336 339 C91235 163 171 194 209 212 217 230 230 C91236 153 175 184 211 221 228 229 236 C91237 153 175 205 219 C91238 151 170 187 200 213 221 225 227 C91239 167 191 204 232 246 257 269 274 C91240 186 209 230 249 266 274 282 285 C91241 165 187 210 223 240 256 264 272 C91242 169 191 211 225 241 247 249 253 C91243 168 191 211 238 243 257 263 273 C91244 145 169 188 204 219 223 232 239 Covance 6329-212 3M T-6316.1 PAGE: 18 WEEK 9 WEEK 10 291 296 239 236 272 280 266 269 249 262 242 244 284 288 287 289 217 223 235 239 259 274 284 286 276 283 284 298 258 261 305 311 235 238 250 262 353 367 236 241 243 240 239 247 284 290 301 305 286 288 262 270 282 292 252 251 1244 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 2 - 3 PPM C91245 173 186 200 217 231 227 246 254 C91246 172 191 204 213 227 237 248 237 C91247 174 200 223 237 244 255 271 281 C91248 174 203 226 242 261 267 271 285 C91249 164 189 216 238 246 264 278 291 C91250 146 168 186 200 202 212 220 223 GROUP: FEMALE 3 - 30 PPM C91251 176 194 212 235 C91252 161 189 216 236 248 262 267 276 C91253 170 180 205 209 227 239 246 242 C91254 157 170 183 201 215 225 230 237 C91255 168 171 187 194 202 206 218 224 C91256 164 178 200 221 232 244 253 257 C91257 171 188 198 221 225 234 247 244 C91258 163 185 213 235 237 247 261 264 C91259 159 183 199 216 236 242 254 259 C91260 189 215 228 242 252 268 279 279 C91261 155 170 184 192 213 223 232 238 C91262 163 179 188 195 205 211 216 215 C91263 162 189 201 224 235 247 257 261 C91264 148 171 197 207 225 235 248 251 C91265 180 195 213 227 242 250 261 263 C91266 169 191 225 244 259 273 297 291 C91267 135 150 168 182 190 203 210 213 C91268 156 172 191 205 217 221 230 239 C91269 172 197 226 242 253 267 271 270 C91270 140 160 173 192 203 217 224 233 C91271 183 199 223 240 246 265 280 282 C91272 181 200 227 237 Covance 6329-212 3M T-6316.1 PAGE: 19 WEEK 9 WEEK 10 268 279 245 256 298 305 293 293 305 302 245 235 296 304 259 256 246 246 229 244 256 262 252 258 270 284 269 274 285 294 265 265 223 228 271 277 265 263 268 274 305 310 222 220 242 245 290 296 247 252 290 286 1245 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 3 - 30 PPM C91273 146 166 185 206 211 234 237 244 C91274 165 174 201 206 219 228 237 240 C91275 174 186 219 234 246 261 276 283 C91276 151 166 180 202 209 218 224 233 C91277 147 166 194 211 216 226 239 245 C91278 176 194 217 233 241 255 263 267 C91279 188 209 227 256 281 288 301 315 C91280 157 183 210 227 233 250 254 263 C91281 163 183 212 224 237 246 258 260 C91282 166 194 211 244 255 272 288 301 C91283 152 176 200 221 231 239 259 267 C91284 162 186 213 230 238 255 263 259 C91285 181 200 218 229 C91286 158 167 176 177 195 198 199 206 C91287 152 167 178 195 C91288 190 213 232 248 253 265 269 266 C91289 149 171 187 210 224 233 238 245 C91290 175 202 217 236 255 253 260 266 C91291 167 191 209 228 C91292 168 191 217 229 249 266 268 265 C91293 154 177 196 220 232 247 258 270 C91294 161 181 208 222 236 251 251 260 C91295 152 179 193 223 222 238 233 242 C91296 159 173 190 193 209 223 228 219 C91297 168 185 194 226 241 243 240 258 C91298 179 205 223 236 244 259 270 267 C91299 147 165 179 192 195 211 217 219 C91300 153 180 198 219 224 240 254 260 C91301 146 171 182 207 213 228 238 241 C91302 191 208 219 238 250 262 264 272 C91303 171 196 219 241 259 266 280 292 Covance 6329-212 3M T-6316.1 PAGE: 20 WEEK 9 WEEK 10 255 261 245 252 289 301 242 247 254 259 274 275 329 334 267 277 275 275 300 320 272 274 268 280 218 220 269 273 253 254 282 282 285 291 277 282 267 272 245 258 236 235 267 274 272 277 224 227 271 268 257 269 274 275 304 308 1246 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 3 - 30 PPM C91304 177 209 229 247 263 285 285 294 C91305 177 200 226 239 258 277 285 290 C91306 170 191 215 229 236 250 255 254 C91307 183 206 220 227 254 267 269 271 C91308 167 187 213 231 250 253 268 276 C91309 164 192 213 243 251 273 283 301 C91310 157 180 202 216 219 241 250 253 GROUP: FEMALE 4 - 100 PPM C91311 158 160 168 182 186 198 209 217 C91312 160 169 184 193 201 209 215 216 C91313 155 177 202 220 223 231 241 250 C91314 155 175 190 201 209 216 226 228 C91315 153 164 176 189 194 200 206 214 C91316 157 170 193 211 C91317 164 189 212 233 C91318 169 188 208 220 227 237 240 243 C91319 140 155 180 195 217 227 236 243 C91320 182 195 203 210 222 232 236 233 C91321 164 173 203 219 235 249 250 262 C91322 171 178 201 219 228 226 241 251 C91323 167 187 198 212 219 229 235 247 C91324 153 176 196 204 222 231 235 232 C91325 175 190 206 217 227 237 241 252 C91326 141 152 161 177 187 195 195 207 C91327 158 171 190 203 216 225 227 231 C91328 165 184 203 212 222 232 237 246 C91329 146 170 186 203 216 222 233 234 C91330 156 177 192 209 219 218 230 232 C91331 159 176 196 219 221 241 246 262 Covance 6329-212 3M T-6316.1 PAGE: 21 WEEK 9 WEEK 10 311 309 302 310 260 266 290 295 280 289 303 312 253 262 218 226 223 231 254 259 229 232 222 229 246 248 244 253 236 238 255 267 257 261 245 248 243 249 253 262 217 216 241 243 251 259 246 249 240 244 260 268 1247 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 4 - 100 PPM C91332 157 174 193 212 217 228 230 233 C91333 160 174 191 204 C91334 150 175 201 216 231 242 251 261 C91335 154 171 188 204 214 219 228 235 C91336 187 200 214 221 230 234 233 236 C91337 181 205 223 241 253 264 273 270 C91338 150 161 174 192 202 210 214 217 C91339 162 177 196 214 224 232 243 241 C91340 170 196 229 250 266 280 287 295 C91341 175 191 208 228 242 253 260 270 C91342 176 192 212 223 228 239 246 247 C91343 167 179 200 207 220 227 239 244 C91344 188 202 217 228 241 247 242 255 C91345 165 173 193 206 C91346 166 180 210 230 242 258 260 275 C91347 173 194 212 224 234 241 244 246 C91348 159 178 197 212 225 223 230 235 C91349 168 189 211 216 219 236 240 246 C91350 152 168 183 197 C91351 186 198 225 236 252 260 280 283 C91352 183 207 227 249 260 269 280 288 C91353 167 179 200 212 222 232 228 233 C91354 164 180 196 207 220 231 227 233 C91355 146 166 187 199 216 228 233 240 C91356 174 191 213 233 254 262 258 268 C91357 152 161 172 188 197 205 202 210 C91358 185 196 224 239 256 264 259 266 C91359 160 176 194 212 220 227 222 234 C91360 154 170 185 195 203 213 214 223 C91361 178 193 217 234 243 254 258 268 C91362 193 213 227 237 242 254 251 256 Covance 6329-212 3M T-6316.1 PAGE: 22 WEEK 9 WEEK 10 229 229 261 261 239 244 240 236 277 286 215 215 253 255 296 298 273 281 251 255 246 248 260 259 279 281 253 263 233 233 246 262 292 299 291 290 237 240 241 241 249 258 278 274 214 224 272 268 237 244 228 227 264 278 258 262 1248 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 4 - 100 PPM C91363 169 190 216 241 253 256 258 270 C91364 149 163 177 194 206 221 222 228 C91365 174 180 188 192 198 201 198 205 C91366 164 192 197 218 235 237 247 261 C91367 186 213 233 255 261 272 275 279 C91368 165 184 202 215 220 232 235 245 C91369 163 178 193 206 213 228 230 239 C91370 153 166 179 194 196 205 213 216 C91371 170 187 211 235 250 266 262 281 C91372 162 180 202 223 235 247 247 255 C91373 163 180 193 199 211 222 226 227 C91374 174 197 219 239 249 258 259 267 C91375 157 179 201 212 225 236 237 244 C91376 158 176 192 209 218 229 234 244 C91377 151 173 192 209 220 229 225 232 C91378 168 185 201 215 226 234 236 245 C91379 147 164 186 204 215 226 230 236 C91380 180 205 225 245 255 266 264 278 GROUP: FEMALE 5 - 300 PPM C91381 159 166 179 190 195 204 210 215 C91382 152 156 159 170 171 177 171 182 C91383 155 163 174 187 191 203 202 204 C91384 167 171 184 189 188 200 204 207 C91385 158 167 180 193 194 204 203 209 C91386 158 162 172 179 196 200 202 216 C91387 166 169 171 178 185 188 190 193 C91388 177 185 195 205 212 219 217 226 C91389 152 154 166 163 176 180 185 193 C91390 147 159 165 178 190 194 195 206 Covance 6329-212 3M T-6316.1 PAGE: 23 WEEK 9 WEEK 10 278 274 235 237 208 221 271 272 290 286 245 246 245 258 220 227 281 290 262 259 237 245 268 273 247 257 251 245 241 250 249 247 237 241 283 284 1249 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 5 - 3 0 0 PPM C91391 164 176 189 195 205 213 210 219 C91392 159 176 184 189 191 206 211 217 C91393 150 152 144 160 154 160 161 173 C91394 181 185 191 198 198 200 205 214 C91395 143 152 165 172 178 189 190 189 C91396 184 198 212 216 225 224 231 241 C91397 146 152 159 174 179 190 195 209 C91398 158 174 182 197 209 212 213 219 C91399 175 185 194 204 208 212 211 224 C91400 183 196 205 218 226 231 232 233 C91401 142 158 170 173 174 178 178 182 C91402 148 155 161 167 174 177 176 183 C91403 169 187 196 206 210 217 212 218 C91404 151 160 173 182 191 196 204 207 C91405 158 166 176 186 195 204 208 217 C91406 170 172 179 190 190 198 203 208 C91407 157 163 172 183 189 200 202 210 C91408 173 191 205 216 225 233 235 249 C91409 174 175 176 184 179 197 193 193 C91410 167 162 178 184 189 184 189 200 C91411 160 177 197 210 213 221 228 234 C91412 164 176 183 193 188 202 211 207 C91413 159 176 194 198 207 212 216 219 C91414 168 177 189 202 209 224 230 234 C91415 183 177 181 184 182 196 195 194 C91416 190 201 215 224 229 234 239 248 C91417 161 177 189 195 199 198 204 207 C91418 177 178 189 193 199 205 203 213 C91419 169 182 189 197 209 220 227 236 C91420 155 169 185 195 201 207 210 224 C91421 140 151 163 172 175 191 195 203 Covance 6329-212 3M T-6316.1 PAGE: 24 WEEK WEEK 910 1250 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 5 - 3 0 0 PPM C91422 177 179 186 197 198 199 202 211 C91423 170 179 198 207 C91424 173 169 178 193 196 204 210 213 C91425 174 180 184 195 190 195 204 214 C91426 146 152 150 157 157 163 171 177 C91427 172 192 207 216 C91428 160 175 184 198 207 219 218 224 C91429 194 209 222 226 234 242 241 251 C91430 149 156 167 180 C91431 155 170 178 182 185 192 197 209 C91432 187 190 204 204 215 220 217 222 C91433 178 188 193 201 199 201 200 212 C91434 159 170 179 184 192 188 192 198 C91435 171 180 191 205 212 222 223 232 C91436 159 154 170 176 182 184 191 203 C91437 161 178 191 205 216 224 229 243 C91438 143 159 167 179 187 195 193 204 C91439 162 181 191 199 209 216 210 213 C91440 171 186 198 204 209 215 214 231 C91441 156 164 175 181 194 198 193 213 C91442 164 174 180 193 194 202 205 210 C91443 184 183 195 209 C91444 152 166 172 185 194 201 201 207 C91445 166 166 174 179 185 186 194 203 C91446 141 144 153 158 164 176 184 192 C91447 162 173 181 191 192 202 203 217 C91448 158 167 178 187 C91449 170 183 193 199 206 210 214 223 C91450 175 177 183 191 201 190 193 197 Covance 6329-212 3M T-6316.1 PAGE: 25 WEEK WEEK 910 1251 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 6 - 100 PPM C91451 158 167 189 202 208 224 225 230 C91452 168 184 206 219 234 247 249 258 C91453 154 168 193 207 218 226 239 248 C91454 161 178 198 205 213 221 222 235 C91455 158 173 191 206 215 219 226 224 C91456 160 172 187 200 206 210 220 225 C91457 152 178 202 219 227 240 254 257 C91458 154 174 192 204 212 224 225 222 C91459 189 208 232 243 252 262 267 266 C91460 142 157 178 194 196 196 200 197 C91461 169 187 209 223 234 244 253 257 C91462 156 171 197 221 229 239 247 252 C91463 189 200 225 229 237 248 249 256 C91464 147 160 181 194 204 214 225 225 C91465 149 165 182 189 201 212 220 215 C91466 179 205 228 253 268 270 264 279 C91467 162 177 199 207 218 224 237 244 C91468 167 178 187 196 196 211 215 203 C91469 163 178 198 214 218 227 234 244 C91470 184 200 230 248 251 266 280 279 C91471 143 161 184 196 206 220 223 226 C91472 179 196 220 224 230 244 249 256 C91473 160 176 191 204 208 220 227 229 C91474 176 198 216 234 247 256 268 269 C91475 162 182 208 217 234 248 251 264 C91476 173 193 206 216 229 239 249 257 C91477 155 176 190 201 213 223 227 236 C91478 184 207 230 235 241 250 258 259 C91479 166 190 215 224 233 249 254 260 C91480 159 176 196 210 214 225 230 241 C91481 147 158 177 194 205 217 226 226 Covance 6329-212 3M T-6316.1 PAGE: 26 WEEK 9 WEEK 10 238 235 272 275 256 254 236 237 235 242 229 230 258 268 237 243 267 275 200 195 265 265 263 271 258 260 226 225 225 228 279 297 245 247 219 209 247 246 293 288 238 240 256 265 227 235 283 287 268 277 258 263 242 243 261 268 264 271 243 244 232 235 1252 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 6 - 100 PPM C91482 153 167 189 204 217 228 232 239 C91483 170 196 216 227 237 248 252 252 C91484 185 195 212 229 228 245 247 243 C91485 159 172 189 206 216 221 228 233 C91486 173 190 212 218 234 242 247 249 C91487 158 179 194 209 220 228 235 238 C91488 202 227 241 252 264 271 274 C91489 167 176 194 203 217 225 234 227 C91490 157 180 194 209 220 227 231 234 GROUP: FEMALE 7 - 300 PPM C91491 156 165 184 195 201 203 207 211 C91492 155 165 179 191 194 205 207 209 C91493 159 163 175 180 188 198 203 205 C91494 157 168 176 185 189 197 201 202 C91495 163 174 186 194 194 200 201 210 C91496 147 164 180 184 186 195 197 205 C91497 168 180 192 205 211 219 225 226 C91498 154 157 174 183 186 185 201 200 C91499 155 166 174 184 189 188 192 193 C91500 179 188 200 208 202 208 208 210 C91501 183 185 182 198 196 203 210 209 C91502 173 186 192 208 211 221 222 229 C91503 189 199 215 221 229 243 241 246 C91504 162 167 171 186 186 193 202 204 C91505 161 169 180 192 193 204 213 213 C91506 172 181 193 201 210 221 222 226 C91507 148 164 176 180 193 197 195 200 C91508 172 184 200 209 212 219 219 223 C91509 165 179 196 202 208 213 212 212 Covance 6329-212 3M T-6316.1 PAGE: 27 WEEK 9 WEEK 10 250 252 257 267 256 257 244 244 261 263 249 253 276 285 238 251 241 245 1253 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 7 - 3 0 0 PPM C91510 166 182 200 210 219 226 230 232 C91511 164 181 184 194 203 209 207 213 C91512 170 176 191 199 199 204 214 213 C91513 165 177 191 201 206 210 205 213 C91514 167 187 197 205 209 221 222 225 C91515 156 174 181 190 194 202 209 214 C91516 159 171 182 192 197 202 207 207 C91517 141 158 169 175 174 183 191 197 C91518 158 175 192 201 214 217 224 233 C91519 172 183 183 185 183 187 185 178 C91520 152 165 177 184 195 203 211 213 C91521 163 175 185 200 197 210 210 209 C91522 160 179 187 196 199 201 199 201 C91523 142 160 170 179 189 193 202 208 C91524 181 189 203 216 219 224 225 226 C91525 164 180 189 196 199 204 209 212 C91526 178 194 201 208 209 214 218 226 C91527 186 191 205 213 221 225 227 241 C91528 134 142 151 162 166 167 174 173 C91529 167 190 200 216 222 226 225 232 C91530 164 176 189 196 203 207 217 219 Covance 6329-212 3M T-6316.1 PAGE: 28 WEEK WEEK 910 1254 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 1 - 0 PPM C90711 505 516 524 526 529 540 545 570 C90712 503 515 522 501 513 528 529 559 C90713 563 578 582 590 596 608 611 638 C90714 509 521 531 544 546 552 552 576 C90715 539 546 550 565 575 592 603 619 C90716 492 503 508 519 511 525 543 567 C90720 591 600 614 624 633 645 660 686 C90721 625 646 666 694 715 725 764 813 C90722 477 486 492 502 501 514 524 552 C90723 577 558 550 582 593 607 618 642 C90724 498 511 522 535 541 550 563 586 C90726 509 518 527 537 543 553 568 596 C90727 621 636 635 650 655 666 675 699 C90728 523 531 555 560 569 567 584 625 C90729 543 553 557 564 579 590 597 616 C90730 534 546 555 577 588 601 609 641 C90731 588 597 616 634 C90733 517 529 543 557 562 569 580 611 C90734 635 656 671 682 692 698 715 751 C90735 479 491 498 505 507 512 524 544 C90736 524 538 542 558 566 577 587 617 C90738 485 497 507 519 519 526 540 570 C90739 506 521 539 547 556 566 577 608 C90740 526 544 551 561 567 580 597 639 C90741 487 503 515 529 534 542 555 582 C90742 516 522 526 536 547 555 571 591 C90743 577 588 605 611 623 634 646 696 C90744 524 534 547 556 565 571 587 609 C90745 474 472 487 497 498 505 519 553 C90746 589 597 553 585 C90747 498 503 516 523 536 546 552 583 C90748 528 533 541 547 Covance 6329-212 3M T-6316.1 PAGE: 29 WEEK 25 586 586 658 595 653 594 723 852 571 668 616 621 727 655 639 682 626 781 564 652 594 636 665 615 610 724 640 575 596 WEEK 29 606 601 673 601 676 611 745 890 601 691 642 631 738 686 651 714 641 814 574 667 607 645 684 621 629 746 667 589 598 1255 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 1 - 0 PPM C90749 465 473 481 484 497 507 514 541 C90750 500 507 510 522 527 532 544 569 C90751 499 513 520 530 540 542 558 579 C90752 610 625 640 650 656 667 681 696 C90754 551 563 574 579 591 596 604 638 C90755 610 634 643 657 667 686 695 746 C90757 584 599 608 617 613 632 642 671 C90758 534 551 561 564 570 575 586 620 C90759 511 522 539 540 540 554 562 577 C90760 521 538 561 573 573 583 601 639 C90761 520 538 547 555 556 567 576 607 C90762 479 491 506 512 522 528 539 562 C90763 575 585 602 600 611 621 626 659 C90764 503 518 527 536 548 551 557 578 C90765 602 619 630 640 646 656 666 693 C90766 516 526 533 547 545 556 562 607 C90767 481 496 510 515 524 539 546 581 C90768 468 480 496 499 C90770 452 469 475 484 494 501 513 541 C90771 513 513 520 525 534 538 553 574 C90772 529 542 560 565 559 574 588 625 C90773 548 555 577 585 590 600 609 649 C90774 511 519 531 538 544 549 562 582 C90776 582 595 610 624 635 647 663 690 C90777 605 613 632 640 653 661 674 706 C90778 503 512 516 520 520 526 528 543 C90779 516 522 541 544 560 570 590 613 C90780 572 585 604 617 Covance 6329-212 3M T-6316.1 PAGE: 30 WEEK 25 WEEK 29 559 567 591 606 604 618 719 720 663 689 789 803 696 715 643 678 595 609 672 700 631 648 585 599 676 684 589 612 712 739 637 640 617 648 569 587 588 598 650 678 679 711 596 609 730 754 729 757 558 567 648 675 1256 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 2 - 3 PPM C90781 525 548 563 577 588 606 605 635 C90782 508 522 533 532 544 557 567 592 C90783 463 475 477 485 489 500 506 520 C90784 495 509 512 526 531 536 545 565 C90785 533 542 561 567 574 590 600 624 C90786 505 518 527 528 537 545 547 564 C90787 547 566 581 583 594 609 615 645 C90788 540 553 566 572 581 590 608 637 C90790 577 592 605 609 615 625 632 673 C90791 461 473 479 481 479 495 501 526 C90792 540 554 563 568 573 571 547 600 C90793 504 517 533 537 543 555 563 587 C90794 557 562 577 590 600 617 627 661 C90795 522 535 541 550 558 562 577 608 C90796 522 532 462 510 529 546 557 585 C90797 527 546 557 569 C90798 526 541 546 548 553 568 578 605 C90799 482 499 507 514 518 524 531 561 C90800 576 585 600 609 617 621 631 654 C90801 527 544 550 565 574 581 584 612 C90802 458 465 473 479 481 483 498 526 C90803 569 581 592 597 607 623 635 664 C90804 629 641 645 659 656 675 675 696 C90805 548 558 562 577 592 600 615 653 C90806 447 453 459 464 465 465 476 489 C90807 547 553 566 568 C90808 574 580 592 608 618 628 640 677 C90809 505 507 525 531 538 542 553 583 C90810 564 584 587 598 610 621 632 657 C90811 553 558 558 568 580 592 601 621 C90812 540 548 564 568 578 582 605 626 Covance 6329-212 3M T-6316.1 PAGE: 31 WEEK 25 WEEK 29 657 673 620 657 534 546 573 580 655 670 598 596 669 675 660 694 714 722 552 557 599 636 608 624 695 681 634 627 610 637 627 661 579 574 674 693 643 640 555 560 694 714 706 730 681 680 503 496 712 731 590 601 694 719 644 662 640 659 1257 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 2 - 3 PPM C90815 551 559 570 560 576 589 592 622 C90816 570 574 594 602 607 621 633 665 C90818 529 541 552 558 C90819 565 578 589 586 589 607 621 649 C90820 547 555 570 580 575 589 603 633 C90821 608 625 638 644 651 659 676 709 C90822 455 468 471 472 484 497 511 540 C90823 460 474 490 503 516 536 547 571 C90824 523 532 543 543 558 567 579 606 C90825 563 576 591 597 602 623 634 663 C90826 561 578 594 601 607 617 632 666 C90827 538 563 574 585 581 594 606 655 C90828 583 594 611 621 624 635 649 687 C90829 592 615 625 631 649 657 681 715 C90830 544 557 566 568 576 585 596 622 C90831 555 575 595 610 C90832 557 572 579 587 590 605 610 645 C90835 510 525 526 535 537 549 556 585 C90836 505 525 538 552 C90837 477 488 496 504 511 516 525 552 C90838 626 647 659 670 680 695 712 757 C90839 620 640 657 662 680 688 705 744 C90840 567 583 599 609 616 631 643 689 GROUP: MALE 3 - 30 PPM C90841 495 509 522 532 542 546 557 571 C90842 416 426 429 434 441 441 449 469 C90843 542 552 557 560 C90844 492 502 518 532 541 549 555 594 C90845 527 540 551 555 563 571 577 611 Covance 6329-212 3M T-6316.1 PAGE: 32 WEEK 25 WEEK 29 636 647 691 702 676 693 655 687 736 757 561 579 599 640 625 648 700 709 700 731 682 714 728 748 758 789 650 666 670 690 618 633 573 590 800 843 785 814 733 743 599 620 483 490 637 653 631 654 1258 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 3 - 30 PPM C90847 495 511 518 525 528 534 546 560 C90848 527 540 555 557 572 586 606 639 C90849 562 578 592 607 614 617 628 654 C90850 477 486 494 497 507 509 521 533 C90851 493 498 505 511 512 518 529 548 C90852 515 529 541 551 C90854 525 536 545 549 564 568 574 607 C90855 559 569 582 596 603 613 623 643 C90856 528 547 553 558 555 562 567 598 C90857 528 540 549 555 557 567 576 597 C90858 443 453 463 462 474 474 490 516 C90859 529 546 559 571 577 580 586 616 C90860 425 437 446 448 447 455 460 473 C90861 500 522 533 539 544 552 563 587 C90862 398 400 409 417 421 427 440 473 C90863 645 657 667 685 C90864 484 498 504 514 518 528 535 556 C90865 471 476 482 489 495 494 506 522 C90867 478 486 493 501 502 504 512 532 C90868 569 589 603 612 624 631 646 667 C90869 540 555 561 574 575 574 580 611 C90870 557 559 568 576 583 592 583 633 C90871 493 505 514 518 526 542 542 560 C90872 508 519 530 536 545 551 561 586 C90873 569 574 574 579 589 603 607 629 C90874 606 617 640 649 649 660 668 693 C90875 496 507 521 538 544 557 575 597 C90876 610 632 641 653 651 657 664 697 C90877 473 483 492 495 C90878 452 460 468 484 489 490 503 520 C90879 522 527 536 545 555 554 570 607 Covance 6329-212 3M T-6316.1 PAGE: 33 WEEK 25 WEEK 29 587 583 670 702 677 693 555 574 560 578 623 648 654 652 624 635 617 626 537 545 638 630 485 479 604 619 487 499 572 577 543 548 537 547 694 721 624 652 663 674 567 565 604 625 644 665 716 745 623 639 741 754 529 538 637 669 1259 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 3 - 30 PPM C90880 582 593 596 611 C90881 548 562 575 587 593 601 616 642 C90882 524 534 539 548 557 564 576 599 C90883 527 539 549 530 559 566 572 590 C90884 483 490 495 497 502 506 514 536 C90885 491 511 519 523 537 542 556 599 C90886 553 567 578 587 594 603 616 654 C90887 528 539 548 561 570 586 603 636 C90888 502 512 517 521 501 517 538 557 C90889 491 501 503 513 511 515 523 553 C90892 402 410 420 424 420 430 440 462 C90893 477 496 503 510 518 523 530 561 C90894 486 503 507 518 534 538 557 589 C90895 482 494 512 514 522 533 545 565 C90896 484 502 510 517 526 534 538 554 C90897 573 593 607 619 599 613 633 673 C90898 505 513 521 526 533 542 549 572 C90899 440 446 452 466 466 474 478 494 C90900 507 519 527 534 540 550 562 575 GROUP: MALE 4 - 100 PPM C90901 439 449 458 461 463 474 475 501 C90902 452 463 475 484 488 503 510 534 C90903 431 442 452 457 459 469 482 498 C90904 464 476 478 488 492 497 498 512 C90905 444 449 455 468 C90906 488 503 519 522 533 539 546 576 C90907 470 480 483 492 C90908 507 515 532 539 545 553 559 587 C90909 560 572 590 604 609 617 631 657 Covance 6329-212 3M T-6316.1 PAGE: 34 WEEK 25 WEEK 29 670 695 616 631 604 624 537 551 634 653 677 681 669 704 573 575 570 580 479 501 576 604 608 634 584 613 572 581 710 732 597 608 515 528 591 610 519 539 551 577 516 538 530 535 597 631 599 637 695 713 1260 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 4 - 100 PPM C90910 474 485 490 500 504 516 525 536 C90911 496 508 513 521 520 534 536 554 C90912 531 541 555 557 560 568 573 589 C90913 473 488 495 497 496 506 505 512 C90914 543 555 566 582 589 597 606 626 C90915 534 548 558 562 570 583 588 606 C90916 433 451 457 465 469 480 485 507 C90917 507 523 531 528 C90918 527 541 546 559 562 573 574 597 C90919 529 539 545 545 551 554 557 582 C90920 491 499 510 520 526 531 544 569 C90921 518 532 547 553 C90922 478 488 498 509 512 524 527 557 C90923 492 504 511 521 530 534 538 559 C90924 512 534 543 544 552 557 568 613 C90925 445 455 463 471 476 487 499 518 C90926 536 551 557 565 573 586 597 631 C90927 498 507 517 522 530 537 547 565 C90928 466 472 475 479 485 493 509 514 C90929 429 438 442 413 442 459 471 496 C90930 481 494 504 510 519 527 534 542 C90931 503 515 526 532 539 546 551 579 C90932 427 438 447 456 460 470 471 496 C90933 534 542 551 558 565 576 589 619 C90934 448 457 462 466 465 483 488 500 C90936 464 477 486 493 503 516 524 528 C90937 465 474 474 474 476 475 472 500 C90938 444 453 457 465 464 473 483 507 C90940 556 575 578 592 600 603 614 644 C90941 523 536 536 550 552 562 571 585 C90943 465 480 488 488 495 497 494 459 Covance 6329-212 3M T-6316.1 PAGE: 35 WEEK 25 WEEK 29 559 568 568 582 594 627 539 557 646 667 637 663 530 537 605 629 599 620 580 602 572 583 564 585 605 597 533 550 646 678 591 607 531 549 524 533 551 571 587 605 513 523 630 650 512 528 556 577 502 520 526 553 659 673 616 638 515 537 1261 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 4 - 100 PPM C90944 430 433 440 448 446 456 463 482 C90945 447 454 468 473 480 489 492 499 C90946 495 500 507 519 518 530 530 555 C90947 461 476 485 493 492 499 518 537 C90948 467 475 484 487 491 501 512 523 C90949 339 305 266 C90950 515 527 535 550 556 559 573 592 C90951 489 506 509 515 527 528 541 558 C90952 572 573 585 596 598 604 622 645 C90953 490 497 511 523 516 525 536 542 C90954 446 461 462 468 471 476 489 507 C90955 491 502 505 517 521 533 536 557 C90956 481 504 514 518 525 535 542 575 C90957 448 471 470 479 480 492 508 490 C90958 490 498 493 506 515 531 545 570 C90959 498 496 504 509 522 528 541 569 C90960 495 500 507 516 C90962 531 546 550 554 558 570 586 604 C90963 540 548 557 563 574 579 587 605 C90964 591 599 611 613 614 629 636 666 C90965 418 422 431 392 414 420 431 442 C90967 491 505 516 522 528 535 541 561 C90968 472 483 503 509 509 520 526 552 C90969 554 569 584 598 605 617 618 641 C90970 445 458 466 474 486 487 495 517 GROUP: MALE 6 - 100 PPM C91041 559 568 582 588 597 599 618 629 C91042 450 466 484 494 497 501 516 539 C91043 469 484 496 506 509 513 519 531 Covance 6329-212 3M T-6316.1 PAGE: 36 WEEK 25 WEEK 29 491 498 521 524 568 588 551 572 549 569 598 618 564 588 676 706 547 562 526 541 566 577 594 608 510 526 586 600 590 607 629 639 613 621 690 715 458 462 574 589 565 589 655 681 538 553 642 648 549 585 552 575 1262 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 6 - 100 PPM C91044 481 491 504 509 511 521 526 543 C91045 538 552 570 580 585 581 601 628 C91047 425 438 457 461 462 469 480 482 C91048 487 497 509 522 526 530 539 561 C91049 548 566 572 578 585 608 619 655 C91050 513 528 544 558 565 565 558 586 C91051 529 543 555 564 571 578 583 616 C91052 577 591 617 616 628 639 653 698 C91053 464 474 485 495 500 505 515 538 C91054 513 513 529 534 536 544 546 583 C91055 551 569 584 592 600 616 624 660 C91056 460 478 500 505 519 526 541 561 C91057 513 526 541 538 550 560 574 582 C91058 479 490 496 496 507 519 521 535 C91059 503 516 520 532 537 542 545 559 C91060 436 449 452 454 459 470 477 495 C91061 521 526 543 555 569 572 585 599 C91062 491 506 514 522 532 542 554 562 C91063 393 401 410 414 418 425 427 439 C91064 490 503 515 527 537 547 562 581 C91065 457 468 482 488 489 502 512 511 C91066 513 524 535 543 550 557 562 587 C91067 500 514 521 531 536 545 557 581 C91068 198 195 C91069 488 501 520 527 535 544 557 602 C91070 476 481 489 494 500 510 517 532 C91071 562 578 586 596 603 614 624 638 C91072 436 445 447 452 454 461 474 478 C91073 548 563 585 595 608 622 643 660 C91074 444 452 457 467 473 474 479 506 C91075 472 477 489 497 497 511 524 550 Covance 6329-212 3M T-6316.1 PAGE: 37 WEEK 25 WEEK 29 561 583 645 664 478 496 597 614 694 725 596 614 630 650 736 779 563 570 604 628 674 702 585 608 601 622 549 560 571 599 507 525 606 612 577 598 442 456 598 618 532 543 597 614 596 618 623 659 546 554 646 662 492 505 680 703 508 526 572 601 1263 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 6 - 100 PPM C91076 465 487 500 510 511 525 530 560 C91077 474 483 493 499 499 512 527 544 C91078 458 470 477 482 489 491 503 525 C91079 504 517 522 523 527 532 540 557 C91080 480 496 501 508 508 513 523 544 GROUP: FEMALE 1 - 0 PPM C91121 286 302 310 310 312 321 332 344 C91122 331 346 334 328 329 335 337 349 C91123 262 272 273 278 275 277 281 300 C91124 291 298 304 298 294 303 310 311 C91125 250 253 251 257 265 263 264 280 C91127 290 290 299 295 298 304 310 319 C91128 343 362 362 362 373 372 387 402 C91129 367 368 387 402 C91130 288 282 283 290 291 296 290 301 C91131 248 258 262 265 267 269 275 286 C91132 273 275 284 291 290 295 303 320 C91133 299 315 321 321 319 330 332 346 C91134 302 308 301 315 325 322 335 355 C91136 322 329 328 338 340 358 349 373 C91137 247 249 247 246 254 262 259 259 C91138 232 238 240 252 257 258 257 263 C91139 298 297 310 306 316 315 332 331 C91141 278 295 298 298 293 304 315 323 C91142 361 368 361 377 380 383 387 408 C91144 252 260 265 262 257 263 265 270 C91146 308 309 313 321 324 329 330 346 C91147 291 295 307 313 318 325 332 348 C91148 274 284 289 286 280 288 295 313 Covance 6329-212 3M T-6316.1 PAGE: 38 WEEK 25 WEEK 29 581 608 519 543 542 545 575 596 561 574 340 361 374 394 303 319 321 321 297 313 332 339 435 445 302 317 288 290 345 361 365 358 380 395 388 405 268 275 268 283 346 363 342 348 428 430 277 281 359 370 371 391 311 322 1264 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 1 - 0 PPM C91149 234 239 250 253 258 265 266 277 C91150 279 292 294 298 299 305 312 319 C91151 335 360 363 370 371 382 386 408 C91152 289 296 300 294 302 304 312 322 C91153 326 343 349 347 355 354 365 371 C91154 308 322 333 324 335 334 344 355 C91155 294 297 300 304 C91156 275 292 302 303 309 324 331 356 C91157 249 258 259 255 253 260 265 282 C91158 294 296 293 306 314 319 319 332 C91159 254 257 258 268 277 275 274 291 C91160 270 264 276 280 C91161 303 312 307 317 317 320 334 340 C91164 325 323 340 335 339 344 349 361 C91166 319 325 324 326 327 329 333 344 C91167 273 287 294 291 290 290 300 328 C91168 272 280 286 286 286 293 297 310 C91170 368 369 364 365 369 384 384 416 C91172 295 293 301 307 303 304 307 315 C91173 275 282 288 291 296 297 300 307 C91174 264 275 283 284 C91175 374 385 387 385 399 406 414 432 C91176 356 374 370 364 364 371 375 406 C91177 261 265 267 275 287 298 299 291 C91178 292 287 300 300 293 307 313 337 C91179 316 326 328 328 326 336 332 344 C91180 295 295 300 307 317 328 337 360 C91181 326 327 330 332 C91182 331 329 347 349 346 349 360 378 C91183 315 325 324 326 327 328 340 347 C91184 314 321 330 328 333 343 347 364 Covance 6329-212 3M T-6316.1 PAGE: 39 WEEK 25 WEEK 29 297 304 336 346 429 450 331 333 426 456 362 387 378 408 293 291 342 366 297 309 362 369 379 438 353 363 352 349 314 323 439 455 308 323 310 329 444 458 413 431 307 333 327 344 360 383 392 416 398 427 360 356 383 399 1265 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 1 - 0 PPM C91185 327 332 344 341 346 362 357 382 C91186 300 309 315 311 312 317 325 336 C91187 333 333 342 345 349 346 347 361 C91188 260 262 269 266 275 284 283 290 C91189 234 235 243 244 247 250 252 257 C91190 268 270 269 270 271 278 282 293 GROUP: FEMALE 2 - 3 PPM C91191 326 328 329 326 336 337 331 371 C91192 269 271 268 276 C91193 259 261 274 275 271 276 279 295 C91194 302 310 307 309 313 320 323 328 C91195 321 325 323 321 326 334 323 342 C91196 249 255 268 268 265 273 280 293 C91197 308 301 304 307 307 312 319 334 C91199 288 290 291 295 300 308 307 318 C91200 262 265 264 268 270 272 272 290 C91201 276 278 286 293 292 294 302 319 C91202 318 326 332 331 327 341 349 357 C91203 292 301 304 313 321 330 340 352 C91204 277 280 290 295 281 299 305 327 C91206 309 318 320 324 325 338 337 364 C91207 296 297 295 301 304 310 308 320 C91208 277 285 288 288 291 292 299 314 C91209 288 283 281 291 298 291 301 304 C91210 266 271 276 276 277 280 287 300 C91211 318 308 317 311 321 323 328 347 C91212 269 273 280 286 292 292 295 308 C91213 276 287 290 288 302 307 309 318 C91214 302 307 301 307 314 327 331 330 Covance 6329-212 3M T-6316.1 PAGE: 40 WEEK 25 WEEK 29 412 423 346 347 375 391 306 307 261 271 308 311 394 418 304 311 360 377 372 380 317 336 354 370 321 333 294 303 322 335 381 403 379 397 337 349 404 421 325 342 324 332 319 330 319 337 359 378 323 335 339 356 366 371 1266 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 2 - 3 PPM C91215 237 242 247 250 251 252 262 267 C91216 278 283 288 289 290 300 306 309 C91217 270 275 285 282 283 289 295 304 C91218 252 254 265 267 270 273 286 294 C91219 248 252 258 252 263 275 275 286 C91220 294 303 308 304 310 319 322 325 C91221 290 298 307 315 308 314 320 341 C91223 229 232 237 241 C91224 241 238 251 251 257 260 268 272 C91225 273 272 271 281 282 284 297 307 C91226 297 299 306 318 320 327 334 356 C91227 285 293 299 298 301 312 314 317 C91228 295 298 298 310 302 305 318 315 C91229 258 266 274 289 281 289 286 304 C91231 321 330 332 333 337 347 353 368 C91232 240 242 250 254 254 255 266 278 C91233 260 263 274 276 C91234 367 360 370 380 385 391 405 425 C91235 242 247 250 249 250 259 262 270 C91236 239 245 254 247 245 246 248 262 C91238 248 253 259 260 261 260 267 268 C91239 297 298 305 304 309 311 313 333 C91240 305 295 288 290 309 305 309 319 C91241 299 303 309 314 C91242 272 273 278 283 282 279 287 297 C91243 300 297 308 304 317 319 329 333 C91244 253 260 266 265 267 264 279 288 C91245 289 290 292 299 309 305 326 339 C91246 248 247 255 267 264 266 279 291 C91247 312 317 322 327 335 339 348 371 C91248 305 317 318 310 Covance 6329-212 3M T-6316.1 PAGE: 41 WEEK 25 WEEK 29 284 301 312 324 304 317 302 317 312 355 352 358 353 355 287 288 316 315 380 407 329 340 333 340 304 310 382 405 286 288 441 450 278 273 279 280 291 300 343 354 334 340 299 307 361 373 306 310 374 376 293 314 401 421 1267 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 2 - 3 PPM C91249 321 324 333 333 329 342 346 372 C91250 247 239 246 247 251 245 265 269 GROUP: FEMALE 3 - 30 PPM C91252 309 316 310 314 317 319 322 339 C91253 262 269 267 276 273 275 278 297 C91254 243 252 257 259 253 259 266 272 C91255 246 262 271 279 275 279 291 291 C91256 266 270 274 278 279 278 285 296 C91257 253 256 259 262 261 263 270 277 C91258 284 286 292 294 294 297 294 314 C91259 281 283 294 295 297 301 308 321 C91260 297 307 312 312 315 326 329 349 C91261 254 251 264 269 268 266 278 294 C91262 222 224 228 229 229 233 236 252 C91263 281 292 297 303 308 317 325 350 C91264 266 259 275 272 272 281 283 299 C91265 278 280 285 280 287 289 298 300 C91266 312 319 323 319 323 317 319 318 C91267 224 232 233 232 239 241 239 254 C91268 252 258 263 260 258 268 271 288 C91269 299 297 308 311 313 309 323 329 C91270 263 287 270 267 272 276 287 306 C91271 295 298 300 304 307 305 313 322 C91273 269 277 285 286 287 289 294 305 C91274 257 264 270 269 271 272 286 289 C91275 300 311 315 317 323 328 333 345 C91276 241 249 256 249 248 258 263 273 C91277 259 268 269 272 279 285 284 292 C91278 276 286 287 285 288 293 296 310 Covance 6329-212 3M T-6316.1 PAGE: 42 WEEK 25 WEEK 29 382 410 271 271 348 375 300 323 276 288 309 316 306 316 288 300 329 337 336 358 368 383 303 315 257 265 375 394 302 299 310 321 354 367 275 275 309 330 337 372 315 314 328 328 315 326 310 334 376 393 285 292 294 302 317 327 1268 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 3 - 30 PPM C91279 331 346 354 358 357 367 370 417 C91280 279 277 282 289 290 300 299 306 C91281 284 281 294 285 C91282 319 324 330 332 347 345 357 374 C91283 285 293 292 289 289 290 296 319 C91284 282 277 279 290 293 291 290 314 C91286 212 218 226 228 228 237 244 254 C91288 279 278 278 288 C91289 246 260 265 262 265 269 271 282 C91290 272 289 294 295 302 310 320 348 C91292 293 289 295 302 305 316 322 342 C91293 285 296 303 301 C91294 279 276 280 286 286 289 292 307 C91295 259 256 264 262 265 271 268 297 C91296 241 235 239 244 259 270 259 261 C91297 266 279 288 283 282 296 303 318 C91298 284 280 283 287 294 294 296 308 C91299 232 234 240 241 C91300 286 285 288 291 283 299 304 314 C91301 271 277 277 283 279 289 295 308 C91302 280 288 285 292 289 290 300 310 C91303 315 322 329 336 343 356 360 361 C91304 322 323 331 339 C91305 312 313 316 323 314 328 334 367 C91306 271 270 266 271 274 274 276 288 C91307 293 293 299 295 300 302 302 316 C91308 283 288 289 297 299 308 307 314 C91309 317 319 318 331 332 336 347 362 C91310 265 266 270 276 278 278 282 292 Covance 6329-212 3M T-6316.1 PAGE: 43 WEEK 25 WEEK 29 453 476 322 330 390 406 338 345 323 331 262 280 297 307 364 378 351 359 315 325 298 313 261 277 332 335 299 307 340 361 333 342 320 331 381 394 367 387 295 293 331 336 326 331 377 385 308 324 1269 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 4 - 100 PPM C91311 223 224 223 224 224 227 232 239 C91312 230 233 231 238 240 241 246 252 C91313 257 261 263 262 261 275 269 273 C91314 237 236 237 239 240 245 247 254 C91315 237 240 242 243 240 248 251 263 C91318 247 244 244 248 246 C91319 252 255 262 262 263 270 275 277 C91320 248 250 253 256 257 261 267 273 C91321 268 261 267 263 272 272 276 277 C91322 265 267 265 269 274 275 289 301 C91323 253 255 263 265 273 275 280 292 C91324 248 248 248 248 251 250 259 258 C91325 260 263 267 273 274 274 280 283 C91326 208 213 219 215 203 211 213 223 C91327 247 250 243 255 252 250 262 270 C91328 260 259 266 264 269 267 274 270 C91329 250 258 260 256 C91330 254 249 252 257 256 266 268 271 C91331 274 281 274 285 282 289 293 311 C91332 235 238 235 242 237 242 243 249 C91334 265 266 274 275 277 276 286 289 C91335 246 250 251 251 257 257 260 267 C91336 239 245 247 243 247 248 252 258 C91337 286 289 295 294 C91338 213 214 212 217 215 222 228 231 C91339 259 260 264 265 266 269 272 282 C91340 302 301 297 298 299 299 300 304 C91341 277 278 280 282 280 285 286 298 C91342 259 259 267 267 265 269 275 285 C91343 246 251 257 258 258 265 262 275 C91344 264 267 265 260 266 268 274 278 Covance 6329-212 3M T-6316.1 PAGE: 44 WEEK 25 WEEK 29 248 259 261 260 282 290 260 270 267 275 288 313 285 292 288 301 303 311 265 271 288 295 233 240 275 284 295 303 295 305 332 362 258 260 298 308 272 274 254 261 235 242 285 304 317 321 308 319 289 292 282 295 276 279 1270 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 4 - 100 PPM C91346 287 292 302 302 308 307 318 326 C91347 267 261 264 273 270 270 277 282 C91348 239 237 240 239 236 241 247 251 C91349 260 261 252 265 262 261 271 279 C91351 301 308 306 313 313 316 324 325 C91352 296 294 290 299 299 305 311 322 C91353 239 247 244 248 253 255 253 260 C91354 239 238 244 244 240 240 246 245 C91355 270 276 281 283 C91356 276 279 275 274 271 274 275 293 C91357 218 224 224 229 227 228 231 240 C91358 283 284 280 288 286 291 299 311 C91359 243 245 247 249 249 258 258 271 C91360 230 233 237 239 240 242 247 247 C91361 272 269 276 284 278 281 284 293 C91362 262 263 263 267 273 274 273 282 C91363 282 288 294 300 300 303 305 310 C91364 239 239 248 243 254 247 251 255 C91365 211 216 215 220 219 228 225 225 C91366 268 276 272 279 284 281 286 293 C91367 297 296 291 295 293 302 299 311 C91368 251 249 253 255 255 257 262 264 C91369 268 266 269 274 281 282 287 295 C91370 235 232 237 235 C91371 293 282 290 298 297 308 295 314 C91372 265 270 275 273 270 278 284 296 C91373 240 240 236 245 250 252 262 272 C91374 275 275 278 282 279 282 286 292 C91375 259 259 257 266 268 267 266 276 C91376 248 255 256 253 257 258 264 266 C91377 252 249 257 261 259 261 265 276 Covance 6329-212 3M T-6316.1 PAGE: 45 WEEK 25 WEEK 29 331 349 290 298 258 259 282 299 332 328 327 336 271 275 248 252 308 311 242 249 322 328 281 282 259 263 301 304 301 311 326 322 260 268 232 243 302 314 328 340 273 278 304 311 327 337 298 309 298 322 299 303 281 286 271 280 292 306 1271 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 4 - 100 PPM C91378 245 251 246 249 251 256 258 262 C91379 247 249 251 252 C91380 289 286 297 293 303 297 304 314 GROUP: FEMALE 6 - 100 PPM C91451 242 246 251 253 254 253 262 263 C91452 274 279 284 289 289 290 292 298 C91453 260 263 269 265 272 276 280 286 C91454 237 242 247 247 249 251 258 260 C91455 246 247 246 250 247 250 255 257 C91456 238 242 247 246 250 255 261 263 C91457 264 267 270 276 280 280 290 299 C91458 245 240 244 254 250 254 256 264 C91459 275 284 284 288 288 291 288 297 C91460 199 211 214 214 216 221 215 233 C91461 267 282 279 279 289 293 308 331 C91462 268 275 279 282 290 295 297 303 C91463 266 265 266 261 264 271 276 274 C91464 228 234 228 232 234 241 234 241 C91465 232 229 233 241 240 241 249 249 C91466 289 299 289 301 298 302 304 311 C91467 251 256 258 255 260 264 264 272 C91468 221 213 220 226 230 224 234 252 C91469 253 254 254 259 261 264 266 269 C91470 300 296 301 306 313 315 315 321 C91471 248 251 249 259 259 263 265 274 C91472 268 273 268 271 276 283 281 285 C91473 237 242 239 244 248 248 250 255 C91474 286 291 294 299 302 304 306 314 C91475 273 281 286 289 285 292 298 313 Covance 6329-212 3M T-6316.1 PAGE: 46 WEEK 25 WEEK 29 272 276 315 320 278 284 316 323 299 304 272 280 272 273 279 286 310 317 275 280 307 319 239 241 354 382 316 329 282 285 250 261 272 290 331 335 272 283 263 269 276 279 334 334 287 300 297 308 263 267 318 316 326 332 1272 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 6 - 100 PPM C91476 267 267 267 271 275 274 279 292 C91477 247 257 261 263 268 269 274 280 C91478 272 272 274 279 280 281 286 293 C91479 273 279 278 278 285 284 288 300 C91480 252 255 260 260 257 258 261 269 C91481 235 231 233 231 230 243 242 245 C91482 249 255 259 261 265 268 270 276 C91483 263 264 270 270 272 272 280 289 C91484 256 253 259 264 267 266 267 261 C91485 243 251 251 247 239 251 257 264 C91486 264 263 271 276 283 282 283 286 C91487 250 257 257 257 258 256 261 266 C91488 285 289 290 291 295 300 302 311 C91489 246 245 254 260 260 261 270 279 C91490 248 252 258 259 257 259 261 272 Covance 6329-212 3M T-6316.1 PAGE: 47 WEEK 25 WEEK 29 309 314 297 311 310 319 311 330 277 281 254 257 288 295 289 297 280 304 276 279 296 299 291 302 315 324 289 301 279 287 1273 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 1 - 0 PPM C90711 621 633 644 656 673 687 693 711 C90712 614 617 630 613 642 652 659 688 C90713 693 708 725 744 778 787 801 821 C90714 599 613 621 649 666 681 C90715 693 716 718 734 755 771 789 804 C90716 639 658 671 694 712 736 745 765 C90720 765 791 806 838 862 888 888 923 C90721 921 953 823 C90722 620 639 642 671 685 706 719 740 C90723 716 730 725 756 789 798 795 794 C90724 666 678 695 720 735 755 766 795 C90726 639 646 650 665 678 692 695 690 C90727 748 765 760 785 793 807 806 823 C90728 709 733 745 785 820 838 856 879 C90729 672 680 677 703 712 736 755 773 C90730 729 764 769 804 826 839 836 796 C90733 653 670 676 689 703 718 722 655 C90734 843 861 876 910 922 941 950 978 C90735 595 613 626 645 664 676 689 703 C90736 678 696 711 734 747 782 795 816 C90738 618 643 662 687 695 705 708 718 C90739 648 656 673 699 700 724 745 755 C90740 699 721 728 759 780 804 810 769 C90741 636 676 694 713 731 762 776 778 C90742 643 644 651 666 671 693 715 717 C90743 778 808 822 849 878 910 922 942 C90744 688 702 709 742 766 784 794 813 C90745 588 611 620 647 647 668 686 713 C90747 621 633 646 652 668 682 688 705 C90749 598 609 613 630 651 662 674 697 C90750 621 640 629 647 677 693 710 735 C90751 637 653 666 699 725 751 Covance 6329-212 3M T-6316.1 PAGE: 48 WEEK 65 720 671 825 821 772 935 750 827 711 837 908 796 643 700 919 709 805 729 767 614 800 744 962 822 714 705 711 735 WEEK 69 730 691 838 845 797 961 772 848 707 842 927 812 532 731 926 732 719 735 776 807 764 966 835 726 674 729 763 1274 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 1 - 0 PPM C90752 733 737 745 783 790 811 C90754 710 738 749 789 807 837 C90755 841 869 869 919 966 985 994 1013 C90757 732 746 767 807 822 854 884 911 C90758 680 704 707 738 756 786 786 800 C90759 607 628 627 644 659 679 674 681 C90760 722 741 749 775 806 823 843 857 C90761 637 664 656 683 702 721 721 739 C90762 602 613 591 606 638 657 654 677 C90763 687 688 699 709 724 743 771 783 C90764 625 570 604 632 655 674 679 694 C90765 748 768 776 803 812 847 860 877 C90766 677 694 701 726 751 766 703 688 C90767 658 637 660 681 719 745 C90770 600 603 607 640 642 662 674 679 C90771 604 618 633 654 665 678 677 693 C90772 697 706 713 746 771 789 777 717 C90773 737 756 771 808 842 875 901 951 C90774 616 623 627 644 657 673 669 685 C90776 780 794 808 833 844 881 917 946 C90777 766 797 814 845 834 877 890 895 C90778 577 593 596 619 641 649 651 666 C90779 686 701 712 747 773 782 784 803 GROUP: MALE 2 - 3 PPM C90781 683 703 706 725 749 763 774 782 C90782 665 674 684 708 716 716 732 748 C90783 563 567 579 593 605 618 629 630 C90784 594 599 612 634 637 644 658 666 C90785 685 700 717 736 755 770 781 804 Covance 6329-212 3M T-6316.1 PAGE: 49 WEEK 65 WEEK 69 1035 939 822 692 868 752 689 796 700 878 685 709 910 695 962 906 668 833 792 773 639 676 808 1044 808 821 704 891 770 703 807 718 886 695 715 573 983 915 675 833 800 776 648 692 813 1275 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 2 - 3 PPM C90786 605 620 626 647 666 673 678 694 C90787 675 691 707 719 749 783 803 830 C90788 707 735 755 784 803 832 848 876 C90790 734 755 761 800 826 832 860 870 C90791 566 586 597 616 631 652 661 680 C90792 650 666 672 696 693 700 503 C90793 641 656 664 695 717 735 726 751 C90794 531 583 684 743 799 829 858 883 C90795 664 684 698 726 729 687 514 C90796 647 665 672 697 705 711 669 C90798 671 691 705 739 753 784 790 808 C90799 586 594 593 612 633 651 658 671 C90800 704 712 726 746 760 780 794 813 C90801 669 686 699 739 672 746 760 779 C90802 587 605 609 631 654 667 675 689 C90803 735 760 775 797 819 837 841 851 C90804 744 754 767 800 804 832 853 856 C90805 704 723 729 744 777 800 634 480 C90806 617 C90808 756 761 767 796 818 839 863 880 C90809 609 623 634 638 654 648 667 675 C90810 736 762 781 834 862 884 899 937 C90811 666 683 698 704 639 724 747 752 C90812 675 690 708 727 737 757 747 752 C90815 641 648 643 674 682 704 723 732 C90816 741 773 779 809 837 859 878 909 C90819 721 749 779 819 851 861 889 920 C90820 690 701 720 752 775 788 810 828 C90821 780 805 810 848 877 903 919 941 C90822 606 630 645 645 657 681 687 706 C90823 642 666 679 704 722 741 757 757 Covance 6329-212 3M T-6316.1 PAGE: 50 WEEK 65 WEEK 69 701 702 851 873 899 914 906 879 690 703 758 768 897 901 818 843 686 691 828 830 797 812 673 700 823 796 866 863 717 517 688 696 941 965 773 795 775 778 745 749 925 942 931 963 845 729 950 961 710 715 760 763 1276 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 2 - 3 PPM C90824 659 684 694 724 755 778 785 821 C90825 723 723 723 743 767 790 809 838 C90826 731 742 748 772 798 807 832 847 C90827 736 757 764 794 834 854 901 923 C90828 768 774 791 824 868 883 859 814 C90829 818 851 871 923 950 981 991 1016 C90830 676 682 697 708 726 744 753 759 C90832 702 720 747 768 801 818 832 851 C90835 638 642 639 664 689 720 722 730 C90837 604 615 600 616 625 608 549 505 C90838 872 902 891 942 986 1014 1040 1072 C90839 825 860 884 916 943 971 983 997 C90840 783 816 829 866 887 908 950 986 GROUP: MALE 3 - 30 PPM C90841 649 677 682 700 713 739 756 772 C90842 510 512 511 527 538 561 574 591 C90844 684 698 716 738 768 797 776 815 C90845 667 680 687 710 736 756 781 805 C90847 611 622 623 662 683 701 720 742 C90848 744 752 790 825 859 899 918 961 C90849 729 736 738 780 820 832 859 872 C90850 591 602 605 620 641 650 673 694 C90851 594 598 608 632 652 668 695 711 C90854 662 667 681 697 728 744 744 772 C90855 677 671 678 695 707 728 744 761 C90856 663 679 695 721 739 757 764 782 C90857 649 664 664 673 691 704 715 720 C90858 565 584 589 619 646 666 681 689 C90859 639 653 655 684 719 752 736 671 Covance 6329-212 3M T-6316.1 PAGE: 51 WEEK 65 WEEK 69 834 835 850 950 774 1022 772 867 735 486 1094 1008 1010 791 590 841 821 751 969 863 716 731 771 775 801 725 705 552 851 856 854 755 1050 794 878 763 455 1111 1043 970 797 604 867 852 772 993 790 725 744 780 792 826 730 711 554 1277 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 3 - 30 PPM C90860 490 493 491 504 517 526 540 550 C90861 635 651 646 670 683 700 721 742 C90862 523 534 543 570 598 615 633 641 C90864 598 616 624 633 641 651 682 668 C90865 562 569 579 596 607 624 632 639 C90867 551 552 553 559 566 569 573 576 C90868 741 762 780 802 825 858 882 869 C90869 673 691 710 751 776 800 830 846 C90870 695 715 726 755 775 795 810 829 C90871 586 596 598 611 636 643 662 669 C90872 626 631 642 670 699 716 731 732 C90873 669 683 678 703 721 733 738 747 C90874 767 775 786 816 828 861 728 815 C90875 651 668 676 692 720 748 727 758 C90876 767 791 797 850 882 891 C90878 537 532 543 556 566 582 C90879 680 694 702 723 751 755 757 769 C90881 705 728 744 781 797 821 827 854 C90882 659 686 693 713 740 771 794 838 C90883 595 609 618 639 638 638 647 657 C90884 548 561 568 587 586 593 598 616 C90885 661 677 688 724 743 759 781 795 C90886 716 735 755 790 817 845 848 885 C90887 739 756 775 796 830 860 896 922 C90888 589 595 599 619 621 620 636 637 C90889 598 604 618 637 655 671 671 699 C90892 510 512 530 554 579 592 605 620 C90893 627 650 643 666 704 733 757 776 C90894 651 664 665 696 732 749 770 780 C90895 618 631 632 658 673 692 696 718 C90896 587 599 601 631 667 694 699 697 Covance 6329-212 3M T-6316.1 PAGE: 52 WEEK 65 WEEK 69 552 564 702 580 548 439 693 707 650 664 573 584 869 846 857 847 836 825 674 688 735 746 751 722 692 778 804 782 777 874 894 858 869 648 667 620 625 820 823 872 878 940 704 646 676 718 728 626 613 793 803 791 788 726 741 623 512 1278 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 3 - 30 PPM C90897 736 753 752 789 803 825 840 861 C90898 612 623 627 641 647 656 673 563 C90899 539 544 548 571 583 602 622 650 C90900 615 624 619 649 653 668 683 692 GROUP: MALE 4 - 100 PPM C90901 562 569 580 601 617 650 681 697 C90902 586 590 593 619 641 657 C90903 543 542 553 570 588 609 624 643 C90904 537 546 556 557 555 566 578 581 C90906 646 663 684 702 726 728 745 766 C90908 641 652 663 667 673 699 704 704 C90909 723 720 738 771 801 828 C90910 586 598 615 636 657 671 680 692 C90911 587 592 601 621 640 660 675 695 C90912 627 641 646 653 675 686 692 699 C90913 580 594 593 609 627 640 C90914 689 698 704 724 742 761 773 791 C90915 683 703 717 741 759 773 791 806 C90916 560 568 581 602 632 661 675 668 C90918 640 656 656 688 693 714 C90919 617 624 621 648 657 672 657 652 C90920 607 619 636 629 654 673 697 712 C90922 602 614 617 636 647 633 594 477 C90923 589 608 605 620 642 647 661 673 C90924 596 587 590 616 620 646 C90925 553 577 587 606 612 629 658 685 C90926 687 705 710 740 772 765 790 799 C90927 567 580 589 592 599 618 636 662 C90928 558 565 567 572 578 591 612 628 Covance 6329-212 3M T-6316.1 PAGE: 53 WEEK 65 WEEK 69 871 874 671 671 702 708 723 749 658 668 593 598 761 782 716 730 702 709 687 704 613 559 815 836 814 833 681 695 648 650 732 730 687 704 703 717 806 821 671 679 642 645 1279 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 4 - 100 PPM C90929 545 548 554 560 570 569 618 622 C90930 568 565 561 576 584 612 628 638 C90931 611 630 635 665 677 681 703 726 C90932 520 540 544 561 572 589 C90933 641 641 655 672 664 677 694 710 C90934 539 538 554 564 565 587 594 616 C90936 577 578 572 595 616 624 647 664 C90937 524 535 547 582 599 616 641 638 C90938 566 579 592 611 631 650 663 667 C90940 693 701 717 746 765 805 813 735 C90941 641 659 686 698 734 775 806 837 C90943 547 550 578 588 589 618 638 624 C90944 517 523 531 542 555 568 569 584 C90945 540 556 560 C90946 586 558 599 605 621 647 657 650 C90947 586 600 612 633 651 669 680 691 C90948 587 590 601 612 617 633 649 663 C90950 636 648 679 701 722 735 C90951 593 601 606 624 629 658 C90952 729 746 762 801 816 798 696 C90953 560 576 581 602 624 645 667 673 C90954 564 575 585 614 632 652 652 640 C90955 591 602 612 634 650 679 701 713 C90956 620 623 631 650 658 677 688 713 C90957 542 561 548 553 575 605 C90958 611 634 636 643 676 685 700 664 C90959 619 628 641 669 677 710 736 749 C90962 663 678 695 709 743 767 790 804 C90963 630 655 672 697 710 740 770 769 C90964 729 731 735 754 774 784 777 807 C90965 439 477 472 490 494 513 512 526 Covance 6329-212 3M T-6316.1 PAGE: 54 WEEK 65 WEEK 69 658 645 662 739 739 721 711 631 651 654 663 655 654 683 546 867 887 649 666 590 594 649 643 707 713 651 661 679 699 588 444 725 735 713 699 688 705 767 770 773 635 654 529 770 670 506 524 1280 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 4 - 100 PPM C90967 608 631 647 673 681 701 C90968 609 614 629 647 669 677 685 700 C90969 698 708 715 773 786 811 837 862 C90970 562 574 578 610 623 643 657 679 GROUP: MALE 6 - 100 PPM C91041 667 679 700 738 754 779 749 443 C91042 618 627 646 662 682 691 721 747 C91043 594 612 626 648 662 676 700 743 C91044 600 603 602 617 645 657 612 463 C91045 662 684 706 733 705 C91047 490 496 509 509 515 530 543 568 C91048 620 634 661 680 699 724 750 775 C91049 728 737 602 C91050 649 636 648 667 682 718 742 785 C91051 653 668 678 683 704 742 772 807 C91052 782 803 822 835 875 918 966 945 C91053 581 592 610 628 645 653 678 692 C91054 642 671 697 727 750 788 815 864 C91055 716 745 753 794 806 830 873 919 C91056 618 633 642 653 675 675 694 699 C91057 629 628 629 652 567 630 640 659 C91058 572 574 582 600 616 628 637 659 C91059 605 620 633 654 671 704 742 768 C91060 539 549 567 590 608 620 646 680 C91061 616 621 628 642 642 660 675 688 C91062 596 611 630 651 670 686 706 734 C91063 467 477 476 486 498 504 519 535 C91064 619 632 635 673 687 711 731 757 C91065 542 554 564 566 571 591 601 625 Covance 6329-212 3M T-6316.1 PAGE: 55 WEEK 65 WEEK 69 717 530 876 893 688 709 762 782 762 787 590 615 776 791 785 812 811 830 753 692 680 898 936 950 983 714 735 677 671 690 708 789 826 696 724 719 738 754 768 548 548 785 813 607 625 1281 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 6 - 100 PPM C91066 624 632 642 656 670 685 707 735 C91067 640 656 675 691 713 731 757 780 C91069 682 686 719 757 786 821 850 895 C91070 558 564 559 569 585 596 607 629 C91071 667 675 687 712 736 751 769 810 C91072 517 527 544 563 584 597 619 639 C91073 695 714 727 756 784 823 862 910 C91074 537 532 548 564 588 601 621 648 C91075 627 642 666 689 731 757 777 805 C91076 615 617 632 632 643 670 682 719 C91077 557 580 604 635 653 688 712 729 C91078 556 568 561 527 570 610 616 656 C91079 613 612 615 628 645 671 693 730 C91080 571 582 594 600 612 636 669 698 GROUP: FEMALE 1 - 0 PPM C91121 379 374 385 403 409 435 C91122 388 395 396 435 455 479 C91123 326 341 356 372 392 398 413 422 C91124 335 333 342 359 376 389 C91125 312 328 340 357 376 392 399 421 C91127 355 358 379 400 427 453 458 486 C91128 470 467 499 525 547 565 581 590 C91130 326 335 355 395 381 410 425 435 C91131 299 299 301 309 316 310 318 329 C91132 391 404 421 447 466 480 492 520 C91133 372 401 388 403 437 455 C91134 388 420 446 484 515 534 568 587 C91136 418 443 437 448 466 503 513 540 C91137 286 293 309 317 339 331 323 344 Covance 6329-212 3M T-6316.1 PAGE: 56 WEEK 65 WEEK 69 751 734 802 824 954 990 647 670 841 875 650 667 928 949 661 672 818 842 744 771 737 747 673 699 747 757 696 729 435 442 440 456 500 514 621 659 451 471 331 336 514 525 596 472 545 564 360 382 1282 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 1 - 0 PPM C91138 291 296 298 301 307 298 306 326 C91139 380 404 412 431 446 463 478 355 C91141 354 354 376 413 429 443 444 440 C91142 459 474 483 518 535 532 537 531 C91144 290 280 283 293 298 302 305 305 C91146 395 411 435 457 471 488 514 490 C91147 411 424 440 456 470 492 509 518 C91148 339 347 350 361 383 397 413 436 C91149 316 327 343 359 364 387 389 390 C91150 352 382 398 420 448 458 471 479 C91151 478 497 534 573 593 625 635 646 C91152 341 360 378 398 411 426 C91153 488 511 553 582 617 651 671 694 C91154 413 403 423 440 457 465 488 505 C91156 426 419 417 472 495 508 524 548 C91157 302 312 318 325 332 336 345 351 C91158 359 406 428 475 485 492 506 528 C91159 333 346 356 368 386 400 425 442 C91161 370 399 431 440 461 472 405 244 C91164 442 456 489 525 540 547 579 606 C91166 370 374 380 406 415 423 434 444 C91167 385 383 416 437 466 491 501 527 C91168 337 339 350 369 396 411 415 422 C91170 463 479 505 536 544 570 581 566 C91172 325 335 336 351 365 379 381 397 C91173 338 330 349 376 382 364 275 C91175 474 473 497 539 543 559 579 589 C91176 446 463 471 501 512 523 555 571 C91177 336 336 374 364 388 407 421 446 C91178 362 367 385 416 425 442 438 442 C91179 396 407 424 461 Covance 6329-212 3M T-6316.1 PAGE: 57 WEEK 65 WEEK 69 323 324 440 442 539 542 308 309 521 470 538 536 441 456 390 393 463 467 655 560 710 734 518 528 564 572 355 363 549 565 448 466 622 659 460 478 524 560 419 428 544 538 397 415 595 609 588 603 475 506 435 453 1283 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 1 - 0 PPM C91180 427 435 437 462 495 528 522 571 C91182 423 457 472 510 522 541 580 603 C91183 387 389 398 428 442 457 481 490 C91184 409 430 465 483 509 543 571 613 C91185 450 440 493 525 554 563 558 451 C91186 357 351 360 386 402 417 431 452 C91187 406 413 435 461 486 515 516 492 C91188 326 340 348 372 388 397 419 437 C91189 275 276 282 286 298 303 306 318 C91190 323 327 327 346 357 362 375 382 GROUP: FEMALE 2 - 3 PPM C91191 440 433 470 507 493 521 536 519 C91193 315 320 328 359 372 381 394 413 C91194 395 399 403 425 447 451 424 405 C91195 388 409 420 443 464 473 472 487 C91196 359 357 378 417 450 464 473 502 C91197 391 412 426 443 472 490 485 505 C91199 343 352 357 372 392 412 415 439 C91200 315 327 348 340 363 369 380 387 C91201 348 364 373 392 408 410 414 432 C91202 413 435 448 481 496 522 547 570 C91203 415 430 455 465 478 508 514 535 C91204 371 391 407 436 458 477 453 372 C91206 435 442 449 447 468 489 491 513 C91207 354 358 362 372 389 395 382 403 C91208 338 347 354 368 378 399 409 416 C91209 339 343 352 359 376 393 393 400 C91210 349 365 379 403 422 431 446 477 C91211 389 398 431 456 467 480 495 504 Covance 6329-212 3M T-6316.1 PAGE: 58 WEEK 65 WEEK 69 632 666 504 522 634 651 302 458 471 440 374 447 467 318 327 375 390 562 600 423 442 415 436 506 543 537 571 496 521 454 470 402 426 455 647 592 576 556 561 473 403 401 401 413 365 397 394 484 493 523 551 1284 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 2 - 3 PPM C91212 341 354 372 385 393 416 432 452 C91213 382 380 409 436 456 477 481 499 C91214 364 395 416 427 439 452 462 471 C91215 320 328 350 349 348 344 C91216 331 330 338 350 354 357 361 361 C91217 319 319 330 348 355 372 385 408 C91218 315 320 338 351 382 364 377 399 C91219 336 329 379 386 397 415 414 433 C91220 373 374 396 412 434 448 454 483 C91221 384 387 399 422 439 452 456 480 C91224 300 302 322 340 337 344 370 386 C91225 338 341 340 357 352 357 373 389 C91226 433 438 433 429 434 446 462 484 C91227 356 365 372 380 402 421 427 434 C91228 347 352 359 377 390 393 398 418 C91229 309 310 321 325 337 344 349 358 C91231 422 427 429 470 478 501 519 530 C91232 292 299 305 314 322 318 336 356 C91234 515 503 533 586 632 648 684 719 C91235 291 295 306 316 329 340 343 356 C91236 291 299 309 315 321 338 348 368 C91238 322 340 350 371 396 408 420 437 C91239 371 377 382 394 399 406 418 C91240 340 352 354 351 363 388 382 401 C91242 308 312 313 325 348 333 354 358 C91243 394 408 421 428 445 451 455 467 C91244 319 312 315 335 340 350 367 386 C91245 401 391 415 442 454 474 483 516 C91246 327 338 349 362 374 395 414 421 C91247 428 436 455 489 511 522 527 548 C91249 416 441 461 490 515 544 565 593 Covance 6329-212 3M T-6316.1 PAGE: 59 WEEK 65 WEEK 69 361 514 528 485 505 358 358 424 410 427 436 450 494 501 494 498 391 411 399 404 501 509 453 456 418 440 363 380 555 583 368 375 679 369 369 372 375 343 374 389 369 384 480 494 382 520 530 430 447 484 402 615 631 1285 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 2 - 3 PPM C91250 281 288 294 304 320 345 365 397 GROUP: FEMALE 3 - 30 PPM C91252 386 373 390 428 452 475 495 497 C91253 340 354 361 383 404 417 420 440 C91254 289 299 299 309 322 334 339 357 C91255 333 335 342 354 365 372 378 394 C91256 320 337 351 356 362 372 385 394 C91257 315 321 327 344 358 366 381 398 C91258 354 360 376 397 411 414 415 436 C91259 378 386 402 432 449 432 361 318 C91260 418 433 449 475 491 512 529 552 C91261 321 321 333 360 368 385 401 408 C91262 267 273 293 298 316 325 338 355 C91263 414 424 436 461 472 487 500 506 C91264 320 315 332 341 353 364 381 385 C91265 334 336 354 356 374 391 399 418 C91266 379 388 397 416 427 430 448 467 C91267 297 312 333 355 365 380 378 393 C91268 345 363 376 394 415 438 440 450 C91269 389 397 429 437 461 468 486 497 C91270 321 341 356 387 333 340 296 C91271 335 350 362 382 398 416 440 477 C91273 337 343 356 371 385 389 407 409 C91274 349 361 382 402 407 432 441 469 C91275 407 411 429 463 485 484 462 412 C91276 302 297 312 316 318 323 328 334 C91277 311 316 326 336 338 346 360 371 C91278 331 341 346 363 373 380 389 389 C91279 492 504 515 534 552 582 571 598 Covance 6329-212 3M T-6316.1 PAGE: 60 WEEK 65 WEEK 69 437 473 520 537 454 470 373 385 390 392 397 407 406 395 440 455 539 446 416 424 363 380 440 391 392 402 436 435 482 496 422 459 477 526 569 491 525 418 429 465 470 333 310 336 342 384 418 398 402 611 609 1286 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 3 - 30 PPM C91280 342 349 364 374 386 406 422 441 C91282 424 439 477 506 442 276 C91283 350 379 389 417 422 437 465 485 C91284 343 334 353 374 367 390 402 419 C91286 281 278 304 297 307 323 339 337 C91289 311 315 333 352 360 340 305 281 C91290 379 378 393 401 390 369 311 C91292 382 372 394 421 441 473 507 532 C91294 352 331 337 369 361 369 371 398 C91295 340 320 327 370 377 390 408 424 C91296 274 274 283 294 295 302 303 311 C91297 348 368 377 386 386 397 405 431 C91298 311 316 317 320 332 319 314 338 C91300 372 378 392 411 418 422 432 400 C91301 357 365 392 418 437 461 485 501 C91302 345 357 369 383 399 404 371 359 C91303 422 434 428 470 477 501 518 537 C91305 415 417 449 482 499 525 533 561 C91306 320 320 327 332 342 345 361 372 C91307 357 365 368 376 391 399 412 423 C91308 334 340 359 373 387 403 392 390 C91309 400 413 426 441 457 477 482 513 C91310 323 327 359 379 395 404 419 431 GROUP: FEMALE 4 - 100 PPM C91311 271 276 287 284 288 298 303 302 C91312 267 272 272 275 282 284 283 288 C91313 296 306 322 335 348 357 375 385 C91314 285 291 295 302 316 323 326 335 C91315 283 291 299 315 332 341 354 364 Covance 6329-212 3M T-6316.1 PAGE: 61 WEEK 65 WEEK 69 456 467 498 497 434 459 343 362 279 265 551 585 407 416 442 460 314 322 411 421 304 319 356 335 523 549 308 274 557 584 570 588 379 386 433 447 385 327 477 445 450 287 289 388 390 342 354 374 382 1287 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 4 - 100 PPM C91319 319 325 340 340 353 356 359 364 C91320 307 315 327 343 360 375 394 404 C91321 310 312 332 346 366 384 404 420 C91323 323 330 349 354 369 373 C91324 272 275 271 277 284 286 295 291 C91325 302 304 314 313 313 302 315 328 C91326 243 252 257 264 262 267 278 283 C91327 291 289 299 295 297 304 318 326 C91328 303 302 313 317 327 334 333 344 C91330 317 323 337 351 364 372 393 418 C91331 390 406 422 438 460 481 504 509 C91332 263 266 274 274 276 284 289 295 C91334 319 334 357 374 392 412 422 430 C91335 290 287 304 296 309 313 320 329 C91336 265 250 277 279 284 292 299 302 C91338 244 243 253 241 269 265 263 260 C91339 314 323 334 348 355 365 375 386 C91340 344 340 348 353 358 383 388 398 C91341 323 328 337 335 347 354 367 371 C91342 299 306 313 321 324 349 344 353 C91343 296 293 291 293 298 297 C91344 297 291 296 310 310 316 324 332 C91346 356 356 386 403 406 418 433 437 C91347 300 297 304 305 316 317 C91348 266 257 271 281 280 286 C91349 306 306 268 305 307 310 324 C91351 339 342 346 373 373 397 410 443 C91352 341 352 359 369 391 408 C91353 274 297 303 302 334 344 C91354 257 255 267 272 275 277 285 285 C91356 300 299 313 322 333 347 385 417 Covance 6329-212 3M T-6316.1 PAGE: 62 WEEK 65 WEEK 69 363 334 426 433 427 436 300 301 337 358 288 297 329 339 428 447 502 481 294 310 431 425 341 344 305 308 268 289 403 400 409 416 376 382 378 391 333 340 439 391 468 479 269 269 412 430 1288 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 4 - 100 PPM C91357 247 250 248 248 250 257 258 264 C91358 338 341 353 377 387 403 411 430 C91359 295 267 295 301 311 323 333 340 C91360 263 268 270 276 279 285 289 301 C91361 307 306 313 320 320 322 327 329 C91362 323 329 342 358 376 404 415 433 C91363 353 355 346 358 361 375 377 399 C91364 276 271 278 282 295 290 299 305 C91365 242 243 249 252 259 260 C91366 333 340 351 360 375 400 412 436 C91367 344 306 329 339 320 333 C91368 274 284 285 281 290 294 C91369 319 325 340 346 359 365 377 402 C91371 353 366 377 385 399 411 C91372 307 311 323 334 336 343 351 367 C91373 336 349 359 367 374 379 369 381 C91374 308 319 330 338 349 375 376 394 C91375 291 294 300 309 313 317 324 334 C91376 282 287 291 293 294 306 304 308 C91377 316 330 333 342 352 367 387 427 C91378 276 279 290 290 294 285 286 283 C91380 311 313 321 325 323 317 327 328 GROUP: FEMALE 6 - 100 PPM C91451 286 288 299 308 312 315 346 369 C91452 337 346 354 374 381 394 423 470 C91453 317 326 341 351 358 360 387 423 C91454 277 284 287 291 293 289 306 335 C91455 288 288 300 301 314 321 342 366 C91456 290 293 301 309 309 314 340 371 Covance 6329-212 3M T-6316.1 PAGE: 63 WEEK 65 WEEK 69 288 270 452 440 347 369 304 312 340 351 440 464 407 413 308 315 449 465 425 466 371 371 377 253 407 429 335 357 315 304 455 495 291 295 325 319 382 402 493 543 426 464 338 337 367 377 403 431 1289 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 6 - 100 PPM C91457 325 329 340 348 354 362 379 414 C91458 287 284 297 302 321 324 340 351 C91459 322 327 321 331 337 334 380 408 C91460 244 243 246 249 256 255 246 282 C91461 401 419 436 451 472 500 527 577 C91462 349 347 359 379 386 391 415 432 C91463 290 301 306 309 315 327 349 386 C91464 263 269 271 272 279 290 302 324 C91465 307 313 324 333 338 349 388 408 C91466 346 353 366 375 390 412 426 465 C91467 284 291 293 303 310 322 341 359 C91468 272 280 280 285 270 286 288 298 C91469 287 290 297 303 306 308 322 346 C91470 349 354 373 382 401 412 434 462 C91471 304 318 331 346 363 390 410 430 C91472 314 323 337 347 369 388 416 461 C91473 273 280 281 290 297 307 314 343 C91474 319 321 339 337 342 353 367 420 C91475 343 357 363 385 388 386 386 456 C91476 322 327 335 344 351 361 374 424 C91477 322 327 338 348 367 373 392 423 C91478 332 337 347 360 365 386 418 456 C91479 343 353 368 390 382 396 430 466 C91480 291 296 306 313 322 335 340 367 C91481 267 266 278 303 313 323 341 381 C91482 312 317 326 333 332 346 359 401 C91483 310 316 324 330 337 348 349 370 C91484 296 302 312 323 326 345 348 382 C91485 279 279 293 295 296 299 318 341 C91486 300 310 318 324 330 350 361 402 C91487 310 311 318 326 332 349 386 430 Covance 6329-212 3M T-6316.1 PAGE: 64 WEEK 65 WEEK 69 431 454 443 489 609 610 445 463 399 407 330 324 420 439 492 503 377 378 353 320 460 297 450 446 494 514 361 393 420 448 483 528 437 454 441 450 473 505 493 507 369 383 394 354 427 453 385 398 344 263 349 365 419 446 478 496 1290 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 6 - 100 PPM C91488 336 343 352 365 365 373 377 412 C91489 305 318 337 347 351 368 391 436 C91490 291 302 314 326 337 361 381 413 Covance 6329-212 3M T-6316.1 PAGE: 65 WEEK 65 WEEK 69 433 458 464 498 417 409 1291 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: C90711 C90712 C90713 C90715 C90716 C90720 C90722 C90724 C90726 C90727 C90728 C90729 C90733 C90734 C90735 C90738 C90739 C90741 C90742 C90743 C90744 C90745 C90747 C90749 C90750 C90755 C90758 C90759 C90760 C90761 C90762 C90763 MALE 1 - 0 PPM 674 516 704 720 847 850 868 885 815 838 977 987 793 812 872 893 723 700 852 860 948 969 819 846 732 644 915 807 743 745 722 718 783 792 828 840 772 795 978 1004 851 862 709 502 696 636 740 741 784 797 1057 1067 821 834 709 664 878 821 785 765 715 719 807 818 705 838 860 842 978 811 902 691 845 949 845 658 746 715 788 834 797 1019 845 618 731 811 1051 589 538 702 782 836 703 829 854 862 945 830 931 690 843 932 856 633 769 712 796 844 803 1047 759 606 705 835 1042 757 387 670 802 702 683 752 784 879 866 830 956 671 803 926 838 645 761 690 807 873 810 1069 580 653 829 1019 799 802 675 666 685 889 851 962 647 720 931 604 673 691 830 805 809 1074 568 460 841 994 788 807 636 588 915 862 977 660 485 937 685 673 849 654 819 1094 540 854 960 781 555 924 890 974 662 937 689 749 818 1090 511 864 781 Covance 6329-212 3M T-6316.1 PAGE: 66 WEEK 105 434 923 886 981 634 909 712 816 1089 488 856 739 1292 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 1 - 0 PPM C90764 C90765 C90770 C90771 C90776 C90777 C90778 C90779 727 900 708 717 1013 931 680 830 718 908 706 734 1029 902 686 813 GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90790 C90791 C90793 C90794 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90809 815 797 659 705 831 716 885 931 896 719 756 931 870 705 836 832 714 743 860 691 842 813 676 696 813 721 908 947 820 732 685 902 892 709 823 758 664 709 857 664 712 932 705 723 1027 848 678 796 833 772 679 666 782 723 897 960 640 735 900 907 706 789 783 445 652 857 681 925 647 692 1031 781 686 770 849 763 653 639 806 741 915 992 759 884 919 712 792 641 844 661 897 536 594 1031 670 674 851 768 655 588 805 736 920 987 774 857 908 705 841 616 815 671 907 545 1044 676 459 839 756 672 495 801 719 918 1010 788 852 923 702 865 598 778 696 906 1029 699 837 710 667 774 941 1033 811 879 914 704 890 736 717 922 704 826 709 675 693 871 1059 813 839 782 709 641 734 Covance 6329-212 3M T-6316.1 PAGE: 67 WEEK 105 930 739 770 701 686 510 634 1070 796 795 707 538 743 1293 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 2 - 3 PPM C90810 C90811 C90812 C90815 C90816 C90819 C90821 C90822 C90823 C90824 C90825 C90826 C90828 C90829 C90830 C90832 C90835 C90837 C90838 C90839 C90840 977 813 784 757 944 1002 990 695 789 845 875 887 738 1080 794 803 764 439 1101 1037 984 1000 824 779 767 883 1012 991 512 786 825 879 886 695 1105 805 850 767 1110 1002 987 GROUP: MALE 3 - 30 PPM C90841 C90842 C90844 C90845 C90847 C90848 C90849 795 608 851 873 772 986 660 817 615 860 780 775 499 979 839 775 774 831 1005 1006 790 684 876 874 1127 797 829 770 1118 866 988 823 625 879 778 977 838 780 764 655 1026 1004 811 886 896 1147 828 846 757 1126 819 892 832 629 852 727 990 839 762 742 1031 1023 808 871 888 1172 838 854 740 1108 808 895 740 617 853 682 999 848 747 741 1061 800 875 1190 842 860 755 1088 725 893 750 605 850 636 999 856 703 742 1100 784 869 1202 848 856 755 1075 714 880 527 592 835 520 984 851 623 713 787 1224 857 877 718 1026 873 581 799 Covance 6329-212 3M T-6316.1 PAGE: 68 WEEK 105 944 877 524 670 799 1219 822 906 677 963 841 592 673 1294 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 3 - 30 PPM C90850 752 767 782 783 789 776 672 637 C90851 760 765 778 776 781 796 819 831 C90854 797 798 812 816 808 818 816 803 C90855 797 801 805 817 799 811 821 816 C90856 852 856 869 890 899 923 936 946 C90857 736 719 704 720 708 711 665 557 C90858 725 717 709 692 682 680 662 646 C90860 574 578 580 597 593 600 613 609 C90864 714 714 719 734 724 728 739 740 C90865 664 679 689 695 708 735 748 750 C90867 588 593 599 604 610 618 612 616 C90868 667 C90869 878 903 914 914 929 943 928 910 C90870 803 675 C90871 706 709 719 734 740 743 755 754 C90872 757 773 789 799 735 C90873 640 C90875 807 826 826 835 842 C90879 752 657 448 C90881 917 926 951 950 940 976 1008 1026 C90882 874 899 917 921 936 959 1004 1016 C90883 657 670 674 691 676 679 682 680 C90884 633 637 635 633 647 681 697 675 C90885 835 841 836 846 825 866 794 788 C90886 885 910 840 694 573 C90888 721 689 439 C90889 743 769 751 765 778 822 835 849 C90892 578 422 C90893 827 838 835 855 864 892 930 954 C90894 805 797 785 806 785 781 751 C90895 752 759 765 780 782 781 Covance 6329-212 3M T-6316.1 PAGE: 69 WEEK 105 606 832 775 818 946 614 609 756 733 619 924 752 1014 1016 653 626 781 859 944 1295 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 3 - 30 PPM C90897 897 917 914 914 930 953 C90899 682 696 695 702 716 738 748 656 C90900 712 716 716 713 711 709 727 696 GROUP: MALE 4 - 100 PPM C90901 700 C90903 687 699 713 712 724 739 746 663 C90904 613 619 631 632 632 621 612 615 C90906 801 806 816 826 850 872 864 740 C90908 748 760 743 768 777 785 791 811 C90910 722 729 742 751 754 768 791 819 C90911 717 716 731 733 733 734 738 749 C90912 524 507 429 C90914 861 891 914 938 914 868 777 C90915 851 885 891 901 912 906 C90916 706 712 717 723 730 750 753 749 C90919 655 657 C90920 736 763 771 759 754 794 827 813 C90923 729 742 717 552 C90925 732 760 780 792 780 643 C90926 835 854 858 887 853 855 749 684 C90927 703 716 742 746 755 769 772 787 C90928 662 667 683 607 493 522 481 496 C90930 683 688 699 643 597 C90931 742 747 763 744 745 761 710 C90933 736 732 728 743 745 765 792 814 C90934 668 675 679 701 704 719 727 692 C90936 695 714 740 746 720 746 735 661 C90937 661 664 653 659 643 630 594 520 C90941 905 848 728 605 Covance 6329-212 3M T-6316.1 PAGE: 70 WEEK 105 699 601 833 845 758 770 442 801 527 842 1296 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 4 - 100 PPM C90943 C90944 C90946 C90947 C90948 C90953 C90955 C90956 C90958 C90959 C90963 C90964 C90965 C90968 C90969 C90970 686 603 615 725 666 724 756 736 748 798 450 532 527 715 894 729 657 609 485 730 653 729 748 756 694 785 538 744 890 742 GROUP: MALE 6 - 100 PPM C91042 C91043 C91047 C91048 C91050 C91051 C91053 C91054 C91055 C91056 C91057 C91058 814 817 633 800 818 852 686 976 1034 754 624 745 830 859 659 712 828 870 690 982 1066 775 759 650 618 709 640 729 774 789 717 806 547 762 739 755 824 893 649 838 872 662 1005 989 778 784 659 630 698 657 750 786 811 709 756 543 652 653 771 849 941 664 860 733 1028 769 580 636 698 663 756 790 826 701 545 677 772 847 663 863 492 1061 771 635 691 653 779 789 841 683 556 659 784 690 655 858 1067 777 640 667 655 780 787 836 542 655 799 687 767 1088 777 654 617 649 762 787 820 557 603 692 760 1105 773 Covance 6329-212 3M T-6316.1 PAGE: 71 WEEK 105 669 550 655 766 793 630 570 645 720 1084 766 1297 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 6 - 100 PPM C91059 870 866 666 C91060 761 787 800 816 812 817 829 831 C91061 755 763 773 801 788 C91062 786 780 593 551 405 335 C91063 567 578 595 607 617 570 439 C91064 838 853 866 888 889 891 915 C91065 650 669 685 674 682 708 734 763 C91066 687 533 480 417 C91067 838 831 840 857 853 875 836 C91069 1046 1088 1129 C91070 686 687 695 690 682 676 667 643 C91071 904 927 949 974 980 998 1014 1000 C91072 666 666 669 651 623 577 590 565 C91073 989 1005 990 877 919 954 972 993 C91074 708 734 732 703 722 744 759 719 C91075 836 736 C91076 789 800 820 774 778 760 787 778 C91077 770 735 713 C91078 703 713 709 700 690 642 C91079 719 683 609 463 C91080 754 769 780 802 819 810 747 GROUP: FEMALE 1 - 0 PPM C91123 453 464 484 502 521 530 533 C91125 482 490 505 526 526 490 C91127 538 540 538 554 553 555 557 530 C91128 663 C91130 473 381 C91131 343 351 352 365 361 372 368 374 C91132 518 484 493 528 464 Covance 6329-212 3M T-6316.1 PAGE: 72 WEEK 105 833 759 621 955 500 1027 795 365 358 1298 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 1 - 0 PPM C91134 332 C91136 581 609 618 582 482 C91137 406 442 451 468 481 494 501 458 C91138 316 322 322 343 348 370 372 400 C91141 447 468 C91142 367 C91144 313 317 313 325 326 336 333 336 C91146 395 C91147 482 336 C91148 465 480 494 516 523 553 587 623 C91149 396 392 406 394 C91150 431 C91151 367 C91153 754 737 716 C91154 529 509 C91156 576 C91157 371 369 372 389 384 393 404 401 C91158 572 598 626 658 684 715 C91159 479 490 528 548 541 575 585 586 C91164 687 703 714 664 628 563 C91166 491 511 519 540 547 566 587 598 C91167 583 604 617 617 611 636 656 684 C91168 433 441 467 C91170 540 512 418 343 262 C91172 430 425 419 411 405 436 458 483 C91175 638 650 662 590 C91176 632 637 662 692 707 729 672 602 C91177 545 576 595 619 644 652 C91178 453 475 481 483 410 C91182 693 709 600 425 C91183 538 549 562 586 589 605 616 620 Covance 6329-212 3M T-6316.1 PAGE: 73 WEEK 105 441 349 631 422 613 612 489 458 645 1299 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 1 - 0 PPM C91184 675 699 700 704 594 C91186 486 484 494 515 502 443 461 C91187 298 C91188 507 505 528 539 545 565 602 609 C91189 344 347 355 355 356 324 C91190 405 401 408 407 407 417 437 428 GROUP: FEMALE 2 - 3 PPM C91191 629 640 661 661 628 664 553 C91193 463 457 466 458 389 C91194 471 480 483 486 471 442 441 372 C91195 561 573 595 613 611 570 468 C91196 596 614 497 C91197 538 542 546 560 558 578 603 595 C91199 492 504 533 534 534 469 377 C91200 422 445 452 458 C91201 474 495 500 500 511 521 492 429 C91202 608 637 659 644 C91203 590 C91206 382 C91207 406 420 431 434 426 416 449 438 C91208 340 C91209 424 C91210 498 C91211 563 553 562 572 578 596 609 607 C91213 488 399 C91214 536 536 545 540 535 571 548 C91216 370 370 372 366 371 380 387 396 C91218 448 368 450 361 345 385 332 C91219 459 476 460 365 Covance 6329-212 3M T-6316.1 PAGE: 74 WEEK 105 605 412 620 407 452 603 403 1300 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 2 - 3 PPM C91220 523 536 559 573 561 602 639 667 C91221 519 531 548 558 588 622 641 648 C91224 426 415 433 444 440 409 368 C91225 422 438 443 C91226 512 426 416 435 C91227 481 495 504 506 513 516 522 538 C91228 458 458 477 493 451 392 C91229 396 338 392 362 372 303 C91231 598 610 625 644 642 C91232 391 392 406 396 403 419 420 434 C91235 381 404 422 412 430 433 462 477 C91236 386 393 409 420 434 446 462 477 C91240 415 438 C91242 412 409 427 427 430 449 447 461 C91243 516 521 547 562 577 592 612 611 C91245 558 570 595 603 587 542 C91246 454 431 431 437 C91247 401 C91249 665 685 705 715 709 563 409 C91250 505 GROUP: FEMALE 3 - 30 PPM C91252 548 554 560 580 577 611 625 630 C91253 479 485 496 517 527 527 544 558 C91254 382 389 386 398 395 397 421 435 C91255 400 C91256 414 420 430 441 439 452 424 420 C91257 378 333 283 C91258 462 463 462 326 C91260 326 Covance 6329-212 3M T-6316.1 PAGE: 75 WEEK 105 675 628 575 434 490 472 474 604 637 577 434 423 1301 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 3 - 30 PPM C91261 437 453 468 472 468 435 433 C91262 400 405 407 395 282 C91264 412 404 419 434 430 449 470 477 C91265 450 464 466 481 461 363 C91266 515 523 534 545 561 570 524 468 C91268 512 517 547 562 580 601 619 C91269 593 521 410 C91271 548 561 539 443 404 359 C91273 426 424 360 C91276 339 342 340 362 352 393 420 C91278 410 422 427 423 426 432 445 444 C91279 602 612 590 583 572 563 569 588 C91280 484 492 497 501 505 509 419 C91283 525 517 541 534 386 311 C91284 471 465 481 478 481 499 504 C91286 367 366 378 380 380 398 394 410 C91289 227 C91292 610 629 649 666 684 715 634 775 C91294 434 468 456 471 472 496 515 516 C91295 490 495 519 528 538 562 565 551 C91296 335 335 311 256 C91297 421 417 433 429 446 C91298 342 C91300 316 317 311 C91301 570 584 601 623 645 666 687 697 C91303 591 607 617 621 610 634 640 645 C91305 608 612 617 603 C91306 391 397 413 404 406 418 413 460 C91307 456 464 483 490 503 516 517 474 C91310 476 494 511 529 535 534 549 543 Covance 6329-212 3M T-6316.1 PAGE: 76 WEEK 105 424 448 573 415 782 491 491 705 626 427 558 1302 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 4 - 100 PPM C91312 293 293 302 309 298 284 217 C91313 392 392 380 370 C91314 341 C91315 395 405 414 419 421 439 451 470 C91320 428 353 C91321 471 473 491 486 459 427 C91324 308 314 327 331 323 332 336 337 C91325 366 379 386 406 424 433 424 364 C91326 292 275 237 221 C91327 353 354 367 400 405 469 495 513 C91330 474 501 501 418 C91331 465 327 C91332 314 320 327 340 336 341 336 315 C91334 425 431 422 422 417 403 315 348 C91335 359 362 369 378 386 409 410 402 C91336 320 318 330 337 336 346 345 354 C91338 281 297 292 312 310 311 317 330 C91339 423 425 438 454 443 404 327 C91340 413 408 409 405 397 403 390 323 C91341 392 397 398 415 430 445 460 458 C91342 393 405 416 423 427 448 459 462 C91344 350 355 369 377 381 394 375 355 C91351 514 536 520 467 454 385 C91354 260 255 247 242 C91356 443 471 523 531 510 486 456 391 C91357 292 288 288 301 299 306 325 315 C91358 427 C91359 389 399 C91360 319 321 327 335 330 346 352 345 C91361 354 365 370 365 364 373 361 360 C91362 500 519 535 576 604 644 657 668 Covance 6329-212 3M T-6316.1 PAGE: 77 WEEK 105 510 348 532 335 424 359 338 318 468 469 316 326 359 369 675 1303 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 4 - 100 PPM C91363 434 434 428 452 446 475 296 C91364 323 312 317 293 271 274 271 276 C91366 485 484 466 402 C91369 484 C91372 388 386 401 400 394 394 408 400 C91374 435 439 436 433 405 367 300 C91375 372 385 358 C91376 323 322 331 335 330 332 336 340 C91378 296 304 305 312 306 304 297 300 C91380 333 324 311 329 320 340 413 421 GROUP: FEMALE 6 - 100 PPM C91451 406 419 447 454 471 474 496 485 C91452 581 C91453 490 498 527 524 559 561 446 361 C91454 350 373 337 C91455 385 401 410 429 445 450 452 460 C91456 443 351 C91457 473 505 506 534 553 578 C91459 517 553 565 563 586 600 614 580 C91461 578 541 493 404 C91462 491 508 510 518 531 531 524 528 C91463 433 438 436 430 C91464 293 C91465 465 480 355 269 239 C91466 539 571 622 632 607 473 C91467 395 419 423 445 444 454 491 500 C91471 458 462 446 441 425 418 310 C91472 546 551 584 603 631 656 641 C91473 408 420 446 460 485 493 513 524 Covance 6329-212 3M T-6316.1 PAGE: 78 WEEK 105 397 354 316 448 482 348 454 586 513 511 529 1304 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 6 - 100 PPM C91474 458 473 494 453 371 315 C91475 554 598 609 610 630 671 678 C91476 451 387 C91477 463 478 416 339 C91478 518 534 564 539 C91479 518 539 556 557 569 582 607 630 C91480 389 400 420 432 438 452 462 456 C91481 317 C91482 463 463 416 371 309 284 C91483 409 427 438 440 446 434 C91485 382 391 397 415 418 441 464 473 C91486 470 446 359 357 263 C91487 530 563 604 608 636 669 690 708 C91488 462 479 488 476 502 496 521 521 C91489 535 550 583 C91490 437 477 459 Covance 6329-212 3M T-6316.1 PAGE: 79 WEEK 105 638 462 474 726 537 1305 APPENDIX 4 Covance 6329-212 3M T-6316.1 In d iv id u a l F o o d C o n su m p tio n D a ta (g) In d iv id u a l T est M a terial C o n su m p tio n D a ta (m g /k g /d ay ) 1306 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK WEEK 910 GROUP: MALE 1 - 0 PPM C90711 C90712 C90713 C90714 C90715 C90716 C90717 C90718 C90719 C90720 C90721 C90722 C90723 C90724 C90725 C90726 C90727 C90728 C90729 C90730 C90731 C90732 C90733 C90734 C90735 C90736 C90737 C90738 C90739 C90740 C90741 175 162 177 160 182 157 171 SPILLED 156 176 188 169 182 189 170 162 181 165 176 184 179 200 193 230 175 228 161 185 165 155 152 177 163 182 165 180 167 165 162 160 186 192 196 196 183 173 172 191 169 183 184 197 199 198 225 177 SPILLED 168 176 174 168 160 188 187 193 201 178 171 181 169 167 194 211 SPILLED 201 182 177 175 195 177 190 195 206 209 197 226 182 181 176 204 SPILLED 172 166 176 178 222 184 178 168 187 174 NOT TAKEN 195 235 213 209 191 NOT TAKEN 183 207 172 192 190 214 NOT TAKEN 209 221 184 190 180 184 183 196 175 177 173 218 183 175 163 188 170 196 253 172 213 189 191 201 176 192 185 212 202 217 187 186 186 215 185 177 183 179 174 230 189 186 166 188 176 197 248 SPILLED 217 189 189 206 178 194 196 206 199 224 181 190 186 193 190 177 187 186 180 224 195 194 169 190 177 199 257 161 218 179 SPILLED 207 179 183 193 212 202 220 176 199 184 191 186 177 182 180 175 218 197 185 165 NOT TAKEN NOT TAKEN 192 260 170 214 178 SPILLED 208 177 188 196 216 200 223 177 198 NOT TAKEN 199 190 186 182 176 183 206 182 189 166 195 SPILLED 163 218 166 SPILLED 204 176 184 185 209 191 213 169 185 200 173 175 179 172 175 194 185 184 159 191 178 162 208 165 187 201 174 180 183 205 197 218 178 183 195 175 180 174 1307 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK WEEK 910 GROUP: MALE 1 - 0 PPM C90742 C90743 C90744 C90745 C90746 C90747 C90748 C90749 C90750 C90751 C90752 C90753 C90754 C90755 C90756 C90757 C90758 C90759 C90760 C90761 C90762 C90763 C90764 C90765 C90766 C90767 C90768 C90769 C90770 C90771 C90772 159 171 180 163 165 167 193 158 163 SPILLED 201 171 188 187 146 181 171 163 185 171 177 188 172 197 162 154 169 175 180 177 182 164 180 187 172 178 176 196 167 171 181 211 183 197 193 152 178 177 174 199 172 188 186 186 200 167 159 182 182 171 180 199 170 185 194 172 186 177 200 165 174 181 206 182 208 194 160 184 183 183 SPILLED 165 182 195 187 207 190 169 184 181 172 176 SPILLED 177 197 202 181 199 176 202 169 177 184 216 NOT TAKEN 230 195 165 192 202 190 194 176 183 193 197 204 178 166 188 183 189 183 191 182 198 191 177 201 183 205 161 171 184 206 SPILLED 200 162 189 200 189 210 174 182 202 192 200 176 176 184 178 188 186 192 181 196 191 178 201 170 208 158 160 182 220 SPILLED 204 165 187 224 190 194 174 178 197 197 206 180 177 204 180 SPILLED 183 193 178 192 193 181 204 180 209 166 165 SPILLED 222 SPILLED 209 166 189 SPILLED 191 SPILLED 177 201 202 SPILLED 211 180 179 214 183 SPILLED 184 191 177 197 186 174 SPILLED 179 201 162 169 SPILLED SPILLED SPILLED 210 NOT TAKEN 189 226 185 193 175 169 195 205 205 178 174 176 NOT TAKEN 178 177 192 170 193 201 174 199 170 193 166 170 SPILLED 215 214 213 186 215 176 196 174 179 199 SPILLED 208 164 199 176 155 175 179 174 192 210 175 202 172 200 170 163 200 210 228 213 187 205 173 180 176 165 194 182 202 177 178 171 172 173 175 1308 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 1 - 0 PPM C90773 C90774 C90775 C90776 C90777 C90778 C90779 C90780 174 176 176 193 177 167 181 166 184 179 179 185 188 168 192 177 GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 162 SPILLED 145 161 163 177 162 217 176 186 145 165 161 173 168 179 156 188 168 174 169 169 148 167 167 175 167 180 186 190 158 175 169 194 177 185 159 195 171 185 181 179 SPILLED 196 193 171 197 182 192 193 NOT TAKEN 203 206 176 193 189 191 185 199 205 176 189 193 169 182 157 173 172 168 173 188 197 198 157 181 181 195 176 206 165 201 174 SPILLED 172 189 164 175 177 179 181 188 NOT TAKEN 197 168 197 182 198 182 199 168 137 174 185 171 SPILLED 167 174 177 179 187 185 204 177 199 180 214 191 198 172 182 169 185 192 182 206 211 176 188 195 174 198 166 176 184 176 195 181 203 173 193 177 202 183 194 174 176 169 192 197 192 182 179 212 215 215 218 180 173 186 183 201 203 172 SPILLED 234 186 158 164 168 170 187 191 176 175 199 198 181 180 209 210 174 173 187 191 182 180 201 204 192 197 210 214 182 180 195 175 177 176 190 194 Covance 6329-212 3M T-6316.1 PAGE: 3 WEEK WEEK 910 188 188 175 179 205 200 209 202 167 167 181 178 203 198 182 182 205 191 160 159 170 171 186 191 161 163 197 197 178 177 204 206 175 170 192 183 178 171 205 201 194 193 221 219 173 175 197 178 170 165 184 185 1309 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK WEEK 910 GROUP: MALE 2 - 3 PPM C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 169 174 147 160 185 199 190 204 195 198 149 152 172 178 174 183 171 173 173 190 172 172 165 172 150 152 144 151 167 177 177 178 161 161 171 190 183 191 165 163 189 190 138 146 148 152 180 180 175 186 204 SPILLED 160 172 178 195 191 203 175 186 169 180 182 156 195 209 193 150 183 189 173 192 175 174 156 158 183 177 164 185 196 171 194 152 155 191 198 SPILLED 179 203 201 192 178 190 164 200 212 202 164 187 193 180 194 186 177 157 NOT TAKEN 185 192 NOT TAKEN 193 190 172 202 154 161 181 194 SPILLED 182 215 203 193 183 198 160 205 211 205 168 183 202 SPILLED 197 205 173 162 189 188 199 191 178 199 153 158 177 193 SPILLED 185 209 202 201 185 196 162 202 218 208 165 175 198 178 196 198 177 162 193 189 205 200 175 208 156 159 191 195 SPILLED 185 219 199 191 186 200 160 190 222 200 162 176 199 188 198 203 181 154 198 196 SPILLED 193 180 206 163 167 188 195 SPILLED 188 212 206 187 195 205 161 192 212 199 161 177 200 177 191 198 181 NOT TAKEN 196 192 203 192 174 204 159 169 187 190 SPILLED 183 221 203 191 203 203 152 185 213 195 159 173 199 176 191 195 183 194 198 SPILLED 185 176 198 159 171 177 193 SPILLED 184 223 203 188 201 203 154 181 210 197 160 181 198 189 188 202 172 195 200 221 182 178 197 159 175 174 194 245 179 227 195 186 193 1310 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 2 - 3 PPM C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 177 186 179 174 168 163 193 186 167 191 202 192 179 162 163 202 193 177 GROUP: MALE 3 - 30 PPM C90841 C90842 C90843 C90844 C90845 C90846 C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 160 138 167 179 SPILLED 149 159 159 170 154 161 172 SPILLED 184 210 173 170 151 178 164 154 177 186 174 151 165 168 175 156 178 197 149 195 197 182 174 156 188 198 208 202 199 207 196 218 NOT TAKEN 195 NOT TAKEN 175 182 186 183 208 189 161 179 183 181 183 181 168 175 173 179 174 172 203 210 214 212 215 208 198 208 213 217 214 219 183 188 195 193 197 197 164 170 184 189 188 185 132 145 146 142 148 141 173 189 180 191 SPILLED 183 187 SPILLED 190 SPILLED 188 SPILLED 177 179 183 185 183 183 148 NOT TAKEN 168 171 166 170 165 163 175 185 186 185 188 188 178 176 176 183 175 176 164 165 168 167 172 165 172 183 195 193 190 179 179 178 179 183 180 191 164 NOT TAKEN 194 194 SPILLED SPILLED SPILLED SPILLED 194 187 195 185 182 185 174 183 177 178 189 178 168 182 181 184 188 178 152 159 154 159 165 163 199 195 191 190 196 193 Covance 6329-212 3M T-6316.1 PAGE: 5 WEEK WEEK 910 190 195 178 168 213 214 196 184 149 187 200 179 171 195 181 165 SPILLED 178 SPILLED 182 179 182 148 192 188 182 179 164 211 206 192 180 142 189 192 176 163 184 193 159 175 181 195 183 184 182 152 192 1311 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 3 - 30 PPM C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 163 167 158 158 148 159 216 226 176 178 162 158 177 161 160 162 184 174 194 197 184 SPILLED 158 162 156 SPILLED 178 179 175 179 161 165 204 202 157 158 147 154 180 171 192 197 176 182 172 174 182 202 174 173 177 174 190 201 173 174 167 166 168 168 187 185 166 167 156 229 177 165 160 165 184 204 SPILLED 162 166 182 184 172 213 166 158 184 206 189 178 191 183 176 188 177 171 171 186 165 172 169 SPILLED 172 166 NOT TAKEN 162 193 202 SPILLED 168 172 194 189 169 202 174 172 185 196 193 180 SPILLED 171 168 199 176 176 159 NOT TAKEN 170 173 156 226 169 157 165 195 199 192 171 173 201 187 174 196 177 172 178 199 191 185 220 171 167 211 180 171 161 SPILLED 178 163 216 175 160 164 198 189 192 168 179 203 189 170 185 170 171 175 198 182 189 215 174 160 203 174 162 160 166 177 153 215 181 157 171 194 190 189 163 175 201 188 167 197 170 172 184 201 186 188 SPILLED 177 168 202 179 168 165 169 181 156 214 183 159 165 195 187 188 154 172 191 193 169 197 169 179 185 203 181 188 215 179 165 205 184 167 168 Covance 6329-212 3M T-6316.1 PAGE: 6 WEEK 9 WEEK 10 154 157 173 173 152 159 210 204 176 170 155 159 169 163 195 191 185 175 187 193 154 156 172 165 203 198 194 194 178 166 209 204 146 168 174 170 188 186 206 198 188 184 187 185 214 220 173 183 157 159 203 195 180 183 165 165 156 154 1312 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 3 - 30 PPM C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 180 194 130 123 181 181 182 177 191 200 194 SPILLED 181 187 168 169 151 156 166 164 GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 C90915 C90916 C90917 C90918 154 151 165 159 154 150 169 162 156 SPILLED 161 153 155 148 174 169 179 188 162 155 175 167 162 160 169 175 188 184 175 174 142 147 174 174 174 179 196 127 183 169 190 SPILLED 191 168 157 171 147 152 149 166 160 157 153 176 188 161 167 167 171 185 186 150 183 179 NOT TAKEN 132 181 174 171 SPILLED 195 169 156 174 155 162 155 169 168 168 158 180 191 164 167 173 174 188 191 149 180 179 141 185 181 192 181 204 175 157 173 158 161 157 162 168 173 162 179 183 169 173 178 172 189 198 152 185 182 139 146 149 185 188 187 178 185 175 172 180 184 181 SPILLED 169 197 204 199 170 182 179 154 168 167 169 175 184 161 155 150 158 152 155 159 153 148 160 164 159 162 161 165 167 167 164 159 152 150 177 172 171 181 193 198 173 174 175 170 166 164 175 178 169 155 89 140 183 187 182 193 183 190 147 142 150 179 179 181 180 184 188 Covance 6329-212 3M T-6316.1 PAGE: 7 WEEK 9 WEEK 10 148 179 172 199 SPILLED 195 169 163 176 144 145 147 156 162 171 149 167 185 166 172 177 187 182 179 146 172 177 148 174 167 187 209 186 171 153 173 151 150 139 156 164 166 153 171 188 169 171 170 185 182 183 145 159 168 1313 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 4 - 100 PPM C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 C90949 182 162 169 162 176 165 176 169 176 151 151 190 161 163 188 159 182 171 173 156 164 203 199 164 SPILLED 139 157 168 166 206 199 178 162 169 153 167 164 SPILLED 180 181 152 159 179 153 149 188 157 178 165 209 158 157 186 174 164 180 139 157 157 161 173 166 189 165 164 163 171 175 168 185 180 157 150 176 158 154 192 156 177 173 170 162 160 193 180 163 SPILLED 144 156 SPILLED 163 171 156 187 172 159 157 168 172 160 182 184 158 154 175 172 151 197 153 NOT TAKEN 154 176 165 NOT TAKEN 198 SPILLED NOT TAKEN SPILLED 142 156 165 164 173 149 192 169 170 160 166 177 158 185 185 157 157 155 177 142 195 162 170 154 161 197 190 202 138 155 172 163 176 162 185 165 167 158 166 174 160 182 181 156 156 174 181 151 194 160 172 183 160 194 185 SPILLED 140 151 170 165 176 140 185 169 169 153 165 184 155 182 178 157 152 177 172 152 193 154 173 170 165 194 190 188 143 154 164 162 175 131 187 168 170 159 171 184 160 184 174 154 159 179 169 154 193 154 175 160 169 199 187 177 151 156 163 156 177 147 Covance 6329-212 3M T-6316.1 PAGE: 8 WEEK 9 WEEK 10 187 171 165 147 165 173 151 177 169 149 159 179 168 150 182 172 168 SPILLED 165 194 176 167 150 151 150 158 171 129 179 166 170 152 172 181 154 173 162 149 159 180 162 149 184 166 168 169 160 184 180 190 144 142 160 159 163 104 1314 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 4 - 100 PPM C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 177 169 195 154 164 188 165 158 176 162 174 155 189 176 206 165 181 148 147 177 155 177 168 197 156 162 184 161 159 166 163 194 154 188 173 208 166 187 152 150 179 151 GROUP: MALE 5 - 300 PPM C90971 C90972 C90973 C90974 C90975 C90976 C90977 131 129 167 158 176 163 162 110 121 162 147 174 144 144 179 165 206 163 169 186 167 161 169 169 174 SPILLED 185 181 210 166 187 153 150 177 156 170 146 202 171 159 177 168 165 175 164 176 NOT TAKEN 190 184 205 163 NOT TAKEN 166 164 181 169 169 163 193 165 165 174 172 168 181 168 181 190 188 209 159 174 165 182 173 175 179 182 170 173 177 189 192 194 165 167 170 162 168 171 163 166 166 175 180 183 168 128 156 177 166 171 170 173 177 178 195 178 189 185 190 182 186 184 203 201 197 157 135 152 175 166 167 168 167 166 185 181 196 157 SPILLED 160 107 111 110 120 116 NOT TAKEN 125 120 112 108 112 NOT TAKEN 157 157 164 161 151 NOT TAKEN 142 145 138 138 138 NOT TAKEN 178 168 170 174 179 NOT TAKEN 137 137 130 127 130 NOT TAKEN 147 135 SPILLED SPILLED SPILLED NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 9 WEEK 9 WEEK 10 167 169 163 165 180 177 163 157 157 150 165 167 169 110 164 156 155 154 165 168 167 168 178 178 177 176 193 192 147 143 161 160 157 154 189 202 157 155 1315 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 5 - 3 0 0 PPM C90978 C90979 C90980 C90981 C90982 C90983 C90984 C90985 C90986 C90987 C90988 C90989 C90990 C90991 C90992 C90993 C90994 C90995 C90996 C90997 C90998 C90999 C91000 C91001 C91002 C91003 C91004 C91005 C91006 C91007 C91008 159 142 174 172 157 130 167 158 165 158 212 173 161 149 154 163 169 163 173 162 143 157 158 171 139 152 183 190 145 156 SPILLED 154 138 169 165 158 146 154 153 163 148 170 166 157 150 147 155 157 EXCLUDE 161 153 143 148 145 164 133 139 SPILLED 159 138 157 172 SPILLED 131 169 159 158 127 156 136 156 138 155 161 161 147 142 158 157 156 153 141 146 144 136 166 132 134 146 160 132 153 167 145 127 166 151 151 117 NOT TAKEN 131 165 137 133 158 148 155 133 154 156 150 150 138 146 137 130 176 133 137 145 NOT TAKEN 126 155 SPILLED 142 133 169 155 145 109 134 SPILLED 139 SPILLED 156 153 143 134 160 153 152 146 139 153 137 138 174 128 127 SPILLED 128 156 SPILLED 134 127 163 151 156 114 128 149 139 SPILLED 150 154 142 143 152 157 140 148 143 161 135 128 184 131 132 145 125 160 SPILLED 133 137 164 149 148 113 132 147 147 SPILLED 155 153 149 144 145 154 149 150 142 163 139 124 174 128 NOT TAKEN 141 111 156 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 10 WEEK WEEK 910 1316 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 5 - 3 0 0 PPM C91009 C91010 C91011 C91012 C91013 C91014 C91015 C91016 C91017 C91018 C91019 C91020 C91021 C91022 C91023 C91024 C91025 C91026 C91027 C91028 C91029 C91030 C91031 C91032 C91033 C91034 C91035 C91036 C91037 C91038 C91039 179 161 170 143 191 149 166 148 159 180 178 161 151 169 162 135 157 138 163 151 164 133 170 159 158 177 152 172 196 184 176 153 163 133 187 160 158 141 143 171 167 150 159 165 156 132 187 144 138 153 147 159 133 165 152 148 161 129 164 153 179 150 146 162 134 185 146 159 139 137 168 159 SPILLED 157 158 155 125 181 SPILLED 134 149 137 152 129 163 148 152 152 141 156 150 172 135 138 159 131 168 SPILLED 148 135 142 159 155 143 161 165 161 NOT TAKEN 171 142 140 158 141 159 130 162 NOT TAKEN 157 152 134 NOT TAKEN 154 180 137 134 157 131 169 138 151 134 141 162 155 145 157 162 156 170 147 133 160 135 153 121 173 157 161 132 149 183 145 142 NOT TAKEN 134 135 NOT TAKEN 153 157 NOT TAKEN 121 126 NOT TAKEN 166 176 NOT TAKEN 138 140 NOT TAKEN 150 157 NOT TAKEN 128 136 NOT TAKEN 136 137 NOT TAKEN 154 154 NOT TAKEN 155 145 NOT TAKEN 145 140 NOT TAKEN 154 SPILLED NOT TAKEN 162 160 NOT TAKEN 163 156 NOT TAKEN 171 165 NOT TAKEN 145 147 NOT TAKEN 133 125 NOT TAKEN 154 154 NOT TAKEN 140 NOT TAKEN 162 164 NOT TAKEN 122 105 NOT TAKEN 169 153 NOT TAKEN 155 147 NOT TAKEN 160 155 NOT TAKEN 129 126 NOT TAKEN 149 142 NOT TAKEN 168 153 NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 11 WEEK WEEK 910 1317 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 5 - 300 PPM C91040 165 157 GROUP: MALE 6 - 100 PPM C91041 C91042 C91043 C91044 C91045 C91046 C91047 C91048 C91049 C91050 C91051 C91052 C91053 C91054 C91055 C91056 C91057 C91058 C91059 C91060 C91061 C91062 C91063 C91064 C91065 C91066 C91067 182 173 168 168 176 174 161 160 182 194 175 191 SPILLED 179 184 163 167 176 163 195 163 169 153 157 169 170 170 175 167 161 158 177 170 162 164 185 189 171 221 187 183 181 166 169 171 163 SPILLED 165 199 158 152 166 171 169 160 175 168 164 166 183 175 161 161 187 201 181 200 168 184 185 163 166 169 169 156 168 179 152 159 168 174 170 159 162 157 159 NOT TAKEN 178 180 182 180 184 165 164 164 157 157 171 170 176 173 181 177 178 176 173 175 194 189 193 183 177 173 182 129 NOT TAKEN 163 158 161 158 95 161 164 177 170 176 191 191 194 195 201 209 198 202 191 200 183 184 182 184 190 212 SPILLED 209 207 211 163 161 161 178 SPILLED 185 185 179 179 183 194 190 190 192 196 170 165 162 158 116 172 171 171 168 175 176 172 174 161 162 177 182 178 175 179 164 170 168 168 170 170 171 168 171 176 179 180 179 180 180 146 156 150 142 149 168 178 172 170 171 170 174 175 170 168 171 176 169 175 184 170 170 174 174 178 Covance 6329-212 3M T-6316.1 PAGE: 12 WEEK 9 WEEK 10 186 184 161 168 173 176 165 177 177 175 137 157 179 180 200 196 172 179 198 200 226 206 158 155 172 161 186 188 166 173 169 165 154 144 173 175 161 165 175 175 174 165 135 131 163 164 162 158 175 175 173 169 1318 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 6 - 100 PPM C91068 144 132 121 112 106 91 94 89 C91069 176 172 172 167 175 180 178 187 C91070 163 157 162 166 165 162 161 162 C91071 182 186 189 192 194 194 190 196 C91072 154 155 161 158 156 156 156 157 C91073 178 181 185 178 183 182 178 184 C91074 162 159 162 165 160 157 158 155 C91075 171 179 187 181 196 181 181 181 C91076 169 167 169 163 160 124 128 168 C91077 158 158 164 165 169 169 170 167 C91078 157 154 157 159 155 152 147 148 C91079 179 177 179 184 185 185 185 181 C91080 173 166 168 171 175 169 171 174 GROUP: MALE 7 - 300 PPM C91081 SPILLED 150 145 189 146 144 142 NOT TAKEN C91082 143 134 130 126 127 121 124 NOT TAKEN C91083 151 139 135 134 135 142 138 NOT TAKEN C91084 141 134 131 130 135 136 139 NOT TAKEN C91085 SPILLED 154 143 146 129 120 108 NOT TAKEN C91086 155 146 153 150 148 143 140 NOT TAKEN C91087 158 151 139 134 132 123 122 NOT TAKEN C91088 153 150 150 146 137 131 130 NOT TAKEN C91089 180 170 165 167 170 174 164 NOT TAKEN C91090 151 157 155 150 155 151 160 NOT TAKEN C91091 155 149 144 152 150 142 138 NOT TAKEN C91092 169 156 152 156 150 157 146 NOT TAKEN C91093 154 144 145 111 150 157 153 NOT TAKEN C91094 152 141 133 126 128 133 129 NOT TAKEN C91095 164 148 SPILLED 154 155 156 142 NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 13 WEEK 9 WEEK 10 33 39 180 176 160 160 193 190 150 148 174 183 152 149 171 174 166 165 168 165 141 142 170 169 172 170 1319 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 14 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 7 - 300 PPM C91096 C91097 C91098 C91099 C91100 C91101 C91102 C91103 C91104 C91105 C91106 C91107 C91108 C91109 C91110 C91111 C91112 C91113 C91114 C91115 C91116 C91117 C91118 C91119 C91120 136 134 129 128 134 130 126 NOT TAKEN 169 153 147 142 144 140 139 NOT TAKEN 166 158 156 154 154 148 149 NOT TAKEN 153 129 126 143 114 NOT TAKEN 162 153 143 143 139 132 135 NOT TAKEN 169 160 159 166 159 166 175 NOT TAKEN 156 191 153 144 154 156 146 NOT TAKEN 152 141 144 144 141 141 134 NOT TAKEN 132 135 128 122 127 124 121 NOT TAKEN 159 159 136 132 135 138 139 NOT TAKEN 153 146 142 126 149 142 152 NOT TAKEN 142 136 133 135 135 135 133 NOT TAKEN 165 159 156 154 149 152 151 NOT TAKEN 154 153 142 139 140 148 135 NOT TAKEN 166 155 160 151 155 155 144 NOT TAKEN 147 142 138 135 141 149 150 NOT TAKEN 176 170 168 166 168 165 165 NOT TAKEN 158 140 138 127 123 115 127 NOT TAKEN 187 170 169 156 163 162 155 NOT TAKEN 176 172 173 167 158 164 160 NOT TAKEN 155 144 141 133 142 138 165 NOT TAKEN 189 SPILLED SPILLED 193 SPILLED 178 SPILLED NOT TAKEN 158 148 149 146 149 162 139 NOT TAKEN 176 172 171 168 175 175 172 NOT TAKEN 179 164 166 163 168 132 27 NOT TAKEN GROUP: FEMALE 1 - 0 PPM C91121 118 SPILLED 143 143 139 141 150 144 137 145 C91122 SPILLED 136 SPILLED 162 146 148 169 157 SPILLED 134 C91123 108 122 121 136 127 126 129 114 114 119 1320 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 15 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C91124 C91125 C91126 C91127 C91128 C91129 C91130 C91131 C91132 C91133 C91134 C91135 C91136 C91137 C91138 C91139 C91140 C91141 C91142 C91143 C91144 C91145 C91146 C91147 C91148 C91149 C91150 C91151 C91152 C91153 C91154 FEMALE 1 - 0 PPM 128 102 189 117 134 130 131 SPILLED 183 127 134 115 136 134 106 174 140 127 129 104 122 130 116 125 120 104 115 131 120 134 134 129 109 SPILLED 127 160 147 128 123 SPILLED 130 137 117 143 115 102 240 156 115 145 112 115 140 125 118 118 123 120 125 134 146 146 137 112 SPILLED 137 150 156 136 SPILLED SPILLED SPILLED 142 SPILLED 140 109 SPILLED SPILLED 165 130 150 131 126 135 126 123 138 117 141 128 124 138 163 143 114 142 152 157 167 145 SPILLED SPILLED 138 140 NOT TAKEN 149 105 110 SPILLED SPILLED 134 159 NOT TAKEN 124 145 133 135 139 SPILLED 131 146 130 161 150 142 117 SPILLED 134 175 156 165 SPILLED SPILLED 148 137 143 111 107 193 186 137 158 115 145 138 132 129 159 119 168 146 148 149 139 124 137 134 155 152 SPILLED SPILLED SPILLED 142 142 152 115 111 146 176 141 151 117 138 135 160 130 SPILLED 124 149 137 142 133 139 113 SPILLED 136 159 156 147 SPILLED SPILLED 156 142 156 104 107 SPILLED 162 SPILLED 150 118 129 133 139 160 SPILLED 125 168 143 138 147 137 120 NOT TAKEN 131 153 150 146 158 SPILLED 140 145 142 106 107 SPILLED NOT TAKEN 136 140 114 NOT TAKEN 130 129 132 SPILLED 119 154 140 129 148 122 121 125 143 145 148 SPILLED SPILLED 125 132 149 100 113 136 133 136 111 131 125 131 113 120 149 130 131 138 132 113 128 143 156 140 SPILLED SPILLED 131 144 144 108 108 SPILLED 131 147 112 136 147 SPILLED SPILLED 122 143 129 125 127 1321 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 16 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: C91155 C91156 C91157 C91158 C91159 C91160 C91161 C91162 C91163 C91164 C91165 C91166 C91167 C91168 C91169 C91170 C91171 C91172 C91173 C91174 C91175 C91176 C91177 C91178 C91179 C91180 C91181 C91182 C91183 C91184 C91185 FEMALE 1 - 0 PPM SPILLED 119 124 121 112 122 108 124 130 135 147 139 SPILLED SPILLED 115 SPILLED 121 202 125 115 157 126 SPILLED 134 144 122 151 124 233 127 136 SPILLED 132 114 127 118 121 121 127 132 136 134 140 SPILLED SPILLED 130 170 118 SPILLED 128 121 158 147 SPILLED 133 134 133 167 139 SPILLED 139 137 SPILLED 134 127 130 108 131 118 134 141 132 142 147 146 SPILLED 136 179 130 SPILLED 132 116 164 146 115 140 160 136 163 144 SPILLED 156 140 SPILLED 134 125 126 116 147 137 127 136 134 NOT TAKEN 148 132 152 NOT TAKEN SPILLED NOT TAKEN 158 131 116 175 155 SPILLED 139 154 139 168 137 SPILLED 173 148 SPILLED 132 131 129 108 SPILLED 140 136 129 143 146 138 SPILLED SPILLED 150 122 113 163 153 SPILLED 143 152 142 165 151 SPILLED 162 138 SPILLED 151 127 121 116 SPILLED 143 142 139 120 145 126 SPILLED SPILLED 137 125 112 185 151 108 142 149 143 155 144 SPILLED 155 151 SPILLED 131 135 120 106 SPILLED 148 133 125 132 134 132 170 SPILLED SPILLED 136 118 176 163 112 144 160 145 151 146 SPILLED 148 148 SPILLED 94 123 116 SPILLED 132 131 NOT TAKEN NOT TAKEN 144 154 129 157 SPILLED 133 123 116 172 143 110 140 145 156 142 142 SPILLED 150 145 SPILLED 123 121 121 127 125 149 123 146 123 143 147 129 115 108 174 162 109 137 136 128 129 141 SPILLED 147 138 190 108 121 114 105 170 148 133 136 126 SPILLED SPILLED 139 118 119 185 SPILLED 108 136 139 139 133 140 SPILLED 146 143 1322 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C91186 C91187 C91188 C91189 C91190 GROUP: C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 C91210 C91211 C91212 C91213 FEMALE 1 - 0 PPM 121 127 140 143 103 107 88 91 116 110 FEMALE 2 - 3 PPM 119 109 SPILLED 111 138 107 127 125 139 125 104 127 129 121 111 SPILLED 123 117 112 179 125 107 116 126 114 SPILLED 116 149 118 136 123 148 123 SPILLED 142 138 123 117 120 128 124 113 151 136 111 129 136 146 SPILLED 99 133 125 114 246 127 151 125 137 138 141 141 121 143 144 128 125 125 123 124 120 159 131 119 131 139 156 144 109 SPILLED 138 157 130 106 SPILLED 131 138 SPILLED 129 154 115 160 NOT TAKEN SPILLED 175 146 153 150 132 NOT TAKEN 128 147 SPILLED 116 SPILLED 143 125 134 145 119 SPILLED 135 146 111 169 167 137 129 139 148 136 135 146 134 116 SPILLED SPILLED SPILLED 127 138 148 132 104 SPILLED 134 137 SPILLED 147 156 109 SPILLED SPILLED 128 166 133 138 149 135 146 143 125 SPILLED 195 122 127 141 145 136 106 SPILLED 157 125 SPILLED 146 164 115 SPILLED SPILLED 107 132 129 129 137 136 148 134 121 144 138 122 132 137 144 119 106 SPILLED 148 134 SPILLED 138 150 104 146 SPILLED 126 SPILLED 125 130 169 136 145 SPILLED 124 SPILLED 133 119 131 Covance 6329-212 3M T-6316.1 PAGE: 17 WEEK 9 WEEK 10 134 151 127 108 SPILLED 130 147 128 104 217 151 125 SPILLED 143 136 97 154 SPILLED 130 113 124 117 130 130 142 127 118 SPILLED 133 107 126 145 124 156 145 141 97 190 SPILLED 142 127 124 114 148 138 136 140 124 SPILLED 134 112 125 1323 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 C91241 C91242 C91243 C91244 FEMALE 2 - 3 PPM 138 111 127 104 112 135 117 125 139 96 141 121 105 SPILLED 129 122 130 129 112 113 137 135 121 143 110 118 144 123 SPILLED 134 105 142 113 SPILLED 111 150 130 130 135 147 96 149 118 112 116 130 134 138 140 119 114 159 SPILLED 121 149 113 117 127 127 174 155 113 144 114 SPILLED 117 SPILLED 127 134 136 148 108 117 141 120 SPILLED 126 128 141 142 111 112 163 193 132 135 110 136 143 125 SPILLED 163 118 137 110 SPILLED 130 SPILLED SPILLED 129 137 NOT TAKEN 114 SPILLED 106 111 SPILLED 160 124 NOT TAKEN SPILLED 125 129 164 SPILLED 128 NOT TAKEN 114 135 139 136 209 SPILLED 118 141 106 SPILLED 127 137 153 129 137 117 132 118 124 SPILLED 148 122 138 106 125 167 SPILLED 130 112 135 141 135 SPILLED 158 115 148 115 158 127 SPILLED SPILLED 134 161 112 125 126 131 122 142 118 155 110 130 168 SPILLED 161 117 140 141 SPILLED SPILLED SPILLED 121 142 109 SPILLED 138 141 SPILLED 119 SPILLED 110 134 133 134 137 139 110 133 113 125 152 SPILLED SPILLED 118 140 147 135 SPILLED 142 127 143 116 SPILLED 132 179 185 125 150 115 SPILLED 100 139 124 145 117 136 113 133 154 SPILLED 128 106 134 141 203 180 SPILLED 122 Covance 6329-212 3M T-6316.1 PAGE: 18 WEEK 9 WEEK 10 135 111 SPILLED 126 170 SPILLED 117 143 104 122 108 136 115 136 108 130 109 117 162 174 109 108 124 140 126 SPILLED 161 104 131 104 SPILLED 126 SPILLED SPILLED 118 132 106 124 125 131 116 133 103 132 104 112 150 SPILLED 111 107 128 130 SPILLED SPILLED 187 111 1324 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 2 - 3 PPM C91245 C91246 C91247 C91248 C91249 C91250 144 154 167 153 134 146 127 133 129 SPILLED 113 113 GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 C91269 C91270 C91271 C91272 109 122 115 118 111 134 105 121 158 133 106 SPILLED 125 112 127 133 102 117 128 123 136 140 109 128 125 121 116 115 112 135 SPILLED 137 105 105 123 119 124 140 103 SPILLED 138 117 SPILLED 145 166 SPILLED SPILLED 130 137 SPILLED 118 132 112 120 117 SPILLED 132 135 124 130 SPILLED 101 131 SPILLED 119 145 105 111 131 123 138 129 193 SPILLED 146 137 190 111 137 SPILLED 153 134 150 115 NOT TAKEN 135 129 124 140 136 SPILLED 123 133 131 127 112 131 126 152 158 109 121 142 123 SPILLED NOT TAKEN 142 127 132 124 140 109 135 127 138 120 100 127 125 190 158 106 118 148 125 207 163 SPILLED 166 131 SPILLED SPILLED 138 131 131 140 130 116 134 125 140 125 99 128 124 127 171 109 112 133 127 180 SPILLED SPILLED 174 141 153 110 134 114 126 127 126 105 123 126 132 126 96 119 118 133 147 102 105 138 122 SPILLED 157 SPILLED 197 136 159 175 155 133 131 134 130 115 128 137 113 146 118 129 123 127 144 108 105 SPILLED 127 SPILLED Covance 6329-212 3M T-6316.1 PAGE: 19 WEEK 9 WEEK 10 173 SPILLED SPILLED 120 141 105 SPILLED SPILLED SPILLED 129 146 155 149 139 118 122 115 121 131 129 125 112 100 SPILLED 128 125 130 130 128 137 136 106 96 89 132 123 121 112 117 SPILLED 147 137 100 94 106 105 143 136 121 120 144 SPILLED 1325 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 C91300 C91301 C91302 C91303 FEMALE 3 - 30 PPM SPILLED 110 125 103 107 125 158 126 113 134 122 131 113 100 157 121 SPILLED 122 131 119 136 127 118 101 173 131 SPILLED 165 115 SPILLED 127 146 117 138 106 111 SPILLED 184 130 130 135 126 138 112 99 106 122 123 119 122 126 176 130 149 SPILLED SPILLED 126 SPILLED SPILLED 112 SPILLED 135 SPILLED 112 133 114 115 SPILLED SPILLED 134 SPILLED 149 133 146 108 101 132 122 SPILLED 124 124 133 151 129 179 SPILLED SPILLED 123 112 186 123 SPILLED 138 178 SPILLED 139 115 106 141 162 132 SPILLED 155 130 149 NOT TAKEN 115 NOT TAKEN 119 124 133 NOT TAKEN 146 SPILLED 136 SPILLED 121 133 136 115 SPILLED 120 SPILLED 144 150 SPILLED 155 110 122 144 155 132 SPILLED 166 133 146 115 124 127 121 133 SPILLED 133 154 114 148 139 112 SPILLED 121 SPILLED 135 SPILLED SPILLED 139 114 124 SPILLED 168 126 SPILLED 168 143 144 SPILLED 114 122 121 127 SPILLED 123 SPILLED 119 151 131 120 198 125 SPILLED 134 SPILLED 118 143 121 118 133 164 130 SPILLED 162 131 129 138 109 117 134 125 SPILLED 124 133 113 158 124 110 169 120 138 134 147 SPILLED SPILLED 117 116 SPILLED 151 128 SPILLED 153 122 SPILLED 113 110 125 129 134 SPILLED SPILLED 217 125 134 128 110 228 129 SPILLED 127 Covance 6329-212 3M T-6316.1 PAGE: 20 WEEK 9 WEEK 10 SPILLED SPILLED SPILLED 115 107 115 150 134 SPILLED 158 131 135 115 108 109 126 122 124 120 140 SPILLED SPILLED 126 101 SPILLED 126 115 121 145 150 155 112 111 134 158 126 SPILLED 151 130 128 111 103 113 112 122 SPILLED 126 130 114 189 122 110 SPILLED 132 SPILLED 136 1326 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C91304 C91305 C91306 C91307 C91308 C91309 C91310 GROUP: C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 C91328 C91329 C91330 C91331 FEMALE 3 - 30 PPM 147 135 SPILLED 129 126 125 119 147 138 SPILLED 137 133 131 123 FEMALE 4 - 100 PPM 101 100 124 113 115 SPILLED 119 138 123 131 120 120 119 116 107 104 112 129 116 111 127 121 103 146 125 SPILLED SPILLED 122 152 125 SPILLED 133 127 113 116 103 93 109 125 118 110 119 144 137 148 128 142 138 118 SPILLED 97 SPILLED 125 119 132 124 146 117 SPILLED 133 SPILLED SPILLED 114 108 112 111 114 119 116 136 149 159 SPILLED 145 150 139 129 160 166 130 136 148 149 140 125 105 132 127 112 NOT TAKEN NOT TAKEN SPILLED 137 140 189 141 118 124 111 115 114 124 133 118 132 SPILLED 101 SPILLED SPILLED 112 112 131 127 143 132 SPILLED 117 103 109 134 116 116 107 135 SPILLED 154 SPILLED 142 162 148 128 143 98 SPILLED SPILLED 118 108 133 115 SPILLED 138 137 118 106 106 112 110 116 115 132 156 156 SPILLED 124 162 155 132 108 99 122 SPILLED 120 107 123 112 134 126 SPILLED 119 106 115 113 110 114 107 129 168 163 SPILLED 163 170 145 130 117 104 150 SPILLED 122 109 122 110 SPILLED 136 125 121 109 110 118 114 116 109 133 Covance 6329-212 3M T-6316.1 PAGE: 21 WEEK 9 WEEK 10 145 150 150 155 121 120 123 119 188 SPILLED 141 143 137 124 SPILLED 105 131 SPILLED 122 103 120 106 129 133 115 114 108 102 111 112 110 109 124 105 100 SPILLED SPILLED 124 94 120 110 125 123 114 107 99 99 112 109 108 108 132 1327 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 C91359 C91360 C91361 C91362 FEMALE 4 - 100 PPM SPILLED 109 123 112 SPILLED 122 154 118 137 124 129 126 117 SPILLED SPILLED SPILLED 123 SPILLED SPILLED 121 133 120 137 123 137 98 87 137 119 SPILLED 153 124 117 124 114 SPILLED 115 SPILLED 113 147 117 116 125 113 111 141 SPILLED 118 125 SPILLED 130 126 122 SPILLED 139 137 93 132 SPILLED 129 126 195 136 SPILLED 125 118 SPILLED 115 139 123 150 128 111 112 113 110 131 SPILLED 120 121 SPILLED 121 134 113 SPILLED 123 140 99 130 131 SPILLED 120 189 130 NOT TAKEN 121 111 SPILLED 132 135 128 SPILLED 130 114 119 118 NOT TAKEN 139 SPILLED 122 119 NOT TAKEN 127 135 121 SPILLED 137 168 97 135 128 113 123 SPILLED 131 117 109 SPILLED 124 104 115 156 124 124 121 114 134 117 112 127 125 131 121 SPILLED 128 SPILLED 95 133 122 103 118 SPILLED 119 121 118 SPILLED 127 110 122 147 124 115 127 107 131 117 113 117 143 141 110 SPILLED 122 135 92 123 124 110 119 SPILLED 113 117 113 SPILLED 119 111 110 136 119 123 118 115 135 114 111 113 132 133 120 99 123 134 93 124 137 105 111 150 144 116 115 SPILLED 127 102 123 134 121 123 115 110 133 118 100 122 132 125 112 106 131 SPILLED 93 128 SPILLED 108 97 SPILLED Covance 6329-212 3M T-6316.1 PAGE: 22 WEEK 9 WEEK 10 124 106 116 275 116 87 109 126 116 125 117 112 126 112 99 134 135 121 112 97 136 124 90 132 SPILLED 104 114 113 105 110 112 171 122 99 109 122 116 115 112 111 129 112 107 113 128 119 111 99 141 118 86 128 SPILLED 110 116 SPILLED 1328 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 23 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 4 - 100 PPM C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 136 117 SPILLED 124 152 135 114 126 110 147 120 SPILLED 127 116 115 117 113 135 140 125 140 110 146 160 113 130 117 122 117 SPILLED 121 111 117 116 166 133 GROUP: FEMALE 5 - 300 PPM C91381 C91382 C91383 C91384 C91385 C91386 C91387 C91388 C91389 C91390 SPILLED 91 103 169 SPILLED 109 98 118 104 107 96 95 98 107 109 SPILLED 81 111 74 97 143 115 SPILLED 122 143 SPILLED 113 119 119 152 107 131 120 110 121 113 121 139 95 75 99 94 96 SPILLED 75 105 75 103 149 118 SPILLED 127 130 161 114 119 123 136 116 SPILLED 132 109 131 118 130 135 107 86 102 114 108 SPILLED 77 108 82 107 127 111 SPILLED 118 135 143 125 119 131 145 112 130 124 115 125 123 134 135 101 81 99 SPILLED 107 SPILLED 101 102 76 103 138 108 114 129 142 119 122 115 125 119 111 125 119 112 119 119 127 129 101 86 101 106 145 SPILLED 79 98 81 110 132 96 109 122 122 118 116 109 SPILLED 119 109 109 111 108 122 118 115 129 97 78 88 SPILLED 120 SPILLED 79 103 87 108 139 102 133 128 142 114 116 115 SPILLED SPILLED 109 115 117 114 124 112 110 133 125 96 SPILLED 118 117 100 119 115 SPILLED 111 110 116 120 104 119 103 118 129 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 121 97 SPILLED 103 122 102 122 110 180 117 102 106 112 103 115 108 120 140 1329 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 5 - 3 0 0 PPM C91391 C91392 C91393 C91394 C91395 C91396 C91397 C91398 C91399 C91400 C91401 C91402 C91403 C91404 C91405 C91406 C91407 C91408 C91409 C91410 C91411 C91412 C91413 C91414 C91415 C91416 C91417 C91418 C91419 C91420 C91421 109 SPILLED 86 110 124 SPILLED 117 114 117 126 106 92 SPILLED 94 106 109 111 118 SPILLED SPILLED 120 115 SPILLED 121 107 111 122 107 SPILLED 112 91 102 111 143 106 118 SPILLED 92 94 101 113 125 84 155 88 98 99 93 116 84 118 115 97 SPILLED 114 90 114 SPILLED 104 SPILLED 135 90 97 SPILLED SPILLED 96 168 99 94 103 93 124 93 84 141 90 94 91 99 109 82 90 107 99 SPILLED 104 95 100 109 106 100 103 83 100 91 73 94 101 111 99 102 91 133 90 88 SPILLED 95 98 SPILLED 94 SPILLED 76 84 105 86 SPILLED 103 77 98 109 100 SPILLED 103 79 94 103 66 89 SPILLED 94 101 98 90 131 85 83 SPILLED 92 103 92 97 116 87 83 102 95 107 116 88 103 94 103 99 94 87 92 96 66 88 98 97 102 100 93 132 85 85 SPILLED 97 106 92 SPILLED 106 80 81 105 98 SPILLED 113 85 105 91 94 98 95 84 94 101 72 88 98 96 97 95 97 125 84 86 SPILLED 93 105 93 99 108 83 85 98 87 94 103 86 99 93 93 102 96 87 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 24 WEEK WEEK 910 1330 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 5 - 3 0 0 PPM C91422 C91423 C91424 C91425 C91426 C91427 C91428 C91429 C91430 C91431 C91432 C91433 C91434 C91435 C91436 C91437 C91438 C91439 C91440 C91441 C91442 C91443 C91444 C91445 C91446 C91447 C91448 C91449 C91450 SPILLED 102 103 SPILLED 107 125 118 131 104 107 95 167 109 115 84 106 111 133 SPILLED 112 113 110 113 SPILLED 107 121 110 117 102 88 111 100 94 137 SPILLED 108 115 101 91 105 153 102 112 92 105 105 113 116 99 100 95 98 100 96 102 SPILLED 130 95 88 113 93 87 83 114 113 102 100 87 92 SPILLED 95 112 88 101 100 103 105 88 101 102 100 SPILLED 89 97 SPILLED SPILLED 95 79 NOT TAKEN 90 80 63 NOT TAKEN 108 105 NOT TAKEN SPILLED 104 SPILLED 96 114 85 106 103 SPILLED 105 96 100 NOT TAKEN 102 86 90 92 NOT TAKEN SPILLED 87 73 91 84 80 109 111 SPILLED 106 SPILLED 93 111 78 105 104 100 98 96 96 100 87 97 98 179 70 77 94 81 100 103 103 SPILLED 98 85 86 110 93 105 100 100 98 93 102 98 SPILLED 95 93 SPILLED 76 79 91 85 81 107 98 SPILLED 103 95 87 113 91 111 100 88 106 94 93 94 89 100 126 SPILLED 72 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 25 WEEK WEEK 910 1331 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 6 - 100 PPM C91451 103 105 103 113 120 113 108 141 C91452 116 116 116 123 124 115 125 133 C91453 124 121 118 128 126 123 122 132 C91454 122 117 113 115 112 103 108 105 C91455 124 155 146 121 119 116 115 130 C91456 124 107 104 110 105 102 99 103 C91457 126 128 116 125 127 130 122 132 C91458 107 103 117 105 114 106 102 111 C91459 135 152 126 138 139 SPILLED 112 SPILLED C91460 99 106 104 103 101 96 85 90 C91461 108 116 119 121 122 121 110 123 C91462 124 122 131 131 132 131 133 136 C91463 SPILLED 123 112 111 115 102 110 103 C91464 107 107 111 114 116 109 105 108 C91465 120 SPILLED 126 116 124 130 108 128 C91466 125 119 135 142 127 109 123 122 C91467 123 145 108 125 115 135 120 SPILLED C91468 103 99 105 101 103 105 90 114 C91469 108 106 114 107 108 106 105 110 C91470 140 150 146 140 148 150 127 157 C91471 109 105 110 111 118 116 110 111 C91472 130 120 138 149 157 128 125 SPILLED C91473 111 103 107 107 111 106 118 SPILLED C91474 145 150 140 150 122 144 136 163 C91475 167 152 151 SPILLED 139 153 188 SPILLED C91476 SPILLED 121 116 124 123 120 120 120 C91477 103 103 106 SPILLED 112 107 117 114 C91478 SPILLED 159 151 135 130 123 123 SPILLED C91479 160 160 142 144 142 134 132 147 C91480 119 115 118 113 109 108 108 106 C91481 112 111 116 114 112 111 104 103 Covance 6329-212 3M T-6316.1 PAGE: 26 WEEK 9 WEEK 10 103 120 119 96 117 99 125 102 125 85 112 129 101 101 105 125 108 89 104 139 113 SPILLED 106 145 132 116 110 136 138 105 105 110 129 121 106 117 106 121 103 SPILLED 102 111 127 99 104 108 114 113 113 105 145 114 136 129 SPILLED SPILLED 120 120 128 124 110 113 1332 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C91482 C91483 C91484 C91485 C91486 C91487 C91488 C91489 C91490 GROUP: C91491 C91492 C91493 C91494 C91495 C91496 C91497 C91498 C91499 C91500 C91501 C91502 C91503 C91504 C91505 C91506 C91507 C91508 C91509 FEMALE 6 - 100 PPM 115 118 149 131 114 113 112 115 118 114 133 115 136 102 105 120 124 FEMALE 7 - 300 PPM 109 SPILLED SPILLED 106 115 113 115 99 107 115 105 125 134 SPILLED 108 118 123 SPILLED 115 110 166 103 94 101 100 107 121 115 106 86 110 129 SPILLED 97 112 114 145 114 127 158 115 115 118 111 133 114 121 99 103 SPILLED 87 92 90 102 87 89 94 96 111 110 SPILLED 96 104 106 116 94 130 SPILLED 114 113 122 110 SPILLED 121 SPILLED 103 SPILLED 93 91 92 91 103 95 86 92 87 108 112 SPILLED 93 101 153 119 SPILLED 125 149 118 112 116 111 155 111 124 96 106 91 87 94 93 103 92 80 82 90 105 114 SPILLED 96 107 106 SPILLED SPILLED 122 114 115 114 110 104 126 110 115 93 101 85 83 86 86 101 118 78 83 SPILLED 100 104 164 SPILLED 103 103 SPILLED 95 119 131 104 104 111 104 122 108 116 90 97 89 89 97 90 103 SPILLED 80 80 84 105 115 121 91 102 98 SPILLED 89 120 SPILLED 116 117 108 113 123 114 118 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 27 WEEK 9 WEEK 10 119 115 116 106 110 105 105 106 110 106 104 103 123 120 118 109 123 112 1333 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 7 - 3 0 0 PPM C91510 115 114 105 108 100 99 106 NOT TAKEN C91511 114 99 SPILLED 98 101 101 100 NOT TAKEN C91512 102 104 94 91 93 90 90 NOT TAKEN C91513 109 111 95 95 92 88 84 NOT TAKEN C91514 127 120 116 111 109 105 108 NOT TAKEN C91515 SPILLED SPILLED 109 94 94 108 99 NOT TAKEN C91516 109 97 91 98 93 96 90 NOT TAKEN C91517 98 SPILLED 81 81 88 83 83 NOT TAKEN C91518 113 114 94 100 100 98 107 NOT TAKEN C91519 105 SPILLED SPILLED SPILLED SPILLED SPILLED 171 NOT TAKEN C91520 105 96 95 98 97 95 104 NOT TAKEN C91521 113 102 97 88 101 95 84 NOT TAKEN C91522 150 133 108 104 124 97 94 NOT TAKEN C91523 111 137 139 101 112 105 105 NOT TAKEN C91524 SPILLED 107 102 98 113 105 111 NOT TAKEN C91525 169 108 102 100 98 97 97 NOT TAKEN C91526 117 105 102 104 101 100 102 NOT TAKEN C91527 127 SPILLED 111 104 SPILLED 112 109 NOT TAKEN C91528 101 85 84 81 74 84 75 NOT TAKEN C91529 SPILLED 131 132 123 107 111 104 NOT TAKEN C91530 110 101 91 100 89 92 94 NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 28 WEEK WEEK 910 1334 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C90711 C90712 C90713 C90714 C90715 C90716 C90720 C90721 C90722 C90723 C90724 C90726 C90727 C90728 C90729 C90730 C90731 C90733 C90734 C90735 C90736 C90738 C90739 C90740 C90741 C90742 C90743 C90744 C90745 C90746 C90747 C90748 MALE 1 - 0 PPM 178 175 184 172 200 198 185 186 172 172 158 155 187 182 189 199 163 161 SPILLED SPILLED 174 164 203 SPILLED 204 189 173 174 174 171 184 182 195 203 125 133 212 SPILLED 166 169 184 172 185 187 177 183 179 179 176 178 166 159 189 186 202 203 162 195 200 159 165 169 191 189 167 125 210 188 182 157 179 258 163 SPILLED 168 SPILLED 192 183 178 188 204 192 211 173 191 193 183 182 175 168 185 203 181 243 168 185 178 179 203 187 179 SPILLED 182 267 164 195 169 186 195 183 182 188 NOT TAKEN 193 214 175 187 175 187 179 183 170 195 205 166 NOT TAKEN 177 NOT TAKEN 178 SPILLED 211 179 193 159 187 257 167 199 177 190 195 182 190 193 193 220 177 190 194 189 186 182 177 197 207 191 177 174 SPILLED 207 176 187 163 182 SPILLED 169 196 173 190 189 183 189 191 198 223 174 187 192 184 187 182 174 191 204 185 180 191 188 209 191 191 162 192 246 169 209 173 186 194 187 185 195 205 226 180 189 195 190 198 181 177 205 213 203 190 181 186 223 183 182 161 189 247 167 202 173 185 191 189 181 193 214 224 178 184 179 183 195 189 166 204 203 197 170 Covance 6329-212 3M T-6316.1 PAGE: 29 WEEK 28 184 177 217 183 194 160 192 245 181 203 172 175 192 187 180 202 197 236 187 189 193 180 175 171 168 187 198 197 183 WEEK 32 188 164 211 180 190 162 193 243 178 215 175 181 193 191 188 191 194 226 184 202 188 180 186 180 182 209 207 191 215 1335 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 1 - 0 PPM C90749 168 165 162 167 168 165 168 171 C90750 161 157 169 163 164 168 167 168 C90751 201 SPILLED 209 SPILLED 215 SPILLED 219 207 C90752 207 208 206 203 204 203 195 192 C90754 207 198 192 203 199 195 200 195 C90755 213 197 209 213 219 214 228 219 C90757 188 183 185 188 190 186 192 196 C90758 216 236 232 SPILLED 255 SPILLED SPILLED 241 C90759 174 170 173 170 174 171 165 159 C90760 202 199 194 165 202 204 190 188 C90761 181 173 174 172 177 183 175 178 C90762 169 169 179 175 173 172 169 173 C90763 196 196 192 199 195 190 199 194 C90764 184 172 173 173 174 168 172 162 C90765 202 192 199 199 205 205 202 198 C90766 178 170 179 155 175 180 188 176 C90767 183 180 189 195 203 188 193 197 C90768 174 176 178 NOT TAKEN C90770 SPILLED 185 190 182 184 187 186 188 C90771 174 163 179 179 175 191 189 176 C90772 188 187 187 180 182 183 187 187 C90773 188 184 190 182 182 190 193 187 C90774 176 175 180 175 179 177 177 177 C90776 198 200 207 205 206 204 209 203 C90777 206 209 210 206 212 208 217 203 C90778 173 173 176 169 171 172 182 181 C90779 180 182 183 182 190 190 191 194 C90780 206 210 210 NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 30 WEEK 28 WEEK 32 171 169 225 192 195 218 197 SPILLED 162 189 173 159 192 168 203 177 196 178 178 188 191 188 204 211 182 197 188 172 219 204 210 238 195 203 175 194 175 160 181 178 208 206 190 174 186 190 203 174 213 208 187 194 1336 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 2 - 3 PPM C90781 187 187 188 190 188 177 182 183 C90782 211 180 185 183 183 184 179 181 C90783 170 154 158 157 165 164 164 167 C90784 176 169 181 181 173 176 186 176 C90785 184 189 197 198 201 192 201 200 C90786 163 165 173 175 175 158 176 176 C90787 197 197 190 190 193 190 205 186 C90788 180 180 182 178 184 189 189 186 C90790 207 208 204 196 205 201 223 205 C90791 169 189 168 149 179 173 181 177 C90792 184 181 187 187 160 143 199 154 C90793 174 174 171 168 180 178 172 169 C90794 177 186 200 198 201 215 211 200 C90795 187 198 193 184 185 179 181 177 C90796 204 SPILLED 214 211 198 192 191 195 C90797 173 172 174 NOT TAKEN C90798 176 173 175 179 176 177 197 189 C90799 163 165 166 165 167 173 181 170 C90800 183 182 194 181 188 188 192 190 C90801 190 190 198 194 200 189 197 199 C90802 148 149 157 146 157 161 162 167 C90803 189 191 191 193 202 198 198 194 C90804 205 200 204 199 210 196 202 190 C90805 186 181 195 198 200 204 205 205 C90806 158 164 159 159 165 167 172 172 C90807 170 175 180 NOT TAKEN C90808 200 160 SPILLED 202 200 201 204 206 C90809 174 181 183 179 177 183 190 182 C90810 197 190 199 196 195 197 197 205 C90811 195 192 190 193 190 193 193 196 C90812 173 171 178 178 179 185 188 179 Covance 6329-212 3M T-6316.1 PAGE: 31 WEEK 28 WEEK 32 177 182 108 186 163 170 178 183 194 198 175 187 185 191 193 195 198 222 167 184 145 191 170 177 106 28 170 186 194 204 189 188 154 176 185 193 173 194 165 182 195 197 204 205 195 203 181 186 194 216 177 180 174 195 186 182 185 197 1337 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 2 - 3 PPM C90815 187 187 183 181 187 181 189 182 C90816 198 197 197 181 200 198 201 201 C90818 205 196 212 NOT TAKEN C90819 180 172 165 167 183 191 181 190 C90820 172 170 180 148 176 175 176 171 C90821 198 198 199 200 203 205 214 203 C90822 162 153 162 167 171 172 174 173 C90823 178 173 183 185 186 188 190 180 C90824 176 166 171 180 178 177 182 180 C90825 194 183 185 189 196 186 194 186 C90826 234 222 SPILLED SPILLED 250 244 248 240 C90827 191 182 183 179 181 186 201 193 C90828 204 205 219 224 210 221 215 216 C90829 196 185 196 204 203 208 206 219 C90830 195 184 183 185 198 190 193 190 C90831 199 196 205 NOT TAKEN C90832 191 182 186 182 188 181 189 184 C90835 193 180 173 173 179 185 185 182 C90836 185 185 190 NOT TAKEN C90837 166 160 168 169 162 163 168 164 C90838 217 219 221 223 236 228 237 226 C90839 212 211 212 214 208 217 233 220 C90840 192 191 188 168 203 199 205 205 GROUP: MALE 3 - 30 PPM C90841 182 177 174 177 174 177 180 190 C90842 145 142 145 143 142 138 150 147 C90843 187 176 176 NOT TAKEN C90844 SPILLED 205 205 214 214 230 220 217 C90845 180 171 171 178 176 173 186 187 Covance 6329-212 3M T-6316.1 PAGE: 32 WEEK 28 WEEK 32 171 183 197 211 189 198 177 179 207 220 174 190 186 179 181 180 185 202 160 SPILLED 192 206 214 210 206 219 101 197 185 188 177 184 161 163 136 235 152 219 190 195 185 191 146 160 107 224 177 176 1338 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 3 - 30 PPM C90847 C90848 C90849 C90850 C90851 C90852 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90867 C90868 C90869 C90870 C90871 C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 171 186 194 161 185 178 189 182 181 178 157 194 151 182 152 199 162 149 158 198 180 182 153 164 190 190 168 205 170 168 182 164 193 189 156 167 183 183 186 169 179 149 193 165 180 151 192 162 149 157 197 171 180 155 164 177 196 169 193 170 173 176 167 160 167 173 182 181 184 191 196 196 198 192 189 185 183 190 188 191 157 155 154 154 159 159 170 149 172 166 179 171 182 NOT TAKEN 191 SPILLED SPILLED 199 SPILLED 212 198 187 187 189 191 185 174 145 168 169 191 184 184 176 184 187 193 186 152 159 157 163 165 170 203 194 190 189 204 198 167 162 169 164 173 157 186 174 182 189 191 187 156 152 155 158 169 164 200 NOT TAKEN 170 163 169 168 181 173 153 151 155 154 162 163 164 160 159 159 163 166 197 196 200 204 207 194 178 168 171 177 181 174 186 178 192 165 191 190 157 152 161 160 162 158 170 175 170 176 180 181 191 197 207 188 205 194 194 184 189 192 189 189 180 173 187 182 185 183 195 184 178 175 201 206 172 NOT TAKEN 176 174 172 176 184 180 179 178 178 183 191 205 Covance 6329-212 3M T-6316.1 PAGE: 33 WEEK 28 WEEK 32 173 205 184 159 167 SPILLED 168 170 177 168 181 157 186 157 163 160 173 191 186 190 150 190 204 191 170 196 177 196 191 198 201 163 177 226 188 190 183 174 214 166 191 168 178 173 174 201 193 199 164 175 198 193 182 194 169 195 1339 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 3 - 30 PPM C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 202 185 181 SPILLED 186 170 199 186 167 157 149 187 178 195 SPILLED 190 174 157 174 194 185 172 217 179 165 189 175 168 149 140 172 167 183 187 182 165 147 170 GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 149 151 145 160 160 174 154 166 191 151 153 142 150 160 173 147 171 191 205 192 179 191 SPILLED 167 192 179 171 146 143 172 170 198 198 190 162 162 175 NOT TAKEN 188 180 SPILLED 177 169 190 185 82 148 127 175 182 182 178 144 168 150 171 182 178 210 SPILLED 170 188 SPILLED 209 150 147 175 176 183 SPILLED 191 162 160 177 191 183 207 SPILLED 173 194 196 190 160 142 180 180 177 178 202 164 158 185 191 179 201 SPILLED 191 209 197 181 162 147 187 184 179 195 208 183 160 187 151 157 163 159 172 160 158 160 161 168 146 142 147 154 152 159 157 156 154 160 163 NOT TAKEN 172 175 178 177 187 155 NOT TAKEN 177 175 183 178 184 192 194 192 194 218 199 186 210 200 182 198 201 169 161 142 178 185 176 186 201 179 170 180 166 164 155 167 189 185 216 Covance 6329-212 3M T-6316.1 PAGE: 34 WEEK 28 WEEK 32 195 191 184 187 205 186 192 186 176 185 194 214 193 215 171 185 160 177 145 151 177 196 178 193 175 189 176 190 190 203 167 177 157 173 179 187 170 177 168 172 151 148 156 159 195 204 190 179 199 198 1340 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 4 - 100 PPM C90910 170 160 170 167 173 172 178 178 C90911 175 165 175 175 176 175 183 176 C90912 170 168 175 171 169 171 190 176 C90913 177 162 158 176 170 178 184 179 C90914 SPILLED SPILLED 195 189 188 188 200 197 C90915 185 183 181 190 193 191 186 194 C90916 149 146 150 154 158 156 162 166 C90917 168 169 170 NOT TAKEN C90918 178 180 183 181 181 173 188 176 C90919 175 175 171 173 171 177 183 182 C90920 162 161 167 163 160 168 181 171 C90921 171 171 168 NOT TAKEN C90922 155 155 163 164 162 166 179 174 C90923 171 166 167 171 173 170 182 173 C90924 190 186 187 187 176 181 199 179 C90925 157 156 157 160 163 165 169 164 C90926 172 176 170 177 179 187 193 188 C90927 162 161 166 165 167 169 173 178 C90928 147 146 154 153 154 162 162 162 C90929 148 143 105 152 162 160 161 160 C90930 178 183 180 178 180 177 170 174 C90931 166 160 168 168 168 169 178 175 C90932 149 152 156 159 162 152 163 173 C90933 173 175 173 183 186 182 194 189 C90934 162 157 158 145 171 166 162 166 C90936 166 169 169 170 171 170 119 184 C90937 156 153 154 159 154 131 181 175 C90938 163 157 159 153 163 166 173 180 C90940 192 188 189 187 188 186 202 203 C90941 178 168 176 169 173 180 193 204 C90943 180 197 SPILLED 220 SPILLED 188 SPILLED 192 Covance 6329-212 3M T-6316.1 PAGE: 35 WEEK 28 WEEK 32 170 175 173 175 160 188 182 176 192 197 206 202 170 175 182 193 181 170 173 173 172 177 181 191 175 158 176 173 187 202 171 211 157 161 167 173 178 176 173 181 169 178 182 182 165 181 173 182 188 180 183 190 208 200 191 191 188 SPILLED 1341 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 4 - 100 PPM C90944 C90945 C90946 C90947 C90948 C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90962 C90963 C90964 C90965 C90967 C90968 C90969 C90970 141 149 140 161 162 75 171 169 180 159 154 166 145 168 161 142 165 185 171 186 143 166 162 206 160 143 142 138 147 146 152 153 152 148 153 154 153 156 159 154 166 161 165 164 180 174 158 161 153 165 172 177 175 166 170 172 175 175 184 188 55 NOT TAKEN 168 174 167 174 180 182 179 168 168 173 165 173 174 167 171 173 170 169 186 192 183 160 159 140 164 162 160 156 150 153 165 160 159 164 165 157 161 163 171 168 172 178 148 146 162 163 164 178 167 149 160 150 168 167 103 152 128 179 176 176 187 189 SPILLED 155 160 160 170 171 181 170 163 173 NOT TAKEN 179 179 177 181 181 181 187 173 176 183 181 179 189 182 188 184 184 192 197 209 206 144 80 152 SPILLED 161 SPILLED 175 161 168 167 169 165 168 166 164 166 151 169 171 177 173 204 209 209 201 204 215 212 153 158 158 163 162 170 161 GROUP: MALE 6 - 100 PPM C91041 188 181 184 180 186 187 186 185 C91042 165 171 177 165 167 176 181 173 C91043 180 174 181 175 172 177 180 188 Covance 6329-212 3M T-6316.1 PAGE: 36 WEEK 28 WEEK 32 155 158 162 170 164 153 169 181 181 173 180 190 172 175 189 202 158 169 166 183 177 180 172 164 161 176 179 184 176 177 182 193 187 176 202 199 149 SPILLED 172 180 165 181 228 222 161 156 183 186 185 192 185 184 1342 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 6 - 100 PPM C91044 184 179 181 177 180 180 187 194 C91045 181 186 184 182 176 185 189 190 C91047 157 162 156 165 161 154 160 164 C91048 179 176 186 172 177 188 189 198 C91049 197 189 191 189 199 202 214 222 C91050 192 191 191 189 193 197 208 192 C91051 194 191 195 192 192 190 206 205 C91052 203 200 SPILLED 193 207 204 219 211 C91053 159 161 166 168 169 170 174 176 C91054 163 161 169 162 167 187 189 181 C91055 189 183 186 193 201 192 203 197 C91056 183 188 188 199 196 195 187 197 C91057 166 170 165 168 172 179 171 174 C91058 143 141 149 153 157 153 154 158 C91059 175 172 172 173 171 164 172 173 C91060 167 157 164 162 170 168 176 165 C91061 174 180 188 188 181 184 186 187 C91062 172 168 173 174 175 181 176 180 C91063 131 143 143 140 141 140 146 135 C91064 162 165 168 176 178 180 178 183 C91065 158 158 169 164 168 168 157 167 C91066 171 172 177 178 176 171 181 182 C91067 173 171 174 170 172 175 176 181 C91068 53 NOT TAKEN C91069 191 185 178 175 179 180 194 182 C91070 156 151 150 156 165 162 161 170 C91071 196 190 199 193 198 191 192 194 C91072 150 145 153 148 149 151 147 150 C91073 195 191 194 199 192 201 186 196 C91074 151 148 157 150 149 153 163 156 C91075 178 174 181 169 176 175 188 192 Covance 6329-212 3M T-6316.1 PAGE: 37 WEEK 28 WEEK 32 194 190 158 187 204 191 200 205 171 169 199 SPILLED 167 154 178 162 172 186 141 181 164 176 184 193 161 195 149 189 157 189 198 184 138 183 213 206 206 214 169 188 199 SPILLED 176 167 171 165 175 SPILLED 152 178 151 182 196 202 178 199 150 182 167 SPILLED 1343 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 6 - 100 PPM C91076 C91077 C91078 C91079 C91080 173 167 145 172 170 175 164 145 168 169 GROUP: FEMALE 1 - 0 PPM C91121 C91122 C91123 C91124 C91125 C91127 C91128 C91129 C91130 C91131 C91132 C91133 C91134 C91136 C91137 C91138 C91139 C91141 C91142 C91144 C91146 C91147 C91148 145 145 117 133 104 123 152 136 122 SPILLED SPILLED 146 138 143 95 111 SPILLED 145 139 113 124 136 135 139 122 116 120 106 121 146 147 125 138 SPILLED 134 120 135 91 111 141 129 115 108 125 149 126 168 169 148 170 176 137 106 108 117 107 115 135 151 132 182 SPILLED 126 135 137 91 113 138 116 127 101 121 163 112 171 172 173 177 148 143 172 167 170 168 144 106 113 112 113 114 149 NOT TAKEN 125 172 171 132 144 143 101 109 162 128 128 102 126 152 103 151 140 112 126 109 123 144 124 SPILLED SPILLED 130 134 151 98 114 134 140 128 107 124 SPILLED 119 167 177 156 174 168 144 128 114 125 106 119 145 126 SPILLED SPILLED 127 139 131 91 112 SPILLED 123 126 102 121 150 124 192 176 163 182 181 149 113 131 129 119 121 148 129 135 SPILLED 135 146 161 94 SPILLED SPILLED 126 142 106 137 SPILLED 129 176 161 152 172 180 136 147 118 120 116 124 161 105 113 SPILLED 140 149 150 101 145 125 126 150 108 132 145 106 Covance 6329-212 3M T-6316.1 PAGE: 38 WEEK 28 WEEK 32 180 177 150 178 173 157 135 130 125 111 136 154 124 128 185 124 151 165 101 SPILLED 126 123 137 106 138 SPILLED 127 183 188 163 178 175 150 129 127 133 117 129 169 115 110 190 135 120 155 100 155 153 124 152 113 146 182 140 1344 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C91149 C91150 C91151 C91152 C91153 C91154 C91155 C91156 C91157 C91158 C91159 C91160 C91161 C91164 C91166 C91167 C91168 C91170 C91172 C91173 C91174 C91175 C91176 C91177 C91178 C91179 C91180 C91181 C91182 C91183 C91184 FEMALE 1 - 0 PPM SPILLED 126 152 SPILLED 128 141 126 131 122 107 105 124 132 122 130 126 SPILLED SPILLED 118 119 117 174 146 105 135 136 123 133 137 SPILLED 145 SPILLED 119 147 133 134 130 132 141 128 107 111 132 129 128 122 127 SPILLED SPILLED 122 119 119 164 SPILLED 105 144 127 132 131 141 SPILLED 137 130 116 141 122 118 117 119 128 108 108 111 130 131 122 117 119 SPILLED SPILLED 116 114 109 163 126 102 120 120 133 130 134 SPILLED 117 135 123 139 136 134 130 NOT TAKEN 128 110 112 107 NOT TAKEN 133 113 120 119 138 SPILLED 114 115 NOT TAKEN 171 123 108 117 130 144 NOT TAKEN 112 SPILLED 134 SPILLED 130 134 126 117 131 139 125 111 101 117 127 120 113 SPILLED SPILLED 122 115 163 136 120 140 128 146 133 SPILLED 129 SPILLED 123 148 131 121 131 145 121 124 103 139 120 126 121 127 SPILLED 121 121 163 132 118 136 130 151 137 SPILLED 138 SPILLED 123 157 109 138 137 147 135 134 112 133 134 143 141 130 154 SPILLED 122 165 147 110 144 142 160 142 SPILLED 138 149 133 158 SPILLED 159 127 150 129 122 107 132 147 127 144 119 155 146 116 156 137 113 120 139 171 145 153 131 Covance 6329-212 3M T-6316.1 PAGE: 39 WEEK 28 WEEK 32 SPILLED 129 167 121 159 154 168 120 125 102 127 176 131 152 121 139 156 139 162 135 114 152 153 162 151 SPILLED 134 136 127 181 127 172 165 156 140 120 120 122 155 128 166 120 138 170 146 167 136 114 146 154 160 137 SPILLED 124 1345 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 1 - 0 PPM C91185 C91186 C91187 C91188 C91189 C91190 146 131 138 115 103 211 138 120 139 120 101 125 GROUP: FEMALE 2 - 3 PPM C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91199 C91200 C91201 C91202 C91203 C91204 C91206 C91207 C91208 C91209 C91210 C91211 C91212 C91213 C91214 120 122 SPILLED 137 136 104 150 SPILLED 178 SPILLED 124 119 141 135 133 119 106 SPILLED 115 119 130 130 125 115 SPILLED 132 127 108 141 150 124 135 113 116 138 121 124 123 104 SPILLED 124 116 114 106 128 120 129 115 106 127 112 116 SPILLED 131 129 99 SPILLED SPILLED 128 153 115 120 132 122 132 111 109 208 118 121 119 114 124 122 130 122 104 SPILLED 138 121 129 119 105 133 131 NOT TAKEN 128 137 128 94 144 201 118 134 115 129 113 135 123 124 122 179 121 117 132 121 128 124 127 131 106 SPILLED 234 118 120 128 134 138 137 128 SPILLED 111 SPILLED 121 112 132 134 130 122 133 114 107 126 112 SPILLED 133 120 107 141 157 SPILLED 119 126 134 137 131 127 124 118 SPILLED 124 115 125 133 139 129 136 117 108 147 154 162 134 149 110 143 SPILLED 127 151 114 129 138 152 127 SPILLED 107 SPILLED 132 128 128 120 141 125 138 124 106 152 146 147 147 155 122 158 126 117 132 130 136 128 173 130 134 123 SPILLED 130 128 139 151 Covance 6329-212 3M T-6316.1 PAGE: 40 WEEK 28 WEEK 32 144 121 142 123 111 137 149 SPILLED 148 155 119 148 130 124 146 137 128 119 166 139 141 130 179 152 128 106 128 131 128 141 136 112 159 149 125 148 154 133 161 143 120 135 134 138 143 163 141 134 144 181 124 133 141 125 1346 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91231 C91232 C91233 C91234 C91235 C91236 C91238 C91239 C91240 C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 FEMALE 2 - 3 PPM 102 162 123 SPILLED SPILLED 120 128 100 132 112 128 121 127 103 124 114 119 145 SPILLED 112 SPILLED 125 100 SPILLED 216 168 110 151 SPILLED SPILLED 131 109 SPILLED 121 SPILLED SPILLED 115 145 113 115 108 126 119 125 107 118 122 119 144 SPILLED 112 158 122 92 126 SPILLED SPILLED 105 SPILLED SPILLED SPILLED SPILLED 107 126 114 SPILLED SPILLED 108 139 111 112 109 136 112 132 115 121 113 111 151 SPILLED 107 104 124 122 129 SPILLED 150 104 144 SPILLED 207 115 102 128 113 SPILLED SPILLED 110 117 NOT TAKEN 114 113 128 111 106 106 124 106 NOT TAKEN 140 SPILLED 110 104 123 129 NOT TAKEN SPILLED 191 107 146 SPILLED 180 NOT TAKEN 111 138 119 SPILLED SPILLED 119 140 112 104 135 118 124 105 129 SPILLED 146 SPILLED 108 105 129 115 SPILLED SPILLED 108 SPILLED SPILLED SPILLED 120 125 114 SPILLED SPILLED 115 138 118 115 137 111 132 108 149 119 150 SPILLED 113 113 115 120 SPILLED 164 111 147 SPILLED 178 118 121 128 SPILLED SPILLED 115 160 115 102 147 110 121 125 125 130 150 183 123 105 139 117 SPILLED SPILLED SPILLED 141 SPILLED 182 129 131 118 142 SPILLED 134 143 123 137 148 109 139 115 120 121 146 155 147 121 130 153 224 188 127 163 222 SPILLED Covance 6329-212 3M T-6316.1 PAGE: 41 WEEK 28 WEEK 32 131 138 130 141 177 122 129 126 108 148 118 135 117 133 114 140 159 139 115 137 118 SPILLED 194 130 136 SPILLED SPILLED 148 137 132 SPILLED 140 136 158 138 126 154 122 133 116 142 116 181 143 154 137 144 122 221 174 135 139 SPILLED 189 1347 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 2 - 3 PPM C91249 145 135 131 124 135 136 146 141 C91250 107 100 138 100 103 106 105 102 GROUP: FEMALE 3 - 30 PPM C91252 137 126 123 139 133 134 145 129 C91253 119 123 125 114 124 118 133 121 C91254 119 108 111 106 113 111 107 104 C91255 130 126 128 117 118 126 120 126 C91256 123 122 118 114 116 117 129 121 C91257 SPILLED SPILLED 156 SPILLED 144 131 97 SPILLED C91258 124 116 114 112 123 110 129 119 C91259 SPILLED 122 117 121 124 127 134 135 C91260 136 121 119 130 143 134 144 147 C91261 118 125 122 121 122 133 136 141 C91262 92 97 94 88 104 97 115 111 C91263 130 127 128 131 137 140 152 150 C91264 102 116 106 104 124 112 128 111 C91265 111 129 106 110 116 113 110 112 C91266 141 137 123 131 130 125 131 172 C91267 105 98 97 103 100 105 105 121 C91268 109 107 100 94 105 102 104 118 C91269 142 148 147 130 135 SPILLED 136 122 C91270 139 113 109 124 122 127 131 127 C91271 195 145 141 142 140 135 141 145 C91273 153 156 157 156 SPILLED SPILLED 149 141 C91274 118 115 112 125 SPILLED 125 117 127 C91275 SPILLED 133 122 149 135 133 134 154 C91276 122 114 101 110 118 113 120 118 C91277 118 111 113 118 114 114 110 117 C91278 SPILLED 118 115 121 120 118 132 133 Covance 6329-212 3M T-6316.1 PAGE: 42 WEEK 28 WEEK 32 149 145 101 105 149 132 117 136 119 SPILLED 132 146 140 133 111 156 118 116 151 SPILLED 115 151 121 127 136 124 141 129 122 132 148 131 117 141 118 SPILLED 126 142 162 137 103 160 126 126 164 122 124 145 132 138 142 131 146 136 128 135 1348 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C91279 C91280 C91281 C91282 C91283 C91284 C91286 C91288 C91289 C91290 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 FEMALE 3 - 30 PPM 159 115 SPILLED 139 122 127 113 105 120 131 122 179 112 129 SPILLED 140 112 102 228 115 179 123 144 148 116 118 177 123 120 163 130 SPILLED 145 114 SPILLED 119 109 109 131 120 137 117 129 165 154 138 134 137 120 SPILLED 137 151 149 126 118 168 129 128 147 126 172 140 111 139 115 107 103 122 114 133 117 115 122 134 141 100 144 119 SPILLED 118 148 146 122 109 208 134 125 165 126 NOT TAKEN 150 115 SPILLED 109 NOT TAKEN 112 148 120 NOT TAKEN 116 130 123 SPILLED SPILLED NOT TAKEN 138 115 121 133 NOT TAKEN 134 117 121 SPILLED 136 122 162 134 144 105 134 SPILLED 111 158 130 119 SPILLED SPILLED 170 SPILLED SPILLED 120 114 141 157 127 113 SPILLED 144 124 165 126 147 114 129 SPILLED 109 162 129 115 125 SPILLED 144 SPILLED SPILLED 118 120 131 159 117 114 208 141 125 208 135 148 124 153 SPILLED 119 178 135 121 130 124 162 SPILLED SPILLED 127 124 142 170 124 127 179 147 124 174 138 143 129 145 SPILLED 122 192 120 118 115 114 171 SPILLED 166 137 137 131 163 124 133 175 148 130 Covance 6329-212 3M T-6316.1 PAGE: 43 WEEK 28 WEEK 32 177 134 158 130 141 SPILLED 130 139 129 127 136 121 SPILLED 117 152 118 135 125 167 121 122 SPILLED 145 123 189 133 152 122 126 142 126 211 120 152 129 120 196 SPILLED 152 121 145 158 180 149 138 159 156 122 1349 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C91311 C91312 C91313 C91314 C91315 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 C91328 C91329 C91330 C91331 C91332 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 FEMALE 4 - 100 PPM 97 101 144 145 124 94 123 111 125 125 116 112 102 104 110 108 105 108 127 120 108 107 SPILLED 115 92 106 117 112 110 117 107 107 100 115 SPILLED 113 97 117 113 127 125 120 151 101 102 98 113 102 104 112 121 114 103 SPILLED 120 85 113 112 108 112 116 103 124 101 117 SPILLED 113 90 117 109 113 124 122 99 100 92 106 97 101 104 116 110 109 104 162 113 91 103 105 105 103 116 100 105 103 117 SPILLED 109 95 116 118 128 122 129 107 108 89 106 109 NOT TAKEN 106 122 93 114 110 SPILLED NOT TAKEN 91 108 108 110 107 116 113 105 105 123 SPILLED 122 NOT TAKEN 122 116 129 128 129 106 106 109 107 112 116 126 SPILLED 109 104 SPILLED 97 114 107 112 106 120 109 113 104 118 SPILLED 117 121 110 119 138 129 108 110 97 110 104 110 125 108 109 109 SPILLED 100 110 114 109 115 109 110 124 106 124 SPILLED 121 125 124 131 135 146 107 110 111 119 SPILLED 118 130 107 112 113 SPILLED 97 120 112 121 116 128 107 121 107 129 SPILLED 120 125 SPILLED 139 143 113 111 117 113 133 123 135 107 120 108 SPILLED 94 118 132 135 120 129 111 Covance 6329-212 3M T-6316.1 PAGE: 44 WEEK 28 WEEK 32 131 111 133 SPILLED 123 139 123 142 153 108 111 118 113 119 120 152 103 119 105 SPILLED 90 131 114 133 109 134 110 133 115 143 SPILLED 129 139 125 150 157 111 120 118 119 112 134 151 139 124 110 187 95 137 131 133 118 130 119 1350 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 4 - 100 PPM C91346 127 134 130 137 136 146 143 138 C91347 106 112 106 110 112 119 109 120 C91348 97 98 96 89 102 105 103 104 C91349 110 108 111 118 119 117 122 128 C91351 129 122 121 130 122 134 125 131 C91352 118 108 115 117 128 128 138 130 C91353 108 112 108 117 111 112 118 127 C91354 95 96 92 97 93 102 SPILLED 100 C91355 153 137 116 NOT TAKEN C91356 131 119 113 112 123 119 143 146 C91357 94 84 85 84 87 88 96 86 C91358 130 114 120 118 126 131 133 133 C91359 SPILLED 142 176 135 136 111 121 127 C91360 103 101 102 103 106 104 112 118 C91361 87 102 106 103 105 101 110 114 C91362 141 150 116 SPILLED 112 109 135 135 C91363 120 126 124 131 126 117 136 130 C91364 99 99 95 102 94 98 104 102 C91365 232 SPILLED 228 228 SPILLED SPILLED SPILLED 215 C91366 116 103 106 122 110 113 121 122 C91367 125 114 113 125 118 115 130 141 C91368 103 107 102 96 102 101 107 106 C91369 118 109 124 119 120 126 SPILLED 130 C91370 110 110 110 NOT TAKEN C91371 SPILLED 155 145 120 126 111 134 SPILLED C91372 116 120 113 106 114 115 121 114 C91373 98 92 107 109 109 115 115 132 C91374 109 115 107 95 118 113 117 119 C91375 111 110 114 112 110 109 112 118 C91376 104 102 98 102 105 104 106 108 C91377 124 123 115 113 128 125 135 140 Covance 6329-212 3M T-6316.1 PAGE: 45 WEEK 28 WEEK 32 149 118 107 154 119 133 124 102 140 94 126 137 115 108 123 117 107 SPILLED 126 135 106 129 144 107 142 109 115 108 133 144 122 113 150 138 142 126 106 SPILLED 93 147 140 123 104 129 152 116 SPILLED 140 139 107 137 143 177 140 119 121 114 136 1351 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 4 - 100 PPM C91378 C91379 C91380 115 113 124 99 122 128 GROUP: FEMALE 6 - 100 PPM C91451 C91452 C91453 C91454 C91455 C91456 C91457 C91458 C91459 C91460 C91461 C91462 C91463 C91464 C91465 C91466 C91467 C91468 C91469 C91470 C91471 C91472 C91473 C91474 C91475 113 119 114 109 116 101 117 105 SPILLED 157 126 131 96 106 113 125 110 94 106 133 108 SPILLED 111 SPILLED 139 110 121 121 102 109 101 125 99 SPILLED 97 110 125 100 100 115 105 107 104 103 138 107 SPILLED 174 163 121 98 117 111 111 118 115 100 103 100 128 104 SPILLED 89 102 122 91 102 111 113 99 99 101 139 107 123 159 118 120 111 NOT TAKEN 126 111 126 108 115 131 115 102 103 121 98 SPILLED 96 115 133 100 99 111 112 110 98 109 134 112 112 SPILLED 124 120 108 117 126 112 105 104 124 112 SPILLED 95 118 128 108 104 112 112 107 92 102 142 116 136 107 131 129 105 119 114 122 124 104 112 102 128 106 SPILLED 90 133 127 107 98 118 114 103 SPILLED 100 134 114 123 94 127 127 112 132 119 123 121 111 115 108 135 117 SPILLED 108 145 133 108 124 106 123 115 SPILLED 108 137 119 122 104 146 138 114 128 122 129 125 109 115 114 128 115 SPILLED 98 154 136 109 107 134 121 115 SPILLED 111 144 127 119 119 132 139 Covance 6329-212 3M T-6316.1 PAGE: 46 WEEK 28 WEEK 32 112 108 124 128 125 129 134 118 111 111 125 118 SPILLED 101 155 131 115 115 139 116 122 109 109 135 128 117 102 153 133 126 146 130 120 118 116 133 125 SPILLED 104 153 143 117 115 148 125 130 153 122 151 130 121 SPILLED 127 142 1352 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 6 - 100 PPM C91476 111 111 112 115 108 114 128 130 C91477 123 115 112 124 121 122 122 135 C91478 134 138 121 125 136 146 137 144 C91479 133 133 126 132 130 138 144 138 C91480 111 105 103 109 105 104 109 111 C91481 92 91 93 95 102 96 100 102 C91482 116 112 110 122 118 111 117 120 C91483 107 107 98 108 109 120 133 115 C91484 110 106 107 111 113 106 95 110 C91485 105 103 96 83 98 96 109 111 C91486 107 109 104 114 112 112 118 122 C91487 111 105 96 99 100 98 115 125 C91488 124 121 129 121 128 116 125 122 C91489 136 117 120 118 119 129 141 127 C91490 117 113 114 111 116 112 121 118 Covance 6329-212 3M T-6316.1 PAGE: 47 WEEK 28 WEEK 32 137 131 139 143 146 SPILLED 144 SPILLED 120 130 101 110 118 138 122 133 124 124 116 116 134 130 124 132 123 126 141 130 119 123 1353 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 1 - 0 PPM C90711 192 199 182 188 176 174 179 183 C90712 184 200 160 186 185 188 191 178 C90713 228 228 225 229 210 191 203 208 C90714 187 205 190 189 186 C90715 189 204 192 198 187 189 193 190 C90716 170 182 168 163 170 170 169 178 C90720 198 210 191 186 186 165 187 183 C90721 244 69 NOT TAKEN C90722 176 187 176 173 175 171 172 170 C90723 214 233 212 218 207 196 201 C90724 180 196 183 180 188 191 198 193 C90726 183 189 179 179 176 182 181 177 C90727 201 205 192 194 189 187 190 204 C90728 190 207 187 201 197 191 199 192 C90729 189 188 185 170 175 175 180 180 C90730 208 214 207 211 207 208 181 63 C90733 204 215 202 200 203 215 SPILLED 224 C90734 231 SPILLED 242 235 226 218 228 201 C90735 186 200 179 181 179 176 177 159 C90736 203 221 199 197 200 195 202 172 C90738 185 196 193 191 185 187 186 182 C90739 184 192 186 173 187 185 186 169 C90740 207 211 207 204 209 181 155 67 C90741 189 192 187 187 184 186 178 180 C90742 183 192 173 172 165 172 180 170 C90743 209 225 208 200 205 204 210 210 C90744 211 226 217 210 218 203 216 213 C90745 219 233 213 211 217 202 202 195 C90747 202 206 183 191 187 182 187 179 C90749 175 198 181 173 170 176 168 163 C90750 165 179 174 176 174 177 173 183 C90751 216 221 210 199 198 Covance 6329-212 3M T-6316.1 PAGE: 48 WEEK 68 WEEK 72 177 103 176 203 212 221 198 217 181 190 199 203 180 192 190 197 185 183 204 206 189 194 187 187 45 211 207 232 218 183 190 117 NOT TAKEN 183 163 185 177 182 200 176 177 212 201 220 223 193 198 152 206 176 183 196 179 1354 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 1 - 0 PPM C90752 200 211 215 198 198 C90754 217 231 218 209 222 C90755 241 255 260 247 235 233 238 233 C90757 200 212 203 198 206 210 217 130 C90758 209 211 209 196 193 188 188 187 C90759 176 188 169 170 169 169 168 170 C90760 197 218 212 215 209 208 214 212 C90761 187 200 183 178 176 170 181 177 C90762 163 135 149 172 146 154 160 171 C90763 175 186 175 172 183 169 178 181 C90764 124 186 176 180 176 167 179 173 C90765 219 227 199 200 208 212 212 197 C90766 196 212 190 196 196 156 195 C90767 172 200 183 190 191 C90770 195 198 201 188 189 185 183 186 C90771 181 202 191 184 183 175 177 174 C90772 186 205 196 188 191 150 103 C90773 200 220 203 210 216 213 223 143 C90774 176 182 171 177 178 166 178 174 C90776 215 230 222 211 214 214 221 224 C90777 219 240 219 220 207 222 203 220 C90778 199 199 198 186 183 171 178 170 C90779 190 223 207 203 199 182 195 198 GROUP: MALE 2 - 3 PPM C90781 189 197 183 188 182 186 179 164 C90782 185 204 188 184 178 183 186 189 C90783 157 179 168 159 156 162 158 158 C90784 182 201 185 177 174 174 179 171 C90785 203 228 203 214 196 197 200 195 Covance 6329-212 3M T-6316.1 PAGE: 49 WEEK 68 WEEK 72 242 239 54 189 191 169 192 219 195 184 183 169 176 185 186 177 177 207 213 197 185 185 190 67 NOT TAKEN 231 237 227 243 179 183 189 184 191 187 180 203 154 169 181 188 198 209 1355 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 2 - 3 PPM C90786 178 191 179 175 160 160 172 187 C90787 202 212 193 200 196 191 205 198 C90788 190 207 188 188 186 179 195 187 C90790 217 232 223 215 203 218 207 222 C90791 186 206 184 184 182 184 190 174 C90792 201 209 195 193 180 10 C90793 177 188 187 186 181 175 185 180 C90794 SPILLED SPILLED 222 213 195 195 206 191 C90795 184 187 180 173 102 20 C90796 199 216 208 202 200 170 C90798 198 211 190 194 190 195 199 190 C90799 171 178 169 168 168 163 169 169 C90800 190 208 197 193 194 198 198 205 C90801 203 222 207 165 231 209 213 205 C90802 189 192 178 187 179 171 177 163 C90803 203 223 204 202 201 195 206 SPILLED C90804 208 214 204 200 194 194 198 204 C90805 208 207 195 192 218 50 18 C90808 210 230 215 198 202 201 219 34 C90809 187 205 178 187 192 187 187 183 C90810 201 226 212 210 200 190 216 210 C90811 194 205 182 227 210 185 154 179 C90812 191 215 200 193 189 178 188 193 C90815 181 205 189 180 191 168 183 174 C90816 209 218 193 203 222 211 227 204 C90819 193 220 211 201 203 193 201 210 C90820 183 196 186 183 174 178 184 195 C90821 222 232 221 228 206 205 215 219 C90822 191 201 181 175 177 170 173 170 C90823 180 198 184 188 171 179 171 174 C90824 189 214 193 190 184 180 192 187 Covance 6329-212 3M T-6316.1 PAGE: 50 WEEK 68 WEEK 72 170 178 206 206 193 195 198 226 189 193 183 177 194 212 217 217 171 178 210 215 201 209 182 191 222 243 199 197 32 186 189 217 222 189 195 206 199 182 182 211 221 211 228 76 222 219 171 150 167 196 188 196 1356 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 2 - 3 PPM C90825 192 210 197 192 210 194 193 189 C90826 267 SPILLED 234 239 238 230 230 135 C90827 208 220 211 221 223 230 214 228 C90828 205 216 210 230 251 191 204 208 C90829 234 239 228 219 219 209 216 211 C90830 183 205 185 181 180 174 178 186 C90832 195 219 187 203 202 182 191 190 C90835 175 181 184 189 197 174 189 192 C90837 169 174 159 160 187 238 275 229 C90838 233 198 232 224 226 197 221 226 C90839 224 249 222 222 217 209 209 221 C90840 200 227 207 191 205 199 208 217 GROUP: MALE 3 - 30 PPM C90841 201 195 191 184 198 179 189 193 C90842 150 153 148 144 148 139 149 135 C90844 204 234 208 203 214 181 218 207 C90845 179 197 174 181 186 181 185 192 C90847 189 197 190 182 181 171 186 183 C90848 194 223 220 204 214 197 209 186 C90849 195 198 199 199 200 195 186 164 C90850 159 179 159 155 166 164 169 160 C90851 168 183 169 172 174 168 182 169 C90854 208 247 204 197 193 189 204 194 C90855 197 202 179 186 182 171 179 166 C90856 183 202 186 184 174 165 193 184 C90857 182 201 181 200 185 181 177 184 C90858 179 188 185 181 178 175 172 168 C90859 212 228 213 214 215 178 156 79 C90860 179 175 161 166 162 164 166 152 Covance 6329-212 3M T-6316.1 PAGE: 51 WEEK 68 WEEK 72 206 221 NOT TAKEN 212 221 186 201 207 SPILLED 230 232 209 200 230 201 239 195 62 190 306 242 213 201 193 207 146 154 216 223 195 207 197 202 200 178 136 107 165 187 175 190 210 205 176 186 182 195 187 194 174 176 188 157 172 1357 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 3 - 30 PPM C90861 199 193 191 184 189 182 197 131 C90862 166 177 167 162 162 163 163 74 C90864 187 195 179 176 173 174 168 181 C90865 174 181 168 174 173 162 172 163 C90867 170 192 175 166 171 174 176 173 C90868 202 219 203 193 207 206 196 196 C90869 198 214 202 198 205 202 206 204 C90870 226 234 199 203 202 204 205 205 C90871 162 179 166 162 169 163 174 160 C90872 174 196 180 176 175 173 179 177 C90873 198 204 189 181 SPILLED 193 186 SPILLED C90874 200 214 202 195 200 31 231 225 C90875 182 197 189 190 186 177 196 188 C90876 SPILLED 235 218 210 216 NOT TAKEN C90878 170 183 164 165 172 C90879 207 205 199 207 185 187 198 201 C90881 197 216 205 200 197 188 202 198 C90882 193 203 188 193 196 187 194 192 C90883 207 239 226 225 152 223 217 177 C90884 198 205 195 171 185 190 200 SPILLED C90885 190 204 195 188 186 177 184 198 C90886 212 231 216 222 206 222 219 185 C90887 200 232 212 209 212 212 198 196 C90888 186 213 187 164 169 167 179 206 C90889 166 183 173 170 177 157 180 170 C90892 154 170 159 155 157 149 156 167 C90893 181 209 187 199 201 191 186 180 C90894 192 200 205 204 198 186 190 180 C90895 185 204 182 187 183 175 175 171 C90896 195 207 195 198 207 212 177 120 C90897 208 220 207 200 205 200 200 194 Covance 6329-212 3M T-6316.1 PAGE: 52 WEEK 68 WEEK 72 76 93 180 175 170 193 161 195 167 178 167 195 216 232 193 195 SPILLED 189 215 0 197 176 146 193 173 169 57 201 175 169 193 59 224 165 170 187 139 206 197 228 207 184 SPILLED 184 222 234 180 88 192 198 189 NOT TAKEN 207 1358 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 53 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 3 - 30 PPM C90898 178 195 166 176 174 191 30 C90899 172 187 170 171 178 179 177 168 168 170 C90900 189 204 189 185 187 184 178 169 170 177 GROUP: MALE 4 - 100 PPM C90901 172 186 176 175 181 185 178 190 196 118 C90902 171 181 169 173 173 C90903 157 170 163 162 151 153 158 155 168 166 C90904 170 184 170 162 163 158 160 162 161 161 C90906 207 222 206 197 194 193 186 175 195 208 C90908 173 193 167 173 183 190 179 192 194 197 C90909 207 212 215 204 199 C90910 172 184 171 163 163 157 158 167 168 173 C90911 177 188 182 176 181 175 171 167 176 177 C90912 192 205 188 190 188 187 185 102 127 116 C90913 165 181 164 170 159 C90914 190 196 188 183 194 193 186 197 212 210 C90915 208 211 208 206 211 206 202 204 215 218 C90916 174 186 174 175 176 170 161 166 173 178 C90918 203 202 196 191 180 C90919 189 190 193 188 192 175 173 174 188 192 C90920 172 190 176 182 188 192 195 184 200 218 C90922 189 193 182 176 176 155 100 NOT TAKEN C90923 202 189 197 188 191 196 191 189 196 212 C90924 168 180 188 184 187 C90925 186 197 195 179 183 186 184 179 194 198 C90926 211 219 216 215 220 191 189 180 190 205 C90927 183 194 162 167 170 164 167 158 172 182 C90928 166 179 157 156 155 169 163 159 157 172 C90929 177 178 147 150 144 182 149 174 NOT TAKEN 1359 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 54 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 4 - 100 PPM C90930 184 190 177 168 177 177 173 164 185 208 C90931 184 201 192 186 168 175 179 176 165 183 C90932 194 202 187 176 174 C90933 192 198 176 180 146 189 170 182 153 184 C90934 183 187 182 172 197 187 194 183 140 194 C90936 177 160 190 184 154 178 182 114 151 195 C90937 189 229 196 197 183 189 187 180 178 200 C90938 192 197 191 183 188 176 187 184 36 C90940 208 237 217 232 218 220 117 NOT TAKEN C90941 197 219 185 201 205 201 207 212 200 220 C90943 212 203 SPILLED 181 181 205 204 SPILLED 186 185 C90944 156 168 161 159 149 152 160 155 150 161 C90945 172 174 NOT TAKEN C90946 125 187 121 154 177 189 153 171 186 182 C90947 175 200 178 178 182 170 173 177 167 181 C90948 179 196 174 170 177 172 170 173 171 175 C90950 191 217 194 202 184 C90951 177 188 167 170 176 C90952 209 229 202 191 155 74 C90953 159 170 164 162 168 167 166 167 174 175 C90954 184 196 186 182 178 168 168 120 69 C90955 184 195 183 181 182 180 182 190 194 192 C90956 180 204 179 184 182 188 189 187 161 187 C90957 177 165 166 181 176 C90958 197 155 182 192 173 192 94 200 211 212 C90959 192 205 193 187 186 184 179 190 185 196 C90962 203 217 199 201 192 199 194 177 76 C90963 183 200 182 183 188 185 173 71 86 86 C90964 207 214 199 197 201 234 213 136 127 SPILLED C90965 209 207 171 188 171 179 181 129 SPILLED SPILLED C90967 183 196 183 175 171 1360 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 55 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 4 - 100 PPM C90968 181 199 173 172 172 159 170 177 2 224 C90969 213 230 231 225 216 211 216 202 221 207 C90970 163 173 172 157 168 154 170 157 181 173 GROUP: MALE 6 - 100 PPM C91041 197 211 210 204 201 134 2 C91042 188 190 180 191 175 183 200 194 204 205 C91043 194 205 189 178 170 186 190 186 185 198 C91044 194 213 193 200 196 147 85 NOT TAKEN C91045 200 214 196 157 C91047 162 189 150 170 169 169 183 173 179 186 C91048 193 208 199 204 204 201 200 200 196 198 C91049 212 45 C91050 189 213 192 190 195 206 205 200 199 203 C91051 213 218 199 212 207 207 210 202 202 225 C91052 229 237 226 229 240 248 175 45 NOT TAKEN C91053 172 182 172 182 179 194 187 179 188 195 C91054 192 203 194 191 198 193 217 213 223 226 C91055 225 211 218 206 198 216 224 202 233 247 C91056 193 SPILLED 189 187 183 184 176 192 186 195 C91057 183 197 189 177 170 185 199 203 175 127 C91058 169 183 175 166 168 155 182 175 167 191 C91059 167 186 168 187 196 188 191 201 206 202 C91060 169 181 167 172 165 172 188 185 196 206 C91061 174 187 169 174 172 171 187 189 184 196 C91062 215 199 184 193 183 185 199 190 194 190 C91063 155 151 143 144 144 140 158 154 153 150 C91064 188 202 199 188 187 171 185 198 194 193 C91065 169 178 158 152 161 126 148 138 135 158 C91066 182 188 182 184 180 189 206 190 180 170 1361 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 6 - 100 PPM C91067 199 202 185 186 186 201 202 196 C91069 213 226 201 208 201 219 228 216 C91070 172 188 184 172 163 171 183 182 C91071 188 198 186 192 184 184 207 208 C91072 154 172 166 158 157 160 140 158 C91073 194 208 206 209 214 222 194 185 C91074 165 177 180 171 163 166 180 180 C91075 194 206 200 202 191 193 194 200 C91076 178 193 175 187 185 173 194 198 C91077 196 207 197 182 180 185 188 151 C91078 165 150 141 167 168 141 170 174 C91079 172 193 177 188 182 188 196 204 C91080 186 185 174 178 183 185 182 173 GROUP: FEMALE 1 - 0 PPM C91121 151 162 151 134 158 C91122 139 135 131 150 152 C91123 132 148 138 136 128 140 118 144 C91124 142 148 160 142 144 C91125 129 145 135 135 135 116 128 137 C91127 118 155 132 137 147 138 159 151 C91128 161 196 193 170 180 167 144 179 C91130 127 150 160 130 138 151 130 148 C91131 111 118 107 107 103 111 114 116 C91132 201 174 154 147 167 158 146 141 C91133 163 144 134 139 142 C91134 170 175 182 165 171 184 170 169 C91136 170 173 168 175 168 159 168 158 C91137 110 113 104 85 100 91 116 115 C91138 168 182 124 129 119 116 117 108 Covance 6329-212 3M T-6316.1 PAGE: 56 WEEK 68 WEEK 72 199 198 217 254 185 179 213 220 167 160 236 228 182 184 199 206 199 197 188 204 176 174 189 158 196 198 141 144 142 151 150 171 217 196 154 143 118 123 141 122 29 59 153 183 125 134 123 113 1362 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 1 - 0 PPM C91139 C91141 C91142 C91144 C91146 C91147 C91148 C91149 C91150 C91151 C91152 C91153 C91154 C91156 C91157 C91158 C91159 C91161 C91164 C91166 C91167 C91168 C91170 C91172 C91173 C91175 C91176 C91177 C91178 C91179 C91180 140 143 166 142 148 149 36 144 149 162 149 138 134 124 126 137 155 155 143 141 134 119 138 109 114 114 SPILLED 108 112 112 111 148 168 154 143 156 135 99 149 205 SPILLED 188 167 163 160 160 173 134 129 145 143 134 135 143 136 140 156 164 142 156 138 155 148 158 170 160 162 150 164 141 114 187 222 208 204 207 188 187 184 151 153 149 148 145 174 205 178 196 193 207 198 198 138 151 150 140 134 146 130 136 153 171 200 179 181 194 191 176 147 143 124 123 127 121 123 124 163 164 163 134 132 131 149 147 126 136 126 114 123 137 121 128 148 162 134 142 136 62 7 171 190 188 185 151 192 184 181 128 140 142 136 138 131 144 142 156 185 168 156 144 164 156 141 125 141 141 136 146 140 134 142 159 169 163 143 179 159 130 133 200 SPILLED 202 171 171 159 170 145 110 169 159 153 117 64 156 SPILLED 188 146 153 166 146 171 150 155 155 139 148 160 145 160 103 147 114 115 120 119 120 150 153 160 154 141 141 134 137 137 153 168 138 145 154 162 178 181 SPILLED SPILLED NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 57 WEEK 68 WEEK 72 154 127 134 0 109 111 116 72 152 105 155 159 139 137 122 122 116 15 200 185 134 129 170 148 128 117 162 168 125 130 188 190 142 139 184 172 160 163 140 142 176 167 156 191 171 185 167 170 140 150 1363 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 1 - 0 PPM C91182 164 175 166 157 160 163 157 173 C91183 SPILLED SPILLED SPILLED 199 231 196 SPILLED 178 C91184 145 180 162 149 163 171 165 177 C91185 124 171 164 165 160 149 53 8 C91186 126 152 162 158 144 143 156 130 C91187 148 169 155 147 156 155 138 108 C91188 138 141 138 132 123 141 139 137 C91189 115 122 118 114 118 114 117 111 C91190 143 151 137 145 137 146 138 128 GROUP: FEMALE 2 - 3 PPM C91191 137 166 186 113 SPILLED 175 149 182 C91193 165 146 152 141 114 130 141 118 C91194 148 164 148 154 139 95 133 126 C91195 170 177 150 172 179 151 153 172 C91196 116 154 157 148 140 149 160 150 C91197 185 208 200 170 154 133 153 144 C91199 140 153 141 143 145 141 149 146 C91200 132 176 126 130 127 117 124 137 C91201 150 159 136 138 140 144 120 163 C91202 164 179 150 160 174 159 170 180 C91203 145 160 153 149 148 131 165 169 C91204 156 166 159 190 160 161 85 C91206 169 170 155 168 167 157 175 116 C91207 143 146 141 139 143 100 151 125 C91208 155 157 138 138 152 137 134 127 C91209 172 184 143 136 151 134 129 140 C91210 152 154 148 135 135 131 156 142 C91211 131 154 147 127 133 147 131 150 C91212 144 148 142 130 137 148 150 2 Covance 6329-212 3M T-6316.1 PAGE: 58 WEEK 68 WEEK 72 189 164 190 184 178 173 146 165 88 76 143 137 122 120 159 144 189 179 131 140 158 166 174 151 172 164 145 148 149 159 148 120 152 158 158 168 156 167 118 162 117 131 98 84 153 191 140 144 165 171 1364 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91224 C91225 C91226 C91227 C91228 C91229 C91231 C91232 C91234 C91235 C91236 C91238 C91239 C91240 C91242 C91243 C91244 C91245 C91246 C91247 C91249 C91250 FEMALE 2 - 3 PPM 125 162 146 143 121 166 SPILLED 132 159 143 141 147 128 144 121 134 124 140 SPILLED 162 149 144 143 192 261 99 146 SPILLED 179 163 123 160 172 158 143 143 188 227 153 169 169 142 167 136 154 135 154 136 171 181 168 147 132 135 SPILLED 208 142 149 SPILLED 203 173 128 155 148 120 137 145 192 168 137 174 143 126 150 126 154 125 174 120 186 152 155 141 146 110 SPILLED 153 131 158 SPILLED 194 165 114 154 147 115 125 121 171 161 129 160 112 133 148 128 146 126 165 127 186 144 147 145 135 139 147 176 130 158 SPILLED 180 162 117 161 148 164 119 139 139 144 128 142 132 131 151 130 149 127 147 111 170 123 148 138 141 SPILLED SPILLED 140 140 141 SPILLED 180 160 133 131 137 124 125 179 142 129 137 135 127 148 127 SPILLED 117 161 123 170 115 144 143 128 104 144 140 141 138 SPILLED 150 164 143 147 149 135 138 SPILLED 144 136 151 128 136 158 117 170 114 149 127 180 132 145 140 129 152 146 147 153 SPILLED 167 171 152 138 157 145 131 187 137 126 161 120 125 159 130 159 122 149 137 100 127 142 17 124 SPILLED 167 105 148 161 91 167 140 Covance 6329-212 3M T-6316.1 PAGE: 59 WEEK 68 WEEK 72 134 173 149 NOT TAKEN SPILLED 162 141 155 133 121 159 128 163 125 156 139 142 145 NOT TAKEN 141 SPILLED 166 147 175 117 184 155 76 185 149 SPILLED 147 156 159 141 136 151 144 182 150 158 143 151 156 144 146 160 171 SPILLED 70 170 154 1365 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 3 - 30 PPM C91252 126 145 151 146 144 136 120 137 C91253 142 143 130 138 130 108 125 139 C91254 117 124 121 115 117 107 112 125 C91255 128 139 136 131 128 135 130 118 C91256 142 152 142 130 128 139 136 132 C91257 131 133 124 128 126 128 125 127 C91258 140 155 151 136 125 131 141 134 C91259 147 164 161 134 127 68 71 C91260 152 166 164 158 154 156 161 136 C91261 136 149 153 150 144 148 144 140 C91262 118 132 114 124 125 123 125 123 C91263 160 167 167 161 157 155 153 87 C91264 123 142 132 134 127 126 123 122 C91265 136 129 119 143 138 119 145 136 C91266 177 181 164 162 151 154 161 156 C91267 126 144 133 139 138 145 170 163 C91268 129 135 131 131 137 119 132 125 C91269 143 186 165 167 165 178 158 161 C91270 147 167 156 90 140 99 C91271 149 191 173 158 160 164 183 168 C91273 144 151 159 152 141 145 136 146 C91274 127 142 138 132 139 150 141 143 C91275 150 166 178 153 145 130 104 SPILLED C91276 141 149 134 136 128 127 131 122 C91277 134 136 129 123 SPILLED 126 133 132 C91278 141 145 134 125 125 123 123 132 C91279 209 213 201 192 190 197 SPILLED 195 C91280 145 156 141 135 145 148 149 144 C91282 161 201 173 90 10 C91283 147 150 155 138 144 148 147 129 C91284 143 139 150 108 123 129 118 125 Covance 6329-212 3M T-6316.1 PAGE: 60 WEEK 68 WEEK 72 148 147 141 138 129 121 123 128 143 157 109 110 145 142 58 85 132 141 142 144 159 NOT TAKEN 125 125 127 139 173 178 139 170 180 178 198 165 159 155 132 145 131 130 141 NOT TAKEN 131 131 250 212 153 152 142 156 155 142 1366 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C91286 C91289 C91290 C91292 C91294 C91295 C91296 C91297 C91298 C91300 C91301 C91302 C91303 C91305 C91306 C91307 C91308 C91309 C91310 GROUP: C91311 C91312 C91313 C91314 C91315 C91319 C91320 C91321 C91323 FEMALE 3 - 30 PPM 159 131 187 126 123 115 118 180 142 148 110 149 135 177 146 138 SPILLED 158 129 164 164 195 153 141 132 141 SPILLED 213 170 148 158 141 185 158 146 208 171 153 FEMALE 4 - 100 PPM 139 154 114 120 153 156 200 226 131 145 148 155 141 146 144 170 167 183 139 131 184 142 139 140 132 151 175 152 141 155 152 187 156 130 191 163 151 125 108 148 165 138 143 137 158 173 129 138 SPILLED 132 128 123 106 80 87 128 169 161 67 142 143 158 161 145 136 103 128 123 121 145 137 128 137 140 123 121 116 113 118 151 142 153 162 136 164 SPILLED SPILLED SPILLED 118 146 135 140 106 89 141 148 145 139 146 159 140 68 157 143 144 145 147 134 162 173 184 168 162 174 143 134 150 151 136 143 131 141 145 137 180 167 151 132 125 161 156 154 154 131 154 144 141 142 136 129 124 122 124 NOT TAKEN 113 107 105 108 106 147 141 144 143 133 226 164 190 SPILLED SPILLED 142 141 137 136 138 137 134 136 133 123 144 145 147 142 143 155 159 154 158 145 166 150 Covance 6329-212 3M T-6316.1 PAGE: 61 WEEK 68 WEEK 72 150 131 84 111 163 165 140 162 135 155 122 134 139 143 118 140 94 98 167 163 90 NOT TAKEN 163 147 178 174 143 142 151 145 113 146 149 112 113 139 139 204 114 135 134 117 150 140 169 157 1367 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C91324 C91325 C91326 C91327 C91328 C91330 C91331 C91332 C91334 C91335 C91336 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91346 C91347 C91348 C91349 C91351 C91352 C91353 C91354 C91356 C91357 C91358 C91359 C91360 FEMALE 4 - 100 PPM 112 124 126 122 109 142 153 119 142 115 271 90 145 125 149 130 128 116 142 SPILLED 109 SPILLED 127 148 143 106 SPILLED 97 140 128 131 121 136 136 135 144 162 172 134 152 137 243 109 153 149 164 138 132 124 161 137 133 SPILLED 142 160 150 119 176 102 179 166 146 111 118 122 114 143 151 157 123 138 119 SPILLED 81 138 124 132 120 118 125 150 127 137 SPILLED 150 141 120 111 167 91 163 134 130 114 111 126 123 140 147 151 112 139 117 SPILLED 103 142 143 147 120 124 79 144 136 142 SPILLED 130 155 144 105 153 91 170 134 123 112 94 123 122 130 138 155 117 134 110 SPILLED 97 139 150 149 130 121 98 140 131 136 SPILLED 146 156 135 106 SPILLED 95 167 134 123 105 111 124 125 105 155 159 118 128 101 SPILLED 86 134 137 148 103 103 147 181 140 106 191 91 154 131 120 109 121 118 125 137 152 159 SPILLED 123 116 SPILLED 93 135 142 155 119 116 136 155 100 194 95 161 126 126 118 121 112 121 140 142 117 128 115 SPILLED 95 149 126 145 134 104 127 151 83 160 105 157 122 119 Covance 6329-212 3M T-6316.1 PAGE: 62 WEEK 68 WEEK 72 119 121 140 130 128 113 128 144 150 150 139 127 128 124 132 140 116 129 205 SPILLED 113 101 146 148 141 127 152 155 135 112 114 113 104 NOT TAKEN 153 167 88 94 179 161 93 108 146 155 143 154 120 120 1368 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 4 - 100 PPM C91361 112 119 119 122 117 125 127 124 C91362 135 156 146 135 149 128 138 147 C91363 151 138 132 132 130 127 136 135 C91364 113 129 111 116 113 110 115 103 C91365 SPILLED SPILLED 188 211 197 C91366 140 155 138 144 153 147 155 140 C91367 154 163 139 127 140 C91368 121 131 110 126 133 C91369 138 164 138 149 141 142 158 148 C91371 154 170 156 221 160 C91372 118 131 115 122 112 111 123 112 C91373 167 183 166 165 178 149 151 133 C91374 122 136 126 125 138 117 119 125 C91375 117 134 126 132 127 117 127 117 C91376 120 129 119 119 118 115 113 114 C91377 143 134 118 137 137 141 134 129 C91378 113 129 105 115 112 134 131 161 C91380 124 143 134 137 127 131 127 119 GROUP: FEMALE 6 - 100 PPM C91451 152 140 126 121 126 145 129 120 C91452 146 160 153 143 142 154 172 155 C91453 143 163 145 135 133 148 145 125 C91454 121 127 116 112 108 114 137 120 C91455 125 134 121 124 121 128 126 117 C91456 117 138 122 119 117 140 141 139 C91457 134 149 136 130 127 144 145 138 C91458 127 142 126 137 125 142 128 C91459 SPILLED SPILLED 224 SPILLED SPILLED SPILLED SPILLED 184 C91460 106 120 100 102 90 90 69 Covance 6329-212 3M T-6316.1 PAGE: 63 WEEK 68 WEEK 72 116 114 158 164 134 140 112 117 151 165 173 174 120 119 2 133 135 140 140 72 120 145 161 SPILLED 121 129 143 152 166 138 164 169 113 121 129 132 147 143 154 156 171 174 1369 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 6 - 100 PPM C91461 142 169 157 161 156 179 188 183 C91462 140 162 151 140 139 149 143 138 C91463 129 136 118 122 126 134 149 137 C91464 121 136 119 126 131 137 140 126 C91465 145 169 154 146 142 176 166 152 C91466 128 155 129 137 143 136 148 159 C91467 131 150 140 142 139 145 144 140 C91468 SPILLED SPILLED 154 123 139 150 SPILLED C91469 123 134 119 119 123 131 135 125 C91470 156 180 150 165 151 150 161 128 C91471 136 157 138 143 138 146 152 133 C91472 122 139 126 135 123 135 143 145 C91473 116 147 108 107 118 121 135 120 C91474 147 172 128 139 136 136 SPILLED 146 C91475 161 171 162 159 147 159 194 167 C91476 144 158 140 144 136 142 162 157 C91477 145 155 141 147 135 143 149 156 C91478 149 166 159 167 156 170 SPILLED 155 C91479 147 177 154 140 147 174 167 153 C91480 134 146 139 139 127 129 134 126 C91481 110 128 124 130 127 136 157 131 C91482 138 152 130 140 132 141 155 148 C91483 136 147 129 131 92 132 145 138 C91484 127 127 133 123 123 108 120 74 C91485 121 134 122 113 92 134 133 122 C91486 136 141 133 138 137 152 177 SPILLED C91487 125 144 123 136 130 159 161 163 C91488 133 139 132 135 121 132 149 142 C91489 141 163 142 149 143 154 177 165 C91490 SPILLED 152 137 141 138 144 160 136 Covance 6329-212 3M T-6316.1 PAGE: 64 WEEK 68 WEEK 72 188 184 161 158 145 155 128 84 161 182 178 163 155 168 93 NOT TAKEN 0 147 153 129 157 136 143 166 161 176 170 152 189 162 182 173 180 157 171 139 141 172 113 162 162 141 144 43 137 155 176 157 180 189 158 178 170 192 133 201 1370 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 1 - 0 PPM C90711 38 C90712 184 183 182 167 162 158 116 SPILLED C90713 211 211 199 154 129 107 C90715 212 196 181 126 109 C90716 195 193 203 193 164 162 171 166 C90720 200 196 179 152 C90722 189 188 195 183 204 192 208 184 C90724 200 209 216 199 189 208 193 191 C90726 134 180 168 158 CNTMD SPILLED SPILLED SPILLED C90727 204 211 200 188 130 38 C90728 198 185 186 195 189 176 160 155 C90729 184 191 187 179 NOT TAKEN C90733 65 216 SPILLED 191 SPILLED C90734 170 NOT TAKEN C90735 190 198 199 178 60 191 204 SPILLED C90738 159 183 172 181 182 151 C90739 190 175 199 171 199 186 50 C90741 194 186 177 192 125 37 NOT TAKEN C90742 192 179 175 168 171 162 150 155 C90743 222 234 209 201 198 189 182 186 C90744 223 198 163 C90745 25 C90747 167 171 SPILLED 194 181 155 170 175 C90749 180 179 170 181 24 C90750 191 190 183 170 176 156 171 160 C90755 241 241 229 218 194 181 NOT TAKEN C90758 203 SPILLED 235 199 170 157 163 SPILLED C90759 163 161 71 C90760 154 118 SPILLED C90761 185 194 190 176 169 C90762 167 C90763 184 202 136 1371 Covance 6329-212 3M T-6316.1 PAGE: 65 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 1 - 0 PPM C90764 C90765 C90770 C90771 C90776 C90777 C90778 C90779 177 228 197 190 234 205 182 177 181 226 195 205 226 210 175 190 GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90790 C90791 C90793 C90794 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90809 195 192 195 171 172 176 164 167 190 189 171 188 213 217 199 196 140 61 190 199 131 138 SPILLED 233 240 183 177 201 198 179 SPILLED 139 3 116 191 194 206 157 SPILLED NOT TAKEN 227 122 169 230 173 190 173 186 51 68 230 NOT TAKEN 174 121 224 NOT TAKEN 103 239 189 3 190 200 180 193 SPILLED 85 161 222 188 207 221 197 SPILLED 231 167 236 287 187 SPILLED 187 164 162 131 206 165 199 190 186 SPILLED 182 150 218 280 172 SPILLED 183 174 160 87 219 160 181 197 193 224 183 161 228 SPILLED 162 SPILLED 180 132 145 193 200 185 190 SPILLED 193 155 217 137 227 204 NOT TAKEN 183 203 193 188 154 188 183 145 138 136 32 131 145 189 186 176 172 186 SPILLED 38 158 148 96 66 195 SPILLED 1372 Covance 6329-212 3M T-6316.1 PAGE: 66 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 2 - 3 PPM C90810 C90811 C90812 C90815 C90816 C90819 C90821 C90822 C90823 C90824 C90825 C90826 C90828 C90829 C90830 C90832 C90835 C90838 C90839 C90840 SPILLED 201 195 188 179 211 222 0 175 196 209 239 183 249 192 206 208 237 203 197 225 203 193 181 189 239 226 179 24 206 233 NOT TAKEN 232 189 167 214 235 166 SPILLED GROUP: MALE 3 - 3 0 PPM C90841 C90842 C90844 C90845 C90847 C90848 C90849 C90850 201 147 235 119 199 NOT TAKEN 81 183 209 155 226 201 194 222 196 198 173 43 239 229 194 217 253 245 211 221 182 240 229 SPILLED 189 157 228 149 184 204 178 185 156 208 219 164 201 211 223 175 187 197 223 SPILLED SPILLED 149 125 210 137 183 199 190 173 164 NOT TAKEN 225 137 135 NOT TAKEN 216 186 160 196 202 138 SPILLED 205 175 139 138 216 157 192 216 162 191 176 213 159 SPILLED 116 41 128 122 208 197 112 31 143 98 191 182 112 106 NOT TAKEN 163 175 184 97 120 178 248 176 199 159 207 SPILLED 167 150 190 160 188 SPILLED 129 137 161 120 142 116 1373 Covance 6329-212 3M T-6316.1 PAGE: 67 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 3 - 30 PPM C90851 190 185 184 163 173 184 179 170 C90854 208 204 199 177 169 162 163 112 C90855 177 193 182 160 168 157 160 165 C90856 189 198 209 187 193 180 162 180 C90857 179 184 203 180 176 137 87 C90858 160 186 162 148 127 SPILLED 168 117 C90860 176 169 177 154 150 152 151 159 C90864 164 178 179 160 160 150 165 172 C90865 173 170 173 166 165 150 153 144 C90867 183 196 181 175 169 159 168 172 C90869 217 217 224 211 196 177 164 191 C90870 117 NOT TAKEN C90871 149 181 170 160 163 144 156 156 C90872 200 201 214 136 C90875 196 196 202 191 C90879 123 37 C90881 225 225 217 214 218 201 212 199 C90882 208 226 226 208 220 204 194 196 C90883 185 179 225 175 165 189 144 161 C90884 210 SPILLED 193 176 202 177 168 84 C90885 192 176 184 151 194 SPILLED 200 148 C90886 231 172 98 92 C90888 159 1 C90889 190 171 197 178 192 176 165 168 C90892 1 C90893 203 204 197 189 200 199 196 195 C90894 191 180 194 162 159 147 C90895 182 190 190 174 165 C90897 215 208 218 203 211 C90899 186 179 180 189 177 160 97 NOT TAKEN C90900 179 183 173 162 170 161 148 156 Covance 6329-212 3M T-6316.1 PAGE: 68 1374 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 4 - 100 PPM C90903 C90904 C90906 C90908 C90910 C90911 C90912 C90914 C90915 C90916 C90919 C90920 C90923 C90925 C90926 C90927 C90928 C90930 C90931 C90933 C90934 C90936 C90937 C90941 C90943 C90944 C90946 C90947 C90948 C90953 C90955 166 168 166 168 176 196 191 187 169 180 173 190 121 90 213 222 221 220 191 188 166 214 217 215 162 207 202 208 207 187 191 168 180 169 184 193 192 184 165 192 SPILLED 205 SPILLED 196 190 180 109 146 SPILLED 157 165 78 190 169 183 195 181 177 182 207 179 164 191 205 186 185 212 212 181 209 56 188 210 186 35 171 157 167 192 206 198 81 177 165 181 174 177 204 156 156 152 115 151 136 136 142 123 198 205 197 108 177 173 SPILLED 180 167 160 178 126 183 180 168 159 155 169 168 165 138 91 NOT TAKEN 207 195 158 168 156 156 158 175 214 218 178 NOT TAKEN 164 26 162 197 103 SPILLED SPILLED 178 148 160 176 174 64 164 123 126 173 165 173 174 129 199 171 199 183 163 186 171 167 123 188 208 185 126 170 151 140 120 85 NOT TAKEN 159 146 147 161 148 186 177 182 181 176 151 158 SPILLED 154 SPILLED 162 171 154 161 162 183 187 160 164 165 Covance 6329-212 3M T-6316.1 PAGE: 69 1375 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 4 - 100 PPM C90956 204 218 205 193 181 166 160 SPILLED C90958 55 163 SPILLED 173 139 C90959 187 207 136 C90965 SPILLED 190 SPILLED 181 176 158 175 176 C90968 193 SPILLED 83 161 107 111 SPILLED 151 C90969 218 210 150 C90970 180 185 193 159 177 187 NOT TAKEN GROUP: MALE 6 - 100 PPM C91042 201 205 218 196 99 C91043 184 193 200 C91047 176 165 185 148 116 173 158 164 C91048 116 NOT TAKEN C91050 225 217 219 199 143 164 160 C91051 217 212 113 2 C91053 186 192 C91054 210 226 230 228 214 186 220 176 C91055 239 222 C91056 196 201 184 184 171 163 142 149 C91058 172 187 C91059 173 31 C91060 202 204 212 190 169 159 167 173 C91061 188 193 192 129 C91062 177 52 102 42 155 C91063 150 152 159 148 80 45 NOT TAKEN C91064 190 193 205 181 162 192 C91065 168 157 143 169 159 173 197 161 C91066 127 173 154 C91067 189 209 209 206 193 163 NOT TAKEN C91069 239 252 NOT TAKEN 1376 Covance 6329-212 3M T-6316.1 PAGE: 70 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 6 - 100 PPM C91070 C91071 C91072 C91073 C91074 C91075 C91076 C91077 C91078 C91079 C91080 164 221 164 228 195 112 213 194 184 186 185 176 225 159 216 173 224 168 197 141 192 GROUP: FEMALE 1 - 0 PPM C91123 C91125 C91127 C91130 C91131 C91132 C91136 C91137 C91138 C91141 C91144 C91147 C91148 C91149 C91150 C91153 C91154 170 154 157 56 165 136 175 150 149 177 108 61 155 168 NOT TAKEN 206 131 146 160 159 157 142 168 151 164 NOT TAKEN 126 171 SPILLED 189 172 223 140 177 206 173 NOT TAKEN 190 75 201 171 167 158 156 162 SPILLED 147 165 131 195 144 163 221 119 213 196 222 205 197 159 162 175 143 121 63 117 138 114 157 NOT TAKEN 148 214 113 SPILLED SPILLED 218 188 171 174 104 153 127 142 136 110 172 141 214 129 185 SPILLED SPILLED 146 199 123 SPILLED SPILLED SPILLED 105 SPILLED NOT TAKEN 160 130 103 139 108 171 113 115 68 152 107 149 134 193 SPILLED SPILLED 171 7 125 144 102 144 1377 Covance 6329-212 3M T-6316.1 PAGE: 71 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 1 - 0 PPM C91157 C91158 C91159 C91164 C91166 C91167 C91168 C91170 C91172 C91175 C91176 C91177 C91178 C91182 C91183 C91184 C91186 C91188 C91189 C91190 134 130 135 130 119 112 112 126 193 193 181 163 161 146 169 151 153 152 138 134 152 196 196 154 150 87 143 174 149 152 145 146 144 142 208 153 SPILLED 149 SPILLED 152 147 182 185 NOT TAKEN 118 150 78 SPILLED 246 SPILLED 213 176 SPILLED 225 153 194 186 192 121 169 196 199 192 176 94 SPILLED 72 180 179 179 168 141 172 164 158 95 185 77 5 NOT TAKEN 197 205 207 200 195 185 176 193 206 198 166 46 163 177 166 147 89 145 156 145 165 158 128 133 139 143 124 129 144 126 65 153 174 157 166 164 169 151 120 GROUP: FEMALE 2 - 3 PPM C91191 176 177 173 170 205 99 C91193 119 SPILLED 138 62 C91194 178 SPILLED 163 135 135 115 53 NOT TAKEN C91195 166 169 180 156 104 55 NOT TAKEN C91196 150 22 C91197 144 164 164 162 166 153 143 154 C91199 147 177 155 163 88 106 C91200 148 155 149 Covance 6329-212 3M T-6316.1 PAGE: 72 1378 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 2 - 3 PPM C91201 C91202 C91203 C91207 C91211 C91213 C91214 C91216 C91218 C91219 C91220 C91221 C91224 C91225 C91226 C91227 C91228 C91229 C91231 C91232 C91235 C91236 C91240 C91242 C91243 C91245 C91246 C91249 164 185 NOT TAKEN 141 152 117 181 159 173 179 174 163 131 140 112 157 194 80 166 140 SPILLED 152 148 SPILLED 178 175 133 195 166 189 158 168 180 145 144 146 178 166 137 55 85 145 SPILLED 163 179 153 SPILLED 169 SPILLED 181 169 161 191 151 121 146 160 140 SPILLED 63 63 164 164 149 NOT TAKEN 176 137 194 101 184 148 SPILLED 169 SPILLED 193 174 117 181 161 135 162 165 SPILLED 96 NOT TAKEN 134 167 138 117 107 101 134 153 130 131 141 165 164 155 152 47 177 188 130 129 177 169 97 126 78 26 NOT TAKEN 151 136 145 134 157 101 21 103 149 160 135 142 SPILLED 170 176 99 132 133 138 141 137 158 NOT TAKEN 22 65 136 151 147 149 153 132 143 126 139 129 129 126 147 140 150 129 134 145 121 132 123 137 128 Covance 6329-212 3M T-6316.1 PAGE: 73 1379 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C91252 C91253 C91254 C91256 C91257 C91258 C91261 C91262 C91264 C91265 C91266 C91268 C91269 C91271 C91273 C91276 C91278 C91279 C91280 C91283 C91284 C91286 C91292 C91294 C91295 C91296 C91297 C91300 C91301 C91303 C91305 FEMALE 3 - 30 PPM 149 144 122 SPILLED 102 123 135 133 127 163 175 143 63 192 160 124 140 200 162 151 143 136 171 175 151 125 136 119 151 164 175 164 157 118 170 89 168 146 137 159 148 159 168 48 143 104 143 135 SPILLED 162 183 155 138 172 171 177 76 150 SPILLED 162 165 159 157 162 130 178 11 130 122 149 152 169 143 98 136 131 SPILLED 157 148 150 142 186 169 157 63 140 182 153 155 152 148 124 158 113 1 138 119 155 165 121 130 120 205 155 2 135 116 165 SPILLED 145 163 173 129 164 126 107 150 114 136 42 144 160 137 131 132 SPILLED 158 SPILLED 143 127 176 167 171 153 158 143 132 134 110 86 129 129 140 153 131 157 72 130 124 174 166 151 148 135 137 142 129 136 121 139 109 125 119 82 55 NOT TAKEN 107 115 162 155 124 120 166 176 156 135 136 80 134 151 121 130 Covance 6329-212 3M T-6316.1 PAGE: 74 1380 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 3 - 30 PPM C91306 147 141 141 150 129 120 137 C91307 164 162 147 154 139 125 101 67 C91310 160 170 178 167 142 153 154 157 GROUP: FEMALE 4 - 100 PPM C91312 115 116 109 100 87 19 C91313 128 133 82 C91315 149 163 SPILLED SPILLED 150 148 149 145 C91320 106 C91321 162 168 154 111 103 C91324 132 134 129 98 120 109 116 108 C91325 130 152 135 136 128 105 54 C91326 105 84 86 C91327 140 144 157 135 173 161 150 141 C91330 171 130 61 C91331 38 C91332 197 SPILLED 150 114 118 136 94 106 C91334 143 147 132 130 107 101 152 C91335 125 136 134 126 127 114 101 113 C91336 SPILLED SPILLED SPILLED SPILLED 225 SPILLED 207 SPILLED C91338 110 107 122 116 104 96 107 88 C91339 160 176 160 141 84 102 C91340 140 155 139 133 135 124 83 150 C91341 160 148 167 166 155 170 148 174 C91342 136 133 145 114 131 133 125 125 C91344 123 140 118 116 104 69 91 C91351 157 161 127 124 55 C91354 99 95 97 NOT TAKEN C91356 205 228 201 172 158 147 157 79 C91357 105 102 101 96 91 101 95 92 1381 Covance 6329-212 3M T-6316.1 PAGE: 75 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 4 - 100 PPM C91359 C91360 C91361 C91362 C91363 C91364 C91366 C91372 C91374 C91375 C91376 C91378 C91380 150 129 141 128 125 163 164 140 123 108 118 147 147 123 134 150 152 153 81 113 126 158 SPILLED 125 122 GROUP: FEMALE 6 - 100 PPM C91451 C91453 C91454 C91455 C91456 C91457 C91459 C91461 C91462 C91463 C91465 C91466 C91467 C91471 C91472 148 148 149 132 2 161 186 185 164 146 153 168 166 156 141 165 168 99 132 167 159 219 154 153 99 197 139 157 170 133 118 185 154 97 128 137 150 125 SPILLED 150 157 154 143 188 188 98 151 138 69 164 162 152 164 112 105 176 138 85 112 122 111 168 121 158 193 132 173 176 142 SPILLED 156 179 147 167 123 121 116 115 106 109 161 162 153 132 SPILLED 102 95 140 116 103 110 75 31 114 112 119 189 SPILLED 181 139 182 178 146 161 142 140 218 241 108 120 113 167 NOT TAKEN 163 168 117 134 135 132 2 135 165 153 139 61 125 SPILLED 116 98 144 103 110 128 205 161 204 126 144 124 129 1382 Covance 6329-212 3M T-6316.1 PAGE: 76 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C91473 C91474 C91475 C91476 C91477 C91478 C91479 C91480 C91482 C91483 C91485 C91486 C91487 C91488 C91489 C91490 FEMALE 6 - 100 PPM 139 169 175 170 195 186 99 NOT TAKEN 198 109 174 161 174 171 149 155 131 91 151 141 151 154 129 75 176 197 151 159 174 183 160 153 168 114 156 93 151 170 163 93 132 160 175 173 148 140 149 154 79 98 177 178 146 181 142 77 148 142 SPILLED 195 150 162 155 79 119 149 NOT TAKEN 162 140 177 142 151 174 147 141 NOT TAKEN 144 155 173 110 146 117 120 166 174 143 178 Covance 6329-212 3M T-6316.1 PAGE: 77 1383 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 0.231 SPILLED 0.256 0.250 0.251 0.263 0.253 0.326 0.246 0.263 0.257 0.260 0.255 0.274 0.244 0.249 0.246 0.273 0.272 0.241 0.248 0.253 0.274 0.247 0.276 0.241 0.239 0.248 0.276 0.253 0.260 0.213 0.224 0.225 0.224 0.221 0.225 0.224 0.231 0.222 0.232 0.239 0.237 0.229 0.258 0.223 0.227 0.217 0.242 0.235 0.224 0.225 0.241 0.247 0.229 0.245 0.216 0.217 0.226 0.242 0.238 0.223 0.192 0.218 0.212 0.208 0.204 0.194 0.207 0.214 0.205 0.215 0.211 0.216 0.219 0.228 0.200 0.231 0.200 0.221 0.212 SPILLED 0.211 0.210 0.213 0.207 0.215 0.193 0.199 0.210 0.220 0.213 0.201 0.180 0.207 0.202 0.194 0.194 0.192 0.197 0.197 NOT TAKEN 0.197 0.207 0.214 0.201 0.211 0.193 0.209 0.185 0.145 0.195 0.186 0.203 0.203 0.199 0.192 0.209 0.197 0.188 0.196 0.208 0.197 0.196 0.171 SPILLED 0.190 0.181 0.181 0.181 0.188 0.182 NOT TAKEN 0.191 0.203 0.200 0.186 0.210 0.191 0.199 0.176 0.187 0.178 0.174 0.198 0.184 0.188 0.179 0.198 0.189 0.174 0.190 SPILLED 0.186 0.203 0.167 0.193 0.177 0.175 0.178 0.169 0.184 0.168 NOT TAKEN 0.179 0.186 0.183 0.173 0.187 0.176 0.187 0.167 0.169 0.170 0.169 0.186 0.176 0.174 0.174 0.188 0.177 0.159 0.175 0.180 0.174 0.185 0.159 0.221 0.163 0.162 0.173 0.164 0.179 0.162 NOT TAKEN 0.177 0.180 0.170 0.171 0.178 0.179 0.194 0.167 0.180 0.172 0.160 0.183 0.168 0.159 0.170 0.174 0.170 0.157 0.169 0.182 0.169 0.183 SPILLED 0.171 0.164 0.159 0.170 0.158 0.170 0.155 NOT TAKEN 0.170 0.172 0.165 0.163 0.172 0.176 0.189 0.157 0.155 0.164 0.157 0.179 0.163 0.155 0.156 0.167 0.165 0.152 0.164 0.164 0.156 0.170 0.153 0.181 0.153 0.151 0.158 0.140 0.161 0.147 NOT TAKEN 0.157 0.167 0.159 0.155 0.164 0.165 0.188 0.145 0.167 0.152 0.143 0.170 0.148 0.145 0.150 0.157 0.158 0.142 0.156 0.158 0.149 0.160 0.150 0.163 0.148 0.149 0.156 0.140 0.156 0.142 NOT TAKEN 0.154 0.159 0.147 0.146 0.156 0.160 0.182 0.143 0.146 0.146 0.139 0.167 0.146 0.139 0.144 0.155 0.155 0.144 0.150 0.164 0.144 0.160 1384 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 2 - 3 PPM C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 0.249 0.249 0.242 0.260 0.239 0.247 0.262 0.256 0.244 0.259 0.246 0.255 0.296 0.255 0.306 0.257 0.275 0.274 0.261 0.250 0.268 0.272 0.257 0.251 0.266 0.274 0.251 0.249 0.266 0.221 0.220 0.221 0.237 0.208 0.215 0.251 0.231 0.211 0.224 0.227 0.225 0.248 0.236 SPILLED 0.236 0.255 0.248 0.240 0.232 0.244 0.257 0.234 0.228 0.223 0.240 0.223 0.223 0.239 0.199 0.203 0.205 0.215 0.186 0.196 0.216 0.211 0.201 0.201 0.210 0.203 0.230 0.225 SPILLED 0.215 0.231 0.216 0.221 0.207 0.222 0.247 0.208 0.202 0.199 0.221 0.198 0.203 0.216 0.185 0.189 NOT TAKEN 0.197 0.188 NOT TAKEN 0.207 0.188 0.185 0.191 0.195 0.198 0.199 0.204 SPILLED 0.198 0.221 0.200 0.203 0.196 0.210 NOT TAKEN NOT TAKEN 0.196 0.202 0.210 0.189 0.194 0.201 0.169 0.184 NOT TAKEN 0.186 0.174 NOT TAKEN 0.200 0.177 0.178 0.175 0.181 0.184 0.181 0.190 SPILLED 0.187 0.199 0.185 0.196 0.185 0.190 NOT TAKEN NOT TAKEN 0.190 0.193 0.194 0.181 0.184 0.191 0.163 0.175 NOT TAKEN 0.177 0.167 NOT TAKEN 0.194 0.175 0.164 0.171 0.173 0.174 0.182 0.179 SPILLED 0.175 0.195 0.172 0.176 0.175 0.177 NOT TAKEN NOT TAKEN 0.177 0.182 0.190 0.170 0.175 0.178 0.160 0.169 NOT TAKEN 0.174 0.166 NOT TAKEN SPILLED 0.164 0.162 0.162 0.174 0.177 0.173 0.170 SPILLED 0.170 0.181 0.171 0.166 0.175 0.177 NOT TAKEN NOT TAKEN 0.195 0.177 0.178 0.166 0.166 0.174 0.154 NOT TAKEN NOT TAKEN 0.165 0.156 NOT TAKEN 0.178 0.157 0.151 0.154 0.163 0.171 0.166 0.159 SPILLED 0.158 0.182 0.161 0.164 0.173 0.162 NOT TAKEN NOT TAKEN 0.171 0.168 0.169 0.155 0.163 0.166 0.149 NOT TAKEN NOT TAKEN 0.157 0.156 NOT TAKEN SPILLED 0.146 0.146 0.144 0.156 0.165 0.151 0.155 SPILLED 0.152 0.175 0.153 0.155 0.163 0.151 NOT TAKEN NOT TAKEN 0.171 0.158 0.158 0.153 0.153 0.157 0.137 NOT TAKEN NOT TAKEN 0.154 0.152 NOT TAKEN 0.181 0.140 0.143 0.140 0.151 0.165 0.144 0.150 0.191 0.144 0.171 0.142 0.148 0.151 0.146 NOT TAKEN NOT TAKEN 0.155 0.155 0.150 0.147 0.144 0.148 1385 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 3 - 3 0 PPM C90841 C90842 C90843 C90844 C90845 C90846 C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 2.705 2.495 2.656 2.623 SPILLED 2.343 2.519 2.552 2.530 2.431 2.546 2.582 SPILLED 2.733 3.093 2.579 2.470 2.636 2.635 2.745 2.372 2.883 2.911 2.656 2.543 2.420 2.489 2.523 2.877 2.669 2.423 2.367 2.481 2.401 2.401 2.168 2.088 2.270 2.334 2.235 2.171 2.418 2.582 2.213 2.525 2.502 2.328 2.203 2.383 2.405 2.439 2.113 2.720 2.552 2.351 2.188 1.983 2.201 2.101 2.480 SPILLED 2.143 2.123 1.941 2.077 2.157 2.007 1.855 2.045 2.149 2.015 2.052 2.050 2.108 2.193 2.259 2.188 1.994 1.910 2.095 2.236 2.182 2.016 2.396 2.246 2.110 2.059 1.819 2.026 1.962 2.253 SPILLED 1.923 2.032 1.963 2.064 SPILLED 1.887 NOT TAKEN 1.908 2.057 1.833 1.909 1.993 1.929 NOT TAKEN 2.089 1.950 1.929 1.914 2.022 2.002 2.018 1.912 2.418 SPILLED 1.917 1.912 NOT TAKEN 1.849 1.873 2.042 SPILLED 1.841 2.032 1.851 1.813 1.967 1.822 NOT TAKEN 1.744 1.912 1.712 1.827 1.992 1.824 NOT TAKEN SPILLED 1.884 1.746 1.787 1.831 1.837 1.964 1.782 2.076 1.868 1.791 1.716 NOT TAKEN 1.779 1.776 1.885 1.889 1.755 1.952 1.709 1.793 SPILLED 1.741 NOT TAKEN 1.698 1.796 1.665 1.729 1.871 1.766 NOT TAKEN SPILLED 1.667 1.664 1.718 1.793 1.734 SPILLED 1.724 2.037 1.685 1.769 1.666 NOT TAKEN 1.690 1.704 1.700 1.781 1.644 1.852 1.705 SPILLED 1.819 1.670 NOT TAKEN 1.594 1.755 1.531 1.710 1.794 1.677 NOT TAKEN SPILLED 1.590 1.696 1.687 1.781 1.725 1.801 1.660 1.850 1.611 1.769 1.581 NOT TAKEN 1.685 1.613 1.655 1.680 1.551 1.733 1.559 1.670 SPILLED 1.610 NOT TAKEN 1.527 1.675 1.485 1.580 1.644 1.721 NOT TAKEN SPILLED 1.550 1.543 1.554 1.685 1.653 1.788 1.643 1.824 1.543 1.722 1.547 NOT TAKEN 1.568 1.568 1.582 1.603 1.424 1.655 1.590 1.631 1.805 1.515 NOT TAKEN 1.538 1.657 1.459 1.531 SPILLED 1.547 NOT TAKEN SPILLED 1.461 1.506 1.534 1.485 1.606 1.583 1.519 1.726 1.453 1.607 1.462 NOT TAKEN 1.573 1.507 1.510 1.531 1.384 1.579 1.477 1.520 1.690 1.451 NOT TAKEN 1.424 1.515 1.500 1.442 1.532 1.532 NOT TAKEN 1.606 1.427 1.511 1.490 1.492 1.572 1.593 1.493 1.749 1.369 1.524 1.467 NOT TAKEN 1.483 1.446 1.395 1.520 1.371 1386 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 3 - 3 0 PPM C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 2.263 2.380 2.447 2.639 2.715 2.451 2.609 2.731 2.672 2.637 2.551 2.699 2.777 2.743 2.751 2.522 2.378 2.628 2.654 2.646 2.487 2.933 2.704 3.101 2.818 2.531 2.540 2.615 2.606 SPILLED 2.087 2.143 2.307 2.287 2.160 2.361 2.234 2.336 2.332 2.223 2.577 2.399 2.327 2.522 2.219 2.102 2.243 2.335 2.410 2.169 2.543 2.327 2.843 SPILLED 2.280 2.267 2.358 2.253 1.905 1.909 1.949 2.137 2.106 2.018 2.153 2.152 2.161 2.126 2.042 2.171 2.244 2.101 2.115 2.026 1.944 2.033 2.109 2.129 2.058 2.310 2.055 2.453 SPILLED 2.062 2.025 2.113 2.070 1.836 1.881 1.837 1.934 1.826 1.942 2.165 2.000 1.903 1.984 1.924 SPILLED 1.926 1.851 2.060 1.865 1.858 1.761 NOT TAKEN NOT TAKEN 1.964 2.114 1.981 2.094 SPILLED 1.917 1.876 1.949 1.919 1.742 1.817 1.694 1.860 1.680 1.846 2.033 1.799 1.817 1.833 1.855 2.138 1.812 1.731 2.028 1.794 1.730 1.697 NOT TAKEN NOT TAKEN 1.943 2.012 1.918 2.233 1.854 1.854 1.820 1.849 1.778 1.710 1.735 1.604 1.724 1.525 1.692 1.916 1.674 1.714 1.661 1.788 1.999 1.742 1.589 1.835 1.653 1.585 1.610 NOT TAKEN NOT TAKEN 1.789 1.888 1.793 1.878 1.777 1.694 1.673 1.725 1.656 1.622 1.653 1.520 1.634 1.574 1.658 1.852 1.701 1.679 1.635 1.709 SPILLED 1.714 1.616 1.769 1.646 1.598 1.591 NOT TAKEN NOT TAKEN 1.783 1.850 1.796 1.872 SPILLED 1.686 1.726 1.816 1.661 1.547 1.526 1.489 1.594 1.517 1.608 1.833 1.648 1.626 1.535 1.649 1.863 1.686 1.542 1.731 1.634 1.534 1.560 NOT TAKEN NOT TAKEN 1.747 1.785 1.647 1.828 1.581 1.585 1.639 1.731 1.690 1.497 1.576 1.438 1.606 1.542 1.380 1.710 1.613 1.584 1.535 1.589 1.798 1.584 1.433 1.637 1.544 1.470 1.408 NOT TAKEN NOT TAKEN 1.676 1.652 1.577 1.897 SPILLED 1.500 1.507 1.640 1.570 1.406 1.506 1.396 1.447 1.452 1.560 1.634 1.548 1.478 1.456 1.532 1.810 1.641 1.412 1.525 1.517 1.431 1.358 NOT TAKEN NOT TAKEN 1.612 1.573 1.490 1.709 1.864 1.398 1.482 1.509 1.492 1387 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 8.445 8.946 8.627 8.516 8.621 8.318 7.838 8.440 8.524 8.988 8.993 7.651 9.250 8.937 8.666 8.131 8.815 7.768 9.171 8.431 8.561 8.280 8.098 8.845 8.640 8.212 9.193 7.975 8.733 9.311 7.917 7.312 7.534 7.402 7.154 SPILLED 7.085 6.744 7.250 7.684 7.583 7.562 6.683 8.170 7.597 7.578 7.343 7.625 7.025 7.776 7.441 7.568 6.961 7.027 7.758 SPILLED 7.664 8.157 7.073 8.026 7.726 6.756 6.442 6.531 6.673 6.671 7.098 6.568 6.345 6.851 6.843 7.088 6.865 6.303 7.030 6.865 7.220 6.749 7.153 6.377 7.407 6.842 6.665 6.672 6.593 7.353 6.936 7.048 7.213 6.597 6.868 6.946 6.385 6.282 6.429 6.409 6.362 6.877 6.434 6.076 6.445 6.375 6.618 6.345 5.991 6.524 6.425 6.712 6.197 6.373 5.982 6.704 6.552 5.970 5.965 6.045 6.623 6.245 6.412 6.740 6.125 6.577 6.452 6.424 5.987 5.974 6.078 5.778 6.495 6.186 5.844 6.010 5.727 6.362 6.133 5.727 6.030 6.040 6.458 5.949 6.062 5.727 6.379 6.021 5.974 5.729 5.680 6.345 5.848 6.083 6.308 5.685 6.274 5.474 6.108 5.815 5.601 5.847 5.495 6.003 5.701 5.434 5.644 5.404 6.087 5.694 5.330 5.216 5.533 5.917 5.505 5.577 5.380 5.796 5.619 5.542 5.419 5.445 5.842 5.630 5.634 5.857 5.370 5.896 5.932 5.863 5.427 5.226 5.417 5.442 5.757 5.491 5.032 5.273 5.503 5.821 5.323 5.211 3.090 5.413 5.363 5.116 5.383 5.278 5.541 5.550 5.330 5.113 5.232 5.880 5.316 5.422 5.601 5.216 5.575 5.766 5.377 5.120 5.174 5.089 5.104 5.714 5.183 4.837 5.068 5.378 5.663 5.088 4.800 4.920 5.073 5.396 5.189 5.261 5.200 5.413 5.292 5.145 5.133 5.276 5.623 5.384 5.273 5.306 4.966 5.679 5.602 5.142 Covance 6329-212 3M T-6316.1 PAGE: 5 WEEK 9 WEEK 10 4.818 4.713 4.924 4.909 5.465 5.170 4.704 4.834 4.872 5.229 5.146 4.881 6.099 4.934 4.946 4.896 4.900 4.803 5.259 5.181 4.825 4.590 4.962 5.106 4.976 4.867 4.963 4.673 5.480 5.458 4.928 4.959 4.783 4.607 4.839 5.374 4.879 4.721 4.876 4.857 5.181 4.970 4.591 5.690 4.833 4.951 4.801 4.498 4.550 4.899 4.890 4.790 4.620 5.056 5.161 4.989 4.641 4.666 4.587 5.338 5.396 4.629 1388 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 4 - 100 PPM C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 8.973 9.104 8.719 8.553 9.524 9.069 9.305 8.858 9.250 9.975 8.775 SPILLED 8.291 8.292 8.392 8.220 10.399 10.628 8.780 8.129 9.089 8.148 9.563 9.058 8.418 8.783 8.625 8.603 9.006 8.911 9.060 7.469 7.807 7.526 7.328 7.897 9.570 8.163 7.501 7.391 7.613 7.681 8.011 7.314 7.342 6.955 7.199 7.687 8.039 7.559 7.143 7.828 7.131 8.106 7.777 7.099 7.635 7.100 7.499 8.770 7.719 7.807 7.097 7.171 6.774 6.585 7.399 6.939 7.477 6.947 6.945 7.055 6.849 SPILLED 6.812 6.633 SPILLED 6.672 6.901 6.997 6.883 6.379 7.204 6.653 7.556 7.152 6.545 6.959 6.534 6.918 7.112 SPILLED 6.937 6.497 6.806 6.192 NOT TAKEN 6.137 6.634 7.015 NOT TAKEN 6.510 SPILLED NOT TAKEN SPILLED 6.213 6.208 6.122 6.231 6.530 6.288 6.094 5.403 6.427 6.378 6.565 6.410 6.091 6.649 6.313 6.198 6.669 NOT TAKEN 6.620 5.838 6.281 6.131 NOT TAKEN 6.408 5.570 6.443 NOT TAKEN 6.046 6.312 NOT TAKEN 7.161 5.690 5.812 5.907 5.858 6.286 6.622 5.708 5.742 5.762 5.756 6.379 5.961 5.809 6.299 6.135 5.978 6.369 NOT TAKEN 6.204 5.934 5.935 5.743 NOT TAKEN 6.143 6.353 6.128 NOT TAKEN 5.691 5.770 NOT TAKEN SPILLED 5.487 5.427 5.488 5.653 6.008 5.571 5.605 5.635 5.341 5.495 5.934 5.322 5.531 5.919 5.676 5.701 5.907 NOT TAKEN 5.825 5.760 5.702 5.353 NOT TAKEN 5.905 5.628 6.028 NOT TAKEN 5.515 5.672 NOT TAKEN 6.195 5.369 5.333 5.138 5.351 5.814 5.155 5.517 5.510 5.220 5.337 5.919 5.252 5.425 4.571 5.155 5.548 6.218 NOT TAKEN 5.460 5.612 5.503 5.201 NOT TAKEN 5.760 5.177 5.874 NOT TAKEN 5.478 5.348 NOT TAKEN 5.740 5.406 5.177 5.008 5.071 5.708 5.874 5.389 5.438 5.128 5.228 5.809 5.127 5.308 5.523 5.198 5.420 5.480 NOT TAKEN 5.418 5.233 5.019 5.642 NOT TAKEN 5.369 SPILLED 5.514 NOT TAKEN 5.147 4.901 NOT TAKEN 5.290 5.145 4.875 4.492 5.044 5.328 5.273 4.786 4.887 4.637 4.887 5.180 5.005 4.800 5.423 4.613 4.885 4.991 NOT TAKEN 4.981 5.050 4.969 5.329 NOT TAKEN 5.234 5.260 5.207 NOT TAKEN 4.766 4.950 NOT TAKEN 5.862 4.823 4.543 4.674 4.970 4.997 4.351 4.743 4.870 4.467 4.620 4.848 4.934 3.184 5.014 4.513 4.878 4.898 NOT TAKEN 4.876 1389 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 4 - 100 PPM C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 8.353 9.111 9.543 8.157 8.070 8.350 8.542 8.484 7.164 7.924 8.321 7.359 7.226 7.389 7.477 7.215 GROUP: MALE 5 - 300 PPM C90971 C90972 C90973 C90974 C90975 C90976 C90977 C90978 C90979 C90980 C90981 C90982 C90983 C90984 C90985 C90986 C90987 C90988 C90989 C90990 24.897 20.706 24.808 25.267 26.653 27.721 25.432 24.512 23.634 27.166 26.854 24.967 25.557 24.262 25.895 24.342 24.055 33.527 25.567 24.296 19.850 18.227 21.264 20.896 23.673 21.962 20.537 21.359 20.679 23.140 22.885 22.093 26.346 20.370 22.768 21.695 20.428 24.531 21.526 21.635 6.681 7.117 7.675 6.637 6.505 6.604 6.611 6.753 18.417 17.651 18.980 18.554 21.984 19.378 19.657 SPILLED 18.023 21.085 20.190 19.887 21.556 19.102 19.017 19.075 17.787 21.156 19.114 20.566 6.244 6.401 7.035 NOT TAKEN 6.479 6.637 6.276 6.791 18.157 16.301 18.088 17.883 19.486 18.348 17.348 17.554 16.619 19.545 18.255 17.681 19.286 NOT TAKEN 17.683 19.035 16.680 17.620 17.543 17.918 5.955 6.118 6.354 NOT TAKEN 6.285 6.211 5.943 6.564 17.300 14.907 18.068 16.338 18.949 16.706 SPILLED 16.515 16.667 18.886 18.027 16.183 17.431 NOT TAKEN 17.376 SPILLED 16.122 SPILLED 16.327 17.722 5.485 5.653 5.989 NOT TAKEN 5.924 5.948 5.727 5.685 18.141 14.198 17.016 15.729 18.666 15.731 SPILLED 15.233 15.332 17.486 16.853 16.610 17.512 NOT TAKEN 16.158 16.024 15.636 SPILLED 15.126 17.032 5.406 5.402 5.016 NOT TAKEN 5.390 5.633 5.353 SPILLED 17.113 14.701 15.594 15.184 18.575 15.783 SPILLED 14.844 16.064 17.354 16.187 15.229 17.113 NOT TAKEN 16.493 15.672 16.051 SPILLED 15.236 16.271 5.164 5.117 5.504 NOT TAKEN 5.232 5.383 5.578 5.488 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.826 4.867 5.172 NOT TAKEN 4.894 4.935 5.182 5.234 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.691 4.717 4.934 NOT TAKEN 4.732 4.716 5.319 5.044 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1390 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 8 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 5 - 3 0 0 PPM C90991 C90992 C90993 C90994 C90995 C90996 C90997 C90998 C90999 C91000 C91001 C91002 C91003 C91004 C91005 C91006 C91007 C91008 C91009 C91010 C91011 C91012 C91013 C91014 C91015 C91016 C91017 C91018 C91019 C91020 C91021 25.091 24.000 24.047 24.103 24.685 24.470 24.576 23.800 23.363 26.094 24.759 23.361 24.675 29.210 31.440 24.370 24.854 SPILLED 25.700 25.794 26.590 24.272 25.304 26.774 24.832 23.935 23.826 25.210 27.995 27.327 24.057 21.903 20.421 20.759 20.297 EXCLUDE 20.414 21.152 21.504 20.104 21.653 21.075 20.249 20.092 SPILLED 23.498 21.390 22.391 27.505 23.245 22.533 22.681 20.430 22.262 25.779 21.395 20.695 19.674 21.181 23.505 23.083 22.342 19.325 18.058 19.402 18.769 19.839 17.940 18.367 20.152 18.340 19.205 19.572 18.609 18.059 19.770 21.838 19.112 20.145 24.595 18.742 20.023 20.694 18.953 20.540 21.651 20.042 19.002 17.819 18.997 20.433 SPILLED 19.996 19.007 15.966 17.694 17.664 17.982 16.719 17.421 18.990 16.586 17.603 19.242 17.813 17.632 18.690 NOT TAKEN 17.590 19.171 SPILLED 16.437 NOT TAKEN 18.955 17.517 17.955 SPILLED 17.842 17.666 17.847 16.951 18.872 19.242 19.008 16.586 15.355 17.316 16.475 17.395 15.702 16.924 18.815 15.847 18.031 17.692 16.649 15.663 SPILLED NOT TAKEN 17.470 18.418 SPILLED 16.332 EXCLUDE 17.545 16.784 17.453 18.142 17.514 16.841 17.046 16.511 18.275 18.860 17.500 15.869 15.674 15.831 16.136 15.504 15.527 16.860 18.649 15.106 16.474 17.741 16.709 15.736 17.679 NOT TAKEN 16.873 18.237 SPILLED 16.841 16.792 16.210 15.119 16.583 17.421 16.741 15.584 16.079 15.120 17.691 18.331 16.625 16.146 15.276 15.120 15.566 16.065 15.453 16.404 18.098 15.275 15.983 16.246 16.064 NOT TAKEN 16.974 NOT TAKEN 15.150 17.187 SPILLED 16.185 16.722 15.982 15.652 17.065 17.167 16.906 16.146 16.152 14.570 16.162 17.366 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1391 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 5 - 3 0 0 PPM C91022 C91023 C91024 C91025 C91026 C91027 C91028 C91029 C91030 C91031 C91032 C91033 C91034 C91035 C91036 C91037 C91038 C91039 C91040 24.975 25.810 25.887 NOT TAKEN 24.031 24.490 24.905 24.653 26.674 22.800 27.968 24.734 27.086 26.294 23.688 24.695 29.116 26.418 24.899 21.691 22.175 22.316 21.486 20.201 21.664 20.620 21.502 23.060 20.615 23.377 21.289 22.295 21.801 18.583 21.202 20.652 22.697 21.705 GROUP: MALE 6 - 100 PPM C91041 C91042 C91043 C91044 C91045 C91046 C91047 C91048 C91049 8.997 9.153 8.759 8.618 9.329 9.105 8.949 8.178 9.269 7.508 7.721 7.504 7.177 7.889 7.625 7.980 7.333 7.972 19.021 20.039 19.305 19.177 SPILLED 19.143 18.429 18.699 20.294 18.678 20.546 19.163 20.647 19.245 19.123 18.727 18.852 19.923 20.530 6.757 7.090 7.036 6.884 7.192 7.003 7.188 6.497 7.114 18.782 19.355 NOT TAKEN 17.103 17.513 18.750 18.401 18.423 19.867 18.001 18.918 NOT TAKEN 19.790 18.559 17.298 NOT TAKEN 18.283 19.505 19.304 6.271 6.529 6.843 6.716 6.868 6.337 6.701 6.077 6.639 17.488 17.664 NOT TAKEN 16.336 17.120 17.040 17.448 17.017 17.989 16.105 18.890 NOT TAKEN 18.639 18.801 16.421 NOT TAKEN 16.827 18.763 18.739 5.864 6.117 6.341 6.286 6.200 6.147 6.068 5.843 6.166 16.811 17.618 NOT TAKEN 15.949 16.289 16.546 16.000 17.192 18.104 15.990 17.558 NOT TAKEN 17.691 17.650 15.617 NOT TAKEN 16.374 16.571 17.522 5.603 5.887 6.223 5.958 5.968 4.511 5.920 5.999 5.941 16.115 16.307 NOT TAKEN 14.998 16.092 15.112 15.548 NOT TAKEN 17.638 13.825 15.668 NOT TAKEN 16.364 16.504 15.105 NOT TAKEN 15.214 14.954 17.406 5.259 5.490 5.870 5.636 5.374 NOT TAKEN 5.573 5.570 5.685 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 5.144 5.315 5.883 5.519 5.007 NOT TAKEN 3.410 5.569 5.614 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 5.004 5.300 5.426 5.086 4.858 NOT TAKEN 4.936 5.493 5.411 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.775 5.393 5.401 5.329 4.690 NOT TAKEN 5.353 5.368 5.166 1392 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 6 - 100 PPM C91050 C91051 C91052 C91053 C91054 C91055 C91056 C91057 C91058 C91059 C91060 C91061 C91062 C91063 C91064 C91065 C91066 C91067 C91068 C91069 C91070 C91071 C91072 C91073 C91074 C91075 C91076 C91077 C91078 C91079 C91080 9.707 8.865 9.218 SPILLED 9.401 9.159 8.530 7.952 8.715 8.498 11.011 8.030 9.093 9.421 8.432 9.376 8.317 8.673 8.925 9.597 8.287 8.553 9.016 8.163 9.058 9.149 8.212 8.939 8.432 8.316 8.626 8.232 7.563 9.125 8.245 8.106 7.777 7.589 7.101 7.369 7.392 SPILLED 7.208 9.200 8.501 7.262 8.025 7.369 7.521 7.453 8.123 7.098 7.603 7.838 7.253 7.713 8.357 7.197 7.717 7.225 7.350 7.342 7.845 7.183 7.354 6.799 7.202 7.095 6.730 6.332 6.596 6.839 7.166 6.621 7.369 7.361 6.894 7.328 6.764 6.860 6.474 7.346 6.708 6.923 7.244 6.649 7.099 7.880 6.669 7.132 6.646 6.828 6.828 7.446 6.703 7.093 6.210 6.682 6.826 6.511 6.120 6.398 6.593 6.994 6.164 6.783 6.580 6.648 6.812 6.115 6.341 5.808 6.599 6.366 6.424 6.552 5.921 6.687 7.065 6.088 6.566 6.206 6.547 6.497 6.624 6.244 SPILLED 5.808 6.211 6.197 5.900 5.721 5.850 6.295 6.765 5.789 6.397 6.613 6.429 6.482 5.895 5.945 5.408 6.494 5.952 6.038 6.081 5.721 6.112 7.152 5.679 6.263 5.685 6.197 6.289 6.441 5.843 6.100 5.596 5.702 5.831 5.550 5.471 5.675 5.819 6.391 5.430 6.031 6.036 5.850 6.203 5.407 5.801 4.693 6.326 5.631 5.726 5.811 5.445 5.781 6.314 4.542 5.961 5.355 5.912 5.867 5.862 5.665 5.781 6.011 5.487 5.621 5.231 5.172 5.100 5.519 6.178 5.305 5.766 5.520 5.564 5.803 5.400 5.536 4.892 5.976 5.418 5.354 5.578 5.153 5.587 6.091 4.713 5.769 4.988 5.677 5.734 5.867 5.556 5.645 SPILLED 5.324 5.490 3.941 5.128 4.982 5.429 5.996 5.200 5.489 5.602 5.328 5.517 5.448 5.399 4.607 5.943 5.242 5.283 5.398 5.139 5.260 5.817 5.660 5.410 4.827 5.365 5.592 Covance 6329-212 3M T-6316.1 PAGE: 10 WEEK 9 WEEK 10 4.909 5.590 5.854 4.961 4.856 5.023 5.666 4.786 4.651 5.075 5.529 5.005 5.152 4.971 4.913 5.183 5.040 5.085 1.897 5.465 5.007 5.054 5.054 4.699 5.026 5.351 5.317 5.252 4.511 4.941 5.353 5.064 5.489 5.190 4.808 4.510 4.937 5.510 4.613 4.308 5.020 5.507 4.873 4.820 4.786 4.841 4.977 4.936 4.872 2.659 5.216 4.868 4.877 4.900 4.819 4.832 5.340 5.135 5.031 4.478 4.833 5.140 1393 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 11 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 7 - 3 0 0 PPM C91081 C91082 C91083 C91084 C91085 C91086 C91087 C91088 C91089 C91090 C91091 C91092 C91093 C91094 C91095 C91096 C91097 C91098 C91099 C91100 C91101 C91102 C91103 C91104 C91105 C91106 C91107 C91108 C91109 C91110 C91111 SPILLED 24.176 25.378 23.153 SPILLED 23.981 25.314 24.697 27.211 22.989 24.880 25.369 23.870 25.200 24.320 22.812 25.730 23.754 25.866 25.525 23.983 25.040 26.267 26.748 24.207 23.629 25.787 27.042 26.087 26.204 24.901 22.053 20.510 21.467 20.045 23.239 20.583 21.680 21.978 22.983 21.601 21.429 20.893 20.168 21.129 20.041 20.658 20.816 20.739 20.438 22.153 20.530 27.843 21.974 24.620 22.452 20.515 21.995 23.579 23.252 22.254 21.466 19.728 18.418 19.613 17.937 20.160 19.991 18.358 20.311 20.408 19.452 19.226 18.719 18.803 18.597 SPILLED 18.459 18.694 19.323 19.495 19.643 18.850 20.849 20.401 21.304 18.158 18.386 19.521 21.258 20.085 21.131 19.391 23.859 17.062 18.407 16.706 19.770 18.553 16.866 18.595 19.188 17.710 19.103 17.900 14.074 16.770 18.618 17.170 17.191 18.057 21.504 18.656 18.527 18.617 19.226 19.048 16.912 15.451 18.455 19.585 18.616 18.650 17.996 17.552 16.544 17.775 16.698 16.881 17.498 16.117 16.848 18.398 17.481 17.808 16.358 18.185 16.598 18.175 17.168 16.568 17.188 16.789 17.267 16.930 19.103 18.423 18.833 16.650 17.689 17.506 17.912 17.831 18.175 17.695 16.568 15.365 18.139 16.349 15.467 16.278 14.870 15.882 18.034 16.446 16.426 16.552 17.824 16.889 17.734 16.173 15.625 16.140 NOT TAKEN 16.186 17.040 18.780 17.799 17.863 16.707 16.229 16.967 17.535 18.279 17.574 17.812 15.746 15.449 17.093 16.188 13.984 15.424 14.625 15.563 16.327 16.786 15.667 14.934 16.685 15.841 15.807 15.407 15.158 15.964 NOT TAKEN 16.298 17.523 17.143 16.432 16.892 16.479 16.725 16.309 16.765 16.184 15.988 17.304 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1394 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 12 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 7 - 3 0 0 PPM C91112 C91113 C91114 C91115 C91116 C91117 C91118 C91119 C91120 26.190 26.503 25.605 25.227 23.390 27.090 26.607 25.788 26.823 22.487 21.467 21.087 21.971 19.972 SPILLED 21.872 22.338 22.313 GROUP: FEMALE 2 - 3 PPM C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 0.264 0.319 SPILLED 0.277 0.306 0.280 0.312 0.276 0.381 0.283 0.269 0.275 0.308 0.304 0.325 SPILLED 0.306 0.294 0.267 0.249 0.285 SPILLED 0.257 0.291 0.279 0.297 0.243 0.353 0.269 SPILLED 0.265 0.297 0.281 0.307 0.262 0.274 0.268 0.244 20.140 19.847 19.237 20.285 18.201 SPILLED 20.018 20.106 20.741 0.227 0.254 0.565 0.250 0.263 0.265 0.270 0.247 0.298 0.291 0.246 0.238 0.281 0.266 0.298 0.244 0.238 0.241 0.239 18.599 17.586 16.631 18.399 16.450 21.853 18.269 18.297 18.855 0.223 0.284 SPILLED 0.235 0.248 0.228 0.291 NOT TAKEN SPILLED 0.337 0.271 0.240 0.270 0.258 NOT TAKEN 0.229 0.263 SPILLED 0.216 17.955 16.603 16.633 16.637 16.881 SPILLED 17.640 17.921 18.274 0.232 0.232 SPILLED 0.237 0.227 0.214 0.293 NOT TAKEN 0.310 0.256 0.227 0.208 0.246 0.252 NOT TAKEN 0.226 0.245 0.232 0.206 17.081 15.572 16.034 16.775 15.899 18.449 18.319 17.182 14.926 0.205 0.255 SPILLED 0.245 0.234 0.204 SPILLED NOT TAKEN SPILLED 0.233 0.279 0.193 0.219 0.261 NOT TAKEN 0.216 0.235 0.233 0.208 16.464 17.062 15.029 15.873 18.320 SPILLED 15.177 16.327 3.588 0.232 0.225 SPILLED 0.231 0.235 0.209 SPILLED NOT TAKEN SPILLED 0.194 0.215 0.185 0.202 0.235 NOT TAKEN 0.213 0.233 0.213 0.196 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.212 0.237 SPILLED 0.213 0.208 0.185 0.221 NOT TAKEN SPILLED 0.225 SPILLED 0.175 0.197 0.282 NOT TAKEN 0.204 0.225 SPILLED 0.198 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.204 0.213 SPILLED 0.214 0.188 0.169 0.229 NOT TAKEN SPILLED 0.228 0.180 0.168 0.174 0.210 NOT TAKEN 0.188 0.213 0.194 0.183 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.190 0.202 0.258 0.210 0.192 0.167 0.271 NOT TAKEN SPILLED 0.241 0.199 0.167 0.168 0.234 NOT TAKEN 0.194 0.198 0.214 0.188 1395 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 13 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 2 - 3 PPM C91210 C91211 C91212 C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 0.453 0.289 0.270 0.287 0.329 0.292 0.301 0.274 0.277 0.327 0.299 0.320 0.299 0.275 0.408 0.326 0.259 SPILLED 0.296 0.295 0.325 0.303 0.291 0.282 0.278 0.346 0.316 0.374 0.294 0.283 0.312 0.337 0.272 0.250 0.286 0.301 0.268 SPILLED 0.267 0.341 0.296 0.289 0.291 0.278 0.254 0.388 0.277 0.251 0.241 0.263 0.294 0.297 0.286 0.281 0.258 0.279 SPILLED 0.289 0.336 0.271 0.254 0.248 0.323 0.241 0.242 0.263 0.272 0.254 SPILLED 0.255 SPILLED 0.270 0.264 0.261 0.251 0.262 0.278 0.285 0.247 SPILLED 0.234 0.260 0.275 0.260 0.248 0.242 0.252 0.410 0.286 0.273 0.244 0.267 0.256 SPILLED 0.243 0.235 0.248 0.245 0.233 SPILLED 0.256 SPILLED SPILLED 0.234 0.245 NOT TAKEN 0.259 SPILLED 0.202 0.216 SPILLED 0.283 0.237 NOT TAKEN SPILLED 0.262 0.265 0.235 SPILLED 0.254 NOT TAKEN 0.237 0.242 0.231 SPILLED SPILLED SPILLED 0.224 0.243 0.215 SPILLED 0.235 0.262 0.301 0.221 0.233 NOT TAKEN 0.252 0.274 0.220 0.229 SPILLED 0.249 0.223 NOT TAKEN 0.220 0.212 0.240 0.226 SPILLED 0.248 NOT TAKEN 0.221 0.230 0.224 SPILLED 0.306 0.212 0.219 0.234 0.224 0.270 0.230 SPILLED SPILLED 0.217 0.266 NOT TAKEN 0.232 0.249 0.217 0.225 0.205 0.226 0.208 NOT TAKEN 0.236 0.213 0.238 0.218 SPILLED 0.302 NOT TAKEN 0.225 0.228 0.217 0.245 0.209 0.205 0.221 0.219 0.206 SPILLED 0.241 0.256 SPILLED 0.188 SPILLED NOT TAKEN 0.223 0.258 0.216 0.220 0.222 0.216 0.192 NOT TAKEN 0.195 0.215 0.223 0.193 SPILLED SPILLED NOT TAKEN 0.224 0.221 0.222 SPILLED 0.196 0.195 0.209 0.217 0.213 SPILLED 0.218 0.317 0.329 0.194 0.228 NOT TAKEN 0.229 SPILLED 0.162 0.217 0.195 0.222 0.200 NOT TAKEN 0.194 0.211 0.232 0.191 SPILLED 0.229 NOT TAKEN 0.195 0.206 0.206 SPILLED 0.190 0.175 0.197 0.197 0.200 SPILLED 0.202 0.285 SPILLED 0.175 0.213 NOT TAKEN 0.203 0.221 0.174 0.205 0.176 0.200 0.178 NOT TAKEN 0.181 0.198 0.196 0.193 0.313 0.193 NOT TAKEN 0.190 0.185 0.198 SPILLED 0.184 0.181 0.194 0.188 0.188 SPILLED 0.200 SPILLED SPILLED 0.174 0.195 NOT TAKEN 0.201 0.221 0.196 0.193 0.175 0.192 0.170 NOT TAKEN 0.179 0.186 0.184 0.175 SPILLED 0.199 NOT TAKEN 0.185 0.187 0.183 1396 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 14 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 2 - 3 PPM C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 C91249 C91250 0.300 SPILLED 0.320 0.287 0.344 0.394 0.307 0.289 0.313 0.308 0.274 0.371 0.330 0.271 0.342 0.332 0.296 0.266 SPILLED 0.274 GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 2.525 2.988 2.816 3.093 2.807 3.358 2.507 2.980 3.960 2.822 2.796 SPILLED 3.053 3.009 2.903 3.167 3.068 3.057 2.301 2.709 2.783 2.938 2.777 2.608 2.487 2.907 SPILLED 2.651 2.542 2.452 2.703 2.772 2.605 2.885 2.776 SPILLED 0.247 SPILLED 0.311 0.258 0.341 SPILLED SPILLED 0.238 0.259 SPILLED 2.263 2.503 2.319 2.679 2.632 SPILLED 2.700 2.583 2.561 2.371 SPILLED 2.260 2.642 SPILLED 2.318 2.650 2.571 2.403 0.252 0.384 SPILLED 0.239 0.369 SPILLED 0.260 0.233 0.336 0.237 NOT TAKEN 2.391 2.536 2.555 3.030 2.573 SPILLED 2.234 2.522 2.273 2.688 2.400 2.446 2.500 2.778 2.692 2.512 2.458 0.233 SPILLED 0.271 0.223 0.256 SPILLED 0.263 0.218 0.252 0.238 NOT TAKEN 2.387 2.336 2.571 2.605 2.521 2.035 2.391 2.277 2.275 2.359 2.060 2.258 2.329 3.310 2.546 2.312 2.309 SPILLED SPILLED SPILLED 0.228 0.295 SPILLED 0.271 0.209 SPILLED SPILLED NOT TAKEN 2.236 2.315 2.468 2.830 2.242 2.067 2.261 2.160 2.194 2.355 1.987 2.177 2.201 2.130 2.571 2.262 2.129 0.216 SPILLED 0.227 0.231 SPILLED SPILLED 0.270 0.217 0.230 0.213 NOT TAKEN 2.115 2.002 2.313 2.463 2.118 1.833 2.008 2.105 2.028 2.298 1.909 1.969 2.027 2.176 2.143 2.067 1.919 0.312 0.300 SPILLED 0.213 0.258 SPILLED 0.292 0.202 0.229 0.321 NOT TAKEN 2.323 2.275 2.325 2.535 2.172 1.987 2.055 2.224 1.717 2.488 2.309 2.078 2.043 2.050 2.071 2.128 1.871 0.188 SPILLED 0.240 0.177 0.271 SPILLED SPILLED 0.176 0.199 0.188 NOT TAKEN 2.129 1.964 2.003 2.374 2.068 1.681 1.980 2.052 1.895 2.199 1.825 2.065 1.964 1.850 2.049 1.939 1.866 SPILLED SPILLED 0.271 0.189 SPILLED SPILLED SPILLED 0.185 0.201 0.276 NOT TAKEN 1.944 2.019 2.121 2.257 1.818 SPILLED 1.886 2.008 1.987 1.751 1.695 1.889 1.815 SPILLED 1.888 1.815 1.811 1397 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 15 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 3 - 30 PPM C91269 C91270 C91271 C91272 C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 2.973 3.514 3.052 3.150 SPILLED 2.781 2.976 2.785 2.930 2.896 3.411 3.176 2.799 3.190 3.188 3.227 2.542 2.637 4.219 2.574 SPILLED 2.774 3.136 2.841 3.522 3.183 3.056 2.608 4.201 2.924 SPILLED 2.796 3.012 SPILLED 2.911 3.565 2.674 2.921 2.626 2.643 SPILLED 3.617 2.835 2.821 2.857 2.872 2.965 2.297 2.474 2.634 2.350 2.945 2.434 2.614 2.647 4.044 2.864 3.433 SPILLED SPILLED 2.523 SPILLED 2.399 2.888 2.555 2.383 SPILLED 2.359 2.517 2.558 2.434 SPILLED SPILLED 2.628 SPILLED 2.807 2.708 2.825 2.071 2.452 3.033 2.179 SPILLED 2.346 2.432 2.556 3.111 2.571 3.688 SPILLED SPILLED 2.297 2.588 2.459 2.669 SPILLED NOT TAKEN 3.659 SPILLED 2.482 2.398 2.128 2.550 2.586 2.460 SPILLED 2.662 2.465 2.729 NOT TAKEN 2.650 NOT TAKEN 2.036 2.449 2.322 NOT TAKEN 2.618 SPILLED 2.545 SPILLED 2.580 2.441 2.429 2.547 2.440 2.551 3.472 NOT TAKEN 2.889 SPILLED 2.620 2.208 2.366 2.488 2.335 2.343 SPILLED 2.700 2.426 2.538 NOT TAKEN 2.508 NOT TAKEN 2.052 2.382 2.042 NOT TAKEN 2.214 SPILLED 2.341 2.870 2.262 2.621 2.369 2.365 2.119 2.468 2.831 NOT TAKEN SPILLED SPILLED 2.219 2.211 2.286 SPILLED 2.445 2.143 SPILLED 2.571 2.461 2.383 NOT TAKEN SPILLED NOT TAKEN 1.830 2.220 2.022 NOT TAKEN 2.039 SPILLED 2.100 SPILLED 2.262 2.680 2.123 2.403 2.186 2.288 SPILLED NOT TAKEN SPILLED 2.120 2.193 2.269 2.090 2.151 2.282 2.155 SPILLED 2.358 2.135 2.118 NOT TAKEN 2.921 NOT TAKEN 1.746 2.076 2.184 NOT TAKEN 2.010 SPILLED 2.080 2.400 2.167 2.719 1.979 2.163 SPILLED 2.268 SPILLED NOT TAKEN 2.525 SPILLED SPILLED 2.111 1.993 SPILLED 2.010 2.070 SPILLED 2.182 1.940 SPILLED NOT TAKEN 2.284 NOT TAKEN 1.762 2.151 2.018 NOT TAKEN 2.088 SPILLED SPILLED 3.819 2.355 2.188 2.036 2.128 2.092 2.078 2.143 NOT TAKEN SPILLED SPILLED SPILLED 2.016 1.788 1.795 1.939 2.111 SPILLED 2.184 2.057 2.112 NOT TAKEN 2.250 NOT TAKEN 1.708 1.843 1.915 NOT TAKEN 1.815 1.901 1.908 2.386 SPILLED SPILLED 1.967 1.920 1.959 1.997 SPILLED NOT TAKEN 2.345 2.526 2.211 1.967 1.837 2.085 2.037 1.942 SPILLED 2.025 1.993 1.952 NOT TAKEN 2.202 NOT TAKEN 1.599 1.937 1.733 NOT TAKEN 1.791 SPILLED 1.960 2.155 2.053 3.000 1.864 2.054 1398 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 16 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 3 - 30 PPM C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 4.247 3.110 SPILLED 2.966 3.264 3.069 SPILLED 2.842 3.051 3.010 3.027 SPILLED 2.720 SPILLED 2.788 2.877 2.777 SPILLED 2.757 2.850 2.772 2.760 GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 9.075 8.684 10.671 9.784 10.365 SPILLED 9.632 11.044 11.913 9.928 10.174 9.824 9.605 10.074 8.376 10.141 9.726 10.540 8.337 11.006 9.785 SPILLED SPILLED 8.693 10.967 10.661 SPILLED 10.106 9.574 8.386 8.909 7.431 8.489 8.627 3.823 2.710 SPILLED 2.571 2.593 2.525 2.857 2.454 2.741 2.594 2.420 SPILLED 7.351 SPILLED 9.134 9.315 9.335 7.961 9.746 8.914 SPILLED 9.005 SPILLED SPILLED 8.143 7.295 9.467 8.070 SPILLED 2.449 SPILLED 2.469 2.504 2.742 SPILLED 2.584 2.673 2.412 2.542 9.705 7.614 8.513 8.850 8.355 NOT TAKEN NOT TAKEN SPILLED 9.501 9.259 11.894 9.012 7.822 8.317 7.143 9.027 7.774 SPILLED 2.352 SPILLED 2.204 2.503 2.660 2.293 2.237 2.522 2.437 2.609 SPILLED 7.038 SPILLED SPILLED 8.122 NOT TAKEN NOT TAKEN 6.897 8.430 7.992 8.442 8.307 SPILLED 7.379 6.342 8.153 8.682 3.436 2.299 SPILLED 2.104 SPILLED 2.349 SPILLED 2.271 2.665 2.282 2.235 10.039 6.604 SPILLED SPILLED 8.304 NOT TAKEN NOT TAKEN 6.469 8.207 7.021 SPILLED 8.443 8.436 7.235 6.336 7.766 7.080 2.818 2.147 2.207 2.008 2.309 2.325 SPILLED 1.968 2.553 2.275 2.249 7.243 6.563 7.099 SPILLED 8.163 NOT TAKEN NOT TAKEN 6.329 7.337 6.823 7.478 7.317 SPILLED 7.281 6.143 8.173 7.049 3.680 2.220 SPILLED 1.826 2.380 2.360 SPILLED 2.490 2.621 2.058 2.202 7.685 6.769 8.503 SPILLED 7.995 NOT TAKEN NOT TAKEN 6.369 7.158 6.701 SPILLED 7.649 7.259 7.278 6.167 7.412 7.143 SPILLED 2.053 1.795 1.695 2.005 2.101 1.972 1.802 2.832 1.965 2.280 SPILLED 6.608 7.296 SPILLED 7.729 NOT TAKEN NOT TAKEN 5.957 6.899 6.389 7.061 7.336 6.665 6.620 5.992 6.730 6.553 SPILLED 2.095 SPILLED 1.871 2.038 2.136 1.915 1.735 SPILLED 1.949 2.017 6.682 6.198 SPILLED SPILLED 7.603 NOT TAKEN NOT TAKEN 5.426 6.789 6.467 6.676 6.681 6.501 6.151 5.419 6.671 6.531 1399 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 17 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 4 - 100 PPM C91328 C91329 C91330 C91331 C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 10.561 10.488 9.524 10.832 SPILLED 9.324 10.813 9.846 SPILLED 9.030 14.148 9.945 10.695 9.680 10.016 10.405 8.571 SPILLED SPILLED SPILLED 10.428 SPILLED SPILLED 9.003 9.744 9.909 11.379 11.264 10.724 8.946 6.524 9.228 9.470 8.517 9.140 9.654 9.159 9.422 9.073 SPILLED 7.677 SPILLED 8.656 9.882 8.378 8.204 9.423 7.705 8.665 10.330 SPILLED 8.990 8.929 SPILLED 8.781 8.295 9.197 SPILLED 11.251 9.689 7.979 8.980 7.849 8.740 8.265 9.363 9.594 SPILLED 8.565 8.601 SPILLED 7.081 10.851 8.571 8.947 8.388 7.291 7.862 7.255 7.877 8.506 SPILLED 8.383 8.096 SPILLED 7.499 8.043 7.836 SPILLED 9.104 8.969 7.857 8.022 8.163 9.069 7.877 8.571 8.658 NOT TAKEN 7.734 7.587 SPILLED 7.634 9.790 8.350 SPILLED 7.903 7.222 7.963 7.189 NOT TAKEN 8.414 SPILLED 7.976 7.816 NOT TAKEN 7.436 7.578 7.966 SPILLED 9.432 9.856 7.199 7.792 7.300 7.567 6.996 8.349 8.411 NOT TAKEN 7.067 7.192 SPILLED 6.853 7.212 7.206 8.163 7.157 7.586 7.734 6.674 NOT TAKEN 7.657 7.038 7.143 7.975 NOT TAKEN 6.975 7.075 7.615 SPILLED 8.237 SPILLED 6.752 7.308 6.701 7.284 7.334 7.744 7.424 NOT TAKEN 7.012 7.542 SPILLED 6.757 7.412 7.338 7.407 6.906 6.775 7.787 6.252 NOT TAKEN 7.226 6.892 7.127 7.023 NOT TAKEN 7.566 7.338 6.832 SPILLED 7.561 7.418 6.458 6.719 6.507 6.975 6.617 7.255 6.973 NOT TAKEN 6.529 6.973 SPILLED 6.262 7.358 6.494 6.676 6.415 7.128 6.980 6.611 NOT TAKEN 7.210 6.647 6.820 6.643 NOT TAKEN 6.699 6.690 7.437 6.149 7.430 7.279 6.449 6.748 6.554 6.905 6.598 7.280 8.905 NOT TAKEN 6.349 6.932 SPILLED 6.634 6.746 7.114 6.478 6.367 7.057 6.706 6.103 NOT TAKEN 6.859 6.756 6.105 7.085 NOT TAKEN 6.559 6.168 6.809 6.389 7.654 SPILLED 6.267 6.798 6.275 6.349 6.434 6.710 7.735 NOT TAKEN 5.802 6.862 16.507 5.887 5.781 6.130 6.061 5.982 7.058 6.767 6.166 NOT TAKEN 6.429 6.202 6.070 7.537 NOT TAKEN 6.526 5.950 6.709 5.750 7.664 6.418 5.871 6.984 6.001 6.184 6.196 6.958 6.466 NOT TAKEN 5.975 6.531 10.286 6.094 6.609 6.059 5.810 5.940 6.392 6.478 6.064 NOT TAKEN 6.489 6.038 6.477 6.185 NOT TAKEN 6.095 5.802 6.621 5.893 7.630 6.130 5.559 6.637 1400 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 18 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 4 - 100 PPM C91359 C91360 C91361 C91362 C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 11.650 10.494 SPILLED 10.767 10.824 10.714 SPILLED 9.952 10.884 11.052 9.552 11.285 8.804 12.281 9.996 SPILLED 10.799 9.923 10.141 9.470 10.381 10.019 SPILLED 10.382 8.780 12.662 9.852 10.504 10.870 8.079 9.353 11.843 8.702 10.766 8.399 9.125 8.962 SPILLED 9.098 8.618 9.159 8.586 13.551 8.837 GROUP: FEMALE 5 - 300 PPM C91381 C91382 C91383 C91384 C91385 C91386 SPILLED 25.325 27.763 42.857 SPILLED 29.196 23.851 25.850 24.926 25.835 26.925 SPILLED 9.219 SPILLED 7.602 11.638 8.940 8.856 SPILLED 8.399 8.372 SPILLED 8.092 9.115 7.623 10.218 7.799 8.172 8.302 7.838 8.621 7.761 8.864 8.450 22.067 19.540 23.506 21.601 22.061 SPILLED 8.466 8.112 7.367 SPILLED 8.618 8.429 SPILLED 8.010 7.198 10.575 7.774 8.718 7.246 8.484 8.084 SPILLED 8.630 7.293 8.725 7.645 8.865 7.714 23.822 21.617 23.129 25.919 23.920 SPILLED 7.798 7.074 6.784 SPILLED 7.129 7.427 SPILLED 7.143 7.237 9.039 8.098 8.479 7.254 8.595 7.390 7.326 7.685 7.351 7.954 7.640 8.682 7.403 21.697 19.951 21.537 SPILLED 23.044 SPILLED 7.891 7.360 6.641 SPILLED 7.671 6.965 8.163 7.615 7.417 7.281 7.611 7.861 6.764 6.883 7.079 6.908 7.188 6.911 7.489 7.234 7.957 6.954 20.911 21.182 21.376 22.489 30.537 SPILLED 8.584 6.865 6.029 8.453 7.143 6.095 7.728 6.862 6.292 7.024 7.067 7.259 SPILLED 6.773 6.875 5.921 6.593 6.455 7.627 7.009 7.051 6.800 19.563 18.940 18.578 SPILLED 24.965 SPILLED SPILLED 6.842 5.209 SPILLED 7.247 6.294 9.201 6.874 7.130 6.647 6.848 7.536 SPILLED SPILLED 6.712 6.142 6.808 6.580 7.490 6.478 6.645 6.774 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 6.531 6.009 6.209 6.470 5.811 SPILLED 6.209 5.804 5.819 6.759 7.351 SPILLED 6.087 6.520 6.126 6.803 5.991 6.925 5.933 7.053 6.500 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 6.877 6.026 SPILLED 6.218 5.822 SPILLED 5.450 5.979 5.864 6.627 6.803 8.821 6.379 6.009 5.527 6.202 5.969 6.545 6.272 7.026 6.981 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1401 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 19 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 5 - 3 0 0 PPM C91387 C91388 C91389 C91390 C91391 C91392 C91393 C91394 C91395 C91396 C91397 C91398 C91399 C91400 C91401 C91402 C91403 C91404 C91405 C91406 C91407 C91408 C91409 C91410 C91411 C91412 C91413 C91414 C91415 C91416 C91417 25.075 27.940 29.132 29.972 27.479 SPILLED 24.409 25.761 36.029 SPILLED 33.653 29.432 27.857 28.496 30.286 26.025 SPILLED 25.907 28.042 27.318 29.732 27.786 SPILLED SPILLED 30.521 28.992 SPILLED 30.062 25.476 24.333 30.938 20.420 25.038 19.821 25.661 23.953 26.429 41.409 24.164 31.906 SPILLED 25.356 22.632 22.842 24.154 32.666 22.785 34.689 22.651 24.561 24.176 23.795 25.108 20.513 29.748 26.356 23.160 SPILLED 26.698 21.548 23.489 SPILLED 18.420 22.500 19.540 25.739 21.652 SPILLED SPILLED 21.153 42.730 19.826 24.196 23.294 20.029 25.127 23.240 21.951 30.064 21.730 22.257 21.138 23.903 22.192 19.524 21.310 22.534 22.568 SPILLED 22.799 22.309 19.525 24.330 18.182 22.199 20.733 24.922 21.429 20.526 19.927 20.346 24.735 21.574 24.039 21.534 18.932 25.676 22.231 22.120 SPILLED 21.831 22.047 SPILLED 21.659 SPILLED 17.946 19.303 21.277 19.348 SPILLED 21.481 18.033 18.543 23.713 23.209 20.285 18.299 22.991 19.275 22.238 18.016 19.167 SPILLED 17.945 23.461 19.952 18.367 24.570 20.698 20.269 SPILLED 20.377 22.127 20.324 21.373 21.709 19.833 19.073 20.145 20.879 21.889 22.963 19.955 19.068 20.295 17.914 19.266 19.022 24.238 18.642 19.733 17.623 18.624 22.164 18.273 22.709 20.168 18.845 24.437 20.465 20.639 SPILLED 20.786 22.053 19.665 SPILLED 19.414 17.582 18.614 20.045 20.339 SPILLED 21.334 18.634 19.027 19.403 17.680 19.929 19.728 23.085 18.781 20.227 18.477 18.002 22.164 17.433 20.580 18.849 19.113 23.041 20.000 20.533 SPILLED 19.395 21.176 19.395 20.596 19.126 18.431 18.729 18.182 17.840 18.522 19.027 18.950 17.424 19.395 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1402 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 20 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 5 - 3 0 0 PPM C91418 C91419 C91420 C91421 C91422 C91423 C91424 C91425 C91426 C91427 C91428 C91429 C91430 C91431 C91432 C91433 C91434 C91435 C91436 C91437 C91438 C91439 C91440 C91441 C91442 C91443 C91444 C91445 C91446 C91447 C91448 25.835 SPILLED 29.630 26.804 SPILLED 25.051 25.815 SPILLED 30.777 29.435 30.192 27.862 29.227 28.220 21.599 39.110 28.398 28.083 23.003 26.802 31.504 33.236 SPILLED 30.000 28.656 25.691 30.458 SPILLED 32.180 30.959 29.011 24.290 SPILLED 32.688 24.568 20.665 25.237 24.702 22.135 38.884 SPILLED 25.786 22.870 26.802 22.414 22.843 34.421 25.051 25.876 24.339 24.390 27.607 26.037 25.893 25.032 24.213 21.542 24.852 25.210 27.706 24.697 SPILLED 23.785 22.206 23.233 21.237 19.694 23.915 21.486 19.676 23.174 23.100 25.355 19.515 24.702 20.714 19.328 SPILLED 22.432 24.242 21.800 21.861 24.773 22.637 22.388 21.188 23.209 21.641 24.010 SPILLED 24.529 22.350 SPILLED 21.866 SPILLED 22.294 19.514 17.143 NOT TAKEN 19.831 17.811 17.197 NOT TAKEN 22.857 19.565 NOT TAKEN SPILLED 21.275 SPILLED 21.884 23.433 20.351 21.581 24.122 SPILLED 21.792 21.943 22.148 NOT TAKEN 23.068 20.251 23.957 20.589 NOT TAKEN 21.853 19.780 19.748 20.375 15.761 NOT TAKEN 19.500 18.701 21.429 NOT TAKEN 21.932 19.988 NOT TAKEN SPILLED 20.887 SPILLED 20.977 21.922 18.267 20.455 23.336 20.168 19.811 20.991 20.779 NOT TAKEN 21.700 20.100 24.454 21.320 NOT TAKEN 19.748 18.792 19.527 18.653 16.459 NOT TAKEN 19.462 17.401 25.663 NOT TAKEN 20.203 18.279 NOT TAKEN SPILLED 19.222 18.169 19.398 21.188 21.257 19.868 22.091 20.121 19.580 20.387 21.481 NOT TAKEN 20.896 SPILLED 22.619 19.683 NOT TAKEN 19.162 18.883 18.960 18.737 16.396 NOT TAKEN 18.440 17.430 19.951 NOT TAKEN 20.750 17.073 NOT TAKEN SPILLED 20.111 19.764 19.121 21.287 19.797 20.157 21.590 17.832 20.417 19.845 19.208 NOT TAKEN 19.748 19.216 22.796 25.714 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1403 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 21 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 5 - 300 PPM C91449 C91450 28.410 24.838 29.635 22.619 GROUP: FEMALE 6 - 100 PPM C91451 C91452 C91453 C91454 C91455 C91456 C91457 C91458 C91459 C91460 C91461 C91462 C91463 C91464 C91465 C91466 C91467 C91468 C91469 C91470 C91471 C91472 C91473 C91474 C91475 C91476 9.055 9.416 11.003 10.282 10.703 10.671 10.909 9.321 9.716 9.460 8.668 10.834 SPILLED 9.958 10.919 9.301 10.367 8.530 9.049 10.417 10.244 9.905 9.439 11.077 13.870 SPILLED 8.427 8.498 9.577 8.891 12.166 8.516 9.624 8.041 9.870 9.041 8.369 9.472 8.269 8.965 SPILLED 7.852 11.018 7.750 8.055 9.967 8.696 8.242 8.019 10.352 11.136 8.665 SPILLED 21.772 7.526 7.798 8.429 8.011 10.507 7.678 7.872 8.442 7.579 7.988 7.870 8.954 7.048 8.457 9.704 8.019 7.600 7.833 7.906 8.727 8.271 8.880 7.740 8.889 10.151 7.854 SPILLED 19.023 7.875 7.758 8.605 7.861 8.212 7.741 8.008 7.212 7.965 7.546 7.565 8.317 6.806 8.184 8.498 7.787 8.403 7.362 7.077 8.016 7.889 9.377 7.420 8.910 SPILLED 7.961 36.882 SPILLED SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 15.345 17.009 15.824 NOT TAKEN NOT TAKEN NOT TAKEN 7.937 7.366 8.108 7.373 7.834 7.212 7.770 7.471 7.727 7.362 7.292 8.059 6.775 7.929 8.578 6.745 7.434 7.231 6.934 8.179 7.914 9.464 7.410 6.930 8.239 7.509 7.191 6.624 7.558 6.643 7.448 6.777 7.519 6.745 SPILLED 6.926 6.956 7.701 5.864 7.094 8.598 5.832 8.367 7.042 6.570 7.849 7.481 7.418 6.775 7.852 8.760 7.026 6.782 7.044 7.158 6.752 7.302 6.356 6.821 6.520 6.004 6.117 6.162 7.615 6.223 6.667 7.094 6.472 7.128 6.152 6.276 6.491 7.000 7.072 7.393 7.236 10.430 6.776 8.608 7.170 7.483 6.369 8.092 6.482 7.323 6.909 SPILLED 6.477 6.732 7.545 5.725 6.842 8.312 6.247 SPILLED 7.718 6.401 7.842 6.835 SPILLED SPILLED 8.437 SPILLED 6.657 6.222 6.268 6.667 5.799 7.008 6.162 6.790 6.071 6.589 6.148 6.038 6.902 5.571 6.398 6.623 6.200 6.272 5.941 6.027 6.836 6.754 SPILLED 6.555 7.268 6.920 6.361 6.589 6.714 6.726 6.389 6.850 6.471 6.498 6.030 SPILLED 7.397 5.961 6.732 5.378 6.559 6.708 5.558 6.483 7.508 6.012 7.046 6.674 7.290 7.809 SPILLED SPILLED 6.469 1404 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 22 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 6 - 100 PPM C91477 C91478 C91479 C91480 C91481 C91482 C91483 C91484 C91485 C91486 C91487 C91488 C91489 C91490 8.891 SPILLED 12.841 10.149 10.492 10.268 11.632 8.571 9.668 9.288 11.276 NOT TAKEN 8.496 10.174 8.041 10.396 11.287 8.833 9.467 9.470 9.085 7.933 9.102 8.102 8.809 9.058 8.108 9.473 GROUP: FEMALE 7 - 300 PPM C91491 C91492 C91493 C91494 C91495 C91496 C91497 C91498 C91499 C91500 C91501 C91502 C91503 C91504 29.105 SPILLED SPILLED 27.956 29.250 31.144 28.325 27.285 28.571 26.859 24.457 29.845 29.602 SPILLED 27.016 41.362 26.120 23.422 24.048 24.917 24.654 31.334 28.992 23.417 20.086 24.943 26.708 SPILLED 7.746 9.278 9.242 8.304 8.933 9.233 10.190 7.451 8.318 7.841 7.870 8.120 8.204 8.579 22.390 23.861 SPILLED 20.657 20.752 21.193 22.022 20.888 21.309 19.748 21.654 23.786 21.625 SPILLED SPILLED 8.103 9.003 7.615 8.163 8.823 SPILLED 7.127 7.651 7.712 7.326 SPILLED 8.231 SPILLED 22.294 SPILLED 21.661 20.856 20.324 21.081 21.223 22.067 19.763 19.233 18.927 22.093 21.333 SPILLED 7.339 7.565 8.417 7.094 7.583 8.026 8.778 7.128 7.323 6.963 7.079 8.583 7.175 7.926 20.368 22.771 20.207 19.319 20.450 20.922 20.532 21.255 18.189 17.143 19.334 20.833 20.702 SPILLED 6.794 6.918 7.611 6.782 7.159 7.578 6.514 6.678 7.254 6.427 6.418 6.729 6.847 7.174 19.443 21.012 18.169 17.875 18.383 18.805 19.498 26.203 17.594 17.102 SPILLED 19.349 18.418 35.588 7.220 6.797 7.337 6.551 6.574 7.219 7.426 6.064 6.446 6.394 6.282 6.396 6.694 7.127 18.455 19.986 18.697 18.929 20.229 19.190 19.576 SPILLED 17.811 16.405 17.184 19.956 20.241 25.545 6.814 SPILLED 8.015 6.257 6.425 7.011 SPILLED 6.642 7.008 6.050 6.630 6.390 7.005 7.098 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 6.480 7.345 7.370 6.160 6.424 6.773 6.325 6.126 6.148 5.998 5.919 6.264 6.895 7.231 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 6.997 6.772 6.513 6.336 6.869 6.558 5.714 5.848 6.219 5.747 5.851 6.015 6.266 6.491 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1405 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 23 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 7 - 3 0 0 PPM C91505 C91506 C91507 C91508 C91509 C91510 C91511 C91512 C91513 C91514 C91515 C91516 C91517 C91518 C91519 C91520 C91521 C91522 C91523 C91524 C91525 C91526 C91527 C91528 C91529 C91530 28.052 28.652 33.791 SPILLED 28.654 28.325 28.323 25.268 27.318 30.751 SPILLED 28.312 28.094 29.086 25.352 28.391 28.656 37.927 31.504 SPILLED 42.110 26.959 28.875 31.366 SPILLED 27.731 23.823 25.668 28.739 32.366 26.057 25.580 23.249 24.290 25.854 26.786 SPILLED 23.553 SPILLED 26.625 SPILLED 24.060 24.286 31.148 35.584 23.396 25.087 22.785 SPILLED 24.866 28.791 23.718 22.120 22.625 25.522 24.310 20.244 21.951 SPILLED 20.659 20.773 24.733 25.183 20.856 20.183 20.502 SPILLED 22.556 21.596 24.170 34.138 20.866 22.709 21.376 22.761 23.003 27.198 20.260 20.705 21.064 35.159 24.228 SPILLED 21.578 21.159 19.598 20.007 22.981 20.982 21.594 19.894 20.654 SPILLED 22.164 19.000 22.568 23.525 19.310 21.700 21.377 20.540 21.167 24.070 21.482 20.727 21.279 23.297 SPILLED SPILLED 19.262 21.012 19.780 18.956 21.728 20.346 19.979 21.128 19.887 SPILLED 20.890 21.271 26.571 25.131 21.864 20.844 20.466 SPILLED 19.048 20.472 18.606 SPILLED 19.929 22.522 SPILLED 19.160 18.609 20.810 18.455 18.176 20.316 22.523 20.119 19.022 19.048 SPILLED 19.669 19.388 20.786 22.785 20.045 20.131 19.841 21.239 21.114 21.096 18.598 18.310 19.515 21.266 SPILLED 17.992 19.666 20.408 18.066 17.225 20.709 20.061 18.634 18.336 20.069 40.378 21.024 17.184 20.143 21.951 21.096 19.749 19.691 19.963 18.526 19.506 18.480 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1406 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 24 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 0.149 0.176 0.155 0.150 0.147 0.137 0.152 0.141 NOT TAKEN 0.152 0.155 0.144 0.146 0.136 0.152 0.166 0.138 0.141 0.142 0.135 0.152 0.137 0.141 0.138 0.144 0.150 0.132 0.149 0.147 0.147 0.150 0.144 0.146 0.139 0.142 0.147 0.135 0.147 0.138 NOT TAKEN 0.149 0.170 0.139 0.142 0.140 0.158 SPILLED 0.134 0.136 0.141 0.132 0.149 0.136 0.140 0.133 0.139 0.154 0.134 0.117 0.150 0.139 0.147 0.141 0.149 0.141 0.149 0.150 0.141 0.140 0.137 NOT TAKEN 0.144 0.150 0.142 0.137 0.147 0.152 0.189 0.132 0.137 0.139 0.138 0.152 0.141 0.138 0.134 0.147 0.148 0.136 SPILLED 0.149 0.144 0.145 0.140 0.146 0.138 0.147 0.149 0.141 0.138 0.132 NOT TAKEN 0.137 0.133 0.140 0.133 0.143 0.142 0.174 NOT TAKEN 0.139 0.137 0.127 0.146 0.130 0.137 0.130 0.145 0.147 NOT TAKEN 0.141 0.144 0.139 0.144 0.135 0.142 0.143 0.139 0.148 0.139 0.138 0.135 NOT TAKEN 0.142 0.158 0.120 0.141 0.142 0.142 0.158 NOT TAKEN 0.135 0.137 0.130 0.148 0.140 0.141 0.135 0.144 0.152 NOT TAKEN 0.138 0.140 0.136 0.139 0.125 0.140 0.140 0.140 0.138 0.124 0.133 0.135 NOT TAKEN 0.137 0.149 0.110 0.136 0.148 0.135 0.149 NOT TAKEN 0.132 0.141 0.129 0.139 0.141 0.135 0.124 0.144 0.152 NOT TAKEN 0.136 0.143 0.135 0.139 0.124 0.130 0.136 0.142 0.139 0.134 0.137 0.128 NOT TAKEN 0.143 0.148 0.144 0.126 0.138 0.128 0.141 NOT TAKEN 0.140 0.139 0.126 0.139 0.133 0.128 0.125 0.136 0.151 NOT TAKEN 0.130 0.141 0.129 0.134 0.120 0.126 0.134 0.132 0.132 0.127 0.120 0.121 NOT TAKEN 0.124 0.138 0.110 0.120 0.124 0.120 0.138 NOT TAKEN 0.130 0.126 0.121 0.133 0.130 0.120 0.116 0.130 0.147 NOT TAKEN 0.125 0.132 0.127 0.131 0.113 0.071 0.128 0.132 0.124 0.126 0.118 0.120 NOT TAKEN 0.118 0.129 0.098 0.117 0.067 0.116 0.131 NOT TAKEN 0.123 0.115 0.115 0.116 0.126 0.117 0.120 0.123 0.156 NOT TAKEN 0.114 0.127 0.104 0.121 0.114 0.120 0.130 0.132 0.124 0.133 0.121 0.118 NOT TAKEN 0.130 0.140 0.126 0.119 0.022 0.121 0.135 NOT TAKEN 0.120 0.129 0.118 0.125 0.134 0.115 0.118 0.124 0.132 NOT TAKEN 0.123 0.127 0.114 0.117 1407 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 25 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 2 - 3 PPM C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 0.136 NOT TAKEN NOT TAKEN 0.144 0.148 NOT TAKEN 0.164 0.135 0.134 0.138 0.150 0.163 0.143 0.146 0.176 0.149 0.149 0.139 0.152 0.151 0.145 NOT TAKEN NOT TAKEN 0.160 0.154 0.147 0.146 0.144 0.143 0.132 NOT TAKEN NOT TAKEN 0.142 0.145 NOT TAKEN 0.154 0.126 0.130 0.134 0.140 0.154 0.132 0.134 0.162 0.137 0.146 0.128 0.140 0.144 0.136 NOT TAKEN NOT TAKEN 0.147 0.149 0.139 0.144 0.139 0.139 0.135 NOT TAKEN NOT TAKEN 0.139 0.141 NOT TAKEN 0.164 0.120 0.134 0.133 0.147 0.158 0.135 0.133 SPILLED 0.135 0.152 0.134 0.138 0.146 0.137 NOT TAKEN NOT TAKEN 0.140 0.149 0.144 0.143 0.138 0.133 0.133 NOT TAKEN NOT TAKEN 0.137 0.128 NOT TAKEN NOT TAKEN 0.122 0.110 0.132 0.150 0.156 0.140 0.135 SPILLED 0.132 0.154 0.137 0.139 NOT TAKEN 0.133 NOT TAKEN NOT TAKEN 0.138 NOT TAKEN 0.143 0.142 0.137 0.118 0.132 NOT TAKEN NOT TAKEN 0.138 0.140 NOT TAKEN NOT TAKEN 0.131 0.130 0.133 0.149 0.152 0.136 0.137 0.175 0.132 0.143 0.133 0.146 NOT TAKEN 0.135 NOT TAKEN NOT TAKEN 0.141 NOT TAKEN 0.135 0.147 0.130 0.140 0.134 NOT TAKEN NOT TAKEN 0.131 0.135 NOT TAKEN NOT TAKEN 0.133 0.126 0.132 0.146 0.149 0.132 0.127 0.167 0.133 0.148 0.133 0.138 NOT TAKEN 0.128 NOT TAKEN NOT TAKEN 0.144 NOT TAKEN 0.134 0.139 0.134 0.134 0.129 NOT TAKEN NOT TAKEN 0.131 0.130 NOT TAKEN NOT TAKEN 0.120 0.120 0.130 0.139 0.143 0.129 0.126 0.161 0.133 0.135 0.124 0.134 NOT TAKEN 0.126 NOT TAKEN NOT TAKEN 0.136 NOT TAKEN 0.131 0.135 0.135 0.129 0.120 NOT TAKEN NOT TAKEN 0.123 0.125 NOT TAKEN NOT TAKEN 0.121 0.112 0.119 0.133 0.130 0.124 0.115 0.148 0.122 0.128 0.125 0.126 NOT TAKEN 0.118 NOT TAKEN NOT TAKEN 0.127 NOT TAKEN 0.123 0.122 0.121 0.121 0.121 NOT TAKEN NOT TAKEN 0.114 0.121 NOT TAKEN NOT TAKEN 0.117 0.111 0.118 0.129 0.126 0.120 0.112 0.094 0.116 0.123 0.112 0.065 NOT TAKEN 0.115 NOT TAKEN NOT TAKEN 0.120 NOT TAKEN 0.117 0.070 0.080 0.110 0.125 NOT TAKEN NOT TAKEN 0.122 0.123 NOT TAKEN NOT TAKEN 0.118 0.111 0.121 0.135 0.120 0.117 0.120 SPILLED 0.120 0.118 0.115 0.125 NOT TAKEN 0.115 NOT TAKEN NOT TAKEN 0.124 NOT TAKEN 0.116 0.116 0.114 0.107 1408 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 26 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 3 - 3 0 PPM C90841 C90842 C90843 C90844 C90845 C90846 C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 1.554 1.476 1.465 SPILLED 1.446 NOT TAKEN 1.457 1.494 1.459 1.433 1.600 1.461 NOT TAKEN 1.527 1.383 1.443 1.429 1.502 1.547 1.501 1.526 1.633 1.310 1.414 1.349 NOT TAKEN 1.405 1.466 1.409 1.398 1.314 1.472 1.424 1.360 1.723 1.343 NOT TAKEN 1.366 1.511 1.385 1.364 1.427 1.466 NOT TAKEN 1.451 1.385 1.317 1.409 1.394 1.497 1.602 1.462 1.600 1.243 1.386 1.333 NOT TAKEN 1.375 1.417 1.313 1.369 1.304 1.415 1.440 1.351 1.673 1.325 NOT TAKEN 1.372 1.418 1.351 1.358 1.434 1.429 NOT TAKEN 1.496 1.441 1.342 1.429 1.408 1.540 1.601 1.487 1.619 1.268 1.431 1.351 NOT TAKEN 1.414 1.390 1.344 1.394 1.304 1.413 1.401 NOT TAKEN 1.709 1.365 NOT TAKEN 1.302 1.450 1.299 1.323 1.248 NOT TAKEN NOT TAKEN SPILLED 1.337 1.117 1.357 1.456 1.448 1.551 1.377 1.555 NOT TAKEN 1.354 1.315 NOT TAKEN 1.367 1.359 1.253 1.316 1.248 1.371 1.380 NOT TAKEN 1.683 1.330 NOT TAKEN 1.348 1.451 1.274 1.299 1.431 NOT TAKEN NOT TAKEN SPILLED 1.318 1.289 1.403 1.420 1.408 1.606 1.423 1.567 NOT TAKEN 1.385 1.343 NOT TAKEN 1.355 1.366 1.276 1.401 1.292 1.375 1.329 NOT TAKEN 1.786 1.292 NOT TAKEN 1.373 1.409 1.308 1.282 1.359 NOT TAKEN NOT TAKEN 1.494 1.311 1.283 1.402 1.449 1.389 1.536 1.453 1.562 NOT TAKEN 1.355 1.320 NOT TAKEN 1.341 1.369 1.315 1.204 1.265 1.355 1.378 NOT TAKEN 1.600 1.314 NOT TAKEN 1.397 1.337 1.238 1.282 1.406 NOT TAKEN NOT TAKEN SPILLED 1.278 1.378 1.392 1.379 1.428 1.573 1.402 1.545 NOT TAKEN 1.402 1.335 NOT TAKEN 1.319 1.335 1.278 1.306 1.245 1.367 1.309 NOT TAKEN 1.472 1.275 NOT TAKEN 1.329 1.235 1.214 1.234 1.312 NOT TAKEN NOT TAKEN 1.463 1.215 1.270 1.297 1.363 1.336 1.392 1.332 1.448 NOT TAKEN 1.301 1.293 NOT TAKEN 1.326 1.204 1.198 1.235 1.196 1.284 1.279 NOT TAKEN 0.704 1.165 NOT TAKEN 1.271 1.259 1.141 1.192 1.243 NOT TAKEN NOT TAKEN SPILLED 1.104 1.150 1.214 1.324 1.229 1.403 1.292 1.352 NOT TAKEN 1.212 1.253 NOT TAKEN 1.359 1.141 1.229 1.211 1.137 1.268 1.351 NOT TAKEN 1.412 1.134 NOT TAKEN 1.347 1.149 1.189 1.186 1.281 NOT TAKEN NOT TAKEN 1.467 1.196 1.235 1.214 1.326 1.438 1.456 1.293 1.385 NOT TAKEN 1.281 1.323 NOT TAKEN 1.355 1.166 1.234 1.232 1.205 1409 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 27 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 3 - 3 0 PPM C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 1.369 1.425 1.332 1.436 1.415 1.524 1.579 1.487 1.474 1.429 1.466 SPILLED 1.639 1.454 1.523 1.494 1.412 1.357 NOT TAKEN NOT TAKEN 1.573 1.647 1.543 1.713 SPILLED 1.397 1.465 1.519 1.454 1.340 1.322 1.337 1.409 1.300 1.495 1.598 1.419 1.399 1.395 1.374 1.710 1.558 1.373 1.415 1.380 1.399 1.272 NOT TAKEN NOT TAKEN 1.446 1.476 1.417 1.559 1.584 1.300 1.368 1.403 1.393 1.367 1.420 1.290 1.457 1.292 1.494 1.585 1.419 1.456 1.416 1.411 1.517 SPILLED 1.374 1.413 1.383 1.412 1.232 NOT TAKEN NOT TAKEN 1.452 1.455 1.422 1.654 1.653 1.328 1.326 1.513 1.414 1.388 1.446 1.215 1.370 1.209 NOT TAKEN 1.533 1.387 NOT TAKEN 1.366 1.396 SPILLED 1.519 1.367 1.379 1.402 0.688 1.239 NOT TAKEN NOT TAKEN 1.290 1.459 1.483 1.506 1.463 1.013 1.360 1.380 1.365 1.330 1.488 1.238 1.456 1.166 NOT TAKEN 1.506 1.376 NOT TAKEN 1.307 1.361 1.600 SPILLED 1.350 1.346 SPILLED 1.760 1.253 NOT TAKEN NOT TAKEN 1.482 1.441 1.407 1.487 SPILLED 1.351 1.292 1.459 1.392 1.357 1.332 1.239 1.378 1.136 NOT TAKEN 1.519 1.396 NOT TAKEN 1.345 1.376 1.559 SPILLED 1.351 1.364 1.413 1.544 1.321 NOT TAKEN NOT TAKEN 1.399 1.465 1.409 1.407 1.423 1.390 1.288 1.423 1.426 1.323 1.403 1.174 1.334 1.243 NOT TAKEN 1.523 1.359 NOT TAKEN 1.282 1.287 1.466 SPILLED 1.379 1.380 1.336 1.399 1.264 NOT TAKEN NOT TAKEN 1.372 1.439 1.348 1.364 1.514 1.334 1.378 1.394 1.398 1.289 1.295 1.136 1.265 1.200 NOT TAKEN 1.461 1.387 NOT TAKEN 1.280 1.299 1.494 1.597 1.239 1.259 1.296 1.268 1.215 NOT TAKEN NOT TAKEN 1.276 1.329 1.309 1.297 1.399 1.221 1.292 1.422 1.310 1.308 1.320 1.104 1.144 1.116 NOT TAKEN 1.413 1.263 NOT TAKEN 1.208 1.253 1.414 1.498 1.159 1.222 1.182 1.275 1.185 NOT TAKEN NOT TAKEN 1.247 1.263 1.209 1.231 1.301 1.117 1.180 1.278 1.263 1.198 1.269 1.082 1.201 1.086 NOT TAKEN 1.348 1.231 NOT TAKEN 1.163 1.223 1.332 1.454 1.201 1.289 1.254 1.350 1.273 NOT TAKEN NOT TAKEN 1.272 1.346 1.275 1.312 1.389 1.183 1.241 1.379 1.304 1410 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 28 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 4.794 4.715 4.746 4.863 5.119 5.017 4.632 4.641 4.821 5.065 4.980 4.531 5.262 SPILLED 4.885 4.816 4.660 4.762 4.682 4.675 4.653 4.584 4.905 5.190 4.984 4.521 4.606 4.478 4.877 5.216 4.659 4.757 4.660 4.538 4.492 5.057 4.836 4.361 4.666 4.696 4.689 4.617 4.380 4.709 SPILLED 4.727 4.594 4.581 4.731 4.613 4.559 4.528 4.491 4.673 4.934 4.855 4.538 4.492 4.405 4.643 5.239 4.391 4.695 4.767 4.589 4.703 5.046 4.721 4.542 4.722 4.594 4.906 4.836 4.496 4.551 4.853 4.617 4.648 4.587 4.732 4.482 4.632 4.364 4.625 4.623 4.915 4.803 4.329 4.565 4.612 3.509 5.072 4.537 4.855 4.644 4.429 4.577 NOT TAKEN 4.739 NOT TAKEN 4.613 4.570 4.752 4.803 4.374 5.064 4.611 4.796 4.711 NOT TAKEN 4.613 4.510 4.452 NOT TAKEN 4.589 4.649 4.875 4.827 4.444 4.481 4.535 5.079 4.942 4.482 4.970 4.613 4.526 4.507 NOT TAKEN 4.744 NOT TAKEN 4.762 4.474 4.846 4.771 4.281 4.847 4.529 4.783 4.757 NOT TAKEN 4.556 4.421 4.325 NOT TAKEN 4.468 4.646 4.534 4.836 4.413 4.472 4.499 5.137 4.917 4.424 4.787 4.541 4.627 4.422 NOT TAKEN 4.661 NOT TAKEN 4.573 4.441 4.721 4.673 4.282 5.030 4.465 4.660 4.619 NOT TAKEN 4.309 4.552 4.465 NOT TAKEN 4.513 4.531 4.597 4.781 4.516 4.454 4.619 4.916 4.766 4.402 4.936 4.520 4.378 4.480 NOT TAKEN 4.668 NOT TAKEN 4.505 4.764 4.756 4.738 4.624 5.143 4.582 4.401 4.590 NOT TAKEN 4.520 4.516 4.569 NOT TAKEN 4.622 4.673 4.681 4.682 4.399 4.392 4.508 4.666 4.489 4.419 4.589 4.268 4.310 4.521 NOT TAKEN 4.543 NOT TAKEN 4.423 4.470 4.572 4.440 4.237 4.774 4.373 4.377 4.499 NOT TAKEN 4.163 4.356 4.222 NOT TAKEN 4.360 4.387 4.220 4.411 4.170 4.326 4.376 4.391 4.521 4.266 4.527 4.183 4.030 4.170 NOT TAKEN 4.445 NOT TAKEN 4.293 4.000 4.284 4.259 3.670 4.687 4.128 4.461 4.530 NOT TAKEN 4.153 4.188 4.124 NOT TAKEN 4.225 4.440 4.181 4.589 3.964 4.038 4.102 4.485 4.473 4.100 4.522 4.201 3.898 4.232 NOT TAKEN 4.524 NOT TAKEN 3.992 3.919 4.283 4.263 4.283 4.357 4.101 4.241 4.487 NOT TAKEN 4.317 3.934 4.076 NOT TAKEN 4.217 4.636 3.786 4.472 4.207 5.270 4.130 4.547 4.424 4.237 1411 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 29 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 4 - 100 PPM C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 4.922 4.594 5.114 NOT TAKEN 5.040 4.747 5.192 NOT TAKEN 4.850 4.802 NOT TAKEN 5.442 4.668 4.725 4.020 4.909 4.914 3.327 4.689 4.853 4.492 4.603 4.851 4.776 4.206 5.223 4.656 4.082 4.738 NOT TAKEN 4.908 4.907 4.575 4.881 NOT TAKEN 5.014 4.611 4.929 NOT TAKEN 4.659 4.478 NOT TAKEN 5.815 4.680 4.710 4.369 4.697 4.946 2.752 4.520 4.729 4.219 4.535 4.643 4.455 4.154 4.524 3.690 4.429 4.625 NOT TAKEN 4.666 4.936 4.457 4.865 NOT TAKEN 4.932 4.641 4.927 NOT TAKEN 4.615 4.630 NOT TAKEN SPILLED 4.569 4.494 4.623 4.703 5.002 NOT TAKEN 4.582 4.688 4.185 4.393 4.700 4.501 4.042 4.817 5.119 4.513 4.832 NOT TAKEN 4.633 4.959 4.656 4.450 NOT TAKEN 4.877 4.782 4.706 NOT TAKEN 4.482 4.382 NOT TAKEN 6.394 4.410 4.587 4.436 4.438 5.025 NOT TAKEN 4.314 4.744 4.068 3.850 5.021 4.487 4.438 4.469 4.925 4.434 NOT TAKEN NOT TAKEN 4.548 4.977 4.658 5.154 NOT TAKEN 4.795 4.627 4.970 NOT TAKEN 4.465 4.437 NOT TAKEN SPILLED 4.656 4.541 4.498 4.757 5.040 NOT TAKEN 4.459 4.469 4.017 4.501 4.827 4.635 4.394 4.938 4.807 4.626 NOT TAKEN NOT TAKEN 4.585 4.615 4.464 4.885 NOT TAKEN 4.670 3.952 4.961 NOT TAKEN 4.367 4.539 NOT TAKEN 5.420 4.539 4.456 4.420 4.832 4.936 NOT TAKEN 4.543 4.624 4.335 4.362 4.708 4.490 4.351 4.771 4.965 4.570 NOT TAKEN NOT TAKEN 4.474 4.724 4.505 4.642 NOT TAKEN 3.223 5.208 4.904 NOT TAKEN 4.507 4.727 NOT TAKEN SPILLED 4.527 4.474 4.659 4.730 5.039 NOT TAKEN 4.410 4.472 4.272 4.223 4.642 4.432 4.454 2.989 4.763 4.572 NOT TAKEN NOT TAKEN 4.297 4.838 4.295 4.645 NOT TAKEN 4.758 4.983 4.911 NOT TAKEN 4.413 4.761 NOT TAKEN 5.399 4.462 4.383 4.389 4.552 4.921 NOT TAKEN 4.282 4.236 3.890 4.079 4.502 4.502 4.032 4.279 SPILLED 4.135 NOT TAKEN NOT TAKEN 4.268 4.627 4.015 4.481 NOT TAKEN 4.303 5.187 4.756 NOT TAKEN 4.427 4.295 NOT TAKEN 5.027 4.454 4.420 4.001 4.240 4.564 NOT TAKEN 4.178 4.200 3.845 4.030 4.399 4.393 4.053 4.389 4.274 4.157 NOT TAKEN NOT TAKEN 4.077 4.887 4.049 4.810 NOT TAKEN 4.506 4.912 4.809 NOT TAKEN 4.138 4.259 NOT TAKEN SPILLED 4.386 4.514 3.728 4.426 4.226 NOT TAKEN 4.283 4.220 3.974 4.309 4.659 4.364 3.788 4.656 4.312 4.095 NOT TAKEN NOT TAKEN 4.177 1412 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 30 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 4 - 100 PPM C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 4.491 4.466 4.864 NOT TAKEN 4.762 4.847 5.241 5.062 4.473 4.439 4.823 NOT TAKEN 4.505 4.752 5.055 4.731 4.490 4.295 2.777 NOT TAKEN 4.624 4.687 5.052 4.802 4.599 4.285 5.388 NOT TAKEN 4.544 4.238 4.964 4.702 4.485 4.413 SPILLED NOT TAKEN 4.542 4.692 4.700 4.786 4.386 4.449 5.405 NOT TAKEN 4.381 4.671 4.719 4.713 4.479 4.508 SPILLED NOT TAKEN 4.297 4.608 4.813 4.723 4.248 4.284 5.482 NOT TAKEN 4.143 4.387 4.636 4.296 4.309 4.054 4.612 NOT TAKEN 4.185 4.022 4.806 4.173 3.998 3.909 SPILLED NOT TAKEN 4.246 4.263 4.557 3.973 GROUP: MALE 6 - 100 PPM C91041 C91042 C91043 C91044 C91045 C91046 C91047 C91048 C91049 4.766 5.147 5.396 5.409 4.744 NOT TAKEN 5.198 5.197 5.053 4.497 5.143 5.073 5.140 4.736 NOT TAKEN 5.172 4.999 4.745 4.493 5.171 5.161 5.105 4.571 NOT TAKEN 4.855 5.154 4.745 4.340 4.757 4.926 4.958 4.464 NOT TAKEN 5.108 4.689 4.643 4.443 4.781 4.808 4.983 4.313 NOT TAKEN 4.941 4.789 4.766 4.390 4.945 4.900 4.912 4.472 NOT TAKEN 4.636 5.025 4.704 4.234 4.823 4.856 4.939 4.323 NOT TAKEN 4.745 4.837 4.700 4.127 4.512 4.889 4.960 4.222 NOT TAKEN 4.896 4.774 4.602 4.039 4.553 4.619 4.776 4.102 NOT TAKEN 4.571 4.366 4.041 3.998 4.468 4.443 4.731 3.969 NOT TAKEN 4.017 4.222 4.182 1413 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 31 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 6 - 100 PPM C91050 C91051 C91052 C91053 C91054 C91055 C91056 C91057 C91058 C91059 C91060 C91061 C91062 C91063 C91064 C91065 C91066 C91067 C91068 C91069 C91070 C91071 C91072 C91073 C91074 C91075 C91076 C91077 C91078 C91079 C91080 5.270 5.171 4.966 4.843 4.539 4.821 5.574 4.565 4.216 4.907 5.391 4.748 4.929 4.714 4.661 4.880 4.711 4.875 3.853 5.518 4.657 4.912 4.865 5.015 4.815 5.359 5.192 4.986 4.464 4.813 4.977 5.091 4.970 4.730 4.797 4.415 4.535 5.492 4.552 4.086 4.744 4.979 4.811 4.706 5.038 4.631 4.752 4.640 4.720 NOT TAKEN 5.177 4.448 4.664 4.644 4.754 4.652 5.146 5.066 4.801 4.375 4.620 4.843 4.952 4.979 SPILLED 4.840 4.542 4.519 5.345 4.369 4.291 4.671 5.172 4.892 4.771 4.958 4.607 4.978 4.691 4.726 NOT TAKEN 4.857 4.360 4.810 4.863 4.697 4.855 5.245 4.752 4.868 4.409 4.648 4.984 4.809 4.833 4.433 4.824 4.326 4.626 5.552 4.412 4.358 4.624 5.070 4.779 4.717 4.808 4.726 4.796 4.653 4.552 NOT TAKEN 4.708 4.484 4.599 4.667 4.726 4.559 4.858 4.785 4.953 4.355 4.680 4.781 4.880 4.774 4.668 4.805 4.418 4.723 5.359 4.427 4.372 4.528 5.228 4.532 4.655 4.779 4.692 4.844 4.543 4.546 NOT TAKEN 4.740 4.668 4.648 4.653 4.460 4.495 4.989 4.744 5.002 4.169 4.506 4.701 5.012 4.676 4.511 4.762 4.902 4.424 5.222 4.510 4.203 4.311 5.069 4.544 4.718 4.695 4.637 4.734 4.366 4.537 NOT TAKEN 4.671 4.507 4.408 4.614 4.540 4.587 4.831 4.523 4.867 4.484 4.638 4.633 5.101 4.810 4.519 4.645 4.668 4.424 4.783 4.205 4.126 4.409 5.103 4.449 4.482 4.767 4.395 4.388 4.429 4.350 NOT TAKEN 4.647 4.339 4.311 4.398 4.039 4.633 4.912 4.931 4.640 4.459 4.686 4.776 4.612 4.661 4.122 4.491 4.300 4.186 4.836 4.152 4.125 4.340 4.663 4.415 4.471 4.367 4.387 4.507 4.364 4.352 NOT TAKEN 4.191 4.462 4.297 4.371 4.133 4.389 4.818 4.347 4.405 4.022 4.290 4.601 4.460 4.413 3.786 4.292 3.863 4.070 SPILLED 3.852 3.938 4.270 4.427 4.020 4.463 4.434 4.201 4.326 4.109 4.272 NOT TAKEN 4.213 4.159 4.221 4.229 3.856 4.282 4.520 4.253 4.683 3.935 4.285 4.318 4.565 4.509 3.911 4.165 4.195 3.980 SPILLED 4.003 4.182 4.043 4.387 4.062 SPILLED 4.663 4.109 3.979 4.175 4.394 NOT TAKEN 4.249 4.561 4.266 4.157 3.736 4.454 SPILLED 4.257 4.837 4.198 4.163 4.375 1414 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 32 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 2 - 3 PPM C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 0.157 0.194 SPILLED 0.192 0.180 0.177 0.211 NOT TAKEN SPILLED 0.290 SPILLED 0.165 0.172 0.217 NOT TAKEN 0.185 0.192 0.181 0.159 0.163 0.183 SPILLED 0.183 0.168 0.177 0.200 NOT TAKEN 0.221 0.201 0.205 0.147 0.164 0.208 NOT TAKEN 0.163 0.180 0.184 0.158 0.147 0.183 SPILLED 0.182 0.172 0.158 SPILLED NOT TAKEN SPILLED 0.206 0.226 0.149 0.167 0.193 NOT TAKEN 0.162 0.190 0.165 0.163 0.170 NOT TAKEN 0.201 0.189 0.170 0.151 0.201 NOT TAKEN 0.290 0.188 0.196 0.150 0.174 0.168 NOT TAKEN 0.178 0.174 0.184 0.178 0.163 NOT TAKEN 0.194 0.172 0.170 0.169 SPILLED NOT TAKEN 0.330 0.187 0.176 0.164 0.176 0.204 NOT TAKEN 0.177 0.179 SPILLED 0.162 0.144 NOT TAKEN SPILLED 0.177 0.157 0.166 0.192 NOT TAKEN 0.219 SPILLED 0.171 0.157 0.171 0.194 NOT TAKEN 0.166 0.176 0.180 0.171 0.180 NOT TAKEN 0.237 0.175 0.188 0.162 0.185 NOT TAKEN SPILLED 0.189 0.204 0.137 0.158 0.182 NOT TAKEN 0.181 0.171 SPILLED 0.151 0.160 NOT TAKEN 0.208 0.177 0.180 0.167 0.193 NOT TAKEN 0.168 0.171 0.176 0.147 0.155 0.163 NOT TAKEN 0.186 0.172 0.178 0.166 0.154 NOT TAKEN SPILLED 0.169 0.175 0.153 0.172 NOT TAKEN 0.168 0.176 0.188 0.147 0.139 0.147 NOT TAKEN 0.170 0.175 0.183 0.170 0.146 NOT TAKEN 0.170 0.161 0.171 0.160 0.178 NOT TAKEN 0.179 0.164 0.167 0.139 0.143 0.166 NOT TAKEN 0.161 0.171 0.170 0.183 1415 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 33 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 2 - 3 PPM C91210 C91211 C91212 C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 SPILLED 0.157 0.188 0.198 0.183 0.183 0.248 0.193 SPILLED SPILLED 0.172 0.187 NOT TAKEN 0.186 0.236 0.176 0.184 0.179 0.184 0.168 NOT TAKEN 0.163 0.203 0.195 0.171 SPILLED 0.198 NOT TAKEN SPILLED 0.180 0.143 SPILLED 0.170 0.180 0.169 0.149 0.191 SPILLED 0.185 SPILLED SPILLED 0.161 0.205 NOT TAKEN 0.207 0.202 0.170 0.179 0.172 0.180 0.170 NOT TAKEN 0.153 0.213 0.190 0.169 SPILLED 0.192 NOT TAKEN 0.265 0.173 0.135 0.323 0.161 0.183 0.176 0.161 0.185 0.187 0.172 SPILLED SPILLED 0.151 0.192 NOT TAKEN 0.199 0.191 0.169 0.187 0.161 0.186 0.175 NOT TAKEN 0.156 0.192 0.173 0.173 SPILLED 0.183 NOT TAKEN 0.172 0.175 0.181 0.277 0.164 0.174 0.192 0.167 0.175 0.189 0.171 SPILLED SPILLED 0.154 0.161 NOT TAKEN NOT TAKEN 0.192 0.172 0.172 0.159 0.148 0.159 NOT TAKEN 0.159 0.179 NOT TAKEN 0.157 SPILLED 0.192 NOT TAKEN 0.171 0.172 0.185 SPILLED 0.161 0.164 0.186 0.179 0.189 0.200 0.178 SPILLED SPILLED 0.162 0.193 NOT TAKEN NOT TAKEN 0.186 0.157 0.179 0.165 0.175 0.158 NOT TAKEN 0.162 SPILLED NOT TAKEN 0.161 SPILLED 0.189 NOT TAKEN 0.173 0.178 0.161 SPILLED 0.163 0.168 0.174 0.173 0.200 0.177 0.167 SPILLED SPILLED 0.154 0.187 NOT TAKEN NOT TAKEN 0.192 0.170 0.178 0.152 0.182 0.161 NOT TAKEN 0.182 0.196 NOT TAKEN 0.162 SPILLED 0.196 NOT TAKEN 0.184 0.158 0.168 SPILLED 0.164 0.179 0.173 0.156 0.190 0.168 0.181 SPILLED SPILLED 0.152 0.203 NOT TAKEN NOT TAKEN 0.182 0.143 0.178 0.149 0.164 0.178 NOT TAKEN 0.146 0.201 NOT TAKEN 0.152 0.292 0.203 NOT TAKEN 0.168 0.180 0.158 SPILLED 0.156 0.171 0.177 0.179 0.196 0.180 0.166 0.202 SPILLED 0.165 0.174 NOT TAKEN NOT TAKEN 0.185 0.186 0.168 0.143 0.180 0.162 NOT TAKEN 0.135 0.182 NOT TAKEN 0.143 0.240 0.228 NOT TAKEN 0.180 0.163 0.197 0.229 0.173 0.164 0.128 0.148 0.188 0.183 0.177 0.192 0.217 0.146 0.156 NOT TAKEN NOT TAKEN 0.188 0.147 0.157 0.149 0.171 0.162 NOT TAKEN 0.142 0.170 NOT TAKEN 0.134 0.249 0.213 NOT TAKEN 0.165 0.167 0.149 0.223 0.137 0.168 0.160 0.147 0.200 0.178 0.177 SPILLED 0.177 0.157 0.178 NOT TAKEN NOT TAKEN 0.198 0.161 0.154 0.148 0.165 0.161 NOT TAKEN 0.145 0.171 NOT TAKEN 0.153 0.212 0.228 NOT TAKEN 0.184 0.167 0.154 1416 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 34 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 2 - 3 PPM C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 C91249 C91250 SPILLED 0.340 0.241 0.184 0.224 SPILLED SPILLED 0.181 0.193 0.189 0.176 SPILLED SPILLED 0.171 SPILLED SPILLED SPILLED SPILLED 0.176 0.177 GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 NOT TAKEN 1.879 1.921 2.061 2.193 1.967 SPILLED 1.865 SPILLED 1.930 2.003 1.768 1.945 1.665 1.705 1.915 1.974 1.832 NOT TAKEN 1.725 1.967 1.819 2.026 1.922 SPILLED 1.720 1.812 1.676 2.080 1.839 1.848 1.862 1.957 1.829 1.806 1.760 0.177 SPILLED 0.210 0.168 0.209 SPILLED 0.273 0.157 0.169 0.240 NOT TAKEN 1.690 1.973 1.844 1.995 1.832 2.566 1.667 1.703 1.635 1.962 1.763 1.829 1.661 1.608 1.642 1.788 1.639 NOT TAKEN SPILLED 0.264 0.172 0.206 SPILLED 0.233 NOT TAKEN 0.161 0.172 NOT TAKEN 1.888 1.780 1.775 1.810 1.754 SPILLED 1.633 1.752 1.777 1.931 1.647 1.838 1.639 1.663 1.749 1.874 1.555 NOT TAKEN SPILLED SPILLED 0.174 SPILLED SPILLED SPILLED NOT TAKEN 0.172 0.178 NOT TAKEN 1.792 1.940 1.892 1.826 1.785 2.356 1.784 1.777 1.912 1.958 1.929 1.879 1.922 1.726 1.741 1.786 1.711 NOT TAKEN SPILLED 0.217 0.175 0.200 SPILLED 0.222 NOT TAKEN 0.169 0.178 NOT TAKEN 1.792 1.829 1.812 1.895 1.781 2.107 1.595 1.787 1.754 2.096 1.773 1.869 1.702 1.650 1.685 1.875 1.622 NOT TAKEN SPILLED SPILLED SPILLED 0.179 SPILLED 0.212 NOT TAKEN 0.170 0.168 NOT TAKEN 1.845 1.935 1.691 1.767 1.876 1.506 1.775 1.798 1.781 1.996 1.971 1.878 1.847 1.573 1.765 1.785 1.559 NOT TAKEN 0.321 0.225 0.179 0.189 0.325 SPILLED NOT TAKEN 0.159 0.161 NOT TAKEN 1.594 1.731 1.618 1.760 1.702 SPILLED 1.559 1.732 1.723 2.002 1.856 1.729 1.577 1.555 2.109 1.904 1.651 NOT TAKEN SPILLED 0.224 0.180 0.155 SPILLED SPILLED NOT TAKEN 0.157 0.160 NOT TAKEN 1.718 1.767 1.750 1.850 1.620 SPILLED 1.684 1.761 1.574 1.818 1.802 1.707 1.689 1.555 1.771 SPILLED 1.505 NOT TAKEN 0.308 0.191 0.182 0.150 SPILLED 0.190 NOT TAKEN 0.150 0.161 NOT TAKEN 1.649 1.662 1.736 1.826 1.583 SPILLED 1.535 1.621 1.679 1.833 1.655 1.666 1.701 1.625 1.862 1.777 1.549 1417 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 35 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 3 - 3 0 PPM C91269 C91270 C91271 C91272 C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 2.042 2.166 2.819 NOT TAKEN 2.402 1.941 SPILLED 2.134 1.919 SPILLED 2.013 1.773 SPILLED 1.853 1.809 1.947 NOT TAKEN 2.252 NOT TAKEN 1.616 2.033 2.002 NOT TAKEN 1.797 2.641 1.730 2.147 SPILLED 2.202 1.702 1.876 2.097 1.739 2.078 NOT TAKEN 2.379 1.846 1.821 1.935 1.772 1.765 1.996 1.993 SPILLED 1.900 1.670 SPILLED NOT TAKEN 2.297 NOT TAKEN 1.680 1.780 1.926 NOT TAKEN 1.761 1.960 1.804 2.126 2.984 2.328 2.101 2.423 2.036 1.740 2.001 NOT TAKEN 2.357 1.781 1.655 1.714 1.790 1.723 1.770 1.891 2.546 1.813 1.638 2.094 NOT TAKEN 2.171 NOT TAKEN 1.620 1.675 1.775 NOT TAKEN 1.637 1.887 1.772 1.874 2.165 2.012 2.120 1.782 1.786 1.972 1.992 NOT TAKEN 2.334 1.984 1.996 1.897 1.836 1.810 1.978 1.865 NOT TAKEN 1.894 1.705 SPILLED NOT TAKEN 2.049 NOT TAKEN NOT TAKEN 1.822 2.125 NOT TAKEN 1.695 NOT TAKEN 1.738 2.114 2.096 SPILLED SPILLED NOT TAKEN 1.860 1.908 1.961 NOT TAKEN SPILLED SPILLED 1.777 1.999 1.733 1.770 1.918 1.947 NOT TAKEN 1.784 1.554 1.967 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.782 2.213 NOT TAKEN 1.794 NOT TAKEN 1.774 SPILLED SPILLED 2.521 SPILLED NOT TAKEN SPILLED 1.934 1.872 NOT TAKEN SPILLED 1.920 1.725 1.859 1.717 1.717 1.919 1.803 NOT TAKEN 1.795 1.667 1.903 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.730 2.204 NOT TAKEN 1.733 NOT TAKEN 1.697 1.988 SPILLED 2.061 SPILLED NOT TAKEN 1.776 1.849 1.883 NOT TAKEN 2.103 1.737 1.672 1.892 1.620 1.835 2.168 1.896 NOT TAKEN 1.706 1.681 2.108 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.817 2.214 NOT TAKEN 1.704 NOT TAKEN 1.700 1.899 2.038 2.196 SPILLED NOT TAKEN 1.556 1.734 1.899 NOT TAKEN 1.926 1.771 1.774 1.784 1.707 1.803 1.663 1.848 NOT TAKEN 1.580 1.647 1.931 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.772 2.273 NOT TAKEN 1.470 NOT TAKEN 1.611 1.655 1.872 2.219 SPILLED NOT TAKEN 1.760 1.651 1.659 NOT TAKEN 1.795 1.606 1.546 1.899 1.737 1.737 1.603 1.746 NOT TAKEN 1.676 1.619 1.831 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.822 1.583 NOT TAKEN 1.544 NOT TAKEN 1.681 1.873 1.886 SPILLED 1.639 NOT TAKEN 1.606 1.767 1.770 NOT TAKEN 1.813 1.617 1.544 1.938 1.770 1.751 1.653 1.674 NOT TAKEN 1.545 1.497 1.581 NOT TAKEN 2.167 NOT TAKEN NOT TAKEN 1.739 2.387 NOT TAKEN 1.357 NOT TAKEN 1.869 1.642 1.874 2.425 SPILLED NOT TAKEN 1418 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 36 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 3 - 3 0 PPM C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 3.423 1.799 2.701 1.655 1.914 2.030 1.838 1.726 2.657 1.658 1.937 2.049 1.857 SPILLED 1.804 1.979 2.030 2.015 1.708 2.496 1.736 2.047 GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 6.200 6.233 7.943 8.759 7.427 NOT TAKEN NOT TAKEN 5.470 6.932 6.368 6.751 6.713 6.524 6.452 5.572 7.058 6.324 6.839 6.158 6.270 SPILLED 6.698 NOT TAKEN NOT TAKEN 5.679 6.466 6.419 6.872 6.713 6.619 8.698 5.445 6.746 5.680 2.132 1.821 SPILLED 1.521 1.893 1.958 1.947 1.573 3.042 1.770 1.962 7.926 6.153 6.367 SPILLED 6.657 NOT TAKEN NOT TAKEN 5.226 6.379 6.118 6.092 6.635 6.602 5.703 5.291 6.057 6.081 2.061 1.754 1.785 1.679 NOT TAKEN 1.803 1.840 1.743 SPILLED 1.758 1.888 6.696 6.157 6.392 SPILLED 6.448 NOT TAKEN NOT TAKEN 5.495 6.313 6.572 6.836 6.419 6.851 6.127 5.641 6.083 5.974 SPILLED 1.811 1.688 1.729 NOT TAKEN 2.096 1.986 1.609 SPILLED 1.848 1.912 6.652 6.237 6.557 SPILLED 7.143 NOT TAKEN NOT TAKEN NOT TAKEN 6.540 6.398 6.775 6.661 6.726 6.045 5.527 7.522 6.090 SPILLED 1.732 1.743 1.568 NOT TAKEN 2.059 1.823 1.618 2.899 1.770 1.913 7.034 6.101 6.197 SPILLED 6.699 NOT TAKEN NOT TAKEN NOT TAKEN 6.343 5.952 6.204 6.991 6.641 6.062 5.673 6.536 6.138 SPILLED 1.777 1.721 1.686 NOT TAKEN 2.008 1.855 1.732 2.450 1.749 1.828 7.439 6.027 6.501 SPILLED 6.610 NOT TAKEN NOT TAKEN NOT TAKEN 6.452 6.507 6.759 6.439 7.180 5.922 5.560 7.151 6.320 2.113 1.780 1.842 1.483 NOT TAKEN 1.903 1.807 1.732 2.311 1.691 1.821 7.002 5.882 6.561 SPILLED 6.433 NOT TAKEN NOT TAKEN NOT TAKEN 6.230 SPILLED 6.928 NOT TAKEN 6.773 6.112 5.518 7.212 5.884 1.818 1.484 1.755 1.365 NOT TAKEN 1.861 1.768 1.559 SPILLED 1.618 1.637 7.264 6.096 6.574 SPILLED 6.413 NOT TAKEN NOT TAKEN NOT TAKEN 6.408 6.036 6.776 NOT TAKEN 7.051 5.709 5.391 7.050 5.707 1.758 1.460 1.810 1.618 NOT TAKEN 1.875 2.017 1.669 2.042 1.679 1.618 7.050 6.173 6.919 SPILLED 6.535 NOT TAKEN NOT TAKEN NOT TAKEN 6.239 5.852 6.938 NOT TAKEN 6.976 5.833 5.693 6.948 5.860 1419 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 37 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 4 - 100 PPM C91328 C91329 C91330 C91331 C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 5.945 5.906 6.135 6.538 7.249 NOT TAKEN 5.811 6.164 SPILLED 5.714 6.156 5.835 5.544 5.766 6.067 6.726 5.757 NOT TAKEN 6.267 5.736 5.822 6.032 NOT TAKEN 6.052 5.714 6.349 5.690 8.006 6.744 6.076 6.551 6.150 5.626 5.931 5.766 7.309 NOT TAKEN 6.032 5.874 SPILLED 5.871 5.701 6.161 5.351 5.530 6.084 6.524 5.532 NOT TAKEN 6.445 6.095 5.870 6.015 NOT TAKEN 5.677 5.284 6.517 5.691 7.027 6.137 5.357 5.775 5.229 5.592 5.838 5.929 6.589 NOT TAKEN 5.673 5.919 9.446 5.481 6.061 5.563 5.042 5.338 5.511 6.436 5.442 NOT TAKEN 6.149 5.640 5.726 6.134 NOT TAKEN 5.585 5.578 6.272 5.386 5.876 5.881 5.361 6.036 5.843 NOT TAKEN 5.904 6.148 5.547 NOT TAKEN 5.901 6.187 SPILLED NOT TAKEN 6.019 5.811 5.169 5.592 5.747 6.423 6.138 NOT TAKEN 6.417 5.788 5.353 6.397 NOT TAKEN 5.933 5.590 6.672 5.726 NOT TAKEN 5.872 5.263 5.874 5.970 NOT TAKEN 6.349 6.305 SPILLED NOT TAKEN 5.632 5.781 SPILLED NOT TAKEN 6.342 6.088 5.112 5.664 5.671 6.556 5.832 NOT TAKEN 6.318 5.926 6.110 6.501 NOT TAKEN 5.542 6.055 6.243 5.536 NOT TAKEN 6.448 5.463 6.239 5.492 NOT TAKEN 5.885 6.136 6.362 NOT TAKEN 5.541 6.024 SPILLED NOT TAKEN 6.349 5.809 5.438 5.454 6.040 5.909 5.799 NOT TAKEN 6.674 6.216 6.148 6.284 NOT TAKEN 5.982 5.937 6.299 5.996 NOT TAKEN 6.193 5.478 6.344 SPILLED NOT TAKEN 6.229 6.015 6.157 NOT TAKEN 5.544 6.066 SPILLED NOT TAKEN 6.009 6.106 5.272 5.830 5.840 6.689 5.508 NOT TAKEN 6.286 5.534 5.874 6.269 NOT TAKEN 5.497 6.149 6.505 SPILLED NOT TAKEN 7.026 5.741 6.139 6.510 NOT TAKEN 6.018 5.855 5.951 NOT TAKEN 5.774 5.685 SPILLED NOT TAKEN 5.726 5.923 5.979 6.287 5.942 6.555 5.740 NOT TAKEN 5.967 5.932 5.778 6.493 NOT TAKEN 5.652 5.690 6.729 5.769 NOT TAKEN 6.813 5.082 5.926 5.629 NOT TAKEN 5.644 6.061 5.665 NOT TAKEN 5.542 5.479 SPILLED NOT TAKEN 5.332 6.204 5.081 5.982 5.340 6.525 5.640 NOT TAKEN 6.139 5.676 5.905 7.411 NOT TAKEN 5.175 5.674 6.453 5.794 NOT TAKEN 6.439 5.412 5.500 5.281 NOT TAKEN 6.067 5.581 7.561 NOT TAKEN 5.577 5.456 10.100 NOT TAKEN 5.568 6.258 5.486 5.891 5.654 6.277 5.768 NOT TAKEN 5.793 5.814 6.089 7.023 NOT TAKEN 5.839 5.960 6.566 5.907 NOT TAKEN SPILLED 5.373 6.236 1420 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 38 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 4 - 100 PPM C91359 C91360 C91361 C91362 C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 SPILLED 6.356 4.595 7.673 6.015 5.918 15.524 6.092 6.023 5.886 6.314 6.730 SPILLED 6.195 5.833 5.662 6.122 5.907 7.072 6.624 6.509 6.161 8.246 6.140 5.347 8.148 6.186 5.808 SPILLED 5.370 5.549 6.090 5.821 6.701 7.742 6.291 5.522 5.942 6.091 5.703 6.945 5.691 6.971 6.273 10.138 6.122 5.408 6.253 5.964 5.528 14.975 5.497 5.510 5.737 6.525 6.659 7.046 5.892 6.356 5.459 6.228 5.501 6.343 5.657 6.646 5.375 7.745 6.144 5.236 SPILLED 6.238 5.864 14.839 6.191 6.074 5.378 6.126 NOT TAKEN 5.762 5.577 6.291 4.838 5.993 5.714 6.209 6.343 NOT TAKEN 6.040 7.664 6.283 5.367 5.850 5.970 5.361 SPILLED 5.563 5.666 5.692 6.090 NOT TAKEN 5.950 5.944 6.204 6.010 5.874 5.825 7.033 6.255 NOT TAKEN 6.000 6.146 6.077 5.107 5.693 5.498 5.622 SPILLED 5.694 5.467 5.560 6.327 NOT TAKEN 5.259 5.846 6.392 5.684 5.843 5.692 6.790 5.837 NOT TAKEN 5.657 6.417 6.478 5.384 6.866 6.280 5.838 SPILLED 5.917 6.000 5.796 SPILLED NOT TAKEN 6.143 5.870 6.068 5.739 5.823 5.698 7.023 6.119 NOT TAKEN 6.029 6.485 6.546 5.429 6.458 5.732 5.618 13.289 5.793 6.181 5.570 6.132 NOT TAKEN SPILLED 5.470 6.398 5.702 6.012 5.706 6.897 6.015 NOT TAKEN 5.807 6.943 6.259 5.081 5.673 5.183 5.725 SPILLED 5.760 5.697 5.459 5.942 NOT TAKEN 6.127 4.969 6.359 5.148 5.757 5.532 6.245 5.808 NOT TAKEN 5.547 6.817 6.681 4.845 5.732 6.220 6.026 SPILLED 6.049 5.781 5.569 6.155 NOT TAKEN 5.820 8.230 5.984 5.531 5.953 5.780 6.173 5.590 NOT TAKEN 5.858 1421 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 39 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 6 - 100 PPM C91451 C91452 C91453 C91454 C91455 C91456 C91457 C91458 C91459 C91460 C91461 C91462 C91463 C91464 C91465 C91466 C91467 C91468 C91469 C91470 C91471 C91472 C91473 C91474 C91475 C91476 6.616 6.148 6.228 6.502 6.723 6.012 6.295 6.186 SPILLED 10.941 6.557 6.893 5.165 6.555 7.003 6.074 6.199 6.188 5.974 6.376 6.184 SPILLED 6.621 SPILLED 7.169 5.939 6.324 6.141 6.498 5.960 6.317 5.901 6.651 5.844 SPILLED 6.521 5.602 6.447 5.381 6.184 7.112 5.102 5.948 6.862 5.793 6.604 6.114 SPILLED 10.336 7.961 6.097 5.939 6.293 5.884 6.153 5.784 5.933 5.795 6.698 5.967 SPILLED 5.941 5.223 6.213 4.934 6.335 6.691 5.472 5.514 6.342 5.625 6.543 6.018 6.520 9.406 5.685 5.963 5.948 6.086 5.685 6.970 6.624 5.864 5.933 6.218 5.556 SPILLED 6.379 5.785 6.643 5.442 6.070 6.593 5.342 6.103 6.140 5.989 6.185 6.178 5.850 SPILLED 5.895 5.973 6.018 6.086 5.774 6.569 6.400 6.036 5.884 6.327 6.349 SPILLED 6.211 5.793 6.252 5.768 6.256 6.653 5.333 5.834 5.790 5.551 6.460 6.349 6.951 6.164 6.176 6.388 5.621 6.325 5.989 6.372 5.838 6.337 5.648 6.416 5.938 SPILLED 5.898 6.323 6.129 5.589 5.895 6.880 5.375 5.574 SPILLED 5.391 6.077 6.169 6.231 5.393 5.948 6.150 5.890 6.467 5.911 6.060 6.105 6.399 5.872 6.474 6.355 SPILLED 6.686 6.313 6.286 5.626 7.377 6.081 5.666 6.062 SPILLED 5.744 6.111 6.230 6.126 5.841 6.664 6.336 6.297 6.312 5.874 6.005 5.757 6.082 5.879 5.925 6.004 SPILLED 5.876 6.266 6.180 5.541 6.142 7.113 5.262 6.040 SPILLED 5.764 6.189 6.358 5.753 6.489 5.939 6.122 6.052 6.304 5.721 6.310 6.042 5.811 5.561 5.649 6.034 SPILLED 5.993 5.850 5.716 5.772 6.328 6.901 4.954 6.189 5.805 5.589 5.774 6.128 5.451 5.468 6.911 5.736 6.245 6.299 6.221 5.889 6.180 5.892 5.724 5.864 6.241 SPILLED 6.098 5.483 5.896 5.776 6.253 6.935 5.182 6.542 8.047 6.094 6.214 6.119 5.518 SPILLED 5.694 5.938 5.830 1422 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 6 - 100 PPM C91477 C91478 C91479 C91480 C91481 C91482 C91483 C91484 C91485 C91486 C91487 C91488 C91489 C91490 6.973 7.038 6.884 6.255 5.641 6.576 5.801 6.175 6.073 5.801 6.255 6.172 7.914 6.686 6.343 7.221 6.822 5.825 5.603 6.226 5.725 5.915 5.862 5.832 5.837 5.971 6.699 6.331 6.107 6.252 6.475 5.659 5.727 6.044 5.185 5.845 5.508 5.432 5.336 6.344 6.670 6.300 6.672 6.389 6.699 6.024 5.888 6.627 5.693 5.973 4.879 5.827 5.492 5.900 6.484 6.146 6.438 6.926 6.528 5.825 6.161 6.325 5.725 6.057 5.714 5.664 5.559 6.146 6.526 6.423 6.419 7.357 6.893 5.725 5.655 5.895 6.211 5.682 5.399 5.664 5.416 5.505 6.941 6.154 6.241 6.700 6.892 5.810 5.840 6.072 6.600 5.185 5.918 5.902 6.191 5.763 7.249 6.387 6.540 6.682 6.367 5.745 5.762 5.984 5.685 5.660 5.777 5.913 6.203 5.542 6.305 6.061 Covance 6329-212 3M T-6316.1 PAGE: 40 WEEK 28 WEEK 32 6.421 6.561 6.279 6.112 5.622 5.731 5.888 5.885 5.948 6.410 5.892 5.442 6.725 5.944 6.371 SPILLED SPILLED 6.409 5.913 6.362 6.161 5.964 5.940 6.193 6.103 5.381 6.099 6.049 1423 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 41 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 0.116 0.118 0.119 0.130 0.125 0.123 0.126 0.111 NOT TAKEN 0.124 0.137 0.130 0.116 SPILLED 0.116 0.129 NOT TAKEN 0.123 0.124 0.115 0.127 0.134 0.115 0.118 0.124 NOT TAKEN NOT TAKEN 0.118 0.129 0.114 0.122 0.120 0.128 0.133 0.141 0.137 0.131 0.129 0.118 NOT TAKEN 0.131 0.148 0.133 0.122 SPILLED 0.115 0.138 NOT TAKEN 0.129 0.129 0.123 0.136 0.135 0.124 0.120 0.122 NOT TAKEN NOT TAKEN 0.129 0.139 0.124 0.126 0.109 0.114 0.122 0.126 0.119 0.119 0.115 0.103 NOT TAKEN 0.120 0.129 0.121 0.116 0.129 0.107 0.128 NOT TAKEN 0.111 0.119 0.114 0.121 0.121 0.110 0.110 0.113 NOT TAKEN NOT TAKEN 0.116 0.120 0.110 0.111 0.108 0.110 0.113 0.119 0.122 0.113 0.115 0.101 NOT TAKEN 0.112 0.125 0.119 0.112 0.115 0.102 0.123 NOT TAKEN 0.111 0.114 0.109 0.104 0.123 0.106 0.107 0.106 NOT TAKEN NOT TAKEN 0.104 0.123 0.105 0.150 0.102 0.107 0.108 0.116 0.109 0.102 0.108 0.096 NOT TAKEN 0.105 0.120 0.110 0.106 0.101 0.063 0.121 NOT TAKEN 0.104 0.111 0.107 0.134 0.115 0.103 0.100 0.117 NOT TAKEN NOT TAKEN 0.104 0.127 0.097 0.126 0.103 0.107 0.111 0.114 0.108 0.101 0.102 0.091 NOT TAKEN 0.109 0.120 0.008 0.103 0.098 0.016 0.108 NOT TAKEN 0.106 0.106 0.107 0.118 0.109 0.099 0.098 0.033 NOT TAKEN NOT TAKEN 0.100 0.121 0.091 0.107 0.098 0.107 0.108 0.115 0.107 0.107 0.106 0.096 NOT TAKEN 0.102 0.120 NOT TAKEN 0.106 0.100 NOT TAKEN NOT TAKEN NOT TAKEN 0.106 0.108 0.105 0.118 0.110 0.104 0.099 0.015 NOT TAKEN NOT TAKEN 0.107 0.119 0.099 0.088 0.089 0.105 0.106 0.109 0.103 0.114 0.100 0.089 NOT TAKEN 0.106 0.108 NOT TAKEN 0.102 0.091 NOT TAKEN NOT TAKEN NOT TAKEN 0.100 0.106 0.106 0.111 0.103 SPILLED 0.101 NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.114 0.096 0.100 0.102 0.099 0.102 0.112 0.104 0.104 0.101 0.091 NOT TAKEN 0.096 0.115 NOT TAKEN 0.102 0.092 NOT TAKEN NOT TAKEN NOT TAKEN 0.111 0.106 0.108 0.106 0.112 0.119 0.099 NOT TAKEN NOT TAKEN NOT TAKEN 0.025 0.115 0.097 0.102 0.099 0.110 0.110 0.115 0.108 0.107 0.100 0.090 NOT TAKEN 0.108 0.115 NOT TAKEN 0.100 0.098 NOT TAKEN NOT TAKEN NOT TAKEN 0.107 0.108 0.110 0.108 0.115 0.139 0.098 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.117 0.098 0.103 1424 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 42 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 2 - 3 PPM C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 0.119 NOT TAKEN NOT TAKEN 0.120 0.116 NOT TAKEN NOT TAKEN 0.111 0.112 0.119 0.131 0.116 0.119 0.114 0.155 0.118 0.114 0.118 0.115 NOT TAKEN 0.116 NOT TAKEN NOT TAKEN 0.117 NOT TAKEN 0.118 0.111 0.112 0.106 0.131 NOT TAKEN NOT TAKEN 0.137 0.120 NOT TAKEN NOT TAKEN 0.122 0.117 0.123 0.134 0.125 0.132 0.124 SPILLED 0.124 0.117 0.118 0.126 NOT TAKEN 0.126 NOT TAKEN NOT TAKEN 0.121 NOT TAKEN 0.124 0.095 0.121 0.118 0.118 NOT TAKEN NOT TAKEN 0.121 0.103 NOT TAKEN NOT TAKEN 0.111 0.107 0.112 0.120 0.113 0.115 0.114 0.130 0.114 0.110 0.107 0.112 NOT TAKEN 0.105 NOT TAKEN NOT TAKEN 0.119 NOT TAKEN 0.111 0.106 0.104 0.103 0.112 NOT TAKEN NOT TAKEN 0.113 0.104 NOT TAKEN NOT TAKEN 0.102 0.102 0.112 0.114 0.112 0.108 0.108 0.129 0.114 0.114 0.099 0.107 NOT TAKEN 0.109 NOT TAKEN NOT TAKEN 0.118 NOT TAKEN 0.110 0.098 0.101 0.093 0.107 NOT TAKEN NOT TAKEN 0.117 0.111 NOT TAKEN NOT TAKEN 0.101 0.095 0.098 0.112 0.099 0.102 0.114 0.127 0.112 0.122 0.096 0.104 NOT TAKEN 0.106 NOT TAKEN NOT TAKEN 0.118 NOT TAKEN 0.131 0.096 0.096 0.097 0.102 NOT TAKEN NOT TAKEN 0.100 0.103 NOT TAKEN NOT TAKEN 0.093 0.095 0.096 0.106 0.102 0.098 0.103 0.119 0.110 0.095 0.090 0.099 NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 0.103 NOT TAKEN 0.183 0.081 0.091 0.090 0.107 NOT TAKEN NOT TAKEN 0.107 0.107 NOT TAKEN NOT TAKEN 0.094 0.095 0.098 0.105 0.097 0.101 0.099 0.117 0.100 0.107 0.091 0.101 NOT TAKEN 0.096 NOT TAKEN NOT TAKEN 0.111 NOT TAKEN 0.231 0.089 0.090 0.091 0.107 NOT TAKEN NOT TAKEN 0.100 0.095 NOT TAKEN NOT TAKEN 0.097 0.099 0.099 0.103 0.098 0.096 0.097 0.068 0.103 0.114 0.089 0.103 NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 0.112 NOT TAKEN 0.201 0.089 0.094 0.092 0.114 NOT TAKEN NOT TAKEN 0.104 0.096 NOT TAKEN NOT TAKEN 0.094 0.044 0.099 0.103 0.094 0.095 0.103 0.111 NOT TAKEN 0.120 0.091 0.101 NOT TAKEN 0.098 NOT TAKEN NOT TAKEN 0.117 NOT TAKEN SPILLED 0.089 0.096 0.092 0.109 NOT TAKEN NOT TAKEN 0.103 0.100 NOT TAKEN NOT TAKEN 0.098 NOT TAKEN 0.095 0.092 0.107 0.099 0.098 0.112 NOT TAKEN 0.116 0.095 0.105 NOT TAKEN 0.033 NOT TAKEN NOT TAKEN 0.107 NOT TAKEN 0.297 0.094 0.088 0.088 1425 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 43 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 3 - 3 0 PPM C90841 C90842 C90843 C90844 C90845 C90846 C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 1.279 1.256 NOT TAKEN 1.256 1.131 NOT TAKEN 1.305 1.107 1.137 1.135 1.205 NOT TAKEN NOT TAKEN 1.338 1.257 1.158 1.178 1.319 1.395 1.557 1.314 1.336 NOT TAKEN 1.306 1.313 NOT TAKEN 1.320 1.140 1.232 1.359 1.167 1.227 1.283 NOT TAKEN 1.405 1.231 NOT TAKEN 1.355 1.217 1.150 1.269 1.293 NOT TAKEN NOT TAKEN 1.558 1.279 1.249 1.297 1.369 1.492 1.527 1.279 1.400 NOT TAKEN 1.341 1.343 NOT TAKEN 1.488 1.207 1.296 1.384 1.283 1.173 1.208 NOT TAKEN 1.212 1.055 NOT TAKEN 1.239 1.149 1.101 1.102 1.151 NOT TAKEN NOT TAKEN 1.258 1.107 1.111 1.155 1.289 1.342 1.373 1.227 1.263 NOT TAKEN 1.214 1.212 NOT TAKEN 1.343 1.089 1.161 1.135 1.167 1.109 1.150 NOT TAKEN 1.138 1.059 NOT TAKEN 1.146 1.023 1.046 1.041 1.135 NOT TAKEN NOT TAKEN 1.166 1.130 1.070 1.244 1.207 1.283 1.380 1.157 1.168 NOT TAKEN 1.179 1.231 NOT TAKEN 1.259 1.006 1.098 1.126 1.097 1.153 1.136 NOT TAKEN 1.156 1.058 NOT TAKEN 1.110 1.026 1.032 1.096 1.120 NOT TAKEN NOT TAKEN 1.115 1.075 0.988 1.129 1.150 1.232 1.323 1.161 1.133 NOT TAKEN 1.141 1.192 NOT TAKEN 1.289 1.039 1.102 1.092 1.128 1.018 1.041 NOT TAKEN 0.996 0.997 NOT TAKEN 1.021 0.922 0.977 1.049 1.041 NOT TAKEN NOT TAKEN 1.089 0.988 0.927 1.087 1.104 1.034 1.306 1.086 1.108 NOT TAKEN 1.100 1.100 NOT TAKEN 1.303 1.004 1.048 1.082 1.059 1.052 1.084 NOT TAKEN 1.153 0.989 NOT TAKEN 1.078 0.937 0.916 1.048 1.100 NOT TAKEN NOT TAKEN 1.138 1.011 1.061 1.054 1.071 0.984 1.296 1.142 1.092 NOT TAKEN 1.075 1.155 NOT TAKEN 1.310 0.965 1.046 1.063 1.116 1.049 0.980 NOT TAKEN 1.059 1.005 NOT TAKEN 1.046 0.823 0.813 0.961 0.994 NOT TAKEN NOT TAKEN 1.078 0.920 0.987 1.089 1.024 0.597 1.181 0.794 0.567 NOT TAKEN 1.124 1.077 NOT TAKEN 1.293 0.967 1.022 1.052 1.018 1.039 1.039 NOT TAKEN 1.072 0.985 NOT TAKEN 1.097 0.866 0.729 0.977 1.010 NOT TAKEN NOT TAKEN 1.156 0.955 0.948 1.099 1.050 1.455 1.196 0.547 0.881 NOT TAKEN 1.094 1.133 NOT TAKEN 1.250 0.974 0.813 1.011 1.043 1.116 1.086 NOT TAKEN 1.120 1.019 NOT TAKEN 1.121 0.773 0.678 1.071 1.074 NOT TAKEN NOT TAKEN 1.105 1.001 0.985 1.131 1.043 NOT TAKEN 1.287 NOT TAKEN NOT TAKEN NOT TAKEN 1.052 1.091 NOT TAKEN 1.408 0.367 1.098 0.878 1.035 1426 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 44 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 3 - 3 0 PPM C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 1.183 1.246 1.107 1.171 SPILLED NOT TAKEN 1.368 1.282 NOT TAKEN 1.164 1.212 1.461 1.517 1.206 1.240 1.137 1.341 1.179 NOT TAKEN NOT TAKEN 1.290 1.199 1.242 1.260 1.399 1.187 1.227 1.357 1.300 1.311 1.288 1.169 1.251 1.265 NOT TAKEN 1.448 1.253 NOT TAKEN 1.248 1.257 1.660 1.549 1.273 1.316 1.287 1.525 1.273 NOT TAKEN NOT TAKEN 1.381 1.391 1.289 1.384 1.477 1.254 1.334 1.464 1.411 1.157 1.157 1.066 1.174 1.108 NOT TAKEN 1.268 1.184 NOT TAKEN 1.132 1.134 1.522 1.429 1.162 1.178 1.145 1.300 1.168 NOT TAKEN NOT TAKEN 1.237 1.209 1.269 1.191 1.332 1.131 1.113 1.282 1.255 1.085 1.079 1.011 1.136 1.025 NOT TAKEN 1.252 1.187 NOT TAKEN 1.078 1.123 1.511 1.250 1.088 1.169 1.085 1.132 1.116 NOT TAKEN NOT TAKEN 1.154 1.220 1.202 1.194 1.281 1.070 1.167 1.260 1.215 1.051 SPILLED 1.000 1.071 1.040 NOT TAKEN 1.271 1.051 NOT TAKEN 1.032 1.095 1.021 1.339 1.053 1.049 1.061 1.168 1.134 NOT TAKEN NOT TAKEN 1.140 1.181 1.136 1.137 1.285 1.068 1.139 1.272 1.203 1.017 1.122 0.178 1.040 NOT TAKEN NOT TAKEN NOT TAKEN 1.059 NOT TAKEN 0.975 1.013 1.480 1.363 0.975 1.122 1.019 1.129 1.003 NOT TAKEN NOT TAKEN 1.058 1.086 1.039 1.078 1.301 1.023 1.220 1.238 1.158 1.048 1.069 1.231 1.114 NOT TAKEN NOT TAKEN NOT TAKEN 1.106 NOT TAKEN 1.018 0.999 1.418 1.397 0.994 1.066 0.924 1.205 1.109 NOT TAKEN NOT TAKEN 1.082 1.030 1.046 1.049 1.088 0.999 0.223 1.173 1.104 1.033 SPILLED 1.363 1.039 NOT TAKEN NOT TAKEN NOT TAKEN 1.104 NOT TAKEN 0.974 0.962 1.169 SPILLED 1.039 0.908 0.896 1.369 1.018 NOT TAKEN NOT TAKEN 1.145 0.975 0.977 1.011 0.813 0.956 NOT TAKEN 1.077 1.034 1.024 0.986 NOT TAKEN 1.044 NOT TAKEN NOT TAKEN NOT TAKEN 1.190 NOT TAKEN 1.115 0.953 1.257 SPILLED 0.985 1.050 0.000 1.256 1.038 NOT TAKEN NOT TAKEN 1.018 1.032 0.940 0.980 0.465 0.986 NOT TAKEN 1.073 1.030 1.061 0.916 NOT TAKEN 1.095 NOT TAKEN NOT TAKEN NOT TAKEN 1.118 NOT TAKEN 1.069 1.016 1.198 SPILLED 0.946 1.076 NOT TAKEN 1.402 1.041 NOT TAKEN NOT TAKEN 0.648 0.999 1.057 1.079 NOT TAKEN 0.992 NOT TAKEN 1.070 1.066 1427 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 45 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 4.325 4.144 4.137 4.457 NOT TAKEN 4.475 NOT TAKEN 3.799 4.105 4.119 4.276 4.291 3.980 3.895 4.242 4.384 NOT TAKEN 4.434 4.333 3.979 NOT TAKEN 4.408 4.765 4.081 4.629 4.289 4.520 4.204 4.617 4.649 4.188 4.592 4.363 4.403 4.738 NOT TAKEN 4.654 NOT TAKEN 4.167 4.116 4.289 4.477 4.538 4.359 3.982 4.214 4.586 NOT TAKEN 4.399 4.368 4.282 NOT TAKEN 4.471 4.460 4.361 4.805 4.410 4.714 4.512 4.596 4.834 4.526 4.202 3.921 4.101 4.361 NOT TAKEN 4.206 NOT TAKEN 3.579 4.005 3.857 4.204 4.118 3.860 3.722 4.026 4.147 NOT TAKEN 4.094 4.277 3.992 NOT TAKEN 4.103 4.553 4.383 4.615 4.191 3.912 3.925 3.755 4.404 4.148 4.065 3.872 3.951 4.168 NOT TAKEN 3.893 NOT TAKEN 3.676 3.655 3.558 3.943 4.038 3.887 3.534 3.889 3.979 NOT TAKEN 3.941 4.095 3.995 NOT TAKEN 3.894 4.201 4.243 4.183 3.999 3.989 3.861 3.768 4.117 3.934 4.003 3.773 3.557 4.124 NOT TAKEN 3.808 NOT TAKEN 3.758 3.447 3.479 3.933 3.923 3.558 3.653 3.908 3.825 NOT TAKEN 3.615 4.093 4.005 NOT TAKEN 3.961 4.221 4.156 4.170 4.104 3.945 3.757 3.615 4.155 3.527 3.903 NOT TAKEN 3.513 3.915 NOT TAKEN 3.711 NOT TAKEN 3.859 NOT TAKEN 3.304 3.714 3.865 NOT TAKEN 3.574 3.731 3.607 NOT TAKEN NOT TAKEN 3.794 3.952 NOT TAKEN 3.697 4.247 NOT TAKEN 4.061 3.468 3.697 3.962 4.249 4.039 3.570 3.659 NOT TAKEN 3.523 3.937 NOT TAKEN 3.481 NOT TAKEN 3.632 NOT TAKEN 3.269 3.528 3.786 NOT TAKEN 3.369 3.589 3.439 NOT TAKEN NOT TAKEN 3.787 3.923 NOT TAKEN 2.906 4.063 NOT TAKEN 3.856 3.384 3.622 3.720 3.425 3.881 3.536 3.771 NOT TAKEN 3.375 3.913 NOT TAKEN 3.282 NOT TAKEN 3.839 NOT TAKEN 3.405 3.468 2.336 NOT TAKEN 3.466 3.585 3.491 NOT TAKEN NOT TAKEN 3.833 3.603 NOT TAKEN NOT TAKEN 3.940 NOT TAKEN 3.649 3.194 3.369 3.548 3.804 3.637 3.410 3.755 NOT TAKEN 3.600 3.850 NOT TAKEN 3.574 NOT TAKEN 3.806 NOT TAKEN 3.389 3.582 3.207 NOT TAKEN 3.634 3.698 3.565 NOT TAKEN NOT TAKEN 4.133 3.913 NOT TAKEN NOT TAKEN 3.989 NOT TAKEN 3.875 3.314 3.624 3.479 NOT TAKEN 4.005 3.190 2.387 NOT TAKEN 3.464 3.764 NOT TAKEN 3.721 NOT TAKEN 3.774 NOT TAKEN 3.431 3.535 3.136 NOT TAKEN 3.497 3.669 3.609 NOT TAKEN NOT TAKEN 4.192 4.236 NOT TAKEN NOT TAKEN 4.172 NOT TAKEN 3.874 3.515 3.714 3.724 NOT TAKEN 4.367 3.525 1428 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 46 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 4 - 100 PPM C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 5.156 4.279 4.858 NOT TAKEN 4.376 5.060 4.751 NOT TAKEN 4.245 4.285 NOT TAKEN 5.510 4.267 4.435 3.180 4.179 4.337 NOT TAKEN 4.221 4.214 4.014 3.957 4.582 4.376 4.130 4.526 4.459 4.375 NOT TAKEN NOT TAKEN 4.289 5.310 4.330 4.840 NOT TAKEN 3.991 5.997 4.767 NOT TAKEN 4.735 4.583 NOT TAKEN 5.048 4.528 4.443 4.498 4.680 4.670 NOT TAKEN 4.592 4.436 4.305 4.184 4.797 4.561 4.626 4.289 3.483 4.580 NOT TAKEN NOT TAKEN 4.474 4.780 3.753 4.620 NOT TAKEN 4.584 4.847 4.483 NOT TAKEN 4.176 3.794 NOT TAKEN SPILLED 4.254 NOT TAKEN 2.861 4.034 4.071 NOT TAKEN 3.969 3.837 3.625 3.909 4.353 4.141 3.948 4.293 4.049 4.143 NOT TAKEN NOT TAKEN 4.020 4.406 3.867 4.350 NOT TAKEN 4.285 4.715 4.160 NOT TAKEN 4.346 3.936 NOT TAKEN 4.391 4.105 NOT TAKEN 3.554 3.920 3.940 NOT TAKEN 4.011 3.865 3.352 3.725 4.129 3.990 4.001 4.519 4.082 3.952 NOT TAKEN NOT TAKEN 3.887 4.236 3.088 4.817 NOT TAKEN 3.531 4.259 4.147 NOT TAKEN 3.893 3.804 NOT TAKEN 4.209 3.758 NOT TAKEN 3.928 3.900 4.007 NOT TAKEN 3.584 3.842 2.767 3.736 3.915 3.850 3.854 4.182 3.614 3.764 NOT TAKEN NOT TAKEN 3.590 NOT TAKEN 3.902 4.504 NOT TAKEN 3.948 4.233 3.802 NOT TAKEN 3.871 3.580 NOT TAKEN 4.608 3.817 NOT TAKEN 4.117 3.579 3.798 NOT TAKEN NOT TAKEN NOT TAKEN 1.492 3.592 3.681 3.683 3.911 NOT TAKEN 3.929 3.587 NOT TAKEN NOT TAKEN 3.612 NOT TAKEN 3.430 4.519 NOT TAKEN 3.928 4.185 4.008 NOT TAKEN 2.244 3.549 NOT TAKEN 4.657 3.927 NOT TAKEN 3.358 3.584 3.673 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.528 3.741 3.654 3.803 NOT TAKEN 2.009 3.421 NOT TAKEN NOT TAKEN 3.455 NOT TAKEN 3.613 4.155 NOT TAKEN 2.485 3.939 3.860 NOT TAKEN NOT TAKEN 3.508 NOT TAKEN SPILLED 3.758 NOT TAKEN 3.763 3.587 3.788 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.517 2.884 3.752 3.747 NOT TAKEN 4.171 3.549 NOT TAKEN NOT TAKEN 3.255 NOT TAKEN 3.069 3.084 NOT TAKEN 3.259 3.887 0.913 NOT TAKEN NOT TAKEN 3.230 NOT TAKEN 4.002 3.611 NOT TAKEN 4.128 3.350 3.703 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.569 2.134 3.777 3.282 NOT TAKEN 4.289 3.434 NOT TAKEN NOT TAKEN 1.665 NOT TAKEN 3.587 4.162 NOT TAKEN 4.031 4.328 NOT TAKEN NOT TAKEN NOT TAKEN 3.481 NOT TAKEN 3.867 3.821 NOT TAKEN 4.204 3.574 3.757 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.468 NOT TAKEN 3.641 3.653 NOT TAKEN 4.078 3.524 NOT TAKEN NOT TAKEN NOT TAKEN 1429 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 47 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 4 - 100 PPM C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 4.010 4.047 6.322 NOT TAKEN 4.162 4.216 4.305 4.067 4.265 4.162 6.257 NOT TAKEN 4.341 4.533 4.601 4.280 3.747 3.782 5.008 NOT TAKEN 3.903 3.833 4.310 4.055 3.691 3.648 5.442 NOT TAKEN 3.676 3.688 4.098 3.610 3.648 3.668 4.784 NOT TAKEN 3.497 3.635 3.820 3.747 3.449 4.297 4.993 NOT TAKEN NOT TAKEN 3.321 3.615 3.357 3.213 3.788 4.932 NOT TAKEN NOT TAKEN 3.479 3.593 3.591 1.518 2.508 3.624 NOT TAKEN NOT TAKEN 3.537 3.301 3.265 2.256 2.658 SPILLED NOT TAKEN NOT TAKEN 0.052 3.544 3.661 2.672 SPILLED SPILLED NOT TAKEN NOT TAKEN 4.625 3.308 3.402 1430 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 6 - 100 PPM C91041 C91042 C91043 C91044 C91045 C91046 C91047 C91048 C91049 4.154 4.291 4.545 4.599 4.194 NOT TAKEN 4.673 4.361 4.116 4.322 4.217 4.691 5.054 4.347 NOT TAKEN 5.322 4.518 1.039 4.091 3.896 4.184 4.482 3.838 NOT TAKEN 4.210 4.195 NOT TAKEN 3.875 4.016 3.851 4.454 3.166 NOT TAKEN 4.723 4.183 NOT TAKEN 3.701 3.624 3.602 4.272 NOT TAKEN NOT TAKEN 4.571 4.043 NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 48 WEEK 68 WEEK 72 1431 ANIMAL NUMBER WEEK 36 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 6 - 100 PPM C91050 C91051 C91052 C91053 C91054 C91055 C91056 C91057 C91058 C91059 C91060 C91061 C91062 C91063 C91064 C91065 C91066 C91067 C91068 C91069 C91070 C91071 C91072 C91073 C91074 C91075 C91076 C91077 C91078 C91079 C91080 4.234 4.568 4.087 4.160 4.110 4.336 4.369 4.162 4.208 3.860 4.408 4.007 5.042 4.654 4.261 4.370 4.120 4.347 NOT TAKEN 4.439 4.362 3.985 4.184 3.895 4.426 4.330 4.123 4.852 4.161 4.014 4.576 4.707 4.602 4.131 4.278 4.180 4.008 SPILLED 4.475 4.500 4.209 4.579 4.260 4.530 4.531 4.547 4.519 4.192 4.290 NOT TAKEN 4.516 4.799 4.126 4.535 4.096 4.631 4.439 4.376 4.920 3.814 4.486 4.461 4.127 4.166 3.874 3.927 3.832 3.948 4.143 4.159 4.182 3.685 4.063 3.771 4.054 4.214 4.254 3.990 3.974 3.836 NOT TAKEN 3.817 4.630 3.748 4.230 3.911 4.575 4.164 3.956 4.459 3.792 4.037 4.148 3.991 4.318 3.760 4.044 3.652 3.658 3.974 4.378 3.862 3.994 4.056 3.872 4.130 4.143 3.919 3.807 3.934 3.741 NOT TAKEN 3.798 4.215 3.742 3.882 3.825 4.176 3.976 4.164 3.995 4.225 4.178 4.165 3.904 4.011 3.757 3.922 3.611 3.420 3.873 3.904 3.831 4.001 3.811 3.736 3.822 4.088 3.773 3.908 3.764 3.646 NOT TAKEN 3.516 3.916 3.509 3.767 3.737 3.885 3.620 3.965 3.761 3.967 3.894 4.130 Covance 6329-212 3M T-6316.1 PAGE: 49 WEEK 68 WEEK 72 1432 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 50 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 2 - 3 PPM C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 0.135 NOT TAKEN 0.221 0.159 0.179 0.139 0.194 NOT TAKEN 0.171 0.174 0.178 0.163 0.145 0.172 NOT TAKEN 0.164 0.171 0.192 0.215 0.153 NOT TAKEN 0.191 0.175 0.181 0.176 0.210 NOT TAKEN 0.184 0.218 0.183 0.172 0.152 0.176 NOT TAKEN 0.163 0.173 0.191 0.225 0.159 NOT TAKEN 0.183 0.150 0.146 0.163 0.194 NOT TAKEN 0.163 0.158 0.150 0.135 0.141 0.158 NOT TAKEN 0.149 0.163 0.161 0.171 0.098 NOT TAKEN 0.163 0.149 0.160 0.142 0.156 NOT TAKEN 0.157 0.155 0.146 0.139 0.134 0.179 NOT TAKEN 0.155 0.154 0.157 0.156 SPILLED NOT TAKEN 0.129 0.132 0.163 0.130 0.135 NOT TAKEN 0.152 0.148 0.146 0.144 0.126 0.144 NOT TAKEN 0.147 0.155 0.164 0.166 0.140 NOT TAKEN 0.142 0.095 0.137 0.135 0.117 NOT TAKEN 0.146 0.132 0.149 0.125 0.109 0.151 NOT TAKEN 0.137 0.112 0.144 0.146 0.123 NOT TAKEN 0.147 0.140 0.135 0.138 0.130 NOT TAKEN 0.146 0.138 0.120 0.128 0.133 0.095 NOT TAKEN 0.147 0.162 0.138 0.139 0.140 NOT TAKEN 0.120 0.131 0.146 0.121 0.124 NOT TAKEN 0.138 0.147 0.155 0.131 0.131 NOT TAKEN NOT TAKEN 0.104 0.134 0.132 0.151 0.136 NOT TAKEN 0.128 0.156 0.139 0.130 0.120 NOT TAKEN 0.136 0.150 0.105 0.117 0.119 NOT TAKEN NOT TAKEN 0.123 0.125 0.113 0.166 0.123 NOT TAKEN 0.130 0.152 0.116 0.119 0.118 NOT TAKEN 0.139 0.122 0.137 0.119 0.122 NOT TAKEN NOT TAKEN 0.181 0.138 0.105 0.195 1433 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 51 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 2 - 3 PPM C91210 C91211 C91212 C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 0.179 0.141 0.175 0.141 0.178 0.191 0.186 0.163 0.223 SPILLED 0.151 0.176 NOT TAKEN NOT TAKEN 0.203 0.177 0.144 0.151 0.176 0.167 NOT TAKEN 0.135 0.178 NOT TAKEN 0.119 SPILLED 0.233 NOT TAKEN 0.189 0.164 0.175 0.175 0.155 0.172 0.169 0.178 0.195 0.182 0.186 0.240 0.261 0.167 0.182 NOT TAKEN NOT TAKEN 0.227 0.179 0.165 0.157 0.184 0.181 NOT TAKEN 0.154 0.192 NOT TAKEN 0.138 0.255 0.234 NOT TAKEN 0.181 0.148 0.164 0.159 0.139 0.159 0.154 0.149 0.147 0.168 0.180 0.236 0.187 0.143 0.178 NOT TAKEN NOT TAKEN 0.181 0.152 0.150 0.142 0.176 0.165 NOT TAKEN 0.160 0.164 NOT TAKEN 0.138 0.207 0.211 NOT TAKEN 0.164 0.159 0.134 0.138 0.117 0.142 0.146 0.144 0.142 0.152 0.146 0.194 0.174 0.128 0.157 NOT TAKEN NOT TAKEN 0.142 0.162 0.146 0.137 0.161 0.161 NOT TAKEN 0.148 0.170 NOT TAKEN 0.127 0.189 0.197 NOT TAKEN 0.158 0.145 0.165 0.135 0.119 0.142 0.145 0.141 0.204 0.143 0.161 0.163 0.150 0.123 0.135 NOT TAKEN NOT TAKEN 0.165 0.158 0.146 0.133 0.163 0.159 NOT TAKEN 0.126 0.149 NOT TAKEN 0.113 0.156 0.189 NOT TAKEN 0.145 0.149 SPILLED 0.126 0.128 0.148 0.117 0.127 NOT TAKEN 0.147 0.140 0.204 0.147 0.122 0.129 NOT TAKEN NOT TAKEN 0.158 0.147 0.138 0.128 SPILLED 0.144 NOT TAKEN 0.134 0.158 NOT TAKEN 0.107 0.144 0.178 NOT TAKEN 0.146 0.132 0.116 0.141 0.112 0.143 0.127 0.136 NOT TAKEN 0.160 0.146 SPILLED 0.143 0.122 0.136 NOT TAKEN NOT TAKEN 0.143 0.151 0.141 0.116 0.175 0.137 NOT TAKEN 0.121 0.154 NOT TAKEN 0.108 0.160 0.170 NOT TAKEN 0.138 NOT TAKEN 0.139 0.126 0.123 0.002 0.115 0.139 NOT TAKEN 0.173 0.133 0.196 0.135 0.110 0.140 NOT TAKEN NOT TAKEN 0.132 0.135 0.137 0.124 0.163 0.144 NOT TAKEN 0.116 0.160 NOT TAKEN 0.063 0.148 0.164 NOT TAKEN 0.021 NOT TAKEN 0.141 0.122 0.129 NOT TAKEN 0.109 0.148 NOT TAKEN 0.178 NOT TAKEN SPILLED 0.155 0.121 0.134 NOT TAKEN NOT TAKEN 0.140 0.129 0.134 0.120 0.160 0.142 NOT TAKEN 0.115 0.159 NOT TAKEN NOT TAKEN 0.165 0.166 NOT TAKEN NOT TAKEN NOT TAKEN 0.156 0.124 0.131 NOT TAKEN 0.066 0.149 NOT TAKEN 0.173 NOT TAKEN SPILLED 0.138 0.129 0.132 NOT TAKEN NOT TAKEN 0.142 0.139 0.126 0.129 0.171 0.163 NOT TAKEN 0.114 0.158 NOT TAKEN NOT TAKEN 0.171 0.174 NOT TAKEN NOT TAKEN NOT TAKEN 0.150 1434 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 52 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 2 - 3 PPM C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 C91249 C91250 NOT TAKEN 0.264 0.275 0.136 0.160 SPILLED 0.176 NOT TAKEN 0.160 0.184 NOT TAKEN SPILLED 0.213 0.193 0.155 SPILLED 0.192 NOT TAKEN 0.162 0.187 GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 NOT TAKEN 1.441 1.728 1.684 1.639 1.817 1.753 1.670 1.636 1.511 1.816 1.858 1.622 1.670 1.736 1.961 1.741 1.533 NOT TAKEN 1.602 1.702 1.777 1.746 1.865 1.747 1.776 1.757 1.592 1.926 1.947 1.647 1.845 1.572 1.959 1.868 1.545 NOT TAKEN SPILLED 0.154 0.169 0.154 SPILLED 0.172 NOT TAKEN 0.145 0.161 NOT TAKEN 1.529 1.465 1.685 1.653 1.712 1.554 1.641 1.611 1.490 1.839 1.643 1.563 1.664 1.434 1.699 1.618 1.433 NOT TAKEN 0.183 0.170 0.164 0.150 SPILLED 0.152 NOT TAKEN 0.136 0.158 NOT TAKEN 1.394 1.474 1.538 1.544 1.542 1.540 1.424 1.285 1.385 1.752 1.694 1.466 1.634 1.649 1.631 1.638 1.361 NOT TAKEN SPILLED 0.133 0.172 0.128 SPILLED 0.148 NOT TAKEN 0.127 0.167 NOT TAKEN 1.307 1.341 1.508 1.478 1.480 1.479 1.295 1.254 1.296 1.612 1.654 1.387 1.501 1.521 1.506 1.564 1.349 NOT TAKEN 0.176 0.132 0.166 0.123 SPILLED 0.122 NOT TAKEN 0.125 0.169 NOT TAKEN 1.183 1.103 1.355 1.534 1.554 1.447 1.353 0.788 1.269 1.590 1.567 1.333 1.425 1.281 1.481 1.643 1.160 NOT TAKEN 0.182 0.134 0.164 0.128 SPILLED 0.131 NOT TAKEN 0.124 0.166 NOT TAKEN 1.035 1.224 1.353 1.421 1.484 1.353 1.394 0.941 1.257 1.516 1.518 1.298 1.371 1.495 1.485 1.863 1.261 NOT TAKEN SPILLED 0.150 0.118 0.122 0.161 0.079 NOT TAKEN 0.117 0.139 NOT TAKEN 1.135 1.317 1.444 1.295 1.426 1.344 1.307 NOT TAKEN 1.078 1.446 1.456 0.832 1.337 1.344 1.392 1.670 1.170 NOT TAKEN SPILLED 0.145 NOT TAKEN 0.119 0.169 0.122 NOT TAKEN 0.125 0.142 NOT TAKEN 1.186 1.291 1.442 1.346 1.510 1.179 1.371 NOT TAKEN 0.543 1.337 1.611 1.716 1.337 1.251 1.500 NOT TAKEN 1.255 NOT TAKEN 0.153 0.134 NOT TAKEN 0.132 SPILLED 0.075 NOT TAKEN 0.110 0.132 NOT TAKEN 1.153 1.238 1.356 1.375 1.629 1.240 1.320 NOT TAKEN 1.068 1.388 1.553 NOT TAKEN 1.304 1.329 1.488 NOT TAKEN 1.435 1435 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 53 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 3 - 3 0 PPM C91269 C91270 C91271 C91272 C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 1.548 1.861 1.834 NOT TAKEN 1.803 1.514 1.566 2.030 1.821 1.779 1.783 1.785 NOT TAKEN 1.578 1.678 1.829 NOT TAKEN 2.448 NOT TAKEN NOT TAKEN 1.785 2.119 NOT TAKEN 1.447 NOT TAKEN 1.580 1.528 1.846 2.111 1.930 NOT TAKEN 1.876 2.021 2.271 NOT TAKEN 1.826 1.604 1.667 2.059 1.795 1.799 1.777 1.846 NOT TAKEN 1.824 1.658 1.699 NOT TAKEN 2.337 NOT TAKEN NOT TAKEN 2.125 2.137 NOT TAKEN 1.676 NOT TAKEN 1.797 1.735 2.144 SPILLED 2.881 NOT TAKEN 1.622 1.745 1.954 NOT TAKEN 1.846 1.480 1.663 1.820 1.652 1.591 1.620 1.621 NOT TAKEN 1.476 1.606 1.731 NOT TAKEN 2.000 NOT TAKEN NOT TAKEN 1.606 1.971 NOT TAKEN 1.457 NOT TAKEN 1.632 1.646 1.933 1.681 2.346 NOT TAKEN 1.563 1.135 1.710 NOT TAKEN 1.700 1.392 1.360 1.834 1.561 1.441 1.497 1.505 NOT TAKEN 0.857 1.404 1.258 NOT TAKEN 1.808 NOT TAKEN NOT TAKEN 1.468 1.851 NOT TAKEN 1.388 NOT TAKEN 1.610 1.652 1.788 1.677 2.127 NOT TAKEN 1.514 1.769 1.657 NOT TAKEN 1.555 1.389 1.284 1.702 SPILLED 1.413 1.408 1.540 NOT TAKEN 0.144 1.418 1.362 NOT TAKEN 1.842 NOT TAKEN NOT TAKEN 1.326 1.857 NOT TAKEN 1.307 NOT TAKEN 1.200 1.512 1.722 1.538 SPILLED NOT TAKEN 1.577 1.407 1.608 NOT TAKEN 1.535 1.461 1.199 1.663 1.507 1.359 1.475 1.510 NOT TAKEN NOT TAKEN 1.374 1.380 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.108 0.902 NOT TAKEN 1.347 NOT TAKEN 1.480 1.352 1.641 1.623 SPILLED NOT TAKEN 1.366 NOT TAKEN 1.660 NOT TAKEN 1.426 1.298 1.066 1.685 1.542 1.355 SPILLED 1.456 NOT TAKEN NOT TAKEN 1.306 1.213 NOT TAKEN 1.677 NOT TAKEN NOT TAKEN 1.313 NOT TAKEN NOT TAKEN 1.305 NOT TAKEN 1.336 1.391 1.562 1.623 SPILLED NOT TAKEN 1.321 NOT TAKEN 1.472 NOT TAKEN 1.501 1.317 SPILLED 1.557 1.479 1.425 1.371 1.359 NOT TAKEN NOT TAKEN 1.114 1.240 NOT TAKEN 1.603 NOT TAKEN NOT TAKEN 1.964 NOT TAKEN NOT TAKEN 1.133 NOT TAKEN 1.278 1.364 1.612 1.410 1.641 NOT TAKEN 1.369 NOT TAKEN 1.630 NOT TAKEN 1.594 1.205 1.986 1.645 1.461 1.398 1.759 1.408 NOT TAKEN NOT TAKEN 1.224 1.457 NOT TAKEN 1.788 NOT TAKEN NOT TAKEN 1.350 NOT TAKEN NOT TAKEN 1.203 NOT TAKEN 1.446 1.264 1.629 1.419 1.595 NOT TAKEN 1.293 NOT TAKEN 1.297 NOT TAKEN 1.558 NOT TAKEN NOT TAKEN 1.642 NOT TAKEN 1.373 1.507 1.352 NOT TAKEN NOT TAKEN 1.282 1.296 NOT TAKEN 1.532 NOT TAKEN NOT TAKEN 2.053 NOT TAKEN NOT TAKEN 1.165 NOT TAKEN 1.608 1.366 1.723 1.456 1.769 NOT TAKEN 1436 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 54 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 3 - 3 0 PPM C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 1.681 1.295 1.796 1.338 NOT TAKEN 1.820 1.955 1.625 SPILLED 1.646 1.693 1.867 1.632 1.843 1.409 NOT TAKEN 1.782 2.076 1.702 2.500 1.727 1.847 GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 7.211 6.001 7.172 9.844 6.453 NOT TAKEN NOT TAKEN NOT TAKEN 6.521 6.415 6.599 NOT TAKEN 7.249 5.826 5.832 7.175 6.025 7.702 6.303 6.964 10.963 6.951 NOT TAKEN NOT TAKEN NOT TAKEN 6.549 6.408 7.370 NOT TAKEN 7.542 6.367 6.212 7.578 6.477 1.594 1.457 1.742 1.402 NOT TAKEN 1.677 2.018 1.486 2.205 1.591 1.719 6.279 5.618 6.342 7.828 6.298 NOT TAKEN NOT TAKEN NOT TAKEN 6.008 5.739 6.557 NOT TAKEN 6.994 5.740 5.384 6.624 5.511 1.500 1.390 1.716 1.296 NOT TAKEN 1.492 1.799 1.575 2.002 1.516 1.679 6.410 5.742 6.063 10.274 6.150 NOT TAKEN NOT TAKEN NOT TAKEN 5.570 5.748 6.092 NOT TAKEN 6.459 5.752 5.066 6.864 5.921 1.373 1.385 1.487 1.248 NOT TAKEN 1.511 1.666 1.411 1.785 1.409 1.532 5.969 5.387 5.660 7.273 5.927 NOT TAKEN NOT TAKEN NOT TAKEN 5.383 5.552 5.950 NOT TAKEN 5.753 5.599 4.426 6.597 5.750 1.393 1.289 0.777 1.221 NOT TAKEN 1.353 1.791 1.473 1.645 1.371 1.449 5.764 5.298 5.519 8.336 5.554 NOT TAKEN NOT TAKEN NOT TAKEN 5.418 5.362 5.479 NOT TAKEN NOT TAKEN 5.104 5.060 6.404 5.647 1.124 1.194 1.866 1.074 NOT TAKEN 1.245 1.746 1.474 1.450 1.296 1.417 5.863 5.369 5.323 SPILLED 5.356 NOT TAKEN NOT TAKEN NOT TAKEN 5.229 5.037 5.400 NOT TAKEN NOT TAKEN 5.342 5.296 5.970 5.495 1.055 1.203 1.949 1.252 NOT TAKEN 1.311 1.541 1.360 1.389 1.166 1.315 NOT TAKEN 5.274 4.902 SPILLED 5.289 NOT TAKEN NOT TAKEN NOT TAKEN 4.839 4.827 4.861 NOT TAKEN NOT TAKEN 5.640 5.146 5.568 5.260 1.193 1.311 1.386 1.203 NOT TAKEN 1.302 1.591 1.453 1.449 NOT TAKEN 1.392 NOT TAKEN 5.541 5.095 8.267 5.062 NOT TAKEN NOT TAKEN NOT TAKEN 4.951 4.959 5.552 NOT TAKEN NOT TAKEN 5.650 5.628 6.180 5.414 1.319 1.231 NOT TAKEN 1.068 NOT TAKEN 1.232 1.559 1.366 NOT TAKEN NOT TAKEN 1.351 NOT TAKEN 5.519 5.069 4.753 4.866 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.666 4.807 NOT TAKEN NOT TAKEN 5.628 5.088 5.517 5.857 1437 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 55 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 4 - 100 PPM C91328 C91329 C91330 C91331 C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 5.154 NOT TAKEN 6.295 5.410 6.400 NOT TAKEN 6.108 5.717 15.370 NOT TAKEN 5.288 6.436 5.244 6.502 6.086 6.233 5.680 NOT TAKEN 5.698 SPILLED 6.033 SPILLED NOT TAKEN 5.311 6.030 6.946 5.933 NOT TAKEN SPILLED 5.551 5.872 6.601 NOT TAKEN 6.903 5.850 7.012 NOT TAKEN 6.132 6.483 12.687 NOT TAKEN 6.185 6.571 6.134 6.975 6.316 6.475 5.997 NOT TAKEN 6.017 6.457 7.057 SPILLED NOT TAKEN 5.871 6.382 7.090 6.403 NOT TAKEN 8.078 5.870 7.275 6.455 NOT TAKEN 6.177 5.144 6.413 NOT TAKEN 5.301 5.724 SPILLED NOT TAKEN 4.772 5.694 5.027 5.625 5.357 5.758 5.793 NOT TAKEN 5.345 5.951 6.996 SPILLED NOT TAKEN 5.797 5.477 5.674 5.843 NOT TAKEN 7.435 5.242 6.226 6.140 NOT TAKEN 5.795 4.718 5.802 NOT TAKEN 5.095 5.438 SPILLED NOT TAKEN 5.542 5.728 5.716 6.078 5.297 5.957 3.641 NOT TAKEN 5.072 6.175 7.242 SPILLED NOT TAKEN 4.979 5.703 6.234 5.462 NOT TAKEN 6.591 5.205 6.296 5.575 NOT TAKEN 5.314 4.629 5.906 NOT TAKEN 4.675 5.029 SPILLED NOT TAKEN 5.219 5.459 5.641 6.028 5.369 5.818 4.441 NOT TAKEN 4.802 5.906 6.811 SPILLED NOT TAKEN 5.294 5.491 5.627 5.472 NOT TAKEN SPILLED 5.299 5.949 4.503 NOT TAKEN 5.672 4.533 5.846 NOT TAKEN 4.346 4.521 SPILLED NOT TAKEN 4.667 5.122 5.052 5.787 4.270 NOT TAKEN 4.556 NOT TAKEN 4.871 NOT TAKEN NOT TAKEN 8.024 NOT TAKEN 4.897 NOT TAKEN NOT TAKEN 5.332 NOT TAKEN 7.176 5.041 5.366 5.712 NOT TAKEN 5.234 4.468 SPILLED NOT TAKEN 4.096 5.054 SPILLED NOT TAKEN 5.103 5.014 5.113 5.976 4.831 NOT TAKEN 5.006 NOT TAKEN 4.451 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 5.045 NOT TAKEN NOT TAKEN 5.013 NOT TAKEN 6.710 5.155 5.379 NOT TAKEN NOT TAKEN 4.687 4.034 5.683 NOT TAKEN 4.244 4.839 SPILLED NOT TAKEN 5.083 5.310 4.416 5.518 5.106 NOT TAKEN 4.463 NOT TAKEN 4.135 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.640 NOT TAKEN NOT TAKEN 4.375 NOT TAKEN 5.539 5.263 4.992 NOT TAKEN NOT TAKEN 4.819 4.106 5.937 NOT TAKEN 4.429 4.823 9.520 NOT TAKEN 5.637 5.209 4.852 5.696 4.953 NOT TAKEN 4.802 NOT TAKEN 3.742 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.576 NOT TAKEN NOT TAKEN 4.673 NOT TAKEN 5.978 4.880 4.724 NOT TAKEN NOT TAKEN 4.553 3.885 5.650 NOT TAKEN 4.706 5.160 SPILLED NOT TAKEN 5.117 5.033 4.389 5.667 4.074 NOT TAKEN 4.629 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.681 NOT TAKEN NOT TAKEN 5.143 NOT TAKEN 5.211 5.334 5.166 1438 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 56 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 4 - 100 PPM C91359 C91360 C91361 C91362 C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 6.760 6.999 5.227 5.875 6.081 5.943 SPILLED 5.898 7.080 6.113 6.080 NOT TAKEN 6.038 5.429 6.868 5.487 5.692 5.986 6.223 5.794 NOT TAKEN 5.664 8.135 7.732 5.447 6.547 5.679 6.650 SPILLED 6.333 7.140 6.569 6.929 NOT TAKEN 6.465 5.821 7.308 5.912 6.397 6.344 5.755 6.385 NOT TAKEN 6.384 6.376 6.747 5.327 5.859 5.290 5.633 10.673 5.493 5.879 5.582 5.710 NOT TAKEN 5.804 4.939 6.479 5.341 5.847 5.807 4.945 5.172 NOT TAKEN 5.899 6.180 6.306 5.446 5.160 5.229 5.649 11.678 5.513 5.628 6.231 5.956 NOT TAKEN 7.947 5.191 6.317 5.137 6.034 5.785 5.580 5.597 NOT TAKEN 6.055 5.954 6.182 5.195 5.315 4.976 5.555 10.829 5.507 6.035 6.474 5.530 NOT TAKEN 5.582 4.677 6.720 5.303 5.732 5.536 5.360 5.592 NOT TAKEN 5.710 5.641 5.942 5.471 4.421 4.816 5.275 NOT TAKEN 5.116 NOT TAKEN NOT TAKEN 5.402 NOT TAKEN NOT TAKEN 4.531 5.749 4.447 5.173 5.400 5.239 6.696 NOT TAKEN 5.745 5.308 6.010 5.519 4.577 4.903 5.400 NOT TAKEN 5.114 NOT TAKEN NOT TAKEN 5.659 NOT TAKEN NOT TAKEN 4.814 5.684 4.340 5.452 5.250 4.536 6.604 NOT TAKEN 5.533 5.035 5.599 5.231 4.782 4.750 4.783 NOT TAKEN 4.471 NOT TAKEN NOT TAKEN 5.009 NOT TAKEN NOT TAKEN 4.318 5.033 4.405 4.991 5.184 4.082 7.931 NOT TAKEN 5.225 5.578 5.512 4.740 4.896 4.644 5.094 NOT TAKEN 4.659 NOT TAKEN NOT TAKEN 5.362 NOT TAKEN NOT TAKEN 4.621 0.106 4.457 5.646 3.368 4.227 7.810 NOT TAKEN 5.406 5.692 5.389 4.605 4.728 4.636 5.191 NOT TAKEN 4.885 NOT TAKEN NOT TAKEN 5.160 NOT TAKEN NOT TAKEN 4.406 NOT TAKEN 4.441 5.404 5.347 NOT TAKEN SPILLED NOT TAKEN 5.563 1439 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 6 - 100 PPM C91451 C91452 C91453 C91454 C91455 C91456 C91457 C91458 C91459 C91460 C91461 C91462 C91463 C91464 C91465 C91466 C91467 C91468 C91469 C91470 C91471 C91472 C91473 C91474 C91475 C91476 7.546 6.048 6.288 6.105 6.200 5.712 5.827 6.380 SPILLED 6.228 4.868 5.760 6.151 6.444 6.634 5.193 6.450 SPILLED 6.067 6.307 6.143 5.415 5.937 6.547 6.474 6.303 6.725 6.491 6.862 6.330 6.394 6.548 6.303 6.868 SPILLED 6.956 5.558 6.458 6.348 7.186 7.466 6.089 7.310 SPILLED 6.469 6.919 6.796 5.910 7.451 7.305 6.751 6.754 5.866 5.883 5.923 5.704 5.745 5.659 5.599 5.973 9.704 5.746 4.994 5.729 5.462 6.253 6.629 4.929 6.628 7.736 5.624 5.626 5.729 5.206 5.341 5.422 6.054 5.833 5.549 5.374 5.400 5.465 5.671 5.502 5.257 6.142 SPILLED 5.711 4.900 5.193 5.546 6.472 6.182 5.043 6.562 6.463 5.562 5.913 5.661 5.266 5.162 5.817 5.860 5.875 5.721 5.170 5.281 5.329 5.400 5.334 5.026 5.518 SPILLED 5.040 4.489 5.087 5.530 6.484 5.836 4.992 6.196 6.992 5.710 5.253 5.099 4.557 5.513 5.525 5.437 5.401 Covance 6329-212 3M T-6316.1 PAGE: 57 WEEK 68 WEEK 72 1440 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 6 - 100 PPM C91477 C91478 C91479 C91480 C91481 C91482 C91483 C91484 C91485 C91486 C91487 C91488 C91489 C91490 6.347 6.328 5.970 6.481 5.905 6.231 6.163 6.023 6.196 6.293 5.744 5.554 6.367 SPILLED 6.578 6.855 6.891 6.844 6.613 6.701 6.510 5.815 6.548 6.332 6.505 5.672 6.950 6.926 5.809 6.338 5.681 6.362 5.907 5.592 5.597 5.908 5.913 5.878 5.407 5.189 5.867 6.031 5.759 6.547 5.222 6.188 5.957 6.022 5.568 5.396 5.456 5.988 5.865 5.284 6.073 6.002 5.181 5.813 5.327 5.442 5.639 5.478 3.792 5.128 4.401 5.632 5.354 4.647 5.583 5.507 Covance 6329-212 3M T-6316.1 PAGE: 58 WEEK 68 WEEK 72 1441 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 2 - 3 PPM C90781 C90782 C90783 C90784 C90785 C90786 C90787 C90788 C90789 C90790 C90791 C90792 C90793 C90794 C90795 C90796 C90797 C90798 C90799 C90800 C90801 C90802 C90803 C90804 C90805 C90806 C90807 C90808 C90809 C90810 C90811 0.100 0.103 0.109 0.101 0.100 0.102 0.101 0.090 NOT TAKEN 0.072 0.111 NOT TAKEN 0.081 0.065 NOT TAKEN NOT TAKEN NOT TAKEN 0.112 0.111 0.104 0.100 0.089 0.070 0.097 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.101 SPILLED 0.105 0.099 0.094 0.111 0.107 0.103 0.111 0.104 0.088 NOT TAKEN 0.039 0.116 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.114 0.107 0.107 SPILLED 0.003 0.124 0.103 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.098 0.104 0.098 SPILLED 0.056 0.107 0.118 0.109 0.097 0.096 NOT TAKEN NOT TAKEN 0.112 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.108 0.101 NOT TAKEN 0.128 NOT TAKEN 0.191 0.095 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.097 0.100 0.094 0.092 0.106 0.094 0.110 0.096 0.093 0.082 NOT TAKEN NOT TAKEN 0.103 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.086 0.091 NOT TAKEN 0.112 NOT TAKEN 0.194 0.090 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.088 0.091 0.093 0.098 0.102 0.074 0.117 0.095 0.084 0.084 NOT TAKEN NOT TAKEN 0.105 NOT TAKEN NOT TAKEN 0.113 NOT TAKEN NOT TAKEN NOT TAKEN 0.085 0.098 NOT TAKEN 0.113 NOT TAKEN SPILLED 0.089 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.085 0.096 0.092 0.079 0.093 NOT TAKEN 0.106 NOT TAKEN 0.091 0.077 NOT TAKEN NOT TAKEN 0.101 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.090 0.094 NOT TAKEN 0.105 NOT TAKEN NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.136 0.088 0.088 0.097 0.114 0.092 NOT TAKEN 0.083 NOT TAKEN 0.064 0.077 NOT TAKEN NOT TAKEN 0.093 NOT TAKEN NOT TAKEN 0.094 NOT TAKEN NOT TAKEN NOT TAKEN 0.020 0.096 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.063 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.114 0.083 0.092 0.085 0.112 0.086 NOT TAKEN 0.026 NOT TAKEN 0.094 0.075 NOT TAKEN NOT TAKEN 0.092 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.090 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.051 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.079 0.090 Covance 6329-212 3M T-6316.1 PAGE: 59 1442 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 2 - 3 PPM C90812 C90813 C90814 C90815 C90816 C90817 C90818 C90819 C90820 C90821 C90822 C90823 C90824 C90825 C90826 C90827 C90828 C90829 C90830 C90831 C90832 C90833 C90834 C90835 C90836 C90837 C90838 C90839 C90840 0.107 NOT TAKEN NOT TAKEN 0.105 0.086 NOT TAKEN NOT TAKEN 0.089 NOT TAKEN 0.096 0.000 0.095 0.102 0.102 0.116 NOT TAKEN 0.112 0.097 0.102 NOT TAKEN 0.105 NOT TAKEN NOT TAKEN 0.116 NOT TAKEN NOT TAKEN 0.092 0.086 0.086 0.107 NOT TAKEN NOT TAKEN 0.100 0.097 NOT TAKEN NOT TAKEN 0.102 NOT TAKEN 0.096 NOT TAKEN 0.097 0.015 0.101 0.114 NOT TAKEN NOT TAKEN 0.088 0.102 NOT TAKEN 0.086 NOT TAKEN NOT TAKEN 0.119 NOT TAKEN NOT TAKEN 0.090 0.081 SPILLED 0.109 NOT TAKEN NOT TAKEN 0.097 0.027 NOT TAKEN NOT TAKEN 0.100 NOT TAKEN 0.098 NOT TAKEN 0.103 NOT TAKEN 0.105 0.121 NOT TAKEN NOT TAKEN 0.092 0.110 NOT TAKEN 0.112 NOT TAKEN NOT TAKEN 0.103 NOT TAKEN NOT TAKEN 0.091 0.119 SPILLED 0.104 NOT TAKEN NOT TAKEN 0.090 NOT TAKEN NOT TAKEN NOT TAKEN 0.087 NOT TAKEN 0.092 NOT TAKEN 0.087 NOT TAKEN 0.099 0.102 NOT TAKEN NOT TAKEN 0.082 0.090 NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 0.114 NOT TAKEN NOT TAKEN 0.086 SPILLED SPILLED 0.099 NOT TAKEN NOT TAKEN 0.095 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.091 NOT TAKEN 0.073 NOT TAKEN 0.066 NOT TAKEN NOT TAKEN NOT TAKEN 0.078 0.095 NOT TAKEN 0.080 NOT TAKEN NOT TAKEN 0.112 NOT TAKEN NOT TAKEN 0.079 0.080 SPILLED 0.084 NOT TAKEN NOT TAKEN 0.080 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.085 NOT TAKEN 0.086 NOT TAKEN 0.095 NOT TAKEN NOT TAKEN NOT TAKEN 0.077 0.082 NOT TAKEN 0.096 NOT TAKEN NOT TAKEN 0.100 NOT TAKEN NOT TAKEN 0.085 0.095 SPILLED 0.076 NOT TAKEN NOT TAKEN 0.063 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.089 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.087 0.088 NOT TAKEN 0.098 NOT TAKEN NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 0.086 NOT TAKEN SPILLED 0.078 NOT TAKEN NOT TAKEN 0.076 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.096 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.059 0.078 NOT TAKEN 0.090 NOT TAKEN NOT TAKEN 0.101 NOT TAKEN NOT TAKEN 0.083 NOT TAKEN SPILLED Covance 6329-212 3M T-6316.1 PAGE: 60 1443 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 3 - 3 0 PPM C90841 C90842 C90843 C90844 C90845 C90846 C90847 C90848 C90849 C90850 C90851 C90852 C90853 C90854 C90855 C90856 C90857 C90858 C90859 C90860 C90861 C90862 C90863 C90864 C90865 C90866 C90867 C90868 C90869 C90870 C90871 1.058 1.026 NOT TAKEN 1.173 0.644 NOT TAKEN 1.101 NOT TAKEN 0.669 1.025 1.065 NOT TAKEN NOT TAKEN 1.117 0.948 0.947 1.064 0.955 NOT TAKEN 1.306 NOT TAKEN NOT TAKEN NOT TAKEN 0.984 1.095 NOT TAKEN 1.324 NOT TAKEN 1.033 0.726 0.901 1.089 1.065 NOT TAKEN 1.105 NOT TAKEN NOT TAKEN 1.108 NOT TAKEN NOT TAKEN 1.066 1.021 NOT TAKEN NOT TAKEN 1.079 1.028 0.978 1.117 1.123 NOT TAKEN 1.249 NOT TAKEN NOT TAKEN NOT TAKEN 1.062 1.059 NOT TAKEN 1.404 NOT TAKEN 1.019 NOT TAKEN 1.081 0.975 1.071 NOT TAKEN 1.142 NOT TAKEN NOT TAKEN 0.871 NOT TAKEN NOT TAKEN 1.007 1.016 NOT TAKEN NOT TAKEN 1.046 0.956 1.009 1.212 1.000 NOT TAKEN 1.275 NOT TAKEN NOT TAKEN NOT TAKEN 1.048 1.068 NOT TAKEN 1.286 NOT TAKEN 1.050 NOT TAKEN 0.995 0.850 0.866 NOT TAKEN 1.055 NOT TAKEN NOT TAKEN 0.854 NOT TAKEN NOT TAKEN 0.995 0.895 NOT TAKEN NOT TAKEN 0.938 0.856 0.893 1.087 0.928 NOT TAKEN 1.112 NOT TAKEN NOT TAKEN NOT TAKEN 0.945 1.007 NOT TAKEN 1.231 NOT TAKEN 0.975 NOT TAKEN 0.928 0.664 0.904 NOT TAKEN 1.048 NOT TAKEN NOT TAKEN 0.748 NOT TAKEN NOT TAKEN 0.788 0.934 NOT TAKEN NOT TAKEN 0.887 0.889 0.899 1.061 0.800 NOT TAKEN 1.073 NOT TAKEN NOT TAKEN NOT TAKEN 0.943 0.967 NOT TAKEN 1.174 NOT TAKEN 0.892 NOT TAKEN 0.941 0.317 0.881 NOT TAKEN 1.009 NOT TAKEN NOT TAKEN 0.249 NOT TAKEN NOT TAKEN 0.613 0.966 NOT TAKEN NOT TAKEN 0.851 0.821 0.826 0.875 SPILLED NOT TAKEN 1.066 NOT TAKEN NOT TAKEN NOT TAKEN 0.872 0.861 NOT TAKEN 1.112 NOT TAKEN 0.816 NOT TAKEN 0.819 NOT TAKEN 0.949 NOT TAKEN 0.859 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.949 0.925 NOT TAKEN NOT TAKEN 0.868 0.840 0.735 0.654 1.111 NOT TAKEN 1.062 NOT TAKEN NOT TAKEN NOT TAKEN 0.956 0.875 NOT TAKEN 1.170 NOT TAKEN 0.770 NOT TAKEN 0.887 NOT TAKEN 0.994 NOT TAKEN 0.747 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.815 0.876 NOT TAKEN NOT TAKEN 0.617 0.865 0.815 NOT TAKEN 0.811 NOT TAKEN 1.119 NOT TAKEN NOT TAKEN NOT TAKEN 0.978 0.840 NOT TAKEN 1.192 NOT TAKEN 0.888 NOT TAKEN 0.889 Covance 6329-212 3M T-6316.1 PAGE: 61 1444 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 3 - 3 0 PPM C90872 C90873 C90874 C90875 C90876 C90877 C90878 C90879 C90880 C90881 C90882 C90883 C90884 C90885 C90886 C90887 C90888 C90889 C90890 C90891 C90892 C90893 C90894 C90895 C90896 C90897 C90898 C90899 C90900 1.112 NOT TAKEN NOT TAKEN 1.020 NOT TAKEN NOT TAKEN NOT TAKEN 0.788 NOT TAKEN 1.043 0.995 1.186 1.414 0.979 1.092 NOT TAKEN 0.983 1.063 NOT TAKEN NOT TAKEN 0.010 1.040 1.026 1.029 NOT TAKEN 1.008 NOT TAKEN 1.148 1.072 1.095 NOT TAKEN NOT TAKEN 1.017 NOT TAKEN NOT TAKEN NOT TAKEN 0.334 NOT TAKEN 1.017 1.059 1.139 SPILLED 0.902 0.869 NOT TAKEN 0.009 0.973 NOT TAKEN NOT TAKEN NOT TAKEN 1.047 0.981 1.065 NOT TAKEN 0.975 NOT TAKEN 1.104 1.095 1.150 NOT TAKEN NOT TAKEN 1.038 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.979 1.052 1.400 1.306 0.933 0.590 NOT TAKEN NOT TAKEN 1.106 NOT TAKEN NOT TAKEN NOT TAKEN 0.990 1.035 1.046 NOT TAKEN 1.022 NOT TAKEN 1.100 1.039 0.784 NOT TAKEN NOT TAKEN 0.973 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.974 0.954 1.106 1.169 0.782 0.670 NOT TAKEN NOT TAKEN 0.983 NOT TAKEN NOT TAKEN NOT TAKEN 0.939 0.881 0.954 NOT TAKEN 0.938 NOT TAKEN 1.134 0.976 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.962 0.986 1.042 1.279 0.966 NOT TAKEN NOT TAKEN NOT TAKEN 1.008 NOT TAKEN NOT TAKEN NOT TAKEN 0.965 0.872 0.905 NOT TAKEN 0.952 NOT TAKEN 1.032 1.027 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.858 0.876 1.188 1.091 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.905 NOT TAKEN NOT TAKEN NOT TAKEN 0.922 0.835 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.918 0.952 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.887 0.820 0.907 1.062 1.087 NOT TAKEN NOT TAKEN NOT TAKEN 0.835 NOT TAKEN NOT TAKEN NOT TAKEN 0.883 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.623 0.906 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.840 0.827 1.051 0.570 0.811 NOT TAKEN NOT TAKEN NOT TAKEN 0.839 NOT TAKEN NOT TAKEN NOT TAKEN 0.884 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.957 Covance 6329-212 3M T-6316.1 PAGE: 62 1445 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 4 - 100 PPM C90901 C90902 C90903 C90904 C90905 C90906 C90907 C90908 C90909 C90910 C90911 C90912 C90913 C90914 C90915 C90916 C90917 C90918 C90919 C90920 C90921 C90922 C90923 C90924 C90925 C90926 C90927 C90928 C90929 C90930 C90931 NOT TAKEN NOT TAKEN 3.400 3.836 NOT TAKEN 3.122 NOT TAKEN 3.597 NOT TAKEN 3.316 3.451 3.395 NOT TAKEN 3.430 3.585 3.836 NOT TAKEN NOT TAKEN 3.611 4.025 NOT TAKEN NOT TAKEN 4.148 NOT TAKEN 3.909 3.489 3.740 3.602 NOT TAKEN 3.512 3.694 NOT TAKEN NOT TAKEN 3.374 3.813 NOT TAKEN 3.437 NOT TAKEN 3.585 NOT TAKEN 3.473 3.723 2.930 NOT TAKEN 3.481 3.530 3.749 NOT TAKEN NOT TAKEN NOT TAKEN 4.026 NOT TAKEN NOT TAKEN 3.214 NOT TAKEN 3.712 3.449 3.693 3.776 NOT TAKEN 3.768 3.604 NOT TAKEN NOT TAKEN 3.591 3.708 NOT TAKEN 3.308 NOT TAKEN 3.829 NOT TAKEN 3.543 3.607 NOT TAKEN NOT TAKEN 3.239 3.366 3.580 NOT TAKEN NOT TAKEN NOT TAKEN 3.926 NOT TAKEN NOT TAKEN 1.397 NOT TAKEN 3.397 3.396 3.564 0.811 NOT TAKEN 3.758 3.005 NOT TAKEN NOT TAKEN 3.085 3.413 NOT TAKEN 3.340 NOT TAKEN 3.259 NOT TAKEN 3.033 3.274 NOT TAKEN NOT TAKEN 2.570 3.247 3.096 NOT TAKEN NOT TAKEN NOT TAKEN 3.313 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.998 2.700 3.373 1.802 NOT TAKEN 3.911 3.318 NOT TAKEN NOT TAKEN 3.023 3.122 NOT TAKEN 3.369 NOT TAKEN 3.152 NOT TAKEN 3.319 3.095 NOT TAKEN NOT TAKEN 2.256 3.072 3.211 NOT TAKEN NOT TAKEN NOT TAKEN 3.875 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.563 3.293 2.756 4.520 NOT TAKEN NOT TAKEN 3.275 NOT TAKEN NOT TAKEN 2.914 3.169 NOT TAKEN 3.254 NOT TAKEN SPILLED NOT TAKEN 2.284 3.002 NOT TAKEN NOT TAKEN 1.649 NOT TAKEN 2.961 NOT TAKEN NOT TAKEN NOT TAKEN 3.785 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.930 2.962 3.615 NOT TAKEN NOT TAKEN 2.572 NOT TAKEN NOT TAKEN 2.440 3.301 NOT TAKEN 2.042 NOT TAKEN 3.180 NOT TAKEN 3.206 3.229 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.973 NOT TAKEN NOT TAKEN NOT TAKEN 3.121 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 3.202 3.643 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.915 NOT TAKEN NOT TAKEN NOT TAKEN 2.873 NOT TAKEN 3.055 3.171 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.941 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 3.110 4.724 NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 63 1446 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 4 - 100 PPM C90932 C90933 C90934 C90935 C90936 C90937 C90938 C90939 C90940 C90941 C90942 C90943 C90944 C90945 C90946 C90947 C90948 C90949 C90950 C90951 C90952 C90953 C90954 C90955 C90956 C90957 C90958 C90959 C90960 C90961 C90962 NOT TAKEN 3.588 4.069 NOT TAKEN 4.115 4.219 NOT TAKEN NOT TAKEN NOT TAKEN 3.007 NOT TAKEN 3.157 3.687 NOT TAKEN 2.223 3.721 3.994 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.550 NOT TAKEN 3.471 3.868 NOT TAKEN 1.121 3.396 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.236 SPILLED NOT TAKEN SPILLED 4.148 NOT TAKEN NOT TAKEN NOT TAKEN 2.096 NOT TAKEN SPILLED 3.821 NOT TAKEN NOT TAKEN 3.393 4.342 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.469 NOT TAKEN 3.837 3.968 NOT TAKEN 3.261 3.681 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.219 3.928 NOT TAKEN 3.949 4.297 NOT TAKEN NOT TAKEN NOT TAKEN 1.865 NOT TAKEN 3.844 3.750 NOT TAKEN NOT TAKEN 3.697 3.796 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.383 NOT TAKEN 3.715 3.623 NOT TAKEN SPILLED 2.549 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.817 3.776 NOT TAKEN 3.713 3.765 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.059 3.576 NOT TAKEN NOT TAKEN 3.807 3.257 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.064 NOT TAKEN 3.311 3.346 NOT TAKEN 3.521 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.204 3.406 NOT TAKEN 4.001 3.415 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.284 NOT TAKEN NOT TAKEN 3.655 3.450 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.148 NOT TAKEN 3.385 3.081 NOT TAKEN 2.898 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.605 3.286 NOT TAKEN 3.589 3.342 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.284 NOT TAKEN NOT TAKEN 3.881 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.821 NOT TAKEN 2.903 2.835 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.223 2.523 NOT TAKEN 2.686 3.239 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.526 NOT TAKEN NOT TAKEN 4.149 3.386 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.009 NOT TAKEN 2.977 2.781 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 2.777 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.169 NOT TAKEN NOT TAKEN 4.503 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.023 NOT TAKEN 2.975 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 64 1447 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 4 - 100 PPM C90963 C90964 C90965 C90966 C90967 C90968 C90969 C90970 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 3.724 3.497 3.473 NOT TAKEN NOT TAKEN 4.972 NOT TAKEN NOT TAKEN SPILLED 3.958 3.508 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 1.781 3.228 3.585 NOT TAKEN NOT TAKEN 4.747 NOT TAKEN NOT TAKEN 3.413 NOT TAKEN 2.943 NOT TAKEN NOT TAKEN 4.533 NOT TAKEN NOT TAKEN 2.312 NOT TAKEN 3.231 NOT TAKEN NOT TAKEN 4.151 NOT TAKEN NOT TAKEN 2.419 NOT TAKEN 3.351 NOT TAKEN NOT TAKEN 4.503 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.424 NOT TAKEN NOT TAKEN 3.372 NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 65 1448 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 2 - 3 PPM C91191 C91192 C91193 C91194 C91195 C91196 C91197 C91198 C91199 C91200 C91201 C91202 C91203 C91204 C91205 C91206 C91207 C91208 C91209 0.118 NOT TAKEN 0.111 0.159 0.124 0.105 0.114 NOT TAKEN 0.125 0.143 0.143 0.125 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.144 NOT TAKEN NOT TAKEN 0.115 NOT TAKEN SPILLED SPILLED 0.122 0.018 0.129 NOT TAKEN 0.143 0.147 0.142 0.123 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.158 NOT TAKEN NOT TAKEN 0.112 NOT TAKEN 0.129 0.144 0.126 NOT TAKEN 0.126 NOT TAKEN 0.124 0.140 0.129 0.080 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.144 NOT TAKEN NOT TAKEN 0.115 NOT TAKEN 0.067 0.122 0.109 NOT TAKEN 0.124 NOT TAKEN 0.131 NOT TAKEN 0.135 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.135 NOT TAKEN NOT TAKEN 0.133 NOT TAKEN NOT TAKEN 0.130 0.078 NOT TAKEN 0.123 NOT TAKEN 0.079 NOT TAKEN 0.125 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.048 NOT TAKEN NOT TAKEN 0.075 NOT TAKEN NOT TAKEN 0.112 0.049 NOT TAKEN 0.109 NOT TAKEN 0.117 NOT TAKEN 0.089 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.144 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.060 NOT TAKEN NOT TAKEN 0.103 NOT TAKEN NOT TAKEN NOT TAKEN 0.064 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.133 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.107 NOT TAKEN NOT TAKEN NOT TAKEN 0.132 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.140 NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 66 1449 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 2 - 3 PPM C91210 C91211 C91212 C91213 C91214 C91215 C91216 C91217 C91218 C91219 C91220 C91221 C91222 C91223 C91224 C91225 C91226 C91227 C91228 C91229 C91230 C91231 C91232 C91233 C91234 C91235 C91236 C91237 C91238 C91239 C91240 NOT TAKEN 0.118 NOT TAKEN 0.122 0.145 NOT TAKEN 0.184 NOT TAKEN 0.196 0.162 0.140 0.132 NOT TAKEN NOT TAKEN 0.135 0.138 0.110 0.136 0.182 0.099 NOT TAKEN 0.117 0.153 NOT TAKEN NOT TAKEN SPILLED 0.166 NOT TAKEN NOT TAKEN NOT TAKEN 0.146 NOT TAKEN 0.128 NOT TAKEN NOT TAKEN 0.142 NOT TAKEN 0.167 NOT TAKEN 0.140 0.135 0.137 0.130 NOT TAKEN NOT TAKEN 0.136 0.053 0.087 0.124 SPILLED 0.181 NOT TAKEN 0.123 0.162 NOT TAKEN NOT TAKEN SPILLED 0.178 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.120 NOT TAKEN NOT TAKEN 0.111 NOT TAKEN SPILLED NOT TAKEN 0.073 0.072 0.123 0.126 NOT TAKEN NOT TAKEN 0.144 NOT TAKEN 0.174 0.116 0.169 0.118 NOT TAKEN 0.123 0.160 NOT TAKEN NOT TAKEN SPILLED 0.173 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.120 NOT TAKEN NOT TAKEN 0.132 NOT TAKEN SPILLED NOT TAKEN 0.119 NOT TAKEN 0.102 0.123 NOT TAKEN NOT TAKEN 0.134 NOT TAKEN NOT TAKEN 0.098 0.101 0.117 NOT TAKEN 0.089 0.163 NOT TAKEN NOT TAKEN 0.130 0.130 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.128 NOT TAKEN NOT TAKEN 0.142 NOT TAKEN 0.147 NOT TAKEN 0.145 NOT TAKEN 0.127 0.117 NOT TAKEN NOT TAKEN 0.101 NOT TAKEN NOT TAKEN 0.105 0.084 0.036 NOT TAKEN NOT TAKEN 0.155 NOT TAKEN NOT TAKEN 0.135 0.140 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.113 NOT TAKEN NOT TAKEN 0.105 NOT TAKEN 0.158 NOT TAKEN SPILLED NOT TAKEN 0.115 0.118 NOT TAKEN NOT TAKEN 0.114 NOT TAKEN NOT TAKEN 0.109 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.136 NOT TAKEN NOT TAKEN 0.129 0.131 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.107 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.160 NOT TAKEN NOT TAKEN NOT TAKEN 0.096 0.101 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.106 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.142 NOT TAKEN NOT TAKEN 0.114 0.125 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.099 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.160 NOT TAKEN NOT TAKEN NOT TAKEN 0.082 0.091 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.109 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.119 NOT TAKEN NOT TAKEN 0.116 0.112 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 67 1450 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 2 - 3 PPM C91241 C91242 C91243 C91244 C91245 C91246 C91247 C91248 C91249 C91250 NOT TAKEN SPILLED 0.147 NOT TAKEN 0.132 0.131 NOT TAKEN NOT TAKEN 0.122 NOT TAKEN NOT TAKEN SPILLED 0.143 NOT TAKEN 0.122 0.160 NOT TAKEN NOT TAKEN 0.117 NOT TAKEN GROUP: FEMALE 3 - 30 PPM C91251 C91252 C91253 C91254 C91255 C91256 C91257 C91258 C91259 C91260 C91261 C91262 C91263 C91264 C91265 C91266 C91267 C91268 NOT TAKEN 1.154 1.274 1.347 NOT TAKEN SPILLED 1.291 1.139 NOT TAKEN NOT TAKEN 1.283 1.410 NOT TAKEN 1.344 1.511 1.437 NOT TAKEN 1.187 NOT TAKEN 1.257 1.360 1.309 NOT TAKEN 1.699 1.319 1.558 NOT TAKEN NOT TAKEN 1.342 1.443 NOT TAKEN 1.634 1.362 1.279 NOT TAKEN 1.325 NOT TAKEN SPILLED 0.148 NOT TAKEN 0.124 0.115 NOT TAKEN NOT TAKEN 0.109 NOT TAKEN NOT TAKEN 1.165 1.350 1.405 NOT TAKEN 1.735 NOT TAKEN 0.137 NOT TAKEN NOT TAKEN 1.182 1.319 NOT TAKEN 1.478 1.360 1.332 NOT TAKEN 1.094 NOT TAKEN 0.141 0.123 NOT TAKEN 0.119 NOT TAKEN NOT TAKEN NOT TAKEN 0.094 NOT TAKEN NOT TAKEN 1.128 1.206 1.344 NOT TAKEN 1.542 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.034 0.014 NOT TAKEN 1.374 1.100 1.188 NOT TAKEN 1.224 NOT TAKEN 0.129 0.114 NOT TAKEN 0.079 NOT TAKEN NOT TAKEN NOT TAKEN 0.015 NOT TAKEN NOT TAKEN 1.158 1.025 1.156 NOT TAKEN 1.427 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.113 NOT TAKEN NOT TAKEN 1.305 0.480 1.085 NOT TAKEN 1.146 NOT TAKEN 0.131 0.111 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.022 NOT TAKEN NOT TAKEN 0.983 1.044 1.374 NOT TAKEN 1.103 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.851 NOT TAKEN NOT TAKEN 1.183 NOT TAKEN 1.044 NOT TAKEN 0.973 NOT TAKEN 0.120 0.090 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.933 0.994 1.197 NOT TAKEN 1.111 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.071 NOT TAKEN 0.496 NOT TAKEN NOT TAKEN NOT TAKEN 0.124 0.091 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.957 1.014 1.372 NOT TAKEN 1.268 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.816 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 68 1451 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 3 - 3 0 PPM C91269 C91270 C91271 C91272 C91273 C91274 C91275 C91276 C91277 C91278 C91279 C91280 C91281 C91282 C91283 C91284 C91285 C91286 C91287 C91288 C91289 C91290 C91291 C91292 C91293 C91294 C91295 C91296 C91297 C91298 C91299 0.509 NOT TAKEN 1.471 NOT TAKEN 1.616 NOT TAKEN NOT TAKEN 1.556 NOT TAKEN 1.427 1.403 1.414 NOT TAKEN NOT TAKEN 1.249 1.316 NOT TAKEN 1.592 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.170 NOT TAKEN 1.617 1.309 1.599 1.396 NOT TAKEN NOT TAKEN 0.485 NOT TAKEN 1.131 NOT TAKEN 1.211 NOT TAKEN NOT TAKEN 1.801 NOT TAKEN 1.357 SPILLED 1.399 NOT TAKEN NOT TAKEN 1.458 1.387 NOT TAKEN 1.571 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.140 NOT TAKEN 1.602 1.470 1.037 1.492 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.923 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.622 NOT TAKEN 1.326 SPILLED 1.344 NOT TAKEN NOT TAKEN 1.186 1.344 NOT TAKEN 1.603 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.201 NOT TAKEN 1.544 1.277 1.027 1.397 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.268 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.577 NOT TAKEN 1.208 1.532 1.317 NOT TAKEN NOT TAKEN 0.021 1.204 NOT TAKEN 1.308 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.037 NOT TAKEN SPILLED 1.158 NOT TAKEN 1.574 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.610 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.447 NOT TAKEN 1.312 SPILLED 1.332 NOT TAKEN NOT TAKEN SPILLED 1.234 NOT TAKEN 1.375 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.061 NOT TAKEN 1.452 1.311 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.574 NOT TAKEN 1.266 1.184 0.717 NOT TAKEN NOT TAKEN NOT TAKEN 1.107 NOT TAKEN 1.347 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.158 NOT TAKEN 1.388 1.146 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.033 1.186 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.303 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.939 NOT TAKEN 1.296 1.054 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.101 1.156 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.241 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.966 NOT TAKEN 1.171 0.688 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 69 1452 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 3 - 3 0 PPM C91300 C91301 C91302 C91303 C91304 C91305 C91306 C91307 C91308 C91309 C91310 1.609 1.111 NOT TAKEN 1.162 NOT TAKEN 1.226 1.590 1.518 NOT TAKEN NOT TAKEN 1.394 SPILLED 1.159 NOT TAKEN 1.148 NOT TAKEN 1.106 1.470 1.445 NOT TAKEN NOT TAKEN 1.432 GROUP: FEMALE 4 - 100 PPM C91311 C91312 C91313 C91314 C91315 C91316 C91317 C91318 C91319 C91320 C91321 C91322 C91323 C91324 C91325 C91326 C91327 NOT TAKEN 5.607 4.665 NOT TAKEN 5.272 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.179 4.895 NOT TAKEN NOT TAKEN 6.020 4.921 5.413 5.652 NOT TAKEN 5.508 4.980 NOT TAKEN 5.640 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.910 NOT TAKEN NOT TAKEN 5.883 5.638 4.964 5.630 NOT TAKEN 1.258 NOT TAKEN 1.057 NOT TAKEN 1.098 1.492 1.288 NOT TAKEN NOT TAKEN 1.448 NOT TAKEN 5.054 3.155 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.521 NOT TAKEN NOT TAKEN 5.576 4.780 5.509 5.666 NOT TAKEN 1.154 NOT TAKEN 0.904 NOT TAKEN NOT TAKEN 1.584 1.316 NOT TAKEN NOT TAKEN 1.340 NOT TAKEN 4.772 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.429 NOT TAKEN NOT TAKEN 4.321 4.607 NOT TAKEN 4.769 NOT TAKEN 0.988 NOT TAKEN 1.073 NOT TAKEN NOT TAKEN 1.327 1.158 NOT TAKEN NOT TAKEN 1.139 NOT TAKEN 4.349 NOT TAKEN NOT TAKEN 4.906 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.414 NOT TAKEN NOT TAKEN 5.181 4.234 NOT TAKEN 5.361 NOT TAKEN 0.927 NOT TAKEN 0.905 NOT TAKEN NOT TAKEN 1.243 1.036 NOT TAKEN NOT TAKEN 1.198 NOT TAKEN 1.204 NOT TAKEN NOT TAKEN 4.704 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.641 3.528 NOT TAKEN 4.677 NOT TAKEN 0.825 NOT TAKEN 0.805 NOT TAKEN NOT TAKEN 1.293 0.903 NOT TAKEN NOT TAKEN 1.214 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.552 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.919 2.077 NOT TAKEN 4.196 NOT TAKEN 0.919 NOT TAKEN 0.887 NOT TAKEN NOT TAKEN NOT TAKEN 0.663 NOT TAKEN NOT TAKEN 1.210 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.102 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.451 NOT TAKEN NOT TAKEN 3.803 Covance 6329-212 3M T-6316.1 PAGE: 70 1453 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 4 - 100 PPM C91328 C91329 C91330 C91331 C91332 C91333 C91334 C91335 C91336 C91337 C91338 C91339 C91340 C91341 C91342 C91343 C91344 C91345 C91346 C91347 C91348 C91349 C91350 C91351 C91352 C91353 C91354 C91355 C91356 C91357 C91358 NOT TAKEN NOT TAKEN 4.909 1.577 8.815 NOT TAKEN 4.748 4.938 SPILLED NOT TAKEN 5.327 5.381 4.894 5.767 4.815 NOT TAKEN 4.958 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.206 NOT TAKEN NOT TAKEN 5.533 NOT TAKEN 6.264 5.199 NOT TAKEN NOT TAKEN NOT TAKEN 3.707 NOT TAKEN SPILLED NOT TAKEN 4.963 5.278 SPILLED NOT TAKEN 5.224 5.762 5.416 5.314 4.582 NOT TAKEN 5.446 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.406 NOT TAKEN NOT TAKEN 5.472 NOT TAKEN 6.306 5.060 NOT TAKEN NOT TAKEN NOT TAKEN 2.034 NOT TAKEN 6.333 NOT TAKEN 4.469 5.079 SPILLED NOT TAKEN 5.631 5.057 4.897 5.778 4.907 NOT TAKEN 4.483 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.831 NOT TAKEN NOT TAKEN 5.711 NOT TAKEN 5.418 4.820 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.840 NOT TAKEN 4.447 4.675 SPILLED NOT TAKEN 5.341 4.533 4.774 5.539 3.818 NOT TAKEN 4.355 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.888 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.793 4.583 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.953 NOT TAKEN 3.777 4.467 9.324 NOT TAKEN 4.779 2.935 4.794 4.997 4.202 NOT TAKEN 3.786 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 1.996 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.616 4.261 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 5.772 NOT TAKEN 4.426 3.973 SPILLED NOT TAKEN 4.337 4.329 4.523 5.301 4.152 NOT TAKEN 2.612 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.568 4.472 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.228 NOT TAKEN 6.315 3.580 8.380 NOT TAKEN 4.655 NOT TAKEN 3.578 4.614 3.868 NOT TAKEN 3.636 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 5.619 4.291 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.554 NOT TAKEN NOT TAKEN 3.832 SPILLED NOT TAKEN 3.730 NOT TAKEN 6.725 5.326 3.815 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.469 4.049 NOT TAKEN Covance 6329-212 3M T-6316.1 PAGE: 71 1454 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 4 - 100 PPM C91359 C91360 C91361 C91362 C91363 C91364 C91365 C91366 C91367 C91368 C91369 C91370 C91371 C91372 C91373 C91374 C91375 C91376 C91377 C91378 C91379 C91380 5.387 5.745 5.029 4.507 4.608 4.923 NOT TAKEN 4.338 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.549 NOT TAKEN 4.887 5.701 5.011 NOT TAKEN 7.449 NOT TAKEN 5.492 NOT TAKEN 6.174 4.834 4.396 4.098 5.328 NOT TAKEN 4.485 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.796 NOT TAKEN 4.976 3.202 5.457 NOT TAKEN SPILLED NOT TAKEN 5.575 NOT TAKEN 5.689 4.611 4.629 4.900 4.681 NOT TAKEN 4.460 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.891 NOT TAKEN 4.945 NOT TAKEN 5.338 NOT TAKEN SPILLED NOT TAKEN 6.558 NOT TAKEN 4.839 4.119 4.187 4.413 4.436 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.053 NOT TAKEN 4.266 NOT TAKEN 4.796 NOT TAKEN 7.824 NOT TAKEN 5.383 NOT TAKEN 5.108 4.418 3.599 4.000 5.325 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4.206 NOT TAKEN 2.882 NOT TAKEN 4.909 NOT TAKEN 8.874 NOT TAKEN 5.884 NOT TAKEN 4.921 4.177 3.531 SPILLED 5.001 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.622 NOT TAKEN 1.436 NOT TAKEN 4.769 NOT TAKEN SPILLED NOT TAKEN 6.438 NOT TAKEN 4.791 4.324 3.279 NOT TAKEN 7.263 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.919 NOT TAKEN NOT TAKEN NOT TAKEN 5.007 NOT TAKEN 8.630 NOT TAKEN 6.054 NOT TAKEN 4.639 3.806 3.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 3.703 NOT TAKEN NOT TAKEN NOT TAKEN 4.461 NOT TAKEN 5.823 NOT TAKEN 6.587 Covance 6329-212 3M T-6316.1 PAGE: 72 1455 APPENDIX 5 In d iv id u a l C lin ic al H e m a to lo g y D a ta In d iv id u a l C lin ic al C h e m istry D a ta In d iv id u a l C lin ic al U rin a ly sis D a ta In d iv id u a l U rin e C h e m istry D a ta Covance 6329-212 3M T-6316.1 1456 Appendix 5 Individual Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/m L Group: 1 C90717 C90718 C90737 C90756 C90769 MEAN S.D. N Group: 5 C90971 C90972 C90988 C90996 C91017 MEAN S.D. N Dose Level: 0 8.08 8.58 7.91 8.39 8.37 15.4 15.5 14.5 15.6 15.3 8.27 .267 5 15.3 .44 5 Dose Level: 300 7.34 6.28 6.35 5.93 7.17 13.4 12.2 12.3 12.0 13.4 6.61 .609 5 12.7 .68 5 Dosage Unit: ppm 45.1 46.6 42.6 44.5 44.8 55.9 54.2 53.9 53.0 53.5 44.7 1.43 5 54.1 1.10 5 Dosage Unit: ppm 38.3 36.2 35.6 35.2 40.3 52.1 57.7 56.1 59.2 56.2 37.1 2.14 5 56.3 2.65 5 19.1 18.1 18.3 18.6 18.2 18.5 .40 5 18.2 19.4 19.5 20.3 18.6 19.2 .82 5 34.1 33.4 34.0 35.1 34.1 34.1 .61 5 34.9 33.6 34.7 34.2 33.2 34.1 .72 5 1302 1300 1407 1106 1229 1269 111 5 1279 1042 989 1042 1106 1092 112 5 Covance 6329-212 3M T-6316.1 1457 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103//L X103//L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 1 Dose Level : 0 Dosage Unit: ppm C90717 C90718 C90737 C90756 C90769 7.9 1.1 5.9 .8 .1 .0 14 74 11 5.4 .7 4.2 .4 .1 .0 13 79 7 14.1 1.0 13.0 .0 .1 .0 7 92 0 7.4 1.8 5.0 .5 .2 .0 24 68 6 7.9 1.3 6.2 .4 .1 .0 16 78 5 2 1 1 2 1 0 0 0 0 0 MEAN S.D. N 8.5 1.2 6.9 .4 .1 .0 15 78 6 1 0 3.27 .41 3.52 .29 .04 .00 6.1 8.8 4..0 .5 .0 55555555555 Group: 5 Dose Level : 300 Dosage Unit: ppm C90971 C90972 C90988 C90996 C91017 7.3 .9 6.2 .2 .1 .0 12 84 2 1 0 5.2 .6 4.1 .4 .0 .0 12 80 7 1 0 8.5 .9 7.1 .5 .1 .0 11 83 6 1 0 7.7 1.0 6.7 .0 .0 .0 12 87 01 0 3.7 1.4 2.1 .2 .0 .0 38 57 4 0 0 MEAN S.D. N 6.5 1.0 5.2 .3 .0 .0 17 78 4 1 0 1.98 .29 2.10 .19 .05 .00 11.7 12.1 2..9 .4 .0 55555555555 1458 Appendix 5 Individual Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C90717 C90718 C90737 C90756 C90769 Group: 5 C90971 C90972 C90988 C90996 C91017 Dose Level: 0 Dose Level: 300 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1459 Appendix 5 Individual Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/m L Group: 1 C91126 C91140 C91145 C91162 C91163 MEAN S.D. N Group: 5 C91393 C91394 C91404 C91415 C91438 MEAN S.D. N Dose Level: 0 8.18 8.11 7.56 7.46 7.85 14.7 15.2 14.4 14.3 15.1 7.83 .321 5 14.7 .40 5 Dose Level: 300 6.18 6.93 6.82 7.09 7.15 11.8 13.3 12.7 13.1 14.0 6.83 .388 5 13.0 .81 5 Dosage Unit: ppm 44.2 45.2 41.9 41.8 44.8 54.0 55.7 55.3 56.0 57.1 43.6 1.62 5 55.6 1.13 5 Dosage Unit: ppm 34.5 38.8 36.8 38.6 41.0 55.9 56.0 54.0 54.5 57.3 37.9 2.43 5 55.5 1.31 5 17.9 18.7 19.1 19.1 19.3 18.8 .56 5 19.0 19.3 18.6 18.4 19.5 19.0 .46 5 33.2 33.6 34.4 34.2 33.8 33.8 .48 5 34.0 34.4 34.5 33.8 34.1 34.2 .29 5 1027 1117 1205 1022 1199 1114 88 5 1077 1305 1049 1016 1102 1110 113 5 Covance 6329-212 3M T-6316.1 1460 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103//L X103//L BASO N-SEG% X103//L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 1 Dose Level : 0 Dosage Unit: ppm C91126 8.7 .4 8.0 .1 .1 .0 5 93 1 1 0 C91140 6.1 1.2 4.3 .3 .3 .0 20 71 4 5 0 C91145 11.6 .6 10.5 .4 .1 .0 5 90 3 1 0 C91162 4.0 .5 3.1 .3 .1 .0 14 78 7 2 0 C91163 8.0 .7 7.0 .3 .1 .0 8 87 3 1 0 MEAN S.D. N 7.7 .7 6.6 .3 .1 .0 10 84 4 2 0 2.85 .31 2.95 .11 .09 .00 6.5 9.1 2.2 1.7 0 55555555555 Group: 5 Dose Level : 300 Dosage Unit: ppm C91393 C91394 C91404 C91415 C91438 5.9 .5 5.2 .2 .0 .0 8 88 3 1 0 5.2 1.1 3.9 .1 .0 .0 21 76 2 1 0 4.9 .5 4.2 .1 .0 .0 9 87 3 0 0 6.8 .3 6.2 .3 .0 .0 4 91 4 1 0 6.3 .5 5.4 .3 .0 .0 8 86 5 1 0 MEAN S.D. N 5.8 .6 5.0 .2 .0 .0 10 86 3 1 0 .78 .30 .93 .10 .00 .00 6.4 5.7 1.1 .4 0 55555555555 1461 Appendix 5 Individual Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 C91126 C91140 C91145 C91162 C91163 Group: 5 C91393 C91394 C91404 C91415 C91438 Dose Level: 0 Dose Level: 300 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1462 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/^L Group: 1 C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 MEAN S.D. N Group: 2 C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 MEAN S.D. N Dose Level: 0 8.83 8.25 8.60 7.97 7.83 8.98 8.50 8.70 9.00 8.71 15.6 15.3 15.3 14.9 14.3 15.5 15.8 15.3 16.0 15.2 8.54 .403 10 15.3 .48 10 Dose Level: 3 8.49 8.95 9.02 8.71 8.26 9.02 8.41 8.81 8.92 8.66 15.0 16.2 15.8 16.2 15.7 16.0 15.0 15.7 15.5 15.2 8.72 .268 10 15.6 .45 10 Dosage Unit: ppm 45.8 44.9 45.9 45.2 42.5 46.3 46.1 46.4 47.5 46.5 51.9 54.4 53.4 56.7 54.2 51.5 54.3 53.3 52.8 53.3 45.7 1.34 10 53.6 1.46 10 Dosage Unit: ppm 44.4 48.3 47.7 47.6 46.2 47.0 45.7 46.6 47.2 45.6 52.3 53.9 52.8 54.7 55.9 52.1 54.3 52.9 53.0 52.7 46.6 1.17 10 53.5 1.21 10 17.6 18.6 17.7 18.7 18.2 17.2 18.6 17.6 17.7 17.4 17.9 .55 10 17.7 18.1 17.5 18.6 19.1 17.7 17.9 17.8 17.4 17.5 17.9 .54 10 34.0 34.1 33.2 32.9 33.6 33.4 34.3 33.1 33.6 32.7 33.5 .53 10 33.7 33.6 33.1 34.0 34.1 34.0 32.9 33.7 32.8 33.3 33.5 .47 10 1359 1257 1261 948 1312 1342 1081 1407 1061 1374 1240 156 10 1112 1164 1168 972 1214 1134 1115 1181 1196 1141 1140 67 10 Covance 6329-212 3M T-6316.1 1463 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 3 C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 MEAN S.D. N Group: 4 C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 MEAN S.D. N Dose Level: 30 8.86 8.57 8.56 8.66 8.06 7.96 8.13 8.64 8.53 7.34 15.2 15.3 15.6 15.6 14.5 14.8 14.7 15.6 15.6 13.4 8.33 .455 10 15.0 .71 10 Dose Level: 100 8.12 7.48 8.35 7.87 8.05 8.36 8.19 8.80 8.34 8.24 15.4 14.3 15.0 14.6 14.6 15.6 15.1 15.5 15.1 14.8 8.18 .347 10 15.0 .43 10 Dosage Unit: ppm 44.1 45.6 45.9 46.1 43.9 43.7 43.9 46.2 46.2 40.2 49.8 53.3 53.6 53.2 54.4 54.9 54.0 53.5 54.1 54.8 44.6 1.87 10 53.6 1.45 10 Dosage Unit: ppm 45.3 42.0 45.2 42.4 43.2 46.3 45.4 46.5 44.2 43.8 55.8 56.2 54.1 53.9 53.7 55.4 55.4 52.9 53.0 53.2 44.4 1.57 10 54.4 1.23 10 17.1 17.9 18.2 18.0 18.0 18.6 18.0 18.1 18.2 18.2 18.0 .38 10 19.0 19.2 18.0 18.6 18.1 18.7 18.5 17.6 18.1 17.9 18.4 .51 10 34.4 33.6 33.9 33.8 33.1 33.9 33.4 33.8 33.7 33.3 33.7 .37 10 34.1 34.1 33.2 34.5 33.7 33.8 33.4 33.3 34.2 33.7 33.8 .42 10 1092 1156 1254 1244 970 1194 1354 1233 1131 804 1143 158.3 10 1148 1167 1148 1262 1159 1119 938 1171 1147 1152 1141 80.6 10 Covance 6329-212 3m T-6316.1 1464 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103//L X103///L BASO N -SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 1 Dose Level:: 0 Dosage Unit: ppm C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 8.2 .8 6.6 .6 .2 .0 10 81 7 2 0 7.4 .9 5.7 .6 .2 .0 12 77 8 3 0 8.3 1.5 5.7 .9 .1 .0 18 69 11 2 0 8.2 1.0 6.3 .8 .1 .0 12 76 10 2 0 8.2 1.5 6.0 .5 .1 .0 18 74 6 2 0 6.8 1.1 4.8 .7 .1 .0 17 71 10 2 0 7.7 2.1 4.4 1.1 .1 .0 27 57 15 1 0 8.9 1.4 6.8 .7 .1 .0 15 76 7 1 0 5.1 1.1 3.4 .5 .1 .0 22 67 10 2 0 5.2 1.1 3.3 .7 .1 .0 21 64 13 2 0 MEAN S.D. N 7.4 1.2 5.3 .7 .1 .0 17 71 10 2 0 1.31 .38 1.27 .19 .04 .00 5..2 7..1 2..8 .6 .0 10 10 10 10 10 10 10 10 10 10 10 Group: 2 Dose Level:: 3 Dosage Unit: ppm C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 9.5 1.0 7.8 .6 .1 .0 11 82 6 5.7 1.0 4.1 .5 .1 .0 18 71 8 12.7 1.3 10.5 .7 .1 .0 10 83 6 7.7 1.4 5.6 .5 .1 .0 18 73 6 12.5 2.4 8.6 1.0 .4 .0 20 69 8 17.7 2.4 13.8 1.3 .1 .0 14 78 7 9.6 1.5 6.7 1.2 .1 .0 16 70 13 8.3 1.9 5.4 .8 .2 .0 23 65 9 9.9 1.3 8.0 .5 .2 .0 13 81 5 10.7 1.0 8.7 .7 .3 .0 9 82 6 1 2 1 2 3 1 2 3 2 2 0 0 0 0 0 0 0 0 0 0 MEAN S.D. N 10.4 1.5 7.9 .8 .2 .0 15 75 7 2 0 3.31 .54 2.79 .29 .11 .00 4..6 6..6 2..3 .7 .0 10 10 10 10 10 10 10 10 10 10 10 1465 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103///L X103///L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 3 Dose Level : 30 Dosage Unit: ppm C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 12.6 2.1 9.1 1.2 .2 .0 16 72 9 10.6 .9 8.8 .7 .1 .0 8 83 7 6.3 .8 4.7 .6 .1 .0 13 74 10 10.8 1.1 9.0 .6 .1 .0 10 83 6 9.6 1.2 7.3 .7 .3 .0 13 77 8 7.3 1.6 5.4 .2 .2 .0 22 73 3 8.5 1.0 6.5 .7 .2 .0 12 77 8 8.4 1.7 6.2 .4 .1 .0 20 74 5 8.2 1.6 5.5 .8 .2 .0 20 68 10 9.7 1.4 7.1 1.0 .1 .0 15 73 10 2 1 2 1 3 2 2 2 2 2 0 0 0 0 0 0 0 0 0 0 MEAN S.D. N 9.2 1.3 7.0 .7 .2 .0 15 75 8 2 0 1.85 .41 1.59 .28 .07 .00 4..6 4..7 2..4 .6 .0 10 10 10 10 10 10 10 10 10 10 10 Group: 4 Dose Level : 100 Dosage Unit: ppm C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 11.3 1.5 9.1 .6 .2 .0 13 80 5 7.9 1.3 5.9 .6 .1 .0 17 75 7 5.8 .7 4.3 .7 .1 .0 13 74 11 8.1 1.3 6.2 .5 .1 .0 16 77 6 4.7 .6 3.5 .5 .1 .0 12 75 11 8.2 1.2 6.2 .7 .1 .0 14 76 9 12.2 1.9 8.8 1.3 .2 .0 16 72 11 9.0 1.6 6.6 .6 .2 .0 18 73 6 7.9 1.3 6.0 .5 .1 .0 17 76 6 12.9 1.3 10.8 .7 .2 .0 10 83 5 2 1 2 1 1 1 1 2 1 1 0 0 0 0 0 0 0 0 0 0 MEAN S.D. N 8.8 1.3 6.7 .7 .1 .0 15 76 8 1 0 2.64 .39 2.23 .24 .05 .00 2..6 3..3 2..5 .5 .0 10 10 10 10 10 10 10 10 10 10 10 1466 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 Dose Level: 0 C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 - - Group: 2 Dose Level: 3 C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 3M T-6316.1 1467 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 Dose Level: 30 C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 - - Group: 4 Dose Level: 100 C9 0905 C9 0907 C9 0917 C9 0921 C9 0934 C9 0947 C9 0953 C9 0957 C9 0960 C9 0968 Dosage Unit: ppm ----------- Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1468 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/^L Group: 1 C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 MEAN S.D. N Group: 2 C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 MEAN S.D. N Dose Level: 0 8.10 7.63 9.08 8.20 7.45 8.33 8.09 8.14 7.85 8.62 15.6 15.3 16.6 15.3 14.6 15.9 14.7 15.2 15.1 16.3 8.15 .469 10 15.5 .65 10 Dose Level: 3 7.71 7.98 7.73 8.50 7.68 8.07 8.48 8.03 8.10 8.96 14.7 14.4 15.3 15.8 15.3 14.9 16.2 14.6 15.2 16.2 8.12 .411 10 15.3 .64 10 Dosage Unit: ppm 45.9 45.1 49.8 46.1 42.6 47.2 44.6 45.5 44.2 48.4 56.7 59.1 54.8 56.2 57.1 56.6 55.2 55.9 56.3 56.1 45.9 2.09 10 56.4 1.17 10 Dosage Unit: ppm 43.3 43.0 45.7 46.8 45.1 44.6 48.6 43.0 44.1 48.8 56.2 53.8 59.1 55.0 58.7 55.3 57.3 53.6 54.5 54.5 45.3 2.16 10 55.8 1.97 10 19.3 20.1 18.3 18.7 19.7 19.1 18.1 18.6 19.2 18.9 19.0 .61 10 19.1 18.1 19.8 18.5 19.9 18.4 19.1 18.2 18.7 18.1 18.8 .67 10 34.0 34.0 33.4 33.3 34.4 33.8 32.9 33.3 34.1 33.6 33.7 .46 10 34.0 33.5 33.4 33.7 33.9 33.4 33.4 34.0 34.4 33.2 33.7 .38 10 1223 1074 958 1034 960 1031 1203 987 1079 846 1040 113 10 990 1105 865 1143 1086 1179 1139 1162 882 454 1000 222 10 Covance 6329-212 3M T-6316.1 1469 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/m L Group: 3 C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 MEAN S.D. N Group: 4 C91326 C91327 NR C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 MEAN S.D. N Dose Level: 30 8.66 8.19 8.30 7.81 7.51 7.55 7.48 8.48 7.91 8.17 15.9 15.9 15.6 14.9 15.5 14.6 14.9 15.6 14.3 15.3 8.01 .419 10 15.2 .55 10 Dose Level: 100 7.66 - 8.07 7.85 7.73 7.82 7.53 8.24 7.39 7.81 14.9 - 15.4 15.0 14.9 15.0 14.7 14.6 14.7 14.9 7.79 .258 9 14.9 .23 9 Dosage Unit: ppm 47.4 47.2 46.9 44.8 45.2 43.3 43.8 47.0 43.3 45.8 54.7 57.6 56.5 57.4 60.2 57.3 58.5 55.4 54.8 56.1 45.5 1.63 10 56.8 1.72 10 Dosage Unit: ppm 43.2 - 45.5 43.1 44.3 43.8 43.6 43.8 42.6 43.7 56.4 - 56.4 55.0 57.4 56.0 57.9 53.2 57.6 55.9 43.7 .82 9 56.2 1.46 9 18.4 19.5 18.8 19.1 20.7 19.4 19.9 18.4 18.0 18.8 19.1 .80 10 19.5 - 19.1 19.1 19.3 19.2 19.5 17.7 19.9 19.1 19.2 .61 9 33.6 33.8 33.2 33.2 34.4 33.8 34.0 33.2 32.9 33.5 33.6 .45 10 34.6 - 33.9 34.8 33.6 34.3 33.6 33.2 34.5 34.2 34.1 .54 9 1313 913 1112 1065 949 1044 903 1147 994 1097 1054 124 10 1035 - 1113 1017 1037 1016 994 968 1091 932 1023 56 9 Covance 6329-212 3m T-6316.1 1470 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103///L MONO EOSIN X103///L X103///L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 1 Dose Level : 0 Dosage Unit: ppm C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 5.5 .8 4.2 .4 .1 .0 14 75 7 3 0 7.2 1.2 5.6 .3 .1 .0 17 78 4 1 0 8.8 .7 7.4 .5 .2 .0 8 84 6 2 0 3.9 .6 3.0 .2 .1 .0 15 77 6 2 0 4.7 .6 3.9 .2 .1 .0 12 83 3 1 0 6.6 .6 5.4 .5 .1 .0 10 82 7 1 0 7.7 .7 6.3 .6 .1 .0 9 82 7 2 0 5.9 1.1 4.2 .4 .1 .0 19 71 7 2 0 5.6 .9 4.2 .4 .1 .0 16 75 7 2 0 8.6 1.0 6.9 .5 .2 .0 12 80 6 2 0 MEAN S.D. N 6.4 .8 5.1 .4 .1 .0 13 79 6 2 0 1.63 .22 1.44 .13 .04 .00 3..6 4..2 1..4 .6 .0 10 10 10 10 10 10 10 10 10 10 10 Group: 2 Dose Level : 3 Dosage Unit: ppm C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 5.0 .9 3.7 .3 .1 .0 18 74 6 2 0 5.7 .4 4.6 .4 .2 .0 8 82 7 3 0 8.1 1.4 5.9 .7 .1 .0 17 73 8 1 0 6.7 .5 5.7 .3 .1 .0 8 86 5 1 0 9.4 1.4 7.6 .3 .1 .0 15 81 3 1 0 5.4 .3 4.5 .4 .1 .0 6 84 8 2 0 9.2 1.1 7.5 .5 .1 .0 12 82 5 1 0 5.7 .7 4.3 .5 .1 .0 12 76 93 0 8.5 .7 7.2 .4 .1 .0 9 85 5 1 0 4.9 .2 4.2 .4 .1 .0 5 85 91 0 MEAN S.D. N 6.9 .8 5.5 .4 .1 .0 11 81 6 2 0 1.77 .43 1.48 .12 .03 .00 4..5 4..8 2..0 .8 .0 10 10 10 10 10 10 10 10 10 10 10 1471 ANIMAL NUMBER WBC X103//2L Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103//2L X103//2L MONO EOSIN X103//2L X103//2L BASO N-SEG% X103//2L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 3 Dose Level: 30 Dosage Unit: ppm C91262 5.8 .8 4.7 .2 .1 .0 13 80 4 2 0 C91274 22.5 1.2 20.2 .8 .4 .0 5 89 3 2 0 C91281 5.1 .6 4.1 .3 .1 .0 11 81 7 1 0 C91284 7.6 .7 6.3 .5 .1 .0 9 83 7 1 0 C91286 6.7 .5 5.8 .3 .1 .0 7 86 5 1 0 C91288 4.0 .6 3.0 .3 .1 .0 16 74 8 2 0 C91293 4.4 .8 3.3 .3 .1 .0 17 75 6 2 0 C91299 6.8 .7 5.6 .4 .1 .0 11 82 6 1 0 C91304 4.6 1.1 3.3 .1 .1 .0 24 70 3 2 0 C91305 7.8 .8 6.6 .3 .1 .0 10 85 4 2 0 MEAN S.D. N 7.5 .8 6.3 .4 .1 .0 12 80 5 2 0 5.43 .22 5.06 .19 .09 .00 5..5 5..9 1..8 .5 0 10 10 10 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C91326 4.7 .5 3.6 .5 .1 .0 10 77 11 2 0 C91327 NR - - - - - - - - - - - C91329 8.4 .5 7.3 .5 .1 .0 6 87 6 1 0 C91332 8.6 1.2 7.0 .2 .1 .0 14 82 2 1 0 C91337 6.1 .6 4.8 .4 .2 .0 10 79 7 3 0 C91348 8.1 .6 6.9 .4 .1 .0 8 85 5 2 0 C91355 4.2 .9 2.8 .5 .1 .0 22 65 11 2 0 C91370 6.1 .4 5.2 .4 .1 .0 7 85 6 1 0 C91374 5.5 .6 4.5 .3 .0 .0 12 81 6 1 0 C91379 4.0 .4 3.4 .2 .0 .0 11 83 4 1 0 MEAN S.D. N 6.2 .6 5.1 .4 .1 .0 11 80 6 2 0 1.80 .26 1.68 .12 .06 .00 4..8 6..6 3..0 .7 0 99999999999 1472 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 Dose Level: 0 C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 - - Group: 2 Dose Level: 3 C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 3M T-6316.1 1473 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 Dose Level: 30 C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 - - Group: 4 Dose Level: 0 C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 - QS - - QS - Dosage Unit: ppm ----------- Dosage Unit: ppm QS QS QS Covance 6329-212 3M T-6316.1 1474 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/m L Group: 1 C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 MEAN S.D. N Group: 2 C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 MEAN S.D. N Dose Level: 0 7.65 8.29 8.49 8.30 8.47 7.52 8.77 8.94 8.30 8.56 13.8 15.4 14.7 15.0 15.0 14.3 15.1 15.2 14.6 14.6 8.33 .445 10 14.8 .47 10 Dose Level: 3 8.88 7.52 9.11 8.34 9.16 8.48 8.13 7.96 8.19 6.17 16.1 13.9 15.3 14.6 16.1 14.5 15.3 14.6 15.1 11.0 8.19 .879 10 14.6 1.46 10 Dosage Unit: ppm 41.8 45.6 44.1 44.7 44.7 42.2 45.7 45.7 43.2 44.5 54.6 55.0 51.9 53.9 52.8 56.0 52.1 51.1 52.0 52.0 44.2 1.41 10 53.1 1.63 10 Dosage Unit: ppm 47.8 41.8 46.7 44.9 47.3 43.3 45.4 43.8 45.4 33.4 53.9 55.6 51.3 53.8 51.6 51.1 55.9 55.0 55.4 54.1 44.0 4.15 10 53.8 1.83 10 18.0 18.6 17.3 18.0 17.7 19.0 17.2 17.0 17.6 17.1 17.8 .66 10 18.1 18.4 16.8 17.5 17.5 17.0 18.8 18.3 18.5 17.9 17.9 .66 10 33.1 33.8 33.4 33.4 33.6 34.0 32.9 33.1 33.7 32.8 33.4 .40 10 33.7 33.2 32.7 32.5 34.0 33.4 33.6 33.4 33.4 33.1 33.3 .45 10 1345 1213 1132 1314 1172 1131 1234 1414 1334 1407 1270 107 10 1014 1018 1180 1023 1287 1148 1161 1135 1018 975 1096 100 10 Covance 6329-212 3M T-6316.1 1475 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 3 C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 MEAN S.D. N Group: 4 C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 MEAN S.D. N Dose Level: 30 8.19 8.47 9.08 8.32 8.59 8.16 7.80 7.87 8.04 8.20 14.5 14.4 15.6 14.6 15.0 15.5 14.0 15.0 15.3 15.1 8.27 .374 10 14.9 .51 10 Dose Level: 100 8.20 8.10 8.04 8.64 7.93 8.55 7.91 8.67 8.69 7.52 14.5 14.3 13.2 14.8 14.4 15.3 14.6 14.4 15.2 14.1 8.22 .398 10 14.5 .59 10 Dosage Unit: ppm 43.1 43.0 45.6 43.3 44.9 44.9 42.2 43.5 45.1 44.4 52.6 50.8 50.2 52.0 52.2 55.0 54.2 55.3 56.1 54.1 44.0 1.12 10 53.2 1.98 10 Dosage Unit: ppm 43.2 41.6 39.0 43.6 42.1 45.8 43.2 42.8 46.4 41.7 52.7 51.4 48.5 50.5 53.1 53.6 54.5 49.4 53.4 55.4 42.9 2.12 10 52.2 2.24 10 17.7 17.0 17.2 17.6 17.5 19.0 18.0 19.1 19.0 18.4 18.0 .78 10 17.7 17.6 16.4 17.1 18.2 17.8 18.5 16.6 17.5 18.8 17.6 .77 10 33.6 33.4 34.2 33.8 33.5 34.4 33.2 34.5 33.8 34.1 33.8 .44 10 33.6 34.3 33.9 33.9 34.2 33.3 33.9 33.5 32.8 33.9 33.7 .44 10 965 1322 1269 1277 1260 1011 1207 1057 1203 1326 1190 131.4 10 1142 1169 1185 1243 1327 1214 1202 1246 1193 1142 1206 55.6 10 Covance 6329-212 3M T-6316.1 1476 ANIMAL NUMBER WBC X103//7L Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103//7L X103//7L MONO EOSIN X103//7L X103//7L BASO N -SEG% X103//7L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 1 Dose Level:: 0 Dosage Unit: ppm C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 7.9 2.1 4.8 .9 .1 .0 26 61 11 7.3 1.7 4.8 .6 .2 .0 23 66 8 4.7 1.0 3.1 .4 .2 .0 22 65 9 7.2 1.3 4.9 .9 .1 .0 18 68 12 7.6 1.4 5.5 .6 .2 .0 18 72 8 8.8 1.4 6.5 .7 .1 .0 16 74 8 6.6 1.0 4.6 .8 .2 .0 15 69 12 11.6 1.4 8.9 1.0 .3 .0 12 77 8 8.6 1.3 6.0 1.0 .2 .0 16 70 12 8.4 1.8 5.6 .8 .2 .0 21 66 10 2 2 3 2 2 2 2 2 3 2 0 0 0 0 0 0 0 0 0 0 MEAN S.D. N 7.9 1.4 5.5 .8 .2 .0 19 69 10 2 0 1.77 .34 1.52 .19 .06 .00 4..2 4..7 1..8 .4 0 10 10 10 10 10 10 10 10 10 10 10 Group: 2 Dose Level:: 3 Dosage Unit: ppm C90795 6.5 1.3 4.1 1.0 .2 .0 20 62 15 3 0 C90799 7.7 1.2 6.0 .5 .1 .0 15 77 6 1 0 C90802 8.2 1.4 6.2 .4 .1 .0 17 76 5 1 0 C90803 7.6 1.5 5.0 .9 .2 .0 20 66 12 3 0 C90805 8.4 1.9 5.9 .5 .1 .0 22 70 6 2 0 C90806 6.4 .8 5.0 .4 .1 .0 13 79 6 2 0 C90824 8.4 1.0 6.3 .8 .3 .0 12 75 10 3 0 C90825 8.0 1.3 5.6 .9 .1 .0 16 70 12 2 1 C90839 6.0 .8 4.2 .8 .1 .0 13 71 14 2 0 C90840 11.8 3.0 8.0 .5 .3 .0 26 68 4 2 0 MEAN S.D. N 7.9 1.4 5.6 .7 .2 .0 17 71 92 0 1.62 .65 1.14 .23 .08 .00 4..5 5..3 4..1 .7 3 10 10 10 10 10 10 10 10 10 10 10 1477 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103///L MONO EOSIN X103///L X103///L BASO N -SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 3 Dose Level 30 Dosage Unit: ppm C90847 10.8 2.3 7.4 .8 .2 .0 22 69 7 1 0 C90855 10.3 3.8 5.6 .7 .2 .0 37 54 7 2 0 C90858 8.7 1.9 5.7 1.0 .1 .0 22 66 11 1 0 C90859 9.6 2.9 5.6 1.0 .1 .0 30 58 11 2 0 C90864 11.0 2.0 7.8 .9 .3 .0 18 71 8 3 0 C90865 9.1 1.1 6.9 .9 .1 .0 12 76 10 1 0 C90871 7.8 1.4 5.6 .6 .1 .0 18 72 8 1 0 C90872 5.9 1.0 4.4 .3 .1 .0 16 76 6 2 0 C90882 5.4 .7 4.1 .4 .2 .0 12 77 8 3 0 C90886 9.1 2.8 5.0 1.1 .1 .0 30 56 13 1 0 MEAN S.D. N 8.8 2.0 5.8 .8 .2 .0 22 68 92 0 1.91 .98 1.22 .27 .07 .00 8 3 8 7 2 2 .8 .0 10 10 10 10 10 10 10 10 10 10 10 Group: 4 Dose Level 100 Dosage Unit: ppm C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 8.1 2.0 5.4 .5 .1 .0 25 67 6 5.6 1.0 3.9 .6 .1 .0 17 70 11 7.7 2.1 4.7 .7 .1 .0 28 61 9 9.3 1.7 6.9 .6 .1 .0 18 74 7 9.9 1.6 7.5 .6 .1 .0 16 76 6 5.7 1.2 3.5 .8 .1 .0 22 62 14 8.6 1.1 6.5 .7 .2 .0 13 76 8 8.8 2.4 5.5 .8 .1 .0 27 62 9 8.4 1.8 5.6 .8 .2 .0 21 66 10 16.6 8.9 6.1 1.5 .1 .0 54 36 9 1 2 2 1 1 1 2 2 3 1 0 1 0 0 0 0 0 0 0 0 MEAN S.D. N 8.9 2.4 5.6 .8 .1 .0 24 65 92 0 3.06 2.34 1.27 .28 .04 .00 11 6 11 7 24 .7 .3 10 10 10 10 10 10 10 10 10 10 10 1478 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 Dose Level: 0 C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 - - Group: 2 Dose Level: 3 C9 0795 C9 0799 C9 0802 C9 0803 C9 0805 C9 0806 C9 0824 C9 0825 C9 0839 C9 0840 Dosage Unit: ppm ----------- Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1479 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 Dose Level: 30 C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 - - Group: 4 Dose Level: 100 C9 0916 C9 0931 C9 0937 C9 0940 C9 0944 C9 0945 C9 0954 C9 0956 C9 0957 C9 0963 Dosage Unit: ppm ----------- Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1480 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 1 C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 MEAN S.D. N Group: 2 C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 MEAN S.D. N Dose Level: 0 7.95 7.84 8.20 8.24 8.46 8.00 7.91 7.42 7.56 7.74 15.5 15.4 15.6 15.1 15.3 14.9 15.4 14.7 15.5 15.4 7.93 .316 10 15.3 .29 10 Dose Level: 3 7.55 7.80 7.24 7.59 7.27 7.84 7.87 7.39 7.27 7.60 14.5 14.3 14.6 14.8 14.4 15.5 15.3 14.8 15.0 13.8 7.54 .243 10 14.7 .50 10 Dosage Unit: ppm 45.5 45.3 46.7 44.7 46.2 44.1 45.3 43.0 45.3 44.8 57.3 57.7 57.0 54.3 54.5 55.1 57.2 57.9 59.9 58.0 45.1 1.04 10 56.9 1.76 10 Dosage Unit: ppm 42.2 42.5 43.2 44.1 42.4 45.1 45.2 42.8 43.5 41.5 55.8 54.5 59.7 58.1 58.4 57.6 57.5 58.0 59.8 54.6 43.2 1.23 10 57.4 1.88 10 19.5 19.6 19.0 18.4 18.1 18.6 19.5 19.8 20.5 20.0 19.3 .76 10 19.2 18.3 20.2 19.5 19.9 19.8 19.4 20.1 20.7 18.1 19.5 .82 10 34.0 34.0 33.4 33.8 33.2 33.8 34.0 34.3 34.2 34.4 33.9 .38 10 34.5 33.6 33.8 33.6 34.0 34.4 33.8 34.6 34.6 33.2 34.0 .49 10 1148 926 1078 994 1164 1098 1226 1000 981 1140 1076 96.6 10 1081 1029 858 1286 841 1135 1058 1024 891 665 987 176.1 10 Covance 6329-212 3M T-6316.1 1481 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106//2 L G/DL FL PG X103//2 L Group: 3 C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 MEAN S.D. N Group: 4 C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 MEAN S.D. N Dose Level: 30 7.82 7.71 7.71 7.16 7.94 7.25 7.58 8.37 8.32 7.96 14.4 14.5 15.3 14.1 15.0 15.1 14.5 15.0 15.5 15.2 7.78 .396 10 14.9 .46 10 Dose Level: 100 7.36 7.14 7.74 7.37 7.16 7.22 7.45 6.92 6.80 7.33 14.1 13.5 15.5 14.3 14.3 14.3 14.0 13.9 13.2 13.8 7.25 .268 10 14.1 .61 10 Dosage Unit: ppm 42.4 42.5 43.8 41.7 44.5 44.0 43.3 45.4 45.6 45.4 54.2 55.2 56.9 58.2 56.0 60.6 57.1 54.2 54.8 57.0 43.9 1.38 10 56.4 1.99 10 Dosage Unit: ppm 40.6 39.7 45.2 41.3 41.6 42.0 41.5 39.8 39.3 38.9 55.1 55.6 58.4 56.1 58.0 58.2 55.7 57.4 57.9 53.0 41.0 1.82 10 56.5 1.74 10 18.4 18.8 19.8 19.7 18.9 20.8 19.2 18.0 18.6 19.1 19.1 .80 10 19.1 18.8 20.1 19.4 19.9 19.8 18.9 20.0 19.5 18.8 19.4 .51 10 33.9 34.1 34.9 33.8 33.8 34.3 33.5 33.1 33.9 33.5 33.9 .49 10 34.7 33.9 34.4 34.6 34.4 34.1 33.8 34.9 33.7 35.5 34.4 .56 10 1010 1119 890 990 853 882 880 961 990 1109 968 94.2 10 1181 793 944 996 949 1046 1008 1043 922 1076 996 103.9 10 Covance 6329-212 3M T-6316.1 1482 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103//L X103///L MONO EOSIN X103///L X103///L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 1 Dose Level : 0 Dosage Unit: ppm C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 6.9 .6 5.9 .3 .1 .0 9 85 5 1 0 4.5 .4 3.6 .5 .1 .0 9 79 10 2 0 4.3 .8 2.3 1.0 .1 .1 19 54 23 2 2 3.9 .7 2.6 .4 .1 .0 19 67 11 3 0 3.4 .8 1.9 .6 .1 .0 23 57 17 2 1 6.2 .6 5.0 .5 .1 .0 9 81 8 2 0 5.0 .4 3.6 .8 .1 .0 8 73 16 2 1 3.7 .6 2.4 .6 .1 .0 15 65 16 3 0 6.3 .9 4.7 .6 .1 .0 14 74 10 2 0 6.4 .8 5.0 .4 .1 .0 13 78 7 2 0 MEAN S.D. N 5.1 .7 3.7 .6 .1 .0 14 71 12 2 0 1.29 .17 1.39 .21 .00 .03 5.2 10.3 5.5 .6 .7 10 10 10 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 3 Dosage Unit:: ppm C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 3.6 .4 2.8 .3 .1 .0 11 77 8 4 0 5.2 .9 3.6 .5 .1 .0 18 70 93 0 5.0 .5 3.8 .6 .1 .0 10 76 12 1 0 2.9 .5 2.0 .3 .1 .0 18 68 11 3 0 9.6 1.2 7.6 .6 .1 .0 13 79 6 1 0 6.5 .9 5.1 .2 .1 .0 15 79 4 2 0 5.4 .6 4.1 .6 .2 .0 11 76 10 3 0 3.9 .6 2.8 .4 .1 .0 16 73 92 0 4.9 .7 3.4 .7 .1 .0 14 69 15 2 0 5.1 .7 3.7 .6 .1 .0 13 73 12 2 1 MEAN S.D. N 5.2 .7 3.9 .5 .1 .0 14 74 10 2 0 1.85 .24 1.55 .17 .03 .00 2.8 4.0 3.2 .9 .3 10 10 10 10 10 10 10 10 10 10 10 1483 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103//L X103///L BASO N -SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 3 Dose Level : 30 Dosage Unit: ppm C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 4.0 .8 2.7 .4 .1 .0 20 68 93 0 6.6 1.3 4.2 .9 .1 .0 20 64 14 2 1 5.6 .8 4.0 .6 .1 .0 15 71 11 2 0 3.8 .8 2.6 .3 .2 .0 20 68 7 4 0 3.7 .8 2.1 .7 .1 .0 21 56 20 2 0 3.7 .4 3.0 .2 .1 .0 10 81 7 2 0 5.7 .8 4.0 .7 .1 .0 15 71 13 2 0 4.5 .8 3.1 .4 .1 .0 18 69 93 1 3.9 1.0 2.4 .5 .1 .0 25 61 12 2 0 6.6 .8 5.2 .4 .1 .0 12 79 7 2 0 MEAN S.D. N 4.8 .8 3.3 .5 .1 .0 18 69 11 2 0 1.20 .22 .98 .21 .03 .00 4 6 7 5 4 1 .7 .4 10 10 10 10 10 10 10 10 10 10 10 Group: 4 Dose Level : 100 Dosage Unit: ppm C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 5.3 1.3 3.4 .4 .1 .0 25 64 8 2 1 4.1 .9 2.8 .4 .1 .0 21 67 93 0 4.6 1.2 2.9 .4 .1 .0 26 64 8 3 0 5.7 .7 4.4 .4 .1 .0 13 77 8 2 0 4.4 .5 3.5 .3 .1 .0 12 79 6 2 0 3.9 .6 2.8 .4 .1 .0 14 72 11 2 0 6.0 .6 4.9 .3 .1 .0 11 82 5 2 0 4.4 .8 3.0 .5 .1 .0 18 68 12 2 1 5.2 .6 4.1 .3 .1 .0 12 79 6 2 0 5.0 1.1 3.4 .3 .2 .0 22 68 7 3 0 MEAN S.D. N 4.9 .8 3.5 .4 .1 .0 17 72 8 2 0 .69 .28 .73 .07 .03 .00 5 7 6 7 2 2 .5 .4 10 10 10 10 10 10 10 10 10 10 10 1484 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 Dose Level: 0 C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 - - Group: 2 Dose Level: 3 C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 3M T-6316.1 1485 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 Dose Level: 30 C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 - - Group: 4 Dose Level: 100 C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 3M T-6316.1 1486 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/m L Group: 1 C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 MEAN S.D. N Group: 2 C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 MEAN S.D. N Dose Level: 0 7.45 8.79 8.47 7.45 8.04 7.99 9.02 8.42 8.86 5.62 13.8 15.5 15.0 14.6 14.1 14.9 15.4 15.4 15.6 10.5 8.01 1.004 10 14.5 1.52 10 Dose Level: 3 8.01 8.87 8.99 9.10 7.61 8.73 8.48 8.33 7.94 8.14 14.8 16.3 16.1 15.6 14.3 15.6 15.8 14.8 14.6 14.2 8.42 .498 10 15.2 .76 10 Dosage Unit: ppm 41.6 46.6 44.6 43.2 41.8 44.4 46.0 46.7 46.4 31.7 55.8 53.0 52.6 58.0 51.9 55.5 51.0 55.5 52.4 56.5 43.3 4.50 10 54.2 2.32 10 Dosage Unit: ppm 43.8 48.6 48.3 46.3 41.8 46.6 48.4 45.2 42.6 41.3 54.7 54.8 53.7 50.9 54.9 53.3 57.1 54.2 53.7 50.8 45.3 2.79 10 53.8 1.88 10 18.6 17.6 17.7 19.7 17.6 18.7 17.1 18.3 17.6 18.7 18.2 .78 10 18.5 18.4 17.9 17.1 18.7 17.9 18.6 17.8 18.4 17.5 18.1 .52 10 33.2 33.2 33.5 33.9 33.8 33.7 33.4 33.0 33.6 33.2 33.4 .30 10 33.7 33.6 33.3 33.6 34.2 33.6 32.6 32.7 34.3 34.4 33.6 .61 10 1392 1226 1667 1006 1283 1180 1369 1074 1338 848 1238 229 10 1105 1247 1505 1286 942 1031 1264 1278 1046 1400 1210 176 10 Covance 6329-212 3M T-6316.1 1487 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 3 C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 MEAN S.D. N Group: 4 C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 MEAN S.D. N Dose Level: 30 8.65 8.74 7.03 9.31 8.48 8.25 8.52 8.09 8.60 8.26 14.5 15.0 11.9 16.0 15.2 14.8 15.0 15.0 14.5 15.8 8.39 .585 10 14.8 1.12 10 Dose Level: 100 8.58 7.89 8.69 7.61 8.68 8.46 8.27 8.30 8.96 9.08 15.0 14.5 14.7 13.1 15.1 14.3 14.4 14.8 15.5 15.3 8.45 .454 10 14.7 .68 10 Dosage Unit: ppm 44.2 44.7 35.3 47.3 45.1 44.5 44.1 44.4 43.1 45.2 51.1 51.1 50.2 50.8 53.2 53.9 51.7 54.8 50.1 54.7 43.8 3.17 10 52.2 1.82 10 Dosage Unit: ppm 44.0 42.1 43.5 39.8 44.8 43.2 41.9 43.2 45.2 45.5 51.3 53.4 50.0 52.3 51.6 51.1 50.7 52.0 50.5 50.1 43.3 1.73 10 51.3 1.06 10 16.8 17.2 16.9 17.2 18.0 18.0 17.6 18.5 16.8 19.2 17.6 .80 10 17.5 18.3 17.0 17.2 17.3 16.9 17.5 17.9 17.3 16.9 17.4 .45 10 32.9 33.7 33.7 33.8 33.8 33.3 34.0 33.8 33.6 35.1 33.8 .56 10 34.1 34.3 33.9 32.9 33.6 33.0 34.4 34.4 34.3 33.8 33.9 .55 10 1161 1201 1056 1328 1263 1190 1370 1182 1104 1133 1199 97.5 10 1015 1004 1367 831 1000 1173 1111 1450 1224 1118 1129 184.1 10 Covance 6329-212 3M T-6316.1 1488 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103//L X103///L BASO N -SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 1 Dose Level:: 0 Dosage Unit: ppm C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 7.1 2.6 3.5 .8 .1 .0 37 50 11 6.7 1.4 3.8 1.3 .2 .0 21 57 19 11.1 3.0 7.0 .8 .3 .0 27 63 8 6.9 1.9 3.9 .9 .2 .0 28 56 14 8.7 2.1 5.1 1.1 .3 .0 25 59 13 12.5 4.5 6.0 1.7 .2 .0 36 48 14 8.9 2.1 5.8 .8 .2 .0 24 65 9 8.2 2.5 4.7 .8 .1 .0 31 57 10 9.3 2.5 5.8 .9 .1 .0 27 62 10 5.4 1.6 3.2 .4 .1 .0 30 59 8 1 3 2 2 3 2 2 2 1 2 0 1 0 0 0 0 0 0 0 0 MEAN S.D. N 8.5 2.4 4.9 1.0 .2 .0 29 58 12 2 0 2.14 .88 1.26 .35 .08 .00 5..1 5..4 3..4 .7 .3 10 10 10 10 10 10 10 10 10 10 10 Group: 2 Dose Level:: 3 Dosage Unit: ppm C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 8.2 2.4 5.0 .6 .1 .0 30 61 8 1 0 8.4 1.8 5.4 .8 .2 .0 22 65 10 2 0 7.7 1.7 5.1 .7 .1 .0 22 66 92 0 9.1 3.6 4.5 .9 .2 .0 39 49 10 2 0 6.9 1.8 3.9 1.0 .1 .0 27 56 15 1 0 5.4 1.1 3.7 .5 .1 .0 21 68 91 0 8.1 1.3 5.8 .7 .2 .0 16 72 8 3 0 7.5 1.5 4.6 1.2 .2 .0 20 61 16 2 0 8.9 1.6 5.7 1.4 .1 .1 18 64 16 1 1 8.8 2.5 5.0 1.1 .1 .0 29 57 12 1 0 MEAN S.D. N 7.9 1.9 4.9 .9 .1 .0 24 62 11 2 0 1.11 .73 .70 .28 .05 .03 6..9 6..6 3..2 .7 .3 10 10 10 10 10 10 10 10 10 10 10 1489 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103///L MONO EOSIN X103///L X103//L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 3 Dose Level : 30 Dosage Unit: ppm C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 9.7 3.4 4.9 1.3 .2 .0 34 50 13 2 0 9.7 3.1 6.4 .1 .1 .0 32 66 1 2 0 8.5 2.8 5.0 .5 .1 .0 33 60 6 1 0 7.7 1.3 5.8 .5 .1 .0 17 75 7 1 0 8.8 1.5 6.1 1.0 .2 .0 17 69 11 2 0 8.2 2.8 4.3 1.0 .1 .0 34 53 12 2 0 9.8 1.4 7.2 1.0 .2 .0 15 73 10 2 0 9.8 2.9 5.8 .9 .3 .0 29 59 93 0 8.8 3.2 5.5 .0 .1 .0 36 63 01 0 7.4 2.6 3.8 .9 .1 .0 34 52 12 1 0 MEAN S.D. N 8.8 2.5 5.5 .7 .2 .0 28 62 8 2 0 .90 .79 1.01 .43 .07 .00 8..3 8..8 4..6 .7 .0 10 10 10 10 10 10 10 10 10 10 10 Group: 4 Dose Level : 100 Dosage Unit: ppm C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 6.9 1.5 4.1 1.1 .2 .0 22 60 16 6.2 1.6 3.9 .5 .1 .0 26 63 9 8.3 2.4 4.6 1.1 .2 .0 29 55 13 7.8 1.0 6.0 .7 .1 .0 13 77 8 7.9 1.5 5.3 .9 .1 .0 19 67 12 3.7 1.0 1.8 .7 .1 .0 29 50 18 9.5 2.3 6.0 .9 .2 .0 24 64 9 10.6 2.1 7.6 .8 .2 .0 20 72 7 8.5 2.3 5.2 .7 .2 .0 27 62 9 5.3 2.0 2.4 .7 .1 .0 39 46 13 2 2 2 1 1 2 2 2 3 2 0 0 0 0 0 0 0 0 0 1 MEAN S.D. N 7.5 1.8 4.7 .8 .2 .0 25 62 11 2 0 2.02 .53 1.74 .19 .05 .00 7..1 9..5 3..6 .6 .3 10 10 10 10 10 10 10 10 10 10 10 1490 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 Dose Level: 0 C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 1 - Group: 2 Dose Level: 3 C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 3M T-6316.1 1491 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 Dose Level: 30 C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 - - Group: 4 Dose Level: 100 C9 0908 C9 0914 C9 0923 C9 0926 C9 0927 C9 0934 C9 0936 C9 0944 C9 0946 C9 0956 Dosage Unit: ppm ----------- Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1492 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 1 C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 MEAN S.D. N Group: 2 C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 MEAN S.D. N Dose Level: 0 7.38 7.82 8.47 7.48 7.97 4.19 7.81 7.60 5.24 8.30 14.7 14.4 15.3 15.0 15.1 10.3 15.8 14.7 11.1 16.1 7.23 1.388 10 14.2 1.95 10 Dose Level: 3 7.17 7.31 8.20 8.04 7.28 7.37 6.76 7.81 7.90 6.32 13.3 14.6 15.1 15.6 14.2 14.4 14.1 15.2 14.2 12.0 7.42 .589 10 14.3 1.03 10 Dosage Unit: ppm 43.0 42.8 45.5 43.2 44.6 28.7 46.1 42.9 31.8 46.7 58.2 54.7 53.8 57.8 56.0 68.6 59.0 56.5 60.7 56.3 41.5 6.15 10 58.2 4.19 10 Dosage Unit: ppm 39.7 42.8 45.2 46.8 42.8 42.2 40.6 44.5 43.1 36.3 55.4 58.5 55.2 58.2 58.8 57.2 60.1 56.9 54.5 57.5 42.4 2.99 10 57.2 1.78 10 20.0 18.5 18.1 20.1 18.9 24.5 20.2 19.3 21.1 19.4 20.0 1.81 10 18.5 20.0 18.5 19.4 19.6 19.5 20.9 19.5 17.9 19.1 19.3 .85 10 34.3 33.7 33.7 34.7 33.8 35.8 34.2 34.2 34.8 34.5 34.4 .64 10 33.5 34.1 33.5 33.3 33.3 34.1 34.7 34.2 32.9 33.2 33.7 .56 10 851 870 1215 980 966 948 908 958 1113 1168 998 125. 10 1103 819 931 994 890 1012 1029 881 669 1019 935 125. 10 Covance 6329-212 3M T-6316.1 1493 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 3 C91255 C91258 C91264 C91269 C91270 C91274 C91289 C91294 C91297 C91305 MEAN S.D. N Group: 4 C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 MEAN S.D. N Dose Level: 30 7.97 8.01 7.52 7.49 4.77 7.23 8.13 7.91 7.79 7.60 15.3 15.7 15.3 13.9 9.8 14.7 16.9 14.5 14.9 14.8 7.44 .979 10 14.6 1.86 10 Dose Level: 100 6.14 7.40 7.56 8.03 6.98 4.55 6.35 7.46 7.50 7.23 13.0 14.5 14.8 15.1 13.6 9.6 12.6 14.0 14.6 14.3 6.92 1.008 10 13.6 1.62 10 Dosage Unit: ppm 44.6 46.2 43.9 41.0 28.6 42.1 50.1 43.4 44.5 43.8 56.0 57.7 58.4 54.7 59.9 58.2 61.6 54.9 57.1 57.7 42.8 5.57 10 57.6 2.13 10 Dosage Unit: ppm 37.6 41.9 42.6 44.0 40.0 27.3 37.2 40.1 42.2 40.7 61.2 56.6 56.3 54.8 57.4 59.9 58.5 53.8 56.3 56.3 39.4 4.74 10 57.1 2.24 10 19.2 19.6 20.4 18.5 20.5 20.3 20.7 18.4 19.1 19.5 19.6 .83 10 21.1 19.7 19.6 18.8 19.5 21.0 19.8 18.7 19.4 19.7 19.7 .79 10 34.4 34.0 34.9 33.8 34.2 34.8 33.7 33.5 33.5 33.7 34.0 .51 10 34.5 34.7 34.7 34.3 34.0 35.1 33.8 34.8 34.5 35.1 34.6 .43 10 937 1198 775 924 948 692 922 1007 875 897 918 133.9 10 773 868 765 977 903 935 936 974 979 962 907 80.9 10 Covance 6329-212 3M T-6316.1 1494 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N- ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103///L MONO EOSIN X103///L X103///L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 1 Dose Level : 0 Dosage Unit: ppm C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 4.0 1.0 2.4 .4 .1 .0 26 60 11 3 0 5.0 .9 3.2 .7 .1 .0 19 64 15 2 1 3.4 .5 2.3 .5 .1 .0 16 67 14 2 1 5.0 .9 3.4 .6 .1 .0 19 68 12 2 0 4.2 .7 2.8 .5 .1 .0 18 68 12 2 0 4.9 1.2 3.0 .6 .1 .0 24 61 13 2 0 4.9 1.7 2.4 .6 .1 .0 35 50 13 2 0 4.0 1.2 2.2 .5 .1 .0 30 54 12 3 0 5.2 1.7 2.6 .6 .2 .0 34 51 12 3 0 4.3 1.2 2.5 .5 .1 .0 27 58 12 2 0 MEAN S.D. N 4.5 1.1 2.7 .6 .1 .0 25 60 13 2 0 .59 .39 .40 .08 .03 .00 6.8 6.8 1.2 .5 .4 10 10 10 10 10 10 10 10 10 10 10 Group: 2 Dose Level: 3 Dosage Unit: ppm C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 5.3 1.2 3.4 .6 .1 .0 23 65 10 2 0 5.2 1.8 2.6 .6 .2 .0 34 50 11 4 0 4.2 .6 2.9 .6 .1 .0 14 70 14 1 0 3.2 .9 1.8 .4 .1 .0 28 57 13 2 0 6.4 1.4 4.2 .7 .1 .0 22 65 11 2 0 3.8 .6 2.8 .4 .1 .0 15 72 10 3 0 3.4 .8 2.1 .4 .1 .0 24 61 13 2 0 3.2 .7 2.0 .4 .1 .0 21 64 12 3 0 9.5 5.9 2.0 1.5 .1 .1 62 21 16 1 1 4.0 .8 2.7 .4 .1 .0 21 67 11 2 0 MEAN S.D. N 4.8 1.5 2.6 .6 .1 .0 26 59 12 2 0 1.95 1.60 .74 .34 .03 .03 13.8 14.8 1.9 .9 .3 10 10 10 10 10 10 10 10 10 10 10 1495 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103//L X103///L BASO N -SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% Group: 3 Dose Level:: 30 Dosage Unit: ppm C91255 7.0 1.5 4.7 .7 .1 .0 22 67 92 0 C91258 6.0 2.3 3.0 .6 .1 .0 38 49 11 2 0 C91264 4.0 .8 2.7 .5 .1 .0 19 67 12 3 0 C91269 3.9 1.0 2.4 .3 .1 .0 25 63 93 0 C91270 a 6.2 1.7 4.3 .1 .1 .0 28 69 2 1 0 C91274 7.6 1.2 5.8 .5 .1 .0 16 76 6 2 0 C91289 8.1 1.1 6.2 .7 .1 .0 13 76 91 0 C91294 4.0 .6 2.7 .4 .3 .0 16 66 11 6 0 C91297 4.1 1.0 2.3 .7 .1 .0 24 57 16 3 0 C91305 5.9 1.0 4.3 .4 .2 .0 17 73 6 3 0 MEAN S.D. N 5.7 1.2 3.8 .5 .1 .0 22 66 93 0 1.60 .49 1.43 .20 .07 .00 7..4 8..4 3..8 1.4 0 10 10 10 10 10 10 10 10 10 10 10 Group: 4 Dose Level:: 100 Dosage Unit: ppm C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 5.6 1.7 2.8 1.0 .1 .0 30 50 18 2 0 6.1 1.6 4.0 .3 .2 .0 26 66 4 4 0 4.1 1.2 2.2 .7 .0 .0 30 53 16 1 0 6.6 1.4 4.2 .8 .2 .0 21 65 12 3 0 5.2 1.6 2.8 .7 .1 .0 31 54 13 2 0 6.2 2.0 3.5 .5 .2 .0 32 56 8 3 1 6.0 1.2 3.9 .7 .1 .0 21 65 11 2 0 6.5 1.8 3.8 .7 .1 .0 27 60 11 2 0 6.3 2.2 3.4 .5 .2 .0 34 54 8 3 0 5.7 1.4 3.3 .7 .3 .0 24 58 12 5 0 MEAN S.D. N 5.8 1.6 3.4 .7 .2 .0 28 58 11 3 0 .74 .33 .63 .19 .08 .00 4..6 5..7 4..0 1.2 3 10 10 10 10 10 10 10 10 10 10 10 a Differential was read manually. 1496 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 1 Dose Level: 0 C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 1 - 1 Group: 2 Dose Level: 3 C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 - - Dosage Unit: ppm 1 Dosage Unit: ppm ----------- Covance 6329-212 3M T-6316.1 1497 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 3 Dose Level: 30 C91255 C91258 C91264 C91269 C91270 C91274 C91289 C91294 C91297 C91305 - - Group: 4 Dose Level: 100 C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 1 - 1 - Dosage Unit: ppm ----------- Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1498 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 1 C90719 C90725 C90732 C90753 C90775 MEAN S.D. N Group: 2 C90789 C90814 C90817 C90833 C90834 MEAN S.D. N Group: 3 C90846 C90853 C90866 C90890 C90891 MEAN S.D. N Dose Level 0 10 10 5 1 8 7 3.8 5 Dose Level 6 7 7 12 6 8 2.5 5 Dose Level 10 9 10 6 8 9 1.7 5 Dosage Unit: ppm Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1499 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 4 C90935 C90939 C90942 C90961 C90966 MEAN S.D. N Group: 5 C90984 C91005 C91024 C91033 C91037 MEAN S.D. N Dose Level: 100 6 9 5 4 6 6 1.9 5 Dose Level: 300 19 16 12 12 10 14 3.6 5 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1500 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 1 C91135 C91143 C91165 C91169 C91171 MEAN S.D. N Group: 2 C91198 C91205 C91222 C91230 C91237 MEAN S.D. N Group: 3 C91251 C91272 C91285 C91287 C91291 MEAN S.D. N Dose Level: 0 1 0 7 3 4 3 2.7 5 Dose Level: 3 3 7 4 6 6 5 1.6 5 Dose Level: 30 7 5 2 4 0 4 2.7 5 Dosage Unit: ppm Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1501 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 4 C91316 C91317 C91333 C91345 C91350 MEAN S.D. N Group: 5 C91423 C91427 C91430 C91443 C91448 MEAN S.D. N Dose Level: 100 7 3 4 6 4 5 1.6 5 Dose Level: 300 9 11 9 27 19 15 7.9 5 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1502 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 Dose Level : 0 Dosage Unit: ppm C90717 C90718 C90737 C90756 C90769 112 11 .7 6.4 4.4 2.0 .0 44 110 16 1.0 7.4 4.9 2.5 .1 74 105 12 .7 6.4 4.5 1.9 .1 51 108 13 .7 6.4 4.5 1.9 .2 40 96 13 .7 7.1 4.6 2.5 .2 57 MEAN S.D. N 106 13 .8 6.7 4.6 2.2 .1 53 6.3 1.9 .13 .48 .19 .31 .08 13.3 55555555 Group: 5 Dose Level : 300 Dosage Unit: ppm C90971 C90972 C90988 C90996 C91017 100 18 .6 7.0 5.5 1.5 .4 3 93 21 .7 6.7 5.4 1.3 .4 2 92 17 .8 6.4 4.9 1.5 .4 6 86 15 .7 7.2 5.7 1.5 .6 14 101 17 .6 8.0 5.9 2.1 .5 11 MEAN S.D. N 94 6.2 5 18 2.2 5 .7 7.1 5.5 1.6 .08 .61 .38 .30 5555 .5 .09 5 7 5.2 5 Covance 6329-212 3M T-6316.1 1503 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98 .1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C90717 C90718 C90737 C90756 C90769 MEAN S.D. N Group: 5 C90971 C90972 C90988 C90996 C91017 MEAN S.D. N Dose Level: 0 96 46 151 36 123 52 90 36 103 39 113 24.8 5 42 7.0 5 Dose Level: 300 166 219 256 633 169 250 104 67 202 187 179 55.6 5 271 213.8 5 Dosage Unit: ppm 3 10.7 4 10.7 1 10.4 3 10.5 0 10.2 2 10.5 1.6 .21 55 Dosage Unit: ppm 3 10.7 1 10.1 1 10.4 0 10.7 0 10.3 1 10.4 1.2 .26 55 8.3 6.6 8.6 8.1 7.5 7.8 .79 5 8.0 7.8 7.9 7.3 7.3 7.7 .34 5 147 148 146 150 148 148 1. 5 5 5.4 6.0 5.5 5.9 5.6 5.7 .26 5 148 146 149 147 145 147 1. 6 5 5.4 5.8 5.4 6.1 5.4 5.6 .32 5 104 102 101 106 104 103 1.9 5 103 106 104 103 100 103 2.2 5 Covance 6329-212 3M T-6316.1 1504 Appendix 5 Individual Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 1 C90717 C90718 C90737 C90756 C90769 MEAN S.D. N Group: 5 C90971 C90972 C90988 C90996 C91017 MEAN S.D. N Dose Level 10 3 12 11 9 9 3.5 5 Dose Level 28 29 33 22 20 26 5.3 5 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1505 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 Dose Level : 0 Dosage Unit: ppm C91126 C91140 C91145 C91162 C91163 107 17 115 11 107 13 97 13 120 12 .9 7.4 5.5 1.9 .8 7.1 5.4 1.7 .6 7.8 5.9 1.9 .8 7.0 5.2 1.8 .7 7.4 5.2 2.2 .3 53 .3 55 .3 66 .1 91 .3 79 MEAN S.D. N 109 13 .8 7.3 5.4 1.9 .3 69 8.8 2..3 .11 .31 .29 .19 .09 16.2 55555555 Group: 5 Dose Level : 300 Dosage Unit: ppm C91393 C91394 C91404 C91415 C91438 88 23 54 17 109 22 92 20 85 27 .8 6.5 5.5 1.0 .9 8.0 6.2 1.8 .7 7.5 5.9 1.6 .7 7.3 6.0 1.3 .9 9.2 7.2 2.0 .4 8 .4 7 .3 11 .5 16 .3 15 MEAN S.D. N 86 22 .8 7.7 6.2 1.5 .4 11 20.0 3..7 .10 1.00 .63 .40 .08 4.0 55555555 Covance 6329-212 3M T-6316.1 1506 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C91126 C91140 C91145 C91162 C91163 MEAN S.D. N Group: 5 C91393 C91394 C91404 C91415 C91438 MEAN S.D. N Dose Level: 0 131 38 115 31 119 33 115 31 166 77 129 21.6 5 42 19.8 5 Dose Level: 300 98 54 120 60 159 104 73 44 273 208 145 78.4 5 94 67.7 5 Dosage Unit: ppm 3 11.6 1 10.7 2 11.2 0 10.7 1 11.1 1 11.1 1.1 .38 55 Dosage Unit: ppm 0 10.9 1 11.3 2 10.9 0 10.9 4 11.8 1 11.2 1.7 .40 55 11.4 6.3 7.7 7.3 9.3 8.4 1.99 5 9.2 7.3 7.6 8.4 10.3 8.6 1.22 5 151 145 148 146 148 148 2.3 5 7.0 5.6 5.8 5.5 5.7 5.9 .61 5 148 147 146 150 153 149 2.8 5 5.7 5.4 5.7 5.2 6.1 5.6 .34 5 108 103 102 102 102 103 2 5 107 104 104 103 105 105 1 5 Covance 6329-212 3M T-6316.1 1507 Appendix 5 Individual Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 1 C91126 C91140 C91145 C91162 C91163 MEAN S.D. N Group: 5 C91393 C91394 C91404 C91415 C91438 MEAN S.D. N Dose Level: 0 3 0 2 7 5 3 2.7 5 Dose Level: 300 37 24 20 26 22 26 6.6 5 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1508 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 MEAN S.D. N Group: 2 C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 MEAN S.D. N Dose Level: 0 88 12 156 12 115 11 104 16 108 13 113 13 118 13 105 14 101 14 101 12 111 18.0 10 13 1. 4 10 Dose Level: 3 113 15 91 14 99 12 100 12 99 16 103 18 105 12 101 14 97 11 98 12 101 5.7 10 14 2. 2 10 Dosage Unit: ppm .6 7 .2 .7 7 .0 .6 7 .3 .6 7 .1 .6 7 .4 .6 8 .0 .6 7 .7 .8 7 .8 .6 7 .3 1.0 7 .3 .7 7 .4 .13 .32 10 10 Dosage Unit: ppm .7 6 .9 .7 7 .1 .6 7 .0 .7 7 .1 .8 7 .1 .7 6 .8 .7 6 .8 .8 7 .8 .7 7 .2 .7 7 .1 .7 7 .1 .06 .28 10 10 4.9 4.7 5.1 4.7 5.1 5.2 5.0 5.0 5.0 5.0 5.0 .16 10 4.5 4.7 4.8 4.9 4.8 4.6 4.6 4.9 4.9 4.7 4.7 .14 10 2.3 2.3 2.2 2.4 2.3 2.8 2.7 2.8 2.3 2.3 2.4 .23 10 2.4 2.4 2.2 2.2 2.3 2.2 2.2 2.9 2.3 2.4 2.4 .21 10 .3 112 .0 76 .1 78 .3 64 .2 91 .3 77 .1 64 .3 87 .2 92 .4 76 .2 82 .12 14.4 10 10 .3 56 .2 52 .1 57 .2 59 .1 60 .1 60 .0 68 .2 76 .1 62 .2 58 .2 61 .08 6.8 10 10 Covance 6329-212 3M T-6316.1 1509 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 Dose Level: 30 Dosage Unit: ppm C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 96 16 103 16 94 11 105 13 112 12 99 17 114 12 91 15 91 13 110 13 .7 7.8 4.9 2.9 .7 6.9 4.9 2.0 .7 6.9 4.6 2.3 .5 7.3 5.1 2.2 .6 7.4 4.6 2.8 .9 7.2 4.8 2.4 .5 6.7 4.5 2.2 .9 7.5 4.9 2.6 .6 6.8 4.7 2.1 .7 6.9 4.8 2.1 .0 44 .1 52 .3 55 .0 47 .0 70 .2 52 .1 64 .1 48 .2 52 .2 50 MEAN S.D. N 102 14 .7 7.1 4.8 2.4 .1 53 8.6 2.0 .14 .36 .18 .31 .10 7.9 10 10 10 10 10 10 10 10 Group: 4 Dose Level: 100 Dosage Unit: ppm C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 104 14 101 15 98 13 84 14 95 17 99 17 83 14 92 12 96 16 89 14 .7 7.3 5.5 1.8 .7 6.8 4.7 2.1 .6 7.5 5.1 2.4 .7 7.2 5.1 2.1 .6 7.2 5.3 1.9 .6 6.9 5.0 1.9 .6 7.8 5.3 2.5 .6 7.0 4.9 2.1 .7 7.6 5.2 2.4 .6 7.2 4.8 2.4 .2 12 .0 26 .2 27 .2 27 .2 38 .1 23 .2 47 .3 26 .2 24 .1 38 MEAN S.D. N 94 15 .6 7.2 5.1 2.2 .2 29 7.0 1.6 .05 .31 .25 .25 .08 9.8 10 10 10 10 10 10 10 10 Covance 6329-212 3M T-6316.1 1510 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 MEAN S.D. N Group: 2 C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 MEAN S.D. N Dose Level: 0 94 34 125 42 80 33 112 43 144 48 138 51 130 47 105 32 158 36 192 46 128 32.7 10 41 6. 9 10 Dose Level: 3 137 42 133 33 114 45 111 46 124 36 182 48 106 42 148 50 155 46 90 33 130 27.0 10 42 6. 1 10 Dosage Unit: ppm 0 10.7 0 10.6 0 11.0 0 10.7 1 10.6 1 11.2 0 11.2 0 10.5 0 11.1 0 10.0 0 .4 10 10.8 .37 10 Dosage Unit: ppm 0 10.5 1 10.1 0 10.8 1 10.9 0 10.7 0 10.3 0 10.7 2 10.4 0 10.6 0 11.0 0 .7 10 10.6 .28 10 8.0 7.9 7.7 8.2 7.0 8.0 8.7 7.0 7.7 6.7 7.7 .62 10 8.2 6.5 8.0 7.8 7.6 8.5 7.4 7.6 9.1 7.6 7.8 .69 10 147 150 149 148 147 150 147 150 152 149 149 1.7 10 6.2 6.2 6.8 6.1 6.4 7.5 6.4 6.8 6.4 6.6 6.5 .41 10 147 150 148 150 146 149 147 147 151 147 148 1.7 10 5.9 6.3 6.2 6.0 6.1 6.2 6.6 6.6 6.3 5.7 6.2 .28 10 104 105 107 105 105 105 105 104 108 105 105 1 10 106 106 103 108 101 105 103 101 107 105 104 2 10 Covance 6329-212 3M T-6316.1 1511 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 MEAN S.D. N Group: 4 C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 MEAN S.D. N Dose Level: 30 188 41 129 49 81 36 103 40 107 40 101 46 146 81 97 40 112 59 98 33 116 31.0 10 46 14.2 10 Dose Level: 100 116 52 72 39 81 49 145 51 121 59 80 33 111 30 157 48 150 66 78 46 111 32.3 10 47 11.1 10 Dosage Unit: ppm 1 11.0 0 10.8 0 10.5 0 10.9 0 11.0 0 10.7 0 10.3 0 10.9 0 10.4 0 11.1 0 .3 10 10.8 .28 10 Dosage Unit: ppm 4 10.7 0 10.3 0 10.9 0 9.9 0 10.5 0 10.8 0 11.0 0 10.5 0 10.2 1 10.8 0 1.3 10 10.6 .35 10 8.5 8.0 7.2 7.3 7.5 8.5 7.2 8.2 7.1 8.0 7.8 .55 10 8.2 8.1 7.9 7.5 7.8 7.3 7.5 8.2 7.0 7.3 7.7 .42 10 151 148 147 148 149 150 147 149 146 147 148 1.5 10 6.6 5.8 5.7 6.2 6.6 6.2 5.8 6.4 5.8 6.0 6.1 .34 10 150 151 148 148 147 147 147 147 151 147 148 1.7 10 5.8 6.3 6.4 6.1 6.2 6.1 5.6 6.3 6.7 5.6 6.1 .35 10 108 108 104 103 107 109 105 105 103 106 106 2.1 10 109 110 107 105 105 105 102 104 106 104 106 2.4 10 Covance 6329-212 3M T-6316.1 1512 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 MEAN S.D. N Group: 2 C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 MEAN S.D. N Dose Level: 0 105 12 128 10 118 13 105 15 102 13 109 13 97 14 92 15 108 13 100 14 106 10.4 10 13 1. 5 10 Dose Level: 3 91 13 112 12 103 17 110 12 108 17 113 16 109 12 121 11 108 13 102 12 108 7.9 10 14 2. 3 10 Dosage Unit: ppm .7 7 .4 .7 8 .3 1.0 8 .5 .7 9 .1 .7 8 .5 .9 8 .4 .7 8 .1 .9 8 .5 .9 8 .3 .9 7 .7 .8 8 .3 .12 .47 10 10 Dosage Unit: ppm .6 7 .3 .7 8 .0 .9 8 .1 .6 8 .1 .7 7 .5 .8 8 .3 .8 8 .3 .8 8 .4 .7 8 .4 .8 7 .7 .7 8 .0 .10 .39 10 10 5.5 5.8 6.1 6.9 6.3 6.3 5.9 6.6 5.9 5.7 6.1 .43 10 5.3 6.2 6.2 6.1 5.4 6.2 6.1 6.5 6.5 5.6 6.0 .43 10 1.9 2.5 2.4 2.2 2.2 2.1 2.2 1.9 2.4 2.0 2.2 .21 10 2.0 1.8 1.9 2.0 2.1 2.1 2.2 1.9 1.9 2.1 2.0 .12 10 .2 85 .3 157 .1 88 .3 129 .4 88 .2 51 .1 86 .3 104 .2 73 .1 86 .2 95 .10 29.6 10 10 .4 77 .0 61 .0 96 .3 95 .3 65 .2 91 .3 92 .1 71 .6 72 .3 81 .2 80 .18 12.9 10 10 Covance 6329-212 3M T-6316.1 1513 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 MEAN S.D. N Group: 4 C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 MEAN S.D. N Dose Level: 30 101 20 95 16 127 14 101 11 88 20 84 23 94 16 88 15 94 13 97 13 97 11.9 10 16 3.8 10 Dose Level: 100 98 24 91 25 93 15 89 18 107 18 105 24 104 14 97 19 85 14 75 15 94 9.9 10 19 4.3 10 Dosage Unit: ppm .7 7.7 .7 7.7 1.0 8.3 .7 8.3 .6 7.4 1.1 8.3 .7 6.6 .7 8.0 .8 8.1 .7 7.6 .8 7.8 .16 .53 10 10 Dosage Unit: ppm .7 8.0 .8 7.9 .7 8.1 .6 8.1 .7 8.2 .8 8.4 .8 8.8 .9 7.9 .5 8.3 .8 7.7 .7 8.1 .12 .31 10 10 5.8 5.6 6.2 6.0 5.7 6.2 5.0 5.7 5.9 5.4 5.8 .37 10 6.0 6.2 5.9 6.2 6.4 6.0 6.1 6.2 6.3 5.8 6.1 .19 10 1.9 2.1 2.1 2.3 1.7 2.1 1.6 2.3 2.2 2.2 2.0 .24 10 2.0 1.7 2.2 1.9 1.8 2.4 2.7 1.7 2.0 1.9 2.0 .32 10 .1 51 .2 86 .4 70 .2 60 .2 46 .1 101 .0 55 .0 57 .0 73 .1 89 .1 69 .13 18.3 10 10 .1 40 .1 33 .2 72 .4 44 .1 71 .1 75 .2 108 .1 42 .4 50 .2 48 .2 58 .12 22.9 10 10 Covance 6329-212 3M T-6316.1 1514 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 MEAN S.D. N Group: 2 C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 MEAN S.D. N Dose Level: 0 73 22 118 36 118 53 157 87 102 30 161 33 80 28 81 32 100 37 106 36 110 30.2 10 39 18.5 10 Dose Level: 3 115 26 72 27 98 28 88 43 103 33 93 36 75 41 103 27 116 36 92 25 96 14.8 10 32 6.5 10 Dosage Unit: ppm 0 11.4 0 11.6 0 11.8 0 11.0 3 10.8 1 11.3 0 11.0 0 11.9 0 11.5 0 11.1 0 1.0 10 11.3 .37 10 Dosage Unit: ppm 0 10.8 0 11.4 0 11.3 0 12.0 0 10.8 1 11.4 0 11.8 1 11.4 1 11.6 0 11.3 0 .5 10 11.4 .38 10 7.4 8.3 6.7 5.1 5.5 8.5 5.5 7.5 7.9 7.1 7.0 1.22 10 6.7 5.6 6.6 8.4 7.3 8.2 7.6 7.1 6.5 8.6 7.3 .95 10 146 151 153 148 147 151 146 148 150 147 149 2.4 10 6.3 6.5 7.3 5.4 4.8 6.7 5.4 6.3 5.9 5.0 6.0 .80 10 146 147 148 150 144 149 148 147 149 149 148 1.8 10 6.0 6.2 5.2 6.8 5.5 6.4 7.0 6.1 5.4 6.4 6.1 .59 10 107 108 108 107 105 107 104 104 107 102 106 2 10 104 106 105 104 104 105 104 103 105 107 105 1 10 Covance 6329-212 3M T-6316.1 1515 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 MEAN S.D. N Group: 4 C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 MEAN S.D. N Dose Level: 30 140 30 112 40 115 37 101 32 126 34 122 27 113 24 135 43 127 34 86 39 118 16.0 10 34 6.0 10 Dose Level: 100 70 31 75 35 76 27 74 34 76 26 93 31 95 40 85 39 105 37 124 34 87 17.1 10 33 4.7 10 Dosage Unit: ppm 0 10.6 1 11.1 0 11.4 0 10.9 1 10.7 1 11.2 0 9.9 0 11.0 0 10.2 0 11.0 0 .5 10 10.8 .46 10 Dosage Unit: ppm 2 10.8 0 10.8 3 11.3 0 11.4 0 11.3 4 11.3 2 11.4 1 11.3 0 11.1 0 10.7 1 1.5 10 11.1 .27 10 7.4 6.9 6.1 7.5 7.5 5.7 6.2 8.7 5.5 6.3 6.8 1.00 10 6.1 6.3 7.2 6.7 6.1 6.9 6.7 8.5 7.5 6.5 6.8 .74 10 151 145 152 150 146 149 150 148 148 148 149 2.2 10 6.0 5.3 5.9 5.9 5.8 6.4 5.9 5.8 5.2 6.0 5.8 .35 10 147 145 147 149 150 150 150 152 148 148 149 2.0 10 6.1 5.3 6.2 6.4 5.4 6.0 5.9 5.5 6.2 5.9 5.9 .37 10 107 103 105 105 106 105 108 107 105 106 106 1 10 105 104 105 106 109 104 108 110 106 104 106 2 10 Covance 6329-212 3M T-6316.1 1516 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 MEAN S.D. N Group: 2 C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 MEAN S.D. N Dose Level: 0 123 11 83 11 109 11 101 10 108 13 95 11 112 11 101 11 120 11 111 14 106 11.8 10 11 1.2 10 Dose Level: 3 95 9 105 12 102 12 123 14 115 12 91 13 98 13 109 13 114 12 128 16 108 12.1 10 13 1.8 10 Dosage Unit: ppm .6 7.4 .7 7.3 .4 6.9 .6 7.8 .5 7.4 .6 7.2 .6 7.4 .7 7.2 .7 7.0 .9 7.9 .6 7.4 .13 .31 10 10 Dosage Unit: ppm .7 7.7 .7 6.9 .7 7.1 .8 7.8 .6 7.6 .4 6.7 .6 7.8 .7 7.6 .6 7.5 .8 6.8 .7 7.4 .12 .43 10 10 4.6 4.6 4.2 5.1 4.7 4.5 4.6 4.4 4.5 4.9 4.6 .25 10 4.9 4.4 4.4 5.4 4.8 4.1 5.0 4.9 5.1 4.5 4.8 .39 10 2.8 2.7 2.7 2.7 2.7 2.7 2.8 2.8 2.5 3.0 2.7 .13 10 2.8 2.5 2.7 2.4 2.8 2.6 2.8 2.7 2.4 2.3 2.6 .19 10 .2 157 .0 73 .1 72 .1 95 .3 101 .3 75 .0 79 .0 122 .1 61 .3 124 .1 96 .13 30.3 10 10 .2 78 .2 95 .2 74 .2 112 .3 82 .3 58 .2 88 .1 106 .0 111 .0 59 .2 86 .11 19.8 10 10 Covance 6329-212 3M T-6316.1 1517 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 MEAN S.D. N Group: 4 C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 MEAN S.D. N Dose Level: 30 102 14 101 14 86 16 96 13 110 14 96 13 100 13 98 11 101 12 95 10 98 6.2 10 13 1.7 10 Dose Level: 100 97 14 97 13 98 14 96 13 100 18 92 14 86 15 79 16 104 16 99 14 95 7.3 10 15 1.6 10 Dosage Unit: ppm .7 6.7 .6 6.6 .8 8.0 .8 7.9 .7 6.9 .6 7.6 .8 6.8 .5 6.9 .6 7.4 .7 6.9 .7 7.2 .10 .51 10 10 Dosage Unit: ppm .6 7.4 .6 7.7 .7 7.3 .6 7.8 .6 8.1 .5 7.2 .6 7.6 .8 7.9 .4 7.9 .7 8.1 .6 7.7 .11 .32 10 10 4.4 4.1 5.2 5.1 4.3 5.2 4.6 4.5 4.8 4.4 4.7 .39 10 5.2 5.4 4.8 5.3 5.8 5.2 5.3 5.3 5.3 5.1 5.3 .25 10 2.3 2.5 2.8 2.8 2.6 2.4 2.2 2.4 2.6 2.5 2.5 .20 10 2.2 2.3 2.5 2.5 2.3 2.0 2.3 2.6 2.6 3.0 2.4 .28 10 .2 47 .2 42 .0 102 .0 67 .1 60 .1 64 .1 89 .1 86 .3 60 .0 68 .1 68 .10 18.8 10 10 .1 40 .0 35 .2 60 .0 43 .0 69 .2 19 .2 35 .0 42 .2 22 .3 59 .1 42 .11 16.2 10 10 Covance 6329-212 3M T-6316.1 1518 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 MEAN S.D. N Group: 2 C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 MEAN S.D. N Dose Level: 0 94 43 91 41 118 30 84 35 103 31 108 34 110 77 165 35 163 40 109 31 114 28.0 10 40 13.8 10 Dose Level: 3 99 37 89 33 117 47 215 101 121 43 142 30 130 37 97 33 96 31 87 33 119 38.3 10 42 21.2 10 Dosage Unit: ppm 0 11.4 1 11.3 1 10.3 4 10.8 1 10.6 0 10.5 2 11.2 3 10.7 0 10.5 1 10.6 1 1.3 10 10.8 .38 10 Dosage Unit: ppm 1 10.3 0 10.2 1 10.5 2 11.2 0 10.6 0 10.1 0 10.5 0 11.2 0 10.9 0 10.5 0 .7 10 10.6 .39 10 8.2 8.2 6.1 6.8 6.3 7.6 8.3 7.8 7.7 6.7 7.4 .82 10 6.0 6.3 7.0 7.5 6.8 6.7 7.0 7.7 7.9 6.1 6.9 .66 10 148 148 148 150 151 147 148 149 147 153 149 1.9 10 7.0 6.5 6.2 6.7 6.3 5.9 7.4 7.3 6.5 6.9 6.7 .48 10 146 146 146 152 147 147 143 148 147 148 147 2.3 10 5.8 6.0 6.5 7.0 6.3 6.2 5.4 6.8 6.2 5.8 6.2 .48 10 105 105 105 108 106 106 107 106 106 108 106 1 10 104 104 104 107 105 108 100 105 105 108 105 2 10 Covance 6329-212 3M T-6316.1 1519 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 MEAN S.D. N Group: 4 C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 MEAN S.D. N Dose Level: 30 84 52 82 48 123 58 116 37 142 83 84 36 209 136 90 40 102 39 108 44 114 38.7 10 57 31.0 10 Dose Level: 100 87 63 113 74 710 414 129 54 91 91 101 40 99 39 78 60 130 43 116 57 165 192.1 10 94 113.7 10 Dosage Unit: ppm 0 10.8 3 10.5 0 11.1 0 10.6 0 10.4 1 10.9 1 10.3 0 10.0 0 10.7 1 11.0 1 1.0 10 10.6 .34 10 Dosage Unit: ppm 1 10.1 0 10.9 2 10.4 1 10.9 0 10.7 0 10.4 0 11.1 1 10.7 2 10.8 1 11.1 1 .8 10 10.7 .32 10 7.2 7.3 7.1 7.0 6.7 7.2 7.2 6.8 7.2 8.2 7.2 .40 10 6.8 7.2 7.3 7.3 6.6 7.3 7.5 7.7 7.9 6.9 7.2 .40 10 148 146 148 148 146 149 147 145 146 145 147 1.4 10 6.7 5.7 6.5 6.1 6.4 6.5 6.1 5.9 6.7 6.8 6.3 .37 10 149 145 146 149 146 148 148 147 149 147 147 1.4 10 5.9 6.4 5.9 6.4 6.1 6.3 6.7 6.0 7.0 6.4 6.3 .35 10 109 106 105 105 106 105 107 106 105 106 106 1.2 10 108 105 107 105 105 107 105 106 106 105 106 1.1 10 Covance 6329-212 3M T-6316.1 1520 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 MEAN S.D. N Group: 2 C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 MEAN S.D. N Dose Level: 0 110 14 100 12 98 15 102 15 88 14 86 13 96 16 93 13 105 17 133 25 101 13.4 10 15 3.7 10 Dose Level: 3 118 15 99 15 109 13 101 13 107 14 100 17 104 19 93 12 95 13 105 13 103 7.3 10 14 2.2 10 Dosage Unit: ppm .5 8.1 1.0 7.9 .9 8.7 .7 7.7 .8 7.1 .6 8.4 .7 7.5 .4 7.8 .8 7.5 1.2 9.3 .8 8.0 .24 .65 10 10 Dosage Unit: ppm .6 8.2 .7 8.0 .7 8.4 .8 7.7 .7 7.4 1.0 7.9 .7 8.1 .6 8.2 .7 8.8 .7 9.3 .7 8.2 .11 .54 10 10 6.0 5.9 6.0 5.7 5.1 6.3 5.3 5.6 5.2 6.8 5.8 .53 10 6.1 5.8 6.4 5.3 4.8 5.2 5.7 6.1 6.1 6.6 5.8 .57 10 2.1 2.0 2.7 2.0 2.0 2.1 2.2 2.2 2.3 2.5 2.2 .23 10 2.1 2.2 2.0 2.4 2.6 2.7 2.4 2.1 2.7 2.7 2.4 .28 10 .3 82 .2 103 .2 93 .3 72 .2 100 .3 96 .0 75 .1 103 .2 62 .1 82 .2 87 .10 14.3 10 10 .1 76 .2 125 .2 110 .1 124 .0 121 .3 73 .4 91 .0 80 .4 115 .2 123 .2 104 .14 21.4 10 10 Covance 6329-212 3M T-6316.1 1521 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 MEAN S.D. N Group: 4 C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 MEAN S.D. N Dose Level: 30 104 13 91 20 99 16 111 14 121 11 101 23 116 19 114 14 108 16 107 14 107 8.9 10 16 3.7 10 Dose Level: 100 89 15 88 26 92 16 89 15 93 23 79 15 97 23 107 37 90 21 91 17 92 7.1 10 21 7.0 10 Dosage Unit: ppm .7 7.7 .7 7.3 .5 8.0 .6 8.8 .6 8.0 .7 7.3 .7 8.6 .5 9.0 .6 7.6 .6 7.6 .6 8.0 .08 .61 10 10 Dosage Unit: ppm .7 9.2 .8 9.1 1.0 9.2 .6 8.4 .9 8.4 .7 8.0 .9 8.1 1.3 8.3 .7 7.9 .7 8.8 .8 8.5 .21 .50 10 10 5.5 5.3 5.6 6.3 5.8 5.7 6.3 6.5 5.7 5.4 5.8 .41 10 6.7 6.6 6.4 6.1 6.0 6.0 5.9 6.2 5.8 6.2 6.2 .30 10 2.2 2.0 2.4 2.5 2.2 1.6 2.3 2.5 1.9 2.2 2.2 .28 10 2.5 2.5 2.8 2.3 2.4 2.0 2.2 2.1 2.1 2.6 2.4 .25 10 .2 78 .2 72 .2 95 .3 119 .3 80 .1 59 .1 90 .5 109 .2 93 .1 60 .2 86 .12 19.6 10 10 .2 58 .3 61 .4 75 .0 85 .3 41 .0 93 .2 77 .1 48 .1 61 .3 107 .2 71 .14 20.6 10 10 Covance 6329-212 3M T-6316.1 1522 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 MEAN S.D. N Group: 2 C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 MEAN S.D. N Dose Level: 0 102 39 101 23 97 31 101 45 183 47 116 40 109 30 141 64 110 31 164 88 122 30.0 10 44 19. 3 10 Dose Level: 3 75 30 80 37 104 43 70 30 153 83 109 29 100 28 110 28 109 48 105 57 102 23.6 10 41 17. 7 10 Dosage Unit: ppm 0 10.8 0 10.7 0 11.2 0 10.9 0 10.9 0 11.3 0 10.6 2 10.7 1 10.6 3 11.9 1 1.1 10 11.0 .41 10 Dosage Unit: ppm 1 11.0 1 11.0 0 11.7 0 10.9 15 10.7 0 10.5 0 10.6 0 11.2 0 11.0 1 11.7 2 4.7 10 11.0 .41 10 5.6 6.2 5.4 5.9 6.7 6.4 6.7 5.8 5.9 5.9 6.0 .44 10 4.7 5.4 7.1 6.4 6.4 5.0 5.4 6.2 5.8 5.1 5.8 .76 10 147 146 151 148 146 145 148 142 145 152 147 2. 9 10 5.6 5.4 6.1 6.1 6.5 5.8 7.1 5.5 6.3 6.8 6.1 .57 10 146 146 148 147 146 146 148 146 147 148 147 9 10 5.7 5.4 6.8 6.2 6.3 5.6 5.3 5.6 5.2 5.2 5.7 .54 10 108 104 105 107 106 102 107 102 106 105 105 2 10 104 106 106 107 106 106 104 103 105 103 105 1 10 Covance 6329-212 3M T-6316.1 1523 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 MEAN S.D. N Group: 4 C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 MEAN S.D. N Dose Level: 30 77 25 141 31 130 48 79 30 94 42 114 34 112 56 110 50 78 30 106 28 104 22.1 10 37 10.8 10 Dose Level: 100 61 26 122 69 52 26 78 42 92 34 144 59 91 52 112 36 104 48 197 76 105 42.4 10 47 17.3 10 Dosage Unit: ppm 2 10.7 0 10.8 3 10.8 0 11.1 1 11.1 1 10.4 3 11.4 1 12.3 0 10.7 4 10.8 2 1.4 10 11.0 .53 10 Dosage Unit: ppm 1 11.3 0 11.0 0 11.2 1 11.4 2 10.9 2 11.4 0 10.8 4 11.0 0 11.2 2 11.5 1 1.3 10 11.2 .24 10 5.2 6.1 6.1 4.7 6.1 6.3 6.8 8.0 5.3 6.3 6.1 .92 10 5.7 4.9 6.5 6.1 5.9 6.5 6.0 7.2 6.1 6.8 6.2 .63 10 146 147 146 145 148 146 148 150 149 145 147 1.7 10 5.4 6.1 5.7 5.6 5.9 6.3 6.9 7.6 5.5 6.2 6.1 .69 10 144 146 150 146 146 145 146 148 146 146 146 1.6 10 4.9 4.6 5.6 5.6 6.2 6.1 6.4 6.8 6.3 5.2 5.8 .71 10 105 107 106 105 108 109 106 106 108 107 107 1.3 10 100 104 104 104 107 103 106 106 106 102 104 2.1 10 Covance 6329-212 3M T-6316.1 1524 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 MEAN S.D. N Group: 2 C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 MEAN S.D. N Dose Level: 0 98 12 126 10 122 14 96 14 134 11 114 14 115 13 112 15 111 12 119 13 115 11.7 10 13 1. 5 10 Dose Level: 3 106 12 128 12 97 14 117 9 100 13 117 10 114 14 104 12 125 12 101 13 111 10.8 10 12 1. 6 10 Dosage Unit: ppm .8 7.2 .7 7.7 .7 7.5 .6 7.4 .5 7.0 .7 7.2 .6 7.3 .7 6.9 .7 7.9 .5 6.6 .6 7.3 .10 .38 10 10 Dosage Unit: ppm .7 8.1 .5 7.0 .7 8.7 .8 7.8 .7 8.6 .6 6.8 .6 7.3 .5 7.1 .8 7.2 .6 7.6 .6 7.6 .11 .67 10 10 5.0 4.5 4.3 4.3 4.4 4.3 4.6 4.1 4.2 4.3 4.4 .25 10 4.9 4.5 4.8 4.5 6.0 4.6 4.4 4.4 4.4 4.5 4.7 .49 10 2.2 3.2 3.2 3.1 2.6 2.9 2.7 2.8 3.7 2.3 2.9 .45 10 3.2 2.5 3.9 3.3 2.6 2.2 2.9 2.7 2.8 3.1 2.9 .48 10 .2 96 .0 120 .1 164 .2 247 .1 80 .2 124 .0 121 .0 102 .3 296 .1 107 .1 146 .10 70 10 10 .0 141 .2 107 .3 180 .2 110 .0 98 .0 78 .2 119 .1 179 .2 88 .4 94 .2 119 .13 36 10 10 Covance 6329-212 3M T-6316.1 1525 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 MEAN S.D. N Group: 4 C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 MEAN S.D. N Dose Level: 30 113 12 102 17 128 18 107 15 108 14 123 15 116 13 128 14 110 11 124 12 116 9.4 10 14 2.2 10 Dose Level: 100 123 15 118 15 132 14 119 13 126 14 108 15 112 13 105 15 95 16 89 14 113 13.7 10 14 1.0 10 Dosage Unit: ppm .8 7.1 .5 7.0 .8 7.2 .4 7.0 .4 7.2 .8 7.6 .8 7.4 .7 7.8 .7 7.7 .9 6.9 .7 7.3 .18 .32 10 10 Dosage Unit: ppm .5 8.0 .7 7.9 .7 7.6 .6 7.1 .6 7.5 .7 7.7 .5 7.6 .4 7.8 .7 7.3 .6 8.3 .6 7.7 .11 .35 10 10 4.6 4.7 4.7 4.5 4.9 5.0 4.7 4.5 4.5 4.5 4.7 .18 10 5.3 5.7 4.9 4.8 5.4 5.3 5.0 5.6 5.2 5.5 5.3 .30 10 2.5 2.3 2.5 2.5 2.3 2.6 2.7 3.3 3.2 2.4 2.6 .35 10 2.7 2.2 2.7 2.3 2.1 2.4 2.6 2.2 2.1 2.8 2.4 .27 10 .3 52 .1 93 .0 45 .4 65 .2 66 .0 101 .3 101 .1 176 .1 107 .1 116 .2 92 .13 38 10 10 .0 41 .0 77 .2 46 .1 31 .0 63 .2 39 .1 46 .2 91 .2 23 .1 35 .1 49 .09 21 10 10 Covance 6329-212 3M T-6316.1 1526 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 MEAN S.D. N Group: 2 C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 MEAN S.D. N Dose Level: 0 107 33 109 47 86 36 124 59 130 48 159 39 122 38 110 31 144 45 83 28 117 23.7 10 40 9.4 10 Dose Level: 3 80 44 81 42 110 57 129 39 114 45 120 45 104 42 88 34 96 42 153 85 108 22.9 10 48 14.4 10 Dosage Unit: ppm 2 11.0 1 11.3 2 11.2 0 11.1 0 11.8 7 11.1 7 11.0 7 10.6 9 11.3 1 10.3 4 3.5 10 11.1 .41 10 Dosage Unit: ppm 1 11.2 0 10.6 6 11.5 6 11.0 0 11.3 7 10.3 3 10.9 4 10.8 4 11.0 0 11.2 3 2.7 10 11.0 .35 10 6.9 6.0 6.3 6.0 8.2 7.0 7.1 6.5 6.5 4.9 6.5 .87 10 6.9 6.3 7.9 6.7 5.9 6.4 6.8 6.5 6.8 7.0 6.7 .53 10 151 150 148 148 150 152 148 151 152 148 150 1.7 10 6.7 6.3 6.8 6.1 5.8 6.7 6.7 6.4 6.3 5.9 6.4 .36 10 152 150 153 148 149 150 151 151 148 150 150 1.6 10 6.7 6.7 6.8 7.1 5.4 6.1 5.9 6.8 6.5 6.8 6.5 .52 10 112 108 106 107 107 108 108 110 108 113 109 2.3 10 108 110 107 105 108 108 107 108 108 109 108 1.3 10 Covance 6329-212 3M T-6316.1 1527 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 MEAN S.D. N Group: 4 C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 MEAN S.D. N Dose Level: 30 133 73 112 37 109 35 148 82 109 56 79 38 125 76 76 40 75 30 88 44 105 25.4 10 51 19.2 10 Dose Level: 100 87 110 94 53 79 72 73 36 85 54 110 116 104 69 68 55 57 43 76 70 83 16.2 10 68 26.5 10 Dosage Unit: ppm 0 10.9 8 10.6 0 10.4 8 10.7 0 11.0 5 11.2 10 11.1 0 11.3 0 11.2 10 10.7 4 4.5 10 10.9 .30 10 Dosage Unit: ppm 5 10.9 8 10.7 0 11.4 7 10.7 3 11.0 13 10.9 11 10.8 7 11.0 6 10.9 0 10.9 6 4.2 10 10.9 .20 10 6.3 5.9 6.3 7.4 6.2 7.0 7.9 6.8 6.9 7.2 6.8 .62 10 6.2 6.4 6.7 5.9 6.4 6.3 6.5 7.0 7.1 6.2 6.5 .37 10 151 148 148 151 150 149 151 152 151 146 150 1. 9 10 6.9 6.4 6.6 6.6 6.2 5.8 6.5 7.3 6.2 6.2 6.5 .42 10 147 146 147 146 148 149 146 148 149 151 148 1. 6 10 6.2 6.4 7.2 5.8 5.8 6.6 5.9 6.1 6.6 6.6 6.3 .45 10 112 109 111 108 109 107 110 110 109 107 109 1 10 109 110 109 108 108 113 107 107 109 112 109 2 10 Covance 6329-212 3M T-6316.1 1528 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 1 C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 MEAN S.D. N Group: 2 C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 MEAN S.D. N Dose Level: 0 108 12 107 12 110 14 115 8 124 12 88 16 119 13 105 11 81 18 122 17 108 14.0 10 13 3. 0 10 Dose Level: 3 107 13 126 14 113 13 91 11 89 13 117 12 104 11 116 13 103 11 88 16 105 13.0 10 13 1. 6 10 Dosage Unit: ppm .7 7.9 .9 8.5 .8 7.6 .6 7.5 .6 7.7 .6 7.9 .6 8.5 .9 8.9 .7 6.8 .8 9.1 .7 8.0 .12 .70 10 10 Dosage Unit: ppm .6 7.4 .7 7.6 .9 8.3 .6 7.9 .7 8.6 .4 8.8 .8 8.5 .8 8.0 .8 9.2 .4 8.1 .7 8.2 .17 .55 10 10 5.9 6.2 5.3 5.5 5.9 5.7 6.0 6.6 5.1 7.0 5.9 .58 10 4.7 5.3 6.1 5.5 5.3 6.7 6.1 5.8 6.2 5.9 5.8 .57 10 2.0 2.3 2.3 2.0 1.8 2.2 2.5 2.3 1.7 2.1 2.1 .25 10 2.7 2.3 2.2 2.4 3.3 2.1 2.4 2.2 3.0 2.2 2.5 .40 10 .2 91 .2 136 .4 86 .2 126 .5 83 .0 112 .4 144 .1 132 .4 128 .4 125 .3 116 .16 22 10 10 .3 95 .1 140 .2 100 .0 71 .1 197 .3 112 .0 111 .1 72 .0 144 .6 151 .2 119 .19 39 10 10 Covance 6329-212 3M T-6316.1 1529 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 3 C91255 C91258 C91264 C91269 C91270 C91274 SH C91289 C91294 C91297 C91305 MEAN S.D. N Group: 4 C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 MEAN S.D. N Dose Level: 30 76 16 99 12 100 13 95 15 93 18 99 12 94 13 83 15 92 11 99 13 93 7.8 10 14 2.1 10 Dose Level: 100 88 26 101 15 86 15 122 14 107 13 98 21 110 16 113 17 109 12 96 15 103 11.3 10 16 4.2 10 Dosage Unit: ppm .8 8.5 .8 9.0 .7 8.3 .8 7.8 .9 8.4 .9 8.5 .6 8.8 .8 8.1 .8 7.9 .8 8.2 .8 8.4 .09 .37 10 10 Dosage Unit: ppm .6 8.4 .8 8.0 .7 8.0 .6 7.9 .5 8.9 .6 9.1 .7 8.5 .7 8.5 .6 8.7 .6 8.9 .6 8.5 .08 .42 10 10 6.1 6.0 5.8 5.6 6.2 6.1 5.9 5.5 6.0 5.9 5.9 .22 10 6.4 5.7 5.8 5.7 6.1 5.9 5.9 5.8 5.9 6.1 5.9 .22 10 2.4 3.0 2.5 2.2 2.2 2.4 2.9 2.6 1.9 2.3 2.4 .33 10 2.0 2.3 2.2 2.2 2.8 3.2 2.6 2.7 2.8 2.8 2.6 .37 10 .3 61 .2 94 .2 102 .3 72 .1 94 .9 124 .1 92 .0 60 .4 115 .2 126 .3 94 .25 23.9 10 10 .3 65 .3 86 .0 69 .2 68 .2 79 .0 144 .0 83 .1 96 .1 104 .2 89 .1 88 .12 23.2 10 10 Covance 6329-212 3M T-6316.1 1530 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 1 C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 MEAN S.D. N Group: 2 C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 MEAN S.D. N Dose Level: 0 116 63 80 41 113 36 112 33 63 31 85 31 130 51 85 32 184 80 106 36 107 33.7 10 43 16. 5 10 Dose Level: 3 131 44 116 69 84 45 95 29 84 40 101 66 97 35 68 22 164 95 97 39 104 27.4 10 48 22. 0 10 Dosage Unit: ppm 3 11.0 1 11.5 0 10.9 0 10.2 4 11.2 4 10.8 4 11.4 0 11.5 0 10.6 2 11.5 2 1.8 10 11.1 .44 10 Dosage Unit: ppm 0 10.3 1 10.8 7 11.6 1 11.5 7 11.6 1 11.6 0 11.0 4 11.1 0 11.5 0 11.2 2 2.8 10 11.2 .43 10 5.2 5.6 5.6 4.6 6.3 5.6 6.0 5.9 6.0 7.4 5.8 .73 10 5.9 5.1 5.9 6.2 6.5 5.9 5.5 6.0 5.2 5.5 5.8 .44 10 147 148 151 148 145 147 148 148 144 151 148 2.2 10 5.7 5.6 5.6 5.7 6.7 5.1 5.1 6.1 5.0 5.6 5.6 .51 10 148 150 148 151 150 148 146 149 150 146 149 1.7 10 6.4 6.3 5.9 6.6 5.3 6.6 5.9 6.2 5.4 6.0 6.1 .45 10 107 106 111 109 106 106 102 107 101 100 106 3 10 111 110 106 109 102 107 106 111 107 109 108 2 10 Covance 6329-212 3M T-6316.1 1531 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 3 C91255 C91258 C91264 C91269 C91270 C91274 SH C91289 C91294 C91297 C91305 MEAN S.D. N Group: 4 C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 MEAN S.D. N Dose Level: 30 138 54 105 48 143 49 118 43 84 35 275 270 119 37 100 46 77 30 92 52 125 56. 9 10 66 72.0 10 Dose Level: 100 81 57 143 125 86 41 92 83 82 73 95 53 74 41 82 46 78 43 79 67 89 19.9 10 63 26.1 10 Dosage Unit: ppm 2 10 .6 1 11 .2 0 10 .8 2 10 .8 23 11 .9 0 10 .6 3 11 .4 7 11 .5 7 11 .2 5 11 .2 5 6.8 10 11 .1 .42 10 Dosage Unit: ppm 0 11.3 4 11.2 9 10.7 7 11.2 0 11.3 0 11.8 0 11.1 9 11.3 1 11.3 8 11.6 4 4.0 10 11.3 .29 10 6 .2 5 .9 5 .5 5 .7 7 .4 5 .0 7 .7 6 .4 5 .5 6 .2 6 .2 .85 ]_0 5.7 5.9 6.2 6.2 6.3 6.2 5.0 5.1 5.6 5.4 5.8 .48 10 149 151 148 145 155 145 157 150 148 150 150 3.9 10 5 .0 6 .0 5 .6 5 .2 5 .8 6 .3 6 .5 6 .8 5 .6 5 .8 5 .9 .56 10 148 146 145 148 147 146 149 143 149 147 147 1.9 10 6.0 6.2 4.9 5.6 5.9 5.4 6.0 6.0 5.6 5.3 5.7 .40 10 103 107 109 105 108 107 110 111 107 106 107 2.4 10 110 107 101 107 108 105 110 109 109 104 107 2 10 Covance 6329-212 3M T-6316.1 1532 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 1 C90712 C90716 C90722 C90724 C90726 C90728 C90735 C90742 C90743 C90747 C90750 C90758 C90765 C90778 MEAN S.D. N Dose Level: 0 293 115 212 270 87 156 121 268 102 70 108 97 132 141 114 98 260 508 114 171 84 130 187 97 182 325 138 194 152 65.0 14 189 119.3 14 Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1533 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 2 C90781 C90782 C90783 C90785 C90787 C90788 C90791 C90794 C90799 C90804 C90809 C90810 C90811 C90812 C90815 C90823 C90829 C90830 C90832 C90835 C90838 C90840 MEAN S.D. N Dose Level: 3 212 616 177 188 113 153 100 151 59 47 68 128 110 231 82 64 161 177 170 148 75 129 215 249 108 167 136 171 120 85 126 201 89 130 96 36 138 157 418 356 297 686 94 117 144 83.4 22 199 162.2 22 Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1534 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 3 C90842 C90844 C90850 C90851 C90854 C90855 C90856 C90858 C90860 C90864 C90865 C90867 C90869 C90871 C90881 C90882 C90883 C90884 C90885 C90889 C90893 C90900 MEAN S.D. N Dose Level: 30 107 270 85 41 78 85 86 147 47 83 97 109 186 343 135 113 73 68 114 157 84 163 97 146 143 85 119 160 143 129 99 54 96 160 105 109 75 38 160 187 156 56 108 91 109 33.4 22 127 73.2 22 Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1535 Appendix 5 Individual Clinical Chemistry Data Males Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 4 C90904 C90908 C90910 C90911 C90916 C90926 C90927 C90928 C90933 C90944 C90947 C90948 C90953 C90955 C90956 C90965 C90968 MEAN S.D. N Group: 6 C91047 C91054 C91056 C91060 C91065 C91070 C91071 C91072 C91073 C91076 MEAN S.D. N Dose Level: 100 56 59 60 86 206 389 16 20 67 48 53 200 164 276 65 27 58 59 88 39 61 62 82 64 145 303 71 75 105 94 34 26 50 44 81 48. 7 17 110 111 17 Dose Level: 100 114 221 95 127 60 97 113 189 191 469 96 113 279 251 103 118 113 91 37 56 120 68..7 10 173 120 10 Dosage Unit: ppm Dosage Unit: ppm 1536 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 1 C91127 C91131 C91138 C91144 C91148 C91157 C91159 C91166 C91172 C91176 C91183 C91188 C91190 MEAN S.D. N Group: 2 C91197 C91201 C91207 C91211 C91216 C91220 C91221 C91227 C91232 C91235 C91236 C91242 C91243 MEAN S.D. N Dose Level: 0 72 178 86 35 152 144 107 53 99 353 128 175 110 244 122 296 116 89 101 62 103 195 109 107 203 229 116 32.6 13 166 97. 3 13 Dose Level: 3 108 438 121 68 128 42 126 61 75 141 131 120 117 217 124 184 137 428 70 188 109 208 60 66 113 117 109 24.9 13 175 128. 4 13 Dosage Unit: ppm Dosage Unit: ppm 1537 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 3 C91252 C91253 C91254 C91256 C91264 C91278 C91279 C91286 C91292 C91294 C91295 C91301 C91303 C91307 C91310 MEAN S.D. N Dose Level : 30 99 88 89 315 105 47 101 97 86 53 95 86 86 114 74 45 129 367 85 49 166 136 110 66 79 138 104 32 93 106 100 22.8 15 116 97 15 Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1538 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 4 C91315 C91324 C91327 C91332 C91335 C91336 C91338 C91340 C91341 C91342 C91356 C91357 C91360 C91361 C91362 C91372 C91376 C91378 C91380 MEAN S.D. N Dose Level: 100 126 91 90 61 153 289 90 18 86 54 93 30 63 41 69 27 104 56 88 62 254 213 77 53 70 43 128 68 150 332 88 66 103 66 74 41 140 97 108 44.8 19 90 88.3 19 Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1539 Appendix 5 Individual Clinical Chemistry Data Females Week 105 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER CHOL MG/DL TRIG MG/DL Group: 6 C91451 C91453 C91455 C91459 C91462 C91467 C91473 C91479 C91480 C91485 C91487 C91488 MEAN S.D. N Dose Level: 100 118 160 111 1350 102 586 213 391 150 87 171 131 128 396 284 1525 150 386 96 154 179 314 142 305 154 53.4 12 482 469.7 12 Dosage Unit: ppm Covance 6329-212 3M T-6316.1 1540 Appendix 5 Individual Clinical Urinalysis Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C90717 C90718 C90737 C90756 C90769 MEAN S.D. N Group: 5 C90971 C90972 C90988 C90996 C91017 MEAN S.D. N Dose Level: 0 7.5 8.6 14.3 31.8 25.2 1.029 1.027 1.020 1.010 1.012 17.5 10.64 5 1.020 .0086 5 Dose Level: 300 22.1 27.0 46.5 a 28.6 18.2 1.007 1.009 1.005 1.012 1.017 28.5 10.88 5 1.010 .0047 5 Dosage Unit: ppm 7.0 6.5 7.5 7.0 7.0 7.0 .35 5 Dosage Unit: ppm 7.0 7.0 7.0 7.0 6.5 6.9 .22 5 a Sample was overflowing at the time of collection. Covance 6329-212 3M T-6316.1 1541 Appendix 5 Individual Clinical Urinalysis Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C90717 C90718 C90737 C90756 C90769 Group: 5 C90971 C90972 C90988 C90996 C91017 Dose Level: 0 30 30 30 10 30 - Dose Level: 300 30 30 10 30 30 - Dosage Unit: ppm ++ -+-- -- - + - - Dosage Unit: ppm ++ --- ++++ - - + - - Covance 6329-212 3M T-6316.1 1542 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C90717 C90718 C90737 C90756 C90769 Group: 5 C90971 C90972 C90988 C90996 C91017 Dose Level: 0 01 11 10 01 00 Dose Level: 300 00 00 01 01 00 Dosage Unit: ppm 12 0 110 110 100 110 Dosage Unit: ppm 110 110 110 110 110 4D 4D 3D 2D 4D 0 3D 1B1D D 3D CJ BJ BJ BJ BJ AJ BJ AJ BJ BJ Q PQ PQ Q PQ Q Q PQ PQ PQ Covance 6329-212 3M T-6316.1 1543 Appendix 5 Individual Clinical Urinalysis Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C91126 C91140 C91145 C91162 C91163 MEAN S.D. N Group: 5 C91393 C91394 C91404 C91415 C91438 MEAN S.D. N Dose Level: 0 26.0 46.2 a 20.0 5.0 2.3 1.006 1.003 1.006 1.018 1.027 19.9 17.75 5 1.012 .0102 5 Dose Level: 300 21.3 19.0 14.1 10.8 43.0 a 1.008 1.011 1.015 1.013 1.002 21.6 12.63 5 1.010 .0051 5 Dosage Unit: ppm 7.0 6.5 7.0 7.0 6.5 6.8 .27 5 Dosage Unit: ppm 7.5 6.5 6.5 6.5 6.5 6.7 .45 5 a Sample was overflowing at the time of collection. Covance 6329-212 3M T-6316.1 1544 Appendix 5 Individual Clinical Urinalysis Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C91126 C91140 C91145 C91162 C91163 Group: 5 C91393 C91394 C91404 C91415 C91438 Dose Level: 0 0010 10 10 - Dose Level: 300 00000- Dosage Unit: ppm ----------- Dosage Unit: ppm --++ - ------- - - - - Covance 6329-212 3M T-6316.1 1545 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C91126 C91140 C91145 C91162 C91163 Group: 5 C91393 C91394 C91404 C91415 C91438 Dose Level: 0 00 00 01 00 00 Dose Level: 300 00 00 00 00 01 Dosage Unit: ppm 110 110 110 110 110 Dosage Unit: ppm 110 010 010 110 110 1D 0 0 1B1D 3D 0 0 1B 0 1B AJ AJ AJ BJ CJ BJ BJ BJ BJ AJ - - Covance 6329-212 3M T-6316.1 1546 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 MEAN S.D. N Group: 2 C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 MEAN S.D. N Dose Level: 0 15.6 19.8 16.0 2.8 12.6 46.0 4.3 9.2 17.4 43.5 1.018 1.020 1.020 1.057 1.023 1.006 1.040 1.027 1.021 1.007 18.7 14.79 10 1.024 .0151 10 Dose Level: 3 47.3 42.5 9.7 13.0 31.7 10.5 14.0 21.5 46.5 14.0 1.004 1.006 1.018 1.023 1.012 1.026 1.018 1.016 1.007 1.024 25.1 15.47 10 1.015 .0079 10 Dosage Unit ppm 7.0 7.0 7.0 6.0 7.5 7.0 6.5 7.0 7.0 7.0 6.9 .39 10 Dosage Unit ppm 7.0 6.5 7.0 7.0 7.0 6.5 7.0 7.0 7.0 7.0 6.9 .21 10 Covance 6329-212 3M T-6316.1 1547 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 MEAN S.D. N Group: 4 C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 MEAN S.D. N Dose Level: 30 13.1 16.7 25.0 14.2 6.4 11.8 33.8 8.5 3.2 24.8 1.022 1.015 1.012 1.018 1.039 1.019 1.012 1.027 1.043 1.015 15.8 9.53 10 1.022 .0109 10 Dose Level: 100 11.7 15.3 18.3 47.0 9.2 4.0 46.8 26.6 13.8 6.5 1.021 1.018 1.016 1.003 1.026 1.058 1.006 1.012 1.024 1.037 19.9 15.56 10 1.022 .0160 10 Dosage Unit: ppm 6.5 7.0 7.0 7.0 7.0 7.0 6.5 6.5 6.5 7.0 6.8 .2 10 Dosage Unit: ppm 7.5 7.0 7.0 7.0 6.5 6.5 6.5 6.5 6.5 6.5 6.8 .3 10 Covance 6329-212 3M T-6316.1 1548 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 Group: 2 C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 Dose Level: 0 10 10 30 30 30 10 100 30 10 10 - Dose Level: 3 010 10 10 10 30 10 10 10 10 - Dosage Unit: ppm ++-++ +-+--- -- - -+++ + + - Dosage Unit: ppm --- ----+---+---------- - - - Covance 6329-212 3M T-6316.1 1549 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 Group: 4 C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 Dose Level: 30 10 10 10 10 30 10 10 30 30 10 - Dose Level: 100 30 10 10 030 30 010 10 30 - Dosage Unit: ppm +---+---++ - -- - - - Dosage Unit: ppm --- +-+---+-+-----+---- - - Covance 6329-212 3M T-6316.1 1550 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 Group: 2 C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 Dose Level: 0 00 00 00 00 00 00 40 01 20 00 Dose Level: 3 00 00 00 00 00 00 00 00 00 00 Dosage Unit: ppm 110 110 010 110 110 110 130 12 0 030 12 0 Dosage Unit: ppm 110 110 110 110 12 0 110 12 0 110 130 12 0 4D BJ PQ 1D BJ PQ 4D BJ PQ 4D CJ PQ 4D BJ Q 0 AJ Q 4D DK P 4D BJ PQ 4D BJ PQ 2D AJ Q 0 AJ Q 3D AJ Q 4D BJ PQ 4D BJ PQ 4D BJ Q 0 BJ Q 4D BJ PQ 4D BJ Q 3D AJ Q 4D BJ PQ Covance 6329-212 3M T-6316.1 1551 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 Group: 4 C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 Dose Level: 30 01 00 00 00 01 00 00 01 00 00 Dose Level: 100 00 00 10 00 01 00 00 00 10 00 Dosage Unit:: ppm 12 0 110 110 110 130 010 12 0 110 12 0 12 0 Dosage Unit: ppm 12 0 110 010 110 110 12 0 010 110 110 110 2B2D 2D 4D 4D 1B3D 2D 2D 1B3D 4D 0 2D 2D 1B 0 0 4D 0 0 3D 4D BJ BJ BJ BJ BK BJ BJ CJ CJ BJ BJ BJ BJ AJ BJ CJ AJ AJ BJ CJ Q Q Q Q Q Q PQ PQ PQ Q Q Q Q PQ PQ Q Q Q PQ Covance 6329-212 3M T-6316.1 1552 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 MEAN S.D. N Group: 2 C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 MEAN S.D. N Dose Level: 0 27.9 11.5 16.8 15.9 49.8 7.8 5.6 10.5 28.2 15.8 1.003 1.014 1.009 1.010 1.002 1.025 1.025 1.018 1.007 1.010 19.0 13.21 10 1.012 .0082 10 Dose Level: 3 2.4 11.4 26.5 21.8 45.6 47.0 9.0 2.5 33.5 22.3 1.031 1.013 1.008 1.007 1.002 1.002 1.020 1.026 1.006 1.006 22.2 16.26 10 1.012 .0102 10 Dosage Unit ppm 7.0 6.5 6.5 6.5 6.5 6.5 6.5 7.0 7.0 7.0 6.7 .26 10 Dosage Unit ppm 6.5 7.0 6.5 7.0 7.0 6.5 7.0 6.5 6.5 7.0 6.8 .26 10 Covance 6329-212 3M T-6316.1 1553 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 MEAN S.D. N Group: 4 C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 MEAN S.D. N Dose Level: 30 23.0 7.3 49.0 15.7 7.2 1.3 7.0 47.0 17.3 17.1 1.004 1.020 1.002 1.010 1.016 1.038 1.020 1.002 1.011 1.015 19.2 16.50 10 1.014 .0109 10 Dose Level: 100 5.6 46.0 18.6 20.7 11.8 47.8 40.0 8.2 25.5 7.8 1.027 1.002 1.013 1.007 1.018 1.002 1.006 1.022 1.009 1.018 23.2 16.13 10 1.012 .0086 10 Dosage 7.0 6.5 6.0 7.0 7.0 6.0 6.5 7.0 6.5 6.5 6.6 .39 10 Dosage 6.5 6.5 6.5 6.5 6.5 7.0 6.5 6.5 6.5 6.5 6.6 .16 10 Covance 6329-212 3M T-6316.1 1554 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 Group: 2 C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 Dose Level: 0 010 00010 10 10 00- Dose Level: 3 10 0000010 10 00- Dosage Unit: ppm -__ ---__ -__ -__ -__ -__ -__ -__ -__ Dosage Unit: ppm -__ -__ -__ -__ -__ -__ -__ -__ -__ -__ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Covance 6329-212 3M T-6316.1 1555 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 Group: 4 C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 Dose Level: 30 010 00010 10 010 0- Dose Level: 100 10 00010 0010 010 - Dosage Unit: ppm --------------------- Dosage Unit: ppm ------------------- - - Covance 6329-212 3M T-6316.1 1556 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 Group: 2 C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 Dose Level: 0 00 01 00 00 00 01 01 00 00 00 Dose Level: 3 00 00 00 00 00 00 00 00 00 00 Dosage Unit: ppm 010 12 0 110 110 12 0 110 030 010 12 0 110 Dosage Unit: ppm 010 12 0 02 0 110 010 010 010 110 110 110 1B1D 2B 0 0 0 4D 4D 4D 2D 4D 4D 2D 0 1B 1B 0 1D 0 1B 0 AJ BJ BJ AJ AJ BJ BJ BJ AJ AJ BJ BJ AJ BJ AJ AJ BJ BJ AJ AJ P P P P P P P - Covance 6329-212 3M T-6316.1 1557 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 Dose Level: 30 C91262 C91274 C91281 C91284 C91286 C91288 QS C91293 C91299 C91304 C91305 0 0 0 0 0 - 0 0 0 0 0 0 0 0 0 - 0 0 0 0 Group: 4 Dose Level: 100 C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 Dosage Unit: ppm 110 12 0 110 12 0 010 --110 010 110 010 Dosage Unit: ppm 110 02 0 010 110 110 02 0 010 110 010 110 0 4D 0 0 2D 0 0 0 0 1B 0 0 1B1D 1B1D 0 1B 4D 2B 0 AJ BJ AJ AJ BJ BJ BJ AJ BJ BJ BJ AJ BJ AJ BJ AJ AJ BJ AJ BJ P P P - P P P Covance 6329-212 3M T-6316.1 1558 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 MEAN S.D. N Group: 2 C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 MEAN S.D. N Dose Level: 0 30.5 11.5 7.2 10.7 7.4 14.7 15.4 23.7 24.9 8.7 1.012 1.021 1.036 1.026 1.033 1.021 1.020 1.016 1.017 1.037 15.5 8.17 10 1.024 .0087 10 Dose Level: 3 24.4 16.7 10.5 20.5 14.5 10.1 8.3 7.9 15.6 8.5 1.011 1.017 1.025 1.021 1.029 1.024 1.031 1.031 1.019 1.043 13.7 5.65 10 1.025 .0090 10 Dosage Unit ppm 6.5 7.0 7.0 7.0 6.5 7.0 7.0 7.5 7.5 7.0 7.0 .33 10 Dosage Unit ppm 7.5 7.5 7.0 7.5 7.5 7.0 7.0 7.0 7.5 6.5 7.2 .35 10 Covance 6329-212 3M T-6316.1 1559 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 MEAN S.D. N Group: 4 C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 MEAN S.D. N Dose Level: 30 38.5 12.0 4.0 6.7 10.0 38.5 5.0 6.0 5.8 10.1 1.011 1.031 1.052 1.032 1.022 1.009 1.042 1.043 1.034 1.028 13.7 13.33 10 1.030 .0137 10 Dose Level: 100 47.5 a 5.0 27.5 6.8 21.7 10.5 8.2 6.3 9.8 11.2 1.009 1.037 1.011 1.037 1.016 1.027 1.029 1.028 1.031 1.028 15.4 13.36 10 1.025 .0100 10 Dosag 7.5 6.5 7.0 7.0 7.0 7.0 6.5 7.5 7.0 7.0 7.0 .33 10 Dosag 7.0 7.5 7.0 7.0 7.0 6.5 6.5 7.0 7.5 7.0 7.0 .33 10 ppm ppm a Sample was overflowing at the time of collection. 1560 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 Dose Level: 0 C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 100 30 300 300 30 30 30 30 30 300 - Group: 2 Dose Level: 3 C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 10 30 30 100 100 30 30 100 30 >2000 - Dosage Unit: ppm - - ++ +-+-+---------+ +---- Dosage Unit: ppm --- - +++ - - ++ - - +++ +-+ +-+---+-- - +++ - - Covance 6329-212 3M T-6316.1 1561 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 Group: 4 C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 Dose Level: 30 10 30 100 30 30 10 100 30 30 30 - Dose Level: 100 10 30 30 100 30 30 30 30 30 30 - Dosage Unit: ppm -__ +-+__ +__ +__ -__ +__ -__ +__ +__ Dosage Unit: ppm -__ +++ _ _ -__ ++ _ _ -__ +__ +__ +__ +__ +__ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Covance 6329-212 3M T-6316.1 1562 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 Group: 2 C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 Dose Level: 0 01 00 01 00 00 00 00 00 00 00 Dose Level: 3 00 10 00 10 00 00 01 00 01 40 Dosage Unit: ppm 0 1 1G 12 0 110 12 0 110 12 0 110 110 110 02 0 Dosage Unit: ppm 110 010 12 0 110 02 0 110 12 0 12 0 110 12 0 1B 4D 4D 4D 0 4D 4D 3D 3D 2B4D 3D 3D 4D 3D 2B4D 4D 4D 4D 4D 4D BJ BK CK BK CK BK BK BJ BJ CK BJ BK BK BK BK BK BK BK BK DK PQ PQ PQ PQ PQ P P Q PQ Q PQ P PQ PQ Q PQ PQ P Covance 6329-212 3M T-6316.1 1563 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 Group: 4 C90916 C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 Dose Level: 30 00 00 00 00 00 00 41 00 01 01 Dose Level: 100 00 00 01 00 00 00 00 00 00 14 Dosage Unit:: ppm 110 110 130 12 0 010 110 12 0 010 110 110 Dosage Unit: ppm 010 110 110 110 12 0 110 12 0 010 12 0 02 0 0 1D 2B4D 4D 2D 0 1B1D 4D 3D 3D 0 4D 0 4D 4D 4D 4D 2D 4D 0 BJ BK DKO BK BK BJ DL CK CK BK BJ CK BJ CK BK BK BK BK BK BK Q Q PQT Q PQ P Q PQ Q Q Q P PQ PQ PQ PQ Q PQ PQ Covance 6329-212 3M T-6316.1 1564 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 MEAN S.D. N Group: 2 C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 MEAN S.D. N Dose Level: 0 2.2 11.2 8.8 47.1 a 2.0 11.0 16.0 11.0 49.4 a 22.1 1.044 1.018 1.018 1.003 1.042 1.017 1.016 1.023 1.005 1.008 18.1 16.96 10 1.019 .0140 10 Dose Level: 3 12.6 3.3 4.0 6.2 6.4 10.6 11.1 50.6 a 35.0 19.6 1.010 1.027 1.032 1.021 1.024 1.018 1.012 1.001 1.006 1.009 15.9 15.38 10 1.016 .0100 10 Dosage 6.5 6.5 7.0 6.5 7.0 7.0 7.0 7.0 7.5 7.0 6.9 .32 10 Dosage 6.5 6.5 6.5 7.0 7.5 7.5 6.5 6.5 7.0 7.0 6.8 .41 10 a Sample was overflowing at the time of collection. 1565 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 MEAN S.D. N Group: 4 C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 MEAN S.D. N Dose Level: 30 49.4 a .8 8.7 8.5 11.4 14.0 8.0 5.5 10.5 13.1 1.003 1.072 1.016 1.009 1.016 1.011 1.018 1.017 1.017 1.019 13.0 13.35 10 1.020 .0190 10 Dose Level: 100 8.5 12.1 6.5 7.8 5.7 27.8 5.8 23.3 14.1 20.5 1.018 1.018 1.026 1.023 1.033 1.008 1.026 1.008 1.017 1.013 13.2 8.01 10 1.019 .0081 10 Dosa 6.5 6.0 7.0 7.0 6.5 7.0 6.5 6.5 7.0 7.0 6.7 .3 10 Dosa 6.5 6.0 6.5 7.0 7.5 6.5 6.5 7.0 6.5 7.5 6.8 .4 10 ppm ppm a Sample was overflowing at the time of collection. 1566 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 Group: 2 C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 Dose Level: 0 30 10 10 10 30 10 10 10 00- Dose Level: 3 10 30 10 10 10 10 10 010 10 - Dosage Unit: ppm --------------- - +++ --+ --- Dosage Unit: ppm ----------+ ----------- - - Covance 6329-212 3M T-6316.1 1567 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 Group: 4 C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 Dose Level: 30 10 30 10 010 010 10 10 10 - Dose Level: 100 10 10 30 10 10 010 010 10 - Dosage Unit: ppm --------------------- Dosage Unit: ppm ------------------- - - Covance 6329-212 3M T-6316.1 1568 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C91127 C91128 C91133 C91137 C91144 C91153 C91154 C91158 C91161 C91183 Group: 2 C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 C91243 C91244 Dose Level: 0 00 00 01 00 00 00 00 41 00 00 Dose Level: 3 00 01 00 00 10 00 00 00 00 00 Dosage Unit: ppm 030 130 110 12 0 110 12 0 110 12 0 030 110 Dosage Unit: ppm 110 12 0 12 0 12 0 010 110 110 010 12 0 110 1D 0 2D 0 2B4D 4D 2D 4D 3B 1D 0 0 4D 4D 4D 4D 0 0 0 0 CJ BJ BJ AJ CK BJ BJ BK BK BJ BJ CJ BK BK BK BJ BJ AJ BJ BJ P P P P P P - P P P P P P - Covance 6329-212 3M T-6316.1 1569 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C91252 C91254 C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 Group: 4 C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 Dose Level: 30 00 00 00 00 01 00 00 01 00 01 Dose Level: 100 01 00 01 00 01 00 00 00 00 00 Dosage Unit: ppm 010 110 110 010 110 010 02 0 110 110 110 Dosage Unit: ppm 110 12 0 130 110 110 110 12 0 010 110 130 0 3D 4D 1B1D 1D 1D 2B2D 0 0 2D 0 0 0 4D 4D 0 0 1D 0 2B3D AJ DK BJ BJ BJ BJ BK BJ BJ BK BJ BJ CK BJ BK BJ BJ BJ BJ BJ P P P P P P P P Covance 6329-212 3M T-6316.1 1570 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 MEAN S.D. N Group: 2 C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 MEAN S.D. N Dose Level: 0 7.5 48.5 a 11.2 17.7 49.0 a 16.3 11.0 7.6 15.0 9.3 1.027 1.010 1.030 1.020 1.011 1.020 1.026 1.035 1.026 1.038 19.3 15.90 10 1.024 .0092 10 Dose Level: 3 11.5 13.0 7.8 15.5 11.1 16.2 4.5 8.2 25.3 23.7 1.027 1.026 1.037 1.024 1.027 1.020 1.050 1.040 1.018 1.017 13.7 6.72 10 1.029 .0106 10 Dosa< 7.0 6.5 7.0 6.5 7.0 6.5 7.0 6.5 7.0 7.0 6.8 .2 10 Dosa 6.5 7.0 7.0 7.0 7.0 7.5 7.0 6.5 7.0 7.5 7.0 .3 10 ppm ppm a Sample was overflowing at the time of collection. 1571 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 MEAN S.D. N Group: 4 C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 MEAN S.D. N Dose Level: 30 19.3 4.3 17.0 13.3 5.6 21.7 25.0 6.8 12.0 22.2 1.020 1.038 1.026 1.030 1.037 1.020 1.018 1.032 1.028 1.016 14.7 7.46 10 1.026 .0078 10 Dose Level: 100 9.0 14.5 9.5 9.0 15.7 7.0 9.2 48.0 a 24.5 27.0 1.034 1.025 1.026 1.033 1.022 1.032 1.030 1.006 1.014 1.004 17.3 12.78 10 1.023 .0110 10 Dosage 7.0 6.5 6.5 6.5 6.5 7.0 7.5 7.0 6.5 6.5 6.8 .35 10 Dosage 7.0 7.0 6.5 7.0 7.5 6.5 7.0 6.5 6.5 7.0 6.8 .34 10 ppm ppm a Sample was overflowing at the time of collection. 1572 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 Dose Level: 0 C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 >2000 100 >2000 300 100 100 30 300 300 CO - Group: 2 Dose Level: 3 C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 100 10 >2000 100 30 100 300 100 30 30 - - Dosage Unit: ppm - - ++ ----------------- - +++ Dosage Unit: ppm --------- - ++ - - +++ ----- - +++ --- - - Covance 6329-212 3M T-6316.1 1573 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 Group: 4 C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 Dose Level: 30 10 30 CO 100 30 30 10 300 30 100 - Dose Level: 100 30 30 30 30 100 30 30 030 10 - Dosage Unit: ppm --+ --- - +++ - - +++ ----------+ --- Dosage Unit: ppm --------------------- - - Covance 6329-212 3M T-6316.1 1574 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C90712 C90723 C90727 C90730 C90755 C90758 C90759 C90764 C90766 C90774 Group: 2 C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 Dose Level: 0 11 00 00 01 00 01 00 00 01 40 Dose Level: 3 00 00 00 11 31 40 00 01 11 00 Dosage Unit: ppm 12 0 110 110 1 1 1G 130 1 1 1H 110 12 0 0 2 1H 000 Dosage Unit: ppm 0 3 1G 110 110 110 110 110 12 0 110 110 110 2D 1B 1B1D 0 0 1B 1D 1B 2B 1D 4D 2D 4D 3D 2D 4D 4D 0 1B3D 4D CJ BJ CJ BJ BJ BK CK CK CK GL CJ CJ CJ CK CJ CK DK CK CK BJ Q PQ P PQ PQ PQ PQ - PQ PQ PQ P PQ P Q PQ PQ Q Covance 6329-212 3M T-6316.1 1575 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 Group: 4 C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 C90946 C90956 Dose Level: 30 10 20 40 40 01 11 00 00 02 01 Dose Level: 100 00 00 00 00 00 00 00 11 00 00 Dosage Unit:: ppm 12 0 110 010 100 12 0 110 010 12 0 130 010 Dosage Unit: ppm 110 110 010 02 0 130 010 02 0 110 110 110 3D 0 0 0 2B2D 1B1D 1B1D 4D 0 1B 4D 4D 1D 3D 2B4D 0 4D 2B 0 3D BJ CK GL CK CJ CK CJ BJ CJ CK CJ CJ CJ CK DK CJ CK AJ BK BJ PQ PQ Q P PQ PQ PQ Q PQ P Q PQ P Q P Q Q Q Covance 6329-212 3M T-6316.1 1576 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 1 C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 MEAN S.D. N Group: 2 C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 MEAN S.D. N Dose Level: 0 22.5 12.0 7.4 30.0 16.0 6.5 8.0 5.4 11.8 11.4 1.006 1.013 1.018 1.008 1.020 1.028 1.022 1.028 1.017 1.015 13.1 7.81 10 1.018 .0074 10 Dose Level: 3 49.0 a 2.5 10.5 22.2 30.6 49.0 a 11.0 5.5 8.8 4.2 1.003 1.035 1.020 1.007 1.011 1.005 1.013 1.032 1.019 1.034 19.3 17.82 10 1.018 .0122 10 Dosage 6.0 6.5 6.0 6.5 6.5 6.5 7.0 6.5 6.5 6.5 6.4 .28 10 Dosage 6.5 6.5 6.5 6.5 6.0 6.5 6.5 6.5 6.5 6.5 6.4 .16 10 a Sample was overflowing at the time of collection. 1577 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 3 C91255 C91258 C91264 C91269 C91270 C91274 C91289 C91294 C91297 C91305 MEAN S.D. N Group: 4 C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 MEAN S.D. N Dose Level: 30 16.2 9.5 49.0 a 13.0 7.5 12.5 5.0 9.0 7.5 7.6 1.016 1.017 1.004 1.021 1.033 1.011 1.030 1.018 1.021 1.037 13.7 12.84 10 1.021 .0101 10 Dose Level: 100 18.7 34.6 6.7 36.5 7.2 3.6 7.5 3.1 39.0 12.7 1.011 1.008 1.029 1.009 1.026 1.037 1.024 1.043 1.004 1.022 17.0 14.38 10 1.021 .0131 10 Dosag 6.0 6.5 6.0 6.5 6.0 6.5 6.5 6.5 6.5 6.5 6.4 .24 10 Dosag 6.5 6.5 6.5 6.5 6.0 6.5 6.5 6.5 6.5 6.5 6.4 .16 10 a Sample was overflowing at the time of collection. 1578 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 1 C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 Group: 2 C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 Dose Level: 0 0010 10 30 100 010 30 0- Dose Level: 3 10 30 00100 010 30 10 10 - Dosage Unit: ppm ----------+ - - +++ ------- --- Dosage Unit: ppm --+ ------------------+ - - Covance 6329-212 3M T-6316.1 1579 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 3 C91255 C91258 C91264 C91269 C91270 C91274 C91289 C91294 C91297 C91305 Group: 4 C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 Dose Level: 30 010 010 00010 10 0 Dose Level: 100 0030 010 100 10 10 10 0- Dosage Unit: ppm --------- - - +++ --------- Dosage Unit: ppm --- +++ -------- - - - - Covance 6329-212 3M T-6316.1 1580 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 1 C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 Group: 2 C91194 C91197 C91199 C91200 C91206 C91227 C91229 C91231 C91244 C91250 Dose Level: 0 00 00 00 01 01 42 00 10 01 01 Dose Level: 3 00 00 00 00 00 00 00 00 00 20 Dosage Unit:: ppm 110 130 130 110 110 100 110 110 110 110 Dosage Unit: ppm 130 010 02 0 110 1 1 1G 110 110 110 110 110 1B1D 0 0 0 1B 0 1D 0 0 1B 0 1D 1D 1B 0 0 0 1B 0 0 AJ BJ BJ BJ CK DL CK CJ BJ BJ AJ CJ CJ AJ BK AJ BJ CJ BJ CJ P P P P P P P P P T P - Covance 6329-212 3M T-6316.1 1581 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 3 C91255 C91258 C91264 C91269 C91270 C91274 C91289 C91294 C91297 C91305 Group: 4 C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 Dose Level: 30 00 00 00 00 40 00 01 00 00 01 Dose Level: 100 00 00 00 00 00 00 00 00 00 00 Dosage Unit:: ppm 110 110 12 0 110 010 110 110 110 110 110 Dosage Unit: ppm 130 110 12 0 110 1 1 1G 010 010 110 1 0 1G 02 0 0 0 1B1D 0 0 0 3B 2D 1B1D 0 0 0 2B 0 0 0 0 3D 0 2B BJ BJ AJ BJ GL BJ CK BJ CK CK BJ BJ CK AJ CJ CJ CJ CJ AJ CJ P P P P P P P P P P - Covance 6329-212 3M T-6316.1 1582 Appendix 5 Individual Urine Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C90717 C90718 C90737 C90756 C90769 MEAN S.D. N Group: 5 C90971 C90972 C90988 a C90996 C91017 MEAN S.D. N Dose Level: 0 44 160 36 131 24 104 19 140 10 66 27 13.5 5 120 36. 4 5 Dose Level: 300 14 28 23 38 6 18 14 56 43 63 20 14.2 5 41 18. 8 5 Dosage Unit: ppm .33 1.20 .31 1.13 .34 1.49 .60 4.45 .25 1.66 .37 .135 5 1.99 1.394 5 Dosage Unit: ppm .31 .62 .62 1.03 -- .40 1.60 .78 1.15 .53 .213 4 1.10 .403 4 a Unable to calculate excretion due to an inaccurate urine volume. Covance 6329-212 3M T-6316.1 1583 Appendix 5 Individual Urine Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C91126 C91140 a C91145 C91162 C91163 MEAN S.D. N Group: 5 C91393 C91394 C91404 C91415 C91438 a MEAN S.D. N Dose Level: 0 9 23 5 13 10 30 21 70 33 128 16 11.4 5 53 47. 3 5 Dose Level: 300 23 37 26 40 31 62 11 56 8 16 20 9.9 5 42 18. 0 5 Dosage Unit: ppm .23 .60 -- .20 .60 .10 .35 .08 .29 .15 .074 4 .46 .164 4 Dosage Unit: ppm .49 .79 .49 .76 .44 .87 .12 .60 -- .38 .178 4 .76 .113 4 a Unable to calculate excretion due to an inaccurate urine volume. Covance 6329-212 3M T-6316.1 1584 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C90716 C90731 C90738 C90745 C90746 C90748 C90760 C90766 C90768 C90780 MEAN S.D. N Group: 2 C90791 C90797 C90802 C90807 C90816 C90818 C90820 C90831 C90836 C90840 MEAN S.D. N Dose Level: 0 26 97 23 132 27 100 55 358 47 153 4 26 25 333 39 189 28 125 7 41 28 15.9 10 155 111.3 10 Dose Level: 3 8 20 3 10 28 91 53 142 24 50 22 145 22 100 19 99 7 30 32 134 22 14.5 10 82 51.3 10 Dosage Unit: ppm .41 1.51 .46 2.61 .43 1.60 .15 1.00 .59 1.93 .18 1.20 .11 1.43 .36 1.74 .49 2.18 .30 1.78 .35 .159 10 1.70 .469 10 Dosage Unit: ppm .38 .95 .13 .42 .27 .88 .69 1.85 .76 1.58 .23 1.52 .31 1.40 .41 2.13 .33 1.40 .45 1.88 .40 .197 10 1.40 .521 10 Covance 6329-212 3M T-6316.1 1585 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C90843 C90851 C90852 C90856 C90863 C90877 C90880 C90888 C90892 C90897 MEAN S.D. N Group: 4 C90905 C90907 C90917 C90921 C90934 C90947 C90953 C90957 C90960 C90968 MEAN S.D. N Dose Level: 30 11 107 21 65 11 68 29 89 64 324 16 98 10 50 33 156 82 238 20 78 30 24.4 10 127 88.4 10 Dose Level: 100 22 97 22 100 17 86 4 16 26 137 100 401 9 20 13 50 21 134 52 236 29 28.2 10 128 115.5 10 Dosage Unit: ppm .14 1.40 .35 1.09 .28 1.70 .41 1.26 .41 2.07 .19 1.16 .34 1.69 .28 1.33 .26 .76 .50 1.93 .32 .108 10 1.44 .405 10 Dosage Unit: ppm .26 1.13 .34 1.53 .31 1.57 .19 .75 .24 1.26 .40 1.60 .42 .94 .35 1.33 .29 1.85 .34 1.53 .31 .071 10 1.35 .335 10 Covance 6329-212 3M T-6316.1 1586 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C91122 C91129 C91148 C91155 C91160 C91174 C91176 C91178 C91181 C91182 MEAN S.D. N Group: 2 C91192 C91196 C91204 C91215 C91221 C91223 C91228 C91233 C91241 C91248 MEAN S.D. N Dose Level: 0 10 15 15 60 13 32 13 47 3 10 33 163 41 106 31 95 12 28 17 54 19 12.0 10 61 47. 7 10 Dose Level: 3 56 210 23 59 16 35 12 31 12 9 39 30 101 38 187 5 29 11 29 21 16.5 10 70 73. 0 10 Dosage Unit: ppm .28 .42 .17 .69 .22 .54 .21 .75 .15 .50 .26 1.27 .23 .59 .33 1.00 .34 .79 .27 .85 .25 .062 10 .74 .256 10 Dosage Unit: ppm .13 .50 .26 .67 .42 .93 .26 .68 .55 .41 .14 .42 .27 .91 .10 .47 .17 .97 .25 .65 .26 .139 10 .66 .214 10 Covance 6329-212 3M T-6316.1 1587 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C91262 C91274 C91281 C91284 C91286 C91288 C91293 C91299 C91304 C91305 MEAN S.D. N Group: 4 C91326 C91327 C91329 C91332 C91337 C91348 C91355 C91370 C91374 C91379 MEAN S.D. N Dose Level: 30 11 21 21 74 3 10 17 51 26 85 24 275 35 87 6 11 8 49 31 70 18 11.0 10 73 76. 5 10 Dose Level: 100 58 165 87 23 45 18 22 25 93 99 6 22 29 110 12 34 14 59 20 15.4 10 57 51. 3 10 Dosage Unit: ppm .25 .48 .15 .54 .15 .49 .27 .80 .19 .61 .03 .36 .24 .61 .28 .52 .14 .85 .53 1.20 .22 .132 10 .65 .244 10 Dosage Unit: ppm .32 .92 .37 .32 .43 .84 .37 .46 .30 1.10 .43 .43 .24 .88 .24 .90 .31 .87 .11 .46 .31 .098 10 .72 .271 10 Covance 6329-212 3M T-6316.1 1588 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C90721 C90735 C90736 C90738 C90739 C90747 C90751 C90752 C90755 C90766 MEAN S.D. N Group: 2 C90795 C90799 C90802 C90803 C90805 C90806 C90824 C90825 C90839 C90840 MEAN S.D. N Dose Level: 0 10 46 21 79 57 179 14 114 28 141 15 70 28 78 15 68 38 72 49 177 28 15.9 10 102 47. 8 10 Dose Level: 3 20 56 19 82 43 86 41 70 55 129 25 101 35 147 33 141 40 70 29 138 34 11.2 10 102 34. 0 10 Dosage Unit: ppi .30 1.40 .24 .91 .41 1.29 .15 1.22 .21 1.04 .22 1.03 .43 1.20 .36 1.61 .95 1.79 .43 1.54 .37 .227 10 1.30 .281 10 Dosage Unit: pp .49 1.37 .32 1.37 .45 .90 .84 1.44 .80 1.87 .25 1.02 .29 1.22 .26 1.11 .62 1.09 .25 1.17 .46 .227 10 1.26 .274 10 Covance 6329-212 3M T-6316.1 1589 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C90847 C90855 C90858 C90859 C90864 C90865 C90871 C90872 C90882 C90886 MEAN S.D. N Group: 4 C90916 a C90931 C90937 C90940 C90944 C90945 C90954 C90956 C90957 C90963 MEAN S.D. N Dose Level: 30 23 35 34 133 64 252 51 124 33 76 12 32 41 226 73 211 67 141 61 118 46 20.5 10 135 76.2 10 Dose Level: 100 5 13 67 189 18 61 45 164 21 81 39 111 28 117 61 109 86 192 57 104 43 25.3 10 114 56.2 10 Dosage Unit: ppm .89 1.35 .41 1.60 .26 1.01 .34 .83 .33 .76 .46 1.23 .20 1.13 .44 1.27 .39 .82 .62 1.19 .43 .197 10 1.12 .266 10 Dosage Unit: ppm -.34 .94 .50 1.68 .31 1.12 .46 1.76 .41 1.17 .23 .96 .38 .69 .84 1.88 .64 1.16 .46 .186 9 1.26 .413 9 a Unable to calculate due to inaccurate urine volume. 1590 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C91127 C91128 C91133 C91137 a C91144 C91153 C91154 C91158 C91161 a C91183 MEAN S.D. N Group: 2 C91196 C91207 C91210 C91211 C91214 C91221 C91224 C91235 a C91243 C91244 MEAN S.D. N Dose Level: 0 46 198 22 101 34 78 4 10 19 148 12 88 19 60 52 108 11 22 9 35 23 16.1 10 85 58. 0 10 Level: 3 6 35 29 121 51 139 52 80 62 104 50 69 14 44 37 9 26 10 40 29 22.9 10 66 43. 7 10 Dosage Unit: ppm .10 .44 .25 1.13 .30 .69 -- .04 .30 .13 .97 .30 .96 .57 1.19 -- .20 .77 .24 .164 8 .81 .318 8 Dosage Unit: ppm .08 .44 .10 .40 .20 .56 .32 .50 .40 .67 .53 .73 .16 .49 -- .32 .91 .20 .78 .26 .148 9 .61 .173 9 a Unable to calculate due to inaccurate urine volume 1591 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C91252 a C91254 QS C91264 C91267 C91283 C91286 C91292 C91295 C91301 C91303 MEAN S.D. N Group: 4 C91312 C91323 C91328 C91339 C91340 C91351 C91354 C91357 C91369 C91371 MEAN S.D. N Dose Level: 30 4 14 13 65 13 31 31 54 14 40 27 70 20 54 30 65 36 76 21 10.7 9 52 20. 2 9 Dose Level: 100 16 71 17 81 15 88 37 98 77 166 11 36 44 89 11 32 23 67 16 49 27 20.8 10 78 38. 4 10 Dosage Unit: ppm .11 .57 .11 .26 .35 .62 .20 .56 .22 .56 .11 .30 .32 .68 .47 1.00 .24 .133 8 .57 .229 8 Dosage Unit: ppm .14 .60 .21 .98 .10 .57 .29 .76 .44 .95 .31 1.00 .26 .52 .26 .75 .32 .94 .33 1.00 .27 .098 10 .81 .191 10 a Unable to calculate due to inaccurate urine volume. 1592 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C90712 C90723 a C90727 C90730 C90755 a C90758 C90759 C90764 C90766 C90774 MEAN S.D. N Group: 2 C90781 C90792 C90793 C90798 C90799 C90800 C90812 C90822 C90829 C90832 MEAN S.D. N Dose Level: 0 28 145 9 22 25 137 16 89 16 25 14 94 41 126 29 178 21 141 30 147 23 9.5 10 110 52. 5 10 Level: 3 25 133 26 141 27 174 20 117 27 130 29 85 40 270 37 205 19 83 18 76 27 7.2 10 141 60. 8 10 Dosage Unit: ppm .21 1.09 -- .28 1.53 .28 1.58 -- .23 1.53 .45 1.39 .22 1.35 .32 2.12 .28 1.37 .28 .077 8 1.50 .296 8 Dosage Unit: ppm .29 1.53 .34 1.83 .21 1.36 .31 1.81 .30 1.44 .47 1.38 .18 1.22 .30 1.68 .48 2.10 .43 1.80 .33 .102 10 1.62 .273 10 a Sample was overflowing at the time of collection. 1593 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C90841 C90850 C90854 C90855 C90856 C90859 C90873 C90879 C90881 C90896 MEAN S.D. N Group: 4 C90908 C90914 C90923 C90926 C90927 C90934 C90936 C90944 a C90946 C90956 MEAN S.D. N Dose Level: 30 27 79 45 259 17 90 48 134 23 215 32 110 27 96 29 166 19 138 14 72 28 11..2 10 136 61. 5 10 Dose Level: 100 48 176 32 136 19 94 30 195 39 103 35 163 39 162 9 29 14 48 20 54 28 12..5 10 116 58. 9 10 Dosage Unit: ppi .52 1.52 .19 1.11 .29 1.53 .64 1.78 .13 1.20 .69 2.39 .68 2.40 .20 1.13 .23 1.66 .31 1.60 .39 .221 10 1.63 .462 10 Dosage Unit: pp .43 1.58 .46 1.97 .18 .89 .27 1.76 .61 1.62 .24 1.14 .36 1.49 -- .34 1.18 .54 1.46 .38 .142 9 1.45 .335 9 a Sample was overflowing at the time of collection. 1594 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 1 C91123 C91125 C91131 C91132 C91153 C91156 C91158 C91178 C91180 C91189 MEAN S.D. N Group: C91194 C91197 C91199 C91200 C91206 C91227 a C91229 C91231 C91244 C91250 MEAN S.D. N Dose Level: 0 14 34 12 56 12 86 9 33 43 93 41 133 43 128 26 154 57 78 32 84 29 16.8 10 88 40. 9 10 Level: 3 5 10 76 205 35 100 15 32 10 47 9 23 12 64 56 162 27 102 41 176 29 23.4 10 92 68. 9 10 Dosage Unit: ppm .32 .76 .14 .67 .09 .64 .27 .99 .69 1.49 .27 .86 .34 1.02 .14 .83 .67 .92 .36 .96 .33 .206 10 .91 .240 10 Dosage Unit: pp -- .19 .51 .37 1.05 .33 .71 .31 1.44 -- .13 .70 .31 .89 .24 .90 .17 .74 .26 .086 8 .87 .283 8 a Sample was overflowing at the time of collection 1595 Covance 6329-212 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 3 C91255 C91258 C91264 a C91269 C91270 C91274 C91289 C91294 C91297 C91305 MEAN S.D. N Group: 4 C91314 C91319 C91326 C91334 C91346 C91356 C91359 C91363 C91372 C91373 MEAN S.D. N Dose Level: 30 25 70 11 76 6 16 27 106 26 150 16 49 37 136 20 80 23 93 50 232 24 12.6 10 101 60.4 10 Dose Level: 100 13 49 12 38 34 167 13 42 13 128 71 213 29 105 88 263 8 13 26 114 31 27.4 10 113 82.0 10 Dosage Unit: ppm .40 1.13 .10 .72 -- .35 1.38 .20 1.12 .20 .61 .18 .68 .18 .72 .17 .70 .38 1.76 .24 .107 9 .98 .395 9 Dosage Unit: ppm .24 .92 .42 1.31 .23 1.12 .47 1.53 .09 .92 .26 .77 .22 .79 .27 .82 .31 .51 .33 1.45 .28 .107 10 1.01 .329 10 a Sample was overflowing at the time of collection 1596 Covance 6329-212 3M T-6316.1 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-212 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e V o f X II Page 1597 o f 4873 Covance 6329-212 3M T-6316.1 VOLUME V OF XII CONTENTS Page A P P E N D I X 6 .......................................................................................................................................................................................1 5 9 9 I n d i v i d u a l A n a t o m i c P a t h o l o g y D a t a .....................................................................................................................1 6 0 0 1598 APPENDIX 6 In d iv id u a l A n a to m ic P a th o lo g y D a ta Covance 6329-212 3M T-6316.1 1599 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1 ANIMAL NUMBER: C90711 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 402.5 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 8:11 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 7 MM, DARK RED, GRAY, TAN AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE BROWN ERODED AREA, 2 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : -MINERALIZATION, CORNEAL,-MINIMAL, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL, FOCAL 1600 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 2 ANIMAL NUMBER: C90711 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 402.5 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 8:11 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE CALCULUS, 10 X 6 X 6 MM, WHITE AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN URINARY BLADDER (UB) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1601 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 3 ANIMAL NUMBER: C90711 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 402.5 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 8:11 PROSECTOR: LYNN HORNER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1602 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 4 ANIMAL NUMBER: C90712 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 397.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN -CYST(S); BILATERAL, CORTICAL SURFACE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER -LARGE; BILATERAL: EACH IS UP TO 28 X 20 X 13 MM PANCREAS (PA) : -LARGE VESSELS; DIFFUSE, UP TO 3 MM IN DIAMETER (CAP 23) ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); RIGHT: SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : -HYPERPLASIA, CORNEAL EPITHELIUM,SLIGHT, FOCAL -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, FOCAL -MINERALIZATION, CORNEAL,-MINIMAL, FOCAL KIDNEY (KD) : -CYST,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE -THROMBUS,-MODERATELY SEVERE, FOCAL PANCREAS (PA) : -HEMORRHAGE,-MODERATE, FOCAL -INFLAMMATION, VASCULAR,-MODERATELY SEVERE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -HYPERPLASIA, CORTICAL,-SLIGHT, FOCAL -MINERALIZATION,-MINIMAL, FOCAL -THROMBUS,-MODERATELY SEVERE, FOCAL 1603 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 5 ANIMAL NUMBER: C90712 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 397.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: RIGHT IS 20 X 11 X 10 MM, LEFT IS 18 X 11 X 10 MM; BOTH APPEAR SOFT PARATHYROID (PT) : -LARGE; BILATERAL: 2 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 5 X 1 MM EPIDIDYMIDES (EP) : -SMALL; BILATERAL: EACH IS 40 X 10 X 4 MM ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : -HYPERPLASIA,-MODERATE, FOCAL STOMACH, GL (ST) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 1604 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 6 ANIMAL NUMBER: C90712 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 397.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY AORTA (AO) : -LARGE; DIFFUSE: UP TO 4 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -MALOCCLUSION; BILATERAL: LOWER INCISORS -ANIMAL THIN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE AORTA (AO) : >UNREMARKABLE 1605 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 7 ANIMAL NUMBER: C90712 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 397.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 1606 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 8 ANIMAL NUMBER: C90713 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 533.2 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 8:11 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; PALE; EYE-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -WHITE INTRAOCULAR MATERIAL BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALONG MARGIN OF RIGHT CAUDAL LOBE: SINGLE TAN, SLIGHTLY RAISED AREA, 1 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : ^UNREMARKABLE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT 1607 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 9 ANIMAL NUMBER: C90713 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 533.2 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 8:11 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 10 X 6 MM, DARK RED, RED, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, DARK BROWN MATERIAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1608 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 10 ANIMAL NUMBER: C90713 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 533.2 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 8:11 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) MANDIB (SG), 1609 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 11 ANIMAL NUMBER: C90714 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0321 .0269 2.2920 2.2054 2.2469 17.9422 2.1290 .8259 1.5323 1.5470 .0108 .0136 .0050 % .0042 % .3586 % .3450 % .3515 % 2.8070 % .3331 % .1292 % .2397 % .2420 % .0017 % .0021 % OBSERVATIONS NECROPSY .0140 .0117 1.0000 .9622 .9803 7.8282 .9289 .3603 .6685 .6750 .0047 .0059 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1610 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 12 ANIMAL NUMBER: C90714 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 1611 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 13 ANIMAL NUMBER: C90714 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1612 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 14 ANIMAL NUMBER: C90715 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 617.9 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL 1613 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 15 ANIMAL NUMBER: C90715 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 617.9 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 10 MM, DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST, BROWN MATERIAL -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER AND LOWER INCISORS PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1614 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 16 ANIMAL NUMBER: C90715 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 617.9 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 14:15 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1615 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 17 ANIMAL NUMBER: C90716 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); DORSAL; WEEK 105; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -VACUOLATION,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 1616 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 18 ANIMAL NUMBER: C90716 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -RAISED AREA(S); DORSAL LUMBAR REGION: SINGLE FIRM, RED AREA, 17 X 15 X 12 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD, HAIRCOAT: DRY RED MATERIAL -BONE MARROW SMEAR TAKEN -ANIMAL OBESE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-FIBROMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1617 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 19 ANIMAL NUMBER: C90716 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 896.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1618 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 20 ANIMAL NUMBER: C90717 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:59 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0353 .0295 2.1647 1.6555 1.5543 12.1561 1.4254 .7066 1.6716 1.7505 .0146 .0107 .0076 % .0064 % .4683 % .3582 % .3363 % 2.6301 % .3084 % .1529 % .3617 % .3787 % .0032 % .0023 % OBSERVATIONS NECROPSY .0163 .0136 1.0000 .7648 .7180 5.6156 .6585 .3264 .7722 .8087 .0067 .0049 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1619 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 21 ANIMAL NUMBER: C90717 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 462.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:59 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), DUODENUM (DU) 1620 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 22 ANIMAL NUMBER: C90718 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 449.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:41 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0344 .0306 2.1686 1.3251 1.3182 10.8628 1.4621 .8431 1.4436 1.4531 .0142 .0169 .0076 % .0068 % .4822 % .2947 % .2931 % 2.4156 % .3251 % .1875 % .3210 % .3231 % .0032 % .0038 % OBSERVATIONS NECROPSY .0159 .0141 1.0000 .6110 .6079 5.0091 .6742 .3888 .6657 .6701 .0065 .0078 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1621 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 23 ANIMAL NUMBER: C90718 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 449.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:41 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), THYMUS (TH), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), EPIDIDYMIDES (EP) 1622 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 24 ANIMAL NUMBER: C90719 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 307.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:19 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 8.9709 ------------------------------------- ------------------------------- 2.9193 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1623 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 25 ANIMAL NUMBER: C90719 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 307.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:19 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1624 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 26 ANIMAL NUMBER: C90720 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/05/99 9:58 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, TAN, RED, SOFT, AND LOBULATED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MODERATE, MULTI FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 1625 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 27 ANIMAL NUMBER: C90720 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/05/99 9:58 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1626 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 28 ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC; WEEK 42; 30 X 30MM; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES -MOTTLED; ALL LOBES: DARK RED, BROWN AND TAN SPLEEN (SP) : -LARGE; 70 X 12 X 10 MM ADRENAL, CORTEX (AC) : -MASS(ES); LEFT, INVOLVING ENTIRE GLAND: SINGLE MASS, 20 MM IN DIAMETER; DARK RED, GRAY, TAN AND SOFT; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (SEE RENAL) HISTOPATHOLOGY LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SPLEEN (SP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1627 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 29 ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MESENTERIC (MS) : -LARGE; UP TO 25 MM IN DIAMETER -MOTTLED; DARK RED, GRAY AND TAN THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE GLAND: SINGLE MASS, 20 X 15 X 10 MM, DARK RED TAN AND FIRM; SAME ON CUT SURFACE; REGIONAL LN (SEE MEDIASTINAL) MUSCLE, SKELETAL (SM) : -MASS(ES); EXTENDING FROM LEFT MANDIBLE TO LEFT DORSAL ASPECT OF SKULL CAP: SINGLE MASS, 20 X 15 X 10 MM; TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MANDIBULAR) LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MARROW, FEMUR (FM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MARROW, STERNUM (SE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MUSCLE, SKELETAL (SM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1628 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 30 ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -LARGE; BILATERAL, INCLUDING DEEP CERVICAL LN'S: UP TO 12 X 10 X 8 MM; COLLECTED LN, MEDIASTINAL (ML) : -LARGE; UP TO 6 MM IN DIAMETER -DIFFUSELY RED; DARK RED (CAP 22) LN, ANT MES/PANC (AP) : -LARGE; UP TO 8 MM IN DIAMETER (CAP 23) -MOTTLED; DARK RED AND TAN LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 25 X 15 X 10 MM (CAP 26) SALIV GL, MANDIB (SG) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT LN, MANDIBULAR (MN) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, MEDIASTINAL (ML) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT MESENTERY (MY) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL LN, ANT MES/PANC (AP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, LUMBAR/ILIAC (LM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1629 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 31 ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK RED AND TAN LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 12 X 10 X 10 MM (CAP 25) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW -FLUID-THORACIC CAVITY; FILLED WITH A DARK RED FLUID LN, RENAL (RL) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1630 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 32 ANIMAL NUMBER: C90721 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/98 STUDY DAY OF DEATH: 305 STUDY WEEK OF DEATH: 44 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/98 12:55 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), TESTIS (TE), BONE, FEMUR (FE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 1631 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 33 ANIMAL NUMBER: C90722 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:29 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PANCREAS (PA) : -LARGE VESSELS; VESSELS NEAR DUODENUM: UP TO 2 MM IN DIAMETER (CAP 22) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 4 X 3 X 2 MM) PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); MID LEFT ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER HISTOPATHOLOGY LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, VASCULAR,-MODERATELY SEVERE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL -THROMBUS,-SLIGHT, FOCAL HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 1632 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 34 ANIMAL NUMBER: C90722 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:29 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -EDEMA,-SLIGHT, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1633 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 35 ANIMAL NUMBER: C90722 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 859.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:29 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1634 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 36 ANIMAL NUMBER: C90723 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 781.3 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:50 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; PROTRUDING PENIS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) PENIS (PE) : -PROTRUDING; ENTIRE LENGTH: DRY, RED, AND BROWN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PENIS (PE) : -EDEMA,-SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC ACTIVE,-SLIGHT, MULTI-FOCAL -PROLAPSE,-PRESENT 1635 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 37 ANIMAL NUMBER: C90723 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 781.3 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:50 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1636 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 38 ANIMAL NUMBER: C90724 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:41 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL 1637 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 39 ANIMAL NUMBER: C90724 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:41 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 7 X 5 MM, RED, DARK RED, TAN, GRAY AND SOFT LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 23) LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 22) FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE DARK BROWN AND MOIST ABRADED AREA, 5 MM IN DIAMETER; COLLECTED DIAPHRAGM (DP) : -MASS(ES); ATTACHED TO DIAPHRAGM, NEAR RIGHT MEDIAN LOBE OF LIVER: SINGLE MASS, 40 X 38 X 15 MM, RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE MEDIASTINAL) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL DIAPHRAGM (DP) : -B-FIBROMA,-NEOPLASM PRESENT 1638 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 40 ANIMAL NUMBER: C90724 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 928.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:41 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1639 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 41 ANIMAL NUMBER: C90725 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 346.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:50 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 10.6740 ------------------------------------- ------------------------------- 3.0770 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1640 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 42 ANIMAL NUMBER: C90725 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 346.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:50 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1641 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 43 ANIMAL NUMBER: C90726 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 602.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1642 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 44 ANIMAL NUMBER: C90726 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 602.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 15 X 10 X 8 MM (LEFT IS 9 X 4 X 2 MM) (CAP 22) FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE DARK RED AND TAN ABRADED AREA, 13 X 12 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATELY SEVERE, MULTI FOCAL -EXOSTOSES,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 1643 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 45 ANIMAL NUMBER: C90726 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 602.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1644 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 46 ANIMAL NUMBER: C90727 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 486.9 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:49 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); PAW-FRONT-LEFT; WEEK 95; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); PAW-FRONT-RIGHT; WEEK 95; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN -CYST(S); BILATERAL, DIFFUSE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER ADRENAL, CORTEX (AC) : -MASS(ES); RIGHT: SINGLE MASS, 35 X 30 X 25 MM, RED, DARK RED, TAN, SOFT AND LOBULATED; CUT SURFACE CONTAINS A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (SEE RENAL) HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -ATROPHY, CORTICAL,-MODERATELY SEVERE, DIFFUSE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MODERATELY SEVERE, MULTI-FOCAL -NECROSIS, CORTICAL,-MODERATELY SEVERE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE 1645 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 47 ANIMAL NUMBER: C90727 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 486.9 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:49 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 15 X 6 MM; BOTH ARE SOFT LUNG (LU) : -MASS(ES); ALL LOBES: MULTIPLE MASSES, UP TO 7 X 5 X 5 MM, TAN, BROWN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (TRACHEOBRONCHIAL) NORMAL (CAP 22) STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 1 MM IN DIAMETER -THICKENED WALL; DIFFUSE TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-SLIGHT, MULTI FOCAL LUNG (LU) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE, MULTI-FOCAL -N-ADRENAL CORTICAL CARCINOMA,-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, GL (ST) : -MINERALIZATION,-MODERATE, MULTI-FOCAL 1646 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 48 ANIMAL NUMBER: C90727 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 486.9 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:49 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 7 X 3 MM LN, RENAL (RL) : -LARGE; LEFT: 10 X 10 X 5 MM (RIGHT IS 5 X 3 X 3 MM) (CAP 23) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL FRONT FEET: SINGLE DARK RED, TAN AND RAISED AREAS ON EACH, LEFT IS 20 X 5 X 5 MM, RIGHT IS 10 X 3 X 3 MM; BOTH COLLECTED AORTA (AO) : -LARGE; DIFFUSE: UP TO 9 MM IN DIAMETER; COLLECTED AORTIC ARCH (AA) : -LARGE; DIFFUSE: UP TO 10 MM IN DIAMETER; COLLECTED INTACT WITH HEART GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -ANIMAL THIN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -ADRENAL CORTICAL CARCINOMA,-PRESENT LN, RENAL (RL) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : ^UNREMARKABLE AORTA (AO) : -MINERALIZATION,-MODERATELY SEVERE, MULTI-FOCAL AORTIC ARCH (AA) : -MINERALIZATION,-MODERATELY SEVERE, MULTI-FOCAL 1647 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 49 ANIMAL NUMBER: C90727 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 486.9 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:49 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), PENIS (PE), AUDITORY SEB GL (AS), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, TRACHEOBRON (TB) 1648 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 50 ANIMAL NUMBER: C90728 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 890.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:16 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MASS(ES); ALONG MARGIN OF LEFT MEDIAN LOBE: SINGLE MASS, 13 X 12 X 10 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (HEPATIC) NOT IDENTIFIED -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 X 4 MM; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK BROWN FOCUS, 2 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -TELANGIECTASIS,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HYPERPLASIA, C-CELL,-SLIGHT, MULTI FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 1649 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 51 ANIMAL NUMBER: C90728 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 890.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:16 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1650 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 52 ANIMAL NUMBER: C90728 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 890.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:16 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER, OIL RED O (LI1), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1651 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 53 ANIMAL NUMBER: C90729 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/01/99 10:37 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; LEFT: 20 X 9 X 5 MM (RIGHT, SEE LARGE COMMENT); APPEARS SOFT -LARGE; RIGHT: 28 X 17 X 12 MM -RED/YELLOW FOCUS(I)/AREA(S); RIGHT, BENEATH TUNICA: MULTIPLE DARK RED AND YELLOW INDISTINCT AREAS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MODERATE, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 1652 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 54 ANIMAL NUMBER: C90729 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/01/99 10:37 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, DARK RED AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AREAS, UP TO 2 MM IN DIAMETER GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST, YELLOW MATERIAL -STAINS - SKIN/HAIRCOAT; INVOLVING ENTIRE HEAD: RED, MOIST MATERIAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MODERATE, MULTI-FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 1653 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 55 ANIMAL NUMBER: C90729 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/01/99 10:37 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SG), 1654 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 56 ANIMAL NUMBER: C90730 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 494.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 7:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MASS(ES); VISCERAL SURFACE OF LEFT LATERAL LOBE: TWO MASSES, ONE IS 20 X 15 X 5 MM, THE OTHER IS 8 X 5 X 3 MM; THE LARGER MASS IS DARK BROWN, RED, AND SOFT; SAME ON CUT SURFACE; THE SMALLER MASS IS RED AND SOFT; SAME ON CUT SURFACE; BOTH MASSES COLLECTED; REGIONAL LN (HEPATIC) NOT IDENTIFIED LN, MESENTERIC (MS) : -DIFFUSELY DARK; GREEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL 1655 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 57 ANIMAL NUMBER: C90730 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 494.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 7:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 15 X 12 X 8 MM, RED, DARK RED, TAN, GRAY, AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 1656 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 58 ANIMAL NUMBER: C90730 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 494.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 7:49 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SM), 1657 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 59 ANIMAL NUMBER: C90731 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 591.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:53 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0444 .0410 2.2260 1.9548 2.0881 18.2920 2.0427 .7911 1.7843 1.8109 .0107 .0156 .0075 % .0069 % .3766 % .3307 % .3533 % 3.0946 % .3456 % .1338 % .3019 % .3064 % .0018 % .0026 % OBSERVATIONS NECROPSY .0199 .0184 1.0000 .8782 .9381 8.2174 .9177 .3554 .8016 .8135 .0048 .0070 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN THYMUS (TH) : -MOTTLED; RIGHT LOBE: DARK RED AND TAN GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL PANCREAS (PA) : -FIBROSIS,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYMUS (TH) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1658 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 60 ANIMAL NUMBER: C90731 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 591.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:53 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1659 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 61 ANIMAL NUMBER: C90732 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 400.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:43 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 13.3343 ------------------------------------- ------------------------------- 3.3261 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1660 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 62 ANIMAL NUMBER: C90732 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 400.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:43 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1661 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 63 ANIMAL NUMBER: C90733 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); TAIL; WEEK 82; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -UNCOLLAPSED; ALL LOBES HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE, MULTI FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : >UNREMARKABLE HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL 1662 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 64 ANIMAL NUMBER: C90733 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TAIL (TI) : -RAISED AREA(S); ENTIRE LENGTH OF TAIL: MULTIPLE TAN AND SLIGHTLY RAISED AREAS, UP TO 15 X 10 X 2 MM; PORTION COLLECTED THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT -EROSION,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 1663 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 65 ANIMAL NUMBER: C90733 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 10:37 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1664 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 66 ANIMAL NUMBER: C90734 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 561.0 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 61; 20X20 MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; BILATERAL BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES, DIFFUSE: MULTIPLE WHITE AREAS, UP TO 2 X 3 MM HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -MINERALIZATION, CORNEAL,-MODERATE, FOCAL -ULCERATION, CORNEAL,-MODERATE, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE TESTIS (TE) : -INFLAMMATION, VASCULAR,-MINIMAL, MULTI FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL 1665 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 67 ANIMAL NUMBER: C90734 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 561.0 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 6 X 8 MM, FIRM, LOBULATED, RED, DARK RED, TAN, AND BROWN STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, PINPOINT AND UP TO 1 X 2 MM CECUM (CE) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED LINEAR AREAS, UP TO 4 X 7 MM HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MINIMAL, MULTI-FOCAL -MINERALIZATION,-MODERATELY SEVERE, MULTI-FOCAL -ULCERATION,-MODERATE, FOCAL CECUM (CE) : -EDEMA,-SLIGHT, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT 1666 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 68 ANIMAL NUMBER: C90734 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 561.0 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 4 X 5 X 8 MM, RIGHT IS 2 X 3 X 5 MM (CAP 23) -MOTTLED; BILATERAL: EACH IS RED, DARK RED, AND TAN (CAP 23) MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 13 X 17 X 24 MM, FIRM, LOBULATED, RED, AND TAN (CAP 21); SAME ON CUT SURFACE; REGIONAL LN (AXILLARY) NORMAL (CAP 22) AORTA (AO) : -LARGE; DIFFUSE: UP TO 4 MM IN DIAMETER -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -ANIMAL THIN LN, RENAL (RL) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL MAMMARY, MALE (MM) : -B-FIBROADENOMA,-NEOPLASM PRESENT AORTA (AO) : -MINERALIZATION,-SEVERE, MULTI-FOCAL 1667 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 69 ANIMAL NUMBER: C90734 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 561.0 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 1668 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 70 ANIMAL NUMBER: C90735 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:42 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1669 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 71 ANIMAL NUMBER: C90735 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:42 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1670 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 72 ANIMAL NUMBER: C90736 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 511.0 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 7:58 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL PITUITARY (PI) : -MASS(ES); SINGLE MASS, 19 X 7 X 7 MM, RED, DARK RED, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: TAN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL 1671 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 73 ANIMAL NUMBER: C90736 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 511.0 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 7:58 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE AND TAN, CREAMY, SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, FOCAL 1672 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 74 ANIMAL NUMBER: C90736 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 511.0 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 7:58 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1673 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 75 ANIMAL NUMBER: C90737 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 453.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:48 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0271 .0299 2.1502 1.3893 1.4361 12.9059 1.4966 .8485 1.4381 1.4800 .0090 .0084 .0060 % .0066 % .4743 % .3065 % .3168 % 2.8471 % .3302 % .1872 % .3173 % .3265 % .0020 % .0019 % OBSERVATIONS NECROPSY .0126 .0139 1.0000 .6461 .6679 6.0022 .6960 .3946 .6688 .6883 .0042 .0039 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: COLLECTED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >TISSUE MISSING 1674 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 76 ANIMAL NUMBER: C90737 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 453.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:48 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), DUODENUM (DU) 1675 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 77 ANIMAL NUMBER: C90738 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:58 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -CYST,-MODERATE, MULTI-FOCAL -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -OSSEOUS METAPLASIA,-MODERATE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1676 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 78 ANIMAL NUMBER: C90738 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:58 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -ANIMAL OBESE JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, FOCAL 1677 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 79 ANIMAL NUMBER: C90738 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:58 PROSECTOR: WENDY CHERRY RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1678 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 80 ANIMAL NUMBER: C90739 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 714 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 598.2 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 7:59 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS TESTIS (TE) : -SMALL; LEFT: 16 X 10 X 3 MM (RIGHT IS 20 X 15 X 5 MM); BOTH ARE SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE -MINERALIZATION,-SLIGHT, FOCAL >NOTE:>LEFT TESTIS HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE 1679 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 81 ANIMAL NUMBER: C90739 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 714 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 598.2 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 7:59 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 10 X 8 MM, RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 5 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: MOIST RED MATERIAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL PROSTATE (PR) : -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : -ONE EXAMINED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 1680 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 82 ANIMAL NUMBER: C90739 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/09/00 STUDY DAY OF DEATH: 714 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 598.2 GRAMS DATE AND TIME OF NECROPSY: 01/09/00 7:59 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1681 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 83 ANIMAL NUMBER: C90740 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/26/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 522.6 GRAMS DATE AND TIME OF NECROPSY: 04/26/99 14:59 PROSECTOR: WENDY CHERRY RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN PITUITARY (PI) : -MASS(ES);; 15 X 10 X 10 MM, DARK RED TAN, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S) ; MUCOSA: MULTIPLE BROWN AREAS, UP TO 1 X 1 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE ILEUM (IL) : -EDEMA,-MINIMAL, DIFFUSE PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL 1682 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 84 ANIMAL NUMBER: C90740 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/26/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 522.6 GRAMS DATE AND TIME OF NECROPSY: 04/26/99 14:59 PROSECTOR: WENDY CHERRY RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE DARK RED AND DRY AREAS, UP TO 5 X 4 MM; COLLECTED FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DARK RED AREA, 6 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE PRESENT ON THYMUS SECTION TAIL (TI) : -EROSION,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC ACTIVE,MODERATELY SEVERE, MULTI-FOCAL 1683 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 85 ANIMAL NUMBER: C90740 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/26/99 STUDY DAY OF DEATH: 456 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: 522.6 GRAMS DATE AND TIME OF NECROPSY: 04/26/99 14:59 PROSECTOR: WENDY CHERRY RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1684 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 86 ANIMAL NUMBER: C90741 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 13:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); VENTRAL-THORACICRIGHT; WEEK 69; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -ADHESION(S) ; ALL LOBES TO PLEURA: MULTIPLE GRAY FIBROUS ADHESIONS COLLECTED INTACT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL 1685 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 87 ANIMAL NUMBER: C90741 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 13:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 13 X 10 X 8 MM, DARK RED, RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 8 X 4 MM SKIN (SK) : -RAISED AREA(S); RIGHT VENTRAL THORACIC AREA: SINGLE TAN AREA, 7 X 7 X 5 MM (CAP 22) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: SINGLE TAN AND DRY AREA, 6 X 6 X 4 MM; COLLECTED; RIGHT NOT OBSERVED AT NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL 1686 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 88 ANIMAL NUMBER: C90741 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 497.3 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 13:51 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1687 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 89 ANIMAL NUMBER: C90742 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 778.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:21 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 1688 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 90 ANIMAL NUMBER: C90742 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 778.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:21 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1689 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 91 ANIMAL NUMBER: C90743 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1053.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:06 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS -CYST(S); ALONG MARGIN OF LATERAL LOBE: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER; COLLECTED HISTOPATHOLOGY LIVER (LI) : -CYST,-MODERATELY SEVERE, FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -HYPERPLASIA, ACINAR,-MODERATE, MULTI FOCAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT, FOCAL -VACUOLATION,-MINIMAL, FOCAL THYROID (TY) : -PIGMENT,-MINIMAL, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT 1690 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 92 ANIMAL NUMBER: C90743 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1053.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:06 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1691 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 93 ANIMAL NUMBER: C90743 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1053.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:06 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1692 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 94 ANIMAL NUMBER: C90744 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:22 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; LIMBSFRONT; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 7 X 11 MM, FIRM, RED, DARK RED AND TAN STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN AND RED ERODED AREAS, UP TO 2 X 3 MM -THICKENED WALL; DIFFUSE HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL KIDNEY (KD) : -INFARCT,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : >UNREMARKABLE PROSTATE (PR) : -FIBROSIS,-MODERATE, FOCAL 1693 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 95 ANIMAL NUMBER: C90744 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:22 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; HEAD AND FRONT LEGS: DRY RED MATERIAL -ANIMAL THIN PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1694 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 96 ANIMAL NUMBER: C90744 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:22 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1695 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 97 ANIMAL NUMBER: C90745 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:15 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >HOUSING,FOOD,WATER & MAINT.; TEETH CUT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 9 X 6 MM, DARK RED, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL 1696 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 98 ANIMAL NUMBER: C90745 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:15 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HIND FOOT: SINGLE, MOIST, BROWN ABRADED AREA, 7 MM IN DIAMETER (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 1697 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 99 ANIMAL NUMBER: C90745 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:15 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) , HEMATO NEOPLASIA (HN) 1698 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 100 ANIMAL NUMBER: C90746 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 551.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:19 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0373 .0361 2.1500 2.0638 2.1022 15.8931 1.7030 .8143 1.6455 1.7015 .0109 .0108 .0068 % .0065 % .3900 % .3744 % .3813 % 2.8828 % .3089 % .1477 % .2985 % .3086 % .0020 % .0020 % OBSERVATIONS NECROPSY .0173 .0168 1.0000 .9599 .9778 7.3921 .7921 .3787 .7653 .7914 .0051 .0050 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 1699 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 101 ANIMAL NUMBER: C90746 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 551.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:19 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), PARATHYROID (PT) 1700 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 102 ANIMAL NUMBER: C90747 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:48 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 97; 80 X 60MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -LARGE; 50 X 15 X 7 MM; MARGINS APPEAR ROUNDED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL,-MODERATE, FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL SPLEEN (SP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -HYPERTROPHY, CORTICAL CELL,-MODERATE, FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT TESTIS (TE) : >UNREMARKABLE 1701 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 103 ANIMAL NUMBER: C90747 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:48 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 8 X 7 MM; BOTH APPEAR SOFT PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, DARK RED AND SOFT LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 13 X 10 X 4 MM; COLLECTED LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SEMINAL VESICLES (SV) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT EPIDIDYMIDES (EP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1702 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 104 ANIMAL NUMBER: C90747 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:48 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 85 X 50 X 35 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, POPLITEAL (PP) : -LARGE; BILATERAL: EACH IS UP TO 10 X 8 X 8 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -ANIMAL THIN -BONE MARROW SMEAR TAKEN HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT MAMMARY, MALE (MM) : -B-FIBROADENOMA,-NEOPLASM PRESENT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, POPLITEAL (PP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 1703 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 105 ANIMAL NUMBER: C90747 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:48 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB) 1704 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 106 ANIMAL NUMBER: C90748 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 504.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:58 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0312 .0271 2.2436 1.8414 1.9977 15.6073 1.9152 .8258 1.4841 1.5291 .0137 .0119 .0062 % .0054 % .4447 % .3650 % .3960 % 3.0936 % .3796 % .1637 % .2942 % .3031 % .0027 % .0024 % OBSERVATIONS NECROPSY .0139 .0121 1.0000 .8207 .8904 6.9564 .8536 .3681 .6615 .6815 .0061 .0053 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1705 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 107 ANIMAL NUMBER: C90748 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 504.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:58 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1706 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 108 ANIMAL NUMBER: C90749 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 427.3 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 8:09 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN KIDNEY (KD) : -CYST(S); RIGHT, CORTICAL SURFACE: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER ADRENAL, CORTEX (AC) : -MOTTLED; BILATERAL: DARK RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CYST,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL 1707 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 109 ANIMAL NUMBER: C90749 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 427.3 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 8:09 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 5 X 4 MM, RED, TAN AND SOFT SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 17 X 9 X 3 MM MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE, CREAMY SEMIFLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: DRY, YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY, RED MATERIAL -ANIMAL THIN PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 1708 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 110 ANIMAL NUMBER: C90749 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 427.3 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 8:09 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1709 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 111 ANIMAL NUMBER: C90750 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:42 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); DORSAL; WEEK 105; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION LN, MESENTERIC (MS) : -LARGE; UP TO 15 MM IN DIAMETER -DIFFUSELY RED; CAUDAL END HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT, MULTI FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -NECROSIS,-MODERATE, MULTI-FOCAL -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL 1710 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 112 ANIMAL NUMBER: C90750 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:42 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -RAISED AREA(S); DORSAL THORACIC REGION: SINGLE DARK RED AND DRY AREA, 7 X 4 X 4 MM (CAP 23) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 12 X 8 X 6 MM (CAP 22) FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, HINDFEET: SINGLE DARK RED AND TAN ABRADED AREA ON EACH, LEFT IS 5 MM IN DIAMETER, RIGHT IS 4 MM IN DIAMETER; BOTH COLLECTED >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE AT TRIMMING GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : >TISSUE MISSING 1711 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 113 ANIMAL NUMBER: C90750 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:42 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1712 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 114 ANIMAL NUMBER: C90751 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 708.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0360 .0279 2.2280 2.2013 2.2651 21.2216 2.4259 1.1535 1.4942 1.5043 .0167 .0196 .0051 % .0039 % .3147 % .3109 % .3199 % 2.9974 % .3426 % .1629 % .2110 % .2125 % .0024 % .0028 % OBSERVATIONS NECROPSY .0162 .0125 1.0000 .9880 1.0167 9.5250 1.0888 .5177 .6706 .6752 .0075 .0088 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1713 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 115 ANIMAL NUMBER: C90751 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 708.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 1714 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 116 ANIMAL NUMBER: C90751 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 708.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:05 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD(SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1715 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 117 ANIMAL NUMBER: C90752 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 763.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST : DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0389 .0389 2.5855 2.6228 2.5076 21.9195 2.2547 .9130 1.5675 1.5833 .0157 .0185 .0051 % .0051 % .3386 % .3435 % .3284 % 2.8709 % .2953 % .1196 % .2053 % .2074 % .0021 % .0024 % OBSERVATIONS NECROPSY .0150 .0150 1.0000 1.0144 .9699 8.4779 .8721 .3531 .6063 .6124 .0061 .0072 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); BILATERAL: TWO DARK BROWN FOCI ON EACH, ALL ARE 1 MM IN DIAMETER LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL FOCAL -VACUOLATION,- MINIMAL, FOCAL 1716 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 118 ANIMAL NUMBER: C90752 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 763.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1717 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 119 ANIMAL NUMBER: C90752 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 763.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT),MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES),PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1718 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 120 ANIMAL NUMBER: C90753 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 345.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:20 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 10.4324 ------------------------------------- ------------------------------- 3.0178 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1719 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 121 ANIMAL NUMBER: C90753 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 345.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:20 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1720 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 122 ANIMAL NUMBER: C90754 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 789.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST : DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0315 .0301 2.5326 2.4118 2.4771 20.9861 2.1265 1.0063 1.7176 1.7539 .0179 .0257 .0040 % .0038 % .3207 % .3054 % .3137 % 2.6578 % .2693 % .1274 % .2175 % .2221 % .0023 % .0033 % OBSERVATIONS NECROPSY .0124 .0119 1.0000 .9523 .9781 8.2864 .8397 .3973 .6782 .6925 .0071 .0101 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 1721 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 123 ANIMAL NUMBER: C90754 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 789.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1722 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 124 ANIMAL NUMBER: C90755 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 13:33 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 1723 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 125 ANIMAL NUMBER: C90755 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 13:33 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 8 X 6 MM, DARK RED, GRAY, AND SOFT FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND FOOT: SINGLE TAN AND MOIST ABRADED AREA, 7 X 7 X 6 MM; COLLECTED GENERAL COMMENT (GC) : -ANIMAL OBESE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL 1724 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 126 ANIMAL NUMBER: C90755 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 13:33 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1725 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 127 ANIMAL NUMBER: C90756 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 386.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 11:00 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A T1 U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0348 .0326 2.1666 1.3350 1.2983 8.8391 1.3439 .4973 1.3216 1.3094 .0002 .0052 .0090 % .0084 % .5610 % .3457 % .3362 % 2.2887 % .3480 % .1288 % .3422 % .3390 % .0001 % .0013 % OBSERVATIONS NECROPSY .0161 .0150 1.0000 .6162 .5992 4.0797 .6203 .2295 .6100 .6044 .0001 .0024 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN EXCLUDE WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LUNG (LU) : -MOTTLED; TRIMMING COMMENT: LEFT LOBE CRANIAL HALF: RED AND TAN GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1726 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 128 ANIMAL NUMBER: C90756 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 386.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 11:00 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), DUODENUM (DU) 1727 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 129 ANIMAL NUMBER: C90757 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/28/99 STUDY DAY OF DEATH: 488 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 659.6 GRAMS DATE AND TIME OF NECROPSY: 05/28/99 7:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; SWOLLEN; LIMB-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -SMALL; LEFT: 13 X 9 X 7 MM (FOR COMPARISON, RIGHT IS 21 X 15 X 9 MM) LEFT IS SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE THYROID (TY) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 1728 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 130 ANIMAL NUMBER: C90757 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/28/99 STUDY DAY OF DEATH: 488 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 659.6 GRAMS DATE AND TIME OF NECROPSY: 05/28/99 7:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, RED ,DARK RED, GREY, TAN, LOBULATED AND SOFT STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE RED AND DARK RED AREAS, UP TO 3 X 2 MM -THICKENED WALL; MUCOSA STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA EPIDIDYMIDES (EP) : -SMALL; LEFT: 31 X 5 X 4 MM (FOR COMPARISON, RIGHT IS 51 X 8 X 7 MM) JOINT, OTHER (JT) : -LARGE; LEFT TARSAL AND METATARSAL: TARSAL IS 14 X 12 X 11 MM, METATARSAL IS 15 X 13 X 8 MM; TISSUE SURROUNDING BOTH APPEARS SWOLLEN; BOTH COLLECTED PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL EPIDIDYMIDES (EP) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT JOINT, OTHER (JT) : -DISLOCATION,-PRESENT -EDEMA,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE, SYNOVIAL,-MODERATE, MULTI-FOCAL 1729 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 131 ANIMAL NUMBER: C90757 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/28/99 STUDY DAY OF DEATH: 488 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 659.6 GRAMS DATE AND TIME OF NECROPSY: 05/2 8/99 7:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS, EXTENDING TO ABDOMINAL REGION: DRY, RED MATERIAL -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 1730 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 132 ANIMAL NUMBER: C90758 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:37 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE RED AREA, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 3 MM IN DIAMETER (CAP 22) PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PANCREAS (PA) : -HYPERTROPHY, VASCULAR,-MODERATE, MULTI FOCAL -INFLAMMATION, VASCULAR,-SLIGHT, MULTI FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : >UNREMARKABLE 1731 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 133 ANIMAL NUMBER: C90758 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:37 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1732 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 134 ANIMAL NUMBER: C90758 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:37 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1733 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 135 ANIMAL NUMBER: C90759 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 332.4 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND DARK RED PITUITARY (PI) : -MASS(ES); 10 X 10 X 9 MM, RED, DARK RED, TAN, GREY AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -LARGE; LEFT: 30 X 15 X 7 MM (RIGHT IS 20 X 10 X 5 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : >UNREMARKABLE SEMINAL VESICLES (SV) : >UNREMARKABLE 1734 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 136 ANIMAL NUMBER: C90759 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 332.4 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 1735 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 137 ANIMAL NUMBER: C90759 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 597 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 332.4 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1736 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 138 ANIMAL NUMBER: C90760 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 10:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >EYES; DISCOLORED/LIGHT RED; EYE-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -CALCULUS(I); RIGHT, WITHIN PELVIS: MULTIPLE TAN AND FIRM CALCULI, UP TO 10 X 6 X 5 MM -GRANULAR MATERIAL; LEFT, WITHIN PELVIS: MULTIPLE TAN PINPOINT GRANULES -LARGE; BILATERAL: EACH IS 35 X 24 X 16 MM -ADHESION(S); RIGHT, CORTICAL SURFACE TO RIGHT LATERAL AND CAUDATE LOBES OF LIVER: MULTIPLE GREY FIBROUS ADHESIONS; COLLECTED INTACT HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -NECROSIS, PAPILLARY,-SEVERE, DIFFUSE -PYELONEPHRITIS,-SEVERE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, MULTI-FOCAL -VACUOLATION,-SLIGHT, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT 1737 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 139 ANIMAL NUMBER: C90760 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 10:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); LEFT POSTERIOR ASPECT: SINGLE MASS, 3 MM IN DIAMETER, RED, DARK RED AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, PINPOINT AND UP TO 1 MM URINARY BLADDER (UB) : -THICKENED WALL; PROXIMAL END: TAN AND FIRM -CALCULUS(I); WITHIN LUMEN: MULTIPLE TAN CALCULI, UP TO 7 MM IN DIAMETER SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 8 X 2 MM LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL, MULTI-FOCAL URINARY BLADDER (UB) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE EPIDIDYMIDES (EP) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 1738 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 140 ANIMAL NUMBER: C90760 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 10:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: UP TO 20 X 15 X 4 MM (RIGHT IS NOT IDENTIFIED) (CAP 23) LN, RENAL (RL) : -LARGE; LEFT: 10 X 10 X 6 MM (RIGHT IS NOT IDENTIFIED) (CAP 22) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: SINGLE DARK RED CRUSTED AREA ON EACH, LEFT IS 12 X 10 MM, RIGHT IS 17 X 12 MM; BOTH COLLECTED GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; UP TO 30 MM IN DIAMETER; FILLED WITH A DARK BROWN FLUID CAUSE OF DEATH (CD) : -PYELONEPHRITIS,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL LN, RENAL (RL) : >TISSUE MISSING >NOTE:>RENAL LYMPH NODE DESCRIBED GROSSLY WAS ADIPOSE TISSUE FOOT/FOOTPAD(S) (FP) : -EROSION,-SEVERE, MULTI-FOCAL -EXOSTOSES,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL ADIPOSE TISSUE (AT) : -INFLAMMATION, CHRONIC ACTIVE,MODERATELY SEVERE, MULTI-FOCAL 1739 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 141 ANIMAL NUMBER: C90760 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 10:15 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), HEMATO NEOPLASIA (HN) (SM), 1740 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 142 ANIMAL NUMBER: C90761 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 662 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -MASS(ES); 6 X 4 X 3 MM, RED, GREY, TAN AND SOFT GENERAL COMMENT (GC) : -ANIMAL OBESE HISTOPATHOLOGY THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 1741 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 143 ANIMAL NUMBER: C90761 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/18/99 STUDY DAY OF DEATH: 662 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/18/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1742 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 144 ANIMAL NUMBER: C90762 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/27/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/27/99 8:12 PROSECTOR: LYNN HORNER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL HINDFEET: SINGLE DRY DARK RED ABRADED AREA ON EACH, RIGHT IS 8 X 6 MM, LEFT IS 5 X 4 MM; BOTH COLLECTED HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE FOOT/FOOTPAD(S) (FP) : -FIBROSIS,-SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL 1743 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 145 ANIMAL NUMBER: C90762 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/27/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/27/99 8:12 PROSECTOR: LYNN HORNER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1744 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 146 ANIMAL NUMBER: C90763 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 628.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEAD MAXILLARY-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; LIMITED USE; LIMBS-FRONT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL THYMUS (TH) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MODERATE, MULTI FOCAL 1745 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 147 ANIMAL NUMBER: C90763 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 628.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -ANIMAL THIN THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 1746 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 148 ANIMAL NUMBER: C90763 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 628.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 11:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1747 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 149 ANIMAL NUMBER: C90764 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/04/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/04/99 10:28 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 84; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 10 X 8 X 4 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT THYMUS (TH) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : >TISSUE MISSING >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS KERATOACANTHOMA 1748 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 150 ANIMAL NUMBER: C90764 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/04/99 STUDY DAY OF DEATH: 587 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/04/99 10:28 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) (PR), 1749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 151 ANIMAL NUMBER: C90765 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 888.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:59 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATELY SEVERE, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -INFLAMMATION, ACUTE,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -INFILTRATE, EOSINOPHILS,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : -THROMBUS,-SLIGHT, FOCAL 1750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 152 ANIMAL NUMBER: C90765 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 888.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:59 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE DARK BROWN AREA, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -B-PAPILLOMA, TRANSITIONAL CELL,NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 153 ANIMAL NUMBER: C90765 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 888.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:59 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SN), 1752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 154 ANIMAL NUMBER: C90766 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/10/99 STUDY DAY OF DEATH: 440 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 601.8 GRAMS DATE AND TIME OF NECROPSY: 04/10/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 9 X 9 MM, RED, DARK RED, GRAY, TAN AND SOFT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DRY, RED MATERIAL -BLOOD FILM TAKEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL LUNG (LU) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 1753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 155 ANIMAL NUMBER: C90766 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/10/99 STUDY DAY OF DEATH: 440 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 601.8 GRAMS DATE AND TIME OF NECROPSY: 04/10/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 156 ANIMAL NUMBER: C90767 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 710.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0366 .0340 2.2806 2.1832 2.2020 22.1916 1.7726 .9195 1.7098 1.6666 .0146 .0173 .0051 % .0048 % .3208 % .3071 % .3098 % 3.1221 % .2494 % .1294 % .2405 % .2345 % .0021 % .0024 % OBSERVATIONS NECROPSY .0160 .0149 1.0000 .9573 .9655 9.7306 .7773 .4032 .7497 .7308 .0064 .0076 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 157 ANIMAL NUMBER: C90767 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 710.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 158 ANIMAL NUMBER: C90767 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 710.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 159 ANIMAL NUMBER: C90768 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:25 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0276 .0281 2.2186 1.7442 1.7623 12.7081 1.7101 .4316 1.9313 1.8487 .0103 .0109 .0060 % .0061 % .4846 % .3810 % .3849 % 2.7759 % .3735 % .0943 % .4219 % .4038 % .0022 % .0024 % OBSERVATIONS NECROPSY .0124 .0127 1.0000 .7862 .7943 5.7280 .7708 .1945 .8705 .8333 .0046 .0049 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL LIVER (LI) : -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 1758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 160 ANIMAL NUMBER: C90768 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 457.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:25 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 161 ANIMAL NUMBER: C90769 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 460.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:41 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0272 .0247 2.2138 1.4498 1.4769 12.3579 1.6728 .7676 1.5958 1.5866 .0133 .0154 .0059 % .0054 % .4811 % .3150 % .3209 % 2.6853 % .3635 % .1668 % .3468 % .3448 % .0029 % .0033 % OBSERVATIONS NECROPSY .0123 .0112 1.0000 .6549 .6671 5.5822 .7556 .3467 .7208 .7167 .0060 .0070 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -CYST,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 162 ANIMAL NUMBER: C90769 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 460.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:41 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 163 ANIMAL NUMBER: C90770 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 379.4 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:10 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -SMALL; UP TO 3 MM IN DIAMETER THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : >UNREMARKABLE THYMUS (TH) : >UNREMARKABLE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SEVERE, FOCAL 1762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 164 ANIMAL NUMBER: C90770 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 379.4 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:10 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 14 X 8 X 8 MM, RED, DARK RED, GRAY, LOBULATED, AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, SURROUNDING PYLORIC VALVE: MULTIPLE TAN, AND SLIGHTLY ERODED AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL ABDOMINAL REGION: DRY BROWN MATERIAL -ANIMAL THIN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, FOCAL -ULCERATION,-MODERATE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 165 ANIMAL NUMBER: C90770 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 379.4 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:10 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES HEMATO NEOPLASIA (HN) (EP), 1764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 166 ANIMAL NUMBER: C90771 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 655 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 439.3 GRAMS DATE AND TIME OF NECROPSY: 11/11/99 7:16 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -DEPRESSED FOCUS(I)/AREA(S); RIGHT, CORTICAL SURFACE: SINGLE DARK BROWN DEPRESSED AREA, 3 X 2 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -INFARCT,-MINIMAL, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -INFLAMMATION, GRANULOMATOUS,-MODERATE, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 1765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 167 ANIMAL NUMBER: C90771 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 655 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 439.3 GRAMS DATE AND TIME OF NECROPSY: 11/11/99 7:16 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 8 X 5 MM, DARK RED, TAN, LOBULATED AND SOFT SEMINAL VESICLES (SV) : -SMALL; LEFT: 16 X 8 X 3 MM (RIGHT IS 21 X 11 X 5 MM) MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS AND RIGHT INGUINAL REGION: MULTIPLE WHITE, CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DRY, DARK RED CRUSTED AREA, 8 X 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 1766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 168 ANIMAL NUMBER: C90771 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/11/99 STUDY DAY OF DEATH: 655 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 439.3 GRAMS DATE AND TIME OF NECROPSY: 11/11/99 7:16 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DRY, RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 169 ANIMAL NUMBER: C90772 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/29/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 651.7 GRAMS DATE AND TIME OF NECROPSY: 03/29/99 11:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -MOTTLED; BILATERAL: BROWN AND TAN LUNG (LU) : -RED FOCUS(I)/AREA(S); DORSAL SURFACE OF LEFT LOBE: SINGLE DARK RED AREA, 6 X 22 MM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH HORN IS 8 X 14 X 32 MM -MOTTLED; BILATERAL: TAN, YELLOW AND RED LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL LUMBAR: EACH IS 12 X 12 X 27 MM (CAP 21) HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LUNG (LU) : >UNREMARKABLE MUSCLE, SKELETAL (SM) : -M-FIBROSARCOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -EDEMA,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL CAUSE OF DEATH (CD) : -FIBROSARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : >TISSUE MISSING 1768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 170 ANIMAL NUMBER: C90772 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/29/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 651.7 GRAMS DATE AND TIME OF NECROPSY: 03/29/99 11:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL LUMBAR: RED AND TAN (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY YELLOW MATERIAL -URINARY BLADDER-LARGE LUMEN; 20 X 20 X 32 MM, FILLED WITH A CLEAR YELLOW FLUID LN, LUMBAR/ILIAC (LM) : >NOTE:>LUMBAR LYMPH NODE DESCRIBED GROSSLY WAS A FIBROSARCOMA OF SKELETAL MUSCLE 1769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 171 ANIMAL NUMBER: C90772 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/29/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 651.7 GRAMS DATE AND TIME OF NECROPSY: 03/29/99 11:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD(SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID(TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX),CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT),ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 172 ANIMAL NUMBER: C90773 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/25/99 STUDY DAY OF DEATH: 455 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/25/99 8:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK RED, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, NEAR URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL OBESE -URINARY BLADDER-LARGE LUMEN; UP TO 19 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 1771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 173 ANIMAL NUMBER: C90773 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/25/99 STUDY DAY OF DEATH: 455 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/25/99 8:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 174 ANIMAL NUMBER: C90774 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 402.3 GRAMS DATE AND TIME OF NECROPSY: 06/09/99 14:00 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEAD MAXILLARY-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEAD MAXILLARY-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 8 X 5 MM, RED, DARK RED, TAN, LOBULATED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LUNG (LU) : -EDEMA, ALVEOLAR,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, FOCAL THYROID (TY) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 175 ANIMAL NUMBER: C90774 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 402.3 GRAMS DATE AND TIME OF NECROPSY: 06/09/99 14:00 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -DISCHARGE-NASAL; BILATERAL: DARK RED, DRY MATERIAL -ANIMAL THIN MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 1774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 176 ANIMAL NUMBER: C90774 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 402.3 GRAMS DATE AND TIME OF NECROPSY: 06/09/99 14:00 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 177 ANIMAL NUMBER: C90775 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 352.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:53 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 10.6997 ------------------------------------- ------------------------------- 3.0319 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 178 ANIMAL NUMBER: C90775 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 352.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:53 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 179 ANIMAL NUMBER: C90776 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 7:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : >TISSUE MISSING HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC 1778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 180 ANIMAL NUMBER: C90776 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 7:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -ANIMAL OBESE ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 1779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 181 ANIMAL NUMBER: C90776 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 7:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 182 ANIMAL NUMBER: C90777 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/29/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 482.9 GRAMS DATE AND TIME OF NECROPSY: 09/29/99 8:08 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRALTHORACIC; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES) ; SINGLE MASS, 11 X 10 X 9 MM, RED, DARK RED, TAN, LOBULATED, AND FIRMrpT tdi\/i HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 1781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 183 ANIMAL NUMBER: C90777 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/29/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 482.9 GRAMS DATE AND TIME OF NECROPSY: 09/29/99 8:08 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 40 X 20 X 5 MM FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE TAN, MOIST ABRADED AREA, 9 X 8 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, GL (ST) : -EROSION,-MINIMAL, FOCAL STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 1782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 184 ANIMAL NUMBER: C90777 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/29/99 STUDY DAY OF DEATH: 612 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 482.9 GRAMS DATE AND TIME OF NECROPSY: 09/29/99 8:08 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND(HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 185 ANIMAL NUMBER: C90778 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:41 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 82; 90 X 80MM; VERIFIED AT NECROPSY: OBSERVED >EYES; OPAQUE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; BILATERAL: CLEAR PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK BROWN AREA, 3 X 1 MM HISTOPATHOLOGY EYE (EY) : -CATARACT,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 1784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 186 ANIMAL NUMBER: C90778 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:41 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -LARGE; RIGHT: 25 X 15 X 5 MM (LEFT IS 20 X 10 X 4 MM); FIRM MAMMARY, MALE (MM) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 120 X 90 X 40 MM, RED, DARK RED, TAN, GREY, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-MINIMAL, MULTI FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS ON THYMUS SECTION MAMMARY, MALE (MM) : -NECROSIS,-MODERATELY SEVERE, MULTI FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 1785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 187 ANIMAL NUMBER: C90778 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 707.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:41 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 188 ANIMAL NUMBER: C90779 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 442.5 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; OPAQUE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; RIGHT BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, GRAY, TAN, LOBULATED AND SOFT HISTOPATHOLOGY EYE (EY) : -MINERALIZATION, LENS,-MINIMAL, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 1787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 189 ANIMAL NUMBER: C90779 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 442.5 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM -THICKENED WALL; MUCOSA STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA PROSTATE (PR) : -NODULAR; PROXIMAL, TISSUE SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR AND SOFT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: BROWN MOIST MATERIAL -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY RED MATERIAL -ANIMAL THIN -GI TRACT-LARGE LUMEN; INVOLVING ENTIRE GI TRACT: UP TO 18 MM IN DIAMETER; DISTENDED WITH GAS SALIV GL, MANDIB (SG) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL STOMACH, NONGL (SU) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, FOCAL -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 190 ANIMAL NUMBER: C90779 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 442.5 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 14:02 PROSECTOR: JOHN S. HALFORD RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES HEMATO NEOPLASIA (HN) (EP), 1789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 191 ANIMAL NUMBER: C90780 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 574.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:32 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0459 .0441 2.2694 2.2515 2.3423 17.4975 1.9971 .7631 1.6424 1.6309 .0171 .0109 .0080 % .0077 % .3949 % .3918 % .4076 % 3.0446 % .3475 % .1328 % .2858 % .2838 % .0030 % .0019 % OBSERVATIONS NECROPSY .0202 .0194 1.0000 .9921 1.0321 7.7102 .8800 .3363 .7237 .7186 .0075 .0048 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK RED AREA, 3 X 1 MM PROSTATE (PR) : -LARGE; 25 X 15 X 15 MM SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 28 X 15 X 3 MM GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL PROSTATE (PR) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL SEMINAL VESICLES (SV) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 192 ANIMAL NUMBER: C90780 SEX: MALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 574.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:32 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 193 ANIMAL NUMBER: C90781 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:45 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, GRAY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-MINIMAL, MULTI FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE, MULTI FOCAL -MINERALIZATION,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 194 ANIMAL NUMBER: C90781 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:45 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) 1793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 195 ANIMAL NUMBER: C90782 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 664.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); DORSAL; WEEK 105; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS TESTIS (TE) : -SMALL; BILATERAL: EACH IS 13 X 7 X 4 MM; BOTH APPEAR SOFT HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT 1794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 196 ANIMAL NUMBER: C90782 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 664.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -MASS(ES); DORSAL THORACIC REGION: SINGLE MASS, 10 MM IN DIAMETER, TAN, FIRM AND FRIABLE; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED AREA, 10 MM IN DIAMETER; SURROUNDING TISSUE APPEARS SWOLLEN; COLLECTED LN, POPLITEAL (PP) : -LARGE; RIGHT: 12 X 8 X 7 MM (LEFT IS NOT IDENTIFIED) (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL LN, POPLITEAL (PP) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, DIFFUSE 1795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 197 ANIMAL NUMBER: C90782 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 664.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB), LN, AXILLARY (AX) 1796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 198 ANIMAL NUMBER: C90783 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 657.2 GRAMS DATE AND TIME OF NECROPSY: 10/26/00 8:02 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DARK RED AND MOIST ABRADED AREA, 13 X 9 MM; APPEARS SWOLLEN; COLLECTED LN, POPLITEAL (PP) : -LARGE; LEFT: 10 X 8 X 4 MM (RIGHT IS 3 X 2 X 1 MM) (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL LN, POPLITEAL (PP) : >UNREMARKABLE 1797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 199 ANIMAL NUMBER: C90783 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 657.2 GRAMS DATE AND TIME OF NECROPSY: 10/26/00 8:02 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), TESTIS (TE), LUNG (LU) 1798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 200 ANIMAL NUMBER: C90784 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 454.0 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -ROUGH SURFACE; DIFFUSE PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, LOBULATED AND SOFT LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 10 X 7 X 6 MM (LEFT IS 2 X 1 X 1 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL SPLEEN (SP) : >UNREMARKABLE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 1799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 201 ANIMAL NUMBER: C90784 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 454.0 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DRY, DARK RED CRUSTED AREA, 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN FOOT/FOOTPAD(S) (FP) : -EROSION,-SLIGHT, FOCAL -FIBROSIS,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL 1800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 202 ANIMAL NUMBER: C90784 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 454.0 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 203 ANIMAL NUMBER: C90785 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 89; 55 X 65MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); TAIL; WEEK 104; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -TELANGIECTASIS,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MINIMAL, MULTI FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE, DIFFUSE STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL 1802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 204 ANIMAL NUMBER: C90785 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 45 X 35 X 25 MM, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -LARGE; RIGHT: 10 X 6 X 3 MM (LEFT IS 3 X 2 X 1 MM) -MOTTLED; BILATERAL: RED AND TAN (CAP 22) TAIL (TI) : -CRUSTED AREA(S); ENTIRE LENGTH: MULTIPLE DARK BROWN AND RAISED AREAS, UP TO 6 MM IN DIAMETER; PORTION COLLECTED FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK BROWN AREA, 5 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, NONGL (SU) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS LIPOMA. LN, AXILLARY (AX) : >UNREMARKABLE TAIL (TI) : -EROSION,-SEVERE, FOCAL -INFLAMMATION, ACUTE,-SEVERE, FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-SEVERE, FOCAL 1803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 205 ANIMAL NUMBER: C90785 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) 1804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 206 ANIMAL NUMBER: C90786 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/02/99 14:11 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED FOCI, UP TO 1 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT 1805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 207 ANIMAL NUMBER: C90786 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/02/99 14:11 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 1806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 208 ANIMAL NUMBER: C90787 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 652.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:59 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED FOCI, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 209 ANIMAL NUMBER: C90787 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 652.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:59 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: UP TO 12 X 8 X 5 MM (CAP 22) JOINT, OTHER (JT) : -LARGE; BILATERAL, TARSAL JOINTS: EACH IS UP TO 15 X 11 X 11 MM; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL JOINT, OTHER (JT) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EXOSTOSES,-MODERATELY SEVERE, MULTI FOCAL -FIBROSIS,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 1808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 210 ANIMAL NUMBER: C90787 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 652.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:59 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE) 1809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 211 ANIMAL NUMBER: C90788 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:04 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM, COLLECTED ON ROUTINE SECTIONS LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE HISTOPATHOLOGY LIVER (LI) : -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 1810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 212 ANIMAL NUMBER: C90788 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:04 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) 1811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 213 ANIMAL NUMBER: C90789 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 407.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:37 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 12.0305 ------------------------------------- ------------------------------- 2.9501 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 214 ANIMAL NUMBER: C90789 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 407.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:37 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 215 ANIMAL NUMBER: C90790 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 637.6 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 12:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 12 MM, SOFT, RED, DARK RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 216 ANIMAL NUMBER: C90790 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 637.6 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 12:45 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: MOIST YELLOW MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 217 ANIMAL NUMBER: C90791 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 757.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:28 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL,-MODERATELY SEVERE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 218 ANIMAL NUMBER: C90791 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 757.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:28 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY) 1817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 219 ANIMAL NUMBER: C90792 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/99 STUDY DAY OF DEATH: 396 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/99 12:04 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, DARK RED, TAN, GRAY AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE ULCERATED AREAS, 1 MM IN DIAMETER ILEUM (IL) : >NOTE:>TRIMMING COMMENT: MISSING AT TIME OF TRIM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -EROSION,-MINIMAL, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL ILEUM (IL) : >TISSUE MISSING CECUM (CE) : >TISSUE MISSING 1818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 220 ANIMAL NUMBER: C90792 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/99 STUDY DAY OF DEATH: 396 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/99 12:04 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CECUM (CE) : >NOTE:>TRIMMING COMMENT: MISSING AT TIME OF TRIM COLON (CO) : >NOTE:>TRIMMING COMMENT: MISSING AT TIME OF TRIM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN COLON (CO) : >TISSUE MISSING PROSTATE (PR) : -EDEMA,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 221 ANIMAL NUMBER: C90792 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/99 STUDY DAY OF DEATH: 396 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 474.7 GRAMS DATE AND TIME OF NECROPSY: 02/25/99 12:04 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 222 ANIMAL NUMBER: C90793 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 12:18 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, RED, DARK RED, GRAY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MODERATE, MULTI FOCAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-MODERATE, FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, FOCAL 1821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 223 ANIMAL NUMBER: C90793 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 12:18 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 224 ANIMAL NUMBER: C90794 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 759.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, ANT MES/PANC (AP) : -LARGE; UP TO 5 MM IN DIAMETER (CAP 22) -DIFFUSELY RED; DARK RED LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: UP TO 19 X 11 X 10 MM (CAP 23) HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -INFARCT,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, ANT MES/PANC (AP) : >TISSUE MISSING >NOTE:>PANCREATIC LYMPH NODE DESCRIBED GROSSLY WAS A ISLET CELL ADENOMA OF THE PANCREAS LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL 1823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 225 ANIMAL NUMBER: C90794 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 759.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED AND TAN AREA, 10 X 9 MM; APPEARS SWOLLEN; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE BROWN AND FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, MULTI FOCAL -EXOSTOSES,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 1824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 226 ANIMAL NUMBER: C90794 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 759.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 1825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 227 ANIMAL NUMBER: C90795 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 431.6 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; GENERALIZED; VERIFIED AT NECROPSY: NOT OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK RED, TAN, GRAY AND SOFT STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA, DIFFUSE RECTUM (RE) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE, CILIARY,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL RECTUM (RE) : >TISSUE MISSING 1826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 228 ANIMAL NUMBER: C90795 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 431.6 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -CYST(S); BILATERAL AXILLARY REGIONS: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 21) PENIS (PE) : -PROTRUDING; APPROXIMATELY 9 MM EXPOSED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; BILATERAL: DRY RED MATERIAL PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL PENIS (PE) : -PROLAPSE,-PRESENT PREPUTIAL GLAND (PG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL >NOTE:>PREPUTIAL GLAND WAS PRESENT ON PENIS SLIDE 1827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 229 ANIMAL NUMBER: C90795 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 431.6 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 230 ANIMAL NUMBER: C90796 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 412 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 16:23 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, RED, TAN, DARK RED AND FIRM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 2 X 1 MM PROSTATE (PR) : -NODULAR; TISSUE PROXIMAL TO URETHRA: GREEN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL 1829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 231 ANIMAL NUMBER: C90796 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/13/99 STUDY DAY OF DEATH: 412 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/13/99 16:23 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 232 ANIMAL NUMBER: C90797 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 533.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:29 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0263 .0244 2.2572 1.7313 1.7203 13.8052 1.7917 .8018 1.7172 1.8086 .0090 .0183 .0049 % .0046 % .4232 % .3246 % .3225 % 2.5882 % .3359 % .1503 % .3219 % .3391 % .0017 % .0034 % OBSERVATIONS NECROPSY .0117 .0108 1.0000 .7670 .7621 6.1161 .7938 .3552 .7608 .8013 .0040 .0081 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: TWO PINPOINT FOCI GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, FOCAL STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 233 ANIMAL NUMBER: C90797 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 533.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:29 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (SE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 234 ANIMAL NUMBER: C90798 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 746.3 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 14:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AND LINEAR AREA, 7 X 2 MM; COLLECTED ON ROUTINE SECTION -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE DARK RED AREA, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY EYE (EY) : -VASCULARIZATION, CORNEAL,-MINIMAL, MULTI-FOCAL LIVER (LI) : -FIBROSIS, CAPSULAR,-MINIMAL, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SEVERE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -TELANGIECTASIS,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 1833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 235 ANIMAL NUMBER: C90798 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 746.3 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 14:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE TAN, DRY, AND SLIGHTLY RAISED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HYPERPLASIA, C-CELL,-MINIMAL, FOCAL PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL 1834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 236 ANIMAL NUMBER: C90798 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 746.3 GRAMS DATE AND TIME OF NECROPSY: 12/29/99 14:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 237 ANIMAL NUMBER: C90799 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 685.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:17 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -MASS(ES); VISCERAL SURFACE OF LEFT LATERAL LOBE: SINGLE MASS, 15 X 10 X 8 MM, RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -HYPERPLASIA, ACINAR,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 238 ANIMAL NUMBER: C90799 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 685.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:17 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) 1837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 239 ANIMAL NUMBER: C90800 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/26/99 11:21 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -CALCULUS(I); RIGHT, WITHIN PELVIS: SINGLE TAN, HARD AND FLAT CALCULUS, 4 X 2 X LESS THAN 1 MM -LARGE; BILATERAL: EACH IS 30 X 20 X 16 MM ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 13 X 8 X 8 MM (LEFT IS 5 X 4 X 4 MM) LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE, FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : >UNREMARKABLE HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL 1838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 240 ANIMAL NUMBER: C90800 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/26/99 11:21 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: DARK BROWN AND TAN (CAP 21) THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL 1839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 241 ANIMAL NUMBER: C90800 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/26/99 11:21 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 242 ANIMAL NUMBER: C90801 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 9:35 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); LIMB-HIND-RIGHT; WEEK 98; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 15 MM IN DIAMETER (CAP 22) -MOTTLED; ALL LOBES: RED, DARK RED, DARK BROWN AND TAN PITUITARY (PI) : -LARGE; 5 X 5 X 4 MM; APPEARS SWOLLEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 1841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 243 ANIMAL NUMBER: C90801 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 9:35 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, ANT MES/PANC (AP) : -LARGE; ANTERIOR MESENTERIC: 7 MM IN DIAMETER (CAP 23) MAMMARY, MALE (MM) : -MASS(ES); RIGHT LATERAL ABDOMINAL REGION, EXTENDING TO RIGHT LATERAL HINDLEG: SINGLE MASS, 45 X 30 X 20 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT LN, ANT MES/PANC (AP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, MALE (MM) : >TISSUE MISSING >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS HISTIOCYTIC SARCOMA IN THE SKIN 1842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 244 ANIMAL NUMBER: C90801 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 9:35 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 1843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 245 ANIMAL NUMBER: C90802 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/10/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 435.8 GRAMS DATE AND TIME OF NECROPSY: 08/10/99 13:58 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: NOT FOUND AT TRIMMING MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: NOT FOUND AT TRIMMING HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL BONE, FEMUR (FE) : >TISSUE MISSING MARROW, FEMUR (FM) : >TISSUE MISSING THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 246 ANIMAL NUMBER: C90802 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/10/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 435.8 GRAMS DATE AND TIME OF NECROPSY: 08/10/99 13:58 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X8 MM, SOFT, RED, AND DARK RED STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN AND RED ERODED AREAS, UP TO 2 X 3 MM -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -ANIMAL THIN STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, MULTI-FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 247 ANIMAL NUMBER: C90802 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/10/99 STUDY DAY OF DEATH: 562 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 435.8 GRAMS DATE AND TIME OF NECROPSY: 08/10/99 13:58 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SG), 1846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 248 ANIMAL NUMBER: C90803 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 651 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/07/99 8:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; OPAQUE; EYES; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); TAIL; WEEK 93; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; BILATERAL LIVER (LI) : -LARGE; ALL LOBES KIDNEY (KD) : -LARGE; BILATERAL: EACH IS 29 X 21 X 19 MM TESTIS (TE) : -SMALL; BILATERAL: EACH IS 12 X 7 X 6 MM LUNG (LU) : -UNCOLLAPSED; ALL LOBES HISTOPATHOLOGY EYE (EY) : -FIBROSIS, LENS,-MINIMAL, FOCAL LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MODERATE, MULTI FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT 1847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 249 ANIMAL NUMBER: C90803 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 651 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/07/99 8:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 9 X 5 MM TAIL (TI) : -RAISED AREA(S); 41 MM FROM BASE: SINGLE DRY, TAN RAISED AREA, 3 X 3 X 2 MM; COLLECTED ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT 1848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 250 ANIMAL NUMBER: C90803 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/07/99 STUDY DAY OF DEATH: 651 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/07/99 8:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SG), 1849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 251 ANIMAL NUMBER: C90804 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:35 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >EYES; OPAQUE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; BILATERAL LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AND SLIGHTLY DEPRESSED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN -CYST(S); BILATERAL, DIFFUSE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE, CILIARY,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE, CORNEAL,MODERATE, MULTI-FOCAL -ULCERATION, CORNEAL,-MODERATE, MULTI FOCAL -VASCULARIZATION, CORNEAL,-MODERATE, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -CYST,-SEVERE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE 1850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 252 ANIMAL NUMBER: C90804 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:35 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 2 X 2 X 1 MM STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC REGION: SINGLE DARK RED AND DRY AREA, 4 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -EDEMA,-MODERATE, FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 253 ANIMAL NUMBER: C90804 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:35 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY) 1852 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 254 ANIMAL NUMBER: C90805 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/24/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 457.0 GRAMS DATE AND TIME OF NECROPSY: 03/24/99 7:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; SCROTUM; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -SMALL; BILATERAL: EACH IS 15 X 8 X 4 MM; BOTH ARE SOFT PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL -EROSION,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 1853 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 255 ANIMAL NUMBER: C90805 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/24/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 457.0 GRAMS DATE AND TIME OF NECROPSY: 03/24/99 7:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH I S 1 0 X 5 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN SEMINAL VESICLES (SV) : ^UNREMARKABLE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1854 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 256 ANIMAL NUMBER: C90805 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/24/99 STUDY DAY OF DEATH: 423 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 457.0 GRAMS DATE AND TIME OF NECROPSY: 03/24/99 7:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 1855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 257 ANIMAL NUMBER: C90806 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 239 STUDY WEEK OF DEATH: 35 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 14:20 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 21; 95 X 100MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: LIGHT TAN LUNG (LU) : -ADHESION(S); TRIM COMMENT: LEFT LOBE ADHERED TO DIAPHRAGM: SINGLE TAN AND FIBROUS ADHESION, 8 MM IN LENGTH LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT, LUMBAR: 7 X 3 X 1 MM (LEFT NOT IDENTIFIED) (CAP 21) HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL SKIN (SK) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -NECROSIS,-SEVERE, MULTI-FOCAL -M-SARCOMA, UNDIFFERENTIATED,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -UNDIFFERENTIATED SARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : >UNREMARKABLE MAMMARY, MALE (MM) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>MAMMARY GLAND MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS FIBROMA 1856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 258 ANIMAL NUMBER: C90806 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 239 STUDY WEEK OF DEATH: 35 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 14:20 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); EXTENDING FROM RIGHT TO LEFT INGUINAL REGION AND XIPHOID PROCESS TO PERIANAL REGION: SINGLE MASS, 120 X 110 X 60 MM (340.4 GRAMS), RED, TAN, DARK RED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED OVERLYING SKIN HAS A SINGLE RED, DARK RED, CRUSTED AREA, 80 X 60 MM; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN 1857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 259 ANIMAL NUMBER: C90806 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/98 STUDY DAY OF DEATH: 239 STUDY WEEK OF DEATH: 35 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/21/98 14:20 PROSECTOR: JAMES T. CALLEN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 260 ANIMAL NUMBER: C90807 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 522.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:41 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0231 .0207 2.2198 1.8047 1.9042 14.8853 1.7490 .8167 1.8401 1.8383 .0109 .0155 .0044 % .0040 % .4252 % .3457 % .3648 % 2.8516 % .3351 % .1565 % .3525 % .3522 % .0021 % .0030 % OBSERVATIONS NECROPSY .0104 .0093 1.0000 .8130 .8578 6.7057 .7879 .3679 .8289 .8281 .0049 .0070 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 261 ANIMAL NUMBER: C90807 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 522.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:41 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 262 ANIMAL NUMBER: C90808 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 398.6 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:14 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -MOTTLED; BILATERAL: DARK BROWN AND TAN PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, RED, DARK RED, GRAY, TAN, LOBULATED, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 3 X 2 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CYST,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 1861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 263 ANIMAL NUMBER: C90808 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 398.6 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:14 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR AND SOFT LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR LN: EACH IS 6 X 4 X 3 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : ^UNREMARKABLE 1862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 264 ANIMAL NUMBER: C90808 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 398.6 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:14 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 265 ANIMAL NUMBER: C90809 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 717.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 6 MM IN DIAMETER (LEFT NOT IDENTIFIED); RIGHT APPEARS CYSTIC (CAP 23) HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -INFLAMMATION, GRANULOMATOUS,-SLIGHT, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL 1864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 266 ANIMAL NUMBER: C90809 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 717.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU) 1865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 267 ANIMAL NUMBER: C90810 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 910.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -DARK FOCUS(I)/AREA(S); RIGHT CORTEX: SINGLE BLACK AREA, 4 MM IN DIAMETER ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); RIGHT: SINGLE DARK BROWN AREA, 2 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATELY SEVERE, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -TELANGIECTASIS,-MODERATE, FOCAL KIDNEY (KD) : -CYST,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -HYPERPLASIA, ACINAR,-MINIMAL, MULTI FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL 1866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 268 ANIMAL NUMBER: C90810 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 910.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); POSTERIOR ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MODERATE, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 269 ANIMAL NUMBER: C90810 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 910.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) 1868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 270 ANIMAL NUMBER: C90811 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 842.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT JUNCTION OF RIGHT AND LEFT MEDIAN LOBES: SINGLE YELLOW FOCUS, 1 MM IN DIAMETER; COLLECTED -RED FOCUS(I)/AREA(S); ALL LOBES, DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -INFARCT,-SLIGHT, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PANCREAS (PA) : -CYST,-MINIMAL, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 1869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 271 ANIMAL NUMBER: C90811 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 842.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : >UNREMARKABLE URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 272 ANIMAL NUMBER: C90812 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 502.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS MUSCLE, SKELETAL (SM) : -ATROPHY-FOCAL; BILATERAL HIND LEGS; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -PIGMENT,-SLIGHT, FOCAL -TELANGIECTASIS,-MODERATE, FOCAL -THROMBUS,-SLIGHT, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 273 ANIMAL NUMBER: C90812 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 502.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, GRAY, TAN AND SOFT SKIN (SK) : -CRUSTED AREA(S); LEFT LATERAL THORACIC REGION: SINGLE TAN, DRY, LOBULATED AND CRUSTED AREA, 5 MM IN DIAMETER (CAP 22) PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM ADIPOSE TISSUE (AT) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 25 X 15 X 10 MM, WHITE, SOFT AND GELATINOUS; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (MANDIBULAR) NORMAL (CAP 24) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: YELLOWISH-BROWN AND MOIST -BLOOD FILM TAKEN -DISCHARGE-NASAL; DARK RED AND DRY -STAINS - SKIN/HAIRCOAT; ABDOMINAL REGION: YELLOW AND DRY -BONE MARROW SMEAR TAKEN SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT ADIPOSE TISSUE (AT) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -B-LIPOMA,-NEOPLASM PRESENT 1872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 274 ANIMAL NUMBER: C90812 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 502.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (MS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB), LN, MANDIBULAR (MN) 1873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 275 ANIMAL NUMBER: C90813 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/18/98 STUDY DAY OF DEATH: 52 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 339.5 GRAMS DATE AND TIME OF NECROPSY: 03/18/98 14:18 PROSECTOR: NANCY DIEDRICH RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >DISCHARGE; ORAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LUNG (LU) : -DIFFUSELY RED; CRANIAL REGIONS OF ALL LOBES: DARK RED CAVITY, ORAL (OC) : -FRACTURE(S); EXTENDING FROM HARD PALATE TO NASAL BONE: SINGLE FRACTURE, 5 MM IN WIDTH; SURROUNDING TISSUE DARK RED; COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK RED AND CRUSTED -STAINS-PERINEUM/PERIANAL; DARK RED AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; DARK RED AND CRUSTED -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ABDOMEN: DARK RED AND DRY HISTOPATHOLOGY LUNG (LU) : ^UNREMARKABLE HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -FRACTURE, HARD PALATE,-PRESENT CAVITY, ORAL (OC) : -BACTERIAL COLONIES,-PRESENT -FRACTURE,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 1874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 276 ANIMAL NUMBER: C90813 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/18/98 STUDY DAY OF DEATH: 52 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 339.5 GRAMS DATE AND TIME OF NECROPSY: 03/18/98 14:18 PROSECTOR: NANCY DIEDRICH RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 277 ANIMAL NUMBER: C90814 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 323.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:13 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 9.3873 ------------------------------------- ------------------------------- 2.9018 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 278 ANIMAL NUMBER: C90814 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 323.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:13 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 279 ANIMAL NUMBER: C90815 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:18 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 76; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SPLEEN (SP) : -LARGE; 5 0 X 1 0 X 5 MM SKIN (SK) : -MASS(ES); DORSAL THORACIC REGION: SINGLE MASS, 25 X 20 X 10 MM, RED, TAN AND GELATINOUS; SAME ON CUT SURFACE; OVERLYING SURFACE IS DARK RED AND ABRADED; COLLECTED; REGIONAL LN (SEE AXILLARY) HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL SPLEEN (SP) : >UNREMARKABLE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 280 ANIMAL NUMBER: C90815 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 638.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:18 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; RIGHT: UP TO 12 X 6 X 4 MM (LEFT IS UP TO 5 X 3 X 2 MM) (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), THYROID (TY), PARATHYROID (PT) 1879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 281 ANIMAL NUMBER: C90816 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 565.0 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, RED, GRAY, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HEMORRHAGE,-MINIMAL, FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 282 ANIMAL NUMBER: C90816 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 565.0 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SV), 1881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 283 ANIMAL NUMBER: C90817 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 341.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:00 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 9.6870 ------------------------------------- ------------------------------- 2.8366 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 284 ANIMAL NUMBER: C90817 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 341.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:00 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 285 ANIMAL NUMBER: C90818 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 515.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:27 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0377 .0320 2.1395 2.1015 2.0749 16.4851 1.7990 .8540 1.7136 1.8148 .0145 .0084 .0073 % .0062 % .4150 % .4077 % .4025 % 3.1979 % .3490 % .1657 % .3324 % .3520 % .0028 % .0016 % OBSERVATIONS NECROPSY .0176 .0150 1.0000 .9822 .9698 7.7051 .8409 .3992 .8009 .8482 .0068 .0039 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 286 ANIMAL NUMBER: C90818 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 515.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:27 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 287 ANIMAL NUMBER: C90819 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 14:10 PROSECTOR: JARED FISHEL RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : -COLLAPSED GLOBE-ANTE MORTEM; RED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL LEFT: HISTOPATHOLOGY EYE (EY) : ^UNREMARKABLE BRAIN (BR) : >UNREMARKABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, DIFFUSE PANCREAS (PA) : -INFLAMMATION, VASCULAR,-SLIGHT, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL 1886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 288 ANIMAL NUMBER: C90819 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 14:10 PROSECTOR: JARED FISHEL RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 5 MM IN DIAMETER, DARK RED, GREY, AND SOFT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT EPIDIDYMIDES (EP) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 1887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 289 ANIMAL NUMBER: C90819 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/99 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/99 14:10 PROSECTOR: JARED FISHEL RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 1888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 290 ANIMAL NUMBER: C90820 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/25/99 STUDY DAY OF DEATH: 485 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 648.3 GRAMS DATE AND TIME OF NECROPSY: 05/25/99 12:49 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 10 X 9 MM, RED, DARK RED, GREY, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 291 ANIMAL NUMBER: C90820 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/25/99 STUDY DAY OF DEATH: 485 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 648.3 GRAMS DATE AND TIME OF NECROPSY: 05/25/99 12:49 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 292 ANIMAL NUMBER: C90821 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; OPAQUE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; LEFT PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : >UNREMARKABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MINIMAL, FOCAL -VACUOLATION,-MINIMAL, FOCAL THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 293 ANIMAL NUMBER: C90821 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: SINGLE DARK RED AND DRY AREA, 12 X 10 X 7 MM; COLLECTED GENERAL COMMENT (GC) : -ANIMAL OBESE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 1892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 294 ANIMAL NUMBER: C90821 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 695 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 9:45 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SG), 1893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 295 ANIMAL NUMBER: C90822 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 516.1 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: TODD ACKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); DORSAL; WEEK 76; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; TAILPROXIMAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 8 X 5 MM, RED, DARK RED, GRAY, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -INFARCT,-SLIGHT, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL 1894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 296 ANIMAL NUMBER: C90822 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 516.1 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: TODD ACKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -MASS(ES); MID DORSAL LUMBAR REGION: SINGLE MASS, 17 X 15 X 9 MM, TAN AND SOFT; CUT SURFACE EXUDES A TAN, CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED TAIL (TI) : -ABRASION/ULCERATIONS; NEAR BASE OF TAIL: SINGLE BROWN AND MOIST ABRADED AREA, 7 X 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT 1895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 297 ANIMAL NUMBER: C90822 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 516.1 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: TODD ACKER RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 298 ANIMAL NUMBER: C90823 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 760.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:40 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PROSTATE (PR) : -NODULAR; SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 299 ANIMAL NUMBER: C90823 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 760.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:40 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY) 1898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 300 ANIMAL NUMBER: C90824 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 679.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:06 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MASS(ES); PAPILLARY PROCESS: SINGLE MASS, 13 X 18 X 26 MM, FIRM, LOBULATED, RED, DARK RED, TAN AND YELLOW; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (HEPATIC) NOT IDENTIFIED KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -CALCULUS(I); BILATERAL, WITHIN PELVIS: MULTIPLE HARD, TAN AND WHITE CALCULI, UP TO 2 X 2 X 4 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SEVERE, FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, MULTI FOCAL THYROID (TY) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 1899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 301 ANIMAL NUMBER: C90824 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 679.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:06 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 6 X 9 MM, SOFT, LOBULATED, RED, DARK RED AND TAN GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MATERIAL -BLOOD FILM TAKEN -ANIMAL OBESE MOIST PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 1900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 302 ANIMAL NUMBER: C90824 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 679.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:06 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 303 ANIMAL NUMBER: C90825 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 10:10 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 10 X 5 MM; COLLECTED ON ROUTINE SECTIONS GENERAL COMMENT (GC) : -ANIMAL OBESE HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT 1902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 304 ANIMAL NUMBER: C90825 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/11/99 STUDY DAY OF DEATH: 685 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/11/99 10:10 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 305 ANIMAL NUMBER: C90826 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 13:44 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 8 X 6 X 5 MM (LEFT IS 3 X 2 X 1 MM) (CAP 22) HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, FOCAL KIDNEY (KD) : -INFARCT,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL 1904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 306 ANIMAL NUMBER: C90826 SEX: :MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 13:44 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) GENERAL COMMENT (GC) : -ANIMAL OBESE 1905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 307 ANIMAL NUMBER: C90826 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/29/99 STUDY DAY OF DEATH: 642 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/29/99 13:44 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 308 ANIMAL NUMBER: C90827 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/11/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 924.3 GRAMS DATE AND TIME OF NECROPSY: 05/11/99 15:02 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; ORAL; CLEAR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 7 X 5 MM DARK RED, SOFT AND LOBULATED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 309 ANIMAL NUMBER: C90827 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/11/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 924.3 GRAMS DATE AND TIME OF NECROPSY: 05/11/99 15:02 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -CYST(S); BILATERAL, PECTORAL REGIONS: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER (CAP 21) GENERAL COMMENT (GC) : -DISCHARGE-ORAL; CLEAR AND MOIST -BLOOD FILM TAKEN -ANIMAL OBESE MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL 1908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 310 ANIMAL NUMBER: C90827 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/11/99 STUDY DAY OF DEATH: 471 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 924.3 GRAMS DATE AND TIME OF NECROPSY: 05/11/99 15:02 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 311 ANIMAL NUMBER: C90828 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/08/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/08/99 8:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; DILATED PUPIL; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 312 ANIMAL NUMBER: C90828 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/08/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/08/99 8:38 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 313 ANIMAL NUMBER: C90829 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1188.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:39 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 91; 20 X 15MM; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -MASS(ES); NEAR SPLEEN: SINGLE MASS, 6 MM IN DIAMETER, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (SEE PANCREATIC) SKIN (SK) : -ABRASION(S)/ULCERATION(S); RIGHT AXILLARY REGION: SINGLE TAN, RED ABRADED AREA, 17 MM IN DIAMETER (CAP 22) HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -TELANGIECTASIS,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL -B-FIBROMA,-NEOPLASM PRESENT 1912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 314 ANIMAL NUMBER: C90829 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1188.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:39 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -CYST(S); SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER (CAP 27) LN, ANT MES/PANC (AP) : -DIFFUSELY RED; (CAP 24) LN, LUMBAR/ILIAC (LM) : -CYSTS; BILATERAL: MULTIPLE CLEAR FLUID FILLED CYSTS ON EACH, UP TO 7 MM IN DIAMETER (CAP 26) BILE DUCT (BD) : -LARGE; UP TO 2 MM IN DIAMETER (CAP 25) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-MODERATE MULTI-FOCAL LN, ANT MES/PANC (AP) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE FOCAL BILE DUCT (BD) : -DILATED,-PRESENT 1913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 315 ANIMAL NUMBER: C90829 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1188.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:39 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) 1914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 316 ANIMAL NUMBER: C90830 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 783.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 5 X 4 MM; COLLECTED ON ROUTINE SECTIONS LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, FOCAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 1915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 317 ANIMAL NUMBER: C90830 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 783.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY) 1916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 318 ANIMAL NUMBER: C90831 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 573.2 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:27 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0229 .0237 2.2907 2.0209 2.0483 18.1672 1.6127 .8274 1.8061 1.8342 .0125 .0119 .0040 % .0041 % .3996 % .3526 % .3573 % 3.1694 % .2814 % .1443 % .3151 % .3200 % .0022 % .0021 % OBSERVATIONS NECROPSY .0100 .0103 1.0000 .8822 .8942 7.9309 .7040 .3612 .7884 .8007 .0055 .0052 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 1917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 319 ANIMAL NUMBER: C90831 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 573.2 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:27 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 320 ANIMAL NUMBER: C90832 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 854.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT IS 5 MM IN DIAMETER) HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 1919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 321 ANIMAL NUMBER: C90832 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 854.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: TAN AND FIRM LN, MANDIBULAR (MN) : -LARGE; BILATERAL: UP TO 11 X 8 X 4 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN OFF AT BASE -ANIMAL OBESE URINARY BLADDER (UB) : -CALCULUS,-PRESENT PROSTATE (PR) : -MINERALIZATION, LUMINAL,-MODERATELY SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -DILATATION, SINUSOIDAL,-SLIGHT, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 1920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 322 ANIMAL NUMBER: C90832 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 854.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE) 1921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 323 ANIMAL NUMBER: C90833 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 364.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:50 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 11.3215 ------------------------------------- ------------------------------- 3.1077 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 324 ANIMAL NUMBER: C90833 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 364.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:50 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 325 ANIMAL NUMBER: C90834 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 376.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:18 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 11.5017 ------------------------------------- ------------------------------- 3.0533 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 326 ANIMAL NUMBER: C90834 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 376.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:18 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 327 ANIMAL NUMBER: C90835 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 634.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN -CYST(S); BILATERAL, DIFFUSE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 1 MM IN DIAMETER -LARGE; BILATERAL: EACH IS 31 X 23 X 15 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -CYST,-SLIGHT, MULTI-FOCAL -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE PANCREAS (PA) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 328 ANIMAL NUMBER: C90835 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 634.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 1927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 329 ANIMAL NUMBER: C90836 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 513.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:13 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0284 .0274 2.0529 1.8193 1.9550 14.7082 1.5994 .7967 1.6068 1.5707 .0101 .0099 .0055 % .0053 % .3999 % .3544 % .3809 % 2.8654 % .3116 % .1552 % .3130 % .3060 % .0020 % .0019 % OBSERVATIONS NECROPSY .0138 .0133 1.0000 .8862 .9523 7.1646 .7791 .3881 .7827 .7651 .0049 .0048 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MINIMAL, DIFFUSE KIDNEY (KD) : -DEGENERATION, TUBULAR,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 1928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 330 ANIMAL NUMBER: C90836 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 513.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:13 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 331 ANIMAL NUMBER: C90837 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 396.6 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK 61; 20 X 25MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; HEAD MAXILLARY-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED SKIN (SK) : -MASS(ES); RIGHT SIDE OF HEAD: SINGLE MASS, 34 X 26 X 24 MM, TAN, RED, VASCULAR AND LOBULATED; ON SECTION, EXUDES A TAN GRANULAR SEMISOLID; COLLECTED INTACT; OVERLYING SKIN HAS MULTIPLE RED, MOIST ULCERATED AREAS, UP TO 7 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE MANDIBULAR) HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL, MULTI-FOCAL HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL COLON (CO) : -PARASITISM,-PRESENT SKIN (SK) : -EDEMA,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL 1930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 332 ANIMAL NUMBER: C90837 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 396.6 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY URINARY BLADDER (UB) : -THICKENED WALL; DIFFUSE LN, MANDIBULAR (MN) : -LARGE; RIGHT: 7 X 4 X 2 MM (FOR COMPARISON, LEFT IS 4 X 2 X 1 MM) (CAP 21) GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -ANIMAL THIN URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTIFOCAL CAUSE OF DEATH (CD) : -AUDITORY SEBACEOUS GLAND CARCINOMA,PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, DIFFUSE MAMMARY, MALE (MM) : -MINERALIZATION,-MODERATE, FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT AUDITORY SEB GL (AS) : -M-CARCINOMA,-NEOPLASM PRESENT 1931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 333 ANIMAL NUMBER: C90837 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 396.6 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT DISTENDED WITH GAS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 334 ANIMAL NUMBER: C90838 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 900.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:11 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); MOUTH; WEEK 91; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN -CYST(S); LEFT, CORTEX: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER -LARGE; BILATERAL: EACH IS 26 X 20 X 14 MM HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF BOTH VENTRICLES: MULTIPLE GRAY INDISTINCT AREAS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -CYST,-MODERATELY SEVERE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -INFLAMMATION, VASCULAR,-MODERATE, FOCAL -PIGMENT,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL 1933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 335 ANIMAL NUMBER: C90838 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 900.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:11 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 5 X 5 X 4 MM; APPEARS SWOLLEN SKIN (SK) : -RAISED AREA(S); LEFT UPPER LIP: SINGLE RED, TAN, RAISED AREA, 4 X 3 X 2 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THYROID (TY) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 336 ANIMAL NUMBER: C90838 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 900.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:11 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) 1935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 337 ANIMAL NUMBER: C90839 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/19/99 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/19/99 15:03 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; LIMB-HIND-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES KIDNEY (KD) : -LARGE; RIGHT: 31 X 21 X 15 MM (LEFT, SEE MASS COMMENT) -MASS(ES); INVOLVING ENTIRE LEFT: SINGLE MASS, 55 X 50 X 45 MM, RED, DARK RED, GREY, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (RENAL) NORMAL (CAP 23) LUNG (LU) : -UNCOLLAPSED; ALL LOBES HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -NECROSIS,-MODERATELY SEVERE, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE -M-NEPHROBLASTOMA,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL TESTIS (TE) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL LUNG (LU) : -HEMORRHAGE,-SLIGHT, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL 1936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 338 ANIMAL NUMBER: C90839 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/19/99 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/19/99 15:03 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); MID VENTRAL THORACIC REGION: SINGLE DRY, DARK RED CRUSTED AREA, 5 X 4 MM (CAP 22); POSSIBLY CONSISTENT WITH SCAB (CERVICAL REGION) AS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS, EXTENDING TO VENTRAL ABDOMINAL REGION: DRY, BROWN MATERIAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE, DIFFUSE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -INFLAMMATION, ACUTE,-MINIMAL, FOCAL -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -NEPHROBLASTOMA,-PRESENT LN, RENAL (RL) : -DILATATION, SINUSOIDAL,-MINIMAL, MULTI FOCAL 1937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 339 ANIMAL NUMBER: C90839 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/19/99 STUDY DAY OF DEATH: 693 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/19/99 15:03 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 340 ANIMAL NUMBER: C90840 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 810.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:44 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION LN, MESENTERIC (MS) : -LARGE; UP TO 8 MM IN DIAMETER -DIFFUSELY RED; DARK RED HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EROSION,-MODERATELY SEVERE, MULTI FOCAL 1939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 341 ANIMAL NUMBER: C90840 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 810.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:44 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: SINGLE DARK RED AND MOIST CRUSTED AREA ON EACH, RIGHT IS 15 MM IN DIAMETER, LEFT IS 17 X 12 MM; BOTH COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; INVOLVING ENTIRE PERIANAL REGION: BROWN AND MOIST -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN FOOT/FOOTPAD(S) (FP) : -EXOSTOSES,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 1940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 342 ANIMAL NUMBER: C90840 SEX: MALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 810.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:44 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY), URINARY BLADDER (UB) 1941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 343 ANIMAL NUMBER: C90841 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 504.1 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 14:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; DISCOLORED/LIGHT RED; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; LEFT -COLLAPSED GLOBE-ANTE MORTEM; LEFT ADRENAL, CORTEX (AC) : -SMALL; LEFT: 3 X 3 X 2 MM (RIGHT IS 5 X 4 X 3 MM) HISTOPATHOLOGY EYE (EY) : -FIBROSIS, CORNEAL,-MODERATE, MULTI FOCAL -INFLAMMATION, ACUTE, CORNEAL,MODERATE, MULTI-FOCAL -VASCULARIZATION, CORNEAL,-MODERATELY SEVERE, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL PANCREAS (PA) : -INFLAMMATION, VASCULAR,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL 1942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 344 ANIMAL NUMBER: C90841 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 504.1 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 14:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TESTIS (TE) : -SMALL; BILATERAL: BOTH ARE 17 X 10 X 5 MM; BOTH ARE SOFT PITUITARY (PI) : -DIFFUSELY RED SEMINAL VESICLES (SV) : -SMALL; BILATERAL: LEFT IS 15 X 10 X 10 MM, RIGHT IS 15 X 6 X 2 MM -MOTTLED; LEFT: RED, TAN AND BROWN ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, DIFFUSE THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL -MINERALIZATION, LUMINAL,-MINIMAL, FOCAL SEMINAL VESICLES (SV) : ^UNREMARKABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 1943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 345 ANIMAL NUMBER: C90841 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 504.1 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 14:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 22) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE TAN AREA, 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; EXTENDING INTO VENTRAL ABDOMINAL REGION: YELLOW -BLOOD FILM TAKEN -FLUID-THORACIC CAVITY; CLEAR FLUID LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL AORTA (AO) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL 1944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 346 ANIMAL NUMBER: C90841 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 504.1 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 14:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 347 ANIMAL NUMBER: C90842 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 559.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE RED AREA, 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION LN, MESENTERIC (MS) : -MOTTLED; DARK RED AND TAN TESTIS (TE) : -SMALL; LEFT: 18 X 8 X 7 MM (RIGHT IS 25 X 15 X 8 MM) HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATELY SEVERE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE, MULTI-FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 348 ANIMAL NUMBER: C90842 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 559.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:42 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 1947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 349 ANIMAL NUMBER: C90843 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 523.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:09 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0292 .0292 2.2810 1.8280 1.8980 17.8078 1.7571 .7643 1.5660 1.5425 .0126 .0108 .0056 % .0056 % .4356 % .3491 % .3624 % 3.4004 % .3355 % .1459 % .2990 % .2945 % .0024 % .0021 % OBSERVATIONS NECROPSY .0128 .0128 1.0000 .8014 .8321 7.8070 .7703 .3351 .6865 .6762 .0055 .0047 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MINIMAL, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 350 ANIMAL NUMBER: C90843 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 523.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:09 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 351 ANIMAL NUMBER: C90844 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 669.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN PITUITARY (PI) : -LARGE; 6 X 4 X 3 MM STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN AND BROWN ERODED AREA, 8 X 2 MM -THICKENED WALL; DIFFUSE HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT 1950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 352 ANIMAL NUMBER: C90844 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 669.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM JOINT, OTHER (JT) : -LARGE; RIGHT TARSAL JOINT: 17 MM IN DIAMETER (LEFT IS 10 MM IN DIAMETER); COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT JOINT, OTHER (JT) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EXOSTOSES,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL 1951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 353 ANIMAL NUMBER: C90844 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 669.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) 1952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 354 ANIMAL NUMBER: C90845 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 645.2 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:35 PROSECTOR: LYNN HORNER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; GENERALIZED; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN SKIN; PAWS-ALL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 7 X 6 DARK RED, GREY AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM MM, HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL 1953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 355 ANIMAL NUMBER: C90845 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 645.2 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:35 PROSECTOR: LYNN HORNER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERIANAL REGION: MOIST BROWN MATERIAL -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ALL FOUR FEET: DARK BROWN, MOIST MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 356 ANIMAL NUMBER: C90845 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 645.2 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:35 PROSECTOR: LYNN HORNER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1955 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 357 ANIMAL NUMBER: C90846 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 324.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:45 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 9.5115 ------------------------------------- ------------------------------- 2.9338 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1956 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 358 ANIMAL NUMBER: C90846 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 324.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:45 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1957 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 359 ANIMAL NUMBER: C90847 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 389.8 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE, ALONG MARGIN NEAR HILUS: SINGLE TAN AREA, 7 X 3 MM; COLLECTED ON ROUTINE SECTION -DARK FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE BROWN FOCUS, 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -SMALL; LEFT: 4 MM IN DIAMETER (RIGHT IS 6 MM IN DIAMETER) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL 1958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 360 ANIMAL NUMBER: C90847 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 389.8 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 9 X 9 MM, RED, GREY, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 361 ANIMAL NUMBER: C90847 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 389.8 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 362 ANIMAL NUMBER: C90848 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 973.1 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 11:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -VACUOLATION,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 1961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 363 ANIMAL NUMBER: C90848 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 973.1 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 11:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -MALOCCLUSION; BILATERAL: UPPER INCISORS GENERAL INFORMATION (XX) : >NOTE:>RED DISCHARGE (LEFT EYE) NOTED IN CLINICAL OBSERVATIONS WAS NOT SEEN AT NECROPSY 1962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 364 ANIMAL NUMBER: C90848 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 973.1 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 11:22 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 365 ANIMAL NUMBER: C90849 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:20 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 366 ANIMAL NUMBER: C90849 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:20 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR: LEFT IS 9 X 3 X 2 MM, RIGHT IS 13 X 7 X 5 MM (CAP 22) FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE RED, TAN, MOIST, SLIGHTLY RAISED ABRADED AREA, 13 X 10 X 4 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL 1965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 367 ANIMAL NUMBER: C90849 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:20 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (MS), 1966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 368 ANIMAL NUMBER: C90850 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:02 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, PINPOINT AND UP TO 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -ROUGH SURFACE, DIFFUSE; LEFT -SMALL; LEFT: 19 X 10 X 8 MM (RIGHT IS 24 X 15 X 10 MM) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT IS 4 X 3 X 2 MM) HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL KIDNEY (KD) : -CYST,-MODERATE, MULTI-FOCAL -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL THYROID (TY) : -NECROSIS,-MODERATE, FOCAL -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 1967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 369 ANIMAL NUMBER: C90850 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:02 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); MID ANTERIOR ASPECT: SINGLE TAN AREA, 2 MM IN DIAMETER STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 4 X 3 MM PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED AND TAN AREA, 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: RED -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-SLIGHT, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL 1968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 370 ANIMAL NUMBER: C90850 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 579.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:02 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), URINARY BLADDER (UB) 1969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 371 ANIMAL NUMBER: C90851 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:11 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN, SLIGHTLY RAISED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS -DIFFUSELY LIGHT; ALL LOBES: TAN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED FOCI, PINPOINT AND UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE DARK BROWN AREA, 2 X 1 MM URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE TAN AND FIRM CALCULUS, 10 X 5 X 5 MM; COLLECTED INTACT HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -PIGMENT,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : >UNREMARKABLE 1970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 372 ANIMAL NUMBER: C90851 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:11 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 10 X 6 X 6 MM (CAP 22) FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL HINDFEET: SINGLE DARK BROWN ABRADED AREA ON EACH, LEFT IS 9 X 7 X 5 MM, RIGHT IS 5 MM IN DIAMETER; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 1971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 373 ANIMAL NUMBER: C90851 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 792.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:11 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), TESTIS (TE), THYROID (TY) 1972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 374 ANIMAL NUMBER: C90852 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 508.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:17 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0262 .0277 2.1921 1.9487 1.9761 15.8553 1.7581 .6186 1.7403 1.8014 .0134 .0151 .0052 % .0054 % .4311 % .3832 % .3886 % 3.1181 % .3457 % .1217 % .3422 % .3543 % .0026 % .0030 % OBSERVATIONS NECROPSY .0120 .0126 1.0000 .8890 .9015 7.2329 .8020 .2822 .7939 .8218 .0061 .0069 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 375 ANIMAL NUMBER: C90852 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 508.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:17 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 1974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 376 ANIMAL NUMBER: C90853 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 305.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:46 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 9.4927 ------------------------------------- ------------------------------- 3.1113 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 377 ANIMAL NUMBER: C90853 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 305.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:46 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 1976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 378 ANIMAL NUMBER: C90854 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 746.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:02 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATELY SEVERE, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 379 ANIMAL NUMBER: C90854 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 746.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:02 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), URINARY BLADDER (UB) 1978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 380 ANIMAL NUMBER: C90855 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 779.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:32 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL-CERVICALLEFT; WEEK 105; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED SKIN (SK) : -CRUSTED AREA(S); MID DORSAL CERVICAL REGION: SINGLE DRY BROWN CRUSTED AREA, 10 MM IN DIAMETER (CAP 22) HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LN, MESENTERIC (MS) : >UNREMARKABLE LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL 1979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 381 ANIMAL NUMBER: C90855 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 779.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:32 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY) 1980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 382 ANIMAL NUMBER: C90856 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 897.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:39 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT ADRENAL, CORTEX (AC) : -MASS(ES); LEFT: SINGLE MASS, 19 X 15 X 11 MM, RED, DARK RED, TAN, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE RENAL) HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : >UNREMARKABLE ADRENAL, MEDULLA (MA) : -NECROSIS,-MODERATE, MULTI-FOCAL -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 1981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 383 ANIMAL NUMBER: C90856 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 897.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:39 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 1982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 384 ANIMAL NUMBER: C90856 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 897.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:39 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) 1983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 385 ANIMAL NUMBER: C90857 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 452.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL PITUITARY (PI) : -MASS(ES); 14 X 10 X 5 MM, TAN, GRAY, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL TESTIS (TE) : -MINERALIZATION,-MINIMAL, FOCAL SPINAL CORD (SC) : -VACUOLATION, WHITE MATTER,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 1984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 386 ANIMAL NUMBER: C90857 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 452.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 2 MM IN DIAMETER FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND FOOT: SINGLE DARK RED AND MOIST ABRADED AREA, 11 X 10 X 7 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN STOMACH, GL (ST) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATELY SEVERE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 1985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 387 ANIMAL NUMBER: C90857 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 452.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SM), 1986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 388 ANIMAL NUMBER: C90858 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:43 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -MASS(ES); ALONG SPLEEN: SINGLE MASS, 3 MM IN DIAMETER, TAN AND FIRM; COLLECTED INTACT WITH SPLEEN; REGIONAL LN (SEE PANCREATIC) THYROID (TY) : -LARGE; LEFT: 9 X 6 X 4 MM (RIGHT IS 6 X 2 X 1 MM) CECUM (CE) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL -M-CARCINOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT CECUM (CE) : >UNREMARKABLE URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 389 ANIMAL NUMBER: C90858 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:43 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE LN, ANT MES/PANC (AP) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -N-ISLET CELL CARCINOMA,-NEOPLASM PRESENT 1988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 390 ANIMAL NUMBER: C90858 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 581.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:43 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) 1989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 391 ANIMAL NUMBER: C90859 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 538.6 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 15:17 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -SMALL; BILATERAL: EACH IS 15 X 10 X 3 MM; BOTH ARE SOFT PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, BROWN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -EDEMA,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE 1990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 392 ANIMAL NUMBER: C90859 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 538.6 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 15:17 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 5 X 2 MM GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST -BLOOD FILM TAKEN YELLOW AND EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 393 ANIMAL NUMBER: C90859 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 538.6 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 15:17 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 1992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 394 ANIMAL NUMBER: C90860 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND BROWN PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID POSTERIOR ASPECT: SINGLE DARK RED AREA, 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, FOCAL -TELANGIECTASIS,-SLIGHT, FOCAL KIDNEY (KD) : -CYST,-MINIMAL, FOCAL -INFARCT,-MODERATE, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 395 ANIMAL NUMBER: C90860 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:40 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), THYROID (TY), URINARY BLADDER (UB) 1994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 396 ANIMAL NUMBER: C90861 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/04/99 STUDY DAY OF DEATH: 495 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 456.3 GRAMS DATE AND TIME OF NECROPSY: 06/04/99 14:33 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, DARK RED, TAN AND SOFT SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 5 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -STAINS - SKIN/HAIRCOAT; VENTRAL MID ABDOMINAL REGION, HAIRCOAT: RED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, DIFFUSE -B-ADENOMA,-NEOPLASM PRESENT SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 1995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 397 ANIMAL NUMBER: C90861 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/04/99 STUDY DAY OF DEATH: 495 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 456.3 GRAMS DATE AND TIME OF NECROPSY: 06/04/99 14:33 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 398 ANIMAL NUMBER: C90862 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 435.0 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:30 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -MOTTLED; BILATERAL: TAN AND BROWN PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 8 X 12 MM, SOFT, RED, DARK RED AND TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 1997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 399 ANIMAL NUMBER: C90862 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 435.0 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:30 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 1998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 400 ANIMAL NUMBER: C90863 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 643.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:29 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0303 .0309 2.4617 2.3739 2.3341 18.7061 2.3756 .7464 1.7051 1.7163 .0100 .0129 .0047 % .0048 % .3826 % .3690 % .3628 % 2.9074 % .3692 % .1160 % .2650 % .2668 % .0016 % .0020 % OBSERVATIONS NECROPSY .0123 .0126 1.0000 .9643 .9482 7.5989 .9650 .3032 .6927 .6972 .0041 .0052 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 1999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 401 ANIMAL NUMBER: C90863 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 643.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:29 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 402 ANIMAL NUMBER: C90864 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 98; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN PITUITARY (PI) : -RAISED AREA(S); LEFT ANTERIOR ASPECT: SINGLE TAN AREA, 2 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL,-SLIGHT, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT 2001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 403 ANIMAL NUMBER: C90864 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); LEFT LATERAL SHOULDER REGION: SINGLE DARK RED AND TAN, SLIGHTLY RAISED AREA, 7 MM IN DIAMETER (CAP 22) LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED, DARK RED, AND TAN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD, HAIRCOAT: RED -BONE MARROW SMEAR TAKEN -ANIMAL OBESE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 404 ANIMAL NUMBER: C90864 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), URINARY BLADDER (UB) 2003 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 405 ANIMAL NUMBER: C90865 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 701.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:43 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: SINGLE MOIST RED CRUSTED AREA, 9 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATELY SEVERE, MULTI-FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT -DESQUAMATION, TRANSITIONAL EPITHELIUM,MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL 2004 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 406 ANIMAL NUMBER: C90865 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 701.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:43 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), TESTIS (TE) 2005 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 407 ANIMAL NUMBER: C90866 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 359.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:36 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 11.2529 ------------------------------------- ------------------------------- 3.1302 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2006 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 408 ANIMAL NUMBER: C90866 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 359.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:36 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2007 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 409 ANIMAL NUMBER: C90867 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 64; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); VISCERAL SURFACE OF LEFT LATERAL LOBE: SINGLE RED AREA, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION SKIN (SK) : -MASS(ES); DORSAL LUMBAR REGION: SINGLE MASS, 27 X 20 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 22); OVERLYING SKIN OF MASS HAS A SINGLE TAN AND BLACK ULCERATED AREA, 12 MM IN DIAMETER; COLLECTED INTACT WITH MASS HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL SKIN (SK) : -HEMORRHAGE,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL -ULCERATION,-SLIGHT, FOCAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2008 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 410 ANIMAL NUMBER: C90867 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), THYROID (TY), LN, INGUINAL (IN) 2009 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 411 ANIMAL NUMBER: C90868 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 621.7 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HEART (HT) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE PITUITARY (PI) : -MASS(ES); SINGLE MASS, 14 X 8 X 7 MM RED, BROWN, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL HEART (HT) : >TISSUE MISSING PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, FOCAL -FIBROSIS,-SEVERE, MULTI-FOCAL 2010 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 412 ANIMAL NUMBER: C90868 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 621.7 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: SINGLE TAN, RED AND DRY AREA ON EACH, LEFT IS 5 X 3 MM, RIGHT IS 9 X 7 MM; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; LEFT VENTRAL ABDOMINAL REGION: DARK RED AND DRY FOOT/FOOTPAD(S) (FP) : -INFLAMMATION, CHRONIC ACTIVE,MODERATE, MULTI-FOCAL 2011 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 413 ANIMAL NUMBER: C90868 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 621.7 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:42 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2012 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 414 ANIMAL NUMBER: C90869 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 877.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; SCROTUM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -ABRASION(S)/ULCERATION(S); PERINEAL REGION: SINGLE TAN, RED AND MOIST ULCERATED AREA, 8 MM IN DIAMETER (CAP 22) HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, MULTI-FOCAL SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL -B-FIBROMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -EDEMA,-MODERATE, MULTI-FOCAL -HYPERPLASIA, TRANSITIONAL CELL,MODERATE, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2013 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 415 ANIMAL NUMBER: C90869 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 877.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -LARGE; UP TO 15 X 11 X 11 MM; APPEARS CYSTIC (CAP 23) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: UP TO 8 X 5 X 3 MM; APPEARS CYSTIC (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-SEVERE, FOCAL LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL 2014 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 416 ANIMAL NUMBER: C90869 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 877.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY) 2015 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 417 ANIMAL NUMBER: C90870 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 515.6 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:30 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -CONTAINS FLUID; RIGHT: CLEAR FLUID PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 12 MM FIRM, RED, DARK RED, TAN, AND BROWN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY BROWN MATERIAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE >NOTE:>RIGHT TESTIS LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2016 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 418 ANIMAL NUMBER: C90870 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 515.6 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:30 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY YELLOW MATERIAL -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2017 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 419 ANIMAL NUMBER: C90871 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 718.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:22 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -GRANULAR MATERIAL; LEFT, WITHIN PELVIS: GREEN GRANULAR MATERIAL TESTIS (TE) : -SMALL; RIGHT: 15 X 10 X 8 MM (LEFT IS 23 X 14 X 10 MM) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, FOCAL TESTIS (TE) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2018 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 420 ANIMAL NUMBER: C90871 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 718.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:22 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU) 2019 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 421 ANIMAL NUMBER: C90872 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/24/99 6:54 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -SEMIFLUID MATERIAL-PELVIS; RIGHT: LIGHT GREEN, CREAMY SEMIFLUID -CALCULUS(I); BILATERAL: MULTIPLE TAN, HARD CALCULI, UP TO 5 MM IN DIAMETER ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 5 MM IN DIAMETER BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, FOCAL -MINERALIZATION, CORNEAL,-SLIGHT, FOCAL -ULCERATION, CORNEAL,-MODERATE, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NECROSIS, PAPILLARY,-MINIMAL, FOCAL -PYELONEPHRITIS,-MODERATELY SEVERE, DIFFUSE SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE, MULTI FOCAL ADRENAL, CORTEX (AC) : >UNREMARKABLE TESTIS (TE) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL 2020 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 422 ANIMAL NUMBER: C90872 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/24/99 6:54 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED AND SOFT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION,-MODERATE, MULTI-FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATELY SEVERE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PYELONEPHRITIS,-PRESENT 2021 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 423 ANIMAL NUMBER: C90872 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/24/99 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/24/99 6:54 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2022 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 424 ANIMAL NUMBER: C90873 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 622.3 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 8:17 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRALTHORACIC; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 8 X 4 MM, RED, DARK RED, TAN, SOFT AND LOBULATED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2023 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 425 ANIMAL NUMBER: C90873 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 622.3 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 8:17 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT: SINGLE RED AND DRY AREA, 7 X 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL AND THORACIC REGIONS: RED AND DRY HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2024 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 426 ANIMAL NUMBER: C90873 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 622.3 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 8:17 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2025 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 427 ANIMAL NUMBER: C90874 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:14 PROSECTOR: JOHN S. HALFORD RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY STOMACH, GL (ST) : -MASS(ES); MUCOSA, ALONG GREATER CURVATURE: SINGLE TAN AND SOFT MASS, 6 X 5 X 3 MM; ON SECTION EXUDES A WHITE CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (PANCREATIC) NOT IDENTIFIED PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL STOMACH, GL (ST) : -CYST,-PRESENT STOMACH, NONGL (SU) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2026 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 428 ANIMAL NUMBER: C90874 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:14 PROSECTOR: JOHN S. HALFORD RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM (COLLECTED ON ROUTINE SECTION) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -GI TRACT-LARGE LUMEN; JEJUNUM, ILEUM AND CECUM, DIFFUSE: UP TO 25 MM IN DIAMETER CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 2027 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 429 ANIMAL NUMBER: C90874 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:14 PROSECTOR: JOHN S. HALFORD RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2028 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 430 ANIMAL NUMBER: C90875 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 632 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/19/99 9:50 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS HISTOPATHOLOGY PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 2029 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 431 ANIMAL NUMBER: C90875 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/19/99 STUDY DAY OF DEATH: 632 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/19/99 9:50 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2030 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 432 ANIMAL NUMBER: C90876 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 02/15/99 STUDY DAY OF DEATH: 386 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: 888.8 GRAMS DATE AND TIME OF NECROPSY: 02/15/99 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; PARALYSIS; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MUSCLE, SKELETAL (SM) : -DIFFUSELY RED; MUSCLE SURROUNDING VENTRAL SURFACE OF MID THORACIC REGION: DARK RED; COLLECTED INTACT WITH SPINAL CORD HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL LUNG (LU) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT EPIDIDYMIDES (EP) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL 2031 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 433 ANIMAL NUMBER: C90876 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 02/15/99 STUDY DAY OF DEATH: 386 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: 888.8 GRAMS DATE AND TIME OF NECROPSY: 02/15/99 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS CAUSE OF DEATH (CD) : -TRAUMA,-PRESENT 2032 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 434 ANIMAL NUMBER: C90876 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 02/15/99 STUDY DAY OF DEATH: 386 STUDY WEEK OF DEATH: 56 TERMINAL BODY WEIGHT: 888.8 GRAMS DATE AND TIME OF NECROPSY: 02/15/99 13:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2033 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 435 ANIMAL NUMBER: C90877 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:32 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0294 .0220 2.2403 1.8338 1.7847 17.3893 1.9130 .5725 1.6055 1.6214 .0136 .0150 .0065 % .0048 % .4925 % .4031 % .3923 % 3.8227 % .4205 % .1259 % .3529 % .3564 % .0030 % .0033 % OBSERVATIONS NECROPSY .0131 .0098 1.0000 .8186 .7966 7.7620 .8539 .2555 .7166 .7237 .0061 .0067 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2034 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 436 ANIMAL NUMBER: C90877 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 454.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:32 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS) 2035 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 437 ANIMAL NUMBER: C90878 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/99 STUDY DAY OF DEATH: 369 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/99 8:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) -AUTOLYSIS-SEVERE HISTOPATHOLOGY PANCREAS (PA) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 2036 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 438 ANIMAL NUMBER: C90878 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/29/99 STUDY DAY OF DEATH: 369 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/29/99 8:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2037 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 439 ANIMAL NUMBER: C90879 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 443.5 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:12 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES, DIFFUSE: RED AND BROWN KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 8 X 8 MM, FIRM, TAN, RED AND DARK RED RECTUM (RE) : >NOTE:>TRIMMING COMMENT: NOT FOUND AT TRIMMING HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >TISSUE MISSING RECTUM (RE) : >TISSUE MISSING 2038 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 440 ANIMAL NUMBER: C90879 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 443.5 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:12 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN MOIST BROWN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2039 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 441 ANIMAL NUMBER: C90879 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 443.5 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:12 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2040 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 442 ANIMAL NUMBER: C90880 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 566.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0324 .0265 2.2340 2.4180 2.3287 20.5702 2.1495 .8744 1.6470 1.6603 .0165 .0152 .0057 % .0047 % .3946 % .4271 % .4114 % 3.6337 % .3797 % .1545 % .2909 % .2933 % .0029 % .0027 % OBSERVATIONS NECROPSY .0145 .0119 1.0000 1.0824 1.0424 9.2078 .9622 .3914 .7372 .7432 .0074 .0068 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2041 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 443 ANIMAL NUMBER: C90880 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 566.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE) 2042 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 444 ANIMAL NUMBER: C90881 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 978.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:43 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 5 X 4 MM (LEFT IS 4 X 3 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SEVERE, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : >UNREMARKABLE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL 2043 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 445 ANIMAL NUMBER: C90881 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 978.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:43 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, DARK RED TAN, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2044 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 446 ANIMAL NUMBER: C90881 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 978.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:43 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), URINARY BLADDER (UB) 2045 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 447 ANIMAL NUMBER: C90882 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 985.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -CYST(S); MID ANTERIOR ASPECT: SINGLE CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2046 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 448 ANIMAL NUMBER: C90882 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 985.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 2047 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 449 ANIMAL NUMBER: C90883 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 621.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:29 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 X 5 X 5 MM, DARK RED, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN, TAN, AND FIRM LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 22) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2048 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 450 ANIMAL NUMBER: C90883 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 621.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:29 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) 2049 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 451 ANIMAL NUMBER: C90884 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, RED, GRAY, TAN, AND FIRM FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL HINDFEET: SINGLE RED, TAN AND MOIST AREA ON EACH, LEFT IS 11 X 10 MM, RIGHT IS 3 MM IN DIAMETER; BOTH COLLECTED; TISSUE SURROUNDING LEFT APPEARS SWOLLEN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2050 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 452 ANIMAL NUMBER: C90884 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 2051 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 453 ANIMAL NUMBER: C90885 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -ADHESION(S); LEFT LOBE ADHERED TO PLEURA: MULTIPLE GRAY AND FIBROUS ADHESIONS; COLLECTED INTACT THYROID (TY) : -LARGE; RIGHT: 8 X 6 X 6 MM (LEFT IS 4 X 3 X 2 MM) LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 12 X 7 X 5 MM (RIGHT IS 7 X 3 X 3 MM) -DIFFUSELY RED; LEFT: DARK RED (CAP 22) HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2052 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 454 ANIMAL NUMBER: C90885 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 751.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DARK RED AND TAN ABRADED AREA, 10 X 8 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE) 2053 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 455 ANIMAL NUMBER: C90886 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 11:38 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); PAW-HIND-LEFT; WEEK 89; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 11 MM IN DIAMETER, RED, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2054 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 456 ANIMAL NUMBER: C90886 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 11:38 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TAIL (TI) : -CRUSTED AREA(S); DIFFUSE ALONG ENTIRE LENGTH: MULTIPLE DARK BROWN AND SLIGHTLY RAISED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HIND FOOT: SINGLE BROWN, RED, AND MOIST ABRADED AREA, 12 MM IN DIAMETER; COLLECTED LN, POPLITEAL (PP) : -LARGE; RIGHT: UP TO 11 X 10 X 6 MM (LEFT IS NOT IDENTIFIED) (CAP 22) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; COVERING ENTIRE ABDOMINAL REGION: DRY BROWN MATERIAL -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL LN, POPLITEAL (PP) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL 2055 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 457 ANIMAL NUMBER: C90886 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 470.7 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 11:38 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2056 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 458 ANIMAL NUMBER: C90887 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 701.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:47 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRALCERVICAL; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 9 MM, SOFT, RED AND DARK RED STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN AND BROWN ERODED AREAS, UP TO 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, MULTI-FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL 2057 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 459 ANIMAL NUMBER: C90887 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 701.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:47 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND HEAD: DRY RED MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2058 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 460 ANIMAL NUMBER: C90887 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 701.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:47 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2059 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 461 ANIMAL NUMBER: C90888 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 433.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:46 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); DORSAL; WEEK 79; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: TAN AND BROWN PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 12 MM, FIRM, RED, DARK RED AND TAN STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE DARK BROWN ERODED AREA, 2 X 3 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MINIMAL, FOCAL -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL 2060 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 462 ANIMAL NUMBER: C90888 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 433.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:46 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE DARK BROWN ERODED AREA, 2 X 4 MM SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC REGION: SINGLE DRY, TAN AREA, 5 X 6 MM (CAP 21) PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: MULTIPLE TAN AND GREEN NODULES GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK BROWN MOIST MATERIAL -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN STOMACH, NONGL (SU) : -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2061 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 463 ANIMAL NUMBER: C90888 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 433.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 14:46 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2062 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 464 ANIMAL NUMBER: C90889 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 818.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY THYROID (TY) : -LARGE; RIGHT: 9 X 6 X 6 MM (LEFT IS 5 X 3 X 3 MM) SKIN (SK) : -MASS(ES); LEFT LATERAL HINDLEG: SINGLE MASS, 15 X 10 X 5 MM, TAN AND SOFT; ON SECTION EXUDES A TAN CASEOUS SEMISOLID MATERIAL (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED -CRUSTED AREA(S); LEFT LATERAL LUMBAR REGION: SINGLE DRY DARK RED AREA, 5 MM IN DIAMETER (CAP 22) PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT -B-KERATOACANTHOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2063 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 465 ANIMAL NUMBER: C90889 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 818.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DARK RED ABRADED AREA, 10 X 8 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-SLIGHT, FOCAL -FIBROSIS,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU) 2064 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 466 ANIMAL NUMBER: C90890 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 360.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:45 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 11.2612 ------------------------------------- ------------------------------- 3.1212 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2065 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 467 ANIMAL NUMBER: C90890 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 360.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:45 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2066 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 468 ANIMAL NUMBER: C90891 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:45 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 13.2084 ------------------------------------- ------------------------------- 3.5449 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2067 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 469 ANIMAL NUMBER: C90891 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:45 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2068 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 470 ANIMAL NUMBER: C90892 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 419.6 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYROID (TY) : >NOTE:>TRIMMING COMMENT: ONE MISSING FROM WET TISSUE PARATHYROID (PT) : >NOTE:>TRIMMING COMMENT: ONE MISSING FROM WET TISSUE PITUITARY (PI) : -MASS(ES); 5 X 7 X 9 MM SOFT, DARK RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LUNG (LU) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -ONE EXAMINED,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2069 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 471 ANIMAL NUMBER: C90892 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 419.6 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -CYST(S); BILATERAL, CERVICAL REGIONS: MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED -ANIMAL THIN MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 2070 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 472 ANIMAL NUMBER: C90892 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 419.6 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2071 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 473 ANIMAL NUMBER: C90893 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 908.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED, TAN, AND BROWN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK RED AREA, 3 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATELY SEVERE, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2072 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 474 ANIMAL NUMBER: C90893 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 908.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL OBESE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 2073 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 475 ANIMAL NUMBER: C90894 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 655.4 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 14:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY THYROID (TY) : -RED FOCUS(I)/AREA(S); RIGHT: SINGLE DARK RED AREA, 2 MM IN DIAMETER STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, GL (ST) : -EDEMA,-MINIMAL, FOCAL -EROSION,-MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL PROSTATE (PR) : -MINERALIZATION, LUMINAL,-MODERATE, MULTI-FOCAL 2074 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 476 ANIMAL NUMBER: C90894 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 655.4 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 14:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); WITHIN MESENTERY, ADJACENT TO COLON: SINGLE MASS, 35 X 25 X 20 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A CLEAR RED FLUID; COLLECTED INTACT; REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST BROWN MATERIAL -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL CERVICAL REGION: DRY DARK RED MATERIAL EPIDIDYMIDES (EP) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -CARCINOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS ON THYMUS SECTION CAVITY, ABDOM (PC) : -M-CARCINOMA,-NEOPLASM PRESENT 2075 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 477 ANIMAL NUMBER: C90894 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 684 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 655.4 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 14:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (SE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2076 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 478 ANIMAL NUMBER: C90895 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 657 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:35 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES -MOTTLED; ALL LOBES: RED, DARK RED, TAN AND YELLOW LUNG (LU) : -MOTTLED; ALL LOBES: RED, DARK RED AND TAN -UNCOLLAPSED; ALL LOBES HISTOPATHOLOGY LIVER (LI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-SEVERE, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC 2077 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 479 ANIMAL NUMBER: C90895 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 657 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:35 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DRY, DARK RED MATERIAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT 2078 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 480 ANIMAL NUMBER: C90895 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/13/99 STUDY DAY OF DEATH: 657 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/13/99 7:35 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) 2079 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 481 ANIMAL NUMBER: C90896 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 489.3 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:54 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 10 X 7 MM, DARK RED, RED, TAN AND SOFT SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 20 X 10 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -TELANGIECTASIS,-MODERATE, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 2080 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 482 ANIMAL NUMBER: C90896 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 489.3 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:54 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2081 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 483 ANIMAL NUMBER: C90897 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/17/99 STUDY DAY OF DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/17/99 7:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED FOCI, PINPOINT AND UP TO 2 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2082 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 484 ANIMAL NUMBER: C90897 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/17/99 STUDY DAY OF DEATH: 661 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/17/99 7:30 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2083 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 485 ANIMAL NUMBER: C90898 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/01/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 475.4 GRAMS DATE AND TIME OF NECROPSY: 04/01/99 10:09 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 9 MM, SOFT, RED, TAN AND BROWN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL 2084 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 486 ANIMAL NUMBER: C90898 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/01/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 475.4 GRAMS DATE AND TIME OF NECROPSY: 04/01/99 10:09 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2085 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 487 ANIMAL NUMBER: C90898 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/01/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 475.4 GRAMS DATE AND TIME OF NECROPSY: 04/01/99 10:09 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2086 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 488 ANIMAL NUMBER: C90899 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 478.0 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 8:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MASS(ES); NEAR HILUS OF PAPILLARY PROCESS: SINGLE MASS, 21 X 16 X 10 MM, RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (HEPATIC) NOT IDENTIFIED PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, DARK RED, TAN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2087 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 489 ANIMAL NUMBER: C90899 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 478.0 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 8:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 2088 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 490 ANIMAL NUMBER: C90899 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 478.0 GRAMS DATE AND TIME OF NECROPSY: 01/17/00 8:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2089 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 491 ANIMAL NUMBER: C90900 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:01 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS TESTIS (TE) : -LARGE; RIGHT: 26 X 18 X 15 MM (LEFT IS 20 X 15 X 13 MM) -RED/YELLOW FOCUS(I)/AREA(S); RIGHT, BENEATH TUNICA: DIFFUSE HISTOPATHOLOGY LIVER (LI) : -FIBROSIS,-MINIMAL, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -PIGMENT,-SLIGHT, FOCAL KIDNEY (KD) : -CYST,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE, MULTI FOCAL -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE, FOCAL 2090 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 492 ANIMAL NUMBER: C90900 SEX: MALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 655.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:01 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) 2091 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-212 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e V I o f X II Page 2092 of 4873 Covance 6329-212 3M T-6316.1 VOLUME VI OF XII CONTENTS Page A P P E N D I X 6 ( C o n t i n u e d ) .................................................................................................................................................... 2 0 9 4 I n d i v i d u a l A n a t o m i c P a t h o l o g y D a t a .................................................................................................................... 2 0 9 5 2093 APPENDIX 6 (Continued) In d iv id u a l A n a to m ic P a th o lo g y D a ta Covance 6329-212 3M T-6316.1 2094 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 493 ANIMAL NUMBER: C90901 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/21/99 8:14 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 10 X 8 MM, RED, TAN, DARK RED AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2095 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 494 ANIMAL NUMBER: C90901 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/21/99 8:14 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN LINEAR AREAS, UP TO 3 X 2 MM STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL -PIGMENT,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -MINERALIZATION, LUMINAL,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2096 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 495 ANIMAL NUMBER: C90901 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/21/99 8:14 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2097 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 496 ANIMAL NUMBER: C90902 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 625.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:34 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0261 .0207 2.4596 2.0836 2.0882 25.2925 1.9060 .7737 1.6258 1.7143 .0259 .0204 .0042 % .0033 % .3932 % .3331 % .3338 % 4.0429 % .3047 % .1237 % .2599 % .2740 % .0041 % .0033 % OBSERVATIONS NECROPSY .0106 .0084 1.0000 .8471 .8490 10.2832 .7749 .3146 .6610 .6970 .0105 .0083 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2098 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 497 ANIMAL NUMBER: C90902 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 625.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:34 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2099 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 498 ANIMAL NUMBER: C90903 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 12:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -FIBROSIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2100 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 499 ANIMAL NUMBER: C90903 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 12:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK FOCI, PINPOINT AND UP TO 1 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-NASAL; RED AND MOIST -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: MOIST AND YELLOW PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL 2101 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 500 ANIMAL NUMBER: C90903 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 703 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 12:55 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2102 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 501 ANIMAL NUMBER: C90904 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MASS(ES); ATTACHED TO VISCERAL SURFACE OF RIGHT MEDIAN LOBE: SINGLE MASS, 17 X 15 X 6 MM, TAN, RED, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NOT IDENTIFIED HISTOPATHOLOGY LIVER (LI) : -PIGMENT,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2103 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 502 ANIMAL NUMBER: C90904 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -MOTTLED; RIGHT: RED AND TAN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-SLIGHT, FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE 2104 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 503 ANIMAL NUMBER: C90904 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:55 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2105 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 504 ANIMAL NUMBER: C90905 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 420.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:32 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0327 .0281 2.1911 1.9594 1.9588 21.7331 1.6862 .6461 1.5387 1.4719 .0141 .0111 .0078 % .0067 % .5206 % .4655 % .4654 % 5.1635 % .4006 % .1535 % .3656 % .3497 % .0033 % .0026 % OBSERVATIONS NECROPSY .0149 .0128 1.0000 .8943 .8940 9.9188 .7696 .2949 .7023 .6718 .0064 .0051 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2106 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 505 ANIMAL NUMBER: C90905 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 420.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:32 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2107 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 506 ANIMAL NUMBER: C90906 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/08/00 STUDY DAY OF DEATH: 713 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 578.4 GRAMS DATE AND TIME OF NECROPSY: 01/08/00 8:09 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -MOTTLED; BILATERAL: DARK RED AND TAN PITUITARY (PI) : -MASS(ES); 9 X 8 X 4 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2108 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 507 ANIMAL NUMBER: C90906 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/08/00 STUDY DAY OF DEATH: 713 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 578.4 GRAMS DATE AND TIME OF NECROPSY: 01/08/00 8:09 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: BROWN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -HYPERPLASIA, EPITHELIAL,-MODERATE, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 2109 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 508 ANIMAL NUMBER: C90906 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/08/00 STUDY DAY OF DEATH: 713 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 578.4 GRAMS DATE AND TIME OF NECROPSY: 01/08/00 8:09 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2110 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 509 ANIMAL NUMBER: C90907 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 449.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0302 .0300 2.1970 1.9941 1.9800 21.0493 1.7525 .6630 1.7456 1.8060 .0156 .0121 .0067 % .0067 % .4890 % .4438 % .4407 % 4.6849 % .3901 % .1476 % .3885 % .4020 % .0035 % .0027 % OBSERVATIONS NECROPSY .0137 .0137 1.0000 .9076 .9012 9.5809 .7977 .3018 .7945 .8220 .0071 .0055 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2111 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 510 ANIMAL NUMBER: C90907 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 449.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2112 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 511 ANIMAL NUMBER: C90908 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 786.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:49 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT TESTIS (TE) : -RED FOCUS(I)/AREA(S); LEFT, BENEATH TUNICA: SINGLE DARK RED AREA, 7 X 6 MM HISTOPATHOLOGY BRAIN (BR) : -B-GRANULAR CELL TUMOR,-NEOPLASM PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT TESTIS (TE) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2113 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 512 ANIMAL NUMBER: C90908 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 786.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:49 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); RIGHT POSTERIOR ASPECT: SINGLE TAN AREA, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2114 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 513 ANIMAL NUMBER: C90908 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 786.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:49 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2115 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 514 ANIMAL NUMBER: C90909 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 790.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:45 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0337 .0281 2.4329 3.0718 3.1441 37.4539 2.7025 1.2044 1.8463 1.8493 .0277 .0393 .0043 % .0036 % .3078 % .3887 % .3978 % 4.7392 % .3420 % .1524 % .2336 % .2340 % .0035 % .0050 % OBSERVATIONS NECROPSY .0139 .0116 1.0000 1.2626 1.2923 15.3948 1.1108 .4950 .7589 .7601 .0114 .0162 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -FIBROSIS,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL 2116 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 515 ANIMAL NUMBER: C90909 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 790.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:45 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HYPERPLASIA,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2117 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 516 ANIMAL NUMBER: C90909 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 790.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:45 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2118 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 517 ANIMAL NUMBER: C90910 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATELY SEVERE, FOCAL ADRENAL, CORTEX (AC) : -HYPERPLASIA, CORTICAL,-SLIGHT, FOCAL -VACUOLATION,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2119 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 518 ANIMAL NUMBER: C90910 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -LIGHT FOCUS(I)/AREA(S); MID ASPECT: SINGLE GRAY AREA, 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2120 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 519 ANIMAL NUMBER: C90910 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:46 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2121 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 520 ANIMAL NUMBER: C90911 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); MOUTH; WEEK 95; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: SINGLE TAN AREA, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -LARGE; ALL LOBES -DARK FOCUS(I)/AREA(S); LEFT LATERAL LOBE: SINGLE DARK BROWN AREA, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN ADRENAL, CORTEX (AC) : -DIFFUSELY DARK; BILATERAL: DARK BROWN HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, MULTI FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, DIFFUSE -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : >UNREMARKABLE PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION,-SLIGHT, FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL 2122 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 521 ANIMAL NUMBER: C90911 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -RAISED AREA(S); LEFT UPPER LIP: SINGLE GRAY, RAISED AREA, 3 MM IN DIAMETER (CAP 22); POSSIBLY CONSISTENT WITH WART-LIKE LESION (MOUTH) AS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THYROID (TY) : -CYST, FOLLICULAR,-MODERATELY SEVERE, FOCAL -HYPERPLASIA, FOLLICULAR CELL,MODERATE, FOCAL RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2123 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 522 ANIMAL NUMBER: C90911 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2124 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 523 ANIMAL NUMBER: C90912 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 568 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 403.4 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 13:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -SMALL; BILATERAL: EACH IS 4 X 3 X 2 MM TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 10 X 5 MM; BOTH ARE SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : >UNREMARKABLE TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL 2125 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 524 ANIMAL NUMBER: C90912 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 568 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 403.4 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 13:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, TAN AND SOFT PROSTATE (PR) : -SMALL; 10 X 8 X 5 MM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 6 X 3 MM TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE TAIL: MULTIPLE DRY AND BROWN CRUSTED AREAS, UP TO 5 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL -MINERALIZATION, LUMINAL,-MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2126 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 525 ANIMAL NUMBER: C90912 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 568 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 403.4 GRAMS DATE AND TIME OF NECROPSY: 08/16/99 13:25 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SG), 2127 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 526 ANIMAL NUMBER: C90913 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 607.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:46 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0320 .0270 2.2108 2.0030 1.9603 30.9958 2.0592 .7228 1.6724 1.7264 .0256 .0249 .0053 % .0044 % .3637 % .3295 % .3225 % 5.0988 % .3387 % .1189 % .2751 % .2840 % .0042 % .0041 % OBSERVATIONS NECROPSY .0145 .0122 1.0000 .9060 .8867 14.0202 .9314 .3269 .7565 .7809 .0116 .0113 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HYPERPLASIA,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2128 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 527 ANIMAL NUMBER: C90913 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 607.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:46 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; UPPER RIGHT INCISOR -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; UPPER LEFT INCISOR MISSING 2129 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 528 ANIMAL NUMBER: C90913 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 607.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:46 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2130 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 529 ANIMAL NUMBER: C90914 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 655.2 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 14:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); LIMB-HIND-LEFT; WEEK 76; 65 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2131 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 530 ANIMAL NUMBER: C90914 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 655.2 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 14:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PARATHYROID (PT) : -LARGE; RIGHT: 5 MM IN DIAMETER (LEFT IS 1 MM IN DIAMETER) PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, DARK RED, RED, TAN, AND SOFT SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 20 X 12 MM MAMMARY, MALE (MM) : -MASS(ES); LEFT LATERAL LUMBAR REGION: SINGLE MASS, 20 X 20 X 10 MM, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE PARATHYROID (PT) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : >UNREMARKABLE >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS LIPOMA 2132 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 531 ANIMAL NUMBER: C90914 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 655.2 GRAMS DATE AND TIME OF NECROPSY: 12/22/99 14:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2133 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 532 ANIMAL NUMBER: C90915 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 13:03 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >AUTOLYTIC STOMACH, NONGL (SU) : >AUTOLYTIC DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC 2134 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 533 ANIMAL NUMBER: C90915 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 13:03 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DARK RED AND DRY -AUTOLYSIS-SEVERE; ENTIRE ANIMAL PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 2135 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 534 ANIMAL NUMBER: C90915 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 13:03 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2136 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 535 ANIMAL NUMBER: C90916 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES -MOTTLED; ALL LOBES: RED AND TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, FOLLICULAR CELL,-SLIGHT, FOCAL RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2137 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 536 ANIMAL NUMBER: C90916 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 723.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2138 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 537 ANIMAL NUMBER: C90917 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 495.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0301 .0250 2.2389 2.2821 2.2453 24.6177 1.5560 .7012 1.8522 1.8916 .0135 .0120 .0061 % .0050 % .4518 % .4606 % .4531 % 4.9683 % .3140 % .1415 % .3738 % .3818 % .0027 % .0024 % OBSERVATIONS NECROPSY .0134 .0112 1.0000 1.0193 1.0029 10.9954 .6950 .3132 .8273 .8449 .0060 .0054 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2139 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 538 ANIMAL NUMBER: C90917 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 495.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2140 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 539 ANIMAL NUMBER: C90918 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:32 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0390 .0325 2.4945 2.4369 2.5132 29.5214 1.8496 .6504 1.0050 1.6530 .0274 .0263 .0058 % .0049 % .3724 % .3638 % .3752 % 4.4068 % .2761 % .0971 % .1500 % .2468 % .0041 % .0039 % OBSERVATIONS NECROPSY .0156 .0130 1.0000 .9769 1.0075 11.8346 .7415 .2607 .4029 .6627 .0110 .0105 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2141 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 540 ANIMAL NUMBER: C90918 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:32 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 7 X 5 MM, RED, DARK RED AND TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2142 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 541 ANIMAL NUMBER: C90918 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 669.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:32 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2143 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 542 ANIMAL NUMBER: C90919 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/30/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/30/99 10:00 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAWS-FRONT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; EARS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); LEFT LUNG (LU) : -MOTTLED; ALL LOBES: RED AND DARK RED -ADHESION(S); ALL RIGHT LOBES TO EACH OTHER: MULTIPLE GREY FIBROUS ADHESIONS; COLLECTED INTACT URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; DARK RED GRANULAR SEMIFLUID -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,MODERATE, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC URINARY BLADDER (UB) : >UNREMARKABLE 2144 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 543 ANIMAL NUMBER: C90919 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/30/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/30/99 10:00 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PROSTATE (PR) : -DIFFUSELY RED; DARK RED SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: GREY AND CLEAR -LARGE; BILATERAL: EACH IS 30 X 15 X 5 MM -DIFFUSELY RED; BILATERAL: DARK RED JOINT, OTHER (JT) : -LARGE; LEFT CARPAL JOINT: UP TO 10 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; 30 X 20 X 15 MM GENERAL INFORMATION (XX) : >NOTE:>SWOLLEN (RIGHT FRONT PAW) AS NOTED IN CLINICAL OBSERVATIONS WAS NOT OBSERVED AT TIME OF NECROPSY PROSTATE (PR) : -EDEMA,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT JOINT, OTHER (JT) : -EXOSTOSES,-SEVERE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL 2145 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 544 ANIMAL NUMBER: C90919 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/30/99 STUDY DAY OF DEATH: 551 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/30/99 10:00 PROSECTOR: NANCY DIEDRICH RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2146 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 545 ANIMAL NUMBER: C90920 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 643.5 GRAMS DATE AND TIME OF NECROPSY: 01/22/00 9:36 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; SCROTUM; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -PYELONEPHRITIS,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -HYPERTROPHY, CORTICAL CELL,-SLIGHT, FOCAL -THROMBUS,-SLIGHT, FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL 2147 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 546 ANIMAL NUMBER: C90920 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 643.5 GRAMS DATE AND TIME OF NECROPSY: 01/22/00 9:36 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, RED, GRAY AND FIRM GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2148 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 547 ANIMAL NUMBER: C90920 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/22/00 STUDY DAY OF DEATH: 727 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 643.5 GRAMS DATE AND TIME OF NECROPSY: 01/22/00 9:36 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2149 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 548 ANIMAL NUMBER: C90921 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:24 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0248 .0270 2.2400 2.5569 2.6329 29.9661 1.6740 .6825 1.8368 1.8132 .0142 .0149 .0048 % .0052 % .4302 % .4911 % .5056 % 5.7550 % .3215 % .1311 % .3528 % .3482 % .0027 % .0029 % OBSERVATIONS NECROPSY .0111 .0121 1.0000 1.1415 1.1754 13.3777 .7473 .3047 .8200 .8095 .0063 .0067 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED SKIN (SK) : -RED FOCUS(I)/AREA(S); OVERLYING RIGHT CARPAL JOINT: SINGLE DARK RED AREA, 5 MM IN DIAMETER (CAP 21) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2150 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 549 ANIMAL NUMBER: C90921 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 520.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:24 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2151 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 550 ANIMAL NUMBER: C90922 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 315.7 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:32 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, DARK RED, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT, MULTI FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2152 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 551 ANIMAL NUMBER: C90922 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 315.7 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:32 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 15 X 5 MM GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOWISHBROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 2153 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 552 ANIMAL NUMBER: C90922 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 315.7 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:32 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2154 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 553 ANIMAL NUMBER: C90923 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/18/99 STUDY DAY OF DEATH: 601 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 465.7 GRAMS DATE AND TIME OF NECROPSY: 09/18/99 12:56 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADCRANIAL; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, DARK RED, BLACK, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2155 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 554 ANIMAL NUMBER: C90923 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/18/99 STUDY DAY OF DEATH: 601 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 465.7 GRAMS DATE AND TIME OF NECROPSY: 09/18/99 12:56 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 5 X 2 MM LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: LEFT IS 15 X 8 X 3 MM, RIGHT IS 13 X 5 X 3 MM; BOTH APPEAR GELATINOUS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; BILATERAL: RED -STAINS - SKIN/HAIRCOAT; VENTRAL PERIANAL, PERINEUM, AND ABDOMINAL REGIONS: DARK RED -TAIL - BENT/MISSING; 46 MM FROM TIP, BENT: CORE OF TAIL IS EXPOSED; SURROUNDING TISSUE IS DARK RED; COLLECTED SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL TAIL (TI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2156 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 555 ANIMAL NUMBER: C90923 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/18/99 STUDY DAY OF DEATH: 601 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 465.7 GRAMS DATE AND TIME OF NECROPSY: 09/18/99 12:56 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SM), 2157 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 556 ANIMAL NUMBER: C90924 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 614.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:21 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0402 .0332 2.4172 2.8755 2.8181 33.4640 2.1269 .8329 1.8639 1.8872 .0199 .0288 .0065 % .0054 % .3932 % .4678 % .4585 % 5.4440 % .3460 % .1355 % .3032 % .3070 % .0032 % .0047 % OBSERVATIONS NECROPSY .0166 .0137 1.0000 1.1896 1.1659 13.8441 .8799 .3446 .7711 .7807 .0082 .0119 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2158 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 557 ANIMAL NUMBER: C90924 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 614.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:21 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2159 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 558 ANIMAL NUMBER: C90925 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: CHANESTA ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES, PINPOINT AND UP TO 1 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM: DARK RED, GRAY, LOBULATED, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-SLIGHT, FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2160 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 559 ANIMAL NUMBER: C90925 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: CHANESTA ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, TISSUE SURROUNDING PYLORIC VALVE: MULTIPLE DARK BROWN FOCI, UP TO 1 MM IN DIAMETER URINARY BLADDER (UB) : -SEMIFLUID MATERIAL; WITHIN LUMEN: DARK BROWN MUCOID SEMIFLUID MATERIAL PROSTATE (PR) : -NODULAR; PROXIMAL, TISSUE SURROUNDING URETHRA: GREEN AND FIRM MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST AND DRY YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -ANIMAL OBESE STOMACH, GL (ST) : -EDEMA,-MINIMAL, FOCAL -EROSION,-MINIMAL, FOCAL URINARY BLADDER (UB) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : >UNREMARKABLE 2161 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 560 ANIMAL NUMBER: C90925 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/02/99 STUDY DAY OF DEATH: 646 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 624.5 GRAMS DATE AND TIME OF NECROPSY: 11/02/99 14:45 PROSECTOR: JOHN S. HALFORD RECORDER: CHANESTA ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2162 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 561 ANIMAL NUMBER: C90926 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:59 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES, DIFFUSE: BROWN, YELLOW AND DARK RED TESTIS (TE) : -SMALL; BILATERAL: EACH IS 22 X 12 X 8 MM; BOTH APPEAR SOFT PITUITARY (PI) : -MASS(ES); 10 X 9 X 7 MM, TAN, RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2163 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 562 ANIMAL NUMBER: C90926 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:59 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK RED MATERIAL -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : ^UNREMARKABLE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2164 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 563 ANIMAL NUMBER: C90926 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 432.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:59 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2165 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 564 ANIMAL NUMBER: C90927 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 762.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 95; 70 X 65MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -MOTTLED; DARK RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2166 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 565 ANIMAL NUMBER: C90927 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 762.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 5 MM IN DIAMETER, RED, DARK RED, GRAY AND SOFT MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 70 X 50 X 40 MM, TAN, SOFT AND ADIPOSE TISSUE-LIKE; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,SLIGHT, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, FOCAL >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS LIPOMA 2167 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 566 ANIMAL NUMBER: C90927 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 762.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2168 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 567 ANIMAL NUMBER: C90928 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:21 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2169 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 568 ANIMAL NUMBER: C90928 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 491.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:21 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), LIVER, OIL RED O (LI1), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2170 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 569 ANIMAL NUMBER: C90929 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:18 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 10 X 8 MM LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED -MASS(ES); ALL LOBES: MULTIPLE MASSES, UP TO 25 X 20 X 15 MM, DARK RED, TAN, AND SOFT; ON SECTION EXUDED DARK RED FLUID AND DARK RED AND TAN SEMISOLID MATERIAL; COLLECTED INTACT; REGIONAL LN (SEE TRACHEOBRONCHIAL) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL TESTIS (TE) : >UNREMARKABLE LUNG (LU) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -MALIGNANT FIBROUS HISTIOSARCOMA,PRESENT HEMATO NEOPLASIA (HN) : -M-MALIGNANT FIBROUS HISTIOCYTOMA,NEOPLASM PRESENT 2171 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 570 ANIMAL NUMBER: C90929 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:18 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY ADIPOSE TISSUE (AT) : -THICKENED; ALONG VENTRAL MID LINE: RED, TAN, AND FRIABLE (CAP 22) LN, TRACHEOBRON (TB) : -DIFFUSELY RED; DARK RED (CAP 21) GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DARK RED AND MOIST -MALOCCLUSION; BILATERAL UPPER INCISORS -FLUID-THORACIC CAVITY; DARK RED SEMIFLUID ADIPOSE TISSUE (AT) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, TRACHEOBRON (TB) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2172 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 571 ANIMAL NUMBER: C90929 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/17/99 13:18 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) (UB), 2173 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 572 ANIMAL NUMBER: C90930 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 587.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:32 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN URINARY BLADDER (UB) : -THICKENED WALL; DIFFUSE -CALCULUS(I); WITHIN LUMEN: MULTIPLE TAN HARD CALCULI, UP TO 8 X 2 X 2 MM -DIFFUSELY RED; MUCOSA: DARK RED PROSTATE (PR) : -LARGE; 28 X 25 X 18 MM -GELATINOUS; DIFFUSE: TAN AND CLEAR HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT -HYPERPLASIA, TRANSITIONAL CELL,MODERATE, DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -EDEMA,-MINIMAL, MULTI-FOCAL 2174 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 573 ANIMAL NUMBER: C90930 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 587.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:32 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR: LEFT IS 10 X 7 X 4 MM, RIGHT IS 15 X 6 X 5 MM (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK YELLOW AND MOIST -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN AT BASE SEMINAL VESICLES (SV) : -FIBROSIS,-SEVERE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL 2175 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 574 ANIMAL NUMBER: C90930 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 587.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:32 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2176 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 575 ANIMAL NUMBER: C90931 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 664.5 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:29 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; LIMITED USE; PAWS-FRONT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY SPLEEN (SP) : -CYST(S); ALONG MARGIN AT CAUDAL END: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER PANCREAS (PA) : -MASS(ES); ADJACENT TO SPLEEN: SINGLE MASS, 7 X 5 X 5 MM, TAN, RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE PANCREATIC) HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -CYST, SEROSAL,-PRESENT PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 2177 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 576 ANIMAL NUMBER: C90931 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 664.5 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:29 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT LN, ANT MES/PANC (AP) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL 2178 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 577 ANIMAL NUMBER: C90931 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 664.5 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 14:29 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2179 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 578 ANIMAL NUMBER: C90932 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST : DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0318 .0352 2.2771 2.3637 2.3739 23.8968 1.8995 .6502 1.9335 1.8941 .0197 .0205 .0057 % .0064 % .4114 % .4270 % .4289 % 4.3174 % .3432 % .1175 % .3493 % .3422 % .0036 % .0037 % OBSERVATIONS NECROPSY .0140 .0155 1.0000 1.0380 1.0425 10.4944 .8342 .2855 .8491 .8318 .0087 .0090 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 2180 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 579 ANIMAL NUMBER: C90932 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2181 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 580 ANIMAL NUMBER: C90932 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 553.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: DONIA MEYER RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2182 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 581 ANIMAL NUMBER: C90933 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 795.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 88; 120 X 110MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES; MARGINS APPEAR ROUNDED LN, MESENTERIC (MS) : -LARGE; UP TO 12 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, MULTI FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -HEMORRHAGE,-MINIMAL, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SKIN (SK) : -B-LIPOMA,-NEOPLASM PRESENT 2183 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 582 ANIMAL NUMBER: C90933 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 795.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 115 X 80 X 45 MM, RED, TAN, VASCULAR AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS LIPOMA 2184 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 583 ANIMAL NUMBER: C90933 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 795.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX) 2185 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 584 ANIMAL NUMBER: C90934 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 15:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 102; 25 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; ORAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS AND CAP 22 KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATELY SEVERE, MULTI FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LUNG (LU) : -HEMORRHAGE,-SLIGHT, FOCAL 2186 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 585 ANIMAL NUMBER: C90934 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 15:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, DARK RED TAN, AND SOFT LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT URINARY BLADDER (UB) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : -NECROSIS,-SLIGHT, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2187 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 586 ANIMAL NUMBER: C90934 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 15:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PROSTATE (PR) : -MASS(ES); INVOLVING ENTIRE GLAND AND ADHERED TO SEMINAL VESICLES, URINARY BLADDER, AND DORSAL WALL OF ABDOMINAL CAVITY: SINGLE MASS, 55 X 40 X 40 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NOT IDENTIFIED MAMMARY, MALE (MM) : -MASS(ES); VENTRAL ABDOMINAL REGION: SINGLE MASS, 70 X 40 X 30 MM, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK RED AND MOIST -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: BROWN AND MOIST -ANIMAL THIN SEMINAL VESICLES (SV) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT MAMMARY, MALE (MM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2188 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 587 ANIMAL NUMBER: C90934 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 533.8 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 15:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), EPIDIDYMIDES (EP) 2189 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 588 ANIMAL NUMBER: C90935 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 12.8689 ------------------------------------- ------------------------------- 3.4790 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2190 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 589 ANIMAL NUMBER: C90935 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 369.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2191 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 590 ANIMAL NUMBER: C90936 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 594.5 GRAMS DATE AND TIME OF NECROPSY: 12/31/99 13:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; DISCOLORED URINE; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -CYST(S); ALONG MARGIN OF RIGHT MEDIAN LOBE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 8 X 6 X 5 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -B-CHOLANGIOMA,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2192 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 591 ANIMAL NUMBER: C90936 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 594.5 GRAMS DATE AND TIME OF NECROPSY: 12/31/99 13:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK RED AND SOFT URINARY BLADDER (UB) : -THICKENED WALL; DIFFUSE -CALCULUS(I); WITHIN LUMEN: MULTIPLE TAN AND HARD CALCULI, UP TO 14 MM IN DIAMETER LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 9 X 7 X 5 MM (CAP 22) TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATELY SEVERE, DIFFUSE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL LN, RENAL (RL) : -DILATATION, SINUSOIDAL,-SEVERE, FOCAL 2193 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 592 ANIMAL NUMBER: C90936 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 594.5 GRAMS DATE AND TIME OF NECROPSY: 12/31/99 13:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, RENAL (RL) : -CYST(S); INVOLVING ENTIRE RIGHT: SINGLE CLEAR FLUID FILLED CYST, 22 MM IN DIAMETER (LEFT NOT IDENTIFIED) (CAP 23) FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL HINDFEET: SINGLE DARK RED, TAN AND MOIST ABRADED AREA ON EACH, EACH IS 7 MM IN DIAMETER; BOTH COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; RED AND MOIST -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN OFF AT BASE -URINARY BLADDER-LARGE LUMEN; 32 X 20 X 20 MM LN, RENAL (RL) : -FIBROSIS,-MODERATE, FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2194 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 593 ANIMAL NUMBER: C90936 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 594.5 GRAMS DATE AND TIME OF NECROPSY: 12/31/99 13:44 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2195 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 594 ANIMAL NUMBER: C90937 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 398.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL TESTIS (TE) : -SMALL; BILATERAL: EACH IS 15 X 10 X 6 MM; BOTH ARE SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATELY SEVERE, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL THYMUS (TH) : -CYST,-PRESENT 2196 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 595 ANIMAL NUMBER: C90937 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 398.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 10 X 10 MM, DARK RED, TAN AND SOFT SEMINAL VESICLES (SV) : -SMALL; RIGHT: 15 X 9 X 3 MM (LEFT IS 21 X 15 X 5 MM) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -MALOCCLUSION; BILATERAL: UPPER INCISORS HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT 2197 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 596 ANIMAL NUMBER: C90937 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 398.5 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2198 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 597 ANIMAL NUMBER: C90938 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/21/99 STUDY DAY OF DEATH: 481 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 488.7 GRAMS DATE AND TIME OF NECROPSY: 05/21/99 14:48 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN SKIN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; HEAD MAXILLARY-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; HEAD MAXILLARY-RIGHT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES: THICKENED -DIFFUSELY DARK; ALL LOBES: DARK BROWN KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -SEMIFLUID MATERIAL-PELVIS; GREENISH BROWN SEMIFLUID RIGHT: PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, BROWN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2199 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 598 ANIMAL NUMBER: C90938 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/21/99 STUDY DAY OF DEATH: 481 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 488.7 GRAMS DATE AND TIME OF NECROPSY: 05/21/99 14:48 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE RED AND TAN ERODED AREAS, UP TO 4 X 2 MM -THICKENED WALL; MUCOSA: DIFFUSE PROSTATE (PR) : -LARGE; 25 X 18 X 8 MM -NODULAR; PROXIMAL, SURROUNDING URETHRA: DARK GREEN AND FIRM GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK BROWN AND DRY -STAINS-PERINEUM/PERIANAL; DARK BROWN AND MOIST -BLOOD FILM TAKEN -DISCHARGE-NASAL; DARK BROWN AND DRY STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -ULCERATION,-MODERATE, FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL -ULCERATION,-MODERATE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2200 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 599 ANIMAL NUMBER: C90938 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/21/99 STUDY DAY OF DEATH: 481 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 488.7 GRAMS DATE AND TIME OF NECROPSY: 05/21/99 14:48 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (SV), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2201 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 600 ANIMAL NUMBER: C90939 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:22 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 12.4132 ------------------------------------- ------------------------------- 3.9245 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2202 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 601 ANIMAL NUMBER: C90939 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:22 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2203 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 602 ANIMAL NUMBER: C90940 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 486.3 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:47 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, DARK RED, GRAY, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN PINPOINT FOCI STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA, DIFFUSE HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -INFILTRATION, FAT, INTERSTITIAL,MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -INFLAMMATION, ACUTE,-MINIMAL, FOCAL STOMACH, NONGL (SU) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL 2204 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 603 ANIMAL NUMBER: C90940 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 486.3 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:47 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND HEAD: DARK RED AND DRY MATERIAL -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 2205 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 604 ANIMAL NUMBER: C90940 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 486.3 GRAMS DATE AND TIME OF NECROPSY: 04/12/99 15:47 PROSECTOR: NANCY DIEDRICH RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2206 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 605 ANIMAL NUMBER: C90941 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 449.0 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 9:56 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 10 X 10 MM, RED, DARK RED, TAN, GRAY, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL 2207 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 606 ANIMAL NUMBER: C90941 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 449.0 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 9:56 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE BROWN ULCERATED AREA, 10 X 5 MM SEMINAL VESICLES (SV) : -LARGE; RIGHT: 35 X 20 X 7 MM (LEFT IS 25 X 14 X 5 MM) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -ANIMAL THIN STOMACH, GL (ST) : -EDEMA,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL -ULCERATION,-SEVERE, FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 2208 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 607 ANIMAL NUMBER: C90941 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 449.0 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 9:56 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2209 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 608 ANIMAL NUMBER: C90942 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 329.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:22 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 11.6629 ------------------------------------- ------------------------------- 3.5417 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2210 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 609 ANIMAL NUMBER: C90942 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 329.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:22 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2211 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 610 ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -MASS(ES); ATTACHED TO RIGHT: SINGLE MASS, 35 X 29 X 16 MM, RED, GRAY, TAN, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (RENAL) NORMAL (CAP 24) PANCREAS (PA) : -MASS(ES); ATTACHED TO DUODENUM: SINGLE MASS, 20 X 18 X 6 MM, RED, GRAY, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (PANCREATIC) NOT IDENTIFIED HISTOPATHOLOGY EYE (EY) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ONE EXAMINED,-PRESENT 2212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 611 ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -MISSING/NOT IDENTIFIED; RIGHT: DUE TO MASS, ATTACHED TO RIGHT KIDNEY; ADRENAL NOT IDENTIFIED LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED TESTIS (TE) : -DIFFUSELY RED; RIGHT THYMUS (TH) : -MASS(ES); INVOLVING ENTIRE ORGAN: SINGLE MASS, 16 X 13 X 11 MM, RED, DARK RED, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); LEFT LOBE: MULTIPLE TAN FOCI, UP TO 1 MM IN DIAMETER -ADHESION(S); ALL LOBES TO PLEURA AND DIAPHRAGM: MULTIPLE GRAY, TAN, AND FIBROUS ADHESIONS; COLLECTED INTACT ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT LN, MESENTERIC (MS) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL TESTIS (TE) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -NECROSIS,-SEVERE, DIFFUSE THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2213 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 612 ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LUNG (LU) : -MASS(ES); INVOLVING ALL RIGHT LOBES: SINGLE MASS, 41 X 23 X 18 MM, RED, DARK RED, GRAY, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE TRACHEOBRONCHIAL) HEART (HT) : -LARGE; RIGHT ATRIUM: 15 X 9 X 6 MM (LEFT IS 5 X 4 X 3 MM) -MOTTLED; EPICARDIUM, DIFFUSE: DARK RED AND GRAY PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, TAN AND SOFT STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA, TISSUE SURROUNDING PYLORIC VALVE: MULTIPLE RED AREAS, UP TO 2 X 1 MM -THICKENED WALL; MUCOSA STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN AND RED ERODED AREAS, UP TO 3 X 2 MM PROSTATE (PR) : -SMALL; 9 X 6 X 3 MM HEART (HT) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PITUITARY (PI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL STOMACH, NONGL (SU) : >UNREMARKABLE SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : >UNREMARKABLE 2214 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 613 ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 8 X 3 MM LN, MEDIASTINAL (ML) : -LARGE; UP TO 8 X 5 X 4 MM (CAP 23) -DIFFUSELY RED; DARK RED LN, TRACHEOBRON (TB) : -DIFFUSELY RED; DARK RED -LARGE; UP TO 12 X 8 X 5 MM (CAP 22) GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY, RED, DARK RED AND YELLOW MATERIAL -ANIMAL THIN SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT LN, RENAL (RL) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LN, TRACHEOBRON (TB) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2215 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 614 ANIMAL NUMBER: C90943 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/26/99 13:20 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP) 2216 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 615 ANIMAL NUMBER: C90944 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 626.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:06 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); BOTH MEDIAN LOBES: MULTIPLE TAN AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTION -DARK FOCUS(I)/AREA(S); VISCERAL SURFACE OF LEFT LATERAL LOBE: SINGLE DARK BROWN AREA, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED PINPOINT FOCI HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ADRENAL TISSUE, ECTOPIC,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2217 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 616 ANIMAL NUMBER: C90944 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 626.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:06 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2218 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 617 ANIMAL NUMBER: C90945 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/30/98 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 549.0 GRAMS DATE AND TIME OF NECROPSY: 11/30/98 10:14 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY THYMUS (TH) : -DIFFUSELY RED; DARK RED STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DRY DARK RED MATERIAL -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY DARK RED MATERIAL HISTOPATHOLOGY THYMUS (TH) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 2219 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 618 ANIMAL NUMBER: C90945 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/30/98 STUDY DAY OF DEATH: 309 STUDY WEEK OF DEATH: 45 TERMINAL BODY WEIGHT: 549.0 GRAMS DATE AND TIME OF NECROPSY: 11/30/98 10:14 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2220 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 619 ANIMAL NUMBER: C90946 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:48 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >HOUSING,FOOD,WATER & MAINT.; TEETH CUT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED, DRY MATERIAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -CYST,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MINIMAL, FOCAL 2221 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 620 ANIMAL NUMBER: C90946 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:48 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: DISTENDED WITH GAS 2222 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 621 ANIMAL NUMBER: C90946 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/19/99 11:48 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2223 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 622 ANIMAL NUMBER: C90947 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:30 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSALTHORACIC-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MASS(ES); RIGHT MEDIAN LOBE: SINGLE MASS, 20 X 15 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NORMAL (CAP 22) THYROID (TY) : -LARGE; BILATERAL: EACH IS 8 X 6 X 4 MM HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2224 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 623 ANIMAL NUMBER: C90947 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:30 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 6 X 4 X 4 MM; APPEARS SWOLLEN STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER SKIN (SK) : -MASS(ES); RIGHT DORSAL THORACIC REGION: SINGLE MASS, 8 MM IN DIAMETER, TAN, FIRM AND FRIABLE; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : ^UNREMARKABLE SKIN (SK) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL -MINERALIZATION, LUMINAL,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, OTHER (LN) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL 2225 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 624 ANIMAL NUMBER: C90947 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:30 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, OTHER (LN) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>HEPATIC LYMPH NODE 2226 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 625 ANIMAL NUMBER: C90947 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 501.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:30 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SG), 2227 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 626 ANIMAL NUMBER: C90948 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 606.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED, TAN, AND BROWN -MASS(ES); RIGHT LATERAL LOBE: SINGLE MASS, 27 X 18 X 13 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (HEPATIC) NOT IDENTIFIED -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -RAISED AREA(S); LEFT POSTERIOR ASPECT: SINGLE TAN AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 X 2 MM RECTUM (RE) : >NOTE:>TRIMMING COMMENT: RECTUM NOT FOUND AT TRIMMING HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, GL (ST) : >UNREMARKABLE RECTUM (RE) : >TISSUE MISSING 2228 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 627 ANIMAL NUMBER: C90948 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 606.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: SINGLE DARK RED AND TAN AREA ON EACH, BOTH ARE 10 MM IN DIAMETER; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED -MALOCCLUSION; BILATERAL: UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE PROSTATE (PR) : -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL -MINERALIZATION, LUMINAL,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, MULTI FOCAL -EXOSTOSES,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, CHRONIC ACTIVE,MODERATE, MULTI-FOCAL 2229 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 628 ANIMAL NUMBER: C90948 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 606.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2230 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 629 ANIMAL NUMBER: C90949 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/21/98 STUDY DAY OF DEATH: 86 STUDY WEEK OF DEATH: 13 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/21/98 14:57 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; SCROTUM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY SPLEEN (SP) : -SMALL; 28 X 7 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; PERIANAL REGION: DRY BROWN STAINS -ANIMAL THIN HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL SPLEEN (SP) : >UNREMARKABLE THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 2231 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 630 ANIMAL NUMBER: C90949 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/21/98 STUDY DAY OF DEATH: 86 STUDY WEEK OF DEATH: 13 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/21/98 14:57 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (TE), 2232 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 631 ANIMAL NUMBER: C90950 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 700.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0261 .0247 2.5063 2.6943 2.7028 37.5350 2.7288 1.6852 1.4975 1.4889 .0262 .0272 .0037 % .0035 % .3577 % .3846 % .3858 % 5.3576 % .3895 % .2405 % .2137 % .2125 % .0037 % .0039 % OBSERVATIONS NECROPSY .0104 .0099 1.0000 1.0750 1.0784 14.9763 1.0888 .6724 .5975 .5941 .0105 .0109 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO BROWN AREAS, EACH IS 2 X 1 MM LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -STASIS, BILE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE, FOCAL STOMACH, GL (ST) : -EROSION,-MINIMAL, MULTI-FOCAL 2233 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 632 ANIMAL NUMBER: C90950 SEX: :MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 700.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN COLON (CO) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2234 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 633 ANIMAL NUMBER: C90950 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 700.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (SE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2235 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 634 ANIMAL NUMBER: C90951 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 613.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0277 .0538 2.3563 2.3550 2.4057 36.9152 2.0966 .9610 1.9872 1.9500 .0254 .0242 .0045 % .0088 % .3839 % .3837 % .3920 % 6.0152 % .3416 % .1566 % .3238 % .3177 % .0041 % .0039 % OBSERVATIONS NECROPSY .0118 .0228 1.0000 .9994 1.0210 15.6666 .8898 .4078 .8434 .8276 .0108 .0103 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT 2236 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 635 ANIMAL NUMBER: C90951 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 613.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR, MISSING; RIGHT UPPER INCISOR, BROKEN AT BASE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2237 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 636 ANIMAL NUMBER: C90951 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 613.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:34 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2238 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 637 ANIMAL NUMBER: C90952 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/99 STUDY DAY OF DEATH: 411 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 581.8 GRAMS DATE AND TIME OF NECROPSY: 03/12/99 7:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL 2239 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 638 ANIMAL NUMBER: C90952 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/99 STUDY DAY OF DEATH: 411 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 581.8 GRAMS DATE AND TIME OF NECROPSY: 03/12/99 7:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2240 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 639 ANIMAL NUMBER: C90952 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/99 STUDY DAY OF DEATH: 411 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 581.8 GRAMS DATE AND TIME OF NECROPSY: 03/12/99 7:41 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2241 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 640 ANIMAL NUMBER: C90953 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:52 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) -MOTTLED; ALL LOBES: DARK RED AND TAN TESTIS (TE) : -SMALL; BILATERAL: EACH IS 16 X 14 X 4 MM; BOTH ARE SOFT THYROID (TY) : -LARGE; BILATERAL: LEFT IS 7 X 5 X 5 MM, RIGHT IS 7 X 3 X 3 MM PARATHYROID (PT) : -LARGE; LEFT: 2 MM IN DIAMETER (RIGHT IS LESS THAN 1 MM IN DIAMETER) PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK BROWN PINPOINT FOCI HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, MULTI FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE THYROID (TY) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2242 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 641 ANIMAL NUMBER: C90953 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:52 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); RIGHT CERVICAL REGION: SINGLE MASS, 20 MM IN DIAMETER, RED, DARK RED, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -EDEMA,-MINIMAL, FOCAL -EROSION,-MINIMAL, FOCAL EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, DIFFUSE MAMMARY, MALE (MM) : -B-ADENOMA,-NEOPLASM PRESENT 2243 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 642 ANIMAL NUMBER: C90953 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 727.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:52 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 2244 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 643 ANIMAL NUMBER: C90954 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 430.4 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 10:26 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); JUNCTION OF MEDIAN LOBES: MULTIPLE TAN AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); 13 X 9 X 8 MM, RED, GREY, TAN, LOBULATED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL HEART (HT) : -MINERALIZATION,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2245 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 644 ANIMAL NUMBER: C90954 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/19/99 STUDY DAY OF DEATH: 479 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 430.4 GRAMS DATE AND TIME OF NECROPSY: 05/19/99 10:26 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: DRY, YELLOW MATERIAL -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN,MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2246 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 645 ANIMAL NUMBER: C90955 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; TAIL-PROXIMAL VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL 2247 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 646 ANIMAL NUMBER: C90955 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TAIL (TI) : -LARGE; PROXIMAL ONE-HALF OF TAIL: UP TO 19 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : -EXOSTOSES,-MODERATE, MULTI-FOCAL 2248 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 647 ANIMAL NUMBER: C90955 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 765.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:45 PROSECTOR: MICHELE MARGGI RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK) , URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2249 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 648 ANIMAL NUMBER: C90956 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:25 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN TESTIS (TE) : -SMALL; BILATERAL: EACH IS 16 X 8 X 7 MM; BOTH APPEAR SOFT HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SEVERE, MULTI-FOCAL KIDNEY (KD) : >UNREMARKABLE TESTIS (TE) : -MINERALIZATION,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2250 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 649 ANIMAL NUMBER: C90956 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:25 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 MM GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; EXTENDING INTO VENTRAL ABDOMINAL REGION: MOIST BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED MATERIAL -MALOCCLUSION; LEFT UPPER INCISOR -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, GL (ST) : -EDEMA,-MODERATELY SEVERE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2251 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 650 ANIMAL NUMBER: C90956 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:25 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN OFF AT BASE RIGHT UPPER INCISOR: MISSING THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2252 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 651 ANIMAL NUMBER: C90957 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0384 .0317 2.2324 2.2615 2.1128 30.0667 2.3390 .6621 1.8336 1.8700 .0171 .0196 .0070 % .0058 % .4062 % .4115 % .3844 % 5.4707 % .4256 % .1205 % .3336 % .3402 % .0031 % .0036 % OBSERVATIONS NECROPSY .0172 .0142 1.0000 1.0130 .9464 13.4683 1.0478 .2966 .8214 .8377 .0077 .0088 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -HYPERPLASIA, MEDIAL, ARTERIAL/ARTERIOLAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2253 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 652 ANIMAL NUMBER: C90957 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -MALOCCLUSION; RIGHT UPPER INCISOR -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; MISSING: LEFT UPPER INCISOR LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2254 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 653 ANIMAL NUMBER: C90957 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 549.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:35 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2255 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 654 ANIMAL NUMBER: C90958 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/12/99 7:41 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED >HOUSING,FOOD,WATER & MAINT.; TEETH CUT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES, UP TO 1 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI FOCAL -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2256 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 655 ANIMAL NUMBER: C90958 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/12/99 7:41 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: SINGLE DRY, DARK RED CRUSTED AREA ON EACH, RIGHT IS 12 X 12 MM, LEFT IS 10 X 6 MM; TISSUE SURROUNDING LEFT APPEARS SWOLLEN; BOTH COLLECTED GENERAL COMMENT (GC) : -MALOCCLUSION; UPPER LEFT INCISOR -TEETH MISSING/BROKEN/WORN; UPPER RIGHT INCISOR: MISSING COLON (CO) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, MULTI FOCAL -EXOSTOSES,-SEVERE, MULTI-FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2257 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 656 ANIMAL NUMBER: C90958 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/12/99 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/12/99 7:41 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2258 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 657 ANIMAL NUMBER: C90959 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 654.7 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:49 PROSECTOR: JAMES T. CALLEN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 11 X 5 X 5 MM, RED, DARK RED, TAN, LOBULATED AND FIRM SALIV GL, MANDIB (SG) : >NOTE:>TRIMMING COMMENT: NOT FOUND WITH WET TISSUE HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL SPLEEN (SP) : -B-HEMANGIOMA,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : >TISSUE MISSING 2259 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 658 ANIMAL NUMBER: C90959 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 654.7 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:49 PROSECTOR: JAMES T. CALLEN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY AND BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY AND RED MATERIAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2260 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 659 ANIMAL NUMBER: C90959 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 654.7 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:49 PROSECTOR: JAMES T. CALLEN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2261 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 660 ANIMAL NUMBER: C90960 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 480.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0336 .0328 2.0634 1.9514 2.0451 24.0869 1.4765 .6268 1.6023 1.6563 .0145 .0125 .0070 % .0068 % .4296 % .4063 % .4258 % 5.0150 % .3074 % .1305 % .3336 % .3448 % .0030 % .0026 % OBSERVATIONS NECROPSY .0163 .0159 1.0000 .9457 .9911 11.6734 .7156 .3038 .7765 .8027 .0070 .0061 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2262 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 661 ANIMAL NUMBER: C90960 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 480.3 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:31 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2263 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 662 ANIMAL NUMBER: C90961 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 309.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:06 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 10.9577 ------------------------------------- ------------------------------- 3.5359 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2264 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 663 ANIMAL NUMBER: C90961 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 309.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:06 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2265 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 664 ANIMAL NUMBER: C90962 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/01/99 STUDY DAY OF DEATH: 492 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 475.5 GRAMS DATE AND TIME OF NECROPSY: 06/01/99 9:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, DARK RED, GREY AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; SINGLE ULCERATED AREA, 4 MM IN DIAMETER; ADHERED TO OUTLYING ADIPOSE TISSUE PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, FOCAL -EROSION,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2266 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 665 ANIMAL NUMBER: C90962 SEX: :MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/01/99 STUDY DAY OF DEATH: 492 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 475.5 GRAMS DATE AND TIME OF NECROPSY: 06/01/99 9:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; ENTIRE VENTRAL SURFACE: YELLOW AND BROWN -BLOOD FILM TAKEN -DISCHARGE-NASAL; BILATERAL: RED 2267 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 666 ANIMAL NUMBER: C90962 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/01/99 STUDY DAY OF DEATH: 492 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 475.5 GRAMS DATE AND TIME OF NECROPSY: 06/01/99 9:11 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2268 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 667 ANIMAL NUMBER: C90963 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 412.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 15:25 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: BROWN PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK RED, TAN AND FIRM HARDERIAN GLAND (HG) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : >TISSUE MISSING STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL 2269 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 668 ANIMAL NUMBER: C90963 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 412.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 15:25 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN, HARD AND SLIGHTLY RAISED AREAS, UP TO 3 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN STOMACH, NONGL (SU) : -EROSION,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2270 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 669 ANIMAL NUMBER: C90963 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 412.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 15:25 PROSECTOR: NOT AVAILABLE RECORDER: NOT AVAILABLE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: NOT AVAILABLE WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2271 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 670 ANIMAL NUMBER: C90964 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/24/99 STUDY DAY OF DEATH: 515 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.0 GRAMS DATE AND TIME OF NECROPSY: 06/24/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >EYES; OPAQUE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EYES; DISCOLORED/LIGHT RED; EYE-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; RIGHT KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, MULTI-FOCAL -MINERALIZATION, CORNEAL,-MINIMAL, MULTI-FOCAL LIVER (LI) : -CYST,-SLIGHT, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT 2272 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 671 ANIMAL NUMBER: C90964 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/24/99 STUDY DAY OF DEATH: 515 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.0 GRAMS DATE AND TIME OF NECROPSY: 06/24/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -THICKENED WALL LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR: EACH IS 5 X 5 X 9 MM (CAP 21) BONE, FEMUR (FE) : -OSTEODYSTROPHY, FIBROUS,-MODERATE, MULTI-FOCAL NERVE, SCIATIC (SN) : >TISSUE MISSING PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE, DIFFUSE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION,-MODERATE, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL MAMMARY, MALE (MM) : -PIGMENT,-SLIGHT, MULTI-FOCAL 2273 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 672 ANIMAL NUMBER: C90964 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/24/99 STUDY DAY OF DEATH: 515 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.0 GRAMS DATE AND TIME OF NECROPSY: 06/24/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, HINDFEET: SINGLE MOIST, RED AND TAN ABRADED AREA ON EACH, LEFT IS 8 X 11 MM, RIGHT IS 13 X 19 MM; SURROUNDING TISSUE APPEARS SWOLLEN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -YELLOWISH MUCOUS MEMBRANES; DIFFUSE: PALE FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL 2274 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 673 ANIMAL NUMBER: C90964 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/24/99 STUDY DAY OF DEATH: 515 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 489.0 GRAMS DATE AND TIME OF NECROPSY: 06/24/99 15:14 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), PANCREAS (PA), LN, MESENTERIC (MS), TESTIS (TE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2275 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 674 ANIMAL NUMBER: C90965 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 533.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:27 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE GRAY AREAS, UP TO 10 X 9 MM HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATELY SEVERE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : >UNREMARKABLE PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -VACUOLATION,-SLIGHT, FOCAL LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL 2276 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 675 ANIMAL NUMBER: C90965 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 533.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:27 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DARK RED, TAN, DRY CRUSTED AREA, 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL 2277 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 676 ANIMAL NUMBER: C90965 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 533.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:27 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2278 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 677 ANIMAL NUMBER: C90966 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 393.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:53 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 13.2827 ------------------------------------- ------------------------------- 3.3730 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2279 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 678 ANIMAL NUMBER: C90966 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 393.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:53 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2280 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 679 ANIMAL NUMBER: C90967 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0400 .0375 2.4167 2.4323 2.3103 23.8000 2.4213 1.0494 1.7744 1.7720 .0205 .0188 .0060 % .0056 % .3639 % .3663 % .3479 % 3.5838 % .3646 % .1580 % .2672 % .2668 % .0031 % .0028 % OBSERVATIONS NECROPSY .0166 .0155 1.0000 1.0065 .9560 9.8481 1.0019 .4342 .7342 .7332 .0085 .0078 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS UNVERIFIED AT NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL 2281 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 680 ANIMAL NUMBER: C90967 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2282 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 681 ANIMAL NUMBER: C90967 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2283 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 682 ANIMAL NUMBER: C90968 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 13 X 8 MM; BOTH ARE SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE -MINERALIZATION,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2284 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 683 ANIMAL NUMBER: C90968 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 601.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (JE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (TH), (TY), (UB), 2285 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 684 ANIMAL NUMBER: C90969 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 9:42 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -LARGE; 88 X 21 X 19 MM; MARGINS APPEAR ROUNDED PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 6 X 4 MM, RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: SINGLE DARK RED ERODED AREA, 3 MM IN DIAMETER FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED AND DRY CRUSTED AREA, 6 MM IN DIAMETER; COLLECTED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SEVERE, DIFFUSE PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2286 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 685 ANIMAL NUMBER: C90969 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 9:42 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2287 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 686 ANIMAL NUMBER: C90970 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 639.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 13:46 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 56; 20 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 8 X 8 X 7 MM, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL -EROSION,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT 2288 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 687 ANIMAL NUMBER: C90970 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 639.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 13:46 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -MASS(ES); MID DORSAL THORACIC REGION: SINGLE MASS, 40 X 35 X 35 MM, TAN, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DARK RED CRUSTED AREA, 20 MM IN DIAMETER; COLLECTED INTACT FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND FOOT: SINGLE DARK RED AND MOIST ABRADED AREA, 13 X 10 X 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -ANIMAL OBESE PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATELY SEVERE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2289 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 688 ANIMAL NUMBER: C90970 SEX: MALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 639.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 13:46 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (MS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2290 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 689 ANIMAL NUMBER: C90971 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 262.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:17 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0288 .0200 1.7426 1.1749 1.2073 20.2788 1.0138 .4768 1.6178 1.5791 .0112 .0093 .0110 % .0076 % .6651 % .4484 % .4608 % 7.7400 % .3869 % .1820 % .6175 % .6027 % .0043 % .0035 % OBSERVATIONS NECROPSY .0165 .0115 1.0000 .6742 .6928 11.6371 .5818 .2736 .9284 .9062 .0064 .0053 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 14 X 2 MM; COLLECTED ON ROUTINE SECTION -LARGE; ALL LOBES -DEPRESSED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT AND RIGHT MEDIAN LOBES: SINGLE, LINEAR, DEPRESSED AREA ON EACH, LEFT IS 15 X 2 MM, RIGHT IS 20 X 2 MM; COLLECTED ON ROUTINE SECTION AND EXTRA SECTION COLLECTED LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL 2291 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 690 ANIMAL NUMBER: C90971 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 262.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:17 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: TERRI L. WILLIAMS LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : ^UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), DUODENUM (DU) 2292 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 691 ANIMAL NUMBER: C90972 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 301.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:50 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0184 .0222 2.0763 1.2294 1.3130 18.5147 1.0329 .4625 1.8046 1.7682 .0091 .0076 .0061 % .0074 % .6891 % .4080 % .4358 % 6.1449 % .3428 % .1535 % .5989 % .5869 % .0030 % .0025 % OBSERVATIONS NECROPSY .0089 .0107 1.0000 .5921 .6324 8.9172 .4975 .2228 .8691 .8516 .0044 .0037 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -LARGE; ALL LOBES -DIFFUSELY DARK; ALL LOBES: DARK BROWN STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO DARK BROWN FOCI, EACH IS 1 MM IN DIAMETER CECUM (CE) : -DIFFUSELY RED; DIFFUSE LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CECUM (CE) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2293 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 692 ANIMAL NUMBER: C90972 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 301.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:50 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2294 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 693 ANIMAL NUMBER: C90984 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 328.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:24 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 16.3999 ------------------------------------- ------------------------------- 4.9939 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2295 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 694 ANIMAL NUMBER: C90984 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 328.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:24 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2296 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 695 ANIMAL NUMBER: C90988 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:50 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0276 .0225 2.0831 1.3893 1.3243 19.8542 1.1918 .6098 1.5892 1.5609 .0075 .0076 .0093 % .0076 % .7021 % .4683 % .4463 % 6.6917 % .4017 % .2055 % .5356 % .5261 % .0025 % .0026 % OBSERVATIONS NECROPSY .0132 .0108 1.0000 .6669 .6357 9.5311 .5721 .2927 .7629 .7493 .0036 .0036 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -LARGE; ALL LOBES -DIFFUSELY DARK; ALL LOBES: DARK BROWN CAVITY, ABDOM (PC) : -EDEMA; ADIPOSE TISSUE SURROUNDING LEFT KIDNEY: CLEAR GELATINOUS AND EDEMATOUS (CAP 18) LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2297 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 696 ANIMAL NUMBER: C90988 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 296.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:50 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2298 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 697 ANIMAL NUMBER: C90993 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/19/98 STUDY DAY OF DEATH: 53 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/19/98 16:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: THICKENED -MOTTLED; ALL LOBES: DARK BROWN AND TAN LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED AND DRY -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -DISCHARGE-NASAL; RED AND DRY -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -GI TRACT-DARK FLUID CONTENT; ENTIRE GI TRACT: DARK BROWN, TENACIOUS SEMIFLUID HISTOPATHOLOGY LIVER (LI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL LUNG (LU) : >UNREMARKABLE CAUSE OF DEATH (CD) : -HEPATIC NECROSIS,-PRESENT 2299 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 698 ANIMAL NUMBER: C90993 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/19/98 STUDY DAY OF DEATH: 53 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/19/98 16:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2300 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 699 ANIMAL NUMBER: C90996 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 397.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:08 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0322 .0309 2.1902 1.7846 1.9303 27.3204 1.6624 .7825 1.9606 1.9131 .0096 .0108 .0081 % .0078 % .5517 % .4495 % .4862 % 6.8817 % .4187 % .1971 % .4939 % .4819 % .0024 % .0027 % OBSERVATIONS NECROPSY .0147 .0141 1.0000 .8148 .8813 12.4739 .7590 .3573 .8952 .8735 .0044 .0049 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -LARGE; ALL LOBES GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE KIDNEY (KD) : -CYST,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2301 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 700 ANIMAL NUMBER: C90996 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 397.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:08 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2302 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 701 ANIMAL NUMBER: C91003 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/98 STUDY DAY OF DEATH: 45 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/98 11:40 PROSECTOR: NANCY DIEDRICH RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: THICKENED -MOTTLED; ALL LOBES: RED, BROWN AND TAN LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN FOCI, UP TO 1 MM IN DIAMETER GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED AND MOIST MATERIAL -DISCHARGE-NASAL; DARK RED AND MOIST MATERIAL -MALOCCLUSION; BILATERAL: UPPER INCISORS; UPPER LEFT INCISOR LOOSE AND EASILY MANIPULATED HISTOPATHOLOGY LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -HEPATIC NECROSIS,-PRESENT 2303 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 702 ANIMAL NUMBER: C91003 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/98 STUDY DAY OF DEATH: 45 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/98 11:40 PROSECTOR: NANCY DIEDRICH RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2304 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 703 ANIMAL NUMBER: C91005 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 295.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:25 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 15.6214 ------------------------------------- ------------------------------- 5.2793 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2305 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 704 ANIMAL NUMBER: C91005 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 295.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:25 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2306 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 705 ANIMAL NUMBER: C91017 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 329.5 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:45 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A T1 U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) LAST CLINICAL OBSERVATIONS .0318 .0347 2.1473 1.4601 1.6177 22.0840 1.3642 .6191 1.7923 1.8783 .0015 .0155 .0097 % .0105 % .6517 % .4431 % .4910 % 6.7023 % .4140 % .1879 % .5439 % .5700 % .0005 % .0047 % OBSERVATIONS NECROPSY .0148 .0162 1.0000 .6800 .7534 10.2845 .6353 .2883 .8347 .8747 .0007 .0072 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN EXCLUDE WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -LARGE; ALL LOBES STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2307 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 706 ANIMAL NUMBER: C91017 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 329.5 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:45 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE) 2308 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 707 ANIMAL NUMBER: C91024 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 268.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:37 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 13.4285 ------------------------------------- ------------------------------- 4.9957 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2309 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 708 ANIMAL NUMBER: C91024 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 268.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:37 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2310 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 709 ANIMAL NUMBER: C91029 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/98 STUDY DAY OF DEATH: 49 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/15/98 9:06 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; CONVULSIONS; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; VOCALIZATION; VERIFIED AT NECROPSY: NOT APPLICABLE >SACRIFICE; SACRIFICE APPROVED BY; STUDY DIRECTOR; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; UNKNOWN; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 3 X 1 MM GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MATERIAL -BLOOD FILM TAKEN RED, DRY HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-SLIGHT, FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 2311 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 710 ANIMAL NUMBER: C91029 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/98 STUDY DAY OF DEATH: 49 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/15/98 9:06 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2312 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 711 ANIMAL NUMBER: C91033 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:46 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 16.2783 ------------------------------------- ------------------------------- 5.1416 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2313 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 712 ANIMAL NUMBER: C91033 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:46 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2314 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 713 ANIMAL NUMBER: C91037 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 340.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:18 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) ------------------------------- 17.0914 ------------------------------------- ------------------------------- 5.0195 % ------------------------------------- ------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2315 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 714 ANIMAL NUMBER: C91037 SEX: MALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 340.5 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:18 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2316 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 715 ANIMAL NUMBER: C91041 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 440.0 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRALCERVICAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 9 X 11 MM, FIRM, RED, AND TAN STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE BROWN ERODED AREAS, UP TO 2 X 3 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, DIFFUSE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2317 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 716 ANIMAL NUMBER: C91041 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 440.0 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY RED AND BROWN MATERIAL -ANIMAL THIN LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION MAMMARY, MALE (MM) : -PIGMENT,-SLIGHT, MULTI-FOCAL 2318 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 717 ANIMAL NUMBER: C91041 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 440.0 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2319 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 718 ANIMAL NUMBER: C91042 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/06/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PANCREAS (PA) : -MASS(ES); ATTACHED TO CRANIAL END: SINGLE DARK RED AND FIRM MASS, 4 MM IN DIAMETER; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (PANCREATIC) NOT IDENTIFIED LUNG (LU) : -UNCOLLAPSED; ALL LOBES PITUITARY (PI) : -LARGE; 4 X 4 X 3 MM; APPEARS SWOLLEN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MODERATE, MULTI FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2320 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 719 ANIMAL NUMBER: C91042 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/06/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AREAS, UP TO 2 X 1 MM MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGIONS: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 X 2 MM (CAP 22) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DRY, TAN CRUSTED AREA, 2 X 1 MM; COLLECTED GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ALL REGIONS, DIFFUSE: DRY, RED MATERIAL STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, -PIGMENT,-MINIMAL, MULTI-FOCAL FOCAL 2321 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 720 ANIMAL NUMBER: C91042 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/06/99 STUDY DAY OF DEATH: 650 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/06/99 13:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2322 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 721 ANIMAL NUMBER: C91043 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 940.8 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:47 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 75; 80 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MUSCLE, SKELETAL (SM) : -MASS(ES); INVOLVING ENTIRE RIGHT LEG: SINGLE MASS, 80 MM IN DIAMETER, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED INTACT WITH LEG; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2323 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 722 ANIMAL NUMBER: C91043 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 940.8 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:47 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2324 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 723 ANIMAL NUMBER: C91044 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/99 STUDY DAY OF DEATH: 443 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 368.5 GRAMS DATE AND TIME OF NECROPSY: 04/13/99 11:38 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 12 X 5 MM; BOTH ARE SOFT PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED, BROWN, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2325 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 724 ANIMAL NUMBER: C91044 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/99 STUDY DAY OF DEATH: 443 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 368.5 GRAMS DATE AND TIME OF NECROPSY: 04/13/99 11:38 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 2 X 1 MM STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE BROWN, SLIGHTLY RAISED AREA, 1 MM IN DIAMETER SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS UP TO 12 X 4 X 2 MM EPIDIDYMIDES (EP) : -SMALL; BILATERAL: EACH IS 35 X 10 X 3 MM; BOTH ARE SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED, DRY, CRUSTED MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL -ANIMAL THIN STOMACH, NONGL (SU) : >UNREMARKABLE PROSTATE (PR) : -MINERALIZATION, LUMINAL,-MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-SLIGHT, MULTI-FOCAL >NOTE:>MAMMARY GLAND WAS PRESENT ON SKIN SECTION 2326 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 725 ANIMAL NUMBER: C91044 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/99 STUDY DAY OF DEATH: 443 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 368.5 GRAMS DATE AND TIME OF NECROPSY: 04/13/99 11:38 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2327 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 726 ANIMAL NUMBER: C91045 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/13/99 STUDY DAY OF DEATH: 353 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 535.8 GRAMS DATE AND TIME OF NECROPSY: 01/13/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 8 X 8 MM, RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 5 X 1 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2328 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 727 ANIMAL NUMBER: C91045 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/13/99 STUDY DAY OF DEATH: 353 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 535.8 GRAMS DATE AND TIME OF NECROPSY: 01/13/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK RED AND DRY MATERIAL 2329 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 728 ANIMAL NUMBER: C91045 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/13/99 STUDY DAY OF DEATH: 353 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 535.8 GRAMS DATE AND TIME OF NECROPSY: 01/13/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2330 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 729 ANIMAL NUMBER: C91046 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/98 STUDY DAY OF DEATH: 44 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: 358.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/98 8:05 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 7 X 2 MM; COLLECTED ON ROUTINE SECTIONS THYMUS (TH) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MODERATE MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, TYPE II PNEUMOCYTE,SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -HEPATIC NECROSIS,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2331 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 730 ANIMAL NUMBER: C91046 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/10/98 STUDY DAY OF DEATH: 44 STUDY WEEK OF DEATH: 7 TERMINAL BODY WEIGHT: 358.1 GRAMS DATE AND TIME OF NECROPSY: 03/10/98 8:05 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2332 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 731 ANIMAL NUMBER: C91047 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 677.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2333 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 732 ANIMAL NUMBER: C91047 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 677.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:45 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) 2334 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 733 ANIMAL NUMBER: C91048 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 490.5 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; GENERALIZED; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 6 X 8 MM, SOFT, RED, DARK RED, AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -INFARCT,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2335 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 734 ANIMAL NUMBER: C91048 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 490.5 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN FOCI, PINPOINT AND UP TO 1 MM IN DIAMETER STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 1 X 2 MM -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY BROWN MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY BROWN MATERIAL -ANIMAL THIN STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2336 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 735 ANIMAL NUMBER: C91048 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 554 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 490.5 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2337 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 736 ANIMAL NUMBER: C91049 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/98 STUDY DAY OF DEATH: 295 STUDY WEEK OF DEATH: 43 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/98 12:27 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; SWOLLEN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE NEAR LEFT TESTIS: SINGLE MASS, 15 X 7 X 4 MM, RED, TAN, AND SOFT; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (LUMBAR) NORMAL (CAP 21) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -VACUOLATION,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC,-MODERATELY SEVERE, DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -NECROSIS, ANAL MUSCULATURE,-PRESENT CAVITY, ABDOM (PC) : -NECROSIS, FAT,-PRESENT 2338 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 737 ANIMAL NUMBER: C91049 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/98 STUDY DAY OF DEATH: 295 STUDY WEEK OF DEATH: 43 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/98 12:27 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY ANUS (AN) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, 12 MM IN DIAMETER, RED, GRAY, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH RECTUM; REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -DISCHARGE-NASAL; DARK RED AND DRY MATERIAL -GI TRACT-LARGE LUMEN; JEJUNUM TO ANUS: UP TO 40 MM IN DIAMETER -GI TRACT-DARK DRY CONTENT; RECTUM TO ANUS: DARK GREEN AND IMPACTED MATERIAL ANUS (AN) : -NECROSIS, MUSCULAR,-MODERATELY SEVERE, MULTI-FOCAL 2339 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 738 ANIMAL NUMBER: C91049 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/16/98 STUDY DAY OF DEATH: 295 STUDY WEEK OF DEATH: 43 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/16/98 12:27 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 2340 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 739 ANIMAL NUMBER: C91050 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 15:56 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); DORSAL; WEEK 95; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -CALCULUS(I); BILATERAL, WITHIN PELVIS: MULTIPLE TAN AND FIRM CALCULI, UP TO 10 MM IN DIAMETER HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-SLIGHT, FOCAL -VASCULARIZATION, CORNEAL,-SLIGHT, FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SEVERE, MULTI FOCAL -NECROSIS, PAPILLARY,-MODERATELY SEVERE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL -PYELONEPHRITIS,-MODERATELY SEVERE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -INFLAMMATION, VASCULAR,-MODERATE, MULTI-FOCAL LUNG (LU) : -EDEMA, ALVEOLAR,-MODERATE, FOCAL 2341 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 740 ANIMAL NUMBER: C91050 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 15:56 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); MID DORSAL THORACIC REGION: SINGLE TAN AND RAISED AREA, 7 X 6 X 4 MM (CAP 22) URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: SINGLE TAN AND FIRM CALCULUS, 6 MM IN DIAMETER -DIFFUSELY RED; DARK RED LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 15 X 10 X 7 MM (CAP 24) LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -INFLAMMATION, VASCULAR,-MODERATE, FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE STOMACH, GL (ST) : -EDEMA,-MODERATE, FOCAL SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -UROLITHIASIS,-PRESENT LN, RENAL (RL) : >UNREMARKABLE 2342 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 741 ANIMAL NUMBER: C91050 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 15:56 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TAIL (TI) : -RAISED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED URETER (UE) : -LARGE; BILATERAL: EACH IS UP TO 2 MM IN DIAMETER (CAP 25) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -MALOCCLUSION; BILATERAL, UPPER INCISORS -URINARY BLADDER-LARGE LUMEN; 50 X 40 X 40 MM TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT URETER (UE) : -DILATATION,-MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL 2343 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 742 ANIMAL NUMBER: C91050 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 719 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/14/00 15:56 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2344 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 743 ANIMAL NUMBER: C91051 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 10 X 3 MM; BOTH APPEAR SOFT PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 6 MM, RED, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2345 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 744 ANIMAL NUMBER: C91051 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE DARK RED AND TAN ERODED AREA, 10 X 3 MM -THICKENED WALL; DIFFUSE SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 8 X 1 MM FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL HINDFEET: SINGLE DARK RED, ABRADED AREA ON EACH, LEFT IS 5 X 3 MM, RIGHT IS 7 MM IN DIAMETER; BOTH COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST -BLOOD FILM TAKEN -ANIMAL THIN PERIANAL: HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, NONGL (SU) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -ULCERATION,-SEVERE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MINIMAL, MULTI-FOCAL -FIBROSIS,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2346 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 745 ANIMAL NUMBER: C91051 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 482.3 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:14 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SM), 2347 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 746 ANIMAL NUMBER: C91052 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 597.7 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:32 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, RED, DARK RED, AND SOFT FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: SINGLE RED, DRY, CRUSTED AREA, 4 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -PYELONEPHRITIS,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -INFLAMMATION, CHRONIC ACTIVE,-SLIGHT, FOCAL 2348 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 747 ANIMAL NUMBER: C91052 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 597.7 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:32 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2349 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 748 ANIMAL NUMBER: C91053 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/28/99 STUDY DAY OF DEATH: 580 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/28/99 14:45 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >HOUSING,FOOD,WATER & MAINT.; TEETH CUT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; KIDNEY (KD) : -DIFFUSELY LIGHT; ALL LOBES: TAN BILATERAL: TAN LUNG (LU) : -MOTTLED; ALL LOBES: RED, DARK RED, TAN AND DARK BROWN GENERAL COMMENT (GC) : -DISCHARGE-NASAL; MOIST BROWN MATERIAL HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LUNG (LU) : >UNREMARKABLE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL JEJUNUM (JE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 2350 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 749 ANIMAL NUMBER: C91053 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/28/99 STUDY DAY OF DEATH: 580 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/28/99 14:45 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2351 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 750 ANIMAL NUMBER: C91054 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND BROWN ADRENAL, CORTEX (AC) : -RAISED AREA(S); RIGHT, CORTICAL SURFACE: SINGLE DARK RED AREA, 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 7 X 5 X 3 MM, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : >TISSUE MISSING >NOTE:>ADRENAL CORTICAL MASS DESCRIBED GROSSLY WAS A MALIGNANT PHEOCHROMOCYTOMA OF THE ADRENAL MEDULLA ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2352 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 751 ANIMAL NUMBER: C91054 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); VENTRAL ABDOMINAL REGION EXTENDING TO LEFT LATERAL LUMBAR REGIONS: MULTIPLE RED AND TAN CRUSTED AREAS, UP TO 20 X 20 MM (CAP 22) PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM MAMMARY, MALE (MM) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 23) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE RED, BROWN AND RAISED AREA, 10 X 7 X 4 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE SKIN (SK) : -ACUTE INFLAMMATORY EXUDATE,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -ULCERATION,-MINIMAL, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, MULTI FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL -PIGMENT,-SLIGHT, FOCAL 2353 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 752 ANIMAL NUMBER: C91054 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1042.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) 2354 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 753 ANIMAL NUMBER: C91055 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:19 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL LOBE, NEAR MARGIN: MULTIPLE TAN AREAS UP TO 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -ROUGH SURFACE, DIFFUSE; ALL LOBES KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL HISTOPATHOLOGY BRAIN (BR) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -NECROSIS, GRAY MATTER,-MODERATE, MULTI FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT LIVER (LI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, TUBULAR,-SLIGHT, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -NECROSIS, CORTICAL,-SLIGHT, FOCAL -VACUOLATION,-MODERATE, FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -NECROSIS,-SLIGHT, MULTI-FOCAL 2355 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 754 ANIMAL NUMBER: C91055 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:19 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); DORSAL SURFACE OF RIGHT CAUDAL LOBE: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 1 MM URINARY BLADDER (UB) : -DIFFUSELY RED; DARK RED PROSTATE (PR) : -MOTTLED; DARK RED, BROWN AND TAN SEMINAL VESICLES (SV) : -MOTTLED; BROWN AND TAN LUNG (LU) : >UNREMARKABLE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT, FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL -ULCERATION,-MODERATE, FOCAL CECUM (CE) : -HEMORRHAGE,-SLIGHT, FOCAL URINARY BLADDER (UB) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -ULCERATION,-SEVERE, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT 2356 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 755 ANIMAL NUMBER: C91055 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:19 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-ORAL; MOIST YELLOW MATERIAL -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMEN EXTENDING TO PERIANAL REGION: DRY AND BROWN MATERIAL -ANIMAL OBESE -URINARY BLADDER-LARGE LUMEN; 40 X 25 X 10 MM; FILLED WITH A DARK RED FLUID HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT 2357 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 756 ANIMAL NUMBER: C91055 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:19 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), EPIDIDYMIDES (EP) 2358 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 757 ANIMAL NUMBER: C91056 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 728.9 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:07 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); HEAD; WEEK 102; 50 X 40MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED -LARGE; RIGHT: 12 X 6 X 5 MM (LEFT IS 5 X 3 X 2 MM); COLLECTED AUDITORY SEB GL (AS) : -MASS(ES); RIGHT: SINGLE MASS, 45 X 40 X 40 MM, RED, DARK RED, TAN, LOBULATED, AND FIRM; ON SECTION EXUDES A LIGHT GREEN SEMISOLID; COLLECTED; REGIONAL LN (SEE MANDIBULAR) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,MODERATE, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL AUDITORY SEB GL (AS) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2359 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 758 ANIMAL NUMBER: C91056 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 728.9 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:07 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; RIGHT UPPER INCISOR -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA) 2360 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 759 ANIMAL NUMBER: C91057 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/99 7:16 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -EROSION(S)/ULCERATION(S); RIGHT, CORTICAL SURFACE: SINGLE TAN AND RED ERODED AREA, 10 X 8 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -PYELONEPHRITIS,-MINIMAL, FOCAL -B-LIPOMA,-NEOPLASM PRESENT TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -HYPERPLASIA, FOLLICULAR CELL,-MINIMAL, FOCAL 2361 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 760 ANIMAL NUMBER: C91057 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/99 7:16 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 5 X 5 MM DARK RED, TAN, AND SOFT SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 6 X 2 MM PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC SEMINAL VESICLES (SV) : ^UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2362 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 761 ANIMAL NUMBER: C91057 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/99 7:16 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2363 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 762 ANIMAL NUMBER: C91058 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 737.0 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; MOUTH; VERIFIED AT NECROPSY: NOT OBSERVED >WART LIKE LESION(S); MOUTH; WEEK 83; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LARGE; 6 X 4 X 3 MM; APPEARS SWOLLEN SKIN (SK) : -MASS(ES); LEFT UPPER LIP: SINGLE TAN AND FIRM WART-LIKE MASS, 2 MM IN DIAMETER (CAP 21); REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL 2364 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 763 ANIMAL NUMBER: C91058 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 737.0 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2365 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 764 ANIMAL NUMBER: C91059 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 526.6 GRAMS DATE AND TIME OF NECROPSY: 08/24/99 14:07 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; GENERALIZED; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 7 X 6 MM, RED, DARK RED, TAN, SOFT, AND LOBULATED STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE DARK BROWN ERODED AREAS, UP TO 3 X 2 MM -THICKENED WALL; DIFFUSE HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-SEVERE, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL -ULCERATION,-SEVERE, MULTI-FOCAL 2366 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 765 ANIMAL NUMBER: C91059 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 526.6 GRAMS DATE AND TIME OF NECROPSY: 08/24/99 14:07 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: BROWN AND MOIST PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2367 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 766 ANIMAL NUMBER: C91059 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 576 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 526.6 GRAMS DATE AND TIME OF NECROPSY: 08/24/99 14:07 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2368 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 767 ANIMAL NUMBER: C91060 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -INFARCT,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2369 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 768 ANIMAL NUMBER: C91060 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 809.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), TESTIS (TE), THYROID (TY), URINARY BLADDER (UB) 2370 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 769 ANIMAL NUMBER: C91061 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/14/99 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/14/99 8:06 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 87; 40 X 50; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -RED FOCUS(I)/AREA(S); ALONG MARGINS OF ALL LOBES: MULTIPLE DARK RED AND NODULAR AREAS, UP TO 20 X 5 MM HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL LUNG (LU) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE, MULTI-FOCAL SKIN (SK) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-HEMANGIOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -HEMANGIOMA,-PRESENT MAMMARY, MALE (MM) : -PIGMENT,-MINIMAL, MULTI-FOCAL 2371 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 770 ANIMAL NUMBER: C91061 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/14/99 STUDY DAY OF DEATH:: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/14/99 8:06 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 60 X 50 X 35 MM, RED, DARK RED, BROWN AND SOFT; SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED TAIL (TI) : -DIFFUSELY DARK; INVOLVING ENTIRE TIP: BLACK; OVERLYING SKIN IS DRY, FIRM, CRUSTED AND RIGID; COLLECTED GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; LARGE AMOUNT OF DARK RED FLUID TAIL (TI) : -NECROSIS,-SEVERE, DIFFUSE 2372 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 771 ANIMAL NUMBER: C91061 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/14/99 STUDY DAY OF DEATH: 627 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/14/99 8:06 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2373 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 772 ANIMAL NUMBER: C91062 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 332.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:20 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); VISCERAL SURFACE OF LEFT LATERAL LOBE: SINGLE TAN FOCUS, 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION TESTIS (TE) : -SMALL; BILATERAL: EACH IS 16 X 9 X 5 MM; BOTH APPEAR SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FIBROSIS,-MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-SLIGHT, FOCAL -HYPERPLASIA, C-CELL,-MODERATELY SEVERE, FOCAL 2374 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 773 ANIMAL NUMBER: C91062 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 332.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:20 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, RED, DARK RED, TAN AND SOFT PROSTATE (PR) : -SMALL; 9 X 8 X 5 MM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 9 X 2 MM GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN, MOIST MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN PERINEUM: PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL 2375 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 774 ANIMAL NUMBER: C91062 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 332.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:20 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (MS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 2376 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 775 ANIMAL NUMBER: C91063 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 324.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 8:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TESTIS (TE) : -SMALL; LEFT: 17 X 9 X 4 MM (RIGHT IS 20 X 13 X 10 MM); LEFT IS SOFT LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 15 X 13 X 10 MM, DARK RED, RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -VACUOLATION,-MODERATE, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE -GRANULOMA, SPERM,-MODERATE, MULTI FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL >NOTE:>LEFT TESTIS LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -MINERALIZATION, LUMINAL,-SLIGHT, MULTI FOCAL 2377 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 776 ANIMAL NUMBER: C91063 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 324.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 8:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH I S 1 5 X 8 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: BROWN AND DRY -ANIMAL THIN SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2378 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 777 ANIMAL NUMBER: C91063 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 324.1 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 8:35 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2379 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 778 ANIMAL NUMBER: C91064 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/05/99 STUDY DAY OF DEATH: 679 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/05/99 14:14 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 15 X 7 MM; COLLECTED ON ROUTINE SECTIONS PARATHYROID (PT) : -LARGE; RIGHT: 2 MM IN DIAMETER (FOR COMPARISON, LEFT IS 1 MM IN DIAMETER) PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 3 X 2 MM HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2380 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 779 ANIMAL NUMBER: C91064 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/05/99 STUDY DAY OF DEATH: 679 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/05/99 14:14 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SG), 2381 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 780 ANIMAL NUMBER: C91065 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:32 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, DARK RED, TAN, AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2382 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 781 ANIMAL NUMBER: C91065 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 719.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:32 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) 2383 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 782 ANIMAL NUMBER: C91066 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 404.3 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 14 X 10 X 8 MM, RED, DARK RED, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL -PIGMENT,-SLIGHT, FOCAL LIVER (LI) : -HEMORRHAGE,-MINIMAL, FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2384 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 783 ANIMAL NUMBER: C91066 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 404.3 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TAIL (TI) : -CRUSTED AREA(S); ENTIRE LENGTH OF TAIL: MULTIPLE TAN, BROWN, AND SLIGHTLY RAISED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN TAIL (TI) : -EROSION,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL 2385 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 784 ANIMAL NUMBER: C91066 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/15/99 STUDY DAY OF DEATH: 598 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 404.3 GRAMS DATE AND TIME OF NECROPSY: 09/15/99 14:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2386 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 785 ANIMAL NUMBER: C91067 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 8:48 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 12 X 8 MM, RED, DARK RED, TAN, AND SOFT SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 20 X 10 X 3 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE 2387 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 786 ANIMAL NUMBER: C91067 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/22/99 STUDY DAY OF DEATH: 696 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/22/99 8:48 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) -ANIMAL OBESE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2388 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 787 ANIMAL NUMBER: C91068 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/98 STUDY DAY OF DEATH: 78 STUDY WEEK OF DEATH: 12 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/13/98 15:01 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 7 X 1 MM -GELATINOUS; BILATERAL: CLEAR GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN HISTOPATHOLOGY LIVER (LI) : -HEMORRHAGE,-MODERATE, FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 2389 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 788 ANIMAL NUMBER: C91068 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/13/98 STUDY DAY OF DEATH: 78 STUDY WEEK OF DEATH: 12 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/13/98 15:01 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2390 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 789 ANIMAL NUMBER: C91069 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/02/99 9:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED FOCUS, 3 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATELY SEVERE, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2391 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 790 ANIMAL NUMBER: C91069 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/02/99 9:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2392 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 791 ANIMAL NUMBER: C91070 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 584.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 6 X 5 X 5 MM, DARK RED, GREY, SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 1 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FIBROSIS,-MINIMAL, FOCAL -PIGMENT,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE, FOCAL -EROSION,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -FIBROSIS,-MODERATELY SEVERE, FOCAL 2393 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 792 ANIMAL NUMBER: C91070 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 584.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND FOOT: SINGLE DARK RED ABRADED AREA 6 X 4 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN FOOT/FOOTPAD(S) (FP) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU), URINARY BLADDER (UB) 2394 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 793 ANIMAL NUMBER: C91071 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:14 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 X 2 MM PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, GREY, FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, DIFFUSE PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2395 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 794 ANIMAL NUMBER: C91071 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 913.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:14 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) 2396 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 795 ANIMAL NUMBER: C91072 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 475.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); MOUTH; WEEK 104; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -MASS(ES); LEFT, INVOLVING ENTIRE ORGAN: SINGLE MASS, 40 X 40 X 32 MM, RED, DARK RED, TAN, VASCULAR, AND FIRM; SAME ON CUT SECTION, COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 3 MM IN DIAMETER (CAP 22) SKIN (SK) : -MASS(ES); LEFT SIDE OF HEAD, NEAR CORNER OF MOUTH: SINGLE MASS, 8 MM IN DIAMETER, DARK RED, AND FIRM; ON SECTION EXUDES A LIGHT GREEN SEMISOLID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL; COLLECTED HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL -M-LIPOSARCOMA,-NEOPLASM PRESENT PANCREAS (PA) : -HYPERTROPHY, VASCULAR,-MODERATE, MULTI FOCAL -INFLAMMATION, VASCULAR,-MINIMAL, MULTI FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL SKIN (SK) : -INFLAMMATION, CHRONIC,-MODERATELY SEVERE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT 2397 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 796 ANIMAL NUMBER: C91072 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 475.9 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:17 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LUNG (LU) 2398 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 797 ANIMAL NUMBER: C91073 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 963.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:30 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER, COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: TWO DARK RED AREAS, EACH IS 2 MM IN DIAMETER FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HIND FOOT: SINGLE DARK RED, MOIST, AND ABRADED AREA, 15 X 14 MM; COLLECTED HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2399 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 798 ANIMAL NUMBER: C91073 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 963.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:30 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; AND BROWN MATERIAL -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE DRY YELLOW THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), THYROID (TY), URINARY BLADDER (UB) 2400 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 799 ANIMAL NUMBER: C91074 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 711 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 587.4 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY DARK; BILATERAL: DARK GREEN TESTIS (TE) : -SMALL; BILATERAL: BOTH ARE 23 X 11 X 4 MM, BOTH APPEAR SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE, MULTI FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL 2401 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 800 ANIMAL NUMBER: C91074 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 711 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 587.4 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN AND BROWN ERODED AREAS, UP TO 5 X 3 MM PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: BOTH ARE 14 X 5 X 2 MM FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT, NEAR JUNCTION OF DIGITS 2,3, AND 4: SINGLE TAN AND BROWN, SLIGHTLY RAISED AREA, 5 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK YELLOW -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-SLIGHT, FOCAL -EROSION,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EXOSTOSES,-SLIGHT, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL 2402 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 801 ANIMAL NUMBER: C91074 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 711 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 587.4 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 14:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2403 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 802 ANIMAL NUMBER: C91075 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 656.3 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 9:52 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPINAL CORD (SC) : >NOTE:>TRIMMING COMMENT: SPINAL CORD NOT FOUND AT TIME OF TRIMMING PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SPINAL CORD (SC) : >TISSUE MISSING LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2404 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 803 ANIMAL NUMBER: C91075 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 656.3 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 9:52 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 15 X 6 MM FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: SINGLE TAN, DRY CRUSTED AREA, 6 X 5 MM; COLLECTED -ABRASION(S)/ULCERATION(S); RIGHT HIND FOOT: SINGLE DARK RED MOIST ABRADED AREA, 5 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERIANAL: DARK YELLOW AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, MOIST MATERIAL -ANIMAL OBESE HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-SLIGHT, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2405 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 804 ANIMAL NUMBER: C91075 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 540 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 656.3 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 9:52 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2406 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 805 ANIMAL NUMBER: C91076 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE LIGHT GREEN AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT THYROID (TY) : -LARGE; LEFT: 8 X 4 X 4 MM (RIGHT IS 5 X 4 X 2 MM) PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 2 X 1 MM HISTOPATHOLOGY LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2407 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 806 ANIMAL NUMBER: C91076 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 10 X 4 X 2 MM; COLLECTED FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: SINGLE DRY RED, TAN CRUSTED AREA, 15 X 10 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE ABDOMINAL REGION: DRY YELLOW MATERIAL -BONE MARROW SMEAR TAKEN LN, MANDIBULAR (MN) : -DILATATION, SINUSOIDAL,-MINIMAL, FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, DIFFUSE FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -EXOSTOSES,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2408 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 807 ANIMAL NUMBER: C91076 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 747.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:50 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), URINARY BLADDER (UB) 2409 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 808 ANIMAL NUMBER: C91077 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 573.5 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, RED, DARK RED, TAN AND FIRM SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL LUNG (LU) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -EDEMA,-MODERATE, MULTI-FOCAL 2410 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 809 ANIMAL NUMBER: C91077 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 573.5 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 35 X 20 X 4 MM LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 9 X 5 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -TEETH MISSING/BROKEN/WORN; MISSING, BILATERAL UPPER INCISORS SEMINAL VESICLES (SV) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL -SECRETION, INCREASED,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, DIFFUSE 2411 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 810 ANIMAL NUMBER: C91077 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 573.5 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:02 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2412 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 811 ANIMAL NUMBER: C91078 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/05/99 7:43 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -LARGE; BILATERAL: EACH IS 35 X 25 X 19 MM TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 7 X 5 MM LUNG (LU) : -UNCOLLAPSED; ALL LOBES -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED FOCI, UP TO 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -HYPERPLASIA, BILE DUCT,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -STASIS, BILE,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL TESTIS (TE) : >UNREMARKABLE LUNG (LU) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2413 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 812 ANIMAL NUMBER: C91078 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/05/99 7:43 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY THYROID (TY) : -LARGE; BILATERAL: EACH IS 9 X 5 X 4 MM PITUITARY (PI) : -MASS(ES); 11 X 9 X 8 MM, RED, DARK RED, GREY, TAN, LOBULATED AND SOFT SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 3 X 3 MM URETER (UE) : -LARGE; BILATERAL: EACH IS UP TO 1 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -GI TRACT-LARGE LUMEN; INVOLVING ENTIRE GI TRACT: UP TO 35 MM IN DIAMETER HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -M-CARCINOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : ^UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT URETER (UE) : >TISSUE MISSING 2414 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 813 ANIMAL NUMBER: C91078 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/05/99 7:43 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2415 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 814 ANIMAL NUMBER: C91079 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 449.6 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:09 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 7 X 7 MM, RED, DARK RED, TAN, AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -LARGE; LEFT: 32 X 18 X 3 MM (RIGHT IS 26 X 12 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT 2416 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 815 ANIMAL NUMBER: C91079 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 449.6 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 13:09 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2417 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 816 ANIMAL NUMBER: C91080 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 11:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); EAR-LEFT; WEEK 65; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); VENTRAL ABDOMINAL; WEEK 76; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED AND SOFT SKIN (SK) : -MASS(ES); MID VENTRAL THORACIC REGION: SINGLE MASS, 15 X 10 X 6 MM, TAN AND HARD; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 24) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT SEMINAL VESICLES (SV) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL 2418 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 817 ANIMAL NUMBER: C91080 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 11:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DRY BROWN CRUSTED AREA, 15 MM IN DIAMETER; SURROUNDING TISSUE APPEARS SWOLLEN; COLLECTED PINNA (PN) : -CRUSTED AREA(S); LEFT, NEAR CANAL: SINGLE DRY BROWN AREA, 3 X 2 X 1 MM (CAP 22) CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -FIBROSIS,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL PINNA (PN) : >UNREMARKABLE 2419 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 818 ANIMAL NUMBER: C91080 SEX: MALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/07/99 11:18 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2420 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 819 ANIMAL NUMBER: C91099 SEX: MALE DOSE GROUP: 7 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/98 STUDY DAY OF DEATH: 39 STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/98 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; ORAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >HOUSING,FOOD,WATER & MAINT.; WATER BOTTLE PLACED ON CAGE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; DEHYDRATED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, PINPOINT AND UP TO 3 X 1 MM GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED, DRY MATERIAL -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL THYROID (TY) : -ECTOPIC THYMUS,-PRESENT STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL JEJUNUM (JE) : >AUTOLYTIC CAUSE OF DEATH (CD) : -HEPATIC NECROSIS,-PRESENT 2421 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 820 ANIMAL NUMBER: C91099 SEX: MALE DOSE GROUP: 7 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/05/98 STUDY DAY OF DEATH: 39 STUDY WEEK OF DEATH: 6 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/98 7:57 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), MAMMARY, MALE (MM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), PENIS (PE), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (SE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 2422 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 821 ANIMAL NUMBER: C91121 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 397.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:02 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST : DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0466 .0388 2.0851 1.3222 1.5011 11.8627 1.9458 .0548 .0515 .5875 .0211 .0171 1.7745 .0117 % .0098 % .5242 % .3324 % .3774 % 2.9821 % .4891 % .0138 % .0129 % .1477 % .0053 % .0043 % .4461 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0223 .0186 1.0000 .6341 .7199 5.6893 .9332 .0263 .0247 .2818 .0101 .0082 .8510 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 1 MM IN DIAMETER LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2423 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 822 ANIMAL NUMBER: C91121 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 397.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:02 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2424 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 823 ANIMAL NUMBER: C91121 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 397.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:02 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2425 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 824 ANIMAL NUMBER: C91122 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 451.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0420 .0248 2.2764 1.3642 1.3425 10.7372 1.5978 .0558 .0565 .6826 .0168 .0214 .4623 .0093 % .0055 % .5046 % .3024 % .2976 % 2.3802 % .3542 % .0124 % .0125 % .1513 % .0037 % .0047 % .1025 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0185 .0109 1.0000 .5993 .5897 4.7167 .7019 .0245 .0248 .2999 .0074 .0094 .2031 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL 2426 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 825 ANIMAL NUMBER: C91122 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 451.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2427 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 826 ANIMAL NUMBER: C91122 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 451.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2428 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 827 ANIMAL NUMBER: C91123 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/09/99 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/09/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 85; 50 X 60MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 94; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 97; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES; MARGINS APPEAR ROUNDED ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 8 X 8 X 6 MM, RIGHT IS 10 X 8 X 8 MM -DIFFUSELY DARK; BILATERAL: DARK BROWN THYMUS (TH) : -MOTTLED; RED, DARK RED, GRAY AND TAN -LARGE; 3 5 X 2 5 X 6 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SEVERE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,SEVERE, MULTI-FOCAL -HEMORRHAGE,-SEVERE, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2429 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 828 ANIMAL NUMBER: C91123 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/09/99 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/09/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 6 X 4 MM, DARK RED, TAN, SOFT AND LOBULATED MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 65 X 55 X 40 MM, TAN, RED, SOFT AND VASCULAR; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -CYST(S); VENTRAL ABDOMINAL REGION: MULTIPLE TAN, CREAMY, SEMIFLUID FILLED CYSTS, UP TO 12 X 10 X 5 MM; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 15 X 13 X 6 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND SOFT; COLLECTED ON ROUTINE SECTION HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC COLON (CO) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 2430 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 829 ANIMAL NUMBER: C91123 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/09/99 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/09/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 50 X 40 X 25 MM, TAN AND SOFT; CUT SURFACE EXUDES A TAN CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (RIGHT INGUINAL) NORMAL (CAP 22) LN, AXILLARY (AX) : -MOTTLED; BILATERAL: TAN AND BROWN (CAP 21) CAUSE OF DEATH (CD) : -MALIGNANT LYMPHOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT LN, AXILLARY (AX) : ^UNREMARKABLE LN, INGUINAL (IN) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 2431 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 830 ANIMAL NUMBER: C91123 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/09/99 STUDY DAY OF DEATH: 683 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/09/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA) 2432 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 831 ANIMAL NUMBER: C91124 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 363.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0661 .0603 2.1807 1.5783 1.6324 11.4796 1.6056 .0650 .0626 .6360 .0129 .0097 1.2016 .0182 % .0166 % .6004 % .4346 % .4494 % 3.1607 % .4421 % .0179 % .0172 % .1751 % .0036 % .0027 % .3308 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0303 .0277 1.0000 .7238 .7486 5.2642 .7363 .0298 .0287 .2916 .0059 .0044 .5510 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ASPECT: TWO BROWN FOCI, BOTH ARE 1 MM IN DIAMETER KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-SLIGHT, FOCAL 2433 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 832 ANIMAL NUMBER: C91124 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 363.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2434 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 833 ANIMAL NUMBER: C91124 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 363.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:53 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2435 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 834 ANIMAL NUMBER: C91125 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 353.9 GRAMS DATE AND TIME OF NECROPSY: 11/27/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 87; 70 X 55MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -HYPERTROPHY, CORTICAL CELL,-MODERATE, FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2436 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 835 ANIMAL NUMBER: C91125 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 353.9 GRAMS DATE AND TIME OF NECROPSY: 11/27/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); RIGHT POSTERIOR ASPECT: SINGLE MASS, 5 X 4 X 2 MM, DARK RED AND SOFT STOMACH, NONGL (SU) : -RAISED AREA(S); MUCOSA: SINGLE WHITE AREA, 7 X 5 X 3 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERINEAL REGION: SINGLE MASS, 65 X 65 X 25 MM, TAN, DARK RED, RED, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, NONGL (SU) : -FIBROSIS,-MODERATELY SEVERE, FOCAL -HYPERKERATOSIS, NONGLANDULAR MUCOSA,MODERATE, DIFFUSE -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT 2437 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 836 ANIMAL NUMBER: C91125 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/27/99 STUDY DAY OF DEATH: 671 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 353.9 GRAMS DATE AND TIME OF NECROPSY: 11/27/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) (SM), 2438 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 837 ANIMAL NUMBER: C91126 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 229.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 7:41 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0378 .0353 1.9637 .9111 .9189 6.8127 1.1246 .0507 .0498 .4642 .0080 .0070 .3980 .0165 % .0154 % .8568 % .3975 % .4009 % 2.9724 % .4907 % .0221 % .0217 % .2025 % .0035 % .0031 % .1736 % .0192 .0180 1.0000 .4640 .4679 3.4693 .5727 .0258 .0254 .2364 .0041 .0036 .2027 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2439 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 838 ANIMAL NUMBER: C91126 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 229.2 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 7:41 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL INFORMATION (XX) : >NOTE:>ANIMAL DIED PRIOR TO EXSANGUINATION ANIMAL NECROPSY PERFORMED OUT OF ORDER DUE TO MORIBUND CONDITION THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2440 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 839 ANIMAL NUMBER: C91127 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 353.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; LATERALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 91; 60 X 50MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 X MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-MINIMAL, FOCAL -ULCERATION, CORNEAL,-MINIMAL, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT, MULTI FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT 2441 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 840 ANIMAL NUMBER: C91127 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 353.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -MASS(ES); LEFT: SINGLE MASS, 15 X 11 X 11 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE RENAL) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 8 X 6 MM, RED, DARK RED, GRAY, LOBULATED AND SOFT SKIN (SK) : -CRUSTED AREA(S); LEFT INGUINAL REGION: SINGLE DRY TAN AND BROWN CRUSTED AREA, 25 X 10 MM (CAP 20) MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 70 X 50 X 30 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-SEVERE, FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE 2442 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 841 ANIMAL NUMBER: C91127 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 353.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CLEAR MUCOID MATERIAL LN, RENAL (RL) : -LARGE; LEFT: 9 X 5 X 5 MM (CAP 21) -DIFFUSELY RED; RIGHT: DARK RED -SMALL; RIGHT: 2 X 1 X 1 MM (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSIED OUT OF ORDER DUE TO MORIBUND CONDITION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2443 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 842 ANIMAL NUMBER: C91127 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 353.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:03 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2444 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 843 ANIMAL NUMBER: C91128 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 554.6 GRAMS DATE AND TIME OF NECROPSY: 07/02/99 9:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2445 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 844 ANIMAL NUMBER: C91128 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 554.6 GRAMS DATE AND TIME OF NECROPSY: 07/02/99 9:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD AND VENTRAL ABDOMINAL REGION: DARK RED AND DRY MATERIAL 2446 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 845 ANIMAL NUMBER: C91128 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 554.6 GRAMS DATE AND TIME OF NECROPSY: 07/02/99 9:58 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2447 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 846 ANIMAL NUMBER: C91129 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:08 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0283 .0247 2.0540 1.3979 1.4025 10.5662 1.2784 .0560 .0486 .5479 .0130 .0045 .6525 .0077 % .0067 % .5591 % .3805 % .3817 % 2.8759 % .3480 % .0152 % .0132 % .1491 % .0035 % .0012 % .1776 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0138 .0120 1.0000 .6806 .6828 5.1442 .6224 .0273 .0237 .2667 .0063 .0022 .3177 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2448 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 847 ANIMAL NUMBER: C91129 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 367.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:08 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2449 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 848 ANIMAL NUMBER: C91130 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/13/99 14:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED, TAN, AND SOFT CERVIX (CV) : -LARGE; 8 X 6 X 5 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL NERVE, SCIATIC (SN) : >TISSUE MISSING PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC CERVIX (CV) : >UNREMARKABLE 2450 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 849 ANIMAL NUMBER: C91130 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/13/99 14:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -DISCHARGE-NASAL; DARK RED AND DRY CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2451 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 850 ANIMAL NUMBER: C91130 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/13/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/13/99 14:44 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2452 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 851 ANIMAL NUMBER: C91131 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 MM IN DIAMETER (LEFT IS 4 X 4 X 3 MM) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 7 MM IN DIAMETER PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN HISTOPATHOLOGY LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -PYELONEPHRITIS,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : >UNREMARKABLE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE 2453 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 852 ANIMAL NUMBER: C91131 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -LARGE, DIFFUSE; BILATERAL: LEFT HORN IS 70 X UP TO 15 X 3 MM, RIGHT HORN IS 7 0 X 1 0 X 3 MM LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DARK RED AND DRY AREA, 5 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT, MULTI-FOCAL -HYPERPLASIA, ENDOMETRIAL,-MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MINIMAL, MULTI FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2454 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 853 ANIMAL NUMBER: C91131 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 341.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:43 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2455 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 854 ANIMAL NUMBER: C91132 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:34 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 64; 80 X 90MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 5 X 3 MM, RED, DARK RED, TAN, AND SOFT SKIN (SK) : >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO LARGE MASS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL -ULCERATION,-SEVERE, FOCAL MAMMARY, FEMALE (MF) : -MINERALIZATION,-MODERATE, MULTI-FOCAL 2456 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 855 ANIMAL NUMBER: C91132 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:34 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 105 X 64 X 36 MM, TAN, RED, DARK RED, BROWN, LOBULATED, AND CYSTIC; ON SECTION EXUDES A DARK BROWN FLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 28 X 15 X 13 MM, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) >NOTE:>ROUTINE SECTION COLLECTED FROM RIGHT INGUINAL REGION DUE TO LARGE MASS UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER MAMMARY, FEMALE (MF) : -PIGMENT,-MODERATELY SEVERE, MULTI FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION LN, AXILLARY (AX) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2457 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 856 ANIMAL NUMBER: C91132 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:34 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 13 X 7 X 2 MM (RIGHT IS UP TO 3 X 2 X 2 MM) -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LN, INGUINAL (IN) : >TISSUE MISSING 2458 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 857 ANIMAL NUMBER: C91132 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 438.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:34 PROSECTOR: TONY HANFELD RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2459 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 858 ANIMAL NUMBER: C91133 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 420.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0276 .0300 2.2072 1.1849 1.2369 10.3579 1.7630 .0847 .0766 .5825 .0090 .0192 .8435 .0066 % .0071 % .5253 % .2820 % .2944 % 2.4650 % .4196 % .0202 % .0182 % .1386 % .0021 % .0046 % .2007 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0125 .0136 1.0000 .5368 .5604 4.6928 .7987 .0384 .0347 .2639 .0041 .0087 .3822 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED ADRENAL, CORTEX (AC) : >NOTE:>TRIM COMMENT: MISSING ONE ADRENAL AT TIME OF TRIMMING KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ONE EXAMINED,-PRESENT >NOTE:>ONE EXAMINED ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT >NOTE:>ONE EXAMINED CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2460 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 859 ANIMAL NUMBER: C91133 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 420.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN 2461 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 860 ANIMAL NUMBER: C91133 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 420.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2462 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 861 ANIMAL NUMBER: C91134 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:24 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 51; 60 X 40MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; LIMBSFRONT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 10 X 8 MM, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 60 X 50 X 25 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; SOME AREAS EXUDE A TAN AND CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2463 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 862 ANIMAL NUMBER: C91134 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:24 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); RIGHT PERIANAL REGION: SINGLE CYST, 13 MM IN DIAMETER, FILLED WITH A TAN CREAMY SEMIFLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2464 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 863 ANIMAL NUMBER: C91134 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:24 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2465 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 864 ANIMAL NUMBER: C91135 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 192.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:13 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 6.4555 ------------------------------------------- ------------------------------- 3.3552 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2466 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 865 ANIMAL NUMBER: C91135 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 192.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:13 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2467 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 866 ANIMAL NUMBER: C91136 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 417.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 12:14 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 7 MM, DARK RED, GREY, AND SOFT UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 25 X 8 X 8 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -DILATATION,-SEVERE, DIFFUSE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2468 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 867 ANIMAL NUMBER: C91136 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 417.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 12:14 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED AND DRY AREA, 7 X 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED MOIST MATERIAL GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2469 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 868 ANIMAL NUMBER: C91136 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 417.0 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 12:14 PROSECTOR: TODD ACKER RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2470 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 869 ANIMAL NUMBER: C91137 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 354.1 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:23 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; PAW-HINDRIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, DARK RED AND SOFT STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN INDISTINCT AREAS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, CORTICAL CELL,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 2471 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 870 ANIMAL NUMBER: C91137 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 354.1 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:23 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY JOINT, OTHER (JT) : -LARGE; RIGHT HINDFOOT: UP TO 12 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; HEAD: DRY RED MATERIAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, FOCAL CERVIX (CV) : -DILATATION,-SLIGHT, FOCAL -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT JOINT, OTHER (JT) : -EXOSTOSES,-SEVERE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL 2472 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 871 ANIMAL NUMBER: C91137 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 354.1 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:23 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2473 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 872 ANIMAL NUMBER: C91138 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 418.8 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 99; 50 X 70MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 5 X 4 MM, DARK RED, TAN, AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 2474 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 873 ANIMAL NUMBER: C91138 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 418.8 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 90 X 60 X 30 MM, TAN, VASCULAR, AND SOFT; ON SECTION EXUDES A TAN CREAMY SEMIFLUID; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CERVIX (CV) : -PIGMENT,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2475 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 874 ANIMAL NUMBER: C91138 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 418.8 GRAMS DATE AND TIME OF NECROPSY: 10/27/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2476 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 875 ANIMAL NUMBER: C91139 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 432 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/02/99 10:02 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 11 MM, FIRM, RED, DARK RED AND TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DRY RED MATERIAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2477 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 876 ANIMAL NUMBER: C91139 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/02/99 STUDY DAY OF DEATH: 432 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/02/99 10:02 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2478 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 877 ANIMAL NUMBER: C91140 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 301.6 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:05 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0531 .0479 2.0571 1.0500 1.0632 8.5377 1.4318 .0763 .0930 .4953 .0103 .0099 .5868 .0176 % .0159 % .6821 % .3481 % .3525 % 2.8308 % .4747 % .0253 % .0308 % .1642 % .0034 % .0033 % .1946 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0258 .0233 1.0000 .5104 .5168 4.1504 .6960 .0371 .0452 .2408 .0050 .0048 .2853 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2479 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 878 ANIMAL NUMBER: C91140 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 301.6 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:05 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2480 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 879 ANIMAL NUMBER: C91141 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/06/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/06/99 7:24 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); LIMB-HIND-RIGHT; WEEK 71; 20 X 30MM; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, DARK RED AND SOFT STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AND DARK BROWN AREAS, UP TO 4 X 3 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE MULTI-FOCAL KIDNEY (KD) : -CYST,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL MULTI-FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL 2481 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 880 ANIMAL NUMBER: C91141 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/06/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/06/99 7:24 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 40 X 40 X 20 MM, RED, DARK RED, GRAY, TAN, LOBULATED, AND FIRM; ON SECTION, EXUDES A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CERVIX (CV) : -LARGE; 10 X 9 X 8 MM GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT CERVIX (CV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2482 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 881 ANIMAL NUMBER: C91141 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/06/99 STUDY DAY OF DEATH: 558 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/06/99 7:24 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2483 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 882 ANIMAL NUMBER: C91142 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:48 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); INGUINAL REGION-RIGHT; WEEK 72; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL ADRENAL, CORTEX (AC) : -LARGE; LEFT: 11 MM IN DIAMETER (FOR COMPARISON, RIGHT IS 5 X 3 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -CYST,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -CYST,-MINIMAL, FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL 2484 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 883 ANIMAL NUMBER: C91142 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:48 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 7 X 5 MM, RED, DARK RED, TAN, LOBULATED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 25 X 20 X 10 MM, RED, TAN, VASCULAR AND FIRM; ON SECTION EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK RED AND BROWN, DRY, MATERIAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2485 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 884 ANIMAL NUMBER: C91142 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:48 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) 2486 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 885 ANIMAL NUMBER: C91143 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 174.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 7:25 PROSECTOR: DAVID SCHUETTE RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 6.8425 ------------------------------------------- ------------------------------- 3.9325 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2487 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 886 ANIMAL NUMBER: C91143 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 174.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 7:25 PROSECTOR: DAVID SCHUETTE RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2488 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 887 ANIMAL NUMBER: C91144 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >DISCHARGE; GENITAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 3 MM IN DIAMETER, RIGHT IS 10 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 25 X 15 X 12 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGE,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2489 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 888 ANIMAL NUMBER: C91144 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CERVIX (CV) : -LARGE; TRIMMING COMMENT: UP TO 8 MM IN DIAMETER; TRIMMED IN ROUTINE SECTION UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: REDDISH-BROWN FLUID -LARGE, DIFFUSE; BILATERAL: EACH HORN IS UP TO 110 X 25 X 25 MM GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; RED -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MOIST AND CERVIX (CV) : -CYST,-PRESENT UTERUS (UT) : -DILATATION,-SEVERE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2490 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 889 ANIMAL NUMBER: C91144 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) 2491 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 890 ANIMAL NUMBER: C91145 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:07 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0402 .0359 2.0585 .9720 1.0001 7.5230 1.4443 .0866 .0878 .5149 .0106 .0085 .7386 .0152 % .0136 % .7771 % .3669 % .3775 % 2.8399 % .5452 % .0327 % .0331 % .1944 % .0040 % .0032 % .2788 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0195 .0174 1.0000 .4722 .4858 3.6546 .7016 .0421 .0427 .2501 .0051 .0041 .3588 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2492 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 891 ANIMAL NUMBER: C91145 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 264.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:07 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2493 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 892 ANIMAL NUMBER: C91146 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 361.1 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:04 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 71; 10 X 15MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 6 MM, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : >TISSUE MISSING 2494 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 893 ANIMAL NUMBER: C91146 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 361.1 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:04 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, AXILLARY (AX) : -CYST(S); RIGHT, INVOLVING ENTIRE NODE: SINGLE CLEAR FLUID FILLED CYST, 10 MM IN DIAMETER; POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN OFF AT BASE LN, AXILLARY (AX) : >NOTE:>AXILLARY LYMPH NODE CYSTS DESCRIBED GROSSLY WAS MAMMARY GALACTOCELES 2495 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 894 ANIMAL NUMBER: C91146 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 361.1 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:04 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2496 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 895 ANIMAL NUMBER: C91147 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 323.9 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:54 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -CYST(S); RIGHT, CORTICAL SURFACE: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER LN, MESENTERIC (MS) : -MOTTLED; RED AND TAN PITUITARY (PI) : -MASS(ES); 17 X 15 X 10 MM, DARK RED TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -CYST,-MODERATE, FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : >UNREMARKABLE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL 2497 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 896 ANIMAL NUMBER: C91147 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 323.9 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:54 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); PERINEAL REGION: SINGLE DARK RED FLUID FILLED CYST, 15 MM IN DIAMETER; COLLECTED UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL, DARK RED AND DRY -ANIMAL THIN MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2498 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 897 ANIMAL NUMBER: C91147 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 323.9 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:54 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2499 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 898 ANIMAL NUMBER: C91148 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 598.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:07 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 95; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 98; 30 X 25MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 98; 40 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 102; 50 X 45MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -DIFFUSELY DARK; RIGHT: DARK RED OVARY (OV) : -CYST(S); LEFT: SINGLE GREEN FLUID FILLED CYST, 14 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -THROMBUS,-SLIGHT, FOCAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL 2500 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 899 ANIMAL NUMBER: C91148 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 598.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:07 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK RED LINEAR AREA, 6 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 45 X 37 X 35 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; POSSIBLY CONSISTENT WITH MASS (AXILLARY REGION, RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 50 X 40 X 35 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL, RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #3; RIGHT INGUINAL REGION: SINGLE PEDUNCULATED MASS, 120 X 65 X 24 MM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE; POSSIBLY CONSISTENT WITH MASSES (VENTRAL ABDOMINAL AND INGUINAL) AS NOTED IN CLINICAL OBSERVATIONS; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 2501 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 900 ANIMAL NUMBER: C91148 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 598.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:07 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; RIGHT: UP TO 10 X 8 X 3 MM (LEFT IS 8 X 4 X 2 MM) (CAP 20) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DARK RED CRUSTED AREA, 10 X 8 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, FOCAL -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -EXOSTOSES,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2502 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 901 ANIMAL NUMBER: C91148 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 598.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:07 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) 2503 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 902 ANIMAL NUMBER: C91149 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 360.4 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 13:10 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 51; 50 X 55MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 82; 35 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; LIMB-FRONTLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : >NOTE:>TRIMMING COMMENT: MISSING FROM WET TISSUE PITUITARY (PI) : -MASS(ES); 9 X 7 X 7 MM, RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 20 X 16 X 8 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A DARK RED AND TAN ERODED AREA, 9 X 5 MM (CAP 22); REGIONAL LN (AXILLARY) (CAP 21); OVERLYING SKIN, POSSIBLY CONSISTENT WITH SORE/SCAB (LIMB-FRONT-LEFT) NOTED IN CLINICAL OBSERVATIONS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : >TISSUE MISSING PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 2504 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 903 ANIMAL NUMBER: C91149 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 360.4 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 13:10 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 55 X 50 X 35 MM (40.8 GRAMS), RED, TAN, GRAY, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2505 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 904 ANIMAL NUMBER: C91149 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 360.4 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 13:10 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2506 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 905 ANIMAL NUMBER: C91150 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 381.6 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 10:33 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 62; 45 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRALCERVICAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL MID THORACIC REGION: SINGLE MASS, 50 X 40 X 10 MM, RED, DARK RED, TAN, BROWN AND SOFT; SAME ON CUT SURFACE; SOME AREAS EXUDE A BROWN CASEOUS MATERIAL; OVERLYING SKIN HAS A SINGLE ABRADED AREA, 10 X 6 MM; COLLECTED; REGIONAL LN (SEE MANDIBULAR) LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: COLLECTED LN, AXILLARY (AX) : -LARGE; LEFT: 10 X 4 X 3 MM (FOR COMPARISON, RIGHT IS 8 X 3 X 2 MM) (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL 2507 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 906 ANIMAL NUMBER: C91150 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/05/99 STUDY DAY OF DEATH: 526 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 381.6 GRAMS DATE AND TIME OF NECROPSY: 07/05/99 10:33 PROSECTOR: TROY ELLENBECKER RECORDER: TROY ELLENBECKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB),CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2508 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 907 ANIMAL NUMBER: C91151 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 297.9 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:07 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, TAN, RED AND FIRM STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN, SLIGHTLY RAISED AREAS, UP TO 2 X 1 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2509 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 908 ANIMAL NUMBER: C91151 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 297.9 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:07 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN, SLIGHTLY RAISED AREAS, UP TO 2 X 1 MM VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MUCOID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, DARK RED MATERIAL -ANIMAL THIN STOMACH, NONGL (SU) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2510 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 909 ANIMAL NUMBER: C91151 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 297.9 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:07 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2511 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 910 ANIMAL NUMBER: C91152 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 402.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST : DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0403 .0506 2.3005 1.3529 1.3051 11.2455 1.6125 .0625 .0565 .6141 .0144 .0163 1.2985 .0100 % .0126 % .5717 % .3362 % .3243 % 2.7946 % .4007 % .0155 % .0140 % .1526 % .0036 % .0041 % .3227 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0175 .0220 1.0000 .5881 .5673 4.8883 .7009 .0272 .0246 .2669 .0063 .0071 .5644 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND TAN LIVER (LI) : ^UNREMARKABLE KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 2512 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 911 ANIMAL NUMBER: C91152 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 402.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2513 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 912 ANIMAL NUMBER: C91152 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 402.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 10:33 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER(JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON(TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2514 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 913 ANIMAL NUMBER: C91153 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 700.3 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:31 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 60; 40 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 5 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN PELVIS: MULTIPLE PINPOINT TAN GRANULES PITUITARY (PI) : -MASS(ES); SINGLE MASS, 4 X 6 X 8 MM, SOFT, RED, DARK RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -TELANGIECTASIS,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL 2515 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 914 ANIMAL NUMBER: C91153 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 700.3 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:31 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 20 X 32 X 50 MM, FIRM, LOBULATED, RED, DARK RED, TAN; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A MOIST, RED AND BROWN ERODED AREA, 7 X 12 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED WITH ROUTINE SECTION LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 21) LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN SKIN (SK) : -ULCERATION,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -NECROSIS,-MODERATELY SEVERE, MULTI FOCAL -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, AXILLARY (AX) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE, MULTI-FOCAL 2516 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 915 ANIMAL NUMBER: C91153 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 700.3 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 15:31 PROSECTOR: DENNIS HOFFMAN RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2517 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 916 ANIMAL NUMBER: C91154 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/20/99 14:33 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL HISTOPATHOLOGY BRAIN (BR) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -M-ASTROCYTOMA,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -ASTROCYTOMA,-PRESENT 2518 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 917 ANIMAL NUMBER: C91154 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/20/99 14:33 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2519 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 918 ANIMAL NUMBER: C91155 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:10 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0403 .0436 1.8827 1.0812 1.1129 8.6177 1.1623 .0753 .0530 .4785 .0163 .0099 .7963 .0146 % .0158 % .6812 % .3912 % .4026 % 3.1178 % .4205 % .0272 % .0192 % .1731 % .0059 % .0036 % .2881 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0214 .0232 1.0000 .5743 .5911 4.5773 .6174 .0400 .0282 .2542 .0087 .0053 .4230 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2520 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 919 ANIMAL NUMBER: C91155 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:10 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2521 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 920 ANIMAL NUMBER: C91156 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 575.0 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:42 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 8 MM, DARK RED, GRAY AND SOFT UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: DARK RED FLUID HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT 2522 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 921 ANIMAL NUMBER: C91156 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 575.0 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:42 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -LARGE, DIFFUSE; BILATERAL: EACH HORN IS DISTENDED UP TO 15 MM IN DIAMETER WITH A DARK RED FLUID -MASS(ES); ATTACHED TO SEROSAL SURFACE OF PROXIMAL LEFT HORN: SINGLE MASS, 15 MM IN DIAMETER, DARK RED, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; RED -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -HEMANGIOSARCOMA,-PRESENT 2523 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 922 ANIMAL NUMBER: C91156 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 575.0 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:42 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 2524 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 923 ANIMAL NUMBER: C91157 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 387.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:29 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY CERVIX (CV) : -LARGE; 10 MM IN DIAMETER UTERUS (UT) : -CYST(S); MID-PORTION OF LEFT HORN, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -EDEMA,-MODERATE, FOCAL MARROW, FEMUR (FM) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CERVIX (CV) : >UNREMARKABLE UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2525 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 924 ANIMAL NUMBER: C91157 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 387.1 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:29 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) 2526 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 925 ANIMAL NUMBER: C91158 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 708.1 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 68; 110 X 90MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED FOCI, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL 2527 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 926 ANIMAL NUMBER: C91158 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 708.1 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, RED, DARK RED, GREY AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 115 X 95 X 65 MM (221.5 GRAMS), RED, GREY, TAN, YELLOW, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -MASS(ES); RIGHT, WITHIN LUMEN, EXTENDING INTO CERVIX: SINGLE MASS, 42 X 11 X 11 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -ULCERATION,-MODERATELY SEVERE, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SEVERE, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2528 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 927 ANIMAL NUMBER: C91158 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 708.1 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 7 X 5 X 2 MM (LEFT IS 5 X 2 X 2 MM) -MOTTLED; RIGHT: DARK RED AND TAN (CAP 22) LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 21) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: SINGLE DRY, RED CRUSTED FOCI ON EACH, EACH IS 3 MM IN DIAMETER; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, AXILLARY (AX) : >UNREMARKABLE FOOT/FOOTPAD(S) (FP) : -EROSION,-MINIMAL, FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL 2529 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 928 ANIMAL NUMBER: C91158 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 708.1 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SM), 2530 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 929 ANIMAL NUMBER: C91159 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 573.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 85; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT IS 4 MM IN DIAMETER) LN, MESENTERIC (MS) : -DIFFUSELY DARK; DARK GREEN PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE DARK BROWN FOCUS, 2 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 2531 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 930 ANIMAL NUMBER: C91159 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 573.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 20 X 7 X 7 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN URINARY BLADDER (UB) : -B-LEIOMYOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2532 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 931 ANIMAL NUMBER: C91159 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 573.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:33 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2533 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 932 ANIMAL NUMBER: C91160 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 253.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:55 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0360 .0349 2.0631 1.0818 1.1055 7.7748 1.0340 .0710 .0450 .3912 .0086 .0095 .6152 .0142 % .0138 % .8151 % .4274 % .4368 % 3.0718 % .4085 % .0281 % .0178 % .1546 % .0034 % .0038 % .2431 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0174 .0169 1.0000 .5244 .5358 3.7685 .5012 .0344 .0218 .1896 .0042 .0046 .2982 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK RED AREA, 2 X 1 MM GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2534 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 933 ANIMAL NUMBER: C91160 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 253.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:55 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2535 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 934 ANIMAL NUMBER: C91161 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 265.4 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 13:48 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -SMALL; 3 X 5 X 29 MM PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 13 MM SOFT, RED, DARK RED, AND TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT, MULTI FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2536 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 935 ANIMAL NUMBER: C91161 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/22/99 STUDY DAY OF DEATH: 421 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 265.4 GRAMS DATE AND TIME OF NECROPSY: 03/22/99 13:48 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL INFORMATION (XX) : >NOTE:>FINAL BODY WEIGHT WAS 265.4 GRAMS; NOT ENTERED AT TIME DEATH CODE WAS ASSIGNED THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2537 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 936 ANIMAL NUMBER: C91162 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 225.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:31 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0350 .0338 1.8884 .7299 .7633 6.5271 1.0618 .0498 .0596 .4378 .0071 .0077 .6084 .0156 % .0150 % .8393 % .3244 % .3392 % 2.9009 % .4719 % .0221 % .0265 % .1946 % .0032 % .0034 % .2704 % .0185 .0179 1.0000 .3865 .4042 3.4564 .5623 .0264 .0316 .2318 .0038 .0041 .3222 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2538 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 937 ANIMAL NUMBER: C91162 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 225.0 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:31 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2539 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 938 ANIMAL NUMBER: C91163 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 266.4 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:51 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0341 .0334 2.1433 .9469 .9284 7.0599 1.2105 .0679 .0764 .5740 .0056 .0040 .6644 .0128 % .0125 % .8045 % .3554 % .3485 % 2.6501 % .4544 % .0255 % .0287 % .2155 % .0021 % .0015 % .2494 % .0159 .0156 1.0000 .4418 .4332 3.2939 .5648 .0317 .0356 .2678 .0026 .0019 .3100 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2540 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 939 ANIMAL NUMBER: C91163 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 266.4 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 9:51 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2541 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 940 ANIMAL NUMBER: C91164 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 481.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:52 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 86; 20 X 25MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREA(S); LEFT: SINGLE DARK RED AREA, 3 X 2 MM PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -MINERALIZATION,-SLIGHT, FOCAL -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT 2542 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 941 ANIMAL NUMBER: C91164 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 481.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:52 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 35 X 20 X 15 MM, RED, DARK RED, TAN, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2543 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 942 ANIMAL NUMBER: C91164 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 659 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 481.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:52 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2544 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 943 ANIMAL NUMBER: C91165 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 228.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:45 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 6.8342 ------------------------------------------- ------------------------------- 2.9922 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2545 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 944 ANIMAL NUMBER: C91165 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 228.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:45 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2546 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 945 ANIMAL NUMBER: C91166 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:03 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS LN, MESENTERIC (MS) : -LARGE; DIFFUSE: UP TO 5 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -TELANGIECTASIS,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : >UNREMARKABLE LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL 2547 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 946 ANIMAL NUMBER: C91166 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:03 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 5 X 4 MM, RED, DARK RED, GRAY, LOBULATED, AND SOFT SKIN (SK) : >NOTE:>SECTION TAKEN FROM RIGHT SIDE DUE TO MAMMARY GLAND MASS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 57 X 40 X 23 MM, RED, TAN, LOBULATED, AND SOFT; ON SECTION EXUDES A TAN CREAMY SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION >NOTE:>SECTION TAKEN FROM RIGHT SIDE DUE TO MAMMARY GLAND MASS UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 8 X 5 X 5 MM HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2548 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 947 ANIMAL NUMBER: C91166 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 577.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:03 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SN), 2549 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 948 ANIMAL NUMBER: C91167 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 717 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:03 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 82; 40 X 45MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; PAW-HINDLEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -CALCULUS(I); BILATERAL, WITHIN PELVIS: MULTIPLE TAN AND FIRM CALCULI, UP TO 4 MM IN DIAMETER OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -HYPERPLASIA, ISLET CELLS,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL 2550 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 949 ANIMAL NUMBER: C91167 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 717 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:03 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED, TAN, AND SOFT JEJUNUM (JE) : -DIVERTICULUM(A); MID JEJUNUM: SINGLE DARK RED AND TAN AREA, 12 X 10 X 10 MM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 60 X 45 X 30 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 5 X 4 X 4 MM JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: UP TO 15 MM IN DIAMETER; COLLECTED HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : -B-ANGIOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT JOINT, OTHER (JT) : -EXOSTOSES,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL 2551 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 950 ANIMAL NUMBER: C91167 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 717 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:03 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL OBESE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2552 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 951 ANIMAL NUMBER: C91168 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYROID (TY) : -MASS(ES); INVOLVING ENTIRE LEFT THYROID: SINGLE MASS, 8 X 5 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -M-CARCINOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT 2553 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 952 ANIMAL NUMBER: C91168 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 6 X 6 MM, DARK RED, RED, TAN AND FIRM GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN MOIST BROWN PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -C-CELL CARCINOMA,-PRESENT 2554 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 953 ANIMAL NUMBER: C91168 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/05/99 STUDY DAY OF DEATH: 588 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 09/05/99 16:29 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2555 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 954 ANIMAL NUMBER: C91169 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 199.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:19 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 6.3263 ------------------------------------------- ------------------------------- 3.1695 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2556 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 955 ANIMAL NUMBER: C91169 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 199.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:19 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2557 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 956 ANIMAL NUMBER: C91170 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 258.8 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:33 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -DIFFUSELY RED; RIGHT: DARK RED -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT IS 4 X 3 X 2 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -CYST,-PRESENT 2558 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 957 ANIMAL NUMBER: C91170 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 258.8 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:33 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 7 X 5 MM, RED, DARK RED, TAN, SOFT, AND LOBULATED LN, MANDIBULAR (MN) : -LARGE; LEFT: 11 X 7 X 3 MM (RIGHT IS 4 X 3 X 2 MM ); COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER AND LOWER INCISORS -ANIMAL THIN PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, DIFFUSE 2559 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 958 ANIMAL NUMBER: C91170 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 258.8 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:33 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) (SM), 2560 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 959 ANIMAL NUMBER: C91171 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 186.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:39 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 5.8384 ------------------------------------------- ------------------------------- 3.1356 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2561 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 960 ANIMAL NUMBER: C91171 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 186.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:39 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2562 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 961 ANIMAL NUMBER: C91172 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 456.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:59 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 5 X 4 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY EYE (EY) : -ULCERATION, CORNEAL,-MINIMAL, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -HYPERPLASIA, ACINAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL 2563 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 962 ANIMAL NUMBER: C91172 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 456.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:59 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: SINGLE RED AND TAN CRUSTED AREA ON EACH, EACH IS 3 MM IN DIAMETER; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2564 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 963 ANIMAL NUMBER: C91172 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 456.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:59 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2565 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 964 ANIMAL NUMBER: C91173 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/05/99 STUDY DAY OF DEATH: 404 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/99 10:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, DARK RED AND TAN MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 20) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, FOCAL SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL 2566 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 965 ANIMAL NUMBER: C91173 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/05/99 STUDY DAY OF DEATH: 404 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/99 10:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED -DISCHARGE-NASAL; BILATERAL: DARK RED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2567 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 966 ANIMAL NUMBER: C91173 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/05/99 STUDY DAY OF DEATH: 404 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/05/99 10:23 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2568 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 967 ANIMAL NUMBER: C91174 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 256.6 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:05 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0350 .0298 2.0196 1.0395 .9432 7.3862 1.2895 .0904 .0654 .4707 .0067 .0060 .5262 .0136 % .0116 % .7871 % .4051 % .3676 % 2.8785 % .5025 % .0352 % .0255 % .1834 % .0026 % .0023 % .2051 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0173 .0148 1.0000 .5147 .4670 3.6573 .6385 .0448 .0324 .2331 .0033 .0030 .2605 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OVARY (OV) : -CYST(S); LEFT: SINGLE RED FLUID FILLED CYST, 8 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -HEMORRHAGE,-MODERATE, FOCAL OVARY (OV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2569 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 968 ANIMAL NUMBER: C91174 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 256.6 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:05 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW,STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS) 2570 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 969 ANIMAL NUMBER: C91175 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 74; 50 X 45MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: TAN GRANULAR MATERIAL PITUITARY (PI) : -MASS(ES); 10 7 X 6 MM, RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: MULTIPLE MASSES, UP TO 40 X 35 X 25 MM, ALL ARE RED, DARK RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN SEMIFLUID; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 2571 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 970 ANIMAL NUMBER: C91175 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2572 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 971 ANIMAL NUMBER: C91175 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 514.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2573 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 972 ANIMAL NUMBER: C91176 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 445.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:10 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; PAW-HINDLEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -ADHESION(S); RIGHT LATERAL LOBE TO RIGHT MEDIAN LOBE: MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 6 X 5 MM (RIGHT IS 4 X 3 X 2 MM) PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FIBROSIS, CAPSULAR,-MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATELY SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2574 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 973 ANIMAL NUMBER: C91176 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 445.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:10 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE BROWN ERODED AREA, 4 X 2 MM -THICKENED WALL; DIFFUSE SKIN (SK) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING MAMMARY, FEMALE (MF) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING UTERUS (UT) : -MASS(ES); SEROSAL SURFACE OF LEFT HORN: SINGLE MASS, 4 X 3 X 3 MM, TAN AND FIRM; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: 20 X 9 X 8 MM (RIGHT IS 10 X 6 X 5 MM); COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, GL (ST) : -EDEMA,-MODERATELY SEVERE, FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL SKIN (SK) : >TISSUE MISSING MAMMARY, FEMALE (MF) : >TISSUE MISSING UTERUS (UT) : -B-LEIOMYOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION JOINT, OTHER (JT) : -EXOSTOSES,-SLIGHT, MULTI-FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL 2575 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 974 ANIMAL NUMBER: C91176 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 445.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:10 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 2576 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 975 ANIMAL NUMBER: C91177 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 626.3 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 63; 130 X 110MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 145 X 110 X 50 MM (274.3 GRAMS), TAN, RED, DARK RED, VASCULAR, LOBULATED AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 11 X 6 X 3 MM (RIGHT IS UP TO 7 X 4 X 2 MM) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT SKIN (SK) : -FAT NECROSIS,-MODERATE, MULTI-FOCAL -B-LIPOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : >UNREMARKABLE CAUSE OF DEATH (CD) : -LIPOMA,-PRESENT LN, AXILLARY (AX) : >UNREMARKABLE 2577 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 976 ANIMAL NUMBER: C91177 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 626.3 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:10 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2578 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 977 ANIMAL NUMBER: C91178 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 370.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:53 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); VENTRAL-ABDOMINAL; WEEK 90; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED PITUITARY (PI) : -MASS(ES); 10 X 8 7 MM, RED, DARK RED, TAN, LOBULATED, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2579 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 978 ANIMAL NUMBER: C91178 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 370.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:53 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION 2580 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 979 ANIMAL NUMBER: C91178 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 370.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 13:53 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2581 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 980 ANIMAL NUMBER: C91179 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/98 STUDY DAY OF DEATH: 324 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: 370.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/98 12:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 36; 65 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 38; 80 X 65MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC,-MODERATE, DIFFUSE HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 2582 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 981 ANIMAL NUMBER: C91179 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/98 STUDY DAY OF DEATH: 324 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: 370.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/98 12:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN AREAS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 75 X 63 X 45 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -CYST(S); VENTRAL CERVICAL REGION: MULTIPLE TAN AND CREAMY SEMI-FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER (CAP 22) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 60 X 60 X 20 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -M-FIBROSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT 2583 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 982 ANIMAL NUMBER: C91179 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/98 STUDY DAY OF DEATH: 324 STUDY WEEK OF DEATH: 47 TERMINAL BODY WEIGHT: 370.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/98 12:14 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2584 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 983 ANIMAL NUMBER: C91180 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/16/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/16/99 13:36 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 32; 150 X 120MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE BROWN LINEAR AREA, 3 X LESS THAN 1 MM -LARGE; 7 X 7 X 5 MM, APPEARS SWOLLEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-SLIGHT, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2585 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 984 ANIMAL NUMBER: C91180 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/16/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/16/99 13:36 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 120 X 90 X 40 MM (242.0 GRAMS), RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE INGUINAL) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION LN, INGUINAL (IN) : -LARGE; RIGHT: 10 X 5 X 2 MM (FOR COMPARISON, LEFT IS 5 X 2 X 1 MM) (CAP 20) -DIFFUSELY RED; BILATERAL HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, DIFFUSE -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL 2586 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 985 ANIMAL NUMBER: C91180 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/16/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/16/99 13:36 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2587 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 986 ANIMAL NUMBER: C91181 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 302.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:31 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0283 .0357 1.9333 1.0638 1.0540 9.3987 1.2459 .0782 .0859 .4567 .0079 .0102 .5781 .0093 % .0118 % .6385 % .3513 % .3481 % 3.1039 % .4115 % .0258 % .0284 % .1508 % .0026 % .0034 % .1909 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0146 .0185 1.0000 .5503 .5452 4.8615 .6444 .0404 .0444 .2362 .0041 .0053 .2990 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2588 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 987 ANIMAL NUMBER: C91181 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 302.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 11:31 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2589 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 988 ANIMAL NUMBER: C91182 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 316.1 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 87; 10 X 15MM; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREA(S); BILATERAL: MULTIPLE DARK RED, PINPOINT FOCI, UP TO 4 X 2 MM PITUITARY (PI) : -MASS(ES); 15 X 9 X 7 MM, RED, DARK RED, TAN, SOFT AND LOBULATED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL, MULTI-FOCAL 2590 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 989 ANIMAL NUMBER: C91182 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 316.1 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 20 X 17 X 8 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -ANIMAL THIN MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2591 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 990 ANIMAL NUMBER: C91182 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 316.1 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:35 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2592 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 991 ANIMAL NUMBER: C91183 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:15 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); LEFT PITUITARY (PI) : -MASS(ES); 9 X 5 X 5 MM, DARK RED, TAN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2593 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 992 ANIMAL NUMBER: C91183 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:15 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION ADIPOSE TISSUE (AT) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE NEAR URINARY BLADDER: SINGLE MASS, 22 X 12 X 10 MM, RED, DARK RED, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -M-CARCINOMA, TRANSITIONAL CELL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT ADIPOSE TISSUE (AT) : -I-TRANSITIONAL CELL CARCINOMA,NEOPLASM PRESENT 2594 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 993 ANIMAL NUMBER: C91183 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.5 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:15 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 2595 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 994 ANIMAL NUMBER: C91184 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 629 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 456.9 GRAMS DATE AND TIME OF NECROPSY: 10/16/99 7:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN KIDNEY (KD) : -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 MM IN DIAMETER (RIGHT IS 4 MM IN DIAMETER) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2596 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 995 ANIMAL NUMBER: C91184 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 629 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 456.9 GRAMS DATE AND TIME OF NECROPSY: 10/16/99 7:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 9 X 8 MM, RED, DARK RED AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE DARK BROWN ERODED AREAS, UP TO 3 X 2 MM CECUM (CE) : -SMALL; 30 X 6 X 6 MM MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTIONS UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MUCOID SEMIFLUID MATERIAL FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DRY, DARK RED CRUSTED AREA, 10 X 8 MM; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY, YELLOW MATERIAL -BLOOD FILM TAKEN PERINEUM: PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE CECUM (CE) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-SLIGHT, DIFFUSE UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL VAGINA (VA) : ^UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL 2597 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 996 ANIMAL NUMBER: C91184 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 629 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 456.9 GRAMS DATE AND TIME OF NECROPSY: 10/16/99 7:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 2598 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 997 ANIMAL NUMBER: C91185 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 217.6 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:14 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -MOTTLED; BILATERAL: BROWN AND DARK BROWN PITUITARY (PI) : -MASS(ES); 13 X 10 X 10 MM, RED, DARK RED, GRAY, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE WHITE, ERODED AREAS, UP TO 4 X 3 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE, MULTI FOCAL OVARY (OV) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -ONE EXAMINED,-PRESENT STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : >UNREMARKABLE 2599 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 998 ANIMAL NUMBER: C91185 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 217.6 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:14 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2600 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 999 ANIMAL NUMBER: C91185 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 217.6 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:14 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2601 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1000 ANIMAL NUMBER: C91186 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 488.8 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 76; 90 X 80MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 4 X 4 X 3 MM) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2602 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1001 ANIMAL NUMBER: C91186 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 488.8 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT ABDOMINAL REGION: SINGLE MASS, 90 X 70 X 55 MM (205.9 GRAMS), TAN, RED, SOFT AND VASCULAR; SAME ON CUT SURFACE; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); VENTRAL THORACIC REGION AND RIGHT INGUINAL REGION: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 X 10 X 10 MM (CAPS 21 AND 22) LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL OVARY (OV) : -ONE EXAMINED,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CECUM (CE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 2603 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1002 ANIMAL NUMBER: C91186 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 488.8 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND SOFT (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT 2604 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1003 ANIMAL NUMBER: C91186 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 488.8 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2605 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1004 ANIMAL NUMBER: C91187 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 257.8 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 71; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL OVARY (OV) : -MINERALIZATION,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2606 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1005 ANIMAL NUMBER: C91187 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 257.8 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 MM IN DIAMETER, RED, DARK RED, TAN, VASCULAR AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 30 X 25 X 25 MM, RED, DARK RED, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -MOTTLED; LEFT: DARK RED AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -ANIMAL THIN STOMACH, GL (ST) : -EDEMA,-MINIMAL, DIFFUSE STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2607 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1006 ANIMAL NUMBER: C91187 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 257.8 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2608 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1007 ANIMAL NUMBER: C91188 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:27 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 88; 65 X 70MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 101; 60 X 55MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 74; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE BLACK AREA, 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); PERINEAL REGION: SINGLE MASS, 30 X 21 X 15 MM, TAN, DARK BROWN, VASCULAR AND FIRM; ON CUT SECTION EXUDES A TAN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 20) HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -HEMORRHAGE,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL PARATHYROID (PT) : -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : ^UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -NECROSIS,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 2609 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1008 ANIMAL NUMBER: C91188 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:27 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); TRIMMING COMMENT: ON ROUTINE SECTION: SINGLE TAN, CREAMY SEMISOLID FILLED CYST, 11 X 9 MM; TRIMMED ON ROUTINE SECTION -MASS #2; LEFT LATERAL ABDOMINAL REGION: SINGLE MASS, 70 X 57 X 40 MM, RED, TAN, VASCULAR, AND FIRM; ON CUT SECTION EXUDES A RED FLUID AND A TAN CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NORMAL -THICKENED; ALL REGIONS, DIFFUSE: TAN, BROWN, AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; MID VENTRAL CERVICAL EXTENDING TO MID THORACIC REGION: SINGLE MASS, 108 X 70 X 36 MM, RED, TAN, VASCULAR, LOBULATED, AND SOFT; ON SECTION EXUDES A TAN TENACIOUS SEMISOLID; PORTION COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) UTERUS (UT) : -DILATATION,-SLIGHT, DIFFUSE VAGINA (VA) : -B-LEIOMYOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2610 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1009 ANIMAL NUMBER: C91188 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:27 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, INGUINAL (IN) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL 2611 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1010 ANIMAL NUMBER: C91188 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:27 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) 2612 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1011 ANIMAL NUMBER: C91189 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 313.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 12:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; LIMBS-HIND; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 88; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -THROMBUS,-SLIGHT, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT 2613 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1012 ANIMAL NUMBER: C91189 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 313.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 12:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 35 X 31 X 12 MM, RED, TAN, GREY, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE MOIST, TAN ULCERATED AREA, 20 X 19 X 6 MM; COLLECTED WITH MASS LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 7 X 5 X 5 MM (CAP 21) FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE MOIST, TAN ABRADED AREA, 17 X 14 MM; COLLECTED >NOTE:>SORE/SCAB (RIGHT HINDFOOT) NOTED IN CLINICAL OBSERVATIONS NOT SEEN AT NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : >UNREMARKABLE FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2614 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1013 ANIMAL NUMBER: C91189 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 313.1 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 12:59 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2615 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1014 ANIMAL NUMBER: C91190 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:37 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 100; 50 X 45MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL ADRENAL, CORTEX (AC) : -DIFFUSELY DARK; BILATERAL: DARK BROWN THYROID (TY) : -LARGE; LEFT: 7 X 4 X 4 MM (RIGHT IS 6 X 3 X 2 MM) -DIFFUSELY DARK; LEFT: DARK BROWN PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -THROMBUS,-MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL 2616 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1015 ANIMAL NUMBER: C91190 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:37 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION, EXTENDING TO MID THORACIC REGION: SINGLE MASS, 55 X 35 X 20 MM, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE MANDIBULAR) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2617 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1016 ANIMAL NUMBER: C91190 SEX: FEMALE DOSE GROUP: 1 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:37 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2618 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-212 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e V II o f X II Page 2619 of 4873 Covance 6329-212 3M T-6316.1 VOLUME VII OF XII CONTENTS Page A P P E N D I X 6 ( C o n t i n u e d ) .................................................................................................................................................... 2 6 2 1 I n d i v i d u a l A n a t o m i c P a t h o l o g y D a t a .................................................................................................................... 2 6 2 2 2620 APPENDIX 6 (Continued) In d iv id u a l A n a to m ic P a th o lo g y D a ta Covance 6329-212 3M T-6316.1 2621 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1017 ANIMAL NUMBER: C91191 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/06/99 8:04 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 83; 70 X 70MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; LIMB-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 92; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN -DEPRESSED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 20 X 12 MM; COLLECTED ON ROUTINE SECTION LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, DIFFUSE KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL SPLEEN (SP) : -FIBROSIS, CAPSULAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : >UNREMARKABLE PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2622 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1018 ANIMAL NUMBER: C91191 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/06/99 8:04 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: TWO MASSES, EACH IS 18 X 18 X 5 MM, BOTH ARE TAN AND SOFT; SAME ON BOTH CUT SURFACES; BOTH COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -MASS #2; VENTRAL ABDOMINAL REGION, EXTENDING TO BILATERAL INGUINAL REGIONS: SINGLE MASS, 75 X 65 X 20 MM, TAN, RED, FIRM, LOBULATED AND VASCULAR; SAME ON CUT SURFACES; COLLECTED INTACT; REGIONAL LN (RIGHT INGUINAL) NORMAL (CAP 22) (LEFT INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND SOFT; COLLECTED ON ROUTINE SECTION LN, AXILLARY (AX) : -DIFFUSELY RED; RIGHT: DIFFUSELY DARK (CAP 21) STOMACH, GL (ST) : -EROSION,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -NECROSIS,-MODERATE, FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATE, FOCAL 2623 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1019 ANIMAL NUMBER: C91191 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/06/99 8:04 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DRY BROWN ULCERATED AREA, 10 MM IN DIAMETER; SURROUNDING TISSUE APPEARS SWOLLEN; COLLECTED GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS FOOT/FOOTPAD(S) (FP) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, DIFFUSE 2624 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1020 ANIMAL NUMBER: C91191 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/06/99 8:04 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2625 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1021 ANIMAL NUMBER: C91192 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 243.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:51 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0329 .0300 1.9280 .8220 .7920 6.3344 1.1493 .0567 .0586 .4607 .0056 .0043 .5438 .0135 % .0123 % .7908 % .3372 % .3249 % 2.5982 % .4714 % .0233 % .0240 % .1890 % .0023 % .0018 % .2231 % .0171 .0156 1.0000 .4263 .4108 3.2855 .5961 .0294 .0304 .2390 .0029 .0022 .2821 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MANDIBULAR (MN) : -RED FOCUS(I)/AREA(S); BILATERAL: MULTIPLE DARK RED PINPOINT FOCI (CAP 20) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2626 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1022 ANIMAL NUMBER: C91192 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 243.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:51 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2627 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1023 ANIMAL NUMBER: C91193 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 306.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 14:23 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 85; 25 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, RED, GRAY AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 40 X 30 X 18 MM, RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A YELLOW CREAMY SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) CERVIX (CV) : -LARGE; UP TO 7 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CERVIX (CV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2628 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1024 ANIMAL NUMBER: C91193 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 306.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 14:23 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HIND FOOT: SINGLE DARK RED ABRADED AREA, 3 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; RED AND YELLOW MOIST MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL FOOT/FOOTPAD(S) (FP) : -CYST, EPIDERMAL INCLUSION,-PRESENT -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL 2629 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1025 ANIMAL NUMBER: C91193 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 306.8 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 14:23 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2630 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1026 ANIMAL NUMBER: C91194 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/23/00 STUDY DAY OF DEATH: 728 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 356.1 GRAMS DATE AND TIME OF NECROPSY: 01/23/00 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 71; 65 X 60MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 10 X 4 MM, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 2631 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1027 ANIMAL NUMBER: C91194 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/23/00 STUDY DAY OF DEATH: 728 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 356.1 GRAMS DATE AND TIME OF NECROPSY: 01/23/00 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 61 X 52 X 20 MM, RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 29 X 20 X 19 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL 2632 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1028 ANIMAL NUMBER: C91194 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/23/00 STUDY DAY OF DEATH: 728 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 356.1 GRAMS DATE AND TIME OF NECROPSY: 01/23/00 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2633 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1029 ANIMAL NUMBER: C91195 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 366.3 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 13:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 87; 45 X 35MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 12 X 10 X 7 MM, DARK RED, RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL SPLEEN (SP) : -B-HEMANGIOMA,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2634 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1030 ANIMAL NUMBER: C91195 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 366.3 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 13:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY JEJUNUM (JE) : -DIVERTICULUM(A); TRIMMING COMMENT: MID JEJUMUM: SINGLE AREA, 6 X 5 X 5 MM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 40 X 35 X 20 MM, TAN, GRAY, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 4 X 3 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, MULTI-FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL JEJUNUM (JE) : -DIVERTICULUM,-PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2635 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1031 ANIMAL NUMBER: C91195 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 366.3 GRAMS DATE AND TIME OF NECROPSY: 12/20/99 13:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2636 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1032 ANIMAL NUMBER: C91196 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 360.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:04 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 80; 50 X 30MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; EARS; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 9 X 9 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, DIFFUSE -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 2637 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1033 ANIMAL NUMBER: C91196 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 360.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:04 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 38 X 26 X 23 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION FOOT/FOOTPAD(S) (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE TAN AND DRY ABRADED AREA, 6 X 5 MM, COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL 2638 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1034 ANIMAL NUMBER: C91196 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 360.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:04 PROSECTOR: TONY HANFELD RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2639 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1035 ANIMAL NUMBER: C91197 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:14 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 MM IN DIAMETER (LEFT IS 7 X 4 X 3 MM); APPEARS CYSTIC PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 4 X 2 MM JEJUNUM (JE) : -LARGE LYMPHOID AREA(S); DIFFUSE: UP TO 7 X 4 X 3 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -CYST,-SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : -HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE),-MINIMAL, MULTI-FOCAL 2640 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1036 ANIMAL NUMBER: C91197 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:14 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY ILEUM (IL) : -LARGE LYMPHOID AREA(S); DIFFUSE: UP TO 7 X 4 X 3 MM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 7 MM IN DIAMETER, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) -THICKENED; LEFT INGUINAL REGION: TAN, DARK BROWN AND SOFT; EXUDES A DARK BROWN FLUID; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); RIGHT, WITHIN LUMEN: SINGLE CLEAR FLUID FILLED CYST, 6 X 3 X 3 MM -MASS(ES); DISTAL END OF RIGHT HORN, WITHIN LUMEN: SINGLE MASS, 22 X 5 X 5 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN ILEUM (IL) : -HYPERPLASIA, GALT (GUT ASSOCIATED LYMPHOID TISSUE),-MINIMAL, MULTI-FOCAL MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL 2641 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1037 ANIMAL NUMBER: C91197 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 583.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:14 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) 2642 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1038 ANIMAL NUMBER: C91198 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 231.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:11 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 7.5213 ------------------------------------------- ------------------------------- 3.2532 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2643 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1039 ANIMAL NUMBER: C91198 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 231.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:11 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2644 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1040 ANIMAL NUMBER: C91199 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 371.8 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: JERALD W. JOHNSON RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; CIRCLING; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 7 MM IN DIAMETER; SOME APPEAR SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT IS 5 MM IN DIAMETER) -RED FOCUS(I)/AREA(S); RIGHT: SINGLE DARK RED AREA, 6 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -THROMBUS,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2645 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1041 ANIMAL NUMBER: C91199 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 371.8 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: JERALD W. JOHNSON RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, RED, DARK RED, TAN, GRAY AND SOFT MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2646 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1042 ANIMAL NUMBER: C91199 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 371.8 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: JERALD W. JOHNSON RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2647 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1043 ANIMAL NUMBER: C91200 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 445.0 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 13:57 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); HEAD; WEEK 82; 30 X 35MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; HEADCRANIAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -MASS(ES); TOP OF HEAD, EXTENDS TO BOTH AUDITORY CANALS AND ORBITS: SINGLE SUBCUTANEOUS MASS, 23 X 35 X 52 MM, FIRM, RED, TAN AND SMOOTH; SAME ON CUT SURFACE; OVERLYING SKIN HAS A DARK BROWN, DRY CRUSTED AREA, 10 X 20 MM (COLLECTED INTACT WITH MASS); REGIONAL LN (MANDIBULAR) NORMAL (COLLECTED) HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT 2648 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1044 ANIMAL NUMBER: C91200 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 445.0 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 13:57 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CALVARIUM/SKULL (CA) : -DEPRESSED AREA(S); ENTIRE SKULL CAP UNDER SKIN MASS (COLLECTED) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -FIBROSARCOMA,-PRESENT CALVARIUM/SKULL (CA) : ^UNREMARKABLE 2649 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1045 ANIMAL NUMBER: C91200 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 445.0 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 13:57 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) 2650 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1046 ANIMAL NUMBER: C91201 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:46 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 74; 60 X 45MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 97; 50 X 30MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 105; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MASS(ES); ATTACHED TO MARGIN OF LEFT LATERAL LOBE: SINGLE MASS, 15 X 15 X 4 MM, DARK RED, BROWN AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (HEPATIC) NOT IDENTIFIED PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, TAN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 2651 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1047 ANIMAL NUMBER: C91201 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:46 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 65 X 45 X 43 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SOME SECTIONS EXUDE A TAN, CREAMY MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; VENTRAL LUMBAR REGION: SINGLE MASS, 55 X 35 X 28 MM, DARK BROWN, TAN AND CYSTIC; ON SECTION EXUDES A DARK BROWN FLUID; COLLECTED; POSSIBLY CONSISTENT WITH MASSES (INGUINAL REGION, RIGHT AND VENTRAL ABDOMINAL REGION, RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; REGIONAL LN (LUMBAR) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2652 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1048 ANIMAL NUMBER: C91201 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 12:46 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX) 2653 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1049 ANIMAL NUMBER: C91202 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 619.0 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:40 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 76; 40 X40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK 84; 10 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRALTHORACIC-RIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 84; 20 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 5 X 5 MM (RIGHT IS 6 X 4 X 3 MM) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL 2654 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1050 ANIMAL NUMBER: C91202 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 619.0 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:40 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY AND RIGHT VENTRAL THORACIC REGIONS: TWO MASSES, RIGHT AXILLARY IS 50 X 34 X 23 MM, RIGHT THORACIC IS 23 X 23 X 10 MM; BOTH ARE TAN, RED, DARK RED, FIRM, AND LOBULATED; SAME ON CUT SURFACES; OVERLYING SKIN ON THE THORACIC REGION MASS HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 19 MM IN DIAMETER; MASS WITH CRUSTED AREA COLLECTED; REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE CREAMY FLUID FILLED CYSTS, UP TO 10 MM IN DIAMETER (CAP 22) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 27 X 19 X 14 MM, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, AXILLARY (AX) : -MOTTLED; RIGHT: DARK RED AND TAN (LEFT NOT IDENTIFIED) (CAP 21) SKIN (SK) : -ULCERATION,-SEVERE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2655 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1051 ANIMAL NUMBER: C91202 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 619.0 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:40 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2656 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1052 ANIMAL NUMBER: C91203 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/11/99 STUDY DAY OF DEATH: 532 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/11/99 14:16 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 60; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES; MARGINS APPEAR ROUNDED ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED -LARGE; BILATERAL: EACH IS 6 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 7 X 5 X 5 MM, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2657 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1053 ANIMAL NUMBER: C91203 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/11/99 STUDY DAY OF DEATH: 532 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/11/99 14:16 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN, ERODED AREA, 2 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 40 X 35 X 35 MM, TAN, RED, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A TAN, CREAMY SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN STOMACH, NONGL (SU) : -EDEMA,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION 2658 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1054 ANIMAL NUMBER: C91203 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/11/99 STUDY DAY OF DEATH: 532 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/11/99 14:16 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2659 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1055 ANIMAL NUMBER: C91204 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 426 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 8:29 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 35; 45 X 35MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -LARGE; 7 X 5 X 4 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL ABDOMINAL REGION: SINGLE MASS, 65 X 55 X 18 MM, RED, DARK RED, BLACK, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE INGUINAL); OVERLYING SKIN HAS A SINGLE BLACK AND MOIST ULCERATED AREA, 15 MM IN DIAMETER; COLLECTED WITH MASS HISTOPATHOLOGY EYE (EY) : -MINERALIZATION, CORNEAL,-MODERATE, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FIBROSIS,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-SLIGHT, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2660 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1056 ANIMAL NUMBER: C91204 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 426 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 8:29 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, INGUINAL (IN) : -LARGE; LEFT: 15 X 13 X 10 MM (FOR COMPARISON, RIGHT IS 4 X 2 X 2 MM) (CAP 20) -DIFFUSELY RED; LEFT: DARK RED (CAP 20) GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: CLEAR MOIST MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : >UNREMARKABLE 2661 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1057 ANIMAL NUMBER: C91204 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 426 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 8:29 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2662 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1058 ANIMAL NUMBER: C91205 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 167.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:19 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 5.6721 ------------------------------------------- ------------------------------- 3.3944 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2663 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1059 ANIMAL NUMBER: C91205 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 167.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:19 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2664 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1060 ANIMAL NUMBER: C91206 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 336.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:05 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 6 MM, DARK RED, TAN, GRAY, LOBULATED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL VAGINA (VA) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT 2665 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1061 ANIMAL NUMBER: C91206 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 336.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:05 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 12 X 8 X 6 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER AND LOWER INCISORS CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, DIFFUSE 2666 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1062 ANIMAL NUMBER: C91206 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 336.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:05 PROSECTOR: KEVIN BILLINGS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2667 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1063 ANIMAL NUMBER: C91207 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY OVARY (OV) : -CYST(S); RIGHT: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2668 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1064 ANIMAL NUMBER: C91207 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), KIDNEY (KD), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) 2669 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1065 ANIMAL NUMBER: C91208 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 259.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:30 PROSECTOR: JODI THIEREN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LIGHT FOCUS(I)/a REA(S); BILATERAL: MULTIPLE TAN PINPOINT FOCI OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 12 X 9 X 9 MM, DARK RED, TAN, LOBULATED AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT CAVITY, ABDOM (PC) : -NECROSIS, FAT,-PRESENT 2670 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1066 ANIMAL NUMBER: C91208 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 259.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:30 PROSECTOR: JODI THIEREN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, NEAR RIGHT OVARY: SINGLE MASS, 6 X 5 X 5 MM, TAN, YELLOW, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (RENAL) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -ANIMAL THIN 2671 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1067 ANIMAL NUMBER: C91208 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 519 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 259.8 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 15:30 PROSECTOR: JODI THIEREN RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, RENAL (RL) 2672 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1068 ANIMAL NUMBER: C91209 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/29/99 15:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, DARK RED AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : >UNREMARKABLE THYMUS (TH) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 2673 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1069 ANIMAL NUMBER: C91209 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/29/99 STUDY DAY OF DEATH: 520 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/29/99 15:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2674 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1070 ANIMAL NUMBER: C91210 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:11 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADCRANIAL; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, GRAY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -TELANGIECTASIS,-MODERATE, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2675 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1071 ANIMAL NUMBER: C91210 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:11 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); DIFFUSE: MULTIPLE TAN AND CREAMY FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 7 X 5 X 5 MM LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE 2676 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1072 ANIMAL NUMBER: C91210 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:11 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2677 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1073 ANIMAL NUMBER: C91211 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 562.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:04 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 86; 20 X 40MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -RED FOCUS(I)/AREA(S); LEFT AND RIGHT LATERAL LOBES: MULTIPLE RED AREAS UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : >NOTE:>LEFT ADRENAL DAMAGED AT NECROPSY PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, DARK RED, GRAY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2678 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1074 ANIMAL NUMBER: C91211 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 562.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:04 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 36 X 24 X 20 MM, DARK RED, RED, GRAY, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN OF MASS HAS A BLACK CRUSTED AREA, 10 MM IN DIAMETER; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -ANIMAL OBESE GENERAL INFORMATION (XX) : >NOTE:>NECROPSIED OUT OF ORDER DUE TO MORIBUND CONDITION FOLLOWING BLOOD COLLECTION CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2679 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1075 ANIMAL NUMBER: C91211 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 562.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:04 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), THYROID (TY), SKIN (SK), URINARY BLADDER (UB) 2680 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1076 ANIMAL NUMBER: C91212 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/99 13:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 49; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 6 MM IN DIAMETER PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 3 X 2 MM -LARGE; 6 X 6 X 3 MM; APPEARS SWOLLEN HISTOPATHOLOGY BRAIN (BR) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-SEVERE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2681 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1077 ANIMAL NUMBER: C91212 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/99 13:20 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2682 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1078 ANIMAL NUMBER: C91213 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 339.7 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 16:14 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 11 X 10 X 10 MM (FOR COMPARISON, RIGHT IS 6 X 5 X 4 MM) PITUITARY (PI) : -MASS(ES); 10 X 10 X 7 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FIBROSIS,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE 2683 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1079 ANIMAL NUMBER: C91213 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 339.7 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 16:14 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN AND CREAMY SEMIFLUID GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2684 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1080 ANIMAL NUMBER: C91213 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 535 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 339.7 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 16:14 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2685 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1081 ANIMAL NUMBER: C91214 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 557.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 15:17 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 64; 50 X 90MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 95; 85 X 90MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 84; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -SMALL; LEFT: 3 MM IN DIAMETER (RIGHT IS 5 MM IN DIAMETER) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL THYROID (TY) : -HEMORRHAGE,-MINIMAL, FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2686 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1082 ANIMAL NUMBER: C91214 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 557.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 15:17 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); RIGHT POSTERIOR ASPECT: SINGLE MASS, 4 X 3 X 2 MM, RED, GREY, BROWN AND SOFT -LARGE; 7 X 5 X 5 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 19 X 18 X 11 MM, GREY, TAN, AND SOFT; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 91 X 53 X 35 MM, RED, DARK RED, GREY, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL-LEFT- ABDOMINAL REGION) AS NOTED IN CLINICAL OBSERVATIONS -MASS #3; INVOLVING ENTIRE VENTRAL CERVICAL AND THORACIC REGIONS: SINGLE MASS, 101 X 97 X 58 MM (141.7 GRAMS), RED, DARK RED, GREY, TAN, LOBULATED, VASCULAR AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE MANDIBULAR) PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT 2687 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1083 ANIMAL NUMBER: C91214 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 557.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 15:17 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 6 X 5 X 3 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -ANIMAL THIN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, MANDIBULAR (MN) : >TISSUE MISSING 2688 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1084 ANIMAL NUMBER: C91214 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 557.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 15:17 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SM), 2689 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1085 ANIMAL NUMBER: C91215 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/99 STUDY DAY OF DEATH: 378 STUDY WEEK OF DEATH: 54 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 12:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >HOUSING,FOOD,WATER & MAINT.; TEETH CUT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; LIMBSHIND; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 15 X 12 MM, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 2690 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1086 ANIMAL NUMBER: C91215 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/99 STUDY DAY OF DEATH: 378 STUDY WEEK OF DEATH: 54 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 12:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY UTERUS (UT) : -DILATATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2691 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1087 ANIMAL NUMBER: C91215 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/99 STUDY DAY OF DEATH: 378 STUDY WEEK OF DEATH: 54 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 12:55 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2692 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1088 ANIMAL NUMBER: C91216 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 98; 50 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE LEFT GLAND: SINGLE MASS, 18 X 13 X 10 MM, DARK RED, TAN AND LOBULATED; ON SECTION, EXUDES A TAN CASEOUS MATERIAL; REGIONAL LN (SEE RENAL) OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR FLUID FILLED CYSTS ON EACH, EACH IS UP TO 5 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -CYST, BILIARY,-MODERATE, FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -M-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT 2693 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1089 ANIMAL NUMBER: C91216 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ANTERIOR ASPECT: SINGLE DARK RED AREA, 3 MM IN DIAMETER -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 30 X 25 X 20 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 23) LN, RENAL (RL) : -LARGE; RIGHT: 7 X 4 X 2 MM (LEFT IS 3 X 2 X 1 MM) (CAP 22) -DIFFUSELY RED; RIGHT: DARK RED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LUNG (LU) : -N-MALIGNANT PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2694 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1090 ANIMAL NUMBER: C91216 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 365.8 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), URINARY BLADDER (UB), LN, INGUINAL (IN) 2695 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1091 ANIMAL NUMBER: C91217 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/12/99 STUDY DAY OF DEATH: 472 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 271.5 GRAMS DATE AND TIME OF NECROPSY: 05/12/99 13:45 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); TRIMMING COMMENT: RIGHT OVARY (OV) : -CYST(S); TRIMMING COMMENT: RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MODERATE, FOCAL -DILATATION, PELVIC,-PRESENT -HEMORRHAGE,-SLIGHT, FOCAL -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL, MULTI-FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2696 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1092 ANIMAL NUMBER: C91217 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/12/99 STUDY DAY OF DEATH: 472 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 271.5 GRAMS DATE AND TIME OF NECROPSY: 05/12/99 13:45 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, DARK RED, AND SOFT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -ANIMAL THIN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2697 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1093 ANIMAL NUMBER: C91217 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/12/99 STUDY DAY OF DEATH: 472 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 271.5 GRAMS DATE AND TIME OF NECROPSY: 05/12/99 13:45 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2698 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1094 ANIMAL NUMBER: C91218 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 329.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:41 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; PAPILLARY PROCESS: TAN (CAP 21) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT 2699 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1095 ANIMAL NUMBER: C91218 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 329.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:41 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 10 X 10 MM, DARK RED, GRAY AND FIRM STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, DIFFUSE -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CERVIX (CV) : -DILATATION,-SLIGHT, FOCAL -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2700 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1096 ANIMAL NUMBER: C91218 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 329.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:41 PROSECTOR: JODI THIEREN RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (MS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) 2701 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1097 ANIMAL NUMBER: C91219 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 252.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:00 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); VENTRAL-ABDOMINAL; WEEK 88; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 7 X 7 MM, RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 18 X 13 X 8 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2702 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1098 ANIMAL NUMBER: C91219 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 252.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:00 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; MUCOSA: GREEN MUCOID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; DARK RED, DRY MATERIAL -DISCHARGE-NASAL; DARK RED, DRY MATERIAL -MALOCCLUSION; BILATERAL UPPER INCISORS -ANIMAL THIN VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2703 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1099 ANIMAL NUMBER: C91219 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 252.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 12:00 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2704 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1100 ANIMAL NUMBER: C91220 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 622.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 82; 70 X 70MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 82; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 84; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 97; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -MASS(ES); WITHIN PANCREAS, NEAR SPLEEN: SINGLE MASS, 14 X 10 X 8 MM, RED, DARK RED, TAN, LOBULATED, FIRM, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED INTACT WITH PANCREAS; REGIONAL LN (SEE ANTERIOR MESENTERIC) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL PANCREAS (PA) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2705 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1101 ANIMAL NUMBER: C91220 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 622.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, DARK RED, TAN, LOBULATED, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 40 X 35 X 25 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 35 X 30 X 20 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 23 MM IN DIAMETER, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 70 X 70 X 50 MM, RED, DARK RED, TAN, FIRM, LOBULATED, AND VASCULAR; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED PITUITARY (PI) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATE, FOCAL -B-FIBROADENOMA,- 4 NEOPLASMS PRESENT UTERUS (UT) : -DILATATION,-SEVERE, DIFFUSE 2706 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1102 ANIMAL NUMBER: C91220 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 622.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -CYST(S); WITHIN WALLS OF LEFT HORN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; COLLECTED LN, ANT MES/PANC (AP) : -DIFFUSELY RED; (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL LN, ANT MES/PANC (AP) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2707 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1103 ANIMAL NUMBER: C91220 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 622.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:40 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB), LN, AXILLARY (AX) 2708 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1104 ANIMAL NUMBER: C91221 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 591.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 2709 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1105 ANIMAL NUMBER: C91221 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 591.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) 2710 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1106 ANIMAL NUMBER: C91222 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 242.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:43 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 7.3014 ------------------------------------------- ------------------------------- 3.0159 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2711 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1107 ANIMAL NUMBER: C91222 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 242.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:43 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2712 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1108 ANIMAL NUMBER: C91223 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 213.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:06 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0320 .0307 1.7695 .7838 .7669 6.6720 1.0099 .0434 .0275 .3311 .0061 .0098 .4363 .0150 % .0144 % .8273 % .3664 % .3585 % 3.1192 % .4721 % .0203 % .0129 % .1548 % .0029 % .0046 % .2040 % .0181 .0173 1.0000 .4430 .4334 3.7706 .5707 .0245 .0155 .1871 .0034 .0055 .2466 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2713 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1109 ANIMAL NUMBER: C91223 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 213.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:06 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2714 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1110 ANIMAL NUMBER: C91224 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/02/99 STUDY DAY OF DEATH: 676 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 12/02/99 14:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >DISCHARGE; GENITAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 7 MM IN DIAMETER; SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTION OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, RED, DARK RED, GREY, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -TELANGIECTASIS,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL 2715 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1111 ANIMAL NUMBER: C91224 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/02/99 STUDY DAY OF DEATH: 676 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 12/02/99 14:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); INGUINAL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2716 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1112 ANIMAL NUMBER: C91224 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/02/99 STUDY DAY OF DEATH: 676 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 12/02/99 14:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2717 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1113 ANIMAL NUMBER: C91225 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 446.7 GRAMS DATE AND TIME OF NECROPSY: 09/02/99 8:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; SWOLLEN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PANCREAS (PA) : -LARGE VESSELS; UP TO 3 MM IN DIAMETER; COLLECTED INTACT LN, MESENTERIC (MS) : -LARGE; UP TO 5 MM IN DIAMETER OVARY (OV) : -MISSING/NOT IDENTIFIED; LEFT: NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE PANCREAS (PA) : -I-ENDOMETRIAL STROMAL SARCOMA,NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : >TISSUE MISSING OVARY (OV) : -ONE EXAMINED,-PRESENT 2718 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1114 ANIMAL NUMBER: C91225 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 446.7 GRAMS DATE AND TIME OF NECROPSY: 09/02/99 8:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY THYROID (TY) : -DIFFUSELY RED; LEFT: DARK RED PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, DARK RED AND FIRM STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK RED LINEAR AREA, 3 X 1 MM CERVIX (CV) : -MASS(ES); INVOLVING ENTIRE CERVIX: MULTIPLE MASSES, UP TO 64 X 34 X 33 MM, ALL ARE RED, DARK RED, TAN, GELATINOUS, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) POSSIBLY CONSISTENT WITH SWOLLEN ABDOMEN NOTED IN CLINICAL OBSERVATIONS LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE CERVIX (CV) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -NECROSIS,-MODERATELY SEVERE, MULTI FOCAL -M-SARCOMA, ENDOMETRIAL STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -ENDOMETRIAL STROMAL SARCOMA,-PRESENT CAVITY, ABDOM (PC) : -I-ENDOMETRIAL STROMAL SARCOMA,NEOPLASM PRESENT LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2719 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1115 ANIMAL NUMBER: C91225 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 446.7 GRAMS DATE AND TIME OF NECROPSY: 09/02/99 8:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BROKEN AT BASE: BILATERAL, UPPER INCISORS 2720 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1116 ANIMAL NUMBER: C91225 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/02/99 STUDY DAY OF DEATH: 585 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 446.7 GRAMS DATE AND TIME OF NECROPSY: 09/02/99 8:07 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2721 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1117 ANIMAL NUMBER: C91226 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 377.8 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:15 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 59; 40 X 45MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 64; 40 X 60MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 74; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 78; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 11 X 8 X 7 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL OVARY (OV) : -CYST,-PRESENT -MINERALIZATION,-MINIMAL, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2722 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1118 ANIMAL NUMBER: C91226 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 377.8 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:15 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 30 X 25 X 15 MM, RED, DARK RED, FIRM AND LOBULATED; ON CUT SURFACE EXUDES A TAN SEMISOLID; COLLECTED; REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 45 X 40 X 20 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 60 X 38 X 30 MM, RED, DARK RED, TAN, BROWN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 2723 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1119 ANIMAL NUMBER: C91226 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 377.8 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:15 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #4; RIGHT INGUINAL REGION: SINGLE MASS, 36 X 24 X 24 MM, RED, DARK RED, TAN AND FIRM; ON SECTION, FILLED WITH A DARK BROWN FLUID AND A TAN SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : >UNREMARKABLE 2724 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1120 ANIMAL NUMBER: C91226 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 377.8 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:15 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2725 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1121 ANIMAL NUMBER: C91227 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:34 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 84; 100 X 70MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 86; 85 X 65MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE RIGHT GLAND: SINGLE MASS, 10 MM IN DIAMETER, DARK RED, BROWN AND FIRM; COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED PITUITARY (PI) : -RAISED AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -THROMBUS,-MODERATE, FOCAL LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,SLIGHT, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 2726 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1122 ANIMAL NUMBER: C91227 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:34 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERINEAL AND PERIANAL REGIONS: SINGLE MASS, 80 X 55 X 25 MM (WEIGHS 58.2 GRAMS), TAN, GREY, RED, FIRM AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION, EXTENDING INTO RIGHT PERIANAL REGION: SINGLE MASS, 100 X 60 X 40 MM (WEIGHS 125.2 GRAMS), TAN, RED, FIRM AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2727 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1123 ANIMAL NUMBER: C91227 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:34 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), THYROID (TY), URINARY BLADDER (UB) 2728 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1124 ANIMAL NUMBER: C91228 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 313.5 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 82; 25 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 9 X 7 MM, RED, DARK RED, LOBULATED AND FIRM STOMACH, NONGL (SU) : -DARK FOCUS(I)/a REA(S); MUCOSA: MULTIPLE DARK BROWN PINPOINT FOCI HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, DIFFUSE -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2729 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1125 ANIMAL NUMBER: C91228 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 313.5 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 30 X 27 X 15 MM, TAN, GREY, LOBULATED AND FIRM: SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOID SEMIFLUID MATERIAL LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 21) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST, BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, DARK RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY, RED MATERIAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT VAGINA (VA) : ^UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : >UNREMARKABLE 2730 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1126 ANIMAL NUMBER: C91228 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 313.5 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JODI THIEREN RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2731 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1127 ANIMAL NUMBER: C91229 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 251.4 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 82; 50 X 45MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AREA, 15 X 10 MM; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL OVARY (OV) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT 2732 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1128 ANIMAL NUMBER: C91229 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 251.4 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY THYMUS (TH) : -MISSING/NOT IDENTIFIED; NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, DARK RED AND SOFT SKIN (SK) : >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT INGUINAL REGION MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 45 X 40 X 35 MM, DARK RED, BROWN, TAN, LOBULATED AND SOFT; ON SECTION EXUDES A BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) >NOTE:>ROUTINE SECTION TAKEN FROM RIGHT INGUINAL REGION DUE TO MASS ON LEFT INGUINAL REGION VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOID SEMIFLUID MATERIAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT VAGINA (VA) : ^UNREMARKABLE 2733 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1129 ANIMAL NUMBER: C91229 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 251.4 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:40 PROSECTOR: JARED FISHEL RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN), LN, INGUINAL (SG), (IN) 2734 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1130 ANIMAL NUMBER: C91230 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 6.6675 ------------------------------------------- ------------------------------- 3.1765 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2735 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1131 ANIMAL NUMBER: C91230 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.9 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2736 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1132 ANIMAL NUMBER: C91231 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 467.1 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:06 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 8 X 8 MM, RED, GRAY, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -VACUOLATION,-MODERATE, FOCAL OVARY (OV) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 2737 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1133 ANIMAL NUMBER: C91231 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 467.1 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:06 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA, TISSUE SURROUNDING PYLORIC VALVE: MULTIPLE DARK BROWN FOCI, UP TO 1 MM IN DIAMETER -THICKENED WALL; MUCOSA STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN, SLIGHTLY ERODED AREAS, UP TO 2 X 1 MM -THICKENED WALL; MUCOSA SKIN (SK) : -CRUSTED AREA(S); ATTACHED TO RIGHT LOWER LIP: SINGLE DRY AND TAN CRUSTED AREA, 5 X 3 MM (CAP 21) CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO OMENTUM, CRANIAL TO LEFT OVARY: SINGLE MASS, 25 X 10 X 10 MM, DARK BROWN, GRAY, TAN, GREEN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: BROWN, MOIST MATERIAL STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL SKIN (SK) : -ACUTE INFLAMMATORY EXUDATE,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT CAVITY, ABDOM (PC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -NECROSIS, FAT,-PRESENT 2738 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1134 ANIMAL NUMBER: C91231 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 467.1 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:06 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY, RED MATERIAL -ANIMAL OBESE 2739 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1135 ANIMAL NUMBER: C91231 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 639 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 467.1 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:06 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2740 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1136 ANIMAL NUMBER: C91232 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY CERVIX (CV) : -MASS(ES); WITHIN LUMEN: SINGLE MASS 9 X 8 X 6 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT CERVIX (CV) : -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2741 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1137 ANIMAL NUMBER: C91232 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 9:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) 2742 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1138 ANIMAL NUMBER: C91233 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 247.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:25 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0433 .0344 1.9921 1.0609 1.0182 7.5938 1.1176 .0518 .0586 .4480 .0064 .0129 .4988 .0175 % .0139 % .8062 % .4293 % .4121 % 3.0732 % .4523 % .0210 % .0237 % .1813 % .0026 % .0052 % .2019 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0217 .0173 1.0000 .5326 .5111 3.8120 .5610 .0260 .0294 .2249 .0032 .0065 .2504 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2743 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1139 ANIMAL NUMBER: C91233 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 247.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:25 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2744 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1140 ANIMAL NUMBER: C91234 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/29/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/29/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND TAN PITUITARY (PI) : -MASS(ES); 7 X 7 X 6 MM, DARK RED, RED, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 X 2 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, FOCAL -PYELONEPHRITIS,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2745 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1141 ANIMAL NUMBER: C91234 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/29/99 STUDY DAY OF DEATH: 459 STUDY WEEK OF DEATH: 66 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/29/99 11:13 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2746 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1142 ANIMAL NUMBER: C91235 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); JUNCTION OF RIGHT AND LEFT MEDIAN LOBES: SINGLE TAN AREA, 4 X 3 MM; EXTRA SECTION COLLECTED KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN PELVIS: MULTIPLE WHITE PINPOINT GRANULES ADRENAL, CORTEX (AC) : -CYST(S); RIGHT, WITHIN CORTEX: MULTIPLE RED FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK BROWN AREA, 2 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-SEVERE, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2747 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1143 ANIMAL NUMBER: C91235 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CERVIX (CV) : -LARGE; DIFFUSE: 8 MM IN DIAMETER LN, MEDIASTINAL (ML) : -LARGE; UP TO 4 X 3 X 1 MM -DIFFUSELY RED; DARK RED (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CERVIX (CV) : -HYPERTROPHY, SMOOTH MUSCLE,-MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : >TISSUE MISSING >NOTE:>MEDIASTINAL LYMPH NODE DESCRIBED GROSSLY WAS ADIPOSE TISSUE ADIPOSE TISSUE (AT) : -FIBROSIS,-MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2748 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1144 ANIMAL NUMBER: C91235 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:34 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 2749 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1145 ANIMAL NUMBER: C91236 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 442.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:43 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 93; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DARK FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK BROWN AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 5 X 4 MM, RED, DARK RED, DARK BROWN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -TELANGIECTASIS,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE, MULTI-FOCAL THYROID (TY) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2750 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1146 ANIMAL NUMBER: C91236 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 442.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:43 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 30 X 20 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2751 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1147 ANIMAL NUMBER: C91236 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 442.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:43 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) (MS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB), LN, AXILLARY (AX) 2752 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1148 ANIMAL NUMBER: C91237 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 194.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:00 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 6.4697 ------------------------------------------- ------------------------------- 3.3246 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2753 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1149 ANIMAL NUMBER: C91237 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 194.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:00 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2754 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1150 ANIMAL NUMBER: C91238 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 280.5 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:53 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, BROWN TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 20 X 15 X 10 MM, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2755 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1151 ANIMAL NUMBER: C91238 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 280.5 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:53 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; CHEST AND FORELIMBS: RED AND DRY -GI TRACT-DARK FLUID CONTENT; ENTIRE GI TRACT: DARK BROWN, TENACIOUS SEMIFLUID 2756 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1152 ANIMAL NUMBER: C91238 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 280.5 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 13:53 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2757 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1153 ANIMAL NUMBER: C91239 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 399.2 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 35; 45 X 45MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 47; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 4 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2758 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1154 ANIMAL NUMBER: C91239 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 399.2 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 60 X 55 X 25 MM, RED, DARK RED, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A SINGLE RED AND DRY ULCERATED AREA, 20 X 17 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION AND EXTRA SECTION COLLECTED (CAP 20); POSSIBLY CONSISTENT WITH MASS (LEFT AXILLARY) NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 9 X 6 X 5 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT 2759 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1155 ANIMAL NUMBER: C91239 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 399.2 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 13:43 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU),HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2760 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1156 ANIMAL NUMBER: C91240 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 550 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 382.6 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 15:27 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 74; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 25 X 18 X 15 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SEVERE, FOCAL 2761 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1157 ANIMAL NUMBER: C91240 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 550 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 382.6 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 15:27 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -CYST(S); LEFT, WITHIN HORN: SINGLE CLEAR FLUID FILLED CYST, 22 X 10 X 10 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2762 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1158 ANIMAL NUMBER: C91240 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 550 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 382.6 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 15:27 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2763 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1159 ANIMAL NUMBER: C91241 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 9:12 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0404 .0423 2.0015 1.0454 1.1192 9.1320 1.3728 .0433 .0524 .5501 .0081 .0083 .6220 .0140 % .0147 % .6957 % .3634 % .3890 % 3.1741 % .4772 % .0151 % .0182 % .1912 % .0028 % .0029 % .2162 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0202 .0211 1.0000 .5223 .5592 4.5626 .6859 .0216 .0262 .2748 .0040 .0041 .3108 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSIED OUT OF ORDER DUE TO MORIBUND COLLECTION FOLLOWING SAMPLE COLLECTION LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2764 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1160 ANIMAL NUMBER: C91241 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.7 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 9:12 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2765 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1161 ANIMAL NUMBER: C91242 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 437.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:13 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 6 MM IN DIAMETER, RIGHT IS 9 MM IN DIAMETER MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -MASS(ES); MID PORTION OF RIGHT HORN, WITHIN LUMEN: SINGLE MASS, 7 X 5 X 5 MM, RED, DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, FOCAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : >UNREMARKABLE UTERUS (UT) : -DILATATION,-MODERATE, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT 2766 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1162 ANIMAL NUMBER: C91242 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 437.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:13 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) 2767 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1163 ANIMAL NUMBER: C91243 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 68; 75 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 89; 30 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 93; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); CAUDATE LOBE: SINGLE TAN AREA, 6 MM IN DIAMETER; COLLECTED -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR FLUID FILLED CYSTS ON EACH, UP TO 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, FOCAL THYROID (TY) : -HEMORRHAGE,-SLIGHT, FOCAL 2768 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1164 ANIMAL NUMBER: C91243 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 4 X 4 MM PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, TAN, SOFT AND LOBULATED SKIN (SK) : -MASS(ES); DORSAL THORACIC REGION: SINGLE MASS, 15 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; OVERLYING SURFACE HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 5 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERIANAL REGION: SINGLE MASS, 60 X 40 X 25 MM, RED, DARK RED, TAN AND LOBULATED; ON SECTION EXUDES A DARK GREEN SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 45 X 35 X 25 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -ULCERATION,-SLIGHT, FOCAL -M-SEBACEOUS GLAND CARCINOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 2769 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1165 ANIMAL NUMBER: C91243 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT AXILLARY REGION: SINGLE MASS, 90 X 50 X 30 MM, TAN, FIRM AND LOBULATED; SOME SECTIONS EXUDE A LARGE AMOUNT OF A TAN, CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 10 X 6 X 3 MM (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -DILATATION, SINUSOIDAL,-MINIMAL, MULTI FOCAL 2770 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1166 ANIMAL NUMBER: C91243 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 564.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:33 PROSECTOR: JARED FISHEL RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB) 2771 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1167 ANIMAL NUMBER: C91244 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 316.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 13:57 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; VOCALIZATION; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 6 MM, TAN, RED AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CONTAINS A LIGHT GREEN SEMIFLUID HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE 2772 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1168 ANIMAL NUMBER: C91244 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 316.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 13:57 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY -MALOCCLUSION; BILATERAL UPPER INCISORS CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2773 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1169 ANIMAL NUMBER: C91244 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/07/99 STUDY DAY OF DEATH: 467 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 316.8 GRAMS DATE AND TIME OF NECROPSY: 05/07/99 13:57 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2774 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1170 ANIMAL NUMBER: C91245 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 413.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 14:19 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 7 X 5 MM (LEFT IS 6 X 5 X 3 MM) PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SEVERE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 2775 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1171 ANIMAL NUMBER: C91245 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 413.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 14:19 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2776 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1172 ANIMAL NUMBER: C91245 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/24/99 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 413.7 GRAMS DATE AND TIME OF NECROPSY: 11/24/99 14:19 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2777 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1173 ANIMAL NUMBER: C91246 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:56 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); AXILLARY REGION-LEFT; WEEK 68; 105 X 75MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -MOTTLED; BILATERAL: TAN AND BROWN STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE RED AREAS, UP TO 2 X 3 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 25 X 55 X 100 MM (88.4 GRAMS) FIRM, LOBULATED, RED, DARK RED, TAN AND VASCULAR; ON SECTION CONTAINS A LARGE AMOUNT OF DARK BROWN FLUID (COLLECTED); REGIONAL LN (SEE AXILLARY) HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -NECROSIS,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, FOCAL STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -NECROSIS,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2778 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1174 ANIMAL NUMBER: C91246 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:56 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, AXILLARY (AX) : -MOTTLED; RIGHT: RED AND TAN (CAP 21) -DIFFUSELY RED; LEFT: DARK RED (CAP 21) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, FOCAL CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2779 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1175 ANIMAL NUMBER: C91246 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:56 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM),MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2780 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1176 ANIMAL NUMBER: C91247 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:15 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 64; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 70; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; ORAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -UNCOLLAPSED; ALL LOBES HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -EDEMA, ALVEOLAR,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 2781 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1177 ANIMAL NUMBER: C91247 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:15 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 14 X 9 X 8 MM, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 20 X 17 X 8 MM, TAN, RED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); LEFT PERIANAL REGION: TWO TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 8 X 6 X 3 MM (CAP 20) -MASS #2; RIGHT LATERAL THORACIC REGION: SINGLE MASS, 33 X 27 X 13 MM, TAN, GRAY, RED, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; RED AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT VAGINA (VA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2782 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1178 ANIMAL NUMBER: C91247 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:15 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2783 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1179 ANIMAL NUMBER: C91248 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 283.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:33 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0313 .0306 2.0203 .9436 .9676 7.5181 1.3491 .0662 .0617 .5114 .0118 .0113 .5209 .0110 % .0108 % .7126 % .3328 % .3413 % 2.6519 % .4759 % .0234 % .0218 % .1804 % .0042 % .0040 % .1837 % .0155 .0151 1.0000 .4671 .4789 3.7213 .6678 .0328 .0305 .2531 .0058 .0056 .2578 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2784 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1180 ANIMAL NUMBER: C91248 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 283.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:33 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2785 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1181 ANIMAL NUMBER: C91249 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 405.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 9 X 7 X 7 MM, DARK RED, TAN AND SOFT JEJUNUM (JE) : -DIVERTICULUM(A); MID JEJUNUM: SINGLE DARK RED DIVERTICULUM, 12 X 12 X 10 MM; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -CYST(S); LEFT ABDOMINAL REGION: SINGLE TAN CREAMY SEMIFLUID FILLED CYST, 10 X 6 X 5 MM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL 2786 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1182 ANIMAL NUMBER: C91249 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 405.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CAVITY, ABDOM (PC) : -MASS(ES); ANTERIOR TO RIGHT OVARY: SINGLE MASS, 22 X 20 X 12 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY DARK BROWN MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT CAVITY, ABDOM (PC) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL -NECROSIS, FAT,-PRESENT 2787 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1183 ANIMAL NUMBER: C91249 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 405.4 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:48 PROSECTOR: DAVID SCHUETTE RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUm /s KULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 2788 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1184 ANIMAL NUMBER: C91250 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 487.9 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 15:14 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 38; 100 X 100MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 120 X 100 X 40 MM, RED, DARK RED, TAN, VASCULAR, AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20); OVERLYING SKIN OF MASS HAS A SINGLE DARK BROWN CRUSTED AREA, 25 X 10 MM; COLLECTED INTACT WITH MASS PORTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL OVARY (OV) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-SEVERE, FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2789 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1185 ANIMAL NUMBER: C91250 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 487.9 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 15:14 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS UTERUS (UT) : -DILATATION,-SLIGHT, DIFFUSE CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT 2790 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1186 ANIMAL NUMBER: C91250 SEX: FEMALE DOSE GROUP: 2 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 487.9 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 15:14 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2791 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1187 ANIMAL NUMBER: C91251 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:53 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 7.0583 ------------------------------------------- ------------------------------- 3.3756 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2792 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1188 ANIMAL NUMBER: C91251 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 209.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:53 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2793 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1189 ANIMAL NUMBER: C91252 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 600.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:06 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 MM IN DIAMETER (RIGHT IS 4 MM IN DIAMETER) PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ANTERIOR ASPECT: SINGLE DARK RED AREA, 5 X 3 MM -LARGE; 7 X 7 X 3 MM; APPEARS SWOLLEN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2794 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1190 ANIMAL NUMBER: C91252 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 600.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:06 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) 2795 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1191 ANIMAL NUMBER: C91253 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 542.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:35 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 105; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 50 X 34 X 20 MM, TAN, LOBULATED AND SOFT; SOME PORTIONS EXUDE A TAN CREAMY SEMISOLID MATERIAL; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2796 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1192 ANIMAL NUMBER: C91253 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 542.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:35 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) 2797 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1193 ANIMAL NUMBER: C91254 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 396.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:00 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 10 MM IN DIAMETER UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL OVARY (OV) : -CYST,-PRESENT UTERUS (UT) : -DILATATION,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2798 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1194 ANIMAL NUMBER: C91254 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 396.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 13:00 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF NEAR BASE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON(TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), THYROID (TY), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) 2799 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1195 ANIMAL NUMBER: C91255 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/22/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 354.6 GRAMS DATE AND TIME OF NECROPSY: 06/22/99 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 62; 20 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 64; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL PITUITARY (PI) : -MASS(ES); 11 X 7 X 6 MM, DARK RED, VASCULAR AND SOFT JEJUNUM (JE) : >NOTE:>TRIMMING COMMENT: JEJUNUM MISSING FROM WET TISSUE HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -EDEMA,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >TISSUE MISSING 2800 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1196 ANIMAL NUMBER: C91255 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/22/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 354.6 GRAMS DATE AND TIME OF NECROPSY: 06/22/99 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 36 X 28 X 23 MM, TAN, RED, LOBULATED, CYSTIC AND FIRM; ON SECTION, EXUDES A CLEAR YELLOW FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 32 X 19 X 9 MM, TAN, RED, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN HAS A TAN MOIST ABRADED AREA, 8 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (SEE AXILLARY) LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 6 X 4 X 1 MM (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN RECTUM (RE) : -PARASITISM,-PRESENT SKIN (SK) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -ULCERATION,-SLIGHT, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,- 2 NEOPLASMS PRESENT UTERUS (UT) : -DILATATION,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL 2801 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1197 ANIMAL NUMBER: C91255 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/22/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 354.6 GRAMS DATE AND TIME OF NECROPSY: 06/22/99 7:55 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2802 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1198 ANIMAL NUMBER: C91256 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 379.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 90; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 MM IN DIAMETER (RIGHT IS 5 MM IN DIAMETER) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED, TAN, GRAY, AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); PERINEAL REGION: SINGLE MASS, 87 X 50 X 28 MM, RED, DARK RED, DARK BROWN, TAN, GRAY, LOBULATED, VASCULAR AND FIRM; ON CUT SECTION EXUDES A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2803 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1199 ANIMAL NUMBER: C91256 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 379.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: 13 X 10 X 10 MM (RIGHT IS 10 X 8 X 6 MM); COLLECTED LN, POPLITEAL (PP) : -LARGE; LEFT: UP TO 8 X 6 X 3 MM (RIGHT IS UP TO 5 X 3 X 2 MM); APPEARS CYSTIC (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT JOINT, OTHER (JT) : -EXOSTOSES,-MODERATE, MULTI-FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL LN, POPLITEAL (PP) : -DILATATION, SINUSOIDAL,-MODERATE MULTI-FOCAL 2804 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1200 ANIMAL NUMBER: C91256 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 379.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 12:53 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 2805 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1201 ANIMAL NUMBER: C91257 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 219.4 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:43 PROSECTOR: DAVID SCHUETTE RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LIGHT FOCUS(I)/AREA(S); BILATERAL, DIFFUSE: MULTIPLE PINPOINT TAN AREAS PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 4 MM TAN, DARK RED, AND SOFT; COLLECTED INTACT WITH BRAIN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, CORTICAL CELL,-MINIMAL, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2806 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1202 ANIMAL NUMBER: C91257 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 219.4 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:43 PROSECTOR: DAVID SCHUETTE RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: MOIST BROWN MATERIAL -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION 2807 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1203 ANIMAL NUMBER: C91257 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 219.4 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:43 PROSECTOR: DAVID SCHUETTE RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2808 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1204 ANIMAL NUMBER: C91258 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 283.6 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 79; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 71; 45 X 50MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, DARK RED, GRAY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL 2809 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1205 ANIMAL NUMBER: C91258 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 283.6 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 30 X 30 X 25 MM, RED, DARK RED, BROWN, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 45 X 45 X 35 MM, TAN, BROWN, VASCULAR AND SOFT; ON SECTION EXUDE A TAN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED -ANIMAL THIN MAMMARY, FEMALE (MF) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2810 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1206 ANIMAL NUMBER: C91258 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 283.6 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2811 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1207 ANIMAL NUMBER: C91259 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/08/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/08/99 13:02 PROSECTOR: JOHN S. HALFORD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE GRAY AND TAN AREA, 7 X 5 MM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); 14 X 10 X 9 MM, RED, DARK RED, GRAY, TAN, AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN, MUCOID SEMIFLUID GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FIBROSIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2812 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1208 ANIMAL NUMBER: C91259 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/08/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/08/99 13:02 PROSECTOR: JOHN S. HALFORD RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -AUTOLYSIS-SEVERE; INVOLVING JEJUNUM AND ILEUM THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2813 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1209 ANIMAL NUMBER: C91260 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 312.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 8:47 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (FOR COMPARISON, LEFT IS 5 MM IN DIAMETER) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL LIVER (LI) : -HEMORRHAGE,-MINIMAL, FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL OVARY (OV) : >UNREMARKABLE 2814 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1210 ANIMAL NUMBER: C91260 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 312.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 8:47 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 9 X 8 MM DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BROWN AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2815 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1211 ANIMAL NUMBER: C91260 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 312.7 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 8:47 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2816 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1212 ANIMAL NUMBER: C91261 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 10:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADCRANIAL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 87; 60 X 70MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; LEFT BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 9 X 7 MM, DARK RED, GREY, LOBULATED AND SOFT HISTOPATHOLOGY EYE (EY) : >UNREMARKABLE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2817 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1213 ANIMAL NUMBER: C91261 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 10:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 65 X 45 X 33 MM, RED, DARK RED, TAN, GREY, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; DRY, RED MATERIAL -ANIMAL THIN ENTIRE HEAD: JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2818 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1214 ANIMAL NUMBER: C91261 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 10:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) (SM), 2819 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1215 ANIMAL NUMBER: C91262 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 298.9 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:57 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC; WEEK 81; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; DEHYDRATED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT ADRENAL, CORTEX (AC) : -DIFFUSELY LIGHT; BILATERAL: TAN LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED PITUITARY (PI) : -MASS(ES); 5 MM IN DIAMETER, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT >NOTE:>RIGHT KIDNEY ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CERVIX (CV) : -M-SARCOMA, ENDOMETRIAL STROMAL,NEOPLASM PRESENT 2820 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1216 ANIMAL NUMBER: C91262 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 298.9 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:57 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CERVIX (CV) : -MASS(ES); INVOLVING ENTIRE CERVIX: SINGLE MASS, 50 X 45 X 30 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) VAGINA (VA) : -MASS(ES); MUCOSA: SINGLE RED, TAN, GELATINOUS, AND PEDUNCULATED MASS, 20 X 12 X 6 MM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: LEFT IS 18 X 14 X 10 MM, RIGHT IS 50 X 40 X 40 MM; BOTH APPEAR CYSTIC (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: YELLOW AND MOIST -ANIMAL THIN VAGINA (VA) : -I-ENDOMETRIAL STROMAL SARCOMA,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -ENDOMETRIAL STROMAL SARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -N-ENDOMETRIAL STROMAL SARCOMA,NEOPLASM PRESENT 2821 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1217 ANIMAL NUMBER: C91262 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 618 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 298.9 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 14:57 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 2822 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1218 ANIMAL NUMBER: C91263 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.5 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 15:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 22; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 9 X 6 X 5 MM, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT LUNG (LU) : -HEMORRHAGE,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2823 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1219 ANIMAL NUMBER: C91263 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.5 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 15:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 45 X 35 X 15 MM, RED, DARK RED, GRAY, TAN AND SOFT; CUT SURFACE EXUDES A DARK GREEN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW, BROWN AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2824 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1220 ANIMAL NUMBER: C91263 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.5 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 15:10 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2825 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1221 ANIMAL NUMBER: C91264 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:38 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 93; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 97; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -LARGE; UP TO 8 MM IN DIAMETER THYROID (TY) : -LARGE; RIGHT: 8 X 4 X 2 MM (LEFT IS 5 X 3 X 1 MM) PITUITARY (PI) : -MASS(ES); 11 X 8 X 8 MM, DARK RED, TAN AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 35 X 30 X 18 MM, TAN, BROWN, LOBULATED, VASCULAR AND SOFT; ON SECTION EXUDES A BROWN FLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LN, MESENTERIC (MS) : >UNREMARKABLE THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,- 2 NEOPLASMS PRESENT 2826 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1222 ANIMAL NUMBER: C91264 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:38 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 27 X 27 X 15 MM, TAN, RED, DARK BROWN, LOBULATED AND FIRM; ON CUT SURFACE EXUDES A DARK RED FLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) UTERUS (UT) : -CYST(S); LEFT, WITHIN WALLS: TWO CLEAR FLUID FILLED CYSTS, BOTH ARE 5 X 4 X 4 MM LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -DILATATION,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2827 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1223 ANIMAL NUMBER: C91264 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 398.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:38 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB), LN, AXILLARY (AX) 2828 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1224 ANIMAL NUMBER: C91265 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 7:24 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 10 X 6 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL 2829 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1225 ANIMAL NUMBER: C91265 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 7:24 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MUCOID SEMIFLUID MATERIAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: YELLOW MOIST MATERIAL; POSSIBLY CONSISTENT WITH YELLOW HAIRCOAT AS NOTED IN CLINICAL OBSERVATIONS -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2830 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1226 ANIMAL NUMBER: C91265 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 7:24 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 2831 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1227 ANIMAL NUMBER: C91266 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 309.0 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 11:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 89; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 97; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 10 X 4 MM, DARK RED, LOBULATED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, DIFFUSE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY, CORTICAL CELL,-MODERATE, MULTI-FOCAL -VACUOLATION,-MINIMAL, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL 2832 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1228 ANIMAL NUMBER: C91266 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 309.0 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 11:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE MOIST TAN ULCERATED AREA, 7 X 4 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 40 X 30 X 10 MM, TAN, GREEN, RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 25 X 15 X 15 MM, TAN, RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 21) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: SINGLE DRY BROWN CRUSTED AREA, 5 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,- 2 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -FIBROSIS,-MODERATELY SEVERE, FOCAL -INFLAMMATION, CHRONIC ACTIVE,-SLIGHT, MULTI-FOCAL 2833 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1229 ANIMAL NUMBER: C91266 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 723 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 309.0 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 11:30 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) (SM), 2834 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1230 ANIMAL NUMBER: C91267 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 404.8 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:08 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 38; 25 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 46; 70 X 70MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK BROWN AREA, 3 X 2 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT CERVICAL REGION: SINGLE MASS, 75 X 70 X 30 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (COLLECTED); REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) HISTOPATHOLOGY SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SKIN (SK) : -ULCERATION,-SEVERE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 2835 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1231 ANIMAL NUMBER: C91267 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 404.8 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:08 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT ABDOMINAL REGION: SINGLE MASS, 45 X 30 X 15 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (COLLECTED); REGIONAL LN (INGUINAL) NORMAL (CAP 21); SKIN OVERLYING MASS HAS A SINGLE TAN, ABRADED AREA, 45 X 20 MM; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION 2836 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1232 ANIMAL NUMBER: C91267 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 404.8 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:08 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) 2837 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1233 ANIMAL NUMBER: C91268 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 618.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK 80; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >EYES; DISCOLORED/DARK RED; EYE-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE DARK RED AREA, 7 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT LATERAL ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL 2838 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1234 ANIMAL NUMBER: C91268 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 618.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -MASS(ES); LEFT LATERAL CERVICAL REGION, EXTENDING ALONG LEFT MANDIBLE: SINGLE MASS, 40 X 35 X 30 MM, RED, DARK RED, TAN AND SOFT; ON SECTION EXUDES A TAN SEMIFLUID; COLLECTED; REGIONAL LN (SEE MANDIBULAR) LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED CRUSTED AREA, 5 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -KERATOACANTHOMA,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 2839 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1235 ANIMAL NUMBER: C91268 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 618.5 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:04 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2840 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1236 ANIMAL NUMBER: C91269 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 295.3 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -GRANULAR MATERIAL; LEFT, WITHIN PELVIS: MULTIPLE TAN PINPOINT GRANULES PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2841 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1237 ANIMAL NUMBER: C91269 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 295.3 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK BROWN AND DRY MATERIAL -DISCHARGE-NASAL; DARK BROWN AND DRY MATERIAL 2842 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1238 ANIMAL NUMBER: C91269 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 295.3 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 14:00 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2843 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1239 ANIMAL NUMBER: C91270 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 226.6 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); BILATERAL, WITHIN CORTEX: MULTIPLE BROWN AREAS ON EACH, EACH IS UP TO 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 9 X 8 X 5 MM, RED, DARK RED, TAN, GREY AND SOFT MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 2844 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1240 ANIMAL NUMBER: C91270 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 226.6 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2845 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1241 ANIMAL NUMBER: C91270 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 226.6 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2846 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1242 ANIMAL NUMBER: C91271 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 13:36 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 84; 35 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 84; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 89; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; GENITAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: RIGHT IS 7 X 5 X 4 MM, LEFT IS 6 X 5 X 3 MM PITUITARY (PI) : -MASS(ES); 11 X 10 X 7 MM, RED, DARK RED, TAN, LOBULATED AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 11 X 10 X 6 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (AXILLARY) NORMAL (CAP 22) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -B-FIBROADENOMA,- 4 NEOPLASMS PRESENT 2847 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1243 ANIMAL NUMBER: C91271 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 13:36 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 45 X 38 X 26 MM, RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 40 X 30 X 16 MM, RED, TAN, LOBULATED, SOME AREAS ARE SOFT AND SOME AREAS ARE FIRM; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A GREEN, CREAMY SEMIFLUID (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT LATERAL ABDOMINAL REGION: SINGLE MASS, 25 X 18 X 5 MM, RED, TAN, DARK GREEN, LOBULATED AND SOFT; ON SECTION EXUDES A GREEN, CREAMY SEMIFLUID (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -MASS(ES); WITHIN LUMEN: SINGLE MASS, 12 X 10 X 6 MM, RED, TAN AND FIRM; ON SECTION EXUDES A CLEAR FLUID; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 26) VAGINA (VA) : -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SQUAMOUS CELL CARCINOMA,-PRESENT 2848 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1244 ANIMAL NUMBER: C91271 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 13:36 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST RED AND YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -ANIMAL THIN 2849 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1245 ANIMAL NUMBER: C91271 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/09/99 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 314.5 GRAMS DATE AND TIME OF NECROPSY: 11/09/99 13:36 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX) 2850 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1246 ANIMAL NUMBER: C91272 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 206.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:06 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 7.4750 ------------------------------------------- ------------------------------- 3.6269 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2851 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1247 ANIMAL NUMBER: C91272 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 206.1 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:06 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2852 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1248 ANIMAL NUMBER: C91273 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 354.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 13:13 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 76; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 7 X 9 MM, SOFT, RED, DARK RED AND TAN MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 7 X 10 X 14 MM, FIRM, RED, DARK RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 2853 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1249 ANIMAL NUMBER: C91273 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 354.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 13:13 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: FIRM AND TAN; COLLECTED WITH ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE 2854 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1250 ANIMAL NUMBER: C91273 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 354.7 GRAMS DATE AND TIME OF NECROPSY: 08/09/99 13:13 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2855 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1251 ANIMAL NUMBER: C91274 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/30/99 13:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 11 X 8 MM, DARK RED, GREY, TAN, LOBULATED, CYSTIC AND SOFT; ON SECTION EXUDES A CLEAR FLUID HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL 2856 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1252 ANIMAL NUMBER: C91274 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/30/99 13:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -LUMEN FILLED WITH FLUID; RIGHT: DARK RED FLUID LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED AND TAN; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST, YELLOW MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL UTERUS (UT) : -DILATATION,-MODERATE, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE 2857 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1253 ANIMAL NUMBER: C91274 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/30/99 STUDY DAY OF DEATH: 490 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/30/99 13:58 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2858 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1254 ANIMAL NUMBER: C91275 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 296.5 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 9:12 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 58; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, DARK RED, GRAY, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL MUSCLE, SKELETAL (SM) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SEVERE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2859 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1255 ANIMAL NUMBER: C91275 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 296.5 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 9:12 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); LEFT SHOULDER REGION: SINGLE CYST, 20 MM IN DIAMETER, FILLED WITH A DARK BROWN, TENACIOUS SEMIFLUID AND BROWN, FRIABLE SEMISOLID MATERIAL (CAP 20); POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS -THICKENED; ALL REGIONS, DIFFUSE: TAN, FIRM, AND CYSTIC (CAP 21) TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE BROWN, DRY, CRUSTED AREAS, UP TO 5 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -ANIMAL THIN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : -EROSION,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 2860 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1256 ANIMAL NUMBER: C91275 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 296.5 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 9:12 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2861 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1257 ANIMAL NUMBER: C91276 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 62; 30 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 72; 40 X 45MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 82; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL-CERVICAL-LEFT; WEEK 82; 70 X 60MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 88; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 93; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, DARK RED, TAN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -ULCERATION,-SLIGHT, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL 2862 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1258 ANIMAL NUMBER: C91276 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY >HOUSING,FOOD,WATER & MAINT.; TEETH CUT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; LATERALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 36 X 24 X 16 MM, TAN, RED, BLACK AND SOFT; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT AXILLARY, CRANIAL TO MASS #1: SINGLE MASS, 75 X 60 X 25 MM, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 80 X 27 X 19 MM, TAN, BROWN, BLACK AND SOFT; ON SECTION, EXUDES A DARK BROWN SEMIFLUID (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; RIGHT AXILLARY REGION: SINGLE MASS, 30 X 20 X 17 MM, TAN, BROWN, LOBULATED AND SOFT; ON SECTION, EXUDES A TAN CREAMY SEMISOLID (CAP 25); REGIONAL LN (SEE AXILLARY) MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT -M-CARCINOMA,- 2 NEOPLASMS PRESENT 2863 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1259 ANIMAL NUMBER: C91276 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #5; RIGHT INGUINAL REGION: SINGLE MASS, 89 X 54 X 14 MM, RED, TAN, BROWN, LOBULATED, AND SOFT; ON SECTION, EXUDES A DARK BROWN SEMIFLUID (CAP 26); REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A RED, BLACK, DRY ULCERATED AREA, 20 X 15 MM; COLLECTED INTACT WITH MASS LN, MANDIBULAR (MN) : -LARGE; LEFT: 12 X 7 X 2 MM (RIGHT IS 6 X 3 X 1 MM); COLLECTED LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 22) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, DIFFUSE LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2864 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1260 ANIMAL NUMBER: C91276 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 410.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:44 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2865 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1261 ANIMAL NUMBER: C91277 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 222.9 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 72; 30 X 50MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK BROWN AREA, 5 X 2 MM -LARGE; 7 X 4 X 3 MM; APPEARS SWOLLEN STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT HEART (HT) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -ULCERATION,-MODERATE, FOCAL 2866 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1262 ANIMAL NUMBER: C91277 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 222.9 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 50 X 45 X 25 MM, RED, DARK RED, TAN, FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL -ANIMAL THIN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -NECROSIS,-SEVERE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT 2867 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1263 ANIMAL NUMBER: C91277 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 222.9 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2868 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1264 ANIMAL NUMBER: C91278 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 420.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 105; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 105; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT AND LEFT ASPECT: SINGLE DARK RED AREA ON EACH, EACH IS 2 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 25 X 18 X 15 MM, TAN, RED, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE MANDIBULAR) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 22 X 14 X 13 MM, TAN, RED, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) CERVIX (CV) : -MASS(ES); ATTACHED TO RIGHT SEROSAL SURFACE, EXTENDING TOWARDS VAGINA: SINGLE MASS, 20 X 6 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) HISTOPATHOLOGY OVARY (OV) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -M-CARCINOMA,- 2 NEOPLASMS PRESENT CERVIX (CV) : -HYPERTROPHY, SMOOTH MUSCLE,-MODERATE, DIFFUSE 2869 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1265 ANIMAL NUMBER: C91278 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 420.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 7 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2870 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1266 ANIMAL NUMBER: C91278 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 420.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:38 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), KIDNEY (KD), PANCREAS (PA), LUNG (LU), THYROID (TY), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) 2871 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1267 ANIMAL NUMBER: C91279 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 524.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 53; 70 X 70MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 80; 20 X 20MM; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 96; 35 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALONG MARGIN OF RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 5 X 5 MM (RIGHT IS 5 X 4 X 4 MM) LN, MESENTERIC (MS) : -LARGE; UP TO 18 MM IN DIAMETER -RED FOCUS(I)/AREA(S); MULTIPLE DARK RED AREAS, UP TO 10 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-MODERATELY SEVERE, FOCAL LN, MESENTERIC (MS) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT 2872 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1268 ANIMAL NUMBER: C91279 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 524.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 8 X 7 MM, RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 20 X 15 X 15 MM, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 40 X 30 X 18 MM, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 15 X 12 X 10 MM, DARK RED, TAN AND SOFT; ON SECTION EXUDES A BROWN SEMIFLUID (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 80 X 65 X 35 MM, RED, DARK RED, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -M-CARCINOMA,- 2 NEOPLASMS PRESENT 2873 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1269 ANIMAL NUMBER: C91279 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 524.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #5; LEFT PERINEAL REGION: SINGLE MASS, 25 X 18 X 14 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 24); OVERLYING SKIN HAS A SINGLE ULCERATED AREA, 2 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); MID PORTION OF LEFT HORN, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER -RED FOCUS(I)/AREA(S); PROXIMAL END OF LEFT HORN, MUCOSAL SURFACE: SINGLE DARK RED AREA, 4 X 3 MM GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; AND MOIST -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DARK GREEN UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -DILATATION, SINUSOIDAL,-MINIMAL, MULTI FOCAL -EDEMA,-SLIGHT, MULTI-FOCAL 2874 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1270 ANIMAL NUMBER: C91279 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 524.9 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:21 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), SKIN (SK), URINARY BLADDER (UB) 2875 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1271 ANIMAL NUMBER: C91280 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 389.1 GRAMS DATE AND TIME OF NECROPSY: 12/07/99 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RAISED FOCUS(I)/AREA(S); CAUDATE LOBE, NEAR HILUS: SINGLE DARK RED, TAN AND RAISED AREA, 8 X 5 X 5 MM (CAP 21) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 X 8 X 8 MM (RIGHT IS 5 X 4 X 3 MM) PITUITARY (PI) : -MASS(ES); 11 X 8 X 5 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 1 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SEVERE, FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2876 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1272 ANIMAL NUMBER: C91280 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 389.1 GRAMS DATE AND TIME OF NECROPSY: 12/07/99 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK RED MATERIAL -DISCHARGE-NASAL; DRY DARK RED MATERIAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -HEPATOCELLULAR ADENOMA,-PRESENT 2877 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1273 ANIMAL NUMBER: C91280 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/07/99 STUDY DAY OF DEATH: 681 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 389.1 GRAMS DATE AND TIME OF NECROPSY: 12/07/99 10:51 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2878 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1274 ANIMAL NUMBER: C91281 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 263.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:30 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0475 .0385 1.9995 1.1798 1.2833 9.9915 1.2442 .0784 .0779 .4918 .0074 .0094 .5260 .0180 % .0146 % .7591 % .4479 % .4872 % 3.7933 % .4724 % .0298 % .0296 % .1867 % .0028 % .0036 % .1997 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0238 .0193 1.0000 .5900 .6418 4.9970 .6223 .0392 .0390 .2460 .0037 .0047 .2631 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 20) KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 2879 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1275 ANIMAL NUMBER: C91281 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 263.4 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:30 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN -URINARY BLADDER-SMALL LUMEN; 1 MM 4X 3X THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2880 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1276 ANIMAL NUMBER: C91282 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 274.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 9:56 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 8 X 8 MM, RED AND SOFT STOMACH, GL (ST) : -MASS(ES); PYLORIC MUCOSA: TWO BROWN ERODED AREAS, EACH IS 2 MM IN DIAMETER GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; AND DRY -BLOOD FILM TAKEN DARK BROWN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -DEPLETION, LYMPHOCYTIC,-MODERATELY SEVERE, DIFFUSE PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2881 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1277 ANIMAL NUMBER: C91282 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 274.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 9:56 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY -GI TRACT-DARK DRY CONTENT; CECUM: DARK GREEN, IMPACTED, FECAL-LIKE MATERIAL 2882 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1278 ANIMAL NUMBER: C91282 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/25/99 STUDY DAY OF DEATH: 365 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 274.4 GRAMS DATE AND TIME OF NECROPSY: 01/25/99 9:56 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) (MS), 2883 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1279 ANIMAL NUMBER: C91283 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 282.2 GRAMS DATE AND TIME OF NECROPSY: 10/03/99 13:56 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 82; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 82; 35 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -DIFFUSELY DARK; LIGHT BROWN PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, DARK RED, GRAY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MINIMAL, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL LN, MESENTERIC (MS) : >UNREMARKABLE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2884 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1280 ANIMAL NUMBER: C91283 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 282.2 GRAMS DATE AND TIME OF NECROPSY: 10/03/99 13:56 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 25 X 25 X 20 MM, TAN AND FIRM; ON SECTION CONTAINS A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 23) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 35 X 35 X 15 MM, TAN, YELLOW, LOBULATED AND SOFT; SOME SECTIONS CONTAIN A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR AND RED FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE TAN AND RAISED AREA, 10 X 6 X 6 MM; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND DRY -BLOOD FILM TAKEN -DISCHARGE-NASAL; DARK RED AND DRY -ANIMAL THIN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL 2885 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1281 ANIMAL NUMBER: C91283 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 282.2 GRAMS DATE AND TIME OF NECROPSY: 10/03/99 13:56 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2886 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1282 ANIMAL NUMBER: C91284 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 9:52 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 7 MM IN DIAMETER PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM CAVITY, THORACIC (TA) : -MASS(ES); ATTACHED TO DIAPHRAGM AND THYMUS: SINGLE MASS, 25 X 20 X 20 MM, TAN, DARK RED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 21) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -LIPOSARCOMA,-PRESENT CAVITY, THORACIC (TA) : -M-LIPOSARCOMA,-NEOPLASM PRESENT 2887 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1283 ANIMAL NUMBER: C91284 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 9:52 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; CONTAINS A LARGE AMOUNT OF DARK RED FLUID THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), HEMATO NEOPLASIA (HN) 2888 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1284 ANIMAL NUMBER: C91285 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 202.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:06 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 6.5999 ------------------------------------------- ------------------------------- 3.2560 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2889 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1285 ANIMAL NUMBER: C91285 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 202.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:06 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2890 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1286 ANIMAL NUMBER: C91286 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 381.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:27 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK RED, SLIGHTLY RAISED AREA, 3 X 3 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2891 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1287 ANIMAL NUMBER: C91286 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 381.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:27 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), LUNG (LU), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) 2892 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1288 ANIMAL NUMBER: C91287 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 168.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:52 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 5.8456 ------------------------------------------- ------------------------------- 3.4713 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2893 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1289 ANIMAL NUMBER: C91287 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 168.4 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:52 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2894 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1290 ANIMAL NUMBER: C91288 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 261.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:26 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0295 .0332 1.8834 1.2385 1.2662 8.8606 1.1983 .0327 .0481 .4713 .0092 .0089 .8430 .0113 % .0127 % .7191 % .4729 % .4835 % 3.3832 % .4575 % .0125 % .0184 % .1800 % .0035 % .0034 % .3219 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0157 .0176 1.0000 .6576 .6723 4.7046 .6362 .0174 .0255 .2502 .0049 .0047 .4476 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE PINPOINT AREA UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2895 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1291 ANIMAL NUMBER: C91288 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 261.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:26 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV) 2896 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1292 ANIMAL NUMBER: C91289 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 224.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 13:55 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; ORAL; CLEAR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; COLD TO TOUCH; LIMBSALL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 10 X 10 MM, RED, DARK RED, BROWN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2897 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1293 ANIMAL NUMBER: C91289 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 224.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 13:55 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-ORAL; CLEAR AND MOIST -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL RECTUM (RE) : -PARASITISM,-PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2898 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1294 ANIMAL NUMBER: C91289 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 224.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 13:55 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2899 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1295 ANIMAL NUMBER: C91290 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 294.0 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 13:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 52; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 8 X 5 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -TELANGIECTASIS,-SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, TUBULAR,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL 2900 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1296 ANIMAL NUMBER: C91290 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 294.0 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 13:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 24 X 14 X 10 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED -MALOCCLUSION; BILATERAL: UPPER INCISORS MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2901 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1297 ANIMAL NUMBER: C91290 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/08/99 STUDY DAY OF DEATH: 407 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: 294.0 GRAMS DATE AND TIME OF NECROPSY: 03/08/99 13:28 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2902 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1298 ANIMAL NUMBER: C91291 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 207.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:11 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 7.1834 ------------------------------------------- ------------------------------- 3.4569 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2903 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1299 ANIMAL NUMBER: C91291 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 207.8 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:11 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2904 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1300 ANIMAL NUMBER: C91292 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:57 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER THYROID (TY) : -LARGE; BILATERAL: LEFT IS 7 X 5 X 5 MM, RIGHT IS 7 X 4 X 3 MM PITUITARY (PI) : -LARGE; 6 X 5 X 5 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,- 2 NEOPLASMS PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2905 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1301 ANIMAL NUMBER: C91292 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 741.6 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:57 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -ANIMAL OBESE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) 2906 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1302 ANIMAL NUMBER: C91293 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 278.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0309 .0277 2.0350 1.0502 1.0631 8.1243 1.1760 .0804 .0675 .4346 .0134 .0187 .8268 .0111 % .0099 % .7299 % .3767 % .3813 % 2.9140 % .4218 % .0288 % .0242 % .1559 % .0048 % .0067 % .2966 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0152 .0136 1.0000 .5161 .5224 3.9923 .5779 .0395 .0332 .2136 .0066 .0092 .4063 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2907 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1303 ANIMAL NUMBER: C91293 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 278.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2908 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1304 ANIMAL NUMBER: C91294 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 455.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:12 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -LARGE; LEFT: 5 MM IN DIAMETER (RIGHT IS 4 X 3 X 2 MM) PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED, TAN, GRAY, AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 2909 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1305 ANIMAL NUMBER: C91294 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 455.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:12 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -MASS(ES); WITHIN WALL OF RIGHT HORN: SINGLE MASS, 10 MM IN DIAMETER, DARK RED, TAN AND FIRM; ON SECTION EXUDES A DARK RED SEMIFLUID; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) -SEMISOLID MATERIAL; WITHIN LUMEN OF RIGHT HORN: DARK RED SEMISOLID VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: DARK RED MATERIAL LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED AND TAN; COLLECTED CAVITY, ABDOM (PC) : -MASS(ES); FREE FLOATING WITHIN ABDOMINAL CAVITY: SINGLE MASS, 4 X 3 X 2 MM, YELLOW AND FIRM; COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: RED -BONE MARROW SMEAR TAKEN VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL CAVITY, ABDOM (PC) : -NECROSIS, FAT,-PRESENT LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 2910 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1306 ANIMAL NUMBER: C91294 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 455.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:12 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU) 2911 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1307 ANIMAL NUMBER: C91295 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 87; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 99; 50 X 55MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT MEDIAN LOBE: SINGLE TAN AREA, 5 X 3 MM (CAP 20) -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 11 X 8 X 7 MM, RED, DARK RED, TAN, GRAY AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 60 X 35 X 20 MM, RED, DARK RED, TAN, LOBULATED, AND SOFT; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID (CAP 21) REGIONAL LN (AXILLARY) NORMAL (CAP 22) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 2912 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1308 ANIMAL NUMBER: C91295 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 63 X 50 X 25 MM, RED, DARK RED, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, FOCAL 2913 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1309 ANIMAL NUMBER: C91295 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 454.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:00 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB) 2914 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1310 ANIMAL NUMBER: C91296 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 217.3 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:25 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 12 MM, FIRM, RED, DARK RED AND TAN GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2915 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1311 ANIMAL NUMBER: C91296 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 217.3 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:25 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2916 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1312 ANIMAL NUMBER: C91297 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/23/99 12:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 90; 50 X 40; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -DIFFUSELY RED; RIGHT: DARK RED -SMALL; LEFT: 3 X 2 X 2 MM (RIGHT IS 4 X 3 X 3 MM) PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, RED, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2917 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1313 ANIMAL NUMBER: C91297 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/23/99 12:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CERVIX (CV) : -LARGE; 10 MM IN DIAMETER -FIRM; DIFFUSE SUBCUTANEOUS TIS (SQ) : -MASS(ES); RIGHT DORSAL LUMBAR REGION, EXTENDING TO RIGHT LATERAL ABDOMINAL REGION: SINGLE MASS, 70 X 60 X 40 MM, RED, DARK RED, TAN, SOFT, GELATINOUS, AND FRIABLE; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDED A DARK RED, CLOTTED SEMIFLUID; ANIMAL IDENTIFICATION TRANSPONDER FOUND WITHIN MASS; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SKIN (SK) : -M-FIBROSARCOMA,-NEOPLASM PRESENT CERVIX (CV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -FIBROSARCOMA,-PRESENT SUBCUTANEOUS TIS (SQ) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL >NOTE:>FIBROSARCOMA ENTERED UNDER SKIN 2918 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1314 ANIMAL NUMBER: C91297 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 636 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/23/99 12:23 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2919 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1315 ANIMAL NUMBER: C91298 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 328.7 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:39 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 43; 55 X 55MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; LEFT: 7 X 6 X 5 MM (FOR COMPARISON, RIGHT IS 5 X 5 X 3 MM) -CYST(S); LEFT, DIFFUSE: MULTIPLE RED FLUID FILLED CYSTS, 3 MM IN DIAMETER OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR FLUID FILLED CYSTS ON EACH, UP TO 2 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -FOREIGN BODY,-PRESENT -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, FOCAL -INFLAMMATION, GRANULOMATOUS,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT 2920 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1316 ANIMAL NUMBER: C91298 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 328.7 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:39 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 81 X 55 X 24 MM, TAN, RED, LOBULATED, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 6 X 3 X 2 MM; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -ANIMAL THIN MAMMARY, FEMALE (MF) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS FIBROMA UTERUS (UT) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -FIBROMA,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE 2921 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1317 ANIMAL NUMBER: C91298 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 328.7 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:39 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2922 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1318 ANIMAL NUMBER: C91299 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 216.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0346 .0309 1.8673 .9484 .9537 7.0761 1.0849 .0498 .0500 .3660 .0070 .0094 .4825 .0160 % .0143 % .8641 % .4389 % .4413 % 3.2745 % .5020 % .0230 % .0231 % .1694 % .0032 % .0043 % .2233 % .0185 .0165 1.0000 .5079 .5107 3.7895 .5810 .0267 .0268 .1960 .0037 .0050 .2584 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2923 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1319 ANIMAL NUMBER: C91299 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 216.1 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:52 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2924 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1320 ANIMAL NUMBER: C91300 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/27/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -DIFFUSELY RED; ALL LOBES: DARK RED -UNCOLLAPSED; ALL LOBES PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, DARK RED, RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL THORACIC REGION: SINGLE MASS, 10 X 10 X 5 MM, BROWN, TAN AND SOFT; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL LUNG (LU) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2925 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1321 ANIMAL NUMBER: C91300 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/27/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY DARK BROWN MATERIAL -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2926 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1322 ANIMAL NUMBER: C91300 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/27/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2927 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1323 ANIMAL NUMBER: C91301 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:39 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 99; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 105; 20 X 20MM; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ANTERIOR ASPECT: SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER -LARGE; 6 X 6 X 5 MM; APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 60 X 40 X 30 MM, RED, DARK RED, TAN, VASCULAR, LOBULATED AND FIRM; SOME PORTIONS EXUDE A DARK RED SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 2928 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1324 ANIMAL NUMBER: C91301 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:39 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; LEFT PERINEAL REGION: SINGLE MASS, 40 X 30 X 15 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 21); POSSIBLY CONSISTENT WITH THE MASS (INGUINAL- LEFT) NOTED IN THE CLINICAL OBSERVATIONS -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2929 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1325 ANIMAL NUMBER: C91301 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 13:39 PROSECTOR: MICHELE MARGGI RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX) 2930 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1326 ANIMAL NUMBER: C91302 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 218.6 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 14:05 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -LARGE PELVIS(ES); LEFT PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 10 X 9 MM, RED, DARK RED, TAN AND FIRM MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2931 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1327 ANIMAL NUMBER: C91302 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 218.6 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 14:05 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -BLOOD FILM TAKEN -ANIMAL THIN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE 2932 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1328 ANIMAL NUMBER: C91302 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/13/99 STUDY DAY OF DEATH: 504 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 218.6 GRAMS DATE AND TIME OF NECROPSY: 06/13/99 14:05 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2933 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1329 ANIMAL NUMBER: C91303 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:53 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 100; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 105; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 X 3 MM; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, DARK RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL,-MODERATE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SEVERE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-MODERATE, MULTI-FOCAL 2934 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1330 ANIMAL NUMBER: C91303 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:53 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 15 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 20 X 15 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; REGIONAL LN (INGUINAL) NORMAL (CAP 22) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -GI TRACT-DARK FLUID CONTENT; STOMACH DUODENUM AND JEJUNUM: DARK BROWN SEMIFLUID MATERIAL -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2935 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1331 ANIMAL NUMBER: C91303 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 589.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 10:53 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, AXILLARY (AX), LN, INGUINAL (IN) 2936 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1332 ANIMAL NUMBER: C91304 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 300.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0421 .0424 2.1378 1.2554 1.2507 10.4193 1.2114 .0627 .0679 .4924 .0094 .0130 .6986 .0140 % .0141 % .7107 % .4174 % .4158 % 3.4639 % .4027 % .0208 % .0226 % .1637 % .0031 % .0043 % .2322 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0197 .0198 1.0000 .5872 .5850 4.8738 .5667 .0293 .0318 .2303 .0044 .0061 .3268 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED SKIN (SK) : -ALOPECIA-FOCAL; CERVICAL REGION: TWO AREAS, ONE IS 25 X 21 MM AND THE OTHER IS 15 X 10 MM (CAP 20) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2937 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1333 ANIMAL NUMBER: C91304 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 300.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 10:23 PROSECTOR: GREGORY CODDINGTON RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 2938 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1334 ANIMAL NUMBER: C91305 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 557.0 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:24 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; PARALYSIS; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); SINGLE MASS, 4 X 5 X 9 MM, FIRM, LOBULATED, RED, DARK RED AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 2939 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1335 ANIMAL NUMBER: C91305 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 557.0 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:24 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 12 X 14 X 21 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MATERIAL -BLOOD FILM TAKEN MOIST CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2940 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1336 ANIMAL NUMBER: C91305 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 604 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 557.0 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 10:24 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2941 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1337 ANIMAL NUMBER: C91306 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 455.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 74; 60 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 85; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 92; 35 X 25MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 93; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, BROWN, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : >UNREMARKABLE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -EDEMA,-MODERATE, MULTI-FOCAL 2942 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1338 ANIMAL NUMBER: C91306 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 455.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 17 X 15 X 15 MM, DARK RED, BROWN, TAN AND SOFT; SAME ON CUT SURFACE; (CAP 21); REGIONAL LN (SEE AXILLARY) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 90 X 70 X 40 MM, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED CRUSTED AREA, 40 X 30 MM, COLLECTED INTACT -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 20 X 17 X 17 MM, DARK RED, BROWN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 26) -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 45 X 40 X 30 MM, TAN, BROWN, AND FIRM; CUT SURFACE CONTAINS A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 26) UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE SKIN (SK) : -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL -NECROSIS,-MODERATELY SEVERE, MULTI FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,- 3 NEOPLASMS PRESENT UTERUS (UT) : >UNREMARKABLE 2943 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1339 ANIMAL NUMBER: C91306 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 455.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 15 X 10 X 5 MM (RIGHT IS UP TO 9 X 5 X 3 MM) (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL 2944 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1340 ANIMAL NUMBER: C91306 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 455.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: AS ASSIGNED THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, INGUINAL (IN) 2945 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1341 ANIMAL NUMBER: C91307 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 394.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, RED, DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LN, MESENTERIC (MS) : >UNREMARKABLE LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2946 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1342 ANIMAL NUMBER: C91307 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 394.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:15 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) 2947 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1343 ANIMAL NUMBER: C91308 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 319.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 8:51 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -MOTTLED; GREEN AND TAN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT 2948 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1344 ANIMAL NUMBER: C91308 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 319.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 8:51 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 17 X 10 X 8 MM, DARK RED, GRAY, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2949 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1345 ANIMAL NUMBER: C91308 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/20/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 319.3 GRAMS DATE AND TIME OF NECROPSY: 05/20/99 8:51 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2950 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1346 ANIMAL NUMBER: C91309 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 466.4 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 57; 40 X 45MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL; WEEK 62; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SPLEEN (SP) : -LARGE; 60 X 15 X 10 MM PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK GREY FOCUS, 1 MM IN DIAMETER -LARGE; 5 X 5 X 3 MM, APPEARS SWOLLEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2951 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1347 ANIMAL NUMBER: C91309 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 466.4 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 45 X 40 X 25 MM, RED, DARK RED, TAN, SOFT AND LOBULATED; ON SECTION EXUDED A BROWN AND TAN CASEOUS SEMISOLID MATERIAL (COLLECTED); OVERLYING SKIN HAS MULTIPLE ULCERATED AREAS, UP TO 20 MM IN DIAMETER (COLLECTED INTACT WITH MASS); REGIONAL LN (INGUINAL) NORMAL (CAP 20) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 MM IN DIAMETER (CAP 21) -MASS #2; LEFT SHOULDER REGION: SINGLE MASS, 50 X 20 X 15 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (COLLECTED); REGIONAL LN (SEE AXILLARY) UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 8 X 5 X 5 MM LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 15 X 8 X 3 MM (FOR COMPARISON, RIGHT IS UP TO 5 X 3 X 1 MM) (CAP 22) MAMMARY, FEMALE (MF) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -NECROSIS,-SLIGHT, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL 2952 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1348 ANIMAL NUMBER: C91309 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/04/99 STUDY DAY OF DEATH: 464 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 466.4 GRAMS DATE AND TIME OF NECROPSY: 05/04/99 14:35 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2953 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1349 ANIMAL NUMBER: C91310 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 500.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 71; 80 X 95MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) -LARGE; 6 X 6 X 3 MM, APPEARS SWOLLEN MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 100 X 75 X 30 MM, RED, DARK RED, TAN, LOBULATED, AND VASCULAR; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) HISTOPATHOLOGY LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-MODERATE, MULTI-FOCAL -NECROSIS,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : >TISSUE MISSING 2954 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1350 ANIMAL NUMBER: C91310 SEX: FEMALE DOSE GROUP: 3 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 500.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 9:43 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), URINARY BLADDER (UB) 2955 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-212 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e V III o f X II Page 2956 of 4873 Covance 6329-212 3M T-6316.1 VOLUME VIII OF XII CONTENTS Page A P P E N D I X 6 ( C o n t i n u e d ) .................................................................................................................................................... 2 9 5 8 I n d i v i d u a l A n a t o m i c P a t h o l o g y D a t a .................................................................................................................... 2 9 5 9 A P P E N D I X 7 ...................................................................................................................................................................................... 3 2 9 9 C e l l P r o l i f e r a t i o n R e p o r t ...................................................................................................................................................3 3 0 0 A P P E N D I X 8 ...................................................................................................................................................................................... 3 3 3 8 E l e c t r o n M i c r o s c o p y R e p o r t ........................................................................................................................................ 3 3 3 9 2957 APPENDIX 6 (Continued) In d iv id u a l A n a to m ic P a th o lo g y D a ta Covance 6329-212 3M T-6316.1 2958 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1351 ANIMAL NUMBER: C91311 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 444 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/14/99 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AREA, 3 X 2 MM; COLLECTED ON ROUTINE SECTION -MOTTLED; ALL LOBES: DARK BROWN AND TAN OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 9 MM IN DIAMETER, RIGHT IS 4 MM IN DIAMETER PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN, PINPOINT FOCI UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: DARK RED FLUID HISTOPATHOLOGY LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE UTERUS (UT) : -DILATATION,-SEVERE, DIFFUSE -HEMORRHAGE,-MODERATELY SEVERE, DIFFUSE 2959 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1352 ANIMAL NUMBER: C91311 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 444 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/14/99 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -LARGE, DIFFUSE; BILATERAL: EACH IS UP TO 15 MM IN DIAMETER CAUSE OF DEATH (CD) : -UTERINE HEMORRHAGE,-PRESENT 2960 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1353 ANIMAL NUMBER: C91311 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/14/99 STUDY DAY OF DEATH: 444 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/14/99 10:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2961 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1354 ANIMAL NUMBER: C91312 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 200.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; GENITAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, RED, DARK RED, GREY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL 2962 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1355 ANIMAL NUMBER: C91312 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 200.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND RED -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -ANIMAL THIN SALIV GL, MANDIB (SG) : >TISSUE MISSING STOMACH, GL (ST) : -EDEMA,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2963 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1356 ANIMAL NUMBER: C91312 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 200.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:47 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM(JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB),CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN),TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM(JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2964 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1357 ANIMAL NUMBER: C91313 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 357.8 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 8:40 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 71; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 74; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRALTHORACIC; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 85; 25 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 85; 45 X 45MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 13 MM, FIRM, RED, DARK RED AND TAN MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 6 X 9 X 13 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (AXILLARY) NORMAL (CAP 22) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL THYMUS (TH) : -M-THYMIC CARCINOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,- 4 NEOPLASMS PRESENT 2965 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1358 ANIMAL NUMBER: C91313 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 357.8 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 8:40 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 13 X 15 X 30 MM, FIRM, RED, DARK BROWN, TAN AND LOBULATED; ON SECTION EXUDES A DARK BROWN SEMIFLUID (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 20 X 36 X 45 MM, FIRM, RED, DARK RED, TAN, BROWN AND LOBULATED; ON SECTION EXUDES A BROWN FLUID (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #4; LEFT PERIANAL REGION: SINGLE MASS, 25 X 45 X 55 MM, FIRM, RED, DARK RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -THYMIC CARCINOMA,-PRESENT 2966 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1359 ANIMAL NUMBER: C91313 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 357.8 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 8:40 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 2967 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1360 ANIMAL NUMBER: C91314 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 314.2 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:33 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 61; 35 X 35MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, EACH IS 6 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 7 X 6 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MINIMAL, FOCAL OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -THROMBUS,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 2968 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1361 ANIMAL NUMBER: C91314 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 314.2 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:33 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT CERVICAL REGION, EXTENDS TO RIGHT AXILLARY REGION: SINGLE MASS, 58 X 37 X 26 MM, RED, DARK RED, TAN, BROWN, FIRM AND LOBULATED; ON SECTION, SOME AREAS EXUDE A DARK BROWN FLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) CERVIX (CV) : -LARGE; 8 X 6 X 5 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY RED MATERIAL -ANIMAL THIN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT CERVIX (CV) : >UNREMARKABLE CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE 2969 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1362 ANIMAL NUMBER: C91314 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 314.2 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 13:33 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) 2970 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1363 ANIMAL NUMBER: C91315 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 99; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 103; 50 X 40MM SIDE RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL AND RIGHT MEDIAN LOBES: MULTIPLE TAN AREAS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS -MASS(ES); PAPILLARY PROCESS: SINGLE MASS, 15 X 12 X 8 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (HEPATIC) NOT IDENTIFIED -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS -MASS #2; RIGHT LATERAL LOBE: SINGLE MASS, 15 X 15 X 7 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (HEPATIC) NOT IDENTIFIED HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,- 2 NEOPLASMS PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL 2971 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1364 ANIMAL NUMBER: C91315 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -MASS(ES); DORSAL LUMBAR REGION: SINGLE MASS, 15 X 15 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 23); ANIMAL IDENTIFICATION TRANSPONDER FOUND WITHIN MASS; REGIONAL LN (SEE INGUINAL) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 70 X 60 X 30 MM, RED, DARK RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE INGUINAL) CERVIX (CV) : -LIGHT FOCUS(I)/AREA(S); TRIMMING COMMENT: WITHIN LUMEN: SINGLE TAN AREA, 3 X 3 X 2 MM; TRIMMED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -THROMBUS,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT SKIN (SK) : -FIBROSIS,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL -ULCERATION,-SEVERE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CERVIX (CV) : -DILATATION,-MODERATE, FOCAL -B-LEIOMYOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 2972 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1365 ANIMAL NUMBER: C91315 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED LN, INGUINAL (IN) : -DIFFUSELY DARK; LEFT: BROWN (RIGHT IS NORMAL) (CAP 22) PINNA (PN) : -RED FOCUS(I)/AREA(S); BILATERAL, DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 5 X 2 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, INGUINAL (IN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL PINNA (PN) : -HEMORRHAGE,-SLIGHT, FOCAL 2973 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1366 ANIMAL NUMBER: C91315 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.7 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN) 2974 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1367 ANIMAL NUMBER: C91316 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 193.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 6.6687 ------------------------------------------- ------------------------------- 3.4499 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2975 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1368 ANIMAL NUMBER: C91316 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 193.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2976 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1369 ANIMAL NUMBER: C91317 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:57 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 7.3592 ------------------------------------------- ------------------------------- 3.4599 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 2977 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1370 ANIMAL NUMBER: C91317 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:57 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 2978 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1371 ANIMAL NUMBER: C91318 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 05/07/98 STUDY DAY OF DEATH: 102 STUDY WEEK OF DEATH: 15 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/98 14:41 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -LACERATION; DIFFUSE: MULTIPLE LACERATIONS; TISSUE IS DARK RED, GELATINOUS AND AMORPHIC LUNG (LU) : -MOTTLED; ALL LOBES: RED AND DARK RED -UNCOLLAPSED; ALL LOBES PITUITARY (PI) : -MISSING/NOT IDENTIFIED; NOT IDENTIFIED DUE TO DIFFUSE FRACTURES OF CALVARIUM HARDERIAN GLAND (HG) : >NOTE:>TRIM COMMENT: NOT FOUND AT TIME OF TRIM CALVARIUM/SKULL (CA) : -FRACTURE(S); DIFFUSE: MULTIPLE FRACTURES; COLLECTED INTACT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK RED FLUID -DISCHARGE-NASAL; DARK RED FLUID HISTOPATHOLOGY BRAIN (BR) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL LUNG (LU) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : >TISSUE MISSING HARDERIAN GLAND (HG) : >TISSUE MISSING CAUSE OF DEATH (CD) : -FRACTURE, CALVARIUM,-PRESENT CALVARIUM/SKULL (CA) : -FRACTURE,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL 2979 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1372 ANIMAL NUMBER: C91318 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 05/07/98 STUDY DAY OF DEATH: 102 STUDY WEEK OF DEATH: 15 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/98 14:41 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; HEAD: DARK RED, MOIST MATERIAL -TRACHEA-CONTENT FOAMY MATTER; DARK RED, FOAMY MATERIAL 2980 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1373 ANIMAL NUMBER: C91318 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (A) DATE OF DEATH: 05/07/98 STUDY DAY OF DEATH: 102 STUDY WEEK OF DEATH: 15 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/07/98 14:41 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA) 2981 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1374 ANIMAL NUMBER: C91319 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 274.1 GRAMS DATE AND TIME OF NECROPSY: 05/26/99 14:11 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; ORAL; CLEAR; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK RED, GREY, LOBULATED AND SOFT GENERAL COMMENT (GC) : -DISCHARGE-ORAL; MOIST, CLEAR MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2982 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1375 ANIMAL NUMBER: C91319 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/26/99 STUDY DAY OF DEATH: 486 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 274.1 GRAMS DATE AND TIME OF NECROPSY: 05/26/99 14:11 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2983 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1376 ANIMAL NUMBER: C91320 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:30 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: SINGLE TAN AREA, 9 X 2 MM; COLLECTED ON ROUTINE SECTION -MOTTLED; ALL LOBES, DIFFUSE: BROWN AND TAN KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN ADRENAL, CORTEX (AC) : -LIGHT FOCUS(I)/AREA(S); BILATERAL: MULTIPLE TAN, PINPOINT FOCI -DIFFUSELY DARK; BILATERAL: BROWN PITUITARY (PI) : -MASS(ES); SINGLE MASS, 8 X 6 X 5 MM, RED, DARK RED, TAN, SOFT AND LOBULATED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 2984 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1377 ANIMAL NUMBER: C91320 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:30 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2985 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1378 ANIMAL NUMBER: C91320 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 314.7 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:30 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2986 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1379 ANIMAL NUMBER: C91321 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/10/99 STUDY DAY OF DEATH: 654 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 406.7 GRAMS DATE AND TIME OF NECROPSY: 11/10/99 14:59 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 90; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; LUMBAR-LEFT; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 10 X 7 MM, RED, DARK RED, GREY, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL -FIBROSIS,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL 2987 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1380 ANIMAL NUMBER: C91321 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/10/99 STUDY DAY OF DEATH: 654 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 406.7 GRAMS DATE AND TIME OF NECROPSY: 11/10/99 14:59 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 22 X 20 X 10 MM, TAN, RED, AND SOFT; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A SINGLE TAN ULCERATED AREA; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (DORSAL) NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2988 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1381 ANIMAL NUMBER: C91321 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/10/99 STUDY DAY OF DEATH: 654 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 406.7 GRAMS DATE AND TIME OF NECROPSY: 11/10/99 14:59 PROSECTOR: TODD ACKER RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2989 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1382 ANIMAL NUMBER: C91322 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/25/98 STUDY DAY OF DEATH: 151 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: 275.1 GRAMS DATE AND TIME OF NECROPSY: 06/25/98 9:20 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOWISHBROWN -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -DISCHARGE-NASAL; BILATERAL: DARK RED AND DRY MATERIAL -MALOCCLUSION; BILATERAL, UPPER INCISORS HISTOPATHOLOGY OVARY (OV) : -MINERALIZATION,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 2990 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1383 ANIMAL NUMBER: C91322 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/25/98 STUDY DAY OF DEATH: 151 STUDY WEEK OF DEATH: 22 TERMINAL BODY WEIGHT: 275.1 GRAMS DATE AND TIME OF NECROPSY: 06/25/98 9:20 PROSECTOR: KEVIN BILLINGS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) (JE), 2991 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1384 ANIMAL NUMBER: C91323 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 343.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0385 .0328 2.3182 1.3772 1.3727 19.1752 1.7596 .0546 .0493 .4801 .0143 .0102 1.1650 .0112 % .0096 % .6759 % .4015 % .4002 % 5.5904 % .5130 % .0159 % .0144 % .1400 % .0042 % .0030 % .3397 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0166 .0141 1.0000 .5941 .5921 8.2716 .7590 .0236 .0213 .2071 .0062 .0044 .5025 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 51; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR.-MODERATE. MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL OVARY (OV) : -HEMORRHAGE,-MINIMAL, FOCAL 2992 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1385 ANIMAL NUMBER: C91323 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 343.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 1 MM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 25 X 17 X 10 MM, RED, DARK RED, TAN, LOBULATED, CYSTIC AND FIRM; SOME SECTIONS EXUDE A DARK RED FLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS; COLLECTED ON ROUTINE SECTION THYMUS (TH) : -FIBROSIS,-MODERATE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT, FOCAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION 2993 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1386 ANIMAL NUMBER: C91323 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 343.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: DONIA MEYER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 2994 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1387 ANIMAL NUMBER: C91324 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 316.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:10 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN -DARK FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK BROWN AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS THYMUS (TH) : -CYST(S); AT BIFURCATION OF LOBES: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER; COLLECTED PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN HISTOPATHOLOGY LIVER (LI) : -TELANGIECTASIS,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 2995 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1388 ANIMAL NUMBER: C91324 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 316.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:10 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -THICKENED WALL; BILATERAL: DIFFUSE FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE RED, DRY, CRUSTED AREA, 3 X 2 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -INFLAMMATION, CHRONIC ACTIVE,-SLIGHT, FOCAL 2996 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1389 ANIMAL NUMBER: C91324 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 316.0 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:10 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 2997 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1390 ANIMAL NUMBER: C91325 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:06 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADCRANIAL; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: DARK BROWN AND TAN KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: DARK BROWN ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 8 X 8 X 7 MM (LEFT IS 5 X 4 X 4 MM) LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 5 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -VACUOLATION,-MODERATELY SEVERE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 2998 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1391 ANIMAL NUMBER: C91325 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:06 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY THYROID (TY) : -LARGE; RIGHT: 7 X 5 X 5 MM (LEFT IS 5 X 4 X 4 MM) PITUITARY (PI) : -MASS(ES); 13 X 11 X 7 MM; DARK RED, TAN AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 2999 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1392 ANIMAL NUMBER: C91325 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 14:06 PROSECTOR: WENDY CHERRY RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) (SG), 3000 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1393 ANIMAL NUMBER: C91326 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; LIMBSFRONT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN PITUITARY (PI) : -MASS(ES); 10 X 7 X 7 MM, RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT CAVITY, ABDOM (PC) : -NECROSIS, FAT,-PRESENT 3001 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1394 ANIMAL NUMBER: C91326 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE NEAR RIGHT OVARY: SINGLE MASS, 8 X 3 X 2 MM, YELLOW AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT WITH OVARY; REGIONAL LN (ANTERIOR MESENTERIC) NOT IDENTIFIED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK YELLOW -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; RED -ANIMAL THIN HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT 3002 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1395 ANIMAL NUMBER: C91326 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 212.7 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:48 PROSECTOR: TERRI L. WILLIAMS RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN) 3003 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1396 ANIMAL NUMBER: C91327 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 84; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ON MARGIN OF LEFT MEDIAN LOBE: SINGLE RED AREA, 8 X 7 MM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, FOCAL -TELANGIECTASIS,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, CORTICAL,-MODERATELY SEVERE, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3004 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1397 ANIMAL NUMBER: C91327 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 40 X 25 X 15 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A DARK BROWN FLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN LUMEN: SINGLE BROWN FLUID FILLED CYST, 10 X 3 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3005 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1398 ANIMAL NUMBER: C91327 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 493.2 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:45 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3006 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1399 ANIMAL NUMBER: C91328 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 437 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 336.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 13:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; ORAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; DORSALCERVICAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID POSTERIOR ASPECT: SINGLE DARK BROWN FOCUS, 2 MM IN DIAMETER UTERUS (UT) : -LUMEN FILLED WITH FLUID; RIGHT: CLEAR FLUID LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN; COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DRY, RED MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL HISTOPATHOLOGY ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 3007 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1400 ANIMAL NUMBER: C91328 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 437 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 336.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 13:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND HEAD REGION: DRY, RED MATERIAL 3008 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1401 ANIMAL NUMBER: C91328 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 437 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 336.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 13:55 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3009 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1402 ANIMAL NUMBER: C91329 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:56 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0247 .0268 2.0389 .9396 .9792 9.5614 1.1670 .0495 .0478 .3983 .0098 .0122 .4650 .0108 % .0117 % .8884 % .4094 % .4267 % 4.1662 % .5085 % .0216 % .0208 % .1736 % .0043 % .0053 % .2026 % .0121 .0131 1.0000 .4608 .4803 4.6895 .5724 .0243 .0234 .1954 .0048 .0060 .2281 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3010 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1403 ANIMAL NUMBER: C91329 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.5 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:56 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL INFORMATION (XX) : >NOTE:>ANIMAL DIED FOLLOWING SAMPLE COLLECTION AND PRIOR TO ANESTHESIA; NO ANESTHESIA ADMINISTERED THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3011 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1404 ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC; WEEK 54; 50 X 45MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 68; 35 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL-CERVICAL-LEFT; WEEK 80; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; SACRAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 84; 15 X 15MM ANTERIOR; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 85; 20 X 20 MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 85; 20 X 20MM POSTERIOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -LARGE; 57 X 14 X 7 MM OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, RED, TAN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL,-MODERATE, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -MINERALIZATION,-MODERATE, FOCAL 3012 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1405 ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 20 X 15 X 14 MM, DARK RED, GRAY, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT POSTERIOR) AS NOTED IN CLINICAL OBSERVATIONS -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 30 X 25 X 15 MM, DARK RED, RED, TAN, GRAY AND FIRM; SAME ON CUT SURFACE; ON SECTION EXUDES A BROWN FLUID (CAP 25); REGIONAL LN (SEE AXILLARY); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT) AS NOTED IN CLINICAL OBSERVATIONS -MASS #3; MID VENTRAL CERVICAL REGION: SINGLE MASS, 50 X 35 X 12 MM, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 26); REGIONAL LN (MANDIBULAR) NORMAL (CAP 22); POSSIBLY CONSISTENT WITH MASS (VENTRAL THORACIC) AS NOTED IN CLINICAL OBSERVATIONS MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,- 5 NEOPLASMS PRESENT 3013 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1406 ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #4; LEFT LATERAL CERVICAL REGION: SINGLE MASS, 35 X 30 X 20 MM, DARK BROWN, BROWN, RED, LOBULATED AND SOFT; SAME ON CUT SURFACE (CAP 27); REGIONAL LN (MANDIBULAR) NORMAL (CAP 22); POSSIBLY CONSISTENT WITH MASS (DORSAL CERVICAL LEFT) AS NOTED IN CLINICAL OBSERVATIONS -MASS #5; DORSAL CERVICAL REGION: SINGLE MASS, 30 X 20 X 20 MM, TAN, RED, DARK RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; ON SECTION EXUDES A BROWN SEMIFLUID MATERIAL (CAP 28); REGIONAL LN NORMAL (CAP 22); POSSIBLY CONSISTENT WITH MASS (DORSAL) AS NOTED IN CLINICAL OBSERVATIONS -MASS #6; PERIANAL REGION: TWO MASSES, EACH IS 20 X 15 X 10, BOTH ARE TAN, DARK RED, LOBULATED AND FIRM; BOTH ARE SAME ON CUT SURFACE (CAP 29); REGIONAL LN (SEE INGUINAL); POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; OVERLYING SKIN IS DARK BROWN, MOIST AND ULCERATED, 12 MM IN DIAMETER; COLLECTED INTACT WITH MASS 3014 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1407 ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER -THICKENED WALL; BILATERAL, DIFFUSE LN, AXILLARY (AX) : -LARGE; LEFT: UP TO 7 X 5 X 2 MM (RIGHT IS UP TO 3 X 2 X 1 MM) (CAP 23) LN, INGUINAL (IN) : -LARGE; BILATERAL: EACH IS UP TO 4 X 4 MM (CAP 21) -DIFFUSELY DARK; LEFT: DARK BROWN (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY CARCINOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL LN, AXILLARY (AX) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, MULTI-FOCAL LN, INGUINAL (IN) : -N-MAMMARY CARCINOMA,-NEOPLASM PRESENT 3015 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1408 ANIMAL NUMBER: C91330 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/07/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 406.5 GRAMS DATE AND TIME OF NECROPSY: 09/07/99 14:41 PROSECTOR: JERALD W. JOHNSON RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3016 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1409 ANIMAL NUMBER: C91331 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 256.8 GRAMS DATE AND TIME OF NECROPSY: 07/20/99 13:58 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LIGHT FOCUS(I)/AREA(S); LEFT CORTEX: SINGLE TAN AREA, 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -CYST,-PRESENT 3017 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1410 ANIMAL NUMBER: C91331 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 256.8 GRAMS DATE AND TIME OF NECROPSY: 07/20/99 13:58 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 9 X 5 MM, RED, DARK RED, GRAY, TAN, CYSTIC, AND SOFT UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: YELLOW AND MOIST -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: RED AND MOIST -ANIMAL THIN THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3018 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1411 ANIMAL NUMBER: C91331 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/20/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: 256.8 GRAMS DATE AND TIME OF NECROPSY: 07/20/99 13:58 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3019 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1412 ANIMAL NUMBER: C91332 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 306.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:16 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, DARK RED, TAN, GREY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3020 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1413 ANIMAL NUMBER: C91332 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 306.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:16 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, ANT MES/PANC (AP) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>PANCREATIC LYMPH NODE WAS PRESENT ON PANCREAS SLIDE 3021 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1414 ANIMAL NUMBER: C91332 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 306.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:16 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3022 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1415 ANIMAL NUMBER: C91333 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 7.5152 ------------------------------------------- ------------------------------- 4.0188 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3023 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1416 ANIMAL NUMBER: C91333 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 10:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 3024 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1417 ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 86; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 97; 60 X 60MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -MASS(ES); INVOLVING ENTIRE LEFT OVARY: SINGLE MASS, 25 X 14 X 14 MM, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (RENAL) NORMAL (CAP 22) -MASS #2; ATTACHED TO MASS #1: SINGLE MASS, 40 X 30 X 25 MM, DARK GREEN, BROWN, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (RENAL) NORMAL (CAP 22) HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, MULTI-FOCAL -ULCERATION, CORNEAL,-MODERATE, FOCAL -VASCULARIZATION, CORNEAL,-MODERATE, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MODERATELY SEVERE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -ONE EXAMINED,-PRESENT >NOTE:>OVARIAN MASS DESCRIBED GROSSLY WAS NECROTIC MESENTERIC FAT 3025 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1418 ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY HEART (HT) : -DIFFUSELY LIGHT; TAN PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AND BROWN AREAS, UP TO 3 X 1 MM THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -MINERALIZATION,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MODERATELY SEVERE, MULTI-FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL -B-ADENOMA,- 2 NEOPLASMS PRESENT 3026 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1419 ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 35 X 20 X 10 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DARK RED AND DRY CRUSTED AREA, 7 X 4 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 65 X 55 X 25 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED CAUSE OF DEATH (CD) : -RENAL MINERALIZATION,-PRESENT CAVITY, ABDOM (PC) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -NECROSIS, FAT,-PRESENT -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MODERATELY SEVERE, MULTI FOCAL LN, RENAL (RL) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3027 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1420 ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE LN, RENAL (RL) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 3028 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1421 ANIMAL NUMBER: C91334 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/31/99 STUDY DAY OF DEATH: 705 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/31/99 8:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3029 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1422 ANIMAL NUMBER: C91335 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 395.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:52 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AND SLIGHTLY DEPRESSED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE TAN AREAS, PINPOINT AND UP TO 1 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MINIMAL, DIFFUSE -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 3030 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1423 ANIMAL NUMBER: C91335 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 395.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:52 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3031 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1424 ANIMAL NUMBER: C91335 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 395.1 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:52 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3032 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1425 ANIMAL NUMBER: C91336 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 335.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 88; 10 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -THROMBUS,-MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT 3033 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1426 ANIMAL NUMBER: C91336 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 335.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY THYROID (TY) : -MISSING/NOT IDENTIFIED; LEFT: MISSING PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); MID PECTORAL REGION: SINGLE MASS, 22 X 17 X 10 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (SEE AXILLARY) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 25 X 20 X 10 MM, RED, DARK RED, TAN AND FIRM; CUT SURFACE EXUDES A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #3; LEFT PERINEAL REGION: SINGLE MASS, 28 X 20 X 15 MM, DARK RED, TAN AND FIRM; CUT SURFACE EXUDES A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 21) THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -ONE EXAMINED,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,- 2 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 3034 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1427 ANIMAL NUMBER: C91336 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 335.7 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:10 PROSECTOR: TODD ACKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3035 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1428 ANIMAL NUMBER: C91337 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 262.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:38 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0311 .0318 1.9778 1.1313 1.1432 11.4262 1.0703 .0514 .0622 .4922 .0106 .0102 .5673 .0118 % .0121 % .7526 % .4305 % .4350 % 4.3479 % .4073 % .0196 % .0237 % .1873 % .0040 % .0039 % .2159 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0157 .0161 1.0000 .5720 .5780 5.7772 .5412 .0260 .0314 .2489 .0054 .0052 .2868 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3036 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1429 ANIMAL NUMBER: C91337 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 262.8 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:38 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3037 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1430 ANIMAL NUMBER: C91338 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:26 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS CERVIX (CV) : -MASS(ES); EXTENDING INTO LUMEN OF BOTH UTERINE HORNS: SINGLE MASS, 30 X 10 X 9 MM, RED, DARK RED, TAN, GRAY, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) HISTOPATHOLOGY LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CYST,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL CERVIX (CV) : -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, FOCAL 3038 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1431 ANIMAL NUMBER: C91338 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:26 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: BROWN FLUID -MASS(ES); WITHIN LUMEN OF LEFT HORN: SINGLE MASS, 25 X 20 X 10 MM, TAN, RED, LOBULATED AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3039 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1432 ANIMAL NUMBER: C91338 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 310.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 11:26 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 3040 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1433 ANIMAL NUMBER: C91339 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : >NOTE:>TRIMMING COMMENT: ONE EYE MISSING BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DARK FOCUS(I)/AREA(S); RIGHT MEDIAN AND LEFT LATERAL LOBES: MULTIPLE DARK BROWN AREAS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -CYST(S); RIGHT, CORTICAL SURFACE: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY EYE (EY) : -ONE EXAMINED,-PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -CYST,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -THROMBUS,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3041 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1434 ANIMAL NUMBER: C91339 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE BROWN ULCERATED AREA, 5 MM IN DIAMETER MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND SOFT; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY DARK RED MATERIAL -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: DRY DARK RED MATERIAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL MAMMARY, FEMALE (MF) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3042 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1435 ANIMAL NUMBER: C91339 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:52 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3043 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1436 ANIMAL NUMBER: C91340 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 286.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:29 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 67; 60 X 55MM APPEARS TO HAVE MIGRATED FROM VENTRAL THORACIC; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN OVARY (OV) : -CYST(S); RIGHT: TWO CLEAR FLUID FILLED CYSTS, EACH IS 7 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED, TAN, GRAY, AND SOFT HISTOPATHOLOGY EYE (EY) : -VASCULARIZATION, CORNEAL,-SLIGHT, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, CORTICAL,-MODERATE, FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MODERATE, MULTI-FOCAL 3044 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1437 ANIMAL NUMBER: C91340 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 286.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:29 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 4 X 2 MM SKIN (SK) : >NOTE:>COLLECTED ON RIGHT SIDE DUE TO MAMMARY GLAND MASS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 55 X 45 X 17 MM, TAN, GRAY, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; RIGHT INGUINAL REGION: TAN AND FIRM; COLLECTED ON ROUTINE SECTION >NOTE:>COLLECTED ON RIGHT SIDE DUE TO MAMMARY GLAND MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED, DRY MATERIAL -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT VAGINA (VA) : >TISSUE MISSING CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3045 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1438 ANIMAL NUMBER: C91340 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 286.4 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:29 PROSECTOR: JERALD W. JOHNSON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) (SM), 3046 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1439 ANIMAL NUMBER: C91341 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 426.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 73; 60 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LARGE; 6 X 5 X 4 ; APPEARS SWOLLEN HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL LIVER, OIL RED O (LI1) : -POSITIVE OIL RED O STAINING,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : >UNREMARKABLE HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL 3047 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1440 ANIMAL NUMBER: C91341 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 426.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; VENTRAL CERVICAL REGION: SINGLE MASS, 90 X 45 X 30 MM, RED, DARK RED, TAN, FIRM, VASCULAR AND LOBULATED; SOME SECTIONS EXUDE A DARK GREEN SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED AND MOIST CRUSTED AREA, COLLECTED INTACT WITH MASS LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 9 X 4 X 3 MM (CAP 20) GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN SKIN (SK) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -ULCERATION,-SEVERE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-SLIGHT, MULTI FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL 3048 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1441 ANIMAL NUMBER: C91341 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 426.3 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 11:28 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG),STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT),MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG),STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3049 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1442 ANIMAL NUMBER: C91342 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED, TAN, AND BROWN LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 10 X 10 MM HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3050 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1443 ANIMAL NUMBER: C91342 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM CERVIX (CV) : -LARGE; 9 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THYROID (TY) : -B-ADENOMA, C-CELL,- 2 NEOPLASMS PRESENT PITUITARY (PI) : >UNREMARKABLE CERVIX (CV) : ^UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE 3051 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1444 ANIMAL NUMBER: C91342 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 430.5 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:25 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER(JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3052 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1445 ANIMAL NUMBER: C91343 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:03 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0457 .0379 1.9894 1.4136 1.4822 13.1212 1.7758 .0724 .0606 .6191 .0103 .0193 1.0097 .0168 % .0139 % .7293 % .5182 % .5433 % 4.8098 % .6510 % .0265 % .0222 % .2269 % .0038 % .0071 % .3701 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0230 .0191 1.0000 .7106 .7450 6.5956 .8926 .0364 .0305 .3112 .0052 .0097 .5075 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -STASIS, BILE,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL 3053 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1446 ANIMAL NUMBER: C91343 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:03 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 4 X 3 MM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE NEAR RIGHT HORN OF UTERUS: SINGLE MASS, 20 X 12 X 6 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (LUMBAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : -FIBROSIS,-SLIGHT, MULTI-FOCAL -NECROSIS, FAT,-PRESENT 3054 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1447 ANIMAL NUMBER: C91343 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:03 PROSECTOR: JODI THIEREN RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOt /fOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 3055 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1448 ANIMAL NUMBER: C91344 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 94; 60 X 50MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -CYST(S); ALONG MARGIN OF LEFT LATERAL LOBE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -CYST(S); RIGHT, NEAR CAUDAL POLE: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, TAN AND FIRM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -CYST,-MINIMAL, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : >UNREMARKABLE LN, MESENTERIC (MS) : >TISSUE MISSING OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT 3056 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1449 ANIMAL NUMBER: C91344 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 52 X 45 X 24 MM, TAN, LOBULATED, VASCULAR AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -BLOOD FILM TAKEN -DISCHARGE-NASAL; DRY RED MATERIAL -ANIMAL THIN VAGINA (VA) : >TISSUE MISSING CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3057 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1450 ANIMAL NUMBER: C91344 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 715 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 298.3 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 13:43 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 3058 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1451 ANIMAL NUMBER: C91345 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 185.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:58 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 7.8249 ------------------------------------------- ------------------------------- 4.2160 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3059 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1452 ANIMAL NUMBER: C91345 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 185.6 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 9:58 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 3060 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1453 ANIMAL NUMBER: C91346 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 9:54 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; CLEAR DISCHARGE; EYES; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 2 MM IN DIAMETER, RIGHT IS 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 8 MM, RED, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3061 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1454 ANIMAL NUMBER: C91346 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 9:54 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN FOCI, PINPOINT AND UP TO 1 MM IN DIAMETER GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW MOIST MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK RED AND BROWN CRUSTED MATERIAL STOMACH, GL (ST) : ^UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3062 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1455 ANIMAL NUMBER: C91346 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 06/11/99 STUDY DAY OF DEATH: 502 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: 272.8 GRAMS DATE AND TIME OF NECROPSY: 06/11/99 9:54 PROSECTOR: MICHELE MARGGI RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3063 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1456 ANIMAL NUMBER: C91347 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0292 .0302 2.1108 1.4951 1.5305 16.7366 1.5430 .0923 .1046 .3956 .0154 .0085 1.5954 .0100 % .0104 % .7261 % .5143 % .5265 % 5.7573 % .5308 % .0318 % .0360 % .1361 % .0053 % .0029 % .5488 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0138 .0143 1.0000 .7083 .7251 7.9290 .7310 .0437 .0496 .1874 .0073 .0040 .7558 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL LOBE: SINGLE TAN, SLIGHTLY RAISED FOCUS, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT, FOCAL 3064 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1457 ANIMAL NUMBER: C91347 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, PINPOINT AND UP TO 3 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3065 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1458 ANIMAL NUMBER: C91347 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 290.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:55 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3066 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1459 ANIMAL NUMBER: C91348 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 257.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0446 .0342 2.0387 1.1265 1.1467 17.0849 1.7072 .0846 .1174 .5881 .0100 .0073 .7012 .0173 % .0133 % .7920 % .4376 % .4455 % 6.6375 % .6632 % .0329 % .0456 % .2285 % .0039 % .0028 % .2724 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0219 .0168 1.0000 .5526 .5625 8.3803 .8374 .0415 .0576 .2885 .0049 .0036 .3439 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED LN, MESENTERIC (MS) -DIFFUSELY RED LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 3067 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1460 ANIMAL NUMBER: C91348 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 257.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES, DIFFUSE: MULTIPLE TAN AREAS, UP TO 5 X 2 MM PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE, PINPOINT FOCI GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : >UNREMARKABLE STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3068 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1461 ANIMAL NUMBER: C91348 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 257.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:23 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3069 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1462 ANIMAL NUMBER: C91349 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 410 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 15:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: BROWN LN, MESENTERIC (MS) : -MOTTLED; DIFFUSE: RED AND TAN LUNG (LU) : -UNCOLLAPSED; ALL LOBES GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DARK RED MOIST MATERIAL -MALOCCLUSION; BILATERAL: UPPER INCISORS -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND TAIL, DRY BROWN AND RED MATERIAL HISTOPATHOLOGY LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, DIFFUSE LN, MESENTERIC (MS) : >UNREMARKABLE THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : >UNREMARKABLE CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 3070 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1463 ANIMAL NUMBER: C91349 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/11/99 STUDY DAY OF DEATH: 410 STUDY WEEK OF DEATH: 59 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/11/99 15:09 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3071 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1464 ANIMAL NUMBER: C91350 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 178.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:37 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 6.4865 ------------------------------------------- ------------------------------- 3.6441 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3072 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1465 ANIMAL NUMBER: C91350 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 178.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 8:37 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 3073 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1466 ANIMAL NUMBER: C91351 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 9:58 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 84; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; LATERALTHORACIC-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); VISCERAL SURFACE OF LEFT LATERAL AND AT JUNCTION OF MEDIAN LOBES: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 3074 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1467 ANIMAL NUMBER: C91351 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 9:58 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 21 X 19 X 12 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE DARK RED, MOIST ULCERATED AREA, 13 X 11 X 5 MM; COLLECTED INTACT WITH MASS -THICKENED; ALL REGIONS, DIFFUSE: TAN; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID LN, MANDIBULAR (MN) : -DIFFUSELY RED; LEFT: DARK RED (CAP 23) LN, AXILLARY (AX) : -MOTTLED; RIGHT: RED AND TAN (CAP 22) -DIFFUSELY RED; LEFT: DARK RED (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL 3075 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1468 ANIMAL NUMBER: C91351 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 374.4 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 9:58 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3076 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1469 ANIMAL NUMBER: C91352 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 383.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:00 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0393 .0309 2.2330 1.6710 1.7530 24.5163 1.6006 .0683 .0809 .6160 .0146 .0174 1.3902 .0102 % .0081 % .5821 % .4356 % .4570 % 6.3911 % .4173 % .0178 % .0211 % .1606 % .0038 % .0045 % .3624 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0176 .0138 1.0000 .7483 .7850 10.9791 .7168 .0306 .0362 .2759 .0065 .0078 .6226 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -STASIS, BILE,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL 3077 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1470 ANIMAL NUMBER: C91352 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 383.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:00 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) -MASS(ES); 8 X 6 X 5 MM, DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3078 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1471 ANIMAL NUMBER: C91352 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 383.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:00 PROSECTOR: WENDY CHERRY RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3079 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1472 ANIMAL NUMBER: C91353 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 316.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:49 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0281 .0313 2.4047 1.4217 1.4900 17.4901 1.7296 .0924 .1090 .5484 .0170 .0262 .7222 .0089 % .0099 % .7600 % .4493 % .4709 % 5.5278 % .5466 % .0292 % .0345 % .1733 % .0054 % .0083 % .2283 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0117 .0130 1.0000 .5912 .6196 7.2733 .7193 .0384 .0453 .2281 .0071 .0109 .3003 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AREAS, UP TO 2 X 1 MM OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3080 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1473 ANIMAL NUMBER: C91353 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 316.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:49 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TAIL (TI) : -RAISED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE BROWN AREAS, UP TO 4 X 3 X 2 MM; SOME CONTAIN A GREEN, CASEOUS SEMISOLID; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL 3081 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1474 ANIMAL NUMBER: C91353 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 316.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:49 PROSECTOR: WENDY CHERRY RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3082 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1475 ANIMAL NUMBER: C91354 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 211.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 11:27 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; LIMBSFRONT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 5 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 8 X 8 X 4 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SEVERE, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, GL (ST) : -EDEMA,-MODERATE, DIFFUSE 3083 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1476 ANIMAL NUMBER: C91354 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 211.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 11:27 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN, WHITE ERODED AREA, 5 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; RED, DRY MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE AND FRONT LIMBS: YELLOW AND MOIST MATERIAL AND RED, DRY MATERIAL -ANIMAL THIN STOMACH, NONGL (SU) : -EDEMA,-MODERATE, DIFFUSE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE 3084 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1477 ANIMAL NUMBER: C91354 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/27/99 STUDY DAY OF DEATH: 610 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 211.9 GRAMS DATE AND TIME OF NECROPSY: 09/27/99 11:27 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE),ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT),VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE),ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3085 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1478 ANIMAL NUMBER: C91355 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 253.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:08 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0352 .0304 1.8820 1.1065 1.1411 10.1791 1.1450 .0538 .0520 .3082 .0109 .0082 .5800 .0139 % .0120 % .7439 % .4374 % .4510 % 4.0234 % .4526 % .0213 % .0206 % .1218 % .0043 % .0032 % .2292 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0187 .0162 1.0000 .5879 .6063 5.4087 .6084 .0286 .0276 .1638 .0058 .0044 .3082 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN OVARY (OV) : -HEMORRHAGE,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3086 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1479 ANIMAL NUMBER: C91355 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 253.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 13:08 PROSECTOR: DONIA MEYER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS) 3087 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1480 ANIMAL NUMBER: C91356 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 280.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:19 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTIONS KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN -CYST(S); BILATERAL, DIFFUSE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER OVARY (OV) : -CYST(S); LEFT: SINGLE GREEN FLUID FILLED CYST, 5 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL -PIGMENTED,-MODERATELY SEVERE, FOCAL -THROMBUS,-SEVERE, FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3088 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1481 ANIMAL NUMBER: C91356 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 280.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:19 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); ALL REGIONS, DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM -LARGE; 7 X 6 X 4 MM UTERUS (UT) : -CYST(S); WITHIN WALLS OF RIGHT HORN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATE, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3089 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1482 ANIMAL NUMBER: C91356 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 280.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:19 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3090 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1483 ANIMAL NUMBER: C91357 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 298.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:12 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 MM IN DIAMETER (RIGHT IS 4 X 3 X 3 MM) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGE,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT 3091 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1484 ANIMAL NUMBER: C91357 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 298.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:12 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 6 X 5 MM PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK RED, TAN AND SLIGHTLY RAISED AREA, 2 MM IN DIAMETER STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3092 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1485 ANIMAL NUMBER: C91357 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 298.0 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 8:12 PROSECTOR: JARED FISHEL RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3093 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1486 ANIMAL NUMBER: C91358 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 61; 50 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 70 X 35 X 30 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NOT IDENTIFIED HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL, MULTI FOCAL OVARY (OV) : -PIGMENTED,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -N-MAMMARY CARCINOMA,-NEOPLASM PRESENT PITUITARY (PI) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -NECROSIS,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 3094 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1487 ANIMAL NUMBER: C91358 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -MISSING/NOT IDENTIFIED; NOT IDENTIFIED DUE TO MASS GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL UTERUS (UT) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT LN, MANDIBULAR (MN) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION 3095 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1488 ANIMAL NUMBER: C91358 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 523 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 9:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL(CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3096 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1489 ANIMAL NUMBER: C91359 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/25/99 10:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: DARK BROWN AND TAN OVARY (OV) : -MISSING/NOT IDENTIFIED; LEFT: MISSING AT TIME OF NECROPSY HISTOPATHOLOGY LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -NECROSIS, INDIVIDUAL HEPATOCYTE,MINIMAL, MULTI-FOCAL -PIGMENT, KUPFFER CELL,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL OVARY (OV) : -ONE EXAMINED,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SEVERE, DIFFUSE 3097 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1490 ANIMAL NUMBER: C91359 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/25/99 10:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -LARGE, DIFFUSE; BILATERAL, DIFFUSE: EACH IS 74 X 20 X 20 MM -SEMIFLUID MATERIAL; BILATERAL, WITHIN LUMEN: LARGE AMOUNTS OF DARK BROWN MUCOID SEMIFLUID MATERIAL UTERUS (UT) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT 3098 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1491 ANIMAL NUMBER: C91359 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 546 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/25/99 10:51 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD), GENERAL COMMENT (GC) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3099 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1492 ANIMAL NUMBER: C91360 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:32 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) -MOTTLED; ALL LOBES: RED AND TAN OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR FLUID FILLED CYSTS ON EACH, UP TO 2 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGE,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT 3100 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1493 ANIMAL NUMBER: C91360 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:32 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; DRY RED MATERIAL -BONE MARROW SMEAR TAKEN ENTIRE HEAD: PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3101 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1494 ANIMAL NUMBER: C91360 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 328.8 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 7:32 PROSECTOR: TONY HANFELD RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3102 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1495 ANIMAL NUMBER: C91361 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 338.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MASS(ES); LEFT LATERAL LOBE: SINGLE MASS, 15 MM IN DIAMETER, RED, BROWN, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NOT POSITIVELY IDENTIFIED (CAP 20) OVARY (OV) : -CYST(S); BILATERAL: LEFT HAS A SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER, RIGHT HAS A SINGLE RED FLUID FILLED CYST, 7 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SEVERE, FOCAL -M-CARCINOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL OVARY (OV) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -PIGMENTED,-MODERATE, MULTI-FOCAL -THROMBUS,-SEVERE, FOCAL -B-GRANULOSA/THECA CELL TUMOR,-NEOPLASM PRESENT 3103 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1496 ANIMAL NUMBER: C91361 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 338.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : >TISSUE MISSING >NOTE:>HEPATIC LYMPH NODE NOT PRESENT ON SECTION 3104 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1497 ANIMAL NUMBER: C91361 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 338.6 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 8:07 PROSECTOR: TERRI L. WILLIAMS RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3105 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1498 ANIMAL NUMBER: C91362 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:27 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 85; 90 X 65MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 89; 50 X 40MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT JUNCTION OF RIGHT AND LEFT MEDIAN LOBES: SINGLE TAN AREA, 4 X 3 MM; COLLECTED ON ROUTINE SECTION -MOTTLED; ALL LOBES: RED, TAN AND BROWN -MASS(ES); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE MASS, 14 X 14 X 3 MM, RED AND FIRM; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NOT IDENTIFIED PITUITARY (PI) : -LARGE; 5 X 5 X 3 MM; APPEARS SWOLLEN HISTOPATHOLOGY LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL OVARY (OV) : -MINERALIZATION,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 3106 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1499 ANIMAL NUMBER: C91362 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:27 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 40 X 35 X 14 MM, RED, TAN, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 90 X 70 X 55 MM (185.3 GRAMS) RED, DARK RED, TAN, FIRM, VASCULAR AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 3107 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1500 ANIMAL NUMBER: C91362 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 644.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 8:27 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3108 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1501 ANIMAL NUMBER: C91363 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 295.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:35 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >HOUSING,FOOD,WATER & MAINT.; TEETH CUT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN SPLEEN (SP) : -LIGHT FOCUS(I)/AREA(S); NEAR EACH POLE: MULTIPLE GREY AREAS, UP TO 12 X 5 MM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 3 X 2 MM VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL SPLEEN (SP) : >UNREMARKABLE LN, MESENTERIC (MS) : -DILATATION, SINUSOIDAL,-MINIMAL, MULTI FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL VAGINA (VA) : >UNREMARKABLE 3109 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1502 ANIMAL NUMBER: C91363 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 295.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:35 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; EXTENDING INTO ENTIRE ABDOMINAL REGION: DARK YELLOW AND BROWN -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 3110 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1503 ANIMAL NUMBER: C91363 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 295.2 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 14:35 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 3111 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1504 ANIMAL NUMBER: C91364 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 9:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); DORSAL SURFACE OF LEFT LOBE AND RIGHT CAUDAL LOBE: MULTIPLE WHITE AREAS, UP TO 12 X 7 MM PITUITARY (PI) : -MASS(ES); 14 X 10 X 10 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SKIN (SK) : -ATROPHY, ADNEXAL,-MODERATE, MULTI FOCAL 3112 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1505 ANIMAL NUMBER: C91364 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 9:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -ALOPECIA-FOCAL; RIGHT FLANK: SINGLE AREA, 35 X 18 MM; AREA ALSO HAS MULTIPLE TAN AND DRY CRUSTED AREAS, UP TO 3 X 2 MM; COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK YELLOW AND MOIST -DISCHARGE-NASAL; DARK RED AND DRY -ANIMAL THIN SKIN (SK) : -INFLAMMATION, CHRONIC,-MODERATELY SEVERE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3113 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1506 ANIMAL NUMBER: C91364 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/02/00 9:23 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3114 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1507 ANIMAL NUMBER: C91365 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 240.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:59 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0314 .0325 2.0802 1.1270 1.1918 10.9570 1.4156 .0533 .0604 .4329 .0257 .0242 1.2583 .0131 % .0135 % .8653 % .4688 % .4958 % 4.5578 % .5889 % .0222 % .0251 % .1801 % .0107 % .0101 % .5234 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0151 .0156 1.0000 .5418 .5729 5.2673 .6805 .0256 .0290 .2081 .0124 .0116 .6049 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE BROWN FOCUS, 1 MM IN DIAMETER UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, GL (ST) : ^UNREMARKABLE UTERUS (UT) : -DILATATION,-MINIMAL, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3115 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1508 ANIMAL NUMBER: C91365 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 240.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 13:59 PROSECTOR: TONY HANFELD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3116 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1509 ANIMAL NUMBER: C91366 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:27 PROSECTOR: MICHELE MARGGI RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 74; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 80; 35 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PARATHYROID (PT) : -LARGE; LEFT: 4 MM IN DIAMETER (RIGHT IS 1 MM IN DIAMETER) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 10 X 7 MM, DARK RED, TAN, GREY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL OVARY (OV) : -HEMORRHAGE,-MINIMAL, FOCAL LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3117 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1510 ANIMAL NUMBER: C91366 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:27 PROSECTOR: MICHELE MARGGI RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 35 X 25 X 15 MM, RED, DARK RED, TAN, VASCULAR, AND SOFT; ON SECTION EXUDES A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 21) -MASS #2; LEFT PERINEUM REGION, EXTENDING TO ANUS: SINGLE MASS, 40 X 30 X 20 MM, TAN, GREY, BROWN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 22); POSSIBLY CONSISTENT WITH MASS (VENTRALABDOMINAL-LEFT) AS NOTED IN CLINICAL OBSERVATIONS UTERUS (UT) : -CYST(S); LEFT, WITHIN LUMEN: SINGLE CLEAR FLUID FILLED CYST, 4 X 2 X 2 MM TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE DARK BROWN CRUSTED AREAS, UP TO 2 X 1 MM; SOME AREAS EXUDE A LIGHT GREEN CASEOUS MATERIAL; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT UTERUS (UT) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL LN, INGUINAL (IN) : >TISSUE MISSING 3118 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1511 ANIMAL NUMBER: C91366 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 319.5 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 14:27 PROSECTOR: MICHELE MARGGI RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 3119 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1512 ANIMAL NUMBER: C91367 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 307.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0398 .0388 2.1356 1.7518 1.8977 17.4093 1.7508 .0740 .0761 .5978 .0128 .0122 1.3167 .0129 % .0126 % .6938 % .5691 % .6165 % 5.6560 % .5688 % .0240 % .0247 % .1942 % .0042 % .0040 % .4278 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0186 .0182 1.0000 .8203 .8886 8.1519 .8198 .0347 .0356 .2799 .0060 .0057 .6165 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, MULTI-FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT UTERUS (UT) : >UNREMARKABLE 3120 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1513 ANIMAL NUMBER: C91367 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 307.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BROKEN, UPPER LEFT INCISOR CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3121 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1514 ANIMAL NUMBER: C91367 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 307.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:16 PROSECTOR: JODI THIEREN RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3122 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1515 ANIMAL NUMBER: C91368 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 270.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:14 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0410 .0431 2.1117 1.2641 1.4674 19.0978 1.4081 .0759 .0568 .4751 .0120 .0160 1.0793 .0152 % .0159 % .7804 % .4671 % .5423 % 7.0576 % .5204 % .0280 % .0210 % .1756 % .0044 % .0059 % .3989 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0194 .0204 1.0000 .5986 .6949 9.0438 .6668 .0359 .0269 .2250 .0057 .0076 .5111 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3123 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1516 ANIMAL NUMBER: C91368 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 270.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 14:14 PROSECTOR: GREGORY CODDINGTON RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM),MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS(ES), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE),ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3124 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1517 ANIMAL NUMBER: C91369 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 448.4 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:00 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 45; 55 X 55MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 63; 20 X 25MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 67; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED -DIFFUSELY DARK; ALL LOBES: DARK BROWN SPLEEN (SP) : -LARGE; 60 X 13 X 8 MM PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3125 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1518 ANIMAL NUMBER: C91369 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 448.4 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:00 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 63 X 57 X 45 MM, RED, DARK RED, TAN, BROWN, FIRM AND LOBULATED; SAME ON CUT SURFACE; OVERLYING SKIN HAS MULTIPLE DRY RED AND BROWN CRUSTED AREAS, UP TO 45 X 25 MM (CAP 20); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #2; RIGHT AXILLARY REGION, EXTENDS TO VENTRAL THORACIC REGION: SINGLE MASS, 37 X 23 X 12, RED, TAN, BROWN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #3; LEFT AXILLARY REGION, CAUDAL TO MASS #2: SINGLE MASS, 20 X 14 X 8 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (AXILLARY) NORMAL (CAP 21) -MASS #4; LEFT INGUINAL REGION: SINGLE MASS, 33 X 21 X 14 MM, RED, DARK RED, TAN, WHITE, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED SKIN (SK) : -ULCERATION,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -NECROSIS,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,- 4 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT 3126 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1519 ANIMAL NUMBER: C91369 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 448.4 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:00 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3127 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1520 ANIMAL NUMBER: C91370 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 209.2 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 9:25 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0375 .0361 1.7300 .7688 .7898 9.3806 .9689 .0502 .0563 .4589 .0071 .0066 .5249 .0179 % .0173 % .8270 % .3675 % .3775 % 4.4840 % .4631 % .0240 % .0269 % .2194 % .0034 % .0032 % .2509 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0217 .0209 1.0000 .4444 .4565 5.4223 .5601 .0290 .0325 .2653 .0041 .0038 .3034 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3128 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1521 ANIMAL NUMBER: C91370 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 209.2 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 9:25 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3129 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1522 ANIMAL NUMBER: C91371 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 380.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:01 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0370 .0357 2.2665 1.8602 1.8759 17.1837 1.7414 .0631 .0662 .6717 .0236 .0190 1.4559 .0097 % .0094 % .5964 % .4895 % .4937 % 4.5220 % .4583 % .0166 % .0174 % .1768 % .0062 % .0050 % .3831 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0163 .0158 1.0000 .8207 .8277 7.5816 .7683 .0278 .0292 .2964 .0104 .0084 .6424 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >MASS(ES); AXILLARY REGION-RIGHT; WEEK 52; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 3130 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1523 ANIMAL NUMBER: C91371 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 380.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:01 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND WHITE CREAMY SEMIFLUID FILLED CYSTS, TO 7 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION; POSSIBLY CONSISTENT WITH MASS (RIGHT AXILLARY REGION) NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION 3131 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1524 ANIMAL NUMBER: C91371 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, FOURTH INTERIM SACRIFICE DATE OF DEATH: 01/27/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 380.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/99 11:01 PROSECTOR: KIMBERLY DURLAND RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: MATTHEW AXTMAN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3132 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1525 ANIMAL NUMBER: C91372 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 358.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE AREAS, UP TO 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY EYE (EY) : -HYPERPLASIA, CORNEAL EPITHELIUM,SLIGHT, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -THROMBUS,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3133 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1526 ANIMAL NUMBER: C91372 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 358.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 8 X 4 MM, RED, DARK RED, GRAY, TAN, AND FIRM UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3134 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1527 ANIMAL NUMBER: C91372 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/26/00 STUDY DAY OF DEATH: 731 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 358.3 GRAMS DATE AND TIME OF NECROPSY: 01/26/00 10:16 PROSECTOR: JODI THIEREN RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3135 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1528 ANIMAL NUMBER: C91373 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 16:03 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES: THICKENED -MOTTLED; ALL LOBES: DARK BROWN AND TAN KIDNEY (KD) : -MOTTLED; BILATERAL: BROWN AND TAN OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH; EACH IS 8 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 13 X 8 X 8 MM, RED, DARK RED, GRAY AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 4 X 2 MM HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL OVARY (OV) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL 3136 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1529 ANIMAL NUMBER: C91373 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 16:03 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 21) VAGINA (VA) : -SEMIFLUID MATERIAL; LIGHT GREEN AND MUCOID LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED AND TAN (CAP 22) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOWISH, BROWN AND MOIST -BLOOD FILM TAKEN -DISCHARGE-NASAL; RED AND DRY -STAINS - SKIN/HAIRCOAT; ABDOMEN, CHEST AND HEAD: DARK RED AND DRY -ANIMAL THIN STOMACH, NONGL (SU) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT VAGINA (VA) : ^UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE 3137 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1530 ANIMAL NUMBER: C91373 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 256.2 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 16:03 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 3138 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1531 ANIMAL NUMBER: C91374 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 235.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:23 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); TAIL-PROXIMAL; WEEK 88; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); BILATERAL: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, TAN, RED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -VACUOLATION,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3139 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1532 ANIMAL NUMBER: C91374 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 235.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:23 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE TAIL: MULTIPLE DRY DARK BROWN AND CRUSTED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK BROWN MATERIAL -DISCHARGE-NASAL; DRY DARK BROWN MATERIAL -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: MOIST YELLOW MATERIAL HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, GL (ST) : >UNREMARKABLE COLON (CO) : -EDEMA,-SLIGHT, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL 3140 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1533 ANIMAL NUMBER: C91374 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 235.7 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 13:23 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SM), 3141 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1534 ANIMAL NUMBER: C91375 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 272.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, DARK RED, TAN, LOBULATED AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3142 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1535 ANIMAL NUMBER: C91375 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 272.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -DARK FOCUS(I)/AREA(S); MUCOSA, NEAR JUNCTION OF GLANDULAR MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY DARK RED MATERIAL STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, DIFFUSE -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -ULCERATION,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3143 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1536 ANIMAL NUMBER: C91375 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/27/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 272.3 GRAMS DATE AND TIME OF NECROPSY: 08/27/99 8:02 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3144 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1537 ANIMAL NUMBER: C91376 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 100; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 105; THREE MASSES UNDER 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DARK FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK BROWN AREAS, UP TO 5 MM IN DIAMETER; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTION OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 15 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED, GRAY AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -CYST,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL OVARY (OV) : -CYST,-PRESENT BONE, STERNUM (SB) : -HYPERPLASIA, CARTILAGE,-MODERATE, FOCAL THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 3145 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1538 ANIMAL NUMBER: C91376 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 38 X 27 X 20 MM, RED, DARK RED, BROWN, TAN, FIRM, LOBULATED AND VASCULAR; ON SECTION, SOME AREAS EXUDE A BROWN FLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 30 X 10 MM, RED, TAN, DARK BROWN, SOFT AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -LARGE, FOCAL; BILATERAL, DISTAL PORTION OF EACH HORN: UP TO 12 MM IN DIAMETER LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -FLUID-ABDOMINAL CAVITY; LARGE AMOUNTS OF LIGHT BROWN FLUID MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT UTERUS (UT) : -CYST, KERATIN,-PRESENT -DILATATION,-SEVERE, DIFFUSE -HYPERPLASIA, ENDOMETRIAL,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -INFILTRATE, MACROPHAGE, PIGMENTED,MODERATE, MULTI-FOCAL 3146 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1539 ANIMAL NUMBER: C91376 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.2 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 7:42 PROSECTOR: JODI THIEREN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3147 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1540 ANIMAL NUMBER: C91377 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/06/99 STUDY DAY OF DEATH: 497 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/06/99 13:17 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-RIGHT; WEEK 43; 90 X 85MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY HISTOPATHOLOGY LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL JEJUNUM (JE) : >AUTOLYTIC SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, FOCAL 3148 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1541 ANIMAL NUMBER: C91377 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/06/99 STUDY DAY OF DEATH: 497 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/06/99 13:17 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 110 X 80 X 60 MM, RED, DARK RED, TAN, FIRM, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20); OVERLYING SKIN HAS A SINGLE DARK RED AND BROWN CRUSTED AREA, 70 X 60 MM; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -DISCHARGE-NASAL; BILATERAL: CLEAR MAMMARY, FEMALE (MF) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 3149 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1542 ANIMAL NUMBER: C91377 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/06/99 STUDY DAY OF DEATH: 497 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/06/99 13:17 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3150 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1543 ANIMAL NUMBER: C91378 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -CYST(S); LEFT, CORTICAL SURFACE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, EACH IS 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MINIMAL, MULTI-FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CYST,-MODERATELY SEVERE, FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -M-THYMIC CARCINOMA,-NEOPLASM PRESENT 3151 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1544 ANIMAL NUMBER: C91378 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 7 MM, RED, DARK RED, TAN, AND SOFT UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: TAN FLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3152 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1545 ANIMAL NUMBER: C91378 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 7:38 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3153 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1546 ANIMAL NUMBER: C91379 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:00 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN S T A rr U S LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0324 .0285 1.7676 .8778 .9029 8.1754 1.0785 .0520 .0522 .3743 .0076 .0087 .5700 .0141 % .0124 % .7689 % .3818 % .3927 % 3.5561 % .4691 % .0226 % .0227 % .1628 % .0033 % .0038 % .2479 % .0183 .0161 1.0000 .4966 .5108 4.6251 .6101 .0294 .0295 .2118 .0043 .0049 .3225 WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE LINEAR AREAS, UP TO 7 X 1 MM GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CYST,-SLIGHT, FOCAL STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3154 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1547 ANIMAL NUMBER: C91379 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/29/98 STUDY DAY OF DEATH: 94 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.9 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 14:00 PROSECTOR: TODD ACKER RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JERALD W. JOHNSON THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3155 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1548 ANIMAL NUMBER: C91380 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 92; 35 X 30MM; VERIFIED AT NECROPSY: OBSERVED >EYES; DISCOLORED/LIGHT RED; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 100; 35 X 35MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : -RED INTRAOCULAR MATERIAL; RIGHT BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LARGE; ALL LOBES: THICKENED -DIFFUSELY DARK; ALL LOBES: DARK BROWN ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE LEFT ADRENAL: SINGLE MASS, 15 X 10 X 10 MM, RED, DARK RED, TAN, BROWN AND SOFT; SAME ON CUT SURFACE; REGIONAL LN (SEE RENAL) HISTOPATHOLOGY EYE (EY) : -HEMORRHAGE,-MODERATE, FOCAL -INFLAMMATION, ACUTE, CILIARY,-MINIMAL MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SEVERE, MULTI-FOCAL -ATROPHY, CORTICAL,-MODERATELY SEVERE, DIFFUSE -HEMORRHAGE,-SEVERE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL 3156 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1549 ANIMAL NUMBER: C91380 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 5 X 5 MM, RED, DARK RED, GRAY, TAN AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 55 X 45 X 30 MM, RED, DARK RED, TAN, FIRM AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 25 X 15 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DARK RED AND CRUSTED AREA, 10 X 5 MM; COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS, DIFFUSE (CAP 20) -MASS #3; RIGHT PERINEAL REGION: SINGLE MASS, 60 X 40 X 20 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : -HYPERPLASIA, GLANDULAR,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT 3157 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1550 ANIMAL NUMBER: C91380 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -THICKENED WALL; BILATERAL, DIFFUSE -MASS(ES); SEROSAL SURFACE OF LEFT DISTAL HORN: SINGLE MASS, 18 X 15 X 10 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 22) LN, MEDIASTINAL (ML) : -LARGE; UP TO 5 MM IN DIAMETER (CAP 21) LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 23) LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS UP TO 12 X 8 X 3 MM -DIFFUSELY RED; BILATERAL: DARK RED (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN UTERUS (UT) : -M-LEIOMYOSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, RENAL (RL) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, AXILLARY (AX) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3158 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1551 ANIMAL NUMBER: C91380 SEX: FEMALE DOSE GROUP: 4 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 01/25/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 401.9 GRAMS DATE AND TIME OF NECROPSY: 01/25/00 9:35 PROSECTOR: NANCY DIEDRICH RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 3159 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1552 ANIMAL NUMBER: C91393 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 146.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:43 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0267 .0249 1.6759 .7304 .7355 10.9693 .7636 .0443 .0472 .3482 .0005 .0092 .3120 .0182 % .0170 % 1.1408 % .4972 % .5007 % 7.4672 % .5198 % .0302 % .0321 % .2370 % .0003 % .0063 % .2124 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0159 .0149 1.0000 .4358 .4389 6.5453 .4556 .0264 .0282 .2078 .0003 .0055 .1862 ORGAN S T A T1 U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN EXCLUDE WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3160 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1553 ANIMAL NUMBER: C91393 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 146.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:43 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3161 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1554 ANIMAL NUMBER: C91394 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 184.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:10 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0331 .0298 1.8957 .7737 .8100 12.7034 .8192 .0444 .0462 .3206 .0051 .0054 .4876 .0180 % .0162 % 1.0286 % .4198 % .4395 % 6.8928 % .4445 % .0241 % .0251 % .1740 % .0028 % .0029 % .2646 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0175 .0157 1.0000 .4081 .4273 6.7012 .4321 .0234 .0244 .1691 .0027 .0028 .2572 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED THYMUS (TH) : -RED FOCUS(I)/AREA(S); RIGHT LOBE: MULTIPLE RED PINPOINT FOCI STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 10 X 1 MM LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE THYMUS (TH) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL STOMACH, GL (ST) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3162 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1555 ANIMAL NUMBER: C91394 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 184.3 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 8:10 PROSECTOR: WENDY CHERRY RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL NECROPSY OUT OF ORDER ANIMAL DIED PRIOR TO EXSANGUINATION THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3163 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1556 ANIMAL NUMBER: C91404 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 175.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:52 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0344 .0385 1.7374 .8122 .7923 12.2573 .8984 .0571 .0647 .3259 .0099 .0057 .5221 .0196 % .0219 % .9877 % .4617 % .4504 % 6.9683 % .5107 % .0325 % .0368 % .1853 % .0056 % .0032 % .2968 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0198 .0222 1.0000 .4675 .4560 7.0550 .5171 .0329 .0372 .1876 .0057 .0033 .3005 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -LARGE; ALL LOBES GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATELY SEVERE, DIFFUSE -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3164 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1557 ANIMAL NUMBER: C91404 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 175.9 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:52 PROSECTOR: TODD ACKER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3165 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1558 ANIMAL NUMBER: C91415 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 174.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:19 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0249 .0175 1.8553 .7904 .7924 11.1073 .8856 .0367 .0467 .3928 .0065 .0110 .3142 .0143 % .0100 % 1.0620 % .4524 % .4536 % 6.3579 % .5069 % .0210 % .0267 % .2248 % .0037 % .0063 % .1799 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0134 .0094 1.0000 .4260 .4271 5.9868 .4773 .0198 .0252 .2117 .0035 .0059 .1694 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: DARK BROWN GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE 3166 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1559 ANIMAL NUMBER: C91415 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 174.7 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:19 PROSECTOR: GREGORY CODDINGTON RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3167 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1560 ANIMAL NUMBER: C91423 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:52 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 8.2679 ------------------------------------------- ------------------------------- 4.4213 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3168 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1561 ANIMAL NUMBER: C91423 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.0 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:52 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 3169 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1562 ANIMAL NUMBER: C91427 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 198.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 8.2478 ------------------------------------------- ------------------------------- 4.1593 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3170 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1563 ANIMAL NUMBER: C91427 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 198.3 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:30 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 3171 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1564 ANIMAL NUMBER: C91430 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 161.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:06 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 8.6128 ------------------------------------------- ------------------------------- 5.3264 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3172 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1565 ANIMAL NUMBER: C91430 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 161.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:06 PROSECTOR: DONIA MEYER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 3173 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1566 ANIMAL NUMBER: C91438 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 169.8 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:30 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) .0329 .0308 1.9238 .8302 .8670 11.6597 .9038 .0514 .0589 .4120 .0087 .0076 .7756 .0194 % .0181 % 1.1330 % .4889 % .5106 % 6.8667 % .5323 % .0303 % .0347 % .2426 % .0051 % .0045 % .4568 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0171 .0160 1.0000 .4315 .4507 6.0608 .4698 .0267 .0306 .2142 .0045 .0040 .4032 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -LARGE; ALL LOBES -MOTTLED; ALL LOBES: DARK BROWN AND TAN -DIFFUSELY DARK; ALL LOBES: DARK BROWN UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: CLEAR FLUID GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -HYPERTROPHY, CENTRILOBULAR, HEPATOCELLULAR,-MODERATE, DIFFUSE UTERUS (UT) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3174 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1567 ANIMAL NUMBER: C91438 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 03/20/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: 169.8 GRAMS DATE AND TIME OF NECROPSY: 03/20/98 10:30 PROSECTOR: DONIA MEYER RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TERRI L. WILLIAMS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV) 3175 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1568 ANIMAL NUMBER: C91443 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 190.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 11.7999 ------------------------------------------- ------------------------------- 6.1877 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3176 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1569 ANIMAL NUMBER: C91443 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 190.7 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:30 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 3177 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1570 ANIMAL NUMBER: C91448 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 170.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:36 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LEFT THYROID/PAR (TT0) RIGHT THYROID/PR (TT1) UTERUS (UT) ------------------------------- 9.2407 ------------------------------------------- ------------------------------- 5.4293 % ------------------------------------------- ------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3178 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1571 ANIMAL NUMBER: C91448 SEX: FEMALE DOSE GROUP: 5 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 02/18/98 STUDY DAY OF DEATH: 24 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 170.2 GRAMS DATE AND TIME OF NECROPSY: 02/18/98 11:36 PROSECTOR: TODD ACKER RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: JILL PAUS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) 3179 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1572 ANIMAL NUMBER: C91451 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 92; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 98; 15 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE TAN AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT LATERAL LOBE: SINGLE MASS, 15 X 10 X 7 MM, TAN, RED, BROWN AND SOFT; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (HEPATIC) NOT IDENTIFIED LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 10 X 7 MM PITUITARY (PI) : -MASS(ES); 10 X 9 X 6 MM, RED, DARK RED, TAN, GRAY AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 X 2 MM HISTOPATHOLOGY BRAIN (BR) : >UNREMARKABLE LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, MULTI FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 3180 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1573 ANIMAL NUMBER: C91451 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 25 MM IN DIAMETER, TAN, RED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #2; MID INGUINAL REGION: SINGLE MASS, 35 X 20 X 15 MM, RED, DARK RED, TAN AND LOBULATED; ON SECTION EXUDES A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION LN, AXILLARY (AX) : -MOTTLED; BILATERAL: RED AND TAN (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -M-CARCINOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : >UNREMARKABLE 3181 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1574 ANIMAL NUMBER: C91451 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), PANCREAS (PA), URINARY BLADDER (UB) 3182 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1575 ANIMAL NUMBER: C91452 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 564.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 43; 80 X 70MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LUNG (LU) : -MASS(ES); RIGHT MIDDLE LOBE: SINGLE MASS, 27 X 16 X 10 MM, RED, DARK RED, TAN, FIRM AND SMOOTH; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 21) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 4 X 4 MM, RED, DARK RED, TAN, SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL LUNG (LU) : -EDEMA, ALVEOLAR,-MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, FOCAL -N-MAMMARY CARCINOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -EDEMA,-MODERATE, MULTI-FOCAL -GALACTOCELE,-SEVERE, MULTI-FOCAL 3183 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1576 ANIMAL NUMBER: C91452 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 564.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT CERVICAL REGION: SINGLE MASS, 95 X 72 X 41 MM (121.4 GRAMS), SOME AREAS ARE SOFT AND SOME ARE FIRM, RED, DARK RED, TAN AND BROWN; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDE A DARK BROWN FLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT CERVIX (CV) : >TISSUE MISSING CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT 3184 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1577 ANIMAL NUMBER: C91452 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 564.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), HEMATO NEOPLASIA (HN) 3185 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1578 ANIMAL NUMBER: C91453 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 315.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED, BROWN, AND TAN KIDNEY (KD) : -MASS(ES); BILATERAL, CORTICAL SURFACE, EXTENDING INTO MEDULLA: SINGLE MASS ON EACH; LEFT IS 20 X 15 X 8 MM, RIGHT IS 15 X 13 X 5 MM; BOTH ARE RED, TAN, SOFT, AND LOBULATED; ON SECTION OF EACH MASS, SOME AREAS EXUDED A GREEN SEMIFLUID; BOTH MASSES COLLECTED INTACT; REGIONAL LN (SEE RENAL) ADRENAL, CORTEX (AC) : -MASS(ES); RIGHT, INVOLVING ENTIRE ORGAN: 20 MM IN DIAMETER, RED, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE RENAL) -SMALL; LEFT: 4 X 2 X 2 MM HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -BACTERIAL COLONIES,-PRESENT -PYELONEPHRITIS,-MODERATELY SEVERE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ATROPHY, CORTICAL,-MODERATELY SEVERE, DIFFUSE -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MODERATELY SEVERE, MULTI-FOCAL -NECROSIS, CORTICAL,-MODERATELY SEVERE MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT 3186 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1579 ANIMAL NUMBER: C91453 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 315.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MESENTERIC (MS) : -GELATINOUS; DIFFUSE: CLEAR OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE RED AREA, 1 MM IN DIAMETER LN, MESENTERIC (MS) : >TISSUE MISSING OVARY (OV) : -CYST,-PRESENT LUNG (LU) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL, FOCAL -MINERALIZATION,-MODERATELY SEVERE, MULTI-FOCAL -NECROSIS,-MODERATELY SEVERE, MULTI FOCAL -N-ADRENAL CORTICAL CARCINOMA,-NEOPLASM PRESENT THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL 3187 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1580 ANIMAL NUMBER: C91453 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 315.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT CAVITY, ABDOM (PC) : CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE, -NECROSIS, FAT,-PRESENT NEAR RIGHT UTERINE HORN: SINGLE MASS, 5 X 4 X 2 MM, YELLOW AND FIRM (CAP 21) ; REGIONAL LN (LUMBAR) NORMAL (CAP 22) LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, RENAL (RL) : LN, RENAL (RL) : -DIFFUSELY RED; BILATERAL: (CAP 20) -HEMORRHAGE,-MODERATE, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HIND FEET: -EROSION,-MODERATELY SEVERE, MULTI SINGLE TAN CRUSTED AREA ON EACH, LEFT FOCAL IS 4 MM IN DIAMETER, RIGHT IS 9 MM IN -FIBROSIS,-MODERATE, MULTI-FOCAL DIAMETER, BOTH COLLECTED -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL THIN -BONE MARROW SMEAR TAKEN 3188 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1581 ANIMAL NUMBER: C91453 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 315.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) 3189 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1582 ANIMAL NUMBER: C91454 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 220.1 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 10:16 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADCRANIAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; DORSALCERVICAL; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DARK FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE DARK BROWN AREA, 2 MM IN DIAMETER LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); RIGHT CAUDAL AND LEFT LOBES, NEAR MARGIN: MULTIPLE TAN AREAS, UP TO 3 X 2 MM STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA NEAR JUNCTION OF GLANDULAR MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 1 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -HEMORRHAGE,-MODERATE, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EROSION,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -UNDETERMINED,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL 3190 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1583 ANIMAL NUMBER: C91454 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 220.1 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 10:16 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD REGION, DIFFUSE: DARK RED AND DRY MATERIAL -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: UP TO 15 MM IN DIAMETER; FILLED WITH GAS LN, MEDIASTINAL (ML) : >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SECTION 3191 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1584 ANIMAL NUMBER: C91454 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 220.1 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 10:16 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3192 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1585 ANIMAL NUMBER: C91455 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREA(S); RIGHT, WITHIN CORTEX: SINGLE DARK RED, RAISED AREA, 3 MM IN DIAMETER OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 10 MM IN DIAMETER, RIGHT IS 7 MM IN DIAMETER PITUITARY (PI) : -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA, NEAR PYLORIC SPHINCTER: TWO TAN AND RAISED AREAS, EACH IS 1 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -THROMBUS,-SLIGHT, FOCAL OVARY (OV) : -CYST,-PRESENT -ONE EXAMINED,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL, MULTI-FOCAL 3193 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1586 ANIMAL NUMBER: C91455 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR AND RED FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 3194 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1587 ANIMAL NUMBER: C91455 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), ADRENAL, MEDULLA (MA), LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 3195 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1588 ANIMAL NUMBER: C91456 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 342.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); RIGHT: MULTIPLE DARK BROWN AREAS, UP TO 3 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 13 X 9 X 8 MM, RED, DARK RED, TAN AND FIRM STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN FOCUS, 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE WHITE CREAMY SEMIFLUID FILLED CYSTS, 5 X 3 X 2 MM (CAP 20) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL 3196 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1589 ANIMAL NUMBER: C91456 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 342.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3197 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1590 ANIMAL NUMBER: C91456 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 533 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 342.7 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:10 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3198 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1591 ANIMAL NUMBER: C91457 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 513.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 84; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAL LOBE: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 X 5 X 5 MM (LEFT IS 5 X 4 X 3 MM) PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MODERATE, MULTI-FOCAL -THROMBUS,-MODERATE, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT 3199 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1592 ANIMAL NUMBER: C91457 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH:: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 513.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT PERINEAL REGION: SINGLE MASS, 10 MM IN DIAMETER, DARK RED, TAN, BROWN AND SOFT; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); WITHIN LUMEN OF MID RIGHT HORN: SINGLE CLEAR FLUID FILLED CYST, 12 X 6 X 6 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; RED AND MOIST UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3200 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1593 ANIMAL NUMBER: C91457 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/23/99 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 513.2 GRAMS DATE AND TIME OF NECROPSY: 11/23/99 13:46 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3201 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1594 ANIMAL NUMBER: C91458 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/05/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 340.2 GRAMS DATE AND TIME OF NECROPSY: 04/05/99 14:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 62; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; PROLAPSED; VAGINA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); MID ASPECT: SINGLE BLACK AREA, 3 X 1 MM -LARGE; 5 X 4 X 3 MM; APPEARS SWOLLEN HISTOPATHOLOGY SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -HEMORRHAGE,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CERVIX (CV) : -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE >NOTE:>AN ENDOMETRIAL STROMAL POLYP WAS PRESENT IN THE CERVIX 3202 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1595 ANIMAL NUMBER: C91458 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/05/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 340.2 GRAMS DATE AND TIME OF NECROPSY: 04/05/99 14:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY VAGINA (VA) : -MASS(ES); WITHIN LUMEN, EXTENDING FROM CERVIX THROUGH VULVA: SINGLE MASS, 38 X 28 X 20 MM, RED, DARK RED, GRAY, TAN, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 20); POSSIBLY CONSISTENT WITH PROLAPSE (VAGINA) AS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN CAUSE OF DEATH (CD) : -ENDOMETRIAL STROMAL POLYP,-PRESENT 3203 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1596 ANIMAL NUMBER: C91458 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 04/05/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 340.2 GRAMS DATE AND TIME OF NECROPSY: 04/05/99 14:36 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN), LN, LUMBAR/ILIAC (LM) 3204 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1597 ANIMAL NUMBER: C91459 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 550.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 102; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE, NEAR JUNCTION WITH LEFT MEDIAN LOBE: SINGLE TAN AREA, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE DARK RED AREA, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 14 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATE, FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, FOLLICULAR CELL,MODERATE, FOCAL 3205 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1598 ANIMAL NUMBER: C91459 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 550.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 6 X 3 MM, RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -THICKENED WALL; NEAR JUNCTION WITH NONGLANDULAR STOMACH MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 30 X 25 X 15 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP T0 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -MASS #2; LEFT AXILLARY REGION: TWO MASSES, EACH ARE 10 MM IN DIAMETER, BOTH ARE TAN AND FIRM; SAME ON CUT SURFACES; BOTH COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 20) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION CERVIX (CV) : -MASS(ES); ATTACHED TO VENTRAL SEROSAL SURFACE: SINGLE MASS, 6 MM IN DIAMETER, TAN AND FIRM; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT CERVIX (CV) : -M-LEIOMYOSARCOMA,-NEOPLASM PRESENT 3206 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1599 ANIMAL NUMBER: C91459 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 550.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY VAGINA (VA) : -MASS(ES); SEROSAL SURFACE, NEAR BLADDER: SINGLE MASS, 7 X 5 X 3 MM, TAN, FIRM AND LOBULATED; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN VAGINA (VA) : -I-LEIOMYOSARCOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3207 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1600 ANIMAL NUMBER: C91459 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 550.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:09 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM), LN, AXILLARY (AX) 3208 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1601 ANIMAL NUMBER: C91460 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/30/99 STUDY DAY OF DEATH: 429 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/30/99 14:23 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 59; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; SWOLLEN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY DARK; ALL LOBES: BROWN KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL SPLEEN (SP) : -LARGE; 62 X 11 X 8 MM HISTOPATHOLOGY LIVER (LI) : -PIGMENT, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE, MULTI FOCAL ADRENAL, CORTEX (AC) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE, FOCAL JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 3209 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1602 ANIMAL NUMBER: C91460 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/30/99 STUDY DAY OF DEATH: 429 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/30/99 14:23 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CERVIX (CV) : -MASS(ES); SINGLE MASS, 75 X 50 X 45 MM (116.0 GRAMS), RED, DARK RED, TAN, BROWN, FIRM AND LOBULATED; ON SECTION APPEARS FIBROUS; COLLECTED; POSSIBLY CONSISTENT WITH MASS AS NOTED IN CLINICAL OBSERVATIONS; REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL LUMBAR: EACH IS 8 X 3 X 2 MM (CAP 20) GENERAL COMMENT (GC) : -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DARK BROWN AND DRY MATERIAL -ANIMAL THIN CERVIX (CV) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT VAGINA (VA) : >AUTOLYTIC CAUSE OF DEATH (CD) : -ENDOMETRIAL STROMAL POLYP,-PRESENT LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL 3210 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1603 ANIMAL NUMBER: C91460 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/30/99 STUDY DAY OF DEATH: 429 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/30/99 14:23 PROSECTOR: DENNIS HOFFMAN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), UTERUS (UT), HEMATO NEOPLASIA (HN) 3211 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1604 ANIMAL NUMBER: C91461 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 345.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 51; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 84; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 10 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -PYELONEPHRITIS,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 3212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1605 ANIMAL NUMBER: C91461 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 345.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC REGION: MULTIPLE DARK RED CRUSTED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED WITH ALOPECIA -ALOPECIA-DIFFUSE; ENTIRE DORSAL THORACIC REGION (CAP 22) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL ABDOMINAL REGION: SINGLE MASS, 31 X 25 X 9 MM, TAN, GRAY, DARK RED, LOBULATED AND CYSTIC; SOME SECTIONS EXUDE A TAN AND CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE INGUINAL) -MASS #2; RIGHT VENTRAL ABDOMINAL REGION: SINGLE MASS, 29 X 23 X 14 MM, TAN, BROWN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A TAN AND CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE INGUINAL) LN, INGUINAL (IN) : -LARGE; LEFT: 11 X 6 X 6 MM (RIGHT NOT IDENTIFIED) (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN SKIN (SK) : -ATROPHY, ADNEXAL,-MODERATE, MULTI FOCAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL -ULCERATION,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING >NOTE:>INGUINAL LYMPH NODE DESCRIBED GROSSLY WAS A MAMMARY ADENOMA 3213 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1606 ANIMAL NUMBER: C91461 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/17/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 345.4 GRAMS DATE AND TIME OF NECROPSY: 09/17/99 15:09 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3214 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1607 ANIMAL NUMBER: C91462 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:08 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); ALL REGIONS, DIFFUSE: MULTIPLE RED AREAS, UP TO 2 X 1 MM -LARGE; 6 X 6 X 4 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3215 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1608 ANIMAL NUMBER: C91462 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 481.4 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:08 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) 3216 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1609 ANIMAL NUMBER: C91463 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 66; 25 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 71; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 9 MM, FIRM, RED, DARK RED AND TAN MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 13 X 23 X 37 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 23 X 34 X 37 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 23); REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -CYST,-PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 3217 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1610 ANIMAL NUMBER: C91463 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE 3218 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1611 ANIMAL NUMBER: C91463 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 608 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 363.3 GRAMS DATE AND TIME OF NECROPSY: 09/25/99 13:17 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, AXILLARY (AX) 3219 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1612 ANIMAL NUMBER: C91464 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 304.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 35; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LARGE; 6 MM IN DIAMETER; APPEARS SWOLLEN HISTOPATHOLOGY LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -INFARCT,-SLIGHT, MULTI-FOCAL -MINERALIZATION, TUBULAR,-SLIGHT, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 3220 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1613 ANIMAL NUMBER: C91464 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 304.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION, EXTENDING TO MID PERIANAL REGION: SINGLE MASS, 46 X 37 X 24 MM, TAN, LOBULATED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; OVERLYING SKIN HAS A SINGLE MOIST RED ULCERATED AREA, 14 X 11 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (INGUINAL) NOT IDENTIFIED AORTA (AO) : -FIRM; DIFFUSE GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN CAUSE OF DEATH (CD) : -MAMMARY CARCINOMA,-PRESENT AORTA (AO) : -MINERALIZATION,-MODERATELY SEVERE, DIFFUSE 3221 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1614 ANIMAL NUMBER: C91464 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 512 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 304.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3222 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1615 ANIMAL NUMBER: C91465 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 221.9 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:07 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; DEHYDRATED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK RED, TAN, AND SOFT MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3223 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1616 ANIMAL NUMBER: C91465 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 221.9 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:07 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERINEUM: YELLOW AND MOIST -BLOOD FILM TAKEN -MALOCCLUSION; LEFT UPPER INCISOR -ANIMAL THIN -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: BROKEN OFF AT BASE 3224 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1617 ANIMAL NUMBER: C91465 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/11/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: 221.9 GRAMS DATE AND TIME OF NECROPSY: 10/11/99 14:07 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3225 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1618 ANIMAL NUMBER: C91466 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 451.7 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN PELVIS: MULTIPLE TAN, FIRM GRANULES, PINPOINT AND UP TO 1 MM IN DIAMETER -LIGHT FOCUS(I)/AREA(S); LEFT, AT CRANIAL END: SINGLE TAN, SLIGHTLY RAISED AREA, 6 X 5 X 4 MM OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -B-LIPOMA,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT 3226 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1619 ANIMAL NUMBER: C91466 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 451.7 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY BONE, FEMUR (FE) : >NOTE:>TRIMMING COMMENT: MISSING AT TIME OF TRIMMING MARROW, FEMUR (FM) : >NOTE:>TRIMMING COMMENT: MISSING AT TIME OF TRIMMING PITUITARY (PI) : -MASS(ES); 8 X 7 X 7 MM, DARK RED, GREY AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 X 2 MM VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MUCOID SEMIFLUID MATERIAL FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DRY, BROWN CRUSTED AREA, 9 X 7 MM; COLLECTED BONE, FEMUR (FE) : >TISSUE MISSING MARROW, FEMUR (FM) : >TISSUE MISSING LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD(S) (FP) : -FIBROSIS,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 3227 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1620 ANIMAL NUMBER: C91466 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 451.7 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -STAINS - SKIN/HAIRCOAT; INVOLVING ENTIRE HEAD: DRY, RED MATERIAL 3228 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1621 ANIMAL NUMBER: C91466 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/03/99 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 451.7 GRAMS DATE AND TIME OF NECROPSY: 11/03/99 13:40 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 3229 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1622 ANIMAL NUMBER: C91467 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 467.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:40 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SCALY SKIN; TAIL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; LIMBS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 7 X 5 MM, DARK RED, GREY, AND FIRM LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 20) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL TAIL (TI) : -EROSION,-SLIGHT, MULTI-FOCAL 3230 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1623 ANIMAL NUMBER: C91467 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 467.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:40 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TAIL (TI) : -CRUSTED AREA(S); ALONG ENTIRE LENGTH: MULTIPLE TAN, DRY, CRUSTED AREAS, UP TO 1 MM IM DIAMETER; COLLECTED FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HIND FEET: SINGLE CRUSTED AREA ON EACH; LEFT IS 6 X 5 MM, RED, TAN, AND DRY; RIGHT IS 2 MM IN DIAMETER, TAN AND DRY; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN TAIL (TI) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL FOOT/FOOTPAD(S) (FP) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL 3231 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1624 ANIMAL NUMBER: C91467 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 467.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:40 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), LUNG (LU), URINARY BLADDER (UB) 3232 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1625 ANIMAL NUMBER: C91468 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/12/99 11:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, PINPOINT AND UP TO 6 X 3 MM HISTOPATHOLOGY LIVER (LI) : -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : >UNREMARKABLE PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT JEJUNUM (JE) : -CYST, KERATIN,-SEVERE, FOCAL 3233 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1626 ANIMAL NUMBER: C91468 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/12/99 11:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY JEJUNUM (JE) : -MASS(ES); MID AND DISTAL JEJUNUM, SEROSAL SURFACE: TWO MASSES, MID IS 10 X 9 X 7 MM, DISTAL IS 15 X 12 X 11 MM, TAN, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MESENTERIC) NORMAL UTERUS (UT) : -LUMEN FILLED WITH FLUID; BILATERAL: LIGHT GREEN, CLOUDY FLUID -LARGE, DIFFUSE; BILATERAL: EACH IS UP TO 20 MM IN DIAMETER CAVITY, THORACIC (TA) : >NOTE:>PERICARDIAL SAC AND AORTIC ARCH LACERATED; HEART FOUND FLOATING FREE WITHIN CAVITY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -FLUID-THORACIC CAVITY; DARK RED, GELATINOUS, SEMIFLUID UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE CAUSE OF DEATH (CD) : -KERATIN CYST,-PRESENT CAVITY, THORACIC (TA) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL 3234 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1627 ANIMAL NUMBER: C91468 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 04/12/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 04/12/99 11:40 PROSECTOR: STEVE VAN ADESTINE RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3235 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1628 ANIMAL NUMBER: C91469 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/09/99 8:40 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -PIGMENT, INCREASED,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SEVERE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, MULTI-FOCAL -HEMORRHAGE,-SEVERE, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL 3236 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1629 ANIMAL NUMBER: C91469 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/09/99 8:40 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); SINGLE MASS, 11 X 7 X 5 MM, RED, DARK RED, TAN, LOBULATED, AND SOFT GENERAL COMMENT (GC) -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3237 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1630 ANIMAL NUMBER: C91469 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/09/99 STUDY DAY OF DEATH: 500 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/09/99 8:40 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3238 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1631 ANIMAL NUMBER: C91470 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:45 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AND FIRM FOCI, PINPOINT AND UP TO 1 MM IN DIAMETER -MOTTLED; MARGIN OF LEFT LATERAL LOBE: SINGLE AREA, 28 X 13 MM, AREA APPEARS MOTTLED, RED, TAN AND FIRM; COLLECTED PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 3239 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1632 ANIMAL NUMBER: C91470 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:45 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 8 X 5 MM -THICKENED WALL; MUCOSA, DIFFUSE VAGINA (VA) : -SEMIFLUID MATERIAL; TAN AND MUCOID GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK BROWN AND DRY -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -STAINS - SKIN/HAIRCOAT; ABDOMEN AND CHEST: RED AND DRY -GI TRACT-DARK DRY CONTENT; CECUM: DARK BROWN SEMIFLUID STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -ULCERATION,-MODERATELY SEVERE, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3240 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1633 ANIMAL NUMBER: C91470 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/17/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 05/17/99 15:45 PROSECTOR: NANCY DIEDRICH RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) (SM), 3241 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1634 ANIMAL NUMBER: C91471 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 297.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED, DARK RED, TAN AND BROWN KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ANTERIOR ASPECT: SINGLE RED AREA, 2 X 1 MM SALIV GL, MANDIB (SG) : >NOTE:>TRIMMING COMMENT: MANDIBULAR SALIVARY GLANDS ARE BOTH MISSING HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL OVARY (OV) : -CYST,-PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : >TISSUE MISSING MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 3242 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1635 ANIMAL NUMBER: C91471 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 297.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT LATERAL CERVICAL REGION: SINGLE MASS, 12 X 10 X 8 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL; COLLECTED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED CAUSE OF DEATH (CD) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT 3243 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1636 ANIMAL NUMBER: C91471 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 675 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 297.7 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 13:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) 3244 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1637 ANIMAL NUMBER: C91472 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 674.9 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:21 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 67; 100 X 115MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL ABDOMINAL REGION: SINGLE MASS, 115 X 105 X 45 MM (300.2 GRAMS), TAN, RED, FIRM, LOBULATED AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, MULTI-FOCAL -B-ADENOMA,- 2 NEOPLASMS PRESENT MAMMARY, FEMALE (MF) : -B-ADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -MAMMARY FIBROADENOMA,-PRESENT 3245 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1638 ANIMAL NUMBER: C91472 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/06/99 STUDY DAY OF DEATH: 680 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 674.9 GRAMS DATE AND TIME OF NECROPSY: 12/06/99 12:21 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MEs /pANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3246 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1639 ANIMAL NUMBER: C91473 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 87; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); CAUDATE LOBE: SINGLE TAN AREA, 2 MM IN DIAMETER; COLLECTED PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, DARK RED, GRAY AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, NEAR JUNCTION WITH NONGLANDULAR MUCOSA: SINGLE RED ERODED AREA, 2 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATE, FOCAL >NOTE:>MINIMAL FOCAL EOSINOPHILIC FOCUS, MINIMAL FOCAL CYSTIC DEGENERATION, MINIMAL MULTIFOCAL VACUOLATION KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 3247 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1640 ANIMAL NUMBER: C91473 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 20 X 15 X 10 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3248 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1641 ANIMAL NUMBER: C91473 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 494.6 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 10:45 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB), LN, AXILLARY (AX) 3249 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1642 ANIMAL NUMBER: C91474 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 315.2 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:46 PROSECTOR: NANCY DIEDRICH RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 10 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 10 X 10 X 8 MM, RED, DARK RED, GREY AND SOFT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERINEAL REGION: SINGLE MASS, 30 X 25 X 15 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MUCOID SEMIFLUID MATERIAL HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL OVARY (OV) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE 3250 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1643 ANIMAL NUMBER: C91474 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 315.2 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:46 PROSECTOR: NANCY DIEDRICH RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY TONGUE (TO) : -MASS(ES); VENTRAL SURFACE, AT BASE: SINGLE MASS, 3 MM IN DIAMETER, TAN AND SOFT; ON SECTION EXUDES A TAN GRANULAR SEMISOLID MATERIAL; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND CRUSTED -MALOCCLUSION; BILATERAL, UPPER INCISORS CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT TONGUE (TO) : -CYST,-PRESENT 3251 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1644 ANIMAL NUMBER: C91474 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 315.2 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:46 PROSECTOR: NANCY DIEDRICH RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) 3252 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1645 ANIMAL NUMBER: C91475 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; LIMBS-HIND; VERIFIED AT NECROPSY: OBSERVED >EYES; CLEAR DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND TAN SPLEEN (SP) : -SMALL; 32 X 9 X 6 MM ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 X 10 X 9 MM (LEFT IS 6 X 5 X 4 MM) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL SPLEEN (SP) : >UNREMARKABLE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MODERATELY SEVERE, MULTI FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : -CYST,-PRESENT 3253 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1646 ANIMAL NUMBER: C91475 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 11 X 10 X 9 MM, DARK RED, TAN, AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 MM IN DIAMETER UTERUS (UT) : -MASS(ES); INVOLVING ENTIRE LEFT HORN: SINGLE MASS, 45 X 17 X 17 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CONTAINS A DARK RED SEMIFLUID LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : >UNREMARKABLE UTERUS (UT) : -DILATATION,-SEVERE, DIFFUSE -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL -NECROSIS,-MODERATE, MULTI-FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3254 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1647 ANIMAL NUMBER: C91475 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); ATTACHED TO ADIPOSE TISSUE CRANIAL TO RIGHT OVARY AND MEDIAL TO RIGHT UTERINE HORN: TWO MASSES, ONE IS 15 X 10 X 5 MM, THE OTHER IS 7 X 6 X 5 MM, BOTH ARE YELLOW AND FIRM; BOTH ARE SAME ON CUT SURFACE (CAP 22); REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 7 X 7 X 5 MM (CAP 23) FOOT/FOOTPAD(S) (FP) : -CRUSTED AREA(S); BILATERAL HIND FEET: SINGLE DARK RED AND DRY AREA ON EACH, LEFT IS 5 MM IN DIAMETER, RIGHT IS 7 MM IN DIAMETER; BOTH COLLECTED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DARK RED AND MOIST -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY CAVITY, ABDOM (PC) : -NECROSIS, FAT,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL FOOT/FOOTPAD(S) (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL 3255 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1648 ANIMAL NUMBER: C91475 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/23/99 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 523.0 GRAMS DATE AND TIME OF NECROPSY: 12/23/99 7:54 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), HEMATO NEOPLASIA (HN) 3256 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1649 ANIMAL NUMBER: C91476 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/05/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 08/05/99 15:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 4 X 4 X 6 MM (RIGHT IS 3 X 4 X 5 MM) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 7 X 11 MM, SOFT, RED, DARK RED AND TAN MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED WITH ROUTINE SECTION HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -VACUOLATION,-MODERATE, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3257 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1650 ANIMAL NUMBER: C91476 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/05/99 STUDY DAY OF DEATH: 557 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 08/05/99 15:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3258 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1651 ANIMAL NUMBER: C91477 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 296.1 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 46; 60 X 70MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 10 MM SOFT, RED, DARK RED AND TAN MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERIANAL REGION: SINGLE MASS, 33 X 50 X 68 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, MULTI-FOCAL LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE 3259 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1652 ANIMAL NUMBER: C91477 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 296.1 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOID MATERIAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST YELLOW MATERIAL -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3260 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1653 ANIMAL NUMBER: C91477 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/13/99 STUDY DAY OF DEATH: 596 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: 296.1 GRAMS DATE AND TIME OF NECROPSY: 09/13/99 15:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), HEMATO NEOPLASIA (HN) 3261 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1654 ANIMAL NUMBER: C91478 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 13:52 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 78; 70 X 70MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSALTHORACIC-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADCRANIAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL -THROMBUS,-MODERATE, FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL -THROMBUS,-MODERATELY SEVERE, FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LUNG (LU) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL 3262 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1655 ANIMAL NUMBER: C91478 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 13:52 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 70 X 50 X 35 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY); OVERLYING SKIN HAS A SINGLE RED AND DARK RED CRUSTED AREA, 55 X 34 MM; COLLECTED INTACT WITH MASS PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC RECTUM (RE) : >TISSUE MISSING SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL CAUSE OF DEATH (CD) : -MALIGNANT FIBROUS HISTIOSARCOMA,PRESENT HEMATO NEOPLASIA (HN) : -M-MALIGNANT FIBROUS HISTIOCYTOMA,NEOPLASM PRESENT 3263 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1656 ANIMAL NUMBER: C91478 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 13:52 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; LEFT: 34 X 17 X 8 MM (RIGHT IS 4 X 3 X 2 MM) (CAP 21) GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: RED AND DRY MATERIAL LN, AXILLARY (AX) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 3264 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1657 ANIMAL NUMBER: C91478 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/10/99 STUDY DAY OF DEATH: 593 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/10/99 13:52 PROSECTOR: TONY HANFELD RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), BRAIN (BR), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA) 3265 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1658 ANIMAL NUMBER: C91479 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AREA, 15 X 3 MM; COLLECTED ON ROUTINE SECTION -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LIVER (LI) : -FIBROSIS,-SLIGHT, MULTI-FOCAL -TELANGIECTASIS,-SLIGHT, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3266 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1659 ANIMAL NUMBER: C91479 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 596.2 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB) 3267 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1660 ANIMAL NUMBER: C91480 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED SECTIONS PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, DARK RED, TAN, GREY, AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL CERVIX (CV) : -B-LEIOMYOMA,-NEOPLASM PRESENT 3268 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1661 ANIMAL NUMBER: C91480 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY CERVIX (CV) : -MASS(ES); EXTENDING INTO VAGINA: SINGLE MASS, 12 X 10 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (ILIAC) NORMAL (CAP 20) UTERUS (UT) : -CYST(S); MID PORTION OF RIGHT HORN: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN UTERUS (UT) : -DILATATION,-MODERATE, FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : >UNREMARKABLE >NOTE:>ILIAC LYMPH NODE 3269 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1662 ANIMAL NUMBER: C91480 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 421.5 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:49 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), LUNG (LU), URINARY BLADDER (UB) 3270 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1663 ANIMAL NUMBER: C91481 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 61; 50 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER THYMUS (TH) : >NOTE:>TRIMMING COMMENT: MISSING AT TRIMMING HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -I-PITUITARY CARCINOMA,-NEOPLASM PRESENT KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL OVARY (OV) : -CYST,-PRESENT THYMUS (TH) : >TISSUE MISSING LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3271 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1664 ANIMAL NUMBER: C91481 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED AND SOFT SKIN (SK) : >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MAMMARY MASS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION, EXTENDING TO LEFT PERIANAL REGION: SINGLE MASS, 70 X 50 X 25 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS MULTIPLE DARK RED AND DRY ABRADED AREAS, UP TO 8 MM IN DIAMETER; COLLECTED INTACT WITH MASS >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MAMMARY MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -M-CARCINOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, MULTI-FOCAL SKIN (SK) : -EDEMA,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, -ULCERATION,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -M-CARCINOMA,-NEOPLASM PRESENT FOCAL CAUSE OF DEATH (CD) : -PITUITARY CARCINOMA,-PRESENT 3272 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1665 ANIMAL NUMBER: C91481 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/18/99 STUDY DAY OF DEATH: 509 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 303.6 GRAMS DATE AND TIME OF NECROPSY: 06/18/99 14:45 PROSECTOR: KEVIN BILLINGS RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3273 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1666 ANIMAL NUMBER: C91482 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 281.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:45 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 72; 30 X 35MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,-SEVERE, FOCAL 3274 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1667 ANIMAL NUMBER: C91482 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 281.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:45 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: TWO MASSES, ONE IS 40 X 30 X 20 MM, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; THE OTHER IS 20 X 18 X 10 MM, RED, DARK RED, TAN AND SOFT; ON SECTION EXUDES A DARK RED CREAMY SEMIFLUID; BOTH COLLECTED INTACT; REGIONAL LN (INGUINAL) NORMAL (CAP 21) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN SKIN (SK) : -ULCERATION,-SLIGHT, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT CERVIX (CV) : -DILATATION,-MODERATE, FOCAL -B-POLYP, ENDOMETRIAL STROMAL,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : >TISSUE MISSING 3275 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1668 ANIMAL NUMBER: C91482 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 281.6 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:45 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3276 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1669 ANIMAL NUMBER: C91483 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 11:05 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; LIMB-HIND-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -LARGE; 7 X 5 X 3 MM; APPEARS SWOLLEN HISTOPATHOLOGY EYE (EY) : -INFLAMMATION, CHRONIC-ACTIVE, CORNEAL,MINIMAL, MULTI-FOCAL -VASCULARIZATION, CORNEAL,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -VACUOLATION, WHITE MATTER,-MODERATE, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 3277 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1670 ANIMAL NUMBER: C91483 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 11:05 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL -LARGE; BILATERAL: EACH IS 10 X 6 X 3 MM; COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-ORAL; DARK RED AND DRY -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY MAMMARY, FEMALE (MF) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, DIFFUSE 3278 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1671 ANIMAL NUMBER: C91483 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/22/99 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/22/99 11:05 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 3279 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1672 ANIMAL NUMBER: C91484 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 186.1 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:29 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 9 X 8 MM, DARK RED, GRAY, LOBULATED, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT 3280 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1673 ANIMAL NUMBER: C91484 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/31/99 STUDY DAY OF DEATH: 491 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 186.1 GRAMS DATE AND TIME OF NECROPSY: 05/31/99 10:29 PROSECTOR: JOHN S. HALFORD RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3281 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1674 ANIMAL NUMBER: C91485 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 5 MM IN DIAMETER PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); ANTERIOR ASPECT: SINGLE BLACK AREA, 5 X 3 MM MAMMARY, FEMALE (MF) : -CYST(S); RIGHT PERINEAL REGION: MULTIPLE WHITE, TAN, AND CREAMY, SEMISOLID FILLED CYSTS, UP TO 15 MM IN DIAMETER; (CAP 20) HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 3282 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1675 ANIMAL NUMBER: C91485 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: DARK RED, AND TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN OFF AT BASE CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 3283 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1676 ANIMAL NUMBER: C91485 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.8 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 11:01 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: URINARY BLADDER (UB) 3284 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1677 ANIMAL NUMBER: C91486 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 261.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:00 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; OPAQUE; EYE-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MISSING; TAIL-PROXIMAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; SWOLLEN; MOUTH; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY EYE (EY) : -OPAQUE CORNEA; RIGHT BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY EYE (EY) : -SYNECHIA, ANTERIOR,-PRESENT -VASCULARIZATION, CORNEAL,-SLIGHT, FOCAL >NOTE:>RIGHT EYE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, MULTI-FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3285 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1678 ANIMAL NUMBER: C91486 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 261.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:00 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 MM IN DIAMETER, DARK RED, TAN, AND SOFT LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 12 X 8 X 3 MM; (CAP 21) LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS UP TO 11 X 6 X 3 MM (CAP 22) LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS UP TO 10 X 4 X 3 MM (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -TAIL - BENT/MISSING; TIP: MISSING, PORTION OF REMAINING TAIL COLLECTED PITUITARY (PI) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL LN, LUMBAR/ILIAC (LM) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL LN, RENAL (RL) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL 3286 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1679 ANIMAL NUMBER: C91486 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 640 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 261.2 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 15:00 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: SPLEEN (SP), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3287 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1680 ANIMAL NUMBER: C91487 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:31 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 7 X 5 MM, RED, DARK RED, TAN, AND FIRM STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE RED AREAS, UP TO 1 MM IN DIAMETER HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL 3288 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1681 ANIMAL NUMBER: C91487 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:31 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY UTERUS (UT) : -CYST(S); WITHIN LUMEN OF LEFT HORN 14 MM FROM BIFURCATION: SINGLE DARK RED FLUID FILLED CYST, 4 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -ANIMAL OBESE URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL UTERUS (UT) : -DILATATION,-MODERATE, FOCAL -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 3289 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1682 ANIMAL NUMBER: C91487 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 682.7 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:31 PROSECTOR: JAMES T. CALLEN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), LIVER (LI), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAr /iLIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY) 3290 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1683 ANIMAL NUMBER: C91488 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 507.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER, COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER UTERUS (UT) : -CYST(S); DISTAL END OF LEFT HORN, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 8 X 5 X 5 MM HISTOPATHOLOGY LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL OVARY (OV) : >UNREMARKABLE LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, MULTI-FOCAL CAUSE OF DEATH (CD) : -SCHEDULED SACRIFICE,-PRESENT 3291 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1684 ANIMAL NUMBER: C91488 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: SCHEDULED, FIRST POST-RECOV SAC DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 732 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 507.0 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 13:09 PROSECTOR: GREGORY CODDINGTON RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, MANDIBULAR (MN) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), MAMMARY, FEMALE (MF), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON(TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) 3292 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1685 ANIMAL NUMBER: C91489 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 13:36 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 79; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY EYE (EY) : -WHITE INTRAOCULAR MATERIAL; LEFT LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 6 MM (COLLECTED WITH ROUTINE SECTIONS) PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 2 X 4 MM MAMMARY, FEMALE (MF) : -MASS(ES); LEFT CERVICAL REGION: SINGLE MASS, 11 X 20 X 25 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE (CAP 21); REGIONAL LN (MANDIBULAR) NORMAL (CAP 22) HISTOPATHOLOGY EYE (EY) : ^UNREMARKABLE LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL THYROID (TY) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 3293 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1686 ANIMAL NUMBER: C91489 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 13:36 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 23) GENERAL COMMENT (GC) : -FLUID-THORACIC CAVITY; FILLED WITH A DARK RED FLUID AND A DARK RED CLOTTED MATERIAL CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : >TISSUE MISSING LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 3294 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1687 ANIMAL NUMBER: C91489 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/09/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/09/99 13:36 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: BRAIN (BR), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: BRAIN (BR), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SKIN (SK), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3295 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1688 ANIMAL NUMBER: C91490 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:11 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK 75; 20 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT LIVER (LI) : >UNREMARKABLE KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT SKIN (SK) : -ULCERATION,-SEVERE, FOCAL 3296 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1689 ANIMAL NUMBER: C91490 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:11 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS O B S E R V A T I O N S (CONTINUED) NECROPSY HISTOPATHOLOGY SKIN (SK) : -MASS(ES); VENTRAL ABDOMINAL REGION: SINGLE MASS, 20 X 17 X 8 MM, TAN AND FIRM; ON SECTION, EXUDES A TAN CASEOUS SEMISOLID MATERIAL; OVERLYING SKIN HAS A DRY AND DARK RED CRUSTED AREA, 15 MM IN DIAMETER; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT VENTRAL THORACIC REGION: SINGLE MASS, 25 X 25 X 15 MM, TAN AND SOFT; SAME ON CUT SURFACE; OVERLYING SKIN HAS A MOIST, DARK RED AND CRUSTED ULCERATED AREA; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL OBESE MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,- 2 NEOPLASMS PRESENT CAUSE OF DEATH (CD) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL 3297 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1690 ANIMAL NUMBER: C91490 SEX: FEMALE DOSE GROUP: 6 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/23/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 417.4 GRAMS DATE AND TIME OF NECROPSY: 08/23/99 12:11 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: EYE (EY), KIDNEY (KD), SPLEEN (SP), PANCREAS (PA), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), THYROID (TY), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML), CAVITY, ABDOM (PC), CAVITY, ORAL (OC), LN, ANT MES/PANC (AP), LN, LUMBAR/ILIAC (LM), LN, RENAL (RL), LN, AXILLARY (AX), CALVARIUM/SKULL (CA), TAIL (TI), ANUS (AN), FOOT/FOOTPAD(S) (FP), LN, INGUINAL (IN), JOINT, OTHER (JT), ADIPOSE TISSUE (AT), AORTA (AO), AUDITORY SEB GL (AS), AORTIC ARCH (AA), LN, TRACHEOBRON (TB), URETER (UE), PINNA (PN), LN, POPLITEAL (PP), CAVITY, THORACIC (TA), SUBCUTANEOUS TIS (SQ), TONGUE (TO), DIAPHRAGM (DP), BILE DUCT (BD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: EYE (EY), PANCREAS (PA), ADRENAL, MEDULLA (MA), LN, MESENTERIC (MS), OVARY (OV), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), THYMUS (TH), LUNG (LU), HEART (HT), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), TRACHEA (TR), ESOPHAGUS (ES), PARATHYROID (PT), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), UTERUS (UT), VAGINA (VA), HEMATO NEOPLASIA (HN) 3298 APPENDIX 7 C e ll P ro life ra tio n R e p o rt Covance 6329-212 3M T-6316.1 N o te : T h is a p p en d ix c o n ta in s in fo rm a tio n su p p lied b y th e S p o n so r an d h as b e en re v ie w e d b y th e Q u a lity A ssu ra n ce U n it o f P a th o lo g y A sso c ia tes In te rn a tio n a l. 3299 Covance 6329-212 3M T-6316.1 3300 Study Number: Title: Date of Final Report: Amendments: Covance 6329-212 3M T-6316.1 :- ,**- PATHOLOGY ASSOCIATES A CHARLES RIVER COMPANY FIN A L R E PO R T A M EN D M EN TS Covance Study No. 6329-212 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats August 17, 1998 (1) Cover page: Header and company name changed to reflect new name. Company name also changed on page I1 , paragraph 2 in PURPOSE section. (2) Cover page: "FINAL" deleted. (3) Table of Contents page added; Section pages I-VI added. (4) Header changed globally to reflect section in the page number and to reflect amended final report. (5) PURPOSE: Paragraph added to reflect that Dr. Carolyn Moyer was unable to sign the final report and that Dr. Mark Butt signed in her place. (6) RESULTS: Reference to Tables and Figures changed to reflect Sections containing Tables and Figures. (7) RESULTS: Histopathology findings moved from Appendix I to Section IV. (8) Page 1-4, Line 5: Week 8 changed to week 14. (9) Page numbers changed to reflect section. (10) Tables: Removed "Pathology Associates International" from footer. (11) Signature page changed from Section IV to Section V. (12) QA Statement changed from Section V to Section VI. (13) Entire report: font changed. 3301 Covance 6329-212 3302 TABLE OF CONTENTS Report Narrative Tables Figures Histopathology Findings Signature Page Quality Assurance Statement Covance 6329-212 3M T-6316.1 Section I II III IV V VI 3303 Covance 6329-212 3M T-6316.1 I. REPORT NARRATIVE 3304 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Covance Study Number 6329-212 Page 1-1 C ELL PR O LIFER A TIO N R E PO R T FO R : 104-W EEK D IETA R Y C A R C IN O G EN IC ITY STUDY W IT H N A R R O W R A N G E (98.1% ) N -E T H Y L PE R FL U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A TS C O V A N C E STU D Y N U M B E R 6329-212 PURPOSE The purpose of the study was to assess the carcinogenicity of the test material when administered in the diet to rats for at least 104 weeks. This report, submitted by Pathology Associates - A Charles River Company (PAI), formerly Pathology Associates International, to the study Sponsor, 3M, represents the cell proliferation findings and interpretation for Covance Study Number 6329-212 entitled " 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol In Rats". All aspects of the tasks associated with PAI's portion of this study were conducted in compliance with the Food and Drug Administration Good Laboratory Practice (GLP) Regulations as set forth in Title 21 of the US Code of Federal Regulations, Part 58, issued December 22, 1978 (effective June 20, 1979), and with any applicable amendments. At the request of the Sponsor, the portion evaluated and reported by Dr. Carolyn Moyer has been signed by Dr. Mark Butt, General Manager and Director of Pathology at PAI. Dr. Moyer is currently on medical leave and unavailable for signature. M A TER IA LS AND M ETH O D S T issue C ollection fo r Cell P ro liferatio n Five animals per sex per group in groups 1 through 5 were sacrificed during week 4, five animals per sex per group in groups 1 and 5 were sacrificed during week 8, and five animals per sex per group in groups 1 through 4 were sacrificed during week 14 for hepatocellular proliferation. A section of the left lateral lobe of the liver from each of 5 rats per group (groups 1-5) was fixed and processed to paraffin block by Covance per protocol specifications. Tissue blocks were shipped to PAI for sectioning and staining. From each block, a slide was prepared for H&E evaluation and immunohistochemical detection of proliferating cell nuclear antigen (PCNA), a marker of cell proliferation. Im m u n o h isto ch em istry fo r Cell P ro liferatio n Sections of paraffin-embedded tissues were cut at 5 pm and placed on positively charged slides (Superfrost Plus, Fisher Scientific, Pittsburgh, PA) to ensure adhesion during processing for PCNA. Standard immunohistochemical methods were used to stain tissues for PCNA (PAI's SOP for immunohistochemistry). Briefly, tissue sections were incubated with a monoclonal antibody to PCNA (DAKO, lot #016, PAI No. A1723) and reagents required for the avidin-biotin 3305 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Covance Study Number 6329-212 Page 1-2 peroxidase (ABC Kit; PAI No. K 309 and K 321) method for the detection of the antigenantibody complex. PCNA expression in cells in all phases of the cell cycle (Gp S, G, and M) was localized by the chromagen 3,3'-diaminobenzidine (DAB; Sigma Chemical Co., lot #17H8927). Tissue sections were counterstained with hematoxylin. C ell P ro liferatio n M easu rem en ts Positive staining for PCNA was categorized based on cellular distribution and intensity of the brown to black reaction product that correlated with the different phases of the cell cycle (Eldridge et al., 1993). Uniform, dark brown to black nuclear staining was judged to be positive for S phase cells. A cell in the G, phase of the cell cycle had diffuse, stippled nuclear staining that was lighter than a cell in S phase. Cells in the G, phase exhibited distinct, diffuse cytoplasmic stippling with or without nuclear staining. Mitotic figures were conspicuous with diffuse cytoplasmic stippling. The percentage of hepatocytes in each phase of the cell cycle (labeling index, LI) was determined by scoring at least 3000 hepatocytes in 10 randomly selected fields per animal. In addition, a proliferating index (PI; G,+S+G2+M phases combined) was determined and expressed as a percentage. A negative control slide was included in the staining run and consisted of study tissue that was not incubated with the primary antibody. For cell proliferation evaluations, slides were first perused at low magnification (100X) to judge quality of staining, processing and sectioning, pattern of cell labeling (e.g., centrilobular or panlobular, extrahepatocellular proliferation, such as Kupffer cells, bile duct epithelium, endothelium), and histomorphologic changes. The later was further assessed by evaluating the serial H&E slide for each animal evaluated for cell proliferation. Cell proliferation was then quantified at higher magnification (200X) as described above. S tatistical A nalysis The Student's i-test (two-sided, unequal variance) was used to test for statistical significance in PI between control and treatment groups using Microsoft Excel version 5.0. A P value of 0.05 was judged to be statistically significant. RESULTS C ell P ro liferatio n Individual animal and group mean cell proliferation data are presented in Section II (Tables II-1 to III-4) and graphically in Section III (Figures III-1 and III-2. The percentage of proliferating hepatocytes (PI) was significantly increased only in male rats exposed to the highest dose of the test material at 4 weeks on study. 3306 Covance 6329-212 3M T-6316.1 H istopathology Amended Final Cell Proliferation Report Covance Study Number 6329-212 Page 1-3 Sections from the same tissue blocks used for preparation of PCNA-stained slides were stained with hematoxylin and eosin (H&E) for histopathologic evaluation to facilitate the interpretation of the immunostained slides. Individual animal findings and group summaries are presented in Section IV. W eek 4 Findings Histomorphologic evaluation revealed hepatocellular hypertrophy, central vein vasodilation, hematopoietic cell proliferation, and mononuclear cell infiltrates in the periductular connective tissue of the portal sections of the liver in all animals of all dose groups, including controls. The hepatocellular hypertrophy was characterized by enlargement of a single cell layer of hepatocytes around central veins in a centrilobular pattern and below the serosal surface of the livers. The cytoplasm of these enlarged cells was consistently foamy and/or lacy in appearance. The nuclei, however, appeared normal, in that no evidence of pyknosis, margination of chromatin, or other changes of nuclear degeneration were evident. Frequently, the veins around which these hepatocytes were located, were dilated and partially blood-filled. The livers of two male animals, C90984 and C91005, had narrow areas of focal necrosis extending from the serosal surface into the hepatocellular parenchyma. The centrilobular hepatocellular changes were considered to be associated with pentobarbital anesthesia. W eek 8 an d 14 Findings The results of histomorphologic evaluation showed hepatocellular hypertrophy in high-dose male rats and a subset of control male and female rats that were sacrificed at 8 weeks into the study. These changes were considered to be consistent with changes that occur with pentobarbital anesthesia, as seen in animals sacrificed at 4 weeks on study. The male and female animals from dose groups 2-4 and control animals from group 1 sacrificed at week 14 did not show these changes. The animals sacrificed at week 14 were euthanized under CO, anesthesia. Liver necrosis, ranging from focal to focally extensive, were noted in select animals from the male dose groups 4 (week 14) and 5 (week 8) and female dose group 5 (week 8). This change was not associated with concomitant significant inflammation, nor evidence of hepatocellular regeneration, suggestive of a lesion which occurred as a terminal event. D ISC U SSIO N In the present study, cell proliferation was measured within the liver of male and female rats from control, low dose, mid dose, mid-high dose, and high dose test material after four, eight or 14 weeks on study to identify a NOEL for N-ethyl perfluorooctanesulfonamido ethanol based on a cell proliferation mode of action. A statistically significant increase in cell proliferation, as determined by the proliferating index (PI) was observed only in male rats from the high dose group after 4 weeks on study. This increase in PI was not observed after 8 weeks on study in the high dose male rats, nor in lower dose groups of male rats after 14 weeks on study. Cell proliferation was not statistically significant in female rats at 4, 8 or 14 weeks on study. 3307 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Covance Study Number 6329-212 Page 1-4 Although statistical analysis did not reveal a significant cell proliferative response to the test material, biological significance is evident in individual animals. Biological significance for an individual animal is defined as a doubling in PI over controls and greater than the highest control value. Thus, the data are presented in terms of severity (fold increase over controls) and incidence (number of animals showing biological significance). As seen in text table 1, biologically significant cell proliferation is evident in 2/5 male rats at the high dose and 1/5 male rats at the mid-high dose after 4 weeks on study, and in 2/4 male rats at the high dose after 8 weeks on study. Although 1 male rat exhibited a cell proliferative response at the mid-high dose after 4 weeks on study, this response was not sustained as evidenced by the lack of a response at week 14. Therefore, the NOEL in male rats is judged to be the mid-high dose. In contrast to male rats, biologically significant cell proliferation was not evident in female rats at any dose after 4 or 8 weeks on study, as seen in text table 2. Therefore, the NOEL in female rats is judged to be the high dose. T ex t T a b le 1. B iologically S ig n ifican t Cell P ro life ra tio n in L iv e r o f M ale R ats Group Week 1 Severity1 Incidence' Week 8 and 14' Severity1 Incidence2 Low dose Mid dose Mid-high dose High dose <1 <1 <1 1.8 i) 5 <1 () 5 ............. <1.............. 1/5 <1 2/5 2.8 00/5 o5 2/4 'Fold increase over controls 2Number of animals with a PI double the mean control value and greater than the highest control animal "High dose animals sacrificed at week 8; all other dose groups sacrificed at week 14 T ext T ab le 2. Biologically S ignificant Cell P ro liferatio n in L iv er of F em ale R ats Group Week 4 Severity1 Incidence2 Week 8 and 143 Severity1 Incidence2 Low dose 1.4 0-5 <1 0/5 Mid dose <1 () - <1 n5 Mid-high dose <1 0/5 <1 0/5 High dose 1.2 o5 <1 0/5 `Fold increase over controls "Number of animals with a PI double the mean control value and greater than the highest control animal "High dose animals sacrificed at week 8; all other dose groups sacrificed at week 14 3308 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Covance Study Number 6329-212 Page 1-5 PCNA immunostaining has been reported to detect differential patterns of cell labeling in the liver of mitogen-treated rodents (Eldridge, et ah, 1993). In the present study, the cell proliferative response observed in male rats was panlobular in that there was no distinct pattern of cell labeling. Furthermore, the increased proliferative response, as detected by PCNA immunostaining, was localized to hepatocytes. Interestingly, a rare pattern of PCNA-labeling was observed in high dose males and females, which was not seen in any other groups. The staining was characterized by a granular cytoplasmic staining pattern localized to the hepatocytes around central veins and below the serosal surface of the livers. Although histomorphologic evaluation revealed changes in these two areas within animals from all treatment groups, including controls, the PCNA-labeling pattern was observed only in animals from the high dose group. The significance of this type of labeling pattern is not know; however, it has been speculated that it may represent mitochondrial DNA synthesis (unpublished observation). Recently, it has been shown that PCNA can cross-react with peroxisomal multifunctional protein (Mayer, et ah, 1998). Thus, it is likely that the centrilobular staining pattern observed in the high dose animals is associated with peroxisomes. The centrilobular hepatocellular hypertrophy and vasodilation were consistent with alterations that occur with phnobarbital anesthesia and concomitant vasodilation and vascular pooling. The subserosal hepatocellular changes, which were cytologically identical to those seen in the centrilobular regions of the liver are suggestive of a response by hepatocytes to intraperitoneal administration of phnobarbital. Finally, the presence of hematopoietic cell proliferation and mononuclear cell infiltration into the portal regions of the liver are common findings in rodent livers and were considered to be incidental findings reflective of normal physiological events. SUM M ARY The histopathologic changes seen after 4 weeks on study were observed in equal incidence and severity across dose groups, including controls. It is assumed that potential concomitant changes in cell proliferation are also equally represented among all groups. Therefore, cell proliferation effects at 4 weeks are judged to be related to test material, rather than due to phnobarbital anesthesia. Thus, based on the hepatocellular proliferation findings, the NOEL for N-ethyl perfluorooctanesulfonamido ethanol in liver of male and female rats is determined to be the mid high dose and high dose, respectively. L IT E R A T U R E C ITED Eldridge, S.R., Butterworth, B.E., Goldsworthy, T.L. (1993). Proliferating cell nuclear antigen: A marker for hepatocellular proliferation in rodents. E n v iro n H e a lth P e rsp e c 101: 211-218. Mayer, D., Forstner, K., Beier, K., and Volkl, A. (1998). Monoclonal antibodies against proliferating cell nuclear antigen cross-react with the peroxisomal multifunctional protein. A n a l B io c h e m 256: 135-137. 3309 Covance 6329-212 3M T-6316.1 II. TABLES 3310 COVANCE STUDY NO. 6329-212 TABLE II-1. CELL PROLIFERATION IN MALE RATS Group 1-C ontrol 1-C ontrol 1-C ontrol 1-C ontrol 1-C ontrol Week of Sex Study M4 M4 M4 M4 M4 2-Low 2-Low 2-Low 2-Low 2-Low M4 M4 M4 M4 M4 3-Mid 3-Mid 3-Mid 3-Mid 3-Mid M4 M4 M4 M4 M4 4-M id-H igh 4-Mid-High 4-Mid-High 4-Mld-High 4-Mld-Hlgh M M M M M 4 4 4 4 4 5-Hlgh 5-Hlgh 5-Hlgh 5-High 5-High M4 M4 M4 M4 M4 Animal Number C90719 C90725 C90732 C90753 C90775 Mean SEM C90789 C90814 C90817 C90833 C90834 Mean SEM C90846 C90853 C90866 C90890 C90891 Mean SEM C90935 C90939 C90942 C90961 C90966 Mean SEM C90984 C91005 C91024 C91033 C91037 Mean SEM Percent Gl Phase 0.11% 0.23% 0.15% 0.11% 0.16% 0.15% 0.02% 0.22% 0.08% 0.08% 0.18% 0,02% 0.11% 0.04% 0.07% 0.05% 0.10% 0.07% 0.08% 0.07% 0.01% 0.04% 0.03% 0.05% 0.34% 0.05% 0.10% 0.06% 0.24% 0.09% 0.02% 0.26% 0.20% 0.16% 0.05% Percent S Phase 0.11% 0.19% 0.10% 0.11% 0.16% 0.13% 0.02% 0.20% 0.05% 0.05% 0.11% 0.07% 0.09% 0.03% 0.11% 0.02% 0.05% 0.07% 0.00% 0.05% 0.02% 0.07% 0.03% 0.05% 0.23% 0.11% 0.10% 0.04% 0.19% 0.16% 0.17% 0.14% 0.12% 0.16% 0.01% ` Proliferating index== p e rc e n ta g e o f h e p a to c y te s in G1+S+G2+M phase Page II-1 Percent G2 Phase 0.02% 0.00% 0.02% 0.05% 0.05% 0.03% 0.01% 0.05% 0.03% 0.05% 0.02% 0.04% 0.04% 0.01% 0.02% 0.00% 0.00% 0.00% 0.10% 0.03% 0.02% 0.04% 0.00% 0.05% 0.13% 0.00% 0.04% 0.02% 0.24% 0.28% 0.12% 0.14% 0.17% 0.19% 0.03% 3311 Percent M Phase 0.00% 0.00% 0.02% 0.02% 0.02% 0.01% 0.01% 0.05% 0.03% 0.00% 0.11% 0.00% 0.04% 0.02% 0.02% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.03% 0.02% 0.00% 0.00% 0.01% 0.01% 0.02% 0.00% 0.02% 0.17% 0.12% 0.07% 0.03% Proliferating Index* 0.24% 0.42% 0.29% 0.30% 0.38% 0.33% 0.03% 0.51% 0.18% 0.18% 0.43% 0.13% 0.28% 0.08% 0.22% 0.07% 0.15% 0.13% 0.19% 0.15% 0.03% 0.15% 0.08% 0,16% 0.70% 0.16% 0.25% 0.11% 0.70% 0.53% 0.34% 0.71% 0.61% 0.58% 0.07% Covance 6329-212 3M T-6316.1 COVANCE STUDY NO. 6329-212 TABLE 11-2. CELL PROLIFERATION IN FEMALE RATS Group 1-C ontrol 1-C ontrol 1-C ontrol 1-C ontrol 1-C ontrol Week of Sex Study F4 F4 F4 F4 F4 2-Low 2-Low 2-Low 2-Low 2-Low F4 F4 F4 F4 F4 3-Mid 3-Mid 3-Mid 3-Mid 3-Mid F4 F4 F4 F4 F4 4-M id-H igh 4-M id-H igh 4-M id-H igh 4 -M id -H ig h 4 -M id -H ig h F F F F F 4 4 4 4 4 5-High 5-High 5-High 5-High 5-High F4 F4 F4 F4 F4 Animal Number C91135 C91143 C91165 C91169 C91171 Mean SEM C91198 C91205 C9222 C91230 C9237 Mean SEM C91251 C91272 C91285 C91287 C91291 Mean SEM C 9 3 1 6 C9137 C91333 C91345 C91350 Mean SEM C91423 C91427 C91430 C91443 C91448 Mean SEM Percent G l Phase 0.00% 0.37% 0.02% 0.09% 0.05% 0.11% 0.07% 0.18% 0.23% 0.05% 0.35% 0.02% 0.17% 0.06% 0 . 11% 0.00% 0.10% 0.00% 0.18% 0.08% 0.04% 0.11% 0.07% 0.03% 0.02% 0.02% 0.05% 0.02% 0.07% 0.14% 0.07% 0.05% 0.02% 0.07% 0.02% Percent S Phase 0.09% 0.20% 0.05% 0.09% 0.07% 0.10% 0.03% 0.09% 0.11% 0.14% 0.19% 0.05% 0.12% 0.02% 0.09% 0.00% 0.05% 0.10% 0.11% 0.07% 0.02% 0.02% 0.02% 0.00% 0.05% 0.09% 0.04% 0.02% 0.09% 0.14% 0.05% 0.07% 0.07% 0.08% 0.02% ` Proliferating index== pe rcentage o f h p a to c y te s in G1+S+G2+M phase P age 11-2 Percent G2 Phase 0.00% 0.10% 0.00% 0.12% 0.02% 0.05% 0.02% 0.09% 0.07% 0.05% 0.14% 0.02% 0.07% 0.02% 0.00% 0.00% 0.12% 0.00% 0.09% 0.04% 0.03% 0.07% 0.05% 0.05% 0.00% 0.02% 0.04% 0.01% 0.16% 0.09% 0.26% 0.15% 0.07% 0.15% 0.03% 3312 Percent M Phase 0.00% 0.00% 0.00% 0.00% 0.05% 0.01% 0.01% 0.00% 0.00% 0.02% 0.00% 0.02% 0.01% 0.01% 0.02% 0.00% 0.02% 0.00% 0.02% 0.01% 0.01% 0.02% 0.00% 0.00% 0.00% 0.02% 0.01% 0.01% 0.02% 0.00% 0.05% 0.02% 0.00% 0.02% 0.01% Proliferating Index* 0.09% 0.67% 0.07% 0.30% 0.19% 0.27% 0.11% 0.36% 0.41% 0.26% 0.67% 0.12% 0.37% 0.09% 0.23% 0.00% 0.29% 0.10% 0.41% 0.21% 0.07% 0.22% 0.14% 0.08% 0.07% 0.16% 0.13% 0.03% 0.34% 0.37% 0.42% 0.30% 0.17% 0.32% 0.04% Covance 6329-212 3M T-6316.1 C O V AN C E STUDY NO. 6329-212 TABLE 111-3. CELL PROLIFERATION IN MALE RATS G ro u p 1-C o n tro l 1-C o n tro l 1-C o n tro l 1-C o n tro l 1-C o n tro l Sex M M M M M 5 -H lg h 5 -H lg h 5 -H lg h 5 -H lg h 5 -H lg h M M M M M 1-C o n tro l 1-C o n tro l 1-C o n tro l 1-C o n tro l 1-C o n tro l M M M M M 2-Low 2-Low 2-Low 2-Low 2-Low M M M M M 3 -M ld 3 -M ld 3 -M ld 3 -M ld 3 -M ld M M M M M 4-Mld-Hlgh 4-Mld-High 4-Mld-Hlgh 4-Mld-Hlgh 4 -M id -H lgh M M M M M W eek of Study 8 8 8 8 8 8 8 8 8 8 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 Anim al N um ber C90717 C90718 C90737 C90756 C90769 M ean SEM C90971 C90972 C90988 C90996 C91017 Mean SEM C90731 C90746 C90748 C90768 C90780 Mean SEM C90797 C90807 C90818 C90831 C90836 Mean SEM C90843 C90852 C90863 C90877 C90880 Mean SEM C90905 C90907 C90917 C90921 C90960 Mean SEM Percent G l Phase 0.03% 0.00% 0.03% 0.00% 0.00% 0.01% 0.01% ND 0.03% 0.00% 0.22% 0.08% 0.08% 0 .0 5 % 0.10% 0.02% 0.05% 0.00% 0.07% 0 .0 5 % 0 .0 2 % 0.03% 0.02% 0,02% 0.05% 0,00% 0.02% 0.01% 0.03% 0.00% 0.02% 0.03% 0.05% 0.03% 0.01% 0.00% 0.05% 0.00% 0.03% 0.05% 0.03% 0.01% Percent S Phase 0.00% 0.05% 0.05% 0.03% 0.02% 0.03% 0.01% ND 0.03% 0.03% 0,11% 0.06% 0.06% 0.02% 0.08% 0.02% 0.05% 0.00% 0.07% 0.04% 0.01% 0.03% 0.00% 0.07% 0.00% 0.00% 0.02% 0 .0 1 % 0.03% 0.00% 0.02% 0.00% 0.03% 0 .0 2 % 0 .0 1 % 0.03% 0.08% 0.03% 0,05% 0.08% 0 .0 5 % 0 .0 1 % 'P ro life ra tin g ln d e x = p e rc e n ta g e o f h e p a to c y te s in G l +S+G2+M p ha se ND, not d e te rm in e d d u e to p o o r section Percent G 2 Phase 0.03% 0.00% 0.00% 0.00% 0,00% 0.01% 0.01% ND 0.03% 0.00% 0.00% 0.00% 0.01% 0.01% 0,08% 0.00% 0.00% 0.03% 0.00% 0.02% 0.01% 0.00% 0.05% 0.05% 0.00% 0.02% 0.02% 0.01 % 0.00% 0.00% 0.02% 0.00% 0.03% 0.01% 0.01% 0.00% 0.00% 0.03% 0.03% 0.00% 0.01% 0.01% Percent M Phase 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% ND 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.03% 0.02% 0.02% 0.00% 0.02% 0.02% 0.00% 0.00% 0,00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.02% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0.00% 0 .0 0 % P ro life ra tin g In d e x ' 0.05% 0.05% 0.08% 0.03% 0.02% 0.05% 0.01% ND 0.08% 0,03% 0.33% 0.14% 0.14% 0.07% 0.28% 0.07% 0,12% 0.03% 0.17% 0.13% 0.04% 0.05% 0.07% 0.14% 0.05% 0.02% 0.07% 0.02% 0.05% 0.00% 0.10% 0.03% 0,11% 0.06% 0.02% 0.03% 0.14% 0.05% 0.11% 0.13% 0.09% 0.02% P ag e 11-3 3313 Covance 6329-212 3M T-6316.1 C O V A N C E STUDY N O . 6329-212 TABLE 11-4. CELL PROLIFERATION IN FEMALE RATS G roup 1-C o n tro l 1-C o n tro l 1-C o n tro l 1-C o n tro l 1-C o n tro l Sex F F F F F 5 -H lg h 5 -H ig h 5 -H lg h 5 -H ig h 5 -H ig h F F F F F 1-C o n tro l 1-C o n tro l 1-C o n tro l 1-C o n tro l 1-C o n tro l F F F F F 2-Low 2-Low 2-Low 2-Low 2-Low F F F F F 3-M id 3-M ld 3-M ld 3-M id 3-M ld F F F F F 4-M id-H lgh 4-M ld-H igh 4-M id-H igh 4-M id-H igh 4-M id-H igh F F F F F W eek of S tudy 8 8 8 8 8 8 8 8 8 8 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 14 A nim al Num ber C91126 C91140 C91145 C91162 C91163 M ean SEM C91393 C91394 C91404 C91415 C91438 M ean SEM C91129 C91155 C91160 C91174 C91181 M ean SEM C91192 C91223 C91233 C91241 C91248 M ean SEM C91281 C91288 C91293 C91299 C91304 M ean SEM C91329 C91337 C91355 C91370 C91379 M ean SEM P ercent G l Phase 0.00% 0.02% 0.00% 0.05% 0.05% 0 .0 2 % 0 .0 1 % 0.05% 0.00% 0.03% 0.00% 0.08% 0 .03% 0 .0 1 % 0.02% 0.05% 0.15% 0.00% 0.05% 0 .0 6 % 0 .0 3 % 0,02% 0.02% 0.02% 0.09% 0.02% 0 .0 4 % 0 .0 1 % 0.00% 0.00% 0.02% 0.00% 0.00% 0 .0 0 % 0 .0 0 % 0.03% 0.00% 0.00% 0.00% 0.00% 0 .0 1 % 0 .0 0 % P erc en t S Phase 0.02% 0.02% 0.02% 0.05% 0.05% 0 .0 3 % 0 .0 1 % 0.05% 0.03% 0.03% 0.03% 0.00% 0 .0 3 % 0 .0 1 % 0.00% 0.03% 0.13% 0.02% 0.00% 0 .0 4 % 0 .0 2 % 0.02% 0.00% 0.00% 0.05% 0,02% 0 .0 2 % 0 .0 1 % 0.05% 0.05% 0.00% 0.00% 0.00% 0.027o 0 .0 1 % 0.00% 0.00% 0.02% 0.00% 0.02% 0 .0 1 % 0 .0 1 % 'P ro life ra tin g in d e x = p e rc e n t a g e o f h p a to c y te s In G 1 + S + G 2 + M p h a s e P erc en t G 2 Phase 0.00% 0.00% 0.00% 0.05% 0.02% 0 .0 1 % 0 .0 1 % 0.00% 0.00% 0.00% 6.00% 0.00% 0 .0 0 % 0 .0 0 % 0.00% 0.00% 0.03% 0.10% 0.00% 0 .0 3 % 0 .0 2 % 0 .0 0 % 0 .0 0 % 0 .0 2 % 0 .0 0 % 0 .0 0 % 0 .1 0 % 0 .0 0 % 0.00% 0.00% 0.00% 0.00% 0.02% 0 .0 0 % 0 .0 0 % 0.00% 0.00% 0.00% 0.00% 0.00% 0 .0 0 % 0 .0 0 % P erc en t M Phase 0 .0 0 % 0.00% 0.00% 0.02% 0.00% 0 .0 0 % 0 .0 0 % 0.00% 0.00% 0 .0 0 % 0 .0 3 % 0 .0 0 % 0 .0 1 % 0 .0 1 % 0.00% 0.03% 0.10% 0.00% 0.00% 0 .0 3 % 0 .0 2 % 0.00% 0.00% 0.00% 0.00% 0.00% 0 .0 0 % 0 .0 0 % 0.00% 0,00% 0.00% 0.00% 0.00% 0 .0 0 % 0 .0 0 % 0.00% 0.00% 0.00% 0.00% 0.00% 0 .0 0 % 0 .0 0 % P roliferating in d ex* 0.02% 0.05% 0.02% 0.17% 0.11% 0 .0 8 % 0 .0 3 % 0.10% 0.03% 0.06% 0.05% 0.08% 0 .0 6 % 0 .0 1 % 0.02% 0.10% 0.41% 0.12% 0.05% 0 .1 4 /o 0 .0 7 % 0.05% 0.02% 0.04% 0.14% 0.05% 0 .0 6 % 0 .0 2 % 0.05% 0.05% 0.02% 0.00% 0.02% 0 .0 3 % 0 .0 1 % 0.03% 0.00% 0.02% 0.00% 0.02% 0 .0 1 % 0 .0 1 % P a g e 11-4 3314 Covance 6329-212 3M T-6316.1 Covance 6329-212 3M T-6316.1 III. FIGURES 3315 LABELING INDEX (% ) Covance 6329-212 3M T-6316.1 3316 LABELING INDEX (%) Covance 6329-212 3M T-6316.1 3317 Covance 6329-212 3M T-6316.1 IV. HISTOPATHOLOGY FINDINGS 3318 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-1 Individual A nim al F indings - M ale R ats W eek 4 of S tudy Animal Number Dose Group Sex______ Histologic Findings C90719 C90725 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 1 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild C90732 1 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild C90753 1 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild C90775 1 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild C90789 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3319 C90814 C90817 C90833 C90834 C90846 C90853 C90866 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-2 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 2 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 2 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 2 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3320 C90890 C90891 C90935 C90939 C90942 C90961 C90966 Covance 6329-212 3M T-6316.1 Male Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 PageIV-3 Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 4 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 4 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 4 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 4 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3321 C90984 C91005 C91024 C91033 C91037 Covance 6329-212 3M T-6316.1 Male Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-4 Liver, necrosis, focal, mild Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 5 Male Liver, necrosis, focal, mild Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 5 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 5 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 5 Male Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3322 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-5 Individual A nim al Findings - Fem ale W eek 4 o f Study Animal Number Dose Group C91135 C91143 1 C91165 1 C91169 1 C91171 1 C 9 1 198 Sex______ Histologic Findings Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3323 C91205 C91222 C91230 C91237 C91251 C91272 C91285 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-6 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 2 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 2 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 2 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3324 C91287 C91291 C91316 C91317 C91333 C91345 C91350 Covance 6329-212 3M T-6316.1 Female Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-7 Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3325 C91423 C91427 C91430 C91443 C91448 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-8 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 5 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 5 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 5 Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild Female Liver, centrilobular, hepatocellular hypertrophy, mild Liver, subserosa, hepatocellular hypertrophy, mild Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell, mild Liver, hematopoietic cell proliferation, mild 3326 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-9 Individual A nim al Findings - M ale R ats W eeks 8 an d 14 of S tudy Animal Number Dose Group Week Sex Histologic Findings C90717 1 8 Male Liver, centrilobular, hepatocellular hypertrophy, mild C90718 C90737 C90756 C90769 1 8 Male Liver, centrilobular, hepatocellular hypertrophy, mild 1 8 Male Liver, centrilobular, hepatocellular hypertrophy, mild 1 8 Male Liver, centrilobular, hepatocellular hypertrophy, mild 1 8 Male Liver, centrilobular, hepatocellular hypertrophy, mild C90731 1 14 Male No significant findings C90746 1 14 Male No significant findings C90748 1 14 Male No significant findings C90768 1 14 Male No significant findings C90780 1 14 Male No significant findings C90797 2 14 Male No significant findings C90807 2 14 Male No significant findings C90818 2 14 Male No significant findings C90831 2 14 Male No significant findings C90836 2 14 Male No significant findings C90843 3 14 Male No significant findings C90852 3 14 Male No significant findings 3327 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-10 C90863 3 14 Male No significant findings C90877 3 14 Male No significant findings C90880 3 14 Male No significant findings C90905 4 14 Male Liver, necrosis, focal C90907 4 14 Male Liver, necrosis, multifocal C90917 4 14 Male No significant findings C90921 4 14 Male No significant findings C90960 4 14 Male No significant findings C90971 5 8 Male Liver, centrilobular, hepatocellular hypertrophy, minimal Liver, necrosis, multifocal C90972 5 8 Male Liver, centrilobular, hepatocellular hypertrophy, minimal Liver, necrosis, multifocal C90988 5 8 Male Liver, centrilobular, hepatocellular hypertrophy, minimal Liver, necrosis, multifocal C90996 5 8 Male Liver, centrilobular, hepatocellular hypertrophy, minimal Liver, necrosis, multifocal C91017 5 8 Male Liver, centrilobular, hepatocellular hypertrophy, minimal 3328 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-11 In d iv id u al A nim al F indings - F em ale R ats W eeks 8 an d 14 of S tudy Animal Number Dose Group Week Sex Ehstologic Findings C91126 C91140 C91145 C91162 C91163 C91129 C91155 C91160 C91174 C91181 C91192 C91223 C91233 C91241 C91248 C91281 C91288 1 8 Female Liver, centrilobular, hepatocellular hypertrophy, minimal 1 8 Female Liver, centrilobular, hepatocellular hypertrophy, minimal 1 8 Female Liver, centrilobular, hepatocellular hypertrophy, mild 1 8 Female Liver, centrilobular, hepatocellular hypertrophy, mild 1 8 Female Liver, centrilobular, hepatocellular hypertrophy, mild 1 14 Female No significant findings 1 14 Female No significant findings 1 14 Female No significant findings 1 14 Female No significant findings 1 14 Female No significant findings 2 14 Female No significant findings 2 14 Female No significant findings 2 14 Female No significant findings 2 14 Female No significant findings 2 14 Female No significant findings 3 14 Female No significant findings 3 14 Female No significant findings 3329 C91293 C91299 C91304 C91329 C91337 C91355 C91370 C91379 C91393 C91394 C91404 C91415 C91438 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-12 3 14 Female No significant findings 3 14 Female No significant findings 3 14 Female No significant findings 4 14 Female No significant findings 4 14 Female No significant findings 4 14 Female No significant findings 4 14 Female No significant findings 4 14 Female No significant findings 5 8 Female Liver, necrosis, focal 5 8 Female No significant findings 5 8 Female No significant findings 5 8 Female No significant findings 5 8 Female Liver, necrosis, focally extensive 3330 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-13 Incidence of Histologic Findings - M ale Rats Week 4 of Study Histologic Findings Liver, centrilobular, hepatocellular hypertrophy Liver, subserosa, hepatocellular hypertrophy Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell Liver, hematopoietic cell proliferation Liver, necrosis, focal Group 1 Control Week 4 Group 2 Low Week 4 55 55 55 55 55 00 Group 3 Mid Week 4 Group 4 Mid-High Week 4 Group 5 High Week 4 555 555 555 5 55 555 002 3331 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV -14 Incidence of Histologic Findings - Fem ale Rats W eek 4 of Study Histologic Findings Liver, centrilobular, hepatocellular hypertrophy Liver, subserosa, hepatocellular hypertrophy Liver, central vein, vasodilation Liver, portal, infiltration, mononuclear cell Liver, hematopoietic cell proliferation Group 1 Control Week 4 Group 2 Low Week 4 Group 3 Group 4 Group 5 Mid Mid-High High Week 4 Week 4 Week 4 5 5 5 55 5 5 5 55 5 5 5 55 5 5 5 55 5 5 5 55 3332 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-15 Incidence of Histologic Findings - M ale Rats W eeks 8 an d 14 of Study Histologic Findings Group 1 Control Week 8 Group 1 Control Week 14 Group 2 Low Week 14 Group 3 Mid Week 14 Group 4 Mid-High Week 14 Group 5 High Week 8 Liver, centrilobular, hepatocellular 5 0 0 0 0 5 hypertrophy Liver, necrosis 0 000 2 4 3333 Covance 6329-212 3M T-6316.1 Amended Final Cell Proliferation Report Histopathology Findings Covance #6329-212 Page IV-16 Incidence of Histologic Findings - Fem ale Rats W eeks 8 and 14 of Study Histologic Findings Group 1 Control Week 8 Group 1 Control Week 14 Group 2 Low Week 14 Group 3 Mid Week 14 Group 4 Mid-High Week 14 Group 5 High Week 8 Liver, centrilobular, hepatocellular hypertrophy 5 0 00 0 0 Liver, necrosis 0 0 00 0 2 3334 V. SIG N A T U R E P A G E Covance 6329-212 3M T-6316.1 Submitted by: Project Manager: _____ Sandra R. Eldridge, Ph.D. ^ /0-fo-/ Date Project Pathologist: fi! hi 1 *Mark T. Butt, D.V.M., Diplomate, A.C.V.P. General Manager, Director of Pathology Pathology Associates - A Charles River Company Date *Carolyn Moyer, D.V.M., Diplomate, A.C.V.P. was the project pathologist, but was on medical leave and unavailable for signature. Therefore, the portion evaluated and reported by Dr. Moyer has been signed by Dr. Mark Butt, General Manager and Director of Pathology at PAI. 3335 Covance 6329-212 3M T-6316.1 IV. QUALITY ASSURANCE STATEMENT 3336 Covance 6329-212 3M T-6316.1 3337 APPENDIX 8 E le c tro n M ic ro sc o p y R e p o rt Covance 6329-212 3M T-6316.1 N o te : T h is a p p en d ix c o n ta in s in fo rm a tio n su p p lied b y th e P a th o lo g y A sso c ia tes In te rn a tio n a l an d h as b e e n re v ie w e d b y th e Q u a lity A ssu ra n c e U n it o f P a th o lo g y A sso c ia te s In tern a tio n a l. 3338 Covance 6329-212 3M T-6316.1 PATHOLOGY REPORT TRANSMISSION ELECTRON MICROSCOPY EVALUATION OF LIVER TISSUE IN RATS 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS COVANCE STUDY NUMBER 6329-212 PAI STUDY NUMBER EM 01.3 8 Prepared by: Connie A. Cummings, D.V.M., PhD. And James B. Nold, D.V.M., Ph.D., Diplomate, A.C.V.P. Pathology Associates A Charles River Company 4915-D Prospectus Drive Durham, NC 27713 October 10, 2001 PREPARED FOR COVANCE LABORATORIES INC., 3301 KINSMAN BOULEVARD MADISON, WISCONSIN 53704 SIGNATURE: THIS PAGE REVISED. COVANCE STUDY NO.:6329-212 ______ DATE: / / - / S ~ - / 3339 Covance 6329-212 3M T-6316.1 3340 TABLE OF CONTENTS Pathology Narrative Transmission Electron Micrograph Interpretations Transmission Electron Micrographs Quality Assurance Statement Covance 6329-212 3M T-6316.1 Section I II III IV 3341 SECTION I PATHOLOGY NARRATIVE Covance 6329-212 3M T-6316.1 3342 Covance 6329-212 3M T-6316.1 Sum m ary T en Crl.CD (S D ) IG S B R rats (five m ales and five fem ales) w ere treated fo r 104 w eeks w ith lOOppm o f 98.1% n-ethyl perfluorooctanesulfonam ido (N E tF O S E ). T en other rats o f sim ilar strain (five m ales and five fem ales) were used as controls. Liver sam ples were obtained from each o f the 20 rats during necropsy at term inal sacrifice for exam ination by transm ission electron m icroscopy (TEM ). L iver sam ples were assessed for changes that m ay likely occur due to chronic toxicity o f the test m aterial. Electron m icroscopic evaluation identified m ild to m oderate changes related to treatm ent. M ild to m oderate sm ooth endoplasm ic reticulum hyperplasia and minim al to mild hepatocellular hypertrophy were the prom inent features found to b e different between the m id-high dose group and control animals. O ther changes included a slight increase in the am ount o f lipid accum ulation and m inor increases in lipofuscin were found in the treated anim als com pared w ith controls. Procedure T he objective o f this study w as to exam ine u ltrastru ctu rally a sam ple o f liver from 10 rats treated orally fo r 104-w eeks w ith lOOppm o f N E tF O S E . H epatocytes w ere evaluated b y T E M for changes that m ay be related to the test m aterial. This study is p art o f a larger chronic toxicity study o f N E tF O S E . T his po rtio n o f the study included 10 treated rats (5 m ales and 5 fem ales) and 10 control rats (5 m ales and 5 fem ales). T reated rats w ere orally dosed daily for a t least 104 weeks w ith lOOppm o f N E tF O S E . T he control rats w ere given th e basal diet only. T erm inal necropsies w ere perform ed on all 20 rats. M ultiple tissues including liver w ere collected and preserved in 10% neutral-buffered form alin according to the study protocol. Additionally, sam ples o f liver from each anim al were thin-sliced, placed in 2% paraform aldehyde/2.5% glutaraldehyde in 0.1M phosphate buffer then subsequently processed and em bedded in epoxy blocks for exam ination by T E M . Thin sections (approxim ately 90nm ) from the epoxy blocks were cut, m ounted on 200-m esh copper grids, stained with 5 % methanolic uranyl acetate, and Reynold's lead citrate, then exam ined on a Zeiss EM 10C transm ission electron m icroscope. B oth centrilobular and periportal hepatocytes were examined. R epresentative ultrastructural photom icrographs from each liver sam ple were taken and significant features were described and recorded for each m icrograph, anim al, and each group. The descriptive features o f each rat were recorded on a transm ission electron m icrograph interpretation form . T he num ber o f peroxisom es per hepatocyte w as m anually counted then averaged for each anim al. The average num ber o f peroxisom es per group w as tabulated. R esults M icrograph interpretations for each rat can be found in section II. R epresentative m icrographs o f specific features are shown in section III as report figures. All rem aining electron m icrographs are separately attached to this report grouped according to rat number. T E M evaluation A total o f 108 m icrographs w ere evaluated. Fifty-four (54) m icrographs represented the control group while fifty-four (54) m icrographs represented the m id high-dose treated group. U ltra stru c tu ra l findings fo r e a c h g ro u p a re su m m arized in T a b le 1. T h ere w as a m inim al to m ild increase in the size o f hepatocytes in the treated group. Sim ilarly, a m ild to m oderate increase in sm ooth endoplasm ic reticulum (SER ) and a slightly m ild increase in lipid accum ulation w as also noted. The increase in the size o f m ost hepatocytes w as generally due to the increase in SE R and possibly the lipid as well. S E R is invariably affected during som e types o f hepatic toxicity, m ostly toxicities that induce hepatic m icrosom al enzymes and lipid peroxidation (Cheville, 1994). Sim ilarly, the increase in SER is regarded as an adaptive response by animals as a m eans to handle drug toxicities (G hadially, 1997). Based on personal com m unication w ith the study pathologist, liver w eights w ere increased and hepatocellular hypertrophy was diagnosed by light m icroscopy, w hich is consistent w ith ultrastructural findings o f an increase in SER (Ghadially, 3343 Covance 6329-212 3M T-6316.1 1997). One other m inor feature that w as prom inent in a few treated rats w as the accum ulation o f lysosom es that presum ably contained lipofUscin pigment. All control anim als were considered norm al. Although a sm all num ber o f control rats had a mild increase in SE R and a slight increase in the am ount o f lipid accum ulation, the increases in SER and lipid accum ulation in the treated anim als w as m uch m ore evident than in the control animals. Table 2 sum m arizes the quantitation o f peroxisomes for each group. Often, an increase in the num ber o f peroxisom es indicates an alteration in liver cell physiology. However, in this study, there was no difference in the average num ber o f peroxisom es between treated and untreated animals. T able 1 U ltrastru ctu ral findings p er anim al A nim al no G roupV S ex2 Com m ents C90716 1/M N orm al hepatocytes. C90722 1/M Increased S E R in 4/53 hepatocytes. C90724 1/M N orm al hepatocytes. C90726 1/M M ild lipid accum ulation. C90728 1/M N orm al hepatocytes. C 90904 4/M S E R is increased. H epatocellular hypertrophy in 4/6 hepatocytes. M oderate lipid accum ulation. C90908 4/M Increased SE R and lipid accum ulation also. C 90926 4/M Suboptim al fixation. Increased lysosom es m ost likely lipofUscin pigm ent. C 90927 4/M SER is increased. One hepatocyte is hypertrophic. C90928 C91127 4/M S E R is increased. Four out o f six hepatocytes are hypertrophic. 1/F N orm al hepatocytes. M ild lipid accum ulation. C91131 1/F N orm al hepatocytes. M ild lipid accum ulation. C91144 1/F N orm al hepatocytes. C91148 1/F N orm al hepatocytes. C91159 1/F N orm al hepatocytes. C91324 4/F SE R is increased. Both lipid and lysosom al accum ulation appear slightly increased as well. C91332 4/F M ild increase in SER. A few lysosom es observed as well. C91335 C91336 C91341 4/F SE R is increased. Three hepatocytes are slightly larger than norm al. 4/F S E R is increased. O ne hepatocyte is slightly hypertrophic. 4/F SE R is increased. Al --C ontrol, 4= M id -H ig h dose g ro u p ; 2M = m ale, F = fem ale; 3ra tio o f ab n o rm a l hepatocytes p er total num ber o f hepatocytes described. T able 2 Q uantitation of Peroxisom es p er ra t1 C ontrol G roup T reated A nim al # M ale A nim al # Fem ale Anim al # M ale C90716 11.3 C 91127 10.8 C909O4 7.3 C90722 20.0 C91131 10.8 C90908 9.7 C90724 22.5 C91144 12.8 C 90926 6.3 C90726 15.0 C 91148 11.5 C 90927 12.0 C90728 15.0 C 91159 11.0 C90928 11.0 M ean = 16.8 M ean = 11.4 M ean = 9.3 lrThe averag e n u m b er o f h ep ato cy tes co u n ted p e r ra t w as 5 G roup jfypm al # Fem ale C91324 8.5 C91332 8.7 C91335 12.4 C91336 10.4 C91341 9.8 M ean = 10.0 3344 Covance 6329-212 3MT-6316.1 Conclusion A n ultrastructural exam ination w as done on liver sam ples from rats treated orally w ith lOOppm o f N E tFO SE for a total o f a t least 104 weeks. T reatm ent related ultrastructural changes included a minimal to mild hepatocellular hypertrophy and a m ild to m oderate increase in SER. The am ount o f lipid accum ulation w as m inim ally increased in the treated anim als. Signature of Authors Diplom ate, A.C.V.P References Cheville N F: U ltrastructural Pathology A n Introduction to Interpretation. 1st ed. p. 704. Am es Iow a State University Press, 1994. G h a d ia lly F N : U ltrastru c tu ral p ath o lo g y o f th e cell an d m a trix . 3 rd ed. v. 1. p . 4 8 4 . London: Butterworths, 1997 3345 SECTION n Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATIONS 3346 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study N o.: 6329-212 E M 01.38 S p e c ies/S train :Crl:CD (SD ) IGS BR A n im a l N o .: C 9 0 7 1 6 ______ Tissue: Liver - Periportal T reatm ent G roup: 1 (Control! B lock N o(s).: C 90716-1A S e x : M a le _______ Photo/N egative N ofs).:L 6299-L6304 S ig n if ic a n t L e s io n s (c h e c k o n e ): __________ Y e s X No Interpreting Pathologist (signature and date): M arch 28, 2001 Features: L6299. Liver. Hepatocyte. 7,530X. To the left side o f the micrograph center, there is an oval shaped hepatocyte. The hepatocyte is somewhat smaller than normal. The nucleus is distinctly round and eccentrically positioned in the cell. The cytoplasm contains a number o f small round mitochondria, patches of RER and SER. Eleven (11) peroxisomes are counted. A large sinusoid is found on the right side o f the micrograph. Within this sinusoid are erythrocytes and some cellular debris. Some collagen fibrils are found in the upper right portion of this sinusoid. Other hepatocytes on this micrograph have similar characteristics as the described hepatocytes; however, a few have some small lipid droplets. L6300. Liver. Hepatocyte. 7,530X. In this micrograph, a number o f partial hepatocytes are observed. One whole hepatocyte is found in the center o f the micrograph. This cell has an eccentrically located round nucleus. The cytosol contains mitochondria, SER, and less RER. Seven (7) peroxisomes are counted. Below this hepatocyte, there is a perisinusoidal fat storing cell (Ito cell). Randomly scattered are erythrocytes and a prominent endothelial cell in the upper left hand comer of the micrograph. L6301. Liver. Hepatocyte. 10,20QX. A dark staining, oval hepatocyte is observed in the center o f the micrograph. There is a knife mark (artifact) running half way across the cell. The nucleus o f this cell is somewhat oval with coarse clumped chromatin. The cytosol contains SER, RER, and mitochondria. Ten (10) peroxisomes are counted. L6302. Liver. Hepatocyte. 7,920X. Portions o f two oval hepatocytes running obliquely across the micrograph are observed. The hepatocyte located in the center o f the micrograph is characterized by a smaller round nucleus. The cytoplasm o f this cell is composed o f areas o f SER separating mitochondria and some small cistemae of RER. Thirteen (13) peroxisomes are counted. The hepatocyte above this cell has similar characteristics but with a large nucleus. L6303. Liver. Hepatocyte. 7,920X. A polygonal-shaped hepatocyte is found in the center o f five other hepatocytes. This cell has a large distinctly rounded, eccentrically located nucleus. The cytoplasm is filled with abundant RER, mitochondria, and some SER. Two small lipid droplets are observed also. Eleven (11) peroxisomes are counted. O f the hepatocytes surrounding this cell, two contain medium round lipid droplets. L6304. Liver. Hepatocyte. 7,530X. In the center o f the micrograph is an oblong-shaped hepatocyte. This cell has a small round nucleus. The cytoplasm contains numerous mitochondria, RER, and SER. A few small to medium sized lipid droplets and randomly scattered lysosomes are also observed. Sixteen (16) peroxisomes are counted. A knife mark is found running across the micrograph (artifact).________________ C onclusions: N o rm a l hepatocytes. Peroxisom e average is 11.3 per hepatocyte._________________ 3347 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study N o.: 6329-212 E M 01.38 S pecies/S train: Crl:CD* tSD ) IGS BR A n im a l N o .: C 9 0 7 2 2 ______ Tissue: Liver -C entrilobular T reatm ent G roup: 1 (C ontrol) B lock N o(s).: C 90722 1A S e x : M a le _______ Photo/N egative N otsT :L 6208-L6212 Significant Lesions (check one): X Y es _______ N o Interpreting P athologist (signature and date): ( Ma r c h 2 6 , 2 0 0 1 Features: L6208. Liver. Hepatocyte. 10,200X. A somewhat cuboidal hepatocyte is found in the center of the micrograph. The hepatocyte has a distinctly rounded nucleus with a single nucleolus. The cytoplasm contains numerous mitochondria, RER, and SER. Twenty-five (25) peroxisomes are counted. Along the bottom right hand comer, there is a small amount o f chatter artifact). L6209. Liver. Hepatocyte. 10,200X. An oval hepatocyte is found in the center o f the micrograph. The nucleus is round with a m arginating nucleolus. The chromatin is finely granular. Within the cytosol, there are a number of mitochondria, RER, SER, and a few randomly scattered lipid droplets, some with myelin figures. Sixteen (16) peroxisomes are counted. L6210. Liver. Hepatocyte. 10,200X. A large somewhat oval hepatocyte is found vertically centered on the micrograph. The hepatocyte has a small nucleus (plane o f section) with dense coarse chromatin. The cytosol is filled with SER, patches o f RER, and numerous mitochondria. Twenty-two (22) peroxisomes are counted. Along the bottom left hand com er o f the micrograph, there is a cluster o f large lipid droplets. In the upper right hand comer, there is a large sinusoid. L6211. Liver. Hepatocyte. 10,200X. Approximately 90% o f a hepatocyte is observed along the bottom right hand portion o f the micrograph. There is a large area o f chatter over the cytoplasm o f this cell that makes interpretation somewhat difficult. The hepatocyte has a centrally located round nucleus. The cytosol o f this cell is filled with numerous mitochondria, RER, and patches o f SER. A few lipid droplets are also observed. Fifteen (15) peroxisomes are counted. Along the lower left hand edge, there is a large sinusoid containing a num ber o f erythrocytes. L6212. Liver. Hepatocyte. 7,920X. A somewhat oval hepatocyte is found in the center o f the micrograph. The nucleus is distinctly round with a small nucleolus. The cytosol is filled with num erous slightly swollen m itochondria and a few randomly scattered lipid droplets. The RER is abundant and often slightly dilated. Twenty-two (22) peroxisomes are counted. The plasma membrane o f the cell has numerous villous projections. Sinusoids on the upper left-hand side as well as the bottom o f the micrograph contain a number o f small erythrocytes. The bottom sinusoid also contains a leukocyte. Conclusions: SER is increased in 4/5 hepatocytes. Peroxisome average is 20.0 per hepatocyte. 3348 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: CrkCD8 (SD IGS BR A nim al N o.: C90724 Tissue: Liver- Periportal Treatm ent Group: 1 (Control) Block No(s).: C90724 1A Sex: M ale Photo/Negative N ofs).:L6213-L6217 Significant Lesions (check one): Yes X No Interpreting Pathologist (signature and date): M arch 26. 2001 Features: L6213. Liver. H epatocyte. 7,530X. An oval hepatocyte is found horizontally in the center o f the m icrograph. A som ew hat eccentrically located nucleus is com pressed on one side due to a large lipid droplet. The nucleus contains a sm all nucleolus. The cytosol o f this cell contains num erous sm all round m itochondria; som e contain crystalline inclusions. Random ly scattered are patches o f RER. Tw enty (20) peroxisom es are counted. A bove and below this cell, there are dilated sinusoids containing erythrocytes. Sim ilar hepatocytes adjacent to this cell contain sim ilar organelles. L6214. Liver. H epatocyte. 7,530X . A polygonal-shaped, centrally located hepatocyte contains two nuclei. One nucleus has a centrally located nucleolus. The cytosol o f this cell contains num erous m itochondria and RER. A few random ly scattered glycogen particles are also observed. Tw enty-seven (27) peroxisom es are counted. L6215. Liver. H epatocyte. 7,530X. In the center o f the m icrograph, there is a whole som ew hat oval hepatocyte. This cell has a large round nucleus w ith a central nucleolus. The cytoplasm o f the cell is filled w ith num erous m itochondria, and RER. Tw enty-nine (29) peroxisom es are counted. The plasm a m em brane often contains a few small villous projections. W ithin a sinusoid running vertically along the left side o f the m icrograph, there is a cluster o f erythrocytes and a plasm a cell. A faint knife m ark (artifact) is found along the right hand side o f the hepatocyte. L6216. Liver. H epatocyte. 7,920X. A pyram idal-shaped hepatocyte is found in the center o f the m icrograph. The nucleus is large and rounded w ith a single nucleolus. The cytosol contains a num ber o f m itochondria, RER and a few random ly scattered lysosom es. A sm all fragm ent o f electron dense m aterial (artifact) is found on the left side o f the cell. Fourteen (14) peroxisom es are counted. The cell m em brane is som ew hat sm ooth w ith a few random ly scattered villous projections. Below this cell, there is a hepatocyte that contains a large cluster o f lipid droplets. A bove the hepatocyte, a wide sinusoid is found containing erythrocytes. Conclusions: N orm al hepatocytes. Peroxisom e average is 22.5 per hepatocyte. THIS PAGE REVISED. COVANCE STUDY NO.:6329-212 3349 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study N o.: 6329-212 EM 01.38 Species/Strain: CrI:CD (SD) IGS BR A nim al No.: C90726 Tissue: Liver-Periportal Treatm ent Group: 1 (Control) B lock N o(s).: C 90726 1A Sex: M ale Photo/N egative N ofsi.:L6218-L6223 S ig n ific a n t L e sio n s (c h e c k one): _________ Y es X No M arch 28. 2001 Features: L6217. Liver. Hepatocyte, 7,920X. In the center o f the micrograph is a cuboidal-shaped hepatocyte. This cell has a nucleus with an irregular nuclear membrane. Within the cytoplasm, there are a number o f lipid droplets, numerous mitochondria, and abundant RER. Thirteen (13) peroxisomes are counted. Adjacent hepatocytes also have a large number o f lipid droplets. L6218. Liver. Hepatocyte. 7,530X. This is a duplicate o f the hepatocyte in micrograph L6217. L6219. Liver. Hepatocyte. 7,530X. A pyramidal-shaped hepatocyte is located in the middle o f the micrograph. The nucleus is distinctly rounded. The cytoplasm o f this cell contains numerous mitochondria, RER, and lipid droplets. Seventeen (17) peroxisomes are counted. The cell membrane o f an adjacent hepatocyte has a number o f villous projections; these villi extend into the large sinusoid at the bottom left hand comer of the micrograph. This sinusoid contains erythrocytes and a single leukocyte. L6220. Liver. Hepatocyte. 7,920X. A large cuboidal hepatocyte is found in the upper half o f the micrograph. The cell contains a large nucleus with an irregular nuclear membrane.A small nucleolus is observed in the nucleus. The cytosol o f this cell is filled with RER, mitochondria, some glycogen particles, and a small cluster o f lipid droplets. A small sinusoid in the lower right hand comer o f the micrograph contains a small leukocyte, as does the one in the upper right hand comer. Thirteen (13) peroxisomes are counted. L6221. Liver. Hepatocyte. 7,920X. A somewhat rounded hepatocyte is found in the middle o f the micrograph. The cell has a centrally located distinctly rounded nucleus. The cytoplasm is filled with a number of large lipid droplets, abundant mitochondria, and RER. Twenty (20) peroxisomes are counted. Bordering this hepatocyte are other hepatocytes with similar characteristics. In the lower left hand comer of the micrograph, there is a portion o f a leukocyte within a sinusoid. L6222. Liver. Hepatocyte. 7,530X. This is a duplicate o f the hepatocyte in L6220. L6223. Liver. Hepatocyte. 7,530X. A round hepatocyte is observed in the center o f the micrograph. The cell has two nuclear profiles, one is irregular-shaped due to a number o f variable-sized lipid droplets compressing the nuclear membrane; the other nucleus is smaller and somewhat rounded. The cytoplasm contains a number of small mitochondria and RER. A few lysosomes are also observed. Twelve (12) peroxisomes are counted. Along the lower right comer o f the micrograph in a sinusoid, there is a somewhat oval cell filled with large lipid droplets (Ito cell). Conclusions: Mild lipid accumulation. Peroxisome average is 15.0 per hepatocyte. THIS P A G E REVISED. C O V A N C E S T U D Y NO.:6329-212 3350 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 E M 01.38 Species/Strain: CrkCD (SDi IGS BR A n im a l N o.: C 9 0 7 2 8 ______ Tissue: Liver -Centrilobular Treatm ent Group: 1 (Control) B lock N o(s).: C90728 1A Sex: M ale______ Photo/N egative N o(s).:L6224-L6228 S ig n ific a n t L esio n s (c h e c k one): _________ Y es X No Interpreting Pathologist (signature and date): M arch 27, 2001 Features: L6224. Liver. Hepatocyte. 4,990X. This micrograph contains hepatocytes surrounded by two sinusoids; one containing an Ito cell (upper right) and a lymphocyte (lower left). Between these sinusoids, there are two hepatocytes side by side. The hepatocyte on the right contains a rounded nucleus with one nucleolus. The cytoplasm o f this cell is filled with mitochondria, RER, and some SER. Four lipid droplets are also observed. Eleven (11) peroxisomes are counted. L 6225. Liver. H epatocyte. 10,200X . A pproxim ately 90% o f an oval hepatocyte is observed in the m iddle o f the m icrograph. The cell is characterized by a distinctly round nucleus having a centrally located nucleolus. W ithin the cytosol, there are a num ber o f m itochondria, RER, and SER. T hirteen (13) peroxisom es are counted. The cell m em brane contains num erous villous projections. L 6226. Liver. H epatocyte. 7,920X . A round hepatocyte is located in the center o f the m icrograph. This cell contains a large round nucleus with a central nucleolus. The cytoplasm o f this hepatocyte contains a large num ber o f m itochondria, some RER, and a few lysosom es and lipid droplets. E ighteen (18) peroxisom es are counted. A n Ito cell, is found at the bottom o f the m icrograph and is characterized by cytoplasm filled w ith large lipid droplets. L 6227. Liver. H epatocyte. 10,200X. A som ew hat round hepatocyte is found in the center o f the m icrograph. The cell has an eccentrically located nucleus w ith finely granular chrom atin. W ithin the cytosol, there are a few patches o f RER, a m oderate num ber o f m itochondria, and abundant SER. A few small lysosom es and tw o som ew hat sm all lipid droplets are also observed. Sixteen (16) peroxisom es are counted. Two bile canaliculi are observed betw een this hepatocyte and the adjacent hepatocytes on the left o f the m icrograph. In the upper right hand com er o f the m icrograph, a sinusoid is observed containing two partial erythrocytes. L6228. Liver. Hepatocyte. 7,920X. A cuboidal shaped hepatocyte is found in the center o f the micrograph. The cell has a centrally located nucleus. The cytosol contains numerous small mitochondria, RER, SER, and a cluster o f small lysosomes. Small to moderate sized lipid droplets are also observed. Seventeen (17) peroxisomes are observed. Conclusions: Norm al hepatocytes. Peroxisome average is 15.0 per hepatocyte. 3351 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study N o.: 6329-212 EM 01.38 Species/Strain: C rkC P* tSD) IGS BR A nim al No.: C90904 Tissue: Liver - Centrilobular T re a tm e n t G ro u p :4 (M id-high IQOppm NEtFOSE) B lo ck N o (s).: C 9 0 9 0 4 1A Sex: M ale Photo/Negative N o(s).:L6229-L6233 S ig n ifican t L esio n s (c h e c k one): X Y es _______ N o Interpreting Pathologist (signature and date): _____ M arch 28. 2001 Features: L6229. Liver. Hepatocyte. 7,530X. Approximately 90% of a large round hepatocyte is observed in the center o f the micrograph. The nucleus is round with finely granular chromatin. The cytoplasm is filled with SER and minimal numbers o f mitochondria, and even less RER. In addition to the SER, there are a number of variable to medium sized lipid droplets. Seven (7) peroxisomes are counted. L6230. Liver. Hepatocyte. 4,990X. A large (compared to other hepatoeytes at this magnification) polygonal-shaped hepatocyte is found in the center o f the micrograph. The nucleus is small and round. The cytoplasm is extremely dark and filled with a large number o f small to medium sized lipid droplets. In addition to the lipid, there is an abundant amount o f SER and mitochondria. Peroxisomes not counted due to the dark staining of the cell. The adjoining hepatoeytes also are dark staining with abundant SER and large number o f lipid droplets. L6231. Liver. Hepatocyte. 4,990X. Similar to the hepatocyte in L6230, this cell is larger than normal (compared with comparable control hepatoeytes o f similar magnification). The cell contains two nuclei (plane o f section, assumed only one), one nucleus is large with a prominent nucleolus and a smaller nucleus with no nucleolus. The cytoplasm is distended with abundant SER, and numerous small to large lipid droplets. RER and mitochondria are also observed. Six (6) peroxisomes are counted. Bordering hepatoeytes are also filled with numerous lipid droplets. L6232. Liver. Hepatocyte. 4,990X. Another large oblong hepatocyte (compared to similar control hepatoeytes). This cell has two nuclei, one smaller than the other. The cytoplasm is filled with abundant SER, RER, and numerous small lipid droplets. One large electron dense lipid droplet is observed. Mitochondria and a few small stacks o f RER are observed as well. Nine (9) peroxisomes are counted. In the lower right hand com er o f the micrograph, there is another hepatocyte that also contains a large number o f lipid droplets. L6233. Liver. Hepatocyte. 4,990X. The cytoplasm o f a hepatocyte is found in the center o f the micrograph. The cytoplasm is extremely dilated due to a large number o f small, medium, and a few large lipid droplets. Peroxisomes not counted. _________________________________________ Conclusions: H epatocellular hypertrophy in 4/6 hepatoeytes. SER is increased as well as moderate lipid accumulation. Peroxisome average is 7.3 per hepatocyte._______________________ 3352 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study N o.: 6329-212 EM 01.38 S p ecies/S train: CrLCD (SD) IGS BR A n im al N o .: C 90908______ Tissue: Liver - Periportal T re a tm e n t G ro u p :4 (M id-high 1OOppm NEtFOSE) B lo ck N o (s).: C 9 0908 1A Sex : M ale______ Photo/Negative N ofsT:L6305-L6310 S ig n ifican t L esio n s (ch eck one): X Y es _______ N o In te rp re tin g P a th o lo g ist (sig n a tu re an d d ate): ( J > 4 '- ( t t l / f U T u t _____ M a rc h 28, 2001 Features: L6305. Liver. Hepatocyte. 10,200X. A somewhat round hepatocyte is located in the center of the micrograph. This cell contains a round nucleus with coarse chromatin. The cytoplasm is filled with abundant SER, and fewer mitochondria and RER. In addition to the SER, there are small to medium sized lipid droplets. Six (6) peroxisomes are counted. Surrounding this cell are wide sinusoids filled with clumped erythrocytes. L6306. Liver. Hepatocyte. 7,920X. An oval hepatocyte is found vertically positioned in the center of the micrograph. The cell has an eccentrically located nucleus with coarse chromatin. The cytoplasm contains numerous mitochondria, patches o f SER, and less but prominent RER. In addition, there are a few lipid droplets as well. Ten (10) peroxisomes are counted. L6307. Liver. Hepatocyte. 7,920X. A cuboidal-shaped hepatocyte is found obliquely positioned in the center of the micrograph. This cell has a somewhat small nucleus with coarse chromatin. The cytoplasm contains numerous mitochondria, RER, some SER, and a few lipid droplets and an even smaller number of lysosomes. Eleven (11) peroxisomes are counted. A clump o f electron dense debris is found along the edge o f the cell (artifact). In the left hand comer o f the micrograph, there is a clump of erythrocytes. A dilated bile canaliculus is found near the bottom on the left of the micrograph. L6308. Liver. Hepatocyte. 7,530X. A round hepatocyte is observed in the center o f the micrograph. This cell has two nuclei, one is slightly indented due to a large lipid droplet while the other is somewhat rounded. The cytoplasm contains a number o f mitochondria, SER, RER, and a few medium sized lipid droplets. Ten (10) peroxisomes are counted. Similarly, hepatocytes surrounding this cell also contain medium to large lipid droplets. A clump o f erythrocytes and lipid droplets are observed in a sinusoid in the bottom left comer of the micrograph. The dark staining o f the upper left and the lighter staining of the lower right o f the micrograph are considered staining artifacts. L6309. Liver. Hepatocyte. 7,920X. An oblong-shaped hepatocyte is found obliquely positioned in the center o f the micrograph. The cell contains a somewhat cuboidal-shaped nucleus with an irregular nuclear membrane. Numerous mitochondria, RER, and SER are found in the cytosol. In addition, numerous variable-sized, mostly medium, lipid droplets are also observed. Twelve (12) peroxisomes are counted. L6310. Liver. Hepatocyte. 7,920X. A slightly rounded, dark staining hepatocyte is observed to the left of the micrograph center. The cell is characterized by a somewhat rounded nucleus and cytoplasm with mitochondria, RER, SER, and a few small to medium lipid droplets and 4 lysosomes. Nine (9) peroxisomes are counted. The hepatocyte to the upper left of this cell contains a small number of lysosomes. To the right o f this cell, there are three elongated bundles of collagen. A number o f clumped erythrocytes are found to the right and below this cell. _____________________________________ _ Conclusions: SER is increased in 5/6 hepatocytes. Lipid accumulation appears increased as well. Peroxisome average is 9.1 per hepatocyte.___________ __________________________________________ _ 3353 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: Crl:CD8 (SPI IGS BR Animal No.: C90926 Tissue: Liver -Periportal Treatment Group:4 (Mid-high lOOppm NEtFOSE) Block No(s).: C90926 1A Sex: Male Photo/Negative No(s1.:L6234-L6239 Significant Lesions (check one): X Yes No Interpreting Pathologist (signature and date): U //X l March 28. 2001 Features: L6234. Liver. Hepatocyte. 7,530X. A somewhat oval hepatocyte is found in the center o f the micrograph. The nucleus is eccentrically located, somewhat rounded with an irregular undulating nuclear membrane. There is a single large nucleolus in the center o f the nucleus. The cytosol contains numerous mitochondria, RER, and a large patch of SER. There are a few, 5, lipid droplets and a few small lysosomes. Six (6) peroxisomes are counted. Surrounding this cell are sinusoids containing erythrocytes, and cellular debris. Part o f a hepatocyte with similar characteristics is observed in the lower left hand comer. L6235. Liver. Hepatocyte. 7,53OX. A vertically positioned, oval hepatocyte is found in the center o f the micrograph. On the right o f the cell, the plasma membrane is difficult to discern. The nucleus o f this cell is slightly round with an irregular nuclear membrane. The cytoplasm contains a dense accumulation o f SER along the top, and mitochondria and RER scattered randomly throughout the cell. A small cluster o f lysosomes is found at the bottom o f the cell. Seven (7) peroxisomes are counted. Surrounding this cell, the perisinusoidal space is fdled with cellular debris, and an erythrocyte. A binucleated hepatocyte is found along the bottom left with similar characteristics as the one described while another hepatocyte at the top left hand corner contains lysosomes in addition to mitochondria and RER. L6236 (7,530X) and L6237 (4,990X). Liver. Hepatocellular debris. M icrograph is uninterruptible due to less than optimal fixation that causes difficulty in determining individual cell borders. There is an increase in the amount o f residual bodies, presumed to be most likely lipofuscin deposits. L6238. Liver. Hepatocyte. 4,990X. An oblong-shaped hepatocyte is found in the center o f this micrograph. The central cell is characterized by an eccentrically located nucleus with a large round nucleolus. The cytosol contains abundant RER and mitochondria. Six (6) peroxisomes are counted. Above and to the right o f this cell, there are a number of large lipid droplets that appear to be within the sinusoids. Two somewhat large round electron dense residual bodies are found on the left side o f the micrograph and below the cell described. L6239. Liver. Hepatocyte. 4,990X. A portion o f a hepatocyte containing large lipid droplets. Based on the magnification, these droplets are extremely large. Conclusions: Due to suboptimal fixation, cell morphology was difficult to interpret. There was evidence o f an increase in lysosomes most likely lipofuscin pigment. Peroxisome average is 6.3 per hepatocyte.______ _ _ _ _ _ _______________________ --____________________________ _____ THIS PAGE REVISED. COVANCE STUDY NO.:6329-212 DATE: / / - / V - D l 3354 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: Crl:CD8 (SD IGS BR Animal No.: C90927_____ Tissue: Liver - Centrilobular Treatment Group :4 (Mid-high lOOppm NEtFOSE) Block No(s).: C90927 1A Sex: Male_____ Photo/Negative No(/s).:L6240-L6244 Significant Lesions (check one): X Yes ________ No Interpreting Pathologist (signature and date): J M J T , JKP March 28, 2001 Features: L6240. Liver. Hepatocyte. 7,920X. A cuboidal-shaped hepatocyte is found in the center of the micrograph. The cell has a very small nucleus (sectioning artifact). The cytoplasm contains a large amount of SER, RER, and mitochondria. A few lysosomes and lipid droplets are found randomly scattered within the cell. Fourteen (14) peroxisomes are counted. Other hepatocytes surrounding this cell have similar characteristics. L6241. Liver. Hepatocyte. 7,520X. O ff to the center right is approximately 90% o f a hepatocyte. The nucleus o f this cell is eccentrically located with a small nucleolus. The cytoplasm is filled with numerous small round mitochondria, RER, and SER. A few lipid droplets are also observed. Nine (9) peroxisomes are counted. A sinusoid with two erythrocytes, and a phagocytic cell (Kupffer cell) is found along the left side o f the micrograph. The other hepatocytes in the micrograph have similar characteristics as the cell described. L6242. Liver. Hepatocyte. 7,530X. A large oval hepatocyte is found encompassing almost all o f the micrograph. The nucleus is eccentrically located with finely granular chromatin. The cytoplasm is distended with numerous mitochondria, RER, and abundant SER. Twenty (20) peroxisom es are counted. A leukocyte and two erythrocytes are found in a sinusoid at the left hand side o f the micrograph. A circular deposit o f electron dense material (artifact) is found in right top comer. L6243. Liver. Hepatocyte. 7,530X. A round hepatocyte is found in the center o f the micrograph. This cell has a somewhat eccentrically located round nucleus. The cytosol contains abundant mitochondria, RER, and SER. A few lipid vacuoles are also observed. Ten (10) peroxisomes are counted. Hepatocytes surrounding this cell also have similar characteristics. L6244. Liver. Hepatocyte. 7,530X. An oval shaped hepatocyte is located vertically in the center of the micrograph. The cell has a large round nucleus with one prominent nucleolus. The cytoplasm contains a number of mitochondria, RER, and some SER randomly scattered between mitochondria and lipid droplets. Seven (7) peroxisomes are counted. The plasma membrane on the right side o f the cell is characterized by numerous microvillous projections. A clump o f erythrocytes is found in the upper left hand com er o f the micrograph.__________________________ Conclusions: SER is increased. One hepatocyte is hypertrophic. Peroxisome average is 12.0 per hepatocyte.____________________ _______________________________________ _ 3355 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: CrhCD (SD IGS BR Animal No.: C90928 Tissue: Liver - Periportal Treatment Group:4 (Mid-high lOOppm NEtFOSE) Block No(s).: C90928 1A Sex: Male Photo/Negative No('sT:L6245-L6250 Significant Lesions (check one): X Yes No Interpreting Pathologist (signature and date): March 28. 2001 Features: L6245. Liver. Hepatocyte. 7,530X. A somewhat larger hepatocyte is observed horizontally positioned in the middle o f the micrograph. The cell has a large round nucleus with a large nucleolus. The cytoplasm is filled with SER, mitochondria, RER, and a number o f small to medium sized lipid droplets. Eight (8) peroxisomes are counted. Bordering hepatocytes also have lipid droplets and increased SER. Tissue folds (electron dense lines over the entire micrograph) are artifacts. L6246. Liver. Hepatocyte. 7,530X. A rather large hepatocyte (encompassing almost the entire micrograph) is found in the center o f the micrograph. The cell has two nuclei, one small and one large (sectioning artifact). W ithin the cytoplasm, there is an abundance o f SER separating small mitochondria and individual cistemae o f RER. Lipid droplets are found scattered along the outer periphery o f the cell. Fifteen (15) peroxisomes are counted. There is an increase in the amount of electron dense cellular debris on the micrograph (artifact). L6247. Liver. Hepatocyte. 7,530X. In the center o f the micrograph, there is approximately 90% of a single hepatocyte observed. This cell has a large central nucleus with a large nucleolus. The cytoplasm is filled with SER, RER, and small round mitochondria. A cluster o f lipid is found on the right side o f this cell as well as in the hepatocyte below. Fifteen (15) peroxisomes are observed. Tissue folds and staining artifact is present over the entire micrograph. L6248. Liver. Hepatocyte. 7,920X. A slightly larger cell is found in the center o f the micrograph. The nucleus is small and centrally located. The cytoplasm is filled with loosely packed SER, RER, and widely space mitochondria. A few medium sized lipid droplets are also observed. Ten (10) peroxisomes are counted. Similar to the other micrograph, staining artifact is scattered over the entire micrograph. L6249. Liver. Hepatocyte. 7,920X. Approximately 90% o f a hepatocyte is found in the middle o f the micrograph. The nucleus o f this cell is distinctly round and large. The cytoplasm contains abundant SER, RER, and mitochondria. Seven (7) peroxisomes are counted. Bordering hepatocytes contain similar characteristics but with slightly more lipid. L6250. Liver. Hepatocyte. 12,100X. High magnification o f the cytoplasm o f a hepatocyte. Within the cytoplasm, there are a number of mitochondria, wide spaced cistemae o f RER, and SER. Staining artifact is also present on this micrograph. Peroxisomes not counted. Conclusions: SER is increased. Four out o f six hepatocytes are hypertrophic. Peroxisome average is 11.0 per h ep ato cy te.____________________________________________________________ THIS PAGE REVISED. COVANCE STUDY NO.:6329-212 9_____ DATE: / / - / f -O f 3356 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: Crl:CDs (SD) IGS BR Animal No.: C91127 Tissue: Liver - Centrilobular Treatment Group: 1 (Control) Block Nofsi.: C91127 Sex: Female Photo/Negative No(s).:L6251-L6256 Significant Lesions (check one): ________ Yes X No Interpreting Pathologist (signature and date): March 27, 2001 Features: L6251. Liver. Hepatocyte. 7,530X. In the middle o f the micrograph, positioned somewhat horizontally there is an oval hepatocyte. This cell has a large indented nucleus with an eccentric nucleolus. In the cytosol, there are two large lipid droplets, numerous mitochondria, and RER. SER, and a few small lysosomes are also found. Thirteen (13) peroxisomes are counted. Above the hepatocyte, there is a sinusoid lined by a prominent endothelial cell. Another hepatocyte in the upper left hand corner that contains a large lipid droplet while another hepatocyte on the bottom o f the micrograph also has a large lipid droplet. L6252. Liver. Hepatocyte. 7,530X. Approximately 90% o f one hepatocyte is found in the center o f the micrograph. This cell is characterized by an eccentrically large round nucleus with a prominent nucleolus. The cytosol contains numerous mitochondria, randomly scattered RER, and a few lipid droplets. Eleven (11) peroxisomes are counted. L6253. Liver. Hepatocyte. 7,530X. A small hepatocyte in the center o f the micrograph is slightly compressed on the right by a large lipid droplet. The central hepatocyte has an eccentrically located nucleus with a prominent nucleolus. Within the cytosol o f this cell, there is one large lipid droplet, loosely scattered mitochondria, and scant RER. Eleven (11) peroxisomes are counted. L6254. Liver. Hepatocyte. 7,530X. A polygonal hepatocyte is found to the left o f the micrograph center. The cell membrane is irregular with microvilli. The cell has a large distinctly round nucleus with a marginating nucleolus. The cytosol is filled with numerous round mitochondria, RER, and a medium sized lipid droplet. Below this cell, there are multiple large lipid droplets, one is found along the bottom right of the micrograph. Eight (8) peroxisomes are counted. L6255. Liver. Hepatocyte. 7,530X. A n oval hepatocyte is found vertically positioned in the center of the micrograph. This cell has a large somewhat rounded nucleus with a single nucleolus. The cytoplasm contains a number of mitochondria, abundant RER, and randomly scattered patches of SER with associated glycogen. Four lipid droplets are also observed. Seven (7) peroxisomes are counted. Surrounding this hepatocyte, there are other hepatocytes that contain large lipid droplets, especially prominent in the upper left hand comer. SER in the other hepatocytes appear dilated. A large prominent endothelial cell is found lining the sinusoid in the upper right hand portion o f the micrograph. L6256. Liver. Hepatocyte. 7,530X. A pyramidal-shaped hepatocyte is found in the center of the micrograph. The cell has two round nuclei, one has a large prominent nucleolus and the other has none (possibly plane o f section). The cytosol o f this cell contains numerous mitochondria, RER, and patches of loosely arranged glycogen. Four large lipid droplets are also observed. Fifteen (15) peroxisomes are counted. Conclusions: Normal hepatocytes. Mild lipid accumulation. Peroxisome average is 10.8 per hepatocyte. THIS P A G E REVISED. ' COVANCESTUDY C>9 DATE: 3357 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study N o.: 6329-212 EM 01.38 Species/Strain: Crl:CDs (SD) IGS BR A nim al No.: C91131 Tissue: Liver -Periportal Treatm ent Group: 1 (Control) Block No(s).: C91131 1A Sex: Fem ale Photo/N egative N o(s).:L6257-L6262 S ig n ifican t L esio n s (c h e c k one): _________ Y es Interpreting Pathologist (signature and date): X No r M arch 27. 2001 Features: L6257. Liver. Hepatocyte. 7,530X. A somewhat oval hepatocyte is found centered on the micrograph. The cell is characterized by a somewhat oval nucleus with a prominent marginating nucleolus and a cytoplasm filled with numerous mitochondria, RER, and a number o f large lipid droplets. Some of the lipid droplets contain cellular debris. A few randomly scattered lysosomes and glycogen particles are also observed. Ten (10) peroxisomes are counted. Surrounding this hepatocyte is a dilated sinusoid lined by prominent endothelial cells and containing a number of clumped erythrocytes and along the bottom of the micrograph, a perisinusoidal lipid-laden fat storing cell (Ito cell). L6258. Liver. Hepatocyte. 7,530X. A polygonal-shaped hepatocyte is found in the center of the micrograph. This cell has a small nucleus (sectioning artifact). The cytoplasm is distended with loosely arranged mitochondria, RER, and a large number o f lipid droplets. A number o f these droplets contain debris. Twelve (12) peroxisomes are counted. Surrounding this hepatocyte on all sides are dilated sinusoids with erythrocytes. A large perisinusoidal fat storing cell (Ito cell) is observed on the bottom of the micrograph. L6259. Liver. Hepatocyte - perisinusoidal space. 10,200X. Within a dilated sinusoid, there is a cell (presumed phagocytic, Kupffer cell) filled with large lysosomes and a few dilated RER cistemae. Also within this sinusoid are a few erythrocytes, and a perisinusoidal fat storing cell (Ito cell) located in the left bottom comer o f the micrograph. The microvillous border and the endothelial cell lining is quite prominent as is the space o f Disse. Portions o f hepatocytes found lining the outer edge o f this sinusoid are dark and filled with lipid droplets and mitochondria. No peroxisomes are counted. L6260. Liver. Hepatocyte. 7,530X. A centrally located binucleated hepatocyte is observed. The cytosol contains randomly scattered mitochondria, separated by SER, dilated cistemae of RER, and a few small lysosomes and medium sized lipid droplets. Ten (10) peroxisomes are counted. A prominent perisinusoidal fat storing cell (Ito cell) is found in the upper left hand comer o f the micrograph. Hepatocytes bordering the bottom half of the micrograph also contain lipid droplets. L626I. Liver. Hepatocyte. 7,530X. Two prominent oval hepatocytes are found side by side in the center of the micrograph. The hepatocyte on the left has a distinctly round somewhat eccentrically nucleus. The cytoplasm contains numerous stacks of RER, lesser SER, and a number of small round to elongate and slender mitochondria. Medium-sized lipid droplets are also observed. Fourteen (14) peroxisomes are counted. Located in the upper right hand comer o f the micrograph is a perisinusoidal fat storing cell that is somewhat compressing both hepatocytes. L6262. Liver. Hepatocyte. 7,530X. A somewhat cuboidal hepatocyte is found in the center o f the micrograph. This cell has a large round nucleus with a marginating nucleolus. The cytosol contains a number o f mitochondria, often clumped together and compressed due to large lipid droplets. A number of lipid droplets are observed. RER and SER as well as a few small lysosomes are also observed. Eight (8) peroxisomes are counted. [ Conclusions: Normal hepatocytes. Mild lipid accumulation. Peroxisome average is 10.8 per hepatocyte. THIS P A G E REVISED. C O V A N C E S T U D Y NO.:6329-212 3358 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: CrhCD tSD) IGS BR Animal No.: C91144_____ Tissue: Liver - Centrilobular Treatment Group: 1 (Control) Block No(s).: C91144 1A Sex: Female Photo/Negative No(s).:L6263-L6267 Significant Lesions (check one): ________ Yes X No Interpreting Pathologist (signature and date): March 28, 2001 Features: L6263. Liver. Hepatocyte. 7,530X. A round hepatocyte is found in the center o f the micrograph. The centrally located nucleus has an irregular nuclear membrane. The nucleus contains one large eccentrically located nucleolus. There are numerous mitochondria characterized by pale dilated cristae. Large cistemae o f RER are found as well as a few small randomly scattered lysosomes. A few lipid droplets are found along the periphery o f the cell. Fifteen (15) peroxisomes are counted. L6264. Liver. Hepatocyte. 7,530X. In the center o f the micrograph, there is a round hepatocyte with a somewhat rounded nucleus. The cytosol is filled with abundant RER and mitochondria. A few large lipid droplets and a few small lysosomes are also observed. Thirteen (13) peroxisomes are counted. This cell is bordered by other hepatocytes with similar characteristics. Along the bottom o f the micrograph, there is a sinusoid containing a leukocyte. The endothelial cell lining the right side o f this sinusoid is also observed. L6265. Liver. Hepatocyte. 7,920X. An oval hepatocyte is centered horizontally on the micrograph. This cell has an irregularly rounded nucleus with a somewhat marginating nucleolus. The cytoplasm o f the cell is filled with RER, mitochondria, a few variable-sized lipid droplets, and randomly scattered small patches o f SER. Thirteen (13) peroxisomes are counted. The cell membrane contains a number o f microvillous projections that extend into the surrounding sinusoids. L6266. Liver. Hepatocyte. 7,530X. In the center o f the micrograph is a somewhat pyramidal shaped hepatocyte. This hepatocyte has a centrally located round nucleus with a prominent eccentrically located nucleolus. The cytoplasm is filled with RER, SER, and numerous mitochondria. Lipid droplets, and a few small lysosomes are also observed. Twelve (12) peroxisomes are counted. A few tissue fissures are observed along the left side of the micrograph (sectioning artifact). L6267. Liver. Hepatocyte. 7,920X. A polygonal shaped hepatocyte is found in the center o f the micrograph. The cell has a large slightly cuboidal nucleus with a large round prominent nucleolus. Numerous mitochondria and stacks of RER are found within the cytosol. A few variable sized lipid droplets and a few lysosomes are also observed. Eleven (11) peroxisomes are counted. In the top right hand corner o f the micrograph, there are some clumped erythrocytes. In the lower right hand com er, there is a dilated sinusoid lined by prom inent endothelial cells. Conclusions: Norm al hepatocytes. Peroxisome average is 12.8 per hepatocyte. 3359 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: CrkCD fSDt IGS BR Animal No.: C91148 Tissue: Liver - Centrilobular Treatment Group: 1 (Control) Block No(s).: C91148 1A Sex: Female Photo/Negative No(s).:L6268-L6273 Significant Lesions (check one): Yes X No Interpreting Pathologist (signature and date): March 28. 2001 Features: L6268. Liver. Hepatocyte. 4,990X. A polygonal-shaped hepatocyte is found vertically located in the center o f the micrograph. This hepatocyte has a large oval nucleus with four prominent nucleoli. The cytoplasm contains abundant RER, some SER, and a few lipid droplets along the outer periphery o f the cell. Mitochondria are small and mild to moderately pleomorphic. Seven (7) peroxisomes are counted. The hepatocyte is surrounded by a dilated sinusoids filled with erythrocytes and some cellular debris. L6269. Liver. Hepatocyte. 7,920X. A somewhat rounded hepatocyte is found in the center o f the micrograph. The cell has a large oval nucleus with a prominent nucleolus. The cytosol contains numerous mitochondria, RER, less SER, and a few lipid droplets. Thirteen (13) peroxisomes are counted. A hepatocyte in the upper left hand com er o f the micrograph has similar characteristics as the central hepatocyte. L6270. Liver. Hepatocyte. 7,530X. A cuboidal-shaped hepatocyte is found in the center o f the micrograph. The cell has an eccentrically located nucleus with a prominent nucleolus. The cytoplasm is filled with num erous somewhat dilated cistem ae o f RER, a num ber o f pale staining mitochondria, and two large lipid droplets. Twelve (12) peroxisomes are counted. L6271. Liver. Hepatocyte - sinusoid. 7,920X. A perisinusoidal fat storing cell (Ito cell) is located in the lower h alf o f the micrograph. A large somewhat flat endothelial cell is observed in the center of the micrograph containing a large vacuole containing cellular debris and smaller, randomly scattered, vesicles. Hepatocytes located above and below this sinusoid are not described. Peroxisomes not counted. L6272. Liver. Hepatocyte. 7,530X. An oval hepatocyte in the center o f the micrograph is somewhat smaller than normal. The nucleus is round with an irregular undulating nuclear membrane. The cytoplasm is filled with large dilated endoplasmic reticulum. The micrograph is somewhat pale and the type o f endoplasmic reticulum is presumed smooth. Interwoven with the SER are numerous mitochondria with prominent dilated lamellar cristae. Peroxisomes not counted. L6273. Liver. Hepatocyte. 7,920X. A large polygonal-shaped hepatocyte is found in the center o f the micrograph. The nucleus o f this cell is round with a marginating nucleolus. The cytoplasm contains a number o f mitochondria separated by large cistemae o f RER and patches of SER. A few lipid droplets, some with cellular debris, are also observed. Fourteen (14) peroxisomes are counted. Clumps o f erythrocytes are found long the upper left and right hand borders o f the micrograph. _________________________ _____________________________________ Conclusions: Norm al hepatocytes. Peroxisome average is 11.5 per hepatocyte._________________ THIS PAGE REVISED. COVANCE STUDY NO.:6329-212 2 DATE:_______________ 3360 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: CrkCD (SD11GS BR Animal No.: C91159_____ Tissue: Liver - Periportal Treatment Group: 1 (Control! Block No(s).: C91159 1A Sex: Female Photo/Negative Nofs).:L6274-L6278 Significant Lesions (check one): ________ Yes X No Interpreting Pathologist (signature and date): March 28. 2001 Features: L6274. Liver. Hepatocyte. 7,530X. A polygonal-shaped hepatocyte is found in the center of the micrograph. The cell has a small round nucleus with an irregular nuclear membrane. The cytosol is distended with large numbers o f mitochondria, abundant RER, a lesser SER. Also observed are a number o f small to medium-sized lipid droplets. Eleven (11) peroxisomes are counted. A few small lysosomes are also found randomly scattered within the cytosol. Above this hepatocyte, there is a large sinusoid filled with erythrocytes and a small perisinusoidal fat storing cell (Ito cell) L6275. Liver. Hepatocyte. 7,920X. A polygonal-shaped hepatocyte is found in the center o f the micrograph surrounded on four sides by other hepatocytes with similar characteristics. A large sinusoid is found at the bottom o f the micrograph. Microvilli on the cell membrane o f the central hepatocyte are found projecting into the lumen o f the sinusoid. The central hepatocyte is characterized by a bean-shaped nucleus. The cytoplasm is filled with SER, RER, and numerous mitochondria. Ten (10) peroxisomes are counted. L6276. Liver. Hepatocyte. 7,920X. A somewhat rounded hepatocyte with a distinctly rounded nucleus is found in the center o f the micrograph. The nucleus has a large round nucleolus. Within the cytosol, there are a number of similar-sized mitochondria, RER, and SER. Ten (10) peroxisomes are counted. A large clump o f erythrocytes is found in the upper left hand comer o f the micrograph. L6277. Liver. Hepatocyte. 7,530X. Approximately 95% o f an oval cell is observed in the center o f the m icrograph. The nucleus is distinctly rounded and the cytoplasm is filled with mitochondria and RER. A few small patches of SER are also observed. Thirteen (13) peroxisomes are counted. Four other hepatocytes are found surrounding this hepatocyte along the bottom and right side o f the micrograph; these cells have similar characteristics as the cell described. L6278. Liver. Hepatocyte. 7,920X. A polygonal-shaped hepatocyte is observed in the center o f the micrograph. The nucleus is distinctly rounded with a prom inent nucleolus and a less prom inent area o f clum ped chromatin. The cytoplasm is filled with m itochondria separated by SER and RER. Eleven (11 ) peroxisomes are counted.________________________________________ C o n c l u s i o n s : Normal hepatocytes. Peroxisome average is 11.0 per hepatocyte. 3361 Covance 6329-212 3MT-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study N o.: 6329-212 EM 01.38 Species/Strain: Crl:CD (SD IGS BR Anim al N o.: C91324 Tissue: Liver - Centrilobular T re a tm e n t G ro u p :4 (M id-high lOOppm NEtFOSE) B lo c k N o (s).: C 9 1 3 2 4 1A Sex: Fem ale Photo/N egative N o(si.:L6279-L6283 Significant Lesions (check one): X Yes No In te rp re tin g P a th o lo g ist (sig n a tu re an d date): ._________________________M arch 28, 2000011 Features: L6279. Liver. Hepatocyte. 7,530X. An oval-shaped hepatocyte is observed in the center o f the micrograph. The cell has a distinctly round nucleus with two small prominent nucleoli. The cytoplasm contains numerous mitochondria, RER, and SER. Small to medium sized lipid droplets are also observed. Thirteen (13) peroxisomes are counted. The plasma membrane of the cell has numerous microvillous projections. Surrounding this cell, the sinusoids contain a large amount o f cellular debris. A hepatocyte in the left hand lower comer has similar morphologic characteristics as the cell described. L6280. Liver. Hepatocyte. 7,920X. A somewhat round hepatocyte is found in the middle of the micrograph. The cell has a large round nucleus with an irregular nuclear membrane. Within the nucleus, there are two prominent nucleoli. The cytoplasm is filled with streams o f RER, numerous mitochondria, and an increase in SER. A few lipid droplets are also observed; some have small myelin figures. Eight (8) peroxisomes are counted. The bordering hepatocytes have a similar increase in SER and a number o f lipid droplets. Along the right hand side o f the micrograph, there is a phagocytic cell containing electron dense material (lipofuscin). L6281. Liver. Hepatocyte. 7,920X. The micrograph is dark that makes interpretation difficult. A large binucleated hepatocyte is located along the left hand side o f the micrograph. The nuclei are round; one has a prominent nucleolus. The cytoplasm is filled with abundant SER, and less RER and mitochondria. Small and medium sized lipid droplets are also observed. Six (6) peroxisomes are counted. On the lower right hand side o f the micrograph, there is a portion o f another hepatocyte that contains three large lipid droplets. L6282. Liver. Hepatocyte. 4,990X. A cuboidal-shaped hepatocyte is located somewhat in the middle o f the micrograph. This cell has a small round nucleus and abundant cytoplasm. Within the cytoplasm, there is an increase in SER, RER, and mitochondria. A few small and medium sized lipid droplets are also observed. Seven (7) peroxisomes are counted. There are a number o f small lysosomes in the cytoplasm as well. Additionally, the hepatocyte in the left hand comer also contains small lysosomes. L6283. Liver. Hepatocyte. 7,530X. A pyramidal-shaped hepatocyte is found in the center o f the micrograph on top of another hepatocyte. The pyramidal cell has an eccentrically located, round, nucleus. The cytoplasm is filled with mitochondria, RER, and a few patches o f SER. A number o f medium and some small lipid droplets along with a few small lysosomes are also observed. The hepatocyte below this cell also contains small lipid droplets and an increase in SER. The sinusoid on the right side o f the micrograph contains three erythrocytes. Peroxisomes are not counted due to the darkness of the cytoplasm. Conclusions: SER is increased. Both lipid and lysosomal accumulation appear slightly increased as well. Peroxisome average is 8.5 per hepatocyte._____________ _______________________________________ ___ THIS PAGE REVISED. COVANCE STUDY NO.:6329-212 q ____ DATE: / / --/ S ' - O / 3362 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: CrkCD (SDl IGS BR Animal No.: C91332_____ Tissue: Liver - Periportal Treatment Group:4 (Mid-high 1OOppm NEtFOSEl Block No(s).: C91332 1A Sex: Female Photo/Negative No(s).:L6284-L6288 Significant Lesions (check one): X Y e s ________ No Interpreting Pathologist (signature and date): . --- O^ March 28. 2001 Features: L6284. Liver. Hepatocyte. 4,990X. The micrograph has a central sinusoid surrounded by five cuboidal shaped hepatocytes. These hepatocytes are the same shape and have the same morphologic characteristics. Three have a central round nucleus. All o f the cells have abundant mitochondria, RER, and an increase in SER. One hepatocyte has a large number o f electron dense lysosomes. Peroxisomes not counted. L6285. Liver. Hepatocyte. 7,530X. A slightly larger binucleated hepatocyte is found to the left o f the center o f the micrograph. The nuclei are round and o f the same size, both have nucleoli; one nucleus has a large nucleolus. The cytosol is characterized by an increase in SER, less RER, and numerous small similar sized mitochondria. A few lipid droplets are also observed. Seven (7) peroxisomes are counted. Along the bottom edge o f the cell, there is a small accumulation o f lysosomes. Similarly, the hepatocyte below and adjacent to this cell also contains some lysosomes. In the upper right hand edge o f the micrograph, there is a large sinusoid with one small erythrocyte. L6286. Liver. Hepatocyte. 4,990X. A somewhat oblong hepatocyte is observed in the center o f the micrograph surrounded by other hepatocytes o f similar size and morphologic characteristics. The central cell has a distinctly round nucleus with a prominent nucleolus. Within the cytosol, there are a number o f mitochondria, RER, some SER, and an increased number o f small to medium sized lipid droplets. Peroxisomes difficult to discern; therefore, not counted. L6287. Liver. Hepatocyte. 7,530X. A somewhat oval-shaped hepatocyte is observed in the center of the micrograph surrounded by similar hepatocytes. This cell has an oval to round nucleus with a central prominent nucleolus. The cytoplasm is packed with similar-sized mitochondria, some RER, and a few small lipid droplets. To the right side o f the cell, there are a few small lysosome granules. Nine (9) peroxisomes are counted. Hepatocytes above and below this cell have an increase in SER as well as minimal lipid accumulation. L6288. Liver. Hepatocyte. 7,920X. A cuboidal-shaped hepatocyte is found within the center of the micrograph. This cell has a slightly rounded nucleus with an irregular nuclear membrane. A m arginating nucleolus is found within the nucleus. The cytoplasm is characterized by abundant mitochondria, some RER, a few small patches o f SER and lipid droplets. Three of the lipid droplets have myelin figures. Ten (10) peroxisomes are counted.______________________________ Conclusions: Mild increase in SER. A few lysosomes observed as well. Peroxisome average is 8.7 per hepatocyte.______________________ ________________________________________________ 3363 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study No.: 6329-212 EM 01.38 Species/Strain: CrkCD (SD) IGS BR Animal N o.: C91335_____ Tissue: Liver - Periportal Treatment Group:4 (Mid-high lOOppm NEtFOSE) Block No(s).: C91335 1A Sex: Female Photo/Negative No(s).:L6289-L6293 Significant Lesions (check one): X Y e s ________ No Interpreting Pathologist (signature and date): M arch 28. 2001 Features: L6289. Liver. Hepatocyte. 10.200X. Approximately 90% o f a hepatocyte is found filling the whole micrograph. The cell has a large round nucleus with finely granular chromatin. The cytoplasm is filled with an increase in SER, RER, and a number o f small round mitochondria. There are a number o f small to medium sized lipid droplets also scattered throughout the cell. Twelve (12) peroxisomes are observed. A knife mark is found running thru the cell (artifact). L6290. Liver. Hepatocyte. 7,920X. A large cuboidal-shaped hepatocyte is found in the center o f the m icrograph. The cell has a large round nucleus with a large nucleolus. The cytoplasm is filled with numerous small mitochondria, RER, and SER. Lipid droplets are also observed. Fifteen (15) peroxisomes are counted. The bordering hepatocytes have similar characteristics especially an increase in SER. L6291. Liver. Hepatocyte. 7,530X. A slightly larger oval hepatocyte is observed in the middle o f the micrograph. The cell has a rounded nucleus and abundant cytoplasm. Mitochondria, RER, and an increase in SER are prominent features in the cytosol. There are a number o f randomly scattered small and medium sized lipid droplets. Thirteen (13) peroxisomes are counted. Bordering hepatocytes have similar increase in SER and lipid. L6292. Liver. Hepatocyte. 7,530X. A round hepatocyte is found in the center o f the micrograph. The cell has a round nucleus and a cytoplasm filled with medium size lipid droplets. Additionally, mitochondria, and RER, a few patches o f SER, and a small number o f lysosomes are also observed. Nine (9) peroxisomes are counted. Bordering this cell in the lower left hand corner o f the micrograph, there is another hepatocyte with also increased SER and some lysosomes. Above this cell, there is a wide sinusoid filled with serum plasma, erythrocytes, and a leukocyte. L6293. Liver. Hepatocyte. 7,530X. A slightly larger oblong hepatocyte is found in the center o f the micrograph. The cell has a large round nucleus. The cytosol is filled with numerous mitochondria, RER and SER. A small number o f lipid droplets are also observed. Thirteen (13) peroxisom es are counted. A knife mark thru the middle o f the cell is an artifact o f sectioning. Conclusions: SER is increased. Three hepatocytes are slightly larger than normal. Peroxisome average is 12.4 per hepatocyte. __________________________________________________ 3364 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study N o.: 6329-212 EM 01.38 Species/Strain: Crl:CD (SD IGS BR Anim al N o.: C91336 Tissue: Liver - Centrilobular T reatm en t G ro u p :4 (M id-high 1OOppm NBtFOSE) B lock N o(s).: C 9 1 3 3 6 1A Sex: Female Photo/N egative N o(s).:L6294-L6298 Significant Lesions (check one): X Yes No Interpreting Pathologist (signature and date): A A M arch 28. 2001 Features: L6294. Liver. Hepatocyte. 7,920X. A somewhat round hepatocyte is found in the center o f the micrograph. This cell has a large round nucleus. The cytoplasm contains numerous mitochondria, RER, and SER. In the left half of the cell, there is a large accumulation o f lipofuscin granules. Eleven (11) peroxisomes are counted. The surrounding hepatocytes have similar characteristics, especially an increase in SER. One hepatocyte, in the lower left hand comer o f the micrograph that contains lipoiuscin as well. There is a large sinusoid in the bottom right hand comer o f the micrograph that contains erythrocytes. L6295. Liver. Hepatocyte. 7,530X. A somewhat rounded hepatocyte is found in the middle o f the micrograph surrounded on three sides by other hepatocytes with similar characteristics. The central hepatocyte has a distinctly rounded eccentrically located nucleus that has two nucleoli; one is somewhat central while the other is marginating along the periphery o f the nucleus. The cytosol o f this cell is characterized by an increase in SER, some RER, and numerous mitochondria. Four lipid droplets are also observed. A few small lysosomes are found randomly scattered throughout the cell. Nine (9) peroxisomes are counted. Two other hepatocytes in the micrograph also contain some lipofuscin granules. A sinusoid along the bottom right of the micrograph contain a large amount o f cellular debris. L6296. Liver. Hepatocyte. 7,530X. A staining, pyramidal-shaped hepatocyte is found in the center of the micrograph. The cell has an eccentrically located nucleus that contains a large somewhat round nucleolus. The cytoplasm of this cell is characterized by numerous mitochondria, RER, and SER. Along the left half of the cell, there is a large accumulation o f lipofuscin. A few small to medium sized lipid droplets are also observed. Fourteen (14) peroxisomes are counted. Surrounding this hepatocytes are other hepatocytes with similar characteristics. A sinusoid in the upper left hand portion o f the micrograph contains a clump of erythrocytes. L6297. Liver. Hepatocyte. 7,530X. A slightly larger oblong hepatocyte (compared to other hepatocytes at same magnification), is found horizontally positioned in the center o f the micrograph. This cell has a somewhat small irregular nucleus. The cytoplasm is distended with an increase in SER, RER, numerous mitochondria, and a few randomly scattered lipid droplets. Some o f the lipid droplets have cellular debris or myelin figures. Twelve (12) peroxisomes are counted. Above this cell, there is another hepatocyte that appears slightly larger with an increase in SER also. L6298. Liver. Hepatocyte. 7,920X. A triangular-shaped hepatocyte is found in the center o f the micrograph. The cell has an extremely large nucleus. The cytoplasm is composed o f numerous mitochondria, RER, and randomly scattered patches of SER. A few lipid droplets, some with myelin figures, are also observed. Six (6) peroxisomes are counted. Bordering this hepatocyte on the right is a larger hepatocyte. A perisinusoidal fat storing cell (Ito cell) is found in the upper right hand comer o f the micrograph. In the lower left hand comer o f the micrograph, there is a sinusoid lined by a prominent endothelial cell. Conclusions: SER is increased. One hepatocyte is slightly hypertrophic Peroxisome average is 10.4 per hepatocyte.___________ _______________________________________________________________________ THIS PAGE REVISED. COVANCE STUDY NO.:6329-212 -y ? DATE: / A Q/ 3365 Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPH INTERPRETATION Study N o.: 6329-212 EM 01.38 Species/Strain: Crl:CDK(SD) IGS BR A nim al No.: C91341 Tissue: Liver - Centrilobular T re a tm e n t G ro u p :4 (M id-high IQQppm NEtFOSE) B lo ck N o (s).: C 91341 1A Sex: Fem ale Photo/Negative N o(s).:L6311-L6316 Significant Lesions (check one): X Yes No Interpreting Pathologist (signature and date): M arch 28. 2001 j Features: L6311. Liver. Hepatocyte. 7,53OX. An elongated oval obliquely positioned hepatocyte is observed in the middle of the micrograph. The cell has a large oval nucleus. The cytoplasm contains numerous small round mitochondria, RER, and patches o f SER. Ten (10) peroxisomes are counted. Surrounding this cell are dilated sinusoids that contain erythrocytes and cellular debris. In addition, to the left of this cell, there is another hepatocyte with no nucleus but with similar cytoplasmic characteristics. L6312. Liver. Hepatocyte. 7,920X. A pyramidal-shaped hepatocyte is observed in the center o f the micrograph positioned obliquely. The cell has a large somewhat oval nucleus that has a large prominent nucleolus. Within the cytosol o f this cell, there are three large lipid droplets. In addition to the lipid, there are a number of mitochondria, RER and SER. Five (5) peroxisomes are counted. The hepatocyte above this cell has even larger lipid droplets. L6313. Liver. Hepatocyte. 7,530X. An elongated oblong-shaped hepatocyte is observed in the center between two other hepatocytes with no nuclei. The central hepatocyte has a nucleus that is somewhat oval. The cytoplasm of this cell contains an increase in the amount o f SER along with mitochondria, RER, a few lipid droplets, and lysosomes. Seven (7) peroxisomes are counted. Adjacent to this cell on the left side o f the micrograph, there is a perisinusoidal fat storing cell (Ito cell). The sinusoid along the right side of the micrograph contains a finely granular material (serum plasma). L6314. Liver. Hepatocyte. 7,530X. An elongated binucleated cell is observed in the center o f the micrograph positioned somewhat obliquely. The cell nuclei are similar in shape and size except one has two nucleoli. The cytoplasm of this cell contains scattered SER, RER, and mitochondria. Fifteen (15) peroxisomes are counted. Along the outer periphery o f this cell, there are a number o f large lipid vacuoles. Two large perisinusoidal fat storing cells (Ito cells) are found; one to the left o f the cell, the other on the right bottom comer o f the cell. L6315. Liver. Hepatocyte. 7,530X. A cuboidal-shaped hepatocyte is found in the middle of the micrograph. The cell has a small eccentrically located nucleus. The cytoplasm contains abundant SER, less RER, and numerous mitochondria. Lipid droplets are also observed. Twelve (12) peroxisomes are counted. In the upper left half of the micrograph, a sinusoid is observed containing a number of erythrocytes. In the lower left hand comer, there is a sinusoid that contains a large leukocyte and two erythrocytes. L6316. Liver. Hepatocyte. 4,990X. Three hepatocytes are observed in the micrograph. The hepatocyte in the middle is characterized by indistinct cell membranes. This cell has a large round nucleus and a cytoplasm with SER, RER, and mitochondria. Peroxisomes are not counted due to the inability to determine cell boundaries._______________________________________________________________________ | Conclusions: SER is increased. Peroxisome average is 9.8 per hepatocyte. _________ THIS P A G E REVISED. C O V A N C E S T U D Y NO.:6329-212 ^ DATE: 3366 SECTION m Covance 6329-212 3M T-6316.1 TRANSMISSION ELECTRON MICROGRAPHS 3367 Covance 6329-212 3M T-6316.1 FIGURE LEGENDS Figure 1. Liver. Hepatocyte. This micrograph illustrates a normal hepatocyte. Note the numerous mitochondria and RER. Animal no. C90724, control male. Print magnification (PM) =7,530X. Bar = 1 micron Figure 2. Liver. Hepatocyte. . 7,920X. Another example of a normal hepatocyte. The cytoplasm of the cell is filled with RER, mitochondria, a few variable-sized lipid droplets, and randomly scattered small patches of SER. Animal no. C91144, control female. PM=7,920X. Bar = 1 micron. Figure 3. Liver. Hepatocyte. This hepatocyte is illustrating a large increase in SER. A few small to medium sized lipid droplets are also observed. Clumps of erythrocytes are found within surrounding sinusoids. Animal no. C90908, mid-high dose treated male. PM=10,200X. Bar = 1 micron. Figure 4. Liver. Hepatocyte. A large binucleated hepatocyte is found in the center of this micrograph. Note the large increase in SER and the numerous small to large lipid droplets. Bordering hepatocytes are also filled with numerous lipid droplets. Animal no. C90904, mid-high dosed treated male. PM=4,990X. Bar = 2 microns. Figure 5. Liver. Hepatocyte. A cuboidal-shaped hepatocytes is located somewhat in the middle of the micrograph. Within the cytoplasm, there is an increase in SER, RER, and mitochondria. In the bottom half of the cell, there is an accumulation of lysosomes. Additionally, the hepatocyte in the left hand comer also contains small lysosomes. The contents of the lysosomes are presumably lipofuscin pigments. Animal no. C91324, mid high dose treated female. PM = 4,990X. Bar = 2 microns. Figure 6. Liver. Hepatocyte. A somewhat round hepatocyte is found in the center of the micrograph. The cytoplasm contains numerous mitochondria, RER, and an abundance of SER. In the left half of the cell, there is a large accumulation of lysosome presumed to contain lipofuscin pigment. Surrounding hepatocytes have similar characteristics, especially an increase in SER. A large sinusoid in the bottom right hand comer of the micrograph contains some erythrocytes. Animal no. C91336, mid-high dose treated female. PM = 7,920X. Bar = 1 micron. 3368 Covance 6329-212 3M T-6316.1 3369 Covance 6329-212 3M T-6316.1 3370 Covance 6329-212 3M T-6316.1 3371 Covance 6329-212 3M T-6316.1 3372 V,,* Covance 6329-212 3M T-6316.1 3373 Covance 6329-212 3M T-6316.1 3374 Abbreviations BC E L LY M MVP N NU P RBC RER SER Covance 6329-212 3M T-6316.1 FIGURE LABELS FOR ULTRASTRUCTURAL STRUCTURES Structure Bile canaliculus Endothelial cell Lipid droplet Lysosome Mitochondria Microvillous projection Nucleus Nucleolus Peroxisomes Erythrocyte Rough endoplasmic reticulum Smooth endoplasmic reticulum 3375 SECTION IV Covance 6329-212 3M T-6316.1 QUALITY ASSURANCE STATEMENT 3376 Covance 6329-212 3M T-6316.1 PATHOLOGY ASSOCIATES A CHARLES RIVER C O M P A N Y QUALITY ASSURANCE STATEMENT The histopathology portion of this study has been inspected and audited by the Quality Assurance Unit of Pathology Associates International, as required by the Good Laboratory Practice regulations promulgated by the U.S. Food and Drug Administration. PAI has a functioning and responsive quality assurance unit which reports directly to management. The following is a record of inspections/audits and their resulting reports: Date of Inspection 03/14/01 03/31/01 11/15/01 Phase Inspected Date Findinas Reported to Management and Project Manaqer Darkroom Procedures Audit of Data and Draft Report Audit of Amended Final Report 03/14/01 03/31/01 11/15/01 mne deWard, B.S., RQAP-GLP Quality Assurance Officer // / Date Sponsor: Covance Laboratories Study Title: 104 Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Study Number: 6329-212 4915 D Prospectus Drive, Durham, North Carolina 27713 Phone: (919) 544-5257 Fax: (919) 544-3218 3377 C O V /H H > THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-212 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e IX o f X II Page 3378 o f4873 Covance 6329-212 3M T-6316.1 VOLUME IX OF XII CONTENTS Page A P P E N D I X 9 ...................................................................................................................................................................................... 3 3 8 0 C o v a n c e 6 3 2 9 - 2 2 8 ................................................................................................................................................................... 3 3 8 1 3379 APPENDIX 9 C ovance 6329-228 Covance 6329-212 3M T-6316.1 3380 C O V /H H > THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta Covance 6329-212 FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-228 Sponsor Study Identification: 3 M T -6 3 1 6 .1 Page 3381 o f4873 COMPLIANCE STATEMENT Covance 6329-212 Covance 6329-228 3M T-6316.1 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts A ll a sp e c ts o f th is stu d y w e re in a c c o rd a n c e w ith th e F o o d an d D ru g A d m in istra tio n G o o d L a b o ra to ry P ra c tic e R e g u la tio n s as set fo rth in T itle 21 o f th e U n ites S tates C o d e o f F e d e ral R e g u la tio n s, P a rt 58, issu e d D e c e m b e r 2 2 , 1978 (e ffe c tiv e Ju n e 2 0 , 1979), an d w ith an y a p p lic ab le a m e n d m e n ts. 3382 QUALITY ASSURANCE STATEMENT Covance 6329-212 Covance 6329-228 3M T-6316.1 T h is re p o rt, w ith th e e x ce p tio n o f A p p e n d ix 6, h a s b e e n re v ie w e d b y th e Q u ality A s su ra n c e U n it o f C o v a n c e L a b o ra to rie s In c ., in a c c o rd a n c e w ith th e F o o d a n d D ru g A d m in istra tio n (F D A ) G o o d L a b o ra to ry P ra c tic e R e g u la tio n s, 21 C F R 58. T h e fo llo w in g in sp e c tio n s w e re c o n d u c te d an d fin d in g s re p o rte d to th e stu d y d ire c to r an d stu d y d ire c to r m an a g em e n t. In sp e ctio n D ates F rom To 01 A p r 98 01 M ay 98 28 A pr 98 28 A pr 98 11 M a y 98 11 M a y 98 20 Jul 98 20 Jul 98 12 O ct9 8 12 O ct 98 04 Jan 99 04 Jan 99 03 M ay 99 03 M ay 99 02 Jun 99 02 Jun 99 26 Jul 99 26 Jul 99 09 N ov 99 08 N ov 99 26 A pr 00 26 A pr 00 11 M a y 0 0 16 M a y 0 0 25 A pr 00 24 M ay 00 25 A pr 00 01 Jun 00 25 Jul 00 11 A u g 0 0 11 A u g 0 0 11 A u g 0 0 11 A u g 0 0 11 A u g 0 0 11 A u g 0 0 11 A u g 0 0 30 A p r 01 01 M ay 01 13 N o v 01 13 N o v 01 13 N o v 01 13 N o v 01 P h ase P ro to c o l R e v ie w S a m p le C o lle c tio n B o d y W eig h ts A n im al O b serv a tio n s T est A rticle A d m in istra tio n F o o d C o n su m p tio n B o d y W eig h ts P ro to c o l A m e n d m e n t R e v ie w B o d y W eig h ts F o o d C o n su m p tio n P o stlife D a ta R e v ie w D a ta R e v ie w D a ta R e v ie w R e p o rt R e v ie w P ro to c o l A m e n d m e n t R e v ie w P ro to c o l A m e n d m e n t R e v ie w P ro to c o l A m e n d m e n t R e v ie w R e p o rt R e v ie w P ro to c o l A m e n d m e n t R e v ie w P ro to c o l A m e n d m e n t R e v ie w D a te R e p o rte d to S tu d y D ire c to r and S tu d y D ire c to r M a n a g e m e n t 01 A p r 98 28 A pr 98 11 M a y 98 20 Jul 98 12 O ct 98 04 Jan 99 03 M ay 99 04 Jun 99 26 Jul 99 08 N ov 99 26 A pr 00 19 M ay 00 24 M ay 00 01 Jun 00 11 A u g 0 0 11 A u g 0 0 11 A u g 0 0 11 A u g 0 0 03 M ay 01 13 N o v 01 13 N o v 01 /-i ~De. et / D ate 3383 STUDY IDENTIFICATION Covance 6329-212 Covance 6329-228 3M T-6316.1 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts T est M aterial: N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l S ponsor: 3M T o x ic o lo g y S erv ices B u ild in g 2 2 0 -2 E -0 2 , 3 M C e n te r S t. P a u l, M in n e s o ta 5 5 1 4 4 -1 0 0 0 S tu d y M o n ito r: A n d re w M . S e a ca t, P h D 3M 6 5 1 .5 7 5 .3 1 6 1 A lte rn a te S tu d y M o n ito r: Jo h n B u ten h o ff, P h D , D A B T 3M 6 5 1 .7 3 3 .1 9 6 2 S tu d y L o catio n : C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 S tu d y D ire cto r: P e te r J. T h o m fo rd , P h D C o v a n c e L a b o ra to rie s Inc. PO B ox 7545 M ad iso n , W isc o n sin 5 3 7 0 7 -7 5 4 5 6 0 8 .2 4 1 .7 2 0 7 S tu d y T im e ta b le : S tu d y In itia tio n D ate: In life S tart D ate: In life E n d D ate: S tu d y C o m p le tio n D ate: A p ril 1, 1 9 9 8 A p ril 6, 1998 A p ril 4, 2 0 0 0 D ecem b er 13, 2001 3384 KEY PERSONNEL Covance 6329-212 Covance 6329-228 3M T-6316.1 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts S tu d y D ire cto r: S tu d y T o x ic o lo g ist: S tu d y C o o rd in ato r: S u p erv iso r, S m a ll-A n im al T o x ico lo g y : S u p erv iso r, D o se F o rm u latio n : S u p erv iso r, C h e m istry /T o x S u p p o rt: A sso c ia te D ire cto r, L a b o ra to ry A n im al M ed icin e: C lin ic al P a th o lo g ist: S u p e rv iso r, C lin ic al P a th o lo g y : A n a to m ic P a th o lo g ist: S u p erv iso r, A n a to m ic P ath o lo g y : S u p erv iso r, A n a to m ic P ath o lo g y : S en io r S ta ff S cien tist: B io statistic ian : P rin c ip a l In v e stig a to r, C ell P ro life ra tio n P e te r J. T h o m fo rd , P h D T hom as E. R yan, BS C in d y L . P o w e rs, B S Jeni B arm an, A A S, A L A T , L A T D ix ie B u sh ee, B S , L A T G M ark H offm ann, B S , M B A D o n n a J. C le m o n s, D V M D ip lo m a te, A C L A M R o b e rt L . H a ll, D V M , P h D D ip lo m a te, A C V P (C lin ic al P a th o lo g y ) R o n a ld M a rk ev itch , B S , M T (A S C P ) Ja m es L . C arter, D V M , P h D D ip lo m a te, A C V P K im b erly W . D u rlan d , B S , H T L a u rie J. S c h u lle r, B A , L A T C y n th ia Y .C . L iu , M A A jit K . T h ak u r, P h D C a ro ly n M o y er, D V M D ip lo m a te, A C V P P a th o lo g y A sso c ia te s In tern a tio n a l 3385 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME IX OF XII CONTENTS Page A B S T R A C T ..........................................................................................................................................................................................3 3 9 0 P U R P O S E ...............................................................................................................................................................................................3 3 9 2 R E G U L A T O R Y C O M P L I A N C E ....................................................................................................................................3 3 9 2 T E S T M A T E R I A L , V E H I C L E , a n d C A R R I E R .............................................................................................. 3 3 9 2 T e s t M a t e r i a l ................................................................................................................................................................................. 3 3 9 2 V e h i c l e ................................................................................................................................................................................................ 3 3 9 3 C a r r i e r .................................................................................................................................................................................................. 3 3 9 3 R e s e r v e ( A r c h i v e ) S a m p l e s ..............................................................................................................................................3 3 9 3 D i s p o s i t i o n ...................................................................................................................................................................................... 3 3 9 3 T E S T A N I M A L S A N D H U S B A N D R Y ...................................................................................................................3 3 9 3 A n i m a l s .............................................................................................................................................................................................. 3 3 9 3 I d e n t i f i c a t i o n .................................................................................................................................................................................3 3 9 4 J u s t i f i c a t i o n .................................................................................................................................................................................... 3 3 9 4 H u s b a n d r y ........................................................................................................................................................................................3 3 9 4 A c c l i m a t i o n .................................................................................................................................................................................... 3 3 9 5 P R O C E D U R E S ................................................................................................................................................................................. 3 3 9 5 G r o u p D e s i g n a t i o n s a n d D o s e L e v e l s ...................................................................................................................3 3 9 5 D o s i n g P r o c e d u r e s ................................................................................................................................................................... 3 3 9 6 D o s e A n a l y s i s ............................................................................................................................................................................... 3 3 9 7 O b s e r v a t i o n o f A n i m a l s ...................................................................................................................................................... 3 3 9 8 C l i n i c a l P a t h o l o g y ....................................................................................................................................................................3 3 9 8 S e r u m A n a l y s e s .......................................................................................................................................................................... 3 3 9 9 A n a t o m i c P a t h o l o g y ...............................................................................................................................................................3 4 0 0 P r o l i f e r a t i o n C e l l N u c l e a r A n t i g e n E v a l u a t i o n .............................................................................................3 4 0 1 P a l m i t o y l - C o A O x i d a s e T i s s u e C o l l e c t i o n a n d A n a l y s e s ..................................................................3 4 0 1 S t a t i s t i c a l E v a l u a t i o n .............................................................................................................................................................3 4 0 3 S u r v i v a l a n d T u m o r A n a l y s i s .......................................................................................................................................3 4 0 3 R e c o r d R e t e n t i o n ....................................................................................................................................................................... 3 4 0 5 R E S U L T S A N D D I S C U S S I O N .......................................................................................................................................3 4 0 5 D o s e A n a l y s i s ............................................................................................................................................................................... 3 4 0 5 O b s e r v a t i o n o f A n i m a l s ...................................................................................................................................................... 3 4 0 6 C l i n i c a l P a t h o l o g y ....................................................................................................................................................................3 4 0 7 A n a t o m i c P a t h o l o g y ...............................................................................................................................................................3 4 0 7 P r o l i f e r a t i o n C e l l N u c l e a r A n t i g e n R e s u l t s .....................................................................................................3 4 0 7 C O N C L U S I O N ................................................................................................................................................................................. 3 4 0 8 3386 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME IX OF XII CONTENTS (Continued) Page S I G N A T U R E S ...................................................................................................................................................................................3 4 0 9 R E F E R E N C E S ...................................................................................................................................................................................3 4 1 0 P A T H O L O G Y R E P O R T ......................................................................................................................................................... 3 4 1 1 S T A T IS T IC A L R E P O R T O F S U R V IV A L , N O N N E O P L A S T IC , A N D N E O P L A S T I C L E S I O N S .................................................................................................................................................3 4 1 5 C O M M E N T S O N T H E D A T A .........................................................................................................................................3 4 2 5 C O D E S , A B B R E V I A T I O N S , A N D U N I T S .........................................................................................................3 4 2 7 G e n e r a l C o d e s a n d A b b r e v i a t i o n s .............................................................................................................................3 4 2 8 C o d e s f o r C l i n i c a l P a t h o l o g y ........................................................................................................................................ 3 4 2 9 A b b r e v i a t i o n s a n d U n i t s f o r C l i n i c a l H e m a t o l o g y .................................................................................... 3 4 3 2 A b b r e v i a t i o n s a n d U n i t s f o r C l i n i c a l C h e m i s t r y ......................................................................................... 3 4 3 3 A b b r e v i a t i o n s a n d U n i t s f o r C l i n i c a l U r i n a l y s i s ......................................................................................... 3 4 3 4 A b b r e v i a t i o n s a n d U n i t s f o r U r i n e C h e m i s t r y .............................................................................................. 3 4 3 4 C o d e s f o r A n a t o m i c P a t h o l o g y ................................................................................................................................... 3 4 3 5 F IG U R E S 1 A d j u s t e d S u r v i v a l D a t a ( % ) - M a l e s ............................................................................................................. 3 4 3 7 2 A d j u s t e d S u r v i v a l D a t a ( % ) - F e m a l e s .......................................................................................................3 4 3 8 3 M e a n B o d y W e i g h t D a t a ( g ) - M a l e s .......................................................................................................... 3 4 3 9 4 M e a n B o d y W e i g h t D a t a ( g ) - F e m a l e s ..................................................................................................... 3 4 4 0 5 M e a n F o o d C o n s u m p t i o n D a t a ( g ) - M a l e s ........................................................................................... 3 4 4 1 6 M e a n F o o d C o n s u m p t i o n D a t a ( g ) - F e m a l e s ......................................................................................3 4 4 2 TABLES 1 R e s u l t s o f H o m o g e n e i t y A n a l y s e s ( p p m ) ................................................................................................3 4 4 3 2 R e s u l t s o f S t a b i l i t y A n a l y s e s ( p p m ) ..............................................................................................................3 4 4 4 3 R e s u l t s o f D o s e P r e p a r a t i o n A n a l y s e s ( p p m ) ....................................................................................... 3 4 4 6 4 S u r v i v a l D a t a .......................................................................................................................................................................3 4 5 5 5 S u m m a r y o f C l i n i c a l O b s e r v a t i o n s .................................................................................................................3 4 6 4 6 S u m m a r y o f M a s s O b s e r v a t i o n s ........................................................................................................................3 4 7 1 7 S u m m a r y o f B o d y W e i g h t D a t a ( g ) ............................................................................................................... 3 4 8 0 8 S u m m a r y o f B o d y W e i g h t C h a n g e D a t a ( g ) ......................................................................................... 3 4 8 6 9 S u m m a r y o f F o o d C o n s u m p t i o n D a t a ( g ) ................................................................................................ 3 4 8 7 1 0 S u m m a r y o f T e s t M a t e r i a l C o n s u m p t i o n D a t a ( m g / k g / d a y ) .................................................. 3 4 9 3 1 1 S u m m a r y o f C l i n i c a l H e m a t o l o g y D a t a - W e e k 1 4 ........................................................................ 3 4 9 9 1 2 S u m m a r y o f C l i n i c a l H e m a t o l o g y D a t a - W e e k 2 7 ........................................................................ 3 5 0 3 1 3 S u m m a r y o f C l i n i c a l H e m a t o l o g y D a t a - W e e k 5 3 ........................................................................ 3 5 0 7 3387 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME IX OF XII CONTENTS (Continued) Page TABLES 1 4 S u m m a r y o f C l i n i c a l C h e m i s t r y D a t a - W e e k 4 ............................................................................... 3 5 1 1 1 5 S u m m a r y o f C l i n i c a l C h e m i s t r y D a t a - W e e k 1 4 ............................................................................. 3 5 1 3 1 6 S u m m a r y o f C l i n i c a l C h e m i s t r y D a t a - W e e k 2 7 ............................................................................. 3 5 1 9 1 7 S u m m a r y o f C l i n i c a l C h e m i s t r y D a t a - W e e k 5 3 ............................................................................. 3 5 2 3 1 8 S u m m a r y o f C l i n i c a l U r i n a l y s i s D a t a - W e e k 1 4 ............................................................................. 3 5 2 7 1 9 S u m m a r y o f C l i n i c a l U r i n a l y s i s D a t a - W e e k 2 7 ............................................................................. 3 5 2 9 2 0 S u m m a r y o f C l i n i c a l U r i n a l y s i s D a t a - W e e k 5 3 ..............................................................................3 5 3 1 2 1 S u m m a r y o f U r i n e C h e m i s t r y D a t a - W e e k 1 4 ................................................................................. 3 5 3 3 2 2 S u m m a r y o f U r i n e C h e m i s t r y D a t a - W e e k 2 7 ................................................................................ 3 5 3 5 2 3 S u m m a r y o f U r i n e C h e m i s t r y D a t a - W e e k 5 3 .................................................................................3 5 3 7 2 4 S u m m a r y o f O r g a n W e i g h t D a t a - W e e k 4 S a c r i f i c e .................................................................. 3 5 3 9 2 5 S u m m a r y o f O r g a n W e i g h t D a t a - W e e k 1 4 S a c r i f i c e ................................................................3 5 4 0 2 6 S u m m a r y o f O r g a n W e i g h t D a t a - W e e k 5 3 S a c r i f i c e ................................................................3 5 4 1 2 7 I n c i d e n c e o f M a c r o s c o p i c O b s e r v a t i o n s - W e e k 4 S a c r i f i c e ............................................... 3 5 5 6 2 8 I n c i d e n c e o f M a c r o s c o p i c O b s e r v a t i o n s - W e e k 1 4 S a c r i f i c e ........................................... 3 5 5 7 2 9 I n c i d e n c e o f M a c r o s c o p i c O b s e r v a t i o n s - W e e k 5 3 S a c r i f i c e ........................................... 3 5 5 8 3 0 I n c i d e n c e o f M a c r o s c o p i c O b s e r v a t i o n s - T e r m i n a l S a c r i f i c e ............................................. 3 5 6 9 3 1 I n c i d e n c e o f M a c r o s c o p i c O b s e r v a t i o n s - U n s c h e d u l e d D e a t h s ...................................... 3 5 8 3 3 2 I n c i d e n c e o f M a c r o s c o p i c O b s e r v a t i o n s - A l l A n i m a l s .............................................................. 3 6 0 1 3 3 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - W e e k 5 3 S a c r i f i c e ...............................................3 6 1 9 3 4 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - T e r m i n a l S a c r i f i c e ...............................................3 6 4 2 3 5 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - U n s c h e d u l e d D e a t h s ..........................................3 6 7 3 3 6 I n c i d e n c e o f M i c r o s c o p i c O b s e r v a t i o n s - A l l A n i m a l s .............................................................. 3 7 0 9 3 7 In c id e n ce o f S e v e rity o f S e lected M ic ro sc o p ic O b se rv a tio n s - W e e k 53 S a c r i f i c e ............................................................................................................................................................................... 3 7 4 9 38 In c id e n ce o f S e v e rity o f S e lected M ic ro sc o p ic O b se rv a tio n s - T erm in al S a c r i f i c e ............................................................................................................................................................................... 3 7 5 3 3 9 In c id e n ce o f S e v e rity o f S e lected M ic ro sc o p ic O b se rv a tio n s - U n sc h e d u le d D e a t h s ...................................................................................................................................................................................3 7 5 8 4 0 I n c i d e n c e o f S e v e r i t y o f S e l e c t e d M i c r o s c o p i c O b s e r v a t i o n s - A l l A n i m a l s ........ 3 7 6 3 A P P E N D I X 1 ...................................................................................................................................................................................... 3 7 6 8 P r o t o c o l D e v i a t i o n s ................................................................................................................................................................3 7 6 9 3388 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME X OF XII CONTENTS (Continued) Page A P P E N D I X 2 ...................................................................................................................................................................................... 3 7 7 4 I n d i v i d u a l A n i m a l F a t e D a t a .......................................................................................................................................... 3 7 7 5 I n d i v i d u a l C l i n i c a l O b s e r v a t i o n s ................................................................................................................................3 7 8 4 A P P E N D I X 3 ......................................................................................................................................................................................4 0 1 7 I n d i v i d u a l B o d y W e i g h t D a t a ( g ) .............................................................................................................................. 4 0 1 8 A P P E N D I X 4 ...................................................................................................................................................................................... 4 0 5 1 I n d i v i d u a l F o o d C o n s u m p t i o n D a t a ( g ) ...............................................................................................................4 0 5 2 I n d i v i d u a l T e s t M a t e r i a l C o n s u m p t i o n D a t a ( m g / k g / d a y ) ................................................................. 4 0 8 5 A P P E N D I X 5 ......................................................................................................................................................................................4 1 0 5 I n d i v i d u a l C l i n i c a l H e m a t o l o g y D a t a .................................................................................................................... 4 1 0 6 I n d i v i d u a l C l i n i c a l C h e m i s t r y D a t a ......................................................................................................................... 4 1 2 4 I n d i v i d u a l C l i n i c a l U r i n a l y s i s D a t a ......................................................................................................................... 4 1 4 0 I n d i v i d u a l U r i n e C h e m i s t r y D a t a .............................................................................................................................. 4 1 5 8 VOLUME XI OF XII A P P E N D I X 6 ......................................................................................................................................................................................4 1 6 6 I n d i v i d u a l A n a t o m i c P a t h o l o g y D a t a .................................................................................................................... 4 1 6 7 VOLUME XII OF XII A P P E N D I X 6 ( C o n t i n u e d ) .................................................................................................................................................... 4 5 0 8 I n d i v i d u a l A n a t o m i c P a t h o l o g y D a t a .................................................................................................................... 4 5 0 9 A P P E N D I X 7 ......................................................................................................................................................................................4 8 5 7 C e l l P r o l i f e r a t i o n R e p o r t ................................................................................................................................................ 4 8 5 8 3389 ABSTRACT Covance 6329-212 Covance 6329-228 3M T-6316.1 The purpose of this study was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE), when administered in the diet to rats for at least 104 weeks. This study was a supplementary study for Covance 6329-212. Male and female Crl:CD(SD)IGS BR rats were assigned to two groups (70 animals/sex/group). Group 8 received the control diet and Group 9 received diet containing 1 ppmN-EtFOSE.1. Food was provided a d libitum, except when animals were fasted. Water was provided a d libitum. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity. At least once each week, each animal was removed from its cage and examined for abnormalities and signs of toxicity. Body weight data were collected weekly through Week 17, once every 4 weeks thereafter, and at Week 105. Food consumption data were collected weekly for the first 16 weeks and once every 4 weeks thereafter. Blood and urine samples were collected for hematology, coagulation, clinical chemistry, urinalysis, and urine chemistry tests at specified time points. Blood was also collected to provide serum for possible future analysis. During Weeks 4, 14, 53, and 105, selected animals were anesthetized, weighed, exsanguinated, and necropsied (Weeks 53 and 105). Additional liver samples were also collected for hepatocellular proliferation (Week 14) and biochemical analyses from five animals/sex/group (Weeks 4 and 14). Liver sections from five animals/sex/group from Group 9 were stained with Oil Red "O" and examined microscopically. At necropsy, macroscopic observations were recorded, selected organs were weighed (Week 53), and selected tissues were collected and preserved. Animals that died on test or were sacrificed at unscheduled intervals were also necropsied, but organ weights were not recorded. Microscopic examinations were done on tissues from each animal in Groups 8, and any animals that died on test or were sacrificed at unscheduled intervals. Lesions, liver, lungs, kidneys, pancreas, thyroid, testes, and mammary glands (females) from each animal in Group 9 were examined microscopically. Test material consumption ranged from 0.029 to 0.108 mg/kg/day for Group 9. Thirty-nine control males and 37 control females and 30 males and 35 females given 1 ppm died on test or were sacrificed in a moribund condition. There were no significant 3390 Covance 6329-212 Covance 6329-228 3M T-6316.1 d iffe re n c e s in th e m o rta lity in b o th m a le s a n d fe m a le s. K a p la n -M e ie r e stim a te s in d ic a te th e re w a s n o tre a tm e n t-re la te d d e trim en tal e ffe c t o n th e su rv iv al o f e ith e r sex. M a le a n d fe m a le C rl:C D (S D )IG S B R ra ts w e re a d m in iste re d 0 o r 1 p p m o f (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l fo r at lea st 104 w eek s. T h ere w e re n o te st m a te ria l-re la te d effe c ts o n c lin ical o b se rv atio n s, b o d y w e ig h ts, fo o d c o n su m p tio n , c lin ica l p a th o lo g y re su lts, o rg a n w e ig h ts, m a c ro sc o p ic o r m ic ro sc o p ic fin d in g s. 3391 PURPOSE Covance 6329-212 Covance 6329-228 3M T-6316.1 T h e p u rp o se o f th is stu d y w a s to a sse ss th e c a rc in o g e n ic ity o f th e te s t m ate ria l, N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l (N -E tF O S E ), w h e n a d m in iste re d in th e d iet to rats fo r a t le a st 104 w e ek s. T h is stu d y w a s a su p p le m e n ta ry stu d y fo r C o v a n c e 6 3 2 9 -2 1 2 . REGULATORY COMPLIANCE A ll a sp e cts o f th is stu d y w e re c o n d u c te d in c o m p lian c e w ith th e F o o d an d D ru g A d m in istra tio n G o o d L a b o ra to ry P ra c tic e R e g u la tio n s as set fo rth in T itle 21 o f th e U n ite d S ta te s C o d e o f F e d e ra l R e g u la tio n s, P a rt 58, issu e d D e c e m b e r 2 2 , 1978 (e ffe c tiv e Ju n e 20, 1979), an d w ith an y a p p lic ab le am e n d m en ts. T h e p ro to c o l w a s re v ie w e d an d a p p ro v ed b y th e In stitu tio n al A n im al C a re an d U se C o m m itte e at C o v an ce. TEST MATERIAL, VEHICLE, AND CARRIER Test Material T h e te s t m ate ria l, T -6 3 1 6 (n a rro w ra n g e N -e th y l p e rflu o ro o c ta n e su lfo n a m id o eth an o l, N -E tF O S E ), a b le n d o f L o t N o s. 3 0 0 3 5 , 3 0 0 3 7 , a n d 3 0 0 3 9 , is a w a x y so lid a n d is 9 8 .1 % N -E tF O S E (w /w ) (se e P ro to c o l D e v ia tio n s fo r e x ce p tio n s). It w a s re c e iv e d at C o v an ce o n Ju n e 2 0 and Ju ly 21, 1997. T o av o id re p e a te d h e a tin g a n d su b lim a tio n , th e tw o sh ip m e n ts o f te s t m ate ria l w e re h e a te d , c o m b in e d , a n d p re d e te rm in e d q u a n titie s o f te s t m ate ria l w e re a liq u o te d in to p o ly eth y len e (L D P E ) co n tain ers. T h e te st m aterial w a s sto re d at ro o m tem p e ra tu re. In fo rm a tio n o n sy n th e sis m e th o d s, stab ility , c o m p o sitio n , o r o th e r c h a ra c te ristic s th a t d e fin e th e te s t m a te ria l w a s p ro v id e d b y th e S p o n s o r a n d is o n file w ith C o v a n c e . 3392 Covance 6329-212 Covance 6329-228 3M T-6316.1 Vehicle T h e v e h ic le w a s a c e to n e (S p e c tru m C h e m ic a l M fg . C o rp ., N e w B ru n sw ic k , N e w Je rse y a n d G a rd en a , C a lifo rn ia), L o t N o s. L W 0 0 4 4 (9 9 .9 6 % p u re ), L H 0 2 5 3 (9 9 .8 % p u re ), an d N S 0 2 3 1 (9 9 .8 % p u re). T h e sh ip m e n ts w e re re c e iv e d a t C o v a n c e o n A p ril 9 a n d Ju n e 2 3 , 1997, an d S e p tem b e r 8, 1998, resp ectiv ely . T h e v e h ic le w a s sto re d at ro o m tem p eratu re. In fo rm a tio n o n sy n th e sis m e th o d s, p u rity , c o m p o sitio n , o r o th e r c h a ra c te ristic s th a t d e fin e th e a c e to n e is o n file w ith th e m a n u fa c tu re r. Carrier C e rtifie d ro d e n t d iet (# 5 0 0 2 m eal, L a b D ie t b y P u rin a M ills, In c .) w a s u se d as su p p lied . T h e lo t n u m b e rs a re re c o rd e d in th e d a ta . T h e d ie t is ro u tin e ly a n a ly z e d b y th e m a n u fa c tu re r fo r n u tritio n al c o m p o n e n ts an d e n v iro n m e n ta l c o n ta m in a n ts. Reserve (Archive) Samples A re se rv e sa m p le (5 g ) o f th e te s t m ate ria l w a s ta k e n a n d sto re d at ro o m te m p e ra tu re . T h is sa m p le w ill b e tra n sfe rre d to th e S p o n so r a fte r c o m p le tio n o f th e in life p h ase. Disposition R e m a in in g te st m ate ria l w a s re tu rn e d to th e S p o n so r o n A u g u st 8, 20 0 0 . TEST ANIMALS AND HUSBANDRY Animals M a le an d fe m a le C rl:C D (S D )IG S B R ra ts w e re o b tain e d fro m th e R a le ig h , N o rth C a ro lin a , fa c ility o f C h a rle s R iv e r L a b o ra to rie s o n M a rc h 2 4 , 1998. A t in itia tio n o f tre a tm e n t, th e a n im a ls w e re 38 to 4 4 d a y s o ld ; th e m a le s w e ig h e d fro m 163 to 2 4 0 g, an d th e fe m a le s w e ig h e d fro m 126 to 179 g. 3393 Covance 6329-212 Covance 6329-228 3M T-6316.1 Identification Each animal was assigned a temporary number upon arrival. Before initiation of treatment, a microchip identification device was implanted into each animal. After randomization for placement on test, each animal was assigned a permanent number, and the microchip was coded with the permanent number. All data for an animal are recorded under these numbers. Justification Historically, rats have been used in safety evaluation studies and are recommended by appropriate regulatory agencies. Husbandry Animal Rooms 3045 and 335 were used for this study. Environmental controls for the animal room were set to maintain 18 to 26oC, a relative humidity of 30 to 70%, a 12-hour light/12-hour dark cycle, and a minimum of 10 room air changes/hour. Variations from these conditions are documented in the data and are considered to have had no effect on the outcome of the study. The animals were housed individually (except for the first 6 days of acclimation when animals were group-housed) in stainless-steel cages. Some animals were placed in polycarbonate cages during the study when health problems dictated. Certified rodent diet (#5002 meal, LabDiet by Purina Mills, Inc.) was provided a d libitum during acclimation. Water was provided a d libitum. Samples of the water are analyzed for specified microorganisms and environmental contaminants. The results are on file with Covance-Madison. There were no known contaminants in the diet or water at levels that would have interfered with this study. 3394 Covance 6329-212 Covance 6329-228 3M T-6316.1 A n im als n o t se lected fo r th e stu d y (15 m ale s an d 15 fe m a le s) w e re u se d fo r train in g p ro c e d u re s. T w o e x tra m a le s a n d o n e e x tra fe m a le w e re re c e iv e d a n d a lso u se d fo r train in g p ro ced u res. Acclimation O n e h u n d re d fifty -se v e n m ale s an d 156 fe m a le s w e re re c e iv e d o n M a rc h 2 4, 1998, and a c c lim a te d in A n im a l R o o m s 3 2 0 a n d 3 0 4 5 fo r 13 d a y s b e fo re in itia tio n o f tre a tm e n t (a n im a ls w e re tra n sfe rre d to A n im a l R o o m 3 0 4 5 o n A p ril 3, 1 9 98). In g e n era l, a n im a ls in th is sh ip m e n t a p p e a re d h ealth y . D u rin g a cc lim atio n , th e a n im a ls w e re e x a m in e d fo r a b n o rm a litie s in d ic a tiv e o f h e a lth p ro b le m s a n d b o d y w e ig h ts w e re re c o rd e d fo r all a n im a ls at ra n d o m iza tio n . PROCEDURES T h is s tu d y w a s c o n d u c te d in a c c o rd a n c e w ith th e P ro to c o l d a te d A p ril 1, 1 9 9 8 , a n d P r o to c o l A m e n d m e n t N o s . 1 t h r o u g h 6 . P r o to c o l d e v ia tio n s a r e in A p p e n d ix 1. Group Designations and Dose Levels T h e a n im a ls u se d fo r th is stu d y w e re e x a m in e d b y a la b o ra to ry a n im a l v e te rin a ria n o n A p ril 3, 1998, an d fo u n d to b e su itab le fo r stu d y c o n sid era tio n . S electio n o f a n im a ls fo r th e stu d y w a s b a se d o n a c c lim a tio n d a ta . A n im a ls w e re a ssig n e d to tre a tm e n t g ro u p s u sin g a c o m p u te riz e d b lo c k in g p ro c e d u re d esig n ed to ach iev e b o d y w e ig h t b a la n ce w ith re sp e c t to tre a tm e n t g ro u p . A t th e tim e o f ra n d o m iza tio n , th e w e ig h t v a ria tio n o f th e a n im a ls d id n o t ex ceed + 2 sta n d ard d e v ia tio n s o f th e m ea n b o d y w e ig h t fo r e ach sex. G ro u p m ea n b o d y w e ig h ts w e re a n aly ze d u sin g L e v e n e 's te s t fo r h o m o g e n e ity o f v a ria n c e at th e 5 .0 % p ro b a b ility lev e l a n d fo u n d to b e h o m o g en e o u s. A n im a ls w e re assig n e d to th e stu d y a c c o rd in g to th e fo llo w in g d esig n . 3395 Covance 6329-212 Covance 6329-228 3M T-6316.1 N o . o f A n im als D ie ta ry L ev e ls G roupa M a le F e m ale (p p m N -E tF O S E )b 8 ( C o n t r o l ) c,d,e 9 ( T r e a t e d ) d,e 70 70 70 70 0 1 a G ro u p s w e re d e sig n a te d as G ro u p s 8 a n d 9. T h is stu d y w a s p la c e d in th e sam e an im a l ro o m as C o v a n ce 6 3 2 9 -2 1 2 . b N -E tF O S E is 9 8 .1 % n -e th y l p e rflu o ro o c ta n e s u lfo n a m id o e th a n o l (N -E tF O S E ); d o se lev e ls are e x p ressed as p p m o f N -E tF O S E . c T h e c o n tro l a n im a ls re c e iv e d th e c o n tro l d ie t (b asal d ie t w ith a ce to n e ). d F iv e a n im a ls/se x /g ro u p w e re sa crificed d u rin g W e e k s 4 an d 14 fo r h e p a to c e llu la r p ro life ra tio n ra te m e a su re m e n ts an d b io ch e m ic al an aly se s (p a lm ito y l-C o A o x id atio n ). e T en a n im a ls/se x /g ro u p w e re d e sig n ated as in te rim sa c rifice a n im a ls an d w e re sa c rifice d a fte r at lea st 52 w e e k s o f trea tm e n t. Dosing Procedures Dose Preparation. D i e t a r y c o n c e n t r a t i o n s w e r e b a s e d o n t h e t e s t m a t e r i a l a s s u p p l i e d . D ie ts w e re p re p a re d at least o n ce ev ery 4 w eek s. Preparation of Diet for Group 8. T h e a p p r o p r i a t e a m o u n t o f c e r t i f i e d d i e t w a s w e i g h e d a n d m ix e d w ith a n a p p ro p ria te v o lu m e o f a ce to n e . Preparation of Stock and Base Diet. D i e t s w e r e p r e p a r e d i n c o n j u n c t i o n w i t h C o v a n ce 6 3 2 9 -2 1 2 . A sto c k p re p a ra tio n w a s m ad e u sin g th e re q u ire d a m o u n t o f te st m a te ria l d isso lv e d in th e re q u ire d v o lu m e o f a ce to n e . F o r th e 3 0 0 -p p m b a se d iet, a p re m ix c o n sistin g o f a p o rtio n o f th e c ertifie d d iet w ith th e re q u ire d a m o u n t o f th e sto ck p re p a ra tio n w a s m ix e d u sin g a H o b a rt m ix e r. T h e p re m ix w a s tra n s fe rre d to a m ix e r w ith ad d itio n al c ertifie d d iet an d m ix e d th o ro u g h ly . Diet for Group 9. F o r t h e 1 - p p m d i e t , a p r e m i x c o n s i s t i n g o f a p o r t i o n o f t h e c e r t i f i e d diet, th e re q u ire d a m o u n t o f th e p re p a re d b a se d iet, an d th e re q u ire d v o lu m e o f a ce to n e w a s m ix e d a n d th e n tra n s fe rre d to a m ix e r w ith a d d itio n a l c e rtifie d d ie t an d m ix e d th o ro u g h ly . S am p les fo r d o se a n aly ses w e re ta k e n d irec tly fro m th e m ix in g b o w l. 3396 Covance 6329-212 Covance 6329-228 3M T-6316.1 The diets were stored at room temperature in covered containers until dispensed into feeding jars. Method of Administration. Dietary admixture was used because the potential route of exposure in humans is oral. The dose preparations were administered a d libitum. Animals received the test diet for at least 104 weeks, as appropriate(see Protocol Deviations for exceptions). Retention Samples. Samples (approximately 100 g) were taken from each dose preparation, during the inlife phase, and stored at room temperature. Unless used for analyses, retention samples from the treated groups were discarded at least 1 month after completion of the inlife phase. Samples from the control diets prepared for Weeks 1 through 14 were shipped to the Sponsor. Dose Analysis Analyses for the concentration of test material in the dose preparations were done by Covance using an analytical method, MP-M312-MA, validated by Covance. Homogeneity was determined for the 1-ppm diet mixed for Week 1. One sample (approximately 100 g each) from the top, middle, and bottom of the diet was collected, divided into three subsamples for extraction and analysis, and analyzed for test material content. All samples were stored at room temperature until analyzed. To establish stability, two additional samples (approximately 100 g each) were taken from the 1-ppm diet mixed for Week 1. The middle homogeneity sample was analyzed on the day of mixing and served as the stability time zero sample. One sample was stored at room temperature for 21 days, then analyzed. The other sample was stored at room temperature for 32 days, then analyzed. Stability analyses were also conducted on another mix (June 5, 1998). The routine sample from that mix served as the time zero sample. Samples were stored at room temperature for 32 days or 46 days at room temperature, then analyzed. Samples (approximately 100 g each) were collected from all dose preparations and analyzed in duplicate. The homogeneity sample collected from the middle of the dose 3397 Covance 6329-212 Covance 6329-228 3M T-6316.1 preparation for Week 1 was used for dose confirmation results. All samples were stored at room temperature until analyzed. Observation of Animals Clinical Observations. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity (see Protocol Deviations for exceptions). Once prior to treatment and weekly thereafter, each animal was removed from its cage and examined; abnormal findings or an indication of normal were recorded. In addition, the time of onset, location, size, appearance, and progression of each grossly visible or palpable mass were recorded. Body Weights. Individual body weight data were recorded on Day -3 (randomization), weekly for Weeks 1 through 17 (see Protocol Deviations for exceptions), once every 4 weeks thereafter, and at Week 105. Food Consumption. Individual food consumption data were recorded weekly for Weeks 1 through 16 (see Protocol Deviations for exceptions) and once every 4 weeks thereafter (see Protocol Deviations for exceptions). Clinical Pathology Blood and urine samples were collected from 10 animals/sex/group during Weeks 14, 27, and 53. Blood films were made and held for possible future examination from animals sacrificed at unscheduled intervals and from animals at scheduled sacrifice after at least 104 weeks of treatment. Animals were fasted overnight, and urine was collected chilled for approximately 16 hours before blood sampling; water was provided a d libitum. Blood was collected from a jugular vein. The anticoagulant was potassium EDTA for the hematology tests. Samples for clinical chemistry and serum samples were collected without an anticoagulant. Animals were bled in random order. The following were evaluated. 3398 Covance 6329-212 Covance 6329-228 3M T-6316.1 Hematology re d b lo o d cell (e ry th ro c y te ) c o u n t h em o g lo b in h em ato crit m ea n c o rp u scu la r v o lu m e m ea n c o rp u scu la r h em o g lo b in m ea n c o rp u scu la r h em o g lo b in c o n ce n tra tio n p la te le t c o u n t w h ite b lo o d cell (le u k o c y te ) c o u n t d iffe re n tia l b lo o d cell c o u n t se g m en ted n e u tro p h il c o u n t ly m p h o c y te c o u n t m o n o cy te c o u n t e o sin o p h il c o u n t b a so p h il co u n t b lo o d cell m o rp h o lo g y R e tic u lo c y te sm e a rs w e re m ad e a n d h e ld fo r p o ssib le fu tu re e x am in atio n . g lu co se u re a n itro g e n c rea tin in e to ta l p ro tein alb u m in g lo b u lin ch o le stero l to ta l b iliru b in a p p e a ra n c e v o lu m e sp ecific g ra v ity pH p ro tein u ro b ilo g e n so d iu m p o tassiu m Clinical Chemistry a la n in e a m in o tra n sfe ra se g a m m a g lu ta m y ltra n sfe ra se a sp a rta te a m in o tran sfe ra se calciu m in o rg an ic p h o sp h o ru s so d iu m p o tassiu m ch lo rid e Urinalysis g lu co se k e to n e s b iliru b in b lo o d m ic ro sc o p ic e x a m in a tio n o f Urine Chemistry 1 6 -h o u r e x c re tio n of: so d iu m p o tassiu m Serum Analyses B lo o d (a p p ro x im ate ly 2 m L at th e W ee k s 4 an d 14 sa c rifice s an d a p p ro x im a te ly 3 m L at th e W e e k 53 an d 105 sa c rifice s) w a s c o llected . S am p les w e re c o lle cted fro m fiv e a n im a ls/se x /g ro u p d u rin g W e e k s 4 an d 14 (a n im als se lec te d fo r h e p ato ce llu la r 3399 Covance 6329-212 Covance 6329-228 3M T-6316.1 p ro life ra tio n a n d b io c h e m ic a l a n a ly se s); fiv e a n im a ls/se x /g ro u p (fro m a n im a ls se le c te d fo r in te rim sa c rifice ) a fte r at least 52 w e e k s o f trea tm e n t; an d 10 a n im a ls/se x /g ro u p at term in a l sacrifice. A n im a ls w e re fa ste d o v e rn ig h t an d b lo o d w a s c o lle cted w ith o u t an a n tic o a g u la n t fro m a ju g u la r v e in . B lo o d sam p les w e re a llo w e d to clo t at ro o m te m p e ra tu re , th e n c en trifu g e d . S e r u m s a m p l e s w e r e h a r v e s t e d a n d s t o r e d i n a f r e e z e r s e t t o m a i n t a i n - 6 0 t o - 8 0 oC . S a m p le s w e re sh ip p e d o n d ry ice to th e S p o n s o r fo r p o ssib le fu tu re a n a ly se s. Anatomic Pathology Necropsy. A n e c r o p s y w a s d o n e o n e a c h a n i m a l t h a t d i e d o r w a s s a c r i f i c e d a t a n u n sc h e d u le d in te rv al. A n im als w e re sa c rifice d w ith c a rb o n d io x id e , w e ig h e d , and e x sa n g u in a te d . D u rin g W e e k 4, fiv e a n im a ls/se x /g ro u p w e re fa ste d o v e rn ig h t, b le d fo r se ru m sa m p le s, a n e sth e tiz e d w ith c a rb o n d io x id e , w e ig h e d , a n d e x sa n g u in a te d . T h e liv e r w a s re m o v e d an d w e ig h e d . A d d itio n a l liv e r sam p le s w e re sto re d in a freezer, set to m ain tain - 6 0 t o - 8 0 oC , a n d s e n t t o t h e S p o n s o r f o r p o s s i b l e f u t u r e a n a l y s i s . D u rin g W e e k 14, fiv e a n im a ls/se x /g ro u p w e re fa ste d o v e rn ig h t, b le d fo r se ru m sa m p le s, a n e sth e tiz e d w ith c a rb o n d io x id e , w e ig h e d , a n d e x sa n g u in a te d . T h e liv e r w a s re m o v e d an d w e ig h ed . A d d itio n a l liv e r sa m p le s w e re sto re d in a freezer, set to m ain tain - 6 0 t o - 8 0 oC , a n d s h i p p e d t o t h e S p o n s o r f o r p o s s i b l e f u t u r e a n a l y s i s . A fte r at least 52 w e e k s o f trea tm e n t, 10 a n im a ls/se x /g ro u p w e re fa sted o v ern ig h t, b led fo r c lin ic a l p a th o lo g y (a ll a n im a ls) a n d se ru m sa m p le s (fiv e a n im a ls/se x /g ro u p ), a n e sth e tiz e d w ith c a rb o n d io x id e , w e ig h e d , e x sa n g u in a te d , an d n e c ro p sie d . A d d itio n a l liv e r sa m p le s ( f i v e a n i m a l s / s e x / g r o u p ) w e r e s t o r e d i n a f r e e z e r , s e t t o m a i n t a i n - 6 0 t o - 8 0 oC , a n d sh ip p e d to th e S p o n so r fo r p o ssib le fu tu re an aly sis. A fte r 104 w e e k s o f trea tm e n t, th e re m a in in g a n im a ls w e re fa sted o v e rn ig h t, b led fo r se ru m sa m p le s (1 0 a n im a ls/se x /g ro u p ), a n e sth e tiz e d w ith c a rb o n d io x id e , w e ig h e d , e x sa n g u in a te d , a n d n e c ro p sie d . L iv e r sa m p le s fro m 10 a n im a ls /s e x /g ro u p w e re sto re d in a f r e e z e r , s e t t o m a i n t a i n - 6 0 t o - 8 0 oC , a n d s h i p p e d t o t h e S p o n s o r f o r p o s s i b l e f u t u r e an aly sis. 3400 Covance 6329-212 Covance 6329-228 3M T-6316.1 T h e n e c ro p sy in clu d e d a m ac ro sc o p ic e x a m in a tio n o f th e e x tern al fe a tu re s o f th e carcass; e x tern al b o d y o rific e s; th e a b d o m in a l, th o ra c ic , an d c ran ial c a v itie s; o rg a n s; an d tissu e s. Proliferation Cell Nuclear Antigen Evaluation A t th e W e e k 14 co llectio n , re p re se n ta tiv e sa m p le s o f left late ra l lo b e o f th e liv e r an d m a c ro sc o p ic lesio n s o f th e liv e r w e re c o lle cted an d p re se rv e d in z in c fo rm alin . A fte r fix atio n , each sam p le o f th e liv e r w a s e m b e d d ed in p araffin , an d th e p a ra ffin b lo c k s w e re sh ip p e d to P a th o lo g y A sso c ia te s In te rn a tio n a l. P ro life ra tio n cell n u c le a r a n tig e n (P C N A ) e v a lu a tio n w e re d o n e o n th e sam p les. In a d d itio n , se c tio n s o f th e left late ra l lo b e o f th e liv e r w e re sta in e d w ith h e m a to x y lin a n d e o sin a n d e x a m in e d m ic ro sc o p ic a lly . Palmitoyl-CoA Oxidase Tissue Collection and Analyses A t th e W e e k 4 a n d 14 sa c rifice s, sa m p le (a p p ro x im a te ly 5 0 0 m g ) o f th e rig h t late ra l lo b e o f th e liv e r w a s also c o lle cted fro m e ac h an im a l an d fla sh -fro z e n in liq u id n itro g e n (see P ro to c o l D e v ia tio n s fo r e x c e p tio n s). T h e liv e r tissu e w a s sto re d in a freezer, set to m a i n t a i n - 6 0 t o - 8 0 oC , u n t i l a n a l y z e d f o r p a l m i t o y l - C o A o x i d a s e a c t i v i t y . Organ Weights. A t t h e i n t e r i m s a c r i f i c e d u r i n g W e e k 5 3 , t h e f o l l o w i n g o r g a n s w e r e w e ig h e d (see P ro to c o l D e v ia tio n s fo r e x c e p tio n s); p a ire d o rg a n s w e re w e ig h e d sep arately . ad ren al (2) b ra in k id n ey (2) liv e r lu n g o v ary (2) sp leen te stis (2) th y ro id (2 ) w ith p a rath y ro id u te ru s w ith c erv ix O rg a n -to -b o d y w e ig h t p e rc e n ta g e s a n d o rg a n -to -b ra in w e ig h t ra tio s w e re c alcu la te d . Tissue Preservation. T h e f o l l o w i n g t i s s u e s w e r e c o l l e c t e d a n d p r e s e r v e d i n 1 0% n e u tra l-b u ffe re d fo rm a lin (se e P ro to c o l D e v ia tio n s fo r ex ce p tio n s). 3401 adrenal (2) b ra in cecum c erv ix co lo n duodenum e p id id y m is (2) esophagus ey e (2) fe m u r w ith b o n e m a rro w (articu lar su rface o f th e d istal en d ) H a rd e ria n g lan d heart ileu m jeju n u m k id n ey (2) lesio n s liv e r lu n g w ith m ain stem b ro n ch i ly m p h n o d e (m esen teric) m a m m a ry g la n d (fem a le s o n ly ) o v ary (2) Covance 6329-212 Covance 6329-228 3M T-6316.1 p a n c re a s p itu itary p ro sta te re ctu m saliv ary g la n d [m an d ib u lar (2)] sciatic n erv e se m in a l v e sic le (2 ) sk e letal m u sc le (th ig h ) sk in sp in al c o rd (cerv ical, th o rac ic , an d lu m b ar) sp leen stern u m w ith b o n e m arro w sto m ach te stis (2) th y m u s th y ro id (2 ) w ith p a rath y ro id trach ea u rin ary b lad d er u teru s v a g in a B o n e m a rro w sm ears fro m th e fe m u r o f e ac h an im a l at th e W e e k 53 an d term in al sa c rific e s w e re p re p a re d , sta in e d w ith W rig h t's stain , a n d re ta in e d fo r p o ss ib le e x am in atio n . Histopathology. T i s s u e s ( a s a p p r o p r i a t e ) w e r e e m b e d d e d i n p a r a f f i n , s e c t i o n e d , s t a i n e d w ith h e m a to x y lin an d eo sin , an d e x am in ed m ic ro sc o p ic a lly (se e P ro to c o l D e v ia tio n s fo r e x c e p tio n s) fro m e a c h a n im a l in G ro u p 8 sa c rifice d at th e te rm in a l sa c rific e an d an y a n im a ls th a t d ied o n te s t o r w e re sacrificed at u n sc h e d u le d in te rv als. L e sio n s, liv e r, lu n g s, k id n e y s, p a n c re a s, th y ro id , te ste s, a n d m a m m a ry g la n d s (fe m a le s) fro m e ac h an im a l in G ro u p 9 w e re e m b e d d ed in p a rafilm , sectio n ed , stain ed w ith h e m a to x y lin an d eo sin , an d e x am in ed m ic ro sc o p ic a lly . P a ra th y ro id s w e re p ro c e sse d w ith th e th y ro id s b u t w e re n o t e x am in ed . A p a th o lo g y p e e r re v ie w w a s c o n d u c te d b y a p a th o lo g ist d e sig n ated b y th e sp o n so r. R e su lts are re p o rte d in C o v a n ce 6 3 2 9 -2 1 2 . 3402 Covance 6329-212 Covance 6329-228 3M T-6316.1 L iv e r se c tio n s fro m fiv e a n im a ls/se x /g ro u p fro m G ro u p 9 w e re sta in e d w ith O il R e d " O " an d e x am in ed m ic ro sc o p ica lly . Statistical Evaluation L e v e n e 's te s t (L ev en e, 1 9 6 0 ) w a s d o n e to te st fo r v a ria n c e h o m o g en e ity . In th e case o f h e te ro g e n e ity o f v a ria n c e a t p < 0 .0 5 , tra n s fo rm a tio n s w e re u se d to sta b iliz e th e v a ria n c e . C o m p a riso n te s ts to o k v a ria n ce h e te ro g e n e ity in to co n sid eratio n . O n e -w a y a n a ly sis o f v a ria n c e [A N O V A (W in e r, 1 9 7 1 )] w a s u se d to a n a ly z e in itial b o d y w e ig h ts, b o d y w e ig h t ch an g es, fo o d c o n su m p tio n , c o n tin u o u s clin ical p a th o lo g y v alu es, a n d o rg a n w e ig h t d ata. A N O V A w a s d o n e o n th e h o m o g e n e o u s o r tra n s fo rm e d d a ta . I f th e A N O V A w a s sig n ific a n t, stu d e n t's t-te st w a s u se d fo r p a irw ise c o m p a riso n s b e tw e e n tre a te d an d c o n tro l g ro u p s. G ro u p c o m p a riso n s (G ro u p 9 v e rsu s G ro u p 8) w e re e v a lu a te d a t th e 5 .0 % , tw o -ta ile d p ro b a b ility lev el. O n ly d a ta c o lle c te d o n o r a fte r th e first d a y o f tre a tm e n t w e re a n aly ze d sta tistica lly . Survival and Tumor Analysis S u rv iv a l w a s a n a ly z e d u sin g life ta b le te c h n iq u e s c o n sistin g o f K a p la n -M e ie r p ro d u c t lim it e stim ates, C o x -T a ro n e b in ary re g re ssio n , an d G e h a n -B re slo w n o n p a ra m e tric m e th o d s (T h o m as, B re slo w , an d G art, 1977). C o x -T aro n e m eth o d s are w e ig h te d h e av ier to w a rd s late in cid e n ce s an d G e h a n -B re slo w m e th o d s are w e ig h te d to w a rd s early in c id e n c e s d u e to trea tm e n t. A s a resu lt, th e y are b o th v a lu a b le to o ls fo r th e in cid e n ce d a ta w ith o n se t tim e s. W e e k 105 w a s tre a te d as th e e n d o f th e stu d y (E O S ) in th e N C I p a ck a g e fo r b o th sexes. T h e W ee k s 4, 14 an d 53 in te rim sa c rifice s a n im a ls an d W e e k 105 term in a l sa c rifice a n im a ls w e re c e n so re d in th e an aly sis. C o n tin u ity c o rre c te d tw o -sid e d tail p ro b a b ility w a s e v a lu a te d at th e 5 % sig n ific a n ce lev el fo r c o m p a rin g th e C o n tro l vs. T rea te d g ro u p . Nonneoplastic Lesions. N o n n e o p l a s t i c l e s i o n s w e r e c h o s e n f o r s t a t i s t i c a l a n a l y s e s i f t h e in c id e n c e ra te in th e tre a te d g ro u p w a s in cre ased o r d e cre ased b y at lea st tw o o c cu rre n ce s 3403 Covance 6329-212 Covance 6329-228 3M T-6316.1 o v e r th e c o n tro l g ro u p . T h ese w e re a n aly ze d b y o n e -sid e d F ish e r-Irw in e x ac t te s t at 5% sig n ific a n ce lev el (T h ak u r, B erry , an d M ie lk e , 1985). Neoplastic Lesions. N e o p l a s t i c l e s i o n s w e r e c h o s e n f o r s t a t i s t i c a l a n a l y s e s i f t h e in c id e n c e ra te in th e tre a te d g ro u p w a s in cre ased o r d e cre ased b y at lea st tw o o c cu rre n ce s o v e r th e c o n tro l. T h e in c id e n ta l tu m o rs (i.e ., tu m o rs th a t w e re n o t a ssig n e d to b e th e c a u se o f d e a th o f th e an im a ls b y th e stu d y p a th o lo g ist) w e re an aly ze d b y lo g istic re g re ssio n o f tu m o r p re v alen c e (D in se an d L ag ak o s, 1983). In th is a p p ro ach , tu m o r p re v alen c e w a s m o d e le d as a lo g istic fu n c tio n o f d o se a n d tim e. B o th lin e a r a n d q u a d ra tic te rm s in tim e w e re in c o rp o ra te d in itially , a n d th e q u a d ra tic te rm w a s e lim in a te d if th e re w a s la c k o f c o n v erg e n ce . F u rth e r, in th e c a se s w h e re th e re w a s la c k o f c o n v e rg e n c e fo r th e a sy m p to tic te st o f th e lo g istic re g re ssio n m eth o d , th e e x ac t p ro b a b ility o f th e te st w a s o b tain e d b y u sin g L o g X a c t-T u rb o (1 9 9 3 ). T h e fa ta l a n d p a lp a b le (su p e rfic ia l) tu m o rs w e re a n a ly z e d b y C o x -T a ro n e b in a ry re g re ss io n m e th o d as in th e c ase o f su rv iv a l, u sin g th e d e a th tim e o r th e first p a lp a tio n tim e ( if a p p lic a b le ) as th e tu m o r o n se t tim e. T h e b e n ig n an d m alig n a n t n e o p la stic in cid e n ce s w e re e v a lu a te d sep arately , as w e ll as co m b in ed , w h e re a p p ro p ria te . T h e c riteria fo r c o m b in a tio n w e re b a se d o n th e w o rk o f M c C o n n e ll e t al. (1 9 8 6 ). T h e in c id e n c e s o f m u ltip le - o r g a n fin d in g s s u c h a s fib ro m a , fib ro sa rc o m a, leio m y o m a , leio m y o sa rco m a, ly m p h o m a , an d e n d o m e tria l stro m al p o ly p w e re c o u n te d b y a n im a l, n o t b y tissu e ty p e. T h e y w e re e v a lu a te d sta tistic a lly if th e y m e t th e se lec tio n c rite rio n fo r th e an aly sis. T h e sta tistical re su lts fo r th e se c ases m ay b e b ia s e d b e c a u s e n o t all th e a n im a ls w e re e x a m in e d fo r e v e ry tis su e . O n e -s id e d ta il p ro b a b ilitie s fo r g ro u p c o m p a riso n w a s e v a lu a te d at th e 5 % sig n ific a n c e lev el. In th e n o n n e o p la stic an d n e o p la stic lesio n s, early sa c rifice d a n im a ls d u rin g o r b e fo re W e e k 14 in b o th g ro u p s w e re e x clu d ed fro m th e d e n o m in a to rs. O rd in al d o se lev els 0, an d 1 c o rre sp o n d in g to G ro u p s 8 (C o n tro l), an d 9 (T rea te d ) w e re u se d in th e ab o v e s ta tistic a l a n a ly sis. C o n tin u ity c o rre c tio n w a s d o n e fo r all th e a s y m p to tic te s ts . T h e fo llo w in g n o tatio n s are u se d th ro u g h o u t th is rep o rt. += -= E= E ffe c t in th e in cre ased d irec tio n E ffe c t in th e d e c re a se d d irec tio n E x act p erm u tatio n test 3404 Covance 6329-212 Covance 6329-228 3M T-6316.1 Record Retention T h e ra w d a ta , d o c u m e n ta tio n , re c o rd s, sp e c im e n s, p ro to c o l, a n d fin al re p o rt g e n e ra te d as a re su lt o f th is stu d y w ill b e a rch iv ed in th e sto ra g e fa cilitie s o f C o v a n ce -M a d iso n . A t le a st 1 y e ar a fte r su b m issio n o f th e fin al re p o rt, th e C o v a n ce A rc h iv e s sta ff w ill c o n ta c t th e S p o n so r. A t th a t tim e , th e S p o n so r m ay c h o o se to h a v e th e a fo re m e n tio n e d m a te ria ls re tu rn e d , a rc h iv e d fo r an a d d itio n a l p e rio d o f tim e , o r d isp o se d o f b y C o v a n c e ; a fee w ill b e c h a rg e d b a se d o n th e a rch iv e d isp o sitio n o p tio n se le c te d b y th e S p o n so r. R a w d a ta sto re d o n m ag n e tic m ed ia an d th e p ro to co l, stu d y c o rre sp o n d e n c e, an d th e o rig in a l fin al re p o rt w ill b e re ta in e d b y C o v an ce. A t least 1 y e a r a fte r su b m issio n o f th e fin al re p o rt, a n y fro z e n sa m p le s w ill b e re tu rn e d to th e S p o n so r, re ta in e d b y C o v a n ce , o r d isp o se d of, an d a fee w ill b e ch arg ed . D a ta g e n e ra te d b y th e S p o n so r w ill b e a rch iv ed in th e S p o n s o r's sto ra g e fa cilitie s. P C N A e v a lu a tio n d a ta , p a ra ffin b lo ck s, a n d tis su e slid es fo r P C N A w ill b e re ta in e d b y P a th o lo g y A sso c ia te s In tern a tio n a l. F ro z e n liv e r sa m p le s an d seru m sa m p le s sen t to th e S p o n so r w ill b e re ta in e d b y th e S p o n so r. RESULTS AND DISCUSSION Dose Analysis R e su lts o f th e h o m o g e n e ity , sta b ility , a n d d o se p re p a ra tio n a n a ly se s a re in T a b le s 1 th ro u g h 3. M e a n v a lu e s o f th e h o m o g e n e ity a n aly se s fo r th e m ix d o n e o n M a rc h 30, 1998, ra n g ed fro m 9 1 .3 to 1 0 6 % o f th e th e o re tic a l c o n c e n tra tio n fo r th e d iet c o n ta in in g 1 p p m N -E tF O S E . T h ese re su lts in d ic a te th a t th e m ix in g p ro c e d u re p ro d u c e d a h o m o g en e o u s d istrib u tio n o f th e te st m ate ria l in th e d o se p rep aratio n . R e su lts o f th e sta b ility a n a ly se s fo r th e m ix c o n d u c te d o n Ju n e 5, 1998, o f sa m p le s sto re d 3 2 d ay s at ro o m te m p e ra tu re c o n d itio n s in d ic a te d th a t a m ea n c o n c e n tra tio n w a s 8 1 .8 % o f th e th e o re tic a l c o n c e n tra tio n o f 1 p p m . R e su lts o f sta b ility a n a ly se s fo r th e m ix c o n d u c te d o n Ju n e 5, 1998, o f sa m p le s sto re d at ro o m te m p e ra tu re fo r 4 6 d ay s in d ic a te d th a t th e m ea n c o n c e n tra tio n w a s 9 8 .1 % o f th e th e o re tic a l c o n c e n tra tio n o f 1 ppm . T h e m ea n c o n c e n tra tio n s o f th e d o se p re p a ra tio n an aly se s fo r th e 1 -p p m lev el ra n g ed fro m 7 4 .3 to 1 2 0 % o f th e th e o re tic a l c o n c e n tra tio n (in c lu d in g re a ss a y a n d re te n tio n sa m p le an aly ses, b u t e x clu d in g d a ta th a t re q u ire d re an a ly sis). In h e re n t v a ria b ility an d 3405 Covance 6329-212 Covance 6329-228 3M T-6316.1 lac k o f su ffic ie n t se n sitiv ity at lo w lev e ls in th e a n aly tic al m e th o d fo r m ea su rin g N -E tF O S E re su lte d in a n aly se s d a ta th a t w e re in m an y c ases o u tsid e th e stan d ard ly a cc ep ta b le lim its o f + 1 5 % . Observation of Animals S u rv iv al an d C lin ic al O b serv a tio n s. C lin ical o b se rv a tio n s in clu d in g m ass o b se rv atio n s are su m m ariz ed in T ab les 5 an d 6; in d iv id u a l d a ta are in A p p e n d ix 2. S u rv iv al d a ta are illu stra te d in F ig u re s 1 a n d 2 an d su m m a riz e d in T a b le 4. S ta tistica l a n a ly se s o f su rv iv a l d a ta a re p re se n te d in th e re p o rt o f su rv iv a l a n a ly sis. In d iv id u a l a n im a l fa te d a ta a re in A p p e n d ix 2. T h irty -n in e c o n tro l m ale s an d 3 7 c o n tro l fe m a le s an d 3 0 m ale s an d 35 fe m a le s g iv en 1 p p m d ied o n te s t o r w e re sacrificed in a m o rib u n d co n d itio n . T h e m o st fre q u e n t cau se o f d e a th w a s p itu ita ry a d en o m as. T h ere w e re n o sig n ific a n t d iffe re n c e s in th e m o rta lity in b o th m ale s a n d fe m a le s. K a p la n -M e ie r e stim a te s in d ic a te th e re w a s n o tre a tm e n t-re la te d d e trim en tal e ffe c t o n th e su rv iv al o f e ith e r sex. C lin ic a l o b se rv a tio n s w e re sim ila r in n a tu re a n d in th e to ta l n u m b e r o f a ffe c te d a n im a ls in all g ro u p s. Body Weights. B o d y w e i g h t a n d b o d y w e i g h t c h a n g e d a t a a r e i l l u s t r a t e d i n F i g u r e s 3 an d 4 an d su m m ariz ed in T ab les 7 an d 8; in d iv id u a l b o d y w e ig h t d a ta are in A p p e n d ix 3. T h ere w e re n o te s t m a te ria l-re la te d effe c ts o n b o d y w e ig h ts o r b o d y w e ig h t c h an g es d u rin g th e stu d y . Food Consumption. F o o d c o n s u m p t i o n d a t a a r e i l l u s t r a t e d i n F i g u r e s 5 a n d 6 a n d su m m ariz ed in T ab le 9; in d iv id u a l d a ta are in A p p e n d ix 4. T h e re w e re n o te s t m a te ria l-re la te d e ffe c ts o n fo o d c o n su m p tio n d u rin g th e stu d y . Test Material Consumption. T e s t m a t e r i a l c o n s u m p t i o n d a t a a r e s u m m a r i z e d i n T ab le 10. In d iv id u a l te s t m ate ria l c o n su m p tio n d a ta are p re sen te d in A p p e n d ix 4. T e st m ate ria l c o n su m p tio n ra n g e d fro m 0 .0 2 9 to 0 .1 0 8 m g /k g /d a y fo r G ro u p 9. 3406 Covance 6329-212 Covance 6329-228 3M T-6316.1 Clinical Pathology H e m ato lo g y , co ag u latio n , clin ica l ch em istry , u rin aly sis, an d u rin e c h e m istry d a ta are su m m a riz e d in T a b le s 11 th ro u g h 23 in d iv id u a l d a ta a re in A p p e n d ix 5. T h e P a th o lo g y R e p o rt c o n ta in s a d isc u ssio n o f th e d ata. D ie ta ry a d m in istra tio n o f N -E tF O S E at 1 p p m fo r a p p ro x im a te ly 52 w e e k s h a d n o effects o n c lin ica l p a th o lo g y te s t re su lts. Anatomic Pathology O rg a n w e ig h t d a ta are su m m ariz ed in T ab les 2 4 th ro u g h 26 ; in cid e n ce s o f m ac ro sc o p ic an d m ic ro sc o p ic o b se rv atio n s are su m m ariz ed in T ab les 2 7 th ro u g h 36; th e in cid e n ce o f se v e rity o f se le c te d m ic ro sc o p ic o b se rv a tio n s is su m m a riz e d in T a b le s 3 7 th ro u g h 40. In d iv id u a l d a ta are in A p p e n d ix 6. T h e P a th o lo g y R e p o rt c o n ta in s a d isc u ssio n o f th e d ata. T h ere w e re n o te s t m a te ria l-re la te d ch an g e s fo r term in al b o d y w e ig h ts, a b so lu te o rg an w e ig h ts, o rg a n -to -b o d y w e ig h t p e rc e n ta g e s o r o rg a n -to -b ra in w e ig h t ra tio s fo r an y o f th e s a c rific e s . T h e in c id e n c e o f m o rta lity w a s sim ila r fo r tre a te d an d c o n tro l a n im a ls, a n d th e re w e re n o te s t m a te ria l-re la te d m a c ro sc o p ic o r m ic ro sc o p ic fin d in g s a t a n y o f th e stu d y in terv als. Proliferation Cell Nuclear Antigen Results T h e c ell p ro life ra tio n re p o rt is in A p p e n d ix 7. A fte r 14 w e e k s o n stu d y , e n h a n c e d cell p ro life ra tio n w a s n o t e v id e n t in th e liv e r o f m ale an d fe m a le ra ts e x p o se d to 1 p p m N -E tF O S E . 3407 CONCLUSION Covance 6329-212 Covance 6329-228 3M T-6316.1 M a le a n d fe m a le C rl:C D (S D )IG S B R ra ts w e re a d m in iste re d 0 o r 1 p p m o f (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l fo r at lea st 104 w eek s. T h ere w e re n o te st m a te ria l-re la te d effe c ts o n m o rta lity , c lin ic a l o b se rv a tio n s, b o d y w e ig h ts, fo o d c o n su m p tio n , clin ica l p a th o lo g y re su lts, o rg a n w e ig h ts, m ac ro sc o p ic o r m ic ro sc o p ic fin d in g s. 3408 SIGNATURES Covance 6329-212 Covance 6329-228 3M T-6316.1 3409 Covance 6329-212 Covance 6329-228 3M T-6316.1 REFERENCES Dunnett, C. W., "New Tables for Multiple Comparisons with a Control," B iom etrics, 20:482-491 (1964). Levene, H., "Robust Tests for Equality of Variances," Contributions to P robability an d Statistics, (eds.) I. Olkin et al., Ch. 25, pp. 278-292, Stanford University Press: Stanford, California (1960). Winer, B. J., "Design and Analysis of Single-Factor Experiments," S tatistical Principles in Experim ental D esign, Second Ed., Ch. 3, pp. 149-260, McGraw-Hill: New York, New York (1971a). Winer, B. J., "Analysis of Covariance," S tatistical P rin ciples in Experim ental D esign, Second Ed., Ch. 10, pp. 752-812, McGraw-Hill: New York, New York (1971b). 3410 PATHOLOGY REPORT Covance 6329-212 Covance 6329-228 3M T-6316.1 SUMMARY T h e p u rp o se o f th is stu d y w a s to a sse ss th e c a rc in o g e n ic ity o f th e te s t m ate ria l, N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l (N -E tF O S E ) w h e n a d m in iste re d in th e d iet to rats fo r at lea st 104 w eek s. T h e te s t m ate ria l w a s a d m in iste re d at a d o se lev el o f 1 ppm . D ie ta ry a d m in istra tio n o f N -E tF O S E at 1 p p m fo r a p p ro x im a te ly 52 w e e k s h a d n o effects o n c lin ica l p a th o lo g y te s t re su lts. T h ere w e re n o te s t m a te ria l-re la te d ch an g e s fo r term in al b o d y w e ig h ts, a b so lu te o rg an w e ig h ts, o rg a n -to -b o d y w e ig h t p e rc e n ta g e s o r o rg a n -to -b ra in w e ig h t ra tio s fo r an y o f th e s a c rific e s . T h e in c id e n c e o f m o rta lity w a s sim ila r fo r tre a te d an d c o n tro l a n im a ls, a n d th e re w e re n o te s t m a te ria l-re la te d m a c ro sc o p ic o r m ic ro sc o p ic fin d in g s a t a n y o f th e stu d y in terv als. METHODS T w o g ro u p s o f m ale an d fe m a le C rl:C D (S D )IG S B R ra ts (7 0 a n im a ls/se x /g ro u p ) w e re fed d iets c o n ta in in g th e te s t m ate ria l at a d o se lev el o f 0 (c o n tro l g ro u p ; re ce iv e d b asal d iet o n ly ) o r 1 ppm . T h ese g ro u p s w e re d e sig n ated as G ro u p s 8 an d 9, resp ec tiv e ly , an d th e a n im a ls w e re lo c a te d in th e sam e an im al ro o m as C o v a n ce S tu d y N o . 6 3 2 9 -2 1 2 . D u rin g W e e k s 14, 2 7 , a n d 53, b lo o d a n d u rin e w e re c o lle c te d fo r h e m a to lo g y , c lin ica l c h e m istry , u rin a ly sis, a n d u rin e c h e m istry te s ts fro m 10 a n im a ls/se x /g ro u p . B lo o d film s w e re m a d e fo r all a n im a ls s a c rific e d a t u n s c h e d u le d in te rv a ls a n d a t th e te rm in a l s a c rific e d u rin g W e e k 105. N e c ro p s ie s w e re p e rfo rm e d o n all a n im a ls th a t d ie d o r w e re sa c rific e d d u e to p o o r h e a lth , 10 a n im a ls /s e x /g ro u p sa c rific e d d u rin g W e e k 53, a n d all re m a in in g s u rv iv in g a n im a ls d u rin g W e e k 105. A t n ecro p sy , m a c ro sc o p ic o b se rv atio n s w e re re co rd e d , o rg a n w e ig h ts w e re o b tain e d (fo r sa c rifice s d u rin g W e e k 53), an d tissu e s w e re p la c e d in fix ativ e as 3411 Covance 6329-212 Covance 6329-228 3M T-6316.1 sp e c ifie d b y th e p ro to co l. A ll tis su e s fro m a n im a ls in G ro u p 8 a n d se le c te d tissu e s fro m a n im a ls in G ro u p 9 w e re e x a m in e d m ic ro sc o p ic a lly . F iv e a n im a ls/se x /g ro u p w e re sacrificed d u rin g W e e k 4 fo r h e p atic p a lm ito y l-C o A o x id ase activ ity an d d u rin g W e e k 14 fo r h e p a to c e llu la r p ro life ra tio n ra te m ea su re m e n ts a n d h e p a tic p a lm ito y l-C o A o x id a se activ ity . S ta tistica lly sig n ific a n t d iffere n ce s cite d in th e R e su lts an d D isc u ssio n se c tio n are b a se d o n c o m p a riso n s b e tw e e n th e c o n tro l an d tre a te d g ro u p s. RESULTS AND DISCUSSION Mortality T h e in c id e n c e o f m o rta lity d u rin g th e stu d y w a s sim ila r fo r tre a te d a n d c o n tro l a n im a ls. T h irty -n in e c o n tro l m a le s a n d 3 7 c o n tro l fe m a le s, a n d 3 0 m a le s a n d 35 fe m a le s g iv e n 1 p p m d ied o n te s t o r w e re sa c rific e d in m o rib u n d co n d itio n . Clinical Pathology Weeks 4 and 14 (hepatic palmitoyl-CoA oxidase activity). A d m i n i s t r a t i o n o f t h e t e s t m a te ria l h a d n o e ffe c t o n h e p a tic p a lm ito y l-C o A o x id a se activ ity . Weeks 14, 27, and 53. T h e r e w e r e s e v e r a l s m a l l , b u t s t a t i s t i c a l l y s i g n i f i c a n t , d i f f e r e n c e s fo r c lin ic a l p a th o lo g y re su lts b e tw e e n th e c o n tro l a n d tre a te d a n im a ls. N o n e o f th e se sm a ll d iffe re n c e s w e re c o n s is te n t o v e r tim e o r b e tw e e n se x e s, a n d all w e re c o n s id e re d to b e in c id e n ta l a n d u n re la te d to a d m in istra tio n o f th e te s t m ate ria l. Anatomic Pathology/All Animals Macroscopic Findings. T h e m o s t f r e q u e n t l y o b s e r v e d f i n d i n g s a t n e c r o p s y w e r e , t h i n a n im a ls, o c u la r d isc h a rg e , v e n tra l c o m p re ssio n o f th e b ra in , p itu ita ry m a sse s, a n d d a rk fo ci in th e sto m ach . O th e r fre q u e n t in cid e n ta l m ac ro sc o p ic fin d in g s fo r m ale s w e re p e rin e a l/p e ria n a l sta in in g , ro u g h su rfa ce o f th e k id n ey s, a n d d a rk fo c i in th e sto m ach . F re q u e n tly o b se rv e d fin d in g s fo r fe m a le s w e re m am m ary m asse s, o v a ria n cy st, o c u la r d isch arg e, an d n asal d isch arg e. 3412 Covance 6329-212 Covance 6329-228 3M T-6316.1 Microscopic Findings/Neoplastic Findings. T h e m o s t f r e q u e n t l y o b s e r v e d n e o p l a s m w a s p itu ita ry a d en o m a. Isle t cell a d e n o m a s o f th e p a n c re a s w e re fre q u e n tly p re se n t fo r m ales, an d c -cell a d e n o m a s o f th e th y ro id an d m a m m a ry a d en o m as, fib ro a d e n o m a s an d c a rc in o m a s w e re fre q u e n tly p re se n t fo r fe m a le s. O th e r tu m o rs w e re o n ly p re se n t in a fe w a n im a ls. Microscopic Findings/Nonneoplastic Findings. T h e m o s t f r e q u e n t l y o b s e r v e d n o n n e o p la stic fin d in g s w e re p ro te in a c e o u s c asts in th e k id n ey s, c h ro n ic p ro g ressiv e n ep h ro p ath y , h e p a to c e llu la r v a cu o latio n , v e n tra l c o m p re ssio n an d v e n tric u la r d ila tatio n o f th e b ra in , d e g e n e ra tio n /n e c ro sis o f th e h e art, a n d p itu ita ry h e m o rrh a g e . F re q u e n t m ic ro sc o p ic n o n n e o p la stic fin d in g s fo r m a le s w e re c h ro n ic in fla m m a tio n o f th e liv er, a c in a r a tro p h y o f th e p a n c re a s, fib ro sis o f th e h e art, v a c u o la tio n o f th e a d re n a l co rte x , ch ro n ic a ctiv e in fla m m a tio n o f th e p ro sta te , an d a cu te in fla m m a tio n o f th e fo o tp ad . O th e r freq u e n tly o b se rv e d n o n n e o p la stic m ic ro sc o p ic fin d in g s fo r fe m a le s w e re p elv ic m in e ra liz a tio n o f th e k id n ey s, u ltim o b ra n c h ia l cy sts o f th e th y ro id , g a la cto c ele, cy sts o f th e th y m u s, an d a n g ie c ta sis, c y stic d e g e n e ra tio n , a n d h e m o rrh a g e a n d th ro m b i o f th e a d re n a l c o rte x . Anatomic Pathology/Unscheduled Deaths T h e re w a s n o te s t m a te ria l-re la te d e ffe c t fo r m o rta lity . T h e o n ly c a u se s o f d e a th o b se rv ed in th re e o r m o re an im als w e re h e p a to c e llu la r a d e n o m a , h istio c y tic sa rco m a , an d p itu ita ry a d e n o m a fo r m ale s, an d m a m m a ry c a rc in o m a , m am m ary fib ro a d e n o m a , and p itu ita ry a d e n o m a fo r fe m a le s. B y fa r th e m o st fre q u e n t c au se o f d e a th w a s p itu ita ry a d e n o m a s. T h e c a u s e o f d e a th w a s u n d e te rm in e d fo r 11 m a le s a n d 2 fe m a le s. Macroscopic Findings. T h e r e w e r e n o t e s t m a t e r i a l - r e l a t e d m a c r o s c o p i c f i n d i n g s . Microscopic Findings. T h e r e w e r e n o t e s t m a t e r i a l - r e l a t e d m i c r o s c o p i c f i n d i n g s f o r t h e u n sc h e d u le d d e a th a n im a ls. Anatomic Pathology/Week 4 Sacrifice Organ Weights. T h e r e w e r e n o t e s t m a t e r i a l - r e l a t e d c h a n g e s f o r t e r m i n a l b o d y w e i g h t s o r a b so lu te o r re la tiv e liv e r w eig h ts. 3413 Covance 6329-212 Covance 6329-228 3M T-6316.1 Macroscopic Findings. T h e r e w e r e n o m a c r o s c o p i c f i n d i n g s p r e s e n t a t n e c r o p s y . Anatomic Pathology/Week 14 Sacrifice Organ Weights. T h e r e w e r e n o t e s t m a t e r i a l - r e l a t e d c h a n g e s f o r t e r m i n a l b o d y w e i g h t s o r a b so lu te o r re la tiv e liv e r w e ig h ts. Macroscopic Findings. T h e r e w e r e n o m a c r o s c o p i c f i n d i n g s p r e s e n t a t n e c r o p s y . Anatomic Pathology/Week 53 Sacrifice Organ Weights. T h e r e w e r e n o t e s t m a t e r i a l - r e l a t e d c h a n g e s f o r t e r m i n a l b o d y w e i g h t s o r a b so lu te o r re la tiv e liv e r w e ig h ts. Macroscopic Findings. T h e r e w e r e n o t e s t m a t e r i a l - r e l a t e d m a c r o s c o p i c f i n d i n g s . Microscopic Findings. T h e r e w e r e n o t e s t m a t e r i a l - r e l a t e d m i c r o s c o p i c f i n d i n g s . T h e m ic ro sc o p ic fin d in g s o b se rv ed at th e 5 3 -w e e k sac rifice w e re ty p ic a l o f th o se o b se rv e d as sp o n ta n e o u s fin d in g s in rats. Anatomic Pathology/Terminal Sacrifice Macroscopic Findings. T h e r e w e r e n o t e s t m a t e r i a l - r e l a t e d m a c r o s c o p i c f i n d i n g s . Microscopic Findings. T h e r e w e r e n o t e s t m a t e r i a l - r e l a t e d m i c r o s c o p i c f i n d i n g s f o r t h e te rm in a l sa c rific e an im a ls. 3414 Covance 6329-212 Covance 6329-228 3M T-6316.1 STATISTICAL REPORT OF SURVIVAL, NONNEOPLASTIC, AND NEOPLASTIC LESIONS Methods S u rv iv a l w a s a n a ly z e d u sin g life ta b le te c h n iq u e s c o n sistin g o f K a p la n -M e ie r p ro d u c t lim it e stim ates, C o x -T a ro n e b in ary re g re ssio n , an d G e h a n -B re slo w n o n p a ra m e tric m e th o d s (T h o m as, B re slo w , an d G art, 1977). C o x -T aro n e m eth o d s are w e ig h te d h e av ier to w a rd s late in c id e n c e s a n d G e h a n -B re slo w m e th o d s a re w e ig h te d to w a rd s early in c id e n c e s d u e to trea tm e n t. A s a resu lt, th e y are b o th v a lu a b le to o ls fo r th e in cid e n ce d a ta w ith o n se t tim e s. W e e k 105 w a s tre a te d as th e e n d o f th e stu d y (E O S ) in th e N a tio n a l C a n c e r In stitu te p a ck a g e fo r b o th sexes. T h e W ee k s 4, 14, an d 53 in terim sa c rific e s a n im a ls a n d W e e k 105 te rm in a l sa c rifice a n im a ls w e re c e n so re d in th e an aly sis. C o n tin u ity c o rre c te d tw o -sid e d ta il p ro b a b ility w a s e v a lu a te d at th e 5 .0 % sig n ific a n ce lev el fo r c o m p a rin g th e C o n tro l vs. T rea te d g ro u p s, as re p o rte d in T e x t T ab les 1 an d 2. K a p la n -M e ie r p ro d u c t lim it su rv iv al cu rv es are p re se n te d in F ig u re s 1 an d 2 fo r th e m ales an d fem ales, resp ectiv ely . Nonneoplastic Lesions. N o n n e o p l a s t i c l e s i o n s w e r e c h o s e n f o r s t a t i s t i c a l a n a l y s e s i f t h e in c id e n c e ra te in th e tre a te d g ro u p w a s in cre ased o r d e cre ased b y at lea st tw o o c cu rre n ce s o v e r th e c o n tro l g ro u p . T h ese w e re a n aly ze d b y o n e -sid e d F ish e r-Irw in e x ac t te s t at 5 .0 % sig n ific a n c e lev e l (T h a k u r, B e rry , a n d M ie lk e , 1 9 8 5 ), a n d th e re su lts a re re p o rte d in T ex t T ab les 3 an d 4 fo r th e m ale s an d fem ales, resp ectiv ely . Neoplastic Lesions. N e o p l a s t i c l e s i o n s w e r e c h o s e n f o r s t a t i s t i c a l a n a l y s e s i f t h e in c id e n c e ra te in th e tre a te d g ro u p w a s in cre ased o r d e cre ased b y at lea st tw o o c cu rre n ce s o v e r th e c o n tro l. T h e in c id e n ta l tu m o rs (i.e ., tu m o rs th a t w e re n o t a ssig n e d to b e th e c a u se o f d e a th o f th e an im a ls b y th e stu d y p a th o lo g ist) w e re an aly ze d b y lo g istic re g re ssio n o f tu m o r p re v alen c e (D in se an d L ag ak o s, 1983). In th is a p p ro ach , tu m o r p re v alen c e w a s m o d e le d as a lo g istic fu n c tio n o f d o se a n d tim e. B o th lin e a r a n d q u a d ra tic te rm s in tim e w e re in c o rp o ra te d in itially , a n d th e q u a d ra tic te rm w a s e lim in a te d if th e re w a s la c k o f c o n v erg e n ce . F u rth e r, in th e c a se s w h e re th e re w a s la c k o f c o n v e rg e n c e fo r th e a sy m p to tic te st o f th e lo g istic re g re ssio n m eth o d , th e e x ac t p ro b a b ility o f th e te st w a s o b tain e d b y u sin g L o g X a c t-T u rb o (1 9 9 3 ). T h e fa ta l a n d p a lp a b le (su p e rfic ia l) tu m o rs w e re a n a ly z e d b y C o x -T a ro n e b in a ry re g re ss io n m e th o d , as in th e c ase o f su rv iv a l, u sin g th e d e a th tim e o r th e first p a lp a tio n tim e ( if a p p lic a b le ) as th e tu m o r o n se t tim e. 3415 Covance 6329-212 Covance 6329-228 3M T-6316.1 T h e b e n ig n an d m alig n a n t n e o p la stic in cid e n ce s w e re e v a lu a te d sep a ra tely as w e ll as c o m b in e d w h e re a p p ro p ria te . T h e c riteria fo r c o m b in a tio n w e re b a se d o n th e w o rk o f M c C o n n e ll e t al. (1 9 8 6 ). T h e in c id e n c e s o f m u ltip le - o r g a n fin d in g s s u c h a s fib ro m a , fib ro sa rc o m a, leio m y o m a , leio m y o sa rco m a, ly m p h o m a , an d e n d o m e tria l stro m al p o ly p w e re c o u n te d b y a n im a l, n o t b y tissu e ty p e. T h e y w e re e v a lu a te d sta tistic a lly if th e y m e t th e se lec tio n c rite rio n fo r th e an aly sis. T h e sta tistical re su lts fo r th e se c ases m ay b e b ia s e d b e c a u s e n o t all th e a n im a ls w e re e x a m in e d fo r e v e ry tis su e . O n e -s id e d ta il p ro b a b ilitie s fo r g ro u p c o m p a riso n w e re e v a lu a te d at th e 5 .0 % sig n ific a n c e lev el a n d are sh o w n in T ex t T ab les 5 an d 6 fo r th e m ale s an d fem ales, resp ectiv ely . In th e n o n n e o p la stic a n aly se s, an im als sa c rifice d early (W e e k 4 ) w e re ex clu d ed . In th e sta tistical e v a lu a tio n o f th e n e o p la stic lesio n s, a n im a ls sa c rifice d early (d u rin g o r b e fo re W e e k 14) w e re d isc o u n te d fro m th e n u m b e r o f a n im a ls a t risk . O rd in a l d o se le v e ls 0 an d 1 c o rre sp o n d in g to G ro u p s 8 (C o n tro l) an d 9 (T rea te d ) w e re u se d in th e ab o v e s ta tistic a l a n a ly sis. C o n tin u ity c o rre c tio n w a s d o n e fo r all th e a s y m p to tic te s ts . T h e fo llo w in g n o tatio n s are u se d th ro u g h o u t th is rep o rt. + = E ffe c t in th e in cre ased d irec tio n - = E ffe c t in th e d e c re a se d d irec tio n E = E x act p erm u tatio n test Results and Discussion Survival. A s i n d i c a t e d b y t h e K a p l a n - M e i e r a d j u s t e d s u r v i v a l c u r v e s ( F i g u r e s 1 a n d 2 ) an d b y T e x t T ab les 1 an d 2, th e re w a s n o sig n ific a n t d iffere n ce in m o rta lity in e ith e r th e m a le s o r fe m a le s. Nonneoplastic Lesions. T h e l i v e r i n b o t h s e x e s h a s b e e n i d e n t i f i e d a s t h e o n l y o r g a n th a t is a p p ro p ria te fo r p e rfo rm in g sta tistic a l a n a ly se s fo r n o n n e o p la stic lesio n s. A s sh o w n in T ex t T ab les 3 an d 4, n o sig n ific a n t d iffere n ce s w e re n o te d in an y o f th e h e p a to c e llu la r n o n n e o p la stic lesio n s in e ith e r sex. Neoplastic Lesions. A s i n d i c a t e d i n T e x t T a b l e s 5 a n d 6 , t h e r e w e r e n o s i g n i f i c a n t in c id e n c e s o f a n y n e o p la stic fin d in g s in e ith e r sex. 3416 Covance 6329-212 Covance 6329-228 3M T-6316.1 References Dinse, G.E. and Lagakos, S.W., "Regression analysis of tumor prevalence data," Journal o f R o ya l S tatistical Society Series C (A pplied Statistics), 32: 236-248 (1983). Gart, J.J., Krewski, D., Lee, P.N., Tarone, R.E., and Wahrendorf, J., "Statistical Methods in Cancer Research," Vol. III, The D esign a n d A nalysis o f Long-Term Anim al Experim ents, IARC Scientific Publications No. 79, Oxford University Press (1986). LogXact-Turbo, Cytel Software Corporation, Version 1.1, Cambridge, Massachusetts (1993). McConnell, E.E., Solleveld, H.A., Swenberg, J.A., and Boorman, G.A., "Guidelines for combining neoplasms for evaluation of rodent carcinogenicity studies," Journal o f N ation al Cancer Institute, 76:283-289(1986). Peto, R., Pike, M. C., Day, N. E., Gray, R. G., Lee, P. N., Parish, S., Peto, J., Richards, S., and Wahrendorf, J., "Guidelines for Simple Sensitive Significance Tests for Carcinogenic Effects in Long-term Animal Experiments, in Long-term and Short-term Screening Assays for Carcinogens: A Critical Appraisal," Lyon: International A gency f o r Cancer Research, pp. 311-426 (1980). Thakur, A.K., Berry, K.J., and Mielke, P.W., Jr., "A FORTRAN program fortesting trend and homogeneity in proportions," Com puter P rogram s in Biom edicine, 19: 229-233 (1985). Thomas, D.G., Breslow, N., and Gart, J. J., "Trend and homogeneity analyses of proportions and life table data," Com puters a n d B iom edical Research, 10:373-381 (1977). 3417 T ex t T ab le 1 Covance 6329-212 Covance 6329-228 3M T-6316.1 R e su lts o f S ta tistical A n a ly se s o f S u rv iv al D a ta fo r M a le R a ts G roup U n a d ju ste d M o rta lity A d ju ste d M o rtality : K a p la n -M e ie r E stim a te (F in al) S tan d ard E rro r 8 (C o n tro l) 3 9 /7 0 (.5 5 7 ) .7 7 8 .0 5 9 9 (T rea te d ) 3 0 /7 0 (.4 2 9 ) .5 9 7 .0 6 9 G ro u p s 8 v s 9 p (tw o -sid e d ) - = E ffe c t in th e d e c re a se d d irec tio n . C ox-T arone T est .0 8 1 8 - G eh an -B reslo w T est .1 4 2 6 - T ex t T ab le 2 R e su lts o f S tatistical A n a ly se s o f S u rv iv al D a ta fo r F e m ale R a ts G roup U n a d ju ste d M o rta lity A d ju ste d M o rtality : K a p la n -M e ie r E stim a te (F in al) S tan d ard E rro r 8 (C o n tro l) 3 7 /7 0 ( 529) .7 3 6 .0 6 3 9 (T rea te d ) 3 5 /7 0 (.5 0 0 ) .6 9 8 .0 6 5 G ro u p s 8 v s 9 p (tw o -sid e d ) - = E ffe c t in th e d e c re a se d d irec tio n . C ox-T arone T est .4 4 9 2 - G eh an -B reslo w T est .2 4 9 1 - 3418 T ex t T ab le 3 Covance 6329-212 Covance 6329-228 3M T-6316.1 R e su lts o f S tatistical A n a ly se s o f N o n n e o p la stic L e sio n s in th e M a le s G roup L IV E R - H E M O R R H A G E O n e -sid ed p -v a lu e 8 (C o n tro l) 5/6 5 9 (T rea te d ) 2 /6 5 .2 2 0 0 5 - L IV E R - IN F L A M M A T IO N , C H R O N IC 1 7 /65 O n e -sid ed p -v a lu e 15 /6 5 .4 1 9 4 6 - L IV E R - V A C U O L A T IO N , H E P A T O C E L L U L A R 1 4 /65 O n e -sid ed p -v a lu e 10 /6 5 .2 4 9 1 5 - L IV E R - D E G E N E R A T IO N , C Y S T IC 5/6 5 O n e -sid ed p -v a lu e 9/6 5 .1 9 8 4 5 + L IV E R - F O C U S , A L T E R E D H E P A T O C E L L U L A R , E O S IN O P H IL IC 4 /6 5 9/6 5 O n e -sid ed p -v a lu e .1 2 0 8 1 + L IV E R - C Y S T O n e -sid ed p -v a lu e 2 /6 5 0/6 5 .2 4 8 0 6 - 3419 T ex t T ab le 4 Covance 6329-212 Covance 6329-228 3M T-6316.1 R e su lts o f S ta tistical A n a ly se s o f N o n n e o p la stic L e sio n s in th e F e m a le s G roup L IV E R - C Y S T , B IL IA R Y O n e -sid ed p -v a lu e 8 (C o n tro l) 2 /6 5 9 (T rea te d ) 0/6 5 .2 4 8 0 6 - L IV E R - IN F L A M M A T IO N , C H R O N IC 8/6 5 O n e -sid ed p -v a lu e 6/6 5 .3 8 9 1 5 - L IV E R - V A C U O L A T IO N , H E P A T O C E L L U L A R 1 4 /65 O n e -sid ed p -v a lu e 8/6 5 .1 2 0 9 1 - L IV E R - H Y P E R P L A S IA B IL E D U C T 5/6 5 O n e -sid ed p -v a lu e 7/6 5 .3 8 1 6 3 + L IV E R - F O C U S , A L T E R E D H E P A T O C E L L U L A R , B A S O P H IL IC 1 /6 5 3/6 5 O n e -sid ed p -v a lu e .3 0 9 5 6 + L IV E R - F O C U S , A L T E R E D H E P A T O C E L L U L A R , E O S IN P H IL IC 3 /6 5 7/6 5 O n e -sid ed p -v a lu e .1 6 2 0 3 + L IV E R - T E L A N G IE C T A S IS O n e -sid ed p -v a lu e 5/6 5 2 /6 5 .2 2 0 0 5 - 3420 T ex t T ab le 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 R e su lts o f S ta tistical A n a ly se s o f N e o p la stic L e sio n s in th e M a le R a ts G roup P a n c re a s - Isle t C ell, A d e n o m a F a ta l In c id e n ce In c id e n ta l In c id e n ce T o tal In c id e n ce R a te 8 (C o n tro l) 0 4 4 /6 0 9 (T rea te d ) 0 11 11/6 0 O n e -sid ed p -v a lu e .0 8 2 5 + T e stis - In te rstitia l C e ll, A d e n o m a F a ta l In c id e n ce In c id e n ta l In c id e n ce T o tal In c id e n ce R a te 0 0 0 /6 0 0 3 3/5 5 O n e -sid ed p -v a lu e .2 5 3 6 + (E ) 3421 T ex t T ab le 6 Covance 6329-212 Covance 6329-228 3M T-6316.1 R e su lts o f S tatistical A n a ly se s o f N e o p la stic L e sio n s in th e F e m a le R a ts G roup P a n c re a s - Isle t C ell, A d e n o m a F a ta l In c id e n ce In c id e n ta l In c id e n ce T o tal In c id e n ce R a te 8 (C o n tro l) 0 4 4 /5 9 9 (T rea te d ) 0 1 1 /60 O n e -sid ed p -v a lu e .1 2 0 0 - M am m ary - A denom a T o tal In c id e n ce R a te O n e -sid ed p -v a lu e 5 /6 0 2 /6 0 .1 7 9 1 - M a m m a ry - F ib ro a d en o m a T o tal In c id e n ce R a te 2 1 /6 0 2 2 /6 0 N o te : In c id e n c e s a c ro ss g ro u p s d o n o t m e e t th e se le c tio n c riterio n . M am m ary - A d e n o m a/F ib ro ad en o m a T o tal In c id e n ce R a te 2 5 /6 0 O n e -sid ed p -v a lu e 2 3 /6 0 .1 9 2 9 - M a m m a ry - C arcin o m a T o tal In c id e n ce R a te 9 /6 0 9 /6 0 N o te : In c id e n c e s a c ro ss g ro u p s d o n o t m e e t th e se le c tio n c riterio n . M a m m a ry - A d en o m a/F ib ro ad en o m a/C arcin o m a T o tal In c id e n ce R a te 2 9 /6 0 O n e -sid ed p -v a lu e 2 7 /6 0 .1 8 7 7 - 3422 Covance 6329-212 Covance 6329-228 3M T-6316.1 Figure 1 - Kaplan-Meier Survival Curves For Male Rats 0 15 30 45 60 75 90 105 ++++++++ 1 #0+********111111111111111111111 + 000000000000000000000*000000 + 111111*******000 + 1110 + 1000 .9+ 11 0 P+ 11 *0 R+ 100 O+ 11*11 P+ 001 O .8+ 0*00 R+ 110 T+ 1* I+ *11 O+ 00*1111 N .7+ 000 11 + 0 11 S+ 00 11 U+ 00 111 R+ 0 111 V .6+ 01 I+ 0 11 V+ 00 1 I+ 01 N+ 000 111 G .5+ 00 1 + 01 + 00 111 + 01 + 00 1 .4+ 000 11 + 00 + 00 +0 +0 .3+ 00 + 00 +0 +0 + 00 .2+ + + 0 = Group 8 (Control) + 1 = Group 9 (Treated) + * = More Than One Data Point 1+ + + + + 0+ ++++++++ 0 15 30 45 60 75 90 105 SURVIVAL TIME (Week) 3423 Covance 6329-212 Covance 6329-228 3M T-6316.1 Figure 2 - Kaplan-Meier Survival Curves For Female Rats 0 15 30 45 60 75 90 105 1 0+******iiiii` + 0000000000000000 ii + 00000000000000iiiiiiii + 000000 i + 0i .9+ 00 ii P+ 000* R+ *iii O+ 00 i P+ 00**i O .8+ 0*ii R+ 00iii T+ 00 iii I+ 0 ii O+ 0000 i N 7+ 00ii + 00iii S+ 00 iii U+ 0i R+ 0 iii V 6+ 0i I+ 00 0 i V+ 00 iii I+ 00 ii N+ 0i G 5+ 000 ii + 00 i + 0 ii + 00 i + 0 ii 4+ 0 i + 00 i + 0 ii + 0000ii + 0i 3+ 00ii + 00 + 00 + + 0 = Group 8 (Control) 2+ i = Group 9 (Treated) + * = More Than One Data Point + + + .i+ + + + + 0+ ++++++++ 0 i5 30 45 60 75 90 i05 SURVIVAL TIME (Week) 3424 COMMENTS ON THE DATA Covance 6329-212 Covance 6329-228 3M T-6316.1 V a rio u s m o d els o f c alcu la to rs, c o m p u te rs, an d c o m p u te r p ro g ram s w e re u se d to a n aly ze d a ta in th is stu d y . B e c a u se d iffe re n t m o d e ls ro u n d o ff o r tru n c a te n u m b e rs d iffere n tly , v a lu e s in so m e ta b le s (e .g ., m ean s, sta n d a rd d e v ia tio n s, o r in d iv id u a l v a lu e s) m ay d iffe r slig h tly fro m th o se in o th e r ta b le s, fro m in d iv id u a lly c a lc u la te d d a ta , o r fro m sta tistic a l a n a ly sis d a ta . N e ith e r th e in te g rity n o r th e in te rp re ta tio n o f th e d a ta w a s a ffe c te d b y th e s e d ifferen ces. T h e u n its fo r th e d o se lev e ls in th e in life su m m ary ta b le s are ppm . T h e n u m b e r o f a n im a ls liste d in th e h e a d in g o f th e su m m a ry ta b le s fo r c lin ica l a n d m ass o b se rv atio n s re fle c ts th e n u m b er o f a n im a ls a ssig n e d to e ach g ro u p at th e start o f th e stu d y . T h e su m m ary ta b le s fo r c lin ical o b se rv a tio n s in d ic a te s th e n u m b e r o f a n im a ls fo r w h ic h a c o n d itio n w a s o b se rv ed w ith o u t re g a rd to th e re v ersib ility , n u m b e r o f in cid e n ce s/an im al, o r th e len g th o f tim e th e c o n d itio n p ersisted . O n ly o b se rv a tio n s fo r e ach an im a l o th e r th a n n o rm al are in d ic a te d in th e su m m ary c lin ical o b se rv atio n s tab les. E a c h an im al w ith o b se rv atio n s re c o rd e d as "N o rm a l" th ro u g h o u t th e stu d y h a s th e c o m m e n t "N o re m a rk ab le o b se rv a tio n s" in d ic a te d in th e in d iv id u a l clin ica l o b se rv atio n s tab les. T h e sp ecific d e ta ils fo r c o m m e n ts in th e in d iv id u a l c lin ical o b se rv atio n s ta b le s th a t are in d ic a te d w ith a " C " can b e fo u n d at th e en d o f e ach g ro u p fo r e ach sex. F o o d c o n su m p tio n v a lu e s are re p o rte d in w h o le n u m b e rs; h o w e v er, th e d a ta co lle ctio n sy ste m c arries fo o d c o n su m p tio n v a lu e s to o n e p lace to th e rig h t o f th e d ecim al fo r te st m ate ria l c o n su m p tio n calcu latio n s. T h e d iffere n ce s in v a lu e s g e n e ra te d d o n o t in flu e n ce th e in te rp re ta tio n o f th e c alcu la tio n . T h e c o m m e n t "S P IL L E D " in in d iv id u a l fo o d c o n su m p tio n d a ta tab le s in d ic a te s th a t fo o d c o n su m p tio n w a s n o t re c o rd e d d u e to sp illa g e d u rin g th e in terv al. T h e c o m m e n t "N O T T A K E N " in in d iv id u a l fo o d c o n su m p tio n d a ta ta b le s in d ic a te s th a t th e a n im a l d ie d b e fo re th e e n d o f th e fo o d c o n su m p tio n in te rv al. D iffe re n c e s in th e p o p u la tio n size (N ) in th e su m m ary ta b le s fo r c lin ica l a n d a n a to m ic p a th o lo g y a re e x p la in e d in th e in d iv id u a l d a ta ta b le s o r th e c o d e s sh e e ts o r in th e P ro to c o l D e v ia tio n s se ctio n . 3425 COMMENTS ON THE DATA (Continued) Covance 6329-212 Covance 6329-228 3M T-6316.1 R e su lts o f c lin ica l p a th o lo g y sa m p le s c o lle c te d fo r an im als sa c rifice d at u n sc h e d u le d in te rv als a p p ea r in th e in d iv id u a l clin ica l p a th o lo g y ta b le s fo r th e n e x t sc h e d u le d in terv al. T h ese re su lts are n o t in th e su m m ary ta b le s an d are n o t in clu d e d in th e sta tistical an aly ses. S ta tistica l a n aly se s w e re n o t d o n e w h e n th e re w e re tw o o r fe w er v a lu e s fo r a p a ram ete r. T h e c a lc u la tio n fo r in d iv id u a l te s t m ate ria l c o n su m p tio n is: T e st m aterial c o n su m p tio n = Diet ConcentrationxFood Consumption Food Consumption Intervalx[First Body Weight of Interval+ (Daily Body Weight GainxDay Factor)] w h ere: D a ily B o d y W e ig h t G ain = Last Body Weight of Interval- First Body Weight of Interval Day of Last Body Weight of Interval- Day of First Body Weight of Interval and: D ay F a c to r = Food Consumption Interval (Food Consumption Start Day- Day of First Body Weight of Interval) + 2 and: F o o d C o n su m p tio n In terv a l = Food Consumption End Day- Food Consumption Start Day 3426 CODES, ABBREVIATIONS, AND UNITS Covance 6329-212 Covance 6329-228 3M T-6316.1 G en eral C o d e s an d A b b re v iatio n s C o d e s fo r C lin ical P a th o lo g y A b b re v ia tio n s a n d U n its fo r C lin ic al H e m a to lo g y A b b re v ia tio n s a n d U n its fo r C lin ic al C h e m istry A b b re v ia tio n s a n d U n its fo r C lin ic al U rin a ly sis A b b re v ia tio n s an d U n its fo r U rin e C h e m istry C o d es fo r A n ato m ic P a th o lo g y N o te: T h e fo llo w in g lists o f co d es, ab b rev iatio n s, an d u n its are u se d b y C o v a n c e . S o m e , b u t n o t n e c e s s a rily all, o f th is in fo rm a tio n m a y b e n e ed e d fo r th is re p o rt. 3427 General Codes and Abbreviations Covance 6329-212 Covance 6329-228 3M T-6316.1 WK N M ean; M E A N CAM S D ; S .D .; S T A N D D E V ; S T A N D A R D D E V ; sd * -; N A P C UN SCHED D IS P A T C H W eek N u m b e r o f m e a su re m e n ts in a g ro u p A rith m e tic m ean C o v a ria te -a d ju ste d m ean S ta n d ard d e v ia tio n G ro u p m e a n is sig n ific a n tly d iffe re n t fro m th e m ea n o f th e c o n tro l g ro u p (G ro u p 1) at p < 0 .0 5 N o v a lu e ; n o t a p p lic a b le ; n o t p re se n t P resent C o m m en t fo u n d at th e end o f each g ro u p fo r each sex U n sch ed u led O b se rv a tio n s tra n sfe rre d fro m th e in life m o d u le o f th e d a ta c o lle ctio n sy ste m to th e n e c ro p sy m o d u le fo r re fe re n ce d u rin g n ecro p sy . O b se rv a tio n s are d u p lic a te s o f th e last in life o b serv atio n s. Animal Death Codes 1 2 3 T D M In terim sacrifice, W e e k 4 In terim sacrifice, W e e k 14 In terim sacrifice, W e e k 52 T erm in al sa c rifice Found dead M o rib u n d 3428 NS Q S /Q N S NR FS SC SH H SL L SI I NF U D T /D O T DB TJ TE RE EE SE PC PD PI PL PA CO HB PLA SM O NO AGG FR UTD NO COAG Codes for Clinical Pathology GENERAL CODES Covance 6329-212 Covance 6329-228 3M T-6316.1 N o sam p le Q u a n tity n o t su fficien t N o re p e a t (sa m p le v o lu m e n o t su fficien t fo r re p e a t a n aly sis) F ib rin stran d s S a m p le c lo tte d S lig h tly h e m o ly z e d H e m o ly z ed S lig h tly lip e m ic L ip e m ic S lig h tly icte ric Ic te ric A n im al n o t fasted U n sch e d u led /m o rib u n d b leed A n im al d ied o n te st D ie d d u rin g b lee d in g T e c h n ic ia n ju d g m e n t to re p ea t test T e c h n ic a l e rro r (in stru m e n t o r te c h n ic ia n e rro r th a t re su lts in u n a c c e p ta b le d a ta , e .g ., u n a c c e p ta b le in stru m e n t o u tp u t, sa m p le sp illed , e n try o f in v alid d a ta ) R e c o rd in g e rro r (re c o rd e d in c o rre c t d a ta , e .g ., w ro n g n u m b e r, sp ellin g e rro r, in c o rre c t d a te ) E n try e rro r (in c o rre c t k e y b o a rd en try ) S a m p lin g e rro r P la te le ts c lu m p ed P la te le ts d e cre ased P la te le ts in cre ased P la te le ts larg e P la te le ts a p p e a r a d eq u a te C o lo r in te rfere s w ith te st H e in z b o d ies o b serv ed P la sm o d iu m N o a g g reg a tio n F ra c tio u s U n a b le to d ete rm in e N o co ag u latio n 3429 Covance 6329-212 Covance 6329-228 3M T-6316.1 Codes for Clinical Pathology (Continued) RESULTS NOT INCLUDED IN STATISTICAL ANALYSES H e m o ly z e d clin ica l c h e m istry o r c o ag u la tio n sam p les S am p les fro m a n im a ls at u n sc h e d u le d in te rv als P ro th ro m b in tim e s (P T ) g re a te r th a n 50 seco n d s A c tiv a te d p a rtial th ro m b o p la stin tim e s (P T T ) g re a te r th a n 110 seco n d s B le e d tim e s (B L E T IM E ) g re a te r th a n 3 0 m in u te s CODES FOR BLOOD CELL MORPHOLOGY T h e fo llo w in g sc a le w a s u se d to m e a su re th e d e g re e o f a n iso c y to sis (A N IS O ), p o ik ilo c y to sis (P O IK ), p o ly c h ro m a sia (P O L Y ), h y p o c h ro m a sia (H Y P O ), o r b a so p h ilic stip p lin g (B A S T IP ) o r th e p re sen c e o f H o w e ll-Jo lly b o d ie s (H JB O D Y ), to x ic n e u tro p h ils (T O X N E U T ), o r aty p ical ly m p h o c y te s (A T Y P L Y M ). S cale 1 2 3 4 D egree N o rm a l fo r th e sp e c ie s S lig h t M o d erate M arked N o t ap p lic ab le P resence N o t present R are Few M o d erate M any URINE APPEARANCE A P ale B S traw C Y e llo w D D a rk y e llo w C o lo r E A m ber F B row n G R ed H G reen I B lack P B lu e/g re en Q B lu e R O range C larity J C lear K H azy L C lo u d y M isc e lla n e o u s M D eb ris O Feces 3430 Codes for Clinical Pathology (Continued) URINE CHEMISTRY MULTISTIX STRIP Covance 6329-212 Covance 6329-228 3M T-6316.1 U rin e G lu co se + ++ +++ ++++ +++++ N eg ativ e 100 m g /d L 2 5 0 m g /d L 5 0 0 m g /d L 1 ,0 0 0 m g /d L > 2 ,0 0 0 m g /d L U rin e K eto n e + ++ +++ ++++ +++++ N eg ativ e 5 m g /d L 15 m g /d L 4 0 m g /d L 8 0 m g /d L 160 m g /d L U rin e B lo o d + ++ +++ N eg ativ e S m all M o d erate L arge U rin e U ro b ilin o g e n + ++ +++ ++++ 0 .2 m g /d L 1 m g /d L 2 m g /d L 4 m g /d L 8 m g /d L (1 m g = a p p ro x im a te ly 1 E h rlic h u n it) U rin e B iliru b in + ++ +++ N eg ativ e S m all M o d erate L arge URINE SEDIMENT Cells, Crystals, Casts, and Comments A A m o rp h o u s u ra te s B A m o rp h o u s p h o sp h a tes C U ric acid D T rip le p h o sp h a te s E C a lc iu m o x a la te F C a lc iu m c arb o n a te G G ra n u la r casts H H y a lin e c asts I C e llu lar casts J W a x y c asts K U n k n o w n cry stal P M u c o u s th rea d s Q S perm R F e c al c o n ta m in a tio n S P in w o rm o v a fo u n d T P in w o rm larv ae fo u n d U P a ra site o v a fo u n d 0 N o t present 1 1 - 5 p e r field 2 6 - 1 0 p e r field 3 1 1 - 2 0 p e r field 4 > 2 0 p e r field Bacteria 0 N o t present 1 Few 2 M o d erate 3 M any 3431 Covance 6329-212 Covance 6329-228 3M T-6316.1 Abbreviations and Units for Clinical Hematology Test R e d b lo o d cell c o u n t H e m o g lo b in H em ato crit M e a n c o rp u scu la r v o lu m e M e a n c o rp u scu la r h em o g lo b in M e a n c o rp u sc u la r h e m o g lo b in c o n c e n tra tio n P la te le t co u n t W h ite b lo o d cell c o u n t D iffe re n tia l b lo o d cell c o u n t S e g m en te d n e u tro p h il c o u n t L y m p h o cy te co u n t M o n o cy te co u n t E o sin o p h il c o u n t B a so p h il c o u n t A n iso c y to sis P o ly ch ro m asia P o ik ilo c y to sis H y p o ch ro m asia T o x ic n e u tro p h ils Abbreviation (Units) R B C ( E 6 / U L o r X 1 0 6/ / / L ) H G B (G /D L ) H C T (% ) M C V (F L ) M C H (P G ) M C H C (% ) P L T ( E 3 / U L o r X 1 0 3/ / / L ) W B C ( E 3 / U L o r X 1 0 3/ / / L ) N - S E G ( E 3 / U L o r X 1 0 3/ / / L ) a n d % L Y M P H ( E 3 / U L o r X 1 0 3/ / / L ) a n d % M O N O ( E 3 / U L o r X 1 0 3/ / / L ) a n d % E O S I N ( E 3 / U L o r X 1 0 3/ / / L ) a n d % B A S O ( E 3 / U L o r X 1 0 3/ / / L ) a n d % A N IS O (-,1 ,2 ,3 ) P O L Y (-,1 ,2 ,3 ) P O IK (-,1 ,2 ,3 ) H Y P O (-,1 ,2 ,3 ) T O X N E U T (-,1 ,2 ,3 ,4 ) 3432 Covance 6329-212 Covance 6329-228 3M T-6316.1 Abbreviations and Units for Clinical Chemistry Test G lu c o se U re a n itro g e n C re atin in e T o tal p ro tein A lb u m in G lo b u lin T o tal b iliru b in C h o leste ro l T rig ly c erid e A sp artate a m in o tran sferase A la n in e a m in o tran sfe ra se G a m m a g lu ta m y ltra n sfe ra se P alm ito y l C o A o x id ase C alciu m In o rg an ic p h o sp h o ru s S o d iu m P o tassiu m C h lo rid e Abbreviation (Units) G L U (M G /D L ) U N (M G /D L ) C R E A T (M G /D L ) T P R O (G /D L ) A L B (G /D L ) G L O B (G /D L ) T B IL I (M G /D L ) C H O L (M G /D L ) T R IG (M G /D L ) A S T /S G O T (IU /L ) A L T /S G P T (IU /L ) G G T (IU /L ) P C O A O (IU /G ) C A (M G /D L ) I P H O S (M G /D L ) N A (M M O L /L ) K (M M O L /L ) C L (M M O L /L ) 3433 Covance 6329-212 Covance 6329-228 3M T-6316.1 Abbreviations and Units for Clinical Urinalysis Test U rin e v o lu m e S p ecific g ra v ity U rin e c h e m istry M u ltistix strip U rin e p H U rin e p ro tein U rin e g lu co se U rin e k e to n e s U rin e b iliru b in U rin e b lo o d U rin e u ro b ilin o g e n M ic ro sc o p ic e x a m in a tio n o f u rin e se d im e n t R e d b lo o d cells p e r h ig h -p o w e r field W h ite b lo o d cells p e r h ig h -p o w e r field E p ith e lial c ells p e r h ig h -p o w e r field B a c te ria p e r h ig h -p o w e r field C a sts p e r lo w -p o w e r field C ry sta ls p e r lo w -p o w e r field U rin e ap p earan ce C o m m en ts Abbreviation (Units) U V O L (M L ) SPG R U PH U P R O (M G /D L ) U GLU U K ET U B IL I U BLOOD U R O B IL I R B C (P E R H P F ) W B C (P E R H P F ) E P IT H (P E R H P F ) B A C T (P E R H P F ) C A S T S (P E R L P F ) C R Y S T A L S (P E R L P F 1 or PE R LPF2) U R IN E A P P I o r U R IN E A P P 2 COM M ENTS Abbreviations and Units for Urine Chemistry Test U rin e so d iu m U rin e p o tassiu m S o d iu m e x cre tio n P o ta ssiu m e x cre tio n Abbreviation (Units) U N A (M M O L /L ) U K (M M O L /L ) N A E X C (M M O L ) K E X C (M M O L ) 3434 Code EX NOT TAKEN M IS S IN G U N S U IT A B L E A U T O L Y T IC EXCLUDE Codes for Anatomic Pathology Definition MACROSCOPIC CODES Covance 6329-212 Covance 6329-228 3M T-6316.1 In d ic a te s th a t o rg a n w e ig h t is e x c lu d e d fro m c a lc u la tio n s O rg a n w e ig h t n o t tak e n ; e x p la n a tio n g iv e n in n e c ro p sy n o tes O rg a n m issin g o r lo st O rg a n te c h n ic a lly u n su ita b le fo r w e ig h in g O rg a n a u to ly z ed an d c o u ld n o t b e w e ig h ed W e ig h t w a s ta k e n , b u t w a s e x c lu d e d fro m all c a lc u la tio n s MICROSCOPIC CODES Codes Prefacing Neoplastic Findings B - P rim ary , b e n ig n n e o p la sm M - P rim ary , m alig n a n t n e o p la sm N - M e ta sta tic n e o p la sm I- L o c a lly in v a siv e n e o p la sm X - O th e r n e o p la sm Distribution of Findings Focal D iffu se M u ltifo c a l Grades for Severity or Amount 1 M in im a l - th e least am o u n t o f ch an g e th a t can b e o b se rv ed w ith th e lig h t m ic ro sc o p e 2 S lig h t - less th a n a v era g e a m o u n t o f c h an g e, b u t re ad ily d isc ern ib le as abnorm al 3 M o d e ra te - th e a v e ra g e a m o u n t o f c h a n g e th a t is e x p e c te d fo r a lesio n 4 M o d e ra te ly se v e re (m ark e d ) - a m ark e d a m o u n t o f c h an g e w ith p o ssib le lo ss o f fu n c tio n o f th e a ffe c te d cells o r o rg a n s 5 S ev ere - a g re a t am o u n t o f c h an g e w ith p ro b a b le lo ss o f fu n c tio n o f th e a ffe c te d cell o r o rg a n s a n d fre q u e n tly in v o lv e s la rg e a re a s o f th e o rg an 3435 Codes for Anatomic Pathology (Continued) Other Microscopic Codes T L T o ta l P F in d in g p resen t - F in d in g n o t p resen t MN M ean Covance 6329-212 Covance 6329-228 3M T-6316.1 TISSUE ABBREVIATIONS Abbreviation LN GL STOM ACH, GL STOM ACH, N O N G L S A L IV G L , M A N D IB L N , A N T M E S /P A N C A U D IT O R Y SE B G L L A C R IM A L G L A N D , E X H E M A T O N E O P L A S IA L A C R IM A L G L , IN T C A V IT Y , A B D O M S A L IV G L ,P A R O T ID LN, TRACHEOBRON Definition Lym ph node G lan d G la n d u la r sto m ac h N o n g la n d u la r sto m ach M a n d ib u la r saliv ary g la n d A n te rio r m e se n te ric /p a n c re a tic ly m p h n o d e A u d ito ry se b a ce o u s g lan d E x o rb ita l lac rim a l g lan d H e m a to p o ie tic n e o p la sia In tern a l lac rim a l g lan d A b d o m in a l cav ity P a ro tid saliv ary g lan d T rach eo b ro n ch ial ly m p h n o d e 3436 Covance 6329-212 Figure 1 Adjusted Survival Data (%) - Males 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS Covance 6329-228 3M T-6316.1 3437 Covance 6329-212 Figure 2 Adjusted Survival Data (%) - Females Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS Week 3438 Covance 6329-212 Figure 3 Mean Body Weight Data (g) - Males Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) 105 3439 Covance 6329-212 Figure 4 Mean Body Weight Data (g) - Females Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) 105 3440 225 200 175 150 125 100 75 1 Covance 6329-212 Figure 5 Mean Food Consumption Data (g) - Males Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS 0 ppm 1 ppm 11 13 15 20 28 36 44 52 60 68 76 84 92 100 106 Week 3441 Covance 6329-212 Figure 6 Mean Food Consumption Data (g) - Females Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO IN RATS 3442 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 1 R e su lts o f H o m o g e n e ity A n a ly se s (p p m ) M ix e d 3 /3 0 /9 8 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S S a m p le L o c a tio n T -6 3 1 6 (ppm ) R e p lic a te 1 Top 1 2 3 M ean 0 .8 2 4 1 .0 1 0 .9 0 6 0 .9 1 3 (9 1 .3 )a M id d le 1 2 3 M ean 1 .0 2 0 .8 9 4 0 .9 0 0 0 .9 3 8 (9 3 .8 ) B o tto m 1 2 3 M ean 0 .7 6 6 1 .2 8 1 .13 1 .0 6 (1 0 6 ) a E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th eo retical. 3443 T ab le 2 Covance 6329-212 Covance 6329-228 3M T-6316.1 R e su lts o f S tab ility A n a ly se s (p p m ) M ix e d 3 /3 0 /9 8 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S In terv al R e p lic a te T -6 3 1 6 (ppm ) 1 In itia la 1 2 3 M ean 1 .0 2 0 .8 9 4 0 .9 0 0 0 .9 3 8 (9 3 .8 )b 21 days at room tem p eratu re 1 2 M ean 0 .8 9 2 0 .8 4 2 0 .8 6 7 (8 6 .7 ) 32 days at room tem p eratu re 1 2 M ean 1 .3 9 1 .2 0 1 .3 0 (1 3 0 ) a R e su lts w e re d e riv e d fro m th e sa m p le c o lle cted fro m th e m id d le o f th e d o se p re p ara tio n s fo r h o m o g e n e ity an aly sis. b E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th eo retical. 3444 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 2 (C o n tin u e d ) R e su lts o f S tab ility A n a ly se s (p p m ) M ix e d 6 /5 /9 8 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S In terv al T -6 3 1 6 (ppm ) R e p lic a te 1 In itia l 1 2 M ean 0 .9 6 6 1 .13 1 .0 5 (1 0 5 )b 32 days at room tem p eratu re 1 2 M ean 0 .8 2 8 0 .8 0 8 0 .8 1 8 (8 1 .8 ) 46 days at room tem p eratu re 1 2 M ean 1 .0 6 0 .9 0 2 0 .9 8 1 (9 8 .1 ) b E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th eo retical. 3445 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 3 R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek T -6 3 1 6 (ppm ) R e p lic a te 0 1 1a 1b 1 .0 2 2b 0 .8 9 4 3- 0 .9 0 0 M ean - 0 .9 3 8 (9 3 .8 )c 2 -5 2-5d 1 2 M ean 1 2 M ean b b - - 0 .7 8 2 0 .6 6 8 0 .7 2 5 (7 2 .5 ) 1 .1 2 1 .0 4 1 .0 8 (1 0 8 ) 6 -9 6 -9 d 1 2 M ean 1 2 M ean 0 .8 4 8 b - 1 .4 8 1 .53 1 .51 (1 5 1 ) b 0 .8 9 0 b 0 .8 5 8 - 0 .8 7 4 (8 7 .4 ) a R e su lts fo r th e 1 p p m lev el w e re d eriv ed fro m th e sam p le c o lle c te d fro m th e m id d le o f th e d o se p re p ara tio n s fo r h o m o g en e ity an aly sis. b B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ). c E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e th eo re tic al. d R eassay. 3446 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 3 (C o n tin u e d ) R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek T -6 3 1 6 (ppm ) R e p lic a te 0 1 1 0 -1 3 1 2 M ean b b - 0 .9 6 6 1 .13 1 .0 5 (1 0 5 )c 1 4 -1 7 1 2 M ean b b - 0 .8 9 4 0 .8 7 0 0 .8 8 2 (8 8 .2 ) 1 8 -2 1 1 8 -2 1 d 1 8 -2 1 e 1 2 M ean 1 2 M ean 1 2 M ean b b - - - 1 .6 9 1 .0 7 1 .3 8 (1 3 8 ) 0 .8 8 0 1 .2 6 1 .0 7 (1 0 7 ) 0 .7 8 9 0 .7 0 4 0 .7 4 7 (7 4 .7 ) 2 2 -2 5 1 2 M ean b b - 0 .7 9 8 0 .8 7 6 0 .8 3 7 (8 3 .7 ) b Below the limit o f quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. e Retention. 3447 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 3 (C o n tin u e d ) R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek T -6 3 1 6 (ppm ) R e p lic a te 0 1 26-29 2 6 -29d 26-29e 1 2 M ean 1 2 M ean 1 2 M ean b b - - - 8 .8 1 1 .8 8 5 .3 5 (5 3 5 )c 0 .7 3 0 0 .7 8 6 0 .7 5 8 (7 5 .8 ) 0 .9 0 2 0 .9 3 6 0 .9 1 9 (9 1 .9 ) 3 0 -3 3 30-33d 1 2 M ean 1 2 M ean b b - - 1.0 5 1 .3 2 1 .1 9 (1 1 9 ) 0 .9 5 1 0 .9 5 5 0 .9 5 3 (9 5 .3 ) 3 4 -3 7 1 2 M ean b b - 1 .0 2 1 .0 0 101 (101) b Below the limit o f quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. e Retention. 3448 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 3 (C o n tin u e d ) R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek T -6 3 1 6 (ppm ) R e p lic a te 0 1 3 8 -4 1 1 2 M ean b b - 0 .8 3 6 1 .1 6 0 .9 9 8 (9 9 .8 )c 4 2 -4 5 1 2 M ean b b - 0 .9 1 2 0 .8 8 6 0 .8 9 9 (8 9 .9 ) 46-49 4 6 -49d 4 6 -49e 1 2 M ean 1 2 M ean 1 2 M ean b b - - - 0 .7 8 1 0 .7 0 5 0 .7 4 3 (7 4 .3 ) 0 .6 4 8 0 .6 6 9 0 .6 5 9 (6 5 .9 ) 0 .5 6 8 0 .5 7 3 0 .5 7 1 (5 7 .1 ) 5 0 -5 3 1 2 M ean b b - 0 .8 9 5 2 .1 8 1 .5 4 (1 5 4 ) b Below the limit o f quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. e Retention. 3449 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 3 (C o n tin u e d ) R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek T -6 3 1 6 (ppm ) R e p lic a te 0 1 50-53d 1 2 M ean - 0 .9 1 6 0 .9 0 4 0 .9 1 0 (9 1 .0 )c 5 4 -5 7 5 4 /5 7 d 5 4 /5 7 e 1 2 M ean 1 2 M ean 1 2 M ean b b - - - 0 .9 7 7 0 .7 8 4 0 .8 8 1 (8 8 .1 ) 0 .7 7 5 0 .7 4 2 0 .7 5 9 (7 5 .9 ) 0 .4 4 4 0 .5 0 1 0 .4 7 3 (4 7 .3 ) 5 8 -6 1 1 2 M ean b b - 1 .0 9 0 .9 4 5 1 .0 2 (1 0 2 ) 5 8 -6 1 d 1 2 M ean - 0 .7 8 2 0 .9 8 7 0 .8 8 5 (8 8 .5 ) 5 8 -6 1 e 12- 1.0 5 0 .9 3 8 M ean - 0 .9 9 4 (9 9 .4 ) b B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ). c E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e th eo re tic al. d R eassay. e R eten tio n . 3450 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 3 (C o n tin u e d ) R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 T -6 3 1 6 (ppm ) 1 6 2 -6 5 62-65d 6 2 -6 5 e 1 2 M ean 1 2 M ean 1 2 M ean b b - - - 0 .8 1 3 1 .1 8 0 .9 9 7 (9 9 .7 )c 0 .7 2 7 0 .6 7 9 0 .7 0 3 (7 0 .3 ) 0 .7 1 6 0 .8 7 2 0 .7 9 4 (7 9 .4 ) 6 6 -6 9 1 2 M ean b b - 0 .7 0 0 0 .7 8 6 0 .7 4 3 (7 4 .3 ) 66-69d 1 2 M ean - 0 .9 6 9 0 .7 6 3 0 .8 6 6 (8 6 .6 ) 6 6 -6 9 e 1 2 M ean - 0 .5 4 9 0 .4 9 0 0 .5 2 0 (5 2 .0 ) b Below the limit o f quantitation (<0.5 ppm). c Each value in parentheses is the percent of the theoretical. d Reassay. e Retention. 3451 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 3 (C o n tin u e d ) R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 T -6 3 1 6 (ppm ) 1 7 0 -7 3 70-73d 1 2 M ean 1 2 M ean b b - - 0 .8 8 9 0 .7 8 0 0 .8 3 5 (8 3 .5 )c 0 .4 9 8 0 .4 7 8 0 .4 8 8 (4 8 .8 ) 7 4 -7 7 74-77d 1 2 M ean 1 2 M ean b b - - 1 .2 2 1 .1 7 1 .2 0 (1 2 0 ) 0 .5 9 2 0 .8 0 6 0 .6 9 9 (6 9 .9 ) b B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ). c E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e th eo re tic al. d R eassay. 3452 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 3 (C o n tin u e d ) R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 T -6 3 1 6 (ppm ) 1 7 8 -8 1 1 2 M ean b b - 0 .8 5 6 1 .0 1 0 .9 3 3 (9 3 .3 )c 8 2 -8 5 1 2 M ean b b - 0 .5 8 2 0 .6 3 5 0 .6 0 9 (6 0 .9 ) 8 6 -8 9 1 2 M ean b b - 0 .9 7 0 1 .13 1 .0 5 (1 0 5 ) 9 0 -9 3 9 0 -9 3 d 1 2 M ean 1 2 M ean b b - - 0 .7 6 5 0 .8 8 6 0 .8 2 6 (8 2 .6 ) 3 .2 2 3 .1 9 3 .2 1 (3 2 1 ) 9 4 -9 7 9 4 -9 7 d 1 2 M ean 1 2 M ean b b - - 0 .7 5 6 0 .9 1 9 0 .8 3 8 (8 3 .8 ) 0 .6 7 7 0 .6 8 3 0 .6 8 0 (6 8 .0 ) b B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ). c E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e th eo re tic al. d R eassay. 3453 Covance 6329-212 Covance 6329-228 3M T-6316.1 T ab le 3 (C o n tin u e d ) R e su lts o f D o se P re p a ra tio n A n a ly se s (p p m ) 104-W E E K D IE T A R Y C A R C IN O G E N IC IT Y S T U D Y W IT H N A R R O W R A N G E (9 8 .1 % ) N -E T H Y L P E R F L U O R O O C T A N E S U L F O N A M ID O E T H A N O L IN R A T S W eek R e p lic a te 0 T -6 3 1 6 (ppm ) 1 94-97e 1 2 M ean - 0 .6 5 8 0 .6 2 7 0 .6 4 3 (6 4 .3 ) 9 8 -1 0 1 1 2 M ean b b - 0 .8 0 6 0 .9 2 3 0 .8 6 5 (8 6 .5 ) 1 0 2 -1 0 4 1 2 M ean b b - 1 .1 6 1 .0 7 1 .1 2 (1 1 2 ) b B e lo w th e lim it o f q u a n tita tio n (< 0 .5 p p m ). c E a c h v a lu e in p a re n th e se s is th e p e rc e n t o f th e th eo re tic al. e R eten tio n . 3454 Covance 6329-212 GROUP AND DOSE LEVEL (PPM) 8 0 9 1 8 0 9 1 WEEK: START 70/70 100% 70/70 100% 70/70 100% 70/70 100% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 1 70/70 100% 70/70 100% 70/70 100% 70/70 100% 2 70/70 100% 70/70 100% 70/70 100% 70/70 100% 3 70/70 100% 70/70 100% 70/70 100% 70/70 100% 45 MALE 65/65 100% 65/65 100% FEMALE 65/65 100% 65/65 100% 65/65 100% 65/65 100% 65/65 100% 65/65 100% 6 65/65 100% 65/65 100% 65/65 100% 65/65 100% 7 65/65 100% 65/65 100% 65/65 100% 65/65 100% 8 65/65 100% 65/65 100% 64/65 98% 65/65 100% 9 65/65 100% 65/65 100% 64/65 98% 65/65 100% Covance 6329-228 3M T-6316.1 PAGE: 1 10 11 65/65 100% 65/65 100% 64/6 98% 65/6 100% 64/65 98% 65/65 100% 64/6 98% 65/6 100% 3455 Covance 6329-212 GROUP AND DOSE LEVEL (PPM) 8 0 9 1 8 0 9 1 WEEK: 12 64/65 98% 65/65 100% 64/65 98% 65/65 100% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 13 64/65 98% 65/65 100% 64/65 98% 65/65 100% 14 59/60 98% 60/60 100% 59/60 98% 60/60 100% 15 59/60 98% 60/60 100% 59/60 98% 60/60 100% 16 17 MALE 59/60 98% 60/60 100% FEMALE 59/60 98% 60/60 100% 59/60 98% 60/60 100% 59/60 98% 60/60 100% 18 59/60 98% 60/60 100% 59/60 98% 60/60 100% 19 59/60 98% 60/60 100% 59/60 98% 60/60 100% 20 59/60 98% 60/60 100% 59/60 98% 60/60 100% 21 59/60 98% 60/60 100% 59/60 98% 60/60 100% Covance 6329-228 3M T-6316.1 PAGE: 2 22 23 59/60 98% 60/60 100% 59/60 98% 60/60 100% 59/60 98% 60/60 100% 59/60 98% 60/60 100% 3456 Covance 6329-212 GROUP AND DOSE LEVEL (PPM) 8 0 9 1 8 0 9 1 WEEK: 24 59/60 98% 60/60 100% 59/60 98% 60/60 100% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 25 59/60 98% 60/60 100% 59/60 98% 60/60 100% 26 59/60 98% 60/60 100% 59/60 98% 60/60 100% 27 59/60 98% 60/60 100% 59/60 98% 60/60 100% 28 29 MALE 59/60 98% 60/60 100% FEMALE 59/60 98% 60/60 100% 59/60 98% 60/60 100% 59/60 98% 60/60 100% 30 59/60 98% 60/60 100% 58/60 97% 60/60 100% 31 59/60 98% 60/60 100% 58/60 97% 60/60 100% 32 59/60 98% 60/60 100% 58/60 97% 60/60 100% 33 59/60 98% 60/60 100% 58/60 97% 60/60 100% Covance 6329-228 3M T-6316.1 PAGE: 3 34 35 59/60 98% 60/60 100% 59/6 98% 60/6 100% 58/60 97% 60/60 100% 58/6 97% 60/6 100% 3457 Covance 6329-212 GROUP AND DOSE LEVEL (PPM) 8 0 9 1 8 0 9 1 WEEK: 36 59/60 98% 60/60 100% 58/60 97% 60/60 100% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 37 59/60 98% 60/60 100% 58/60 97% 60/60 100% 38 59/60 98% 60/60 100% 58/60 97% 60/60 100% 39 59/60 98% 60/60 100% 58/60 97% 60/60 100% 40 41 MALE 59/60 98% 60/60 100% FEMALE 58/60 97% 60/60 100% 59/60 98% 60/60 100% 58/60 97% 60/60 100% 42 59/60 98% 58/60 97% 58/60 97% 60/60 100% 43 59/60 98% 58/60 97% 58/60 97% 60/60 100% 44 59/60 98% 58/60 97% 58/60 97% 60/60 100% 45 59/60 98% 58/60 97% 58/60 97% 60/60 100% Covance 6329-228 3M T-6316.1 PAGE: 4 46 47 59/60 98% 58/60 97% 59/6 98% 58/6 97% 58/60 97% 60/60 100% 58/6 97% 60/6 100% 3458 Covance 6329-212 GROUP AND DOSE LEVEL (PPM) 8 0 9 1 8 0 9 1 WEEK: 48 59/60 98% 58/60 97% 57/60 95% 60/60 100% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 49 59/60 98% 58/60 97% 57/60 95% 60/60 100% 50 59/60 98% 58/60 97% 56/60 93% 59/60 98% 51 58/60 97% 58/60 97% 56/60 93% 58/60 97% 52 53 MALE 58/60 97% 58/60 97% FEMALE 56/60 93% 58/60 97% 48/50 96% 48/50 96% 46/50 92% 48/50 96% 54 48/50 96% 48/50 96% 46/50 92% 48/50 96% 55 48/50 96% 48/50 96% 46/50 92% 48/50 96% 56 48/50 96% 48/50 96% 46/50 92% 48/50 96% 57 48/50 96% 48/50 96% 44/50 88% 48/50 96% Covance 6329-228 3M T-6316.1 PAGE: 5 58 59 48/50 96% 48/50 96% 48/5 96% 48/5 96% 43/50 86% 48/50 96% 43/5 86% 48/5 96% 3459 Covance 6329-212 GROUP AND DOSE LEVEL (PPM) 8 0 9 1 8 0 9 1 WEEK: 60 48/50 96% 47/50 94% 43/50 86% 48/50 96% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 61 48/50 96% 47/50 94% 43/50 86% 47/50 94% 62 48/50 96% 47/50 94% 43/50 86% 45/50 90% 63 48/50 96% 45/50 90% 41/50 82% 43/50 86% 64 65 MALE 47/50 94% 45/50 90% FEMALE 40/50 80% 43/50 86% 46/50 92% 44/50 88% 40/50 80% 43/50 86% 66 46/50 92% 44/50 88% 40/50 80% 43/50 86% 67 45/50 90% 43/50 86% 40/50 80% 43/50 86% 68 44/50 88% 42/50 84% 40/50 80% 41/50 82% 69 43/50 86% 42/50 84% 39/50 78% 41/50 82% Covance 6329-228 3M T-6316.1 PAGE: 6 70 71 42/50 84% 42/50 84% 41/5 82% 42/5 84% 38/50 76% 40/50 80% 38/5 76% 40/5 80% 3460 Covance 6329-212 GROUP AND DOSE LEVEL (PPM) 8 0 9 1 8 0 9 1 WEEK: 72 40/50 80% 42/50 84% 37/50 74% 40/50 80% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 73 40/50 80% 39/50 78% 36/50 72% 39/50 78% 74 40/50 80% 39/50 78% 35/50 70% 39/50 78% 75 40/50 80% 38/50 76% 35/50 70% 39/50 78% 76 77 MALE 38/50 76% 37/50 74% FEMALE 35/50 70% 39/50 78% 36/50 72% 37/50 74% 35/50 70% 38/50 76% 78 36/50 72% 37/50 74% 34/50 68% 38/50 76% 79 35/50 70% 37/50 74% 33/50 66% 38/50 76% 80 35/50 70% 36/50 72% 33/50 66% 37/50 74% 81 35/50 70% 36/50 72% 32/50 64% 35/50 70% Covance 6329-228 3M T-6316.1 PAGE: 7 82 83 34/50 68% 36/50 72% 33/50 66% 36/50 72% 30/50 60% 35/50 70% 29/50 58% 34/50 68% 3461 Covance 6329-212 GROUP AND DOSE LEVEL (PPM) 8 0 9 1 8 0 9 1 WEEK: 84 32/50 64% 36/50 72% 29/50 58% 34/50 68% Table 4 Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 85 30/50 60% 35/50 70% 28/50 56% 33/50 66% 86 28/50 56% 35/50 70% 28/50 56% 33/50 66% 87 26/50 52% 34/50 68% 27/50 54% 33/50 66% 88 89 MALE 26/50 52% 34/50 68% FEMALE 26/50 52% 33/50 66% 26/50 52% 33/50 66% 25/50 50% 31/50 62% 90 25/50 50% 32/50 64% 25/50 50% 31/50 62% 91 23/50 46% 32/50 64% 25/50 50% 30/50 60% 92 23/50 46% 32/50 64% 24/50 48% 28/50 56% 93 21/50 42% 31/50 62% 22/50 44% 28/50 56% Covance 6329-228 3M T-6316.1 PAGE: 8 94 95 21/50 42% 31/50 62% 20/5 40% 31/5 62% 19/50 38% 27/50 54% 19/5 38% 27/5 54% 3462 Table 4 Survival Data GROUP AND DOSE LEVEL (PPM) 8 0 9 1 8 0 9 1 WEEK: 96 20/50 40% 29/50 58% 17/50 34% 25/50 50% 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS 97 19/50 38% 26/50 52% 17/50 34% 24/50 48% 98 19/50 38% 26/50 52% 17/50 34% 23/50 46% 99 18/50 36% 26/50 52% 17/50 34% 21/50 42% 100 101 MALE 17/50 34% 25/50 50% FEMALE 16/50 32% 18/50 36% 15/50 30% 23/50 46% 15/50 30% 18/50 36% 102 14/50 28% 23/50 46% 14/50 28% 17/50 34% 103 13/50 26% 23/50 46% 14/50 28% 16/50 32% 104 11/5 22% 20/5 40% 13/5 26% 15/5 30% Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 9 3463 Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DAYS 1-730 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** APPEARANCE CONVULSIONS HEAD TILT LEFT RIGHT HUNCHED LIMITED USE LIMB-HIND-LEFT LIMBS-HIND MALOCCLUSION MISSING TEETH OBESE PARALYSIS LIMB-FRONT-LEFT PROTRUDING PENIS SWOLLEN HEAD-MAXILLARY-RIGHT MOUTH NOSE PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND THIN EXCESSIVE BLEEDING FROM PAWS, CAUTERI WITH SILVER NITRATE NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: --MALE- - -FEMALE8989 0101 NUMBER: 70 70 70 70 0100 1064 1116 4753 0101 1000 13 7 19 9 1100 2101 0100 2100 0010 0010 2000 3001 1 1 02 1001 22 17 17 13 000 3464 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 1 Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DAYS 1-730 CATEGORY KEYWORD QUALIFIER NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: -- MALE-- -FEMALE8989 0101 NUMBER: 70 70 70 70 BEHAVIOR AGGRESSIVE <UNSPECIFIED> MODERATE ATAXIC <UNSPECIFIED> MODERATE CIRCLING LEFT RIGHT HYPOACTIVE <UNSPECIFIED> MILD RECUMBENT LATERAL STERNAL SENSITIVE TO TOUCH ANIMAL IS CHEWING ON DISTAL PORTION OF TAIL COLD TO TOUCH ENTIRE BODY DISCHARGE GENITAL RED IN COLOR CLEAR ORAL <UNSPECIFIED> 0100 0100 2268 0010 0001 0010 16 10 10 13 0001 1001 02 00 0312 1000 3114 1301 0100 3465 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 2 Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DAYS 1-730 CATEGORY KEYWORD QUALIFIER NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE : -MALE- -FEMALE8989 0101 NUMBER: 70 70 70 70 DISCHARGE CLEAR ORAL MODERATE NASAL RED IN COLOR RED DISCHARGE RED ORAL MUCOID DISCHARGE FROM RECTUM EXCRETION FEW FECES NO FECES NONFORMED FECES EYES CLEAR DISCHARGE EYE-LEFT DISPLACED PUPIL EYE-RIGHT DILATED PUPIL EYES DISCOLORED/LIGHT RED EYES OPAQUE EYES PALE EYE-RIGHT EYES 0100 6196 1000 0010 0010 22 11 20 18 10 7 8 7 15 14 1 6 0010 0010 1000 0010 0100 0100 4112 3466 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 3 DAYS 1-730 CATEGORY KEYWORD QUALIFIER EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SCAB EYE-LEFT SQUINTED PERI-ORBITAL PERI-ORBITAL-LEFT PERI-ORBITAL-RIGHT RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE ALOPECIA BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL VENTRAL-CERVICAL GENERALIZED BLUE SKIN VENTRAL-ABDOMINAL-LEFT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE : -- MALE-- -FEMALE8989 0101 NUMBER: 70 70 70 70 3366 1121 6 2 10 11 0100 7434 2 001 0010 312 0 532 0 5232 0110 32 06 4321 1000 0010 0111 3467 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 4 DAYS 1-730 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL VENTRAL-THORACIC-LEFT PALE ENTIRE BODY RED HAIR COAT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-RIGHT HEAD-ENTIRE HEAD-CRANIAL PERINEAL AREA SHOULDER-LEFT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-CERVICAL ROUGH HAIR COAT RED SKIN TAIL SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-RIGHT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE : -MALE- -FEMALE8989 0101 NUMBER: 70 70 70 70 012 0 0010 4433 0101 0100 0100 6125 0022 2 01 0 0001 2 031 0001 0010 4 32 0 1000 002 0 0031 2 3 02 12 00 1000 3468 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 5 DAYS 1-730 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-LEFT LIMB-HIND-LEFT LUMBAR-RIGHT MOUTH PAW-FRONT-LEFT PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND PERINEAL AREA SCROTUM SCROTUM-RIGHT SHOULDER-RIGHT TAIL TAIL-DISTAL TAIL-PROXIMAL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE : -MALE- -FEMALE8989 0101 NUMBER: 70 70 70 70 1100 1000 02 1 0 0001 0010 0101 1001 0001 0100 1000 012 0 1000 74 01 6313 1211 0001 1000 0100 0010 0001 1000 1001 4411 2101 3469 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 6 DAYS 1-730 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT VENTRAL-THORACIC VENTRAL-THORACIC-RIGHT WARM TO TOUCH ENTIRE BODY YELLOW HAIR COAT *** END OF LIST *** Table 5 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE : --MALE- -FEMALE8989 0101 NUMBER: 70 70 70 70 1121 0112 0011 0100 0100 1100 0010 5375 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 7 3470 DAYS 1-730 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** MASS(ES) AXILLARY REGION-LEFT WEEK 42 WEEK 45 WEEK 48 WEEK 51 WEEK 59 WEEK 61 WEEK 74 WEEK 76 WEEK 77 WEEK 79 WEEK 80 WEEK 84 WEEK 92 WEEK 96 WEEK 101 WEEK 102 AXILLARY REGION-RIGHT WEEK 28 WEEK 39 WEEK 40 WEEK 42 WEEK 55 WEEK 57 WEEK 59 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE : 1 CO o MALE- -FEMALE989 101 NUMBER: 70 70 70 70 0001 0010 0001 0002 0010 0010 0001 002 0 0010 0001 0010 0001 0010 0 02 1 0001 0010 0001 0001 0010 0011 0001 0010 0011 3471 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 1 DAYS 1-730 CATEGORY KEYWORD QUALIFIER MASS(ES) AXILLARY REGION-RIGHT WEEK 60 WEEK 70 WEEK 73 WEEK 74 WEEK 75 WEEK 79 WEEK 80 WEEK 83 WEEK 84 WEEK 88 WEEK 91 WEEK 92 WEEK 96 WEEK 101 DORSAL WEEK 72 WEEK 83 WEEK 90 WEEK 95 HEAD-MAXILLARY-LEFT WEEK 93 WEEK 100 HEAD-MAXILLARY-RIGHT WEEK 66 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: -- MALE-- -FEMALE8989 0101 NUMBER: 70 70 70 70 002 0 0001 0001 0001 0010 0010 0010 0010 0010 0001 0010 0010 0002 0011 0100 0001 0100 1000 0100 0100 0001 3472 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 2 DAYS 1-730 CATEGORY KEYWORD QUALIFIER MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 74 INGUINAL REGION-LEFT WEEK 48 WEEK 64 WEEK 78 WEEK 79 WEEK 80 WEEK 84 WEEK 92 WEEK 95 WEEK 101 INGUINAL REGION-RIGHT WEEK 35 WEEK 40 WEEK 42 WEEK 56 WEEK 76 WEEK 84 WEEK 92 LIMB-FRONT-RIGHT WEEK 35 PAW-HIND-RIGHT WEEK 42 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: -- MALE-- -FEMALE8989 0101 NUMBER: 70 70 70 70 0100 0001 1000 0100 0010 0002 0001 0011 0001 0010 0001 0001 0001 0010 0010 0012 0002 0001 0001 3473 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 3 DAYS 1-730 CATEGORY KEYWORD QUALIFIER MASS(ES) SHOULDER-LEFT WEEK 38 SHOULDER-RIGHT WEEK 19 WEEK 55 TAIL-PROXIMAL WEEK 75 VENTRAL-ABDOMINAL WEEK 32 WEEK 35 WEEK 40 WEEK 44 WEEK 45 WEEK 50 WEEK 56 WEEK 61 WEEK 62 WEEK 74 WEEK 75 WEEK 76 WEEK 78 WEEK 79 WEEK 80 WEEK 84 WEEK 100 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: -- MALE-- -FEMALE8989 0101 NUMBER: 70 70 70 70 0010 0010 0001 0001 1000 0001 0100 0001 0100 0011 0001 0001 0001 1010 1001 0100 1000 0100 002 0 0100 002 0 3474 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 4 DAYS 1-730 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL -ABDOMINAL WEEK 102 WEEK 105 VENTRAL -ABDOMINAL-LEFT WEEK 28 WEEK 34 WEEK 40 WEEK 44 WEEK 46 WEEK 49 WEEK 50 WEEK 51 WEEK 61 WEEK 66 WEEK 69 WEEK 72 WEEK 73 WEEK 75 WEEK 76 WEEK 100 WEEK 101 WEEK 102 WEEK 103 VENTRAL -ABDOMINAL-RIGHT WEEK 28 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: 1 CO o MALE- -FEMALE989 101 NUMBER: 70 70 70 70 0001 0100 1000 0001 1000 0 02 1 0100 002 0 0 02 1 002 0 0011 0002 0010 0100 0030 0001 0111 0010 0001 0010 0001 1000 3475 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 5 DAYS 1-730 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL -ABDOMINAL-RIGHT WEEK 33 WEEK 40 WEEK 44 WEEK 45 WEEK 48 WEEK 51 WEEK 53 WEEK 61 WEEK 63 WEEK 64 WEEK 66 WEEK 70 WEEK 74 WEEK 76 WEEK 78 WEEK 79 WEEK 80 WEEK 84 WEEK 92 WEEK 101 VENTRAL -CERVICAL WEEK 36 WEEK 46 WEEK 54 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: 1 CO o MALE- -FEMALE989 101 NUMBER: 70 70 70 70 1000 0001 0001 0001 0100 0010 002 0 0001 1000 0001 0010 0001 0001 0100 1000 1001 0001 02 1 0 0001 0001 0010 002 0 0001 3476 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 6 DAYS 1-730 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL-CERVICAL WEEK 65 WEEK 75 WEEK 77 WEEK 80 WEEK 84 WEEK 88 VENTRAL-CERVICAL-LEFT WEEK 31 WEEK 38 WEEK 70 WEEK 95 VENTRAL-THORACIC WEEK 70 WEEK 80 VENTRAL-THORACIC-LEFT WEEK 65 WEEK 74 VENTRAL-THORACIC-RIGHT WEEK 82 WART LIKE LESION(S) DORSAL WEEK 41 WEEK 59 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: -- MALE-- -FEMALE8989 0101 NUMBER: 70 70 70 70 0010 0001 0001 0001 0001 0001 0010 0001 0001 0010 0010 0010 0001 0010 0100 1000 0100 3477 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 7 DAYS 1-730 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) DORSAL WEEK 62 WEEK 72 WEEK 76 WEEK 82 WEEK 93 EAR-LEFT WEEK 92 HEAD-MAXILLARY-RIGHT WEEK 64 WEEK 79 LIMB-FRONT-RIGHT WEEK 105 MOUTH WEEK 54 WEEK 100 NOSE WEEK 46 WEEK 68 WEEK 100 TAIL-MID WEEK 101 TAIL-PROXIMAL WEEK 64 WEEK 74 Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: -- MALE-- -FEMALE8989 0101 NUMBER: 70 70 70 70 0100 0100 0100 0100 1000 0010 1000 0001 0100 0010 0100 0010 1000 0100 0100 0100 1000 3478 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 8 DAYS 1-730 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 20 WEEK 58 WEEK 92 VENTRAL-ABDOMINAL-RIGHT WEEK 82 VENTRAL-THORACIC-RIGHT WEEK 82 WEEK 101 f* END OF LIST *** Table 6 Summary of Mass Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP : DOSE: --MALE-- -FEMALE8989 0101 NUMBER: 70 70 70 70 1000 0100 0100 0100 0100 0100 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 9 3479 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 1N 70 MEAN 204 S.D. 18.8 2N 70 MEAN 260 S.D. 21.9 3N 70 MEAN 309 S.D. 25.3 4N 70 MEAN 351 S.D. 28.4 5N 65 MEAN 381 S.D. 33.1 6N 65 MEAN 409 S.D. 36.9 7N 65 MEAN 433 S.D. 40.2 70 202 19.4 70 260 22.1 70 309 25.4 70 350 28.4 65 383 33.4 65 413 36.4 65 438 40.4 70 152 10.8 70 179 13.9 70 202 16.1 70 224 19.6 65 240 19.9 65 253 22.3 65 263 24.5 70 152 11 70 179 13 70 203 15 70 223 17 65 236 17 65 251 20 65 260 21 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 1 3480 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE-------- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 8N 65 MEAN 451 S.D. 43.0 9N 65 MEAN 468 S.D. 45.9 10 N 65 MEAN 484 S.D. 49.7 11 N 65 MEAN 496 S.D. 54.3 12 N 64 MEAN 510 S.D. 59.2 13 N 64 MEAN 523 S.D. 58.0 14 N 64 MEAN 531 S.D. 58.7 65 454 42.9 65 472 45.3 65 490 47.4 65 503 48.9 65 517 51.2 65 529 53.4 65 537 54.4 64 272 25.2 64 282 27.6 64 287 28.4 64 287 29.0 64 296 30.2 64 301 30.7 64 306 31.4 64 267 23 65 274 23 65 281 25 65 284 26 65 288 27 65 294 27 65 298 29 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 2 3481 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 15 N 59 MEAN 541 S.D. 60.2 16 N 59 MEAN 553 S.D. 60.8 17 N 59 MEAN 562 S.D. 61.8 21 N 59 MEAN 591 S.D. 68.4 25 N 59 MEAN 617 S.D. 73.3 29 N 59 MEAN 631 S.D. 76.8 33 N 59 MEAN 652 S.D. 81.0 60 545 56.4 60 555 59.9 60 564 62.7 60 593 70.0 60 616 74.5 60 630 77.5 60 655 84.4 59 307 34.1 59 314 35.0 59 319 35.8 59 332 41.3 59 345 46.7 59 354 48.3 58 371 53.9 60 302 30 60 307 32 60 309 33 60 325 39 60 340 46 60 348 51 60 366 60 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 3 3482 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 37 N 59 MEAN 681 S.D. 85.7 41 N 59 MEAN 697 S.D. 90.0 45 N 59 MEAN 719 S.D. 94.9 49 N 59 MEAN 732 S.D. 98.6 53 N 58 MEAN 751 S.D. 102.7 57 N 48 MEAN 759 S.D. 107.8 61 N 48 MEAN 771 S.D. 116.1 60 674 89.2 60 693 93.2 58 709 99.8 58 726 105.2 58 738 110.7 48 741 123.5 47 748 138.9 58 389 60.1 58 402 63.8 58 416 68.8 57 431 74.4 56 445 78.1 45 452 87.7 43 474 94.0 60 380 60 60 392 64 60 407 68 60 423 72 58 436 74 48 456 87 48 474 95 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 4 3483 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE-------- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 65 N 47 MEAN 776 S.D. 116.3 69 N 44 MEAN 768 S.D. 135.9 73 N 40 MEAN 791 S.D. 140.6 77 N 38 MEAN 791 S.D. 154.4 81 N 35 MEAN 797 S.D. 146.7 85 N 32 MEAN 786 S.D. 162.7 89 N 26 MEAN 795 S.D. 158.6 45 761 143.0 42 779 131.8 42 773 142.6 37 773 126.5 36 788 127.3 36 787 132.7 34 772 144.2 40 492 91.2 40 498 97.9 37 511 98.6 35 522 96.8 33 516 102.6 29 531 97.3 26 532 97.6 43 481 102 41 485 102 40 499 103 39 498 106 37 497 111 34 510 120 33 511 128 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 5 3484 Table 7 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP: DOSE: WEEK UNITS: --MALE-------89 01 PPM PPM -FEMALE--8 0 PPM PPM 93 N MEAN S.D. 97 N MEAN S.D. 101 N MEAN S.D. 105 N MEAN S.D. 23 791 157.9 20 786 119.5 17 710 148.5 11 656 206.2 32 771 140.7 28 758 153.9 25 727 144.4 20 739 127.5 24 518 110.6 17 542 102.5 16 517 108.3 13 515 126.2 28 519 133 25 504 111 18 517 77 15 515 69 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 6 3485 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE- GROUP: 8 DOSE: 0 WEEK UNITS: PPM 9 1 PPM ------- FEMALE 8 0 PPM 9 1 PPM 1-15 N MEAN S.D. 15-29 N MEAN S.D. 29-57 N MEAN S.D. 57-105 N MEAN S.D. 1-105 N MEAN S.D. 59 337 50.0 59 90 23.0 48 129 49.3 11 -70 194.7 11 455 200.2 60 342 47.4 60 85 28.0 48 116 53.5 20 38 78.3 20 547 124.1 59 155 28.3 59 47 21.9 45 96 54.4 13 94 89.1 13 366 120.9 60 149 25.5 60 46 26.9 48 107 47.6 15 99 37.2 15 364 67.3 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 1 3486 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 1N 70 MEAN 167 S.D. 12.9 2N 69 MEAN 174 S.D. 15.6 3N 69 MEAN 180 S.D. 15.6 4N 63 MEAN 182 S.D. 16.2 5N 63 MEAN 185 S.D. 17.7 6N 62 MEAN 186 S.D. 19.2 7N 65 MEAN 189 S.D. 19.1 69 166 13.9 69 175 17.4 67 180 21.6 64 183 17.7 60 186 19.2 62 187 19.5 64 189 20.1 46 128 13.5 51 129 13.9 63 137 19.3 50 138 13.6 55 136 14.5 55 141 18.8 61 141 17.8 57 126 12 55 129 13 66 138 20 58 138 18 53 137 12 52 139 13 57 137 15 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 1 3487 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 8N 63 MEAN 188 S.D. 19.6 9N 63 MEAN 188 S.D. 18.9 10 N 63 MEAN 186 S.D. 28.8 11 N 62 MEAN 187 S.D. 19.9 12 N 63 MEAN 192 S.D. 19.4 13 N 64 MEAN 194 S.D. 19.7 14 N 47 MEAN 196 S.D. 19.0 61 188 20.8 64 191 21.0 63 190 17.6 61 187 16.9 64 194 17.6 64 195 18.4 50 189 16.2 54 139 17.4 57 137 18.4 51 138 15.1 52 132 14.9 53 131 16.7 60 136 17.6 42 131 13.7 48 137 17 58 135 16 51 136 20 51 127 13 55 134 13 61 140 19 38 138 20 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 2 3488 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 15 N 59 MEAN 190 S.D. 19.5 16 N 55 MEAN 192 S.D. 20.3 20 N 58 MEAN 187 S.D. 19.9 24 N 58 MEAN 191 S.D. 21.3 28 N 55 MEAN 194 S.D. 20.0 32 N 59 MEAN 192 S.D. 21.3 36 N 58 MEAN 190 S.D. 18.5 58 193 18.4 60 193 20.6 57 187 19.6 60 190 17.9 56 194 18.0 60 197 20.7 56 192 19.4 56 132 14.8 47 131 18.2 56 136 19.3 57 144 28.2 53 150 21.4 57 149 21.9 57 144 19.1 54 138 22 48 134 14 52 141 24 55 141 20 57 151 23 59 151 23 54 148 15 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 3 3489 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 40 N 59 MEAN 189 S.D. 19.9 44 N 58 MEAN 185 S.D. 21.8 48 N 59 MEAN 182 S.D. 21.8 52 N 58 MEAN 189 S.D. 21.7 56 N 45 MEAN 182 S.D. 22.0 60 N 46 MEAN 188 S.D. 29.9 64 N 47 MEAN 188 S.D. 36.6 60 189 19.6 58 186 21.4 58 187 21.4 57 183 20.7 47 179 28.4 45 185 33.4 44 188 34.1 57 143 17.7 58 143 17.3 56 144 19.9 55 146 27.1 44 143 34.3 39 151 26.7 38 157 26.5 57 144 20 58 145 18 59 146 27 51 148 27 43 144 22 44 149 31 41 148 39 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 4 3490 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE 89 01 PPM PPM 68 N 43 MEAN 184 S.D. 42.8 72 N 38 MEAN 195 S.D. 34.3 76 N 37 MEAN 191 S.D. 43.2 80 N 34 MEAN 177 S.D. 36.4 84 N 32 MEAN 163 S.D. 51.1 N MEAN S.D. 26 169 40.0 92 N 22 MEAN 166 S.D. 43.5 41 195 26.5 41 189 40.9 37 193 24.7 33 181 19.5 34 176 21.4 32 173 27.0 31 167 31.6 39 161 37.5 36 165 40.8 28 160 27.4 28 139 23.4 26 133 39.1 25 141 27.0 21 129 40.7 41 158 26 39 158 25 36 156 29 34 132 40 32 138 36 33 137 36 28 127 34 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 5 CO CO 3491 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: GROUP: DOSE: WEEK UNITS: -MALE-------89 01 PPM PPM -FEMALE--8 0 PPM PPM 96 N MEAN S.D. 100 N MEAN S.D. 104 N MEAN S.D. 20 158 41.0 14 141 65.2 8 176 44.6 27 157 42.0 24 173 29.0 20 167 35.5 17 146 20.2 15 140 32.4 12 141 32.0 23 124 48 14 139 31 12 144 28 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 6 3492 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ---MALE--- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM 1N 69 57 MEAN 0.103 0.108 S.D. 0.007 0.010 2N 69 55 MEAN 0.088 0.096 S.D. 0.007 0.009 3N MEAN S.D. 67 0.078 0.008 66 0.092 0.013 4N 64 58 MEAN 0.071 0.086 S.D. 0.004 0.011 5N MEAN S.D. 60 0.066 0.004 53 0.080 0.005 6N MEAN S.D. 62 0.063 0.003 52 0.077 0.007 7N 64 56 MEAN 0.061 0.075 S.D. 0.005 0.008 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 1 3493 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ---MALE--- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM 8N MEAN S.D. 9N MEAN S.D. 10 N MEAN S.D. 11 N MEAN S.D. 12 N MEAN S.D. 13 N MEAN S.D. 14 N MEAN S.D. 61 0.058 0.005 64 0.057 0.004 63 0.055 0.004 61 0.053 0.003 64 0.053 0.004 64 0.052 0.003 50 0.051 0.003 47 0.072 0.010 58 0.070 0.009 51 0.069 0.010 51 0.063 0.007 55 0.066 0.006 61 0.067 0.009 38 0.065 0.009 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 2 3494 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ---MALE--- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM 15 N MEAN S.D. 16 N MEAN S.D. 20 N MEAN S.D. 24 N MEAN S.D. 28 N MEAN S.D. 32 N MEAN S.D. 36 N MEAN S.D. 58 0.050 0.003 60 0.049 0.003 57 0.045 0.003 60 0.045 0.004 56 0.044 0.004 60 0.043 0.003 56 0.041 0.003 54 0.064 0.012 48 0.062 0.006 52 0.062 0.012 55 0.060 0.009 57 0.062 0.008 59 0.060 0.010 54 0.056 0.007 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 3 3495 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ---MALE--- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM 40 N MEAN S.D. 44 N MEAN S.D. 48 N MEAN S.D. 52 N MEAN S.D. 56 N MEAN S.D. 60 N MEAN S.D. 64 N MEAN S.D. 60 0.039 0.003 58 0.038 0.003 58 0.037 0.003 57 0.036 0.003 47 0.035 0.005 45 0.035 0.004 44 0.035 0.005 57 0.053 0.007 58 0.052 0.006 59 0.050 0.009 51 0.049 0.008 43 0.046 0.005 44 0.045 0.008 41 0.044 0.012 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 4 3496 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: ---MALE--- ---FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM 68 N MEAN S.D. 72 N MEAN S.D. 76 N MEAN S.D. 80 N MEAN S.D. 84 N MEAN S.D. N MEAN S.D. 92 N MEAN S.D. 41 0.036 0.005 41 0.035 0.006 37 0.036 0.005 33 0.033 0.004 34 0.032 0.004 32 0.032 0.004 31 0.031 0.007 41 0.047 0.007 39 0.046 0.007 36 0.046 0.008 34 0.039 0.011 32 0.039 0.009 33 0.039 0.009 28 0.036 0.010 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 5 CO CO 3497 Table 10 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: -- MALE--- -- FEMALE-- GROUP: 9 9 DOSE: 1 1 WEEK UNITS: PPM PPM 96 N MEAN S.D. 100 N MEAN S.D. 104 N MEAN S.D. 27 0.029 0.007 24 0.034 0.003 20 0.032 0.004 23 0.034 0.013 14 0.039 0.012 12 0.040 0.007 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 6 3498 Table 11 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/iL G/DL % FL PG % X103/iL 0 MEAN S.D. N 1 MEAN S.D. N 8.32 .838 10 8.38 .411 10 15.2 1.18 10 15.0 .64 10 44.3 3.23 10 44.1 1.62 10 53.4 2.55 10 52.6 1.35 10 18.3 .80 10 17.9 .53 10 34.2 .45 10 34.0 .31 10 1094 94.6 10 1094 90.4 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3499 Covance 6329-212 DOSE ppm WBC X103/^L Table 11 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/^L X103/m L MONO EOSIN X103/^L X103/m L BASO N-SEG% X103/^L LYMPH% MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% 0 MEAN 10.4 1.3 7.9 .9 .3 .0 13 76 93 0 S.D. 2.18 .28 2.04 .23 .12 .00 2.6 5.2 2.9 1.3 .0 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 10.2 1.3 7.5 1.1 .3 .0 13 74 11 2 0 S.D. 2.12 .39 1.72 .50 .18 .03 4.1 4.4 3.9 1.3 .0 N 10 10 10 10 10 10 10 10 10 10 10 3500 Table 11 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/iL G/DL % FL PG % X103/iL 0 MEAN S.D. N 1 MEAN S.D. N 7.82 .358 10 8.01 .350 10 14.7 .73 10 15.2 .39 10 43.1 2.10 10 44.2 1.44 10 55.1 1.05 10 55.3 1.32 10 18.8 .53 10 19.0 .53 10 34.2 .45 10 34.4 .52 10 1064 88 10 1112 83 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3501 Covance 6329-212 DOSE ppm WBC X103/^L Table 11 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/^L X103/m L MONO EOSIN BASO X103/^L X103/m L X103/m L N-SEG! LYMPH MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% 0 MEAN 7.3 .8 5.8 .5 .2 .0 12 78 7 3 0 S.D. 2.61 .38 2.24 .16 .09 .00 4.3 4.7 1.7 .9 .0 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 6.5 .9 5.0 .4 .2 .0 15 75 7 3 0 S.D. 1.23 .20 1.37 .17 .06 .00 5.0 8.7 3.7 1.0 .0 N 10 10 10 10 10 10 10 10 10 10 10 3502 Table 12 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/m L G/DL % FL PG % X103/^L 0 MEAN S.D. N 1 MEAN S.D. N 8.22 .430 10 8.28 .487 10 15.2 .67 10 14.8 .78 10 44.6 1.98 10 43.9 2.19 10 54.3 1.63 10 53.1 1.28 10 18.5 .62 10 34.0 .36 10 17.9 * .54 10 33.8 .39 10 1083 109 10 1112 83 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3503 Covance 6329-212 DOSE ppm WBC X103/^L Table 12 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/^L X103/m L MONO EOSIN BASO X103/^L X103/m L X103/m L N-SEG! LYMPH MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% 0 MEAN 8.6 1.7 5.7 1.0 .2 .0 19 66 11 3 0 S.D. 1.85 .81 1.12 .34 .13 .04 5.4 8.0 4.0 1.6 .5 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 8.9 1.5 5.9 1.2 .2 .1 17 66 14 2 1 S.D. 1.34 .28 1.17 .67 .08 .13 4.0 7.6 6.2 1.0 .9 N 10 10 10 10 10 10 10 10 10 10 10 3504 Table 12 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/iL G/DL % FL PG % X103/iL 0 MEAN S.D. N 1 MEAN S.D. N 7.31 .267 10 7.63 .451 10 13.9 .75 10 40.6 1.79 10 55.6 1.46 10 14.7 * .54 10 42.5 * 1.93 10 55.8 1.45 10 19.1 .66 10 19.3 .58 10 34.3 .50 10 34.6 .41 10 1038 101.1 10 1098 161.0 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3505 Covance 6329-212 DOSE ppm WBC X103/^L Table 12 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/^L X103/m L MONO EOSIN BASO X103/^L X103/m L X103/^L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% 0 MEAN 6.4 1.0 4.6 .6 .2 .0 15 73 92 0 S.D. 2.32 .72 1.46 .28 .15 .00 5.7 7.7 2.3 1.2 .5 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 5.8 .9 4.2 .5 .1 .0 16 72 92 0 S.D. 1.90 .30 1.63 .21 .04 .00 5.8 9.9 3.6 .8 .3 N 10 10 10 10 10 10 10 10 10 10 10 3506 Table 13 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/iL G/DL % FL PG % X103/iL 0 MEAN S.D. N 1 MEAN S.D. N 8.20 .530 10 8.42 .439 10 15.2 .78 10 15.2 .71 10 42.6 2.22 10 43.4 1.97 10 52.0 1.89 10 51.6 2.54 10 18.5 .69 10 18.1 1.04 10 35.6 .24 10 1171 158 10 35.2 * .38 10 1131 185 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3507 Covance 6329-212 DOSE ppm WBC X103/^L Table 13 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/m L MONO EOSIN BASO X103/m L X103/m L X103/m L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% 0 MEAN 6.8 2.0 3.9 .8 .1 .0 29 57 11 2 0 S.D. 1.64 .73 1.29 .34 .05 .03 8.6 10.4 4.7 .9 .4 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 6.7 2.0 3.8 .8 .1 .0 28 57 12 2 0 S.D. 2.12 1.17 .93 .29 .05 .00 7.3 6.7 2.7 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 3508 Table 13 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X106/iL G/DL % FL PG % X103/iL 0 MEAN S.D. N 1 MEAN S.D. N 7.71 .362 10 7.78 .435 10 14.9 .58 10 14.9 .46 10 41.4 1.77 10 41.6 1.16 10 53.8 1.63 10 53.5 1.75 10 19.4 .67 10 19.2 .61 10 36.0 .46 10 35.7 .41 10 941 117.1 10 1053 * 102.5 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3509 Covance 6329-212 DOSE ppm WBC X103/m L Table 13 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103/m L X103/m L MONO EOSIN BASO X103/m L X103/m L X103/m L N-SEG! LYMPH: MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% 0 MEAN 5.2 1.4 3.2 .6 .1 .0 27 60 11 2 0 S.D. 1.64 .48 1.16 .13 .03 .00 4.7 5.8 2.3 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 5.1 1.3 3.2 .6 .1 .0 26 60 12 2 0 S.D. 2.18 .73 1.66 .24 .04 .03 8.6 9.5 4.6 .7 .4 N 10 10 10 10 10 10 10 10 10 10 10 3510 Table 14 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 1 MEAN S.D. N 7 2.6 5 7 3.8 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 3511 Table 14 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 1 MEAN S.D. N 3 1.1 5 4 2.3 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 3512 DOSE ppm Table 15 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 113 14 .7 6.9 4.8 2.1 .0 59 S.D. 9.6 2.1 .10 .40 .22 .28 .05 7.0 N 10 10 10 10 10 10 10 10 1 MEAN 114 13 .6 7.0 4.7 2.3 .0 63 S.D. 7.4 2.1 .11 .32 .19 .26 .05 12.8 N 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3513 Covance 6329-212 DOSE ppm Table 15 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 1 MEAN S.D. N 110 21.4 10 111 14.9 10 39 8.1 10 48 21.2 10 2 2.1 10 3 2.2 10 10.6 .19 10 10.6 .24 10 7.3 .63 10 7.0 .31 10 147 .8 10 147 .8 10 5.9 .39 10 6.3 * .31 10 106 1.9 10 107 .6 10 Covance 6329-228 3M T-6316.1 3514 DOSE ppm Table 15 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 114 14 .8 8.2 6.1 2.1 .2 94 S.D. 8.7 2.3 .14 .60 .52 .22 .16 22.1 N 10 10 10 10 10 10 10 10 1 MEAN 110 15 .7 7.9 5.8 2.1 .1 88 S.D. 3.8 2.4 .12 .68 .66 .27 .13 20.3 N 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3515 DOSE ppm Table 15 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 1 MEAN S.D. N 110 58.6 10 108 24.1 10 44 24.9 10 44 19.1 10 2 1.3 10 2 2.0 10 11.4 .54 10 11.2 .29 10 6.0 .44 10 6.4 * .43 10 145 1.6 10 147 * .8 10 5.8 .38 10 6.0 .36 10 106 1.8 10 107 2.2 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3516 Table 15 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 1 MEAN S.D. N 6 1.3 5 5 1.1 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 3517 Table 15 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 1 MEAN S.D. N 3 .9 5 5 1.6 5 Covance 6329-212 Covance 6329-228 3M T-6316.1 3518 DOSE ppm Table 16 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 103 12 .6 6.9 4.6 2.2 .1 75 S.D. 10.1 1.2 .06 .32 .17 .28 .05 12.5 N 10 10 10 10 10 10 10 10 1 MEAN 111 * 13 .6 7.1 4.6 2.5 .2 84 S.D. 7.3 .9 .04 .29 .18 .25 .05 17.1 N 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3519 DOSE ppm Table 16 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 1 MEAN S.D. N 120 21.8 10 110 20.4 10 43 23.3 10 45 18.1 10 2 .8 10 1 .8 10 10.6 .16 10 10.8 * .20 10 7.0 .41 10 7.0 .47 10 146 1.2 10 147 1.1 10 5.9 .34 10 6.3 * .29 10 105 2.0 10 105 2.6 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3520 DOSE ppm Table 16 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN S.D. N 1 MEAN S.D. N 106 8.0 10 104 7.2 10 14 1.7 10 13 2.2 10 .7 .03 10 .7 * .05 10 8.3 .41 10 8.2 .45 10 6.1 .32 10 5.9 .64 10 2.2 .23 10 2.3 .50 10 .2 117 .00 27.5 10 10 .2 111 .06 33.8 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3521 DOSE ppm Table 16 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 1 MEAN S.D. N 115 50.2 10 178 168.9 10 58 38.8 10 96 113.8 10 2 1.9 10 4 6.3 10 11.3 .38 10 11.2 .43 10 5.6 .59 10 6.1 .59 10 144 1.3 10 146 * 1.3 10 5.2 .50 10 5.5 .54 10 103 2.7 10 104 2.3 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3522 DOSE ppm Table 17 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 119 12 .7 7.1 4.6 2.5 S.D. 19.5 2.0 .09 .35 .37 .29 N 10 10 10 10 10 10 1 MEAN 127 12 .7 7.1 4.6 2.5 S.D. 13.2 2.3 .03 .31 .31 .30 N 10 10 10 10 10 10 .2 105 .05 33.8 10 10 .2 102 .05 42.1 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3523 DOSE ppm Table 17 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 1 MEAN S.D. N 108 55.4 10 110 40.0 10 77 118.8 10 42 12.3 10 2 1.1 10 1 1.3 10 10.3 .55 10 10.5 .34 10 6.2 .89 10 6.4 .53 10 149 3.5 10 150 4.3 10 6.1 .58 10 6.4 .50 10 106 3.0 10 107 3.8 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3524 DOSE ppm Table 17 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN S.D. N 1 MEAN S.D. N 113 17.5 10 108 8.9 10 11 1.1 10 14 * 2.5 10 .8 .07 10 .8 .07 10 8.4 .44 10 8.5 .47 10 5.9 .36 10 5.8 .40 10 2.4 .34 10 2.7 .41 10 .3 142 .22 34.0 10 10 .2 126 .06 20.8 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3525 DOSE ppm Table 17 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 1 MEAN S.D. N 100 29.1 10 156 160.1 10 54 24.7 10 72 62.1 10 2 1.0 10 2 2.6 10 11.1 .44 10 11.0 .50 10 5.4 1.05 10 5.7 1.14 10 147 4.1 10 147 2.3 10 5.4 .37 10 5.3 .68 10 102 2.6 10 104 3.0 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3526 Table 18 Summary of Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN 22.7 1.015 S.D. 15.31 .0116 N 10 10 1 MEAN 21.9 1.017 S.D. 13.22 .0085 N 10 10 7.1 .32 10 7.2 .24 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3527 Table 18 Summary of Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN 12.5 1.017 S.D. 13.88 .0078 N 10 10 1 MEAN 22.0 1.012 S.D. 18.74 .0112 N 10 10 6.8 .26 10 6.7 .26 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3528 Table 19 Summary of Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN 13.8 1.022 S.D. 12.00 .0081 N 10 10 1 MEAN 15.4 1.026 S.D. 13.27 .0125 N 10 10 6.9 .39 10 7.0 .28 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3529 Table 19 Summary of Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN 17.1 1.019 S.D. 17.79 .0134 N 10 10 1 MEAN 20.0 1.018 S.D. 17.04 .0126 N 10 10 6.6 .21 10 6.6 .16 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3530 Table 20 Summary of Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN 14.7 1.026 S.D. 12.83 .0110 N 10 10 1 MEAN 10.5 1.030 S.D. 6.47 .0123 N 10 10 7.0 .33 10 6.9 .21 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3531 Table 20 Summary of Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN 27.2 1.014 S.D. 19.88 .0106 N 10 10 1 MEAN 13.0 1.019 S.D. 10.19 .0093 N 10 10 6.5 .24 10 6.5 .33 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3532 Table 21 Summary of Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 24 16.7 10 30 14.3 10 80 72.9 10 89 43.9 10 .35 .079 9 1.06 .227 9 .51 * .151 9 1.53 * .425 9 Covance 6329-212 Covance 6329-228 3M T-6316.1 3533 Table 21 Summary of Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 26 12.0 10 18 12.0 10 76 34.6 10 51 41.3 10 .22 .119 9 .22 .126 8 .64 .273 9 .56 .333 8 Covance 6329-212 Covance 6329-228 3M T-6316.1 3534 Table 22 Summary of Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 18 9.3 10 26 18.7 10 112 52.8 10 151 123.3 10 .18 .062 10 .24 .135 9 1.11 .383 10 1.34 .363 9 Covance 6329-212 Covance 6329-228 3M T-6316.1 3535 Table 22 Summary of Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 16 16.3 10 10 7.1 9 101 83.6 10 70 47.4 9 .08 .025 8 .14 * .057 9 .61 .212 8 .96 * .383 9 Covance 6329-212 Covance 6329-228 3M T-6316.1 3536 Table 23 Summary of Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 20 13.0 10 26 17.9 10 143 68.2 10 165 88.6 10 .23 .135 9 .20 .100 10 1.61 .579 9 1.29 .256 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3537 Table 23 Summary of Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 13 9.6 10 20 14.9 10 64 48.6 10 118 101.1 10 .25 .191 6 .18 .085 10 .93 .569 6 .93 .290 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 3538 Covance 6329-212 Table 24 Summary of Organ Weight Data Week 4 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=1;SUBSET=T LIVER SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 314.0 18.3 5 11.5023 1.4141 ORGAN-TOBODY WT (%) 5 3.6573 0.3062 ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 199.2 14.9 ORGAN WEIGHT (g) 5 7.7008 0.9577 Covance 6329-228 3M T-6316.1 PAGE: 1 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 3.8600 0.2780 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 308.7 14.1 5 11.1683 0.9088 5 3.6223 0.3092 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 218.0 20.1 5 8.1095 1.1330 5 3.7211 0.3797 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 O 1 1 1 1 1 &-i 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 O 1 1 1 1 11 2 3539 Covance 6329-212 Table 25 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=T LIVER SEX DOSE GROUP M8 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 501.8 59.4 5 14.4950 2.0800 ORGAN-TOBODY WT (%) 5 2.8829 0.1069 ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 270.7 16.9 ORGAN WEIGHT (g) 5 8.0939 0.4382 Covance 6329-228 3M T-6316.1 PAGE: 1 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 3.0001 0.2600 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 525.2 47.7 4 16.6952 2.7892 4 3.1146 0.2426 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 254.9 23.0 5 7.5958 0.1717 5 2.9983 0.2565 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 O 1 1 1 1 1 ti 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 O 1 1 1 1 11 2 3540 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 0.0309 0.0067 ORGAN-TOBODY WT (%) 10 0.0043 0.0007 ORGAN-TOBRAIN WT RATIO 10 0.0128 0.0026 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 0.0525 0.0225 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 0.0334 0.0053 10 0.0046 0.0007 10 0.0140 0.0021 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.0435 0.0075 Covance 6329-228 3M T-6316.1 PAGE: 1 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0133 0.0068 10 0.0244 0.0115 10 0.0111 0.0022 10 0.0212 0.0030 3541 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 0.0320 0.0046 ORGAN-TOBODY WT (%) 10 0.0045 0.0006 ORGAN-TOBRAIN WT RATIO 10 0.0132 0.0016 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 0.0384 0.0086 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 0.0286 0.0034 10 0.0040 0.0007 10 0.0120 0.0014 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.0379 0.0072 Covance 6329-228 3M T-6316.1 PAGE: 2 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0096 0.0031 10 0.0177 0.0042 10 0.0096 0.0019 10 0.0186 0.0036 3542 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 2.4158 0.1977 ORGAN-TOBODY WT (%) 10 0.3385 0.0420 ORGAN-TOBRAIN WT RATIO 10 1.0000 0.0000 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 2.1708 0.1447 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 2.3887 0.0908 10 0.3324 0.0345 10 1.0000 0.0000 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 2.0441 0.0983 Covance 6329-228 3M T-6316.1 PAGE: 3 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.5433 0.0978 10 1.0000 0.0000 10 0.5221 0.0618 10 1.0000 0.0000 3543 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT KIDNEY SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 2.3570 0.2803 ORGAN-TOBODY WT (%) 10 0.3279 0.0270 ORGAN-TOBRAIN WT RATIO 10 0.9754 0.0820 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 1.4112 0.2048 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 2.2022 0.3009 10 0.3056 0.0435 10 0.9215 0.1177 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 1.3218 0.1769 Covance 6329-228 3M T-6316.1 PAGE: 4 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.3461 0.0284 10 0.6518 0.0994 10 0.3353 0.0394 10 0.6460 0.0730 3544 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 2.3234 0.2897 ORGAN-TOBODY WT (%) 10 0.3234 0.0315 ORGAN-TOBRAIN WT RATIO 10 0.9622 0.0962 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 1.4370 0.2720 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 2.2647 0.3230 10 0.3144 0.0491 10 0.9474 0.1257 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 1.3601 0.2061 Covance 6329-228 3M T-6316.1 PAGE: 5 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.3507 0.0371 10 0.6636 0.1305 10 0.3449 0.0481 10 0.6642 0.0832 3545 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 20.2343 4.2122 ORGAN-TOBODY WT (%) 10 2.8067 0.4847 ORGAN-TOBRAIN WT RATIO 10 8.3464 1.4431 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 11.3196 2.4524 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 20.3650 2.9205 10 2.8120 0.3074 10 8.5202 1.1115 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 10.9316 1.4863 Covance 6329-228 3M T-6316.1 PAGE: 6 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 2.7467 0.1596 10 5.2305 1.1889 10 2.7642 0.2431 10 5.3527 0.7110 3546 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 2.1758 0.2190 ORGAN-TOBODY WT (%) 10 0.3044 0.0381 ORGAN-TOBRAIN WT RATIO 10 0.9008 0.0625 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 1.6720 0.2500 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 2.0380 0.1979 10 0.2845 0.0431 10 0.8542 0.0877 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 1.4877 0.1285 Covance 6329-228 3M T-6316.1 PAGE: 7 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.4216 0.1081 10 0.7736 0.1347 10 0.3787 0.0404 10 0.7284 0.0599 3547 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT OVARY SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) 10 721.2 89.7 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 0.0564 0.0161 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.0611 0.0183 Covance 6329-228 3M T-6316.1 PAGE: 8 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0143 0.0053 10 0. 0263 0. 0083 10 0.0155 0.0047 10 0. 0297 0. 0081 3548 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT OVARY SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV: TERMINAL BODY WT (g) 10 721.2 89.7 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 0.0544 0.0176 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.0624 0.0105 Covance 6329-228 3M T-6316.1 PAGE: 9 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0140 0.0059 10 0. 0253 0. 0090 10 0.0160 0.0035 10 0. 0305 0. 0044 3549 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 1.0371 0.2467 ORGAN-TOBODY WT (%) 10 0.1430 0.0218 ORGAN-TOBRAIN WT RATIO 10 0.4265 0.0763 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 0.5884 0.0964 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 1.1631 0.2368 10 0.1618 0.0364 10 0.4862 0.0943 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.5933 0.1075 Covance 6329-228 3M T-6316.1 PAGE: 10 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.1446 0.0191 10 0.2721 0.0491 10 0.1516 0.0313 10 0.2911 0.0554 3550 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT TESTIS SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 1.8105 0.1543 ORGAN-TOBODY WT (%) 10 0.2529 0.0240 ORGAN-TOBRAIN WT RATIO 10 0.7539 0.0873 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 10 412.5 85.7 ORGAN WEIGHT (g) M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 1.7120 0.3999 10 0.2403 0.0685 10 0.7138 0.1556 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 Covance 6329-228 3M T-6316.1 PAGE: 11 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 3551 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP M8 NUMBER IN GROUP MEAN: STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 1.8082 0.1769 ORGAN-TOBODY WT (%) 10 0.2523 0.0226 ORGAN-TOBRAIN WT RATIO 10 0.7536 0.1006 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 10 412.5 85.7 ORGAN WEIGHT (g) M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 1.8413 0.2307 10 0.2571 0.0479 10 0.7703 0.0872 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 Covance 6329-228 3M T-6316.1 PAGE: 12 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 3552 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LF THYROID/PARA SEX DOSE GROUP M8 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 0.0232 0.0067 ORGAN-TOBODY WT (%) 10 0.0032 0.0007 ORGAN-TOBRAIN WT RATIO 10 0.0096 0.0026 SEX DOSE GROUP F8 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 0.0142 0.0032 M9 NUMBER IN GROUP MEAN STANDARD DEV 10 725.1 76.6 10 0.0202 0.0050 10 0.0028 0.0008 10 0.0085 0.0021 F9 NUMBER IN GROUP MEAN STANDARD DEV 10 396.4 49.5 10 0.0177 0.0066 Covance 6329-228 3M T-6316.1 PAGE: 13 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0035 0.0007 10 0.0066 0.0017 10 0.0045 0.0016 10 0.0087 0.0034 3553 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT THYROID/PARA SEX DOSE GROUP M8 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 0.0251 0.0079 ORGAN-TOBODY WT (%) 10 0.0035 0.0009 ORGAN-TOBRAIN WT RATIO 10 0.0104 0.0030 SEX DOSE GROUP F8 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 0.0142 0.0025 M9 NUMBER IN GROUP MEAN STANDARD DEV 10 725.1 76.6 10 0.0242 0.0060 10 0.0034 0.0009 10 0.0101 0.0023 F9 NUMBER IN GROUP MEAN STANDARD DEV 10 396.4 49.5 10 0.0147 0.0044 Covance 6329-228 3M T-6316.1 PAGE: 14 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0035 0.0005 10 0.0066 0.0013 10 0.0037 0.0010 10 0.0072 0.0020 3554 Covance 6329-212 Table 26 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS SEX DOSE GROUP M8 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 10 721.2 89.7 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 1.0319 0.1956 M9 NUMBER IN GROUP MEAN STANDARD DEV 10 725.1 76.6 F9 NUMBER IN GROUP MEAN STANDARD DEV 10 396.4 49.5 10 1.0749 0.2526 Covance 6329-228 3M T-6316.1 PAGE: 15 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.2563 0.0631 10 0.4776 0.1015 10 0.2753 0.0763 10 0.5268 0.1225 3555 Covance 6329-212 Table 27 Incidence of Macroscopic Observations Week 4 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=1;SUBSET=T ORGAN AND KEYWORD(S) OR PHRASE ** TOP OF LIST ** LIVER (LI) .................. ** END OF LIST ** -- N U M B E R - O F - A N I M A L S SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 Covance 6329-228 3M T-6316.1 PAGE: 1 A F F E C T E D -- 3556 Covance 6329-212 Table 28 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=2;SUBSET=T ORGAN AND KEYWORD(S) OR PHRASE ** TOP OF LIST ** LIVER (LI) .................. ** END OF LIST ** -- N U M B E R - O F - A N I M A L S SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 Covance 6329-228 3M T-6316.1 PAGE: 1 A F F E C T E D -- 3557 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ** TOP OF LIST ** GENERAL COMMENT (GC) ................ ................. NUMBER EXAMINED: 10 10 10 10 NO MACROSCOPIC LESIONS MALOCCLUSION ANIMAL OBESE BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN 1422 0011 7301 10 10 10 10 0010 BONE, FEMUR (FE) .................... ................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 MARROW, FEMUR (FM) .................. ................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 MARROW, STERNUM (SE) ................ ................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 BONE, STERNUM (SB) .................. ................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 EYE (EY) ............................. ................. NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 1 A F F E C T E D -- 3558 Covance 6329-212 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 10 10 10 10 BRAIN (BR) ................................ ............ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 9 COMPRESSED (HYPOTHALAMUS) 0001 SPINAL CORD (SC) ......................... ............ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LUNG (LU) ................................. ............ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 9 10 LIGHT FOCUS(I)/AREA(S) 0010 KIDNEY (KD) ............................... ............ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 9 10 9 LARGE PELVIS(ES) CYST(S) 0100 0001 LIVER (LI) ................................ ............ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 8 10 9 10 LIGHT FOCUS(I)/AREA(S) MOTTLED RED FOCUS(I)/AREA(S) 1000 1000 0010 3559 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 10 10 10 -=- -=- -=- -=- HEART (HT) ................... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 MUSCLE, SKELETAL (SM) ....... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 NERVE, SCIATIC (SN) ......... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, MESENTERIC (MS) ......... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 SPLEEN (SP) .................. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 9 10 10 LIGHT FOCUS(I)/AREA(S) 0100 THYMUS (TH) .................. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ESOPHAGUS (ES) ............... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 3 A F F E C T E D -- 3560 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 10 10 10 -=- -=- -=- -=- THYROID (TY) ................. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 PARATHYROID (PT) ............. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 TRACHEA (TR) ................. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ADRENAL, CORTEX (AC) ........ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 9 10 LARGE 0010 ADRENAL, MEDULLA (MA) ....... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 PITUITARY (PI) ............... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 95 8 DARK FOCUS(I)/AREA(S) LARGE DIFFUSELY RED ** CONTINUED ON NEXT PAGE ** 0010 0142 0001 3561 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 4 A F F E C T E D -- Covance 6329-212 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 10 10 10 10 ---- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** PITUITARY (PI) ............... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 95 8 RED FOCUS(I)/AREA(S) 0 02 1 HARDERIAN GLAND (HG) ........ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 SALIV GL, MANDIB (SG) ....... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 PANCREAS (PA) ................ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 STOMACH, GL (ST) ............. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 8 10 DARK FOCUS(I)/AREA(S) 002 0 STOMACH, NONGL (SU) ......... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 Covance 6329-228 3M T-6316.1 PAGE: 5 A F F E C T E D -- 3562 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE -------------------------------- -- NUMBER: 10 10 10 10 -=- -=- -=- -=- DUODENUM (DU) ................ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 JEJUNUM (JE) ................. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ILEUM (IL) ................... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 CECUM (CE) ................... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 COLON (CO) ................... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 RECTUM (RE) .................. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 6 A F F E C T E D -- 3563 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 10 10 10 -=- -=- -=- -=- SKIN (SK) .................... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 9 CRUSTED AREA(S) 0001 MAMMARY, FEMALE (MF) ........ ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 10 10 065 MASS(ES) CYST(S) 0012 0034 URINARY BLADDER (UB) ........ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 CERVIX (CV) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 10 10 0 10 10 UTERUS (UT) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: CYST(S) OVARY (OV) ................... ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 10 10 0 10 9 001 0 10 10 0 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 7 A F F E C T E D -- 3564 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 10 10 10 -=- -=- -=- -=- VAGINA (VA) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 10 10 0 10 10 PROSTATE (PR) ................ ........................ NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 10 0 0 SEMINAL VESICLES (SV) ....... ........................ NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 10 0 0 EPIDIDYMIDES (EP) ............ ........................ NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 10 0 0 TESTIS (TE) .................. ........................ NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 9 0 0 SMALL 0100 CAVITY, ORAL (OC) ............ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 URETER (UE) .................. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 8 A F F E C T E D -- 3565 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 10 10 10 -=- -=- -=- -=- CALVARIUM/SKULL (CA) ........ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, AXILLARY (AX) ............ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 9 10 DIFFUSELY RED 0010 LN, MEDIASTINAL (ML) ........ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, MANDIBULAR (MN) ......... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 8 7 DIFFUSELY RED MOTTLED 0022 0001 ADRENAL (AD) ................. ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 FOOT/FOOTPAD (FP) ............ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 9 A F F E C T E D -- 3566 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 10 10 10 -=- -=- -=- -=- TAIL (TI) .................... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 MAMMARY, MALE (MM) .......... ........................ NUMBER EXAMINED: 10 10 0 0 NOT REMARKABLE: 10 10 0 0 LN, LUMBAR/ILIAC (LM) ....... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 AORTA (AO) ................... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, RENAL (RL) ............... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, INGUINAL (IN) ............ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 CAVITY, ABDOM (PC) .......... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 10 A F F E C T E D -- 3567 Table 29 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 10 10 10 10 -=- -=- -=- -=- AUDITORY SEB GL (AS) ........ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 BILE DUCT (BD) ............... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ADIPOSE TISSUE (AT) ......... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, OTHER (LN) ............... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, ANT MES/PANC (AP) ....... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 JOINT, OTHER (JT) ............ ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 LN, POPLITEAL (PP) .......... ........................ NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 10 ** END OF LIST ** 3568 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 11 A F F E C T E D -- Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ** TOP OF LIST ** GENERAL COMMENT (GC) ........ ........................ NUMBER EXAMINED: 11 20 13 15 NO MACROSCOPIC LESIONS BLOOD FILM TAKEN MALOCCLUSION STAINS-PERINEUM/PERIANAL ANIMAL OBESE DISCHARGE-OCULAR ANIMAL THIN BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN STAINS - SKIN/HAIRCOAT 0100 11 20 13 14 1111 3100 1000 2 001 3110 11 20 13 14 1011 0 02 1 BONE, FEMUR (FE) ............. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 MARROW, FEMUR (FM) ................. .................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 MARROW, STERNUM (SE) ................ ................. NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 3569 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 11 20 13 15 -=- -=- -=- -=- BONE, STERNUM (SB) .......... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 EYE (EY) ..................... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 15 WHITE INTRAOCULAR MATERIAL 0100 BRAIN (BR) ................... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 7 18 8 5 COMPRESSED (HYPOTHALAMUS) 4 2 5 10 SPINAL CORD (SC) ............. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 LUNG (LU) .................... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 14 LIGHT FOCUS(I)/AREA(S) MOTTLED 0001 0100 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 2 A F F E C T E D -- 3570 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 11 20 13 15 KIDNEY (KD) ............................... ............ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 8 16 13 15 LARGE PELVIS(ES) ROUGH SURFACE, DIFFUSE MASS(ES) GRANULAR MATERIAL DIFFUSELY LIGHT LARGE 12 00 12 00 1000 1100 1300 02 00 LIVER (LI) ................................ ............ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 6 9 6 6 LIGHT FOCUS(I)/AREA(S) MOTTLED RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) LARGE RAISED FOCUS(I)/AREA(S) CYST(S) DARK FOCUS(I)/AREA(S) 022 0 0110 4734 0100 1100 0110 0001 0001 0103 HEART (HT) ................................ ............ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 LIGHT FOCUS(I)/AREA(S) 0010 3571 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 11 20 13 15 MUSCLE, SKELETAL (SM) ....... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 14 MASS(ES) 0001 NERVE, SCIATIC (SN) ......... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 LN, MESENTERIC (MS) ......... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 18 13 15 DIFFUSELY RED LARGE 02 00 0100 SPLEEN (SP) .................. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 17 12 14 LARGE RAISED FOCUS(I)/AREA(S) 0111 02 00 THYMUS (TH) .................. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 MASS(ES) 0010 3572 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 11 20 13 15 -=- -=- -=- -=- ESOPHAGUS (ES) ............... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 THYROID (TY) ................. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 19 13 15 LARGE 1100 PARATHYROID (PT) ............. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 20 13 14 LARGE 1001 TRACHEA (TR) ................. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 ADRENAL, CORTEX (AC) ........ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 8 12 LARGE DARK FOCUS(I)/AREA(S) MASS(ES) 0 02 3 0010 002 0 ADRENAL, MEDULLA (MA) ....... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 5 A F F E C T E D -- 3573 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 11 20 13 15 -=- -=- -=- -=- PITUITARY (PI) ............... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 4 11 2 3 MASS(ES) LARGE DIFFUSELY RED RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) 5 2 7 10 1 02 1 0110 1511 1010 0100 HARDERIAN GLAND (HG) ........ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 SALIV GL, MANDIB (SG) ....... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 PANCREAS (PA) ................ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 18 12 15 LARGE VESSELS MASS(ES) 1000 02 1 0 3574 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 11 20 13 15 STOMACH, GL (ST) ......................... ............ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 7 14 12 14 DARK FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL 1310 2000 14 01 STOMACH, NONGL (SU) ...................... ............ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 15 EROSION/ULCERATION 0100 DUODENUM (DU) ............................. ............ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 JEJUNUM (JE) .............................. ............ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 DIVERTICULUM(A) 0010 ILEUM (IL) ................................ ............ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 CECUM (CE) ................................ ............ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 Covance 6329-228 3M T-6316.1 PAGE: 7 A F F E C T E D -- 3575 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 11 20 13 15 -=- -=- -=- -=- COLON (CO) ................... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 RECTUM (RE) .................. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 SKIN (SK) .................... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 7 16 11 12 CRUSTED AREA(S) MASS(ES) ABRASION(S)/ULCERATION(S) RAISED AREA(S) 2212 1101 0110 1100 MAMMARY, FEMALE (MF) ........ ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 13 15 068 MASS(ES) THICKENED MASS #2 MASS #3 0077 0 02 1 0044 0010 URINARY BLADDER (UB) ........ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 3576 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 11 20 13 15 CERVIX (CV) ...................... .................... NUMBER EXAMINED: NOT REMARKABLE: LARGE UTERUS (UT) ...................... .................... NUMBER EXAMINED: NOT REMARKABLE: CYST(S) MASS(ES) OVARY (OV) ....................... .................... NUMBER EXAMINED: NOT REMARKABLE: CYST(S) MASS(ES) VAGINA (VA) ...................... .................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 13 15 0 13 14 001 0 13 15 0 11 13 012 010 0 13 15 0 8 10 055 010 0 13 15 0 13 15 PROSTATE (PR) .................... .................... NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 11 20 0 0 SEMINAL VESICLES (SV) ............ .................... NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 11 20 0 0 3577 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 11 20 13 15 -=- -=- -=- -=- EPIDIDYMIDES (EP) ............ ........................ NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 11 20 0 0 ANIMALS TESTIS (TE) .................. ........................ NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 8 16 0 0 SMALL CONTAINS FLUID RED/YELLOW FOCUS(I)/AREA(S) 3300 1000 02 00 CAVITY, ORAL (OC) ............ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 URETER (UE) .................. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 CALVARIUM/SKULL (CA) ........ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 LN, AXILLARY (AX) ............ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 12 14 LARGE ** CONTINUED ON NEXT PAGE ** 0110 Covance 6329-228 3M T-6316.1 PAGE: 10 A F F E C T E D -- 3578 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 11 20 13 15 ** FROM PREVIOUS PAGE ** LN, AXILLARY (AX) ............ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 12 14 MOTTLED 0111 LN, MEDIASTINAL (ML) ........ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 MOTTLED 0010 LN, MANDIBULAR (MN) ......... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 11 14 DIFFUSELY RED MOTTLED LARGE 0010 0010 0011 ADRENAL (AD) ................. ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 T E D -- FOOT/FOOTPAD (FP) ............ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 8 18 11 12 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) 302 0 02 03 3579 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 11 20 13 15 T E D -- TAIL (TI) .................... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 18 13 14 RAISED AREA(S) ULCERATION(S)/ABRASION(S) 02 00 0001 MAMMARY, MALE (MM) .......... ........................ NUMBER EXAMINED: 11 20 0 0 NOT REMARKABLE: 10 20 0 0 MASS(ES) 1000 LN, LUMBAR/ILIAC (LM) ....... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 19 13 14 LARGE 1101 AORTA (AO) ................... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 15 LARGE 0100 LN, RENAL (RL) ............... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 10 19 13 15 LARGE DIFFUSELY RED 1100 1100 3580 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 11 20 13 15 -=- -=- -=- -=- LN, INGUINAL (IN) ............ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 LARGE 0010 CAVITY, ABDOM (PC) .......... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 AUDITORY SEB GL (AS) ........ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 BILE DUCT (BD) ............... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 19 13 15 LARGE 0100 ADIPOSE TISSUE (AT) ......... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 LN, OTHER (LN) ............... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 13 15 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 13 A F F E C T E D -- 3581 Covance 6329-212 Table 30 Incidence of Macroscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 11 20 13 15 T E D -- LN, ANT MES/PANC (AP) ....... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 18 13 15 LARGE DIFFUSELY RED 0100 0100 JOINT, OTHER (JT) ............ ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 9 20 13 14 LARGE 2 001 LN, POPLITEAL (PP) .......... ........................ NUMBER EXAMINED: 11 20 13 15 NOT REMARKABLE: 11 20 12 15 LARGE MOTTLED ** END OF LIST ** 0010 0010 3582 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 -- ------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ......... ....................... NUMBER EXAMINED: 39 30 37 35 BLOOD FILM TAKEN MALOCCLUSION STAINS-PERINEUM/PERIANAL URINARY BLADDER-LARGE LUMEN AUTOLYSIS-SEVERE ANIMAL OBESE GI TRACT-LIGHT FLUID CONTENT GI TRACT-LARGE LUMEN DISCHARGE-OCULAR DISCHARGE-NASAL ANIMAL THIN TEETH MISSING/BROKEN/WORN GI TRACT-DARK FLUID CONTENT STAINS - SKIN/HAIRCOAT DISCHARGE-ORAL FLUID-THORACIC CAVITY 29 19 28 30 4475 12 5 8 7 32 00 2000 4312 0010 0110 12 8 19 14 7 5 10 5 7 8 12 11 02 2 1 112 0 602 0 0110 1000 BONE, FEMUR (FE) .............. ....................... NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 MARROW, FEMUR (FM) ................. .................. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3583 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 39 30 37 35 -=- -=- -=- -=- MARROW, STERNUM (SE) ........ ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 ANIMALS BONE, STERNUM (SB) .......... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 36 35 MISALIGNED 0010 EYE (EY) ..................... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 OPAQUE CORNEA 0100 BRAIN (BR) ................... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 18 14 5 6 COMPRESSED (HYPOTHALAMUS) 21 16 32 29 SPINAL CORD (SC) ............. ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 LUNG (LU) .................... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 26 36 32 LIGHT FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 0110 Covance 6329-228 3M T-6316.1 PAGE: 2 A F F E C T E D -- 3584 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 -- -- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LUNG (LU) ................................. ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 26 36 32 UNCOLLAPSED RED FOCUS(I)/AREA(S) ADHESION(S) DIFFUSELY RED 02 03 0001 02 00 1100 KIDNEY (KD) ............................... ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 25 19 33 33 LARGE PELVIS(ES) LIGHT FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE MASS(ES) CYST(S) GRANULAR MATERIAL DIFFUSELY LIGHT DIFFUSELY DARK LARGE MOTTLED 8511 0010 4800 1000 1100 1112 7501 0010 2200 0010 LIVER (LI) ................................ ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 21 19 27 28 LIGHT FOCUS(I)/AREA(S) MOTTLED ** CONTINUED ON NEXT PAGE ** 1512 3 02 1 3585 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 --------------------------------------------- -- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ................................ ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 21 19 27 28 RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) LARGE DEPRESSED FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) CYST(S) DARK FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE 4241 4321 2200 32 01 1010 1001 1001 0100 1000 HEART (HT) ................................ ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 27 37 34 LIGHT FOCUS(I)/AREA(S) MASS(ES) 1301 1000 MUSCLE, SKELETAL (SM) .................... ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 MASS(ES) 0100 NERVE, SCIATIC (SN) ...................... ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3586 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 39 30 37 35 LN, MESENTERIC (MS) ......... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 36 35 DIFFUSELY RED LARGE 0010 0100 SPLEEN (SP) .................. ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 26 37 32 LIGHT FOCUS(I)/AREA(S) DIFFUSELY LIGHT LARGE ROUGH SURFACE DEPRESSED FOCUS(I)/AREA(S) MASS(ES) 0100 0001 2 1 02 1000 0100 0100 THYMUS (TH) .................. ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 34 LARGE 0101 ESOPHAGUS (ES) ............... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3587 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 39 30 37 35 THYROID (TY) ................. ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 27 37 34 LARGE MASS(ES) DIFFUSELY RED 12 00 0100 0001 PARATHYROID (PT) ............. ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 26 37 35 LARGE 2400 TRACHEA (TR) ................. ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 ADRENAL, CORTEX (AC) ................... .............. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 25 31 27 LARGE LIGHT FOCUS (I)/AREA(S) SMALL MISSING/NOT IDENTIFIED DIFFUSELY RED MOTTLED DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 04 4 8 0010 0010 1000 1000 0012 0100 1000 3588 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ....... DIFFUSELY LIGHT ADRENAL, MEDULLA (MA) -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 39 30 37 35 NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 25 31 27 0100 NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 PITUITARY (PI) MASS(ES) DARK FOCUS(I)/AREA(S) LARGE DIFFUSELY RED RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) HARDERIAN GLAND (HG) .. NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 11 13 2 4 20 16 31 29 002 0 2 012 1000 1110 3000 1010 NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 SALIV GL, MANDIB (SG) NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3589 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 39 30 37 35 PANCREAS (PA) ............................. ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 26 36 35 LARGE VESSELS MASS(ES) 02 00 02 1 0 STOMACH, GL (ST) ......................... ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 23 19 35 33 RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL ADHESION(S) 4 01 0 3812 1100 2100 82 00 0100 STOMACH, NONGL (SU) ...................... ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 31 27 32 32 THICKENED WALL EROSION/ULCERATION DARK FOCUS(I)/AREA(S) 6231 5142 0100 DUODENUM (DU) ............................. ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 30 37 35 RED FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 1000 3590 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 -- ------------------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** DUODENUM (DU) ................ ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 30 37 35 THICKENED WALL 1000 JEJUNUM (JE) ................. ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 30 37 35 RED FOCUS(I)/AREA(S) 1000 ILEUM (IL) ................... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 36 35 LARGE LYMPHOID AREA(S) 0010 CECUM (CE) ................... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 29 37 32 THICKENED WALL MASS(ES) RED FOCUS(I)/AREA(S) 1 1 02 0002 1000 COLON (CO) ................... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 3591 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 39 30 37 35 T E D -- RECTUM (RE) ............................................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 SKIN (SK) ............................. ................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 35 24 36 34 CRUSTED AREA(S) MASS(ES) SUBCUTANEOUS EDEMA ALOPECIA-DIFFUSE 3510 0101 1000 0100 MAMMARY, FEMALE (MF) ................. ................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 37 35 0 11 13 MASS(ES) CYST(S) THICKENED MASS #2 MASS #3 0 0 19 18 0077 0 0 10 9 0 0 11 9 0 04 3 URINARY BLADDER (UB) ................. ................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 28 36 35 CALCULUS(I) THICKENED WALL RED FOCUS(I)/AREA(S) ** CONTINUED ON NEXT PAGE ** 2 01 0 2000 1000 3592 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) ........ ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 28 36 35 DIFFUSELY RED SEMIFLUID MATERIAL 12 00 1000 CERVIX (CV) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 37 35 0 36 34 LARGE 0011 UTERUS (UT) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 37 35 0 32 28 CYST(S) MASS(ES) SMALL THICKENED WALL 0 04 6 0002 0010 0001 OVARY (OV) ................... ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 37 35 0 31 28 DIFFUSELY RED CYST(S) 0010 0057 3593 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 39 30 37 35 T E D -- VAGINA (VA) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 37 35 0 33 27 SEMIFLUID MATERIAL THICKENED WALL 0 04 8 0001 PROSTATE (PR) ................ ........................ NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 30 20 0 0 GELATINOUS NODULAR MASS(ES) LARGE SMALL DIFFUSELY RED 1000 7600 0100 1100 02 00 1000 SEMINAL VESICLES (SV) ....... ........................ NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 31 25 0 0 GELATINOUS MASS(ES) LARGE SMALL 12 00 1000 12 00 5100 3594 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 39 30 37 35 T E D -- EPIDIDYMIDES (EP) ........................ ............ NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 39 28 0 0 MASS(ES) SMALL 0100 0100 TESTIS (TE) ............................... ............ NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 34 29 0 0 SMALL 5100 CAVITY, ORAL (OC) ........................ ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 36 35 ABRASION(S)/ULCERATION(S) 0010 URETER (UE) ............................... ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 30 37 35 LARGE 2000 CALVARIUM/SKULL (CA) ..................... ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 29 36 35 FRACTURE RED FOCUS(I)/AREA(S) MASS(ES) 0010 1000 0100 3595 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 39 30 37 35 -=- -=- -=- -=- LN, AXILLARY (AX) ............ ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 32 31 LARGE DIFFUSELY RED MOTTLED 0042 0022 0001 LN, MEDIASTINAL (ML) ........ ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 30 37 34 DIFFUSELY RED LARGE 1001 1000 LN, MANDIBULAR (MN) ......... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 29 33 32 DIFFUSELY RED MOTTLED LARGE 2 011 1011 0121 ADRENAL (AD) ................. ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 T E D -- 3596 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 39 30 37 35 FOOT/FOOTPAD (FP) ........................ ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 31 26 37 32 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) LARGE 42 02 4200 0001 TAIL (TI) ................................. ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 37 29 37 35 CRUSTED AREA(S) RAISED AREA(S) 1100 1000 MAMMARY, MALE (MM) ....................... ............ NUMBER EXAMINED: 39 30 0 0 NOT REMARKABLE: 32 26 0 0 THICKENED MASS(ES) CYST(S) 0100 52 00 2100 LN, LUMBAR/ILIAC (LM) .................... ............ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 24 37 35 MASS(ES) LARGE DIFFUSELY DARK CYST(S) ** CONTINUED ON NEXT PAGE ** 0100 1300 0100 1100 3597 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 39 30 37 35 ** FROM PREVIOUS PAGE ** LN, LUMBAR/ILIAC (LM) ....... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 36 24 37 35 MOTTLED 1000 AORTA (AO) ................... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 38 29 37 35 LARGE FIRM 1100 1100 LN, RENAL (RL) ............... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 28 37 35 LARGE DIFFUSELY RED 02 00 02 00 LN, INGUINAL (IN) ............ ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 36 35 LARGE 0010 CAVITY, ABDOM (PC) .......... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 MASS(ES) 0100 T E D -- 3598 Covance 6329-212 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 39 30 37 35 T E D -- AUDITORY SEB GL (AS) ........ ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 MASS(ES) 0100 BILE DUCT (BD) ............... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 LARGE 0100 ADIPOSE TISSUE (AT) ......... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 34 MASS(ES) THICKENED 0100 0001 LN, OTHER (LN) ............... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 LARGE 0100 LN, ANT MES/PANC (AP) ....... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 29 37 35 LARGE 0100 3599 Table 31 Incidence of Macroscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;SUBSET=ALL -- N U M B E R - < SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE -------------------------------- -- NUMBER: 39 30 37 35 -=- -=- -=- -=- JOINT, OTHER (JT) ............ ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 LN, POPLITEAL (PP) .......... ........................ NUMBER EXAMINED: 39 30 37 35 NOT REMARKABLE: 39 30 37 35 ** END OF LIST ** Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 18 T E D -- 3600 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 -- ------------------------------------------ -=- -=- -=- -=- ** TOP OF LIST ** GENERAL COMMENT (GC) ......... ....................... NUMBER EXAMINED: 70 70 70 70 NO MACROSCOPIC LESIONS BLOOD FILM TAKEN MALOCCLUSION STAINS-PERINEUM/PERIANAL URINARY BLADDER-LARGE LUMEN AUTOLYSIS-SEVERE ANIMAL OBESE GI TRACT-LIGHT FLUID CONTENT GI TRACT-LARGE LUMEN DISCHARGE-OCULAR DISCHARGE-NASAL ANIMAL THIN BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN GI TRACT-DARK FLUID CONTENT STAINS - SKIN/HAIRCOAT DISCHARGE-ORAL FLUID-THORACIC CAVITY 11 15 12 12 40 39 41 44 5597 15 6 8 7 32 00 2000 12 6 1 3 0010 0110 14 8 19 15 7 5 10 5 10 9 13 11 21 30 23 24 1242 112 0 6 04 1 0110 1000 BONE, FEMUR (FE) .............. ....................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 3601 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE ---- NUMBER: 70 70 70 70 -=- -=- -=- -=- MARROW, FEMUR (FM) .......... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 MARROW, STERNUM (SE) ........ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 BONE, STERNUM (SB) .......... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 69 70 MISALIGNED 0010 EYE (EY) ..................... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 68 70 70 OPAQUE CORNEA WHITE INTRAOCULAR MATERIAL 0100 0100 BRAIN (BR) ................... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 45 52 33 30 COMPRESSED (HYPOTHALAMUS) 25 18 37 40 SPINAL CORD (SC) ............. ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 2 A F F E C T E D -- 3602 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 70 70 70 70 LUNG (LU) ................................. ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 65 68 66 LIGHT FOCUS(I)/AREA(S) UNCOLLAPSED RED FOCUS(I)/AREA(S) ADHESION(S) DIFFUSELY RED MOTTLED 0121 02 03 0001 02 00 1100 0100 KIDNEY (KD) ............................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 53 54 66 67 LARGE PELVIS(ES) LIGHT FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE MASS(ES) CYST(S) GRANULAR MATERIAL DIFFUSELY LIGHT DIFFUSELY DARK LARGE MOTTLED 9811 0010 5 10 0 0 2000 1101 2212 8801 0010 2400 0010 3603 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 70 70 70 70 LIVER (LI) ................................ ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 45 48 52 54 LIGHT FOCUS(I)/AREA(S) MOTTLED RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) LARGE DEPRESSED FOCUS(I)/AREA(S) RAISED FOCUS(I)/AREA(S) CYST(S) DARK FOCUS(I)/AREA(S) ROUGH SURFACE, DIFFUSE 2732 4131 8985 4421 3300 3311 1010 1 0 02 1 0 02 02 03 1000 HEART (HT) ................................ ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 67 69 69 LIGHT FOCUS(I)/AREA(S) MASS(ES) 1311 1000 MUSCLE, SKELETAL (SM) .................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 69 MASS(ES) 0101 3604 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 70 70 70 70 NERVE, SCIATIC (SN) ...................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 LN, MESENTERIC (MS) ...................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 67 69 70 DIFFUSELY RED LARGE 02 1 0 02 00 SPLEEN (SP) ............................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 62 69 66 LIGHT FOCUS(I)/AREA(S) DIFFUSELY LIGHT LARGE ROUGH SURFACE DEPRESSED FOCUS(I)/AREA(S) MASS(ES) RAISED FOCUS(I)/AREA(S) 02 00 0001 2213 1000 0100 0100 02 00 THYMUS (TH) ............................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 69 69 LARGE MASS(ES) 0101 0010 3605 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 70 70 70 70 ESOPHAGUS (ES) .................... ................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 THYROID (TY) ...................... ................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 66 70 69 LARGE MASS(ES) DIFFUSELY RED 2300 0100 0001 PARATHYROID (PT) .................. ................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 66 70 69 LARGE 34 01 TRACHEA (TR) ...................... ................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 ADRENAL, CORTEX (AC) .............. ................... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 65 58 59 LARGE LIGHT FOCUS (I)/AREA(S) SMALL MISSING/NOT IDENTIFIED ** CONTINUED ON NEXT PAGE ** 0 4 7 11 0010 0010 1000 3606 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 -- ------------------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ........ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 65 58 59 DIFFUSELY RED MOTTLED DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) DIFFUSELY LIGHT MASS(ES) 1000 0012 0110 1000 0100 002 0 ADRENAL, MEDULLA (MA) ....... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 PITUITARY (PI) ........................ ............... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 35 43 19 25 MASS(ES) DARK FOCUS(I)/AREA(S) LARGE DIFFUSELY RED RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) 25 18 38 39 0030 3175 1111 2642 4 01 0 1110 HARDERIAN GLAND (HG) .................. ............... NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 3607 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 70 70 70 70 SALIV GL, MANDIB (SG) .................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 PANCREAS (PA) ............................. ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 64 68 70 LARGE VESSELS MASS(ES) 12 00 042 0 STOMACH, GL (ST) ......................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 50 53 65 67 RED FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) LIGHT FOCUS(I)/AREA(S) EROSION/ULCERATION THICKENED WALL ADHESION(S) 4 01 0 4 11 4 2 1100 4100 9601 0100 STOMACH, NONGL (SU) ...................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 62 66 65 67 THICKENED WALL EROSION/ULCERATION DARK FOCUS(I)/AREA(S) 6231 5242 0100 3608 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 70 70 70 70 DUODENUM (DU) ............................. ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 70 70 70 RED FOCUS(I)/AREA(S) THICKENED WALL 1000 1000 JEJUNUM (JE) .............................. ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 70 69 70 RED FOCUS(I)/AREA(S) DIVERTICULUM(A) 1000 0010 ILEUM (IL) ................................ ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 69 70 LARGE LYMPHOID AREA(S) 0010 CECUM (CE) ................................ ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 69 70 67 THICKENED WALL MASS(ES) RED FOCUS(I)/AREA(S) 1 1 02 0002 1000 COLON (CO) ................................ ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 3609 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 -- ------------------------------------------- -=- -=- -=- -=- RECTUM (RE) .................. ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 T E D -- SKIN (SK) .................... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 62 60 67 65 CRUSTED AREA(S) MASS(ES) SUBCUTANEOUS EDEMA ALOPECIA-DIFFUSE ABRASION(S)/ULCERATION(S) RAISED AREA(S) 5723 12 02 1000 0100 0110 1100 MAMMARY, FEMALE (MF) ........ ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 70 70 0 33 36 MASS(ES) CYST(S) THICKENED MASS #2 MASS #3 0 0 27 27 0 0 10 11 0 0 12 10 0 0 15 13 0053 URINARY BLADDER (UB) ........ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 68 69 70 CALCULUS(I) ** CONTINUED ON NEXT PAGE ** 2 01 0 3610 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) ........ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 68 69 70 THICKENED WALL RED FOCUS(I)/AREA(S) DIFFUSELY RED SEMIFLUID MATERIAL 2000 1000 12 00 1000 CERVIX (CV) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 70 70 0 69 68 LARGE 0012 UTERUS (UT) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 70 70 0 63 60 CYST(S) MASS(ES) SMALL THICKENED WALL 0059 0012 0010 0001 OVARY (OV) ................... ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 70 70 0 59 58 DIFFUSELY RED CYST(S) MASS(ES) 0010 0 0 10 12 0010 T E D -- 3611 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 70 70 70 70 T E D -- VAGINA (VA) .................. ........................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 70 70 0 66 62 SEMIFLUID MATERIAL THICKENED WALL 0 04 8 0001 PROSTATE (PR) ................ ........................ NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 61 60 0 0 GELATINOUS NODULAR MASS(ES) LARGE SMALL DIFFUSELY RED 1000 7600 0100 1100 02 00 1000 SEMINAL VESICLES (SV) ....... ........................ NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 62 65 0 0 GELATINOUS MASS(ES) LARGE SMALL 12 00 1000 12 00 5100 3612 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 70 70 70 70 T E D -- EPIDIDYMIDES (EP) ........................ ............ NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 70 68 0 0 MASS(ES) SMALL 0100 0100 TESTIS (TE) ............................... ............ NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 62 64 0 0 SMALL CONTAINS FLUID RED/YELLOW FOCUS(I)/AREA(S) 8500 1000 02 00 CAVITY, ORAL (OC) ........................ ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 69 70 ABRASION(S)/ULCERATION(S) 0010 URETER (UE) ............................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 70 70 70 LARGE 2000 CALVARIUM/SKULL (CA) ..................... ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 69 69 70 FRACTURE ** CONTINUED ON NEXT PAGE ** 0010 3613 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 70 70 70 70 ---- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** CALVARIUM/SKULL (CA) ........ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 69 69 70 RED FOCUS(I)/AREA(S) MASS(ES) 1000 0100 LN, AXILLARY (AX) ............ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 63 65 LARGE DIFFUSELY RED MOTTLED 0152 0032 0112 LN, MEDIASTINAL (ML) ........ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 70 69 69 DIFFUSELY RED LARGE MOTTLED 1001 1000 0010 LN, MANDIBULAR (MN) ......... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 67 69 62 63 DIFFUSELY RED MOTTLED LARGE 2 043 1 02 2 0132 T E D -- 3614 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 70 70 70 70 T E D -- ADRENAL (AD) .............................. ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 70 70 FOOT/FOOTPAD (FP) ........................ ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 59 64 68 64 CRUSTED AREA(S) ABRASION(S)/ULCERATION(S) LARGE 7222 44 03 0001 TAIL (TI) ................................. ............ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 67 70 69 CRUSTED AREA(S) RAISED AREA(S) ULCERATION(S)/ABRASION(S) 1100 12 00 0001 MAMMARY, MALE (MM) ....................... ............ NUMBER EXAMINED: 70 70 0 0 NOT REMARKABLE: 62 66 0 0 THICKENED MASS(ES) CYST(S) 0100 62 00 2100 3615 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 70 70 70 70 LN, LUMBAR/ILIAC (LM) ....... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 66 63 70 69 MASS(ES) LARGE DIFFUSELY DARK CYST(S) MOTTLED 0100 24 01 0100 1100 1000 AORTA (AO) ................... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 68 70 70 LARGE FIRM 12 00 1100 LN, RENAL (RL) ............... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 69 67 70 70 LARGE DIFFUSELY RED 1300 1300 LN, INGUINAL (IN) ............ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 68 70 LARGE 002 0 3616 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 70 70 70 70 T E D -- CAVITY, ABDOM (PC) .......... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 70 MASS(ES) 0100 AUDITORY SEB GL (AS) ........ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 70 MASS(ES) 0100 BILE DUCT (BD) ............... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 68 70 70 LARGE 02 00 ADIPOSE TISSUE (AT) ......... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 69 MASS(ES) THICKENED 0100 0001 LN, OTHER (LN) ............... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 69 70 70 LARGE 0100 3617 Covance 6329-212 Table 32 Incidence of Macroscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 70 70 70 70 T E D -- LN, ANT MES/PANC (AP) ....... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 67 70 70 LARGE DIFFUSELY RED 02 00 0100 JOINT, OTHER (JT) ............ ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 68 70 70 69 LARGE 2 001 LN, POPLITEAL (PP) .......... ........................ NUMBER EXAMINED: 70 70 70 70 NOT REMARKABLE: 70 70 69 70 LARGE MOTTLED ** END OF LIST ** 0010 0010 3618 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ** TOP OF LIST ** LUNG (LU) .......................................... ... NUMBER EXAMINED: NOT REMARKABLE: 10 10 10 9 --INFILTRATE, MACROPHAGE, ALVEOLAR 01 KIDNEY (KD) ........................................ ... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 5 1 --CAST, PROTEINACEOUS --MINERALIZATION, PELVIC --NEPHROPATHY, CHRONIC PROGRESSIVE 32 12 26 LIVER (LI) ......................................... ... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 7 6 --DEGENERATION, CYSTIC --HEMORRHAGE --INFLAMMATION, CHRONIC --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR 01 20 12 10 10 02 01 PANCREAS (PA) ...................................... ... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 10 6 --PIGMENT --B-ADENOMA, ISLET CELL 03 01 3619 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D SEX: --MALE- GROUP: -8- -9NUMBER: 10 10 THYROID (TY) --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL PARATHYROID (PT) ......... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 8 6 NUMBER EXAMINED: 10 NOT REMARKABLE: 10 TESTIS (TE) ............................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 --ATROPHY/DEGENERATION 0 SKIN (SK) .............................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 10 9 1 MAMMARY, FEMALE (MF) .................................. NUMBER EXAMINED: NOT REMARKABLE: BONE, FEMUR (FE) ...................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 3620 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O SEX: --MALE- GROUP: -8- -9 NUMBER: 10 10 F-ANIMALS A F F E C T E D -- MARROW, FEMUR (FM) ......... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 MARROW, STERNUM (SE) ....... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 BONE, STERNUM (SB) ......... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 EYE (EY) .................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 BRAIN (BR) .................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 SPINAL CORD (SC) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 MUSCLE, SKELETAL (SM) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 3621 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: --MALE-GROUP: -8- -9NUMBER: 10 10 O F A N I M A L S A F F E C T E D -- NERVE, SCIATIC (SN) ........ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 HEART (HT) .................. --DEGENERATION/NECROSIS LN, MESENTERIC (MS) ........ NUMBER EXAMINED: 10 NOT REMARKABLE: 9 1 NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 0 0 0 SPLEEN (SP) ................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 1 1 THYMUS (TH) ................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 ESOPHAGUS (ES) .............. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 TRACHEA (TR) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 3622 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: --MALE-GROUP: -8- -9NUMBER: 10 10 O F A N I M A L S A F F E C T E D -- ADRENAL, CORTEX (AC) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 ADRENAL, MEDULLA (MA) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 PITUITARY (PI) --HEMORRHAGE --B-ADENOMA HARDERIAN GLAND (HG) --ONE EXAMINED SALIV GL, MANDIB (SG) NUMBER EXAMINED: NOT REMARKABLE: 9 8 0 1 NUMBER EXAMINED: 10 NOT REMARKABLE: 9 1 NUMBER EXAMINED: 10 NOT REMARKABLE: 10 1 0 1 1 0 0 0 0 0 STOMACH, GL (ST) ... --EDEMA NUMBER EXAMINED: 10 NOT REMARKABLE: 9 1 0 0 0 3623 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: --MALE-GROUP: -8- -9NUMBER: 10 10 O F A N I M A L S A F F E C T E D -- STOMACH, NONGL (SU) ........ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 DUODENUM (DU) ............... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 JEJUNUM (JE) ................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 ILEUM (IL) .................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 CECUM (CE) .................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 COLON (CO) .................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 RECTUM (RE) ................. --PARASITISM NUMBER EXAMINED: 10 NOT REMARKABLE: 9 1 0 0 0 3624 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE- GROUP: -8- -9NUMBER: 10 10 URINARY BLADDER (UB) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 UTERUS (UT) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 OVARY (OV) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 CERVIX (CV) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 VAGINA (VA) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 PROSTATE (PR) ................... --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE NUMBER EXAMINED: 10 NOT REMARKABLE: 8 0 0 10 10 3625 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: --MALE-GROUP: -8- -9NUMBER: 10 10 O F A N I M A L S A F F E C T E D -- SEMINAL VESICLES (SV) ...... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 EPIDIDYMIDES (EP) .......... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 DEATH COMMENT (DC) ......... --SCHEDULED SACRIFICE LN, MANDIBULAR (MN) ........ NUMBER EXAMINED: 10 NOT REMARKABLE: 0 10 NUMBER EXAMINED: NOT REMARKABLE: 0 0 10 0 10 0 0 LN, AXILLARY (AX) .......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 FOOT/FOOTPAD (FP) .......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, INGUINAL (IN) .......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3626 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -GROUP: -8- -9NUMBER: 10 10 AORTA (AO) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, RENAL (RL) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, LUMBAR/ILIAC (LM) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 HEMATO NEOPLASIA (HN) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 MAMMARY, MALE (MM) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 CAVITY, ABDOM (PC) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 TAIL (TI) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3627 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE- GROUP: -8- -9NUMBER: 10 10 T E D -- URETER (UE) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 CAVITY, ORAL (OC) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 CALVARIUM/SKULL (CA) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 LN, MEDIASTINAL (ML) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 LN, OTHER (LN) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 BILE DUCT (BD) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 AUDITORY SEB GL (AS) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 3628 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE- GROUP: -8- -9NUMBER: 10 10 T E D -- LN, ANT MES/PANC (AP) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 ADIPOSE TISSUE (AT) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 JOINT, OTHER (JT) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 LN, POPLITEAL (PP) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 NASAL TURBINATE (NT) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 3629 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 10 10 ** TOP OF LIST ** LUNG (LU) ..................................... ....... NUMBER EXAMINED: NOT REMARKABLE: 10 7 10 9 --HEMORRHAGE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --MINERALIZATION, VASCULAR 10 01 10 10 KIDNEY (KD) ................................... ....... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 5 5 --CAST, PROTEINACEOUS --CYST --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC 23 11 01 01 44 LIVER (LI) .................................... ....... NUMBER EXAMINED: 10 10 NOT REMARKABLE: 9 9 --HEMORRHAGE --HYPERPLASIA, BILE DUCT --NECROSIS, HEPATOCELLULAR 10 01 10 PANCREAS (PA) ................................. ....... NUMBER EXAMINED: NOT REMARKABLE: 9 10 98 --ATROPHY, ACINAR 02 T E D -- 3630 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 T E D -- THYROID (TY) ............................ .............. NUMBER EXAMINED: 10 NOT REMARKABLE: 8 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --INFLAMMATION, CHRONIC 0 2 1 PARATHYROID (PT) ....................... .............. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 10 8 1 1 0 0 0 TESTIS (TE) ............................. .............. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 SKIN (SK) ............................... .............. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 --EROSION --INFLAMMATION, ACUTE 0 0 MAMMARY, FEMALE (MF) ................... .............. NUMBER EXAMINED: 10 NOT REMARKABLE: 6 --FIBROSIS --GALACTOCELE --HEMORRHAGE --MINERALIZATION 0 3 0 1 1 0 1 1 10 4 1 6 1 1 3631 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION ** FROM PREVIOUS PAGE ** MAMMARY, FEMALE (MF) .. --B-FIBROADENOMA BONE, FEMUR (FE) .. -- N U M B E R SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 NUMBER EXAMINED: 10 NOT REMARKABLE: 6 1 NUMBER EXAMINED: 10 NOT REMARKABLE: 10 10 4 1 0 0 O F A N I M A L S A F F E C T E D -- MARROW, FEMUR (FM) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 MARROW, STERNUM (SE) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 BONE, STERNUM (SB) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 EYE (EY) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 3632 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 O F A N I M A L S A F F E C T E D -- BRAIN (BR) .................. --COMPRESSION, VENTRAL SPINAL CORD (SC) ............ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 NUMBER EXAMINED: 10 NOT REMARKABLE: 10 1 0 1 0 0 MUSCLE, SKELETAL (SM) ...... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 NERVE, SCIATIC (SN) ........ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 HEART (HT) .................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 LN, MESENTERIC (MS) ........ --EDEMA NUMBER EXAMINED: 10 NOT REMARKABLE: 9 1 0 0 0 3633 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 O F A N I M A L S A F F E C T E D -- SPLEEN (SP) ................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 THYMUS (TH) ................. --CYST ESOPHAGUS (ES) .............. NUMBER EXAMINED: 10 NOT REMARKABLE: 9 1 NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 0 0 0 TRACHEA (TR) ................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 ADRENAL, CORTEX (AC) --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --HEMORRHAGE --ONE EXAMINED NUMBER EXAMINED: 10 NOT REMARKABLE: 3 6 3 6 1 0 0 0 0 0 0 3634 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 T E D -- ADRENAL, MEDULLA (MA) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 8 --ONE EXAMINED --B-PHEOCHROMOCYTOMA PITUITARY (PI) ........................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 6 --HEMORRHAGE --PIGMENT --B-ADENOMA HARDERIAN GLAND (HG) .................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 9 --INFLAMMATION, CHRONIC 1 SALIV GL, MANDIB (SG) ................................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 STOMACH, GL (ST) --EDEMA NUMBER EXAMINED: 10 NOT REMARKABLE: 9 3635 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 O F A N I M A L S A F F E C T E D -- STOMACH, NONGL (SU) ........ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 DUODENUM (DU) ............... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 JEJUNUM (JE) ................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 ILEUM (IL) .................. NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 CECUM (CE) .................. --EDEMA COLON (CO) .................. NUMBER EXAMINED: 10 NOT REMARKABLE: 9 1 NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 0 0 0 3636 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION RECTUM (RE) ................ --PARASITISM URINARY BLADDER (UB) ...... -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALE- GROUP: -8- -9- NUMBER: 10 10 -=- -=- NUMBER EXAMINED: 10 NOT REMARKABLE: 8 0 0 20 NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 T E D -- UTERUS (UT) --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL --INFLAMMATION, ACUTE OVARY (OV) .......................... NUMBER EXAMINED: 10 NOT REMARKABLE: 8 1 1 1 NUMBER EXAMINED: 10 NOT REMARKABLE: 10 1 0 0 1 0 0 0 CERVIX (CV) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 VAGINA (VA) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 3637 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- NU] SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 A N I M A L S A F F E C T E D -- PROSTATE (PR) .................................. ...... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 SEMINAL VESICLES (SV) ......................... ...... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 EPIDIDYMIDES (EP) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 DEATH COMMENT (DC) ............................. ...... NUMBER EXAMINED: 10 NOT REMARKABLE: 0 --SCHEDULED SACRIFICE 10 LN, MANDIBULAR (MN) ............................ ...... NUMBER EXAMINED: NOT REMARKABLE: 2 0 --HEMORRHAGE 2 LN, AXILLARY (AX) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: 1 0 --HEMORRHAGE --INFILTRATE, MACROPHAGE, VACUOLATED 1 1 10 0 10 3 1 2 1 1 0 0 3638 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 FOOT/FOOTPAD (FP) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, INGUINAL (IN) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 AORTA (AO) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, RENAL (RL) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, LUMBAR/ILIAC (LM) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 HEMATO NEOPLASIA (HN) NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 MAMMARY, MALE (MM) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3639 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 CAVITY, ABDOM (PC) ......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 TAIL (TI) ................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 URETER (UE) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 CAVITY, ORAL (OC) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 CALVARIUM/SKULL (CA) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, MEDIASTINAL (ML) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, OTHER (LN) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3640 Covance 6329-212 TABLE 33 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 10 10 BILE DUCT (BD) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 AUDITORY SEB GL (AS) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 ADIPOSE TISSUE (AT) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 JOINT, OTHER (JT) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, POPLITEAL (PP) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 NASAL TURBINATE (NT) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3641 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ** TOP OF LIST ** LUNG (LU) .............................................. NUMBER EXAMINED: NOT REMARKABLE: 11 11 20 15 --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 02 03 01 KIDNEY (KD) ............................................ NUMBER EXAMINED: 11 20 NOT REMARKABLE: 0 0 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --MINERALIZATION, PELVIC --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --M-CARCINOMA, TUBULAR CELL 3 10 12 13 10 10 02 33 8 10 10 10 LIVER (LI) ............................................. NUMBER EXAMINED: 11 20 NOT REMARKABLE: 1 4 --DEGENERATION, CYSTIC --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC 24 01 01 3642 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ** FROM PREVIOUS PAGE ** LIVER (LI) .................... NUMBER EXAMINED: 11 20 NOT REMARKABLE: 1 4 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 16 11 45 6 10 05 11 02 PANCREAS (PA) ................. NUMBER EXAMINED: 11 20 NOT REMARKABLE: 3 10 --ATROPHY, ACINAR --FIBROSIS --HEMORRHAGE --HYPERPLASIA, ISLET CELLS --HYPERTROPHY, VASCULAR --INFLAMMATION, VASCULAR --PIGMENT --B-ADENOMA, ACINAR CELL --B-ADENOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 55 01 11 10 20 10 12 01 25 01 3643 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 11 20 THYROID (TY) ................................ ......... NUMBER EXAMINED: 11 NOT REMARKABLE: 5 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --FIBROSIS --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, CHRONIC --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 3 2 1 1 0 1 0 1 PARATHYROID (PT) ............................ ......... NUMBER EXAMINED: 11 NOT REMARKABLE: 10 --HYPERPLASIA 1 TESTIS (TE) ................................. ......... NUMBER EXAMINED: 11 NOT REMARKABLE: 6 --ATROPHY/DEGENERATION --CYST --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, VASCULAR --B-INTERSTITIAL CELL TUMOR 4 0 2 0 0 0 20 13 2 4 1 0 1 1 2 1 0 0 0 20 11 4 1 3 4 1 3 3644 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 11 20 SKIN (SK) ................................... ......... NUMBER EXAMINED: 11 NOT REMARKABLE: 7 --ATROPHY, ADNEXAL --CYST, EPIDERMAL INCLUSION --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --B-ADENOMA, BASAL CELL --B-FIBROMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL 0 1 0 0 1 2 0 1 0 1 1 MAMMARY, FEMALE (MF) ....................... ......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 4 0 1 1 2 1 0 1 1 1 2 0 0 0 0 BONE, FEMUR (FE) .................. ................... NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 MARROW, FEMUR (FM) ................. .................. NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 3645 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 11 20 MARROW, STERNUM (SE) NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 BONE, STERNUM (SB) NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 EYE (EY) .... --CATARACT BRAIN (BR) .. --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --INFLAMMATION, CHRONIC --MINERALIZATION SPINAL CORD (SC) NUMBER EXAMINED: 11 NOT REMARKABLE: 11 1 0 01 NUMBER EXAMINED: 11 NOT REMARKABLE: 4 2 0 42 30 10 20 NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 MUSCLE, SKELETAL (SM) NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 3646 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S SEX: --MALE -GROUP: -8- -9NUMBER: 11 20 NERVE, SCIATIC (SN) ................................... NUMBER EXAMINED: 10 NOT REMARKABLE: 10 0 0 HEART (HT) ............................................. NUMBER EXAMINED: 11 NOT REMARKABLE: 3 --DEGENERATION/NECROSIS --FIBROSIS 8 8 LN, MESENTERIC (MS) ................................... NUMBER EXAMINED: 11 NOT REMARKABLE: 11 --HEMORRHAGE --N-HEMANGIOSARCOMA 0 0 SPLEEN (SP) ............................................ NUMBER EXAMINED: 11 NOT REMARKABLE: 11 --B-LEIOMYOMA --M-HEMANGIOSARCOMA 0 0 THYMUS (TH) ............................................ NUMBER EXAMINED: 11 NOT REMARKABLE: 10 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1 0 0 0 0 2 1 1 1 3 1 1 1 0 0 0 Covance 6329-228 3M T-6316.1 PAGE: 6 A F F E C T E D -- 3647 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 11 20 ESOPHAGUS (ES) ........................ ............... NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 TRACHEA (TR) .......................... ............... NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 ADRENAL, CORTEX (AC) .................. ............... NUMBER EXAMINED: 11 NOT REMARKABLE: 4 --HEMORRHAGE --HYPERTROPHY --VACUOLATION 1 2 7 ADRENAL, MEDULLA (MA) ................. ............... NUMBER EXAMINED: 11 NOT REMARKABLE: 7 --ONE EXAMINED --B-PHEOCHROMOCYTOMA 1 4 PITUITARY (PI) ........................ ............... NUMBER EXAMINED: 11 NOT REMARKABLE: 1 --CYST --HEMORRHAGE --HYPERPLASIA --B-ADENOMA 0 3 1 9 0 0 0 0 0 0 0 0 0 9 0 1 7 0 9 3648 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 11 20 HARDERIAN GLAND (HG) ................. ................ NUMBER EXAMINED: 11 NOT REMARKABLE: 10 --INFLAMMATION, CHRONIC 1 SALIV GL, MANDIB (SG) ................ ................ NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 0 0 0 STOMACH, GL (ST) ..................... ................ NUMBER EXAMINED: 11 NOT REMARKABLE: 9 --EDEMA --EROSION --MINERALIZATION 1 1 1 STOMACH, NONGL (SU) .................. ................ NUMBER EXAMINED: 11 NOT REMARKABLE: 10 --EROSION --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --B-LEIOMYOMA --M-CARCINOMA, SQUAMOUS CELL 1 1 0 1 0 DUODENUM (DU) ........................ ................ NUMBER EXAMINED: 11 NOT REMARKABLE: 11 6 3 1 3 0 1 0 0 0 1 0 1 0 0 3649 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: --MALE-GROUP: -8- -9NUMBER: 11 20 O F A N I M A L S A F F E C T E D -- JEJUNUM (JE) NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 ILEUM (IL) NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 CECUM (CE) NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 COLON (CO) NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 RECTUM (RE) NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 URINARY BLADDER (UB) --CALCULUS --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC NUMBER EXAMINED: 11 NOT REMARKABLE: 9 0 2 1 20 7 13 0 0 3650 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 11 20 T E D -- UTERUS (UT) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 OVARY (OV) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 CERVIX (CV) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 VAGINA (VA) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 PROSTATE (PR) --EDEMA --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE SEMINAL VESICLES (SV) .......... --INFLAMMATION, CHRONIC NUMBER EXAMINED: 11 NOT REMARKABLE: 2 1 3 2 4 NUMBER EXAMINED: 11 NOT REMARKABLE: 9 1 0 0 0 0 0 0 0 0 0 3651 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ** FROM PREVIOUS PAGE ** SEMINAL VESICLES (SV) ......................... ...... NUMBER EXAMINED: NOT REMARKABLE: 11 9 0 0 --INFLAMMATION, CHRONIC-ACTIVE 10 EPIDIDYMIDES (EP) .............................. ...... NUMBER EXAMINED: 11 NOT REMARKABLE: 11 0 0 T E D -- DEATH COMMENT (DC) ............................. ...... NUMBER EXAMINED: 11 NOT REMARKABLE: 0 --SCHEDULED SACRIFICE 11 LN, MANDIBULAR (MN) ............................ ...... NUMBER EXAMINED: NOT REMARKABLE: 0 0 20 0 20 0 0 LN, AXILLARY (AX) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: --HEMORRHAGE --INFILTRATE, MACROPHAGE, VACUOLATED FOOT/FOOTPAD (FP) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: --EROSION 0 0 0 0 3 0 2 2 1 1 1 2 0 0 3652 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9 ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ** FROM PREVIOUS PAGE ** FOOT/FOOTPAD (FP) ..................................... NUMBER EXAMINED: NOT REMARKABLE: 3 0 2 0 --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE LN, INGUINAL (IN) ..................................... NUMBER EXAMINED: NOT REMARKABLE: T E D -- AORTA (AO) ............................................. NUMBER EXAMINED: NOT REMARKABLE: LN, RENAL (RL) ........................................ NUMBER EXAMINED: NOT REMARKABLE: --DILATATION, --FIBROSIS --HEMORRHAGE --INFILTRATE, SINUSOIDAL MACROPHAGE, PIGMENTED 11 01 11 10 LN, LUMBAR/ILIAC (LM) ................................. NUMBER EXAMINED: NOT REMARKABLE: 2 1 1 0 --DILATATION, SINUSOIDAL 10 3653 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 11 20 ** FROM PREVIOUS PAGE ** LN, LUMBAR/ILIAC (LM) . NUMBER EXAMINED: NOT REMARKABLE: 2 1 1 0 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 HEMATO NEOPLASIA (HN) ................................. NUMBER EXAMINED: 11 NOT REMARKABLE: 10 2 0 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 10 02 MAMMARY, MALE (MM) ....... NUMBER EXAMINED: NOT REMARKABLE: 5 0 0 0 --GALACTOCELE --MINERALIZATION --B-FIBROADENOMA 50 10 10 CAVITY, ABDOM (PC) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 TAIL (TI) --CYST, EPIDERMAL INCLUSION --B-PAPILLOMA, SQUAMOUS CELL NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 2 0 1 1 Covance 6329-228 3M T-6316.1 PAGE: 13 A F F E C T E D -- 3654 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: --MALE- GROUP: -8- -9NUMBER: 11 20 O F A N I M A L S A F F E C T E D -- URETER (UE) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 CAVITY, ORAL (OC) .................... NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 CALVARIUM/SKULL (CA) ................. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 LN, MEDIASTINAL (ML) ................. --DILATATION, SINUSOIDAL LN, OTHER (LN) ....................... --INFILTRATE, MACROPHAGE, PIGMENTED BILE DUCT (BD) ....................... NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 10 00 10 11 10 01 01 01 3655 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 11 20 T E D -- AUDITORY SEB GL (AS) .................................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) ................................. NUMBER EXAMINED: NOT REMARKABLE: --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ADIPOSE TISSUE (AT) ................................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 1 0 1 1 1 0 0 JOINT, OTHER (JT) ..................................... NUMBER EXAMINED: NOT REMARKABLE: --EDEMA --EXOSTOSES --FIBROSIS --INFLAMMATION, ACUTE, SYNOVIAL --INFLAMMATION, CHRONIC-ACTIVE, SYNOVIAL LN, POPLITEAL (PP) .................................... NUMBER EXAMINED: NOT REMARKABLE: 2 0 2 2 1 1 1 0 0 0 0 0 0 0 0 0 0 0 3656 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 11 20 NASAL TURBINATE (NT) ..................... ............ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3657 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ** TOP OF LIST ** LUNG (LU) ......................................... .... NUMBER EXAMINED: NOT REMARKABLE: 13 8 15 10 --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --MINERALIZATION, VASCULAR 10 11 10 01 23 KIDNEY (KD) ....................................... ... NUMBER EXAMINED: 13 15 NOT REMARKABLE: 0 0 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS 57 01 01 01 7 10 10 44 01 LIVER (LI) ........................................ ... NUMBER EXAMINED: 13 15 NOT REMARKABLE: 7 3 --CYST, BILIARY --DEGENERATION, CYSTIC --FIBROSIS --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC 10 11 10 12 T E D -- 3658 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ** FROM PREVIOUS PAGE ** LIVER (LI) ......................................... ... NUMBER EXAMINED: NOT REMARKABLE: 13 7 15 3 --FOCUS, ALTERED HEPATOCELLULAR, --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR EOSINOPHILIC 35 22 44 10 32 01 21 10 PANCREAS (PA) ...................................... ... NUMBER EXAMINED: 13 15 NOT REMARKABLE: 8 13 --ATROPHY, ACINAR --HEMORRHAGE --B-ADENOMA, ISLET CELL 21 20 31 THYROID (TY) ....................................... ... NUMBER EXAMINED: 13 15 NOT REMARKABLE: 7 8 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HYPERPLASIA, C-CELL --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 10 52 02 43 01 T E D -- 3659 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 T E D -- PARATHYROID (PT) .................. .................... NUMBER EXAMINED: 13 NOT REMARKABLE: 11 --HYPERPLASIA 2 TESTIS (TE) ........................ ................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 1 1 0 0 0 SKIN (SK) ................................... ......... NUMBER EXAMINED: 13 NOT REMARKABLE: 11 --CYST, EPIDERMAL INCLUSION --FIBROSIS --INFLAMMATION, CHRONIC-ACTIVE --B-KERATOACANTHOMA --B-PAPILLOMA, SQUAMOUS CELL --B-ADENOMA, SEBACEOUS GLAND 0 1 0 0 0 1 MAMMARY, FEMALE (MF) ....................... ......... NUMBER EXAMINED: 13 NOT REMARKABLE: 3 --FIBROSIS --GALACTOCELE --HEMORRHAGE --INFLAMMATION, ACUTE --MINERALIZATION --PIGMENT 1 9 4 1 3 1 3 0 1 0 1 1 1 0 15 3 0 10 0 0 2 0 3660 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ** FROM PREVIOUS PAGE ** MAMMARY, FEMALE (MF) .................................. NUMBER EXAMINED: NOT REMARKABLE: 13 15 33 --B-ADENOMA --B-FIBROADENOMA --M-CARCINOMA 11 66 61 BONE, FEMUR (FE) ...................................... NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 MARROW, FEMUR (FM) NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 MARROW, STERNUM (SE) NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 BONE, STERNUM (SB) NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 EYE (EY) --FIBROSIS, CORNEAL --INFLAMMATION, ACUTE, CORNEAL NUMBER EXAMINED: 13 NOT REMARKABLE: 11 0 0 10 20 3661 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ** FROM PREVIOUS PAGE ** EYE (EY) .................................. ............ NUMBER EXAMINED: NOT REMARKABLE: 13 11 0 0 --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 10 10 BRAIN (BR) ................................ ............ NUMBER EXAMINED: 13 10 NOT REMARKABLE: 7 0 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --INFLAMMATION, CHRONIC 5 10 42 10 SPINAL CORD (SC) ......................... ............ NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 MUSCLE, SKELETAL (SM) .................... ............ NUMBER EXAMINED: 13 NOT REMARKABLE: 13 --I-LEIOMYOSARCOMA 0 NERVE, SCIATIC (SN) ...................... ............ NUMBER EXAMINED: 13 NOT REMARKABLE: 13 1 0 1 0 0 3662 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 HEART (HT) ........................................ .... NUMBER EXAMINED: 13 NOT REMARKABLE: 9 --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC --METAPLASIA, CARTILAGE 3 2 1 1 LN, MESENTERIC (MS) ............................... ... NUMBER EXAMINED: 13 NOT REMARKABLE: 11 --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 1 2 SPLEEN (SP) ....................................... ... NUMBER EXAMINED: 13 NOT REMARKABLE: 12 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED 1 THYMUS (TH) ....................................... ... NUMBER EXAMINED: 13 NOT REMARKABLE: 9 --CYST --M-THYMIC CARCINOMA 3 1 ESOPHAGUS (ES) .................................... ... NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 3663 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 TRACHEA (TR) .................... ..................... NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 ADRENAL, CORTEX (AC) ........................ ........ NUMBER EXAMINED: 13 NOT REMARKABLE: 1 --ANGIECTASIS --DEGENERATION, CYSTIC, --HEMORRHAGE --MINERALIZATION --PIGMENT --THROMBUS --VACUOLATION --B-ADENOMA CORTICAL 8 5 12 1 2 4 2 2 ADRENAL, MEDULLA (MA) ....................... ........ NUMBER EXAMINED: 13 NOT REMARKABLE: 13 --NECROSIS, MEDULLARY --B-PHEOCHROMOCYTOMA 0 0 PITUITARY (PI) ............................... ........ NUMBER EXAMINED: 13 NOT REMARKABLE: 1 --CYST --HEMORRHAGE --PIGMENT 0 10 1 4 0 3 1 4 0 2 1 0 0 1 0 1 1 12 0 1 11 2 3664 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 24 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 13 15 ** FROM PREVIOUS PAGE ** PITUITARY (PI) ........................................ NUMBER EXAMINED: 13 NOT REMARKABLE: 1 12 0 --B-ADENOMA 12 12 HARDERIAN GLAND (HG) .................................. NUMBER EXAMINED: 13 NOT REMARKABLE: 8 0 0 --HYPERPLASIA, GLANDULAR --INFLAMMATION, CHRONIC 10 40 SALIV GL, MANDIB (SG) NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 STOMACH, GL (ST) --EDEMA --EROSION STOMACH, NONGL (SU) NUMBER EXAMINED: 13 NOT REMARKABLE: 12 0 1 NUMBER EXAMINED: 13 NOT REMARKABLE: 13 1 0 1 0 0 0 DUODENUM (DU) NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 3665 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 25 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 O F A N I M A L S A F F E C T E D -- JEJUNUM (JE) NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 ILEUM (IL) NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 CECUM (CE) NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 COLON (CO) NUMBER EXAMINED: 13 NOT REMARKABLE: 13 0 0 RECTUM (RE) --PARASITISM --M-LEIOMYOSARCOMA URINARY BLADDER (UB) NUMBER EXAMINED: 13 NOT REMARKABLE: 8 5 0 NUMBER EXAMINED: 13 NOT REMARKABLE: 13 1 0 0 1 15 15 3666 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 26 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 UTERUS (UT) ............................................ NUMBER EXAMINED: 13 NOT REMARKABLE: 9 --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL --B-POLYP, ENDOMETRIAL, STROMAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3 0 1 1 OVARY (OV) ............................................. NUMBER EXAMINED: 13 NOT REMARKABLE: 9 --CYST --B-GRANULOSA/THECA CELL TUMOR 4 1 CERVIX (CV) ............................................ NUMBER EXAMINED: 13 NOT REMARKABLE: 12 --HYPERTROPHY, MUSCULAR --B-LEIOMYOMA 0 1 VAGINA (VA) ............................................ NUMBER EXAMINED: 13 NOT REMARKABLE: 13 2 0 2 1 0 0 5 3 2 0 1 0 1 0 0 0 PROSTATE (PR) ......................................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3667 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 27 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 SEMINAL VESICLES (SV) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 EPIDIDYMIDES (EP) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 DEATH COMMENT (DC) ............................. ...... NUMBER EXAMINED: 13 NOT REMARKABLE: 0 --SCHEDULED SACRIFICE 13 LN, MANDIBULAR (MN) ............................ ...... NUMBER EXAMINED: NOT REMARKABLE: 2 0 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 1 1 LN, AXILLARY (AX) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: 4 4 --HEMORRHAGE --INFILTRATE, MACROPHAGE, VACUOLATED 0 0 15 0 15 2 1 0 1 3 1 2 1 3668 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 28 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 FOOT/FOOTPAD (FP) .......................... ......... NUMBER EXAMINED: NOT REMARKABLE: --CYST --EROSION --EXOSTOSES --FIBROSIS --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE --PIGMENT LN, INGUINAL (IN) .......................... ......... NUMBER EXAMINED: NOT REMARKABLE: --HYPERPLASIA, LYMPHOCYTIC AORTA (AO) .................................. ......... NUMBER EXAMINED: NOT REMARKABLE: 2 0 0 2 0 2 2 0 0 1 0 1 0 0 3 0 1 2 1 1 1 1 1 0 0 0 0 0 LN, RENAL (RL) .............................. ......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, LUMBAR/ILIAC (LM) ...................... ......... NUMBER EXAMINED: NOT REMARKABLE: 1 1 2 2 3669 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 HEMATO NEOPLASIA (HN) .... --M-SARCOMA, HISTIOCYTIC MAMMARY, MALE (MM) ....... NUMBER EXAMINED: 13 NOT REMARKABLE: 12 1 NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 CAVITY, ABDOM (PC) ....... NUMBER EXAMINED: NOT REMARKABLE: --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) TAIL (TI) .............................................. NUMBER EXAMINED: NOT REMARKABLE: --EROSION --INFLAMMATION, ACUTE --M-FIBROSARCOMA URETER (UE) ............................................ NUMBER EXAMINED: NOT REMARKABLE: 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 CAVITY, ORAL (OC) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 Covance 6329-228 3M T-6316.1 PAGE: 29 A F F E C T E D -- 3670 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 30 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 CALVARIUM/SKULL (CA) ....... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, MEDIASTINAL (ML) ....... --HEMORRHAGE LN, OTHER (LN) .............. NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 2 0 2 0 0 0 0 0 0 0 BILE DUCT (BD) .............. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 AUDITORY SEB GL (AS) ....... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) ...... NUMBER EXAMINED: NOT REMARKABLE: 1 1 0 0 3671 Covance 6329-212 TABLE 34 Incidence of Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 31 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 13 15 T E D -- ADIPOSE TISSUE (AT) ......................... ........ NUMBER EXAMINED: NOT REMARKABLE: --M-LIPOSARCOMA JOINT, OTHER (JT) ............................ ........ NUMBER EXAMINED: NOT REMARKABLE: --EXOSTOSES --FIBROSIS --INFLAMMATION, CHRONIC, SYNOVIAL LN, POPLITEAL (PP) .......................... ........ NUMBER EXAMINED: NOT REMARKABLE: 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 NASAL TURBINATE (NT) ........................ ........ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3672 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ** TOP OF LIST ** LUNG (LU) .............................................. NUMBER EXAMINED: NOT REMARKABLE: 39 31 30 20 --FIBROSIS, PLEURAL/SUBPLEURAL --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --N-TRANSITIONAL CELL CARCINOMA FOR TYPE) 11 23 31 01 01 35 11 10 KIDNEY (KD) ............................................ NUMBER EXAMINED: 39 30 NOT REMARKABLE: 2 3 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS --THROMBUS --I-TRANSITIONAL CELL CARCINOMA 12 5 21 85 01 21 02 11 32 12 23 19 60 40 10 3673 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 39 30 LIVER (LI) ......................... NUMBER EXAMINED: 39 NOT REMARKABLE: 14 --CYST --DEGENERATION, CYSTIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFILTRATE, MAST CELLS --INFLAMMATION, CHRONIC --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2 3 3 2 2 3 0 10 1 1 14 4 1 PANCREAS (PA) ...................... NUMBER EXAMINED: 39 NOT REMARKABLE: 28 --ATROPHY, ACINAR --HEMORRHAGE --HYPERTROPHY, VASCULAR --INFLAMMATION, VASCULAR --PIGMENT --THROMBUS --B-ADENOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 4 0 1 0 6 1 2 1 30 15 0 4 3 1 1 3 1 3 1 3 3 2 2 30 19 2 1 1 1 4 1 5 1 3674 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 39 30 THYROID (TY) ................................ ......... NUMBER EXAMINED: 39 NOT REMARKABLE: 29 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 5 4 0 1 2 0 PARATHYROID (PT) ............................ ......... NUMBER EXAMINED: 38 NOT REMARKABLE: 32 --HYPERPLASIA 6 TESTIS (TE) ................................. ......... NUMBER EXAMINED: 39 NOT REMARKABLE: 33 --ATROPHY/DEGENERATION --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, VASCULAR --SPERMATOCELE 3 2 3 0 1 SKIN (SK) ................................... ......... NUMBER EXAMINED: 39 NOT REMARKABLE: 32 --ATROPHY, ADNEXAL 0 30 19 4 4 1 1 1 1 4 2 2 25 21 3 1 0 1 0 11 2 1 3675 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ** FROM PREVIOUS PAGE ** SKIN (SK) ............. NUMBER EXAMINED: 39 11 NOT REMARKABLE: 32 2 --CYST, EPIDERMAL INCLUSION --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, ACUTE --B-BASAL CELL TUMOR --B-FIBROMA --B-KERATOACANTHOMA --M-CARCINOMA, SQUAMOUS CELL --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 11 11 03 11 15 10 30 02 01 01 11 MAMMARY, FEMALE (MF) .................................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 BONE, FEMUR (FE) --HEMORRHAGE --INFLAMMATION, CHRONIC MARROW, FEMUR (FM) ...... NUMBER EXAMINED: 39 NOT REMARKABLE: 38 1 1 NUMBER EXAMINED: 39 NOT REMARKABLE: 39 0 0 0 0 0 0 Covance 6329-228 3M T-6316.1 PAGE: 4 A F F E C T E D -- 3676 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -GROUP: -8- -9NUMBER: 39 30 MARROW, STERNUM (SE) ....................... ......... NUMBER EXAMINED: 39 NOT REMARKABLE: 39 0 0 BONE, STERNUM (SB) ......................... ......... NUMBER EXAMINED: 39 NOT REMARKABLE: 39 0 0 EYE (EY) .................................... ......... NUMBER EXAMINED: 39 NOT REMARKABLE: 35 --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, ACUTE, CILIARY --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 4 2 1 2 BRAIN (BR) .................................. ......... NUMBER EXAMINED: 39 NOT REMARKABLE: 20 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE 19 14 2 SPINAL CORD (SC) ............................ ......... NUMBER EXAMINED: 39 NOT REMARKABLE: 38 --HEMORRHAGE 1 1 1 0 0 0 0 16 2 14 7 0 0 0 0 3677 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D SEX: --MALE- GROUP: -8- -9NUMBER: 39 30 MUSCLE, SKELETAL (SM) ................................. NUMBER EXAMINED: 39 NOT REMARKABLE: 39 --I-SQUAMOUS CELL CARCINOMA 0 NERVE, SCIATIC (SN) ................................... NUMBER EXAMINED: 39 NOT REMARKABLE: 39 1 HEART (HT) --DEGENERATION/NECROSIS --FIBROSIS --MINERALIZATION --THROMBUS, ATRIAL LN, MESENTERIC (MS) ...... --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED EN (SP) ............................ --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED NUMBER EXAMINED: 39 NOT REMARKABLE: 17 21 14 1 2 NUMBER EXAMINED: 38 NOT REMARKABLE: 34 1 2 2 NUMBER EXAMINED: 39 NOT REMARKABLE: 36 2 2 3678 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ** FROM PREVIOUS PAGE ** SPLEEN (SP) ........................ NUMBER EXAMINED: 39 NOT REMARKABLE: 36 4 1 --THROMBUS --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 10 THYMUS (TH) ........................ NUMBER EXAMINED: 38 NOT REMARKABLE: 34 1 0 --CYST --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 30 20 01 ESOPHAGUS (ES) ..................... NUMBER EXAMINED: 39 NOT REMARKABLE: 39 0 0 TRACHEA (TR) ....................... NUMBER EXAMINED: 39 NOT REMARKABLE: 39 0 0 ADRENAL, CORTEX (AC) ............... --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE NUMBER EXAMINED: 39 NOT REMARKABLE: 17 1 5 0 6 6 1 0 1 1 2 3679 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ................. ................ NUMBER EXAMINED: NOT REMARKABLE: 39 17 6 1 --HYPERTROPHY --ONE EXAMINED --THROMBUS --VACUOLATION 21 20 10 13 3 ADRENAL, MEDULLA (MA) ................ ................ NUMBER EXAMINED: 39 NOT REMARKABLE: 33 3 0 --ONE EXAMINED --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --B-PHEOCHROMOCYTOMA 20 01 01 43 PITUITARY (PI) ....................... ................ NUMBER EXAMINED: 39 17 NOT REMARKABLE: 8 0 --CYST --HEMORRHAGE --INFLAMMATION, --PIGMENT --B-ADENOMA CHRONIC 12 14 14 10 02 30 17 3680 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S SEX: --MALE-GROUP: -8- -9NUMBER: 39 30 HARDERIAN GLAND (HG) --INFLAMMATION, CHRONIC SALIV GL, MANDIB (SG) .... NUMBER EXAMINED: 39 NOT REMARKABLE: 35 4 NUMBER EXAMINED: 39 NOT REMARKABLE: 39 0 0 0 0 0 STOMACH, GL (ST) NUMBER EXAMINED: 39 NOT REMARKABLE: 34 --EDEMA --EROSION --INFLAMMATION, ACUTE --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2 3 1 1 0 STOMACH, NONGL (SU) ................................... NUMBER EXAMINED: 39 NOT REMARKABLE: 28 --ACANTHOSIS --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR MUCOSA --INFLAMMATION, ACUTE 1 9 2 1 1 1 5 11 6 0 3 0 1 1 3 1 0 2 1 0 1 0 2 Covance 6329-228 3M T-6316.1 PAGE: 9 A F F E C T E D -- 3681 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 39 30 ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) ........................ ......... NUMBER EXAMINED: NOT REMARKABLE: 39 28 3 1 --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION 10 21 DUODENUM (DU) ............................... ......... NUMBER EXAMINED: 38 NOT REMARKABLE: 37 0 0 --HEMORRHAGE --INFLAMMATION, ACUTE --ULCERATION 10 10 10 JEJUNUM (JE) ................................ ......... NUMBER EXAMINED: 34 NOT REMARKABLE: 33 0 0 --HEMORRHAGE --INFLAMMATION, ACUTE --ULCERATION 10 10 10 ILEUM (IL) .................................. ......... NUMBER EXAMINED: 32 NOT REMARKABLE: 31 0 0 --EROSION --INFLAMMATION, ACUTE 10 10 T E D -- 3682 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 39 30 T E D -- CECUM (CE) .................... .................. NUMBER EXAMINED: 36 NOT REMARKABLE: 35 --EDEMA --INFLAMMATION, ACUTE --ULCERATION 1 1 1 COLON (CO) .................... .................. NUMBER EXAMINED: 38 NOT REMARKABLE: 36 --EDEMA --PARASITISM 1 1 RECTUM (RE) ................... .................. NUMBER EXAMINED: 38 NOT REMARKABLE: 37 --PARASITISM 1 URINARY BLADDER (UB) ......... .................. NUMBER EXAMINED: 39 NOT REMARKABLE: 33 --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1 4 1 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 0 0 0 3683 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION UTERUS (UT) -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-- GROUP: -8- -9- NUMBER: 39 30 -=- -=- NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 T E D -- OVARY (OV) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 CERVIX (CV) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 VAGINA (VA) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 PROSTATE (PR) NUMBER EXAMINED: 39 NOT REMARKABLE: 16 --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE --I-TRANSITIONAL CELL CARCINOMA "HEMATO NEOPLASIA" FOR TYPE) 0 6 16 0 1 10 1 1 1 7 1 0 3684 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 39 30 T E D -- SEMINAL VESICLES (SV) ......................... ...... NUMBER EXAMINED: 39 NOT REMARKABLE: 31 --EDEMA --FIBROSIS --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --SECRETION, INCREASED --M-TRANSITIONAL CELL CARCINOMA 1 1 2 2 1 1 2 1 EPIDIDYMIDES (EP) .............................. ...... NUMBER EXAMINED: 39 NOT REMARKABLE: 37 --GRANULOMA, SPERM --HYPOSPERMIA --INFLAMMATION, CHRONIC 1 1 0 DEATH COMMENT (DC) ............................. ...... NUMBER EXAMINED: 39 NOT REMARKABLE: 0 --ATRIAL THROMBUS --AUDITORY SEBACEOUS GLAND CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --CYSTITIS --ENTERITIS --FIBROMA 1 0 3 2 1 1 5 1 1 0 0 0 0 0 3 0 2 0 1 1 1 30 0 0 1 2 0 0 0 3685 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION ** FROM PREVIOUS PAGE ** DEATH COMMENT (DC) .... --FIBROSARCOMA --HEPATOCELLULAR ADENOMA --HEPATOCELLULAR NECROSIS --HISTIOCYTIC SARCOMA --MALIGNANT LYMPHOMA --MAMMARY FIBROADENOMA --OSTEOGENIC SARCOMA --PHEOCHROMOCYTOMA --PITUITARY ADENOMA --PYELONEPHRITIS --SQUAMOUS CELL CARCINOMA --TRANSITIONAL CELL CARCINOMA --UNDETERMINED LN, MANDIBULAR (MN) ............ --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC LN, AXILLARY (AX) .......... -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 39 30 T E D -- NUMBER EXAMINED: 39 NOT REMARKABLE: 0 0 2 1 1 0 1 0 0 18 1 0 1 6 NUMBER EXAMINED: NOT REMARKABLE: 4 2 2 0 NUMBER EXAMINED: NOT REMARKABLE: 0 0 30 0 1 1 0 2 1 0 1 1 14 0 1 0 5 4 2 1 2 0 0 3686 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -GROUP: -8- -9NUMBER: 39 30 T E D -- FOOT/FOOTPAD (FP) .......................... ......... NUMBER EXAMINED: NOT REMARKABLE: --CYST, KERATIN --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE LN, INGUINAL (IN) .......................... ......... NUMBER EXAMINED: NOT REMARKABLE: 8 0 1 3 6 1 3 1 7 1 0 0 4 1 0 1 2 0 2 0 3 0 0 0 AORTA (AO) .................................. ......... NUMBER EXAMINED: NOT REMARKABLE: --MINERALIZATION LN, RENAL (RL) .............................. ......... NUMBER EXAMINED: NOT REMARKABLE: --DILATATION, SINUSOIDAL --HEMORRHAGE 1 0 1 0 0 0 0 1 0 1 2 0 1 2 3687 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 39 30 T E D -- LN, LUMBAR/ILIAC (LM) ........ NUMBER EXAMINED: NOT REMARKABLE: 3 0 --DILATATION, SINUSOIDAL --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2 1 0 HEMATO NEOPLASIA (HN) ........ NUMBER EXAMINED: 39 NOT REMARKABLE: 37 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 1 1 MAMMARY, MALE (MM) ............ NUMBER EXAMINED: NOT REMARKABLE: 6 1 --GALACTOCELE --INFLAMMATION, CHRONIC --B-FIBROADENOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 4 1 1 0 CAVITY, ABDOM (PC) ............ NUMBER EXAMINED: NOT REMARKABLE: 0 0 6 1 4 0 1 3 0 1 2 5 3 1 0 0 1 0 0 3688 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE- GROUP: -8- -9NUMBER: 39 30 T E D -- TAIL (TI) NUMBER EXAMINED: NOT REMARKABLE: --HEMORRHAGE --INFLAMMATION, ACUTE --B-FIBROMA URETER (UE) ............................................ NUMBER EXAMINED: NOT REMARKABLE: --DILATATION CAVITY, ORAL (OC) ..................................... NUMBER EXAMINED: NOT REMARKABLE: CALVARIUM/SKULL (CA) .................................. NUMBER EXAMINED: NOT REMARKABLE: --HEMORRHAGE --M-SARCOMA, OSTEOGENIC 10 01 LN, MEDIASTINAL (ML) .................................. NUMBER EXAMINED: 1 1 NOT REMARKABLE: 0 1 --DILATATION, SINUSOIDAL --HEMORRHAGE 3689 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: --MALE-GROUP: -8- -9NUMBER: 39 30 O F A N I M A L S A F F E C T E D -- LN, OTHER (LN) ....................... --INFILTRATE, MACROPHAGE, PIGMENTED BILE DUCT (BD) ....................... --DILATATION AUDITORY SEB GL (AS) ................. --M-CARCINOMA LN, ANT MES/PANC (AP) ................ --DILATATION, SINUSOIDAL --INFILTRATE, MACROPHAGE, PIGMENTED ADIPOSE TISSUE (AT) .................. --FAT NECROSIS NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 1 1 0 1 1 0 1 1 1 0 1 3690 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE- GROUP: -8- -9NUMBER: 39 30 JOINT, OTHER (JT) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 LN, POPLITEAL (PP) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 NASAL TURBINATE (NT) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 3691 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ** TOP OF LIST ** LUNG (LU) ..................................... ...... NUMBER EXAMINED: NOT REMARKABLE: 37 31 35 24 --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --MINERALIZATION, VASCULAR 01 11 21 52 01 16 KIDNEY (KD) ................................... ...... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 2 4 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS 14 10 10 11 10 10 10 27 30 10 73 33 LIVER (LI) .................................... ...... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 19 17 --CYST, BILIARY 10 3692 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ** FROM PREVIOUS PAGE ** LIVER (LI) ......................... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 19 17 --DEGENERATION, CYSTIC --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --PIGMENT --TELANGIECTASIS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 01 02 11 12 34 42 12 10 20 12 7 11 01 PANCREAS (PA) ...................... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 36 30 --ATROPHY, ACINAR --HEMORRHAGE --B-ADENOMA, ISLET CELL 05 10 10 THYROID (TY) ....................... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 22 26 --CYST, ULTIMOBRANCHIAL 94 3693 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ** FROM PREVIOUS PAGE ** THYROID (TY) ................................ ......... NUMBER EXAMINED: NOT REMARKABLE: 37 22 35 26 --FIBROSIS --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, CHRONIC --MINERALIZATION --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 10 01 12 01 10 10 33 10 PARATHYROID (PT) ............................ ......... NUMBER EXAMINED: 35 NOT REMARKABLE: 34 0 0 --FIBROSIS 10 TESTIS (TE) ................................. ......... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 SKIN (SK) .............................................. NUMBER EXAMINED: 37 NOT REMARKABLE: 34 --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE 1 0 1 1 2 0 0 1 0 0 3694 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ** FROM PREVIOUS PAGE ** SKIN (SK) ................................... ......... NUMBER EXAMINED: NOT REMARKABLE: 37 34 2 0 --PIGMENT --B-BASAL CELL TUMOR --B-FIBROMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL 01 01 10 10 01 MAMMARY, FEMALE (MF) ....................... ......... NUMBER EXAMINED: 37 35 NOT REMARKABLE: 2 5 --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS --PIGMENT --B-ADENOMA --B-FIBROADENOMA --B-FIBROMA --M-CARCINOMA 35 27 24 13 21 01 58 20 32 41 14 15 10 38 BONE, FEMUR (FE) ............................ ......... NUMBER EXAMINED: 37 NOT REMARKABLE: 37 0 0 3695 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 24 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 MARROW, FEMUR (FM) ......... --HYPERCELLULARITY, MARROW MARROW, STERNUM (SE) ....... --HYPERCELLULARITY, MARROW BONE, STERNUM (SB) ......... NUMBER EXAMINED: 37 NOT REMARKABLE: 36 1 NUMBER EXAMINED: 37 NOT REMARKABLE: 36 1 NUMBER EXAMINED: 37 NOT REMARKABLE: 37 0 0 0 0 0 0 0 0 EYE (EY) ........................ --INFLAMMATION, ACUTE, CILIARY BRAIN (BR) ...................... --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --PIGMENT NUMBER EXAMINED: 37 NOT REMARKABLE: 36 1 NUMBER EXAMINED: 37 NOT REMARKABLE: 6 31 15 0 0 0 0 0 29 0 29 21 3 1 3696 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 25 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 O F A N I M A L S A F F E C T E D -- SPINAL CORD (SC) NUMBER EXAMINED: 37 NOT REMARKABLE: 37 1 1 MUSCLE, SKELETAL (SM) --MINERALIZATION NERVE, SCIATIC (SN) . NUMBER EXAMINED: 37 NOT REMARKABLE: 36 1 NUMBER EXAMINED: 37 NOT REMARKABLE: 37 1 1 0 1 1 HEART (HT) --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC LN, MESENTERIC (MS) ..... --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED NUMBER EXAMINED: 37 NOT REMARKABLE: 27 9 5 1 NUMBER EXAMINED: 37 NOT REMARKABLE: 34 2 2 2 1 0 1 1 2 2 0 0 3697 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 26 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 SPLEEN (SP) ....................................... .... NUMBER EXAMINED: 37 NOT REMARKABLE: 34 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED 2 1 THYMUS (TH) ....................................... ... NUMBER EXAMINED: 37 NOT REMARKABLE: 29 --CYST --M-FIBROSARCOMA 8 0 ESOPHAGUS (ES) .................................... ... NUMBER EXAMINED: 37 NOT REMARKABLE: 37 4 1 3 0 2 1 0 1 0 0 TRACHEA (TR) ...................................... ... NUMBER EXAMINED: 37 NOT REMARKABLE: 37 0 0 ADRENAL, CORTEX (AC) .............................. ... NUMBER EXAMINED: 37 NOT REMARKABLE: 8 --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY 22 11 1 26 1 8 0 5 8 1 8 0 3698 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 27 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 37 35 ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) .................... ............. NUMBER EXAMINED: NOT REMARKABLE: 37 8 8 0 --PIGMENT --THROMBUS --VACUOLATION 52 73 21 ADRENAL, MEDULLA (MA) ................... ............. NUMBER EXAMINED: 37 NOT REMARKABLE: 35 3 2 --HYPERPLASIA, MEDULLARY --B-PHEOCHROMOCYTOMA 10 11 PITUITARY (PI) .......................... ............. NUMBER EXAMINED: 36 31 NOT REMARKABLE: 2 0 --CYST --HEMORRHAGE --PIGMENT --B-ADENOMA --M-CARCINOMA 45 27 20 63 34 30 01 HARDERIAN GLAND (HG) .................... ............. NUMBER EXAMINED: 37 NOT REMARKABLE: 33 0 0 --INFLAMMATION, CHRONIC 40 3699 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 28 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 SALIV GL, MANDIB (SG) ................ ................ NUMBER EXAMINED: 37 NOT REMARKABLE: 37 0 0 STOMACH, GL (ST) ............................... ....... NUMBER EXAMINED: 37 NOT REMARKABLE: 36 --EROSION --INFLAMMATION, ACUTE 1 1 STOMACH, NONGL (SU) ............................. ..... NUMBER EXAMINED: 37 NOT REMARKABLE: 31 --ACANTHOSIS --EDEMA --EROSION --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR MUCOSA --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION 0 5 3 1 0 3 1 2 DUODENUM (DU) ................................... ..... NUMBER EXAMINED: 35 NOT REMARKABLE: 35 2 2 0 0 3 0 1 2 2 0 1 1 1 0 0 0 3700 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 JEJUNUM (JE) .......................................... NUMBER EXAMINED: 35 NOT REMARKABLE: 35 0 0 ILEUM (IL) ............................................. NUMBER EXAMINED: 32 NOT REMARKABLE: 32 0 0 CECUM (CE) ............................................. NUMBER EXAMINED: 37 NOT REMARKABLE: 37 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0 COLON (CO) ............................................. NUMBER EXAMINED: 37 NOT REMARKABLE: 37 3 1 2 0 0 RECTUM (RE) ............................................ NUMBER EXAMINED: 37 NOT REMARKABLE: 37 0 0 URINARY BLADDER (UB) .................................. NUMBER EXAMINED: 37 NOT REMARKABLE: 35 --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC 1 2 0 0 0 0 Covance 6329-228 3M T-6316.1 PAGE: 29 A F F E C T E D -- 3701 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 30 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 T E D -- UTERUS (UT) --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL --SCLEROSIS --B-POLYP, ENDOMETRIAL, STROMAL OVARY (OV) .......................... --CYST --INFLAMMATION, CHRONIC CERVIX (CV) .............. --DILATATION --INFLAMMATION, ACUTE --M-SARCOMA, ENDOMETRIAL STROMAL VAGINA (VA) ....................... --INFLAMMATION, ACUTE --N-SARCOMA, ENDOMETRIAL STROMAL NUMBER EXAMINED: 37 NOT REMARKABLE: 31 4 2 0 0 NUMBER EXAMINED: 37 NOT REMARKABLE: 32 4 1 NUMBER EXAMINED: 37 NOT REMARKABLE: 37 0 0 0 NUMBER EXAMINED: 37 NOT REMARKABLE: 36 1 0 7 0 3 4 1 2 7 1 6 0 1 0 1 1 1 8 7 1 1 3702 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 31 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 T E D -- PROSTATE (PR) .................... .................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 SEMINAL VESICLES (SV) ................ ................ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 EPIDIDYMIDES (EP) .................. .................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 DEATH COMMENT (DC) ......................... .......... NUMBER EXAMINED: 37 NOT REMARKABLE: 0 --ENDOMETRIAL STROMAL SARCOMA --FRACTURED CALVARIUM --HISTIOCYTIC SARCOMA --MALIGNANT LYMPHOMA --MAMMARY CARCINOMA --MAMMARY FIBROADENOMA --ORAL EROSION --OSTEOMA --PITUITARY ADENOMA --PITUITARY CARCINOMA --PYELONEPHRITIS --UNDETERMINED 0 1 0 0 0 3 1 1 29 0 1 1 35 0 1 0 1 1 3 2 0 0 25 1 0 1 3703 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 32 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 T E D -- LN, MANDIBULAR (MN) ............................ ...... NUMBER EXAMINED: NOT REMARKABLE: 8 4 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 2 2 1 LN, AXILLARY (AX) .............................. ...... NUMBER EXAMINED: 11 NOT REMARKABLE: 7 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, VACUOLATED 0 4 0 FOOT/FOOTPAD (FP) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: 0 0 --EDEMA --EROSION --EXOSTOSES --FIBROSIS --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE 0 0 0 0 0 0 LN, INGUINAL (IN) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: 2 1 --N-MAMMARY CARCINOMA 1 6 4 2 0 0 11 9 2 0 1 3 0 1 1 1 2 1 1 1 1 0 3704 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 33 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 AORTA (AO) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, RENAL (RL) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, LUMBAR/ILIAC (LM) .... NUMBER EXAMINED: NOT REMARKABLE: 0 0 2 2 HEMATO NEOPLASIA (HN) .... --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC MAMMARY, MALE (MM) ....... NUMBER EXAMINED: 37 NOT REMARKABLE: 37 0 0 NUMBER EXAMINED: NOT REMARKABLE: 0 0 2 0 1 1 0 0 CAVITY, ABDOM (PC) ....... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3705 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 34 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 T E D -- TAIL (TI) NUMBER EXAMINED: NOT REMARKABLE: URETER (UE) NUMBER EXAMINED: NOT REMARKABLE: CAVITY, ORAL (OC) --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, ACUTE CALVARIUM/SKULL (CA) .... --CYST, KERATIN --FRACTURE --HEMORRHAGE --INFLAMMATION, ACUTE LN, MEDIASTINAL (ML) .... --HEMORRHAGE NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 3706 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 35 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION ** FROM PREVIOUS PAGE ** LN, MEDIASTINAL (ML) .. --INFILTRATE, MACROPHAGE, PIGMENTED LN, OTHER (LN) ....................... -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 2 0 2 0 0 1 0 0 0 0 BILE DUCT (BD) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 AUDITORY SEB GL (AS) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, ANT MES/PANC (AP) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 ADIPOSE TISSUE (AT) --B-LIPOMA NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 1 0 1 3707 Covance 6329-212 TABLE 35 Incidence of Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 36 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 37 35 JOINT, OTHER (JT) ............. NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 LN, POPLITEAL (PP) ............ NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 NASAL TURBINATE (NT) ......... --B-OSTEOMA NUMBER EXAMINED: NOT REMARKABLE: 1 0 1 0 0 0 3708 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** TOP OF LIST ** LUNG (LU) .............................................. NUMBER EXAMINED: NOT REMARKABLE: 60 52 60 44 --FIBROSIS, PLEURAL/SUBPLEURAL --HEMORRHAGE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --MINERALIZATION, VASCULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" --N-TRANSITIONAL CELL CARCINOMA FOR TYPE) 11 01 23 34 01 01 38 12 10 KIDNEY (KD) ............................................ NUMBER EXAMINED: 60 60 NOT REMARKABLE: 7 4 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS 18 17 33 98 10 11 23 02 11 77 12 33 35 70 3709 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** KIDNEY (KD) ........................ NUMBER EXAMINED: 60 60 NOT REMARKABLE: 7 4 --THROMBUS --M-CARCINOMA, TUBULAR CELL --I-TRANSITIONAL CELL CARCINOMA 40 10 10 LIVER (LI) ......................... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 22 25 --CYST --DEGENERATION, CYSTIC --FIBROSIS, CAPSULAR --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFILTRATE, MAST CELLS --INFLAMMATION, CHRONIC --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) --NECROSIS, HEPATOCELLULAR --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 20 59 01 01 49 21 52 78 01 17 15 21 23 14 10 54 14 3710 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 PANCREAS (PA) ...................... .................. NUMBER EXAMINED: 60 NOT REMARKABLE: 41 --ATROPHY, ACINAR --FIBROSIS --HEMORRHAGE --HYPERPLASIA, ISLET CELLS --HYPERTROPHY, VASCULAR --INFLAMMATION, VASCULAR --PIGMENT --THROMBUS --B-ADENOMA, ACINAR CELL --B-ADENOMA, ISLET CELL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 9 0 1 1 3 1 7 1 0 4 1 THYROID (TY) ....................... .................. NUMBER EXAMINED: 60 NOT REMARKABLE: 42 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --FIBROSIS --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, CHRONIC --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 10 6 1 1 1 1 2 1 60 35 7 1 2 0 1 1 9 1 1 11 2 60 38 9 9 1 1 2 1 3 2 3711 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 PARATHYROID (PT) ............................ ......... NUMBER EXAMINED: 59 NOT REMARKABLE: 52 --HYPERPLASIA 7 TESTIS (TE) ................................. ......... NUMBER EXAMINED: 60 NOT REMARKABLE: 49 --ATROPHY/DEGENERATION --CYST --INFLAMMATION, CHRONIC --MINERALIZATION --MINERALIZATION, VASCULAR --SPERMATOCELE --B-INTERSTITIAL CELL TUMOR 7 0 4 3 0 1 0 SKIN (SK) ................................... ......... NUMBER EXAMINED: 60 NOT REMARKABLE: 49 --ATROPHY, ADNEXAL --CYST, EPIDERMAL INCLUSION --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --B-BASAL CELL TUMOR 0 2 1 0 0 2 3 0 1 4 2 2 55 41 8 1 4 4 2 0 3 15 2 2 2 1 5 1 1 6 1 0 3712 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** SKIN (SK) ............. NUMBER EXAMINED: 60 15 NOT REMARKABLE: 49 2 --B-ADENOMA, BASAL CELL --B-FIBROMA --B-KERATOACANTHOMA --B-PAPILLOMA, SQUAMOUS CELL --M-CARCINOMA, SQUAMOUS CELL --M-FIBROSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 11 32 02 10 11 01 11 MAMMARY, FEMALE (MF) .................................. NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 BONE, FEMUR (FE) --HEMORRHAGE --INFLAMMATION, CHRONIC MARROW, FEMUR (FM) ...... NUMBER EXAMINED: 60 NOT REMARKABLE: 59 1 1 NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 0 0 0 0 0 MARROW, STERNUM (SE) NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 0 Covance 6329-228 3M T-6316.1 PAGE: 5 A F F E C T E D -- 3713 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 -=- -=- BONE, STERNUM (SB) ................. .................. NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 0 EYE (EY) .................................... ......... NUMBER EXAMINED: 60 NOT REMARKABLE: 56 --CATARACT --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, ACUTE, CILIARY --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 0 4 2 1 2 BRAIN (BR) .................................. ......... NUMBER EXAMINED: 60 NOT REMARKABLE: 34 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --INFLAMMATION, CHRONIC --MINERALIZATION 23 17 2 1 2 SPINAL CORD (SC) ............................ ......... NUMBER EXAMINED: 60 NOT REMARKABLE: 59 --HEMORRHAGE 1 2 1 1 0 0 0 0 18 2 16 7 0 0 0 0 0 0 3714 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D SEX: --MALE- GROUP: -8- -9NUMBER: 70 70 MUSCLE, SKELETAL (SM) --I-SQUAMOUS CELL CARCINOMA NERVE, SCIATIC (SN) .......... NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 NUMBER EXAMINED: 59 NOT REMARKABLE: 59 1 HEART (HT) --DEGENERATION/NECROSIS --FIBROSIS --MINERALIZATION --THROMBUS, ATRIAL LN, MESENTERIC (MS) ...... --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --N-HEMANGIOSARCOMA NUMBER EXAMINED: 60 NOT REMARKABLE: 29 30 22 1 2 NUMBER EXAMINED: 59 NOT REMARKABLE: 55 3715 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -GROUP: -8- -9NUMBER: 70 70 SPLEEN (SP) ................... NUMBER EXAMINED: 60 NOT REMARKABLE: 57 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --THROMBUS --B-LEIOMYOMA --M-HEMANGIOSARCOMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2 0 0 0 1 THYMUS (TH) ................... NUMBER EXAMINED: 59 NOT REMARKABLE: 54 --CYST --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3 2 1 ESOPHAGUS (ES) ................ NUMBER EXAMINED: 60 NOT REMARKABLE: 60 8 3 2 1 1 1 0 1 0 0 0 1 0 0 TRACHEA (TR) .................. NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 0 ADRENAL, CORTEX (AC) ......... --ANGIECTASIS NUMBER EXAMINED: 60 NOT REMARKABLE: 31 1 6 1 0 3716 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) ........................ ........ NUMBER EXAMINED: NOT REMARKABLE: 60 31 6 1 --DEGENERATION, CYSTIC, CORTICAL --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY --ONE EXAMINED --THROMBUS --VACUOLATION 51 01 72 41 20 10 20 3 ADRENAL, MEDULLA (MA) ....................... ........ NUMBER EXAMINED: 60 NOT REMARKABLE: 50 3 0 --ONE EXAMINED --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --B-PHEOCHROMOCYTOMA 30 01 01 83 PITUITARY (PI) ............................... ........ NUMBER EXAMINED: 59 27 NOT REMARKABLE: 17 0 --CYST --HEMORRHAGE --HYPERPLASIA --INFLAMMATION, --PIGMENT --B-ADENOMA CHRONIC 13 17 22 10 10 02 40 27 3717 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 HARDERIAN GLAND (HG) --INFLAMMATION, CHRONIC --ONE EXAMINED SALIV GL, MANDIB (SG) .... NUMBER EXAMINED: 60 NOT REMARKABLE: 54 5 1 NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 0 0 0 0 0 STOMACH, GL (ST) NUMBER EXAMINED: 60 NOT REMARKABLE: 52 --EDEMA --EROSION --INFLAMMATION, ACUTE --MINERALIZATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 4 4 1 2 0 STOMACH, NONGL (SU) ................................... NUMBER EXAMINED: 60 NOT REMARKABLE: 48 --ACANTHOSIS --EDEMA --EROSION --FIBROSIS --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR MUCOSA 1 9 3 1 1 1 17 9 1 6 0 1 1 4 1 0 2 1 0 1 0 Covance 6329-228 3M T-6316.1 PAGE: 10 A F F E C T E D -- 3718 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION ** FROM PREVIOUS PAGE ** STOMACH, NONGL (SU) ..... --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC -ACTIVE --ULCERATION --B-LEIOMYOMA --M-CARCINOMA, SQUAMOUS CELL DUODENUM (DU) .... --HEMORRHAGE --INFLAMMATION, ACUTE --ULCERATION JEJUNUM (JE) .... --HEMORRHAGE --INFLAMMATION, ACUTE --ULCERATION ILEUM (IL) ...... --EROSION --INFLAMMATION, ACUTE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 T E D -- ......... NUMBER EXAMINED: 60 NOT REMARKABLE: 48 6 1 2 1 0 ......... NUMBER EXAMINED: 59 NOT REMARKABLE: 58 1 1 1 ......... NUMBER EXAMINED: 55 NOT REMARKABLE: 54 1 1 1 ......... NUMBER EXAMINED: 53 NOT REMARKABLE: 52 1 1 4 1 2 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3719 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE -GROUP: -8- -9NUMBER: 70 70 T E D -- CECUM (CE) ......................... .................. NUMBER EXAMINED: 57 NOT REMARKABLE: 56 --EDEMA --INFLAMMATION, ACUTE --ULCERATION 1 1 1 COLON (CO) ......................... .................. NUMBER EXAMINED: 59 NOT REMARKABLE: 57 --EDEMA --PARASITISM 1 1 RECTUM (RE) ........................ .................. NUMBER EXAMINED: 59 NOT REMARKABLE: 57 --PARASITISM 2 URINARY BLADDER (UB) ............... .................. NUMBER EXAMINED: 60 NOT REMARKABLE: 52 --CALCULUS --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 0 1 6 2 2 1 1 1 1 0 0 0 0 0 0 0 0 0 0 22 8 13 0 1 1 0 0 0 3720 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 T E D -- UTERUS (UT) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 OVARY (OV) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 CERVIX (CV) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 VAGINA (VA) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 PROSTATE (PR) ......................................... NUMBER EXAMINED: 60 NOT REMARKABLE: 26 --EDEMA --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --X-HEMATOPOIETIC NEOPLASIA (SEE --I-TRANSITIONAL CELL CARCINOMA "HEMATO NEOPLASIA" FOR TYPE) 1 0 10 2 21 0 1 10 1 0 1 1 0 7 1 0 3721 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 T E D -- SEMINAL VESICLES (SV) ......................... ...... NUMBER EXAMINED: 60 NOT REMARKABLE: 50 --EDEMA --FIBROSIS --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --SECRETION, INCREASED --M-TRANSITIONAL CELL CARCINOMA 1 1 2 3 2 1 2 1 EPIDIDYMIDES (EP) .............................. ...... NUMBER EXAMINED: 60 NOT REMARKABLE: 58 --GRANULOMA, SPERM --HYPOSPERMIA --INFLAMMATION, CHRONIC 1 1 0 DEATH COMMENT (DC) ............................. ...... NUMBER EXAMINED: 60 NOT REMARKABLE: 0 --ATRIAL THROMBUS --AUDITORY SEBACEOUS GLAND CARCINOMA --CHRONIC PROGRESSIVE NEPHROPATHY --CYSTITIS --ENTERITIS --FIBROMA 1 0 3 2 1 1 5 1 1 0 0 0 0 0 3 0 2 0 1 1 1 60 0 0 1 2 0 0 0 3722 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION ** FROM PREVIOUS PAGE ** DEATH COMMENT (DC) .... --FIBROSARCOMA --HEPATOCELLULAR ADENOMA --HEPATOCELLULAR NECROSIS --HISTIOCYTIC SARCOMA --MALIGNANT LYMPHOMA --MAMMARY FIBROADENOMA --OSTEOGENIC SARCOMA --PHEOCHROMOCYTOMA --PITUITARY ADENOMA --PYELONEPHRITIS --SCHEDULED SACRIFICE --SQUAMOUS CELL CARCINOMA --TRANSITIONAL CELL CARCINOMA --UNDETERMINED LN, MANDIBULAR (MN) ............ --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC LN, AXILLARY (AX) .......... --HEMORRHAGE -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 T E D -- NUMBER EXAMINED: 60 NOT REMARKABLE: 0 0 2 1 1 0 1 0 0 18 1 21 0 1 6 NUMBER EXAMINED: NOT REMARKABLE: 4 2 2 0 NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 60 0 1 1 0 2 1 0 1 1 14 0 30 1 0 5 4 2 1 2 2 1 1 3723 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION ** FROM PREVIOUS PAGE ** LN, AXILLARY (AX) .... --INFILTRATE, MACROPHAGE, VACUOLATED FOOT/FOOTPAD (FP) ..................... --CYST, KERATIN --EDEMA --EROSION --EXOSTOSES --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE LN, INGUINAL (IN) ............... -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 T E D -- NUMBER EXAMINED: NOT REMARKABLE: 0 0 2 1 01 NUMBER EXAMINED: 11 6 NOT REMARKABLE: 0 1 10 31 82 10 54 30 10 3 11 NUMBER EXAMINED: NOT REMARKABLE: 00 00 AORTA (AO) --MINERALIZATION NUMBER EXAMINED: NOT REMARKABLE: 12 01 11 3724 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 T E D -- LN, RENAL (RL) .. NUMBER EXAMINED: NOT REMARKABLE: 2 1 --DILATATION, SINUSOIDAL --FIBROSIS --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED 1 0 1 1 LN, LUMBAR/ILIAC (LM) ................................. NUMBER EXAMINED: NOT REMARKABLE: 5 1 --DILATATION, SINUSOIDAL --HEMORRHAGE --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 3 1 0 HEMATO NEOPLASIA (HN) ................................. NUMBER EXAMINED: 70 NOT REMARKABLE: 67 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 2 1 MAMMARY, MALE (MM) .................................... NUMBER EXAMINED: 11 NOT REMARKABLE: 1 --GALACTOCELE --INFLAMMATION , CHRONIC --MINERALIZATION --B-FIBROADENOMA 9 1 1 2 3 0 2 1 3 0 7 1 4 0 2 5 0 1 4 5 3 1 0 0 0 3725 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S SEX: --MALE -GROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** MAMMARY, MALE (MM) .... NUMBER EXAMINED: 11 NOT REMARKABLE: 1 5 3 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 01 CAVITY, ABDOM (PC) .................................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 TAIL (TI) --CYST, EPIDERMAL INCLUSION --HEMORRHAGE --INFLAMMATION, ACUTE --B-FIBROMA --B-PAPILLOMA, SQUAMOUS CELL URETER (UE) ................... --DILATATION CAVITY, ORAL (OC) NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 2 0 0 1 0 1 0 2 0 2 0 0 3 0 1 0 1 0 1 0 0 0 0 0 Covance 6329-228 3M T-6316.1 PAGE: 18 A F F E C T E D -- 3726 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 T E D -- CALVARIUM/SKULL (CA) ......................... ....... NUMBER EXAMINED: NOT REMARKABLE: --HEMORRHAGE --M-SARCOMA, 'OSTEOGENIC LN, MEDIASTINAL (ML) ......................... ...... NUMBER EXAMINED: NOT REMARKABLE: --DILATATION, SINUSOIDAL --HEMORRHAGE LN, OTHER (LN) . ...... NUMBER EXAMINED: NOT REMARKABLE: --INFILTRATE, MACROPHAGE, PIGMENTED BILE DUCT (BD) . ...... NUMBER EXAMINED: NOT REMARKABLE: --DILATATION AUDITORY SEB GL (AS) ......................... ...... NUMBER EXAMINED: NOT REMARKABLE: --M-CARCINOMA 1 0 1 0 2 0 2 1 2 2 0 0 0 0 0 0 0 1 0 0 1 1 1 0 0 2 0 2 2 1 1 1 0 1 3727 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE -GROUP: -8- -9NUMBER: 70 70 LN, ANT MES/PANC (AP) ................................. NUMBER EXAMINED: NOT REMARKABLE: --DILATATION, SINUSOIDAL --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ADIPOSE TISSUE (AT) ................................... NUMBER EXAMINED: NOT REMARKABLE: --FAT NECROSIS JOINT, OTHER (JT) ..................................... NUMBER EXAMINED: NOT REMARKABLE: --EDEMA --EXOSTOSES --FIBROSIS --INFLAMMATION, ACUTE, SYNOVIAL --INFLAMMATION, CHRONIC-ACTIVE, SYNOVIAL LN, POPLITEAL (PP) .................................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 2 0 2 2 1 1 1 0 0 2 0 1 1 2 1 1 0 1 0 0 0 0 0 0 0 0 0 3728 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-GROUP: -8- -9NUMBER: 70 70 NASAL TURBINATE (NT) ..................... ............ NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 3729 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 22 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** TOP OF LIST ** LUNG (LU) ..................................... . ...... NUMBER EXAMINED: NOT REMARKABLE: 60 46 60 43 --HEMORRHAGE --HYPERPLASIA, TYPE II PNEUMOCYTE --INFLAMMATION, CHRONIC --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, MACROPHAGE, PIGMENTED --INFLAMMATION, GRANULOMATOUS --MINERALIZATION --MINERALIZATION, VASCULAR 21 11 22 73 01 10 01 49 KIDNEY (KD) ................................... . ...... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 7 9 --CAST, PROTEINACEOUS --CYST --DILATATION, PELVIC --HEMORRHAGE --HYPERPLASIA, TRANSITIONAL CELL --INFARCT --INFLAMMATION, CHRONIC --MINERALIZATION, PELVIC --MINERALIZATION, TUBULAR --NEPHROPATHY, CHRONIC PROGRESSIVE --PYELONEPHRITIS 21 20 22 12 10 11 10 02 38 44 20 11 7 34 3730 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 LIVER (LI) ......................... NUMBER EXAMINED: 60 NOT REMARKABLE: 35 --CYST, BILIARY --DEGENERATION, CYSTIC --FIBROSIS --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERPLASIA, BILE DUCT --INFLAMMATION, CHRONIC --NECROSIS, HEPATOCELLULAR --PIGMENT --TELANGIECTASIS --THROMBUS --VACUOLATION, HEPATOCELLULAR --B-ADENOMA, HEPATOCELLULAR --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 2 1 1 1 3 1 2 5 8 3 1 5 0 14 2 0 PANCREAS (PA) ...................... NUMBER EXAMINED: 59 NOT REMARKABLE: 53 --ATROPHY, ACINAR --HEMORRHAGE --B-ADENOMA, ISLET CELL 2 3 4 60 29 0 2 0 3 7 1 2 7 6 2 0 2 1 8 1 1 60 51 8 0 1 3731 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 24 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 THYROID (TY) ....................... ................... NUMBER EXAMINED: 60 NOT REMARKABLE: 37 --CYST, FOLLICULAR --CYST, ULTIMOBRANCHIAL --FIBROSIS --HEMORRHAGE --HYPERPLASIA, C-CELL --HYPERPLASIA, FOLLICULAR CELL --INFLAMMATION, CHRONIC --MINERALIZATION --B-ADENOMA, C-CELL --B-ADENOMA, FOLLICULAR CELL 1 16 1 0 1 0 2 1 7 1 PARATHYROID (PT) ................... ................... NUMBER EXAMINED: 58 NOT REMARKABLE: 55 --FIBROSIS --HYPERPLASIA 1 2 TESTIS (TE) ........................ ................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 60 42 1 7 0 1 4 1 0 0 6 1 1 1 0 0 0 0 SKIN (SK) ................................. ............ NUMBER EXAMINED: 60 NOT REMARKABLE: 55 --CYST, EPIDERMAL INCLUSION 0 6 0 1 3732 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 25 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** SKIN (SK) ................................... ......... NUMBER EXAMINED: NOT REMARKABLE: 60 55 6 0 --EROSION --FIBROSIS --HEMORRHAGE --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --PIGMENT --B-BASAL CELL TUMOR --B-FIBROMA --B-KERATOACANTHOMA --B-PAPILLOMA, SQUAMOUS CELL --B-ADENOMA, SEBACEOUS GLAND --M-CARCINOMA, SQUAMOUS CELL 11 11 10 11 01 01 01 10 01 11 10 01 MAMMARY, FEMALE (MF) ....................... ......... NUMBER EXAMINED: 60 60 NOT REMARKABLE: 11 12 --FIBROSIS --GALACTOCELE --HEMORRHAGE --HYPERPLASIA, GLANDULAR --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --MINERALIZATION --NECROSIS --PIGMENT 11 47 43 65 13 31 01 9 11 20 42 3733 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 26 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** MAMMARY, FEMALE (MF) .................................. NUMBER EXAMINED: NOT REMARKABLE: 60 11 60 12 --B-ADENOMA --B-FIBROADENOMA --B-FIBROMA --M-CARCINOMA 52 21 22 10 99 BONE, FEMUR (FE) ...................................... NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 0 MARROW, FEMUR (FM) ......... --HYPERCELLULARITY, MARROW MARROW, STERNUM (SE) ....... --HYPERCELLULARITY, MARROW BONE, STERNUM (SB) ......... NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 60 0 59 0 10 60 0 59 0 10 60 0 60 0 3734 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 27 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 EYE (EY) .................................... ......... NUMBER EXAMINED: 60 NOT REMARKABLE: 57 --FIBROSIS, CORNEAL --INFLAMMATION, ACUTE, CORNEAL --INFLAMMATION, ACUTE, CILIARY --ULCERATION, CORNEAL --VASCULARIZATION, CORNEAL 1 2 1 1 1 BRAIN (BR) .................................. ......... NUMBER EXAMINED: 60 NOT REMARKABLE: 23 --COMPRESSION, VENTRAL --DILATATION, VENTRICULAR --HEMORRHAGE --INFLAMMATION, CHRONIC --PIGMENT 36 19 0 1 0 SPINAL CORD (SC) ............................ ......... NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 0 0 0 0 0 0 40 0 40 23 3 0 1 1 1 MUSCLE, SKELETAL (SM) ................ ................ NUMBER EXAMINED: 60 NOT REMARKABLE: 59 --MINERALIZATION --I-LEIOMYOSARCOMA 1 0 2 1 0 1 3735 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 28 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 NERVE, SCIATIC (SN) NUMBER EXAMINED: 60 NOT REMARKABLE: 60 HEART (HT) --DEGENERATION/NECROSIS --FIBROSIS --INFLAMMATION, CHRONIC --METAPLASIA, CARTILAGE LN, MESENTERIC (MS) ...... --EDEMA --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED SEN (SP) ............................ --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED --PIGMENT, INCREASED NUMBER EXAMINED: 60 NOT REMARKABLE: 46 12 7 2 1 NUMBER EXAMINED: 60 NOT REMARKABLE: 54 1 3 4 NUMBER EXAMINED: 60 NOT REMARKABLE: 56 3736 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 29 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 THYMUS (TH) ............................ ............... NUMBER EXAMINED: 60 NOT REMARKABLE: 47 --CYST --M-FIBROSARCOMA --M-THYMIC CARCINOMA 12 0 1 ESOPHAGUS (ES) ........................ ............... NUMBER EXAMINED: 60 NOT REMARKABLE: 60 2 1 0 1 0 0 0 TRACHEA (TR) .......................... ............... NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 0 ADRENAL, CORTEX (AC) ........................ ........ NUMBER EXAMINED: 60 NOT REMARKABLE: 12 --ANGIECTASIS --DEGENERATION, CYSTIC, CORTICAL --HEMATOPOIESIS, EXTRAMEDULLARY --HEMORRHAGE --HYPERTROPHY --MINERALIZATION --ONE EXAMINED --PIGMENT --THROMBUS --VACUOLATION 36 19 1 44 1 1 1 7 11 4 12 0 8 9 1 12 0 0 0 4 4 1 3737 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 30 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** ADRENAL, CORTEX (AC) .................... ............. NUMBER EXAMINED: NOT REMARKABLE: 60 12 12 0 --B-ADENOMA 20 ADRENAL, MEDULLA (MA) ................... ............. NUMBER EXAMINED: 60 NOT REMARKABLE: 56 4 2 --ONE EXAMINED --HYPERPLASIA, MEDULLARY --NECROSIS, MEDULLARY --B-PHEOCHROMOCYTOMA 10 10 01 22 PITUITARY (PI) .......................... ............. NUMBER EXAMINED: 59 45 NOT REMARKABLE: 9 0 --CYST --HEMORRHAGE --PIGMENT --B-ADENOMA --M-CARCINOMA 46 40 31 85 50 44 01 HARDERIAN GLAND (HG) .................... ............. NUMBER EXAMINED: 60 NOT REMARKABLE: 50 0 0 --HYPERPLASIA, GLANDULAR --INFLAMMATION, CHRONIC 10 90 T E D -- 3738 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 31 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 T E D -- SALIV GL, MANDIB (SG) ................ ................ NUMBER EXAMINED: 60 NOT REMARKABLE: 60 0 0 STOMACH, GL (ST) ............................... ....... NUMBER EXAMINED: 60 NOT REMARKABLE: 57 --EDEMA --EROSION --INFLAMMATION, ACUTE 1 2 1 STOMACH, NONGL (SU) ............................. ..... NUMBER EXAMINED: 60 NOT REMARKABLE: 54 --ACANTHOSIS --EDEMA --EROSION --HEMORRHAGE --HYPERKERATOSIS, NONGLANDULAR MUCOSA --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION 0 5 3 1 0 3 1 2 DUODENUM (DU) ................................... ..... NUMBER EXAMINED: 58 NOT REMARKABLE: 58 3 2 1 0 0 3 0 1 2 2 0 1 1 1 0 0 0 3739 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 JEJUNUM (JE) .......................................... NUMBER EXAMINED: 58 NOT REMARKABLE: 58 0 0 ILEUM (IL) ............................................. NUMBER EXAMINED: 55 NOT REMARKABLE: 55 0 0 CECUM (CE) ............................................. NUMBER EXAMINED: 60 NOT REMARKABLE: 59 --EDEMA --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 1 0 COLON (CO) ............................................. NUMBER EXAMINED: 60 NOT REMARKABLE: 60 3 1 0 2 0 0 RECTUM (RE) ............................................ NUMBER EXAMINED: 60 NOT REMARKABLE: 53 --PARASITISM --M-LEIOMYOSARCOMA 7 0 URINARY BLADDER (UB) .................................. NUMBER EXAMINED: 60 NOT REMARKABLE: 58 --HYPERPLASIA, TRANSITIONAL CELL 1 1 0 0 1 15 15 0 Covance 6329-228 3M T-6316.1 PAGE: 32 A F F E C T E D -- 3740 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 33 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** URINARY BLADDER (UB) .................................. NUMBER EXAMINED: NOT REMARKABLE: 60 58 15 15 --INFLAMMATION, CHRONIC 20 UTERUS (UT) ............................................ NUMBER EXAMINED: 60 10 NOT REMARKABLE: 48 0 --DILATATION --HYPERPLASIA, CYSTIC, ENDOMETRIAL --INFLAMMATION, ACUTE --SCLEROSIS --B-POLYP, ENDOMETRIAL, STROMAL --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) 85 36 10 01 12 10 OVARY (OV) ............................................. NUMBER EXAMINED: 60 12 NOT REMARKABLE: 51 4 --CYST --INFLAMMATION, CHRONIC --B-GRANULOSA/THECA CELL TUMOR 88 10 10 CERVIX (CV) ............................................ NUMBER EXAMINED: 60 NOT REMARKABLE: 59 2 0 --DILATATION --HYPERTROPHY, MUSCULAR --INFLAMMATION, ACUTE 01 01 01 T E D -- 3741 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 34 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** CERVIX (CV) ............................................ NUMBER EXAMINED: 60 NOT REMARKABLE: 59 2 0 --B-LEIOMYOMA --M-SARCOMA, ENDOMETRIAL STROMAL 10 01 VAGINA (VA) NUMBER EXAMINED: 60 NOT REMARKABLE: 59 8 7 --INFLAMMATION, ACUTE --N-SARCOMA, ENDOMETRIAL STROMAL 11 01 PROSTATE (PR) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 T E D -- SEMINAL VESICLES (SV) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 EPIDIDYMIDES (EP) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 DEATH COMMENT (DC) .................................... NUMBER EXAMINED: 60 NOT REMARKABLE: 0 --ENDOMETRIAL STROMAL SARCOMA 0 60 0 1 3742 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- NU] SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** DEATH COMMENT (DC) ............................ ....... NUMBER EXAMINED: NOT REMARKABLE: 60 0 60 0 --FRACTURED CALVARIUM --HISTIOCYTIC SARCOMA --MALIGNANT LYMPHOMA --MAMMARY CARCINOMA --MAMMARY FIBROADENOMA --ORAL EROSION --OSTEOMA --PITUITARY ADENOMA --PITUITARY CARCINOMA --PYELONEPHRITIS --SCHEDULED SACRIFICE --UNDETERMINED 10 01 01 03 32 10 10 29 25 01 10 23 25 11 LN, MANDIBULAR (MN) .......................... ....... NUMBER EXAMINED: 12 11 NOT REMARKABLE: 4 6 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED 54 31 10 LN, AXILLARY (AX) ............................. ....... NUMBER EXAMINED: 16 15 NOT REMARKABLE: 11 11 --HEMORRHAGE --HYPERPLASIA, LYMPHOCYTIC 14 40 3743 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 35 T E D -- Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 36 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9- ORGAN AND FINDING DESCRIPTION NUMBER: 70 70 ** FROM PREVIOUS PAGE ** LN, AXILLARY (AX) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: 16 11 15 11 --INFILTRATE, MACROPHAGE, VACUOLATED 12 FOOT/FOOTPAD (FP) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: 2 0 6 0 --CYST --EDEMA --EROSION --EXOSTOSES --FIBROSIS --INFLAMMATION, ACUTE --INFLAMMATION, CHRONIC ACTIVE --PIGMENT 01 01 23 02 23 22 02 01 LN, INGUINAL (IN) .............................. ...... NUMBER EXAMINED: NOT REMARKABLE: 3 1 1 1 --HYPERPLASIA, LYMPHOCYTIC --N-MAMMARY CARCINOMA 10 10 AORTA (AO) ..................................... ...... NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 T E D -- 3744 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 LN, RENAL (RL) NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 LN, LUMBAR/ILIAC (LM) NUMBER EXAMINED: NOT REMARKABLE: 1 1 4 4 HEMATO NEOPLASIA (HN) NUMBER EXAMINED: 70 NOT REMARKABLE: 69 --M-LYMPHOMA --M-SARCOMA, HISTIOCYTIC 0 1 MAMMARY, MALE (MM) .................................... NUMBER EXAMINED: NOT REMARKABLE: 0 0 2 0 1 1 0 0 CAVITY, ABDOM (PC) .................................... NUMBER EXAMINED: NOT REMARKABLE: --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) TAIL (TI) .............................................. NUMBER EXAMINED: NOT REMARKABLE: --EROSION --INFLAMMATION, ACUTE 1 0 1 0 0 0 0 0 0 0 1 0 1 1 Covance 6329-228 3M T-6316.1 PAGE: 37 A F F E C T E D -- 3745 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 38 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION ** FROM PREVIOUS PAGE ** TAIL (TI) .............. --M-FIBROSARCOMA URETER (UE) ....... -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 T E D -- NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: CAVITY, ORAL (OC) --EDEMA --EROSION --HEMORRHAGE --INFLAMMATION, ACUTE CALVARIUM/SKULL (CA) .... --CYST, KERATIN --FRACTURE --HEMORRHAGE --INFLAMMATION, ACUTE NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 3746 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 39 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 T E D -- LN, MEDIASTINAL (ML) --HEMORRHAGE --INFILTRATE, MACROPHAGE, PIGMENTED LN, OTHER (LN) ....................... NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 41 00 21 20 00 00 BILE DUCT (BD) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 AUDITORY SEB GL (AS) NUMBER EXAMINED: 0 NOT REMARKABLE: 0 0 0 LN, ANT MES/PANC (AP) NUMBER EXAMINED: 1 NOT REMARKABLE: 1 0 0 ADIPOSE TISSUE (AT) --B-LIPOMA --M-LIPOSARCOMA NUMBER EXAMINED: 1 NOT REMARKABLE: 0 0 1 1 0 1 0 3747 Covance 6329-212 TABLE 36 Incidence of Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 40 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R SEX: -FEMALEGROUP: -8- -9NUMBER: 70 70 O F A N I M A L S A F F E C T E D -- JOINT, OTHER (JT) --EXOSTOSES --FIBROSIS --INFLAMMATION, CHRONIC, SYNOVIAL LN, POPLITEAL (PP) ................. NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 1 0 1 1 1 0 0 NASAL TURBINATE (NT) --B-OSTEOMA NUMBER EXAMINED: NOT REMARKABLE: 1 0 1 0 0 0 3748 Covance 6329-212 TABLE 37 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 10 10 10 10 ** TOP OF LIST ** LIVER (LI) ........................ ................... NUMBER EXAMINED: 10 10 10 10 --CYST -> 10 10 10 10 TL> 10 10 10 10 --CYST, BILIARY -> 10 10 10 10 TL> 10 10 10 10 MN> o oooo o oooo o oooo o oooo o ro o o o o oooo o oooo o oooo --DEGENERATION, CYSTIC -> 10 9 10 10 3> 0 1 0 0 TL> 10 10 10 10 MN> --FIBROSIS -> 10 10 10 10 TL> 10 10 10 10 MN> --FIBROSIS, CAPSULAR -> 10 10 10 10 TL> 10 10 10 10 MN> --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -> 10 10 10 10 TL> 10 10 10 10 MN> 3749 Covance 6329-212 TABLE 37 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 10 10 10 10 -- ------------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ ................... NUMBER EXAMINED: 10 10 10 10 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 10 10 10 10 TL> 10 10 10 10 MN> oo oo oo oo oo oo oo oo --HEMATOPOIESIS, EXTRAMEDULLARY -> 10 10 10 10 TL> 10 10 10 10 MN> --HEMORRHAGE -> 8 1> 1 2> 1 TL> 10 MN> 10 9 00 01 10 10 0.2 10 0 0 10 oo oo oo oo o oo o oo ro o o o oo --HYPERPLASIA, BILE DUCT -> 10 10 10 9 1> 0 0 0 1 TL> 10 10 10 10 MN> 0.1 --INFILTRATE, MAST CELLS -> 10 10 10 10 TL> 10 10 10 10 MN> 3750 Covance 6329-212 TABLE 37 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 10 10 10 10 ** FROM PREVIOUS PAGE ** LIVER (LI) ...................................... ..... NUMBER EXAMINED: 10 10 10 10 --INFLAMMATION, CHRONIC -> 9 8 10 10 1> 1 2 0 0 TL> 10 10 10 10 MN> 0.1 0.2 oo oo oo oo o o --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 9 10 10 10 4> 1 0 0 0 TL> 10 10 10 10 MN> 0.4 --NECROSIS, HEPATOCELLULAR -> 9 2> 0 4> 1 TL> 10 MN> 0.4 10 9 01 00 10 10 0.2 10 0 0 10 ooo ooo oo oo ooo ooo oo oo --PIGMENT -> 10 10 10 10 TL> 10 10 10 10 MN> --TELANGIECTASIS -> 10 10 10 10 TL> 10 10 10 10 MN> 3751 Covance 6329-212 TABLE 37 Incidence of Severity of Selected Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 10 10 10 10 ** FROM PREVIOUS PAGE ** -=- -=- -=- -=- LIVER (LI) ......................... .................. NUMBER EXAMINED: 10 10 10 10 --THROMBUS -> 10 10 10 10 TL> 10 10 10 10 MN> oo oo oo oo o ro oo oo oo --VACUOLATION, HEPATOCELLULAR -> 10 8 10 10 1> 0 1 0 0 2> 0 1 0 0 TL> 10 10 10 10 MN> --B-ADENOMA, HEPATOCELLULAR -> 10 9 10 10 +> 0 1 0 0 TL> 10 10 10 10 --B-HEMANGIOMA -> 10 10 10 10 TL> 10 10 10 10 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 10 10 10 10 TL> 10 10 10 10 3752 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 -- ----------------------------------------------- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ............... ............................. NUMBER EXAMINED: 11 20 13 15 --CYST -> 11 20 13 15 TL> 11 20 13 15 --CYST, BILIARY -> 11 20 12 15 3> 0 0 1 0 TL> 11 20 13 15 MN> 0.0 0.2 0.0 oo oo o o o o o oo o oo --DEGENERATION, CYSTIC -> 9 16 12 14 1> 1 0 0 0 2> 0 3 0 0 3> 1 1 1 1 TL> 11 20 13 15 MN> 0.4 0.5 0.2 0.2 --FIBROSIS -> 11 5> 0 TL> 11 MN> 20 12 01 20 13 0.4 15 0 15 --FIBROSIS, CAPSULAR -> 11 19 13 15 2> 0 1 0 0 TL> 11 20 13 15 MN> 0.1 3753 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC .... NUMBER EXAMINED: 11 20 13 -> 11 19 12 2> 0 0 1 3> 0 1 0 TL> 11 20 13 MN> 0.2 0.2 15 13 1 1 15 ro o o o oo oo oo CO o oo oo oo --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 10 1> 0 2> 0 3> 0 4> 1 TL> 11 MN> 0.4 14 10 10 001 2 01 431 002 20 13 15 0.7 0.9 --HEMATOPOIESIS, EXTRAMEDULLARY -> 11 20 13 15 TL> 11 20 13 15 MN> --HEMORRHAGE -> 10 19 13 15 2> 1 0 0 0 3> 0 1 0 0 TL> 11 20 13 15 MN> 0.2 0.2 3754 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 -- ------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ...................................... ..... NUMBER EXAMINED: 11 20 13 15 --HYPERPLASIA, BILE DUCT -> 7 15 11 13 1> 4 5 1 2 2> 0 0 1 0 TL> 11 20 13 15 MN> 0.4 0.2 0.1 o o o o ro o oo o o --INFILTRATE, MAST CELLS -> 11 20 13 15 TL> 11 20 13 15 MN> --INFLAMMATION, CHRONIC -> 5 10 9 1> 6 10 3 2> 0 0 1 TL> 11 20 13 MN> 0.5 0.5 0.4 11 4 0 15 ro o oo o o o o o o --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 11 20 13 15 TL> 11 20 13 15 MN> --NECROSIS, HEPATOCELLULAR -> 11 5> 0 TL> 11 MN> o o o o o o 20 12 01 20 13 0.4 15 0 15 3755 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 ** FROM PREVIOUS PAGE ** LIVER (LI) ...................... ..................... NUMBER EXAMINED: 11 20 13 15 --PIGMENT -> 11 20 13 15 TL> 11 20 13 15 MN> o ro oo oo oo ooo ooo ooo ooo --TELANGIECTASIS -> 11 1> 0 TL> 11 MN> 20 10 13 032 20 13 15 0.2 0.1 --THROMBUS -> 11 20 13 14 4> 0 0 0 1 TL> 11 20 13 15 MN> --VACUOLATION, HEPATOCELLULAR -> 11 15 1> 0 1 2> 0 4 TL> 11 20 MN> 0.5 11 14 01 20 13 15 0.1 ro o o o --B-ADENOMA, HEPATOCELLULAR -> 10 19 12 15 +> 1 1 1 0 TL> 11 20 13 15 --B-HEMANGIOMA -> 11 20 13 15 TL> 11 20 13 15 3756 Covance 6329-212 TABLE 38 Incidence of Severity of Selected Microscopic Observations Terminal Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=T;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 11 20 13 15 ** FROM PREVIOUS PAGE ** -=- -=- -=- -=- LIVER (LI) ......................... .................. NUMBER EXAMINED: 11 20 13 15 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) -> 11 18 13 15 +> 0 2 0 0 TL> 11 20 13 15 ** END OF LIST ** 3757 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 -- ----------------------------------------------- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ............... ............................. NUMBER EXAMINED: 39 30 37 35 --CYST -> 37 30 37 35 P> 2 0 0 0 TL> 39 30 37 35 --CYST, BILIARY -> 39 3> 0 TL> 39 MN> 30 36 01 30 37 0.1 35 0 35 o ooo o ooo ooo ooo o oo o oo o oo o oo --DEGENERATION, CYSTIC -> 36 26 37 34 1> 1 0 0 1 3> 2 2 0 0 4> 0 2 0 0 TL> 39 30 37 35 MN> 0.2 0.5 --FIBROSIS -> 39 30 37 35 TL> 39 30 37 35 MN> --FIBROSIS, CAPSULAR -> 39 30 37 35 TL> 39 30 37 35 MN> 3758 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC .... NUMBER EXAMINED: 39 30 37 35 -> 39 30 37 34 3> 0 0 0 1 TL> 39 30 37 35 MN> 0.0 0.0 0.0 0.1 --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 36 27 37 33 2> 1 0 0 1 3> 2 3 0 1 TL> 39 30 37 35 MN> 0.2 0.3 0.0 0.1 --HEMATOPOIESIS, EXTRAMEDULLARY -> 37 1> 2 2> 0 TL> 39 MN> 0.1 29 1 0 30 0.0 o o 36 34 10 01 37 35 0.1 --HEMORRHAGE -> 37 29 36 33 1> 0 0 0 2 2> 1 1 1 0 3> 1 0 0 0 TL> 39 30 37 35 MN> 0.1 0.1 0.1 0.1 3759 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 ** FROM PREVIOUS PAGE ** LIVER (LI) ...................................... ..... NUMBER EXAMINED: 39 30 37 35 --HYPERPLASIA, BILE DUCT -> 36 27 34 31 1> 2 3 2 4 3> 1 0 1 0 TL> 39 30 37 35 MN> 0.1 0.1 0.1 0.1 --INFILTRATE, MAST CELLS -> 39 29 37 35 2> 0 1 0 0 TL> 39 30 37 35 MN> 0.0 0.1 0.0 0.0 --INFLAMMATION, CHRONIC -> 29 27 33 33 1> 10 2 4 2 3> 0 1 0 0 TL> 39 30 37 35 MN> 0.3 0.2 0.1 0.1 --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 38 29 37 35 4> 1 1 0 0 TL> 39 30 37 35 MN> 0.1 0.1 o o o o 3760 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 ** FROM PREVIOUS PAGE ** LIVER (LI) ................... ........................ NUMBER EXAMINED: 39 30 37 35 --NECROSIS, HEPATOCELLULAR -> 38 27 36 33 1> 0 0 1 0 2> 0 1 0 1 3> 1 2 0 1 TL> 39 30 37 35 MN> 0.1 0.1 o o oo oo ro o oo o o --PIGMENT -> 39 30 36 35 1> 0 0 1 0 TL> 39 30 37 35 MN> --TELANGIECTASIS -> 39 1> 0 5> 0 TL> 39 MN> 30 35 01 01 30 37 0.2 35 0 0 35 oo oo o o oo oo oo oo --THROMBUS -> 39 30 37 35 TL> 39 30 37 35 MN> 3761 Covance 6329-212 TABLE 39 Incidence of Severity of Selected Microscopic Observations Unscheduled Deaths 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=D,M;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 39 30 37 35 ** FROM PREVIOUS PAGE ** -=- -=- -=- -=- LIVER (LI) ......................... .................. NUMBER EXAMINED: 39 30 37 35 --VACUOLATION, HEPATOCELLULAR -> 25 27 25 28 1> 2 0 2 2 2> 3 1 5 2 3> 6 1 3 0 4> 1 1 1 3 5> 2 0 1 0 TL> 39 30 37 35 MN> 1.0 CO o ro o 0.5 --B-ADENOMA, HEPATOCELLULAR -> 35 28 36 34 +> 4 2 1 1 TL> 39 30 37 35 --B-HEMANGIOMA -> 39 30 37 35 TL> 39 30 37 35 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 38 28 37 34 +> 1 2 0 1 TL> 39 30 37 35 3762 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 -------------------------------- ------------------------------------------- -=- -=- -=- -=- ** TOP OF LIST ** LIVER (LI) ................... ........................ NUMBER EXAMINED: 60 60 60 60 --CYST -> 58 60 60 60 P> 2 0 0 0 TL> 60 60 60 60 --CYST, BILIARY -> 60 60 58 60 3> 0 0 2 0 TL> 60 60 60 60 MN> 0.0 0.1 0.0 oo oo o o oo oo o oo o oo --DEGENERATION, CYSTIC -> 55 51 59 58 1> 2 0 0 1 2> 0 3 0 0 3> 3 4 1 1 4> 0 2 0 0 TL> 60 60 60 60 MN> 0.2 0.4 0.1 0.1 --FIBROSIS -> 60 5> 0 TL> 60 MN> 60 59 01 60 60 0.1 60 0 60 --FIBROSIS, CAPSULAR -> 60 59 60 60 2> 0 1 0 0 TL> 60 60 60 60 MN> 3763 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 ** FROM PREVIOUS PAGE ** LIVER (LI) ........................ --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC .... NUMBER EXAMINED: 60 60 60 60 -> 60 59 59 57 2> 0 0 1 1 3> 0 1 0 2 TL> 60 60 60 60 MN> 0.1 0.0 0.1 oo oo --FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -> 56 51 57 1> 0 0 0 2> 1 2 0 3> 2 7 3 4> 1 0 0 TL> 60 60 60 MN> 0.2 0.4 0.2 53 1 2 2 2 60 ro o oo --HEMATOPOIESIS, EXTRAMEDULLARY o o -> 58 59 59 59 1> 2 1 1 0 2> 0 0 0 1 TL> 60 60 60 60 MN> o o --HEMORRHAGE -> 55 58 58 1> 1 0 0 2> 3 1 2 3> 1 1 0 TL> 60 60 60 MN> 0.2 0.1 0.1 o o 58 2 0 0 60 3764 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 -- ------------------------ -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ...................................... ..... NUMBER EXAMINED: 60 60 60 60 --HYPERPLASIA, BILE DUCT -> 53 52 55 53 1> 6 8 3 7 2> 0 0 1 0 3> 1 0 1 0 TL> 60 60 60 60 MN> 0.2 0.1 0.1 0.1 --INFILTRATE, MAST CELLS -> 60 59 60 60 2> 0 1 0 0 TL> 60 60 60 60 MN> 0.0 0.0 0.0 0.0 --INFLAMMATION, CHRONIC -> 43 1> 17 2> 0 3> 0 TL> 60 MN> 0.3 45 52 54 14 7 6 010 100 60 60 60 0.2 0.1 o o o o ro o --LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS) -> 58 59 60 60 4> 2 1 0 0 TL> 60 60 60 60 MN> 0.1 0.1 3765 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 -------------------------------- ------------------------------------------- -=- -=- -=- -=- ** FROM PREVIOUS PAGE ** LIVER (LI) ................... ........................ NUMBER EXAMINED: 60 60 60 60 --NECROSIS, HEPATOCELLULAR -> 58 57 57 58 1> 0 0 1 0 2> 0 1 1 1 3> 1 2 0 1 4> 1 0 0 0 5> 0 0 1 0 TL> 60 60 60 60 MN> 0.1 0.1 0.1 0.1 --PIGMENT -> 60 60 59 60 1> 0 0 1 0 TL> 60 60 60 60 MN> oo oo oo oo o oo o oo o oo o oo --TELANGIECTASIS -> 60 1> 0 5> 0 TL> 60 MN> 60 55 04 01 60 60 0.2 58 2 0 60 --THROMBUS -> 60 60 60 59 4> 0 0 0 1 TL> 60 60 60 60 MN> 0.1 3766 Covance 6329-212 TABLE 40 Incidence of Severity of Selected Microscopic Observations All Animals 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=ALL;FIND=ALL;SUBSET=T -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9- ORGAN/TISSUE EXAMINED NUMBER: 70 70 70 70 ** FROM PREVIOUS PAGE ** -=- -=- -=- -=- LIVER (LI) ......................... .................. NUMBER EXAMINED: 60 60 60 60 --VACUOLATION, HEPATOCELLULAR -> 46 50 46 52 1> 2 2 2 3 2> 3 6 7 2 3> 6 1 3 0 4> 1 1 1 3 5> 2 0 1 0 TL> 60 60 60 60 MN> 0.7 0.4 0.6 ro o --B-ADENOMA, HEPATOCELLULAR -> 55 56 58 59 +> 5 4 2 1 TL> 60 60 60 60 --B-HEMANGIOMA -> 60 60 60 60 TL> 60 60 60 60 --X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 59 56 60 59 +> 1 4 0 1 TL> 60 60 60 60 3767 APPENDIX 1 P ro to c o l D e v iatio n s Covance 6329-212 Covance 6329-228 3M T-6316.1 3768 Protocol Deviations Protocol. T e s t M a t e r i a l . P u r i t y . 9 8 . 1 % N - E t F O S E ( w / w ) . Covance 6329-212 Covance 6329-228 3M T-6316.1 Actual Procedure. T h e p u r i t y o f t h e t e s t m a t e r i a l , a s d e t e r m i n e d b y C e n t r e A n a l y t i c a l L a b o ra to rie s, In c. o n N o v e m b e r 2 6 , 2 0 0 0 , w a s 9 7 .4 % . Protocol. D o s i n g P r o c e d u r e s . M e t h o d o f A d m i n i s t r a t i o n . A n i m a l s w i l l r e c e i v e t e s t d i e t fo r at least 104 w eek s. Actual Procedure. A n i m a l N o . C 9 2 3 9 2 , G r o u p 8 f e m a l e , w a s f o u n d w i t h o u t f e e d o n S ep tem b er 21, 1998. Protocol. O b s e r v a t i o n o f A n i m a l s . C l i n i c a l O b s e r v a t i o n s . A n i m a l s w i l l b e o b s e r v e e d tw ic e d a ily (a .m . a n d p .m .). P e r s ta n d a rd o p e ra tin g p ro c e d u re , p .m . c h e c k s m u st b e d o n e a t le a s t 6 h o u rs a fte r th e a.m . c h e c k a n d n o t b e fo re 1 2 :0 0 p .m . Actual Procedure. T h e p . m . r o o m c h e c k w a s d o n e a t 1 1 : 5 2 o n J u n e 3 1 , 1 9 9 8 . T h e p . m . ro o m c h ec k w a s d o n e 1 7 m in u tes early o n Ja n u a ry 2 5 , 2 0 0 0 . Protocol. O b s e r v a t i o n o f A n i m a l s . B o d y W e i g h t s . B o d y w e i g h t s w i l l b e t a k e n w e e k l y fo r W ee k s 1 th ro u g h 17. Actual Procedure. T h e b o d y w e i g h t t a k e n o n M a y 2 5 , 1 9 9 8 , f o r A n i m a l N o . C 9 2 4 4 7 . G ro u p 9 fe m a le , w a s e x c lu d e d d u e to a w e ig h in g erro r. T h e b o d y w e ig h t ta k e n fo r A n im a l N o . C 9 2 3 4 5 , G ro u p 8 fe m a le , w a s e x c lu d e d d u e to a w e ig h in g erro r. Protocol. O b s e r v a t i o n o f A n i m a l s . F o o d C o n s u m p t i o n . A n i m a l s w i l l h a v e f o o d c o n su m p tio n re c o rd e d w e ek ly fo r W e e k s 1 th ro u g h 16 an d o n ce ev ery 4 w e e k s th ere afte r. 3769 Protocol Deviations (Continued) Covance 6329-212 Covance 6329-228 3M T-6316.1 Actual Procedure. F o o d c o n s u m p t i o n v a l u e s w e r e e x c l u d e d f o r W e e k 1 4 f o r a n i m a l s w h e re e m p ty fe ed e r w e ig h ts w e re n o t d o c u m e n ted . D o c u m e n ta tio n o f sp illed fe ed m ay h a v e n o t b e e n re c o rd e d d u rin g W e e k 7 6 , th e re fo re fo o d c o n su m p tio n d a ta m a y b e in erro r. Protocol. P o s t m o r t e m P r o c e d u r e s . P a l m i t o y l - C o A O x i d a s e T i s s u e C o l l e c t i o n a n d A n a ly se s. A t th e W e e k 14 sa c rifice , a sa m p le o f th e rig h t late ra l lo b e w ill b e fla sh -fro z e n in liq u id n itro g e n . Actual Procedure. F l a s h - f r e e z i n g o f t h e l i v e r a t W e e k 1 4 w a s n o t d o c u m e n t e d . Protocol. P o s t m o r t e m P r o c e d u r e s . O r g a n W e i g h t s . L i v e r w i l l b e w e i g h e d a t t h e W e e k 53 in te rim sacrifice. Actual Procedure. T h e l i v e r w a s n o t w e i g h e d f o r A n i m a l N o . C 9 2 3 2 6 , G r o u p 9 m a l e . Protocol. P o s t m o r t e m P r o c e d u r e s . T i s s u e P r e s e r v a t i o n . A s e l e c t e d l i s t o f t i s s u e s w i l l b e c o lle c te d fro m e a c h a n im a l a n d sto re d in 1 0 % n e u tra l-b u ffe re d fo rm alin . Actual Procedure. S e l e c t e d t i s s u e s t o b e h e l d a n d n o t e x a m i n e d m i c r o s c o p i c a l l y f o r a n im a ls in G ro u p 9 w e re trim m ed . T h ese tissu e s w e re re tu rn e d to w e t tissu e an d n o t p ro c e ss e d to slid es. Protocol. P o s t m o r t e m P r o c e d u r e s . H i s t o p a t h o l o g y . S e l e c t e d t i s s u e s f r o m e a c h a n i m a l in G ro u p s 8 a n d 9 w ill b e e x a m in e d m ic ro sc o p ic a lly . 3770 Protocol Deviations (Continued) Covance 6329-212 Covance 6329-228 3M T-6316.1 Actual Procedure. S o m e t i s s u e s , r e q u i r e d b y p r o t o c o l , w e r e n o t a v a i l a b l e f o r h isto p a th o lo g u c e x a m in a tio n . M iss in g tis su e s a re liste d w ith a p p ro p ria te c o m m e n ts in th e p a th o lo g y d a ta sh e e ts fo r in d iv id u a l a n im a ls. S u m m a ry ta b le s d o n o t in c lu d e th e m as h a v in g b e e n ex am in ed . T h e se d e v ia tio n s a re n o t e x p e c te d to h a v e a ffe c te d th e re su lts o f th e study. 3771 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta Covance 6329-212 FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-228 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e X o f X II Page 3772 of 4873 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME X OF XII CONTENTS Page A P P E N D I X 2 ...................................................................................................................................................................................... 3 7 7 4 I n d i v i d u a l A n i m a l F a t e D a t a .......................................................................................................................................... 3 7 7 5 I n d i v i d u a l C l i n i c a l O b s e r v a t i o n s ................................................................................................................................3 7 8 4 A P P E N D I X 3 ......................................................................................................................................................................................4 0 1 7 I n d i v i d u a l B o d y W e i g h t D a t a ( g ) .............................................................................................................................. 4 0 1 8 A P P E N D I X 4 ...................................................................................................................................................................................... 4 0 5 1 I n d i v i d u a l F o o d C o n s u m p t i o n D a t a ( g ) ...............................................................................................................4 0 5 2 I n d i v i d u a l T e s t M a t e r i a l C o n s u m p t i o n D a t a ( m g / k g / d a y ) ................................................................. 4 0 8 5 A P P E N D I X 5 ......................................................................................................................................................................................4 1 0 5 I n d i v i d u a l C l i n i c a l H e m a t o l o g y D a t a .................................................................................................................... 4 1 0 6 I n d i v i d u a l C l i n i c a l C h e m i s t r y D a t a ......................................................................................................................... 4 1 2 4 I n d i v i d u a l C l i n i c a l U r i n a l y s i s D a t a ......................................................................................................................... 4 1 4 0 I n d i v i d u a l U r i n e C h e m i s t r y D a t a .............................................................................................................................. 4 1 5 8 3773 APPENDIX 2 In d iv id u a l A n im al F a te D a ta In d iv id u a l C lin ic al O b se rv a tio n s Covance 6329-212 Covance 6329-228 3M T-6316.1 3774 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 ANIMAL DOSE NUMBER GROUP C92191 C92192 C92193 C92194 C92195 C92196 C92197 C92198 C92199 C92200 C92201 C92202 C92203 C92204 C92205 C92206 C92207 C92208 C92209 C92210 C92211 C92212 C92213 C92214 C92215 C92216 C92217 C92218 C92219 C92220 C92221 C92222 C92223 C92224 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 03/27/00 04/28/98 04/04/00 03/11/00 04/07/99 04/28/98 04/04/00 06/19/98 04/07/99 09/21/99 01/27/00 11/25/99 04/04/00 10/27/99 04/07/99 09/14/99 04/28/98 04/04/00 04/04/00 02/23/00 04/07/99 03/27/00 03/27/99 11/15/99 09/14/99 04/28/98 10/04/99 04/04/00 12/03/99 09/25/99 01/10/00 04/04/00 04/07/99 03/21/00 722 23 730 706 367 23 730 75 367 534 662 599 730 570 367 527 23 730 730 689 367 722 356 589 527 23 547 730 607 538 645 730 367 716 WEEK OF STUDY 104 4 105 101 53 4 105 11 53 77 95 86 105 82 53 76 4 105 105 99 53 104 51 85 76 4 79 105 87 77 93 105 53 103 3775 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 ANIMAL DOSE NUMBER GROUP C92225 C92226 C92227 C92228 C92229 C92230 C92231 C92232 C92233 C92234 C92235 C92236 C92237 C92238 C92239 C92240 C92241 C92242 C92243 C92244 C92245 C92246 C92247 C92248 C92249 C92250 C92251 C92252 C92253 C92254 C92255 C92256 C92257 C92258 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE DATE OF DAY OF DEATH STUDY 08/12/99 12/27/99 03/17/00 06/25/99 11/26/99 04/07/99 04/04/00 02/28/00 04/04/00 11/15/99 02/11/00 12/20/99 03/11/00 04/07/99 04/04/00 11/04/99 01/10/00 07/14/99 08/03/99 07/02/99 07/25/99 04/07/99 04/07/99 07/26/99 08/17/99 01/02/00 11/08/99 04/04/00 04/07/99 11/29/99 07/07/98 07/07/98 07/07/98 07/07/98 494 631 712 446 600 367 730 694 730 589 677 624 706 367 730 578 645 465 485 453 476 367 367 477 499 637 582 730 367 603 93 93 93 93 WEEK OF STUDY 71 91 102 64 86 53 105 100 105 85 97 90 101 53 105 83 93 67 70 65 68 53 53 69 72 91 84 105 53 87 14 14 14 14 3776 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 ANIMAL DOSE NUMBER GROUP C92259 C92260 C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 C92292 8 8 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/28/98 07/07/98 04/04/00 03/27/00 04/04/00 04/28/98 10/16/99 04/04/00 04/07/99 02/28/00 08/24/99 04/04/00 12/21/99 11/16/99 01/24/99 04/07/99 03/27/00 08/28/99 05/25/99 04/04/00 12/13/99 04/28/98 04/04/00 04/04/00 04/04/00 04/28/98 09/14/99 04/07/99 04/07/99 04/07/99 04/04/00 06/17/99 02/07/00 07/12/99 23 93 730 722 730 23 559 730 367 694 506 730 625 590 294 367 722 510 415 730 617 23 730 730 730 23 527 367 367 367 730 438 673 463 WEEK OF STUDY 4 14 105 104 105 4 80 105 53 100 73 105 90 85 42 53 104 73 60 105 89 4 105 105 105 4 76 53 53 53 105 63 97 67 3777 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 ANIMAL DOSE NUMBER GROUP C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 C92323 C92324 C92325 C92326 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 SEX MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE MALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE DATE OF DAY OF DEATH STUDY 01/18/99 02/07/00 04/04/00 09/12/99 04/04/00 07/19/99 04/04/00 04/28/98 12/01/99 02/02/00 04/07/99 01/31/00 06/17/99 03/27/00 03/12/00 04/04/00 04/04/00 08/25/99 04/04/00 06/28/99 02/08/00 01/14/00 04/07/99 03/07/00 04/07/99 04/04/00 04/04/00 04/07/99 04/04/00 04/04/00 04/04/00 04/07/99 07/07/98 07/07/98 288 673 730 525 730 470 730 23 605 668 367 666 438 722 707 730 730 507 730 449 674 649 367 702 367 730 730 367 730 730 730 367 93 93 WEEK OF STUDY 42 97 105 75 105 68 105 4 87 96 53 96 63 104 101 105 105 73 105 65 97 93 53 101 53 105 105 53 105 105 105 53 14 14 3778 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 ANIMAL DOSE NUMBER GROUP SEX C92327 C92328 C92329 C92330 C92331 C92332 C92333 C92334 C92335 C92336 C92337 C92338 C92339 C92340 C92341 C92342 C92343 C92344 C92345 C92346 C92347 C92348 C92349 C92350 C92351 C92352 C92353 C92354 C92355 C92356 C92357 C92358 C92359 C92360 9 MALE 9 MALE 9 MALE 9 MALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE DATE OF DAY OF DEATH STUDY 07/07/98 07/07/98 04/28/98 07/07/98 01/06/00 04/28/98 04/04/00 04/04/00 05/29/98 10/27/99 10/26/99 08/02/99 01/17/00 10/05/99 04/07/99 03/15/99 05/10/99 03/27/00 10/28/98 06/14/99 03/01/99 04/04/00 09/28/99 01/21/00 04/04/00 04/28/98 02/01/00 04/04/00 04/28/98 04/07/99 04/07/99 04/04/00 01/13/00 04/04/00 93 93 23 93 641 23 730 730 54 570 569 484 652 548 367 344 400 722 206 435 330 730 541 656 730 23 667 730 23 367 367 730 648 730 WEEK OF STUDY 14 14 4 14 92 4 105 105 8 82 82 70 94 79 53 50 58 104 30 63 48 105 78 94 105 4 96 105 4 53 53 105 93 105 3779 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 ANIMAL DOSE NUMBER GROUP SEX C92361 8 FEMALE C92362 8 FEMALE C92363 8 FEMALE C92364 8 FEMALE C92365 8 FEMALE C92366 8 FEMALE C92367 8 FEMALE C92368 8 FEMALE C92369 8 FEMALE C92370 8 FEMALE C92371 8 FEMALE C92372 8 FEMALE C92373 8 FEMALE C92374 8 FEMALE C92375 8 FEMALE C92376 8 FEMALE C92377 8 FEMALE C92378 8 FEMALE C92379 8 FEMALE C92380 8 FEMALE C92381 8 FEMALE C92382 8 FEMALE C92383 8 FEMALE C92384 8 FEMALE C92385 8 FEMALE C92386 8 FEMALE C92387 8 FEMALE C92388 8 FEMALE C92389 8 FEMALE C92390 8 FEMALE C92391 8 FEMALE C92392 8 FEMALE C92393 8 FEMALE C92394 8 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 1 SCHEDULED FIRST INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 03/07/00 04/04/00 11/01/99 06/17/99 04/07/99 04/28/98 04/04/00 03/17/00 04/04/00 03/02/00 12/15/99 04/07/99 04/28/98 04/04/00 12/01/99 04/07/99 05/03/99 08/26/99 08/16/99 10/18/99 07/29/99 05/03/99 04/04/00 12/10/99 01/31/00 04/04/00 08/31/99 04/07/99 04/07/99 04/07/99 01/12/00 06/21/99 01/18/00 11/15/99 702 730 575 438 367 23 730 712 730 697 619 367 23 730 605 367 393 508 498 561 480 393 730 614 666 730 513 367 367 367 647 442 653 589 WEEK OF STUDY 101 105 83 63 53 4 105 102 105 100 89 53 4 105 87 53 57 73 72 81 69 57 105 88 96 105 74 53 53 53 93 64 94 85 3780 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 ANIMAL DOSE NUMBER GROUP SEX C92395 C92396 C92397 C92398 C92399 C92400 C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 8 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE 2 SCHEDULED SECOND INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 04/07/99 07/07/98 07/07/98 07/07/98 07/07/98 07/07/98 01/06/00 04/28/98 03/27/99 10/23/99 02/20/00 04/07/99 04/07/99 03/27/00 02/25/00 04/04/00 04/07/99 04/04/00 03/15/00 06/14/99 12/15/99 04/04/00 01/18/00 04/07/99 04/04/00 08/23/99 04/04/00 04/07/99 04/04/00 04/07/99 02/28/00 04/04/00 06/14/99 06/07/99 367 93 93 93 93 93 641 23 356 566 686 367 367 722 691 730 367 730 710 435 619 730 653 367 730 505 730 367 730 367 694 730 435 428 WEEK OF STUDY 53 14 14 14 14 14 92 4 51 81 98 53 53 104 99 105 53 105 102 63 89 105 94 53 105 73 105 53 105 53 100 105 63 62 3781 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 ANIMAL DOSE NUMBER GROUP SEX C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE DEATH CODE TYPE OF DEATH DESCRIPTION OF DEATH D UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 1 SCHEDULED FIRST INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE T SCHEDULED TERMINAL SACRIFICE D UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH 3 SCHEDULED THIRD INTERIM SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH T SCHEDULED TERMINAL SACRIFICE M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH M UNSCHEDULED UNSCHEDULED DEATH DATE OF DAY OF DEATH STUDY 06/10/99 04/04/00 04/04/00 09/24/99 04/07/99 12/29/99 04/28/98 01/31/00 03/19/99 04/07/99 04/04/00 10/17/99 04/07/99 07/25/99 04/04/00 04/04/00 01/31/00 11/05/99 01/03/00 08/02/99 04/07/99 12/15/99 04/04/00 04/04/00 02/28/00 10/23/99 03/23/00 04/04/00 11/15/99 07/19/99 02/28/00 02/13/00 06/04/99 02/21/00 431 730 730 537 367 633 23 666 348 367 730 560 367 476 730 730 666 579 638 484 367 619 730 730 694 566 718 730 589 470 694 679 425 687 WEEK OF STUDY 62 105 105 77 53 91 4 96 50 53 105 80 53 68 105 105 96 83 92 70 53 89 105 105 100 81 103 105 85 68 100 97 61 99 3782 Covance 6329-212 APPENDIX 2 Individual Animal Fate Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 9 ANIMAL DOSE NUMBER GROUP SEX C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE 9 FEMALE DEATH CODE 1 2 2 2 1 2 2 1 TYPE OF DEATH SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED SCHEDULED DESCRIPTION OF DEATH FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE SECOND INTERIM SACRIFICE SECOND INTERIM SACRIFICE FIRST INTERIM SACRIFICE DATE OF DAY OF DEATH STUDY 04/28/98 07/07/98 07/07/98 07/07/98 04/28/98 07/07/98 07/07/98 04/28/98 23 93 93 93 23 93 93 23 WEEK OF STUDY 4 14 14 14 4 14 14 4 3783 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92191 M 104 APPEARANCE SWOLLEN PAW-HIND-LEFT EYES PALE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 64 C92192 1 4 NORMAL NO REMARKABLE OBSERVATIONS 708 701, 708, 715, 722 1, 8,. 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 442C,. 449C, 456C, 463C, 470C, 477C , 484C, 491C, 498C, 505C , 512C 519C,. 526C, 533C, 540C, 547C, 554C , 561C, 568C, 575C, 582C , 589C 596C, 603C, 610C, 617C, 624C, 631C , 638C, 645C, 652C, 659C , 666C 673C,. 680C, 687C, 694C, 701C, 708C , 715C, 722C 1, 8, 15, 22-23 3784 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92193 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92194 M 101 BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION NO FECES EYES DILATED PUPIL EYES PALE EYES RESPIRATION AUDIBLE LABORED 701, 708, 715,. 722, 729- 730 1, 8,r 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 659, 666, 22, 29, 36 , 43 , 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 673, 680, 687, 694 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 645 , 652 706 706 706 706 706 706 706 3785 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92194 M 101 NORMAL NO REMARKABLE OBSERVATIONS C92195 3 NORMAL NO REMARKABLE OBSERVATIONS 1 C92197 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-LEFT THIN EXCRETION FEW FECES DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 , 15, 22, 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 , 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 , 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 , 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 , 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 , 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 , 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673 , 680, 687, 694, 701 1, 8 , 15, 22, 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 , 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 , 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 , 316, 323, 330, 337, 344 , 351, 358, 365, 367 1, 8 , 15, 22- 23 337, 428, 519, 610, 701, 344, 435, 526, 617, 708, 351 , 358, 442 , 449, 533 , 540, 624 , 631, 715 , 722, 365, 372, 456, 463, 547, 554, 638, 645, 729- 730 379, 470, 561, 652, 386 , 393, 477 , 484, 568 , 575, 659 , 666, 400, 491, 582, 673, 407, 498, 589, 680, 414 , 421 505 , 512 596 , 603 687 , 694 729- 730 582, 708, 715 , 722, 729- 730 498, 505 3786 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER (CONTINUED FROM PREVIOUS PAGE) C92197 T 105 SKIN & PELAGE SORE/SCAB DORSAL GROUP: M8 NORMAL NO REMARKABLE OBSERVATIONS C92198 D C92199 3 11 NORMAL NO REMARKABLE OBSERVATIONS 53 SKIN & PELAGE SORE/SCAB SCROTUM NORMAL NO REMARKABLE OBSERVATIONS C92200 M 77 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701,. 708, 715 722, 729- 730 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 75 337, 344, 351 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 358, 365, 367 526, 533-534 526, 533-534 3787 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92200 M 77 EYES RED DISCHARGE EYE-LEFT EYES SKIN & PELAGE SORE/SCAB TAIL-PROXIMAL NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) NOSE WEEK 68 TAIL-PROXIMAL WEEK 74 M 95 APPEARANCE LIMITED USE LIMBS-HIND EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 519, 526 533-534 498, 505 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365,. 372, 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456, 463 470, 477, 484, 491 512, 519, 526, 533-534 659, 662 662 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 3788 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92201 M 95 NORMAL NO REMARKABLE OBSERVATIONS D 86 APPEARANCE MALOCCLUSION SWOLLEN PAW-HIND-RIGHT THIN DISCHARGE OTHER EXCRETION FEW FECES RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 295, 386, 477, 568, 211, 302, 393, 484, 575, 218, 225, 309,. 316, 400, 407, 491, 498, 582, 589, 232, 323, 414, 505, 596, 239, 330, 421, 512, 603, 246, 337, 428, 519, 610, 253 , 260, 344 , 351, 435 , 442, 526 , 533, 617 , 624, 267, 358, 449, 540, 631, 274, 365, 456, 547, 638, 281 , 288 372 , 379 463 , 470 554 , 561 645 , 652 592, 596, 599 491, 498, 505 589, 592, 596, 599 592C 582, 589, 592,. 596, 599 592 1, 8,, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 512, 519, 526, 533, 540, 547 , 554, 561, 568, 575 3789 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92203 T 105 APPEARANCE OBESE SWOLLEN PAW-HIND-LEFT THIN NORMAL NO REMARKABLE OBSERVATIONS C92204 D 82 NORMAL NO REMARKABLE OBSERVATIONS C92205 3 53 NORMAL NO REMARKABLE OBSERVATIONS 526, 533 540, 547,. 554, 561,. 568, 575, 582, 589, 596, 603, 610 617, 624 631, 638, 645,. 652, 659 693- 694, 01, 708, 715, 722, 729-730 729- 730 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505, 512, 519, 666 , 673, 680, 687 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 570 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 1, 8 , 15 113, 120 204, 211 295, 302 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 3790 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92206 M 76 BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS C92207 1 C92208 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 APPEARANCE MALOCCLUSION EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-RIGHT 526-527 512, 519 526-527 1, 8,r 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43,r 50, 127 , 134 , 141, 148, 218 , 225 , 232, 239, 309 , 316 , 323, 330, 400 , 407 , 414, 421, 491 , 498 , 505 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 1, 8,r 15, 22- 23 148, 155, 162 , 169 , 176, 183, 190, 197 , 204, 211, 218, 225 , 232 239, 246, 253 , 260 , 267, 274, 281, 288 , 295, 302, 309, 316 , 323 708 722, 729-730 3791 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 9 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92208 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92209 T 105 APPEARANCE MALOCCLUSION THIN EYES RED DISCHARGE EYES RESPIRATION AUDIBLE SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8 , 15 , 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85 113, 120 , 127, 134 , 141, 330, 337, 344 , 351, 358 386, 393 , 400, 407 , 414, 421, 428, 435 , 442, 449 477, 484 , 491, 498 , 505, 512, 519, 526 , 533, 540 568, 575 , 582, 589 , 596, 603, 610, 617 , 624, 631 659, 666 , 673, 680 , 687, 694, 701, 715 92, 99, 106 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 645 , 652 71, 78, 85, 92 , 99 , 106, 169, 176 , 183, 190 , 197, 260, 267 , 274, 281 , 288, 351, 358 , 365, 372 , 379, 442, 449 , 456, 463 , 470, 533, 540 , 547, 554 , 561, 624, 631 , 638, 645 , 652, 715, 722 , 729- 730 701, 708 113, 204, 295, 386, 477, 568, 659, 120, 211, 302, 393, 484, 575, 666, 127 , 134, 218 , 225, 309 , 316, 400 , 407, 491 , 498, 582 , 589, 673 , 680, 141 232 323 414 505 596 687 148, 239, 330, 421, 512, 603, 694, 155, 246, 337, 428, 519, 610, 701, 162 253 344 435 526 617 708 64 64 701, 708, 715,, 722, 729-730 1, 8,, 15, 22, 29, 36, 43, 50, 57 3792 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 10 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92210 M 99 APPEARANCE MALOCCLUSION BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES NONFORMED FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE PERINEAL AREA YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 64, 71, 78, 85,r 92, 99, 106, 113, 120, 127, 134, 141, 148, 155 162, 169 , 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246 253, 260 , 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 344, 351 , 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428 435, 442 , 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519 526, 533 , 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624 , 631, 638, 645, 652, 659, 666, 673, 680, 683, 687- 689 688-689 688 688 689 393, 652 683, 687-688 689 687-689 1, 8, 15, 22, 29, 36, 43, 50, 57 3793 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92211 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92212 M 104 APPEARANCE THIN DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES NONFORMED FECES SKIN & PELAGE PALE ENTIRE BODY RED HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 701, 708, 714-715, 718, 722 714-715, 718, 722 714-715 718, 722 204 722 715, 718 722 1, 8, 15 113, 120 211, 218 302, 309 393, 400 484, 491 575, 582 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 498, 505 , 512, 519, 526, 533 , 540, 547, 554, 561 , 568 589, 596 , 603, 610, 617, 624 , 631, 638, 645, 652 , 659 3794 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92212 M 104 NORMAL NO REMARKABLE OBSERVATIONS C92213 M 51 BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C92214 M 85 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION NO FECES DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 666, 673, 680, 687, 694 356 356 351, 356 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225 , 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 589 589 589 589 3795 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 13 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92214 M 85 SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92215 M 76 APPEARANCE MALOCCLUSION SWOLLEN NOSE BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL-LEFT SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 589 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 428, 435, 442, 449, 470, 477, 484, 491, 498, 505, 512, 519 526- 527 470, 477, 484, 491, 498, 505, 512 526-527 526-527 470, 477, 484, 491, 498, 505, 512, 519, 526-527 512, 519, 526-527 3796 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92215 M 76 SKIN & PELAGE BROWN HAIR COAT VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS C92216 1 C92217 M 4 NORMAL NO REMARKABLE OBSERVATIONS 79 SKIN & PELAGE SORE/SCAB PAW-FRONT-LEFT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 28 VENTRAL-ABDOMINAL-RIGHT WEEK 33 WEEK 63 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 512, 519, 526-527 1, 8,, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 456, 463 1, 8,, 15, 22-23 421 141, 442, 449, 456 197 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 148, 155, 162, 169, 176 , 183, 204, 211 218, 253 260, 267, 274, 281, 288, 295, 302, 309 , 316, 323, 330 337, 344 351, 358, 365,. 372, 379, 386, 393, 400 , 407, 414, 428 190 225C, 232C, 239C, 246C 435C, 442C, 449C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C 3797 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 15 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92217 M 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 20 C92218 T 105 APPEARANCE MALOCCLUSION MISSING TEETH THIN SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 512C, 519C, 526C, 533C, 540C, 547C 134 43, 50, 57, 64 , 71 , 78, 85, 92, 99, 106 , 113 , 120, 127,r 134, 14 148, 155 , 162, 169 , 176, 183 , 190 , 197, 204, 211, 218, 225, 232 239, 246 , 253, 260 , 267, 274 , 281 , 288, 295, 302, 309, 316, 323 330, 337 , 344, 351 , 358, 365 , 372 , 379, 386, 393, 400, 407, 414 421, 428 , 435, 442 , 449, 456 , 463 , 470, 477, 484, 491, 498, 505 512, 519 , 526, 533 , 540, 547 , 554 , 561, 568, 575, 582, 589, 596 603, 610 , 617, 624 , 631, 638 , 645 , 652, 659, 666, 673, 680, 687 693- 694, 701, 708, 715, 722, 729- 730 407, 414 , 421, 428 , 435, 442 , 449 , 456, 463, 470, 498, 505 , 512, 519 , 526, 533 , 540 , 547, 554, 561, 589, 596 , 603, 610 , 617, 624 , 631 , 638, 645, 652, 680, 687 , 693- 694, 701, 708, 715, 722, 729-730 693- 694, 701, 708, 715, 722, 729- 730 477, 568, 659, 484, 575, 666, 491 582 673 386, 400 , 442, 449 , 456, 526 , 533 , 540, 547, 554, 561, 568, 575 582, 589 , 596, 603 , 610, 617 , 624 , 631, 638, 645, 652, 659, 666 673, 680 , 687, 693 -694, 701, 708, 715, 722, 729-730 1, 8 , 15 , 22, 29, 36 3798 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 16 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92218 T 105 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 79 C92219 M 87 BEHAVIOR OTHER EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB TAIL-DISTAL NORMAL NO REMARKABLE OBSERVATIONS C92220 D 77 EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 547, 554, 561 607C 607 607 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603 498 449 3799 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 17 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92220 D 77 NORMAL NO REMARKABLE OBSERVATIONS C92221 M 93 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 456, 463, 470 , 477 484, 491, 505, 512 , 519, 526, 533, 538 645 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645 645 645 645 386, 393, 400, 407, 414, 421 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 3800 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 18 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92221 M 93 NORMAL NO REMARKABLE OBSERVATIONS C92222 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) DORSAL WEEK 95 VENTRAL-ABDOMINAL WEEK 75 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 428, 435, 442, 449 , 456, 463, 470, 477 , 484, 491, 498, 505 , 512 519, 526, 533,. 540 , 547, 554, 561, 568 , 575 526, 533, 540 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708, 715, 722, 729-730C 519C 3801 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 19 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92223 3 53 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92224 D MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 40 VENTRAL-ABDOMINAL-RIGHT WEEK 28 APPEARANCE SWOLLEN PAWS-HIND EXCRETION NONFORMED FECES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB DORSAL-THORACIC-LEFT PAW-HIND-RIGHT PAWS-HIND VENTRAL-ABDOMINAL 295, 302, 309, 316 , 323 225, 232 1, 8,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155,. 162, 169, 176, 183, 239, 246 253, 260, 267, 330 , 337, 344, 351,. 358, 365, 367 274C, 281C, 288C 190, 197, 204, 211C, 218C 708, 715-716 498, 505 715-716 624 582, 603, 694, 232, 589, 610, 697, 239, 596 617, 701, 246, 624, 708, 547, 631, 638, 715-716 554, 561, 645, 568, 652, 575 659, 666, 673, 680, 687 3802 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 20 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92224 D 103 NORMAL NO REMARKABLE OBSERVATIONS C92225 M MASS(ES) VENTRAL-ABDOMINAL WEEK 32 WEEK 78 WART LIKE LESION(S) DORSAL WEEK 93 71 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 253, 260 , 267, 274, 281, 288 , 295, 302, 309, 316 , 323 330, 337, 344, 351 , 358, 365, 372, 379 , 386, 393, 400, 407 , 414 421, 428, 435, 442 , 449, 456, 463, 470 , 477, 484, 491, 512 , 519 526, 533 218C, 225C , 232C, 239C, 246C 540, 547, 554, 561 645, 652, 659, 666, 673, 680, 687, 694, 697, 701, 708, 715-716 477, 484, 491, 494 494 494 491, 494 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 3803 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 21 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92225 M 71 NORMAL NO REMARKABLE OBSERVATIONS C92226 M 91 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EYES RED DISCHARGE EYE-RIGHT RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 624, 631 631 631 631 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596, 603, 610, 617 3804 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 22 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92227 M 102 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES NONFORMED FECES SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 74 190 701, 708, 710-712 708, 710-712 711-712 673, 711-712 710 708, 710-712 519, 526, 533,. 540 , 547, 554, 561, 568 , 575 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 659, 666 , 680, 687, 694 512, 519 3805 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 23 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92228 M 64 APPEARANCE HUNCHED PROTRUDING PENIS THIN BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES RESPIRATION LABORED SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS 442-443, 446 414 421, 428, 435, 442-443, 446 443, 446 421, 428 435, 442-443, 446 443, 446 446 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 12 7,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 3806 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 24 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92229 D 86 APPEARANCE MALOCCLUSION EXCRETION FEW FECES NONFORMED FECES SKIN & PELAGE SORE/SCAB DORSAL DORSAL-CERVICAL-RIGHT LUMBAR-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92230 3 WART LIKE LESION(S) DORSAL WEEK 41 53 NORMAL NO REMARKABLE OBSERVATIONS 211, 218, 225 407, 414, 421 512 498, 505, 512, 519 , 526, 533, 540, 547 , 554, 561, 568, 575 , 582 589, 596, 600 463, 470, 477, 484 , 491 183, 190, 197 1, 8,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 204, 232 , 239 246, 253, 260, 267 , 274 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 3807 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 25 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92231 T 105 EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB VENTRAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92232 M 100 APPEARANCE MALOCCLUSION EXCRETION FEW FECES NO FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 694, 701, 708, 715 729-730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 722 456, 463, 470 673, 680, 687 690, 694 687, 690, 694 690, 694 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 3808 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 26 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92232 M 100 NORMAL NO REMARKABLE OBSERVATIONS T APPEARANCE THIN EXCRETION FEW FECES NO FECES NONFORMED FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 295, 386, 498, 589, 211, 302, 393, 505, 596, 218, 309, 400, 512, 603, 225, 316, 407, 519, 610, 232, 323, 414, 526, 617, 239, 330, 421, 533, 624, 246, 337, 428, 540, 631, 253, 344, 435, 547, 638, 260, 351, 442, 554, 645, 267, 358, 449, 561, 652, 274, 365, 477, 568, 659, 281, 372, 484, 575, 666 288 379 491 582 701, 708, 715, 722, 729- 730 701, 708, 715, 722 106 134 701, 708 715, 722, 729-730 701, 722 729-730 1, 8, 15 120, 127 218, 225 309, 316 400, 407 491, 498 582, 589 673, 680 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113 141,. 148, 155, 162, 169, 176 , 183, 190, 197, 204 , 211 232, 239, 246, 253, 260, 267 , 274, 281, 288, 295 , 302 323, 330, 337, 344, 351, 358 , 365, 372, 379, 386 , 393 414, 421, 428, 435, 442, 449 , 456, 463, 470, 477 , 484 505,. 512, 519, 526, 533, 540 , 547, 554, 561, 568 , 575 596, 603, 610, 617, 624, 631 , 638, 645, 652, 659 , 666 687,. 694 3809 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 27 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92234 M 85 APPEARANCE OBESE THIN EXCRETION FEW FECES NO FECES EYES SQUINTED PERI-ORBITAL HOUSING,FOOD,WATER & MAINT. ANIMAL PUT IN PLASTIC CAGE SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 547, 554, 561, 568, 575, 580, 582, 587 589 580, 582, 587 589 580, 582 587, 589 587 575, 580 582, 587, 589 575, 580 582, 587, 589 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505, 512, 519, 526 , 533, 540 3810 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 28 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92235 M 97 APPEARANCE MALOCCLUSION SWOLLEN NOSE RESPIRATION AUDIBLE LABORED SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED SKIN TAIL NORMAL NO REMARKABLE OBSERVATIONS 281, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 505, 512, 519, 526, 533, 540, 568, 575, 582, 589, 659, 666, 673 677 677 677 677 677 323 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 288 , 295 302, 309, 316, 330, 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 547, 554, 561, 603, 610 , 617, 624, 631, 638 , 645 652 3811 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 29 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92236 D 90 APPEARANCE THIN EXCRETION FEW FECES EYES PALE EYES RESPIRATION IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS C92237 M 101 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL 622, 624 622, 624 622, 624 622, 624 1, 8,r 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 701, 706 701, 706 706 3812 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 30 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92237 M 101 DISCHARGE NASAL RED IN COLOR EXCRETION NO FECES NONFORMED FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL RESPIRATION LABORED SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 701, 706 706 624 701, 706 701, 706 706 512, 519, 52 6, 533 , 540, 547, 554, 561 , 568, 575, 582, 589 , 596 603, 610, 617, 624 , 631, 638, 645, 652 , 659, 666, 673, 680 , 687 694, 701, 706 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505 3813 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 31 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92238 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92239 T 105 APPEARANCE HEAD TILT RIGHT THIN EXCRETION FEW FECES NONFORMED FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 729-730 729-730 701, 708, 715, 722 134 729-730 701, 708, 715, 722 , 729- 730 729- 730 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 141 , 148, 155, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491, 498, 505 , 512, 519, 526, 533 , 540, 547, 554, 561 , 568 3814 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 32 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92239 T 105 NORMAL NO REMARKABLE OBSERVATIONS D 83 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92241 M WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 64 93 APPEARANCE HEAD TILT LEFT EXCRETION FEW FECES NONFORMED FECES EYES RED DISCHARGE EYE-LEFT DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673, 680, 687, 694 533, 540, 547,. 554, 561, 568, 575, 578 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 498, 505, 512, 519 , 526 442, 449, 456, 463, 470, 477, 484, 491 644-645 644-645 498, 505 644-645 3815 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 33 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92241 M 93 SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92242 M 67 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION NO FECES EYES SQUINTED PERI-ORBITAL PERI-ORBITAL-LEFT RESPIRATION IRREGULAR DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 644-645 1, 8,r 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 582, 589, 22, 29, 36, 43,r 50, 127, 134 , 141, 148, 218, 225 , 232, 239, 309, 316 , 323, 330, 400, 407 , 414, 421, 491, 512 , 519, 526, 596, 603 , 610, 617, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 533, 540 , 547, 554, 624, 631 , 638 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 561, 568 , 575 465 465 465 465 465 465 465 3816 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 34 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92242 M 67 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C92243 M 70 APPEARANCE HUNCHED BEHAVIOR HYPOACTIVE <UNSPECIFIED> RESPIRATION LABORED SKIN & PELAGE PALE ENTIRE BODY RED HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 465 211, 218, 225 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 232, 239, 246 , 253, 260, 267, 274 , 281, 288, 295, 302 , 309 316, 323, 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463 485 485 485 485 485 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 3817 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 35 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92243 M 70 NORMAL NO REMARKABLE OBSERVATIONS C92244 D 65 SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92245 M 68 APPEARANCE MALOCCLUSION THIN OTHER EXCRETION FEW FECES NONFORMED FECES EYES PALE EYES DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484 379, 386, 393, 400, 407, 414, 421, 428 , 435, 442, 449, 453 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 365, 372, 379,, 386, 393, 400,, 414, 421, 428, 435, 441-442 448-449, 456, 463, 470, 472, 476 470, 472, 476 470C 441-442, 448-449, 456, 463, 470, 472, 476 295, 441-442, 448 470 3818 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 36 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92245 M 68 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92246 3 53 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C92247 3 53 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 449 472, 476 1, 8,, 15, 22, 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 302, 309, 316,, 323, 330, 337, 344,. 351, 358, 407 134, 176, 183, 344 1, 8,, 15, 22, 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 141, 148, 155, 162,. 169, 190, 197, 204, 211, 218 225, 232, 239,, 246, 253, 260, 267,. 274, 281, 288, 295, 302, 309 316, 323, 330, 337, 351, 358, 365, 367 1, 8,, 15, 22, 29, 36 , 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309,, 316, 323, 330, 337,. 344, 351, 358, 365, 367 3819 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 37 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92248 M 69 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92249 D 72 EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT 449, 456, 463, 470, 472, 477 477 470, 472, 477 470, 472, 477 449, 456, 463, 470 , 472, 477 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442 295 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 470, 477, 484, 491, 498-499 3820 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 38 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92249 D 72 NORMAL NO REMARKABLE OBSERVATIONS C92250 M 91 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 12 7, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372 637 637 29, 36, 43 , 50 , 57 , 64, 71, 78, 85 624, 631, 637 1, 8 , 15, 22, 92, 99, 106, 113, 120 , 127 , 134 , 141 , 148 , 155 162, 169, 176, 183 , 190, 197, 204, 211, 218, 225, 232, 239, 246 253, 260, 267, 274 , 281,. 288, 295, 302, 309, 316, 323, 330, 337 344, 351, 358, 365 , 372,. 379, 386, 393, 400, 407, 414, 421, 428 435, 442, 449, 456 , 463,. 470, 477, 484, 491, 498, 505, 512, 519 526, 533, 540, 547 , 554,. 561, 568, 575, 582, 589, 596, 603, 610 617 3821 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 39 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92251 M 84 RESPIRATION IRREGULAR SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-RIGHT PAW-HIND-LEFT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 78 T 105 APPEARANCE PROTRUDING PENIS THIN EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS 582 576, 582 449, 456, 463,. 470, 477, 484, 491, 498 , 505, 512, 519, 526 , 533 540, 547, 554, 561, 568 582 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442 540C , 547C , 554C, 561C, 568C, 575- 576C , 582C 708, 715, 722 729- 730 708, 715, 722 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 3822 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 40 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92252 T 105 NORMAL NO REMARKABLE OBSERVATIONS 3 53 NORMAL NO REMARKABLE OBSERVATIONS M 87 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE GENITAL RED IN COLOR NASAL RED IN COLOR EXCRETION FEW FECES NO FECES DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 386, 477, 568, 659, 302, 393, 484, 575, 666, 309, 400, 491, 582, 673, 316 , 323, 407 , 414, 498 , 505, 589 , 596, 680 , 687, 330, 421, 512, 603, 694, 337, 428, 519, 610, 701 344 , 351, 435 , 442, 526 , 533, 617 , 624, 358, 449, 540, 631, 365, 456, 547, 638, 372, 463, 554, 645, 379 470 561 652 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 582- 583, 587, 589, 596, 603 589 386 589, 596, 603 582, 603 583, 587, 589, 596 3823 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 41 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 (CONTINUED FROM PREVIOUS PAGE) C92254 M 87 EYES RED DISCHARGE EYES HOUSING,FOOD,WATER & MAINT. ANIMAL PUT IN PLASTIC CAGE SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92255 2 C92256 2 14 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 583, 587, 589, 596, 603 587 533, 540, 547, 554, 561, 568, 575, 582-583, 587, 589, 596, 603 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155,. 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337,. 344, 351, 358, 365, 372 , 379 393, 400, 407, 414, 421, 428, 435,. 442, 449, 456, 463, 470 , 477 484, 491, 498, 505, 512, 519, 526 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78 , 85, 92-93 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78 , 85, 92-93 3824 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 42 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92257 2 C92258 2 C92259 1 C92260 2 14 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 71, 78, 85, 92-93 1, 8, 15, 22, 29, 36, 43, 50, 57, 64 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 1, 8, 15, 22- 23 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 3825 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 64 MASS(ES) 65 INGUINAL REGION-LEFT WEEK 64 66 MASS(ES) INGUINAL REGION-LEFT WEEK 64 67 MASS(ES) 73 INGUINAL REGION-LEFT WEEK 64 74 MASS(ES) INGUINAL REGION-LEFT WEEK 64 75 MASS(ES) 77 INGUINAL REGION-LEFT WEEK 64 78 MASS(ES) 80 INGUINAL REGION-LEFT WEEK 64 81 MASS(ES) INGUINAL REGION-LEFT WEEK 64 82 MASS(ES) 84 INGUINAL REGION-LEFT WEEK 64 30 X 20MM 30 X 30MM 40 X 35MM 50 X 55MM 60 X 55MM 70 X 55MM 70 X 60MM 75 X 65MM 3826 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT M8 589 85 TO 596 86 MASS(ES) INGUINAL REGION-LEFT WEEK 64 603 87 MASS(ES) INGUINAL REGION-LEFT WEEK 64 610 88 TO 617 89 MASS(ES) INGUINAL REGION-LEFT WEEK 64 624 90 TO 638 92 MASS(ES) INGUINAL REGION-LEFT WEEK 64 645 93 TO 659 95 MASS(ES) INGUINAL REGION-LEFT WEEK 64 666 96 MASS(ES) INGUINAL REGION-LEFT WEEK 64 673 97 MASS(ES) TO 694 100 INGUINAL REGION-LEFT WEEK 64 701 101 MASS(ES) INGUINAL REGION-LEFT WEEK 64 75 X 70MM 75 X 75MM 75 X 90MM 80 X 90MM 90 X 90MM 100 X 90MM 110 X 90MM 130 X 100MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 44 3827 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92191 C92202 C92217 M8 708 102 MASS(ES) INGUINAL REGION-LEFT WEEK 64 715 103 MASS(ES) TO 722 104 INGUINAL REGION-LEFT WEEK 64 M8 592 85 DISCHARGE OTHER M8 225 33 TO 246 36 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 33 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 442 64 TO 456 66 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 463 67 TO 491 71 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 498 72 TO 505 73 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 50 X 50MM 135 X 100MM RED DISCHARGE 15 X 15MM 10 X 10MM 50 X 40MM 60 X 60MM 70 X 70MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 45 3828 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92217 C92219 C92222 C92223 M8 512 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 519 75 TO 533 77 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 540 78 TO 547 79 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 63 M8 607 87 BEHAVIOR TO 607 87 OTHER M8 729 105 MASS(ES) TO 730 105 DORSAL WEEK 95 519 75 MASS(ES) VENTRAL-ABDOMINAL WEEK 75 M8 274 40 TO 288 42 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 40 211 31 TO 218 32 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 28 80 X 80MM 120 X 100MM 140 X 100MM ANIMAL IS CHEWING ON DISTAL PORTION OF TAIL 15 X 10MM ANTERIOR 10 X 10MM POSTERIOR 20 X 20MM 15 X 15MM 15 X 15MM Covance 6329-228 3M T-6316.1 PAGE: 46 3829 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 47 STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92224 C92245 C92251 M8 218 32 TO 246 36 M8 470 68 M8 540 78 TO 547 79 554 80 561 81 TO 582 84 MASS(ES) VENTRAL-ABDOMINAL WEEK 32 APPEARANCE OTHER MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 78 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 78 10 X 10MM EXCESSIVE BLEEDING FROM PAWS, CAUTERIZED WITH SILVER NITRATE 35 X 35MM 40 X 35MM 80 X 60MM 3830 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 48 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92261 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92262 M MASS(ES) VENTRAL-ABDOMINAL WEEK 76 104 APPEARANCE THIN SKIN & PELAGE ALOPECIA PALE ENTIRE BODY SORE/SCAB DORSAL HEAD-ENTIRE VENTRAL-CERVICAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 540 , 547, 554, 561, 568 , 575 582, 589, 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694 , 701, 708, 715, 722 , 729- 730 526, 533 659, 666 673, 680, 687, 694, 701, 708, 715, 722 708, 715 708, 715 638, 645 645, 652 610, 617 722 722 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 624, 631 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 3831 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 49 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92262 M 104 NORMAL NO REMARKABLE OBSERVATIONS C92263 T 105 APPEARANCE MALOCCLUSION DISCHARGE GENITAL RED IN COLOR EXCRETION NONFORMED FECES SKIN & PELAGE RED HAIR COAT DORSAL SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) TAIL-PROXIMAL WEEK 64 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603 337, 344, 351, 358, 365, 372, 638, 645, 652, 659, 666, 673 701 722, 729730 694, 701, 708,. 715, 722 547, 554, 561,. 568, 575, 582, 589, 596 , 603, 610, 617, 624 , 631 708, 715, 722 1, 8 113, 204, 295, 428, 15, 120, 211, 302, 435 22, 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 379, 386 , 393, 400, 92, 99, 106 183, 190 , 197 274, 281 , 288 407, 414 , 421 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512, 519, 526 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610, 617 3832 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 50 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92263 T 105 WART LIKE LESION(S) TAIL-PROXIMAL WEEK 64 C92264 1 C92265 D 4 NORMAL NO REMARKABLE OBSERVATIONS 80 APPEARANCE CONVULSIONS MALOCCLUSION MISSING TEETH DISCHARGE CLEAR ORAL <UNSPECIFIED> MODERATE EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 624, 631, 638 , 645 , 652, 659 , 666 , 673 , 680, 687, 694 , 701 , 708 715, 722, 729 -730 1, 8 , 15, 22- 23 540 57, 64, 71., 78, 85 , 92, 99, 106, 113, 120, 127, 134, 141, 148 155, 162, 169 , 176 , 183, 190 , 197 , 204 , 211, 218, 225 , 232 , 239 246, 253, 260 , 267 , 274, 281 , 288 , 295 , 302, 309, 316 , 323 , 330 337, 344, 351 , 358 , 365, 372 , 379 , 386 , 393, 400, 407 , 414 , 421 428, 435, 442 , 449 , 456, 463 , 470 , 477 , 484, 491, 498 , 505 , 512 519, 526, 533 , 540 , 547, 554 365, 372, 379 , 386 , 393, 400 , 407 , 414 , 421, 428, 435 , 442 , 449 456, 463, 470 , 477 , 484, 491 , 498 , 505 , 512, 519, 526 , 533 , 540 547, 554 540 253, 260 134, 204, 498 , 505 1, 8 , 15, 22, 29, 36, 43 , 50 3833 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 51 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92266 T 105 DISCHARGE GENITAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS C92267 3 WART LIKE LESION(S) DORSAL WEEK 62 WEEK 76 VENTRAL -ABDOMINAL WEEK 92 53 NORMAL NO REMARKABLE OBSERVATIONS 141 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 148, 155, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407, 414 , 421, 519 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 526, 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603, 610 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694, 701 708, 715, 722, 729- 730 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729-730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 3834 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 52 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92268 M 100 APPEARANCE OBESE THIN EXCRETION NONFORMED FECES SKIN & PELAGE SORE/SCAB DORSAL-THORACIC-LEFT SCROTUM-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 72 519, 526, 533,. 540, 547, 554, 561, 568 , 575, 582, 589, 596 , 603 610, 617, 624, 631 694 204 638, 645, 652, 659 666, 673, 680, 687, 694 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 211, 218, 225,. 232, 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316,. 323, 330, 337, 344, 351 , 358, 365, 372, 379 , 386 393, 400, 407,. 414, 421, 428, 435, 442 , 449, 456, 463, 470 , 477 484, 491 498, 505, 512 3835 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 53 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92269 D 73 NORMAL NO REMARKABLE OBSERVATIONS C92270 T 105 EXCRETION NONFORMED FECES SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 40 1, 8,r 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505- 506 624, 631 694 295, 302, 442, 449, 701 1, 8,r 15, 113, 120, 204, 211, 330, 337, 421, 428, 526, 533, 617, 638, 729-730 22, 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 344, 351, 358, 365, 435,. 456, 463, 470, 540, 547, 554, 561, 645,. 652, 659, 666, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 372, 379 , 386, 393, 477, 484 , 491, 498, 568, 575 , 582, 589, 673, 680 , 687, 708, 92, 99, 106 183, 190 , 197 309, 316 , 323 400, 407 , 414 505, 512 , 519 596, 603 , 610 715, 722 274C, 281C, 288C 3836 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 54 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92271 D 90 NORMAL NO REMARKABLE OBSERVATIONS C92272 M MASS(ES) DORSAL WEEK 90 85 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 74 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 624-625C 519, 526, 533 , 540, 547, 554, 561, 568, 575, 582- 583, 589-590 582- 583, 589- 590 568, 575 1, 8 , 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36 , 43,r 50, 127 , 134, 141, 148, 218 , 225, 232, 239, 309 , 316, 323, 330, 400 , 407, 414, 421, 491 , 498, 505 57, 64, 71 , 78, 85, 155, 162, 169, 176, 246, 253, 260, 267, 337, 344, 351, 358, 428, 435, 442, 449, 92, 99, 106 183 , 190, 197 274 , 281, 288 365 , 372, 379 456 , 463, 470 512, 519C , 526C, 533C, 540C, 54 7C, 554C, 561C , 568C, 575C 582- 583C, 589 -590C 3837 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 55 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92273 D 42 NORMAL NO REMARKABLE OBSERVATIONS C92274 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92275 M 104 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EYES SQUINTED PERI-ORBITAL SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218,. 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 294 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218,. 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337,. 344, 351, 358, 365, 367 701, 708, 715, 722 722 722 701, 708 715, 722 1, 8, 15 113, 120 204, 211 295, 302 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 3838 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 56 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92275 M 104 NORMAL NO REMARKABLE OBSERVATIONS C92276 D MASS(ES) INGUINAL REGION-LEFT WEEK 78 73 APPEARANCE THIN SKIN & PELAGE RED HAIR COAT DORSAL-CERVICAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92277 M 60 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT STERNAL EXCRETION NO FECES DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 582, 589, 596, 603 610, 617, 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 540C, 547C, 554C, 561C, 568C, 575C 498, 505, 510 505, 510 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491 415 415 414-415 3839 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 57 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92277 M 60 SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS T 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C92279 M 89 APPEARANCE HEAD TILT RIGHT HUNCHED SWOLLEN PAW-HIND-RIGHT DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 414-415 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 288, 295, 463,. 470, 582, 589, 596, 603 , 610, 617, 624, 631 , 638 645, 652, 659,. 666, 673, 680, 687,. 694 , 722, 729- 730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 302 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 421, 428, 435, 442, 449 , 456, 477, 484, 491 , 498 505, 512, 519,. 526, 533, 540, 547, 554 , 561, 568, 575, 701 , 708 715 614, 617 614, 617 568, 575, 582, 589, 596, 603 3840 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 58 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92279 M 89 BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE GENITAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 46 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 610, 614, 617 316, 603, 614 610, 614, 617 155 337, 344, 351 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 358, 365 , 372, 379, 386, 393 , 400, 407, 414, 421 , 428 435, 442, 449, 456 , 463, 470, 477, 484 , 491, 498, 505, 512 , 519 526, 533, 540, 547 , 554, 561 316C, 323C, 330C 3841 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 59 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92280 1 C92281 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C92282 T MASS(ES) VENTRAL-ABDOMINAL WEEK 45 105 EXCRETION NONFORMED FECES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 652 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 337,. 344, 351, 358, 365, 372 , 379, 386, 393, 400 , 407 414, 421, 428, 435, 442, 449, 456, 463 , 470, 477, 484, 491 , 498 505, 512, 519,. 526, 533, 540, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617, 624, 631, 638, 645 , 659, 666, 673, 680 , 687 694, 701, 708,. 715, 722, 729- 730 309C, 316C, 323C, 330C 288, 295, 463,. 470, 729- 730 582, 589, 596, 603, 610, 617, 624, 631 , 638, 645, 652, 659 , 666 673, 680, 687,. 694, 701, 708, 715, 722 , 729- 730 1, 8,r 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 302 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372, 379, 386 , 393 3842 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 60 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92282 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92283 T WART LIKE LESION(S) LIMB-FRONT-RIGHT WEEK 105 105 EXCRETION NONFORMED FECES SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT RED HAIR COAT DORSAL-CERVICAL SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) TAIL-MID WEEK 101 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 400, 407, 414, 421, 428 435, 442, 449, 456, 477, 484, 491, 498 505, 512, 519, 526, 533 540, 547, 554, 561, 568, 575 729-730 638 694 442, 449, 456, 463 701, 708 1, 8,r 15, 22, 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 470, 477, 484, 491 , 498 505, 512, 519,. 526, 533, 540, 547, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617, 624, 631, 645, 652 , 659, 666, 673, 680 , 687 701, 708, 715,. 722, 729- 730 3843 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 61 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92284 1 C92285 M 4 NORMAL NO REMARKABLE OBSERVATIONS 76 BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92286 3 53 SKIN & PELAGE SORE/SCAB MOUTH NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 519, 526-527 526-527 519, 526-527 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512 8, 15, 22 1, 29, 36,r 43, 50, 57, 64 , 71,, 78, 85, 92, 99, 106, 113 , 120 127 , 134, 141, 148,. 155, 162, 169, 176, 183, 190, 197, 204, 211 218 , 225, 232, 239,. 246, 253, 260, 267, 274, 281, 288, 295, 302 309 , 316, 323, 330, 337, 344, 351, 358, 365, 367 3844 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 62 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92287 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92288 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92289 T 105 APPEARANCE MALOCCLUSION THIN SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 64 701, 708, 715, 722 638, 645 652, 659, 666, 673, 680, 687, 694, 701, 708, 715, 722 729- 730 386, 393 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 575, 582 589, 596, 603, 610, 617 1, 8 , 15 120, 127 211, 218 302, 309 407, 414 22, 29, 36, 43,r 50, 57, 71, 78, 85,, 92, 99, 106, 113 134, 141 , 148, 155, 162, 169 , 176, 183, 190, 197, 204 225, 232 , 239, 246, 253, 260 , 267, 274, 281,. 288, 295 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379, 400 421, 428 , 435, 442, 449, 456 , 463, 470, 477,. 624, 631 3845 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 63 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92289 T 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 79 C92290 M 63 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES RESPIRATION IRREGULAR SKIN & PELAGE ROUGH HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 547, 554, 561, 568, 575, 582, 589, 596, 603 421, 428, 431, 435, 438 421, 428, 431, 435, 438 438 438 414, 421, 428, 431, 435 438 438 428, 431 435, 438 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 3846 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 64 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92291 D 97 EXCRETION NONFORMED FECES EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS M 67 APPEARANCE HUNCHED THIN BEHAVIOR SENSITIVE TO TOUCH EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL RESPIRATION IRREGULAR 302, 309 442 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 316, 323, 330 , 337, 344, 351, 358 , 365, 372, 379, 386 , 393 400, 407, 414, 421 , 428, 435, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519 , 526, 533, 540, 547 , 554, 561, 568, 575 , 582 589, 596, 603, 610 , 617, 624, 631, 638 , 645, 652, 659, 666 , 673 442, 449, 456, 462 -463 421, 428, 435, 442 , 449, 456, 462- 463 462-463 462-463 462-463 462-463 3847 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 65 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92292 M 67 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92293 D 42 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C92294 M 97 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION NO FECES DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 442, 449, 456, 462-463 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414 260 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218,. 225, 232, 239, 246, 253 , 267, 274, 281, 288 638, 645, 652,. 659, 666, 668, 673 673 673 668, 673 3848 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 66 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92294 M 97 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92295 T 105 EYES OPAQUE EYES PALE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 666, 668, 673 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 668, 673 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 638, 645, 652 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582 589, 596, 603, 610, 617 , 624, 631 3849 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 67 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92296 M 75 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL SORE/SCAB VENTRAL-THORACIC NORMAL NO REMARKABLE OBSERVATIONS WART LIKE LESION(S) VENTRAL-ABDOMINAL WEEK 58 519, 525 525 519 525 525 519, 525 498, 505 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 463, 470 , 477, 484, 491, 512 400, 407, 414, 421 , 428, 435, 442, 449 , 456 3850 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 68 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92297 T 105 EYES SCAB EYE-LEFT SQUINTED PERI-ORBITAL SKIN & PELAGE SORE/SCAB LIMB-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92298 M 68 APPEARANCE HUNCHED MALOCCLUSION THIN BEHAVIOR SENSITIVE TO TOUCH EXCRETION FEW FECES NO FECES 701, 708 638, 645, 652 715, 722, 729-730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 659, 666 , 673 680, 687, 694 449, 456, 462-463, 470 442, 449, 456, 462-463, 470 449, 456, 462-463, 470 462-463 442, 449, 456 462-463, 470 3851 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 69 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92298 M 68 SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92299 T SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 470 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 694, 697, 701, 708 694, 697, 701, 708 715, 722, 729- 730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687 3852 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 70 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92300 1 C92301 D 4 NORMAL NO REMARKABLE OBSERVATIONS 87 NORMAL NO REMARKABLE OBSERVATIONS C92302 M 96 BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT STERNAL DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES EYES SQUINTED PERI-ORBITAL RESPIRATION LABORED 1, 8, 15 22-23 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596, 603, 605 668 668 666, 668 666 668 668 668 3853 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 71 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92302 M 96 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS C92303 3 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 53 NORMAL NO REMARKABLE OBSERVATIONS M 96 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 666, 668 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 547 , 554, 561, 568, 575 , 582 589, 596, 603,. 610, 617, 624, 631, 638 , 645, 652, 659 526, 533, 540 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 367 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582,. 589, 596, 603, 610, 617 , 624, 631, 638 3854 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 72 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92304 M 96 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 93 C92305 M 63 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES RESPIRATION LABORED SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL HEAD-ENTIRE VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 64 5C, 652C, 659C, 666C 438 421, 438 438 438 438 106 438 414, 421, 428, 435, 438 428, 435, 438 400, 407, 414, 421, 428, 435, 438 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113 120, 127, 134, 141 , 148, 155, 162, 169 , 176, 183, 190, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 302, 309, 316, 323 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 3855 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 73 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92305 M 63 NORMAL NO REMARKABLE OBSERVATIONS C92306 D 104 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS M APPEARANCE THIN BEHAVIOR AGGRESSIVE <UNSPECIFIED> MODERATE SENSITIVE TO TOUCH RESPIRATION AUDIBLE IRREGULAR NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 393 , 645, 652 8, 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 , 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 , 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 , 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 , 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 , 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 , 575, 582, 589, 596, 603, 610, 617 , 624, 631, 659, 666 , 673 , 687, 694, 701, 708, 715, 722 , 645, 652, 659, 666, 673, 680, 687 , 694, 701, 706- 707 , 204, 211,. 218, 225, 232, 239, 246 , 253, 260, 267, 274 , 281 , 295, 302, 309, 316, 323, 330 , 148, 155,. 162, 169, 176, 183, 190 134 701, 706-707 701, 706-707 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 3856 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 74 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92307 M 101 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) HEAD-MAXILLARY-LEFT WEEK 100 WART LIKE LESION(S) DORSAL WEEK 59 C92308 T MOUTH WEEK 100 NOSE WEEK 100 105 APPEARANCE PARALYSIS LIMB-FRONT-LEFT THIN SKIN & PELAGE SORE/SCAB DORSAL-CERVICAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 694, 701, 706-707 407, 414, 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575, 582 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666, 673 680, 687, 694, 701, 706- 707 694 694, 701, 706-707 50 729-730 29, 36, 43 155 1, 8,, 15, 22, 57, 64 , 71,, 78, 85, 92, 99, 106, 113 , 120 , 127 134, 141, 14 8,. 162, 169, 176, 183,. 190, 197, 204, 211, 218, 225 232, 239, 246,. 253, 260, 267, 274,. 281, 288, 295, 302, 309, 316 3857 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 75 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92308 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92309 T 105 APPEARANCE THIN NORMAL NO REMARKABLE OBSERVATIONS D 73 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 323, 414, 505, 596, 687, 330, 421, 512, 603, 694, 337, 428, 519, 610, 701, 344, 435, 526, 617, 708, 351, 442, 533, 624, 715, 358, 449, 540, 631, 722 365, 456, 547, 638, 372, 463, 554, 645, 379, 470, 561, 652, 386, 477, 568, 659, 393, 484, 575, 666, 400, 491, 582, 673, 407 498 589 680 729-730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 507 3858 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 76 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92311 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92312 M 65 APPEARANCE HUNCHED MALOCCLUSION PROTRUDING PENIS THIN EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SKIN & PELAGE ROUGH HAIR COAT SORE/SCAB PAW-HIND-LEFT 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680 , 687, 694, 701, 708 , 715, 722, 729- 730 316, 407, 407, 431 302, 393, 323, 414, 414, 309, 400, 330, 421, 421, 316, 407, 337 , 344, 428 , 431, 428 , 431, 323 , 330, 414 , 421, 351, 435, 435, 337, 428, 358, 442, 442, 344, 431, 365 , 372, 449 449 351 , 358, 435 , 442, 379, 365, 449 386, 372, 393 , 400 379 , 386 449 246, 253 267, 274, 281, 288 , 295, 302, 309, 344 , 351, 358, 365, 372 , 379 386, 393, 400 316, 323, 330, 337 414, 421, 428, 431 , 435, 442, 449 379, 386, 393, 400 , 407, 414, 421, 428 , 431, 435, 442, 449 3859 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 77 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92312 M 65 NORMAL NO REMARKABLE OBSERVATIONS C92313 D 97 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225 , 232, 239, 260 204, 211, 512, 547 , 554, 561 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155,. 162, 169, 176, 183,. 190, 197 218, 225, 232, 239 , 246, 253, 260,. 267, 274, 281, 288, 295, 302 309, 316, 323, 330 , 337, 344, 351,. 358, 365, 372, 379,. 386, 393 400, 407, 414, 421 , 428, 435, 442,. 449, 456, 463, 470, 477, 484 MASS(ES) DORSAL WEEK 72 WART LIKE LESION(S) DORSAL WEEK 72 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652C, 659C, 666C, 673-674C 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568 3860 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 78 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92314 M 93 APPEARANCE MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS 643, 645, 649 638, 643, 645, 649 649 638, 643, 645, 649 643, 645, 649 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 643, 645 , 649 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568 3861 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 79 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92315 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92316 M 101 APPEARANCE HUNCHED EYES PALE EYES SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 48 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 701-702 694, 701-702 610, 702 337, 344 428, 435 519, 526 610, 617 701- 702 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 533, 540, 547, 554, 561, 568, 575, 582, 589, 596, 603 624, 631, 638, 645, 652, 659, 666, 673, 680, 687, 694 1, 8 , 15 113, 120 204, 211 295, 302 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 309,. 316, 323 330, 337 344, 351, 358, 365, 372, 379 3862 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 80 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92316 M 101 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 C92317 3 WART LIKE LESION(S) DORSAL WEEK 82 53 NORMAL NO REMARKABLE OBSERVATIONS C92318 T 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701-702C 568 1, 8, 15 113, 120 204, 211 295, 302 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 309, 316, 323, 330, 337, 344, 351, 358, 365, 367 498, 505 722, 729-730 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 582, 589 673, 680 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 512 , 519, 526, 533, 540 , 547, 554, 561, 568 , 575 596, 603 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 687,. 694 , 701, 708, 715 3863 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 81 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92319 T 105 APPEARANCE MALOCCLUSION EXCRETION FEW FECES NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-THORACIC-RIGHT WEEK 82 WART LIKE LESION(S) VENTRAL-THORACIC-RIGHT WEEK 82 113, 204, 295, 386, 477, 568, 659, 120, 211, 302, 393, 484, 575, 666, 127,. 134, 218, 225, 309,. 316, 400, 407, 491, 498, 582, 589, 673, 680, 141, 232, 323, 414, 505, 596, 687, 148, 239, 330, 421, 512, 603, 694, 155, 246, 337, 428, 519, 610, 701, 162 , 169, 253 , 260, 344 , 351, 435 , 442, 526 , 533, 617 , 624, 708 , 715, 176, 267, 358, 449, 540, 631, 722, 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 645 , 652 729- 730 414, 421, 428, 435, 442, 449, 456, 463 498, 505 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 575, 582, 589,. 596, 603, 610, 617, 624 , 631, 638, 645, 652 , 659 666, 673, 680, 687, 694, 701, 708, 715 , 722, 729- 730C 568 3864 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 82 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92320 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92321 T 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C92322 T MASS(ES) VENTRAL-ABDOMINAL WEEK 105 105 NORMAL NO REMARKABLE OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 729- 730 1, 8 , 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715 722C 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 638, 645, 652, 659, 666, 673 , 680, 687, 694, 701 , 708 3865 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 83 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92322 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 84 C92323 T 105 APPEARANCE LIMITED USE LIMB-HIND-LEFT THIN SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL VENTRAL-THORACIC-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 76 WEEK 84 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 715, 722, 729-730 582C,, 589C, 596C, 603C, 610C, 617C, 624C 724, 729-730 724 575 708, 715 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 722 526, 533, 540, 547, 554, 561, 568 582C, 589C , 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C , 673C, 680C, 687C, 694C 3866 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 84 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 (CONTINUED FROM PREVIOUS PAGE) C92323 T 105 WART LIKE LESION(S) VENTRAL-ABDOMINAL-RIGHT WEEK 82 VENTRAL-THORACIC-RIGHT WEEK 101 C92324 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92325 2 C92326 2 C92327 2 C92328 2 C92329 1 14 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 568 701 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344 , 351, 358, 365, 367 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92-93 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92-93 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92-93 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92-93 1, 8,, 15, 22-23 3867 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 85 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: M9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 2 14 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78 3868 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 40 MASS(ES) 42 VENTRAL-ABDOMINAL WEEK 40 90 MASS(ES) 90 DORSAL WEEK 90 75 MASS(ES) HEAD-MAXILLARY-RIGHT WEEK 74 76 MASS(ES) 81 HEAD-MAXILLARY-RIGHT WEEK 74 82 MASS(ES) 83 HEAD-MAXILLARY-RIGHT WEEK 74 84 MASS(ES) 85 HEAD-MAXILLARY-RIGHT WEEK 74 78 MASS(ES) 83 INGUINAL REGION-LEFT WEEK 78 46 MASS(ES) 48 VENTRAL-ABDOMINAL-LEFT WEEK 46 20 X 30MM 10 X 10MM 10 X 10MM 20 X 15MM 30 X 30MM 30 X 40MM 10 X 10MM 10 X 10MM 3869 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 45 MASS(ES) 48 VENTRAL-ABDOMINAL WEEK 45 93 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 93 94 MASS(ES) 95 HEAD-MAXILLARY-LEFT WEEK 93 96 MASS(ES) 96 HEAD-MAXILLARY-LEFT WEEK 93 96 MASS(ES) HEAD-MAXILLARY-LEFT WEEK 93 83 MASS(ES) DORSAL WEEK 72 84 MASS(ES) 91 DORSAL WEEK 72 92 MASS(ES) 95 DORSAL WEEK 72 10 X 10MM 20 X 30MM 30 X 30MM 35 X 30MM 30 X 30MM 20 X 20MM 30 X 20MM 40 X 30MM 3870 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92313 C92316 C92319 C92321 C92322 M9 666 96 TO 674 97 MASS(ES) DORSAL WEEK 72 M9 582 84 TO 617 89 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 624 90 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 666 96 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 673 97 MASS(ES) TO 702 101 VENTRAL-ABDOMINAL-RIGHT WEEK 84 M9 729 105 MASS(ES) TO 730 105 VENTRAL-THORACIC-RIGHT WEEK 82 M9 722 104 MASS(ES) VENTRAL-ABDOMINAL WEEK 105 M9 582 84 TO 631 91 MASS(ES) VENTRAL-ABDOMINAL WEEK 84 35 X 40MM 20 X 20MM 25 X 25MM 30 X 30MM 30 X 40MM 10 X 10MM 10 X 10MM 15 X 20MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 88 3871 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92323 M9 582 84 MASS(ES) TO 694 100 VENTRAL-ABDOMINAL-RIGHT WEEK 84 10 X 10MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 89 3872 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 90 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92331 M 92 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES NO FECES EYES SQUINTED PERI-ORBITAL RESPIRATION IRREGULAR SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 42 638, 641 638, 641 641 641 638 641 641 638, 641 638, 641 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78,, 85, 92, 99, 106 113, 120, 12 7, 134 , 141, 148, 155, 162, 169, 176, 183,. 190, 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 288C, 295C , 302C, 309C, 316C, 323C , 330C, 337C, 344C, 351C, 358C 3873 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 91 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92331 M 92 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 C92332 1 C92333 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 56 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 641C 1, 8, 15, 22-23 393, 400 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 407, 414, 421,. 428, 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498, 505, 512, 519, 526, 533, 540, 547 , 554, 561, 568, 575 , 582 589, 596, 603,. 610, 617, 624, 631, 638 , 645, 652, 659, 666 , 673 680, 687, 694, 701, 708, 715, 722, 729 -730 386, 393, 400 3874 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 92 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92334 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92335 M C92336 M 8 APPEARANCE SWOLLEN MOUTH NORMAL NO REMARKABLE OBSERVATIONS 82 SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 59 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 54 1, 8, 15 22, 29, 36, 43, 50 554, 561 568, 570 568, 570 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400 407C, 414C, 421C, 428C, 435C, 442C, 449C, 456C, 463C, 470C, 477C 3875 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 93 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92336 M 82 MASS(ES) AXILLARY REGION-LEFT WEEK 59 AXILLARY REGION-RIGHT WEEK 75 C92337 M 82 APPEARANCE HEAD TILT LEFT DISCHARGE OTHER EYES RED DISCHARGE EYE-LEFT EYE-RIGHT SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 76 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 540C, 547C, 554C 561C, 568C, 570C 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 570C 569 274C 554, 561, 568-569 512, 519 519 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 281,. 288, 295 302, 309, 316,. 323, 330, 337 526, 533, 540, 547, 554, 561, 568-569 3876 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 94 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92337 M 82 MASS(ES) VENTRAL-ABDOMINAL WEEK 50 C92338 M VENTRAL-ABDOMINAL-LEFT WEEK 73 70 BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE ALOPECIA RED HAIR COAT VENTRAL-ABDOMINAL VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 344C, 351C, 358C, 365C, 372C, 379C, 386C, 393C, 400C, 407C, 414C 421C, 42 8C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C 498C, 505C, 512C, 519C, 52 6C, 53 3C, 54 0C, 54 7C, 554C, 561C 568-569C 505C, 512C 484 484 483-484 344 483- 484 483- 484 1, 8 , 15 113, 120 204, 211 295, 302 393, 400 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 351 , 358, 365, 372, 379 , 386 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 , 477 3877 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 95 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92339 D 94 EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) SHOULDER-LEFT WEEK 38 VENTRAL-ABDOMINAL-RIGHT WEEK 66 C92340 M 79 SKIN & PELAGE BLACK SKIN VENTRAL-ABDOMINAL VENTRAL-THORACIC-LEFT NORMAL NO REMARKABLE OBSERVATIONS 260, 267, 274,. 379, 386, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 365, 372 , 393, 400 , 407 414 260C, 267C , 274C, 281C, 288C, 295C , 302C, 309C, 316C, 323C , 330C 337C, 344C, 351C, 358C 456, 463, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 533C, 540C, 547C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 610C, 617C, 624C, 631C, 638C, 645C, 652C 512, 519, 526, 533 , 540, 547548 512, 519, 526, 533 , 540, 547- 548 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155,. 162, 169, 176, 183,. 190, 197 204, 211, 218, 225 , 232, 239, 246,. 253, 260, 267, 274, 281, 288 295 3878 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 96 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92340 M 79 MASS(ES) INGUINAL REGION-LEFT WEEK 79 VENTRAL-ABDOMINAL WEEK 74 VENTRAL-ABDOMINAL-LEFT WEEK 44 VENTRAL-THORACIC-LEFT WEEK 74 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92342 M MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 50 APPEARANCE MALOCCLUSION THIN DISCHARGE NASAL RED IN COLOR DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 547-548C 512C, 519C, 526C, 533C, 54 0C, 547- 548C 302, 309, 316, 323, 330, 337, 344C , 351C , 358C, 365C, 372C , 379C 386C,. 393C, 400C, 407C, 414C, 421C , 428C , 435C, 442C, 449C , 456C 463C,. 470C, 477C, 484C, 491C, 498C , 505C , 512C, 519C, 526C , 533C 54 0C, 547-548C 512C, 519C, 526C, 533C, 54 0C, 547- 548C 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 365C, 36 120, 127 211, 218 302, 309 344 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204 225, 232, 239, 246, 253, 260, 267, 274, 281, 288, 295 316, 323, 330, 337, 344 120 3879 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 97 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92342 M 50 EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT RESPIRATION AUDIBLE SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS C92343 M MASS(ES) AXILLARY REGION-RIGHT WEEK 40 58 SKIN & PELAGE PALE ENTIRE BODY SORE/SCAB SHOULDER-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 344 120, 127, 134,141 344 120, 344 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113 274, 281, 288,, 295, 302, 309, 316,, 323, 330, 337 351, 358 365, 372, 379,, 386, 393, 400 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 3880 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 98 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92343 M 58 MASS(ES) SHOULDER-RIGHT WEEK 19 M 104 APPEARANCE HUNCHED MALOCCLUSION BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 12 7C , 134C , 141C, 148C, 155C, 162C , 169C, 176C, 183C, 190C , 197C 204C , 211C , 218C, 225C, 232C, 239C , 246C, 253C, 260C, 267C , 274C 281C , 288C , 295C, 302C, 309C, 316C , 323C, 330C, 337C, 344C , 351C 358C , 365C , 372C, 379C, 386C, 393C , 400C 721- 722 638, 645, 652,. 659, 666, 673, 680, 687, 694, 701, 708, 715 721- 722 721- 722 582, 589, 596, 603 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78,, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554 , 561 568, 575, 610,. 617, 624, 631 3881 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 99 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92345 D 30 NORMAL NO REMARKABLE OBSERVATIONS C92346 M 63 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE ROUGH HAIR COAT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 127, 134, 141, 148, 155, 162, 169, 176, 183, 190, 197 204, 206 428, 435 435 435 393, 400 407 435 428, 435 393, 400 407 1, 8, 15 113, 120 204, 211 295, 302 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 309 3882 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 100 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92346 M 63 WART LIKE LESION(S) NOSE WEEK 46 C92347 M 48 APPEARANCE THIN RESPIRATION LABORED SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS T 105 APPEARANCE MALOCCLUSION EXCRETION NONFORMED FECES DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 316, 323, 330, 337, 344, 351, 358, 365, 372, 379, 386, 393, 400 407, 414, 421, 428, 435 330 330 323, 330 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 106, 197, 295, 386, 477, 568, 659, 113, 204, 302, 393, 484, 575, 666, 120, 127, 211,. 218, 309,. 316, 400, 407, 491, 498, 582, 589, 673, 680, 134, 225, 323, 414, 505, 596, 687, 141, 232, 330, 421, 512, 603, 694, 148, 246, 337, 428, 519, 610, 701, 155 , 162, 253 , 260, 344 , 351, 435 , 442, 526 , 533, 617 , 624, 708 , 715, 169, 267, 358, 449, 540, 631, 722, 176, 183 , 190 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 638, 645 , 652 729- 730 414 3883 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 101 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92348 T 105 SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 101 VENTRAL-ABDOMINAL WEEK 100 C92349 D 78 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 701, 708, 715,, 722, 729-730 1, 8,, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92 99, 239 701C, 708C, 715C, 722C, 729-730C 694C, 701C, 708C, 715C, 722C, 729-730C 512, 519, 52 6,. 533, 540- 541 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183 190, 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274 281, 288 295, 302, 309,. 316, 323, 330, 337, 407, 414, 421 , 428 435, 442 449, 456, 463,. 470, 477, 484, 491, 498, 505 344C, 351C , 358C, 365C, 372C, 379C , 386C, 393C, 400C 3884 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 102 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92350 M 94 APPEARANCE HEAD TILT RIGHT DISCHARGE NASAL RED IN COLOR OTHER EYES CLEAR DISCHARGE EYE-LEFT RED DISCHARGE EYES RESPIRATION AUDIBLE LABORED SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS 656 656 656C 638, 645 652 655- 656 656 656 655- 656 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596, 603, 610, 617 , 624, 631 3885 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 103 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92351 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92352 1 C92353 M 4 NORMAL NO REMARKABLE OBSERVATIONS 96 APPEARANCE HEAD TILT LEFT MALOCCLUSION BEHAVIOR ATAXIC MODERATE HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 1, 8, 15, 22-23 666-667 659 666-667 638, 645, 652, 658-659 666-667 658-659, 666-667 638, 645, 652, 666-667 658-659, 666 3886 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 104 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92353 M 96 EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL RESPIRATION IRREGULAR LABORED SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL WARM TO TOUCH ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 658 658 638 667 658-659, 666-667 667 1, 8,r 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 624, 631 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 3887 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 105 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92354 T 105 APPEARANCE HEAD TILT LEFT BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SORE/SCAB AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 83 724, 729-730 724, 729-730 724 701, 708, 715, 722 , 724, 729- 730 701, 708, 715, 722 , 724, 729- 730 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372, 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463, 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540 , 547, 554, 561 568 575, 582, 589, 596 , 603, 610C , 617C, 624C, 631C, 638C, 645C 652C, 659C , 666C, 673C, 680C, 687C , 694C , 701C, 708C, 715C, 722C 724C 3888 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 106 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92355 1 C92356 3 4 NORMAL NO REMARKABLE OBSERVATIONS 53 APPEARANCE MALOCCLUSION C92357 3 DISCHARGE NASAL RED IN COLOR NORMAL NO REMARKABLE OBSERVATIONS 53 NORMAL NO REMARKABLE OBSERVATIONS C92358 T 105 SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22-23 57, 64, 71, 78, 85, 92, 99, 106, 113, 120, 127, 134, 141, 148 155, 162 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239 246, 253 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330 337, 344 351, 358, 365, 367 64 1, 8, 15 22, 29, 36, 43, 50 1, 8, 15 113, 120 204, 211 295, 302 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 365, 372 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 456, 463 470, 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 547, 554 561, 568, 575, 701, 708, 715, 722, 729- 730 1, 8, 15 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 3889 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 107 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92358 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 84 C92359 D 93 APPEARANCE THIN EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 92 AXILLARY REGION-RIGHT WEEK 79 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309,, 316, 323, 330, 337, 344, 351, 358 582, 589, 596, 603, 610C, 617C , 624C, 631C, 638C , 645C, 652C 659C , 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 729- 730C 638, 644-645, 648 638, 644-645, 648 644- 645, 648 1, 8 , 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43,r 50, 127, 134 , 141, 148, 218, 225 , 232, 239, 309, 316 , 323, 330, 400, 407 , 414, 421, 491, 498 , 505, 512, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 638C, 644-645C, 648C 54 7C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C 3890 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 108 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92359 D 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 VENTRAL-ABDOMINAL WEEK 80 C92360 T 105 APPEARANCE MALOCCLUSION SWOLLEN HEAD-MAXILLARY-RIGHT SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT BLACK SKIN VENTRAL-ABDOMINAL SORE/SCAB MOUTH PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 77 WEEK 96 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 624C, 631C, 638C, 644-645C, 648C 554C, 561C, 56 8C, 575C 701 701 701, 708, 715,. 722, 729- 730 428, 435, 442, 449, 456 701, 708 701, 708, 715,. 722, 729- 730 1, 8,, 15, 22, 29, 36 , 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155 , 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246 , 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337 , 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414 533, 540, 547,. 554, 561, 568, 575 , 582C, 589C , 596C, 603C, 610C 617C, 624C , 631C 666, 673, 680, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 3891 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 109 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92360 T 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 VENTRAL-ABDOMINAL-LEFT WEEK 61 M 101 EYES PALE EYES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 80 VENTRAL-ABDOMINAL-LEFT WEEK 73 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 638C , 645C , 652C, 659C, 666C, 673C , 680C, 687C, 694C, 701C , 708C 715C , 722C , 729-730C 421, 428, 435,. 442, 449, 456, 505C , 512C , 519C, 526C, 533C, 582C , 589C , 596C, 603C, 610C, 659C , 666C , 673C, 680C, 687C, 729- 730C 463C , 470C, 540C , 547C, 617C , 624C, 694C , 701C, 477C, 554C, 631C, 708C, 484C, 561C, 638C, 715C, 491C , 498C 568C , 575C 645C , 652C 722C 701- 702 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498 554, 561, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C , 645C , 652C , 659C , 666C , 673C, 680C , 687C , 694C 701-7 02C 505C, 512C , 519C , 526C , 533C , 540C , 547C, 554C , 561C , 568C , 575C 582C, 589C , 596C , 603C , 610C , 617C , 624C, 631C , 638C , 645C , 652C 659C, 666C , 673C , 680C , 687C , 694C , 701-702C 3892 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 110 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92362 T 105 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB MOUTH NORMAL NO REMARKABLE OBSERVATIONS C92363 M MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 69 WEEK 102 83 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-LEFT NORMAL NO REMARKABLE OBSERVATIONS 701, 708 554, 561, 568, 575 1, 8,, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 519, 526, 533,. 540 , 547, 582, 589, 596 , 603, 610, 617, 624 , 631 638, 645, 652, 659 , 666, 673, 680, 687 , 694 477, 484, 491, 498 , 505, 512 708C, 715C , 722C, 729-730C 260, 267, 274, 281 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470, 477 344 1, 8,, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 288, 295, 302, 309 , 316 3893 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 111 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92363 M 83 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 76 WART LIKE LESION(S) MOUTH WEEK 54 C92364 M 63 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> CIRCLING RIGHT EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE ROUGH HAIR COAT DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 323, 330, 337, 484, 491, 498, 505, 512, 519 526C, 533C, 540C, 547C, 554C, 561C, 568C, 575C 372, 379, 386 428, 431, 435, 438 400, 407, 414, 421, 428, 431, 435, 438 428, 431, 435, 438 438 414, 421 421, 428, 431, 435, 438 428, 431, 435, 438 3894 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 112 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92364 M 63 NORMAL NO REMARKABLE OBSERVATIONS C92365 3 MASS(ES) AXILLARY REGION-RIGHT WEEK 57 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C92366 1 C92367 T 4 NORMAL NO REMARKABLE OBSERVATIONS 105 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372, 379 386 393, 400, 407, 414 , 421 176, 183, 190, 197 , 204, 211, 218, 225 , 232, 239, 246, 253, 260 267, 274, 281, 288 , 295, 302, 309, 316 , 323, 330, 337 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 344, 351, 358, 365 367 1, 8, 15, 22-23 1, 8 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 3895 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 113 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92368 M 102 APPEARANCE HEAD TILT LEFT MALOCCLUSION EXCRETION FEW FECES NO FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 91 VENTRAL-ABDOMINAL WEEK 100 701, 302, 393, 484, 575, 666, 706, 309, 400, 491, 582, 673, 708, 712 316,. 323, 407,. 414, 498,. 505, 589,. 596, 680,. 687, 330, 421, 512, 603, 694, 337, 428, 519, 610, 701, 344, 435, 526, 617, 706, 351, 442, 533, 624, 708, 358, 449, 540, 631, 712 365, 456, 547, 638, 372 , 379 , 386 463 , 470 , 477 554 , 561 , 568 645 , 652 , 659 706, 708 712 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183 , 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267, 274 , 281 631, 638C, 645C, 652C, 659C, 666C, 673C, 680C , 687C, 694C, 701C 706C, 708C , 712C 694C, 701C , 706C, 708C, 712C 3896 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 114 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92369 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 102 VENTRAL-ABDOMINAL-LEFT WEEK 51 C92370 M VENTRAL-ABDOMINAL-RIGHT WEEK 51 100 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 708C , 715C, 722C, 729-730C 351C , 358C, 428C , 435C, 505C , 512C, 582C , 589C, 659C , 666C, 729- 730C 365C, 442C, 519C, 596C, 673C, 372C, 44 9C, 526C, 603C, 680C, 379C, 456C, 533C, 610C, 687C, 386C , 393C , 400C, 463C , 470C , 477C, 540C , 547C , 554C, 617C , 624C , 631C, 694C , 701C , 708C, 407C, 484C, 561C, 638C, 715C, 414C , 421C 491C , 498C 568C , 575C 645C , 652C 722C 351C , 358C, 365C, 372C, 379C, 386C , 393C , 400C, 407C 554, 561, 568 687-688, 694, 697 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 3897 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 115 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) M 100 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 80 VENTRAL-ABDOMINAL-LEFT WEEK 100 VENTRAL-THORACIC WEEK 80 C92371 D 89 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687-688C, 694C 697C 697C 554C, 561C, 568C, 575C, 582C, 58 9C, 596C, 603C, 610C, 617C, 624C 631C, 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687-688C, 694C 697C 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 554 , 561, 568, 575, 582 , 589 596, 603, 610, 617, 619 526, 533, 540, 547 3898 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 116 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92372 3 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C92373 1 C92374 T 4 105 NORMAL NO REMARKABLE OBSERVATIONS APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 351, 358 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 365, 367 1, 8,r 15, 22- 23 106, 197, 288, 379, 470, 561, 652, 113, 204, 295, 386, 477, 568, 659, 120 , 127 , 134, 211 , 218 , 225, 302 , 309 , 316, 393 , 400 , 407, 484 , 491 , 498, 575 , 582 , 589, 666 , 673 , 680, 141, 232, 323, 414, 505, 596, 687, 148, 239, 330, 421, 512, 603, 694, 155 , 162, 246 , 253, 337 , 344, 428 , 435, 519 , 526, 610 , 617, 701 , 708, 169, 260, 351, 442, 533, 624, 715, 176, 267, 358, 449, 540, 631, 722, 183, 190 274, 281 365, 372 456, 463 547, 554 638, 645 729- 730 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99 365, 372C, 379C, 386C, 393C, 400C 3899 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 117 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92375 M 87 APPEARANCE HUNCHED DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 601, 603, 605 601, 603 605 596 601, 603 605 601, 603 605 589, 596 601, 603, 605 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582 3900 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 118 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92376 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92377 D MASS(ES) VENTRAL-CERVICAL WEEK 46 57 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 31 1, 8,r 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309 316C , 323C , 330C, 337C, 344C, 351C , 358C, 365C, 367C 64, 71, 78 , 85 , 92, 99, 106, 113, 120, 127, 134, 141, 148, 155 162, 169, 176, 183, 190, 197, 204, 211, 218, 225, 232, 239, 246 253, 260, 267, 274, 281, 288, 295, 302, 309, 316, 323, 330, 337 344, 351, 358, 365, 372, 379, 386, 393 1, 8 , 15, 22, 29, 36, 43 , 50, 57 211, 218, 225, 232, 239C , 246C, 253C, 260C, 267C, 274C , 281C 288C , 295C , 302C, 309C, 316C, 323C , 330C, 337C, 344C, 351C, 358C 365C, 372C, 379C, 386C, 393C 3901 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 119 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92378 M 73 APPEARANCE MALOCCLUSION THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 190 505, 508 508 508 505, 508 508 505, 508 508 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 197 , 204 211, 218, 225, 232 , 239, 246, 253, 260 , 267, 274, 281, 288 , 295 316, 323, 330, 337 , 344, 351, 358, 365 , 372, 379, 386, 393 , 400 407, 414, 421, 428 , 435, 442, 449, 456 , 463, 470, 477, 484 , 491 498 3902 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 120 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92378 M 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 C92379 D 72 APPEARANCE THIN EXCRETION FEW FECES NO FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 VENTRAL-CERVICAL WEEK 65 VENTRAL-THORACIC WEEK 70 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 302, 309 491, 498 491, 498 449 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190, 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309,. 316, 323, 330 337, 344, 351,. 358, 365, 372, 379, 386, 393, 400 , 407, 414C 421C, 428C , 435C, 442C, 44 9C, 456C , 463C, 470C, 477C, 484C, 491C 498C 449C 484C, 491C, 498C 3903 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 121 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92380 M 81 APPEARANCE MALOCCLUSION THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 45 AXILLARY REGION-RIGHT WEEK 60 VENTRAL-ABDOMINAL-LEFT WEEK 50 106, 113, 120,. 127, 197, 204, 211,. 218, 288, 295, 302,. 309, 379, 386, 393, 400, 470, 477, 484, 491, 559- 561 554, 559-561 134, 225, 316, 407, 498, 141, 232, 323, 414, 505, 148, 239, 330, 421, 512, 155, 246, 337, 428, 519, 162, 253, 344, 435, 526, 169 , 176 , 183, 260 , 267 , 274, 351 , 358 , 365, 442 , 449 , 456, 533 , 540 , 547, 190 281 372 463 554 554, 560-561 559 554, 559-561 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85 , 92, 99 309, 316, 323, 330, 337, 344C, 351C, 358C, 365C, 372C , 379C 386C , 393C, 400C, 407C, 414C, 421C , 428C, 435C, 442C, 44 9C, 456C 463C , 470C, 477C 414, 421, 428,. 435, 442, 449, 456, 463C, 470C, 477C, 484C, 491C 498C , 505C, 512C, 519C, 526C, 533C , 540C, 547C, 554C, 559-561C 344C , 351C, 358C, 365C, 372C, 379C , 386C, 393C, 400C, 407C 3904 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 122 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92381 M 69 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> SENSITIVE TO TOUCH DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS 477, 479-480 480 477 477, 479-480 463, 470, 477, 479 480 477, 479 -480 463, 470 , 477, 479-480 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267 295, 302, 309, 351 , 358, 365, 372, 379 , 386, 393 421, 428, 435, 442 , 449, 456 92, 99, 106 183, 190, 197 274, 281, 288 400, 407, 414 3905 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 123 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92381 M 69 MASS(ES) VENTRAL-CERVICAL WEEK 46 C92382 M 57 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL-RIGHT SKIN & PELAGE BROWN HAIR COAT GENERALIZED NORMAL NO REMARKABLEOBSERVATIONS DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 316, 323, 330, 337, 344C 386, 393 393 393 386, 393 386, 393 393 1, 8, 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 3906 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 124 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92383 T 105 APPEARANCE MALOCCLUSION SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL NORMAL NO REMARKABLE OBSERVATIONS C92384 M MASS(ES) AXILLARY REGION-LEFT WEEK 96 88 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES 29, 36 701, 708, 715,. 722, 729- 730 1, 8,, 15, 127, 134, 218, 225, 309, 316, 400, 407, 491, 498, 582, 589, 22, 43, 50, 57 , 64, 141,. 148, 155, 162, 232, 239, 246, 253, 323, 330, 337, 344, 414, 421, 428, 435, 505,. 512, 519, 526, 596, 603, 610, 617, 71, 78, 85 , 92, 99 , 106, 169, 176, 183, 190 , 197, 260, 267, 274, 281 , 288, 351, 358, 365, 372 , 379, 442, 449, 456, 463 , 470, 533, 540, 547, 554 , 561, 624, 631, 638, 645 , 652, 113 , 120 204 , 211 295 , 302 386 , 393 477 , 484 568 , 575 659 666C, 673C , 680C, 687C, 694C, 701C , 708C , 715C, 722C, 729- 730C 613-614 613-614 610, 613 614 3907 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 125 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92384 M 88 EYES RED DISCHARGE EYES SKIN & PELAGE BROWN HAIR COAT VENTRAL-ABDOMINAL PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS C92385 M 96 APPEARANCE HEAD TILT LEFT THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 610, 613-614 613- 614 614 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 22, 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 658-659, 666 666 589, 593, 596, 603 658-659, 666 3908 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 126 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92385 M 96 EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS C92386 T 105 APPEARANCE THIN EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB AXILLARY REGION-LEFT VENTRAL-CERVICAL-LEFT DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 596, 603, 610, 617 , 624, 631, 638, 645 , 652 589, 593, 658- 659, 666 593, 596, 603, 610 , 617 582, 589, 593, 596 , 603, 610, 617, 624 , 631 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575 701, 708, 715, 722, 729-730 638, 645, 652, 659, 666, 673, 680, 687 582, 589 701, 708, 715, 722 3909 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 127 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92386 T 105 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 76 INGUINAL REGION-LEFT WEEK 101 VENTRAL-ABDOMINAL WEEK 80 VENTRAL-CERVICAL-LEFT WEEK 95 WART LIKE LESION(S) EAR-LEFT WEEK 92 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36 , 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155 , 162 , 169, 176, 183 , 190 , 197 204, 211, 218, 225, 232, 239, 246 , 253 , 260, 267, 274 , 281 , 288 295, 302, 309,. 316, 323, 330, 337 , 344 , 351, 358, 365 , 372 , 379 386, 393, 400, 407, 414, 421, 428 , 435 , 442, 449, 456 , 463 , 470 477, 484, 491, 498, 505, 512, 519 526, 533, 540,. 547C, 554C , 561C, 568C, 575C, 582C, 58 9C, 596C 603C , 610C, 617C, 624C, 631C , 638C, 645C , 652C 701C , 708C, 715C, 722C, 729- 730C 554C , 561C, 568C, 575C, 582C , 589C, 596C , 603C, 610C, 617C, 624C 631C , 638C, 645C, 652C, 659C , 666C, 673C , 680C, 687C, 694C, 701C 708C , 715C, 722C, 729-730C 659, 666, 673, 680 , 687,, 694 , 701, 708, 715C, 722C, 729-730C 638, 645, 652, 659 , 666, 673 , 680, 687, 694, 701, 708,, 715, 722 729- 730 3910 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 128 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92387 M 74 APPEARANCE THIN EYES SQUINTED PERI-ORBITAL SKIN & PELAGE RED HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 59 VENTRAL-ABDOMINAL-LEFT WEEK 51 512-513 513 513 513 1, 8,, 15, 22, 29, 36, 43, 50 , 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 , 155, 162, 169, 176, 183 , 190, 197 204, 211, 218, 225, 232, 239 , 246, 253, 260, 267, 274 , 281, 288 295, 302, 309, 316, 323, 330 , 337, 344, 365, 372, 379 , 386, 393 400 407, 414C, 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C 484C, 491C , 498C, 505C, 512- 513C 351, 358 3911 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 129 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92388 3 53 SKIN & PELAGE SORE/SCAB HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS C92389 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92390 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92391 D 93 EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT 309, 316 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 323, 330, 337, 344, 351, 358 , 365, 367 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365, 367 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,, 316, 323, 330, 337, 344 , 351, 358, 365, 367 601, 603 470, 477, 484, 491, 498, 505, 512, 519 , 526, 533, 540, 547 , 554 561, 568, 575,, 582, 589, 596, 601, 603 , 610, 617, 624, 631 , 638 t6Z4A 5C, 6tz 4a 7n 3912 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 130 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92391 D 93 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 61 C92 392 M INGUINAL REGION-RIGHT WEEK 84 VENTRAL-ABDOMINAL-LEFT WEEK 49 VENTRAL-ABDOMINAL-RIGHT WEEK 84 64 APPEARANCE HEAD TILT LEFT DISCHARGE NASAL RED IN COLOR EYES DISCOLORED/LIGHT RED EYES DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 12 7, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330 421C, 428C , 435C, 442C, 44 9C, 456C, 463C , 470C, 477C, 484C, 491C 498C, 505C , 512C, 519C, 526C, 533C, 540C , 547C, 554C, 561C, 568C 575C, 582C , 589C, 596C, 601C, 603C, 610C , 617C, 624C, 631C, 638C 645C, 647C 582C, 589C , 596C, 601C, 603C, 610C, 617C , 624C, 631C, 638C, 645C 647C 337, 344, 351, 358 , 365,, 372, 379, 386, 393, 400 , 407, 414 582C, 589C , 596C, 601C, 603C, 610C, 617C , 624C, 631C, 638C, 645C 647C 435, 442 365, 435, 442 435, 442 3913 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 131 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92392 M 64 EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS C92393 M 94 APPEARANCE MALOCCLUSION THIN SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 60 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 365, 372, 379, 386 , 393, 400, 442 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 407, 414, 421 428 653 638, 645, 652- 653 50, 57, 64 , 71 , 239, 246,, 253, 260 , 267, 274, 302, 309, 316, 344 351, 358, 365, 372, 379, 386, 393, 400, 407, 414, 421, 428, 435 442, 449, 456, 463, 470, 477, 484, 491, 498 1, 8 , 15, 22, 29, 36, 43,, 78, 85, 92, 99, 106, 113 , 120 , 127 134, 141, 148, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218 225, 232, 281, 288, 295, 323, 330, 337 414C, 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C, 638C 64 5C, 652-653C 3914 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 132 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 (CONTINUED FROM PREVIOUS PAGE) C92393 M 94 MASS(ES) INGUINAL REGION-LEFT WEEK 92 VENTRAL-ABDOMINAL-LEFT WEEK 73 C92394 M 85 APPEARANCE THIN DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-CERVICAL WEEK 36 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 638C, 64 5C, 652-653C 505C , 512C , 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C 582C , 58 9C , 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C 652- 653C 568, 570, 575, 582 , 589 568, 570, 575, 582, 589 568, 570, 575 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218,, 225, 232, 239 246C, 323C, 400C, 477C, 554C, 253C, 330C, 407C, 484C, 561C, 260C, 337C, 414C, 491C, 568C, 267C, 344C, 421C, 498C, 570C, 274C, 351C, 428C, 505C, 575C, 281C, 358C, 435C, 512C, 582C, 288C, 365C, 442C, 519C, 589C 295C, 372C, 44 9C, 526C, 302C, 379C, 456C, 533C, 3 0 9C, 386C, 463C, 54 0C, 316C 393C 470C 547C 3915 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 133 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F8 DOSE: 0 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS :LISTED AT END OF OBSERVATIONS C92395 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92396 2 C92397 2 C92398 2 C92399 2 C92400 2 14 NORMAL NO REMARKABLE OBSERVATIONS 14 EYES DISPLACED PUPIL EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 14 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS 1, 8 , 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148 , 155 , 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239 , 246 , 253 , 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330 , 337 , 344 , 351, 358, 365, 367 1, 8 , 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78, 85, 92-93 29, 92-93 1, 8 , 15, 22, 36, 43, 50, 57 , 64, 71, 78, 85 1, 8 , 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78, 85, 92-93 1, 8 , 15, 22, 29, 36, 43, 50 , 57, 64, 71, 78, 85, 92-93 78, 85, 92 -93 1, 8 , 15, 22, 29, 36, 43, 50 , 57, 64, 71 3916 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92331 F8 288 42 295 43 302 44 TO 407 59 414 60 TO 484 70 491 71 TO 512 74 519 75 526 76 TO 540 78 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 10 X 10MM 10 X 10MM 20 X 10MM 20 X 15MM 20 X 20MM 30 X 30MM 35 X 35MM 50 X O55MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 134 3917 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 80 MASS(ES) 82 AXILLARY REGION-RIGHT WEEK 42 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 84 MASS(ES) 91 AXILLARY REGION-RIGHT WEEK 42 92 MASS(ES) 92 AXILLARY REGION-RIGHT WEEK 42 59 MASS(ES) AXILLARY REGION-LEFT WEEK 59 60 MASS(ES) AXILLARY REGION-LEFT WEEK 59 61 MASS(ES) 64 AXILLARY REGION-LEFT WEEK 59 65 MASS(ES) 69 AXILLARY REGION-LEFT WEEK 59 50 X 55MM 25 X 55MM 60 X 55MM 60 X 40MM 10 X 10MM 20 X 15MM 25 X 25MM 35 X 35MM 3918 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92336 C92337 F8 484 70 TO 512 74 519 75 TO 540 78 547 79 TO 570 82 519 75 526 76 TO 540 78 547 79 TO 570 82 F8 274 40 344 50 TO 470 68 MASS(ES) AXILLARY REGION-LEFT WEEK 59 MASS(ES) AXILLARY REGION-LEFT WEEK 59 MASS(ES) AXILLARY REGION-LEFT WEEK 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 75 DISCHARGE OTHER MASS(ES) VENTRAL-ABDOMINAL WEEK 50 45 X 45MM 55 X 55MM 60 X 70MM 15 X 15MM 20 X 20MM 30 X 35MM MUCOID DISCHARGE FROM RECTUM 20 X 20MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 136 3919 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 69 MASS(ES) 72 VENTRAL-ABDOMINAL WEEK 50 73 MASS(ES) 74 VENTRAL-ABDOMINAL WEEK 50 75 MASS(ES) 78 VENTRAL-ABDOMINAL WEEK 50 79 MASS(ES) 82 VENTRAL-ABDOMINAL WEEK 50 73 MASS(ES) 74 VENTRAL-ABDOMINAL-LEFT WEEK 73 38 MASS(ES) 40 SHOULDER-LEFT WEEK 38 41 MASS(ES) 49 SHOULDER-LEFT WEEK 38 50 MASS(ES) 52 SHOULDER-LEFT WEEK 38 25 X 30MM 35 X 30MM 35 X 20MM 35 X 30MM 10 X 10MM 20 X 15MM 20 X 20MM 20 X 10MM 3920 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 68 MASS(ES) 69 VENTRAL-ABDOMINAL-RIGHT WEEK 66 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 71 MASS(ES) 74 VENTRAL-ABDOMINAL-RIGHT WEEK 66 75 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 76 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 66 77 MASS(ES) 78 VENTRAL-ABDOMINAL-RIGHT WEEK 66 79 MASS(ES) 83 VENTRAL-ABDOMINAL-RIGHT WEEK 66 84 MASS(ES) 87 VENTRAL-ABDOMINAL-RIGHT WEEK 66 15 X 15MM 20 X 20MM 25 X 25MM 40 X 50MM 45 X 60MM 55 X 35MM 70 X 50MM 75 X 60MM 3921 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 88 MASS(ES) 94 VENTRAL-ABDOMINAL-RIGHT WEEK 66 79 MASS(ES) 79 INGUINAL REGION-LEFT WEEK 79 74 MASS(ES) 78 VENTRAL-ABDOMINAL WEEK 74 79 MASS(ES) 79 VENTRAL-ABDOMINAL WEEK 74 50 MASS(ES) 52 VENTRAL-ABDOMINAL-LEFT WEEK 44 53 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 54 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 55 MASS(ES) 56 VENTRAL-ABDOMINAL-LEFT WEEK 44 90 X 80MM 30 X 35MM 20 X 20MM 35 X 30MM 10 X 10MM 20 X 15MM 25 X 20MM 25 X 30MM 3922 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 57 MASS(ES) 59 VENTRAL-ABDOMINAL-LEFT WEEK 44 60 MASS(ES) 64 VENTRAL-ABDOMINAL-LEFT WEEK 44 65 MASS(ES) 69 VENTRAL-ABDOMINAL-LEFT WEEK 44 70 MASS(ES) 71 VENTRAL-ABDOMINAL-LEFT WEEK 44 72 MASS(ES) 75 VENTRAL-ABDOMINAL-LEFT WEEK 44 76 MASS(ES) 78 VENTRAL-ABDOMINAL-LEFT WEEK 44 79 MASS(ES) 79 VENTRAL-ABDOMINAL-LEFT WEEK 44 74 MASS(ES) 78 VENTRAL-THORACIC-LEFT WEEK 74 40 X 30MM 50 X 50MM 50 X 65MM 70 X 70MM 80 X 70MM 85 X 80MM 100 X 85MM 20 X 20MM 3923 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92340 C92341 C92343 F8 547 79 TO 548 79 F8 365 53 TO 367 53 F8 127 19 134 20 TO 162 24 169 25 TO 176 26 183 27 TO 218 32 225 33 TO 253 37 260 38 TO 281 41 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 MASS(ES) SHOULDER-RIGHT WEEK 19 MASS(ES) SHOULDER-RIGHT WEEK 19 MASS(ES) SHOULDER-RIGHT WEEK 19 MASS(ES) SHOULDER-RIGHT WEEK 19 MASS(ES) SHOULDER-RIGHT WEEK 19 MASS(ES) SHOULDER-RIGHT WEEK 19 35 X 20MM 10 X 10MM 10 X 15MM 15 X 20MM 30 X 30MM 30 X 40MM 40 X 55MM 50 X 40MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 141 3924 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92343 C92348 F8 288 42 TO 302 44 MASS(ES) SHOULDER-RIGHT WEEK 19 309 45 MASS(ES) SHOULDER-RIGHT WEEK 19 316 46 TO 330 48 MASS(ES) SHOULDER-RIGHT WEEK 19 337 49 TO 400 58 MASS(ES) SHOULDER-RIGHT WEEK 19 F8 701 101 MASS(ES) TO 708 102 AXILLARY REGION-RIGHT WEEK 101 715 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 722 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 729 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 65 X 50MM 60 X 70MM 65 X 50MM 65 X 60MM 20 X 20MM 40 X 35MM 40 X 35MM 50 X 45MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 142 3925 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92348 F8 730 105 MASS(ES) AXILLARY REGION-RIGHT WEEK 101 694 100 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 701 101 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 708 102 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 715 103 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 722 104 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 729 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 730 105 MASS(ES) VENTRAL-ABDOMINAL WEEK 100 50 X 45MM 40 X 45MM 45 X 40MM 45 X 35MM 60 X 40MM 60 X 40MM 60 X 40MM 60 X 40MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 143 3926 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92349 C92350 C92354 C92358 C92359 F8 344 50 TO 400 58 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 F8 656 94 DISCHARGE OTHER 656 94 DISCHARGE OTHER F8 610 88 MASS(ES) TO 724 104 AXILLARY REGION-RIGHT WEEK 83 F8 610 88 MASS(ES) TO 708 102 AXILLARY REGION-RIGHT WEEK 84 715 103 MASS(ES) TO 730 105 AXILLARY REGION-RIGHT WEEK 84 F8 638 92 TO 648 93 MASS(ES) AXILLARY REGION-LEFT WEEK 92 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 10 X 10MM RED ORAL RED ORAL 10 X 10MM 10 X 10MM 20 X 20MM 10 X 10MM 20 X 10MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 144 3927 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C92359 F8 554 80 TO 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 582 84 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 610 88 TO 648 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 79 554 80 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL WEEK 80 F8 582 84 TO 631 91 MASS(ES) AXILLARY REGION-LEFT WEEK 77 687 99 MASS(ES) TO 708 102 AXILLARY REGION-LEFT WEEK 96 715 103 MASS(ES) TO 722 104 AXILLARY REGION-LEFT WEEK 96 729 105 MASS(ES) TO 730 105 AXILLARY REGION-LEFT WEEK 96 10 X 10MM 25 X 25MM 30 X 30MM 10 X 10MM 10 X 10MM 30 X 20MM 55 X 50MM 60 X 60MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 145 3928 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C92360 F8 638 92 TO 645 93 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 652 94 TO 666 96 MASS(ES) AXILLARY REGION-RIGHT WEEK 92 673 97 MASS(ES) TO 708 102 AXILLARY REGION-RIGHT WEEK 92 715 103 MASS(ES) TO 730 105 AXILLARY REGION-RIGHT WEEK 92 463 67 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 505 73 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 519 75 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 547 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 10 X 10MM 20 X 20MM 30 X 25MM 45 X 45MM 25 X 25MM 35 X 35MM 35 X 50MM 50 X 50MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 146 3929 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C92360 F8 554 80 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 666 96 TO 687 99 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 694 100 MASS(ES) TO 708 102 VENTRAL-ABDOMINAL-LEFT WEEK 61 715 103 MASS(ES) TO 730 105 VENTRAL-ABDOMINAL-LEFT WEEK 61 F8 568 82 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 80 582 84 TO 603 87 MASS(ES) AXILLARY REGION-LEFT WEEK 80 610 88 MASS(ES) TO 702 101 AXILLARY REGION-LEFT WEEK 80 505 73 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 70 X 60MM 65 X 45MM 65 X 50MM 80 X 65MM 20 X 20MM 25 X 30MM 40 X 35MM 10 X 10MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 147 3930 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92361 F8 526 76 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 547 79 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 561 81 TO 575 83 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 582 84 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 596 86 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 610 88 TO 659 95 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 666 96 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 673 97 MASS(ES) TO 694 100 VENTRAL-ABDOMINAL-LEFT WEEK 73 25 X 25MM 30 X 45MM 50 X 50MM 60 X 65MM 70 X 65MM 85 X 70MM 90 X 65MM 90 X 85MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 148 3931 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 101 MASS(ES) 101 VENTRAL-ABDOMINAL-LEFT WEEK 73 102 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 102 103 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 102 104 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 102 105 MASS(ES) 105 VENTRAL-ABDOMINAL-LEFT WEEK 102 76 MASS(ES) 78 AXILLARY REGION-LEFT WEEK 76 79 MASS(ES) AXILLARY REGION-LEFT WEEK 76 80 MASS(ES) 81 AXILLARY REGION-LEFT WEEK 76 110 X 100MM 10 X 10MM 30 X 20MM 20 X 10MM 30 X 30MM 20 X 20MM 50 X 55MM 60 X 55MM 3932 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92363 C92368 C92369 F8 568 82 MASS(ES) AXILLARY REGION-LEFT WEEK 76 575 83 TO 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 76 575 83 MASS(ES) AXILLARY REGION-LEFT WEEK 76 F8 638 92 MASS(ES) TO 706 101 AXILLARY REGION-RIGHT WEEK 91 708 102 MASS(ES) TO 712 102 AXILLARY REGION-RIGHT WEEK 91 694 100 MASS(ES) TO 712 102 VENTRAL-ABDOMINAL WEEK 100 F8 708 102 MASS(ES) AXILLARY REGION-LEFT WEEK 102 715 103 MASS(ES) AXILLARY REGION-LEFT WEEK 102 80 X 60MM 80 X 80MM 80 X 60MM 20 X 25MM 35 X 30MM 40 X 30MM 10 X 10MM 25 X 20MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 150 3933 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92369 F8 722 104 MASS(ES) AXILLARY REGION-LEFT WEEK 102 729 105 MASS(ES) AXILLARY REGION-LEFT WEEK 102 730 105 MASS(ES) AXILLARY REGION-LEFT WEEK 102 351 51 TO 414 60 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 421 61 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 449 65 TO 477 69 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 484 70 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 505 73 TO 512 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 25 X 20MM 25 X 20MM 25 X 20 MM 10 X 10MM 20 X 20MM 30 X 30MM 35 X 35MM 45 X 40MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 151 3934 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92369 F8 519 75 TO 526 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 533 77 TO 540 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 547 79 TO 589 85 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 596 86 TO 603 87 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 51 610 88 MASS(ES) TO 708 102 VENTRAL-ABDOMINAL-LEFT WEEK 51 715 103 MASS(ES) TO 722 104 VENTRAL-ABDOMINAL-LEFT WEEK 51 729 105 MASS(ES) TO 730 105 VENTRAL-ABDOMINAL-LEFT WEEK 51 351 51 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 51 50 X 55MM 51 X 40MM 65 X 60MM 70 X 60MM 85 X 70MM 100 X 110MM 115 X 110MM 15 X 15MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 152 3935 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C92370 F8 554 80 TO 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 568 82 TO 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 582 84 TO 589 85 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 596 86 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 610 88 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 80 638 92 MASS(ES) TO 697 100 AXILLARY REGION-RIGHT WEEK 80 697 100 MASS(ES) TO 697 100 VENTRAL-ABDOMINAL-LEFT WEEK 100 554 80 TO 561 81 MASS(ES) VENTRAL-THORACIC WEEK 80 10 X 10MM 20 X 20MM 30 X 30MM 30 X 40MM 50 X 50MM 55 X 55MM 15 X 15MM 10 X 10MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 153 3936 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92370 C92374 C92376 C92377 C92379 F8 568 82 TO 603 87 MASS(ES) VENTRAL-THORACIC WEEK 80 610 88 MASS(ES) TO 697 100 VENTRAL-THORACIC WEEK 80 F8 372 54 TO 400 58 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 53 F8 316 46 TO 367 53 MASS(ES) VENTRAL-CERVICAL WEEK 46 F8 239 35 TO 309 45 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 31 316 46 TO 393 57 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 31 F8 414 60 TO 421 61 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 428 62 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 49 30 X 30MM 45 X 35MM 10 X 10MM 30 X 30MM 15 X 15MM 20 X 20MM 15 X 10MM 20 X 15MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 154 3937 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 65 MASS(ES) VENTRAL-CERVICAL WEEK 65 70 MASS(ES) 72 VENTRAL-THORACIC WEEK 70 50 MASS(ES) 55 AXILLARY REGION-LEFT WEEK 45 56 MASS(ES) 59 AXILLARY REGION-LEFT WEEK 45 60 MASS(ES) 69 AXILLARY REGION-LEFT WEEK 45 67 MASS(ES) 72 AXILLARY REGION-RIGHT WEEK 60 73 MASS(ES) 75 AXILLARY REGION-RIGHT WEEK 60 76 MASS(ES) 78 AXILLARY REGION-RIGHT WEEK 60 10 X 10MM 10 X 10MM 10 X 10MM 15 X 15MM 20 X 20MM 20 X 20MM 40 X 30MM 45 X 45MM 3938 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92380 C92381 C92383 C92386 F8 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 554 80 TO 561 81 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 344 50 TO 358 52 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 365 53 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 50 F8 344 50 MASS(ES) VENTRAL-CERVICAL WEEK 46 F8 666 96 MASS(ES) TO 708 102 AXILLARY REGION-LEFT WEEK 96 715 103 MASS(ES) TO 730 105 AXILLARY REGION-LEFT WEEK 96 F8 547 79 MASS(ES) AXILLARY REGION-LEFT WEEK 76 50 X 50MM 45 X 45MM 10 X 10MM 20 X 30MM 10 X 10MM 20 X 20MM 40 X 40MM 10 X 20MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 156 3939 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 80 MASS(ES) 94 AXILLARY REGION-LEFT WEEK 76 101 MASS(ES) 102 INGUINAL REGION-LEFT WEEK 101 103 MASS(ES) 105 INGUINAL REGION-LEFT WEEK 101 80 MASS(ES) 83 VENTRAL-ABDOMINAL WEEK 80 84 MASS(ES) VENTRAL-ABDOMINAL WEEK 80 85 MASS(ES) 91 VENTRAL-ABDOMINAL WEEK 80 92 MASS(ES) 104 VENTRAL-ABDOMINAL WEEK 80 105 MASS(ES) 105 VENTRAL-ABDOMINAL WEEK 80 15 X 10MM 10 X 10MM 30 X 30MM 10 X 10MM 30 X 25MM 30 X 30MM 30 X 40MM 40 X 40MM 3940 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92386 C92387 C92391 F8 715 103 MASS(ES) TO 730 105 VENTRAL-CERVICAL-LEFT WEEK 95 F8 414 60 TO 435 63 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 442 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 449 65 TO 498 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 505 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 512 74 TO 513 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 F8 421 61 MASS(ES) AXILLARY REGION-LEFT WEEK 61 428 62 TO 540 78 MASS(ES) AXILLARY REGION-LEFT WEEK 61 10 X 10MM 10 X 10MM 15 X 15MM 30 X 30MM 45 X 40MM 45 X 30MM 10 X 10MM 15 X 15MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 158 3941 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92391 C92393 F8 547 79 TO 647 93 582 84 TO 647 93 582 84 TO 631 91 638 92 TO 647 93 F8 414 60 TO 421 61 428 62 TO 442 64 449 65 TO 477 69 484 70 TO 519 75 MASS(ES) AXILLARY REGION-LEFT WEEK 61 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 84 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 20 X 30MM 10 X 10MM 10 X 10MM 20 X 15MM 20 X 20MM 30 X 30MM 40 X 40MM 55 X 50MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 159 3942 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT COMMENT C92393 F8 526 76 TO 540 78 547 79 TO 589 85 596 86 TO 603 87 610 88 TO 652 94 653 94 TO 653 94 638 92 TO 653 94 505 73 TO 554 80 561 81 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) AXILLARY REGION-RIGHT WEEK 60 MASS(ES) INGUINAL REGION-LEFT WEEK 92 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 73 60 X 60MM 60 X 70MM 70 X 80MM 95 X 80MM 110 X 90MM 15 X 15MM 10 X 10MM 15 X 15MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 160 3943 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 92 MASS(ES) 94 VENTRAL-ABDOMINAL-LEFT WEEK 73 36 MASS(ES) 39 VENTRAL-CERVICAL WEEK 36 40 MASS(ES) 45 VENTRAL-CERVICAL WEEK 36 46 MASS(ES) 53 VENTRAL-CERVICAL WEEK 36 54 MASS(ES) 64 VENTRAL-CERVICAL WEEK 36 65 MASS(ES) 72 VENTRAL-CERVICAL WEEK 36 73 MASS(ES) 75 VENTRAL-CERVICAL WEEK 36 76 MASS(ES) 78 VENTRAL-CERVICAL WEEK 36 20 X 25MM 10 X 10MM 15 X 10MM 20 X 25MM 25 X 25MM 35 X 35MM 45 X 45MM 55 X 50MM 3944 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92394 F8 547 79 TO 589 85 MASS(ES) VENTRAL-CERVICAL WEEK 36 75 X 50MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 162 3945 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 163 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92401 M 92 APPEARANCE HEAD TILT LEFT MALOCCLUSION THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES NO FECES NONFORMED FECES EYES RED DISCHARGE EYE-LEFT EYES SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS 617, 624, 631, 633-634, 638, 641 638, 641 633-634, 638, 641 634, 638, 641 641 617, 624, 631, 633 633-634 547 634 641 638, 641 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 3946 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 164 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92401 M 92 NORMAL NO REMARKABLE OBSERVATIONS C92402 1 C92403 D 4 NORMAL NO REMARKABLE OBSERVATIONS 51 DISCHARGE GENITAL RED IN COLOR EYES RED DISCHARGE EYE-RIGHT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 35 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 554, 561 , 568 575, 582, 589, 596, 603, 610 1, 8,, 15, 22-23 260 155, 162 1, 8,, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78,, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 169, 176, 183, 190, 197, 204 , 211 218, 225, 232 239C, 246C , 253C, 260C, 267C, 274C , 281C, 288C, 295C, 302C , 309C 316C, 323C , 330C, 337C, 344C, 351C , 356C 3947 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 165 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92404 M 81 EYES SQUINTED PERI-ORBITAL SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL-LEFT YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 VENTRAL-ABDOMINAL-RIGHT WEEK 79 VENTRAL-CERVICAL-LEFT WEEK 70 566 554, 561, 566 566 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218,. 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400,. 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477 526C, 533C', 54 0C, 54 7C, 554C, 561C , 566C 547, 554, 561, 566 484, 491, 498, 505C, 512C, 519C, 526C, 533C, 54 0C, 547C, 554C 561C, 566C 3948 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 166 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92405 M 98 BEHAVIOR HYPOACTIVE <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES NONFORMED FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS 686 686 666, 668 673 664- 666 668, 673 582, 589 668, 673 680, 686 668, 673 680, 686 1, 8 , 15 113, 120 204, 211 295, 302 386, 393 477, 484 631, 638 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505, 512, 519, 526 , 533, 540, 610, 617 , 624 645, 652 , 659 3949 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 167 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92405 M 98 MASS(ES) AXILLARY REGION-LEFT WEEK 79 INGUINAL REGION-LEFT WEEK 84 C92406 3 53 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C92407 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92408 M 104 EYES PALE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 547, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 582C, 589C, 596C, 603C 176, 183, 190, 197 , 204, 211, 218, 225 , 232 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 239, 246, 253, 260 267, 274, 281, 288 , 295, 302, 309, 316 , 323, 330, 337, 344, 351 358, 365, 367 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 367 722 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 22, 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 400, 407, 414, 421, 491, 498 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 3950 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 168 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92408 M 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 VENTRAL-ABDOMINAL-RIGHT WEEK 92 C92409 M 99 APPEARANCE HEAD TILT RIGHT BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C 638C, 64 5C, 652C, 659C, 666C, 673C, 680C, 687C, 694C, 701C, 708C 715C, 722C 690-691 690-691 691 679-680 673, 679-680, 687, 690-691 673, 679-680, 687, 690-691 1, 8,, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 3951 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 169 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92409 M 99 NORMAL NO REMARKABLE OBSERVATIONS C92410 T MASS(ES) AXILLARY REGION-RIGHT WEEK 74 VENTRAL-ABDOMINAL-LEFT WEEK 66 WEEK 75 105 NORMAL NO REMARKABLE OBSERVATIONS ASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 103 VENTRAL-CERVICAL WEEK 88 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141 , 148 , 155, 162, 169, 176 , 183 , 190, 197 204, 211, 218, 225, 232 , 239 , 246, 253, 260, 267 , 274 , 281, 288 295, 302, 309, 316, 323 , 330 , 337, 344, 351, 358 , 365 , 372, 379 386, 393, 400, 407, 414 , 421 , 428, 435, 442, 449 , 491 , 498, 505 512, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C 58 9C, 596C , 603C, 610C, 617C , 624C, 631C , 638C, 645C, 652C, 659C 666C,. 673C , 679-680C, 687C, 690-691C 456, 463, 470, 477, 484 519C, 526C , 533C, 540C, 547C , 554C 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78 , 85 , 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582, 589, 596, 603 715, 722, 729- 730C 610C, 617C , 624C, 631C, 638C, 645C , 652C , 65 9C, 666C, 673C , 680C 687C, 694C , 701C, 708C, 715C, 722C , 729- 730C 3952 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 170 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92411 3 53 APPEARANCE MALOCCLUSION BEHAVIOR SENSITIVE TO TOUCH DISCHARGE NASAL RED IN COLOR EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE BLUE SKIN VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 148, 152, 155, 162, 169, 176, 183, 190, 197, 204, 211, 218, 225 232, 239, 246, 253, 260, 267, 274, 281, 288, 295, 302, 309, 316 323, 330, 337, 344, 351, 358, 365, 367 152 152 141 309, 316 1, 8,. 15, 22, 29, 36, 43, 50, 57, 64, 71,. 78, 85, 92, 99, 106 113, 120, 127, 134 302C,. 309C , 316C, 323C, 330C, 337C, 344, 351, 358, 365, 367 3953 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 171 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92412 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92413 M 102 APPEARANCE HEAD TILT RIGHT MALOCCLUSION BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES RESPIRATION LABORED SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666, 673, 680, 687, 694, 701, 708 , 715, 722, 729- 730 701, 281, 372, 463, 554, 645, 708, 288, 379, 470, 561, 652, 710 295, 386, 477, 568, 659, 302, 393, 484, 575, 666, 309, 400, 491, 582, 673, 316, 407, 498, 589, 680, 323, 414, 505, 596, 687, 330, 421, 512, 603, 694, 337, 428, 519, 610, 701, 344, 435, 526, 617, 708, 351, 442, 533, 624, 710 358, 449, 540, 631, 365 456 547 638 710 710 701, 708, 710 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 710 3954 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 172 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92413 M 102 NORMAL NO REMARKABLE OBSERVATIONS C92414 M MASS(ES) AXILLARY REGION-RIGHT WEEK 96 63 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 42 LIMB-FRONT-RIGHT WEEK 35 PAW-HIND-RIGHT WEEK 42 VENTRAL-ABDOMINAL-RIGHT WEEK 44 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274 666C, 673C, 680C, 687C, 694C, 701C, 708C, 710C 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232 288, 295 239, 246, 253, 260C, 267C, 274C, 281C 288C, 295C 302, 309C, 316C, 323C, 330C, 337C, 344C, 351C, 358C, 365C, 372C 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 3955 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 173 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92415 D 89 NORMAL NO REMARKABLE OBSERVATIONS C92416 T 105 SKIN & PELAGE RED HAIR COAT HEAD-CRANIAL SORE/SCAB TAIL TAIL-PROXIMAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 84 TAIL-PROXIMAL WEEK 75 1, 8,r 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 568, 575, 22, 29, 36 , 43,r 50, 127,. 134, 141, 148, 218, 225, 232, 239, 309,. 316, 323, 330, 400, 407, 414, 421, 491, 498, 505, 512, 582, 589, 596, 603, 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 519, 526 , 533, 540, 610, 617 , 619 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 547, 554 , 561 701, 708, 715, 722, 729-730 729-730 519, 526, 638, 645, 652, 701 1, 8,r 15, 113, 120, 204, 211, 295, 302, 386, 393, 477, 484, 22, 29, 36, 43,r 50, 127, 134 , 141, 148, 218, 225 , 232, 239, 309, 316 , 323, 330, 400, 407 , 414, 421, 491, 498 , 505, 512 57, 64, 71, 78, 85, 155, 162 , 169, 176, 246, 253 , 260, 267, 337, 344 , 351, 358, 428, 435 , 442, 449, 92, 99, 106 183, 190 , 197 274, 281 , 288 365, 372 , 379 456, 463 , 470 582C, 58 9C, 596C, 603C 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 589C 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 673C, 680C, 687C, 694C, 701C, 708C, 715C, 722C, 729-730C 3956 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 174 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92416 T 105 WART LIKE LESION(S) HEAD-MAXILLARY-RIGHT WEEK 79 C92417 M 94 APPEARANCE HEAD TILT LEFT RIGHT THIN BEHAVIOR ATAXIC <UNSPECIFIED> CIRCLING LEFT EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 547, 554, 561, 568, 575,. 582, 589,. 596, 603 , 610, 617, 624, 631 638, 645, 652, 659, 666,. 673, 680, 687,. 694 , 701, 708, 715, 722 729- 730 648, 652 653 622, 624, 631, 633- 634, 638, 645, 648, 652- 653 634 648, 652 596, 603, 610, 617, 622,. 624, 631,. 633, 648 , 652- 653 634, 638, 645 634 631, 633- 634, 638, 645, 648, 652-653 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183,. 190, 197 3957 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 175 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92417 M 94 NORMAL NO REMARKABLE OBSERVATIONS C92418 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92419 T 105 APPEARANCE MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 96 AXILLARY REGION-RIGHT WEEK 101 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 295, 386, 477, 568, 211, 302, 393, 484, 575, 218, 309, 400, 491, 582, 225, 316, 407, 498, 589 232, 323, 414, 505, 239, 330, 421, 512, 246, 337, 428, 519, 253, 344, 435, 526, 260, 351, 442, 533, 267, 358, 449, 540, 274, 365, 456, 547, 281, 372, 463, 554, 288 379 470 561 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 367 85, 92, 99, 10 6, 113 , 12 0, 127 , 134, 141 , 148 , 155 , 162 , 169 176, 183 , 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260 267, 274 , 281, 288, 295, 302, 309, 316, 323, 330, 337, 344, 351 358, 365 , 372, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442 449, 456 , 463, 470, 477, 484, 491, 498, 505, 512, 519, 526, 533 540, 547 , 554, 561, 568, 575, 582, 589, 596, 603, 610, 617, 624 631, 638 , 645, 652, 659, 666, 673, 680, 687, 694, 701, 708, 715 722, 729 -730 1, 8 , 15 , 22, 29, 36 , 43 , 50, 57, 64, 71 , 78 666, 673, 680, 687, 694,, 701, 708, 715, 722, 729-730 701C, 708C, 715C, 722C, 729-730C 3958 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 176 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92420 M 73 SKIN & PELAGE SORE/SCAB AXILLARY REGION-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92421 T MASS(ES) AXILLARY REGION-RIGHT WEEK 59 VENTRAL-ABDOMINAL WEEK 50 WEEK 56 105 APPEARANCE MALOCCLUSION SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-RIGHT 505 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 19 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 28 295, 302, 309,. 316, 323, 330, 337, 372 , 379 197 288 407C, 414C , 421C, 428C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C 484C, 491C , 498C, 505C 344, 351, 358, 365 386C, 393C , 400C 148, 239, 330, 421, 512, 603, 694, 155, 246, 337, 428, 519, 610, 701, 162, 253, 344, 435, 526, 617, 708, 169 , 176,. 183, 190, 260 , 267,. 274, 281, 351 , 358, 365, 372, 442 , 449,. 456, 463, 533 , 540, 547, 554, 624 , 631,. 638, 645, 715 , 722, 729- 730 197 , 204, 288 , 295, 379 , 386, 470 , 477, 561 , 568, 652 , 659, 211 , 218, 225, 302 , 309,. 316, 393 , 400, 407, 484 , 491, 498, 575 , 582, 589, 666 , 673, 680, 232 323 414 505 596 687 575, 582, 589, 596 , 603,. 610, 617, 624 , 631, 638 , 645,. 652, 659 666, 673, 680, 687 , 694, 701, 708, 715 , 722, 729 -730 3959 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 177 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92421 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92422 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92423 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92424 3 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 80 53 NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 367 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 582 , 589 596, 603, 610,. 617, 624, 631, 638, 645, 652, 659, 666, 673 , 680 687, 694, 701,. 708, 715, 722, 729- 730 554, 561, 568, 575 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358, 365, 367 3960 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 178 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92425 M 100 SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS C92426 T MASS(ES) INGUINAL REGION-LEFT WEEK 92 INGUINAL REGION-RIGHT WEEK 84 105 APPEARANCE SWOLLEN PAW-HIND-RIGHT EYES RED DISCHARGE EYE-LEFT 687, 694 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C 659C, 666C, 673C, 680C, 687C, 694C 638, 645, 652, 659, 666, 673, 680, 687, 694, 701, 708 302, 393, 484, 575, 666, 309, 400, 491, 582, 673, 316, 407, 498, 589, 680, 323, 414, 505, 596, 687, 330, 421, 512, 603, 694, 337, 428, 519, 610, 701, 344, 435, 526, 617, 708, 351, 442, 533, 624, 715, 358, 449, 540, 631, 722, 365, 372, 456, 463, 547, 554, 638, 645, 729- 730 379, 470, 561, 652, 386 477 568 659 3961 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 179 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER (CONTINUED FROM PREVIOUS PAGE) C92426 T 105 SKIN & PELAGE SORE/SCAB DORSAL PAWS-HIND GROUP: F9 NORMAL NO REMARKABLE OBSERVATIONS C92427 M MASS(ES) DORSAL WEEK 83 INGUINAL REGION-RIGHT WEEK 35 VENTRAL-ABDOMINAL-LEFT WEEK 101 63 APPEARANCE THIN SKIN & PELAGE SORE/SCAB LATERAL-ABDOMINAL-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 701, 708, 715, 722, 729- 730 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561, 568, 575 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652, 659, 666 673, 680, 687, 694, 701, 708, 715, 722, 729-730 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232 575C , 582C, 589C, 596C, 603C, 610C, 617C , 624C, 631C, 638C, 645C 652C , 659C, 666C, 673C, 680C, 687C, 694C , 701C, 708C, 715C, 722C 729- 730C 239, 246, 253, 260 , 267,, 274, 281, 288, 295, 302 , 309,, 316, 323 330, 337, 344 701C, 708C, 715C, 722C, 729-730C 428, 435 428, 435 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 99, 106 3962 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 180 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92427 M 63 NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 28 C92428 M WEEK 55 62 EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT VENTRAL-ABDOMINAL-LEFT SORE/SCAB HEAD-CRANIAL VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 51 VENTRAL-CERVICAL-LEFT WEEK 38 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 19 0C, 197C, 204C, 211C, 218C, 225C, 232C, 239C, 246C, 253C, 260C 267C, 274C, 281C, 288C, 295C, 302C, 309C, 316C, 323C, 330C, 337C 344C, 351C, 358C, 365C, 372C 379C, 386C, 393C, 400C, 407C, 414C, 421C, 428C, 435C 428 428 344 428 1, 8, 15, 22, 29, 36, 43, 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 351C, 358C, 365C, 372C, 379C, 386C , 393C , 400C, 407C, 414C , 421C 428C 260C, 267C, 274C, 281C, 288C, 295C , 302C , 309C, 316C, 323C , 330C 3963 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 181 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92428 M 62 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 38 C92429 D 62 BEHAVIOR COLD TO TOUCH ENTIRE BODY EYES PALE EYES SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS C92430 T SKIN & PELAGE SORE/SCAB PAW-HIND-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 337C 393, 400 393, 400, 407 393, 400, 407, 414 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155,. 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337,. 344, 351, 358, 365, 372, 379 386, 421, 428, 431 694, 701, 708,. 715, 722, 729- 730 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155,. 162, 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246,. 253, 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337,. 344, 351, 358, 365, 372, 379 3964 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 182 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92430 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92431 T MASS(ES) VENTRAL-ABDOMINAL WEEK 102 105 SKIN & PELAGE SORE/SCAB LATERAL-CERVICAL-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 96 INGUINAL REGION-LEFT WEEK 95 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 659, 666, 673, 680, 687 708C, 715C, 722C, 729-730C 29, 36, 43, 50, 57 1, 8,, 15, 22, 64, 71 , 78 , 85, 92, 99, 106, 113, 120, 127, 134 141, 148, 155 , 162, 169, 176, 183, 190 , 197 , 204, 211, 218, 225 232, 239, 246 , 253, 260, 267, 274, 281 , 288 , 295, 302, 309, 316 323, 330, 337 , 344, 351, 358, 365, 372 , 379 , 386, 393, 400, 407 414, 421, 428 , 435, 442, 449, 456, 463 , 470 , 477, 484, 491, 498 505, 512, 519 , 526, 533, 540, 547, 554 , 561 , 568, 575, 582, 589 596, 603, 610 , 617, 624, 631, 638, 645 , 652 666, 673, 680 , 687C, 694C, 701C, 708C, 715C , 722C, 729 -730C 659C, 666C , 6 73C, 680C, 687C, 694C , 701C, 708C, 715C, 722C 729-730C 3965 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 183 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92432 M 77 APPEARANCE HUNCHED MALOCCLUSION BEHAVIOR HYPOACTIVE <UNSPECIFIED> RESPIRATION LABORED NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 3 53 NORMAL NO REMARKABLE OBSERVATIONS 519, 526, 533, 537 29, 379, 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 484 491, 498, 505, 512, 519, 526, 533, 537 537 537 1, 8 , 15, 22, 36, 43, 50 , 57, 64, 71, 78, 85 , 92 , 99, 106, 113 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 , 19 0, 197, 204 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 , 281,. 288, 295 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 , 372 449C , 456C, 463C, 470C, 477C, 484C , 491C, 498C, 505C, 512C, 519C 526C , 533C, 537C 1, 8 , 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78 , 85 , 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176 , 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267 , 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358 , 365, 367 3966 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 184 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92434 D 91 APPEARANCE THIN NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) SHOULDER-RIGHT WEEK 55 VENTRAL-ABDOMINAL-LEFT WEEK 61 VENTRAL-ABDOMINAL-RIGHT WEEK 70 WEEK 74 582, 589, 596, 603, 610, 617, 624, 631, 633 1, 8,. 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372 379C, 386C, 393C, 400C, 407C, 414C , 421C, 428C, 435C, 442C , 449C 456C, 463C, 470C, 477C, 484C, 491C , 498C, 505C, 512C, 519C , 526C 533C,. 540C, 547C, 554C, 561C, 568C , 570C, 575C, 582C, 589C , 596C 603C, 610C, 617C, 624C, 631C, 633C 421, 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498 , 505C 512 484C,. 491C, 561C, 568C, 631C, 633C 512C, 519C, 582C,. 589C, 498C, 570C, 526C, 596C, 505C, 575C, 533C, 603C, 512C, 582C, 54 0C, 610C, 519C , 526C, 589C , 596C, 547C , 554C, 617C , 624C, 533C, 603C, 561C, 631C, 540C, 610C, 568C, 633C 547C , 554C 617C , 624C 570C , 575C 3967 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 185 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92435 1 C92436 M 4 NORMAL NO REMARKABLE OBSERVATIONS 96 APPEARANCE MALOCCLUSION THIN NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 74 VENTRAL-ABDOMINAL-LEFT WEEK 66 1, 8, 15, 22-23 225, 316, 407, 498, 589, 638, 232, 323, 414, 505, 596, 645, 239, 330, 421, 512, 603, 652, 246, 337, 428, 519, 610, 659, 253, 344, 435, 526, 617, 666 260, 351, 442, 533, 624, 267, 358, 449, 540, 631, 274, 365, 456, 547, 638, 281, 372, 463, 554, 645, 288, 379, 470, 561, 652, 295, 386, 477, 568, 659, 302, 393, 484, 575, 666 309 400 491 582 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C 666C 456C, 463C, 470C, 477C, 484C, 491C, 498C, 505C, 512C, 519C, 526C 533C, 54 0C, 54 7C, 554C, 561C, 568C, 575C, 582C, 589C, 596C, 603C 610C, 617C, 624C, 631C, 638C, 645C, 652C, 659C, 666C 3968 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 186 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92437 M 50 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL SKIN & PELAGE RED HAIR COAT DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 34 344, 348 344, 348 344, 348 344, 348 190, 197, 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274 281, 288, 295, 302, 309, 316, 323, 330, 337 1, 8,, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78,, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183 232C, 239C , 246C, 253C, 260C, 267C , 274C, 281C, 288C, 295C, 302C 309C, 316C , 323C, 330C, 337C, 344C , 348C 3969 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 187 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92438 3 53 NORMAL NO REMARKABLE OBSERVATIONS C92439 T MASS(ES) VENTRAL-ABDOMINAL WEEK 44 105 NORMAL NO REMARKABLE OBSERVATIONS C92440 D 80 APPEARANCE LIMITED USE LIMB-HIND-LEFT SWOLLEN PAW-HIND-LEFT EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295 302C,, 309C , 316C, 323C, 330C, 337C , 344C , 351C, 358C, 365C , 367C 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582, 589 , 596, 603, 610, 617, 624, 631 , 638, 645 , 652 659, 666, 673, 680 , 687, 694, 701, 708, 715, 722 , 729- 730 547, 554, 559- 560 559 559 428, 435, 442, 449, 456, 463, 470, 477, 484, 491, 498, 505, 512 3970 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 188 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92440 D 80 SKIN & PELAGE SORE/SCAB HEAD-MAXILLARY-RIGHT NORMAL NO REMARKABLE OBSERVATIONS C92441 3 MASS(ES) AXILLARY REGION-RIGHT WEEK 42 HEAD-MAXILLARY-RIGHT WEEK 66 VENTRAL-ABDOMINAL-RIGHT WEEK 45 53 SKIN & PELAGE SORE/SCAB DORSAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 39 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 519, 526, 533,. 540 , 547, 554, 559- 560 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183 , 190, 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274 , 281 288, 295, 302, 309 , 316, 323, 330, 337, 344, 351, 358 , 365, 372 379, 386, 393, 400 , 407, 414, 421, 428, 435, 442, 449 456, 463, 470, 477 , 484, 491, 498, 505C, 512C , 519C, 526C, 533C 54 0C, 54 7C, 554C, 559-560C 309, 316, 323, 330, 337, 344 316, 323, 330, 337 , 344, 351, 358, 365 , 367 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260 267, 274, 281, 288 , 295, 302, 309, 316 , 323 3971 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 189 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92441 3 53 MASS(ES) INGUINAL REGION-LEFT WEEK 48 C92442 M 68 BEHAVIOR HYPOACTIVE <UNSPECIFIED> MILD DISCHARGE NASAL RED IN COLOR EXCRETION NO FECES EYES RED DISCHARGE EYES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 330C, 337C, 344C, 351C, 358C, 365C, 367C 476 472, 476 472, 476 470, 472 476 476 470, 472 476 470, 472 476 1, 8, 15 113, 120 204, 211 295, 302 386, 393 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 3972 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 190 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92443 T 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C92444 T MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 40 105 SKIN & PELAGE SORE/SCAB PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-LEFT WEEK 80 582, 589, 596, 603 1, 8, 15 113, 120 204, 211 372, 379 463, 470 554, 561 673, 680 22, 29, 36, 43,r 50, 57, 64, 71, 78 , 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 351, 358 , 365 386, 393 , 400, 407, 414, 421 , 428, 435, 442, 449 , 456 477, 484 , 491, 498, 505, 512 , 519, 526, 533, 540 , 547 568, 575 , 610, 617, 624, 631 , 638, 645, 652, 659 , 666 687,. 694 , 701, 708, 715, 722 , 729- 730 274, 281 288, 295, 302, 309, 316, 323, 330, 337, 344 708, 715 722, 729-730 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498 , 505, 512, 519, 526 , 533, 540, 547 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638 645, 652 659, 666, 673, 680, 687, 694, 701, 708C, 715C, 722C 3973 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 191 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92444 T 105 MASS(ES) INGUINAL REGION-LEFT WEEK 80 INGUINAL REGION-RIGHT WEEK 92 VENTRAL-ABDOMINAL-RIGHT WEEK 101 C92445 M 96 APPEARANCE OBESE BEHAVIOR HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYE-LEFT SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 729- 730C 638C , 645C , 652C, 659C, 666C , 673C, 680C , 687C, 694C, 701C, 708C 715C , 722C , 729-730C 701C , 708C , 715C, 722C, 729- 730C 428, 435, 442, 449, 456, 463 , 470, 477, 484, 491, 498, 505, 512 519, 526, 533, 540, 547, 554 , 561, 568, 575, 582, 589, 596, 603 610, 617, 624, 631, 638, 645 , 652, 659, 663-666 663, 666 663-666 663 663-666 3974 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 192 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92445 M 96 NORMAL NO REMARKABLE OBSERVATIONS C92446 M 83 APPEARANCE HEAD TILT RIGHT THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYES DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421 575, 579 579 579 568, 575, 579 554, 559, 561, 568, 575,579 559, 561 568, 575, 579 554, 559, 561, 568, 575, 579 3975 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 193 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92446 M 83 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA VENTRAL-ABDOMINAL RED HAIR COAT HEAD-CRANIAL YELLOW HAIR COAT NORMAL NO REMARKABLE OBSERVATIONS M 92 APPEARANCE THIN BEHAVIOR ATAXIC <UNSPECIFIED> COLD TO TOUCH ENTIRE BODY EXCRETION FEW FECES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 579 561, 568, 575, 579 559 559 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540, 547 582, 589, 596, 603 , 610, 617, 624, 631 , 638 638 638 638 1, 8,, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 3976 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 194 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92447 M 92 NORMAL NO REMARKABLE OBSERVATIONS C92448 M 70 APPEARANCE HEAD TILT RIGHT MALOCCLUSION NORMAL NO REMARKABLE OBSERVATIONS C92449 3 53 EYES RED DISCHARGE EYE-LEFT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302 386, 393 477, 484 568, 575 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 477, 484 323, 484 1, 8, 15 113, 120 204, 211 295, 302 393, 400 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309, 316 , 330, 337, 344, 351 , 358, 365, 372, 379 , 386 407, 414 , 421, 428, 435, 442 , 449, 456, 463, 470 197, 204 302, 309, 316, 323, 330, 337 , 344 1, 8, 15 113, 120 218, 225 358, 365 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183,. 190, 211 232, 239, 246, 253, 260, 267 , 274, 281, 288, 295, 351 367 3977 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 195 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92450 M 89 APPEARANCE HEAD TILT LEFT THIN BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES SKIN & PELAGE SORE/SCAB VENTRAL-CERVICAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) INGUINAL REGION-RIGHT WEEK 40 VENTRAL-ABDOMINAL WEEK 75 VENTRAL-ABDOMINAL-LEFT WEEK 50 VENTRAL-CERVICAL WEEK 75 610, 617, 619 610, 617, 619 619 594, 596, 603, 610, 617, 619 547, 554, 561, 568 , 575, 582, 589, 594 , 596, 603, 610, 617, 619 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 414, 421, 428 435, 442, 449, 456 , 463, 470, 477, 484 , 491, 498, 505, 512 274, 281, 288, 295 , 302, 309, 316, 323 , 330, 337 519, 526, 533,. 540 , 547, 554C, 561C, 568C, 575C, 582C, 589C 594C, 596C , 603C, 610C, 617C, 619C 344, 351, 358, 365 , 372, 379, 386, 393 , 400, 407 519, 526, 533,. 540 , 547, 554, 561, 568 , 575 3978 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 196 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92450 M 89 MASS(ES) VENTRAL-CERVICAL WEEK 77 WEEK 84 T APPEARANCE SWOLLEN PAW-HIND-RIGHT PAWS-HIND NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 48 INGUINAL REGION-RIGHT WEEK 42 T SKIN & PELAGE SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 533, 540, 547, 554 , 561, 568, 575 582C, 589C , 594C, 596C, 603C, 610C , 617C , 619C 638, 645, 652, 659 666, 673, 680, 687 , 694, 701, 708, 715, 722, 729- 730 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 407 414, 421, 428, 435 , 442, 449, 456, 463, 470, 477, 484, 491 , 498 505, 512, 519, 526 , 533, 540, 547, 554, 561, 568, 575, 582 , 589 596, 603, 610, 617 , 624, 631 330, 337, 344, 351 , 358, 365, 372, 379, 386, 393, 400 288, 295, 302, 309 , 316, 323, 330, 337 659, 666, 673, 680 , 687, 694, 701, 708, 715, 722, 729- 730 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253, 260, 267, 274, 281 , 288 3979 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 197 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92452 T 105 NORMAL NO REMARKABLE OBSERVATIONS C92453 M 100 EXCRETION FEW FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL WEEK 61 VENTRAL-CERVICAL WEEK 80 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435, 442, 449, 456, 463, 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 568, 575, 582, 589, 596, 603, 610, 617, 624, 631, 638, 645, 652 649, 652, 659, 666 649, 652, 659, 666, 673, 680, 687, 694 649, 652, 659, 666, 673, 680, 687, 694 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407 , 414, 456, 463, 470 , 477, 484, 491, 498 , 505 512, 519, 526, 533 , 540, 547 421, 428, 435, 442, 449 554, 561, 568, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C 631C, 638C , 645C, 649C, 652C, 65 9C, 666C, 673C, 680C, 68 7C, 694C 3980 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 198 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92454 M 81 BEHAVIOR ATAXIC <UNSPECIFIED> EXCRETION FEW FECES EYES SQUINTED PERI-ORBITAL SKIN & PELAGE SORE/SCAB VENTRAL-ABDOMINAL NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 VENTRAL-CERVICAL WEEK 54 566 561, 566 566 484, 491, 498, 505, 512, 519, 526, 533, 540, 547, 554, 561 , 566 1, 8, 15, 22, 29, 36, 43 , 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127, 134, 141, 148, 155, 162, 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281 , 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365 421C, 428C, 435C, 442C, 44 9C, 456C , 463C, 470C, 477C, 484C , 491C 498C, 505C, 512C, 519C, 526C, 533C , 540C, 547C, 554C, 561C , 566C 372C, 379C, 386C, 393C, 400C, 407C , 414C, 421C, 428C, 435C , 442C 449C, 456C, 463C, 470C, 477C, 484C , 491C, 498C, 505C, 512C , 519C 526C, 533C, 540C, 547C, 554C, 561C , 566C 3981 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 199 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92455 M 103 APPEARANCE HEAD TILT LEFT BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NO FECES SKIN & PELAGE RED HAIR COAT HEAD-ENTIRE SHOULDER-LEFT NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 101 718 718 708, 715 718 706 701, 706 708, 715, 718 706, 708 715, 718 1, 8, 15 113, 120 204, 211 295, 302 386, 393 477, 484 568, 575 659, 666 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 673, 680, 687, 694 701C, 70 6C, 708C, 715C, 718C 3982 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 200 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92456 T 105 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS C92457 M MASS(ES) AXILLARY REGION-RIGHT WEEK 88 85 APPEARANCE HEAD TILT RIGHT THIN EXCRETION FEW FECES SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA RED HAIR COAT HEAD-ENTIRE YELLOW HAIR COAT 722 1, 8,, 15, 22, 29, 36, 43 , 50, 57, 64, 71 , 78, 85 , 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162, 169, 176 , 183, 190 , 197 204, 211, 218,. 225, 232, 239, 246, 253, 260, 267 , 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344, 351, 358 , 365, 372 , 379 386, 393, 400,. 407, 414, 421, 428, 435, 442, 449 , 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526, 533, 540 , 547, 554 , 561 568, 575, 582,. 589, 596, 603 610C, 617C , 624C, 631C, 638C, 645C , 652C , 659C, 666C, 673C , 680C 687C, 694C , 701C, 708C, 715C, 722C , 729- 730C 582, 589 582, 589 561, 568 582, 589 568 568, 575 3983 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 201 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92457 M 85 NORMAL NO REMARKABLE OBSERVATIONS C92458 M MASS(ES) AXILLARY REGION-LEFT WEEK 84 INGUINAL REGION-LEFT WEEK 80 68 APPEARANCE HUNCHED THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> RECUMBENT LATERAL EXCRETION FEW FECES NO FECES EYES RED DISCHARGE EYE-LEFT EYES DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85 113, 120, 127, 134 , 141, 148, 155, 162 , 169, 176 204, 211, 218, 225 , 232, 239, 246, 253 , 260, 267 295, 302, 309, 316 , 323, 330, 337, 344 , 351, 358 386, 393, 400, 407 , 414, 421, 428, 435 , 442, 449 477, 484, 491, 498 , 505, 512, 519, 526 , 533, 540 92, 99, 106 183, 190, 197 274, 281, 288 365, 372, 379 456, 463, 470 547 582, 589 554, 561, 568, 575 465, 470 465, 470 470 470 443, 449, 456, 463, 465, 470 449, 465 428, 435, 439, 442-443, 449, 456, 463, 465 465, 470 3984 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 202 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92458 M 68 SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-LEFT WEEK 51 M 100 EXCRETION NONFORMED FECES NORMAL NO REMARKABLE OBSERVATIONS MASS(ES) AXILLARY REGION-RIGHT WEEK 70 INGUINAL REGION-RIGHT WEEK 92 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 443, 449, 456,. 463, 465, 470 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 407, 414, 421 351, 358, 365,. 372, 379, 386, 393, 400 582, 589, 596, 603 1, 8,r 15, 22, 29, 36, 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190, 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281, 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372, 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463, 470 477 484C, 491C, 498C, 505C, 512C, 519C, 526C, 533C, 54 0C, 54 7C, 554C 561C, 568C, 575C, 582C, 589C, 596C, 603C, 610C, 617C, 624C, 631C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 638C, 645C, 652C, 659C, 666C, 673C, 680C, 687C, 694C 3985 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 203 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92460 M 97 APPEARANCE HUNCHED THIN BEHAVIOR ATAXIC <UNSPECIFIED> HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES NONFORMED FECES EYES RED DISCHARGE EYES SQUINTED PERI-ORBITAL-LEFT SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SORE/SCAB PAW-HIND-RIGHT NORMAL NO REMARKABLE OBSERVATIONS 679 666, 673, 679 679 666, 673, 679 679 575 666, 673, 679 638, 645, 652, 659, 666, 673, 679 505, 512 638, 645, 652, 659, 666, 673, 679 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421 3986 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 204 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92460 M 97 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 C92461 M VENTRAL-ABDOMINAL-RIGHT WEEK 64 61 BEHAVIOR HYPOACTIVE <UNSPECIFIED> SENSITIVE TO TOUCH DISCHARGE NASAL RED IN COLOR EXCRETION FEW FECES EYES RED DISCHARGE EYES SKIN & PELAGE PALE ENTIRE BODY NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 42 8C, 435C, 442C, 44 9C, 456C, 463C, 470C, 477C, 484C, 491C, 498C 505C, 512C, 519C, 52 6C, 53 3C, 54 0C, 54 7C, 554C, 561C, 56 8C, 575C 582C, 58 9C, 596C, 603C, 610C, 617C, 624C, 631C, 638C, 64 5C, 652C 659C, 666C, 673C, 679C 442C, 449C 425 424-425 421, 424-425 421, 424- 425 424-425 424-425 1, 8, 15, 22, 29, 36, 43,, 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,, 134, 141, 148, 155,. 162, 169, 176, 183,. 190, 197 3987 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 205 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 (CONTINUED FROM PREVIOUS PAGE) C92461 M 61 NORMAL NO REMARKABLE OBSERVATIONS C92462 M 99 APPEARANCE THIN BEHAVIOR HYPOACTIVE <UNSPECIFIED> EXCRETION FEW FECES EYES RED DISCHARGE EYES NORMAL NO REMARKABLE OBSERVATIONS DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS 204, 211, 218, 225, 232, 239, 246, 253, 260, 267, 274, 281, 288 295, 302, 309, 316, 323, 330, 337, 344, 351, 358, 365, 372, 379 386, 393, 400, 407, 414 673, 680, 687 687 687 687 1, 8,r 15, 22, 29, 36 , 43,r 50, 57, 64, 71, 78, 85, 92, 99, 106 113, 120, 127,. 134, 141, 148, 155, 162 , 169, 176, 183, 190 , 197 204, 211, 218, 225, 232, 239, 246, 253 , 260, 267, 274, 281 , 288 295, 302, 309,. 316, 323, 330, 337, 344 , 351, 358, 365, 372 , 379 386, 393, 400, 407, 414, 421, 428, 435 , 442, 449, 456, 463 , 470 477, 484, 491, 498, 505, 512, 519, 526 , 533, 540, 547, 554 , 561 568, 575, 582, 589, 596, 603, 610, 617 , 624, 631, 638, 645 , 652 659, 666 3988 Covance 6329-212 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 206 CATEGORY ANIMAL DEATH WK OF KEYWORD NUMBER CODE DEATH QUALIFIER GROUP: F9 DOSE: 1 PPM DAYS 1-730 - OBSERVED DURING THE DAYS LISTED BELOW; 'C' - COMMENTS LISTED AT END OF OBSERVATIONS C92463 1 C92464 2 C92465 2 C92466 2 C92467 1 C92468 2 C92469 2 C92470 1 4 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 14 NORMAL NO REMARKABLE OBSERVATIONS 4 NORMAL NO REMARKABLE OBSERVATIONS 1, 8, 15, 22 -23 1, 8, 15, 22 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 1, 8, 15, 22 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 1, 8, 15, 22 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 1, 8, 15, 22 -23 1, 8, 15, 22 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 1, 8, 15, 22 , 29, 36, 43, 50, 57, 64, 71, 78, 85, 92-93 1, 8, 15, 22 -23 3989 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 35 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 36 MASS(ES) 37 VENTRAL-ABDOMINAL WEEK 35 38 MASS(ES) 41 VENTRAL-ABDOMINAL WEEK 35 42 MASS(ES) 44 VENTRAL-ABDOMINAL WEEK 35 45 MASS(ES) VENTRAL-ABDOMINAL WEEK 35 46 MASS(ES) 49 VENTRAL-ABDOMINAL WEEK 35 50 MASS(ES) 51 VENTRAL-ABDOMINAL WEEK 35 76 MASS(ES) 78 VENTRAL-ABDOMINAL-LEFT WEEK 76 10 X 10MM 20 X 10MM 25 X 25MM 40 X 40MM 45 X 40MM 15 X 15MM 10 X 10MM 35 X 20MM 3990 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 76 80 MASS(ES) 81 VENTRAL-ABDOMINAL-LEFT WEEK 76 73 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 70 74 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 70 75 MASS(ES) 78 VENTRAL-CERVICAL-LEFT WEEK 70 79 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 70 80 MASS(ES) 81 VENTRAL-CERVICAL-LEFT WEEK 70 80 MASS(ES) 87 AXILLARY REGION-LEFT WEEK 79 40 X 30MM 50 X 45MM 10 X 10MM 15 X 15MM 20 X 15MM 35 X 35MM 25 X 25MM 10 X 10MM 3991 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 84 MASS(ES) 87 INGUINAL REGION-LEFT WEEK 84 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 74 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 75 MASS(ES) 79 AXILLARY REGION-RIGHT WEEK 73 80 MASS(ES) 83 AXILLARY REGION-RIGHT WEEK 73 84 MASS(ES) 87 AXILLARY REGION-RIGHT WEEK 73 88 MASS(ES) 96 AXILLARY REGION-RIGHT WEEK 73 97 MASS(ES) 99 AXILLARY REGION-RIGHT WEEK 73 20 X 20MM 10 X 10MM 15 X 15MM 20 X 25MM 30 X 35MM 35 X 40MM 65 X 45MM 65 X 55MM 3992 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 100 MASS(ES) 102 AXILLARY REGION-RIGHT WEEK 73 103 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 104 MASS(ES) 104 AXILLARY REGION-RIGHT WEEK 73 104 MASS(ES) AXILLARY REGION-RIGHT WEEK 73 92 MASS(ES) 98 VENTRAL-ABDOMINAL-RIGHT WEEK 92 99 MASS(ES) 102 VENTRAL-ABDOMINAL-RIGHT WEEK 92 103 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 92 104 MASS(ES) 104 VENTRAL-ABDOMINAL-RIGHT WEEK 92 70 X 70MM 85 X 85MM 100 X 85MM 100 X 85MM 30 X 40MM 10 X 10MM 30 X 20MM 35 X 20MM 3993 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92408 F9 722 104 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 92 F9 519 75 TO 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 554 80 TO 568 82 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 575 83 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 582 84 TO 603 87 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 610 88 TO 631 91 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 638 92 TO 673 97 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 679 97 TO 691 99 MASS(ES) AXILLARY REGION-RIGHT WEEK 74 35 X 20MM 10 X 10MM 15 X 15MM 15 X 20MM 20 X 20MM 25 X 25MM 30 X 30MM 40 X 40MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 211 3994 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92409 C92410 C92411 F9 519 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 526 76 TO 554 80 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 75 F9 729 105 MASS(ES) TO 730 105 VENTRAL-ABDOMINAL-LEFT WEEK 103 610 88 MASS(ES) TO 708 102 VENTRAL-CERVICAL WEEK 88 715 103 MASS(ES) TO 722 104 VENTRAL-CERVICAL WEEK 88 729 105 MASS(ES) TO 730 105 VENTRAL-CERVICAL WEEK 88 F9 302 44 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 309 45 TO 323 47 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 20 X 35MM 30 X 35MM 10 X 10MM 20 X 25MM 30 X 30MM 40 X 35MM 30 X 10MM 10 X 10MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 212 3995 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92411 C92413 C92414 F9 330 48 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 337 49 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 44 F9 666 96 MASS(ES) TO 710 102 AXILLARY REGION-RIGHT WEEK 96 F9 260 38 MASS(ES) LIMB-FRONT-RIGHT WEEK 35 267 39 TO 281 41 MASS(ES) LIMB-FRONT-RIGHT WEEK 35 288 42 TO 295 43 MASS(ES) PAW-HIND-RIGHT WEEK 42 309 45 TO 337 49 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 344 50 TO 365 53 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 15 X 10MM 15 X 10MM 55 X 50MM 20 X 20MM 40 X 25MM 65 X 45MM 70 X 50MM 70 X 60MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 213 3996 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92414 C92416 C92419 F9 372 54 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 379 55 TO 407 59 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 414 60 TO 435 63 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 44 F9 582 84 TO 603 87 MASS(ES) INGUINAL REGION-RIGHT WEEK 84 519 75 TO 547 79 MASS(ES) TAIL-PROXIMAL WEEK 75 554 80 MASS(ES) TO 708 102 TAIL-PROXIMAL WEEK 75 715 103 MASS(ES) TO 730 105 TAIL-PROXIMAL WEEK 75 F9 701 101 MASS(ES) TO 715 103 AXILLARY REGION-RIGHT WEEK 101 90 X 70MM 95 X 80MM 100 X 80MM 20 X 20MM 10 X 10MM 10 X 15MM 20 X 15MM 20 X 20MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 214 3997 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 10 MASS(ES) 10 AXILLARY REGION-RIGHT WEEK 101 59 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 60 MASS(ES) 61 AXILLARY REGION-RIGHT WEEK 59 62 MASS(ES) 63 AXILLARY REGION-RIGHT WEEK 59 64 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 65 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 66 MASS(ES) 68 AXILLARY REGION-RIGHT WEEK 59 69 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 25 X 25MM 15 X 15MM 25 X 20MM 25 X 30MM 45 X 20MM 35 X 55MM 35 X 40MM 40 X 50MM 3998 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 70 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 71 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 72 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 73 MASS(ES) AXILLARY REGION-RIGHT WEEK 59 56 MASS(ES) 58 VENTRAL-ABDOMINAL WEEK 56 92 MASS(ES) 98 INGUINAL REGION-LEFT WEEK 92 40 X 50MM 40 X 50MM 40 X 50MM 55 X 60MM 55 X 60MM 40 X 50MM 15 X 15MM 25 X 20MM 3999 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 99 MASS(ES) 100 INGUINAL REGION-LEFT WEEK 92 84 MASS(ES) 91 INGUINAL REGION-RIGHT WEEK 84 92 MASS(ES) 96 INGUINAL REGION-RIGHT WEEK 84 97 MASS(ES) 100 INGUINAL REGION-RIGHT WEEK 84 83 MASS(ES) 105 DORSAL WEEK 83 101 MASS(ES) 102 VENTRAL-ABDOMINAL-LEFT WEEK 101 103 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 101 104 MASS(ES) 105 VENTRAL-ABDOMINAL-LEFT WEEK 101 30 X 25MM 10 X 10MM 25 X 40MM 50 X 50MM 10 X 10MM 40 X 40MM 60 X 45MM 70 X 65MM 4000 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F9 190 28 197 29 TO 204 30 211 31 TO 218 32 225 33 TO 232 34 239 35 TO 281 41 288 42 TO 309 45 316 46 TO 337 49 344 50 TO 365 53 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 20 X10MM 20 X 20MM 30 X 30MM 45 X 35MM 50 X 45MM 75 X 70MM 110 X 95MM 100 X 70MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 218 4001 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92427 C92428 F9 372 54 379 55 TO 435 63 F9 351 51 TO 365 53 372 54 379 55 TO 407 59 414 60 TO 421 61 428 62 TO 428 62 428 62 MASS(ES) AXILLARY REGION-RIGHT WEEK 28 MASS(ES) AXILLARY REGION-RIGHT WEEK 55 MASS(ES) AXILLARY REGION-LEFT WEEK 51 MASS(ES) AXILLARY REGION-LEFT WEEK 51 MASS(ES) AXILLARY REGION-LEFT WEEK 51 MASS(ES) AXILLARY REGION-LEFT WEEK 51 MASS(ES) AXILLARY REGION-LEFT WEEK 51 MASS(ES) AXILLARY REGION-LEFT WEEK 51 100 X 90MM 110 X 100MM 20 X 20MM 25 X 25MM 40 X 30MM 75 X 60MM 85 X 75MM 85 X 75MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 219 4002 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92428 C92430 C92431 F9 260 38 TO 267 39 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 38 274 40 TO 281 41 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 38 288 42 TO 337 49 MASS(ES) VENTRAL-CERVICAL-LEFT WEEK 38 F9 708 102 MASS(ES) TO 715 103 VENTRAL-ABDOMINAL WEEK 102 722 104 MASS(ES) TO 730 105 VENTRAL-ABDOMINAL WEEK 102 F9 687 99 MASS(ES) TO 694 100 AXILLARY REGION-RIGHT WEEK 96 701 101 MASS(ES) TO 722 104 AXILLARY REGION-RIGHT WEEK 96 729 105 MASS(ES) TO 730 105 AXILLARY REGION-RIGHT WEEK 96 20 X 15MM 20 X 20MM 25 X 30MM 30 X 35MM 40 X 40MM 20 X 10MM 30 X 30MM 40 X 30MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 220 4003 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92431 C92432 F9 659 95 TO 666 96 MASS(ES) INGUINAL REGION-LEFT WEEK 95 673 97 TO 687 99 MASS(ES) INGUINAL REGION-LEFT WEEK 95 694 100 MASS(ES) INGUINAL REGION-LEFT WEEK 95 701 101 MASS(ES) TO 730 105 INGUINAL REGION-LEFT WEEK 95 F9 449 65 TO 463 67 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 470 68 TO 477 69 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 484 70 TO 498 72 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 505 73 MASS(ES) VENTRAL-THORACIC-LEFT WEEK 65 20 X 20MM 35 X 35MM 40 X 40MM 50 X 50MM 10 X 10MM 20 X 20MM 30 X 30MM 40 X 40MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 221 4004 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 74 MASS(ES) 75 VENTRAL-THORACIC-LEFT WEEK 65 76 MASS(ES) 77 VENTRAL-THORACIC-LEFT WEEK 65 55 MASS(ES) 59 SHOULDER-RIGHT WEEK 55 60 MASS(ES) 64 SHOULDER-RIGHT WEEK 55 65 MASS(ES) 69 SHOULDER-RIGHT WEEK 55 70 MASS(ES) 72 SHOULDER-RIGHT WEEK 55 73 MASS(ES) 75 SHOULDER-RIGHT WEEK 55 76 MASS(ES) 79 SHOULDER-RIGHT WEEK 55 45 X 45MM 50 X 45MM 30 X 35MM 45 X 35MM 60 X 40MM 60 X 50MM 70 X 55MM 75 X 55MM 4005 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F9 554 80 TO 617 89 624 90 TO 633 91 505 73 484 70 TO 498 72 505 73 512 74 TO 540 78 547 79 TO 596 86 603 87 TO 617 89 MASS(ES) SHOULDER-RIGHT WEEK 55 MASS(ES) SHOULDER-RIGHT WEEK 55 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 61 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 75 X 60MM 80 X 65MM 10 X 10MM 10 X 10MM 20 X 20MM 25 X 25MM POSTERIOR 30 X 30MM 35 X 35MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 223 4006 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT F9 624 90 TO 633 91 512 74 TO 519 75 526 76 TO 540 78 547 79 554 80 TO 575 83 582 84 TO 596 86 603 87 TO 617 89 624 90 TO 633 91 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 70 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 74 40 X 50MM ANTERIOR 15 X 15 ANTERIOR 20 X 20MM 40 X 30MM 50 X 40MM 60 X 40MM 70 X 50MM 100 X 75MM POSTERIOR Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 224 4007 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 74 MASS(ES) AXILLARY REGION-LEFT WEEK 74 75 MASS(ES) 79 AXILLARY REGION-LEFT WEEK 74 80 MASS(ES) 82 AXILLARY REGION-LEFT WEEK 74 83 MASS(ES) AXILLARY REGION-LEFT WEEK 74 84 MASS(ES) 96 AXILLARY REGION-LEFT WEEK 74 66 MASS(ES) 68 VENTRAL-ABDOMINAL-LEFT WEEK 66 69 MASS(ES) 73 VENTRAL-ABDOMINAL-LEFT WEEK 66 74 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 10 X 10MM 20 X 20MM 35 X 35MM 35 X 40MM 40 X 50MM 15 X 10MM 20 X 20MM 25 X 30MM 4008 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 75 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 76 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 77 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 78 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 79 MASS(ES) VENTRAL-ABDOMINAL-LEFT WEEK 66 80 MASS(ES) 91 VENTRAL-ABDOMINAL-LEFT WEEK 66 92 MASS(ES) 93 VENTRAL-ABDOMINAL-LEFT WEEK 66 94 MASS(ES) 96 VENTRAL-ABDOMINAL-LEFT WEEK 66 30 X 30MM 35 X 35MM 35 X 35MM 35 X 35MM 35 X 35MM 45 X 45MM 50 X 50MM 50 X 60MM 4009 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 34 MASS(ES) 37 VENTRAL-ABDOMINAL-LEFT WEEK 34 38 MASS(ES) 41 VENTRAL-ABDOMINAL-LEFT WEEK 34 42 MASS(ES) 45 VENTRAL-ABDOMINAL-LEFT WEEK 34 46 MASS(ES) 49 VENTRAL-ABDOMINAL-LEFT WEEK 34 50 MASS(ES) 50 VENTRAL-ABDOMINAL-LEFT WEEK 34 44 MASS(ES) 53 VENTRAL-ABDOMINAL WEEK 44 73 MASS(ES) 80 HEAD-MAXILLARY-RIGHT WEEK 66 48 MASS(ES) 53 INGUINAL REGION-LEFT WEEK 48 20 X 10MM 30 X 15MM 35 X 20MM 45 X 30MM 30 X 20MM 10 X 10MM 10 X 10MM 20 X 15MM 4010 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 102 MASS(ES) INGUINAL REGION-LEFT WEEK 80 103 MASS(ES) 105 INGUINAL REGION-LEFT WEEK 80 92 MASS(ES) 96 INGUINAL REGION-RIGHT WEEK 92 97 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 98 MASS(ES) 99 INGUINAL REGION-RIGHT WEEK 92 100 MASS(ES) 105 INGUINAL REGION-RIGHT WEEK 92 101 MASS(ES) 104 VENTRAL-ABDOMINAL-RIGHT WEEK 101 105 MASS(ES) 105 VENTRAL-ABDOMINAL-RIGHT WEEK 101 10 X 10MM 20 X 20MM 15 X 15MM 20 X 30MM 30 X 30MM 35 X 35MM 40 X 40MM 55 X 50MM 4011 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE- TEXT C92450 C92453 C92454 F9 554 80 TO 561 81 MASS(ES) VENTRAL-ABDOMINAL WEEK 75 568 82 TO 619 89 MASS(ES) VENTRAL-ABDOMINAL WEEK 75 582 84 TO 619 89 MASS(ES) VENTRAL-CERVICAL WEEK 84 F9 575 83 TO 631 91 MASS(ES) VENTRAL-CERVICAL WEEK 80 638 92 TO 680 98 MASS(ES) VENTRAL-CERVICAL WEEK 80 687 99 MASS(ES) TO 694 100 VENTRAL-CERVICAL WEEK 80 F9 421 61 TO 442 64 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 449 65 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 61 10 X 10MM 20 X 20MM 30 X 30MM 10 X 10MM 20 X 30MM 30 X 30MM 20 X 15MM 40 X 25MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 229 4012 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT 66 MASS(ES) 67 VENTRAL-ABDOMINAL-RIGHT WEEK 61 68 MASS(ES) 72 VENTRAL-ABDOMINAL-RIGHT WEEK 61 73 MASS(ES) 75 VENTRAL-ABDOMINAL-RIGHT WEEK 61 76 MASS(ES) 81 VENTRAL-ABDOMINAL-RIGHT WEEK 61 54 MASS(ES) VENTRAL-CERVICAL WEEK 54 55 MASS(ES) 59 VENTRAL-CERVICAL WEEK 54 60 MASS(ES) 64 VENTRAL-CERVICAL WEEK 54 65 MASS(ES) 69 VENTRAL-CERVICAL WEEK 54 15 X 15MM 20 X 20MM 30 X 25MM 25 X 25MM 10 X 10MM 25 X 25MM 30 X 30MM 40 X 25MM 4013 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1 N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS CATEGORY KEYWORD WK QUALIFIER FREE-TEXT COMMENT 70 MASS(ES) 75 VENTRAL-CERVICAL WEEK 54 76 MASS(ES) 81 VENTRAL-CERVICAL WEEK 54 101 MASS(ES) 103 AXILLARY REGION-LEFT WEEK 101 88 MASS(ES) 91 AXILLARY REGION-RIGHT WEEK 88 92 MASS(ES) 99 AXILLARY REGION-RIGHT WEEK 88 100 MASS(ES) 102 AXILLARY REGION-RIGHT WEEK 88 103 MASS(ES) 105 AXILLARY REGION-RIGHT WEEK 88 70 MASS(ES) 72 AXILLARY REGION-RIGHT WEEK 70 40 X 35MM 40 X 40MM 45 X 40MM 10 X 10MM 25 X 25MM 45 X 30MM 45 X 40MM 10 X 10MM 4014 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92459 C92460 F9 505 73 TO 519 75 MASS(ES) AXILLARY REGION-RIGHT WEEK 70 526 76 TO 547 79 MASS(ES) AXILLARY REGION-RIGHT WEEK 70 554 80 MASS(ES) TO 694 100 AXILLARY REGION-RIGHT WEEK 70 638 92 TO 673 97 MASS(ES) INGUINAL REGION-RIGHT WEEK 92 680 98 MASS(ES) TO 694 100 INGUINAL REGION-RIGHT WEEK 92 F9 428 62 TO 449 65 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 456 66 TO 498 72 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 505 73 TO 519 75 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 20 X 20MM 25 X 25MM 35 X 35MM 20 X 30MM 30 X 30MM 30 X 30MM 30 X 40MM 30 X 30MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 232 4015 Appendix 2 Individual Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS STUDY ANIMAL GROUP DAY WK CATEGORY KEYWORD QUALIFIER FREE-TEXT COMMENT C92460 F9 526 76 TO 547 79 554 80 TO 582 84 589 85 TO 679 97 442 64 TO 449 65 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 MASS(ES) VENTRAL-ABDOMINAL WEEK 62 MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 64 35 X 30MM 40 X 30MM 40 X 35MM 10 X 10MM Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 233 4016 APPENDIX 3 In d iv id u a l B o d y W e ig h t D a ta (g) Covance 6329-212 Covance 6329-228 3M T-6316.1 4017 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 8 - 0 PPM C92191 205 255 297 332 355 370 383 398 C92192 194 246 302 349 C92193 204 261 307 352 381 411 431 450 C92194 192 252 301 345 376 400 424 435 C92195 181 228 267 305 330 358 380 399 C92196 199 258 300 338 C92197 234 285 332 373 402 433 451 467 C92198 201 251 298 338 366 396 415 420 C92199 163 205 244 283 311 339 361 377 C92200 166 221 275 324 350 377 396 407 C92201 218 269 310 346 375 405 427 442 C92202 184 241 286 322 347 373 393 413 C92203 215 275 327 377 415 450 472 488 C92204 208 252 288 319 349 367 384 398 C92205 215 279 349 405 445 485 523 545 C92206 187 241 294 332 357 392 416 431 C92207 217 273 318 353 C92208 222 273 327 363 391 425 451 462 C92209 172 218 260 297 330 361 385 398 C92210 209 278 330 382 409 445 472 486 C92211 174 233 276 319 353 378 403 418 C92212 204 246 280 309 328 343 356 367 C92213 204 253 304 349 381 409 423 444 C92214 209 268 322 372 411 442 471 491 C92215 214 263 306 345 381 405 436 464 C92216 193 248 290 339 C92217 200 256 310 355 385 415 436 455 C92218 182 236 288 333 361 386 401 412 C92219 226 289 354 397 437 476 505 519 C92220 215 276 326 363 395 419 443 458 C92221 170 220 268 305 329 348 361 371 Covance 6329-228 3M T-6316.1 PAGE: 1 WEEK WEEK 910 414 423 465 475 454 468 413 430 484 504 416 440 392 406 424 449 461 472 422 442 503 524 412 426 572 595 458 483 477 490 420 370 505 501 431 443 371 383 454 469 517 539 480 504 472 487 426 447 544 568 477 498 380 388 4018 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 8 - 0 PPM C92222 206 265 304 347 380 412 429 455 C92223 194 246 291 331 358 384 405 418 C92224 220 279 327 376 409 437 462 482 C92225 180 230 283 329 357 384 411 431 C92226 209 259 296 337 370 395 417 438 C92227 188 252 300 346 387 417 444 468 C92228 185 236 282 321 344 372 394 409 C92229 198 255 314 366 405 435 461 487 C92230 176 235 293 347 375 409 445 466 C92231 204 260 316 363 395 426 458 464 C92232 234 302 355 396 440 472 501 520 C92233 180 233 280 319 353 380 409 422 C92234 219 276 333 376 410 434 456 479 C92235 203 249 279 305 321 345 364 376 C92236 199 246 287 320 345 366 384 394 C92237 225 293 354 399 437 471 495 517 C92238 236 289 334 376 412 437 455 477 C92239 221 276 324 360 386 414 446 470 C92240 211 269 324 364 395 415 445 466 C92241 185 240 283 318 344 368 389 402 C92242 199 262 319 366 401 438 460 482 C92243 220 281 340 387 422 459 488 506 C92244 204 260 317 361 399 432 463 484 C92245 226 290 344 388 421 447 469 493 C92246 182 234 287 327 341 357 379 395 C92247 235 298 355 402 431 464 496 515 C92248 227 285 335 382 417 449 479 506 C92249 213 272 324 357 374 398 418 434 C92250 200 261 312 354 388 421 447 470 C92251 212 271 328 364 394 424 447 459 C92252 175 232 283 315 339 362 394 411 Covance 6329-228 3M T-6316.1 PAGE: 2 WEEK WEEK 910 468 477 437 453 501 515 450 472 459 468 481 495 423 444 503 527 482 470 478 493 542 566 435 453 501 524 394 415 411 423 533 553 489 507 485 510 491 509 416 431 500 520 525 547 511 538 519 541 414 427 538 562 522 545 457 473 488 503 480 492 428 438 4019 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 8 - 0 PPM C92253 225 285 335 374 402 426 456 483 C92254 231 288 338 386 422 456 477 498 C92255 174 235 288 327 345 360 372 378 C92256 213 281 333 387 420 451 475 494 C92257 227 289 341 388 418 448 478 494 C92258 195 256 309 356 392 430 456 479 C92259 228 290 335 379 C92260 215 273 322 368 391 412 438 452 GROUP: MALE 9 - 1 PPM C92261 185 237 282 323 351 376 396 408 C92262 213 272 331 382 419 453 479 492 C92263 224 287 332 376 405 436 470 493 C92264 199 255 307 353 C92265 236 301 353 399 430 465 479 468 C92266 176 224 271 315 345 382 404 412 C92267 223 276 322 359 388 408 425 436 C92268 228 293 354 401 449 490 517 552 C92269 193 254 318 368 409 439 471 491 C92270 211 267 292 304 319 338 360 378 C92271 236 296 353 402 443 477 511 531 C92272 181 235 278 310 340 367 385 400 C92273 191 258 312 365 403 438 468 489 C92274 184 239 291 334 364 396 423 439 C92275 187 238 280 318 342 366 382 397 C92276 178 229 275 320 355 391 419 432 C92277 216 274 331 376 418 453 486 506 C92278 215 277 326 358 380 398 421 439 C92279 205 256 297 340 371 403 428 446 C92280 199 256 307 344 Covance 6329-228 3M T-6316.1 PAGE: 3 WEEK WEEK 910 506 522 518 540 395 409 522 537 515 525 497 507 470 487 424 436 515 527 513 530 490 516 434 454 445 463 577 604 510 527 394 412 552 573 416 432 510 513 453 468 410 425 453 466 528 553 456 478 462 482 4020 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 9 - 1 PPM C92281 209 256 300 341 380 402 424 442 C92282 190 247 300 341 376 411 438 453 C92283 200 250 293 330 360 391 419 436 C92284 216 277 319 352 C92285 208 280 341 389 433 465 500 524 C92286 219 272 317 351 389 424 443 468 C92287 198 262 314 359 395 429 456 472 C92288 221 282 332 374 412 439 466 486 C92289 195 256 307 351 384 417 446 459 C92290 207 259 304 342 370 393 407 430 C92291 197 257 304 337 365 388 406 414 C92292 183 226 263 290 317 346 370 383 C92293 206 262 303 346 377 410 431 447 C92294 214 272 315 346 375 403 424 438 C92295 188 251 308 362 402 436 467 482 C92296 180 234 275 314 342 371 398 418 C92297 171 221 267 301 332 357 372 382 C92298 222 285 343 392 439 479 513 529 C92299 180 232 269 309 338 365 383 398 C92300 214 267 319 353 C92301 218 285 331 370 393 432 460 468 C92302 220 287 352 411 456 501 538 564 C92303 196 253 301 348 380 416 441 455 C92304 215 283 338 376 407 438 465 480 C92305 225 289 343 378 409 432 461 483 C92306 213 275 325 363 392 421 436 451 C92307 165 222 268 310 344 380 405 426 C92308 169 226 274 308 333 354 375 385 C92309 213 271 315 357 373 390 413 423 C92310 212 275 336 384 425 459 496 505 C92311 213 273 323 360 384 425 448 465 Covance 6329-228 3M T-6316.1 PAGE: 4 WEEK WEEK 910 460 483 470 482 447 465 545 570 484 502 489 499 503 526 480 500 444 467 427 440 397 409 469 485 464 489 507 524 437 450 397 404 552 568 407 426 493 507 587 612 471 489 502 521 510 512 465 480 446 470 406 420 445 458 531 551 483 500 4021 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 9 - 1 PPM C92312 213 262 293 331 346 368 380 393 C92313 225 281 325 357 378 402 436 448 C92314 240 291 339 378 396 424 440 454 C92315 205 269 328 365 399 425 450 465 C92316 190 243 290 327 361 385 406 420 C92317 226 282 334 364 409 442 471 484 C92318 167 227 275 324 362 398 425 437 C92319 202 267 317 364 390 419 436 450 C92320 189 248 298 340 373 406 437 458 C92321 172 227 270 306 337 366 382 393 C92322 170 227 267 303 334 362 384 400 C92323 199 266 318 369 416 446 480 495 C92324 220 275 321 360 389 422 439 455 C92325 228 291 344 392 437 469 504 532 C92326 170 226 286 327 366 401 425 437 C92327 210 272 322 362 394 417 440 457 C92328 194 255 309 346 384 415 439 463 C92329 167 218 271 314 C92330 229 288 339 381 424 450 474 500 GROUP: FEMALE 8 - 0 PPM C92331 144 175 200 217 236 254 251 267 C92332 146 179 194 209 C92333 142 162 186 210 216 225 241 246 C92334 150 181 209 224 250 263 274 288 C92335 160 175 207 232 240 256 272 286 C92336 149 166 182 202 217 231 236 250 C92337 156 186 213 234 254 260 278 285 C92338 167 188 198 226 233 251 260 260 C92339 160 184 215 239 250 281 290 306 Covance 6329-228 3M T-6316.1 PAGE: 5 WEEK WEEK 910 406 419 470 488 467 482 482 503 441 458 500 525 456 471 462 481 474 489 405 416 413 428 518 541 477 488 552 573 460 480 471 483 477 490 517 535 279 281 249 249 289 306 260 262 296 309 269 276 313 324 4022 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 8 - 0 PPM C92340 159 187 202 223 238 255 268 268 C92341 173 202 230 243 267 277 290 301 C92342 143 179 201 220 233 240 260 258 C92343 150 186 209 232 255 256 279 288 C92344 137 162 186 198 219 235 237 243 C92345 165 190 219 251 276 293 300 C92346 156 180 211 230 244 255 271 281 C92347 142 165 180 198 208 218 223 229 C92348 143 170 181 214 224 224 241 247 C92349 136 160 199 228 241 265 275 292 C92350 154 183 212 238 256 260 269 285 C92351 140 161 180 190 208 216 217 224 C92352 162 198 221 236 C92353 165 194 209 236 252 260 273 274 C92354 154 172 197 212 237 255 261 268 C92355 146 175 195 219 C92356 163 192 200 220 250 265 289 278 C92357 147 174 208 238 258 277 301 317 C92358 159 188 221 234 246 263 265 280 C92359 147 178 193 205 225 241 252 255 C92360 163 204 238 263 271 289 314 319 C92361 156 179 188 207 219 233 238 252 C92362 129 153 162 191 211 223 219 239 C92363 161 194 216 246 261 277 289 292 C92364 137 159 180 188 203 215 219 230 C92365 162 192 204 234 243 252 263 274 C92366 162 181 219 241 C92367 141 169 191 204 221 231 242 249 C92368 158 203 226 266 278 303 303 319 C92369 160 181 199 225 245 263 271 284 C92370 160 177 187 219 236 239 242 258 Covance 6329-228 3M T-6316.1 PAGE: 6 WEEK 9 WEEK 10 287 288 302 316 270 278 292 295 250 254 334 345 285 285 232 241 258 261 310 304 292 292 236 242 282 288 275 283 281 271 327 330 275 288 261 270 330 333 265 264 251 253 304 306 237 240 284 282 256 255 339 344 299 304 270 278 4023 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 8 - 0 PPM C92371 151 180 198 227 237 265 274 279 C92372 156 187 214 252 247 277 293 312 C92373 151 172 186 205 C92374 151 184 206 218 246 260 265 283 C92375 167 195 228 266 271 284 302 320 C92376 140 162 184 199 209 216 223 228 C92377 158 185 222 232 259 277 292 298 C92378 154 181 195 228 243 252 259 272 C92379 133 162 184 203 221 229 240 247 C92380 142 180 205 224 249 253 276 283 C92381 179 209 224 257 272 285 290 300 C92382 146 181 196 205 222 235 245 250 C92383 161 194 223 236 260 277 284 297 C92384 147 169 200 218 232 244 263 273 C92385 169 190 217 234 260 283 279 300 C92386 138 160 181 199 208 226 237 242 C92387 154 179 205 231 236 264 259 273 C92388 152 185 205 227 241 260 268 278 C92389 140 166 190 205 226 240 246 256 C92390 137 139 166 181 194 208 206 226 C92391 175 210 232 264 284 297 310 315 C92392 140 170 187 209 221 226 241 239 C92393 163 201 230 266 263 275 278 297 C92394 168 197 209 237 244 258 267 272 C92395 153 184 207 225 239 253 269 273 C92396 151 171 186 217 231 242 248 265 C92397 138 161 193 221 235 253 262 274 C92398 147 175 205 234 250 259 276 276 C92399 148 177 191 211 227 227 242 244 C92400 151 171 190 222 235 242 252 264 Covance 6329-228 3M T-6316.1 PAGE: 7 WEEK 9 WEEK 10 294 301 315 308 290 299 332 334 227 229 314 318 283 286 253 256 294 311 312 315 268 272 302 320 277 280 305 312 243 255 297 299 278 283 263 275 228 229 330 328 247 250 311 333 278 291 276 286 272 277 287 290 290 295 253 264 276 274 4024 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 9 - 1 PPM C92401 164 189 214 240 250 276 294 305 C92402 147 177 205 221 C92403 163 190 214 245 251 278 285 299 C92404 149 181 207 222 250 262 277 288 C92405 159 177 207 223 234 245 257 263 C92406 150 177 187 214 217 236 242 252 C92407 150 173 209 224 247 270 283 289 C92408 145 171 199 218 233 247 256 258 C92409 162 183 205 224 231 242 253 264 C92410 169 191 219 233 247 260 263 276 C92411 147 182 213 233 248 268 274 280 C92412 150 184 206 227 245 244 268 267 C92413 171 196 211 241 253 270 274 292 C92414 174 198 220 237 261 275 284 288 C92415 155 179 210 240 250 276 280 288 C92416 144 169 194 212 220 240 247 252 C92417 149 176 208 228 252 270 272 277 C92418 154 189 210 235 248 265 278 277 C92419 159 185 213 223 249 260 252 269 C92420 156 185 211 224 254 267 279 285 C92421 165 190 214 231 243 256 265 268 C92422 149 163 196 210 224 231 246 255 C92423 147 175 201 211 238 255 262 261 C92424 157 184 195 220 234 244 243 260 C92425 144 179 203 221 228 244 255 256 C92426 126 158 181 191 211 223 230 237 C92427 155 169 198 205 220 238 241 240 C92428 172 195 208 241 256 272 272 289 C92429 141 166 186 204 217 232 243 248 C92430 157 195 217 234 244 265 280 283 C92431 158 189 213 234 244 264 274 288 Covance 6329-228 3M T-6316.1 PAGE: 8 WEEK 9 WEEK 10 309 328 308 309 287 305 265 265 260 269 302 303 272 277 267 269 281 285 293 303 278 284 301 316 305 303 303 319 250 257 284 299 284 296 276 282 297 304 277 284 260 264 278 285 267 279 263 268 248 251 252 260 298 299 258 262 285 294 288 311 4025 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 9 - 1 PPM C92432 139 164 182 195 210 223 228 232 C92433 136 171 185 218 229 249 254 259 C92434 150 174 187 220 213 231 237 246 C92435 168 212 252 269 C92436 132 159 173 201 207 230 231 245 C92437 155 181 197 211 222 239 249 254 C92438 146 174 199 224 234 239 257 260 C92439 145 173 205 223 238 254 266 264 C92440 150 179 196 215 230 245 250 263 C92441 163 173 212 228 241 255 262 261 C92442 166 195 208 244 262 281 284 317 C92443 164 198 223 246 258 279 294 308 C92444 144 169 184 206 217 230 238 247 C92445 146 179 228 253 274 310 319 339 C92446 155 178 197 223 249 264 272 288 C92447 166 193 226 249 266 279 296 EXCLUDE C92448 171 192 232 235 271 281 291 303 C92449 136 162 179 204 219 229 234 244 C92450 177 209 220 248 268 278 291 302 C92451 154 171 198 209 235 247 258 248 C92452 156 177 203 221 235 246 263 271 C92453 158 184 199 224 238 257 259 266 C92454 164 191 220 243 256 263 279 285 C92455 146 174 193 207 224 245 252 252 C92456 133 158 178 196 205 220 225 230 C92457 149 183 203 229 233 253 269 259 C92458 147 172 195 209 222 240 252 253 C92459 139 160 183 192 209 221 222 233 C92460 130 152 175 183 197 210 223 228 C92461 152 175 201 221 236 243 256 265 C92462 146 166 175 197 217 221 220 232 Covance 6329-228 3M T-6316.1 PAGE: 9 WEEK 9 WEEK 10 240 246 268 271 256 252 244 256 257 271 271 278 278 290 263 275 269 278 325 335 314 314 250 258 348 350 296 299 307 313 298 316 254 258 296 314 254 264 275 277 271 279 289 291 268 271 237 238 267 286 262 265 242 246 228 233 269 272 234 232 4026 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 9 - 1 PPM C92463 170 215 233 275 C92464 128 158 180 202 211 219 230 239 C92465 146 171 205 226 245 259 264 274 C92466 158 183 198 227 241 256 266 258 C92467 156 187 201 233 C92468 153 174 203 218 232 241 240 250 C92469 129 151 181 199 214 216 231 237 C92470 160 189 205 229 Covance 6329-228 3M T-6316.1 PAGE: 10 WEEK 9 WEEK 10 240 248 286 299 274 274 262 266 239 236 4027 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 8 - 0 PPM C92191 428 431 440 425 447 462 472 498 C92193 489 503 511 513 513 526 544 559 C92194 488 506 527 542 551 565 577 609 C92195 442 456 465 475 476 489 494 524 C92197 509 525 526 537 533 551 559 588 C92198 442 C92199 418 434 446 457 454 473 487 513 C92200 460 480 485 496 481 496 512 545 C92201 483 496 500 507 504 521 528 561 C92202 452 467 472 479 478 492 491 516 C92203 540 557 573 575 591 606 621 664 C92204 432 438 445 441 454 469 472 493 C92205 605 627 636 651 661 680 690 733 C92206 501 507 520 531 544 556 568 602 C92208 502 519 530 537 543 549 556 592 C92209 311 285 335 377 418 448 466 498 C92210 521 526 539 553 557 574 571 610 C92211 457 467 476 479 486 491 497 516 C92212 398 403 415 415 423 428 434 455 C92213 482 490 496 499 514 526 537 556 C92214 557 566 579 587 597 611 618 664 C92215 518 531 544 550 566 587 599 643 C92217 502 514 526 533 548 569 573 593 C92218 465 477 499 498 509 520 526 539 C92219 580 598 612 614 635 656 672 710 C92220 520 539 549 558 574 587 595 614 C92221 397 402 417 425 431 441 440 458 C92222 479 501 524 526 531 543 553 575 C92223 465 476 486 491 503 511 520 547 C92224 526 537 555 560 572 578 588 614 C92225 487 501 516 530 538 545 547 588 C92226 480 491 503 506 511 528 534 560 Covance 6329-228 3M T-6316.1 PAGE: 11 WEEK 25 520 576 645 555 611 540 569 579 541 701 508 748 629 626 522 636 537 471 577 682 670 627 545 733 640 469 600 570 632 616 579 WEEK 29 525 583 656 558 608 545 550 585 542 709 513 771 648 655 547 642 545 487 585 685 695 637 564 754 660 480 618 581 637 630 592 4028 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 8 - 0 PPM C92227 503 524 532 544 554 562 579 609 C92228 462 480 497 505 514 521 533 559 C92229 539 552 565 574 581 590 603 636 C92230 510 532 548 558 569 581 589 607 C92231 504 520 528 521 538 539 550 565 C92232 578 602 624 631 639 644 646 678 C92233 469 479 497 501 510 518 526 550 C92234 542 561 575 583 598 606 618 661 C92235 421 431 438 445 449 456 464 470 C92236 424 431 441 452 455 453 460 470 C92237 570 584 597 606 626 625 631 661 C92238 521 540 556 565 571 585 592 626 C92239 516 529 546 552 568 578 597 622 C92240 522 539 556 570 583 599 615 648 C92241 446 455 466 473 494 503 517 533 C92242 534 552 562 569 579 588 594 622 C92243 561 571 587 602 617 633 644 682 C92244 548 566 576 578 598 608 613 643 C92245 562 576 601 618 634 643 658 707 C92246 444 459 478 486 494 501 521 548 C92247 580 602 620 631 640 662 672 713 C92248 564 578 589 599 614 629 637 670 C92249 479 487 496 504 510 527 532 560 C92250 520 546 555 572 585 604 622 665 C92251 499 509 518 529 536 547 550 574 C92252 454 467 479 501 509 520 533 547 C92253 528 548 554 568 586 599 607 642 C92254 553 567 577 596 604 614 629 660 C92255 419 425 437 442 C92256 556 573 581 598 C92257 536 545 537 535 Covance 6329-228 3M T-6316.1 PAGE: 12 WEEK 25 WEEK 29 635 649 578 595 662 689 637 658 568 590 717 760 576 591 704 725 487 496 491 499 690 708 659 681 651 675 678 691 566 586 651 677 721 738 669 689 738 754 574 583 749 754 714 723 577 598 706 723 598 614 570 584 687 707 684 691 4029 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 8 - 0 PPM C92258 527 550 560 582 C92260 501 518 527 534 GROUP: MALE 9 - 1 PPM C92261 448 458 468 472 477 480 489 519 C92262 544 553 571 580 596 617 634 647 C92263 554 570 584 592 607 627 637 669 C92265 533 553 563 570 580 593 603 628 C92266 467 483 492 502 509 514 523 540 C92267 471 496 497 507 506 512 520 539 C92268 623 642 653 665 677 697 711 766 C92269 541 556 573 589 588 605 630 679 C92270 430 444 462 469 474 488 497 528 C92271 587 601 619 632 644 653 664 713 C92272 443 459 461 472 488 494 497 528 C92273 531 545 559 569 578 588 602 639 C92274 487 506 518 520 526 538 538 558 C92275 439 452 463 464 473 483 487 508 C92276 479 491 509 513 525 531 543 568 C92277 562 582 596 606 624 635 642 680 C92278 494 506 509 518 527 547 553 572 C92279 492 513 512 531 542 551 561 589 C92281 493 504 512 515 525 529 532 549 C92282 492 502 515 523 530 528 536 565 C92283 477 489 507 512 524 533 540 561 C92285 588 612 620 628 647 670 683 730 C92286 522 544 559 571 579 593 614 657 C92287 513 524 539 544 554 558 566 595 C92288 538 554 574 583 598 606 625 679 C92289 502 517 524 528 541 544 549 581 Covance 6329-228 3M T-6316.1 PAGE: 13 WEEK 25 WEEK 29 532 548 652 657 704 705 646 647 565 576 565 562 802 822 711 747 560 562 744 753 545 539 672 690 581 590 533 544 580 594 695 719 599 611 616 636 560 576 580 592 589 610 768 811 686 706 624 634 705 716 602 611 4030 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 9 - 1 PPM C92290 478 494 508 526 539 550 562 589 C92291 453 458 467 473 482 485 489 509 C92292 421 442 458 466 477 484 491 529 C92293 499 506 525 533 547 561 568 606 C92294 501 518 529 529 548 555 562 586 C92295 535 549 555 562 566 582 585 611 C92296 474 484 498 513 525 537 541 579 C92297 417 422 434 438 450 449 447 474 C92298 588 602 619 628 643 654 666 685 C92299 434 450 455 471 470 476 483 500 C92301 518 527 549 556 562 574 581 599 C92302 623 643 666 674 682 693 703 745 C92303 498 517 525 526 538 545 554 585 C92304 548 559 575 581 593 601 605 645 C92305 527 542 555 565 586 595 600 618 C92306 491 513 534 541 551 560 569 610 C92307 486 505 515 522 538 549 556 582 C92308 436 432 431 438 443 448 451 450 C92309 470 480 490 487 499 504 507 532 C92310 562 574 589 596 603 630 639 677 C92311 527 540 554 560 566 591 597 638 C92312 425 433 438 450 454 459 466 484 C92313 503 517 527 533 551 568 586 613 C92314 493 512 522 521 525 535 546 572 C92315 513 530 537 549 557 573 584 615 C92316 462 475 481 487 489 502 504 521 C92317 545 558 567 573 593 616 623 666 C92318 483 499 516 528 534 545 558 577 C92319 491 506 515 520 528 537 530 561 C92320 500 520 529 540 547 552 562 590 C92321 424 435 440 447 449 457 466 480 Covance 6329-228 3M T-6316.1 PAGE: 14 WEEK 25 WEEK 29 612 626 521 523 567 589 635 647 608 615 629 638 612 622 488 498 718 730 523 534 629 647 786 793 613 624 664 670 637 647 653 673 604 631 466 485 545 553 699 709 669 695 492 504 639 667 591 601 640 657 533 541 692 717 608 624 568 588 608 630 498 508 4031 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: MALE 9 - 1 PPM C92322 434 440 452 457 464 474 473 499 C92323 556 573 585 587 595 606 623 659 C92324 505 515 529 536 553 560 571 593 C92325 580 603 622 637 C92326 489 494 520 522 C92327 495 511 516 519 C92328 506 512 524 525 C92330 555 574 584 595 GROUP: FEMALE 8 - 0 PPM C92331 272 286 295 294 282 296 302 312 C92333 255 268 265 264 271 271 276 282 C92334 304 314 329 326 330 329 333 357 C92336 258 271 272 278 267 272 281 318 C92337 306 302 316 323 317 330 337 352 C92338 277 277 292 297 290 291 299 314 C92339 322 339 353 365 366 369 390 405 C92340 292 303 307 309 313 315 330 348 C92341 315 324 333 346 341 352 365 399 C92342 270 280 288 292 291 304 309 290 C92343 293 305 319 327 336 347 353 377 C92344 253 261 260 273 269 282 276 286 C92345 341 357 361 366 372 387 395 431 C92346 296 303 301 301 305 316 316 329 C92347 250 244 249 254 266 266 262 278 C92348 259 272 270 274 286 290 314 292 C92349 324 322 322 342 333 339 353 362 C92350 292 302 310 304 306 310 315 328 C92351 241 242 254 258 257 251 264 269 C92353 286 297 297 309 307 316 320 329 Covance 6329-228 3M T-6316.1 PAGE: 15 WEEK 25 WEEK 29 521 537 673 688 614 627 325 327 295 295 368 368 338 350 375 389 324 318 425 447 357 383 416 408 298 306 399 420 306 308 487 421 331 344 289 292 290 299 375 367 332 331 276 279 333 335 4032 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 8 - 0 PPM C92354 282 294 298 300 301 311 315 321 C92356 285 293 288 303 308 303 318 320 C92357 344 359 363 369 384 393 396 422 C92358 286 291 295 299 290 304 302 304 C92359 275 278 295 304 298 300 311 315 C92360 332 348 351 339 338 343 348 361 C92361 268 275 284 291 290 295 299 288 C92362 248 262 271 275 270 275 281 289 C92363 307 322 317 331 326 339 342 359 C92364 238 245 249 254 247 259 258 262 C92365 281 291 307 302 300 311 320 334 C92367 262 269 274 272 276 287 291 300 C92368 349 360 354 373 372 368 360 389 C92369 306 319 318 321 325 332 342 361 C92370 264 286 298 295 286 299 305 322 C92371 298 300 309 316 319 320 328 347 C92372 315 313 330 330 331 339 331 350 C92374 294 314 316 316 311 312 312 339 C92375 341 354 360 366 368 384 383 409 C92376 232 240 238 245 241 247 248 251 C92377 329 324 324 331 340 342 356 359 C92378 286 297 299 305 295 308 303 337 C92379 250 260 258 264 263 265 268 288 C92380 304 324 329 335 341 341 355 346 C92381 312 324 334 333 336 339 340 371 C92382 265 276 284 287 284 296 303 311 C92383 316 327 334 338 332 338 350 353 C92384 286 296 295 295 297 302 305 316 C92385 322 323 342 338 352 360 355 380 C92386 260 268 266 273 279 282 279 292 C92387 311 316 328 335 326 344 347 377 Covance 6329-228 3M T-6316.1 PAGE: 16 WEEK 25 WEEK 29 331 350 328 335 441 473 309 312 321 325 373 388 323 314 284 301 365 369 276 290 330 345 317 315 405 418 371 384 331 359 351 385 363 368 356 378 438 448 270 269 362 357 354 368 297 298 360 381 391 404 322 333 369 373 324 325 412 447 301 324 420 435 4033 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 8 - 0 PPM C92388 280 293 303 303 309 321 322 336 C92389 273 277 280 287 281 290 293 300 C92390 241 240 259 260 256 263 265 283 C92391 327 334 332 342 346 354 358 373 C92392 247 251 263 259 257 267 261 274 C92393 313 318 321 336 345 363 345 355 C92394 294 294 294 301 307 309 305 314 C92395 285 292 291 297 296 303 308 316 C92396 276 279 283 293 C92397 283 289 292 302 C92398 288 303 315 313 C92399 255 263 259 266 C92400 276 289 299 301 GROUP: FEMALE 9 - 1 PPM C92401 328 340 344 354 353 361 361 379 C92403 320 331 332 335 340 352 355 381 C92404 299 310 317 321 317 322 328 357 C92405 269 275 276 275 279 284 280 294 C92406 274 261 282 270 277 272 286 290 C92407 304 310 324 325 311 318 325 355 C92408 274 277 284 288 284 286 288 307 C92409 270 279 284 279 284 293 289 303 C92410 287 283 299 296 292 299 300 309 C92411 302 303 314 312 310 327 337 343 C92412 275 289 288 284 297 288 297 315 C92413 310 320 315 335 357 366 379 406 C92414 314 317 319 329 330 334 335 354 C92415 314 322 330 325 341 337 344 359 C92416 265 261 262 265 271 277 273 266 Covance 6329-228 3M T-6316.1 PAGE: 17 WEEK 25 WEEK 29 344 349 313 324 301 307 382 390 272 278 361 404 328 343 330 333 407 409 423 442 372 374 299 312 297 291 378 392 317 315 313 313 328 316 333 346 310 310 451 480 372 381 372 380 297 290 4034 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 9 - 1 PPM C92417 296 297 312 315 312 313 318 322 C92418 292 302 306 316 312 317 315 309 C92419 278 289 294 295 297 299 299 285 C92420 304 306 314 321 321 333 339 348 C92421 285 285 297 303 299 307 304 309 C92422 265 273 279 277 283 293 289 302 C92423 291 287 295 301 302 299 305 315 C92424 276 285 295 298 292 299 298 328 C92425 277 276 276 286 295 295 296 307 C92426 249 247 259 266 263 262 266 277 C92427 260 254 265 272 272 269 280 284 C92428 297 301 297 315 313 315 322 340 C92429 263 268 276 279 281 281 283 303 C92430 297 296 296 307 307 313 308 324 C92431 321 321 317 332 343 351 335 343 C92432 248 243 257 255 264 263 262 283 C92433 280 274 279 282 278 280 283 298 C92434 261 260 261 269 267 281 274 282 C92436 250 267 266 278 273 285 288 295 C92437 265 272 268 288 289 293 287 315 C92438 273 279 283 282 287 286 292 302 C92439 288 285 301 305 304 308 314 329 C92440 283 276 276 289 285 297 298 305 C92441 275 277 284 289 286 291 295 321 C92442 337 343 350 360 373 365 380 396 C92443 329 326 335 345 339 353 343 366 C92444 254 251 256 259 265 264 264 274 C92445 371 369 378 394 401 416 419 453 C92446 311 312 326 330 319 332 343 362 C92447 325 330 328 342 335 351 360 408 C92448 315 316 329 343 355 363 371 397 Covance 6329-228 3M T-6316.1 PAGE: 18 WEEK 25 WEEK 29 333 350 356 367 308 317 366 365 336 339 317 339 322 339 339 354 334 333 290 294 293 293 356 365 308 316 339 336 364 363 300 301 299 308 290 296 304 318 328 338 310 317 325 331 309 322 344 360 442 426 384 414 282 278 493 534 363 377 431 448 418 434 4035 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 17 WEEK 21 GROUP: FEMALE 9 - 1 PPM C92449 267 267 280 282 280 292 296 313 C92450 306 312 323 336 341 346 353 382 C92451 273 283 286 290 295 291 300 341 C92452 286 293 294 294 303 305 305 321 C92453 282 296 309 312 312 317 324 334 C92454 294 300 310 308 316 317 318 321 C92455 284 296 291 288 296 299 297 309 C92456 250 253 255 253 257 265 261 271 C92457 289 295 295 302 304 303 302 322 C92458 277 273 287 290 284 289 300 336 C92459 245 245 257 252 256 264 260 282 C92460 244 250 258 265 267 273 266 291 C92461 274 284 284 285 293 296 295 312 C92462 232 242 249 253 252 258 264 268 C92464 252 251 258 263 C92465 295 295 308 314 C92466 282 286 288 298 C92468 265 278 279 277 C92469 244 250 247 250 Covance 6329-228 3M T-6316.1 PAGE: 19 WEEK 25 WEEK 29 317 319 398 412 359 366 322 330 336 354 328 344 320 323 280 284 321 323 363 388 286 289 297 308 333 336 276 286 4036 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 8 - 0 PPM C92191 547 578 578 609 616 636 652 661 C92193 605 623 624 636 633 648 649 661 C92194 686 731 737 761 774 779 796 816 C92195 584 622 630 654 661 672 C92197 629 642 653 667 673 674 677 667 C92199 569 590 609 635 656 666 C92200 596 634 656 679 701 728 753 767 C92201 606 647 642 661 668 678 693 700 C92202 559 602 624 644 647 657 664 676 C92203 740 803 828 860 888 911 943 977 C92204 520 549 557 568 578 584 599 614 C92205 790 805 833 849 863 882 C92206 680 692 711 740 752 768 772 788 C92208 654 708 734 777 807 847 869 889 C92209 565 601 602 626 644 666 673 695 C92210 659 674 693 727 742 769 782 803 C92211 568 589 608 628 644 657 C92212 501 524 548 568 586 603 616 634 C92213 602 624 628 592 595 C92214 713 746 740 767 758 782 798 823 C92215 709 743 753 791 802 812 817 772 C92217 657 677 695 705 717 733 740 761 C92218 583 582 585 592 600 613 630 647 C92219 788 820 840 878 904 923 940 966 C92220 677 696 705 718 712 721 715 717 C92221 469 483 495 513 523 524 542 542 C92222 637 659 691 709 717 733 751 767 C92223 599 627 642 654 670 681 C92224 638 662 674 697 705 724 724 740 C92225 655 691 709 732 741 758 763 773 C92226 616 646 663 680 711 723 708 734 C92227 670 714 730 759 757 781 798 820 Covance 6329-228 3M T-6316.1 PAGE: 20 WEEK 65 675 657 834 667 789 715 685 995 621 778 911 713 810 654 841 740 779 656 982 699 545 782 746 767 750 849 WEEK 69 680 660 846 645 809 719 687 1016 629 762 936 724 825 644 828 674 814 676 970 685 546 786 737 599 761 860 4037 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 8 - 0 PPM C92228 619 646 663 691 708 705 680 561 C92229 729 767 783 805 839 862 879 898 C92230 668 697 715 734 748 764 C92231 608 638 642 666 681 689 715 733 C92232 790 816 848 897 940 968 988 1009 C92233 608 632 641 670 679 692 692 707 C92234 742 794 829 874 877 941 954 1000 C92235 509 527 542 561 554 583 582 592 C92236 513 524 535 560 571 588 589 606 C92237 735 773 792 834 866 887 913 934 C92238 711 735 761 784 797 819 C92239 686 704 721 743 746 757 761 769 C92240 720 747 773 795 801 826 825 846 C92241 616 655 682 712 736 755 778 816 C92242 701 725 755 782 808 831 831 806 C92243 770 808 828 842 851 869 865 878 C92244 725 758 775 811 818 842 841 862 C92245 779 817 827 847 839 855 857 897 C92246 600 618 635 669 684 710 C92247 793 832 850 873 908 932 C92248 752 774 796 817 824 835 813 771 C92249 622 652 663 684 700 727 708 742 C92250 757 797 823 854 870 888 930 951 C92251 643 668 691 719 724 747 748 773 C92252 593 617 604 609 620 613 626 631 C92253 687 748 772 769 777 791 C92254 712 723 737 751 762 770 785 800 Covance 6329-228 3M T-6316.1 PAGE: 21 WEEK 65 WEEK 69 921 751 1024 715 1009 613 610 946 775 869 830 743 842 881 712 651 727 970 789 642 814 932 759 1019 727 1048 617 621 977 790 883 845 684 447 701 980 788 651 824 4038 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 9 - 1 PPM C92261 569 581 599 625 638 658 680 696 C92262 675 696 705 725 741 746 755 768 C92263 733 756 767 789 814 832 840 858 C92265 679 671 704 684 722 735 753 765 C92266 601 614 638 664 687 699 717 744 C92267 566 581 599 619 634 653 C92268 872 917 944 986 1020 1046 1081 1120 C92269 790 827 858 888 911 940 967 989 C92270 588 617 633 643 669 686 702 720 C92271 782 814 841 871 889 908 930 953 C92272 560 590 612 623 645 667 685 704 C92273 735 773 787 C92274 610 623 646 656 681 689 C92275 569 598 607 616 640 641 639 658 C92276 606 623 647 653 654 623 624 618 C92277 746 776 790 810 816 823 799 C92278 631 654 671 688 713 720 745 754 C92279 657 678 704 723 727 747 769 787 C92281 605 615 631 646 677 680 696 716 C92282 613 638 659 669 670 683 690 702 C92283 629 648 668 683 702 722 751 759 C92285 861 898 932 962 995 1027 1045 1087 C92286 726 744 757 774 793 797 C92287 662 685 704 725 746 752 C92288 745 775 808 834 855 889 C92289 637 650 672 687 706 716 646 709 C92290 648 668 678 691 693 691 611 484 C92291 534 556 571 577 583 597 590 616 C92292 630 664 695 726 741 755 711 597 C92293 675 692 689 C92294 645 648 655 686 701 710 725 751 Covance 6329-228 3M T-6316.1 PAGE: 22 WEEK 65 WEEK 69 715 767 873 757 755 1150 999 723 958 730 656 612 768 799 729 705 769 1075 734 629 490 752 730 771 874 749 768 1170 1031 747 963 735 660 584 771 804 741 674 775 1017 744 630 701 4039 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: MALE 9 - 1 PPM C92295 674 697 710 724 739 750 761 777 C92296 650 665 677 698 718 721 732 733 C92297 514 541 543 558 571 580 588 590 C92298 750 752 761 781 802 821 830 828 C92299 534 550 562 568 564 581 593 611 C92301 677 696 709 741 767 773 784 808 C92302 838 873 900 924 949 987 1002 1025 C92303 644 665 675 689 706 715 C92304 691 710 741 759 781 797 821 843 C92305 679 696 719 739 765 759 739 587 C92306 689 716 735 753 782 786 793 815 C92307 653 657 693 715 727 717 738 726 C92308 494 497 509 510 513 520 535 539 C92309 578 593 607 615 643 657 672 693 C92310 728 754 774 778 798 811 807 820 C92311 730 733 777 805 840 855 877 911 C92312 515 517 522 521 508 502 488 480 C92313 695 711 730 755 774 792 798 817 C92314 628 645 663 676 683 695 699 708 C92315 680 691 712 731 746 755 C92316 559 580 599 611 628 634 645 659 C92317 751 772 815 828 841 868 C92318 649 663 675 705 729 746 766 787 C92319 585 599 621 632 646 658 666 680 C92320 661 676 691 722 739 764 C92321 530 542 563 571 576 595 606 623 C92322 565 580 600 625 641 652 661 681 C92323 702 713 737 768 792 804 832 860 C92324 650 672 689 702 697 704 Covance 6329-228 3M T-6316.1 PAGE: 23 WEEK 65 WEEK 69 782 723 605 706 616 818 1098 846 822 731 558 684 832 924 462 824 725 663 797 689 614 697 874 790 712 613 625 834 1047 854 842 747 568 695 845 938 835 736 666 810 683 621 708 898 4040 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 8 - 0 PPM C92331 343 360 374 383 403 426 437 451 C92333 311 337 344 365 354 400 415 421 C92334 401 425 440 437 475 474 510 539 C92336 365 376 390 408 418 431 456 470 C92337 410 431 463 487 503 527 533 556 C92338 352 371 381 409 418 437 447 478 C92339 458 495 507 548 573 595 608 641 C92340 408 435 455 474 501 520 558 590 C92341 444 478 489 512 525 543 C92342 323 319 325 328 327 C92343 449 456 462 466 465 445 420 C92344 309 334 346 353 367 381 391 409 C92346 352 353 382 394 418 412 337 326 C92347 309 318 344 317 C92348 304 310 303 308 307 324 336 346 C92349 385 413 424 439 442 458 468 488 C92350 348 366 373 383 390 405 419 438 C92351 294 299 307 334 310 333 355 347 C92353 343 359 374 388 406 420 425 450 C92354 364 373 384 393 405 412 438 467 C92356 347 366 368 373 386 400 C92357 510 548 581 615 622 640 C92358 320 330 329 340 345 349 370 375 C92359 328 347 353 368 378 391 409 426 C92360 403 417 419 431 444 458 471 478 C92361 327 339 366 370 385 400 411 436 C92362 325 323 324 334 344 351 368 365 C92363 403 424 442 459 473 472 466 479 C92364 304 329 335 356 362 315 306 223 C92365 355 367 364 385 399 405 C92367 319 326 341 351 356 370 364 368 Covance 6329-228 3M T-6316.1 PAGE: 24 WEEK 65 WEEK 69 462 478 442 451 559 583 478 473 577 577 396 373 648 661 608 621 427 444 353 361 485 504 443 451 356 371 457 466 469 486 378 390 435 447 507 515 446 456 380 387 500 521 389 401 4041 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 8 - 0 PPM C92368 433 456 471 473 481 509 521 535 C92369 407 436 447 463 486 505 508 537 C92370 382 402 414 432 465 476 510 534 C92371 402 419 435 446 467 488 484 495 C92372 365 378 396 412 431 447 C92374 421 436 444 465 476 475 487 505 C92375 470 503 528 558 597 624 652 695 C92376 275 284 302 316 323 347 C92377 367 390 402 403 436 458 C92378 388 404 414 430 446 474 474 485 C92379 318 329 340 344 358 372 373 380 C92380 407 429 448 456 495 500 519 538 C92381 424 456 465 469 491 496 513 533 C92382 353 376 385 376 394 397 232 C92383 377 390 407 414 429 438 446 468 C92384 340 351 365 391 404 415 428 452 C92385 475 506 532 566 596 607 638 668 C92386 339 364 375 396 404 410 403 431 C92387 461 474 487 496 508 530 544 551 C92388 376 376 404 419 430 461 C92389 332 348 356 361 376 380 C92390 317 335 341 356 356 370 C92391 418 464 493 514 533 553 559 567 C92392 280 297 310 318 323 273 320 378 C92393 450 476 492 508 538 539 549 587 C92394 365 383 397 419 426 451 455 481 C92395 352 368 383 390 413 418 Covance 6329-228 3M T-6316.1 PAGE: 25 WEEK 65 WEEK 69 543 558 550 554 564 586 503 521 520 524 717 752 493 459 280 321 552 558 478 316 478 489 462 474 670 669 426 411 568 579 569 609 608 615 492 515 4042 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 9 - 1 PPM C92401 442 471 485 514 536 557 588 618 C92403 462 488 501 524 466 C92404 396 421 449 462 485 490 519 530 C92405 333 347 359 369 370 388 408 414 C92406 291 314 331 329 338 358 C92407 431 421 467 489 505 516 C92408 323 341 337 351 350 376 388 407 C92409 328 350 370 386 405 409 424 435 C92410 333 351 354 359 366 380 398 405 C92411 368 378 392 390 407 429 C92412 327 345 352 374 384 385 407 418 C92413 489 499 494 498 494 489 504 550 C92414 399 424 433 446 466 472 487 509 C92415 398 415 429 449 478 487 517 539 C92416 298 323 334 347 363 373 372 384 C92417 376 402 408 423 437 463 479 520 C92418 375 395 407 419 444 456 C92419 328 332 333 335 342 339 350 361 C92420 391 403 418 437 461 464 487 517 C92421 353 367 375 384 401 382 409 439 C92422 352 369 384 384 414 426 C92423 351 366 387 395 409 428 438 458 C92424 367 385 393 397 423 427 C92425 342 379 389 394 405 422 436 460 C92426 303 311 318 320 322 338 334 343 C92427 307 332 351 392 437 461 478 543 C92428 359 386 385 403 402 411 434 444 C92429 335 342 352 373 385 393 376 383 C92430 341 361 367 385 404 402 421 438 C92431 377 408 414 433 457 475 502 502 C92432 311 328 339 349 366 387 390 411 Covance 6329-228 3M T-6316.1 PAGE: 26 WEEK 65 WEEK 69 633 659 551 564 437 431 407 422 444 455 411 407 429 439 550 580 557 546 384 401 527 537 376 365 536 538 455 473 459 469 468 473 352 356 457 461 549 554 422 408 4043 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 33 WEEK 37 WEEK 41 WEEK 45 WEEK 49 WEEK 53 WEEK 57 WEEK 61 GROUP: FEMALE 9 - 1 PPM C92433 316 329 341 353 375 389 C92434 302 317 329 336 350 365 381 392 C92436 312 317 323 332 338 344 339 351 C92437 358 374 381 382 355 C92438 333 335 347 355 368 369 C92439 333 348 345 370 368 376 386 387 C92440 330 336 354 356 396 391 395 418 C92441 381 394 407 442 470 486 C92442 468 496 528 560 586 606 634 656 C92443 444 469 486 517 555 573 589 620 C92444 297 305 316 330 342 361 353 385 C92445 573 602 645 676 716 732 793 843 C92446 399 410 443 480 493 517 550 594 C92447 467 487 500 506 545 563 592 637 C92448 447 468 465 496 506 522 533 545 C92449 310 329 333 346 370 386 C92450 434 451 458 471 471 487 505 524 C92451 380 393 420 436 447 466 477 503 C92452 342 340 357 370 381 396 395 408 C92453 370 390 404 424 439 456 486 492 C92454 351 371 389 411 446 450 476 507 C92455 339 353 363 383 396 420 427 453 C92456 289 302 316 335 354 383 408 427 C92457 342 361 365 375 384 393 398 420 C92458 411 435 452 469 486 504 521 534 C92459 504 322 305 321 336 351 372 377 C92460 317 341 339 346 372 384 397 409 C92461 360 376 393 400 425 446 477 414 C92462 279 295 326 358 393 400 435 449 Covance 6329-228 3M T-6316.1 PAGE: 27 WEEK 65 WEEK 69 400 418 367 366 395 409 421 419 593 660 660 375 394 875 878 610 606 659 653 476 394 536 538 521 537 425 437 512 527 481 485 468 463 445 450 421 430 350 388 400 416 416 465 485 4044 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: C92191 C92193 C92194 C92197 C92200 C92201 C92202 C92203 C92204 C92206 C92208 C92209 C92210 C92212 C92214 C92215 C92217 C92218 C92219 C92220 C92221 C92222 C92224 C92226 C92227 C92229 C92231 C92232 C92233 C92234 C92235 C92236 MALE 8 - 0 PPM 695 694 670 679 855 843 649 633 823 682 726 715 621 607 1041 1015 655 644 657 916 938 737 737 843 839 669 666 786 736 610 907 1052 686 677 1009 1022 626 483 545 536 792 768 753 757 766 790 900 906 950 932 770 772 1063 1060 729 743 1063 1074 628 606 637 635 705 689 872 616 741 585 1020 603 967 724 851 670 705 693 1061 531 800 748 800 933 839 803 1078 759 953 625 649 733 714 890 634 792 565 1023 1003 728 861 699 607 697 1058 522 825 767 748 950 695 835 1080 773 685 649 648 733 714 902 608 793 1002 1014 734 867 709 689 474 811 758 611 965 832 1054 768 630 540 767 725 907 619 800 957 1043 726 906 698 665 348 842 789 976 849 1016 763 622 807 739 922 607 857 1079 642 771 654 614 866 817 844 866 858 734 636 828 703 854 576 730 1049 515 592 483 843 795 708 837 525 Covance 6329-228 3M T-6316.1 PAGE: 28 WEEK 105 696 565 580 1078 565 417 856 843 401 4045 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 8 - 0 PPM C92237 987 987 1008 1036 1035 957 879 661 C92239 794 787 804 830 832 839 801 650 C92240 893 879 856 C92241 856 853 856 878 857 673 C92250 1005 1016 1062 1063 1028 C92251 812 825 874 C92252 659 655 680 688 708 707 734 718 C92254 839 796 731 483 GROUP: MALE 9 - 1 PPM C92261 748 748 782 791 782 800 828 800 C92262 783 770 769 764 674 614 532 506 C92263 886 881 884 897 885 905 894 840 C92265 724 669 C92266 770 776 797 822 826 841 865 838 C92268 1201 1191 1201 1181 1161 1029 841 C92269 1037 C92270 761 766 802 830 854 876 886 818 C92271 946 928 900 874 811 C92272 733 722 705 680 C92275 656 654 647 661 652 634 593 483 C92276 371 C92278 774 763 785 786 806 827 836 820 C92279 819 808 763 665 542 C92281 750 731 759 764 766 757 749 714 C92282 652 628 638 647 639 638 641 614 C92283 781 781 814 839 835 850 831 772 C92285 829 C92289 745 741 773 769 752 735 701 627 C92291 634 635 657 677 676 695 Covance 6329-228 3M T-6316.1 PAGE: 29 WEEK 105 504 712 823 791 836 797 836 711 613 712 559 4046 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: MALE 9 - 1 PPM C92294 C92295 C92296 C92297 C92299 C92301 C92302 C92304 C92306 C92307 C92308 C92309 C92310 C92311 C92313 C92314 C92316 C92318 C92319 C92321 C92322 C92323 657 809 642 628 635 845 1059 873 853 750 573 712 826 936 844 732 679 817 690 632 732 921 700 808 641 641 837 1058 866 844 707 571 709 938 852 743 690 821 676 626 727 938 GROUP: FEMALE 8 - 0 PPM C92331 C92333 C92334 C92336 C92337 C92339 514 465 590 486 546 681 525 485 608 473 509 701 755 823 650 647 876 1072 871 861 707 570 728 959 856 759 690 825 702 629 751 950 525 502 650 420 445 714 653 849 678 653 892 1069 899 887 678 564 738 981 876 763 633 825 702 625 760 948 495 536 677 757 548 504 377 864 888 891 859 686 700 705 687 665 677 693 666 046 929 896 896 886 914 942 888 626 612 592 522 559 557 594 600 740 749 743 678 994 1001 1039 1037 899 918 899 673 550 654 640 559 528 816 818 849 842 693 723 708 693 625 637 645 618 771 793 811 788 955 970 985 939 473 549 564 598 597 672 699 710 703 767 743 Covance 6329-228 3M T-6316.1 PAGE: 30 WEEK 105 861 677 608 555 594 1044 825 690 606 799 850 608 705 4047 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 8 - 0 PPM C92340 614 573 C92344 450 472 470 493 479 487 481 421 C92348 374 378 387 399 408 427 438 432 C92349 523 514 C92350 452 472 472 459 434 401 C92351 382 386 385 408 426 435 455 443 C92353 479 497 512 547 542 428 C92354 496 520 541 560 557 540 518 473 C92358 402 404 407 440 437 444 444 410 C92359 448 463 468 472 443 326 C92360 518 544 533 538 583 599 614 604 C92361 470 481 498 505 525 571 634 672 C92362 384 396 395 401 393 382 383 371 C92363 547 572 587 C92367 408 423 435 453 459 464 478 481 C92368 576 582 595 649 660 660 640 529 C92369 553 554 558 575 562 560 572 590 C92370 622 653 658 692 695 679 655 C92371 540 545 548 562 521 C92374 522 542 560 589 598 607 626 604 C92375 762 763 696 562 C92378 315 C92380 467 434 339 C92383 502 509 527 549 564 574 599 584 C92384 480 464 488 473 C92385 678 661 647 536 504 429 C92386 417 418 403 401 402 387 375 350 C92387 416 C92391 676 703 698 667 623 521 C92393 630 638 594 616 545 509 C92394 516 415 372 382 Covance 6329-228 3M T-6316.1 PAGE: 31 WEEK 105 464 452 414 381 630 340 498 658 609 596 337 4048 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 9 - 1 PPM C92401 688 685 640 565 474 C92404 572 578 519 C92405 454 470 480 487 493 497 391 C92408 433 441 432 440 466 461 456 471 C92409 475 487 496 514 531 534 429 C92410 423 428 442 455 466 479 497 496 C92412 448 448 465 466 483 496 505 494 C92413 619 629 635 666 678 631 625 519 C92415 510 501 516 551 531 C92416 407 423 429 439 445 448 432 439 C92417 569 602 611 530 403 275 C92419 389 387 402 415 428 434 449 446 C92420 572 C92421 483 496 503 524 544 565 577 541 C92423 482 492 500 511 533 541 572 584 C92425 494 516 525 565 593 606 634 C92426 346 343 352 361 362 350 378 384 C92430 497 482 501 519 533 552 555 542 C92431 564 562 584 611 619 637 651 644 C92432 356 345 C92434 431 430 436 454 492 C92436 381 382 381 415 428 446 C92439 424 413 426 433 435 450 461 460 C92440 448 440 C92443 680 707 744 783 795 808 802 718 C92444 414 417 431 419 431 447 450 453 C92445 877 888 903 938 950 950 C92446 619 603 433 C92447 615 518 436 392 338 C92450 531 488 405 357 281 C92451 557 566 574 597 604 549 526 534 Covance 6329-228 3M T-6316.1 PAGE: 32 WEEK 105 487 497 452 442 466 611 414 542 640 474 620 457 547 4049 Covance 6329-212 Appendix 3 Individual Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 73 WEEK 77 WEEK 81 WEEK 85 WEEK 89 WEEK 93 WEEK 97 WEEK 101 GROUP: FEMALE 9 - 1 PPM C92452 437 462 465 488 481 504 511 511 C92453 545 580 598 615 608 508 445 C92454 456 389 299 C92455 482 498 520 566 580 609 633 524 C92456 468 473 490 513 515 544 551 537 C92457 448 447 413 338 C92459 419 430 437 446 429 400 375 C92460 436 444 447 421 379 360 319 C92462 495 513 527 540 538 457 366 Covance 6329-228 3M T-6316.1 PAGE: 33 WEEK 105 519 561 4050 APPENDIX 4 Covance 6329-212 Covance 6329-228 3M T-6316.1 In d iv id u a l F o o d C o n su m p tio n D a ta (g) In d iv id u a l T est M a terial C o n su m p tio n D a ta (m g /k g /d ay ) 4051 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 8 - 0 PPM C92191 C92192 C92193 C92194 C92195 C92196 C92197 C92198 C92199 C92200 C92201 C92202 C92203 C92204 C92205 C92206 C92207 C92208 C92209 C92210 C92211 C92212 C92213 C92214 C92215 C92216 C92217 C92218 C92219 C92220 C92221 159 162 166 161 156 149 167 173 161 168 181 NOT TAKEN 163 166 173 180 180 179 188 183 174 174 185 177 176 173 173 180 162 201 168 203 177 180 176 174 173 168 170 NOT TAKEN 162 166 179 184 179 167 180 176 161 166 174 167 177 177 168 149 135 143 155 158 164 163 163 162 155 167 183 183 182 186 185 186 158 161 168 177 187 179 173 182 165 163 163 163 162 169 175 164 168 178 193 195 197 200 206 203 145 145 154 159 154 158 157 156 174 187 194 205 205 212 213 203 157 169 174 172 184 183 184 188 181 180 182 NOT TAKEN 158 168 172 171 178 187 178 171 146 147 156 170 166 168 180 175 189 199 212 205 211 213 222 213 153 SPILLED 166 166 166 168 170 163 144 145 146 148 147 147 152 152 161 182 182 186 186 187 195 SPILLED 179 189 198 197 201 196 205 205 164 167 177 184 192 200 197 188 154 152 164 NOT TAKEN 176 184 192 188 SPILLED SPILLED 195 185 152 162 169 170 175 160 161 162 186 207 211 217 218 217 223 227 181 202 181 199 191 183 188 192 146 153 159 158 158 152 156 147 Covance 6329-228 3M T-6316.1 PAGE: 1 WEEK WEEK 910 169 166 177 180 176 187 171 172 177 168 176 156 165 166 205 198 182 174 166 166 203 198 158 158 210 203 193 199 172 170 148 18 211 189 163 169 155 157 197 SPILLED 207 204 198 193 190 199 168 177 226 227 196 208 154 161 4052 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 8 - 0 PPM C92222 C92223 C92224 C92225 C92226 C92227 C92228 C92229 C92230 C92231 C92232 C92233 C92234 C92235 C92236 C92237 C92238 C92239 C92240 C92241 C92242 C92243 C92244 C92245 C92246 C92247 C92248 C92249 C92250 C92251 C92252 188 168 178 154 150 170 159 167 158 172 197 159 182 154 158 184 171 168 181 149 181 176 166 183 149 186 169 172 174 172 163 175 187 191 195 193 197 191 172 177 180 200 180 199 199 178 187 195 200 199 202 195 161 171 175 179 183 180 177 153 161 167 169 171 178 170 169 177 184 181 177 178 178 162 164 168 165 169 171 168 183 194 204 206 201 209 200 171 194 199 221 236 234 SPILLED 176 183 179 179 182 177 171 201 203 223 221 223 220 230 162 168 174 176 184 186 186 195 211 208 212 202 204 211 152 149 149 156 160 165 171 157 160 164 166 163 168 166 186 194 194 194 204 205 202 167 174 175 174 173 180 173 170 176 176 178 190 186 189 187 193 194 191 206 224 215 155 167 171 179 179 171 166 183 191 186 189 184 189 190 185 191 186 188 195 197 199 172 185 189 193 200 208 203 193 198 195 196 197 210 217 157 164 154 156 157 163 178 192 198 194 194 210 206 207 174 189 193 201 202 205 199 180 180 179 205 SPILLED 206 203 178 186 194 195 195 201 188 182 185 183 180 182 182 186 172 SPILLED SPILLED SPILLED SPILLED 192 222 Covance 6329-228 3M T-6316.1 PAGE: 2 WEEK WEEK 910 195 201 191 181 169 178 175 203 SPILLED 170 237 197 210 173 169 197 179 195 199 169 184 207 210 211 185 205 200 196 192 183 SPILLED 189 190 190 183 173 178 177 204 SPILLED 177 245 188 217 177 159 201 182 189 210 169 187 206 202 214 190 210 200 180 199 170 226 4053 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 8 - 0 PPM C92253 183 177 185 189 182 200 205 210 C92254 180 185 203 203 204 204 215 210 C92255 174 182 185 172 171 172 176 187 C92256 167 175 191 184 193 187 196 199 C92257 170 177 185 183 185 187 187 192 C92258 180 187 185 195 192 189 193 188 C92259 177 175 187 NOT TAKEN C92260 176 219 221 SPILLED 223 225 221 223 GROUP: MALE 9 - 1 PPM C92261 144 152 160 164 159 159 159 164 C92262 175 188 204 200 194 199 204 207 C92263 180 177 181 180 183 190 194 198 C92264 162 174 183 NOT TAKEN C92265 185 192 204 202 205 215 176 SPILLED C92266 141 149 163 158 168 165 162 162 C92267 157 149 157 152 149 150 151 148 C92268 185 204 209 230 235 236 250 244 C92269 177 197 211 206 214 212 230 SPILLED C92270 SPILLED 217 229 SPILLED SPILLED SPILLED 209 232 C92271 176 183 195 197 204 SPILLED 209 211 C92272 149 149 156 156 159 159 164 158 C92273 176 183 198 200 206 214 214 199 C92274 154 155 169 167 170 171 173 166 C92275 146 145 153 153 157 153 162 160 C92276 143 155 SPILLED 180 180 187 182 185 C92277 171 190 198 205 214 212 212 209 C92278 168 177 179 167 170 172 182 179 C92279 173 SPILLED 213 191 197 205 202 194 C92280 162 175 188 NOT TAKEN Covance 6329-228 3M T-6316.1 PAGE: 3 WEEK WEEK 910 197 184 221 214 195 188 197 208 183 178 182 200 225 220 162 166 211 203 196 199 221 SPILLED 166 175 158 155 244 228 254 SPILLED 192 218 208 218 164 167 233 211 176 189 163 170 175 177 210 204 184 182 191 194 4054 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: MALE 9 - 1 PPM C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 C92292 C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 150 159 162 188 179 158 162 182 166 159 161 139 173 159 175 145 140 175 154 163 191 179 162 181 192 180 164 140 180 174 186 160 165 164 176 193 161 164 184 171 167 159 139 185 156 214 151 147 179 153 176 188 194 167 187 200 192 160 148 184 185 185 167 176 174 184 166 177 178 176 173 181 183 186 177 NOT TAKEN 200 201 199 210 175 182 187 184 172 176 179 183 186 191 187 191 186 195 200 203 173 169 174 171 160 162 158 158 147 150 156 160 83 187 SPILLED 197 154 159 164 170 209 202 200 197 157 155 157 157 155 157 155 154 190 197 208 210 162 171 170 179 181 NOT TAKEN 199 192 200 198 204 205 213 213 182 182 190 190 196 196 195 198 197 200 202 214 193 183 198 184 173 177 188 189 155 156 155 159 187 180 177 183 195 204 204 207 192 190 194 200 176 179 182 178 194 181 208 213 187 183 180 178 192 189 205 208 184 183 154 153 163 159 198 192 178 191 197 204 161 160 153 161 207 200 183 SPILLED 180 188 215 224 184 178 200 197 208 192 185 193 196 195 165 163 185 208 209 223 194 201 Covance 6329-228 3M T-6316.1 PAGE: 4 WEEK WEEK 910 182 179 200 224 184 173 199 202 193 151 162 193 208 198 165 165 202 SPILLED 193 221 183 207 204 190 194 168 191 201 194 185 184 195 226 186 178 198 199 188 147 168 200 200 199 173 172 199 183 192 217 179 207 194 195 191 171 186 205 206 4055 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 9 - 1 PPM C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 C92323 C92324 C92325 C92326 C92327 C92328 C92329 C92330 154 172 167 175 157 167 157 177 172 155 162 188 168 185 161 179 164 146 174 156 171 171 190 197 174 168 194 185 164 160 190 174 194 177 192 175 164 180 GROUP: FEMALE 8 - 0 PPM C92331 C92332 C92333 C92334 C92335 C92336 C92337 C92338 C92339 SPILLED 129 156 SPILLED 122 109 159 134 126 SPILLED 117 SPILLED SPILLED 126 108 SPILLED SPILLED SPILLED 165 173 181 190 SPILLED 176 180 SPILLED 189 161 156 204 180 206 179 195 185 176 191 SPILLED 119 SPILLED 135 127 112 130 179 173 163 167 176 187 190 185 196 194 192 168 173 208 186 209 181 200 197 NOT TAKEN 204 166 175 187 208 SPILLED 190 SPILLED 198 197 170 165 203 199 207 180 204 SPILLED 195 SPILLED NOT TAKEN 161 161 124 121 141 SPILLED 158 SPILLED SPILLED 149 139 124 SPILLED SPILLED 173 167 182 176 207 178 189 186 185 198 163 162 203 185 214 180 195 SPILLED 199 SPILLED SPILLED 154 142 124 144 SPILLED 159 178 194 184 217 165 190 193 196 204 174 163 203 201 228 178 190 SPILLED 201 SPILLED 134 154 141 130 140 235 161 167 183 180 204 165 191 212 176 196 163 161 208 192 214 180 182 SPILLED 196 172 195 181 207 162 192 188 183 200 173 163 206 190 215 186 178 215 199 SPILLED 149 166 NOT TAKEN 126 152 190 156 157 SPILLED 164 122 154 219 160 173 194 181 210 159 194 188 183 201 168 161 206 194 219 179 183 205 207 138 144 SPILLED 122 142 SPILLED 150 4056 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92340 C92341 C92342 C92343 C92344 C92345 C92346 C92347 C92348 C92349 C92350 C92351 C92352 C92353 C92354 C92355 C92356 C92357 C92358 C92359 C92360 C92361 C92362 C92363 C92364 C92365 C92366 C92367 C92368 C92369 C92370 FEMALE 8 - 0 PPM 122 SPILLED SPILLED 139 123 112 SPILLED 108 SPILLED 131 132 111 156 SPILLED 118 SPILLED SPILLED 127 128 112 132 110 SPILLED 125 108 139 121 116 153 128 113 145 140 132 133 SPILLED 131 128 108 127 135 129 109 SPILLED 140 116 SPILLED SPILLED 142 136 108 137 SPILLED 108 131 112 SPILLED 131 114 169 130 117 137 142 123 137 SPILLED 149 SPILLED 112 SPILLED 206 140 108 SPILLED 167 123 127 165 150 126 115 143 121 121 143 107 171 145 112 166 134 129 145 SPILLED SPILLED 145 SPILLED 162 128 118 SPILLED 140 141 115 NOT TAKEN 141 138 NOT TAKEN 139 161 125 129 135 SPILLED 142 145 113 141 NOT TAKEN 121 SPILLED 141 127 132 150 121 137 SPILLED 155 SPILLED 121 SPILLED 149 138 110 138 136 136 SPILLED 133 125 139 119 122 137 113 SPILLED 116 SPILLED 145 118 136 171 128 139 200 153 137 113 156 142 143 105 SPILLED 131 145 186 128 127 148 127 SPILLED 141 113 190 124 151 149 119 140 144 124 141 SPILLED 160 141 113 149 152 150 110 148 142 132 156 137 131 137 132 130 147 117 159 122 164 158 135 148 148 118 142 SPILLED 161 132 123 125 159 137 117 SPILLED SPILLED 118 148 123 139 141 124 SPILLED SPILLED 109 140 127 165 152 128 Covance 6329-228 3M T-6316.1 PAGE: 6 WEEK 9 WEEK 10 132 138 127 127 SPILLED 146 127 125 121 144 126 112 SPILLED 133 SPILLED 143 127 148 139 123 115 133 113 SPILLED 121 149 152 128 132 145 121 SPILLED SPILLED 151 136 126 147 162 139 113 SPILLED 150 142 150 128 180 136 131 SPILLED 137 111 SPILLED 133 154 159 121 4057 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92371 C92372 C92373 C92374 C92375 C92376 C92377 C92378 C92379 C92380 C92381 C92382 C92383 C92384 C92385 C92386 C92387 C92388 C92389 C92390 C92391 C92392 C92393 C92394 C92395 C92396 C92397 C92398 C92399 C92400 FEMALE 8 - 0 PPM 147 SPILLED SPILLED 121 135 SPILLED 127 129 SPILLED 124 SPILLED 129 130 SPILLED SPILLED 158 121 SPILLED 127 SPILLED 131 SPILLED 139 SPILLED SPILLED SPILLED 118 SPILLED 119 121 134 SPILLED 135 118 143 127 132 129 115 128 143 116 131 165 129 141 120 SPILLED 151 SPILLED 133 157 SPILLED SPILLED SPILLED 124 122 SPILLED 115 126 143 153 142 124 150 114 133 143 120 127 155 112 131 124 132 144 SPILLED 126 125 166 141 152 155 139 125 150 129 138 115 135 162 SPILLED NOT TAKEN 134 147 119 129 139 122 145 SPILLED 119 155 129 156 SPILLED 137 SPILLED 129 SPILLED 148 126 134 141 141 SPILLED SPILLED 157 124 133 155 162 133 148 135 143 141 120 136 155 121 141 128 144 142 149 173 130 137 156 111 137 136 130 145 136 134 107 130 137 SPILLED 133 150 114 144 142 122 146 162 119 139 137 SPILLED 152 SPILLED 152 132 SPILLED 149 135 128 132 154 148 SPILLED 168 120 135 135 159 146 162 112 137 148 123 146 SPILLED 124 143 135 149 123 136 151 131 145 146 125 146 139 130 SPILLED 139 130 123 144 130 151 131 153 105 146 137 119 137 SPILLED 138 143 129 148 122 154 SPILLED 135 193 139 126 138 SPILLED 142 SPILLED 131 138 117 137 Covance 6329-228 3M T-6316.1 PAGE: 7 WEEK 9 WEEK 10 124 135 135 150 103 127 136 118 146 169 126 145 131 148 126 148 173 133 SPILLED 130 119 156 152 133 SPILLED 127 128 129 131 122 152 138 155 116 143 141 109 136 SPILLED 125 144 SPILLED SPILLED 131 160 SPILLED 132 160 SPILLED 117 126 156 139 SPILLED 130 132 113 144 4058 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 FEMALE 9 - 1 PPM 132 123 120 131 148 104 123 112 158 120 129 120 SPILLED 117 136 151 SPILLED 122 SPILLED SPILLED 123 107 SPILLED SPILLED 139 112 106 121 SPILLED 132 123 125 132 128 135 123 139 141 117 SPILLED 121 SPILLED SPILLED SPILLED 111 139 SPILLED SPILLED 124 SPILLED SPILLED 125 120 SPILLED 160 134 127 131 123 SPILLED 131 125 138 136 145 136 125 120 139 120 131 120 201 174 139 116 154 144 141 131 136 SPILLED 124 114 208 171 141 127 108 138 124 164 129 141 NOT TAKEN 137 155 128 114 154 128 129 119 SPILLED SPILLED 144 141 161 132 166 138 157 SPILLED 124 116 SPILLED SPILLED 140 121 122 136 131 138 131 150 148 148 127 SPILLED 147 127 139 126 SPILLED SPILLED 142 137 160 133 158 131 SPILLED SPILLED 128 122 SPILLED SPILLED 140 115 SPILLED 135 131 153 134 147 150 144 162 150 149 122 156 117 SPILLED 143 139 132 153 125 133 125 SPILLED SPILLED 149 125 145 SPILLED 132 137 SPILLED 137 143 136 140 151 144 150 149 133 143 123 143 125 139 169 146 139 145 122 137 117 SPILLED 160 127 121 159 SPILLED 129 121 148 139 125 148 139 144 132 140 SPILLED 130 138 128 SPILLED 130 SPILLED SPILLED 143 143 160 117 143 117 SPILLED SPILLED SPILLED 119 SPILLED 165 140 125 211 SPILLED SPILLED 135 144 Covance 6329-228 3M T-6316.1 PAGE: 8 WEEK 9 WEEK 10 149 132 149 117 134 131 123 130 119 SPILLED SPILLED 160 143 157 117 137 120 159 163 SPILLED 121 SPILLED 156 129 SPILLED 125 133 124 131 151 144 148 146 144 133 136 113 135 127 SPILLED 120 SPILLED 148 149 124 135 116 SPILLED SPILLED SPILLED 122 SPILLED SPILLED 131 113 126 131 126 132 147 4059 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 FEMALE 9 - 1 PPM 108 SPILLED 125 144 108 113 124 114 SPILLED 112 139 138 SPILLED 124 132 125 151 SPILLED 138 112 132 124 148 120 SPILLED 128 122 114 124 140 116 107 SPILLED 109 153 100 105 120 118 163 131 140 135 128 144 129 137 151 121 130 117 SPILLED 116 150 117 SPILLED 122 129 118 SPILLED 148 SPILLED 111 178 127 147 114 112 128 119 159 126 153 144 141 150 152 178 143 126 141 114 SPILLED 134 151 125 SPILLED 133 134 117 SPILLED 129 124 115 205 113 NOT TAKEN 116 111 131 127 153 130 162 147 SPILLED 156 150 155 160 135 141 132 SPILLED 131 158 130 198 129 135 120 123 138 126 117 SPILLED 120 122 122 133 127 133 135 158 152 138 163 147 152 154 127 157 134 SPILLED 138 148 133 SPILLED 141 135 122 138 SPILLED 122 110 SPILLED 123 118 125 130 124 131 129 159 163 SPILLED 162 SPILLED 151 144 SPILLED 139 131 SPILLED SPILLED 164 126 SPILLED 132 145 120 139 144 SPILLED 109 SPILLED 120 121 118 125 127 129 126 175 159 150 161 157 142 144 165 142 113 SPILLED SPILLED SPILLED SPILLED SPILLED 118 124 120 129 130 137 120 SPILLED 128 112 120 127 138 130 141 163 160 SPILLED 155 140 131 143 SPILLED 138 110 SPILLED 138 142 129 SPILLED SPILLED 134 124 147 SPILLED 127 Covance 6329-228 3M T-6316.1 PAGE: 9 WEEK 9 WEEK 10 109 129 119 115 116 122 121 136 115 156 135 SPILLED 150 132 145 142 139 131 122 163 137 141 123 133 140 131 122 197 SPILLED 131 112 SPILLED 125 112 125 116 122 127 120 161 155 SPILLED 165 155 SPILLED 143 SPILLED 140 130 156 145 145 137 SPILLED 128 145 121 SPILLED 146 122 4060 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 GROUP: FEMALE 9 - 1 PPM C92463 143 150 156 NOT TAKEN C92464 114 115 117 128 122 127 134 117 C92465 127 135 150 152 150 160 143 149 C92466 132 130 135 137 138 140 132 145 C92467 127 119 149 NOT TAKEN C92468 133 122 129 119 126 125 132 132 C92469 SPILLED 130 143 131 129 150 155 125 C92470 134 143 138 NOT TAKEN Covance 6329-228 3M T-6316.1 PAGE: 10 WEEK 9 WEEK 10 121 128 144 SPILLED 143 135 131 240 122 121 4061 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92191 C92193 C92194 C92195 C92197 C92198 C92199 C92200 C92201 C92202 C92203 C92204 C92205 C92206 C92208 C92209 C92210 C92211 C92212 C92213 C92214 C92215 C92217 C92218 C92219 C92220 C92221 C92222 C92223 C92224 C92225 C92226 MALE 8 - 0 166 179 195 172 176 NOT TAKEN 167 196 175 166 201 154 206 186 173 109 194 166 150 186 201 189 190 178 221 200 153 202 195 196 181 172 PPM 171 182 210 168 165 167 195 179 165 207 159 205 187 179 183 201 182 153 195 197 193 195 190 223 196 159 205 195 200 187 174 173 NOT TAKEN 164 165 165 170 190 NOT TAKEN 186 197 183 188 195 NOT TAKEN 190 195 191 204 173 NOT TAKEN 170 174 164 174 179 NOT TAKEN 176 180 175 183 181 NOT TAKEN 184 180 170 174 190 NOT TAKEN 176 189 186 174 176 NOT TAKEN 181 188 179 174 165 NOT TAKEN 164 166 164 174 208 NOT TAKEN 204 212 212 222 163 SPILLED 161 161 157 156 204 208 202 209 204 192 188 186 183 191 183 189 175 168 170 178 178 185 213 204 200 202 188 197 220 210 214 SPILLED 202 199 179 SPILLED 163 159 162 166 153 151 146 148 149 148 195 216 210 SPILLED SPILLED SPILLED 203 205 199 201 203 205 199 203 201 206 191 186 194 200 201 201 189 199 184 187 178 184 174 162 227 228 227 231 227 218 202 198 197 201 188 207 164 161 158 152 154 153 211 203 204 205 198 217 204 202 195 197 194 210 207 199 195 197 196 203 191 188 183 181 187 195 168 169 168 170 168 179 Covance 6329-228 3M T-6316.1 PAGE: 11 WEEK 28 174 SPILLED 189 173 186 170 167 190 173 217 155 205 SPILLED 194 219 204 165 156 SPILLED 207 199 198 184 220 204 164 218 212 201 194 184 WEEK 32 171 189 204 178 173 164 194 180 171 223 153 209 201 163 201 204 166 156 223 198 180 197 185 227 211 149 212 195 180 193 181 4062 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 8 - 0 PPM C92227 188 183 192 184 186 195 191 C92228 184 189 183 182 182 185 176 C92229 200 197 200 194 195 202 191 C92230 219 229 234 229 225 SPILLED 226 C92231 175 177 175 168 172 173 158 C92232 SPILLED SPILLED 234 221 230 247 244 C92233 188 198 196 186 186 186 189 C92234 216 219 221 220 211 219 208 C92235 168 169 165 162 163 166 154 C92236 162 164 162 163 148 153 149 C92237 201 202 206 203 196 202 197 C92238 185 190 188 188 192 187 192 C92239 191 192 188 197 197 204 198 C92240 206 210 213 203 214 210 203 C92241 167 180 173 179 169 170 158 C92242 187 190 195 197 188 190 188 C92243 200 206 210 213 208 207 210 C92244 206 205 206 210 208 202 196 C92245 216 214 217 219 222 226 215 C92246 190 204 210 200 193 187 191 C92247 211 221 217 209 215 214 202 C92248 196 213 198 207 199 203 194 C92249 178 182 178 181 190 191 173 C92250 200 201 207 208 203 213 196 C92251 173 178 179 174 173 179 173 C92252 SPILLED 240 217 225 193 SPILLED 180 C92253 195 191 200 195 193 196 185 C92254 212 209 214 211 214 211 203 C92255 180 181 186 NOT TAKEN C92256 200 208 212 NOT TAKEN C92257 174 161 155 NOT TAKEN 196 187 209 229 148 248 202 222 162 154 205 193 206 205 173 190 206 203 220 180 209 198 175 202 176 180 200 208 Covance 6329-228 3M T-6316.1 PAGE: 12 WEEK 28 WEEK 32 206 184 211 SPILLED 166 247 199 213 166 149 206 203 218 205 178 201 205 204 221 178 205 203 187 204 177 183 202 206 210 192 221 210 165 239 197 200 156 152 207 196 195 209 184 188 215 210 225 175 221 202 195 206 186 176 163 202 4063 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 8 - 0 PPM C92258 205 211 218 NOT TAKEN C92260 219 239 222 NOT TAKEN GROUP: MALE 9 - 1 PPM C92261 158 167 166 NOT TAKEN 167 171 177 174 C92262 200 207 207 NOT TAKEN 206 221 203 196 C92263 193 198 200 NOT TAKEN 210 213 203 205 C92265 SPILLED 234 232 NOT TAKEN 236 226 210 200 C92266 175 180 180 NOT TAKEN 179 177 146 175 C92267 160 163 166 NOT TAKEN 161 158 158 164 C92268 225 229 238 NOT TAKEN SPILLED 243 236 237 C92269 SPILLED SPILLED SPILLED NOT TAKEN SPILLED 202 236 167 C92270 174 214 184 NOT TAKEN 201 185 173 176 C92271 202 221 227 NOT TAKEN 213 214 221 221 C92272 165 168 179 175 171 175 172 186 C92273 203 204 215 197 207 218 SPILLED 228 C92274 185 187 184 168 171 174 171 173 C92275 166 171 166 162 165 159 162 162 C92276 177 183 186 188 183 184 180 181 C92277 207 209 215 208 204 201 195 185 C92278 173 180 181 173 179 177 165 170 C92279 189 191 199 192 194 192 178 195 C92281 174 171 183 174 183 173 169 167 C92282 185 182 185 182 180 179 186 197 C92283 SPILLED 196 202 188 194 189 173 182 C92285 225 217 225 225 228 226 214 212 C92286 184 193 193 182 182 197 186 186 C92287 175 183 188 181 186 186 184 187 C92288 196 205 201 197 199 208 207 205 C92289 196 193 197 191 194 192 186 183 Covance 6329-228 3M T-6316.1 PAGE: 13 WEEK 28 WEEK 32 182 213 202 226 185 166 246 SPILLED 193 228 SPILLED SPILLED 173 167 193 206 172 191 178 199 189 224 191 182 214 189 190 200 204 227 182 156 249 249 185 221 176 234 180 171 188 209 175 188 183 208 176 233 181 198 204 196 4064 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 9 - 1 PPM C92290 189 189 202 197 201 195 174 182 C92291 150 155 158 163 163 153 152 162 C92292 174 178 182 179 176 176 181 192 C92293 196 219 210 207 199 204 198 207 C92294 195 192 196 184 202 195 171 180 C92295 201 199 190 187 206 197 175 193 C92296 170 178 180 179 179 178 183 188 C92297 167 175 171 180 174 166 175 183 C92298 202 208 221 211 215 210 188 217 C92299 187 195 196 178 185 184 SPILLED 182 C92301 192 202 203 203 209 203 197 215 C92302 220 227 228 213 213 218 212 226 C92303 176 182 186 183 183 189 185 194 C92304 193 208 205 205 202 198 208 206 C92305 190 202 211 212 214 213 184 186 C92306 196 207 210 205 210 211 195 205 C92307 194 203 200 199 199 200 188 204 C92308 163 169 176 157 157 154 SPILLED 166 C92309 184 189 180 173 177 173 172 174 C92310 198 207 215 207 220 222 201 192 C92311 193 201 202 193 201 205 203 210 C92312 165 170 174 171 172 173 177 176 C92313 191 197 204 201 210 207 202 196 C92314 182 189 191 179 188 192 183 184 C92315 213 211 205 210 216 219 206 201 C92316 160 171 160 171 165 167 149 154 C92317 196 190 192 196 204 198 191 192 C92318 193 202 204 198 194 211 197 199 C92319 188 200 196 194 190 169 202 195 C92320 207 206 196 206 201 203 196 194 C92321 169 174 170 166 175 173 163 174 Covance 6329-228 3M T-6316.1 PAGE: 14 WEEK 28 WEEK 32 179 166 189 212 180 191 191 187 204 SPILLED 204 205 199 214 196 191 211 164 181 193 196 176 208 189 192 158 201 194 218 196 173 185 163 198 213 189 195 197 194 207 182 210 241 197 222 192 203 211 184 184 190 214 167 209 195 199 154 199 193 196 198 171 4065 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: MALE 9 - 1 PPM C92322 171 171 168 163 163 163 165 170 C92323 209 215 221 209 211 214 207 209 C92324 188 203 195 201 202 209 200 201 C92325 215 223 223 NOT TAKEN C92326 180 201 191 NOT TAKEN C92327 182 184 178 NOT TAKEN C92328 SPILLED 200 195 NOT TAKEN C92330 201 203 205 NOT TAKEN GROUP: FEMALE 8 - 0 PPM C92331 151 SPILLED 132 NOT TAKEN 167 SPILLED 159 155 C92333 136 141 144 NOT TAKEN 122 128 141 SPILLED C92334 141 146 146 NOT TAKEN 151 134 142 140 C92336 127 114 123 NOT TAKEN 119 129 153 149 C92337 142 147 142 NOT TAKEN 146 148 145 165 C92338 180 SPILLED 194 NOT TAKEN SPILLED SPILLED 157 128 C92339 160 171 160 NOT TAKEN 153 170 151 156 C92340 143 130 138 NOT TAKEN 133 141 125 130 C92341 133 SPILLED 155 NOT TAKEN 140 155 150 137 C92342 121 123 133 NOT TAKEN 134 137 139 127 C92343 140 160 154 149 157 157 140 147 C92344 SPILLED SPILLED 136 125 135 131 129 159 C92345 148 134 143 150 146 139 177 186 C92346 126 115 124 132 122 123 128 123 C92347 120 SPILLED 132 140 120 123 116 130 C92348 SPILLED 124 133 129 123 SPILLED 114 114 C92349 150 133 157 132 146 151 134 145 C92350 131 131 121 126 126 126 125 130 C92351 108 111 109 105 97 110 110 109 C92353 SPILLED 125 194 164 128 SPILLED 133 134 Covance 6329-228 3M T-6316.1 PAGE: 15 WEEK 28 WEEK 32 177 170 222 210 208 206 165 SPILLED 156 159 177 170 173 165 162 152 175 131 95 137 134 121 148 133 119 141 195 212 155 136 171 146 177 155 177 138 171 122 126 133 119 157 132 117 145 4066 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92354 C92356 C92357 C92358 C92359 C92360 C92361 C92362 C92363 C92364 C92365 C92367 C92368 C92369 C92370 C92371 C92372 C92374 C92375 C92376 C92377 C92378 C92379 C92380 C92381 C92382 C92383 C92384 C92385 C92386 C92387 FEMALE 8 - 0 PPM 145 SPILLED 142 124 SPILLED 133 125 SPILLED SPILLED 106 SPILLED 114 150 151 140 109 125 135 153 111 118 136 113 140 150 127 140 125 135 119 131 140 116 140 118 195 130 128 SPILLED 124 104 161 125 125 139 130 113 136 126 150 103 117 128 107 138 SPILLED 121 146 120 145 122 136 136 SPILLED 149 127 159 114 136 118 141 109 SPILLED 123 148 142 124 114 136 127 151 107 139 136 110 136 150 122 135 121 142 119 145 137 SPILLED 151 117 SPILLED 125 128 121 145 103 148 131 135 144 125 119 126 125 SPILLED 107 148 129 113 141 SPILLED 116 134 125 SPILLED 126 137 142 130 145 122 144 130 125 118 135 110 148 126 126 143 141 112 135 108 156 108 143 131 107 138 SPILLED 125 142 122 141 118 141 140 SPILLED 142 119 SPILLED 129 SPILLED SPILLED SPILLED 107 SPILLED 131 119 150 125 113 121 58 155 107 135 121 112 137 147 129 156 120 133 118 146 124 128 136 122 195 134 124 SPILLED 130 108 134 124 132 148 130 116 134 151 143 115 134 151 127 160 176 119 137 116 136 117 130 147 132 137 133 193 131 134 123 170 118 129 140 145 152 139 301 142 135 168 123 120 155 132 153 168 128 139 117 150 130 162 Covance 6329-228 3M T-6316.1 PAGE: 16 WEEK 28 WEEK 32 168 141 166 135 202 148 126 134 157 133 SPILLED 129 153 SPILLED 160 142 157 157 164 127 122 166 139 159 198 142 146 118 167 160 163 151 147 170 137 SPILLED 134 125 136 163 135 136 129 147 161 170 125 146 177 173 127 118 158 140 162 185 148 139 120 161 149 150 4067 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: FEMALE 8 - 0 PPM C92388 C92389 C92390 C92391 C92392 C92393 C92394 C92395 C92396 C92397 C92398 C92399 C92400 SPILLED 120 SPILLED SPILLED 121 130 135 128 SPILLED 118 133 107 141 SPILLED 126 SPILLED SPILLED 121 123 127 144 SPILLED 129 131 111 125 GROUP: FEMALE 9 - 1 PPM C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 145 129 134 SPILLED 118 127 116 118 113 SPILLED SPILLED 133 126 133 100 147 162 142 122 SPILLED 131 119 124 125 SPILLED SPILLED 132 139 145 114 132 132 SPILLED SPILLED 118 149 133 128 167 133 132 112 133 149 146 143 134 120 130 117 123 125 210 146 148 138 127 116 SPILLED 126 SPILLED 137 109 153 127 134 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 122 132 136 122 166 118 130 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 181 137 146 SPILLED 144 136 139 140 128 131 113 132 120 153 126 172 129 139 SPILLED 148 128 SPILLED SPILLED 114 125 126 132 151 139 148 121 132 130 119 120 117 SPILLED SPILLED 181 131 133 125 SPILLED 121 133 SPILLED 115 137 117 200 146 143 149 115 135 148 117 120 114 SPILLED SPILLED 170 141 148 127 154 134 SPILLED 196 113 137 129 142 153 152 155 121 148 144 119 136 125 189 122 183 140 134 124 Covance 6329-228 3M T-6316.1 PAGE: 17 WEEK 28 WEEK 32 143 SPILLED SPILLED SPILLED 119 198 144 145 144 129 137 160 117 203 146 134 163 167 167 155 145 160 146 140 148 137 166 162 130 119 139 129 118 131 184 173 133 161 189 164 152 145 151 154 128 131 4068 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92417 C92418 C92419 C92420 C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 C92432 C92433 C92434 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 FEMALE 9 - 1 PPM 129 116 SPILLED 139 SPILLED 110 SPILLED SPILLED 114 SPILLED SPILLED 125 129 124 123 98 106 115 118 127 112 114 116 118 133 143 SPILLED 139 134 149 133 137 125 151 SPILLED 134 114 148 167 120 120 145 131 132 140 126 112 115 115 116 126 119 125 123 122 135 135 SPILLED 147 SPILLED SPILLED 147 131 131 SPILLED 173 132 117 172 161 128 131 SPILLED 129 122 143 145 108 109 125 123 138 123 122 140 120 153 154 SPILLED 164 145 152 160 122 117 192 177 124 120 SPILLED SPILLED 127 SPILLED SPILLED 117 SPILLED 128 146 116 SPILLED 132 114 127 120 126 123 SPILLED 160 137 SPILLED 155 116 144 166 123 119 SPILLED 189 126 116 SPILLED 180 120 SPILLED SPILLED 122 120 126 130 106 108 125 118 118 115 128 138 113 145 154 SPILLED 155 138 162 157 129 114 SPILLED SPILLED 117 110 SPILLED SPILLED 120 SPILLED SPILLED 132 127 128 121 112 119 136 127 120 121 137 132 118 143 136 SPILLED 151 144 SPILLED 162 115 132 SPILLED SPILLED 119 115 157 238 121 226 SPILLED 130 129 130 127 121 158 129 115 136 125 145 119 146 146 150 SPILLED 153 142 186 156 129 127 SPILLED SPILLED 137 126 SPILLED SPILLED 137 SPILLED 122 136 131 132 147 129 115 122 121 147 132 130 132 148 177 144 167 168 137 188 158 Covance 6329-228 3M T-6316.1 PAGE: 18 WEEK 28 WEEK 32 144 127 SPILLED 234 145 141 172 213 125 154 121 135 142 135 155 133 124 138 150 179 137 126 143 150 159 159 SPILLED 169 157 208 167 136 132 158 221 149 137 165 218 141 143 143 110 174 132 153 128 126 132 121 168 141 119 132 155 163 165 179 176 168 203 170 4069 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 GROUP: C92449 C92450 C92451 C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 C92464 C92465 C92466 C92468 C92469 FEMALE 9 - 1 PPM SPILLED 127 126 152 154 141 153 129 125 132 104 SPILLED 144 150 123 139 SPILLED SPILLED 119 SPILLED 144 140 161 149 161 125 SPILLED 134 138 121 SPILLED 147 133 134 151 144 132 114 131 163 134 166 148 150 129 SPILLED 139 139 128 192 157 155 133 141 158 123 121 161 142 139 169 151 156 140 124 134 117 124 SPILLED SPILLED 132 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 159 141 131 171 146 147 130 125 130 128 137 239 158 139 142 154 148 168 142 143 134 136 132 142 130 SPILLED SPILLED 141 130 150 166 SPILLED 128 120 120 153 142 146 134 SPILLED 149 142 134 154 155 151 121 121 116 112 128 149 133 207 163 135 Covance 6329-228 3M T-6316.1 PAGE: 19 WEEK 28 WEEK 32 142 164 160 158 144 145 130 122 133 160 122 SPILLED 175 146 138 161 159 SPILLED 139 140 132 118 156 159 124 180 172 125 4070 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 C92204 C92205 C92206 C92208 C92209 C92210 C92211 C92212 C92213 C92214 C92215 C92217 C92218 C92219 C92220 C92221 C92222 C92223 C92224 C92225 C92226 C92227 MALE 8 - 0 PPM 172 168 SPILLED 198 187 184 174 168 175 167 168 161 180 186 177 172 174 172 231 217 150 156 190 192 188 184 194 192 197 188 200 193 168 158 157 157 203 206 192 168 193 198 195 191 173 168 219 221 200 200 157 157 208 209 195 182 200 198 191 191 186 175 208 203 171 202 194 165 162 163 169 164 167 SPILLED 146 196 185 199 198 199 206 155 111 188 206 181 161 215 188 157 198 193 193 185 184 199 172 199 182 160 165 156 182 165 160 227 148 195 187 201 193 214 165 153 129 188 196 179 162 215 182 155 197 197 178 183 191 182 170 165 172 171 245 SPILLED SPILLED 197 185 185 202 193 166 168 160 146 160 157 174 183 204 198 174 175 183 185 166 164 176 169 238 230 240 244 153 158 159 174 201 181 180 197 190 199 193 202 212 197 187 194 197 214 215 SPILLED 231 168 157 153 165 174 198 193 199 193 173 134 191 178 192 170 165 168 222 214 223 185 SPILLED 175 151 152 150 199 190 198 187 194 185 194 180 176 188 191 168 185 190 193 205 197 145 186 174 228 180 156 193 196 186 198 209 Covance 6329-228 3M T-6316.1 PAGE: 20 WEEK 68 WEEK 72 172 185 201 158 196 187 163 SPILLED 181 178 204 189 235 166 173 117 206 191 224 169 160 204 187 52 203 216 172 209 205 146 191 184 SPILLED 266 178 89 212 191 212 175 167 151 204 194 238 128 158 207 202 214 229 4071 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 21 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 8 - 0 PPM C92228 181 177 186 178 171 149 80 NOT TAKEN C92229 214 201 194 213 214 208 198 214 227 228 C92230 201 201 202 195 194 C92231 172 170 159 168 177 176 186 193 185 192 C92232 227 255 240 245 237 231 240 242 231 SPILLED C92233 194 188 190 186 184 184 188 195 194 215 C92234 220 217 229 162 235 211 237 239 244 243 C92235 159 162 164 139 162 148 141 159 157 157 C92236 148 151 154 146 149 148 159 171 157 171 C92237 202 193 207 206 196 225 234 241 253 244 C92238 190 194 200 188 194 C92239 187 197 189 173 189 183 184 190 202 218 C92240 203 209 198 193 210 188 195 214 208 213 C92241 187 192 174 178 183 189 200 192 190 206 C92242 180 202 191 189 193 187 192 144 C92243 210 209 202 196 201 180 219 189 95 C92244 210 204 203 210 210 185 190 209 C92245 212 208 197 159 207 SPILLED 217 22 NOT TAKEN C92246 182 180 172 177 186 C92247 212 204 206 205 213 C92248 194 202 193 189 196 192 170 119 46 C92249 183 179 183 182 187 149 201 159 169 NOT TAKEN C92250 197 207 189 196 198 203 212 216 217 209 C92251 178 190 180 173 181 175 189 188 197 206 C92252 167 160 159 178 163 155 162 167 176 180 C92253 198 194 158 168 192 C92254 184 207 204 208 203 207 212 218 226 221 4072 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 9 - 1 PPM C92261 192 185 183 179 170 181 187 190 C92262 199 195 188 196 187 190 186 194 C92263 200 195 197 199 194 188 208 209 C92265 214 221 237 217 200 199 199 201 C92266 161 169 170 172 172 174 179 187 C92267 160 160 160 159 154 C92268 238 241 237 246 237 237 243 261 C92269 243 241 235 228 238 232 244 248 C92270 183 176 181 182 176 176 179 179 C92271 229 223 213 217 213 216 225 235 C92272 SPILLED 170 168 166 165 166 186 183 C92273 210 210 C92274 173 176 149 170 170 C92275 175 164 169 173 162 160 170 172 C92276 184 188 182 174 181 173 173 182 C92277 206 201 199 200 196 162 NOT TAKEN C92278 175 171 164 177 165 175 177 181 C92279 189 189 176 174 180 184 197 198 C92281 176 173 167 177 169 160 176 189 C92282 203 191 199 190 177 182 186 197 C92283 180 179 175 174 181 183 177 183 C92285 232 225 230 234 225 229 230 220 C92286 182 179 172 176 171 C92287 184 186 182 191 174 C92288 205 208 204 212 211 C92289 184 183 177 178 179 173 207 195 C92290 178 173 164 160 148 72 60 C92291 166 163 155 162 160 143 167 174 C92292 197 196 189 181 184 128 93 77 C92293 210 202 C92294 180 172 189 171 163 174 181 171 Covance 6329-228 3M T-6316.1 PAGE: 22 WEEK 68 WEEK 72 187 184 193 188 202 210 201 189 190 190 253 261 259 245 194 193 219 218 193 177 174 169 171 35 171 193 196 210 193 193 158 159 189 187 233 85 192 182 177 177 125 134 4073 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: MALE 9 - 1 PPM C92295 191 177 186 196 201 194 202 176 C92296 188 186 185 183 167 165 166 172 C92297 SPILLED 174 179 174 174 161 162 179 C92298 189 192 210 201 195 189 196 76 C92299 180 181 173 164 SPILLED SPILLED SPILLED 180 C92301 205 190 191 194 195 200 206 209 C92302 224 218 232 220 219 210 234 164 C92303 SPILLED 186 190 184 182 C92304 209 211 200 207 202 199 204 221 C92305 194 188 185 186 190 193 161 C92306 192 193 187 200 194 188 195 197 C92307 189 202 197 198 185 211 201 217 C92308 183 190 158 173 163 208 SPILLED SPILLED C92309 176 172 172 179 166 165 170 182 C92310 197 196 190 187 190 170 194 210 C92311 SPILLED 208 198 204 203 206 229 226 C92312 162 153 150 146 142 147 143 137 C92313 208 207 201 205 197 195 197 217 C92314 184 187 181 175 178 169 172 189 C92315 192 192 188 181 195 C92316 155 149 150 147 154 149 151 157 C92317 202 212 205 199 191 C92318 182 177 181 177 173 182 181 189 C92319 197 187 181 192 173 176 185 187 C92320 200 192 199 193 199 C92321 166 165 158 149 166 150 158 167 C92322 168 162 166 161 157 156 167 178 C92323 196 196 194 197 185 194 200 216 C92324 210 196 203 226 206 Covance 6329-228 3M T-6316.1 PAGE: 23 WEEK 68 WEEK 72 184 162 183 190 203 238 210 202 224 215 186 213 220 215 186 154 195 SPILLED 167 172 224 202 158 187 193 213 237 223 230 227 SPILLED 190 123 228 230 180 168 197 192 176 180 221 4074 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 8 - 0 PPM C92331 168 157 159 160 204 171 SPILLED SPILLED C92333 196 169 164 137 178 SPILLED SPILLED 174 C92334 154 131 129 135 146 158 146 152 C92336 125 155 127 149 136 157 160 164 C92337 160 173 167 157 163 158 181 179 C92338 139 SPILLED 137 137 141 136 152 74 C92339 173 173 169 164 165 212 186 186 C92340 156 155 167 161 SPILLED 201 188 170 C92341 170 157 170 161 156 C92342 125 135 124 131 C92343 152 140 147 151 133 138 C92344 133 126 125 130 135 127 142 153 C92346 117 145 145 148 102 63 SPILLED C92347 117 140 95 NOT TAKEN C92348 119 113 125 126 122 136 125 147 C92349 165 152 148 137 155 140 147 166 C92350 127 132 126 127 133 137 134 134 C92351 110 117 121 107 100 132 111 104 C92353 149 136 137 141 141 136 136 164 C92354 143 140 145 146 125 142 153 141 C92356 144 130 145 155 165 C92357 177 173 173 171 180 C92358 135 132 135 136 139 140 132 127 C92359 167 172 151 SPILLED 156 165 SPILLED 177 C92360 133 128 136 139 142 135 128 163 C92361 131 145 136 133 136 138 148 156 C92362 127 119 122 134 130 143 116 149 C92363 SPILLED 151 146 153 126 141 153 174 C92364 135 122 142 110 71 62 54 C92365 122 114 139 130 123 C92367 129 119 124 132 139 124 111 141 Covance 6329-228 3M T-6316.1 PAGE: 24 WEEK 68 WEEK 72 203 SPILLED 197 150 188 127 188 176 156 139 154 141 120 200 165 162 177 149 158 200 178 130 221 193 185 161 148 204 142 149 148 196 148 128 170 167 160 181 168 163 183 186 136 4075 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 8 - 0 PPM C92368 146 128 140 123 165 162 153 160 C92369 164 157 156 166 167 166 179 181 C92370 153 149 159 184 159 162 173 183 C92371 126 127 141 134 138 123 145 151 C92372 135 143 150 155 160 C92374 156 147 155 146 155 158 161 158 C92375 164 172 168 186 191 185 180 201 C92376 129 133 126 131 165 C92377 132 132 97 110 147 NOT TAKEN C92378 150 148 158 145 167 143 143 153 C92379 142 139 136 138 160 149 149 SPILLED C92380 150 153 133 197 139 151 193 168 C92381 168 167 161 172 171 157 153 82 C92382 151 148 134 145 150 2 C92383 139 133 131 118 145 144 151 158 C92384 123 126 139 130 140 142 154 153 C92385 153 157 160 158 147 167 164 170 C92386 149 145 151 141 142 137 144 153 C92387 142 141 145 151 163 150 153 171 C92388 125 150 143 133 157 C92389 124 121 140 118 125 C92390 125 130 133 126 123 C92391 177 162 169 159 176 163 175 162 C92392 125 118 115 109 39 142 181 NOT TAKEN C92393 181 191 167 186 179 183 192 202 C92394 141 142 151 143 163 132 158 163 C92395 143 130 140 135 145 Covance 6329-228 3M T-6316.1 PAGE: 25 WEEK 68 WEEK 72 164 171 156 169 191 213 162 155 146 142 207 206 128 4 82 167 75 6 163 168 171 152 181 192 144 146 170 SPILLED 223 234 188 208 165 169 4076 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: FEMALE 9 - 1 PPM C92401 154 152 168 162 173 157 183 172 C92403 167 139 151 92 C92404 158 164 143 152 147 149 163 158 C92405 139 126 136 131 146 143 133 150 C92406 150 148 124 136 147 C92407 145 154 171 138 172 C92408 135 120 135 122 137 125 125 126 C92409 151 138 148 140 151 141 157 156 C92410 135 127 121 115 130 133 132 137 C92411 168 163 159 215 175 C92412 119 131 147 134 SPILLED 142 SPILLED 147 C92413 166 140 152 156 168 120 176 204 C92414 143 139 134 144 132 149 152 C92415 153 133 151 156 144 159 172 167 C92416 134 123 130 121 129 108 124 121 C92417 148 121 146 140 147 132 151 149 C92418 125 124 132 131 131 C92419 129 133 124 117 116 122 133 206 C92420 188 192 175 193 SPILLED 183 178 191 C92421 157 144 138 146 120 139 171 152 C92422 132 135 123 138 SPILLED C92423 SPILLED 183 152 168 SPILLED 150 154 143 C92424 SPILLED 209 SPILLED 245 SPILLED C92425 149 130 130 130 133 135 148 130 C92426 133 128 137 130 141 122 121 123 C92427 SPILLED SPILLED 174 172 263 163 SPILLED C92428 146 128 129 134 115 128 80 C92429 135 139 131 126 120 79 114 C92430 139 131 144 141 127 138 139 141 C92431 166 133 159 141 127 163 144 175 C92432 136 131 134 133 149 138 142 151 Covance 6329-228 3M T-6316.1 PAGE: 26 WEEK 68 WEEK 72 195 190 181 166 141 166 143 138 148 179 137 141 159 150 162 212 160 133 147 137 168 177 123 153 162 168 174 167 168 165 144 143 127 121 159 156 185 149 146 77 4077 Covance 6329-212 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 GROUP: C92433 C92434 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 FEMALE 9 - 1 PPM 131 134 127 166 122 SPILLED 138 SPILLED 161 160 146 178 139 188 147 146 153 SPILLED 173 143 146 131 131 144 159 137 147 168 151 125 130 120 153 133 114 131 162 165 141 SPILLED 181 166 190 158 148 147 148 160 135 146 134 130 130 150 120 SPILLED 163 151 120 126 132 144 134 128 128 178 173 171 SPILLED 187 148 197 169 150 153 138 165 134 152 135 130 125 144 129 130 137 181 130 129 130 85 128 117 168 170 172 170 SPILLED 185 152 196 157 160 138 133 156 143 165 127 134 123 153 129 140 170 172 128 139 127 SPILLED 108 123 179 179 153 148 205 160 199 134 187 154 SPILLED 163 132 148 134 139 131 147 134 129 185 143 139 115 127 132 165 180 SPILLED 199 179 SPILLED 158 145 SPILLED SPILLED 141 161 124 140 SPILLED 163 139 128 176 159 149 127 111 220 170 192 SPILLED 214 162 211 154 149 195 SPILLED 163 160 134 128 131 138 127 137 68 136 156 SPILLED 125 130 80 185 151 215 169 198 61 158 206 SPILLED 141 122 146 155 124 2 134 137 167 Covance 6329-228 3M T-6316.1 PAGE: 27 WEEK 68 WEEK 72 148 154 132 148 136 141 NOT TAKEN 172 145 200 174 203 78 170 216 213 177 155 128 144 157 146 149 174 147 167 201 145 193 172 148 157 222 SPILLED 176 132 148 152 148 151 155 165 4078 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 8 - 0 PPM C92191 173 151 157 155 152 169 191 NOT TAKEN C92193 SPILLED SPILLED 203 192 174 182 191 176 C92194 183 192 187 188 182 187 70 C92197 139 132 141 140 141 134 173 126 C92200 37 C92201 194 195 192 185 211 C92202 155 141 126 C92203 252 212 216 220 173 136 SPILLED SPILLED C92204 176 98 C92206 NOT TAKEN C92208 208 196 188 200 188 220 227 251 C92209 191 191 162 177 167 143 141 180 C92210 218 187 210 217 207 98 C92212 168 158 152 164 153 145 132 NOT TAKEN C92214 167 136 5 C92215 NOT TAKEN C92217 220 C92218 180 180 172 168 140 149 59 117 C92219 240 225 172 C92220 85 C92221 158 146 139 132 92 C92222 179 195 189 171 200 219 212 216 C92224 197 167 181 172 204 211 SPILLED C92226 211 184 145 102 C92227 230 208 199 202 216 83 84 C92229 215 160 164 C92231 195 175 172 169 170 174 195 188 C92232 251 240 214 220 206 91 NOT TAKEN C92233 212 199 192 195 182 168 29 SPILLED C92234 250 93 8 C92235 147 131 152 118 123 156 C92236 178 151 149 54 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 28 4079 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 8 - 0 PPM C92237 C92239 C92240 C92241 C92250 C92251 C92252 C92254 236 204 214 186 230 208 175 207 220 184 174 185 227 179 167 249 GROUP: MALE 9 - 1 PPM C92261 C92262 C92263 C92265 C92266 C92268 C92270 C92271 C92272 C92275 C92278 C92279 C92281 C92282 C92283 C92285 C92289 C92291 C92294 C92295 183 179 202 130 194 245 197 226 174 170 176 184 183 160 207 NOT TAKEN 193 167 244 202 183 181 186 NOT TAKEN 179 203 191 SPILLED 158 163 170 129 174 156 186 177 175 204 181 219 194 190 183 NOT TAKEN 166 69 177 186 197 178 186 186 168 185 171 178 125 162 152 187 169 173 113 184 220 199 123 162 161 173 184 186 166 195 195 153 170 200 SPILLED 169 152 178 165 173 71 184 SPILLED 186 29 157 181 213 196 163 110 190 154 184 160 137 176 156 178 73 189 127 SPILLED 143 77 SPILLED 215 187 153 189 SPILLED 181 168 69 191 186 SPILLED 174 185 NOT TAKEN 190 211 176 176 204 144 172 157 146 158 143 NOT TAKEN 20 166 115 194 179 157 169 144 187 NOT TAKEN 202 182 152 154 135 193 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 29 4080 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 9 - 1 PPM C92297 C92299 C92301 C92302 C92304 C92306 C92307 C92308 C92309 C92311 C92313 C92314 C92316 C92318 C92319 C92321 C92322 C92323 183 169 190 SPILLED 206 194 252 218 203 206 201 183 210 182 207 SPILLED 191 183 200 213 221 211 192 184 170 147 201 185 159 177 172 177 176 153 186 201 GROUP: FEMALE 8 - 0 PPM C92331 C92333 C92334 C92336 C92337 C92339 C92340 C92344 C92348 C92349 SPILLED SPILLED SPILLED 158 160 205 131 163 171 150 141 SPILLED SPILLED 90 95 176 135 147 169 168 178 202 202 196 160 SPILLED 175 211 216 181 SPILLED 182 164 148 162 197 90 SPILLED 157 181 137 144 168 173 213 184 187 152 SPILLED 179 204 224 132 148 186 158 158 165 187 144 SPILLED 143 175 131 156 154 172 109 183 187 148 182 173 200 215 SPILLED 121 161 176 147 176 199 157 169 NOT TAKEN NOT TAKEN 185 143 154 170 219 170 80 203 179 150 162 199 NOT TAKEN SPILLED 146 176 129 116 151 184 134 170 174 145 190 141 161 166 249 114 199 197 159 181 196 190 156 88 128 162 95 101 130 233 183 169 150 194 147 162 142 169 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 30 4081 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 8 - 0 PPM C92350 C92351 C92353 C92354 C92358 C92359 C92360 C92361 C92362 C92363 C92367 C92368 C92369 C92370 C92371 C92374 C92375 C92380 C92383 C92384 C92385 C92386 C92391 C92393 C92394 139 132 172 SPILLED 176 SPILLED 155 162 SPILLED 166 SPILLED 163 172 196 151 158 171 109 166 136 161 161 209 206 82 136 115 140 SPILLED 155 148 110 144 158 116 130 161 143 152 133 157 89 SPILLED 150 162 135 SPILLED 157 178 126 GROUP: FEMALE 9 - 1 PPM C92401 C92404 C92405 SPILLED 166 161 127 60 152 104 114 145 SPILLED 151 158 131 114 156 125 174 158 174 123 151 32 157 126 24 132 119 185 SPILLED 76 135 78 100 129 118 141 54 181 130 145 126 82 SPILLED 174 142 132 SPILLED 151 154 127 123 177 146 164 165 180 174 118 138 212 151 NOT TAKEN 113 142 134 118 152 53 128 51 115 145 NOT TAKEN 132 124 142 176 135 130 105 165 151 147 131 128 112 153 SPILLED 132 147 82 202 NOT TAKEN 157 SPILLED 92 101 156 82 148 180 157 159 NOT TAKEN 136 158 135 163 136 61 NOT TAKEN 144 126 18 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 31 4082 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: C92408 C92409 C92410 C92412 C92413 C92415 C92416 C92417 C92419 C92421 C92423 C92425 C92426 C92430 C92431 C92432 C92434 C92436 C92439 C92440 C92443 C92444 C92445 C92446 C92447 C92450 C92451 C92452 C92453 C92454 C92455 FEMALE 9 - 1 PPM 144 162 144 128 185 139 144 188 133 159 158 179 135 143 166 143 173 139 126 142 207 SPILLED 199 156 63 128 226 184 183 110 153 130 SPILLED 128 140 165 156 136 165 141 144 144 153 134 133 159 156 120 128 NOT TAKEN 177 162 206 3 80 48 SPILLED SPILLED 155 73 151 128 165 135 114 177 164 128 49 124 151 149 161 136 165 159 SPILLED 164 120 192 SPILLED 199 82 89 203 155 148 149 146 162 139 139 166 111 129 25 137 166 165 159 142 149 163 157 157 117 178 133 187 83 72 197 177 128 157 131 155 SPILLED NOT TAKEN 130 18 142 139 140 125 139 145 140 147 117 132 119 123 93 136 156 85 130 126 153 139 143 134 123 97 159 163 192 199 144 132 NOT TAKEN 142 140 185 128 150 154 148 159 172 165 156 176 149 NOT TAKEN 117 117 132 134 179 167 134 111 130 162 SPILLED SPILLED 160 NOT TAKEN 52 SPILLED SPILLED SPILLED 166 198 SPILLED SPILLED 75 63 153 160 58 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 32 4083 Appendix 4 Individual Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 9 - 1 PPM C92456 SPILLED 131 135 127 140 138 133 157 C92457 159 84 89 C92459 167 153 142 126 89 86 C92460 147 134 80 107 58 SPILLED C92462 182 173 148 131 59 37 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 33 4084 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 9 - 1 PPM C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 0.097 0.103 0.101 0.102 0.098 0.101 0.090 0.101 0.113 SPILLED 0.095 0.102 0.112 0.104 0.098 0.100 0.100 0.098 0.107 0.102 0.092 0.104 0.103 0.109 0.105 0.092 0.101 0.103 0.105 0.097 0.101 0. 084 0. 089 0. 082 0. 088 0. 084 0. 086 0. 071 0. 090 0. 098 0. 111 0. 081 0. 083 0..092 0. 084 0. 080 0. 088 0. 090 0. 084 SPILLED 0. 089 0. 082 0. 086 0. 086 0. 084 0. 089 0. 078 0. 081 0. 086 0. 087 0. 085 0. 081 0. 076 0. 082 0. 073 0. 079 0. 078 0. 079 0. 066 0. 079 0. 088 0. 110 0. 074 0. 076 0. 084 0. 077 0. 073 SPILLED 0. 080 0. 075 0. 096 0. 083 0. 074 0. 074 0. 079 0. 075 0. 078 0. 075 0. 073 0. 075 0. 081 0. 077 0. 071 0. 070 0. 071 0. 066 NOT TAKEN 0. 070 0. 068 0. 058 0. 077 0. 076 SPILLED 0. 067 0. 069 0. 074 0. 068 0. 066 0. 076 0. 074 0. 065 0. 077 NOT TAKEN 0. 070 0. 071 0. 075 NOT TAKEN 0. 070 0. 070 0. 067 0. 069 0. 076 0. 068 0. 066 0. 062 0. 064 0. 062 NOT TAKEN 0. 065 0. 066 0. 053 0. 072 0. 072 SPILLED 0. 063 0. 064 0. 070 0. 064 0. 063 0. 069 0. 070 0. 062 0. 073 NOT TAKEN 0. 064 0. 065 0. 070 NOT TAKEN 0. 063 0. 066 0. 062 0. 063 0. 071 0. 065 0. 060 0.059 0.061 0.060 NOT TAKEN 0.065 0.060 0.051 0.067 0.067 SPILLED SPILLED 0.060 0.067 0.060 0.058 0.066 0.065 0.060 0.070 NOT TAKEN 0.064 0.059 0.066 NOT TAKEN 0.062 0.061 0.059 0.060 0.067 0.061 0.057 0. 057 0. 060 0. 058 NOT TAKEN 0. 053 0. 057 0. 050 0. 067 0. 068 0. 081 0. 057 0. 060 0. 064 0. 057 0. 059 0. 061 0. 061 0. 060 0. 066 NOT TAKEN 0. 058 0. 058 0. 065 NOT TAKEN 0. 058 0. 059 0. 055 0. 058 0. 065 0. 063 0. 054 0. 056 0. 059 0. 056 NOT TAKEN SPILLED 0. 055 0. 048 0. 062 SPILLED 0. 086 0. 056 0. 055 0. 057 0. 053 0. 057 0. 060 0. 058 0. 057 0. 061 NOT TAKEN 0. 057 0. 055 0. 059 NOT TAKEN 0. 057 0. 055 0. 053 0. 055 0. 063 0. 060 0. 052 0. 054 0. 058 0. 054 NOT TAKEN 0. 063 0. 053 0. 050 0. 059 0. 070 0. 068 0. 053 0. 055 0. 065 0. 055 0. 056 0. 054 0. 056 0. 056 0. 058 NOT TAKEN 0. 055 0. 054 0. 063 NOT TAKEN 0. 057 0. 053 0. 050 0. 055 0. 059 0. 061 0. 050 0.054 0.054 0.052 NOT TAKEN SPILLED 0.054 0.047 0.053 SPILLED 0.074 0.054 0.055 0.058 0.057 0.056 0.054 0.052 0.053 0.057 NOT TAKEN 0.054 0.054 0.059 NOT TAKEN 0.056 0.052 0.050 0.053 0.057 0.057 0.047 4085 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 9 - 1 PPM C92292 C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 0.097 0.106 0.093 0.114 0.100 0.102 0.099 0.107 0.097 0.108 0.101 0.103 0.104 0.107 0.105 0.121 0.101 0.106 0.102 0.109 0.093 0.097 0.090 0.105 0.104 0.094 0.114 0.108 0.112 0.111 0.117 0.081 0.094 0.076 0.109 0.085 0.086 0.081 0.087 0.086 0.087 0.087 0.086 0.086 0.090 0.091 0.093 0.085 0.090 0.087 0.089 0.080 0.081 0.078 0.091 0.106 0.081 0.096 0.095 0.097 0.094 0.093 0.076 0.037 0.067 0.089 0.076 0.078 0.074 0.080 0.077 0.081 0.076 0.080 0.078 0.078 0.080 0.086 0.076 0.080 0.077 0.080 0.076 0.072 0.072 0.078 SPILLED 0.072 0.086 SPILLED 0.085 0.080 0.078 0.071 0.074 0.063 0.076 0.068 0.071 0.068 0.076 NOT TAKEN 0.072 0.068 0.071 0.072 0.073 0.069 0.077 0.070 0.070 0.072 0.073 0.069 0.065 0.065 0.070 0.079 0.068 0.082 0.074 0.077 0.075 0.078 0.067 SPILLED 0.060 0.068 0.063 0.064 0.065 0.069 NOT TAKEN 0.069 0.064 0.068 0.066 0.069 0.070 0.074 0.064 0.066 0.066 0.069 0.066 0.064 0.065 0.072 SPILLED 0.064 SPILLED 0.070 0.072 0.069 0.068 0.064 0.067 0.059 0.062 0.058 0.060 0.060 0.068 NOT TAKEN 0.063 0.059 0.063 0.063 0.068 0.061 0.069 0.062 0.065 0.062 0.065 0.064 0.062 0.058 0.068 0.064 0.059 0.065 0.062 0.067 0.062 0.062 0.062 0.064 0.059 0.059 0.056 0.058 0.057 0.067 NOT TAKEN 0.055 0.056 0.059 0.060 0.063 0.060 0.067 0.062 0.063 0.060 0.061 0.066 0.063 0.059 0.068 0.057 0.057 0.064 0.063 0.065 0.064 0.059 0.058 0.060 0.061 0.059 0.053 0.059 0.053 SPILLED NOT TAKEN 0.056 0.056 0.055 0.057 0.055 0.060 0.064 0.059 0.068 0.062 0.061 0.060 0.057 0.056 0.062 0.055 0.055 0.068 0.055 0.060 0.058 0.057 0.057 0.058 0.062 0.055 0.053 0.059 0.052 SPILLED NOT TAKEN 0.055 0.053 0.054 0.058 0.057 0.057 0.061 0.058 0.060 0.053 0.056 0.060 0.058 0.054 0.060 0.051 0.054 0.058 0.055 0.059 0.060 0.055 0.058 0.058 0.058 0.054 0.053 0.060 0.049 0.061 NOT TAKEN 0.054 0.050 0.052 0.055 0.053 0.057 0.057 0.057 0.057 0.053 0.057 0.059 0.056 0.053 0.059 0.049 0.052 0.056 0.054 0.058 0.057 0.053 4086 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: MALE 9 - 1 PPM C92323 C92324 C92325 C92326 C92327 C92328 C92329 C92330 0.116 0.097 0.102 0.116 0.106 0.104 0.108 0.096 0.093 0.083 0.087 0.099 0.092 0.089 0.096 0.082 GROUP: FEMALE 9 - 1 PPM C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 0.107 0.108 0.097 0.113 0.126 0.091 0.109 0.101 0.131 0.095 0.112 0.103 SPILLED 0.090 0.116 0.138 SPILLED 0.102 SPILLED SPILLED 0.089 0.099 0.091 0.099 0.092 0.109 0.105 0.090 SPILLED 0.084 SPILLED SPILLED SPILLED 0.076 0.102 SPILLED SPILLED 0.089 SPILLED SPILLED 0.085 0.076 0.080 0.083 0.081 0.081 0.086 0.076 0.087 0.091 0.090 0.091 0.083 0.086 0.092 0.082 0.087 0.076 0.129 0.115 0.088 0.073 0.098 0.101 0.092 0.084 0.089 SPILLED 0.076 0.071 0.072 0.075 0.076 0.077 NOT TAKEN 0.072 0.082 NOT TAKEN 0.079 0.094 0.080 0.076 0.093 0.081 0.081 0.071 SPILLED SPILLED 0.083 0.081 0.094 0.087 0.099 0.082 0.095 SPILLED 0.067 0.070 0.065 0.067 0.072 SPILLED NOT TAKEN 0.064 0.081 NOT TAKEN 0.080 0.083 0.076 SPILLED 0.081 0.076 0.084 0.071 SPILLED SPILLED 0.078 0.073 0.087 0.083 0.086 0.073 SPILLED SPILLED 0.063 0.061 0.063 0.062 0.065 SPILLED NOT TAKEN 0.062 0.074 NOT TAKEN 0.076 0.076 0.092 0.090 0.077 0.069 0.090 0.064 SPILLED 0.080 0.073 0.067 0.079 0.073 0.070 0.066 SPILLED SPILLED 0.059 0.064 0.063 0.059 0.061 SPILLED NOT TAKEN 0.059 0.072 NOT TAKEN 0.070 0.076 0.082 0.077 0.071 0.068 0.079 0.066 0.072 0.090 0.074 0.069 0.073 0.070 0.071 0.060 SPILLED 0.081 0.059 0.059 0.056 0.057 0.056 SPILLED NOT TAKEN 0.055 0.067 NOT TAKEN 0.062 0.070 SPILLED 0.073 0.067 0.069 SPILLED 0.067 SPILLED SPILLED 0.069 0.069 0.077 0.067 0.073 0.060 SPILLED SPILLED 0.056 0.056 0.055 0.057 0.053 0.064 NOT TAKEN 0.054 0.067 NOT TAKEN 0.061 0.072 0.063 0.072 0.062 0.064 0.069 0.060 SPILLED SPILLED 0.074 0.067 0.072 0.066 0.067 0.059 0.081 0.077 0.054 0.056 0.054 0.053 0.053 0.059 NOT TAKEN 0.054 0.063 NOT TAKEN 0.067 0.069 0.077 0.070 0.064 0.059 0.072 0.063 SPILLED 0.061 SPILLED 0.069 0.067 0.068 0.065 0.056 SPILLED SPILLED 4087 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 9 - 1 PPM C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 0.099 0.098 SPILLED SPILLED 0.123 0.113 0.093 0.094 SPILLED 0.107 0.101 0.102 SPILLED 0.110 0.108 0.106 0.096 0.111 0.102 SPILLED 0.095 0.110 0.109 SPILLED 0.109 0.113 0.099 0.119 SPILLED 0.102 0.098 0.088 0.096 SPILLED 0.121 0.100 0.107 0.102 0.087 SPILLED 0.091 0.089 0.088 SPILLED 0.086 0.094 0.086 0.079 0.092 0.089 0.124 0.097 0.099 0.092 0.104 0.101 0.098 0.093 0.102 0.101 0.087 0.091 0.080 0.080 0.144 0.118 0.095 0.098 0.077 0.088 0.091 0.104 0.082 0.084 0.126 0.089 0.081 0.087 0.078 0.086 0.079 0.111 0.082 0.097 0.088 0.103 0.089 0.103 0.107 0.087 0.094 0.086 0.080 0.075 0.076 SPILLED SPILLED 0.089 0.086 0.082 0.078 0.089 0.082 0.078 0.081 0.131 0.075 NOT TAKEN 0.081 0.073 0.082 0.079 0.098 0.079 0.091 0.083 SPILLED 0.085 0.091 0.086 0.090 0.091 0.078 0.085 0.073 0.077 SPILLED SPILLED 0.085 0.076 SPILLED 0.073 0.083 0.086 0.075 0.077 SPILLED 0.077 NOT TAKEN 0.080 0.076 0.080 0.074 0.080 0.078 0.083 0.081 0.088 0.080 0.082 0.080 0.080 0.081 0.082 0.079 0.082 0.075 0.080 SPILLED 0.076 0.086 SPILLED 0.072 0.086 0.071 0.074 0.070 SPILLED 0.075 NOT TAKEN 0.073 0.073 0.075 0.068 0.076 0.071 0.080 0.081 SPILLED 0.074 SPILLED 0.075 0.072 SPILLED 0.070 0.074 0. 068 0. 069 0. 087 SPILLED 0. 072 0. 074 0. 088 0. 071 0. 073 0. 075 0. 071 0. 068 SPILLED 0. 071 NOT TAKEN 0. 073 0. 067 0. 069 0. 068 0. 072 0. 069 0. 083 0. 075 0. 088 0. 070 0. 080 NOT TAKEN 0. 069 0. 099 0. 068 0. 064 SPILLED 0.066 SPILLED 0.089 0.077 0.074 0.123 SPILLED SPILLED 0.068 0.071 0.073 SPILLED 0.073 NOT TAKEN 0.065 0.067 0.068 0.073 0.071 0.076 0.073 0.073 SPILLED 0.064 0.068 EXCLUDE 0.068 SPILLED 0.066 0.063 SPILLED 0.066 SPILLED 0.082 0.069 SPILLED 0.070 0.064 0.068 0.065 0.072 0.064 0.068 0.067 NOT TAKEN 0.066 0.063 0.063 0.061 0.072 0.060 0.068 0.061 SPILLED 0.061 0.063 0.067 0.066 0.078 0.061 0.067 SPILLED 0.066 SPILLED SPILLED 0.069 0.065 0.069 0.063 0.069 0.064 0.066 0.065 SPILLED 0.070 NOT TAKEN 0.063 0.067 0.060 0.060 0.065 0.062 0.068 0.069 SPILLED 0.065 0.073 SPILLED 0.065 SPILLED 0.065 0.069 4088 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 5 ANIMAL NUMBER WEEK 1 WEEK 2 WEEK 3 WEEK 4 WEEK 5 WEEK 6 WEEK 7 WEEK 8 WEEK 9 WEEK 10 GROUP: FEMALE 9 - 1 PPM C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 0.113 0.104 0.119 0.107 SPILLED 0.110 0.109 0.109 0.126 0.122 0.106 0.106 0.114 0.114 0.111 0.106 0.116 SPILLED 0.110 SPILLED 0.087 0.104 0.091 SPILLED 0.090 0.100 0.098 SPILLED 0.112 SPILLED 0.096 0.097 0.103 0.097 0.088 0.092 0.112 0.104 SPILLED 0.091 0.093 0.089 SPILLED 0.088 0.095 0.089 SPILLED 0.087 0.095 0.088 0.088 0.099 0.091 0.098 0.088 0.108 0.091 SPILLED 0.081 0.090 0.086 0.141 0.080 0.089 0.086 0.092 0.086 0.087 NOT TAKEN 0.089 0.092 0.084 NOT TAKEN 0.076 0.091 NOT TAKEN SPILLED 0.080 0.081 0.081 SPILLED 0.083 0.083 0.081 0.097 SPILLED 0.080 NOT TAKEN 0.081 0.085 0.079 NOT TAKEN 0.076 0.086 NOT TAKEN SPILLED SPILLED 0.086 0.072 SPILLED 0.072 0.084 0.077 0.092 0.082 SPILLED NOT TAKEN 0.081 0.087 0.077 NOT TAKEN 0.074 0.096 NOT TAKEN SPILLED SPILLED SPILLED SPILLED SPILLED 0.064 0.070 0.075 0.082 0.071 0.087 NOT TAKEN 0.082 0.076 0.072 NOT TAKEN 0.077 0.095 NOT TAKEN SPILLED 0.073 0.071 0.071 SPILLED SPILLED 0.074 0.075 0.092 SPILLED 0.078 NOT TAKEN 0.070 0.076 0.078 NOT TAKEN 0.074 0.075 NOT TAKEN 0.084 0.071 0.069 0.065 0.080 0.072 0.071 0.071 0.122 SPILLED 0.080 NOT TAKEN 0.071 0.070 0.075 NOT TAKEN 0.071 0.073 NOT TAKEN 0.079 0.074 0.071 0.071 SPILLED 0.064 0.076 0.070 SPILLED 0.076 0.075 NOT TAKEN 0.073 SPILLED 0.069 NOT TAKEN 0.129 0.072 NOT TAKEN 4089 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 9 - 1 PPM C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 0. 050 0. 052 0. 049 NOT TAKEN SPILLED 0. 053 0. 047 0. 051 SPILLED 0. 057 0. 049 0. 052 0. 054 0. 053 0. 053 0. 052 0. 052 0. 049 0. 054 NOT TAKEN 0. 050 0. 053 SPILLED NOT TAKEN 0. 054 0. 049 0. 048 0. 051 0. 055 0. 056 0. 047 0. 052 0. 053 0. 049 NOT TAKEN 0. 060 0. 053 0. 047 0. 051 SPILLED 0. 067 0. 052 0. 052 0. 053 0. 052 0. 053 0. 052 0. 051 0. 051 0. 053 NOT TAKEN 0. 048 0. 051 0. 056 NOT TAKEN 0. 050 0. 050 0. 049 0. 052 0. 053 0. 054 0. 048 0. 050 0. 051 0. 049 NOT TAKEN 0. 059 0. 052 0. 047 0. 052 SPILLED 0. 056 0. 052 0. 055 0. 054 0. 051 0. 051 0. 052 0. 051 0. 050 0. 055 NOT TAKEN 0. 051 0. 051 0. 057 NOT TAKEN 0. 052 0. 049 0. 050 0. 050 0. 054 0. 056 0. 048 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.052 0.049 0.046 0.049 0.052 0.048 0.047 0.051 NOT TAKEN 0.048 0.049 0.052 NOT TAKEN 0.050 0.045 0.047 0.048 0.051 0.053 0.049 0. 050 0. 049 0. 049 NOT TAKEN 0. 057 0. 050 0. 045 SPILLED SPILLED 0. 060 0. 047 0. 050 0. 051 0. 046 0. 049 0. 050 0. 046 0. 048 0. 051 NOT TAKEN 0. 050 0. 049 0. 052 NOT TAKEN 0. 049 0. 044 0. 048 0. 047 0. 051 0. 053 0. 048 0. 050 0. 050 0. 048 NOT TAKEN 0. 054 0. 049 0. 044 0. 049 0. 047 0. 054 0. 046 0. 050 0. 052 0. 046 0. 047 0. 049 0. 045 0. 046 0. 049 NOT TAKEN 0. 047 0. 048 0. 050 NOT TAKEN 0. 048 0. 047 0. 047 0. 048 0. 050 0. 050 0. 045 0.049 0.045 0.044 NOT TAKEN 0.048 0.039 0.042 0.044 0.050 0.047 0.045 0.047 SPILLED 0.044 0.046 0.046 0.041 0.041 0.043 NOT TAKEN 0.044 0.047 0.044 NOT TAKEN 0.042 0.041 0.044 0.044 0.046 0.042 0.043 0. 047 0. 043 0. 042 NOT TAKEN 0. 044 0. 044 0. 042 0. 042 0. 034 0. 045 0. 043 0. 049 0. 049 0. 043 0. 044 0. 045 0. 038 0. 041 0. 045 NOT TAKEN 0. 043 0. 049 0. 044 NOT TAKEN 0. 040 0. 039 0. 043 0. 042 0. 044 0. 043 0. 045 0.048 0.046 0.041 NOT TAKEN 0.050 0.046 0.042 0.043 SPILLED 0.049 0.043 SPILLED SPILLED 0.042 0.044 0.047 0.041 0.040 0.043 NOT TAKEN 0.044 0.048 0.044 NOT TAKEN 0.040 0.039 0.041 0.043 0.044 0.041 0.045 0. 048 0. 042 0. 040 NOT TAKEN 0. 048 0. 043 0. 039 0. 041 0. 045 0. 045 0. 041 0. 045 0. 046 0. 042 0. 043 0. 044 0. 040 0. 040 0. 041 NOT TAKEN 0. 043 0. 049 0. 040 NOT TAKEN 0. 039 0. 036 0. 043 0. 039 0. 044 0. 041 0. 044 4090 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 9 - 1 PPM C92292 C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 0. 058 0. 056 0. 055 0. 053 0. 051 0. 057 0. 048 0. 060 NOT TAKEN 0. 052 0. 050 0. 050 0. 050 0. 051 0. 056 0. 056 0. 054 0. 055 0. 050 0. 052 0. 055 0. 054 0. 052 0. 058 0. 049 0. 051 0. 056 0. 054 0. 058 0. 056 0. 056 0. 057 0. 061 0. 052 0. 052 0. 052 0. 058 0. 049 0. 062 NOT TAKEN 0. 054 0. 050 0. 050 0. 052 0. 053 0. 056 0. 057 0. 056 0. 056 0. 051 0. 052 0. 056 0. 054 0. 052 0. 057 0. 051 0. 048 0. 057 0. 056 0. 056 0. 057 0. 055 0. 056 0. 057 0. 053 0. 049 0. 051 0. 056 0. 051 0. 060 NOT TAKEN 0. 052 0. 049 0. 051 0. 051 0. 054 0. 056 0. 055 0. 058 0. 053 0. 052 0. 052 0. 056 0. 055 0. 052 0. 054 0. 047 0. 048 0. 056 0. 054 0. 052 0. 055 0. 053 0. 054 0. 055 0. 049 0. 047 0. 049 0. 058 0. 047 0. 054 NOT TAKEN 0. 052 0. 045 0. 049 0. 050 0. 053 0. 054 0. 054 0. 051 0. 050 0. 049 0. 049 0. 054 0. 053 0. 049 0. 054 0. 050 0. 048 0. 053 0. 053 0. 054 0. 053 0. 051 0. 052 0. 051 0. 052 0. 051 0. 048 0. 055 0. 047 0. 056 NOT TAKEN 0. 053 0. 044 0. 048 0. 048 0. 052 0. 054 0. 052 0. 050 0. 050 0. 051 0. 050 0. 054 0. 054 0. 051 0. 055 0. 048 0. 048 0. 051 0. 051 0. 052 0. 055 0. 050 0. 052 0. 052 0. 050 0. 048 0. 047 0. 053 0. 045 0. 055 NOT TAKEN 0. 050 0. 045 0. 049 0. 047 0. 051 0. 053 0. 052 0. 049 0. 049 0. 050 0. 049 0. 053 0. 051 0. 051 0. 054 0. 047 0. 046 0. 055 0. 045 0. 052 0. 054 0. 049 0. 049 0. 047 0. 042 0. 041 0. 046 0. 053 0. 039 SPILLED NOT TAKEN 0. 047 0. 041 0. 045 0. 046 0. 043 0. 046 0. 046 SPILLED 0. 046 0. 043 0. 046 0. 052 0. 047 0. 046 0. 048 0. 041 0. 041 0. 049 0. 052 0. 048 0. 049 0. 048 0. 049 0. 047 0. 042 0. 044 0. 044 0. 054 0. 043 0. 050 NOT TAKEN 0. 049 0. 041 0. 045 0. 044 0. 042 0. 045 0. 048 0. 051 0. 046 0. 039 0. 045 0. 051 0. 044 0. 045 0. 045 0. 041 0. 040 0. 047 0. 049 0. 046 0. 050 0. 047 0. 046 0. 047 0. 042 0. 043 0. 044 0. 054 0. 040 SPILLED NOT TAKEN 0. 045 0. 037 0. 046 0. 046 0. 043 0. 041 0. 048 0. 049 0. 047 0. 039 0. 040 0. 050 0. 045 0. 045 0. 042 0. 042 0. 040 0. 045 0. 053 0. 045 0. 049 0. 047 0. 045 0. 045 0. 042 0. 042 0. 044 0. 054 0. 040 0. 049 NOT TAKEN 0. 045 0. 041 0. 044 0. 046 0. 041 0. 042 0. 046 0. 053 0. 046 0. 037 0. 042 0. 046 0. 043 0. 045 0. 042 0. 040 0. 038 0. 043 0. 048 0. 043 0. 046 0. 043 4091 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: MALE 9 - 1 PPM C92323 C92324 C92325 C92326 C92327 C92328 C92329 C92330 0.053 0.053 0.052 0.052 0.052 SPILLED NOT TAKEN 0.051 0.053 0.056 0.052 0.057 0.051 0.055 NOT TAKEN 0.050 GROUP: FEMALE 9 - 1 PPM C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 0.062 NOT TAKEN 0.057 0.063 SPILLED 0.063 0.059 0.060 0.061 0.057 SPILLED SPILLED 0.060 0.057 0.060 0.054 0.062 0.056 SPILLED 0.065 0.061 NOT TAKEN 0.070 0.065 0.063 SPILLED 0.059 0.061 0.063 0.061 SPILLED SPILLED 0.059 0.062 0.064 0.062 0.064 0.059 0.074 SPILLED 0. 054 0. 052 0. 051 0. 052 0. 049 0. 053 NOT TAKEN 0. 050 0. 061 NOT TAKEN 0. 063 0. 064 0. 069 0. 062 0. 057 0. 058 0. 062 0. 060 0. 096 0. 073 0. 065 0. 061 0. 055 0. 063 0. 060 0. 060 SPILLED 0. 078 0.051 0.053 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.075 0.059 0.063 SPILLED 0.056 0.053 0.093 0.079 0.050 0.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.058 NOT TAKEN 0.056 0.062 0.071 0.067 0.060 0.057 0.065 0.058 0.069 0.062 0.068 0.056 0.059 SPILLED 0.056 0.054 SPILLED 0.083 0.050 0.053 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.060 NOT TAKEN 0.056 0.065 0.061 0.068 0.058 0.059 0.059 0.056 SPILLED SPILLED 0.069 0.056 0.056 0.065 0.058 0.052 SPILLED SPILLED 0.045 0.048 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.055 NOT TAKEN 0.054 0.060 0.056 0.067 0.060 0.055 0.057 0.053 SPILLED SPILLED 0.060 0.057 0.059 0.068 0.051 0.061 SPILLED SPILLED 0. 044 0. 047 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 054 NOT TAKEN 0. 052 0. 060 0. 058 0. 071 0. 055 0. 054 0. 062 0. 055 0. 081 0. 056 0. 059 0. 054 0. 052 0. 060 0. 056 0. 052 SPILLED SPILLED 0. 046 0. 048 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0. 057 NOT TAKEN 0. 054 0. 055 0. 067 0. 072 0. 061 0. 059 0. 063 0. 053 0. 076 0. 061 0. 057 0. 057 0. 057 0. 063 0. 059 0. 050 SPILLED 0..092 0.043 0.045 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.054 NOT TAKEN 0.048 0.058 0.061 0.067 0.054 0.053 0.057 0.057 0.068 0.071 0.048 0.052 0.056 0.063 0.052 0.050 0.069 0.081 4092 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 9 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 9 - 1 PPM C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 SPILLED 0.058 SPILLED SPILLED 0.059 SPILLED SPILLED 0.060 0.069 0.060 0.055 0.057 0.055 0.063 NOT TAKEN 0.065 0.068 0.058 0.057 0.059 0.061 0.056 0.062 SPILLED 0.054 0.061 0.065 0.060 SPILLED 0.059 0.065 0.066 0.059 0.073 0.082 0.062 0.068 0.080 0.063 0.069 0.068 0.056 0.064 0.059 0.063 NOT TAKEN 0.062 0.067 0.060 0.061 0.064 0.062 0.056 0.058 SPILLED 0.056 SPILLED SPILLED 0.065 SPILLED 0.065 0.070 0.063 0.060 0.082 0.078 0.065 0.071 SPILLED 0.060 0.063 0.068 0.064 0.060 0.056 0.067 NOT TAKEN 0.065 0.071 0.062 0.058 0.071 0.060 0.062 0.065 SPILLED 0.061 0.063 0.065 0.068 0.067 0.071 0.066 0.059 0.061 SPILLED SPILLED 0.062 SPILLED SPILLED 0.053 SPILLED 0.060 0.062 0.064 SPILLED 0.070 NOT TAKEN 0.059 0.063 0.060 0.059 0.061 SPILLED 0.062 0.057 SPILLED 0.056 0.051 0.061 0.068 0.082 0.060 0.068 0.059 0.058 SPILLED 0.087 0.058 SPILLED SPILLED 0.056 0.061 0.058 0.054 0.057 0.055 0.065 NOT TAKEN 0.060 0.058 0.057 0.060 0.068 0.056 0.056 0.064 SPILLED 0.054 0.061 0.067 0.062 0.079 0.059 0.064 0.055 0.054 SPILLED SPILLED 0.058 SPILLED SPILLED 0.059 0.064 0.059 0.050 0.061 0.060 0.070 NOT TAKEN 0.063 0.059 0.060 0.063 0.063 0.058 0.055 0.056 SPILLED 0.052 0.061 SPILLED 0.063 0.069 0.063 0.072 0.055 0.055 0.071 0.105 0.057 0.117 SPILLED 0.055 0.061 0.058 0.053 0.062 0.076 0.066 NOT TAKEN 0.056 0.062 0.059 0.063 0.056 0.066 0.053 0.059 SPILLED 0.049 0.056 0.066 0.057 0.060 0.057 0.071 0.059 0.057 SPILLED SPILLED 0.059 SPILLED 0.060 0.055 0.061 0.056 0.058 0.062 0.055 0.060 NOT TAKEN 0.057 0.064 0.061 0.057 0.061 0.062 0.058 0.054 0.085 0.049 0.054 0.063 0.054 0.060 0.056 0.062 0. 061 0. 060 0. 073 0. 086 0. 054 0. 075 0. 059 0. 053 0. 064 0. 057 0. 061 0. 063 0. 058 0. 067 NOT TAKEN 0. 068 0. 076 0. 062 0. 055 0. 064 0. 060 0. 053 0. 055 SPILLED 0. 046 0. 060 0. 067 0. 055 0. 064 0. 057 0. 063 0. 061 0. 056 0. 067 0. 085 0. 059 0. 068 0. 067 0. 044 0. 075 0. 055 0. 058 0. 059 0. 057 0. 063 NOT TAKEN 0. 055 0. 068 0. 061 0. 051 0. 057 0. 059 0. 050 0. 054 0. 087 0. 044 0. 061 0. 062 0. 055 0. 063 0. 053 0. 060 4093 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 10 ANIMAL NUMBER WEEK 11 WEEK 12 WEEK 13 WEEK 14 WEEK 15 WEEK 16 WEEK 20 WEEK 24 WEEK 28 WEEK 32 GROUP: FEMALE 9 - 1 PPM C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 0.075 0.076 0.068 0.075 0.073 0.061 0.069 0.061 SPILLED 0.074 0.090 NOT TAKEN 0.070 0.067 SPILLED NOT TAKEN SPILLED 0.069 NOT TAKEN 0.078 0.070 0.075 0.061 SPILLED 0.065 0.070 0.069 SPILLED 0.074 0.077 NOT TAKEN 0.075 0.072 0.072 NOT TAKEN 0.068 0.066 NOT TAKEN 0.081 0.068 0.069 0.064 SPILLED 0.067 0.069 0.072 0.105 0.079 0.088 NOT TAKEN 0.073 0.065 0.077 NOT TAKEN 0.063 0.070 NOT TAKEN 0.081 0.069 0.071 0.068 0.069 0.063 0.058 0.070 SPILLED SPILLED 0.075 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.080 0.066 0.066 0.062 0.068 0.061 0.064 0.075 0.126 0.077 0.078 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.079 0.063 0.064 0.064 0.074 0.062 0.069 0.071 SPILLED SPILLED 0.077 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.055 0.053 0.056 0.081 0.063 0.063 0.069 SPILLED 0.069 0.076 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.067 0.051 0.053 0.052 0.057 0.057 0.059 0.067 0.100 0.070 0.070 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.069 0.058 0.061 0.058 0.061 0.059 0.059 0.060 SPILLED 0.074 0.073 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.054 0.057 0.056 0.058 0.066 0.056 0.037 0.081 0.069 0.064 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4094 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 9 - 1 PPM C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 0.047 0.041 0.038 NOT TAKEN 0.045 0.038 0.039 0.037 0.042 0.043 0.040 SPILLED 0.039 0.040 0.042 0.042 0.038 0.038 0.040 NOT TAKEN 0.041 0.046 0.040 NOT TAKEN 0.037 0.035 0.039 0.038 0.041 0.038 0.043 0. 044 0. 040 0. 036 NOT TAKEN 0. 045 0. 038 0. 038 0. 037 0. 040 0. 040 0. 038 0. 040 0. 038 0. 039 0. 039 0. 042 0. 036 0. 037 0. 039 NOT TAKEN 0. 039 0. 042 0. 038 NOT TAKEN 0. 035 0. 034 0. 038 0. 037 0. 039 0. 037 0. 041 0.042 0.037 0.036 NOT TAKEN 0.049 0.037 0.037 0.035 0.038 0.040 0.035 0.039 NOT TAKEN 0.033 0.039 0.040 0.035 0.034 0.035 NOT TAKEN 0.037 0.043 0.037 NOT TAKEN 0.034 0.032 0.036 0.035 0.037 0.034 0.038 0.040 0.038 0.035 NOT TAKEN 0.043 0.036 0.036 0.035 0.036 0.039 0.035 0.037 NOT TAKEN 0.036 0.039 0.038 0.035 0.036 0.034 NOT TAKEN 0.038 0.041 0.036 NOT TAKEN 0.034 0.032 0.037 0.036 0.036 0.033 0.040 0.037 0.036 0.033 NOT TAKEN 0.039 0.035 0.034 0.032 0.036 0.037 0.034 0.035 NOT TAKEN 0.035 0.036 0.041 0.034 0.033 0.035 NOT TAKEN 0.036 0.037 0.036 NOT TAKEN 0.031 0.031 0.033 0.034 0.036 0.031 0.038 0.038 0.036 0.032 NOT TAKEN 0.038 0.035 NOT TAKEN 0.031 0.034 0.036 0.033 0.035 NOT TAKEN NOT TAKEN 0.036 0.040 0.029 0.034 0.034 NOT TAKEN 0.033 0.038 0.035 NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN 0.038 0.017 0.035 0.038 0.035 0.035 NOT TAKEN 0.037 0.035 NOT TAKEN 0.031 0.035 0.036 0.034 0.038 NOT TAKEN NOT TAKEN 0.037 0.040 NOT TAKEN 0.034 0.036 NOT TAKEN 0.035 0.038 0.033 NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN 0.042 0.017 0.039 0.038 0.036 0.034 NOT TAKEN 0.038 0.035 NOT TAKEN 0.033 0.036 0.035 0.035 0.036 NOT TAKEN NOT TAKEN 0.037 0.042 NOT TAKEN 0.034 0.035 NOT TAKEN 0.037 0.040 0.034 NOT TAKEN 0.029 NOT TAKEN NOT TAKEN NOT TAKEN 0.038 NOT TAKEN 0.040 0.037 0.036 0.033 NOT TAKEN 0.038 0.035 NOT TAKEN 0.031 0.036 0.037 0.033 0.038 NOT TAKEN NOT TAKEN 0.038 0.042 NOT TAKEN 0.032 0.035 NOT TAKEN 0.037 0.033 0.035 NOT TAKEN 0.032 NOT TAKEN NOT TAKEN NOT TAKEN 0.037 NOT TAKEN 0.040 0.035 0.034 0.034 NOT TAKEN 0.037 0.035 NOT TAKEN 0.031 0.034 0.036 0.033 0.034 NOT TAKEN NOT TAKEN 0.037 0.013 NOT TAKEN 0.036 0.037 NOT TAKEN 0.037 0.035 0.034 NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN 0.035 NOT TAKEN 0.040 4095 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 9 - 1 PPM C92292 C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 0.043 0.043 0.040 0.039 0.041 SPILLED 0.036 0.047 NOT TAKEN 0.042 0.037 SPILLED 0.042 0.040 0.038 0.041 0.053 0.043 0.037 SPILLED 0.045 0.042 0.041 0.040 0.038 0.038 0.039 0.047 0.042 0.044 0.042 0. 041 0. 042 0. 038 0. 036 0. 039 0. 046 0. 036 0. 046 NOT TAKEN 0. 038 0. 035 0. 039 0. 041 0. 038 0. 038 0. 042 0. 053 0. 041 0. 036 0. 039 0. 042 0. 041 0. 040 0. 039 0. 036 0. 037 0. 038 0. 043 0. 040 0. 042 0. 039 0. 037 NOT TAKEN 0. 040 0. 037 0. 038 0. 046 0. 039 0. 044 NOT TAKEN 0. 037 0. 036 0. 039 0. 038 0. 036 0. 036 0. 040 0. 044 0. 040 0. 035 0. 035 0. 041 0. 038 0. 038 0. 037 0. 035 0. 035 0. 037 0. 041 0. 040 0. 040 0. 038 0. 035 NOT TAKEN 0. 035 0. 038 0. 037 0. 044 0. 036 0. 042 NOT TAKEN 0. 036 0. 033 0. 037 0. 038 0. 035 0. 037 0. 039 0. 048 0. 040 0. 034 0. 035 0. 041 0. 038 0. 037 0. 035 0. 034 0. 034 0. 035 0. 043 0. 037 0. 037 0. 036 0. 035 NOT TAKEN 0. 033 0. 038 0. 033 0. 043 0. 034 SPILLED NOT TAKEN 0. 036 0. 032 0. 036 0. 036 0. 036 0. 035 0. 037 0. 045 0. 036 0. 034 0. 034 0. 040 0. 036 0. 037 0. 037 0. 035 0. 032 0. 033 0. 038 0. 037 0. 040 0. 034 0.026 NOT TAKEN 0.034 0.036 0.032 0.039 0.033 SPILLED NOT TAKEN 0.037 0.030 NOT TAKEN 0.035 0.037 0.034 0.041 0.056 0.035 0.030 0.034 0.043 0.035 0.035 NOT TAKEN 0.033 NOT TAKEN 0.034 0.038 NOT TAKEN 0.035 0.034 0.022 NOT TAKEN 0.035 0.037 0.032 0.039 0.034 SPILLED NOT TAKEN 0.037 0.033 NOT TAKEN 0.035 0.038 0.034 0.039 SPILLED 0.035 0.034 0.036 0.042 0.035 0.035 NOT TAKEN 0.033 NOT TAKEN 0.033 0.039 NOT TAKEN 0.036 0.035 0.022 NOT TAKEN 0.032 0.032 0.034 0.042 0.015 0.042 NOT TAKEN 0.037 0.022 NOT TAKEN 0.037 NOT TAKEN 0.034 0.042 SPILLED 0.038 0.036 0.035 0.042 0.038 0.037 NOT TAKEN 0.034 NOT TAKEN 0.034 0.039 NOT TAKEN 0.039 0.037 NOT TAKEN NOT TAKEN 0.025 0.033 0.032 0.043 NOT TAKEN 0.044 NOT TAKEN 0.035 0.032 NOT TAKEN 0.035 NOT TAKEN 0.034 0.043 0.054 0.038 0.036 0.034 NOT TAKEN 0.037 0.036 NOT TAKEN 0.033 NOT TAKEN 0.034 SPILLED NOT TAKEN 0.038 0.035 NOT TAKEN NOT TAKEN 0.029 0.036 0.035 0.043 NOT TAKEN 0.044 NOT TAKEN 0.036 0.032 NOT TAKEN 0.037 NOT TAKEN 0.039 0.043 SPILLED 0.038 0.021 0.035 NOT TAKEN 0.039 0.035 NOT TAKEN 0.035 NOT TAKEN 0.034 0.040 NOT TAKEN 0.040 0.035 4096 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 13 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: MALE 9 - 1 PPM C92323 C92324 C92325 C92326 C92327 C92328 C92329 C92330 0.039 0.045 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.038 0.041 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 9 - 1 PPM C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 0.047 NOT TAKEN 0.049 0.054 0.058 0.069 0.049 0.057 0.062 0.055 0.064 0.050 0.048 0.049 0.053 0.060 0.053 0.045 0.056 0.067 0.045 NOT TAKEN 0.040 0.053 0.050 0.064 0.048 0.051 0.054 0.051 0.060 0.053 0.040 0.046 0.044 0.053 0.042 0.044 0.057 0.066 0.036 0.041 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.047 NOT TAKEN 0.041 0.044 0.053 0.054 0.050 0.055 0.055 0.048 0.058 0.057 0.044 0.043 0.048 0.054 0.050 0.045 0.053 0.058 0.036 0.046 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.043 NOT TAKEN 0.028 0.045 0.051 0.058 0.039 0.050 0.050 0.045 0.076 0.050 0.045 0.044 0.047 0.048 0.046 0.042 0.049 0.060 0.033 0.042 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.045 NOT TAKEN NOT TAKEN 0.043 0.054 0.059 0.048 0.053 0.053 0.049 0.059 SPILLED 0.049 0.040 0.042 0.050 0.046 0.041 0.049 SPILLED 0.033 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.038 NOT TAKEN NOT TAKEN 0.041 0.050 NOT TAKEN NOT TAKEN 0.046 0.048 0.048 NOT TAKEN 0.050 0.034 0.044 0.044 0.041 0.040 NOT TAKEN 0.050 0.054 0.033 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.043 NOT TAKEN NOT TAKEN 0.044 0.046 NOT TAKEN NOT TAKEN 0.044 0.052 0.047 NOT TAKEN SPILLED 0.046 0.043 0.046 0.046 0.042 NOT TAKEN 0.053 0.050 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.039 NOT TAKEN NOT TAKEN 0.041 0.049 NOT TAKEN NOT TAKEN 0.044 0.050 0.048 NOT TAKEN 0.049 0.053 NOT TAKEN 0.043 0.045 0.040 NOT TAKEN 0.079 0.051 0.036 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.042 NOT TAKEN NOT TAKEN 0.046 0.047 NOT TAKEN NOT TAKEN 0.049 0.047 0.048 NOT TAKEN 0.052 0.040 NOT TAKEN 0.042 0.053 0.045 NOT TAKEN 0.048 0.043 0.034 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.040 NOT TAKEN NOT TAKEN 0.042 0.053 NOT TAKEN NOT TAKEN 0.046 0.054 0.048 NOT TAKEN 0.048 0.049 NOT TAKEN 0.037 0.048 0.045 NOT TAKEN 0.057 0.042 4097 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 9 - 1 PPM C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 0.061 0.051 SPILLED SPILLED 0.057 0.061 SPILLED 0.055 0.057 0.055 0.059 0.060 0.057 0.061 NOT TAKEN 0.057 0.064 0.052 SPILLED 0.059 SPILLED 0.047 0.049 0.069 0.042 0.049 0.055 0.045 0.064 0.049 SPILLED 0.055 0.050 0.068 0.076 0.048 0.058 SPILLED 0.047 0.057 0.051 0.046 0.055 0.053 0.057 NOT TAKEN 0.053 0.057 0.055 0.047 0.053 0.057 0.045 0.042 SPILLED 0.040 0.054 0.054 0.049 0.064 0.046 0.051 0. 051 0. 046 0. 055 SPILLED 0. 047 0. 061 0. 064 0. 046 0. 051 0. 054 0. 053 0. 055 0. 049 0. 054 NOT TAKEN 0. 057 0. 054 0. 054 0. 050 0. 051 0. 058 0. 044 0. 048 SPILLED 0. 040 0. 044 0. 056 0. 049 0. 062 0. 047 0. 045 0. 052 0. 048 0. 059 0. 083 0. 046 0. 058 0. 057 0. 048 0. 047 0. 050 0. 044 0. 052 0. 050 0. 053 NOT TAKEN 0. 055 0. 034 0. 050 0. 045 0. 061 0. 052 0. 042 0. 044 SPILLED 0. 037 0. 044 0. 052 0. 044 0. 062 0. 042 0. 043 0.045 SPILLED SPILLED SPILLED 0.045 0.060 0.082 0.040 0.044 0.045 0.038 0.055 0.047 0.055 NOT TAKEN 0.053 NOT TAKEN SPILLED 0.041 0.045 0.053 0.042 0.038 0.059 0.040 0.044 0.051 0.037 0.070 0.045 SPILLED 0.049 NOT TAKEN 0.049 NOT TAKEN 0.044 0.052 0.049 0.042 0.030 0.047 0.047 0.051 NOT TAKEN 0.052 NOT TAKEN 0.048 NOT TAKEN NOT TAKEN 0.047 0.048 NOT TAKEN 0.037 0.044 SPILLED 0.036 0.047 SPILLED 0.042 NOT TAKEN 0.041 SPILLED 0.056 NOT TAKEN 0.048 NOT TAKEN 0.046 0.051 SPILLED 0.026 0.043 0.046 0.041 0.050 NOT TAKEN 0.054 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN 0.041 0.076 NOT TAKEN 0.037 0.045 SPILLED 0.037 0.039 0.048 0.040 NOT TAKEN 0.041 0.056 0.048 NOT TAKEN 0.045 NOT TAKEN 0.040 0.050 NOT TAKEN NOT TAKEN NOT TAKEN 0.044 0.046 0.051 NOT TAKEN 0.056 NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 0.045 0.044 NOT TAKEN 0.019 0.040 0.057 0.035 0.040 0.043 0.018 NOT TAKEN 0.042 0.057 0.053 NOT TAKEN 0.051 NOT TAKEN 0.044 0.051 NOT TAKEN NOT TAKEN NOT TAKEN 0.049 0.048 0.051 NOT TAKEN 0.051 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN 0.048 0.048 NOT TAKEN NOT TAKEN 0.037 0.053 0.033 0.041 0.044 0.028 NOT TAKEN 0.045 0.058 0.050 NOT TAKEN 0.049 NOT TAKEN 0.042 0.050 NOT TAKEN NOT TAKEN NOT TAKEN 0.045 0.038 0.030 NOT TAKEN 0.051 NOT TAKEN 0.056 NOT TAKEN NOT TAKEN 0.050 0.054 NOT TAKEN NOT TAKEN 0.042 0.050 0.031 0.040 0.034 NOT TAKEN NOT TAKEN 0.042 0.057 4098 Covance 6329-212 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 15 ANIMAL NUMBER WEEK 36 WEEK 40 WEEK 44 WEEK 48 WEEK 52 WEEK 56 WEEK 60 WEEK 64 WEEK 68 WEEK 72 GROUP: FEMALE 9 - 1 PPM C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 0.073 0.053 0.057 0.053 0.062 0.057 0.053 0.057 0.062 0.064 0.074 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.064 0.048 0.054 0.053 0.059 0.051 0.048 0.056 SPILLED 0.060 0.067 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.064 0.045 0.053 0.051 0.056 0.048 0.044 0.058 0.054 0.049 0.073 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.059 0.047 0.053 0.046 0.054 0.046 0.045 0.055 0.054 0.058 0.063 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.059 0.042 0.047 0.046 0.052 0.048 0.042 0.055 0.048 0.060 0.051 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.042 0.049 0.042 0.049 SPILLED 0.045 0.054 0.046 0.053 0.053 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.047 0.045 0.043 0.043 0.045 0.037 0.048 0.048 0.023 0.043 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.040 0.036 0.045 0.050 0.042 0.001 0.050 0.047 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.070 0.048 0.046 0.039 0.046 0.052 NOT TAKEN 0.052 0.051 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.046 0.041 0.044 0.047 0.047 NOT TAKEN 0.052 0.051 NOT TAKEN 0.048 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 4099 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 9 - 1 PPM C92261 C92262 C92263 C92264 C92265 C92266 C92267 C92268 C92269 C92270 C92271 C92272 C92273 C92274 C92275 C92276 C92277 C92278 C92279 C92280 C92281 C92282 C92283 C92284 C92285 C92286 C92287 C92288 C92289 C92290 C92291 0.035 0.033 0.033 NOT TAKEN 0.027 0.036 NOT TAKEN 0.029 NOT TAKEN 0.037 0.035 0.034 NOT TAKEN NOT TAKEN 0.037 NOT TAKEN NOT TAKEN 0.033 0.032 NOT TAKEN 0.036 0.036 0.038 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.037 NOT TAKEN 0.038 0.034 0.034 0.030 NOT TAKEN NOT TAKEN 0.032 NOT TAKEN 0.024 NOT TAKEN 0.034 SPILLED 0.032 NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN 0.031 0.024 NOT TAKEN 0.033 0.035 0.033 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.033 NOT TAKEN 0.038 0.032 0.035 0.031 NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.022 NOT TAKEN 0.032 0.027 0.039 NOT TAKEN NOT TAKEN 0.037 NOT TAKEN NOT TAKEN 0.032 0.026 NOT TAKEN 0.030 0.034 0.032 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.037 0.032 0.038 0.030 NOT TAKEN NOT TAKEN 0.029 NOT TAKEN 0.024 NOT TAKEN 0.033 0.027 NOT TAKEN NOT TAKEN NOT TAKEN 0.037 NOT TAKEN NOT TAKEN 0.036 SPILLED NOT TAKEN 0.032 0.034 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.037 0.032 0.049 0.031 NOT TAKEN NOT TAKEN 0.028 NOT TAKEN 0.015 NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.035 NOT TAKEN NOT TAKEN 0.032 NOT TAKEN NOT TAKEN 0.030 0.031 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.030 NOT TAKEN 0.037 0.033 SPILLED 0.029 NOT TAKEN NOT TAKEN 0.028 NOT TAKEN 0.011 NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.034 NOT TAKEN NOT TAKEN 0.029 NOT TAKEN NOT TAKEN 0.030 0.033 0.027 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.029 NOT TAKEN NOT TAKEN 0.033 SPILLED 0.029 NOT TAKEN NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN 0.033 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.033 NOT TAKEN NOT TAKEN 0.034 NOT TAKEN NOT TAKEN 0.036 0.036 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.032 NOT TAKEN NOT TAKEN 0.037 NOT TAKEN 0.032 NOT TAKEN NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.035 NOT TAKEN NOT TAKEN 0.037 0.035 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.034 NOT TAKEN NOT TAKEN Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 16 4100 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 9 - 1 PPM C92292 C92293 C92294 C92295 C92296 C92297 C92298 C92299 C92300 C92301 C92302 C92303 C92304 C92305 C92306 C92307 C92308 C92309 C92310 C92311 C92312 C92313 C92314 C92315 C92316 C92317 C92318 C92319 C92320 C92321 C92322 NOT TAKEN NOT TAKEN 0.050 0.036 NOT TAKEN 0.041 NOT TAKEN 0.042 NOT TAKEN 0.035 0.034 NOT TAKEN 0.033 NOT TAKEN 0.034 0.042 0.052 0.038 NOT TAKEN 0.030 NOT TAKEN 0.037 0.037 NOT TAKEN 0.035 NOT TAKEN 0.035 0.034 NOT TAKEN 0.039 0.035 NOT TAKEN NOT TAKEN 0.039 0.031 NOT TAKEN 0.037 NOT TAKEN SPILLED NOT TAKEN 0.032 0.029 NOT TAKEN 0.034 NOT TAKEN 0.030 0.037 SPILLED 0.036 NOT TAKEN 0.032 NOT TAKEN 0.035 0.035 NOT TAKEN 0.030 NOT TAKEN 0.032 0.036 NOT TAKEN 0.040 0.029 NOT TAKEN NOT TAKEN 0.024 0.031 NOT TAKEN 0.036 NOT TAKEN 0.037 NOT TAKEN 0.029 0.027 NOT TAKEN 0.032 NOT TAKEN 0.032 0.034 SPILLED 0.034 NOT TAKEN 0.031 NOT TAKEN 0.035 0.034 NOT TAKEN SPILLED NOT TAKEN 0.032 0.033 NOT TAKEN 0.034 0.030 NOT TAKEN NOT TAKEN 0.018 0.030 NOT TAKEN 0.035 NOT TAKEN 0.037 NOT TAKEN NOT TAKEN 0.029 NOT TAKEN 0.029 NOT TAKEN 0.030 0.034 SPILLED 0.035 NOT TAKEN 0.029 NOT TAKEN 0.036 0.028 NOT TAKEN 0.032 NOT TAKEN 0.033 0.033 NOT TAKEN 0.036 0.031 NOT TAKEN NOT TAKEN 0.020 0.031 NOT TAKEN 0.032 NOT TAKEN 0.036 NOT TAKEN NOT TAKEN 0.017 NOT TAKEN 0.029 NOT TAKEN 0.029 0.034 0.047 0.033 NOT TAKEN 0.029 NOT TAKEN 0.034 SPILLED NOT TAKEN 0.027 NOT TAKEN 0.028 0.035 NOT TAKEN 0.033 0.032 NOT TAKEN NOT TAKEN 0.007 0.027 NOT TAKEN 0.032 NOT TAKEN 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.028 0.034 0.037 0.033 NOT TAKEN 0.030 NOT TAKEN 0.027 NOT TAKEN NOT TAKEN 0.020 NOT TAKEN 0.034 0.036 NOT TAKEN 0.033 0.029 NOT TAKEN NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.036 NOT TAKEN 0.031 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.030 0.038 0.038 0.035 NOT TAKEN 0.034 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.031 NOT TAKEN 0.034 0.041 NOT TAKEN 0.037 0.033 NOT TAKEN NOT TAKEN NOT TAKEN 0.032 NOT TAKEN 0.034 NOT TAKEN 0.022 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.026 0.031 NOT TAKEN 0.032 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.032 0.035 NOT TAKEN 0.035 0.035 Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 17 4101 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: MALE 9 - 1 PPM C92323 C92324 C92325 C92326 C92327 C92328 C92329 C92330 0.028 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN GROUP: FEMALE 9 - 1 PPM C92401 C92402 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 C92412 C92413 C92414 C92415 C92416 C92417 C92418 C92419 C92420 SPILLED NOT TAKEN NOT TAKEN 0.041 0.049 NOT TAKEN NOT TAKEN 0.047 0.048 0.048 NOT TAKEN 0.041 0.042 NOT TAKEN 0.040 0.049 0.045 NOT TAKEN 0.049 NOT TAKEN 0.028 NOT TAKEN NOT TAKEN 0.016 0.045 NOT TAKEN NOT TAKEN 0.043 SPILLED 0.042 NOT TAKEN 0.043 0.037 NOT TAKEN 0.043 0.045 0.039 NOT TAKEN 0.050 NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.019 NOT TAKEN NOT TAKEN NOT TAKEN 0.040 NOT TAKEN NOT TAKEN 0.042 0.046 0.043 NOT TAKEN 0.035 0.038 NOT TAKEN 0.043 0.042 0.013 NOT TAKEN 0.043 NOT TAKEN 0.028 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN 0.042 NOT TAKEN NOT TAKEN 0.045 0.044 0.043 NOT TAKEN 0.041 0.035 NOT TAKEN 0.030 0.041 0.009 NOT TAKEN 0.046 NOT TAKEN 0.029 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN 0.041 0.035 0.042 NOT TAKEN 0.040 0.026 NOT TAKEN NOT TAKEN 0.039 0.042 NOT TAKEN 0.043 NOT TAKEN 0.029 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.006 NOT TAKEN NOT TAKEN 0.048 0.006 0.040 NOT TAKEN 0.041 0.030 NOT TAKEN NOT TAKEN 0.031 NOT TAKEN NOT TAKEN 0.040 NOT TAKEN 0.030 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.040 NOT TAKEN 0.040 0.032 NOT TAKEN NOT TAKEN 0.044 NOT TAKEN NOT TAKEN 0.049 NOT TAKEN 0.024 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.037 NOT TAKEN 0.042 NOT TAKEN NOT TAKEN NOT TAKEN 0.049 NOT TAKEN NOT TAKEN 0.045 NOT TAKEN Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 18 4102 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 9 - 1 PPM C92421 C92422 C92423 C92424 C92425 C92426 C92427 C92428 C92429 C92430 C92431 C92432 C92433 C92434 C92435 C92436 C92437 C92438 C92439 C92440 C92441 C92442 C92443 C92444 C92445 C92446 C92447 C92448 C92449 C92450 C92451 0.046 NOT TAKEN 0.046 NOT TAKEN 0.050 0.056 NOT TAKEN NOT TAKEN NOT TAKEN 0.042 0.042 0.059 NOT TAKEN 0.057 NOT TAKEN 0.052 NOT TAKEN NOT TAKEN 0.043 0.046 NOT TAKEN NOT TAKEN 0.042 SPILLED 0.032 0.037 0.017 NOT TAKEN NOT TAKEN 0.037 0.057 0.041 NOT TAKEN 0.041 NOT TAKEN 0.042 0.055 NOT TAKEN NOT TAKEN NOT TAKEN 0.038 0.039 NOT TAKEN NOT TAKEN 0.051 NOT TAKEN 0.045 NOT TAKEN NOT TAKEN 0.043 NOT TAKEN NOT TAKEN NOT TAKEN 0.034 0.054 0.033 0.001 0.026 NOT TAKEN NOT TAKEN 0.017 SPILLED 0.041 NOT TAKEN 0.042 NOT TAKEN 0.041 0.054 NOT TAKEN NOT TAKEN NOT TAKEN 0.046 0.037 NOT TAKEN NOT TAKEN SPILLED NOT TAKEN 0.057 NOT TAKEN NOT TAKEN 0.040 NOT TAKEN NOT TAKEN NOT TAKEN 0.035 SPILLED 0.030 NOT TAKEN 0.029 NOT TAKEN NOT TAKEN 0.035 0.049 0.044 NOT TAKEN 0.044 NOT TAKEN 0.039 0.056 NOT TAKEN NOT TAKEN NOT TAKEN 0.040 0.038 NOT TAKEN NOT TAKEN 0.046 NOT TAKEN 0.053 NOT TAKEN NOT TAKEN 0.038 NOT TAKEN NOT TAKEN NOT TAKEN 0.032 0.044 0.028 NOT TAKEN 0.034 NOT TAKEN NOT TAKEN 0.035 0.047 0.036 NOT TAKEN 0.042 NOT TAKEN 0.034 0.058 NOT TAKEN NOT TAKEN NOT TAKEN 0.039 0.039 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.048 NOT TAKEN NOT TAKEN 0.037 NOT TAKEN NOT TAKEN NOT TAKEN 0.032 0.042 0.024 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.013 0.033 NOT TAKEN 0.041 NOT TAKEN 0.030 0.053 NOT TAKEN NOT TAKEN NOT TAKEN 0.040 0.036 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.036 NOT TAKEN NOT TAKEN NOT TAKEN 0.030 0.051 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.032 NOT TAKEN 0.047 NOT TAKEN NOT TAKEN 0.069 NOT TAKEN NOT TAKEN NOT TAKEN 0.039 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.041 NOT TAKEN NOT TAKEN NOT TAKEN 0.026 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.029 NOT TAKEN 0.047 NOT TAKEN NOT TAKEN 0.045 NOT TAKEN NOT TAKEN NOT TAKEN 0.042 0.039 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.041 NOT TAKEN NOT TAKEN NOT TAKEN 0.025 SPILLED NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 19 4103 Appendix 4 Individual Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER WEEK 76 WEEK 80 WEEK 84 WEEK 88 WEEK 92 WEEK 96 WEEK 100 WEEK 104 GROUP: FEMALE 9 - 1 PPM C92452 C92453 C92454 C92455 C92456 C92457 C92458 C92459 C92460 C92461 C92462 C92463 C92464 C92465 C92466 C92467 C92468 C92469 C92470 0.057 0.045 0.040 0.044 SPILLED 0.051 NOT TAKEN 0.056 0.047 NOT TAKEN 0.051 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED 0.037 0.034 0.042 0.038 0.029 NOT TAKEN 0.050 0.043 NOT TAKEN 0.047 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.046 0.034 NOT TAKEN 0.038 0.038 0.037 NOT TAKEN 0.046 0.027 NOT TAKEN 0.039 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.052 0.030 NOT TAKEN 0.039 0.035 NOT TAKEN NOT TAKEN 0.042 0.040 NOT TAKEN 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.047 0.021 NOT TAKEN 0.036 0.037 NOT TAKEN NOT TAKEN 0.032 0.023 NOT TAKEN 0.018 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN 0.055 0.020 NOT TAKEN 0.036 0.036 NOT TAKEN NOT TAKEN 0.032 SPILLED NOT TAKEN 0.014 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN 0.015 0.035 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN SPILLED NOT TAKEN NOT TAKEN NOT TAKEN 0.040 NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN Covance 6329-212 Covance 6329-228 3M T-6316.1 PAGE: 20 4104 APPENDIX 5 In d iv id u a l C lin ic al H e m a to lo g y D a ta In d iv id u a l C lin ic al C h e m istry D a ta In d iv id u a l C lin ic al U rin a ly sis D a ta In d iv id u a l U rin e C h e m istry D a ta Covance 6329-212 Covance 6329-228 3M T-6316.1 4105 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Dose Level: 0 9.14 9.40 8.68 8.44 8.31 7.37 8.34 6.49 8.45 8.58 16.1 16.1 15.3 15.4 15.8 13.9 14.6 12.5 16.2 15.6 8.32 .838 10 15.2 1.18 10 Dose Level: 1 8.72 8.33 7.75 8.57 8.33 8.09 7.98 8.31 9.21 8.55 15.8 14.6 14.4 14.7 15.1 14.5 14.1 15.6 15.9 15.5 8.38 .411 10 15.0 .64 10 Dosage Unit: ppm 46.2 46.1 44.6 46.0 45.9 40.7 43.6 36.8 47.4 45.8 50.5 49.1 51.4 54.5 55.2 55.2 52.3 56.7 56.2 53.4 44.3 3.23 10 53.4 2.55 10 Dosage Unit: ppm 46.1 43.1 42.4 44.0 44.2 42.6 41.6 45.0 46.2 45.5 52.8 51.8 54.7 51.3 53.1 52.6 52.2 54.2 50.1 53.2 44.1 1.62 10 52.6 1.35 10 17.6 17.1 17.6 18.3 19.0 18.9 17.5 19.3 19.2 18.1 18.3 .80 10 18.1 17.6 18.6 17.1 18.1 17.9 17.7 18.7 17.2 18.2 17.9 .53 10 34.8 34.8 34.2 33.6 34.4 34.2 33.4 34.1 34.3 34.0 34.2 .45 10 34.2 33.9 34.0 33.4 34.1 34.0 33.9 34.6 34.3 34.1 34.0 .31 10 1317 1003 1053 1019 1104 1019 1168 1128 1083 1042 1094 94. 10 1063 1217 1053 1104 1134 1078 1045 962 1018 1262 1094 90. 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4106 Covance 6329-212 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103///L MONO EOSIN X103///L X103///L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% Group: 8 Dose Level : 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 14.1 1.6 11.0 1.3 .2 .0 12 78 9 8.3 1.2 6.2 .7 .1 .0 15 75 9 10.0 1.0 7.6 1.0 .4 .0 10 76 10 10.7 1.4 8.4 .7 .2 .0 13 78 6 8.5 1.0 6.6 .6 .2 .0 12 78 7 8.4 1.3 5.8 1.1 .2 .0 15 69 13 12.3 1.9 9.3 .8 .3 .0 15 76 6 10.8 1.2 8.3 .8 .5 .0 11 77 8 12.9 1.1 10.9 .7 .2 .0 8 85 6 7.8 1.2 5.2 1.1 .3 .0 16 66 14 2 1 4 2 2 2 3 5 1 4 0 0 0 0 0 0 0 0 0 0 MEAN S.D. N 10.4 1.3 7.9 .9 .3 .0 13 76 93 0 2.18 .28 2.04 .23 .12 .00 2.6 5.2 2. 9 1.3 .0 10 10 10 10 10 10 10 10 10 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 9.3 1.7 6.5 .8 .2 .0 18 70 9 8.1 1.0 6.1 .9 .2 .0 12 75 10 11.3 1.1 9.2 .9 .2 .0 9 81 8 10.6 1.6 7.6 .9 .4 .0 16 72 9 9.0 .9 7.1 .9 .1 .0 10 78 10 11.4 .9 8.0 2.4 .1 .0 8 70 21 10.9 1.1 8.5 .9 .3 .1 10 78 9 6.6 1.1 4.5 .8 .1 .0 17 68 13 10.0 2.0 7.0 .8 .3 .0 19 69 8 14.4 1.6 10.7 1.4 .7 .0 11 74 10 3 2 1 3 1 1 2 2 3 5 0 0 0 0 0 0 0 0 0 0 MEAN S.D. N 10.2 1.3 7.5 1.1 .3 .0 13 74 11 2 0 2.12 .39 1.72 .50 .18 .03 4.1 4.4 3.9 1.3 .0 10 10 10 10 10 10 10 10 10 10 10 4107 Appendix 5 Individual Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 8 Dose Level: 0 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 - - Group: 9 Dose Level: 1 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 Covance 6329-228 3M T-6316.1 4108 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/m L Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Dose Level: 0 7.70 7.89 8.25 7.93 8.19 8.17 7.84 7.63 7.09 7.49 14.5 15.0 15.8 15.3 15.5 14.7 14.5 14.6 13.7 13.5 7.82 .358 10 14.7 .73 10 Dose Level: 1 7.61 8.12 7.61 8.64 8.56 7.79 7.99 7.93 7.89 7.95 15.2 15.2 15.0 16.1 15.6 14.7 15.2 15.2 15.1 14.9 8.01 .350 10 15.2 .39 10 Dosage Unit: ppm 42.4 43.8 46.0 44.4 45.5 43.3 42.7 43.2 39.1 40.5 55.1 55.5 55.8 56.0 55.6 53.0 54.4 56.6 55.2 54.1 43.1 2.10 10 55.1 1.05 10 Dosage Unit: ppm 44.3 44.8 42.7 47.4 45.6 43.0 44.1 42.7 44.1 43.8 58.2 55.1 56.1 54.8 53.3 55.2 55.2 53.9 55.9 55.1 44.2 1.44 10 55.3 1.32 10 18.9 19.1 19.1 19.3 19.0 17.9 18.4 19.2 19.3 18.0 18.8 .53 10 20.0 18.7 19.7 18.6 18.2 18.9 19.0 19.1 19.1 18.7 19.0 .53 10 34.3 34.3 34.3 34.5 34.1 33.9 33.9 33.9 35.0 33.3 34.2 .45 10 34.3 33.9 35.1 33.9 34.1 34.3 34.4 35.5 34.3 34.0 34.4 .52 10 946 1100 1022 1017 1007 1056 1123 990 1249 1130 1064 88 10 1022 1096 1176 1136 1122 1114 1267 958 1080 1144 1112 83 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4109 Covance 6329-212 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103///L X103//L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% Group: 8 Dose Level : 0 Dosage Unit: ppm C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 7.1 .6 5.7 .5 .3 .0 9 80 7 6.4 .7 4.9 .7 .2 .0 10 75 10 9.7 1.8 7.1 .6 .2 .0 18 73 6 4.8 .5 3.8 .4 .1 .0 10 78 8 8.0 .7 6.5 .6 .2 .0 8 82 7 5.1 .5 4.2 .3 .1 .0 10 82 6 12.0 1.0 10.0 .6 .4 .0 9 83 5 4.8 .9 3.5 .2 .1 .0 20 73 5 10.4 1.0 8.5 .6 .2 .0 10 82 6 5.0 .8 3.5 .5 .2 .0 16 70 9 4 3 2 3 2 3 3 3 2 5 0 0 0 0 0 0 0 0 0 0 MEAN S.D. N 7.3 .8 5.8 .5 .2 .0 12 78 7 3 0 2.61 .38 2.24 .16 .09 .00 4..3 4..7 1..7 .9 0 10 10 10 10 10 10 10 10 10 10 10 Group: 9 Dose Level : 1 Dosage Unit: ppm C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 5.1 .9 3.7 .4 .2 .0 17 72 8 3 0 7.3 1.0 5.8 .4 .2 .0 13 79 5 3 0 6.0 1.1 4.2 .5 .2 .0 18 71 8 3 0 8.9 .8 7.5 .4 .1 .0 9 84 5 1 0 7.4 .8 5.8 .6 .2 .0 11 77 8 3 0 6.5 1.1 5.0 .2 .1 .0 17 77 3 2 0 5.1 1.3 2.7 .8 .3 .0 25 53 16 5 0 6.7 .6 5.5 .4 .2 .0 8 83 6 3 0 7.0 1.0 5.5 .3 .2 .0 14 78 4 3 0 5.1 .8 3.9 .3 .1 .0 16 76 5 3 0 MEAN S.D. N 6.5 .9 5.0 .4 .2 .0 15 75 7 3 0 1.23 .20 1.37 .17 .06 .00 5..0 8..7 3..7 1..0 0 10 10 10 10 10 10 10 10 10 10 10 4110 Appendix 5 Individual Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 8 Dose Level: 0 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 - - Group: 9 Dose Level: 1 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 Covance 632 9-228 3M T-6 316.1 4111 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/m L Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Dose Level: 0 8.28 8.69 8.58 8.44 8.25 7.44 7.85 7.70 8.71 8.30 14.9 15.4 15.2 15.6 15.5 14.3 14.6 14.8 16.7 14.9 8.22 .430 10 15.2 .67 10 Dose Level: 1 9.00 7.70 8.01 8.61 8.51 8.03 7.54 8.21 8.93 8.21 16.0 14.0 14.9 14.5 15.1 14.3 13.5 15.3 15.8 14.9 8.28 .487 10 14.8 .78 10 Dosage Unit: ppm 44.2 45.4 44.1 45.5 45.1 41.5 43.4 43.2 49.1 44.8 53.4 52.3 51.4 53.9 54.7 55.8 55.2 56.1 56.3 54.0 44.6 1.98 10 54.3 1.63 10 Dosage Unit: ppm 46.8 41.0 43.9 43.6 45.4 42.6 40.2 45.1 46.6 43.9 52.0 53.2 54.8 50.6 53.4 53.1 53.4 54.9 52.2 53.5 43.9 2.19 10 53.1 1.28 10 17.9 17.7 17.7 18.5 18.8 19.2 18.6 19.2 19.2 18.0 18.5 .62 10 17.8 18.2 18.6 16.8 17.7 17.8 17.8 18.7 17.7 18.2 17.9 .54 10 33.6 33.9 34.4 34.3 34.4 34.3 33.7 34.1 34.1 33.4 34.0 .36 10 34.3 34.2 33.9 33.3 33.2 33.6 33.4 34.0 34.0 34.0 33.8 .39 10 1298 918 1131 948 1147 1070 1002 1129 1103 1081 1083 109 10 1027 1179 1137 1191 1091 1092 1004 992 1187 1218 1112 83 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4112 Covance 6329-212 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103//L X103//L BASO N-SEG% X103//L LYMPH% MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% Group: 8 Dose Level : 0 Dosage Unit: ppm C92191 11.3 2.0 7.6 1.2 .3 .0 18 68 11 3 0 C92193 6.5 1.1 5.2 .1 .1 .0 18 80 1 1 0 C92194 9.8 1.7 6.7 1.0 .4 .0 18 68 10 4 0 C92195 8.1 1.1 6.0 .8 .1 .0 14 74 10 2 1 C92197 7.4 1.4 4.9 .9 .2 .0 18 67 12 2 0 C92199 7.2 1.0 5.0 1.0 .1 .0 14 70 14 1 1 C92200 9.5 1.8 6.0 1.3 .4 .1 18 64 14 4 1 C92201 8.6 1.6 5.6 1.0 .3 .0 19 65 12 4 0 C92202 11.5 3.8 6.2 1.3 .2 .0 33 54 11 2 0 C92203 6.4 1.5 3.5 1.0 .4 .1 23 54 16 6 1 MEAN S.D. N 8.6 1.7 5.7 1.0 .2 .0 19 66 11 3 0 1.85 .81 1.12 .34 .13 .04 5..4 8..0 4..0 1..6 .5 10 10 10 10 10 10 10 10 10 10 10 Group: 9 Dose Level : 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 6.9 1.5 4.2 .9 .2 .0 22 61 14 8.2 1.6 5.2 1.2 .2 .0 19 64 15 8.4 1.1 6.2 .9 .1 .1 13 74 10 9.5 1.8 6.2 1.2 .3 .1 18 65 13 9.1 1.2 6.9 .9 .1 .0 13 76 10 10.6 1.4 5.6 3.1 .1 .4 13 53 30 8.7 1.2 6.3 .9 .2 .1 14 73 10 6.8 1.6 3.9 1.2 .1 .0 23 58 17 10.2 2.0 7.0 .9 .3 .0 19 69 9 10.3 1.4 7.4 1.2 .2 .0 13 72 12 3 2 2 4 1 1 2 1 3 2 0 0 1 1 0 3 1 1 0 0 MEAN S.D. N 8.9 1.5 5.9 1.2 .2 .1 17 66 14 2 1 1.34 .28 1.17 .67 .08 .13 4..0 7..6 6..2 1..0 .9 10 10 10 10 10 10 10 10 10 10 10 4113 Appendix 5 Individual Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 8 Dose Level: 0 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 - - Group: 9 Dose Level: 1 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 Covance 6329-228 3M T-6316.1 4114 Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/m L Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Dose Level: 0 7.54 7.46 7.71 7.31 7.36 7.50 7.23 6.89 7.22 6.89 14.5 14.9 14.7 14.2 14.1 13.8 13.0 13.6 14.1 12.5 7.31 .267 10 13.9 .75 10 Dose Level: 1 7.15 7.41 7.36 8.00 8.18 7.29 8.41 7.91 7.28 7.29 14.5 14.3 14.6 15.2 15.2 14.1 15.4 15.3 14.4 13.9 7.63 .451 10 14.7 .54 10 Dosage Unit: ppm 41.5 42.5 42.8 42.0 41.1 40.6 38.7 39.7 40.3 37.0 55.1 57.0 55.5 57.4 55.9 54.2 53.6 57.6 55.8 53.7 40.6 1.79 10 55.6 1.46 10 Dosage Unit: ppm 41.7 41.1 42.1 44.0 44.9 40.8 44.7 45.0 40.8 40.1 58.3 55.4 57.2 55.0 54.9 55.9 53.1 56.9 56.0 55.1 42.5 1.93 10 55.8 1.45 10 19.2 19.9 19.0 19.5 19.1 18.4 18.0 19.7 19.6 18.2 19.1 .66 10 20.2 19.4 19.9 19.0 18.6 19.3 18.3 19.3 19.8 19.1 19.3 .58 10 34.9 35.0 34.2 33.9 34.2 34.0 33.7 34.2 35.1 33.9 34.3 .50 10 34.6 34.9 34.7 34.5 33.9 34.5 34.4 34.0 35.3 34.7 34.6 .41 10 993 1054 1018 941 931 993 1201 967 1223 1061 1038 101 10 927 1019 1032 1061 1125 982 1472 963 1184 1210 1098 161 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4115 (9 8 . 4116 <N <N Ois <N u)Eh 1) O d) 2 U ro d fd > o uu HHOOOHOOOH oo\ w cCQ (N^CNCNCNHLnCNCNrO o\ o w OHOOOOOOOO r O C N C N i -- I C N C N 'FCN COC N P X O O ffftiEh 2 cd jj fd Q lx CD 0 i-- 1 [> 0 CN fd 4*5 B d) ffid) d) & ftT5d d) fd u -i-i ft d c -H i-- 1 U i-- 1 frd-j TJ CO d) 1-- 1 fd B d) ft H d M o\ i-- 1 w f t Eh CD C 5^ ft OP ft O ft c ft ftEfht HO Q M X2 C P Eh O CD f t Xp Eh CD M ft U m <; Eh ft U CD O OO ft HO UP ft P < ft U 05 X HD-i 05 CEh W X H H & off\tt 2 X P o\ CfDt CD P 3. cd o < H ft X p 3. M\ fw x P 3. o O i-- i 2X 05P3. ft \ 2 X J 1-1 X P CD :sW >- Wo J_ i-- I S x P CQ &o ' > C O C O I> C O ' > C O I> C O (N oooooooooo oooooooooo (N CN i-- l i -- l i -- l i -- I ' l l i-- l i -- I (N HHHHtN H C N H H H gfftt [>LDLD'>^^roro^'> Bfftt (NLn^LDftrocoLn^'X' d P 0) CD fd o i r i o i o o h r o c o o i n CO o t<roLD ro'>roi>(N LD ro U) ^ o i--I Fi--I d P d) CD fd CO o Q r o i > o * s ^ c o i - i o ( N * s i 1 *si1 ro ^ ^ L T )l> ro (N L r)ro ro (N U) O I--I st1I--I [>[>coLnco'X'fti>coLn OftcocoLD'>^coi>ft d) d) > d) p 'X'^roH^coi-lft^co 'X'^i>Lr)i>^(Nro'X'Lr) ^ ro o i--I > d) P SO (N ^ro^co^o^'x>'X'0 ^'>LD'>0^^')^LD oO P ft C ft 2 ft M2 sP <! 1 1 1 i i i fti--1 ro l> CO o i--1 (N ft ro ro ro ro ro ro ro d ro ro ro ro ro ro ro ro ro ro ftftftft ft ft0u (N (N (N (N (N (N (N (N (N (N ft ft ft ft CD U U U U U U U U U U < Q H 2 ft fti--1ro ID l> CO o i-- ft o o o O o o o o i--1i-- d ft ftft0 (N (N (N (N (N (N (N (N (N (N H f t f t f t f t f t f t f t CD U U U U U U U U U U s H 2 CD Appendix 5 Individual Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 8 Dose Level: 0 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 - - Group: 9 Dose Level: 1 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 Covance 6329-228 3M T-6316.1 4117 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106/^L G/DL % FL PG % X103/m L Group: 8 C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 MEAN S.D. N Group: 9 C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 MEAN S.D. N Dose Level: 0 8.74 7.46 8.55 7.98 7.57 9.11 7.88 8.43 7.89 8.40 16.2 14.3 15.7 13.9 14.4 15.7 15.2 15.4 14.8 16.0 8.20 .530 10 15.2 .78 10 Dose Level: 1 8.08 9.19 8.29 8.72 8.34 8.98 8.07 7.95 8.61 7.95 14.9 15.5 15.1 15.3 15.9 14.9 14.8 13.9 16.5 15.7 8.42 .439 10 15.2 .71 10 Dosage Unit: ppm 45.4 40.2 44.8 39.2 40.1 44.0 42.2 43.3 41.9 44.8 51.9 53.9 52.4 49.2 53.0 48.3 53.5 51.3 53.1 53.4 42.6 2.22 10 52.0 1.89 10 Dosage Unit: ppm 42.5 45.0 42.2 43.4 44.8 42.9 42.1 39.7 46.9 44.0 52.6 48.9 51.0 49.7 53.7 47.7 52.2 49.9 54.5 55.4 43.4 1.97 10 51.6 2.54 10 18.5 19.1 18.4 17.5 19.1 17.2 19.2 18.3 18.7 19.0 18.5 .69 10 18.5 16.9 18.2 17.5 19.0 16.5 18.4 17.5 19.1 19.8 18.1 1.04 10 35.6 35.5 35.2 35.5 36.0 35.6 35.9 35.6 35.3 35.6 35.6 .24 10 35.1 34.6 35.7 35.3 35.4 34.6 35.2 35.1 35.2 35.7 35.2 .38 10 928 1127 1110 1152 1459 1125 1292 1214 1324 978 1171 158 10 10 60 1310 1176 1207 1075 1278 899 1450 941 911 1131 185 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4118 Covance 6329-212 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103//L MONO EOSIN X103//L X103//L BASO N-SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% Group: 8 Dose Level : 0 Dosage Unit: ppm C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 5.2 2.1 2.6 .4 .1 .0 40 50 7 2 0 8.7 3.7 3.6 1.2 .1 .0 42 42 14 1 0 4.8 1.0 3.4 .3 .1 .0 22 71 6 1 0 6.4 2.1 2.8 1.3 .1 .0 34 44 21 2 1 8.9 2.0 5.8 .8 .2 .0 23 66 93 0 6.5 1.5 4.0 .9 .1 .1 24 60 14 1 1 6.9 1.5 4.1 1.1 .1 .0 22 59 16 2 0 5.7 2.3 2.8 .5 .2 .0 40 48 93 0 9.5 2.0 6.5 .7 .1 .0 21 69 8 1 0 5.8 1.5 3.6 .6 .2 .0 26 61 10 3 0 MEAN S.D. N 6.8 2.0 3.9 .8 .1 .0 29 57 11 2 0 1.64 .73 1.29 .34 .05 .03 8.6 10.4 4.7 .9 .4 10 10 10 10 10 10 10 10 10 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92267 5.5 1.2 3.4 .8 .1 .0 22 61 15 1 0 C92274 5.5 1.8 3.0 .6 .1 .0 32 55 11 2 0 C92286 7.1 2.1 3.8 1.1 .1 .0 29 54 15 2 0 C92287 5.2 1.7 3.0 .4 .1 .0 34 57 7 2 0 C92288 6.4 1.8 3.8 .7 .2 .0 27 59 11 2 0 C92303 11.4 5.1 4.8 1.4 .1 .0 45 42 12 1 0 C92315 5.1 1.1 3.3 .6 .1 .0 21 65 11 2 0 C92317 9.5 2.4 5.9 1.0 .2 .0 25 63 10 2 0 C92320 5.7 1.6 3.0 .9 .2 .0 28 53 15 3 0 C92324 5.5 1.1 3.5 .8 .1 .0 21 63 15 2 0 MEAN S.D. N 6.7 2.0 3.8 .8 .1 .0 28 57 12 2 0 2.12 1.17 .93 .29 .05 .00 7.3 6.7 2.7 .6 .0 10 10 10 10 10 10 10 10 10 10 10 4119 Appendix 5 Individual Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 8 Dose Level: 0 C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 - - Group: 9 Dose Level: 1 C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 Covance 6329-228 3M T-6316.1 4120 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL RBC HGB HCT MCV MCH MCHC PLT NUMBER X106///L G/DL FL PG X103///L Group: 8 C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 MEAN S.D. N Group: 9 C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 MEAN S.D. N Dose Level: 0 8.10 7.25 8.08 7.20 7.48 7.57 8.20 7.69 8.00 7.57 15.7 14.1 15.8 15.1 14.6 14.2 15.3 14.9 15.1 14.5 7.71 .362 10 14.9 .58 10 Dose Level: 1 8.50 7.55 7.97 7.58 8.45 7.23 7.95 7.63 7.29 7.66 15.5 14.7 15.0 14.7 15.5 14.3 15.5 14.4 14.6 14.7 7.78 .435 10 14.9 .46 10 Dosage Unit: ppm 43.3 38.6 43.4 41.9 40.0 39.4 43.6 41.4 42.3 40.5 53.5 53.2 53.7 58.2 53.5 52.1 53.2 53.8 52.9 53.5 41.4 1.77 10 53.8 1.63 10 Dosage Unit: ppm 43.0 41.1 41.4 40.7 43.7 40.0 42.8 41.1 41.3 41.0 50.6 54.4 52.0 53.6 51.7 55.2 53.9 53.8 56.6 53.5 41.6 1.16 10 53.5 1.75 10 19.4 19.4 19.6 21.0 19.6 18.8 18.6 19.3 18.9 19.1 19.4 .67 10 18.2 19.5 18.8 19.4 18.3 19.7 19.5 18.8 20.1 19.2 19.2 .61 10 36.3 36.5 36.5 36.1 36.6 36.0 35.1 35.9 35.8 35.7 36.0 .46 10 36.0 35.8 36.1 36.2 35.4 35.7 36.1 34.9 35.4 35.8 35.7 .41 10 709 1047 1097 915 929 855 870 1069 920 1003 941 117. 10 1065 1022 1276 1057 1104 1022 939 918 999 1130 1053 102. 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4121 Covance 6329-212 ANIMAL NUMBER WBC X103///L Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X103///L X103///L MONO EOSIN X103//L X103///L BASO N -SEG% X103///L LYMPH% MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% Group: 8 Dose Level: 0 Dosage Unit: ppm C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 6.6 1.6 4.4 .5 .1 .0 25 66 7 1 0 7.2 2.3 4.1 .7 .1 .0 32 57 10 1 0 5.9 1.9 3.1 .8 .1 .0 32 52 13 2 0 4.4 1.1 2.7 .5 .1 .0 25 62 11 2 0 6.1 1.5 3.8 .7 .2 .0 24 62 12 3 0 3.3 1.0 1.8 .4 .1 .0 29 56 12 3 0 3.2 .6 2.1 .4 .1 .0 20 65 14 2 0 3.6 1.2 1.9 .5 .1 .0 32 53 13 2 0 7.6 1.6 5.2 .6 .1 .0 21 69 8 1 0 4.5 1.4 2.5 .5 .1 .0 31 56 10 3 0 MEAN S.D. N 5.2 1.4 3.2 .6 .1 .0 27 60 11 2 0 1.64 .48 1.16 .13 .03 .00 4..7 5..8 2..3 .8 .0 10 10 10 10 10 10 10 10 10 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 6.4 2.6 2.9 .7 .1 .0 41 45 12 2 0 3.5 1.1 2.1 .2 .1 .0 32 61 5 2 0 5.6 1.3 3.3 .8 .1 .0 23 59 15 2 0 2.5 .6 1.2 .5 .1 .0 26 49 22 2 1 2.9 .6 1.8 .4 .1 .0 21 62 13 4 0 3.7 1.0 2.3 .3 .1 .0 27 62 92 0 5.2 .8 3.8 .4 .1 .0 16 73 92 0 9.3 1.1 7.1 1.0 .2 .1 12 76 10 2 1 7.7 2.6 4.2 .6 .2 .0 34 55 8 3 0 4.4 1.0 2.8 .6 .1 .0 23 63 12 2 0 MEAN S.D. N 5.1 1.3 3.2 .6 .1 .0 26 60 12 2 0 2.18 .73 1.66 .24 .04 .03 8..6 9..5 4..6 .7 .4 10 10 10 10 10 10 10 10 10 10 10 4122 Appendix 5 Individual Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER ANISO POLY POIK HYPO TOXNEUT Group: 8 Dose Level: 0 C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 - - Group: 9 Dose Level: 1 C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 - - Dosage Unit: ppm ----------- Dosage Unit: ppm ----------- Covance 6329-212 Covance 6329-228 3M T-6316.1 4123 Appendix 5 Individual Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 8 C92192 C92196 C92207 C92216 C92259 MEAN S.D. N Group: 9 C92264 C92280 C92284 C92300 C92329 MEAN S.D. N Dose Level 0 11 8 8 4 6 7 2.6 5 Dose Level 1 6 2 4 10 11 7 3.8 5 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 Covance 6329-228 3M T-6316.1 4124 Appendix 5 Individual Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 8 C92332 C92352 C92355 C92366 C92373 MEAN S.D. N Group: 9 C92402 C92435 C92463 C92467 C92470 MEAN S.D. N Dose Level: 0 2 1 4 3 3 3 1.1 5 Dose Level: 1 5 0 5 6 4 4 2.3 5 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 Covance 6329-228 3M T-6316.1 4125 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 8 Dose Level: 0 Dosage Unit: ppm C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 107 13 .7 7 .3 4.9 2.4 .1 48 108 11 .8 7 .4 5.2 2.2 .0 52 126 14 .7 6 .6 4.6 2.0 .0 57 108 11 .6 6 .2 4.7 1.5 .0 59 109 14 .6 6 .8 4.6 2.2 .0 66 111 16 .5 6 .4 4.5 1.9 .1 56 130 18 .7 7 .3 5.0 2.3 .1 64 124 14 .6 7 .0 4.6 2.4 .0 64 102 13 .8 7 .1 4.8 2.3 .0 69 108 13 .6 6 .8 4.7 2.1 .0 51 MEAN S.D. N 113 14 .7 6 .9 4.8 2.1 .0 59 9.6 2. 1 .10 .40 .22 .28 .05 7.0 10 10 10 10 10 10 10 10 Group: 9 Dose Level: 1 Dosage Unit: ppm C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 109 12 .6 7 .0 4.7 2.3 .0 48 118 14 .6 7 .1 4.7 2.4 .1 56 109 18 .5 6 .8 4.7 2.1 .1 48 110 14 .7 7 .4 4.6 2.8 .0 77 113 12 .7 6 .9 4.8 2.1 .1 56 100 15 .7 6 .7 4.3 2.4 .0 50 121 11 .7 7 .1 4.9 2.2 .0 78 126 12 .4 7 .4 4.9 2.5 .1 70 115 13 .5 6 .4 4.5 1.9 .0 75 119 11 .6 7 .3 4.8 2.5 .1 75 MEAN S.D. N 114 13 .6 7 .0 4.7 2.3 .0 63 7.4 2. 1 .11 .32 .19 .26 .05 12.8 10 10 10 10 10 10 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4126 Covance 6329-212 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Dose Level: 0 101 34 162 38 99 32 103 38 126 36 85 40 105 58 115 32 95 32 105 46 110 21.4 10 39 8.1 10 Dose Level: 1 136 57 131 101 90 31 99 30 113 36 112 39 118 39 94 61 109 42 105 43 111 14.9 10 48 21.2 10 Dosage Unit: ppm 2 10.6 7 10.4 2 10.4 1 10.5 4 10.5 3 10.8 0 10.8 1 10.2 1 10.7 0 10.6 2 2.1 10 10.6 .19 10 Dosage Unit: ppm 5 10.4 2 10.6 3 10.5 7 10.8 0 10.7 2 10.3 1 10.8 0 10.4 3 10.2 3 10.9 3 2.2 10 10.6 .24 10 7.1 7.2 7.6 6.3 7.2 8.3 8.2 6.6 6.9 7.2 7.3 .63 10 6.6 7.3 7.2 7.1 7.4 6.8 6.9 6.7 6.7 7.4 7.0 .31 10 147 148 147 146 147 145 146 147 147 146 147 .8 10 6.0 5.5 6.4 5.5 5.9 5.5 6.6 5.8 6.2 5.8 5.9 .39 10 146 146 146 147 146 147 146 148 147 148 147 .8 10 6.0 6.3 6.3 6.4 6.8 6.3 6.0 5.7 6.2 6.6 6.3 .31 10 106 108 107 107 107 104 102 108 106 107 106 1 10 107 108 107 107 106 108 107 107 108 107 107 .6 10 Covance 6329-228 3M T-6316.1 4127 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Dose Level: 0 126 17 105 14 118 16 120 14 113 14 123 18 105 11 118 12 117 14 99 12 114 8.7 10 14 2. 3 10 Dose Level: 1 114 11 114 13 110 18 104 17 111 18 104 14 107 16 112 13 110 17 113 14 110 3.8 10 15 2. 4 10 Dosage Unit: ppm .8 7 .5 .9 7 .8 .9 8 .5 1.0 8 .6 .8 7 .3 .7 8 .1 .7 8 .0 .8 8 .8 .7 8 .5 .5 9 .2 .8 8 .2 .14 .60 10 10 Dosage Unit: ppm .8 7 .4 .8 8 .1 .6 9 .4 .7 8 .1 .7 8 .1 .8 8 .6 .8 7 .4 .9 7 .2 .5 7 .8 .8 7 .3 .7 7 .9 .12 .68 10 10 5.7 5.8 6.1 6.1 5.3 6.0 6.0 6.6 6.6 7.1 6.1 .52 10 5.6 6.5 7.1 5.6 5.8 6.3 5.3 5.0 5.9 5.1 5.8 .66 10 1.8 2.0 2.4 2.5 2.0 2.1 2.0 2.2 1.9 2.1 2.1 .22 10 1.8 1.6 2.3 2.5 2.3 2.3 2.1 2.2 1.9 2.2 2.1 .27 10 .1 64 .0 80 .2 96 .3 109 .0 79 .1 93 .0 78 .3 88 .5 115 .1 140 .2 94 .16 22.1 10 10 .3 68 .2 90 .3 138 .1 87 .0 97 .0 84 .0 94 .1 76 .3 65 .0 86 .1 88 .13 20.3 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4128 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Dose Level: 0 83 42 88 35 91 34 98 30 94 37 85 27 108 37 80 35 275 112 98 55 110 58.6 10 44 24.9 10 Dose Level: 1 104 34 103 36 99 43 113 48 157 83 82 29 140 74 83 27 104 36 90 34 108 24.1 10 44 19.1 10 Dosage Unit: ppm 2 10.9 2 11.3 2 11.9 1 11.6 4 10.6 3 11.1 4 11.3 3 11.4 0 11.7 1 12.5 2 1.3 10 11.4 .54 10 Dosage Unit: ppm 2 11.0 4 11.3 0 11.4 1 11.3 0 10.9 1 11.2 6 11.7 1 10.7 0 11.3 0 10.9 2 2.0 10 11.2 .29 10 6.1 6.4 6.5 5.8 5.5 5.4 6.7 5.6 6.2 5.9 6.0 .44 10 6.3 5.9 6.6 7.2 6.2 5.9 6.8 6.1 6.6 6.8 6.4 .43 10 146 145 149 147 144 144 144 145 145 144 145 1.6 10 5.3 6.0 6.6 6.1 6.0 5.6 5.8 5.5 5.5 5.8 5.8 .38 10 147 146 146 148 146 146 148 146 147 147 147 .8 10 5.2 5.6 5.7 6.3 5.8 6.1 6.2 6.3 6.2 6.1 6.0 .36 10 108 106 106 108 106 107 104 106 103 103 106 1.8 10 106 105 103 107 107 106 109 111 107 108 107 2.2 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4129 Appendix 5 Individual Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 8 C92255 C92256 C92257 C92258 C92260 MEAN S.D. N Group: 9 C92325 C92326 C92327 C92328 C92330 MEAN S.D. N Dose Level: 0 7 5 4 5 7 6 1.3 5 Dose Level: 1 5 4 5 7 6 5 1.1 5 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 Covance 6329-228 3M T-6316.1 4130 Appendix 5 Individual Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER PCOAO IU/G Group: 8 C92396 C92397 C92398 C92399 C92400 MEAN S.D. N Group: 9 C92464 C92465 C92466 C92468 C92469 MEAN S.D. N Dose Level: 0 4 3 4 4 2 3 .9 5 Dose Level: 1 4 6 7 3 4 5 1.6 5 Dosage Unit: ppm Dosage Unit: ppm Covance 6329-212 Covance 6329-228 3M T-6316.1 4131 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Dose Level : 0 85 12 101 13 114 11 93 12 104 11 112 12 117 14 94 13 102 11 106 10 103 10.1 10 12 1.2 10 Dose Level: 1 112 13 107 13 102 13 103 14 107 12 105 14 121 11 120 13 119 13 116 12 111 7.3 10 13 .9 10 Dosage Unit: ppm .7 7.1 .6 7.1 .6 6.6 .6 6.3 .6 7.0 .5 6.6 .7 7.0 .6 7.2 .7 7.2 .6 6.6 .6 6.9 .06 .32 10 10 Dosage Unit: ppm .6 7.1 .6 6.9 .6 7.1 .6 7.4 .7 7.2 .6 6.5 .6 7.1 .7 7.6 .6 7.0 .6 7.2 .6 7.1 .04 .29 10 10 4.7 5.0 4.5 4.7 4.6 4.5 4.6 4.6 4.8 4.4 4.6 .17 10 4.8 4.5 4.7 4.4 4.8 4.3 4.7 4.8 4.7 4.8 4.6 .18 10 2.4 2.1 2.1 1.6 2.4 2.1 2.4 2.6 2.4 2.2 2.2 .28 10 2.3 2.4 2.4 3.0 2.4 2.2 2.4 2.8 2.3 2.4 2.5 .25 10 .2 51 .1 74 .2 74 .1 61 .1 80 .1 78 .2 96 .1 76 .1 87 .1 72 .1 75 .05 12.5 10 10 .2 62 .2 78 .2 73 .1 114 .2 74 .1 65 .1 104 .2 90 .1 98 .2 82 .2 84 .05 17 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4132 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Dose Level: 0 127 40 120 33 156 40 103 36 124 33 84 30 138 109 139 37 95 36 116 39 120 21.8 10 43 23.3 10 Dose Level: 1 113 53 127 80 90 24 109 30 119 39 83 30 113 38 91 68 153 50 105 34 110 20.4 10 45 18.1 10 Dosage Unit: ppm 2 10.5 0 10.5 2 10.3 2 10.6 3 10.8 2 10.7 3 10.6 2 10.5 2 10.8 2 10.4 2 .8 10 10.6 .16 10 Dosage Unit: ppm 1 10.7 1 10.8 2 10.9 1 10.7 1 10.4 2 10.6 0 10.8 1 10.8 3 11.1 2 11.0 1 .8 10 10.8 .20 10 6.9 7.1 7.3 6.3 7.2 7.8 6.6 6.8 7.1 7.0 7.0 .41 10 7.4 7.5 6.8 6.7 6.3 6.5 6.7 7.4 7.6 7.3 7.0 .47 10 148 147 145 145 147 146 145 147 148 147 146 1.2 10 5.5 5.9 5.9 5.3 6.4 5.7 6.2 5.9 6.3 6.0 5.9 .34 10 148 148 148 147 145 146 146 148 147 148 147 1.1 10 6.5 6.2 6.2 6.5 6.1 6.3 5.9 5.8 6.5 6.7 6.3 .29 10 103 108 106 104 106 105 102 102 104 106 105 2 10 105 106 106 103 101 109 104 103 109 104 105 2 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4133 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Dose Level: 0 110 16 110 14 106 16 108 13 111 12 107 16 107 12 113 12 101 13 85 13 106 8.0 10 14 1. 7 10 Dose Level: 1 108 11 110 12 101 12 92 13 102 16 97 10 110 15 114 14 106 11 96 16 104 7.2 10 13 2. 2 10 Dosage Unit: ppm .7 8.6 .7 7.5 .7 7.9 .7 8.7 .7 8.2 .7 8.4 .7 8.0 .7 8.3 .7 8.6 .6 8.8 .7 8.3 .03 .41 10 10 Dosage Unit: ppm .7 7.4 .7 8.4 .7 8.7 .8 8.5 .8 8.5 .7 8.5 .8 8.1 .7 7.5 .7 8.6 .7 8.1 .7 8.2 .05 .45 10 10 6.5 5.7 5.8 6.3 6.3 6.3 5.6 6.3 6.2 6.4 6.1 .32 10 5.8 6.7 6.7 6.1 5.8 6.1 5.0 5.3 6.7 5.2 5.9 .64 10 2.1 1.8 2.1 2.4 1.9 2.1 2.4 2.0 2.4 2.4 2.2 .23 10 1.6 1.7 2.0 2.4 2.7 2.4 3.1 2.2 1.9 2.9 2.3 .50 10 .2 112 .2 81 .2 76 .2 144 .2 110 .2 117 .2 117 .2 103 .2 142 .2 164 .2 117 .00 27 10 10 .2 64 .2 105 .2 135 .2 119 .2 110 .1 90 .3 186 .1 79 .2 97 .1 122 .2 111 .06 33 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4134 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Dose Level: 0 125 91 68 31 83 31 101 46 78 39 93 30 229 149 91 35 103 46 175 86 115 50.2 10 58 38. 8 10 Dose Level: 1 97 47 109 46 101 36 283 155 244 164 74 27 612 384 103 30 71 32 89 34 178 168.9 10 96 113. 8 10 Dosage Unit: ppm 1 11.3 3 11.1 2 11.1 2 11.8 0 11.0 2 11.2 7 11.1 1 11.0 2 11.0 1 12.1 2 1.9 10 11.3 .38 10 Dosage Unit: ppm 2 11.0 1 11.1 2 11.4 3 11.1 3 10.9 2 11.1 22 12.1 2 10.6 2 11.7 2 11.4 4 6.3 10 11.2 .43 10 5.7 6.0 4.6 6.2 5.0 5.4 6.2 5.0 6.0 6.2 5.6 .59 10 6.2 5.2 5.4 5.6 6.8 5.7 6.8 6.1 6.0 6.8 6.1 .59 10 146 146 142 144 144 144 143 145 145 144 144 1. 3 10 4.5 6.2 5.5 5.1 5.1 5.5 5.4 4.9 4.6 5.4 5.2 .50 10 146 145 147 145 144 144 148 147 145 145 146 1. 3 10 5.4 5.3 5.6 4.7 4.9 5.6 6.7 5.8 5.3 5.5 5.5 .54 10 101 106 103 105 106 104 102 102 97 102 103 2 10 106 102 104 104 100 102 108 105 103 103 104 2 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4135 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 8 C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 MEAN S.D. N Group: 9 C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 MEAN S.D. N Dose Level: 0 94 10 105 9 105 12 106 11 148 12 140 13 129 11 106 16 145 14 116 13 119 19.5 10 12 2. 0 10 Dose Level: 1 107 13 152 12 117 8 126 12 130 13 114 15 137 14 135 11 118 8 131 12 127 13.2 10 12 2. 3 10 Dosage Unit: ppm .7 7.1 .6 6.9 .8 7.4 .6 6.6 .6 7.2 .7 7.6 .6 6.6 .8 7.5 .8 7.1 .7 6.9 .7 7.1 .09 .35 10 10 Dosage Unit: ppm .7 6.9 .7 7.6 .6 6.8 .7 7.6 .7 7.3 .7 6.8 .7 6.8 .7 7.1 .7 7.2 .7 7.3 .7 7.1 .03 .31 10 10 5.1 4.4 4.7 3.9 4.6 4.7 4.3 5.1 4.4 4.8 4.6 .37 10 4.7 4.9 4.5 4.9 4.8 3.9 4.7 4.7 4.6 4.3 4.6 .31 10 2.0 2.5 2.7 2.7 2.6 2.9 2.3 2.4 2.7 2.1 2.5 .29 10 2.2 2.7 2.3 2.7 2.5 2.9 2.1 2.4 2.6 3.0 2.5 .30 10 .1 93 .1 96 .2 134 .1 78 .2 139 .2 80 .1 90 .2 80 .2 179 .2 85 .2 105 .05 33 10 10 .1 82 .1 131 .1 74 .2 108 .2 101 .1 38 .2 98 .2 104 .2 88 .1 200 .2 102 .05 42 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4136 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 8 C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 MEAN S.D. N Group: 9 C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 MEAN S.D. N Dose Level: 0 118 33 66 28 151 65 98 29 83 41 247 414 61 38 86 43 77 36 93 44 108 55.4 10 77 118. 8 10 Dose Level: 1 103 36 144 64 80 42 158 37 185 55 101 34 91 32 71 34 108 56 61 27 110 40.0 10 42 12. 3 10 Dosage Unit: ppm 0 10.7 2 9.3 1 10.6 2 10.1 1 10.4 3 11.1 1 9.9 3 9.7 2 10.8 0 10.3 2 1.1 10 10.3 .55 10 Dosage Unit: ppm 1 10.1 4 11.0 1 10.4 1 10.5 3 10.6 2 9.9 1 10.4 0 10.9 0 10.8 1 10.6 1 1.3 10 10.5 .34 10 5.8 5.5 8.1 5.6 5.4 6.4 6.6 7.1 6.2 5.3 6.2 .89 10 6.6 7.0 5.7 6.9 7.2 6.2 6.0 6.3 6.0 5.8 6.4 .53 10 147 147 147 148 145 150 149 158 149 149 149 3. 5 10 5.2 5.6 6.2 6.5 5.6 6.5 5.9 7.2 6.1 6.5 6.1 .58 10 158 149 150 156 149 154 147 148 144 149 150 4. 3 10 6.6 6.4 6.4 7.0 6.8 6.2 6.1 6.9 5.5 5.7 6.4 .50 10 105 104 103 107 104 108 106 113 104 108 106 3 10 114 105 106 110 108 110 105 106 100 106 107 3 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4137 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Group: 8 C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 MEAN S.D. N Group: 9 C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 MEAN S.D. N Dose Level: 0 119 13 92 11 143 10 119 11 113 12 100 10 131 11 120 11 105 12 86 13 113 17.5 10 11 1. 1 10 Dose Level: 1 117 17 95 14 98 13 111 10 115 13 122 15 108 15 103 13 110 10 99 17 108 8.9 10 14 2. 5 10 Dosage Unit: ppm .8 8.2 .8 9.4 .7 8.0 .8 8.1 .8 8.7 .6 8.5 .8 8.4 .8 8.0 .7 8.0 .8 8.2 .8 8.4 .07 .44 10 10 Dosage Unit: ppm .9 9.0 .9 8.9 .8 8.9 .8 8.3 .7 8.2 .8 7.7 .9 9.0 .8 8.6 .7 7.9 .8 8.6 .8 8.5 .07 .47 10 10 6.1 6.4 5.9 5.9 6.0 5.9 6.4 5.6 5.2 5.8 5.9 .36 10 5.8 6.1 5.4 6.1 5.9 5.4 6.4 5.8 5.1 6.1 5.8 .40 10 2.1 3.0 2.1 2.2 2.7 2.6 2.0 2.4 2.8 2.4 2.4 .34 10 3.2 2.8 3.5 2.2 2.3 2.3 2.6 2.8 2.8 2.5 2.7 .41 10 .7 157 .2 152 .7 214 .2 160 .2 128 .2 156 .2 104 .1 114 .2 98 .2 142 .3 142 .22 34 10 10 .2 128 .2 112 .2 128 .2 105 .2 129 .1 105 .2 146 .3 133 .2 171 .1 108 .2 126 .06 20 10 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4138 ANIMAL NUMBER Appendix 5 Individual Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Group: 8 C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 MEAN S.D. N Group: 9 C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 MEAN S.D. N Dose Level: 0 119 83 95 50 77 36 80 53 85 36 86 30 70 46 119 77 168 101 97 29 100 29.1 10 54 24. 7 10 Dose Level: 1 213 130 100 47 88 39 76 32 102 40 77 30 160 120 593 213 67 25 84 43 156 160.1 10 72 62. 1 10 Dosage Unit: ppm 1 10.4 3 11.6 1 11.2 2 10.9 2 11.7 2 11.5 1 10.9 1 10.6 3 11.4 0 10.9 2 1.0 10 11.1 .44 10 Dosage Unit: ppm 2 11.2 0 10.3 1 11.0 0 11.2 2 11.6 0 10.3 2 11.3 9 11.7 2 10.5 2 10.9 2 2.6 10 11.0 .50 10 5.8 5.6 5.9 4.4 6.0 5.9 5.2 4.0 7.2 3.8 5.4 1.05 10 6.2 4.6 6.7 6.3 5.6 5.1 4.5 8.0 4.4 5.3 5.7 1.14 10 157 143 150 146 144 143 148 148 148 147 147 4. 1 10 5.1 5.2 5.5 5.4 5.4 5.3 5.8 5.9 6.0 4.8 5.4 .37 10 145 143 150 148 147 146 149 149 146 150 147 2. 3 10 4.7 4.2 6.3 5.4 6.3 5.5 5.4 5.5 4.6 5.5 5.3 .68 10 104 100 97 104 102 101 105 106 102 103 102 2 10 100 97 105 105 105 103 104 104 104 108 104 3 10 Covance 6329-212 Covance 6329-228 3M T-6316.1 4139 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Dose Level: 0 10.3 31.0 5.6 8.3 32.9 42.4 48.5 a 13.7 24.0 10.6 1.021 1.006 1.038 1.027 1.007 1.004 1.003 1.020 1.008 1.020 22.7 15.31 10 1.015 .0116 10 Dose Level: 1 16.2 47.8 a 23.0 12.4 20.3 5.5 17.4 27.1 9.5 39.4 1.015 1.005 1.014 1.019 1.014 1.036 1.019 1.012 1.023 1.010 21.9 13.22 10 1.017 .0085 10 Dosage 7.0 7.5 6.5 7.0 7.0 7.5 7.0 7.0 7.0 7.5 7.1 .32 10 Dosage 7.0 7.0 7.0 7.5 7.5 7.0 7.0 7.0 7.5 7.0 7.2 .24 10 ppm ppm a Sample was overflowing at the time of collection. 4140 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 Dose Level: 0 30 10 30 30 10 030 30 10 30 - Dose Level: 1 10 10 10 30 10 30 30 10 30 10 - Dosage Unit: ppm ----+ --+ ------- - +++ - - +++ --+-- Dosage Unit: ppm --------+ --+---------- - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4141 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 Dose Level: 0 00 00 00 00 00 01 00 10 11 00 Dose Level: 1 00 00 00 00 00 00 00 00 00 00 Dosage Unit:: ppm 110 12 0 110 02 0 010 12 0 110 12 0 110 12 0 Dosage Unit: ppm 12 0 12 0 02 0 12 0 12 0 12 0 12 0 12 0 02 0 110 4D BJ PQ 0 AJ Q 4D BJ PQ 4D BJ PQ 0 AJ Q 0 AJ P 0 AJ Q 4D BJ PQ 0 AJ Q 4D BJ PQ 4D BJ PQ 0 AJ Q 0 BJ Q 4D BJ PQ 4D BJ PQ 4D BJ PQ 4D BJ PQ 0 BJ Q 4D BK Q 0 AJ Q Covance 6329-212 Covance 6329-228 3M T-6316.1 4142 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Dose Level: 0 9.0 50.5a 4.2 5.1 10.3 5.0 16.2 11.9 5.3 7.3 1.013 1.001 1.024 1.020 1.016 1.024 1.015 1.011 1.028 1.014 12.5 13.88 10 1.017 .0078 10 Dose Level: 1 22.3 40.8 4.6 49.8 a 5.6 2.0 17.8 18.5 50.7 a 7.5 1.007 1.001 1.026 1.002 1.006 1.034 1.009 1.010 1.002 1.020 22.0 18.74 10 1.012 .0112 10 Dosa 7.0 6.5 6.5 7.0 6.5 6.5 7.0 7.0 6.5 7.0 6.8 .2 10 Dosa 7.0 6.5 6.5 7.0 7.0 6.5 7.0 6.5 6.5 6.5 6.7 .2 10 ppm ppm a Sample was overflowing at the time of collection. 4143 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 Dose Level: 0 10 010 10 10 10 00100 30 - Dose Level: 1 0030 0030 00010 - Dosage Unit: ppm --------------------+ Dosage Unit: ppm ----- - +++ --------------- - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4144 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 Dose Level: 0 00 00 00 00 00 00 00 00 00 00 Dose Level: 1 00 00 11 00 00 00 00 01 00 00 Dosage Unit: ppm 02 0 010 12 0 130 110 12 0 110 110 110 02 0 Dosage Unit: ppm 12 0 010 12 0 010 110 12 0 110 12 0 010 12 0 4D 0 4D 3B4D 1D 4D 4D 4D 1D 4D 0 0 3D 0 0 4D 0 1B2D 0 0 BJ AJ BJ BJ BJ BJ BJ BJ BJ BJ AJ AJ BJ AJ AJ BJ AJ BJ AJ BJ P P P P P P P - P P P P PS Covance 6329-212 Covance 6329-228 3M T-6316.1 4145 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Dose Level: 0 10.0 8.3 8.0 5.5 44.5 17.5 9.5 5.8 7.2 22.0 1.020 1.024 1.032 1.033 1.006 1.014 1.020 1.027 1.026 1.020 13.8 12.00 10 1.022 .0081 10 Dose Level: 1 9.0 7.8 12.3 48.0 a 8.0 1.6 11.8 23.0 9.3 23.2 1.027 1.031 1.019 1.008 1.032 1.053 1.031 1.017 1.023 1.015 15.4 13.27 10 1.026 .0125 10 Dosa 7.0 7.5 6.5 7.0 7.0 7.5 6.5 6.5 6.5 7.0 6.9 .3 10 Dosa 7.5 6.5 7.0 7.0 7.0 7.0 7.0 7.0 7.5 7.0 7.0 .2 10 ppm ppm a Sample was overflowing at the time of collection. 4146 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 Dose Level: 0 10 100 100 10 010 100 30 100 100 - Dose Level: 1 30 30 30 100 10 100 100 10 10 30 - Dosage Unit: ppm -- ++ -------+- - + - Dosage Unit: ppm +-+-------+---+-+---+ - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4147 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 Group: 9 C92261 C92262 C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 Dose Level: 0 00 00 00 00 00 00 00 00 01 00 Dose Level: 1 00 00 01 00 00 10 00 00 00 10 Dosage Unit:: ppm 110 010 010 130 110 110 12 0 12 0 12 0 130 Dosage Unit: ppm 02 0 110 02 0 12 0 110 110 110 12 0 010 010 4D 3D 1B 4D 0 3D 1D 0 1B1D 4D 4D 3D 1D 4D 4D 3D 4D 4D 3D 4D BJ BJ BJ BK AJ BJ BJ BJ BJ BJ BJ BJ BJ AJ BJ BJ BJ BJ BJ BJ PQ Q PQ Q Q PQ PQ Q Q Q PQ Q PQ Q PQ PQ PQ PQ PQ Covance 6329-212 Covance 6329-228 3M T-6316.1 4148 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Dose Level: 0 7.0 2.6 2.3 47.5 a 8.3 2.6 27.0 6.8 47.0 a 20.0 1.020 1.027 1.040 1.003 1.016 1.041 1.011 1.013 1.006 1.010 17.1 17.79 10 1.019 .0134 10 Dose Level: 1 13.0 14.2 41.8 9.8 .5 46.0 43.5 5.0 10.0 15.8 1.020 1.019 1.006 1.014 1.047 1.005 1.006 1.026 1.020 1.012 20.0 17.04 10 1.018 .0126 10 Dosage 6.5 6.5 7.0 6.5 6.5 6.5 6.5 7.0 6.5 6.5 6.6 .21 10 Dosage 6.5 6.5 6.5 6.5 6.5 6.5 6.5 7.0 6.5 6.5 6.6 .16 10 ppm ppm a Sample was overflowing at the time of collection. 4149 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 8 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 Group: 9 C92401 C92403 C92404 C92405 C92406 C92407 C92408 C92409 C92410 C92411 Dose Level: 0 100 10 10 0010 00100 30 Dose Level: 1 010 010 10 0010 00- Dosage Unit: ppm ------------------- Dosage Unit: ppm ------+ --------------- - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4150 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 8 Dose Level: 0 C92331 C92333 C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 Group: 9 Dose Level: 1 C92401 C92403 C92404 C92405 C92406 QS C92407 C92408 C92409 C92410 C92411 0 0 0 0 - 0 0 0 0 0 0 0 0 0 - 0 0 0 1 0 Dosage Unit: ppm 110 110 12 0 110 110 110 110 110 110 12 0 Dosage Unit: ppm 110 110 030 110 --12 0 110 110 110 12 0 0 BJ P 0 BJ P 4D BJ PQ 0 AJ 1D BJ P 3D BJ P 0 BJ 0 BJ 0 AJ 2B BJ - 0 BJ 0 BJ 2B BJ 0 BJ P - BJ 0 AJ 0 AJ 4D BJ P 0 BJ 0 BJ - Covance 6329-212 Covance 6329-228 3M T-6316.1 4151 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 8 C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 MEAN S.D. N Group: 9 C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 MEAN S.D. N Dose Level: 0 15.2 9.8 18.0 49.5 a 7.0 10.3 14.2 6.3 9.2 7.3 1.014 1.021 1.025 1.004 1.034 1.030 1.024 1.041 1.037 1.027 14.7 12.83 10 1.026 .0110 10 Dose Level: 1 3.3 12.3 7.4 12.5 6.7 12.5 3.6 5.0 21.5 20.0 1.055 1.024 1.038 1.020 1.032 1.023 1.037 1.038 1.017 1.016 10.5 6.47 10 1.030 .0123 10 Dosa 7.5 7.0 7.0 7.0 7.0 7.0 6.5 7.5 7.0 6.5 7.0 .3 10 Dosa 7.0 7.0 6.5 7.0 7.0 7.0 7.0 7.0 6.5 7.0 6.9 .2 10 ppm ppm a Sample was overflowing at the time of collection. 4152 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 8 Dose Level: 0 C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 30 100 300 0 >2000 100 300 100 >2000 100 - Group: 9 Dose Level: 1 C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 300 100 300 100 300 30 300 300 100 300 - - Dosage Unit: ppm ----------------+ --- Dosage Unit: ppm +-+ - +++ ------+---+-+ ----- - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4153 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 8 C92195 C92199 C92205 C92211 C92223 C92230 C92238 C92246 C92247 C92253 Group: 9 C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 Dose Level: 0 00 00 00 00 01 01 00 30 01 00 Dose Level: 1 00 31 01 00 11 01 00 40 00 01 Dosage Unit:: ppm 02 0 010 02 0 110 1 2 1G1H 110 010 12 0 1 1 1H 010 Dosage Unit: ppm 1 1 1G 110 1 1 1G1H 010 12 0 010 110 02 0 010 1 1 1G 4D 2D 3D 0 4D 4D 0 2B4D 0 3D 4D 4D 0 1D 4D 4D 4D 4D 0 0 BJ BJ BJ AJ BJ BJ BJ BJ BJ BJ CJ BJ BJ BJ BJ BJ BJ BJ BJ BJ Q P PQ Q PQ Q Q P PQ Q PQ PQ P Q PQ Q PQ Q Q PQ Covance 6329-212 Covance 6329-228 3M T-6316.1 4154 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U VOL ML SP GR U PH Group: 8 C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 MEAN S.D. N Group: 9 C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 MEAN S.D. N Dose Level: 0 49.0 a 4.0 16.2 8.0 25.4 20.0 49.0 a 3.0 49.2 a 48.5 a 1.004 1.023 1.021 1.024 1.012 1.009 1.004 1.032 1.002 1.005 27.2 19.88 10 1.014 .0106 10 Dose Level: 1 2.6 8.5 13.4 18.7 4.6 38.9 12.0 13.3 8.3 10.0 1.039 1.018 1.017 1.011 1.025 1.006 1.016 1.013 1.026 1.016 13.0 10.19 10 1.019 .0093 10 Dosag 6.5 6.5 6.5 6.5 6.5 7.0 6.5 6.0 6.5 6.5 6.5 .24 10 Dosag 6.5 6.0 6.5 6.5 7.0 7.0 6.5 6.5 6.5 6.0 6.5 .33 10 ppm ppm a Sample was overflowing at the time of collection. 4155 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U PRO MG/DL U GLU U KET U BILI U BLOOD UROBILI Group: 8 C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 Group: 9 C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 Dose Level: 0 100 100 300 10 10 10 030 00- Dose Level: 1 100 100 10 10 10 10 30 300 30 10 - Dosage Unit: ppm --------------------- Dosage Unit: ppm ----+ ------------- - +++ - - Covance 6329-212 Covance 6329-228 3M T-6316.1 4156 ANIMAL NUMBER Appendix 5 Individual Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC WBC EPITH BACT CASTS CRYSTALS PER HPF PER. HPF PER HPF PER HPF PER LPF PER LPF1 URINE COMMENTS APP1 Group: 8 C92341 C92356 C92357 C92365 C92372 C92376 C92388 C92389 C92390 C92395 Group: 9 C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 Dose Level: 0 00 01 01 01 00 00 00 01 00 00 Dose Level: 1 04 01 00 00 00 00 00 00 00 41 Dosage Unit: ppm 1 2 1G 1 2 1G 1 1 2G 110 030 010 110 110 010 12 0 Dosage Unit: ppm 12 0 1 2 1G 010 110 130 12 0 110 1 1 1G 110 110 0 1B 0 0 0 0 0 0 0 0 1B 0 0 0 1B4D 0 0 0 0 0 AJ BJ BJ BJ BJ AJ AJ BJ AJ AJ BJ BJ BJ BJ BJ AJ BJ BJ BJ BJ P P P P P - P P P P P P - Covance 6329-212 Covance 6329-228 3M T-6316.1 4157 Appendix 5 Individual Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 a C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 a C92263 C92265 C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Dose Level: 0 33 111 12 29 42 254 57 129 13 42 10 18 7 16 18 73 13 35 34 93 24 16.7 10 80 72. 9 10 Dose Level: 1 25 74 8 25 24 69 33 96 41 74 52 183 30 115 20 80 49 124 15 50 30 14.3 10 89 43. 9 10 Dosage Unit: ppm .34 1.14 .37 .90 .24 1.42 .47 1.07 .43 1.38 .42 .76 -- .25 1.00 .31 .84 .36 .99 .35 .079 9 1.06 .227 9 Dosage Unit: ppm .40 1.20 -- .55 1.59 .41 1.19 .83 1.50 .29 1.01 .52 2.00 .54 2.17 .47 1.18 .59 1.97 .51 .151 9 1.53 .425 9 a Unable to calculate excretion due to inaccurate urine volume. 4158 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 8 C92331 C92333 a C92334 C92336 C92337 C92338 C92339 C92340 C92341 C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 a C92406 C92407 C92408 C92409 C92410 a C92411 MEAN S.D. N Dose Level: 0 20 60 69 40 103 28 103 33 64 42 95 29 82 18 45 32 131 10 71 26 12.0 10 76 34. 6 10 Dose Level: 1 18 56 6 11 27 112 6 15 14 27 45 126 16 40 20 32 5 15 18 78 18 12.0 10 51 41. 3 10 Dosage Unit: ppm .18 .54 -- .17 .43 .14 .53 .34 .66 .21 .48 .47 1.33 .21 .54 .17 .69 .07 .52 .22 .119 9 .64 .273 9 Dosage Unit: ppm .40 1.25 .24 .45 .12 .52 -- .08 .15 .09 .25 .28 .71 .37 .59 -.14 .58 .22 .126 8 .56 .333 8 a Unable to calculate excretion due to inaccurate urine volume. 4159 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 8 C92191 C92193 C92194 C92195 C92197 C92199 C92200 C92201 C92202 C92203 MEAN S.D. N Group: 9 C92261 C92262 C92263 C92265 a C92266 C92267 C92268 C92269 C92270 C92271 MEAN S.D. N Dose Level : 0 17 84 24 127 20 190 38 186 3 19 10 70 22 92 15 120 15 146 14 90 18 9.3 10 112 52. 8 10 Dose Level : 1 48 142 16 166 8 82 8 41 42 138 53 476 27 166 10 72 41 160 5 66 26 18.7 10 151 123. 3 10 Dosage Unit: ppm .17 .84 .20 1.05 .16 1.52 .21 1.02 .13 .85 .18 1.22 .21 .87 .09 .70 .11 1.05 .31 1.98 .18 .062 10 1.11 .383 10 Dosage Unit: ppm .43 1.28 .12 1.29 .10 1.01 -- .34 1.10 .08 .76 .32 1.96 .23 1.66 .38 1.49 .12 1.53 .24 .135 9 1.34 .363 9 a Unable to calculate excretion due to inaccurate 4160 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 8 C92331 C92333 C92334 C92336 a C92337 C92338 C92339 C92340 C92341 a C92342 MEAN S.D. N Group: 9 C92401 C92403 C92404 C92405 C92406 QS C92407 C92408 C92409 C92410 C92411 MEAN S.D. N Dose Level: 0 16 134 24 124 47 253 4 62 9 65 42 232 3 24 7 56 7 18 3 43 16 16.3 10 101 83. 6 10 Dose Level: 1 13 115 9 109 5 24 7 55 -- 1 10 5 21 26 136 11 109 9 55 10 7.1 9 70 47. 4 9 Dosage Unit: ppm .11 .94 .06 .32 .11 .58 -- .07 .54 .11 .60 .08 .65 .05 .38 -- .06 .86 .08 .025 8 .61 .212 8 Dosage Unit: ppm .17 1.50 .13 1.55 .21 1.00 .07 .54 -- .05 .46 .22 .91 .13 .68 .11 1.09 .14 .87 .14 .057 9 .96 .383 9 a Unable to calculate excretion due to inaccurate urine volume. 4161 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 8 C92195 C92199 C92205 C92211 a C92223 C92230 C92238 C92246 C92247 C92253 MEAN S.D. N Group: 9 C92267 C92274 C92286 C92287 C92288 C92303 C92315 C92317 C92320 C92324 MEAN S.D. N Dose Level: 0 16 88 19 105 26 158 2 10 13 183 41 182 8 124 42 254 14 200 21 123 20 13.0 10 143 68. 2 10 Dose Level: 1 64 361 14 124 35 211 34 104 17 174 21 111 14 207 42 212 8 72 8 70 26 17.9 10 165 88. 6 10 Dosage Unit: ppm .24 1.34 .19 1.03 .47 2.84 -- .09 1.28 .42 1.87 .11 1.76 .26 1.60 .13 1.84 .15 .90 .23 .135 9 1.61 .579 9 Dosage Unit: ppm .21 1.19 .17 1.53 .26 1.56 .42 1.30 .11 1.17 .26 1.39 .05 .75 .21 1.06 .17 1.55 .16 1.40 .20 .100 10 1.29 .256 10 a Unable to calculate excretion due to inaccurate urine volume. 4162 Covance 6329-212 Covance 6329-228 3M T-6316.1 Appendix 5 Individual Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ANIMAL NUMBER U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL Group: 8 C92341 a C92356 C92357 C92365 C92372 C92376 C92388 a C92389 C92390 a C92395 a MEAN S.D. N Group: 9 C92406 C92407 C92411 C92418 C92422 C92424 C92433 C92438 C92441 C92449 MEAN S.D. N Dose Level: 0 11 51 15 77 26 115 30 133 20 46 11 39 2 17 10 138 3 12 4 17 13 9.6 10 64 48. 6 10 Dose Level: 1 51 298 10 75 25 86 8 59 34 295 7 20 14 69 8 53 31 164 10 59 20 14.9 10 118 101. 1 10 Dosage Unit: ppm -- .06 .31 .42 1.86 .24 1.06 .51 1.17 .22 .78 -.03 .41 -- -- .25 .191 6 .93 .569 6 Dosage Unit: ppm .13 .77 .08 .64 .34 1.15 .15 1.10 .16 1.36 .27 .78 .17 .83 .11 .70 .26 1.36 .10 .59 .18 .085 10 .93 .290 10 a Unable to calculate excretion due to inaccurate urine volume. 4163 Covance 6329-212 Covance 6329-228 3M T-6316.1 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta Covance 6329-212 FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-228 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e X I o f X II Page 4164 of 4873 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME XI OF XII CONTENTS Page A P P E N D I X 6 ......................................................................................................................................................................................4 1 6 6 I n d i v i d u a l A n a t o m i c P a t h o l o g y D a t a .................................................................................................................... 4 1 6 7 4165 APPENDIX 6 In d iv id u a l A n a to m ic P a th o lo g y D a ta Covance 6329-212 Covance 6329-228 3M T-6316.1 4166 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 1 ANIMAL NUMBER: C92191 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 882.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:55 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 64; 135 X 100MM; VERIFIED AT NECROPSY OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED SKIN (SK) : >NOTE:>COLLECTED FROM RIGHT INGUINAL REGION DUE TO MM MASS HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI FOCAL -HYPERPLASIA, FOLLICULAR CELL,-SLIGHT, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4167 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 2 ANIMAL NUMBER: C92191 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 882.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:55 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 130 X 100 X 65 MM, DARK RED, RED, TAN, GRAY, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN COLON (CO) : -PARASITISM,-PRESENT DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT MAMMARY, MALE (MM) : -B-FIBROADENOMA,-NEOPLASM PRESENT 4168 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 3 ANIMAL NUMBER: C92191 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 882.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:55 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4169 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 4 ANIMAL NUMBER: C92192 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 313.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:55 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 10.9046 ------------------------------------------- ------------------------------------ 3.4806 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4170 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 5 ANIMAL NUMBER: C92192 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 313.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:55 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4171 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 6 ANIMAL NUMBER: C92193 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:42 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RAISED AREA(S); ANTERIOR ASPECT: SINGLE DARK RED RAISED AREA, 2 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT BRAIN (BR) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY,-MODERATE, FOCAL -VACUOLATION,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4172 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 7 ANIMAL NUMBER: C92193 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:42 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DARK RED, DRY CRUSTED AREA, 5 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN FOOT/FOOTPAD (FP) : -EROSION,-MINIMAL, FOCAL -FIBROSIS,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 4173 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 8 ANIMAL NUMBER: C92193 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 645.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:42 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4174 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 9 ANIMAL NUMBER: C92194 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 824.1 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 14:54 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; DILATED PUPIL; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LUNG (LU) : -DIFFUSELY RED; ALL LOBES KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES, DIFFUSE: MULTIPLE DARK RED, PINPOINT FOCI; COLLECTED ON ROUTINE SECTIONS -DIFFUSELY LIGHT; ALL LOBES: TAN -CYST(S); ALONG MARGIN OF LEFT LATERAL LOBE: TWO CLEAR FLUID FILLED CYSTS, ONE IS 3 MM IN DIAMETER, THE OTHER IS 5 MM IN DIAMETER; BOTH ARE COLLECTED ON THE ROUTINE SECTION -ROUGH SURFACE, DIFFUSE; ALL LOBES HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL LIVER (LI) : -CYST,-PRESENT -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL 4175 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 10 ANIMAL NUMBER: C92194 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 824.1 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 14:54 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 2 MM PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: TAN SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 18 X 10 X 3 MM PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL -THROMBUS, ATRIAL,-MODERATELY SEVERE, FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATE, DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL DEATH COMMENT (DC) : -HEPATOCELLULAR NECROSIS,-PRESENT 4176 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 11 ANIMAL NUMBER: C92194 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 824.1 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 14:54 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MEDIASTINAL (ML) : -DIFFUSELY RED -LARGE; UP TO 6 X 5 X 5 MM; APPEARS CYSTIC (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE -FLUID-THORACIC CAVITY; CONTAINS A LARGE AMOUNT OF CLEAR FLUID LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4177 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 12 ANIMAL NUMBER: C92194 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 824.1 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 14:54 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (AS), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (HG), 4178 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 13 ANIMAL NUMBER: C92195 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0220 .0313 2.0881 1.9642 2.0438 15.3839 1.6924 .7077 1.6067 1.6857 .0261 .0194 .0034 % .0049 % .3265 % .3071 % .3195 % 2.4052 % .2646 % .1106 % .2512 % .2636 % .0041 % .0030 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0105 .0150 1.0000 .9407 .9788 7.3674 .8105 .3389 .7695 .8073 .0125 .0093 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4179 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 14 ANIMAL NUMBER: C92195 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 639.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4180 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 15 ANIMAL NUMBER: C92196 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 299.0 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:47 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 9.9178 ------------------------------------------- ------------------------------------ 3.3170 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4181 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 16 ANIMAL NUMBER: C92196 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 299.0 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:47 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4182 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 17 ANIMAL NUMBER: C92197 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 521.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:21 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES, UP TO 1 MM IN DIAMETER LIVER (LI) : -MASS(ES); RIGHT MEDIAN LOBE: SINGLE MASS, 27 X 28 X 15 MM, BROWN, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (HEPATIC) NORMAL (CAP 23) TESTIS (TE) : -SMALL; RIGHT: 24 X 15 X 7 MM (LEFT IS 25 X 16 X 11 MM); RIGHT IS SOFT SKIN (SK) : -RAISED AREA(S); MID DORSAL LUMBAR REGION: SINGLE TAN AREA, 3 X 2 MM HISTOPATHOLOGY KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE >NOTE:>RIGHT TESTIS SKIN (SK) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL 4183 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 18 ANIMAL NUMBER: C92197 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 521.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:21 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY JOINT, OTHER (JT) : -LARGE; LEFT TARSAL JOINT: UP TO 20 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -BONE MARROW SMEAR TAKEN SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT BRAIN (BR) : -MINERALIZATION,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : >UNREMARKABLE >NOTE:>HEPATIC LYMPH NODE JOINT, OTHER (JT) : -EDEMA,-MODERATE, MULTI-FOCAL -EXOSTOSES,-MODERATE, FOCAL -INFLAMMATION, ACUTE, SYNOVIAL,-SLIGHT, MULTI-FOCAL 4184 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 19 ANIMAL NUMBER: C92197 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 521.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:21 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4185 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 20 ANIMAL NUMBER: C92198 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/98 STUDY DAY OF DEATH: 75 STUDY WEEK OF DEATH: 11 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/98 13:54 PROSECTOR: TROY ELLENBECKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE WHITE, TAN AND HARD CALCULI, UP TO 13 X 10 X 8 MM -THICKENED WALL; DIFFUSE -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM URETER (UE) : -LARGE; BILATERAL: UP TO 2 MM IN DIAMETER (CAP 18) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -URINARY BLADDER-LARGE LUMEN; 50 X 30 X 20 MM; CONTAINS A BROWN SEMIFLUID -AUTOLYSIS-SEVERE; ENTIRE GI TRACT HISTOPATHOLOGY KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,SEVERE, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -CYSTITIS,-PRESENT URETER (UE) : -DILATATION,-MODERATE, DIFFUSE 4186 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 21 ANIMAL NUMBER: C92198 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/19/98 STUDY DAY OF DEATH: 75 STUDY WEEK OF DEATH: 11 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/19/98 13:54 PROSECTOR: TROY ELLENBECKER RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4187 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 22 ANIMAL NUMBER: C92199 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 635.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:03 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0297 .0343 2.3690 2.0154 1.9204 16.0651 2.1731 .8156 1.7522 1.6697 .0238 .0341 .0047 % .0054 % .3727 % .3171 % .3021 % 2.5275 % .3419 % .1283 % .2757 % .2627 % .0037 % .0054 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0125 .0145 1.0000 .8507 .8106 6.7814 .9173 .3443 .7396 .7048 .0100 .0144 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL STOMACH, GL (ST) : -EDEMA,-SLIGHT, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT MULTI 4188 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 23 ANIMAL NUMBER: C92199 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 635.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:03 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4189 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 24 ANIMAL NUMBER: C92200 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 673.4 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 9:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); TAIL-PROXIMAL; WEEK 74; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 5 X 5 X 7 MM, FIRM, RED, DARK RED, TAN AND LOBULATED HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CYST,-SLIGHT, FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4190 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 25 ANIMAL NUMBER: C92200 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 673.4 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 9:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TAIL (TI) : -RAISED AREA(S); NEAR BASE OF TAIL: SINGLE TAN FIRM AREA, 4 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL RECTUM (RE) : >TISSUE MISSING PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT TAIL (TI) : -B-FIBROMA,-NEOPLASM PRESENT 4191 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 26 ANIMAL NUMBER: C92200 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/21/99 STUDY DAY OF DEATH: 534 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 673.4 GRAMS DATE AND TIME OF NECROPSY: 09/21/99 9:55 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4192 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 27 ANIMAL NUMBER: C92201 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 662 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 682.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:33 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; LIMITED USE; LIMBS-HIND; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK RED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT BRAIN (BR) : >UNREMARKABLE HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4193 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 28 ANIMAL NUMBER: C92201 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/27/00 STUDY DAY OF DEATH: 662 STUDY WEEK OF DEATH: 95 TERMINAL BODY WEIGHT: 682.3 GRAMS DATE AND TIME OF NECROPSY: 01/27/00 12:33 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SM), 4194 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 29 ANIMAL NUMBER: C92202 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/25/99 STUDY DAY OF DEATH: 599 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/25/99 7:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, RED, DARK RED, TAN AND SOFT MAMMARY, MALE (MM) : -CYST(S); BILATERAL PECTORAL REGION: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 19) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 4195 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 30 ANIMAL NUMBER: C92202 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/25/99 STUDY DAY OF DEATH: 599 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/25/99 7:24 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4196 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 31 ANIMAL NUMBER: C92203 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAW-HIND-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -LARGE VESSELS; UP TO 2 MM IN DIAMETER (CAP 24) PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS UP TO 2 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -CYST,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -HYPERTROPHY, VASCULAR,-MODERATELY SEVERE, MULTI-FOCAL -INFLAMMATION, VASCULAR,-MINIMAL, MULTI FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE, DIFFUSE TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, MULTI FOCAL 4197 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 32 ANIMAL NUMBER: C92203 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 7 MM IN DIAMETER -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; ALL REGIONS DIFFUSE LN, RENAL (RL) : -LARGE; BILATERAL: EACH IS 6 X 5 X 4 MM (CAP 22) -DIFFUSELY RED; BILATERAL: DARK RED TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION,-MODERATE, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, RENAL (RL) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4198 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 33 ANIMAL NUMBER: C92203 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL: EACH IS 7 X 5 X 2 MM (CAP 23) JOINT, OTHER (JT) : -LARGE; LEFT TARSAL: UP TO 15 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, RENAL (RL) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL JOINT, OTHER (JT) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -EXOSTOSES,-MODERATELY SEVERE, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE, SYNOVIAL,-MODERATE, MULTI-FOCAL 4199 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 34 ANIMAL NUMBER: C92203 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SB), 4200 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 35 ANIMAL NUMBER: C92204 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/27/99 13:56 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -GRANULAR MATERIAL; RIGHT, WITHIN PELVIS: MULTIPLE TAN AND GRAY GRANULES, PINPOINT AND UP TO 1 MM IN DIAMETER LIVER (LI) : -LARGE; LEFT LATERAL LOBE BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -LARGE; 71 X 19 X 12 MM PITUITARY (PI) : -MASS(ES); 6 X 6 X 4 MM, DARK RED, LOBULATED AND SOFT; MASS ADHERED TO HYPOTHALAMUS OF BRAIN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL SPINAL CORD (SC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL SPLEEN (SP) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SEVERE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4201 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 36 ANIMAL NUMBER: C92204 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/27/99 13:56 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC PROSTATE (PR) : >UNREMARKABLE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT 4202 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 37 ANIMAL NUMBER: C92204 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/27/99 13:56 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP) 4203 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 38 ANIMAL NUMBER: C92205 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 832.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:32 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0471 .0298 2.4066 2.5383 2.6192 20.3350 2.3955 1.0579 1.8842 1.9211 .0299 .0350 .0057 % .0036 % .2891 % .3049 % .3147 % 2.4429 % .2878 % .1271 % .2264 % .2308 % .0036 % .0042 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0196 .0124 1.0000 1.0547 1.0883 8.4497 .9954 .4396 .7829 .7983 .0124 .0145 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -HEMORRHAGE,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI FOCAL HARDERIAN GLAND (HG) : -ONE EXAMINED,-PRESENT 4204 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 39 ANIMAL NUMBER: C92205 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 832.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:32 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN RECTUM (RE) : -PARASITISM,-PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4205 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 40 ANIMAL NUMBER: C92205 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 832.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:32 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4206 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 41 ANIMAL NUMBER: C92206 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 531.2 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 11:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -THICKENED WALL; MUCOSA, NEAR JUNCTION OF NONGLANDULAR: TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : >UNREMARKABLE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4207 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 42 ANIMAL NUMBER: C92206 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 531.2 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 11:22 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4208 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 43 ANIMAL NUMBER: C92207 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:15 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 11.1487 ------------------------------------------- ------------------------------------ 3.5258 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4209 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 44 ANIMAL NUMBER: C92207 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 316.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:15 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4210 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 45 ANIMAL NUMBER: C92208 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1038.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:47 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSALTHORACIC-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS TESTIS (TE) : -SMALL; BILATERAL: EACH IS 9 X 9 X 20 MM -CONTAINS FLUID; BILATERAL: CLEAR FLUID HISTOPATHOLOGY KIDNEY (KD) : -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, DIFFUSE -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, MULTI FOCAL SKIN (SK) : -INFLAMMATION, ACUTE,-MINIMAL, FOCAL 4211 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 46 ANIMAL NUMBER: C92208 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1038.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:47 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SKIN (SK) : -CRUSTED AREA(S); RIGHT LATERAL THORACIC AND ABDOMINAL REGIONS: MULTIPLE DRY, RED CRUSTED AREAS, UP TO 3 X 4 MM (CAP 22) BRAIN (BR) : -MINERALIZATION,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL PROSTATE (PR) : -EDEMA,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 4212 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 47 ANIMAL NUMBER: C92208 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1038.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:47 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MATERIAL -ANIMAL OBESE -BONE MARROW SMEAR TAKEN DRY, BROWN SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4213 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 48 ANIMAL NUMBER: C92208 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1038.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:47 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4214 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 49 ANIMAL NUMBER: C92209 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 X 5 X 3 MM, RED, TAN AND FIRM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 2 X 1 MM HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE PANCREAS (PA) : -HYPERTROPHY, VASCULAR,-MODERATE, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : ^UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, ACUTE,-MINIMAL, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT MULTI 4215 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 50 ANIMAL NUMBER: C92209 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -BONE MARROW SMEAR TAKEN 4216 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 51 ANIMAL NUMBER: C92209 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 527.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4217 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 52 ANIMAL NUMBER: C92210 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/23/00 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 02/23/00 10:41 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 10 X 8 MM; BOTH ARE SOFT BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 8 X 7 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE, DIFFUSE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, FOCAL -VACUOLATION,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4218 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 53 ANIMAL NUMBER: C92210 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/23/00 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 02/23/00 10:41 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE BROWN ERODED AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA, DIFFUSE PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 13 X 5 X 3 MM LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 20) LN, LUMBAR/ILIAC (LM) : -MOTTLED; BILATERAL: RED AND TAN (CAP 21) MAMMARY, MALE (MM) : -CYST(S); BILATERAL, PECTORAL REGIONS: MULTIPLE WHITE CREAMY SEMIFLUID FILLED CYSTS, UP TO 2 MM IN DIAMETER (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS STOMACH, GL (ST) : ^UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-SLIGHT, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : >UNREMARKABLE LN, LUMBAR/ILIAC (LM) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 4219 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 54 ANIMAL NUMBER: C92210 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/23/00 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 02/23/00 10:41 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; PERIANAL: DRY RED MATERIAL; POSSIBLY CONSISTENT WITH STAINS (PERINEAL REGION) AS NOTED IN CLINICAL OBSERVATIONS -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY YELLOW MATERIAL 4220 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 55 ANIMAL NUMBER: C92210 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/23/00 STUDY DAY OF DEATH: 689 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 02/23/00 10:41 PROSECTOR: JOHN S. HALFORD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4221 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 56 ANIMAL NUMBER: C92211 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 627.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0295 .0271 2.4300 2.1992 2.2819 17.6271 2.1850 .9756 1.8440 1.7913 .0192 .0168 .0047 % .0043 % .3872 % .3504 % .3636 % 2.8087 % .3482 % .1554 % .2938 % .2854 % .0031 % .0027 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0121 .0112 1.0000 .9050 .9391 7.2540 .8992 .4015 .7588 .7372 .0079 .0069 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4222 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 57 ANIMAL NUMBER: C92211 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 627.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4223 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 58 ANIMAL NUMBER: C92212 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:14 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 1 MM IN DIAMETER TESTIS (TE) : -SMALL; BILATERAL: EACH IS 18 X 9 X 8 MM; BOTH ARE SOFT BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE -THROMBUS,-MODERATELY SEVERE, FOCAL LIVER (LI) : >UNREMARKABLE PANCREAS (PA) : -THROMBUS,-MODERATELY SEVERE, FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL, DIFFUSE TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 4224 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 59 ANIMAL NUMBER: C92212 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:14 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 MM IN DIAMETER, RED, TAN AND SOFT STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM -THICKENED WALL; MUCOSA, DIFFUSE STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA, DIFFUSE -EROSION/ULCERATION; MUCOSA: MULTIPLE RED AND TAN ERODED AREAS, UP TO 5 MM IN DIAMETER DUODENUM (DU) : -THICKENED WALL; DIFFUSE; COLLECTED ON ROUTINE SECTION SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 15 X 5 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; RED, MOIST MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL, FOCAL STOMACH, NONGL (SU) : -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL DUODENUM (DU) : >UNREMARKABLE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : ^UNREMARKABLE DEATH COMMENT (DC) : -PYELONEPHRITIS,-PRESENT 4225 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 60 ANIMAL NUMBER: C92212 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 372.6 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:14 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4226 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 61 ANIMAL NUMBER: C92213 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 03/27/99 14:50 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -MASS(ES); LEFT: SINGLE MASS, 35 X 20 X 10 MM, RED, DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED WITH LEFT KIDNEY; REGIONAL LN (RENAL) NOT IDENTIFIED TESTIS (TE) : -SMALL; RIGHT: 17 X 11 X 7 MM (FOR COMPARISON, LEFT IS 20 X 14 X 10 MM) HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL -N-TRANSITIONAL CELL CARCINOMA,NEOPLASM PRESENT KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL -THROMBUS,-MODERATE, MULTI-FOCAL -I-TRANSITIONAL CELL CARCINOMA,NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -SPERMATOCELE,-MODERATE, FOCAL EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, MULTI-FOCAL 4227 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 62 ANIMAL NUMBER: C92213 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 03/27/99 14:50 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE RED AND DARK RED AREAS, UP TO 2 X 1 MM PROSTATE (PR) : -GELATINOUS; DIFFUSE: TAN AND CLEAR SEMINAL VESICLES (SV) : -MASS(ES); BILATERAL, INVOLVING ALL OF BOTH ORGANS: SINGLE MASS, 65 X 55 X 40 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN EYE (EY) : -INFLAMMATION, ACUTE, CILIARY,-MINIMAL, MULTI-FOCAL -ULCERATION, CORNEAL,-MODERATE, FOCAL STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -I-TRANSITIONAL CELL CARCINOMA,NEOPLASM PRESENT SEMINAL VESICLES (SV) : -M-TRANSITIONAL CELL CARCINOMA,NEOPLASM PRESENT DEATH COMMENT (DC) : -TRANSITIONAL CELL CARCINOMA,-PRESENT 4228 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 63 ANIMAL NUMBER: C92213 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: 506.4 GRAMS DATE AND TIME OF NECROPSY: 03/27/99 14:50 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SE), 4229 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 64 ANIMAL NUMBER: C92214 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 600.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:59 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN -LARGE; BILATERAL: EACH IS 27 X 17 X 14 MM LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: LIGHT RED >NOTE:>TRIMMING COMMENT: RIGHT MEDIAN LOBE OF LIVER MISSING AT TIME OF TRIMMING HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF BOTH VENTRICLES: MULTIPLE GRAY INDISTINCT AREAS ADRENAL, CORTEX (AC) : -DIFFUSELY RED; BILATERAL: DARK RED STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM HISTOPATHOLOGY KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, TUBULAR,-SLIGHT, MULTI FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL -THROMBUS,-MODERATELY SEVERE, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL HEART (HT) : >UNREMARKABLE ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : -EDEMA,-SLIGHT, FOCAL STOMACH, NONGL (SU) : -ACANTHOSIS,-SLIGHT, DIFFUSE -HYPERKERATOSIS, NONGLANDULAR MUCOSA,SLIGHT, DIFFUSE 4230 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 65 ANIMAL NUMBER: C92214 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 600.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:59 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE TAN, HARD CALCULI, UP TO 7 MM IN DIAMETER -THICKENED WALL; DIFFUSE -DIFFUSELY RED; RED AND DARK RED -SEMIFLUID MATERIAL; WITHIN LUMEN: CONTAINS A YELLOWISH-BROWN SEMIFLUID MATERIAL SEMINAL VESICLES (SV) : -SMALL; RIGHT: 15 X 6 X 2 MM (LEFT IS 25 X 10 X 3 MM) URETER (UE) : -LARGE; BILATERAL: EACH IS 1 MM IN DIAMETER (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DRY, YELLOW MATERIAL -URINARY BLADDER-LARGE LUMEN; 45 X 27 X 27 MM -DISCHARGE-NASAL; DRY, RED MATERIAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATELY SEVERE, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -CYSTITIS,-PRESENT URETER (UE) : -DILATATION,-MODERATE, DIFFUSE 4231 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 66 ANIMAL NUMBER: C92214 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 600.8 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:59 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE,STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN,MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE,STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN,MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (DU), 4232 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 67 ANIMAL NUMBER: C92215 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 555.0 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-CERVICAL; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL AND CERVICAL REGIONS, HAIRCOAT: BROWN HISTOPATHOLOGY KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT 4233 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 68 ANIMAL NUMBER: C92215 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 555.0 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 10:21 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4234 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 69 ANIMAL NUMBER: C92216 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 298.1 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:55 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 11.8239 ------------------------------------------- ------------------------------------ 3.9664 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4235 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 70 ANIMAL NUMBER: C92216 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 298.1 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:55 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4236 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 71 ANIMAL NUMBER: C92217 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 1135.5 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:40 PROSECTOR: LYNN HORNER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 63; 140 X 100MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RAISED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: SINGLE RAISED AREA, 15 X 14 X 7 MM; COLLECTED ON ROUTINE SECTION SPLEEN (SP) : -LARGE; 6 5 X 1 9 X 6 MM PITUITARY (PI) : -LARGE; 6 X 6 X 3 MM, APPEARS SWOLLEN STOMACH, GL (ST) : -THICKENED WALL; MUCOSA HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL STOMACH, GL (ST) : >UNREMARKABLE 4237 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 72 ANIMAL NUMBER: C92217 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 1135.5 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:40 PROSECTOR: LYNN HORNER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); RIGHT ABDOMINAL REGION: 150 X 120 X 75 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; PORTIONS COLLECTED; OVERLYING SKIN HAS A DARK RED, DRY, CRUSTED AREA, 50 X 35 MM; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER NOT FOUND AT NECROPSY DUE TO LARGE MAMMARY GLAND MASS PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -FIBROMA,-PRESENT MAMMARY, MALE (MM) : >TISSUE MISSING >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS FIBROMA 4238 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 73 ANIMAL NUMBER: C92217 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/04/99 STUDY DAY OF DEATH: 547 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 1135.5 GRAMS DATE AND TIME OF NECROPSY: 10/04/99 14:40 PROSECTOR: LYNN HORNER RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4239 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 74 ANIMAL NUMBER: C92218 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 382.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:15 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; MISSING; TEETH; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -MASS(ES); RIGHT: SINGLE MASS, 8 X 8 X 4 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (RENAL) NORMAL (CAP 22) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 12 X 10 MM, DARK RED, GRAY AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE DARK BROWN ERODED AREAS, UP TO 4 X 2 MM HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -M-CARCINOMA, TUBULAR CELL,-NEOPLASM PRESENT LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL 4240 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 75 ANIMAL NUMBER: C92218 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 382.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:15 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DARK RED, CRUSTED AREA, 8 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -ANIMAL THIN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATE, DIFFUSE PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -EROSION,-SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL 4241 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 76 ANIMAL NUMBER: C92218 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 382.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:15 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), LN, RENAL (RL), HEMATO NEOPLASIA (HN) 4242 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 77 ANIMAL NUMBER: C92219 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 990.3 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 15:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; TAIL-DISTAL; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; OTHER; ANIMAL IS CHEWING ON DISTAL PORTION OF TAIL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN LIVER (LI) : -MOTTLED; ALL LOBES: LIGHT BROWN AND TAN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI FOCAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL -HEMORRHAGE,-MODERATE, FOCAL PITUITARY (PI) : >UNREMARKABLE 4243 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 78 ANIMAL NUMBER: C92219 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 990.3 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 15:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE PROSTATE (PR) : -LARGE; 28 X 25 X 10 MM -DIFFUSELY RED; DARK RED SEMINAL VESICLES (SV) : -LARGE; BILATERAL: EACH IS 30 X 15 X 8 MM; BOTH ARE FILLED WITH A DARK RED FLUID TAIL (TI) : -CRUSTED AREA(S); DISTAL ONE THIRD: RED, DARK RED AND TAN INDISTINCT AREAS; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -URINARY BLADDER-LARGE LUMEN; UP TO 20 MM IN DIAMETER; FILLED WITH A DARK RED FLUID -ANIMAL OBESE STOMACH, GL (ST) : ^UNREMARKABLE URINARY BLADDER (UB) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL PROSTATE (PR) : >UNREMARKABLE MULTI SEMINAL VESICLES (SV) : -EDEMA,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT TAIL (TI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4244 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 79 ANIMAL NUMBER: C92219 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/03/99 STUDY DAY OF DEATH: 607 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 990.3 GRAMS DATE AND TIME OF NECROPSY: 12/03/99 15:08 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -GI TRACT-DARK FLUID CONTENT; ILEUM, JEJUNUM, CECUM AND COLON: DARK GREEN SEMIFLUID THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SB), 4245 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 80 ANIMAL NUMBER: C92220 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 538 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/25/99 14:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 1 X 1 X 2 MM HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE HEART (HT) : -DEGENERATION/NECROSIS,-MODERATE, MULTI FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL STOMACH, GL (ST) : -EROSION,-SLIGHT, FOCAL 4246 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 81 ANIMAL NUMBER: C92220 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 538 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/25/99 14:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 1 X 3 MM AORTA (AO) : -LARGE; DIFFUSE: UP TO 5 MM IN DIAMETER (CAP 19) -FIRM; DIFFUSE (CAP 19) GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -AUTOLYSIS-SEVERE -DISCHARGE-OCULAR; LEFT: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL STOMACH, GL (ST) : -MINERALIZATION,-MODERATELY SEVERE, MULTI-FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC COLON (CO) : >AUTOLYTIC DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT AORTA (AO) : -MINERALIZATION,-MODERATELY SEVERE, MULTI-FOCAL 4247 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 82 ANIMAL NUMBER: C92220 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/25/99 STUDY DAY OF DEATH: 538 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/25/99 14:15 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4248 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 83 ANIMAL NUMBER: C92221 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 368.2 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN LIVER (LI) : -MASS(ES); RIGHT MEDIAN LOBE: SINGLE MASS, 25 X 20 X 15 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED ON ROUTINE SECTION; REGIONAL LN (HEPATIC) NORMAL (CAP 19) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MODERATE, DIFFUSE TESTIS (TE) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,MODERATE, MULTI-FOCAL -VASCULARIZATION, CORNEAL,-MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL 4249 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 84 ANIMAL NUMBER: C92221 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 368.2 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; AND DRY -ANIMAL THIN : BILATERAL: DARK RED ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -HEPATOCELLULAR ADENOMA,-PRESENT LN, OTHER (LN) : ^UNREMARKABLE >NOTE:>HEPATIC LYMPH NODE 4250 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 85 ANIMAL NUMBER: C92221 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 368.2 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4251 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 86 ANIMAL NUMBER: C92222 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 802.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:35 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 95; 15 X 10MM ANTERIOR 10 X 10MM POSTERIOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS SKIN (SK) : -MASS(ES); DORSAL LUMBAR REGION: TWO MASSES, ONE IS 23 X 17 X 9 MM, THE OTHER IS 12 X 12 X 6 MM; BOTH ARE TAN AND FIRM; BOTH ARE SAME ON CUT SURFACE; BOTH COLLECTED; REGIONAL LN (LUMBAR) NORMAL (CAP 22) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HYPERPLASIA, C-CELL,-MODERATE, FOCAL SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT NERVE, SCIATIC (SN) : >TISSUE MISSING HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL MULTI 4252 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 87 ANIMAL NUMBER: C92222 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 802.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:35 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 5 X 4 X 3 MM, RED, DARK RED, TAN, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4253 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 88 ANIMAL NUMBER: C92222 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 802.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:35 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, LUMBAR/ILIAC (LM), HEMATO NEOPLASIA (HN) 4254 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 89 ANIMAL NUMBER: C92223 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 656.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:23 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0314 .0327 2.7369 2.5158 2.4904 23.3120 2.3498 1.0939 1.5705 1.5425 .0169 .0186 .0048 % .0050 % .4170 % .3833 % .3794 % 3.5515 % .3580 % .1667 % .2393 % .2350 % .0026 % .0028 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0115 .0119 1.0000 .9192 .9099 8.5177 .8586 .3997 .5738 .5636 .0062 .0068 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4255 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 90 ANIMAL NUMBER: C92223 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 656.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:23 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4256 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 91 ANIMAL NUMBER: C92224 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 716 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 8:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); DORSAL; WEEK 93; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; SWOLLEN; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -CRUSTED AREA(S); DORSAL THORACIC REGION: SINGLE RED, TAN, DRY, FIRM, RAISED AREA, 13 X 6 X 6 MM (CAP 23) ADRENAL, CORTEX (AC) : -RED FOCUS(I)/AREA(S); RIGHT: SINGLE DARK RED AREA, 3 MM IN DIAMETER PITUITARY (PI) : -DIFFUSELY RED; LEFT ANTERIOR ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL SKIN (SK) : -B-BASAL CELL TUMOR,-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : >UNREMARKABLE MULTI ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,- 2 NEOPLASMS PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC 4257 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 92 ANIMAL NUMBER: C92224 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 716 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 8:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); BILATERAL, HINDFEET: SINGLE RED, TAN MOIST ABRADED AREA ON EACH, LEFT IS 20 X 15 MM, RIGHT IS 12 X 9 MM; BOTH APPEAR SWOLLEN; BOTH COLLECTED LN, LUMBAR/ILIAC (LM) : -LARGE; BILATERAL, LUMBAR: EACH IS 13 X 6 X 4 MM (CAP 22) GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: BROWN AND MOIST DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT FOOT/FOOTPAD (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-SEVERE, MULTI-FOCAL -EXOSTOSES,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SEVERE, MULTI FOCAL LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-SLIGHT, MULTI FOCAL 4258 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 93 ANIMAL NUMBER: C92224 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/21/00 STUDY DAY OF DEATH: 716 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/21/00 8:04 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4259 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 94 ANIMAL NUMBER: C92225 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 494 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 447.4 GRAMS DATE AND TIME OF NECROPSY: 08/12/99 14:45 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 11 MM SOFT, RED, DARK RED, AND TAN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL MULTI 4260 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 95 ANIMAL NUMBER: C92225 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 494 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 447.4 GRAMS DATE AND TIME OF NECROPSY: 08/12/99 14:45 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DARK BROWN AND MOIST -DISCHARGE-OCULAR; DRY RED MATERIAL -ANIMAL THIN DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4261 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 96 ANIMAL NUMBER: C92225 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/12/99 STUDY DAY OF DEATH: 494 STUDY WEEK OF DEATH: 71 TERMINAL BODY WEIGHT: 447.4 GRAMS DATE AND TIME OF NECROPSY: 08/12/99 14:45 PROSECTOR: DENNIS HOFFMAN RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4262 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 97 ANIMAL NUMBER: C92226 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 496.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 9:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); AT JUNCTION OF MEDIAN LOBES: SINGLE TAN AREA, 3 X 2 MM (CAP 19) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 14 X 10 X 10 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL 4263 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 98 ANIMAL NUMBER: C92226 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 496.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 9:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DARK RED AND MOIST -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY -ANIMAL THIN DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4264 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 99 ANIMAL NUMBER: C92226 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/27/99 STUDY DAY OF DEATH: 631 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 496.8 GRAMS DATE AND TIME OF NECROPSY: 12/27/99 9:56 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4265 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 100 ANIMAL NUMBER: C92227 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 630.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -CYST(S); LEFT, CORTICAL SURFACE: SINGLE CLEAR FLUID FILLED CYST, 5 X 3 X 2 MM BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY LUNG (LU) : -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL KIDNEY (KD) : -CYST,-MODERATE, FOCAL -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL -PYELONEPHRITIS,-SLIGHT, MULTI-FOCAL LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI FOCAL BONE, FEMUR (FE) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -FIBROSIS,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4266 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 101 ANIMAL NUMBER: C92227 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 630.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 12 X 3 MM -THICKENED WALL; DIFFUSE DUODENUM (DU) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 7 X 3 MM; COLLECTED ON ROUTINE SECTION JEJUNUM (JE) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 7 X 5 MM; COLLECTED ON ROUTINE SECTION CECUM (CE) : -THICKENED WALL; DIFFUSE -RED FOCUS(I)/AREA(S); MUCOSA: SINGLE AREA, 12 X 10 MM PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL DUODENUM (DU) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL -ULCERATION,-MODERATELY SEVERE, MULTI FOCAL JEJUNUM (JE) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL -ULCERATION,-MODERATELY SEVERE, MULTI FOCAL ILEUM (IL) : -EROSION,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL CECUM (CE) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL 4267 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 102 ANIMAL NUMBER: C92227 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH:: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 630.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN CECUM (CE) : -ULCERATION,-MODERATELY SEVERE, MULTI FOCAL COLON (CO) : -EDEMA,-MINIMAL, FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MINIMAL, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL DEATH COMMENT (DC) : -ENTERITIS,-PRESENT 4268 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 103 ANIMAL NUMBER: C92227 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 630.9 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4269 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 104 ANIMAL NUMBER: C92228 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/25/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 423.5 GRAMS DATE AND TIME OF NECROPSY: 06/25/99 10:32 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 10 X 7 MM, TAN, RED, VASCULAR AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MATERIAL -ANIMAL THIN DRY RED HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT THYMUS (TH) : >SECTION EXAMINED; TISSUE NOT PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4270 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 105 ANIMAL NUMBER: C92228 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/25/99 STUDY DAY OF DEATH: 446 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 423.5 GRAMS DATE AND TIME OF NECROPSY: 06/25/99 10:32 PROSECTOR: JAMES T. CALLEN RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4271 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 106 ANIMAL NUMBER: C92229 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE; BILATERAL: EACH IS 33 X 20 X 17 MM LIVER (LI) : -MASS(ES); ATTACHED TO MARGIN OF LEFT LATERAL LOBE: SINGLE MASS, 30 X 25 X 15 MM, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (HEPATIC) NOT IDENTIFIED THYROID (TY) : -LARGE; BILATERAL: EACH IS 7 X 5 X 4 MM SKIN (SK) : -CRUSTED AREA(S); RIGHT DORSAL LUMBAR REGION: SINGLE TAN AREA, 3 X 2 MM (CAP 19) PITUITARY (PI) : -RAISED AREA(S); RIGHT POSTERIOR ASPECT: SINGLE RED AREA, 3 X 2 X 2 MM HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT THYROID (TY) : >UNREMARKABLE SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4272 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 107 ANIMAL NUMBER: C92229 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 20 X 15 X 13 MM, DARK RED, RED, TAN AND SOFT; CUT SURFACE EXUDES A LIGHT GREEN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED ILEUM (IL) : >AUTOLYTIC DEATH COMMENT (DC) : -HEPATOCELLULAR ADENOMA,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL 4273 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 108 ANIMAL NUMBER: C92229 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/26/99 STUDY DAY OF DEATH: 600 STUDY WEEK OF DEATH: 86 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/26/99 8:00 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4274 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 109 ANIMAL NUMBER: C92230 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 731.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0289 .0255 2.3457 2.6137 2.5839 27.4278 2.3634 .9887 1.8038 1.7845 .0214 .0196 .0040 % .0035 % .3209 % .3576 % .3535 % 3.7521 % .3233 % .1353 % .2468 % .2441 % .0029 % .0027 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0123 .0109 1.0000 1.1143 1.1015 11.6928 1.0075 .4215 .7690 .7608 .0091 .0084 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -MOTTLED; ALL LOBES: RED, BROWN AND TAN GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATELY SEVERE, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4275 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 110 ANIMAL NUMBER: C92230 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 731.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4276 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 111 ANIMAL NUMBER: C92231 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 801.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:01 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; VENTRALTHORACIC-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS SKIN (SK) : -CRUSTED AREA(S); VENTRAL THORACIC REGION: SINGLE RED, DRY CRUSTED AREA, 2 MM IN DIAMETER (CAP 22) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -HYPERPLASIA, ISLET CELLS,-MODERATE, FOCAL SKIN (SK) : -B-ADENOMA, BASAL CELL,-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY,-SLIGHT, FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HYPERPLASIA,-MODERATE, MULTI-FOCAL 4277 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 112 ANIMAL NUMBER: C92231 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 801.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:01 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PROSTATE (PR) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT MULTI 4278 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 113 ANIMAL NUMBER: C92231 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 801.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:01 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4279 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 114 ANIMAL NUMBER: C92232 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 624.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:54 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 10 X 10 MM BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -ROUGH SURFACE; DIFFUSE HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -HYPERTROPHY, VASCULAR,-MODERATE, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL TESTIS (TE) : -MINERALIZATION,-MINIMAL, FOCAL BRAIN (BR) : >UNREMARKABLE HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL SPLEEN (SP) : >UNREMARKABLE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SLIGHT, FOCAL 4280 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 115 ANIMAL NUMBER: C92232 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 624.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:54 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -CYST(S); SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 2 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERIANAL: MOIST BROWN MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -STAINS - SKIN/HAIRCOAT; INVOLVING ENTIRE HEAD: DRY RED MATERIAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MODERATE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL MULTI PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL 4281 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 116 ANIMAL NUMBER: C92232 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 624.7 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:54 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4282 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 117 ANIMAL NUMBER: C92233 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 373.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:40 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 8 X 9 X 18 MM BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 9 MM, FIRM, DARK RED AND TAN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL 4283 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 118 ANIMAL NUMBER: C92233 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 373.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:40 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 10 X 17 X 26 MM, FIRM, LOBULATED, TAN AND RED; ON SECTION SOME AREAS EXUDE TAN CREAMY SEMIFLUID (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST, YELLOW MATERIAL -ANIMAL THIN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 4284 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 119 ANIMAL NUMBER: C92233 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 373.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:40 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES HEMATO NEOPLASIA (HN) (JE), (EP), 4285 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 120 ANIMAL NUMBER: C92234 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 681.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 14:25 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 5 X 5 MM, SOFT STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE RED, TAN AND HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT THYMUS (TH) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL 4286 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 121 ANIMAL NUMBER: C92234 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 681.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 14:25 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN ERODED AREA, 4 X 2 MM FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DARK RED, MOIST ABRADED AREA, 16 X 12 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW AND BROWN MATERIAL STOMACH, NONGL (SU) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : -EROSION,-SEVERE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SEVERE, FOCAL 4287 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 122 ANIMAL NUMBER: C92234 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 681.0 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 14:25 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4288 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 123 ANIMAL NUMBER: C92235 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 601.3 GRAMS DATE AND TIME OF NECROPSY: 02/11/00 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; SWOLLEN; NOSE; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE DARK RED AREA, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION SKIN (SK) : -SUBCUTANEOUS EDEMA; SURROUNDING NARES: SMALL AMOUNT OF CLEAR AND GELATINOUS EDEMA (CAP 20) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -INFARCT,-MODERATE, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SKIN (SK) : -EDEMA,-MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT 4289 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 124 ANIMAL NUMBER: C92235 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 601.3 GRAMS DATE AND TIME OF NECROPSY: 02/11/00 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL (CAP 19) CALVARIUM/SKULL (CA) : -RED FOCUS(I)/AREA(S); ROSTRAL TO EYES: SINGLE DARK RED AREA, 15 X 10 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN, RED AND DRY MATERIAL -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL CALVARIUM/SKULL (CA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4290 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 125 ANIMAL NUMBER: C92235 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/11/00 STUDY DAY OF DEATH: 677 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 601.3 GRAMS DATE AND TIME OF NECROPSY: 02/11/00 14:48 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4291 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 126 ANIMAL NUMBER: C92236 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 8:42 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, DARK RED, GRAY, AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, MULTI-FOCAL EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-SLIGHT, MULTI-FOCAL -VASCULARIZATION, CORNEAL,-MODERATE, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL ILEUM (IL) : >AUTOLYTIC 4292 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 127 ANIMAL NUMBER: C92236 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 8:42 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -DISCHARGE-NASAL; DARK RED AND DRY PROSTATE (PR) : ^UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4293 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 128 ANIMAL NUMBER: C92236 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/20/99 STUDY DAY OF DEATH: 624 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/20/99 8:42 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN), MAMMARY, MALE (MM) 4294 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 129 ANIMAL NUMBER: C92237 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 607.6 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 15:43 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 8 X 6 MM, DARK RED, RED, TAN AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL 4295 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 130 ANIMAL NUMBER: C92237 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 607.6 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 15:43 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DRY BROWN CRUSTED AREA, 10 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: MOIST DARK BROWN MATERIAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : -INFLAMMATION, ACUTE,-MODERATE, FOCAL 4296 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 131 ANIMAL NUMBER: C92237 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/11/00 STUDY DAY OF DEATH: 706 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 607.6 GRAMS DATE AND TIME OF NECROPSY: 03/11/00 15:43 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4297 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 132 ANIMAL NUMBER: C92238 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 775.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:21 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0322 .0351 2.4208 2.4709 2.4580 20.9165 2.0432 1.0204 1.8447 1.8528 .0218 .0264 .0042 % .0045 % .3120 % .3185 % .3168 % 2.6958 % .2633 % .1315 % .2377 % .2388 % .0028 % .0034 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0133 .0145 1.0000 1.0207 1.0154 8.6403 .8440 .4215 .7620 .7654 .0090 .0109 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4298 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 133 ANIMAL NUMBER: C92238 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 775.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:21 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4299 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 134 ANIMAL NUMBER: C92239 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 474.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:17 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, GRAY, RED AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL 4300 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 135 ANIMAL NUMBER: C92239 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 474.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:17 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DARK RED, DRY CRUSTED AREA, 6 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; EXTENDING TO MID ABDOMINAL REGION: BROWN, DRY MATERIAL -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -FIBROSIS,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL LN, MEDIASTINAL (ML) : -DILATATION, SINUSOIDAL,-MODERATE, FOCAL >NOTE:>MEDIASTINAL LYMPH NODE PRESENT ON THYMUS SECTION 4301 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 136 ANIMAL NUMBER: C92239 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 474.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:17 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (SM), (JE), (EP) 4302 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 137 ANIMAL NUMBER: C92240 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/04/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/04/99 13:13 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED FOCI, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE DRY, DARK RED CRUSTED AREA, 13 X 8 MM; SURROUNDING TISSUE APPEARS SWOLLEN; COLLECTED HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT FOOT/FOOTPAD (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 4303 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 138 ANIMAL NUMBER: C92240 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/04/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/04/99 13:13 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 30 X 23 X 12 MM, RED, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 19) GENERAL COMMENT (GC) : -ANIMAL OBESE MAMMARY, MALE (MM) : >TISSUE MISSING >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS FIBROMA 4304 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 139 ANIMAL NUMBER: C92240 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 11/04/99 STUDY DAY OF DEATH: 578 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 11/04/99 13:13 PROSECTOR: CHANESTA ACKER RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) (PR), 4305 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 140 ANIMAL NUMBER: C92241 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 669.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND TAN HEART (HT) : -MASS(ES); TRIMMING COMMENT: WITHIN RIGHT ATRIUM: SINGLE MASS, 3 MM IN DIAMETER, TAN AND FIRM; SUBMITTED WITH ROUTINE SECTION PITUITARY (PI) : -RAISED AREA(S); LEFT ANTERIOR ASPECT: SINGLE TAN AREA, 3 X 2 X 2 MM HISTOPATHOLOGY KIDNEY (KD) : -INFARCT,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, DIFFUSE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE, CILIARY,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL -THROMBUS, ATRIAL,-MODERATELY SEVERE, FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4306 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 141 ANIMAL NUMBER: C92241 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 669.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED AND DRY PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -ATRIAL THROMBUS,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4307 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 142 ANIMAL NUMBER: C92241 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/10/00 STUDY DAY OF DEATH: 645 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 669.0 GRAMS DATE AND TIME OF NECROPSY: 01/10/00 12:59 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4308 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 143 ANIMAL NUMBER: C92242 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:30 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, DARK RED, TAN AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE DARK RED AND TAN ERODED AREAS, UP TO 16 X 7 MM PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: CLEAR HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -PYELONEPHRITIS,-SLIGHT, FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, DIFFUSE -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL -ULCERATION,-MODERATELY SEVERE, FOCAL PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT 4309 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 144 ANIMAL NUMBER: C92242 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:30 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST BROWN AND DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4310 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 145 ANIMAL NUMBER: C92242 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/14/99 STUDY DAY OF DEATH: 465 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 576.2 GRAMS DATE AND TIME OF NECROPSY: 07/14/99 15:30 PROSECTOR: WENDY CHERRY RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4311 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 146 ANIMAL NUMBER: C92243 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 485 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 611.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 11:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 9 X 8 MM, RED, DARK RED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; VENTRAL ABDOMINAL REGION: DRY RED MATERIAL HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY,-MODERATE, FOCAL -THROMBUS,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT MULTI 4312 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 147 ANIMAL NUMBER: C92243 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/03/99 STUDY DAY OF DEATH: 485 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 611.3 GRAMS DATE AND TIME OF NECROPSY: 08/03/99 11:30 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (SM), 4313 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 148 ANIMAL NUMBER: C92244 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 453 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 10:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HINDFOOT: SINGLE DARK RED AND DRY CRUSTED AREA, 2 X 1 MM; COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-NASAL; MOIST RED MATERIAL HISTOPATHOLOGY LUNG (LU) : -INFLAMMATION, CHRONIC,-MODERATELY SEVERE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT FOOT/FOOTPAD (FP) : -EROSION,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL 4314 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 149 ANIMAL NUMBER: C92244 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 07/02/99 STUDY DAY OF DEATH: 453 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 07/02/99 10:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) (FE), 4315 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 150 ANIMAL NUMBER: C92245 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 482.0 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 14:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DEPRESSED FOCUS(I)/AREA(S); VISCERAL SURFACE OF RIGHT MEDIAN LOBE: SINGLE TAN AND LINEAR AREA, 12 X 3 MM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL MISSING AT TIME OF TRIMMING ADRENAL, MEDULLA (MA) : >NOTE:>TRIMMING COMMENT: ONE ADRENAL MISSING AT TIME OF TRIMMING PITUITARY (PI) : -MASS(ES); 11 X 9 X 8 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, NEAR PYLORIC VALVE: SINGLE TAN AND SLIGHTLY ERODED AREA, 3 X 2 MM HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-MODERATE, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ONE EXAMINED,-PRESENT ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 4316 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 151 ANIMAL NUMBER: C92245 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 482.0 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 14:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER AND LOWER INCISORS -ANIMAL THIN -STAINS - SKIN/HAIRCOAT; DIFFUSE: YELLOW PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4317 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 152 ANIMAL NUMBER: C92245 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 482.0 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 14:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4318 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 153 ANIMAL NUMBER: C92246 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 674.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:56 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0316 .0328 2.3732 2.1678 1.9672 17.2687 2.1696 .9390 1.7567 1.7110 .0161 .0241 .0047 % .0049 % .3516 % .3212 % .2915 % 2.5587 % .3215 % .1391 % .2603 % .2535 % .0024 % .0036 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0133 .0138 1.0000 .9135 .8289 7.2765 .9142 .3957 .7402 .7210 .0068 .0102 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4319 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 154 ANIMAL NUMBER: C92246 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 674.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:56 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4320 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 155 ANIMAL NUMBER: C92247 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 884.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/99 8:38 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0326 .0416 2.7388 2.8337 2.7175 26.2003 2.3746 1.6381 1.9421 1.9533 .0380 .0382 .0037 % .0047 % .3095 % .3203 % .3071 % 2.9612 % .2684 % .1851 % .2195 % .2208 % .0043 % .0043 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0119 .0152 1.0000 1.0347 .9922 9.5663 .8670 .5981 .7091 .7132 .0139 .0139 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED PITUITARY (PI) : >NOTE:>TRIMMING COMMENT: NOT FOUND IN WET TISSUE AT TRIMMING GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, FOCAL PITUITARY (PI) : >TISSUE MISSING MULTI DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4321 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 156 ANIMAL NUMBER: C92247 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 884.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/99 8:38 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4322 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 157 ANIMAL NUMBER: C92248 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 444.3 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:55 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 15 X 9 X 8 MM, RED, TAN, SOFT AND LOBULATED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4323 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 158 ANIMAL NUMBER: C92248 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/26/99 STUDY DAY OF DEATH: 477 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 444.3 GRAMS DATE AND TIME OF NECROPSY: 07/26/99 13:55 PROSECTOR: JILL PAUS RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4324 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 159 ANIMAL NUMBER: C92249 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 499 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:24 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : >NOTE:>TRIMMING COMMENT: MISSING AT TIME OF TRIMMING PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 6 X 6 MM, RED, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 X 1 MM HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : >TISSUE MISSING PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, FOCAL -EROSION,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC CECUM (CE) : >AUTOLYTIC 4325 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 160 ANIMAL NUMBER: C92249 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/17/99 STUDY DAY OF DEATH: 499 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/17/99 14:24 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DARK RED AND TAN ABRADED AREA, 10 X 7 MM; COLLECTED DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4326 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 161 ANIMAL NUMBER: C92250 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 872.9 GRAMS DATE AND TIME OF NECROPSY: 01/02/00 13:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); POSTERIOR ASPECT: 10 X 7 X 7 MM, DARK RED AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HYPERTROPHY,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT EPIDIDYMIDES (EP) : -GRANULOMA, SPERM,-MODERATE, FOCAL 4327 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 162 ANIMAL NUMBER: C92250 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 872.9 GRAMS DATE AND TIME OF NECROPSY: 01/02/00 13:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE TAN AND MOIST ABRADED AREA, 3 MM IN DIAMETER; COLLECTED LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT, LUMBAR, INVOLVING ENTIRE NODE: SINGLE CLEAR FLUID FILLED CYST, 18 X 17 X 12 MM (LEFT IS 5 X 4 X 4 MM) (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; RIGHT: DARK RED AND DRY; LEFT EYE (RED DISCHARGE) NOT SEEN AT NECROPSY DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL 4328 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 163 ANIMAL NUMBER: C92250 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/02/00 STUDY DAY OF DEATH: 637 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: 872.9 GRAMS DATE AND TIME OF NECROPSY: 01/02/00 13:45 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), HEMATO NEOPLASIA (HN) 4329 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 164 ANIMAL NUMBER: C92251 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 750.7 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 78; 80 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; LATERALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN ADRENAL, CORTEX (AC) : -MISSING/NOT IDENTIFIED; RIGHT: NOT POSITIVELY IDENTIFIED; POSSIBLE REMNANTS COLLECTED HISTOPATHOLOGY LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ONE EXAMINED,-PRESENT 4330 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 165 ANIMAL NUMBER: C92251 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 750.7 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM MAMMARY, MALE (MM) : -MASS(ES); RIGHT VENTRAL ABDOMINAL REGION: SINGLE MASS, 95 X 75 X 46 MM, TAN, RED, DARK RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE DRY, DARK RED CRUSTED AREA, 38 X 25 MM; COLLECTED WITH MASS; ADHESIONS ASSOCIATED WITH MASS, PLEASE SEE NOTES (MM) ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT STOMACH, GL (ST) : ^UNREMARKABLE URINARY BLADDER (UB) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL DEATH COMMENT (DC) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT MAMMARY, MALE (MM) : >TISSUE MISSING >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS HISTIOCYTIC SARCOMA 4331 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 166 ANIMAL NUMBER: C92251 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 750.7 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : >NOTE:>PORTIONS OF MASS ADHERED TO ADIPOSE TISSUE SURROUNDING STOMACH, TESTES AND EPIDIDYMIDES: MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED INTACT WITH MASS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY 4332 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 167 ANIMAL NUMBER: C92251 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/08/99 STUDY DAY OF DEATH: 582 STUDY WEEK OF DEATH: 84 TERMINAL BODY WEIGHT: 750.7 GRAMS DATE AND TIME OF NECROPSY: 11/08/99 14:45 PROSECTOR: TONY HANFELD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), PITUITARY (PI), HARDERIAN GLAND SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP) (HG), 4333 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 168 ANIMAL NUMBER: C92252 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:51 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY THYROID (TY) : -LARGE; RIGHT: 7 X 5 X 4 MM (LEFT IS 6 X 4 X 3 MM) STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA, AT JUNCTION OF GLANDULAR AND NON GLANDULAR: SINGLE TAN ERODED AREA, 15 X 9 MM; SURROUNDING TISSUE APPEARS THICKENED HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL THYROID (TY) : -FIBROSIS,-SLIGHT, MULTI-FOCAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EROSION,-MODERATELY SEVERE, FOCAL 4334 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 169 ANIMAL NUMBER: C92252 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:51 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN STOMACH, NONGL (SU) : -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL -B-LEIOMYOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4335 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 170 ANIMAL NUMBER: C92252 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:51 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4336 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 171 ANIMAL NUMBER: C92253 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 753.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:25 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0239 .0294 2.2490 2.2509 2.1521 17.8062 2.0111 1.1340 2.0998 2.1700 .0184 .0187 .0032 % .0039 % .2985 % .2987 % .2856 % 2.3631 % .2669 % .1505 % .2787 % .2880 % .0024 % .0025 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0106 .0131 1.0000 1.0008 .9569 7.9174 .8942 .5042 .9337 .9649 .0082 .0083 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); NEAR MARGIN OF MEDIAN LOBES: SINGLE TAN AREA, 4 X 3 MM (CAP 18) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LIVER (LI) : -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE, FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT MULTI 4337 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 172 ANIMAL NUMBER: C92253 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 753.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:25 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), HEMATO NEOPLASIA (HN) 4338 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 173 ANIMAL NUMBER: C92254 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 443.1 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 13:18 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN TESTIS (TE) : -SMALL; BILATERAL: EACH IS 19 X 12 X 8 MM; BOTH ARE SOFT SKIN (SK) : -CRUSTED AREA(S); LEFT LATERAL ABDOMINAL REGION: SINGLE TAN, SLIGHTLY RAISED AREA, 5 MM IN DIAMETER; COLLECTED HISTOPATHOLOGY LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,-MINIMAL, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE -THROMBUS,-MODERATELY SEVERE, FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATE, MULTI FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT 4339 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 174 ANIMAL NUMBER: C92254 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 443.1 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 13:18 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RAISED AREA(S); RIGHT LATERAL ASPECT: SINGLE TAN AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA, NEAR CARDIAC SPHINCTER: SINGLE TAN, RAISED AREA, 1 MM IN DIAMETER STOMACH, NONGL (SU) : -THICKENED WALL; MUCOSA: MULTIPLE TAN THICKENED AREAS, UP TO 5 X 4 MM PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: TAN AND FIRM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 12 X 8 X 2 MM HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -DILATATION, SINUSOIDAL,-SLIGHT, FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL SEMINAL VESICLES (SV) : -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL 4340 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 175 ANIMAL NUMBER: C92254 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 443.1 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 13:18 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE TAN CRUSTED AREA, 9 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED -ANIMAL THIN EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT FOOT/FOOTPAD (FP) : -CYST, KERATIN,-PRESENT -FIBROSIS,-MODERATELY SEVERE, FOCAL -INFLAMMATION, CHRONIC ACTIVE,-MINIMAL MULTI-FOCAL 4341 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 176 ANIMAL NUMBER: C92254 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/29/99 STUDY DAY OF DEATH: 603 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 443.1 GRAMS DATE AND TIME OF NECROPSY: 11/29/99 13:18 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), HEMATO NEOPLASIA (HN) 4342 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 177 ANIMAL NUMBER: C92255 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 409.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:07 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 11.6239 ------------------------------------------- ------------------------------------ 2.8420 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4343 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 178 ANIMAL NUMBER: C92255 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 409.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:07 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4344 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 179 ANIMAL NUMBER: C92256 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 557.9 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:48 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 17.1282 ------------------------------------------- ------------------------------------ 3.0701 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4345 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 180 ANIMAL NUMBER: C92256 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 557.9 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:48 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4346 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 181 ANIMAL NUMBER: C92257 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:08 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 13.8818 ------------------------------------------- ------------------------------------ 2.8267 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT APPLICABLE GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4347 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 182 ANIMAL NUMBER: C92257 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.1 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:08 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4348 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 183 ANIMAL NUMBER: C92258 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 549.3 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:13 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 15.7545 ------------------------------------------- ------------------------------------ 2.8681 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4349 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 184 ANIMAL NUMBER: C92258 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 549.3 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:13 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4350 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 185 ANIMAL NUMBER: C92259 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 343.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:34 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 13.7164 ------------------------------------------- ------------------------------------ 3.9966 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4351 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 186 ANIMAL NUMBER: C92259 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 343.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:34 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4352 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 187 ANIMAL NUMBER: C92260 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:19 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 14.0868 ------------------------------------------- ------------------------------------ 2.8078 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4353 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 188 ANIMAL NUMBER: C92260 SEX: MALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 501.7 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:19 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4354 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 189 ANIMAL NUMBER: C92261 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 775.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:26 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4355 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 190 ANIMAL NUMBER: C92261 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 775.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:26 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE), URINARY BLADDER (UB) 4356 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 191 ANIMAL NUMBER: C92262 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 9:47 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; HEAD-ENTIRE; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ALOPECIA; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN -LARGE; BILATERAL: EACH IS 29 X 18 X 12 MM LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -MASS(ES); RIGHT MEDIAN AND RIGHT LATERAL LOBES: SINGLE MASS ON EACH, RIGHT MEDIAN IS 30 X 25 X 20 MM, RIGHT LATERAL IS 35 X 20 X 12 MM, BOTH ARE TAN, RED AND VASCULAR, BOTH ARE SAME ON CUT SURFACE; RIGHT MEDIAN COLLECTED ON ROUTINE SECTION, RIGHT LATERAL (CAP 24); REGIONAL LN (SEE HEPATIC) -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED HISTOPATHOLOGY LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATELY SEVERE, FOCAL -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFILTRATE, MAST CELLS,-SLIGHT, MULTI FOCAL -B-ADENOMA, HEPATOCELLULAR,- 2 NEOPLASMS PRESENT PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL 4357 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 192 ANIMAL NUMBER: C92262 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 9:47 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 1 MM IN DIAMETER TESTIS (TE) : -SMALL; BILATERAL: EACH IS 17 X 8 X 6 MM; BOTH ARE SOFT SKIN (SK) : -CRUSTED AREA(S); DORSAL HEAD AND DORSAL THORACIC REGION: MULTIPLE DARK RED, TAN, DRY CRUSTED AREAS, UP TO 5 MM IN DIAMETER (CAP 22) -ALOPECIA-DIFFUSE; (CAP 23) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF LEFT VENTRICLE: SINGLE GRAY AREA, 4 X 3 MM SPLEEN (SP) : -DEPRESSED FOCUS(I)/AREA(S); CRANIAL END: MULTIPLE LINEAR DEPRESSED AREAS, UP TO 6 X 1 MM ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 MM IN DIAMETER -DIFFUSELY LIGHT; BILATERAL: TAN PARATHYROID (PT) : >UNREMARKABLE TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE, DIFFUSE SKIN (SK) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, FOCAL MULTI SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MODERATE, FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL 4358 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 193 ANIMAL NUMBER: C92262 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 9:47 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 4 MM IN DIAMETER, DARK RED AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM -THICKENED WALL; DIFFUSE PROSTATE (PR) : -SMALL; 15 X 10 X 4 MM SEMINAL VESICLES (SV) : -SMALL; BILATERAL: EACH IS 10 X 7 X 2 MM EPIDIDYMIDES (EP) : -SMALL; BILATERAL: EACH IS 43 X 5 X 4 MM LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 4 X 2 X 2 MM, RIGHT IS 6 X 5 X 2 MM (CAP 26) -DIFFUSELY RED; BILATERAL: DARK RED LN, OTHER (LN) : -LARGE; HEPATIC: UP TO 5 X 3 X 2 MM (CAP 25) ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -MINERALIZATION,-MODERATE, MULTI-FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : >UNREMARKABLE EPIDIDYMIDES (EP) : -HYPOSPERMIA,-PRESENT DEATH COMMENT (DC) : -HEPATOCELLULAR ADENOMA,-PRESENT LN, RENAL (RL) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL LN, OTHER (LN) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL 4359 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 194 ANIMAL NUMBER: C92262 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 458.8 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 9:47 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MEs /pANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), LN, MESENTERIC (MS) 4360 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 195 ANIMAL NUMBER: C92263 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 752.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:11 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); TAIL-PROXIMAL; WEEK 64; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY PANCREAS (PA) : -MASS(ES); DUODENUM REGION: SINGLE MASS, 15 X 10 X 10 MM, RED, DARK RED, TAN, AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (PANCREATIC) NOT IDENTIFIED SPLEEN (SP) : -RAISED FOCUS(I)/AREA(S); PARIETAL SURFACE, ALONG MARGIN: SINGLE RED AREA, 7 X 5 X 4 MM HISTOPATHOLOGY KIDNEY (KD) : -CYST,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT SPLEEN (SP) : -B-LEIOMYOMA,-NEOPLASM PRESENT 4361 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 196 ANIMAL NUMBER: C92263 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 752.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:11 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TAIL (TI) : -RAISED AREA(S); NEAR BASE: SINGLE DRY, TAN RAISED AREA, 8 X 2 X 1 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE), URINARY BLADDER (UB) 4362 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 197 ANIMAL NUMBER: C92264 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 313.7 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:34 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 11.7274 ------------------------------------------- ------------------------------------ 3.7384 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4363 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 198 ANIMAL NUMBER: C92264 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 313.7 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:34 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4364 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 199 ANIMAL NUMBER: C92265 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 559 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/16/99 8:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY LUNG (LU) : -UNCOLLAPSED; ALL LOBES KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN -LARGE; BILATERAL: EACH IS 30 X 25 X 21 MM LIVER (LI) : -RED FOCUS(I)/AREA(S); VISCERAL SURFACE OF RIGHT MEDIAN LOBE: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTION PANCREAS (PA) : -LARGE VESSELS; DIFFUSE, ALONG DUODENUM: UP TO 4 MM IN DIAMETER (CAP 21) PARATHYROID (PT) : -LARGE; LEFT: 3 X 2 X 1 MM (RIGHT IS 1 MM IN DIAMETER) EYE (EY) : -OPAQUE CORNEA; RIGHT HISTOPATHOLOGY LUNG (LU) : ^UNREMARKABLE KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE LIVER (LI) : >UNREMARKABLE PANCREAS (PA) : -THROMBUS,-SEVERE, MULTI-FOCAL PARATHYROID (PT) : -HYPERPLASIA,-MODERATELY SEVERE, DIFFUSE EYE (EY) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATE, FOCAL 4365 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 200 ANIMAL NUMBER: C92265 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 559 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/16/99 8:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); TISSUE SURROUNDING MID ASPECT: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK FOCI, UP TO 4 MM IN DIAMETER PROSTATE (PR) : -SMALL; 10 X 8 X 7 MM AORTA (AO) : -LARGE; DIFFUSE: UP TO 6 MM IN DIAMETER -FIRM; DIFFUSE LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 6 X 5 X 5 MM, RIGHT IS 4 X 4 X 3 MM -DIFFUSELY RED; BILATERAL: DARK RED (CAP 19) LN, LUMBAR/ILIAC (LM) : -DIFFUSELY DARK; BILATERAL: BLACK (CAP 20) GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, LOWER INCISORS PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT AORTA (AO) : -MINERALIZATION,-MINIMAL, MULTI-FOCAL LN, RENAL (RL) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL LN, LUMBAR/ILIAC (LM) : >UNREMARKABLE 4366 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 201 ANIMAL NUMBER: C92265 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/16/99 STUDY DAY OF DEATH: 559 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/16/99 8:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN OFF AT BASE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY) 4367 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 202 ANIMAL NUMBER: C92266 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 805.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 76; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); VENTRAL ABDOMINAL; WEEK 92; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL LOBE: SINGLE TAN AREA, 2 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION SKIN (SK) : -CRUSTED AREA(S); MID DORSAL LUMBAR REGION: SINGLE DARK RED AND DRY AREA, 21 X 22 MM; COLLECTED -RAISED AREA(S); MID ABDOMINAL REGION: SINGLE TAN AND RED AREA, 6 X 5 X 5 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT -EROSION,-SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL -B-FIBROMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4368 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 203 ANIMAL NUMBER: C92266 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 805.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:54 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4369 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 204 ANIMAL NUMBER: C92267 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 611.7 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:16 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0250 .0313 2.4148 2.1305 2.1979 16.2986 2.0076 1.0302 1.9753 2.0027 .0232 .0296 .0041 % .0051 % .3948 % .3483 % .3593 % 2.6645 % .3282 % .1684 % .3229 % .3274 % .0038 % .0048 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0104 .0130 1.0000 .8823 .9102 6.7495 .8314 .4266 .8180 .8293 .0096 .0123 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4370 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 205 ANIMAL NUMBER: C92267 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 611.7 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:16 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4371 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 206 ANIMAL NUMBER: C92268 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 675.4 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; SCROTUMRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -GRANULAR MATERIAL; BILATERAL, WITHIN PELVIS: MULTIPLE TAN PINPOINT GRANULES SKIN (SK) : -CRUSTED AREA(S); OVERLYING RIGHT SCROTAL SAC: SINGLE TAN CRUSTED AREA, 6 MM IN DIAMETER; COLLECTED (CAP 19) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 5 X 5 MM (RIGHT IS 5 X 3 X 3 MM) PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, RED, DARK RED, TAN, AND FIRM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO BROWN AREAS, UP TO 4 X 2 MM HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATELY SEVERE, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT SKIN (SK) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL BRAIN (BR) : >UNREMARKABLE ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 4372 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 207 ANIMAL NUMBER: C92268 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 675.4 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:05 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY URINARY BLADDER (UB) : -DIFFUSELY RED; MUCOSA GENERAL COMMENT (GC) : -BLOOD FILM TAKEN URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE) 4373 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 208 ANIMAL NUMBER: C92269 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/24/99 11:14 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE GENERAL COMMENT (GC) : -ANIMAL OBESE HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL STOMACH, GL (ST) : >UNREMARKABLE DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT 4374 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 209 ANIMAL NUMBER: C92269 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/24/99 STUDY DAY OF DEATH: 506 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/24/99 11:14 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (EP), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), TESTIS (TE) 4375 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 210 ANIMAL NUMBER: C92270 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 757.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; BILATERAL: EACH IS 20 X 14 X 5 MM; BOTH APPEAR SOFT PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 4 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE -MINERALIZATION,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4376 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 211 ANIMAL NUMBER: C92270 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 757.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:06 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY) 4377 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 212 ANIMAL NUMBER: C92271 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 625 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 8:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 90; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 5 MM IN DIAMETER -ADHESION(S); ALL LOBES TO DIAPHRAGM: MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED INTACT KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS THYROID (TY) : -LARGE; BILATERAL: EACH IS 10 X 5 X 5 MM HISTOPATHOLOGY LUNG (LU) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, DIFFUSE LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : >UNREMARKABLE SKIN (SK) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 4378 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 213 ANIMAL NUMBER: C92271 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 625 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 8:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SPLEEN (SP) : -LIGHT FOCUS(I)/AREA(S); PARIETAL SURFACE: SINGLE YELLOW AREA, 4 X 3 MM ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 8 MM (RIGHT IS 7 X 6 X 6 MM) STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN AREAS, UP TO 4 X 3 MM -ADHESION(S); SEROSA ADHERED TO LEFT LATERAL LOBE OF LIVER: MULTIPLE TAN FIBROUS ADHESIONS; COLLECTED INTACT WITH STOMACH MAMMARY, MALE (MM) : -MASS(ES); DORSAL LUMBAR REGION: SINGLE MASS, 20 X 15 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST SPLEEN (SP) : >UNREMARKABLE ADRENAL, CORTEX (AC) : -VACUOLATION,-MODERATE, FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DEATH COMMENT (DC) : -HISTIOCYTIC SARCOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT MAMMARY, MALE (MM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 4379 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 214 ANIMAL NUMBER: C92271 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/21/99 STUDY DAY OF DEATH: 625 STUDY WEEK OF DEATH: 90 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/21/99 8:32 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; 30 X 25 X 20 MM -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST -DISCHARGE-NASAL; DARK RED AND MOIST THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: PANCREAS (PA), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4380 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 215 ANIMAL NUMBER: C92272 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 671.7 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 10:10 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK 74; 30 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; HEAD MAXILLARY-RIGHT; VERIFIED AT NECROPSY OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : ^UNREMARKABLE SKIN (SK) : -EROSION,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MUSCLE, SKELETAL (SM) : -I-SQUAMOUS CELL CARCINOMA,-NEOPLASM PRESENT 4381 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 216 ANIMAL NUMBER: C92272 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 671.7 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 10:10 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MUSCLE, SKELETAL (SM) : -MASS(ES); ATTACHED TO RIGHT MANDIBLE, SURROUNDING RIGHT PINNA: SINGLE MASS, 40 X 25 X 20 MM, RED, DARK RED, DRY, AND CRUSTED; ON SECTION, EXUDES A GREEN AND TAN CASEOUS MATERIAL; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 19); OVERLYING SKIN OF MASS HAS A SINGLE DARK RED CRUSTED AREA, 20 X 15 X 7 MM; COLLECTED INTACT WITH MASS FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE DARK RED, MOIST ABRADED AREA, 7 X 6 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN DEATH COMMENT (DC) : -SQUAMOUS CELL CARCINOMA,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, FOCAL -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL FOOT/FOOTPAD (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 4382 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 217 ANIMAL NUMBER: C92272 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/16/99 STUDY DAY OF DEATH: 590 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 671.7 GRAMS DATE AND TIME OF NECROPSY: 11/16/99 10:10 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE), MAMMARY, MALE (MM) 4383 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 218 ANIMAL NUMBER: C92273 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/24/99 STUDY DAY OF DEATH: 294 STUDY WEEK OF DEATH: 42 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/24/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY CECUM (CE) : -THICKENED WALL; MID CECUM GENERAL COMMENT (GC) : -ANIMAL OBESE HISTOPATHOLOGY KIDNEY (KD) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL CECUM (CE) : >UNREMARKABLE DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT MULTI 4384 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 219 ANIMAL NUMBER: C92273 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/24/99 STUDY DAY OF DEATH: 294 STUDY WEEK OF DEATH: 42 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/24/99 13:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4385 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 220 ANIMAL NUMBER: C92274 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 655.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:02 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0376 .0272 2.4537 2.2200 2.2397 21.2986 2.0321 1.1591 2.2539 2.2901 .0236 .0289 .0057 % .0041 % .3741 % .3385 % .3415 % 3.2472 % .3098 % .1767 % .3436 % .3492 % .0036 % .0044 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0153 .0111 1.0000 .9048 .9128 8.6802 .8282 .4724 .9186 .9333 .0096 .0118 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4386 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 221 ANIMAL NUMBER: C92274 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 655.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:02 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), TESTIS (TE) 4387 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 222 ANIMAL NUMBER: C92275 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 417.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LUNG (LU) : -ADHESION(S); ALL LOBES TO EACH OTHER AND PLEURA: MULTIPLE GREY AND FIBROUS ADHESIONS; COLLECTED INTACT -DIFFUSELY RED; ALL LOBES: DARK RED KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -CYST(S); BILATERAL, CORTEX EXTENDING INTO MEDULLA: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER PANCREAS (PA) : -LARGE VESSELS; DIFFUSE: UP TO 3 MM IN DIAMETER (CAP 22) PARATHYROID (PT) : -LARGE; BILATERAL: EACH IS 1 MM IN DIAMETER HISTOPATHOLOGY LUNG (LU) : -FIBROSIS, PLEURAL/SUBPLEURAL,MODERATE, MULTI-FOCAL KIDNEY (KD) : -CYST,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL -HYPERTROPHY, VASCULAR,-MODERATELY SEVERE, MULTI-FOCAL -INFLAMMATION, VASCULAR,-SLIGHT, MULTI FOCAL PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE 4388 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 223 ANIMAL NUMBER: C92275 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 417.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -LARGE; PROXIMAL TO CECUM: UP TO 9 X 5 X 5 MM (CAP 24) SPLEEN (SP) : -MASS(ES); CRANIAL END: SINGLE MASS, 9 MM IN DIAMETER, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (PANCREATIC) NOT IDENTIFIED PITUITARY (PI) : -MASS(ES); 8 X 6 X 5 MM, RED, DARK RED, TAN AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL TO URETHRA: TAN, GREEN AND FIRM LN, ANT MES/PANC (AP) : -LARGE; ANTERIOR MESENTERIC: UP TO 5 X 3 X 2 MM (CAP 23) TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : >UNREMARKABLE SPLEEN (SP) : -THROMBUS,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, ANT MES/PANC (AP) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL 4389 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 224 ANIMAL NUMBER: C92275 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 417.5 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 15:29 PROSECTOR: JERALD W. JOHNSON RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), THYROID (TY), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY) 4390 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 225 ANIMAL NUMBER: C92276 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/28/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/28/99 7:53 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; DORSALCERVICAL-RIGHT; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -UNCOLLAPSED; ALL LOBES KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY BROWN MATERIAL -ANIMAL THIN -DISCHARGE-ORAL; DRY BROWN MATERIAL HISTOPATHOLOGY LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -MINERALIZATION,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE TESTIS (TE) : -ATROPHY/DEGENERATION,-MODERATELY SEVERE, DIFFUSE DEATH COMMENT (DC) : -CHRONIC PROGRESSIVE NEPHROPATHY,PRESENT 4391 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 226 ANIMAL NUMBER: C92276 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/28/99 STUDY DAY OF DEATH: 510 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/28/99 7:53 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY) 4392 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 227 ANIMAL NUMBER: C92277 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/25/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 595.0 GRAMS DATE AND TIME OF NECROPSY: 05/25/99 13:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK RED, GREY, TAN AND SOFT STOMACH, GL (ST) : -LIGHT FOCUS(I)/AREA(S); MUCOSA: MULTIPLE GREY AREAS, UP TO 4 X 3 MM STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, NEAR CARDIAC VALVE: SINGLE DARK RED ERODED AREA, 5 X 4 X 3 MM; SURROUNDING TISSUE APPEARS SWOLLEN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MODERATE, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, DIFFUSE -EROSION,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4393 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 228 ANIMAL NUMBER: C92277 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/25/99 STUDY DAY OF DEATH: 415 STUDY WEEK OF DEATH: 60 TERMINAL BODY WEIGHT: 595.0 GRAMS DATE AND TIME OF NECROPSY: 05/25/99 13:14 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI) 4394 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 229 ANIMAL NUMBER: C92278 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 793.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:03 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED -LARGE; UP TO 7 MM IN DIAMETER SPLEEN (SP) : -RAISED FOCUS(I)/AREA(S); PARIETAL SURFACE: SINGLE DARK RED, TAN RAISED AREA, 8 X 7 X 7 MM PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: SINGLE RED AREA, 3 X 2 MM HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -N-HEMANGIOSARCOMA,-NEOPLASM PRESENT SPLEEN (SP) : -M-HEMANGIOSARCOMA,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4395 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 230 ANIMAL NUMBER: C92278 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 793.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:03 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) 4396 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 231 ANIMAL NUMBER: C92279 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 539.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 16:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); LEFT BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 10 X 8 MM, DARK RED, GREY AND SOFT PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM EPIDIDYMIDES (EP) : -MASS(ES); ATTACHED TO CAUDAL END OF RIGHT: SINGLE GREEN AND SOFT MASS, 8 X 5 X 5 MM; ON SECTION EXUDES A TAN CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 6 X 5 X 2 MM X 4 X 2 MM) (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN (LEFT IS 5 HISTOPATHOLOGY KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL EPIDIDYMIDES (EP) : -GRANULOMA, SPERM,-SEVERE, FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-SLIGHT, FOCAL MULTI 4397 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 232 ANIMAL NUMBER: C92279 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/13/99 STUDY DAY OF DEATH: 617 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 539.1 GRAMS DATE AND TIME OF NECROPSY: 12/13/99 16:00 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), SKIN (SK), MAMMARY, MALE (MM) 4398 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 233 ANIMAL NUMBER: C92280 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 306.1 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:29 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 12.3496 ------------------------------------------- ------------------------------------ 4.0345 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4399 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 234 ANIMAL NUMBER: C92280 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 306.1 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:29 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4400 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 235 ANIMAL NUMBER: C92281 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:39 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED FOCUS, 3 MM IN DIAMETER STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE BROWN AREA, LESS THAN 1 MM IN DIAMETER HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -INFARCT,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE, MULTI-FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4401 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 236 ANIMAL NUMBER: C92281 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 671.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:39 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) 4402 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 237 ANIMAL NUMBER: C92282 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 568.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:39 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >WART LIKE LESION(S); LIMB-FRONT-RIGHT; WEEK 105; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -CRUSTED AREA(S); RIGHT FRONT SHOULDER: SINGLE TAN RAISED AREA, 4 X 3 X 2 MM (CAP 22) LN, MESENTERIC (MS) : -DIFFUSELY RED STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: SINGLE TAN ERODED AREA, 3 MM IN DIAMETER; SURROUNDING TISSUE APPEARS SWOLLEN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL SKIN (SK) : -B-ADENOMA, BASAL CELL,-NEOPLASM PRESENT LN, MESENTERIC (MS) : >UNREMARKABLE STOMACH, NONGL (SU) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4403 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 238 ANIMAL NUMBER: C92282 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 568.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:39 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4404 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 239 ANIMAL NUMBER: C92283 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:04 PROSECTOR: TONY HANFELD RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); TAIL-MID; WEEK 101; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE TAIL (TI) : -RAISED AREA(S); 80 MM FROM TIP: SINGLE TAN RAISED AREA, 4 X 3 X 2 MM; COLLECTED LN, ANT MES/PANC (AP) : -LARGE; UP TO 6 X 4 X 4 MM (CAP 22) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, -INFARCT,-MINIMAL, FOCAL LIVER (LI) : >UNREMARKABLE FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : -CYST, EPIDERMAL INCLUSION,-PRESENT LN, ANT MES/PANC (AP) : >TISSUE MISSING >NOTE:>PANCREATIC LYMPH NODE DESCRIBED GROSSLY WAS A ISLET CELL ADENOMA OF THE PANCREAS 4405 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 240 ANIMAL NUMBER: C92283 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 683.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:04 PROSECTOR: TONY HANFELD RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), TESTIS (TE) 4406 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 241 ANIMAL NUMBER: C92284 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 11.1979 ------------------------------------------- ------------------------------------ 3.4993 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4407 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 242 ANIMAL NUMBER: C92284 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 320.0 GRAMS DATE AND TIME OF NECROPSY: 04/29/98 8:30 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4408 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 243 ANIMAL NUMBER: C92285 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 671.2 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 11:53 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HEART (HT) : -LIGHT FOCUS(I)/AREA(S); EPICARDIUM OF BOTH VENTRICLES: MULTIPLE INDISTINCT GREY AREAS PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, RED, DARK RED, TAN AND SOFT PROSTATE (PR) : -LARGE; 40 X 25 X 10 MM SEMINAL VESICLES (SV) : -LARGE; RIGHT: 38 X 20 X 8 MM (LEFT IS 25 X 15 X 4 MM) LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT: 13 X 6 X 5 MM (RIGHT IS 5 X 3 X 2 MM); BOTH APPEAR CYSTIC (CAP 19) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL 4409 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 244 ANIMAL NUMBER: C92285 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/14/99 STUDY DAY OF DEATH: 527 STUDY WEEK OF DEATH: 76 TERMINAL BODY WEIGHT: 671.2 GRAMS DATE AND TIME OF NECROPSY: 09/14/99 11:53 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4410 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 245 ANIMAL NUMBER: C92286 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:02 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0266 .0234 2.2692 2.5875 2.5622 21.6521 1.8660 1.0195 .7475 1.9291 .0175 .0214 .0035 % .0030 % .2943 % .3356 % .3323 % 2.8083 % .2420 % .1322 % .0970 % .2502 % .0023 % .0028 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0117 .0103 1.0000 1.1403 1.1291 9.5417 .8223 .4493 .3294 .8501 .0077 .0094 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI FOCAL 4411 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 246 ANIMAL NUMBER: C92286 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 771.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:02 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY TESTIS (TE) : -SMALL; LEFT: 2 1 X 1 0 X 4 MM (FOR COMPARISON RIGHT IS 26 X 12 X 7 MM) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE >NOTE:>LEFT TESTIS DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA) 4412 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 247 ANIMAL NUMBER: C92287 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:16 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0348 .0259 2.2991 1.9967 1.9629 22.1931 2.0899 1.0159 1.5601 1.5601 .0259 .0265 .0048 % .0036 % .3194 % .2774 % .2727 % 3.0828 % .2903 % .1411 % .2167 % .2167 % .0036 % .0037 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0151 .0113 1.0000 .8685 .8538 9.6530 .9090 .4419 .6786 .6786 .0113 .0115 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) -ANIMAL OBESE LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4413 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 248 ANIMAL NUMBER: C92287 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 719.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:16 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) 4414 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 249 ANIMAL NUMBER: C92288 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 853.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:03 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0402 .0302 2.4494 2.1246 2.2139 20.9207 1.6636 1.0951 1.7259 1.8021 .0253 .0203 .0047 % .0035 % .2871 % .2490 % .2595 % 2.4520 % .1950 % .1284 % .2023 % .2112 % .0030 % .0024 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0164 .0123 1.0000 .8674 .9039 8.5412 .6792 .4471 .7046 .7357 .0103 .0083 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT MULTI 4415 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 250 ANIMAL NUMBER: C92288 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 853.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:03 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4416 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 251 ANIMAL NUMBER: C92289 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 525.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:41 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN -LARGE; BILATERAL: EACH IS 14 X 18 X 28 MM LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS 2 X 5 X 14 MM, RIGHT IS 3 X 4 X 10 MM (CAP 22) -MOTTLED; BILATERAL: RED, DARK RED AND TAN LN, RENAL (RL) : -LARGE; BILATERAL: LEFT IS 5 X 5 X 8 MM, RIGHT IS 6 X 7 X 14 MM (CAP 23) -DIFFUSELY RED; BILATERAL: DARK RED HISTOPATHOLOGY KIDNEY (KD) : -CYST,-SLIGHT, MULTI-FOCAL -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, VACUOLATED,SLIGHT, MULTI-FOCAL LN, RENAL (RL) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 4417 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 252 ANIMAL NUMBER: C92289 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 525.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:41 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; -ANIMAL THIN -BONE MARROW SMEAR TAKEN BROWN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) 4418 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 253 ANIMAL NUMBER: C92290 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 360.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:57 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 9 X 7 MM, RED, RED, TAN, LOBULATED AND FIRM DARK GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4419 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 254 ANIMAL NUMBER: C92290 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 360.2 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:57 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4420 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 255 ANIMAL NUMBER: C92291 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE TAN AREA, 4 X 1 MM; COLLECTED ON ROUTINE SECTION ADRENAL, CORTEX (AC) : -LARGE; LEFT: 18 MM IN DIAMETER (RIGHT IS 3 MM IN DIAMETER) HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, DIFFUSE LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MODERATE, FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : >UNREMARKABLE >NOTE:>ADRENAL CORTICAL LESIONS DESCRIBED GROSSLY WERE A PHEOCHROMOCYTOMA OF THE MEDULLA ADRENAL, MEDULLA (MA) : -HEMATOPOIESIS, EXTRAMEDULLARY,MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 4421 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 256 ANIMAL NUMBER: C92291 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/07/99 8:20 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM BILE DUCT (BD) : -LARGE; UP TO 3 MM IN DIAMETER (CAP 19) DEATH COMMENT (DC) : -PHEOCHROMOCYTOMA,-PRESENT BILE DUCT (BD) : -DILATATION,-SEVERE, FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) 4422 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 257 ANIMAL NUMBER: C92292 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 373.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:22 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 9 X 9 MM, RED, TAN, GRAY, AND SOFT RED, DARK STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HIND FOOT: SINGLE TAN, MOIST, AND ABRADED AREA, 4 MM IN DIAMETER; COLLECTED TAIL (TI) : -CRUSTED AREA(S); PROXIMAL ONE-THIRD OF TAIL: MULTIPLE, TAN, AND DRY CRUSTED AREAS, UP TO 2 X 1 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN HISTOPATHOLOGY BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL TAIL (TI) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 4423 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 258 ANIMAL NUMBER: C92292 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/12/99 STUDY DAY OF DEATH: 463 STUDY WEEK OF DEATH: 67 TERMINAL BODY WEIGHT: 373.4 GRAMS DATE AND TIME OF NECROPSY: 07/12/99 14:22 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4424 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 259 ANIMAL NUMBER: C92293 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/18/99 STUDY DAY OF DEATH: 288 STUDY WEEK OF DEATH: 42 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/18/99 12:52 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -ROUGH SURFACE, DIFFUSE; BILATERAL URINARY BLADDER (UB) : -DIFFUSELY RED; DIFFUSE SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: YELLOW GENERAL COMMENT (GC) : -URINARY BLADDER-LARGE LUMEN; X 20 MM 35 X 25 HISTOPATHOLOGY KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, TUBULAR,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT URINARY BLADDER (UB) : >UNREMARKABLE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT DEATH COMMENT (DC) : -MALIGNANT LYMPHOMA,-PRESENT HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT 4425 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 260 ANIMAL NUMBER: C92293 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/18/99 STUDY DAY OF DEATH: 288 STUDY WEEK OF DEATH: 42 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/18/99 12:52 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), PROSTATE (PR), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE) 4426 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 261 ANIMAL NUMBER: C92294 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 378.8 GRAMS DATE AND TIME OF NECROPSY: 02/07/00 11:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); BILATERAL, CORTICAL SURFACE: SINGLE DARK BROWN AREA ON EACH, EACH IS 2 MM IN DIAMETER PITUITARY (PI) : -MASS(ES); 13 X 11 X 11 MM, RED, DARK RED, GRAY, TAN AND SOFT HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -HYPERTROPHY,-MODERATE, FOCAL PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT MULTI 4427 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 262 ANIMAL NUMBER: C92294 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/07/00 STUDY DAY OF DEATH: 673 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 378.8 GRAMS DATE AND TIME OF NECROPSY: 02/07/00 11:40 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); LEFT HIND FOOT: SINGLE TAN AND DRY CRUSTED AREA, 4 X 2 MM (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : ^UNREMARKABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (ST), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), TESTIS (TE) 4428 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 263 ANIMAL NUMBER: C92295 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 823.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:11 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; OPAQUE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY TESTIS (TE) : -SMALL; LEFT: 19 X 9 X 9 MM (RIGHT IS 18 X 10 X 10 MM); LEFT APPEARS SOFT EYE (EY) : -WHITE INTRAOCULAR MATERIAL; PITUITARY (PI) : -DIFFUSELY RED; DARK RED BILATERAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, DIFFUSE -MINERALIZATION,-MINIMAL, MULTI-FOCAL >NOTE:>LEFT TESTIS EYE (EY) : -CATARACT,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4429 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 264 ANIMAL NUMBER: C92295 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 823.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:11 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) 4430 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 265 ANIMAL NUMBER: C92296 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/12/99 STUDY DAY OF DEATH: 525 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 394.7 GRAMS DATE AND TIME OF NECROPSY: 09/12/99 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; UNVERIFIED AT NECROPSY >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; UNVERIFIED AT NECROPSY >EXCRETION; NO FECES; UNVERIFIED AT NECROPSY >BEHAVIOR; HYPOACTIVE; UNVERIFIED AT NECROPSY >EYES; RED DISCHARGE; EYES; UNVERIFIED AT NECROPSY OBSERVATIONS NECROPSY KIDNEY (KD) : -ROUGH SURFACE, DIFFUSE; BILATERAL -DIFFUSELY LIGHT; BILATERAL: TAN LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE TAN AREAS, UP TO 15 X 2 MM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 X 5 X 5 MM, DARK RED, RED, TAN, LOBULATED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST MATERIAL YELLOW, HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4431 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 266 ANIMAL NUMBER: C92296 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/12/99 STUDY DAY OF DEATH: 525 STUDY WEEK OF DEATH: 75 TERMINAL BODY WEIGHT: 394.7 GRAMS DATE AND TIME OF NECROPSY: 09/12/99 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED DRY MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), TESTIS (TE) 4432 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 267 ANIMAL NUMBER: C92297 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 640.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; LIMB-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 7 X 5 MM, DARK RED, TAN AND SOFT FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DARK RED, TAN AND MOIST ABRADED AREA, 6 X 5 X 4 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT >NOTE:>ONLY THE CALCULUS WAS PRESENT ON THE SECTION DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, CHRONIC ACTIVE,-MINIMAL, MULTI-FOCAL 4433 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 268 ANIMAL NUMBER: C92297 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 640.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:43 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4434 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 269 ANIMAL NUMBER: C92298 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 477.6 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 13:12 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL LOBE: SINGLE TAN AREA, 2 X 1 MM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 10 X 7 MM, DARK RED, TAN, LOBULATED, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 2 X 1 MM MAMMARY, MALE (MM) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, DIFFUSE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT MAMMARY, MALE (MM) : >TISSUE MISSING 4435 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 270 ANIMAL NUMBER: C92298 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 477.6 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 13:12 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST -ANIMAL THIN PERINEAL: THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4436 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 271 ANIMAL NUMBER: C92299 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 574.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:56 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DARK FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE BROWN AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, DARK RED, TAN AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 3 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL LIVER (LI) : >UNREMARKABLE MULTI PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-SLIGHT, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -FIBROSIS,-MODERATE, FOCAL 4437 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 272 ANIMAL NUMBER: C92299 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 574.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:56 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE DARK RED, TAN ABRADED AREA, 11 X 10 X 5 MM; COLLECTED >NOTE:>LEFT HINDFOOT SORE/SCAB NOT SEEN AS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), TESTIS (TE) 4438 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 273 ANIMAL NUMBER: C92300 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 10.1638 ------------------------------------------- ------------------------------------ 3.1942 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4439 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 274 ANIMAL NUMBER: C92300 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 318.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:46 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4440 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 275 ANIMAL NUMBER: C92301 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/01/99 8:01 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PROSTATE (PR) : -NODULAR; PROXIMAL SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY KIDNEY (KD) : -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MINIMAL, FOCAL PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT CAVITY, ABDOM (PC) : >TISSUE MISSING >NOTE:>MASS DESCRIBED GROSSLY WAS A PANCREATIC ISLET CELL ADENOMA 4441 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 276 ANIMAL NUMBER: C92301 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/01/99 8:01 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY CAVITY, ABDOM (PC) : -MASS(ES); WITHIN ADIPOSE OF ABDOMINAL CAVITY, NEAR MESENTERIC LYMPH NODES, NEAR COLON: SINGLE MASS, 8 X 8 X 7 MM, RED, DARK RED, TAN, FIRM AND NODULAR; CUT SURFACE EXUDES A RED FLUID (CAP 19); REGIONAL LN (MESENTERIC) NORMAL GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; YELLOW -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED 4442 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 277 ANIMAL NUMBER: C92301 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/01/99 8:01 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), SKIN (SK), LN, MESENTERIC (MS), MAMMARY, MALE (MM) 4443 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 278 ANIMAL NUMBER: C92302 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/02/00 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 712.5 GRAMS DATE AND TIME OF NECROPSY: 02/02/00 8:02 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; RECUMBENT; STERNAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT MEDIAN LOBE: SINGLE TAN AREA, 8 X 5 MM; COLLECTED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 8 MM, RED, DARK RED, TAN, SOFT STOMACH, GL (ST) : -EROSION/ULCERATION; MUCOSA: MULTIPLE BROWN ERODED AREAS, UP TO 5 X 2 MM PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREEN AND FIRM HISTOPATHOLOGY KIDNEY (KD) : -INFARCT,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -LIPIDOSIS, SUBCAPSULAR (TENSION LIPIDOSIS),-MODERATELY SEVERE, FOCAL -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : >UNREMARKABLE SEMINAL VESICLES (SV) : -SECRETION, INCREASED,-PRESENT 4444 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 279 ANIMAL NUMBER: C92302 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/02/00 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 712.5 GRAMS DATE AND TIME OF NECROPSY: 02/02/00 8:02 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SEMINAL VESICLES (SV) : -LARGE; TRIMMING COMMENT: LEFT: 40 X 15 X 5 MM (RIGHT IS 25 X 11 X 5 MM) LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT: 14 X 9 X 5 MM (LEFT IS 5 X 3 X 2 MM); RIGHT APPEARS CYSTIC; (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST BROWN MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; BILATERAL: MOIST CLEAR MATERIAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATELY SEVERE, MULTI-FOCAL 4445 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 280 ANIMAL NUMBER: C92302 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/02/00 STUDY DAY OF DEATH: 668 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 712.5 GRAMS DATE AND TIME OF NECROPSY: 02/02/00 8:02 PROSECTOR: JODI THIEREN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4446 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 281 ANIMAL NUMBER: C92303 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 685.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:01 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0356 .0274 2.3473 1.9623 1.8960 16.8091 2.1910 1.6829 1.5796 1.5913 .0104 .0155 .0052 % .0040 % .3426 % .2864 % .2767 % 2.4535 % .3198 % .2456 % .2306 % .2323 % .0015 % .0023 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0152 .0117 1.0000 .8360 .8077 7.1610 .9334 .7170 .6729 .6779 .0044 .0066 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED SPLEEN (SP) : -LIGHT FOCUS(I)/AREA(S); PARIETAL SURFACE: SINGLE GRAY AREA, 2 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL SPLEEN (SP) : ^UNREMARKABLE DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4447 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 282 ANIMAL NUMBER: C92303 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 685.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:01 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4448 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 283 ANIMAL NUMBER: C92304 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 871.9 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 13:00 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK 93; 35 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN -DARK FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK BROWN FOCI, PINPOINT AND UP TO 1 MM IN DIAMETER AUDITORY SEB GL (AS) : -MASS(ES); LEFT: SINGLE MASS, 45 X 35 X 27 MM, RED, DARK RED, TAN, GREEN, LOBULATED, VASCULAR, AND FIRM; ON SECTION EXUDES A LIGHT GREEN, CASEOUS SEMISOLID; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 20) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL OBESE HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, MULTI FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT DEATH COMMENT (DC) : -AUDITORY SEBACEOUS GLAND CARCINOMA,PRESENT AUDITORY SEB GL (AS) : -M-CARCINOMA,-NEOPLASM PRESENT 4449 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 284 ANIMAL NUMBER: C92304 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 871.9 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 13:00 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; LEFT: DARK RED, DRY, CRUSTED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE), LN, MANDIBULAR (MN) 4450 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 285 ANIMAL NUMBER: C92305 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSALCERVICAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; HEAD-ENTIRE; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MASS(ES); RIGHT MEDIAN LOBE: SINGLE MASS, 28 X 21 X 12 MM, TAN, RED, VASCULAR AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (HEPATIC) NOT IDENTIFIED -LARGE; ALL LOBES: MARGINS ROUNDED SKIN (SK) : -CRUSTED AREA(S); MID VENTRAL CERVICAL, MID DORSAL THORACIC AND HEAD: MULTIPLE TAN CRUSTED AREAS, UP TO 5 MM IN DIAMETER (CAP 18); POSSIBLY CONSISTENT WITH ALL SORES NOTED IN CLINICAL OBSERVATIONS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 7 X 5 MM, DARK RED, TAN AND FIRM HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT SKIN (SK) : -ATROPHY, ADNEXAL,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4451 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 286 ANIMAL NUMBER: C92305 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 5 X 3 MM LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 6 X 3 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -GI TRACT-DARK FLUID CONTENT; DUODENUM AND JEJUNUM: CONTAINS A BLACK SEMISOLID MATERIAL STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL -ULCERATION,-SEVERE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL 4452 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 287 ANIMAL NUMBER: C92305 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 14:31 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), TESTIS (TE) 4453 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 288 ANIMAL NUMBER: C92306 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/00 7:56 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PANCREAS (PA) : -MASS(ES); NEAR SPLEEN: SINGLE MASS, 5 X 5 X 4 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (PANCREATIC) NOT IDENTIFIED THYROID (TY) : -LARGE; BILATERAL: LEFT IS 6 X 5 X 4 MM, RIGHT IS 5 X 3 X 3 MM PARATHYROID (PT) : -LARGE; LEFT: 1 MM IN DIAMETER (RIGHT IS LESS THAN 1 MM IN DIAMETER) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 MM IN DIAMETER, RED, TAN AND SOFT RED, DARK HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >UNREMARKABLE TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL BRAIN (BR) : >UNREMARKABLE PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4454 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 289 ANIMAL NUMBER: C92306 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/00 7:56 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PROSTATE (PR) : -NODULAR; PROXIMAL TO URETHRA: TAN GREEN AND FIRM PROSTATE (PR) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) 4455 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 290 ANIMAL NUMBER: C92307 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 447.0 GRAMS DATE AND TIME OF NECROPSY: 03/12/00 9:33 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); DORSAL; WEEK 59; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); NOSE; WEEK 100; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); HEAD-MAXILLARY-LEFT; WEEK 100; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY PANCREAS (PA) : -MASS(ES); NEAR SPLEEN: SINGLE MASS, 3 MM IN DIAMETER, TAN AND SOFT (CAP 28); REGIONAL LN (MESENTERIC) NORMAL THYROID (TY) : -MASS(ES); INVOLVING ENTIRE LEFT THYROID: SINGLE MASS, 7 X 5 X 4 MM, TAN AND FIRM; COLLECTED INTACT; REGIONAL LN (MEDIASTINAL) NORMAL (CAP 26) SKIN (SK) : -CRUSTED AREA(S); DORSAL LUMBAR REGION: TWO DRY TAN CRUSTED AREAS, ONE IS 15 X 12 MM (CAP 22), THE OTHER IS 4 MM IN DIAMETER (CAP 23) HISTOPATHOLOGY LUNG (LU) : -INFLAMMATION, GRANULOMATOUS,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : -B-KERATOACANTHOMA,- 2 NEOPLASMS PRESENT 4456 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 291 ANIMAL NUMBER: C92307 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 447.0 GRAMS DATE AND TIME OF NECROPSY: 03/12/00 9:33 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 10 X 6 MM, DARK BROWN, DARK RED AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, PINPOINT AND UP TO 1 MM IN DIAMETER PROSTATE (PR) : -NODULAR; PROXIMAL, SURROUNDING URETHRA: GREENISH-TAN SEMINAL VESICLES (SV) : -GELATINOUS; BILATERAL: TAN CALVARIUM/SKULL (CA) : -MASS(ES); LEFT NASAL REGION: SINGLE MASS, 10 X 10 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (MANDIBULAR) NORMAL (CAP 24) BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE PROSTATE (PR) : -HEMORRHAGE,-MINIMAL, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL SEMINAL VESICLES (SV) : -EDEMA,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -OSTEOGENIC SARCOMA,-PRESENT CALVARIUM/SKULL (CA) : -M-SARCOMA, OSTEOGENIC,-NEOPLASM PRESENT ADIPOSE TISSUE (AT) : -FAT NECROSIS,-MODERATE, MULTI-FOCAL 4457 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 292 ANIMAL NUMBER: C92307 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 447.0 GRAMS DATE AND TIME OF NECROPSY: 03/12/00 9:33 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADIPOSE TISSUE (AT) : -MASS(ES); NEAR LEFT CAUDAL EPIDIDYMIS: SINGLE MASS, 5 X 5 X 3 MM, TAN AND SOFT; SAME ON CUT SURFACE (CAP 25); REGIONAL LN (ILIAC) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -GI TRACT-LARGE LUMEN; STOMACH: UP TO 50 X 30 X 30 MM, DISTENDED WITH GAS -DISCHARGE-OCULAR; LEFT: MOIST DARK BROWN MATERIAL -DISCHARGE-NASAL; MOIST BROWN MATERIAL -ANIMAL THIN -TEETH MISSING/BROKEN/WORN; LEFT UPPER INCISOR: BROKEN AT BASE 4458 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 293 ANIMAL NUMBER: C92307 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/12/00 STUDY DAY OF DEATH: 707 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 447.0 GRAMS DATE AND TIME OF NECROPSY: 03/12/00 9:33 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: TESTIS (TE), LN, MESENTERIC (MS), LN, MANDIBULAR (MN), LN, MEDIASTINAL (ML) 4459 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 294 ANIMAL NUMBER: C92308 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 535.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:41 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -MOTTLED; ALL LOBES: DARK RED AND TAN KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL: MULTIPLE TAN AND FIRM GRANULAR MATERIAL, UP TO 1 MM IN DIAMETER -DIFFUSELY LIGHT; BILATERAL: TAN LIVER (LI) : -MOTTLED; ALL LOBES: DARK RED AND TAN -LARGE; ALL LOBES: MARGINS ROUNDED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LUNG (LU) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATELY SEVERE, MULTI-FOCAL LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT 4460 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 295 ANIMAL NUMBER: C92308 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 535.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:41 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), TESTIS (TE), URINARY BLADDER (UB) 4461 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 296 ANIMAL NUMBER: C92309 SEX: MALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 558.5 GRAMS PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -DIFFUSELY LIGHT; BILATERAL: TAN LIVER (LI) : -MASS(ES); DISTAL END OF LEFT LATERAL LOBE: SINGLE MASS, 45 X 45 X 25 MM, TAN, RED, VASCULAR AND FIRM; SAME ON CUT SURFACE; ENTIRE LOBE COLLECTED; REGIONAL LN (HEPATIC) NORMAL (CAP 22) STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SEVERE, DIFFUSE LIVER (LI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT TESTIS (TE) : -MINERALIZATION,-MINIMAL, FOCAL STOMACH, GL (ST) : -EDEMA,-MODERATELY SEVERE, FOCAL -EROSION,-MINIMAL, FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT 4462 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 297 ANIMAL NUMBER: C92309 SEX: MALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 558.5 GRAMS PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, OTHER (LN) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>HEPATIC LYMPH NODE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA) 4463 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 298 ANIMAL NUMBER: C92310 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/25/99 STUDY DAY OF DEATH: 507 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/25/99 12:48 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL THYMUS (TH) : -LARGE; 15 X 9 X 5 MM PROSTATE (PR) : -MASS(ES); INVOLVING ENTIRE PROSTATE: SINGLE MASS, 32 X 23 X 16 MM, TAN, RED, DARK RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE LUMBAR) LN, LUMBAR/ILIAC (LM) : -MASS(ES); INVOLVING BILATERAL LUMBAR AND ILIAC LN: SINGLE MASS, 35 X 28 X 9 MM, TAN, RED AND GREEN; SAME ON CUT SURFACE; COLLECTED HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -DILATATION, PELVIC,-PRESENT LIVER (LI) : -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL THYMUS (TH) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PROSTATE (PR) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DEATH COMMENT (DC) : -HISTIOCYTIC SARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 4464 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 299 ANIMAL NUMBER: C92310 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/25/99 STUDY DAY OF DEATH: 507 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/25/99 12:48 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4465 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 300 ANIMAL NUMBER: C92311 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1008.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:20 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; SOME ARE SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTION TESTIS (TE) : -RED/YELLOW FOCUS(I)/AREA(S); LEFT, BENEATH TUNICA: MULTIPLE AREAS, UP TO 8 X 5 MM PITUITARY (PI) : -RED FOCUS(I)/AREA(S); MID ASPECT: SINGLE DARK RED AREA, 1 MM IN DIAMETER STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL TESTIS (TE) : -ATROPHY/DEGENERATION,-SLIGHT, MULTI FOCAL -CYST,-PRESENT -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE URINARY BLADDER (UB) : -CALCULUS,-PRESENT 4466 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 301 ANIMAL NUMBER: C92311 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 1008.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:20 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY) 4467 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 302 ANIMAL NUMBER: C92312 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 455.9 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 13:25 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); BILATERAL, HINDFEET: SINGLE RED, TAN AND DRY AREA ON EACH, BOTH ARE 4 MM IN DIAMETER; BOTH COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS HISTOPATHOLOGY DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT FOOT/FOOTPAD (FP) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL 4468 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 303 ANIMAL NUMBER: C92312 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/28/99 STUDY DAY OF DEATH: 449 STUDY WEEK OF DEATH: 65 TERMINAL BODY WEIGHT: 455.9 GRAMS DATE AND TIME OF NECROPSY: 06/28/99 13:25 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4469 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 304 ANIMAL NUMBER: C92313 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 674 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 7:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); DORSAL; WEEK 72; 35 X 40MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -MASS(ES); DORSAL LUMBAR REGION: SINGLE MASS, 50 X 38 X 15 MM, RED, DARK RED, TAN, FRIABLE AND FIRM; SAME ON CUT SURFACE (CAP 20); REGIONAL LN (INGUINAL) NOT IDENTIFIED MAMMARY, MALE (MM) : -CYST(S); VENTRAL CERVICAL REGION: MULTIPLE TAN, WHITE AND CREAMY SEMI FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER (CAP 19) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL SKIN (SK) : -B-KERATOACANTHOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT MAMMARY, MALE (MM) : -GALACTOCELE,-MODERATE, MULTI-FOCAL 4470 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 305 ANIMAL NUMBER: C92313 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 02/08/00 STUDY DAY OF DEATH: 674 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 02/08/00 7:36 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), TESTIS (TE) 4471 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 306 ANIMAL NUMBER: C92314 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 515.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 8:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY SKIN (SK) : -CRUSTED AREA(S); MID DORSAL THORACIC REGION: SINGLE DARK RED AND DRY AREA, 7 X 6 X 4 MM (CAP 19) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 9 X 9 MM, DARK RED, RED, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, DIFFUSE THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -HYPERPLASIA, FOLLICULAR CELL,MODERATE, FOCAL SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4472 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 307 ANIMAL NUMBER: C92314 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/14/00 STUDY DAY OF DEATH: 649 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: 515.5 GRAMS DATE AND TIME OF NECROPSY: 01/14/00 8:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, UPPER INCISORS -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), TESTIS (TE) 4473 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 308 ANIMAL NUMBER: C92315 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 731.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:24 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0306 .0311 2.3117 1.8457 1.9516 19.3215 2.2745 .9082 1.8018 1.7817 .0166 .0199 .0042 % .0043 % .3161 % .2524 % .2669 % 2.6421 % .3110 % .1242 % .2464 % .2436 % .0023 % .0027 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0132 .0135 1.0000 .7984 .8442 8.3581 .9839 .3929 .7794 .7707 .0072 .0086 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4474 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 309 ANIMAL NUMBER: C92315 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 731.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:24 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), TESTIS (TE) 4475 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 310 ANIMAL NUMBER: C92316 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 512.6 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 12:56 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 84; 30 X 40MM; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -DIFFUSELY LIGHT; LIVER (LI) : -DIFFUSELY LIGHT; BILATERAL: TAN ALL LOBES: TAN HEART (HT) : -LIGHT FOCUS(I)/AREA(S); PERICARDIUM, DIFFUSE: MULTIPLE GREY AREAS, UP TO 10 X 6 MM SPLEEN (SP) : -LARGE; 63 X 16 X 10 MM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN AREA, 1 MM IN DIAMETER LN, LUMBAR/ILIAC (LM) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 8 X 5 X 5 MM (CAP 23) HISTOPATHOLOGY KIDNEY (KD) : >UNREMARKABLE LIVER (LI) : >UNREMARKABLE SKIN (SK) : -EROSION,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, FOCAL -M-FIBROSARCOMA,-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, FOCAL -FIBROSIS,-SLIGHT, FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATELY SEVERE, DIFFUSE STOMACH, GL (ST) : -EROSION,-MINIMAL, FOCAL DEATH COMMENT (DC) : -FIBROSARCOMA,-PRESENT LN, LUMBAR/ILIAC (LM) : -DILATATION, SINUSOIDAL,-MODERATE, MULTI-FOCAL 4476 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 311 ANIMAL NUMBER: C92316 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 512.6 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 12:56 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, MALE (MM) : -MASS(ES); RIGHT VENTRAL ABDOMINAL REGION: SINGLE MASS, 40 X 40 X 20 MM, TAN, RED, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED INTACT; ALL OVERLYING SKIN IS RED, MOIST, AND ULCERATED; REGIONAL LN (INGUINAL) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN MAMMARY, MALE (MM) : >TISSUE MISSING >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS FIBROSARCOMA THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), CAVITY, ABDOM (PC), TAIL URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (TI), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), TESTIS (TE) 4477 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 312 ANIMAL NUMBER: C92317 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 834.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:24 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0336 .0277 2.5770 2.7254 2.7560 26.2758 2.2535 1.4602 1.9470 1.9777 .0163 .0323 .0040 % .0033 % .3088 % .3266 % .3302 % 3.1483 % .2700 % .1750 % .2333 % .2370 % .0020 % .0039 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0130 .0107 1.0000 1.0576 1.0695 10.1963 .8745 .5666 .7555 .7674 .0063 .0125 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, MULTI FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4478 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 313 ANIMAL NUMBER: C92317 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 834.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:24 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), TESTIS (TE) 4479 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 314 ANIMAL NUMBER: C92318 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 789.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:48 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NONFORMED FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS -DIFFUSELY LIGHT; ALL LOBES: TAN TESTIS (TE) : -SMALL; RIGHT: 9 X 10 X 18 MM (LEFT IS 13 X 14 X 25 MM) -RED/YELLOW FOCUS(I)/AREA(S); LEFT, BENEATH TUNICA: MULTIPLE AREAS, UP TO 5 X 7 MM PITUITARY (PI) : -CYST(S); LEFT ASPECT: SINGLE CYST, 2 MM IN DIAMETER, FILLED WITH A DARK RED FLUID HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT TESTIS (TE) : -ATROPHY/DEGENERATION,-SEVERE, MULTI FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT URINARY BLADDER (UB) : -CALCULUS,-PRESENT 4480 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 315 ANIMAL NUMBER: C92318 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 789.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:48 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) 4481 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 316 ANIMAL NUMBER: C92319 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 649.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:09 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC-RIGHT; WEEK 82; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -MASS(ES); RIGHT THORACIC REGION: SINGLE MASS, 15 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23) PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT TESTIS (TE) : -B-INTERSTITIAL CELL TUMOR,-NEOPLASM PRESENT SKIN (SK) : -ATROPHY, ADNEXAL,-MODERATE, FOCAL -B-FIBROMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4482 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 317 ANIMAL NUMBER: C92319 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 649.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:09 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), URINARY BLADDER (UB), LN, AXILLARY (AX) 4483 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 318 ANIMAL NUMBER: C92320 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 717.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:15 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0399 .0353 2.3881 1.9368 2.1021 18.0864 1.8416 1.0452 1.6298 1.5695 .0240 .0300 .0056 % .0049 % .3331 % .2701 % .2932 % 2.5225 % .2568 % .1458 % .2273 % .2189 % .0033 % .0042 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0167 .0148 1.0000 .8110 .8802 7.5736 .7712 .4377 .6825 .6572 .0100 .0126 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, FOLLICULAR,-SLIGHT, FOCAL 4484 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 319 ANIMAL NUMBER: C92320 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 717.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:15 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE) 4485 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 320 ANIMAL NUMBER: C92321 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:00 PROSECTOR: JAMES T. CALLEN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 X 3 MM; COLLECTED ON ROUTINE SECTIONS SKIN (SK) : -ABRASION(S)/ULCERATION(S); MID PERIANAL REGION: MULTIPLE TAN, MOIST ABRADED AREAS, UP TO 4 MM IN DIAMETER (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT SKIN (SK) : -EROSION,-MODERATE, FOCAL -FIBROSIS,-MODERATE, FOCAL -INFLAMMATION, CHRONIC-ACTIVE,MODERATELY SEVERE, MULTI-FOCAL URINARY BLADDER (UB) : -CALCULUS,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4486 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 321 ANIMAL NUMBER: C92321 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 570.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:00 PROSECTOR: JAMES T. CALLEN RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE) 4487 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 322 ANIMAL NUMBER: C92322 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 764.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -HYPERPLASIA, FOLLICULAR CELL,MODERATE, FOCAL TESTIS (TE) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4488 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 323 ANIMAL NUMBER: C92322 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 764.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:17 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) 4489 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 324 ANIMAL NUMBER: C92323 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:04 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; LIMITED USE; LIMB-HINDLEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); LEFT -ROUGH SURFACE, DIFFUSE; BILATERAL -LARGE; BILATERAL: EACH ARE 30 X 20 X 14 MM LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); LEFT LATERAL LOBE: SINGLE TAN LINEAR AREA, 8 X 2 MM; COLLECTED ON ROUTINE SECTION -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS PANCREAS (PA) : -MASS(ES); DIFFUSE: MULTIPLE MASSES, UP TO 10 MM IN DIAMETER, TAN AND FIRM; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (SEE PANCREATIC) THYROID (TY) : -LARGE; BILATERAL: EACH IS 9 X 5 X 3 MM HISTOPATHOLOGY KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATELY SEVERE, DIFFUSE LIVER (LI) : -FIBROSIS, CAPSULAR,-SLIGHT, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA, ACINAR CELL,-NEOPLASM PRESENT -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : >UNREMARKABLE 4490 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 325 ANIMAL NUMBER: C92323 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:04 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SPLEEN (SP) : -LARGE; 51 X 14 X 8 MM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE DARK BROWN LINEAR AREA, 3 X 1 MM -THICKENED WALL; MUCOSA, DIFFUSE AORTA (AO) : -LARGE; DIFFUSE: 3 MM IN DIAMETER; COLLECTED LN, LUMBAR/ILIAC (LM) : -LARGE; LEFT, LUMBAR: 15 X 9 X 4 MM (RIGHT NOT IDENTIFIED) (CAP 26) BILE DUCT (BD) : -LARGE; DIFFUSE: 2 MM IN DIAMETER 23) LN, ANT MES/PANC (AP) : -DIFFUSELY RED; DARK RED (CAP 25) (CAP TESTIS (TE) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL SPLEEN (SP) : >UNREMARKABLE STOMACH, GL (ST) : -EROSION,-MINIMAL, FOCAL MULTI DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT AORTA (AO) : >UNREMARKABLE LN, LUMBAR/ILIAC (LM) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT BILE DUCT (BD) : >UNREMARKABLE LN, ANT MES/PANC (AP) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 4491 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 326 ANIMAL NUMBER: C92323 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 819.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:04 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), LN, RENAL (RL), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), AUDITORY SEB GL (AS), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) 4492 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 327 ANIMAL NUMBER: C92324 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 671.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) .0297 .0263 2.3771 2.4922 2.7645 20.7937 2.1598 1.2150 1.8989 1.9085 .0190 .0172 .0044 % .0039 % .3543 % .3714 % .4120 % 3.0989 % .3219 % .1811 % .2830 % .2844 % .0028 % .0026 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0125 .0111 1.0000 1.0484 1.1630 8.7475 .9086 .5111 .7988 .8029 .0080 .0072 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL PANCREAS (PA) : -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4493 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 328 ANIMAL NUMBER: C92324 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 671.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), TESTIS (TE) 4494 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 329 ANIMAL NUMBER: C92325 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 591.5 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:48 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 20.3850 ------------------------------------------- ------------------------------------ 3.4463 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4495 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 330 ANIMAL NUMBER: C92325 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 591.5 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:48 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4496 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 331 ANIMAL NUMBER: C92326 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 491.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:37 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4497 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 332 ANIMAL NUMBER: C92327 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 489.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:13 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 14.0186 ------------------------------------------- ------------------------------------ 2.8633 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4498 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 333 ANIMAL NUMBER: C92327 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 489.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:13 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4499 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 334 ANIMAL NUMBER: C92328 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 492.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:54 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 15.1771 ------------------------------------------- ------------------------------------ 3.0810 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4500 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 335 ANIMAL NUMBER: C92328 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 492.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:54 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4501 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 336 ANIMAL NUMBER: C92329 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:16 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 10.4029 ------------------------------------------- ------------------------------------ 3.6450 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4502 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 337 ANIMAL NUMBER: C92329 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 285.4 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:16 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4503 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 338 ANIMAL NUMBER: C92330 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:54 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) SPLEEN (SP) LEFT TESTIS (TE0) RIGHT TESTIS (TE1) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) ------------------------------------ 17.2000 ------------------------------------------- ------------------------------------ 3.0676 % ------------------------------------------- ------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4504 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 339 ANIMAL NUMBER: C92330 SEX: MALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 560.7 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:54 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), TESTIS (TE), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), PROSTATE (PR), SEMINAL VESICLES (SV), EPIDIDYMIDES (EP), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), MAMMARY, MALE (MM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4505 COV/HH> THE DEVELOPMENT SERVICES COMPANY Sponsor: 3M S t. P a u l, M in n e s o ta Covance 6329-212 FINAL REPORT Study Title: 1 0 4 -W ee k D ie ta ry C a rc in o g e n ic ity S tu d y w ith N a rro w R a n g e (9 8 .1 % ) N -E th y l P e rflu o ro o c ta n e su lfo n a m id o E th a n o l in R a ts Author: P e te r J. T h o m fo rd , P h D Study Completion Date: D ecem b er 13, 2001 Testing Facility: C o v a n c e L a b o ra to rie s Inc. 3301 K in sm an B o u lev ard M ad iso n , W isc o n sin 5 3 7 0 4 -2 5 9 5 Laboratory Study Identification: C ovance 6329-228 Sponsor Study Identification: 3 M T -6 3 1 6 .1 V o lu m e X II o f X II Page 4506 of4873 Covance 6329-212 Covance 6329-228 3M T-6316.1 VOLUME XII OF XII CONTENTS Page A P P E N D I X 6 ( C o n t i n u e d ) .................................................................................................................................................... 4 5 0 8 I n d i v i d u a l A n a t o m i c P a t h o l o g y D a t a .................................................................................................................... 4 5 0 9 A P P E N D I X 7 ......................................................................................................................................................................................4 8 5 7 C e l l P r o l i f e r a t i o n R e p o r t .................................................................................................................................................. 4 8 5 8 4507 APPENDIX 6 (Continued) In d iv id u a l A n a to m ic P a th o lo g y D a ta Covance 6329-212 Covance 6329-228 3M T-6316.1 4508 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 340 ANIMAL NUMBER: C92331 SEX: FEMALE DATE OF DEATH: 01/06/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/06/00 15:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 273.0 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 42; 60 X 40MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >RESPIRATION; IRREGULAR; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LLOBES: TAN AND FIRM MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 75 X 40 X 15 MM, RED, DARK RED, GREEN, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 35 X 25 X 25 MM, RED, DARK RED, TAN AND BROWN; ON SECTION, EXUDES A BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,- 2 NEOPLASMS PRESENT EYE (EY) : -INFLAMMATION, ACUTE, CILIARY,MODERATELY SEVERE, FOCAL 4509 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 341 ANIMAL NUMBER: C92331 SEX: FEMALE DATE OF DEATH: 01/06/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/06/00 15:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 273.0 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) LN, MESENTERIC (MS) : -DIFFUSELY RED; DARK RED PITUITARY (PI) : -MASS(ES); 11 X 8 X 6 MM, RED, DARK RED, GREY, TAN AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: GREEN MATERIAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT MUSCLE, SKELETAL (SM) : -MINERALIZATION,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -HYPERPLASIA, MEDULLARY,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE 4510 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 342 ANIMAL NUMBER: C92331 SEX: FEMALE DATE OF DEATH: 01/06/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/06/00 15:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 273.0 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DARK YELLOW AND BROWN -DISCHARGE-OCULAR; BILATERAL: RED -ANIMAL THIN DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4511 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 343 ANIMAL NUMBER: C92331 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 273.0 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 15:06 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), NERVE, SCIATIC (SN), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), HEMATO NEOPLASIA (HN) 4512 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 344 ANIMAL NUMBER: C92332 SEX: FEMALE DATE OF DEATH: 04/28/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/28/98 8:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 185.7 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.0862 -------------------------------------------------- ------------------------------------ 3.8159 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4513 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 345 ANIMAL NUMBER: C92332 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 185.7 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:56 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4514 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 346 ANIMAL NUMBER: C92333 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 580.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:20 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 9 X 6 X 6 MM (LEFT IS 4 X 4 X 2 MM) OVARY (OV) : -CYST(S); LEFT: SINGLE DARK RED FLUID FILLED CYST, 4 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL RECTUM (RE) : -PARASITISM,-PRESENT UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4515 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 347 ANIMAL NUMBER: C92333 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 580.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:20 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4516 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 348 ANIMAL NUMBER: C92334 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 11:21 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 656.7 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE DARK RED AREA, 4 X 3 MM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -LARGE; 7 X 6 X 3 MM -DIFFUSELY RED; DARK RED STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BLACK AREAS, UP TO 3 X 2 MM HISTOPATHOLOGY LUNG (LU) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : >UNREMARKABLE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE 4517 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 349 ANIMAL NUMBER: C92334 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 656.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:21 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (OV), 4518 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 350 ANIMAL NUMBER: C92335 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/29/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/29/98 13:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; MOUTH; VERIFIED AT NECROPSY: OBSERVED >SACRIFICE; SACRIFICE APPROVED BY; VETERINARIAN; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY CAVITY, ORAL (OC) : -ABRASION(S)/ULCERATION(S); TISSUE SURROUNDING BILATERAL, UPPER INCISORS: SINGLE DARK RED ABRADED AREA, 5 X 4 MM; TISSUE APPEARS SWOLLEN; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: UPPER INCISORS HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL DEATH COMMENT (DC) : -ORAL EROSION,-PRESENT CAVITY, ORAL (OC) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL 4519 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 351 ANIMAL NUMBER: C92335 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/29/98 STUDY DAY OF DEATH: 54 STUDY WEEK OF DEATH: 8 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/29/98 13:05 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4520 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 352 ANIMAL NUMBER: C92336 SEX: FEMALE DATE OF DEATH: 10/27/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/27/99 14:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 358.9 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 59; 60 X 70MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 75; 30 X 35MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT MEDIAN LOBE, ALONG MARGIN: SINGLE TAN AREA, 6 X 4 MM (CAP 20) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 46 X 35 X 21 MM, RED, DARK RED, GRAY, TAN, VASCULAR, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 19); OVERLYING SKIN HAS A SINGLE, DRY, TAN, AND BROWN ULCERATED AREA, 32 X 32 MM; COLLECTED INTACT WITH MASS HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 4521 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 353 ANIMAL NUMBER: C92336 SEX: FEMALE DATE OF DEATH: 10/27/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/27/99 14:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 358.9 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 18) -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 21 X 18 MM, GRAY, TAN, LOBULATED, AND SOFT; ON SECTION, EXUDES A GREEN CASEOUS MATERIAL; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 19); OVERLYING SKIN HAS A SINGLE DRY, BROWN CRUSTED AREA, 20 X 18 MM; COLLECTED INTACT WITH MASS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE ; LEFT:: 7 MM IN DIAMETER (RIGHT IS 4 X 4 X 3 MM) BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MINIMAL, MULTI-FOCAL 4522 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 354 ANIMAL NUMBER: C92336 SEX: FEMALE DATE OF DEATH: 10/27/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/27/99 14:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 358.9 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 5 MM, RED, DARK RED, GRAY, LOBULATED, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL 4523 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 355 ANIMAL NUMBER: C92336 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/27/99 STUDY DAY OF DEATH: 570 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 358.9 GRAMS DATE AND TIME OF NECROPSY: 10/27/99 14:40 PROSECTOR: JOHN S. HALFORD RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SE), (CE), 4524 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 356 ANIMAL NUMBER: C92337 SEX: FEMALE DATE OF DEATH: 10/26/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/26/99 14:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 410.3 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 50; 35 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 76; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 4 X 3 MM; COLLECTED ON ROUTINE SECTIONS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 45 X 30 X 21 MM, RED, DARK RED, GRAY, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE INGUINAL) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 18); POSSIBLY CONSISTENT WITH MASS (RIGHT INGUINAL REGION) AS NOTED IN CLINICAL OBSERVATIONS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL LIVER (LI) : ^UNREMARKABLE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -NECROSIS,-MODERATELY SEVERE, FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT 4525 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 357 ANIMAL NUMBER: C92337 SEX: FEMALE DATE OF DEATH: 10/26/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/26/99 14:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 410.3 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -SMALL; BILATERAL: EACH IS 4 X 3 X 3 MM; RIGHT IS GELATINOUS PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, GRAY, TAN AND SOFT OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER LN, INGUINAL (IN) : -LARGE; LEFT: UP TO 6 X 5 X 4 MM (RIGHT NOT IDENTIFIED); SOME APPEAR FIRM (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL BRAIN (BR) : -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, MINIMAL, FOCAL OVARY (OV) : >UNREMARKABLE ENDOMETRIAL,- DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, INGUINAL (IN) : -N-MAMMARY CARCINOMA,-NEOPLASM PRESENT 4526 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 358 ANIMAL NUMBER: C92337 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/26/99 STUDY DAY OF DEATH: 569 STUDY WEEK OF DEATH: 82 TERMINAL BODY WEIGHT: 410.3 GRAMS DATE AND TIME OF NECROPSY: 10/26/99 14:35 PROSECTOR: JOHN S. HALFORD RECORDER: LYNN HORNER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SE), (CE), 4527 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 359 ANIMAL NUMBER: C92338 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 302.4 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRALCERVICAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 12 X 14 X 25 MM, FIRM, LOBULATED, AND TAN; SAME ON CUT SURFACE (CAP 18); REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 13 MM, SOFT, RED, AND DARK RED HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4528 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 360 ANIMAL NUMBER: C92338 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 302.4 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE VENTRAL SURFACE: DRY RED MATERIAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4529 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 361 ANIMAL NUMBER: C92338 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 302.4 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 14:20 PROSECTOR: DENNIS HOFFMAN RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4530 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 362 ANIMAL NUMBER: C92339 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 10:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 66; 90 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 111 X 80 X 35 MM (153.0 GRAMS), RED, TAN, LOBULATED, VASCULAR, AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL RIGHT) NOTED IN CLINICAL OBSERVATIONS HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MODERATE, FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT 4531 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 363 ANIMAL NUMBER: C92339 SEX: FEMALE DATE OF DEATH: 01/17/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/17/00 10:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE BROWN AREAS, UP TO 2 X 1 MM -LARGE; 7 X 7 X 3 MM; APPEARS SWOLLEN PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL DUODENUM (DU) : >AUTOLYTIC JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT 4532 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 364 ANIMAL NUMBER: C92339 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/17/00 STUDY DAY OF DEATH: 652 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/17/00 10:40 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4533 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 365 ANIMAL NUMBER: C92340 SEX: FEMALE DATE OF DEATH: 10/05/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/05/99 15:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 488.1 GRAMS PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 44; 100 X 85MM; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 74; 35 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 74; 35 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; BLACK SKIN; VENTRALTHORACIC-LEFT; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 79; 30 X 35MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -DIFFUSELY DARK; BILATERAL: BROWN MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 40 X 30 X 20 MM, RED, DARK RED, TAN, AND SOFT; ON SECTION EXUDES A BROWN SEMIFLUID (CAP 20); REGIONAL LN (SEE AXILLARY) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 90 X 75 X 45 MM (144.2 GRAMS), RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE (CAP 18,21); REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -PYELONEPHRITIS,-SLIGHT, FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL THYROID (TY) : -MINERALIZATION,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 4534 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 366 ANIMAL NUMBER: C92340 SEX: FEMALE DATE OF DEATH: 10/05/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/05/99 15:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 488.1 GRAMS PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; VENTRAL ABDOMINAL REGION: SINGLE MASS, 60 X 40 X 20 MM, RED, DARK RED, TAN, AND SOFT; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A BROWN SEMIFLUID (CAP 22); REGIONAL LN (SEE AXILLARY) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LIGHT FOCUS (I)/AREA(S); BILATERAL: MULTIPLE TAN PINPOINT FOCI PITUITARY (PI) : -MASS(ES); 11 X 7 X 7 MM, RED, DARK RED, TAN, AND SOFT LN, AXILLARY (AX) : -LARGE; LEFT: 13 X 7 X 4 MM (RIGHT IS 4 X 3 X 2 MM) (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED AND DRY -ANIMAL THIN BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : >UNREMARKABLE 4535 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 367 ANIMAL NUMBER: C92340 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/05/99 STUDY DAY OF DEATH: 548 STUDY WEEK OF DEATH: 79 TERMINAL BODY WEIGHT: 488.1 GRAMS DATE AND TIME OF NECROPSY: 10/05/99 15:40 PROSECTOR: JILL PAUS RECORDER: LAURIE J. SCHULLER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4536 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 368 ANIMAL NUMBER: C92341 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 505.0 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0350 .0283 2.1849 1.7424 1.8779 13.3619 1.5514 .0415 .0362 .5689 .0139 .0147 .9924 .0069 % .0056 % .4327 % .3450 % .3719 % 2.6459 % .3072 % .0082 % .0072 % .1127 % .0028 % .0029 % .1965 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0160 .0130 1.0000 .7975 .8595 6.1156 .7101 .0190 .0166 .2604 .0064 .0067 .4542 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 53; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED MAMMARY, FEMALE (MF) : -CYST(S); VENTRAL ABDOMINAL REGION: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 12 MM IN DIAMETER (CAP 19) KIDNEY (KD) : -CYST,-SLIGHT, MULTI-FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL 4537 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 369 ANIMAL NUMBER: C92341 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 505.0 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: COLLECTED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN DARK RED; ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, FOCAL -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : -EDEMA,-SLIGHT, MULTI-FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MINIMAL, MULTI-FOCAL -INFLAMMATION, ACUTE,-MINIMAL, MULTI FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4538 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 370 ANIMAL NUMBER: C92341 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 505.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:53 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (ST), 4539 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 371 ANIMAL NUMBER: C92342 SEX: FEMALE DATE OF DEATH: 03/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/15/99 11:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 344 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 268.1 GRAMS PROSECTOR: JILL PAUS RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT POSTERIOR ASPECT: SINGLE DARK BROWN AREA, 3 MM IN DIAMETER CALVARIUM/SKULL (CA) : -FRACTURE; AT FIRST RUGAE: SURROUNDING SOFT TISSUE IS DARK RED AND GELATINOUS; COLLECTED HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL THYROID (TY) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -FRACTURED CALVARIUM,-PRESENT CALVARIUM/SKULL (CA) : -CYST, KERATIN,-PRESENT -FRACTURE,-PRESENT -HEMORRHAGE,-MODERATELY SEVERE, FOCAL MULTI 4540 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 372 ANIMAL NUMBER: C92342 SEX: FEMALE DATE OF DEATH: 03/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/15/99 11:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 344 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 268.1 GRAMS PROSECTOR: JILL PAUS RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -STAINS-PERINEUM/PERIANAL; YELLOW, MOIST MATERIAL -GI TRACT-LARGE LUMEN; ENTIRE GI TRACT: DISTENDED WITH GAS -DISCHARGE-OCULAR; BILATERAL: RED, DRY MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL CALVARIUM/SKULL (CA) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 4541 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 373 ANIMAL NUMBER: C92342 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/99 STUDY DAY OF DEATH: 344 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 268.1 GRAMS DATE AND TIME OF NECROPSY: 03/15/99 11:31 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MEs /pANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4542 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 374 ANIMAL NUMBER: C92343 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 398.0 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 14:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); SHOULDER-RIGHT; WEEK 19; 65 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; SHOULDERRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 80 X 50 X 25 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A MOIST, RED, ULCERATED AREA, 25 MM IN DIAMETER; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NORMAL (CAP 18) HISTOPATHOLOGY KIDNEY (KD) : -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, MULTI-FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL SKIN (SK) : -EROSION,-MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -NECROSIS,-MODERATE, FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL 4543 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 375 ANIMAL NUMBER: C92343 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 398.0 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 14:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE DARK RED FOCUS, 1 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -GI TRACT-DARK FLUID CONTENT; ENTIRE GI TRACT: DARK BROWN, TENACIOUS SEMIFLUID ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT 4544 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 376 ANIMAL NUMBER: C92343 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/10/99 STUDY DAY OF DEATH: 400 STUDY WEEK OF DEATH: 58 TERMINAL BODY WEIGHT: 398.0 GRAMS DATE AND TIME OF NECROPSY: 05/10/99 14:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) 4545 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 377 ANIMAL NUMBER: C92344 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 375.3 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); TRIMMING COMMENT: ALONG CAUDAL MARGIN OF LEFT LOBE: SINGLE TAN AREA, 3 MM IN DIAMETER; SUBMITTED IN BLOCK 22 MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 8 X 7 MM, DARK RED AND SOFT HISTOPATHOLOGY LUNG (LU) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL 4546 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 378 ANIMAL NUMBER: C92344 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 375.3 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ILEUM (IL) : -LARGE LYMPHOID AREA(S); DIFFUSE: UP TO 5 X 2 MM; COLLECTED ON ROUTINE SECTION VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CONTAINS A LIGHT GREEN SEMIFLUID MATERIAL GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL ILEUM (IL) : >UNREMARKABLE URINARY BLADDER (UB) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATELY SEVERE, FOCAL VAGINA (VA) : -INFLAMMATION, ACUTE,-MODERATELY SEVERE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4547 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 379 ANIMAL NUMBER: C92344 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 375.3 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 8:40 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), OVARY (OV), CERVIX (CV), HEMATO NEOPLASIA (HN) 4548 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 380 ANIMAL NUMBER: C92345 SEX: FEMALE DATE OF DEATH: 10/28/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/28/98 14:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 206 STUDY WEEK OF DEATH: 30 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); BILATERAL -LIGHT FOCUS(I)/AREA(S); LEFT: MULTIPLE TAN AREAS, UP TO 10 X 7 MM CERVIX (CV) : -LARGE; 10 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -MINERALIZATION, TUBULAR,-MODERATE, MULTI-FOCAL -PYELONEPHRITIS,-SEVERE, MULTI-FOCAL LN, MESENTERIC (MS) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL CERVIX (CV) : >UNREMARKABLE DEATH COMMENT (DC) : -PYELONEPHRITIS,-PRESENT 4549 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 381 ANIMAL NUMBER: C92345 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/28/98 STUDY DAY OF DEATH: 206 STUDY WEEK OF DEATH: 30 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/28/98 14:06 PROSECTOR: DONIA MEYER RECORDER: DONIA MEYER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4550 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 382 ANIMAL NUMBER: C92346 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 247.5 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:35 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >WART LIKE LESION(S); NOSE; WEEK 46; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -CRUSTED AREA(S); TIP OF NOSE: SINGLE BROWN, DRY AND RAISED AREA, 5 X 3 X 3 MM (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (FOR COMPARISON, LEFT IS 4 MM IN DIAMETER) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 9 X 6 MM, DARK RED, BROWN AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL PARATHYROID (PT) : -FIBROSIS,-SLIGHT, MULTI-FOCAL SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4551 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 383 ANIMAL NUMBER: C92346 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 247.5 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:35 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; AND DRY -ANIMAL THIN : BILATERAL: DARK RED THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4552 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 384 ANIMAL NUMBER: C92347 SEX: FEMALE DATE OF DEATH: 03/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/01/99 14:26 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 330 STUDY WEEK OF DEATH: 48 TERMINAL BODY WEIGHT: 209.8 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BONE, STERNUM (SB) : -MISALIGNED; MANUBRIUM: EXTENDS INTO THORACIC CAVITY; COLLECTED INTACT WITH RIBCAGE BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 11 X 5 MM; DARK RED, GRAY TAN AND SOFT; EXTENDS UNDER LEFT TRIGEMINAL NERVE OVARY (OV) : -DIFFUSELY RED; BILATERAL: DARK RED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW MATERIAL MOIST HISTOPATHOLOGY KIDNEY (KD) : -CYST,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BONE, STERNUM (SB) : >UNREMARKABLE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : >TISSUE MISSING OVARY (OV) : >UNREMARKABLE DEATH COMMENT (DC) : -OSTEOMA,-PRESENT NASAL TURBINATE (NT) : -B-OSTEOMA,-NEOPLASM PRESENT 4553 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 385 ANIMAL NUMBER: C92347 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/01/99 STUDY DAY OF DEATH: 330 STUDY WEEK OF DEATH: 48 TERMINAL BODY WEIGHT: 209.8 GRAMS DATE AND TIME OF NECROPSY: 03/01/99 14:26 PROSECTOR: TERRI L. WILLIAMS RECORDER: KEVIN BILLINGS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -GI TRACT-LIGHT FLUID CONTENT; STOMACH: YELLOW SEMIFLUID THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4554 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 386 ANIMAL NUMBER: C92348 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 422.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:49 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 101; 50 X 45MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 100; 60 X 40MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY THYROID (TY) : >NOTE:>THYROID/ PARATHYROID TAKEN OFF TRACHEA/ ESOPHAGUS AT NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 60 X 48 X 33 MM, TAN, RED, AND FIRM; ON SECTION, EXUDES A WHITE CREAMY SEMIFLUID (CAP 24); REGIONAL LN (SEE INGUINAL) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTIONS HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT PARATHYROID (PT) : -HYPERPLASIA,-SLIGHT, DIFFUSE MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 4555 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 387 ANIMAL NUMBER: C92348 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 9:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 422.3 GRAMS PROSECTOR: JODI THIEREN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 54 X 40 X 25 MM, TAN, RED, FIRM AND LOBULATED; ON CUT SURFACE, EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (SEE INGUINAL) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYMUS (TH) : -MASS(ES); LEFT LOBE: SINGLE MASS, 7 X 6 X 5 MM, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE MEDIASTINAL) EYE (EY) : -FIBROSIS, CORNEAL,-SLIGHT, FOCAL -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, FOCAL -ULCERATION, CORNEAL,-MINIMAL, FOCAL BRAIN (BR) : >UNREMARKABLE LN, MESENTERIC (MS) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL THYMUS (TH) : -M-THYMIC CARCINOMA,-NEOPLASM PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL 4556 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 388 ANIMAL NUMBER: C92348 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 9:49 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 422.3 GRAMS PROSECTOR: JODI THIEREN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, RED, DARK RED, GRAY AND FIRM LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 22) -LARGE; BILATERAL: EACH IS UP TO 10 X 6 X 2 MM FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: SINGLE RED, TAN, MOIST CRUSTED AREA, 12 X 9 MM; COLLECTED LN, INGUINAL (IN) : -LARGE; LEFT: 10 X 6 X 3 MM (CAP 25) (RIGHT NOT IDENTIFIED) LN, MEDIASTINAL (ML) : -MOTTLED; RED AND TAN (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL RECTUM (RE) : -PARASITISM,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATELY SEVERE, DIFFUSE FOOT/FOOTPAD (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -FIBROSIS,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL LN, INGUINAL (IN) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATELY SEVERE, DIFFUSE LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4557 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 389 ANIMAL NUMBER: C92348 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 422.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:49 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL, LOWER INCISORS -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4558 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 390 ANIMAL NUMBER: C92349 SEX: FEMALE DATE OF DEATH: 09/28/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 09/28/99 14:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 9 X 8 MM, RED, DARK RED, TAN, GRAY AND SOFT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL: UPPER INCISORS HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4559 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 391 ANIMAL NUMBER: C92349 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 09/28/99 STUDY DAY OF DEATH: 541 STUDY WEEK OF DEATH: 78 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 09/28/99 14:00 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4560 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 392 ANIMAL NUMBER: C92350 SEX: FEMALE DATE OF DEATH: 01/21/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/21/00 14:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 325.5 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; AUDIBLE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; OTHER; RED ORAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 7 X 4 MM, DARK RED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL -DISCHARGE-ORAL; MOIST RED MATERIAL HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : >UNREMARKABLE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4561 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 393 ANIMAL NUMBER: C92350 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/21/00 STUDY DAY OF DEATH: 656 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 325.5 GRAMS DATE AND TIME OF NECROPSY: 01/21/00 14:09 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4562 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 394 ANIMAL NUMBER: C92351 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 13:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 417.8 GRAMS PROSECTOR: JODI THIEREN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY ADRENAL, CORTEX (AC) : -DARK FOCUS(I)/AREA(S); LEFT: SINGLE DARK BROWN FOCUS, 2 MM IN DIAMETER PITUITARY (PI) : -RAISED AREA(S); RIGHT ANTERIOR ASPECT: SINGLE TAN RAISED AREA, UP TO 1 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT MULTI 4563 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 395 ANIMAL NUMBER: C92351 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 417.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:40 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4564 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 396 ANIMAL NUMBER: C92352 SEX: FEMALE DATE OF DEATH: 04/28/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/28/98 8:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 215.4 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.8244 -------------------------------------------------- ------------------------------------ 3.6325 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4565 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 397 ANIMAL NUMBER: C92352 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 215.4 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:59 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4566 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 398 ANIMAL NUMBER: C92353 SEX: FEMALE DATE OF DEATH: 02/01/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/01/00 13:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 323.0 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; ATAXIC; MODERATE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; WARM TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY FLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER (CAP 18) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4567 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 399 ANIMAL NUMBER: C92353 SEX: FEMALE DATE OF DEATH: 02/01/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/01/00 13:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 323.0 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, RED, DARK RED, GREY AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW -DISCHARGE-OCULAR; LEFT: RED -DISCHARGE-NASAL; BILATERAL: RED ADRENAL, CORTEX (AC) : -HYPERTROPHY,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4568 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 400 ANIMAL NUMBER: C92353 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/01/00 STUDY DAY OF DEATH: 667 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 323.0 GRAMS DATE AND TIME OF NECROPSY: 02/01/00 13:44 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4569 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 401 ANIMAL NUMBER: C92354 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 385.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:11 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AND WHITE AREAS, UP TO 6 MM IN DIAMETER; SOME ARE SLIGHTLY RAISED; EXTRA SECTION (CAP 22) PANCREAS (PA) : -MASS(ES); ALONG DUODENUM: SINGLE MASS, 7 X 9 X 13 MM, FIRM, LOBULATED, RED, DARK RED AND TAN; SAME ON CUT SURFACE (CAP 24); REGIONAL LN (PANCREATIC) (CAP 25) SKIN (SK) : -ABRASION(S)/ULCERATION(S); RIGHT AXILLARY REGION: SINGLE DARK RED AND BROWN ABRADED AREA, 8 MM IN DIAMETER (CAP 23) HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,- 3 NEOPLASMS PRESENT PANCREAS (PA) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, ISLET CELL,- 3 NEOPLASMS PRESENT SKIN (SK) : >UNREMARKABLE 4570 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 402 ANIMAL NUMBER: C92354 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 11:11 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 385.5 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 8 X 11 MM, FIRM, RED, DARK RED AND TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS - SKIN/HAIRCOAT; HEAD AND ENTIRE VENTRAL SURFACE: DRY, RED MATERIAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -METAPLASIA, CARTILAGE,-MODERATE, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -HYPERPLASIA, GLANDULAR,-MODERATE, FOCAL UTERUS (UT) : -DILATATION,-MODERATE, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4571 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 403 ANIMAL NUMBER: C92354 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 385.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:11 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN), LN, ANT MES/PANC (AP) 4572 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 404 ANIMAL NUMBER: C92355 SEX: FEMALE DATE OF DEATH: 04/28/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/28/98 9:00 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 192.3 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.0644 -------------------------------------------------- ------------------------------------ 3.6736 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4573 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 405 ANIMAL NUMBER: C92355 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 192.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 9:00 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4574 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 406 ANIMAL NUMBER: C92356 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 368.0 GRAMS PROSECTOR: TODD ACKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0453 .0347 2.2412 1.3414 1.3549 10.1005 1.4449 .0361 .0422 .5418 .0110 .0140 .8728 .0123 % .0094 % .6090 % .3645 % .3682 % 2.7447 % .3926 % .0098 % .0115 % .1472 % .0030 % .0038 % .2372 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0202 .0155 1.0000 .5985 .6045 4.5067 .6447 .0161 .0188 .2417 .0049 .0062 .3894 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : -CYST(S); BILATERAL INGUINAL REGIONS: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 6 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL 4575 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 407 ANIMAL NUMBER: C92356 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 368.0 GRAMS PROSECTOR: TODD ACKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -DARK FOCUS(I)/AREA(S); LEFT ASPECT: SINGLE BLACK AREA, 3 X 1 MM -LARGE; 7 X 6 X 4 MM; APPEARS SWOLLEN GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL UPPER INCISORS: BROKEN 3 MM FORM BASE ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4576 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 408 ANIMAL NUMBER: C92356 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 368.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:31 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4577 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 409 ANIMAL NUMBER: C92357 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 8:47 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 605.8 GRAMS PROSECTOR: TODD ACKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0527 .0429 2.1032 1.7928 1.9039 17.4056 1.4364 .0462 .0343 .8142 .0212 .0180 1.3092 .0087 % .0071 % .3472 % .2959 % .3143 % 2.8732 % .2371 % .0076 % .0057 % .1344 % .0035 % .0030 % .2161 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0251 .0204 1.0000 .8524 .9052 8.2758 .6830 .0220 .0163 .3871 .0101 .0086 .6225 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT 4578 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 410 ANIMAL NUMBER: C92357 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 8:47 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 605.8 GRAMS PROSECTOR: TODD ACKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: SINGLE BLACK FOCUS, 3 MM IN DIAMETER LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE UTERUS (UT) : -DILATATION,-SLIGHT, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4579 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 411 ANIMAL NUMBER: C92357 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 605.8 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:47 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE BONE, FEMUR (FE),MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC),MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAr /i LIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (MF), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE),MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC),MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4580 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 412 ANIMAL NUMBER: C92358 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 351.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:40 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 84; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY: SINGLE MASS, 20 X 17 X 15 MM, DARK RED, TAN, AND SOFT; CUT SURFACE CONTAINS A TAN FLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 11 X 8 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT LN, MESENTERIC (MS) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT 4581 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 413 ANIMAL NUMBER: C92358 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 351.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:40 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS - SKIN/HAIRCOAT; DARK RED AND DRY ENTIRE HEAD: DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4582 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 414 ANIMAL NUMBER: C92358 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 351.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:40 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) 4583 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 415 ANIMAL NUMBER: C92359 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/13/00 STUDY DAY OF DEATH: 648 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/13/00 8:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 79; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 92; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 40 X 32 X 25 MM, DARK RED, TAN, GRAY, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) -CYST(S); BILATERAL PECTORAL REGIONS AND PERINEAL REGION: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 15 MM IN DIAMETER (CAP 19) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 20 X 19 X 10 MM, DARK RED, TAN, GRAY, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT 4584 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 416 ANIMAL NUMBER: C92359 SEX: FEMALE DATE OF DEATH: 01/13/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/13/00 8:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 648 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 9 X 8 X 6 MM, DARK RED, TAN, AND SOFT UTERUS (UT) : -CYST(S); LEFT, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 8 X 5 X 5 MM OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 7 MM IN DIAMETER HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : >AUTOLYTIC ILEUM (IL) : >AUTOLYTIC UTERUS (UT) : -DILATATION,-MODERATE, FOCAL OVARY (OV) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,SLIGHT, MULTI-FOCAL 4585 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 417 ANIMAL NUMBER: C92359 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/13/00 STUDY DAY OF DEATH: 648 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/13/00 8:23 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY -ANIMAL THIN LN, MEDIASTINAL (ML) : >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) 4586 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 418 ANIMAL NUMBER: C92360 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 11:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.2 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 61; 80 X 65MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 92; 45 X 45MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 96; 60 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BLUE SKIN; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 3 X 2 MM; COLLECTED ON ROUTINE SECTIONS SKIN (SK) : >NOTE:>SKIN TAKEN FROM RIGHT SIDE DUE TO MASS ON LEFT SIDE MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION, EXTENDS TO MID PERIANAL REGION: SINGLE MASS, 90 X 60 X 20 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; SOME AREAS EXUDE A REDDISH-BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 50 X 40 X 35 MM, RED, DARK RED, TAN AND SOFT; SOME SECTIONS EXUDE A REDDISH-BROWN SEMIFLUID (CAP 23); REGIONAL LN (SEE AXILLARY) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -FIBROSIS,-SEVERE, FOCAL -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,- 2 NEOPLASMS PRESENT 4587 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 419 ANIMAL NUMBER: C92360 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 11:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.2 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; RIGHT AXILLARY REGION: SINGLE MASS, 50 X 40 X 25 MM, RED, TAN AND SOFT; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID (CAP 25); REGIONAL LN (SEE AXILLARY) >NOTE:>MAMMARY TAKEN FROM RIGHT SIDE DUE TO MASS ON LEFT SIDE PITUITARY (PI) : -MASS(ES); 8 X 6 X 3 MM, RED, DARK RED, TAN AND SOFT UTERUS (UT) : -CYST(S); RIGHT, WITHIN HORN: SINGLE CLEAR FLUID FILLED CYST, 10 X 5 X 5 MM OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, FOCAL OVARY (OV) : -CYST,-PRESENT 4588 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 420 ANIMAL NUMBER: C92360 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 11:24 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.2 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; BILATERAL: LEFT IS 12 X 6 X 3 MM, RIGHT IS 10 X 5 X 2 MM (CAP 24) -MOTTLED; LEFT: RED AND TAN FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); BILATERAL HINDFEET: SINGLE TAN, DRY CRUSTED AREA ON EACH, EACH IS 3 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : >UNREMARKABLE FOOT/FOOTPAD (FP) : -EROSION,-SLIGHT, FOCAL -FIBROSIS,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL 4589 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 421 ANIMAL NUMBER: C92360 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 585.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:24 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL),LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE,SCIATIC(SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4590 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 422 ANIMAL NUMBER: C92361 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 13:36 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 73; 110 X 100MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 80; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 55 X 45 X 35 MM (39.6 GRAMS), TAN, SOFT AND LOBULATED; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID MATERIAL; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -THICKENED; VENTRAL THORACIC REGION: TAN AND FIRM (CAP 23) HISTOPATHOLOGY LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT 4591 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 423 ANIMAL NUMBER: C92361 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 13:36 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; LEFT VENTRAL ABDOMINAL REGION, EXTENDING TO BILATERAL INGUINAL REGIONS: SINGLE MASS, 125 X 105 X 60 MM (297.2 GRAMS), TAN, RED, FIRM, VASCULAR, AND LOBULATED; SAME ON CUT SECTION; PORTIONS COLLECTED; OVERLYING SKIN HAS A SINGLE BROWN DRY ULCERATED AREA, 30 MM IN DIAMETER (CAP 24); REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: EACH IS 7 X 6 X 5 MM -MOTTLED; BILATERAL: DARK BROWN AND TAN PITUITARY (PI) : -MASS(ES); 7 X 5 X 4 MM, TAN, DARK RED, SOFT, AND LOBULATED LN, AXILLARY (AX) : -LARGE; BILATERAL: UP TO 10 X 6 X 3 MM (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL 4592 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 424 ANIMAL NUMBER: C92361 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/07/00 STUDY DAY OF DEATH: 702 STUDY WEEK OF DEATH: 101 TERMINAL BODY WEIGHT: 664.1 GRAMS DATE AND TIME OF NECROPSY: 03/07/00 13:36 PROSECTOR: DAVID SCHUETTE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4593 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 425 ANIMAL NUMBER: C92362 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 102; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES, DIFFUSE: MULTIPLE TAN AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTIONS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 35 X 28 X 25 MM, RED, DARK RED, TAN, AND FIRM; ON SECTION EXUDES DARK BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL LIVER (LI) : -FIBROSIS,-SEVERE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL -NECROSIS, HEPATOCELLULAR,-SEVERE, MULTI-FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT PARATHYROID (PT) : -HYPERPLASIA,-MINIMAL, DIFFUSE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT 4594 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 426 ANIMAL NUMBER: C92362 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HEART (HT) : -LIGHT FOCUS(I)/AREA(S); AT APEX: SINGLE LIGHT AREA, 4 MM IN DIAMETER ADRENAL, CORTEX (AC) : -MASS(ES); RIGHT, INVOLVING ENTIRE ORGAN: SINGLE MASS, RED, DARK RED, TAN, AND FIRM, 10 X 8 X 8 MM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (RENAL) NOT IDENTIFIED PITUITARY (PI) : -MASS(ES); 10 X 6 X 6 MM, RED, DARK RED, TAN AND SOFT OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL OVARY (OV) : >UNREMARKABLE DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4595 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 427 ANIMAL NUMBER: C92362 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 321.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:45 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SB), 4596 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 428 ANIMAL NUMBER: C92363 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 11:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 573.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 76; 80 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION EXTENDING TO LEFT AXILLARY REGION: SINGLE MASS, 70 X 55 X 45 MM, RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE MANDIBULAR) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 7 X 6 MM, DARK RED, FIRM AND LOBULATED UTERUS (UT) : -SMALL; BILATERAL: EACH IS 2 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL SKIN (SK) : -B-FIBROMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL >NOTE:>MAMMARY MASS DESCRIBED GROSSLY WAS A SUBCUTANEOUS FIBROMA BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : >UNREMARKABLE OVARY (OV) : >UNREMARKABLE 4597 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 429 ANIMAL NUMBER: C92363 SEX: FEMALE DATE OF DEATH: 11/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/01/99 11:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 573.9 GRAMS PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND TAN (CAP 18) LN, AXILLARY (AX) : -DIFFUSELY RED; LEFT: DARK RED (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED, BROWN AND DRY MATERIAL -DISCHARGE-NASAL; DARK RED AND DRY MATERIAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL LN, AXILLARY (AX) : >UNREMARKABLE 4598 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 430 ANIMAL NUMBER: C92363 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/01/99 STUDY DAY OF DEATH: 575 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 573.9 GRAMS DATE AND TIME OF NECROPSY: 11/01/99 11:38 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), FOOT/FOOTPAD (FP),LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEBGL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4599 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 431 ANIMAL NUMBER: C92364 SEX: FEMALE DATE OF DEATH: 06/17/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/17/99 15:13 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 206.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; ROUGH HAIR COAT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; CIRCLING; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 9 X 8 MM, RED, TAN AND FIRM GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; MATERIAL -ANIMAL THIN : LEFT: DRY, RED HISTOPATHOLOGY KIDNEY (KD) : -HEMORRHAGE,-SLIGHT, FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4600 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 432 ANIMAL NUMBER: C92364 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/17/99 STUDY DAY OF DEATH: 438 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 206.9 GRAMS DATE AND TIME OF NECROPSY: 06/17/99 15:13 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4601 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 433 ANIMAL NUMBER: C92365 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 8:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 388.4 GRAMS PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0416 .0382 2.1778 1.2772 1.3060 9.9235 1.7345 .0670 .0635 .5717 .0145 .0137 .9764 .0107 % .0098 % .5607 % .3288 % .3363 % 2.5550 % .4466 % .0173 % .0163 % .1472 % .0037 % .0035 % .2514 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0191 .0175 1.0000 .5865 .5997 4.5567 .7964 .0308 .0292 .2625 .0067 .0063 .4483 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4602 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 434 ANIMAL NUMBER: C92365 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 388.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:43 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4603 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 435 ANIMAL NUMBER: C92366 SEX: FEMALE DATE OF DEATH: 04/28/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/28/98 8:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 215.3 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 9.3217 -------------------------------------------------- ------------------------------------ 4.3296 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4604 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 436 ANIMAL NUMBER: C92366 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 215.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:59 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4605 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 437 ANIMAL NUMBER: C92367 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:47 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 X 4 MM; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -HYPERPLASIA, BILE DUCT,-SLIGHT, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -PARASITISM,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4606 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 438 ANIMAL NUMBER: C92367 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 468.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:47 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4607 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 439 ANIMAL NUMBER: C92368 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 431.0 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:26 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 91; 35 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 100; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERINEAL REGION: SINGLE MASS, 40 X 40 X 20 MM, TAN, RED, GRAY AND SOFT; CUT SURFACE EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 40 X 30 X 15 MM, TAN, RED AND SOFT; CUT SURFACE EXUDES A TAN, CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -FIBROSIS,-SLIGHT, FOCAL -HYPERPLASIA, C-CELL,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4608 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 440 ANIMAL NUMBER: C92368 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 431.0 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:26 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); ATTACHED TO LEFT POSTERIOR ASPECT: SINGLE MASS, 6 MM IN DIAMETER, DARK RED, RED AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4609 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 441 ANIMAL NUMBER: C92368 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/17/00 STUDY DAY OF DEATH: 712 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 431.0 GRAMS DATE AND TIME OF NECROPSY: 03/17/00 8:26 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) 4610 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 442 ANIMAL NUMBER: C92369 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.0 GRAMS PROSECTOR: JARED FISHEL RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 51; 115 X 110MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 102; 25 X 20 MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: APPEAR SWOLLEN SKIN (SK) : >NOTE:>SKIN TAKEN FROM THE RIGHT SIDE DUE TO MASS ON THE LEFT SIDE MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 25 MM IN DIAMETER, DARK RED AND FIRM; ON SECTION EXUDES A DARK RED FLUID (CAP 21); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT ABDOMINAL REGION, EXTENDS TO LEFT INGUINAL REGION: SINGLE MASS, 110 X 100 X 40 MM (204.0 GRAMS), RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL LIVER (LI) : >UNREMARKABLE THYROID (TY) : -B-ADENOMA, C-CELL,- 2 NEOPLASMS PRESENT SKIN (SK) : -FIBROSIS,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 4611 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 443 ANIMAL NUMBER: C92369 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.0 GRAMS PROSECTOR: JARED FISHEL RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : >NOTE:>MAMMARY TAKEN FROM THE RIGHT SIDE DUE TO MASS ON THE LEFT SIDE SPLEEN (SP) : -LARGE; 60 X 18 X 15 MM ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 X 8 X 8 MM (LEFT IS 4 X 3 X 2 MM) PITUITARY (PI) : -RED FOCUS(I)/AREA(S); RIGHT POSTERIOR ASPECT: SINGLE DARK RED AREA, 2 MM IN DIAMETER OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 7 MM IN DIAMETER HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -VACUOLATION,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND -INFLAMMATION, FOCAL OVARY (OV) : -CYST,-PRESENT (HG) : CHRONIC,-MINIMAL, MULTI DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4612 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 444 ANIMAL NUMBER: C92369 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.0 GRAMS PROSECTOR: JARED FISHEL RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, MEDIASTINAL (ML) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE 4613 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 445 ANIMAL NUMBER: C92369 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 597.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:59 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SG), 4614 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 446 ANIMAL NUMBER: C92370 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 572.0 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 8:06 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 80; 55 X 55MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC; WEEK 80; 45 X 35MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 100; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -MOTTLED; BILATERAL: TAN AND BROWN LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE AREAS, UP TO 3 MM IN DIAMETER; COLLECTED WITH ROUTINE SECTIONS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 12 X 21 X 35 MM, SOFT, TAN, DARK BROWN AND LOBULATED; ON SECTION CONTAINS A DARK BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 40 X 55 X 68 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; OVERLYING SKIN HAS A DARK BROWN AND RED, DRY ABRADED AREA, 40 X 50 MM; COLLECTED WITH MASS; REGIONAL LN (SEE AXILLARY) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 4615 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 447 ANIMAL NUMBER: C92370 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 572.0 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 8:06 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; RIGHT CERVICAL REGION, EXTENDS TO MID VENTRAL THORACIC REGION: SINGLE MASS, 35 X 43 X 52 MM, FIRM, RED, TAN AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -CYST(S); RIGHT ASPECT: SINGLE CYST, 4 MM IN DIAMETER, FILLED WITH A DARK RED FLUID LN, AXILLARY (AX) : -LARGE; RIGHT: 4 X 8 X 10 MM 3 X 4 X 7 MM) (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN (LEFT IS BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-SLIGHT, MULTI FOCAL -FIBROSIS,-MINIMAL, MULTI-FOCAL THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, FOCAL -HEMORRHAGE,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-MINIMAL, MULTI-FOCAL 4616 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 448 ANIMAL NUMBER: C92370 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/02/00 STUDY DAY OF DEATH: 697 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 572.0 GRAMS DATE AND TIME OF NECROPSY: 03/02/00 8:06 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4617 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 449 ANIMAL NUMBER: C92371 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 X 7 X 6 MM, RED, DARK RED AND SOFT HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MINIMAL, MULTI-FOCAL ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT DUODENUM (DU) : >AUTOLYTIC 4618 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 450 ANIMAL NUMBER: C92371 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); LEFT HORN, WITHIN WALL: SINGLE CLEAR FLUID FILLED CYST, 4 X 3 X 3 MM GENERAL COMMENT (GC) : -ANIMAL OBESE ILEUM (IL) : >AUTOLYTIC UTERUS (UT) : -DILATATION,-MODERATE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4619 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 451 ANIMAL NUMBER: C92371 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 14:38 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4620 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 452 ANIMAL NUMBER: C92372 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 411.9 GRAMS PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0426 .0380 2.1520 1.4414 1.4720 11.1932 1.7408 .0816 .0713 .6446 .0146 .0172 .9058 .0103 % .0092 % .5225 % .3499 % .3574 % 2.7175 % .4226 % .0198 % .0173 % .1565 % .0035 % .0042 % .2199 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0198 .0177 1.0000 .6698 .6840 5.2013 .8089 .0379 .0331 .2995 .0068 .0080 .4209 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 X 3 X 2 MM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -LARGE; 7 X 6 X 4 MM STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: TWO BROWN AREAS, EACH IS 3 X 2 MM MAMMARY, FEMALE (MF) : >UNREMARKABLE LN, MESENTERIC (MS) : -EDEMA,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : >UNREMARKABLE STOMACH, GL (ST) : -EDEMA,-MODERATELY SEVERE, FOCAL 4621 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 453 ANIMAL NUMBER: C92372 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 411.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4622 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 454 ANIMAL NUMBER: C92373 SEX: FEMALE DATE OF DEATH: 04/28/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/28/98 8:29 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.3 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.2075 -------------------------------------------------- ------------------------------------ 3.8481 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4623 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 455 ANIMAL NUMBER: C92373 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 187.3 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:29 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4624 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 456 ANIMAL NUMBER: C92374 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 9:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.3 GRAMS PROSECTOR: JARED FISHEL RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -MINERALIZATION, TUBULAR,-MINIMAL, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, FOCAL -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT EYE (EY) : -INFLAMMATION, ACUTE, CORNEAL,-MINIMAL, MULTI-FOCAL -VASCULARIZATION, CORNEAL,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 4625 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 457 ANIMAL NUMBER: C92374 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:41 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 8 X 6 X 6 MM, GRAY, RED AND SOFT LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: DARK RED (CAP 22) LN, POPLITEAL (PP) : -LARGE; LEFT: 9 MM IN DIAMETER (RIGHT NOT IDENTIFIED) -MOTTLED; LEFT: RED AND TAN (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EROSION,-MINIMAL, FOCAL CERVIX (CV) : -B-LEIOMYOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL ADIPOSE TISSUE (AT) : -M-LIPOSARCOMA,-NEOPLASM PRESENT LN, POPLITEAL (PP) : >TISSUE MISSING >NOTE:>POPLITEAL LYMPH NODE DESCRIBED GROSSLY WAS A SUBCUTANEOUS LIPOSARCOMA 4626 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 458 ANIMAL NUMBER: C92374 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 572.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:41 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4627 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 459 ANIMAL NUMBER: C92375 SEX: FEMALE DATE OF DEATH: 12/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/01/99 12:58 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 427.1 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: BROWN AND TAN BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 8 X 8 MM, RED, DARK RED, TAN, AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL PARATHYROID (PT) : >SECTION EXAMINED; TISSUE NOT PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT THYMUS (TH) : -CYST,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL 4628 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 460 ANIMAL NUMBER: C92375 SEX: FEMALE DATE OF DEATH: 12/01/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/01/99 12:58 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 427.1 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4629 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 461 ANIMAL NUMBER: C92375 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/01/99 STUDY DAY OF DEATH: 605 STUDY WEEK OF DEATH: 87 TERMINAL BODY WEIGHT: 427.1 GRAMS DATE AND TIME OF NECROPSY: 12/01/99 12:58 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4630 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 462 ANIMAL NUMBER: C92376 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 321.1 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0430 .0500 2.1321 1.2378 1.2613 9.9801 1.5665 .0449 .0412 .5194 .0119 .0097 1.2052 .0134 % .0156 % .6640 % .3855 % .3928 % 3.1081 % .4879 % .0140 % .0128 % .1618 % .0037 % .0030 % .3753 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0202 .0235 1.0000 .5806 .5916 4.6809 .7347 .0211 .0193 .2436 .0056 .0045 .5653 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >MASS(ES); VENTRAL-CERVICAL; WEEK 46; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 45 X 30 X 25 MM, RED, TAN, GRAY AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL 4631 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 463 ANIMAL NUMBER: C92376 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:31 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 321.1 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -DIFFUSELY RED; LEFT; RIGHT IS NORMAL (CAP 18) GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, VACUOLATED,MINIMAL, MULTI-FOCAL 4632 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 464 ANIMAL NUMBER: C92376 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 321.1 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:31 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4633 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 465 ANIMAL NUMBER: C92377 SEX: FEMALE DATE OF DEATH: 05/03/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/03/99 11:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: JILL PAUS RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 31; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); VENTRAL CERVICAL REGION: SINGLE MASS, 30 X 17 X 15 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE (CAP 18); REGIONAL LN (MANDIBULAR) NORMAL (CAP 19) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 5 MM, RED, DARK RED, TAN AND SOFT GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; RED, DRY MATERIAL -DISCHARGE-NASAL; RED, DRY MATERIAL HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT ILEUM (IL) : >AUTOLYTIC OVARY (OV) : -INFLAMMATION, CHRONIC,-MODERATE, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT MULTI 4634 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 466 ANIMAL NUMBER: C92377 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 05/03/99 11:06 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) 4635 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 467 ANIMAL NUMBER: C92378 SEX: FEMALE DATE OF DEATH: 08/26/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/26/99 7:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 292.2 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TAN; HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-SLIGHT, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4636 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 468 ANIMAL NUMBER: C92378 SEX: FEMALE DATE OF DEATH: 08/26/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/26/99 7:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 292.2 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 7 X 5 MM, RED, DARK RED AND TAN STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, NEAR JUNCTION OF GLANDULAR MUCOSA: SINGLE TAN ERODED AREA, 5 MM IN DIAMETER OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK BROWN AND DRY MATERIAL -DISCHARGE-NASAL; DARK BROWN AND DRY MATERIAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, DIFFUSE -EROSION,-SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4637 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 469 ANIMAL NUMBER: C92378 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/26/99 STUDY DAY OF DEATH: 508 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 292.2 GRAMS DATE AND TIME OF NECROPSY: 08/26/99 7:45 PROSECTOR: DAVID SCHUETTE RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4638 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 470 ANIMAL NUMBER: C92379 SEX: FEMALE DATE OF DEATH: 08/16/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/16/99 10:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 49; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-THORACIC; WEEK 70; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY SKIN (SK) : >NOTE:>COLLECTED FROM RIGHT SIDE DUE TO MAMMARY GLAND MASS MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 29 X 19 X 11 MM, RED, DARK RED, TAN, LOBULATED AND VASCULAR; SOME SECTIONS EXUDE A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -CYST(S); RIGHT AXILLARY REGION: SINGLE TAN FLUID FILLED CYST, 12 MM IN DIAMETER; COLLECTED; POSSIBLY CONSISTENT WITH MASS (RIGHT AXILLARY) AS NOTED IN CLINICAL OBSERVATIONS -MASS #2; RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 13 X 9 X 4 MM, TAN, RED AND LOBULATED; ON SECTION EXUDES A TAN CASEOUS SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 18) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-ADENOMA,- 2 NEOPLASMS PRESENT 4639 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 471 ANIMAL NUMBER: C92379 SEX: FEMALE DATE OF DEATH: 08/16/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/16/99 10:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : >NOTE:>COLLECTED FROM RIGHT SIDE DUE TO MAMMARY GLAND MASS EYE (EY) : >NOTE:>LEFT EYE RUPTURED AT NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 10 X 6 MM, RED, DARK RED, TAN AND SOFT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4640 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 472 ANIMAL NUMBER: C92379 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 08/16/99 STUDY DAY OF DEATH: 498 STUDY WEEK OF DEATH: 72 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 08/16/99 10:41 PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) (SM), (UT), 4641 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 473 ANIMAL NUMBER: C92380 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 338.1 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 10:55 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 60; 45 X 45MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 45 X 40 X 20 MM, TAN, FIRM, AND VASCULAR; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE YELLOW CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION LEFT AXILLARY REGION: SINGLE BROWN SEMIFLUID FILLED CYST, 15 MM IN DIAMETER; COLLECTED (CAP 19) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 20 X 20 X 8 MM, TAN, LOBULATED, AND SOFT; ON SECTION, EXUDES A LIGHT GREEN SEMISOLID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT 4642 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 474 ANIMAL NUMBER: C92380 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 338.1 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 10:55 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 13 X 12 X 8 MM, DARK RED, SOFT, AND CYSTIC VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: YELLOW CASEOUS MATERIAL LN, MANDIBULAR (MN) : -LARGE; BILATERAL: UP TO 10 X 7 X 3 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL: LOWER INCISORS AND LEFT UPPER INCISOR -ANIMAL THIN -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: BROKEN OFF AT BASE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-SLIGHT, MULTI FOCAL VAGINA (VA) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-MODERATE, DIFFUSE 4643 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 475 ANIMAL NUMBER: C92380 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/18/99 STUDY DAY OF DEATH: 561 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 338.1 GRAMS DATE AND TIME OF NECROPSY: 10/18/99 10:55 PROSECTOR: JARED FISHEL RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) 4644 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 476 ANIMAL NUMBER: C92381 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 299.5 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 8:10 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 5 X 3 MM, RED, TAN, AND SOFT STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE RED, DARK GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -HEMORRHAGE,-MINIMAL, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, FOCAL -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4645 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 477 ANIMAL NUMBER: C92381 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/29/99 STUDY DAY OF DEATH: 480 STUDY WEEK OF DEATH: 69 TERMINAL BODY WEIGHT: 299.5 GRAMS DATE AND TIME OF NECROPSY: 07/29/99 8:10 PROSECTOR: JILL PAUS RECORDER: TONY HANFELD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: RED DRY MATERIAL -DISCHARGE-NASAL; RED DRY MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (MF), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4646 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 478 ANIMAL NUMBER: C92382 SEX: FEMALE DATE OF DEATH: 05/03/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/03/99 11:13 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 231.0 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; GENERALIZED; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); LEFT LATERAL LOBE: SINGLE DARK RED AREA, 5 X 2 MM (COLLECTED ON ROUTINE SECTION) MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (COLLECTED WITH ROUTINE SECTION) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 9 MM IN DIAMETER (FOR COMPARISON RIGHT IS 5 MM IN DIAMETER) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 8 X 6 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT SPLEEN (SP) : -PIGMENT, INCREASED,-MODERATE, MULTI FOCAL THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -THROMBUS,-SEVERE, FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4647 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 479 ANIMAL NUMBER: C92382 SEX: FEMALE DATE OF DEATH: 05/03/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 05/03/99 11:13 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 231.0 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 4 X 2 MM STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA, ALONG GREATER CURVATURE: MULTIPLE TAN ERODED AREAS, UP TO 5 X 2 MM URINARY BLADDER (UB) : -CALCULUS(I); WITHIN LUMEN: MULTIPLE HARD TAN CALCULI, UP TO 20 X 10 X 10 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; BROWN AND MOIST -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY CRUSTED MATERIAL -ANIMAL THIN STOMACH, GL (ST) : -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL STOMACH, NONGL (SU) : -EDEMA,-MODERATE, DIFFUSE -EROSION,-MODERATELY SEVERE, FOCAL URINARY BLADDER (UB) : -HYPERPLASIA, TRANSITIONAL CELL,MODERATE, DIFFUSE -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4648 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 480 ANIMAL NUMBER: C92382 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 05/03/99 STUDY DAY OF DEATH: 393 STUDY WEEK OF DEATH: 57 TERMINAL BODY WEIGHT: 231.0 GRAMS DATE AND TIME OF NECROPSY: 05/03/99 11:13 PROSECTOR: MICHELE MARGGI RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC HEART (HT), LN, MESENTERIC (MS), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), UTERUS OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SN), (UT), 4649 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 481 ANIMAL NUMBER: C92383 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:33 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 96; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADCRANIAL; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 35 X 30 X 7 MM, RED, FIRM AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) TAN, HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL LIVER (LI) : -CYST, BILIARY,-MODERATE, FOCAL -DEGENERATION, CYSTIC,-MODERATE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -INFLAMMATION, CHRONIC,-SLIGHT, FOCAL 4650 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 482 ANIMAL NUMBER: C92383 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:33 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -MASS(ES); INVOLVING ENTIRE LEFT ADRENAL: SINGLE MASS, 17 MM IN DIAMETER, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; REGIONAL LN (RENAL) NOT IDENTIFIED JEJUNUM (JE) : -DIVERTICULUM(A); MID JEJUNUM: SINGLE DIVERTICULUM, 8 X 5 X 3 MM; COLLECTED ON ROUTINE SECTION UTERUS (UT) : -MASS(ES); WITHIN WALL OF RIGHT HORN: SINGLE MASS, 20 X 15 X 10 MM, RED, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER -MASS(ES); RIGHT: SINGLE MASS, 30 X 25 X 20 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 24) THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT JEJUNUM (JE) : ^UNREMARKABLE UTERUS (UT) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT -B-GRANULOSA/THECA CELL TUMOR,-NEOPLASM PRESENT 4651 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 483 ANIMAL NUMBER: C92383 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:33 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT CAVITY, ABDOM (PC) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT 4652 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 484 ANIMAL NUMBER: C92383 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 554.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:33 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), LN, LUMBAR/ILIAC (LM) 4653 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 485 ANIMAL NUMBER: C92384 SEX: FEMALE DATE OF DEATH: 12/10/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/10/99 9:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 320.7 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE AREAS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION PANCREAS (PA) : -MASS(ES); ALONG SPLEEN: SINGLE MASS, 10 X 10 X 6 MM, TAN, RED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (PANCREATIC) NOT IDENTIFIED MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: AND SOFT BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) TAN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LIVER (LI) : -TELANGIECTASIS,-MINIMAL, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT PANCREAS (PA) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT 4654 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 486 ANIMAL NUMBER: C92384 SEX: FEMALE DATE OF DEATH: 12/10/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 12/10/99 9:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 320.7 GRAMS PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED, GRAY AND SOFT STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE -EROSION/ULCERATION; MUCOSA: MULTIPLE BROWN AND TAN ULCERATED AREAS, UP TO 6 X 5 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; PERINEUM: MOIST DARK BROWN MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY DARK BROWN MATERIAL -ANIMAL THIN -GI TRACT-DARK FLUID CONTENT; ILEUM AND CECUM: CONTAINS A DARK BROWN SEMIFLUID MATERIAL THYMUS (TH) : -CYST,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATELY SEVERE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -ULCERATION,-SEVERE, MULTI-FOCAL UTERUS (UT) : -DILATATION,-MINIMAL, FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4655 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 487 ANIMAL NUMBER: C92384 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/10/99 STUDY DAY OF DEATH: 614 STUDY WEEK OF DEATH: 88 TERMINAL BODY WEIGHT: 320.7 GRAMS DATE AND TIME OF NECROPSY: 12/10/99 9:48 PROSECTOR: DAVID SCHUETTE RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4656 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 488 ANIMAL NUMBER: C92385 SEX: FEMALE DATE OF DEATH: 01/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/31/00 13:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 334.6 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 14 X 12 X 10 MM, RED, DARK RED, TAN, BROWN, AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -INFARCT,-SLIGHT, FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -HEMORRHAGE,-SLIGHT, FOCAL -VACUOLATION, HEPATOCELLULAR,-SEVERE, DIFFUSE THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4657 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 489 ANIMAL NUMBER: C92385 SEX: FEMALE DATE OF DEATH: 01/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/31/00 13:35 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 334.6 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); WITHIN WALL OF RIGHT HORN AT BIFURCATION: SINGLE DARK RED FLUID FILLED CYST, 4 MM IN DIAMETER VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN, MUCOID SEMIFLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN UTERUS (UT) : >UNREMARKABLE VAGINA (VA) : ^UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4658 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 490 ANIMAL NUMBER: C92385 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 334.6 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 13:35 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), HEMATO NEOPLASIA (HN) 4659 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 491 ANIMAL NUMBER: C92386 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 308.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:14 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 80; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); EAR-LEFT; WEEK 92; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 95; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 101; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -CRUSTED AREA(S); WITHIN LEFT PINNA: SINGLE TAN CRUSTED AREA, 5 X 3 MM; COLLECTED INTACT MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 23 X 15 X 15 MM, BROWN, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A TAN, MOIST, ULCERATED AREA, 1 MM IN DIAMETER; COLLECTED INTACT WITH MASS (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 23); POSSIBLY CONSISTENT WITH MASS (LEFT CERVICAL ABDOMINAL REGION) NOTED IN CLINICAL OBSERVATIONS HISTOPATHOLOGY LUNG (LU) : -INFLAMMATION, GRANULOMATOUS,-SLIGHT, MULTI-FOCAL KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT SKIN (SK) : -B-ADENOMA, SEBACEOUS GLAND,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL -MINERALIZATION,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 4660 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 492 ANIMAL NUMBER: C92386 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 308.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:14 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 53 X 41 X 24 MM, TAN, RED, LOBULATED, AND FIRM; SOME SECTIONS EXUDE A TAN AND GREEN SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASSES (VENTRAL ABDOMINAL AND LEFT INGUINAL REGIONS) AS NOTED IN CLINICAL OBSERVATIONS PITUITARY (PI) : -LARGE; 6 X 5 X 4 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-MODERATE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4661 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 493 ANIMAL NUMBER: C92386 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 308.0 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 13:14 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) 4662 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 494 ANIMAL NUMBER: C92387 SEX: FEMALE DATE OF DEATH: 08/31/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/31/99 14:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 341.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 59; 45 X 30MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 43 X 25 X 20 MM, TAN AND FIRM; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL 4663 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 495 ANIMAL NUMBER: C92387 SEX: FEMALE DATE OF DEATH: 08/31/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/31/99 14:41 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 341.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, DARK RED AND FIRM STOMACH, GL (ST) : -RED FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK RED AREAS, UP TO 2 X 1 MM STOMACH, NONGL (SU) : -THICKENED WALL; ALL REGIONS, DIFFUSE -EROSION/ULCERATION; MUCOSA: SINGLE DARK RED ERODED AREA, 10 X 5 MM UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 15 X 5 X 5 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MATERIAL -ANIMAL THIN DRY RED ADRENAL, CORTEX (AC) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE STOMACH, NONGL (SU) : -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL -ULCERATION,-SEVERE, FOCAL UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, DIFFUSE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MEDIASTINAL (ML) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, MULTI-FOCAL >NOTE:>MEDIASTINAL LYMPH NODE WAS PRESENT ON THYMUS SLIDE 4664 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 496 ANIMAL NUMBER: C92387 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/31/99 STUDY DAY OF DEATH: 513 STUDY WEEK OF DEATH: 74 TERMINAL BODY WEIGHT: 341.8 GRAMS DATE AND TIME OF NECROPSY: 08/31/99 14:41 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS - SKIN/HAIRCOAT; ENTIRE ABDOMINAL REGION: DRY RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), HEMATO NEOPLASIA (HN) 4665 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 497 ANIMAL NUMBER: C92388 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 8:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 436.9 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0614 .0410 2.1627 1.3578 1.3404 11.3882 1.6427 .0493 .0437 .5916 .0145 .0155 1.0659 .0141 % .0094 % .4950 % .3108 % .3068 % 2.6066 % .3760 % .0113 % .0100 % .1354 % .0033 % .0035 % .2440 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0284 .0190 1.0000 .6278 .6198 5.2657 .7596 .0228 .0202 .2735 .0067 .0072 .4929 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LUNG (LU) : -HEMORRHAGE,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 4666 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 498 ANIMAL NUMBER: C92388 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 8:45 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 436.9 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4667 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 499 ANIMAL NUMBER: C92388 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 436.9 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:45 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (FM), 4668 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 500 ANIMAL NUMBER: C92389 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:45 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .1123 .0474 2.0204 1.3505 1.4494 9.5787 2.2838 .0660 .0684 .4422 .0103 .0114 1.2955 .0315 % .0133 % .5667 % .3788 % .4066 % 2.6869 % .6406 % .0185 % .0192 % .1240 % .0029 % .0032 % .3634 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0556 .0235 1.0000 .6684 .7174 4.7410 1.1304 .0327 .0339 .2189 .0051 .0056 .6412 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE WHITE AREAS, UP TO 3 MM IN DIAMETER ADRENAL, CORTEX (AC) : -LARGE; LEFT: 10 X 8 X 7 MM (FOR COMPARISON RIGHT IS 5 X 3 X 3 MM) LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE, MULTI-FOCAL PANCREAS (PA) : >TISSUE MISSING ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL RECTUM (RE) : -PARASITISM,-PRESENT 4669 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 501 ANIMAL NUMBER: C92389 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 356.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:45 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), LIVER (LI), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (SG), 4670 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 502 ANIMAL NUMBER: C92390 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0368 .0211 2.0064 1.1624 1.0052 9.1787 1.5087 .0798 .0865 .6107 .0175 .0151 .6848 .0108 % .0062 % .5880 % .3407 % .2946 % 2.6901 % .4422 % .0234 % .0254 % .1790 % .0051 % .0044 % .2007 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0183 .0105 1.0000 .5793 .5010 4.5747 .7519 .0398 .0431 .3044 .0087 .0075 .3413 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE RED AREAS, UP TO 2 X 1 MM; COLLECTED ON ROUTINE SECTIONS PITUITARY (PI) : -LARGE; 6 X 4 X 3 MM; APPEARS SWOLLEN KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LIVER (LI) : -HEMORRHAGE,-SLIGHT, FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, FOCAL ADRENAL, CORTEX (AC) : -ONE EXAMINED,-PRESENT ADRENAL, MEDULLA (MA) : -ONE EXAMINED,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL 4671 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 503 ANIMAL NUMBER: C92390 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.2 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); DIFFUSE: MULTIPLE DARK RED AREAS, UP TO 1 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4672 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 504 ANIMAL NUMBER: C92390 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 341.2 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:39 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4673 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 505 ANIMAL NUMBER: C92391 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 61; 20 X 30MM; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 84; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 84; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES, UP TO 1 MM IN DIAMETER MAMMARY, FEMALE (MF) : -MASS(ES); LEFT VENTRAL CERVICAL REGION: SINGLE MASS, 35 X 25 X 15 MM, TAN, GRAY, AND FIRM; CUT SURFACE CONTAINS A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 18) HISTOPATHOLOGY LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,MODERATE, FOCAL -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -B-FIBROMA,-NEOPLASM PRESENT 4674 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 506 ANIMAL NUMBER: C92391 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 38 X 25 X 15 MM, DARK RED, TAN, AND SOFT; CUT SURFACE CONTAINS A TAN CASEOUS SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 19) -MASS #3; RIGHT ABDOMINAL REGION: SINGLE MASS, 24 MM IN DIAMETER, DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NORMAL (CAP 19) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 10 X 7 MM, DARK RED, TAN, AND SOFT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT HEART (HT) : -FIBROSIS,-SLIGHT, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-SLIGHT, FOCAL -THROMBUS,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -PIGMENT,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4675 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 507 ANIMAL NUMBER: C92391 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DARK RED AND MOIST ILEUM (IL) : >AUTOLYTIC DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4676 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 508 ANIMAL NUMBER: C92391 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 01/12/00 STUDY DAY OF DEATH: 647 STUDY WEEK OF DEATH: 93 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 01/12/00 10:36 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, INGUINAL (IN), HEMATO NEOPLASIA (HN) 4677 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 509 ANIMAL NUMBER: C92392 SEX: FEMALE DATE OF DEATH: 06/21/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/21/99 12:39 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 286.8 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 4 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MODERATE, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-MODERATE, FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MODERATE, DIFFUSE OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4678 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 510 ANIMAL NUMBER: C92392 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/21/99 STUDY DAY OF DEATH: 442 STUDY WEEK OF DEATH: 64 TERMINAL BODY WEIGHT: 286.8 GRAMS DATE AND TIME OF NECROPSY: 06/21/99 12:39 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -DISCHARGE-NASAL; DRY RED MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4679 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 511 ANIMAL NUMBER: C92393 SEX: FEMALE DATE OF DEATH: 01/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/18/00 10:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 517.2 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 73; 20 X 25MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 60; 110 X 90MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 92; 15 X 15MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 105 X 75 X 43 MM, TAN, LOBULATED AND FIRM; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (SEE AXILLARY) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 30 X 15 MM, GREEN, TAN, LOBULATED AND SOFT; ON SECTION, EXUDES A GREEN SEMIFLUID; COLLECTED INTACT; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, MULTI-FOCAL LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,MINIMAL, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 4680 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 512 ANIMAL NUMBER: C92393 SEX: FEMALE DATE OF DEATH: 01/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/18/00 10:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 517.2 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT INGUINAL REGION, CRANIAL TO MASS 2: SINGLE MASS, 30 X 23 X 15 MM, TAN, RED AND FIRM; ON SECTION, EXUDES A TAN CREAMY SEMIFLUID (CAP 19); REGIONAL LN (INGUINAL) NOT IDENTIFIED; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL LEFT) NOTED IN CLINICAL OBSERVATIONS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 14 X 8 X 4 MM, DARK RED, RED, GRAY AND FIRM MARROW, FEMUR (FM) : -HYPERCELLULARITY, MARROW,-MODERATE, DIFFUSE MARROW, STERNUM (SE) : -HYPERCELLULARITY, MARROW,-MODERATE, DIFFUSE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT HEART (HT) : -DEGENERATION/NECROSIS,-MINIMAL, MULTI FOCAL -FIBROSIS,-SLIGHT, MULTI-FOCAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4681 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 513 ANIMAL NUMBER: C92393 SEX: FEMALE DATE OF DEATH: 01/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/18/00 10:44 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 517.2 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 6 X 4 X 2 MM; COLLECTED LN, AXILLARY (AX) : -LARGE; BILATERAL: EACH IS 10 X 7 X 3 MM (CAP 18) -DIFFUSELY RED; LEFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL LN, AXILLARY (AX) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL 4682 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 514 ANIMAL NUMBER: C92393 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 517.2 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 10:44 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) 4683 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 515 ANIMAL NUMBER: C92394 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 14:27 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 375.9 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 36; 75 X 50MM; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DEPRESSED FOCUS(I)/AREA(S); CAUDATE LOBE: MULTIPLE DARK BROWN SLIGHTLY DEPRESSED AREAS, UP TO 5 X 4 X 2 MM (CAP 19) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT CERVICAL REGION: SINGLE MASS, 75 X 50 X 35 MM, RED, DARK RED, TAN, LOBULATED, AND VASCULAR; ON SECTION EXUDES A DARK BROWN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 10 X 7 X 6 MM, RED, DARK RED, AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL LIVER (LI) : -CYST, BILIARY,-MODERATE, FOCAL -TELANGIECTASIS,-SEVERE, FOCAL MULTI MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -VACUOLATION,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4684 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 516 ANIMAL NUMBER: C92394 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 375.9 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 14:27 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-NASAL; BILATERAL: DRY, CRUSTED MATERIAL -ANIMAL THIN DARK RED, THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), HEMATO NEOPLASIA (HN) 4685 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 517 ANIMAL NUMBER: C92395 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:01 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0542 .0419 2.5272 1.4087 1.3994 11.0859 1.8104 .0517 .0567 .5787 .0128 .0124 1.0112 .0139 % .0107 % .6472 % .3607 % .3584 % 2.8389 % .4636 % .0132 % .0145 % .1482 % .0033 % .0032 % .2590 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0214 .0166 1.0000 .5574 .5537 4.3866 .7164 .0205 .0224 .2290 .0051 .0049 .4001 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED PITUITARY (PI) : -LARGE; 8 X 6 X 4 MM LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, FOCAL PITUITARY (PI) : >UNREMARKABLE HARDERIAN GLAND (HG) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL RECTUM (RE) : -PARASITISM,-PRESENT 4686 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 518 ANIMAL NUMBER: C92395 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 390.5 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:01 PROSECTOR: WENDY CHERRY RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), SALIV GL, MANDIB (SG), STOMACH, GL STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), HEMATO NEOPLASIA (HN) (ST), 4687 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 519 ANIMAL NUMBER: C92396 SEX: FEMALE DATE OF DEATH: 07/07/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/07/98 8:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.2 GRAMS PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.6550 -------------------------------------------------- ------------------------------------ 2.7816 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4688 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 520 ANIMAL NUMBER: C92396 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 275.2 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:30 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4689 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 521 ANIMAL NUMBER: C92397 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 273.1 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:43 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 8.5809 -------------------------------------------------- ------------------------------------ 3.1420 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >EYES; DISPLACED PUPIL; EYE-RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4690 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 522 ANIMAL NUMBER: C92397 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 273.1 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:43 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4691 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 523 ANIMAL NUMBER: C92398 SEX: FEMALE DATE OF DEATH: 07/07/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/07/98 9:19 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.0 GRAMS PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.7048 -------------------------------------------------- ------------------------------------ 2.6846 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4692 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 524 ANIMAL NUMBER: C92398 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:19 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4693 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 525 ANIMAL NUMBER: C92399 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 242.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:30 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 8.0109 -------------------------------------------------- ------------------------------------ 3.3103 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4694 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 526 ANIMAL NUMBER: C92399 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 242.0 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:30 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4695 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 527 ANIMAL NUMBER: C92400 SEX: FEMALE DATE OF DEATH: 07/07/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/07/98 9:01 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.4 GRAMS PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 8.5181 -------------------------------------------------- ------------------------------------ 3.0818 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT APPLICABLE GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4696 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 528 ANIMAL NUMBER: C92400 SEX: FEMALE DOSE GROUP: 8 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 276.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:01 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: HEMATO NEOPLASIA (HN) 4697 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 529 ANIMAL NUMBER: C92401 SEX: FEMALE DATE OF DEATH: 01/06/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/06/00 15:38 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 355.1 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK RED, TAN AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: CLEAR GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: RED HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-MODERATE, DIFFUSE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : ^UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4698 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 530 ANIMAL NUMBER: C92401 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/06/00 STUDY DAY OF DEATH: 641 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 355.1 GRAMS DATE AND TIME OF NECROPSY: 01/06/00 15:38 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY) 4699 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 531 ANIMAL NUMBER: C92402 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 200.5 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.3283 -------------------------------------------------- ------------------------------------ 3.6550 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4700 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 532 ANIMAL NUMBER: C92402 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 200.5 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:24 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4701 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 533 ANIMAL NUMBER: C92403 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 15:17 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 35; 10 X 10MM; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY HEART (HT) : -LIGHT FOCUS(I)/AREA(S); DIFFUSE, EPICARDIUM: MULTIPLE GRAY INDISTINCT AREAS LN, MEDIASTINAL (ML) : -DIFFUSELY RED; DARK RED (CAP 18) HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-SLIGHT, FOCAL HEART (HT) : -FIBROSIS,-MODERATE, MULTI-FOCAL -INFLAMMATION, CHRONIC,-MODERATE, MULTI FOCAL DEATH COMMENT (DC) : -UNDETERMINED,-PRESENT LN, MEDIASTINAL (ML) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4702 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 534 ANIMAL NUMBER: C92403 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 03/27/99 STUDY DAY OF DEATH: 356 STUDY WEEK OF DEATH: 51 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 03/27/99 15:17 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MEs /pANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY) 4703 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 535 ANIMAL NUMBER: C92404 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 453.4 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 10:30 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL-LEFT; WEEK 70; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 76; 50 X 45MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 79; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATELY SEVERE, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE, MULTI-FOCAL -VACUOLATION, HEPATOCELLULAR,-MINIMAL MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL SKIN (SK) : -FIBROSIS,-MODERATELY SEVERE, FOCAL -PIGMENT,-MODERATE, MULTI-FOCAL -M-CARCINOMA, SQUAMOUS CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 4704 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 536 ANIMAL NUMBER: C92404 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 453.4 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 10:30 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT LATERAL CERVICAL REGION: SINGLE MASS, 20 MM IN DIAMETER, RED, DARK RED, TAN, BROWN, SOFT, AND LOBULATED; SAME ON CUT SURFACE; OVERLYING SKIN HAS A DARK RED, DRY, CRUSTED AREA, 10 MM IN DIAMETER (CAP 21); REGIONAL LN (SEE MANDIBULAR) -THICKENED; ALL REGIONS, DIFFUSE: SOME AREAS ARE TAN, FIRM, AND LOBULATED (CAP 20) -MASS #2; LEFT LATERAL THORACIC AND LUMBAR REGIONS: SINGLE MASS, 85 X 60 X 30 MM, RED, DARK RED, TAN, BROWN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDED A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (SEE AXILLARY) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 5 MM, RED, GRAY, TAN, AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WHITE AND MUCOID BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4705 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 537 ANIMAL NUMBER: C92404 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 453.4 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 10:30 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: BROWN AND TAN (CAP 19) LN, AXILLARY (AX) : -DIFFUSELY RED; LEFT: DARK RED (CAP 18) -MOTTLED; RIGHT: DARK BROWN AND TAN (CAP 18) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; YELLOW AND MOIST LN, MANDIBULAR (MN) : >UNREMARKABLE LN, AXILLARY (AX) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -INFILTRATE, MACROPHAGE, VACUOLATED,SLIGHT, MULTI-FOCAL 4706 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 538 ANIMAL NUMBER: C92404 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 453.4 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 10:30 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY) 4707 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 539 ANIMAL NUMBER: C92405 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/20/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 02/20/00 7:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: NOT OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 12 X 9 X 6 MM, RED, DARK RED, TAN, GRAY, AND SOFT CECUM (CE) : -THICKENED WALL; DIFFUSE -MASS(ES); SINGLE MASS, 35 X 22 X 12 MM, RED, AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT WITH CECUM; REGIONAL LN (MESENTERIC) NORMAL HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATELY SEVERE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DEATH COMMENT (DC) : -MALIGNANT LYMPHOMA,-PRESENT 4708 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 540 ANIMAL NUMBER: C92405 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/20/00 STUDY DAY OF DEATH: 686 STUDY WEEK OF DEATH: 98 TERMINAL BODY WEIGHT: 364.2 GRAMS DATE AND TIME OF NECROPSY: 02/20/00 7:53 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN HEMATO NEOPLASIA (HN) : -M-LYMPHOMA,-NEOPLASM PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), LN, MESENTERIC (MS) 4709 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 541 ANIMAL NUMBER: C92406 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:53 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 331.3 GRAMS PROSECTOR: TODD ACKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0465 .0317 2.0870 1.3016 1.3809 9.2698 1.2827 .0475 .0543 .4773 .0105 .0107 1.3733 .0140 % .0096 % .6299 % .3929 % .4168 % 2.7980 % .3872 % .0143 % .0164 % .1441 % .0032 % .0032 % .4145 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0223 .0152 1.0000 .6237 .6617 4.4417 .6146 .0228 .0260 .2287 .0050 .0051 .6580 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -CYST(S); LEFT ON CORTICAL SURFACE, EXTENDS INTO MEDULLA: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN LUNG (LU) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL KIDNEY (KD) : -CYST,-MODERATE, FOCAL -HYPERPLASIA, TRANSITIONAL CELL,SLIGHT, DIFFUSE -INFLAMMATION, CHRONIC,-MODERATE, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4710 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 542 ANIMAL NUMBER: C92406 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 331.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:53 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) 4711 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 543 ANIMAL NUMBER: C92407 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 8:05 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 490.3 GRAMS PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0454 .0437 2.0210 1.4233 1.5123 13.2832 1.4892 .0476 .0541 .4835 .0241 .0211 1.1172 .0093 % .0089 % .4122 % .2903 % .3084 % 2.7092 % .3037 % .0097 % .0110 % .0986 % .0049 % .0043 % .2279 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0225 .0216 1.0000 .7043 .7483 6.5726 .7369 .0236 .0268 .2392 .0119 .0104 .5528 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL; COLLECTED GENERAL COMMENT (GC) : -ANIMAL OBESE -BONE MARROW SMEAR TAKEN KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL 4712 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 544 ANIMAL NUMBER: C92407 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 490.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:05 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL INFORMATION (XX) : >NOTE:>ANIMAL IDENTIFICATION TRANSPONDER DAMAGED AT NECROPSY THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) 4713 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 545 ANIMAL NUMBER: C92408 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 431.7 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 73; 100 X 85MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 92; 35 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EYES; PALE; EYES; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LARGE; ALL LOBES: MARGINS APPEAR ROUNDED -RAISED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF LEFT LATERAL LOBE: SINGLE AREA, 10 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 90 X 60 X 45 MM, RED, DARK RED, TAN, VASCULAR, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS: DIFFUSE; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,SLIGHT, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL -B-ADENOMA, HEPATOCELLULAR,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 4714 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 546 ANIMAL NUMBER: C92408 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 431.7 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 30 X 25 X 15 MM, RED, TAN, VASCULAR, LOBULATED, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 8 MM IN DIAMETER, DARK RED AND SOFT LN, AXILLARY (AX) : -LARGE; RIGHT: 12 X 7 X 3 MM 3 X 2 X 1 MM) (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN (LEFT IS BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : ^UNREMARKABLE 4715 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 547 ANIMAL NUMBER: C92408 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/27/00 STUDY DAY OF DEATH: 722 STUDY WEEK OF DEATH: 104 TERMINAL BODY WEIGHT: 431.7 GRAMS DATE AND TIME OF NECROPSY: 03/27/00 14:27 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) 4716 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 548 ANIMAL NUMBER: C92409 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 376.1 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 10:25 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 74; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 50 X 40 X 25 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; ON SECTION, SOME AREAS EXUDE A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 21) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER (CAP 25) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 24) -MASS #2; LEFT AXILLARY REGION: SINGLE MASS, 20 X 10 X 3 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (AXILLARY) NORMAL (CAP 21) HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -PIGMENT,-MODERATELY SEVERE, FOCAL -B-FIBROADENOMA,- 4 NEOPLASMS PRESENT 4717 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 549 ANIMAL NUMBER: C92409 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 376.1 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 10:25 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; RIGHT INGUINAL REGION: SINGLE MASS, 15 X 15 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 26); REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 12 X 8 X 7 MM (RIGHT IS 4 X 3 X 3 MM) -MOTTLED; LEFT: RED AND BROWN PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, RED, DARK RED, BROWN, AND SOFT CECUM (CE) : -THICKENED WALL; DIFFUSE UTERUS (UT) : -MASS(ES); ATTACHED TO ENDOMETRIUM OF RIGHT MIDDLE HORN: SINGLE POLYPOID MASS, 18 X 12 X 9 MM, RED, DARK RED, SOFT, AND GELATINOUS; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 27) VAGINA (VA) : -SEMIFLUID MATERIAL; RED SEMIFLUID BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT CECUM (CE) : >UNREMARKABLE UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4718 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 550 ANIMAL NUMBER: C92409 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/25/00 STUDY DAY OF DEATH: 691 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 376.1 GRAMS DATE AND TIME OF NECROPSY: 02/25/00 10:25 PROSECTOR: NANCY DIEDRICH RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) 4719 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 551 ANIMAL NUMBER: C92410 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 9:48 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 450.3 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 88; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 103; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: SINGLE RED FOCUS, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 15 X 10 X 6 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 22); REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT CERVICAL REGION: SINGLE MASS, 40 X 20 X 20 MM, RED, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE; SOME SECTIONS EXUDE A DARK RED SEMIFLUID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 23) HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-SLIGHT, MULTI-FOCAL -TELANGIECTASIS,-MINIMAL, FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,- 2 NEOPLASMS PRESENT 4720 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 552 ANIMAL NUMBER: C92410 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 450.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 9:48 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 4 MM, RED, TAN AND SOFT RED, DARK BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), GENERAL COMMENT (GC), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB), LN, MANDIBULAR (MN) 4721 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 553 ANIMAL NUMBER: C92411 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 11:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 392.4 GRAMS PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0602 .0424 2.2159 1.5354 1.7134 12.1056 1.6581 .0873 .0786 .5287 .0150 .0163 1.3063 .0153 % .0108 % .5647 % .3913 % .4366 % 3.0850 % .4226 % .0222 % .0200 % .1347 % .0038 % .0042 % .3329 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0272 .0191 1.0000 .6929 .7732 5.4631 .7483 .0394 .0355 .2386 .0068 .0074 .5895 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 44; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 15 X 10 X 5 MM, RED, TAN AND FIRM; SAME ON CUT SURFACE (CAP 18); REGIONAL LN (AXILLARY) NORMAL (CAP 19) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE CREAMY SEMIFLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4722 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 554 ANIMAL NUMBER: C92411 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 392.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:10 PROSECTOR: MONTI THOMAS MAGUIRE RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -MOTTLED; BILATERAL: RED AND DARK RED; COLLECTED GENERAL COMMENT (GC) : -MALOCCLUSION; BILATERAL UPPER INCISORS -BONE MARROW SMEAR TAKEN LN, MANDIBULAR (MN) : ^UNREMARKABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) 4723 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 555 ANIMAL NUMBER: C92412 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 11:42 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 465.8 GRAMS PROSECTOR: JARED FISHEL RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PITUITARY (PI) : -RED FOCUS(I)/AREA(S); ANTERIOR ASPECT: SINGLE DARK RED AND RAISED AREA, 3 MM IN DIAMETER OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, FOCAL -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4724 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 556 ANIMAL NUMBER: C92412 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 465.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:42 PROSECTOR: JARED FISHEL RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), URINARY BLADDER (UB) 4725 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 557 ANIMAL NUMBER: C92413 SEX: FEMALE DATE OF DEATH: 03/15/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/15/00 15:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 710 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 456.5 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 96; 55 X 50MM; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 8 X 6 X 5 MM (RIGHT IS 6 X 3 X 3 MM) HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MODERATE, DIFFUSE -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4726 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 558 ANIMAL NUMBER: C92413 SEX: FEMALE DATE OF DEATH: 03/15/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/15/00 15:09 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 710 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 456.5 GRAMS PROSECTOR: TODD ACKER RECORDER: TODD ACKER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 12 X 7 X 6 MM, DARK RED, RED, TAN AND SOFT FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HINDFOOT: SINGLE RED, TAN AND DRY AREA, 3 MM IN DIAMETER; COLLECTED ADIPOSE TISSUE (AT) : -THICKENED; RIGHT AXILLARY REGION: SINGLE TAN AND FIRM AREA, 60 X 50 X 20 MM; COLLECTED; POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS -TEETH MISSING/BROKEN/WORN; BILATERAL, UPPER INCISORS: BROKEN AT BASE ADRENAL, MEDULLA (MA) : -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : -FIBROSIS,-MODERATE, FOCAL ADIPOSE TISSUE (AT) : -B-LIPOMA,-NEOPLASM PRESENT 4727 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 559 ANIMAL NUMBER: C92413 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/15/00 STUDY DAY OF DEATH: 710 STUDY WEEK OF DEATH: 102 TERMINAL BODY WEIGHT: 456.5 GRAMS DATE AND TIME OF NECROPSY: 03/15/00 15:09 PROSECTOR: TODD ACKER RECORDER: TODD ACKER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA) 4728 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 560 ANIMAL NUMBER: C92414 SEX: FEMALE DATE OF DEATH: 06/14/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/14/99 14:08 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 513.7 GRAMS PROSECTOR: TODD ACKER RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 44; 100 X 80MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 120 X 80 X 50 MM (173.8 GRAMS), RED, DARK RED, TAN, VASCULAR AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, FOCAL DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT 4729 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 561 ANIMAL NUMBER: C92414 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 513.7 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 14:08 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI) 4730 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 562 ANIMAL NUMBER: C92415 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 11:24 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -UNCOLLAPSED; ALL LOBES MAMMARY, FEMALE (MF) : -CYST(S); LEFT INGUINAL REGION: SINGLE TAN, CREAMY SEMIFLUID FILLED CYST, 25 X 25 X 5 MM; TISSUE SURROUNDING IS THICKENED, TAN AND FIRM (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 9 X 7 MM, RED, DARK RED, GREY, LOBULATED AND SOFT OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-SLIGHT, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATELY SEVERE, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MINIMAL, FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, CHRONIC-ACTIVE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT 4731 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 563 ANIMAL NUMBER: C92415 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/15/99 11:24 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -ANIMAL OBESE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: THYROID (TY) 4732 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 564 ANIMAL NUMBER: C92416 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 416.9 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); TAIL-PROXIMAL; WEEK 75; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED >WART LIKE LESION(S); HEAD-MAXILLARYRIGHT; WEEK 79; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADCRANIAL; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; TAIL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : -CRUSTED AREA(S); RIGHT EYELID: SINGLE DRY, TAN CRUSTED AREA, 6 X 4 MM (CAP 22) TAIL (TI) : -ULCERATION(S)/ABRASION(S); 25 MM FROM BASE OF TAIL: SINGLE TAN, RED, MOIST, RAISED ULCERATED AREA, 22 X 19 X 14 MM; COLLECTED; POSSIBLY CONSISTENT WITH MASS (TAIL) AS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN -STAINS - SKIN/HAIRCOAT; ENTIRE HEAD: DRY RED MATERIAL HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL THYROID (TY) : -HYPERPLASIA, C-CELL,-SLIGHT, FOCAL SKIN (SK) : -B-PAPILLOMA, SQUAMOUS CELL,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT TAIL (TI) : -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, FOCAL -M-FIBROSARCOMA,-NEOPLASM PRESENT 4733 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 565 ANIMAL NUMBER: C92416 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 416.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:30 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) 4734 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 566 ANIMAL NUMBER: C92417 SEX: FEMALE DATE OF DEATH: 01/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/18/00 10:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 237.7 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 7 MM IN DIAMETER (LEFT IS 4 MM IN DIAMETER) -MOTTLED; RIGHT: BROWN AND RED PITUITARY (PI) : -MASS(ES); 12 X 10 X 6 MM, DARK RED, LOBULATED AND FIRM HISTOPATHOLOGY LUNG (LU) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, MULTI FOCAL -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -VACUOLATION,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4735 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 567 ANIMAL NUMBER: C92417 SEX: FEMALE DATE OF DEATH: 01/18/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/18/00 10:16 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 237.7 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DRY BROWN MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY RED MATERIAL -ANIMAL THIN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4736 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 568 ANIMAL NUMBER: C92417 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/18/00 STUDY DAY OF DEATH: 653 STUDY WEEK OF DEATH: 94 TERMINAL BODY WEIGHT: 237.7 GRAMS DATE AND TIME OF NECROPSY: 01/18/00 10:16 PROSECTOR: JAMES T. CALLEN RECORDER: JAMES T. CALLEN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (MF), (ST), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY) 4737 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 569 ANIMAL NUMBER: C92418 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 11:10 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 427.0 GRAMS PROSECTOR: TODD ACKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0407 .0289 2.0241 1.2464 1.2915 11.4976 1.4056 .0639 .0487 .5717 .0150 .0113 1.0721 .0095 % .0068 % .4740 % .2919 % .3025 % 2.6926 % .3292 % .0150 % .0114 % .1339 % .0035 % .0026 % .2511 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0201 .0143 1.0000 .6158 .6381 5.6804 .6944 .0316 .0241 .2824 .0074 .0056 .5297 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4738 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 570 ANIMAL NUMBER: C92418 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 427.0 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 11:10 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS),SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) 4739 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 571 ANIMAL NUMBER: C92419 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:32 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 96; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 101; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -CYST(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE CLEAR FLUID FILLED CYST, 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 16 X 8 X 8 MM, TAN, DARK BROWN, FIRM AND LOBULATED (CAP 22); SAME ON CUT SURFACE; REGIONAL LN (SEE AXILLARY) -THICKENED; ALL REGIONS DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION -MASS #2; RIGHT AXILLARY REGION: SINGLE MASS, 35 X 25 X 21 MM, RED, TAN, FIRM AND LOBULATED; ON SECTION EXUDES A TAN CREAMY SEMISOLID MATERIAL; COLLECTED; REGIONAL LN (SEE AXILLARY) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY KIDNEY (KD) : -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL -PYELONEPHRITIS,-MINIMAL, MULTI-FOCAL LIVER (LI) : -DEGENERATION, CYSTIC,-MODERATE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT 4740 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 572 ANIMAL NUMBER: C92419 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:32 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 6 MM IN DIAMETER (LEFT IS 3 MM IN DIAMETER) PITUITARY (PI) : -MASS(ES); 10 X 8 X 4 MM, DARK RED, GRAY, SOFT AND LOBULATED UTERUS (UT) : -CYST(S); WITHIN WALL OF RIGHT HORN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 7 X 6 X 6 MM LN, AXILLARY (AX) : -MOTTLED; BILATERAL: DARK RED AND BROWN (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL LOWER INCISORS -BONE MARROW SMEAR TAKEN -TEETH MISSING/BROKEN/WORN; RIGHT UPPER INCISOR: BROKEN OFF AT BASE ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATE, DIFFUSE -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATELY SEVERE, MULTI-FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -INFILTRATE, MACROPHAGE, VACUOLATED,MINIMAL, MULTI-FOCAL 4741 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 573 ANIMAL NUMBER: C92419 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 402.7 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:32 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) 4742 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 574 ANIMAL NUMBER: C92420 SEX: FEMALE DATE OF DEATH: 08/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/23/99 13:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 569.1 GRAMS PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 59; 55 X 60MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; AXILLARY REGION-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT SHOULDER REGION: SINGLE MASS, 68 X 51 X 43 MM, RED, DARK RED, TAN, FIRM AND LOBULATED; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DRY RED CRUSTED AREA, 9 X 4 MM; COLLECTED INTACT; POSSIBLY CONSISTENT WITH SCAB (RIGHT AXILLARY) AS NOTED IN CLINICAL OBSERVATIONS; REGIONAL LN (AXILLARY) NORMAL (CAP 18) -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -LARGE; 6 X 6 X 4 MM; APPEARS SWOLLEN UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 MM IN DIAMETER HISTOPATHOLOGY LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-SLIGHT, MULTI FOCAL -PIGMENT,-SLIGHT, FOCAL -M-CARCINOMA,-NEOPLASM PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MODERATELY SEVERE, FOCAL -B-POLYP, ENDOMETRIAL, STROMAL,NEOPLASM PRESENT 4743 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 575 ANIMAL NUMBER: C92420 SEX: FEMALE DATE OF DEATH: 08/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/23/99 13:40 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 505 STUDY WEEK OF DEATH: 73 TERMINAL BODY WEIGHT: 569.1 GRAMS PROSECTOR: TONY HANFELD RECORDER: JARED FISHEL PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -MASS(ES); RIGHT HORN, WITHIN LUMEN, ATTACHED TO WALL: SINGLE MASS, 19 X 6 X 6 MM, TAN AND SOFT; SAME ON CUT SURFACE; COLLECTED INTACT; REGIONAL LN (LUMBAR) NORMAL (CAP 19) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN DEATH COMMENT (DC) : -MAMMARY CARCINOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX), LN, LUMBAR/ILIAC (LM) 4744 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 576 ANIMAL NUMBER: C92421 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 12:51 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN AREAS, UP TO 4 X 3 MM SKIN (SK) : -CRUSTED AREA(S); MID PERIANAL REGION: SINGLE DRY, TAN CRUSTED AREA, 7 MM IN DIAMETER (CAP 22) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 8 X 5 MM, RED, DARK RED, AND FIRM HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MODERATE, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL SKIN (SK) : -INFLAMMATION, CHRONIC-ACTIVE,MODERATE, MULTI-FOCAL -B-KERATOACANTHOMA,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4745 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 577 ANIMAL NUMBER: C92421 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 446.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:51 PROSECTOR: JAMES T. CALLEN RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS 11 X 7 X 4 MM (CAP 23) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN LN, MANDIBULAR (MN) : -HYPERPLASIA, LYMPHOCYTIC,-SLIGHT, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) 4746 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 578 ANIMAL NUMBER: C92422 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:56 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 405.4 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0352 .0282 2.1483 1.1812 1.1924 8.9117 1.4161 .0726 .0724 .5826 .0173 .0231 .5629 .0087 % .0070 % .5299 % .2914 % .2941 % 2.1982 % .3493 % .0179 % .0179 % .1437 % .0043 % .0057 % .1389 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0164 .0131 1.0000 .5498 .5550 4.1483 .6592 .0338 .0337 .2712 .0081 .0108 .2620 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4747 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 579 ANIMAL NUMBER: C92422 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 405.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:56 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS),SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) 4748 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 580 ANIMAL NUMBER: C92423 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:33 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 566.4 GRAMS PROSECTOR: JODI THIEREN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY PARATHYROID (PT) : -LARGE; RIGHT: 4 X 3 X 2 MM (LEFT IS 1 MM IN DIAMETER) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, RED, RED, GRAY AND FIRM DARK OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -B-ADENOMA, C-CELL,-NEOPLASM PRESENT PARATHYROID (PT) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : >UNREMARKABLE 4749 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 581 ANIMAL NUMBER: C92423 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 566.4 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:33 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), URINARY BLADDER (UB) 4750 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 582 ANIMAL NUMBER: C92424 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 8:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 399.4 GRAMS PROSECTOR: TODD ACKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0428 .0470 1.9919 1.3161 1.3429 10.7548 1.5499 .0701 .0572 .5224 .0323 .0121 1.3871 .0107 % .0118 % .4987 % .3295 % .3362 % 2.6927 % .3881 % .0176 % .0143 % .1308 % .0081 % .0030 % .3473 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0215 .0236 1.0000 .6607 .6742 5.3993 .7781 .0352 .0287 .2623 .0162 .0061 .6964 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED UTERUS (UT) : -CYST(S); WITHIN WALL OF RIGHT HORN: SINGLE CLEAR FLUID FILLED CYST, 15 X 5 X 5 MM GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN THYROID (TY) : -CYST, FOLLICULAR,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4751 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 583 ANIMAL NUMBER: C92424 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 399.4 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:03 PROSECTOR: TODD ACKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA) 4752 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 584 ANIMAL NUMBER: C92425 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 13:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 555.1 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-RIGHT; WEEK 84; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 92; 30 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 50 X 30 X 25 MM, RED, TAN, FIRM, AND LOBULATED; SOME SECTIONS EXUDE A TAN CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT PERIANAL REGION: SINGLE MASS, 45 X 30 X 15 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED; OVERLYING SKIN HAS A SINGLE RED AND TAN AREA, EXTENDING INTO LEFT INGUINAL REGION, 50 X 30 MM; COLLECTED WITH MASS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT, MULTI-FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT 4753 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 585 ANIMAL NUMBER: C92425 SEX: FEMALE DATE OF DEATH: 02/28/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 02/28/00 13:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 555.1 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY SPLEEN (SP) : -LARGE; 52 X 12 X 8 MM PITUITARY (PI) : -MASS(ES); 6 X 5 X 3 MM, RED, TAN, GREY, AND SOFT OVARY (OV) : -CYST(S); RIGHT: SINGLE RED FLUID FILLED CYST, 5 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -PIGMENT,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -MAMMARY CARCINOMA,-PRESENT 4754 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 586 ANIMAL NUMBER: C92425 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 555.1 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:50 PROSECTOR: TERRI L. WILLIAMS RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA) 4755 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 587 ANIMAL NUMBER: C92426 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 12:42 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 377.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYE-LEFT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); DORSAL; WEEK 83; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 101; 70 X 65MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS SKIN (SK) : -MASS(ES); DORSAL SURFACE OF RIGHT LUMBAR REGION: SINGLE MASS, 5 X 15 X 18 MM, FIRM, RED, TAN AND BROWN; SAME ON CUT SURFACE; OVERLYING SKIN HAS A DRY, BROWN CRUSTED AREA, 8 MM IN DIAMETER (CAP 23); REGIONAL LN (SEE LUMBAR) MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE FIRM, LOBULATED, RED AND TAN MASS, 31 X 45 X 60 MM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-SLIGHT, FOCAL LIVER (LI) : >UNREMARKABLE MULTI THYROID (TY) : -B-ADENOMA, FOLLICULAR CELL,-NEOPLASM PRESENT SKIN (SK) : -CYST, EPIDERMAL INCLUSION,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT 4756 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 588 ANIMAL NUMBER: C92426 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 12:42 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 377.6 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 6 X 8 FIRM, RED, DARK RED AND TAN MM, FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE DRY, RED, BROWN ABRADED AREA, 8 X 12 MM COLLECTED >NOTE:>LEFT HINDFOOT SORE/SCAB NOT SEEN AS NOTED IN CLINICAL OBSERVATIONS LN, LUMBAR/ILIAC (LM) : -LARGE; RIGHT, LUMBAR: 4 X 6 X 9 MM (LEFT IS 2 X 2 X 4 MM) (CAP 24) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DRY, RED MATERIAL -BONE MARROW SMEAR TAKEN BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -EROSION,-MODERATELY SEVERE, FOCAL -EXOSTOSES,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, ACUTE,-MODERATELY SEVERE, MULTI-FOCAL LN, LUMBAR/ILIAC (LM) : >UNREMARKABLE 4757 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 589 ANIMAL NUMBER: C92426 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 377.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 12:42 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), URINARY BLADDER (UB) 4758 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 590 ANIMAL NUMBER: C92427 SEX: FEMALE DATE OF DEATH: 06/14/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/14/99 13:47 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 542.3 GRAMS PROSECTOR: TODD ACKER RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 55; 110 X 100MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; LATERALABDOMINAL-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 120 X 90 X 80 MM (291.6 GRAMS), RED, DARK RED, TAN, VASCULAR AND SOFT; SAME ON CUT SURFACE; PORTION COLLECTED; OVERLYING SKIN HAS A SINGLE DARK RED, TAN AND DRY CRUSTED AREA, 35 X 20 MM; COLLECTED INTACT WITH MASS; REGIONAL LN (AXILLARY) NOT IDENTIFIED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LIVER (LI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -NECROSIS, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -MAMMARY FIBROADENOMA,-PRESENT 4759 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 591 ANIMAL NUMBER: C92427 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/14/99 STUDY DAY OF DEATH: 435 STUDY WEEK OF DEATH: 63 TERMINAL BODY WEIGHT: 542.3 GRAMS DATE AND TIME OF NECROPSY: 06/14/99 13:47 PROSECTOR: TODD ACKER RECORDER: JODI THIEREN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY) 4760 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 592 ANIMAL NUMBER: C92428 SEX: FEMALE DATE OF DEATH: 06/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/07/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 384.4 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-LEFT; WEEK 51; 85 X 75MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRALABDOMINAL-LEFT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION, EXTENDING TO MID VENTRAL CERVICAL REGION: SINGLE MASS, 100 X 80 X 50 MM, RED, DARK RED, TAN, FIRM, AND LOBULATED; SOME AREAS EXUDE A DARK BROWN CREAMY SEMIFLUID MATERIAL; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) SPLEEN (SP) : -DIFFUSELY LIGHT; TAN ADRENAL, CORTEX (AC) : -LARGE; BILATERAL: LEFT IS 9 MM IN DIAMETER, RIGHT IS 7 MM IN DIAMETER HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, FOCAL LIVER (LI) : -HEMORRHAGE,-MINIMAL, FOCAL -NECROSIS, HEPATOCELLULAR,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MODERATE, DIFFUSE ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, FOCAL MULTI 4761 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 593 ANIMAL NUMBER: C92428 SEX: FEMALE DATE OF DEATH: 06/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 06/07/99 14:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 384.4 GRAMS PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -LARGE; 7 X 5 X 3 MM; APPEARS SWOLLEN SALIV GL, MANDIB (SG) : >NOTE:>DUE TO MASS, LEFT SALIVARY GLAND IS MISSING STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE DARK BROWN AREAS, UP TO 3 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; DARK RED, DRY MATERIAL -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, FOCAL -HEMATOPOIESIS, EXTRAMEDULLARY,-SLIGHT, MULTI-FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT SALIV GL, MANDIB (SG) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL STOMACH, GL (ST) : >UNREMARKABLE DEATH COMMENT (DC) : -MAMMARY CARCINOMA,-PRESENT 4762 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 594 ANIMAL NUMBER: C92428 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/07/99 STUDY DAY OF DEATH: 428 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: 384.4 GRAMS DATE AND TIME OF NECROPSY: 06/07/99 14:15 PROSECTOR: MICHELE MARGGI RECORDER: MICHELE MARGGI POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) 4763 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 595 ANIMAL NUMBER: C92429 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/10/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/10/99 14:30 PROSECTOR: DAVID SCHUETTE RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY LUNG (LU) : -UNCOLLAPSED; ALL LOBES -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE TAN, SLIGHTLY RAISED AREAS, UP TO 4 MM IN DIAMETER (COLLECTED WITH ROUTINE SECTIONS) SPLEEN (SP) : -LARGE; 60 X 12 X 6 MM CECUM (CE) : -MASS(ES); WITHIN LUMEN, ATTACHED TO MUCOSA: SINGLE MASS, 40 X 25 X 20 MM, DARK RED, TAN AND SOFT; SAME ON CUT SURFACE (COLLECTED INTACT); REGIONAL LN (MESENTERIC) NORMAL HISTOPATHOLOGY LUNG (LU) : >UNREMARKABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LIVER (LI) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-SLIGHT, MULTI-FOCAL CECUM (CE) : -X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE),-NEOPLASM PRESENT DEATH COMMENT (DC) : -HISTIOCYTIC SARCOMA,-PRESENT 4764 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 596 ANIMAL NUMBER: C92429 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 06/10/99 STUDY DAY OF DEATH: 431 STUDY WEEK OF DEATH: 62 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 06/10/99 14:30 PROSECTOR: DAVID SCHUETTE RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; RIGHT: DRY DARK BROWN MATERIAL -DISCHARGE-NASAL; DRY BROWN MATERIAL HEMATO NEOPLASIA (HN) : -M-SARCOMA, HISTIOCYTIC,-NEOPLASM PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) 4765 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 597 ANIMAL NUMBER: C92430 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 7:59 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 506.9 GRAMS PROSECTOR: WENDY CHERRY RECORDER: DAVID SCHUETTE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDLEFT; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 102; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DARK FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: SINGLE DARK BROWN AREA, 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -MASS(ES); MID ABDOMINAL REGION: SINGLE MASS, 60 X 45 X 15 MM, GRAY, DARK RED AND SOFT; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 9 X 6 MM, DARK RED, TAN AND SOFT HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LIVER (LI) : >UNREMARKABLE THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4766 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 598 ANIMAL NUMBER: C92430 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 506.9 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 7:59 PROSECTOR: WENDY CHERRY RECORDER: DAVID SCHUETTE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); LEFT HINDFOOT: SINGLE DARK RED AND TAN ABRADED AREA, 8 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -CYST,-PRESENT -FIBROSIS,-MODERATE, DIFFUSE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), URINARY BLADDER (UB) 4767 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 599 ANIMAL NUMBER: C92431 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:30 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.8 GRAMS PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 95; 50 X 50MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-RIGHT; WEEK 96; 40 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 40 X 27 X 15 MM, DARK RED, TAN AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 55 X 55 X 20 MM, TAN, GRAY AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, FOCAL PANCREAS (PA) : -B-ADENOMA, ISLET CELL,-NEOPLASM PRESENT THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL 4768 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 600 ANIMAL NUMBER: C92431 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.8 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:30 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 6 X 5 X 4 MM, DARK RED, GRAY AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), URINARY BLADDER (UB) 4769 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 601 ANIMAL NUMBER: C92432 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 312.1 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 12:47 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-THORACIC-LEFT; WEEK 65; 50 X 45MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >RESPIRATION; LABORED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION EXTENDING TO LATERAL THORACIC REGION: SINGLE MASS, 55 X 50 X 24 MM, RED, TAN, FIRM AND LOBULATED; SOME SECTIONS EXUDE A CREAMY WHITE SEMIFLUID; COLLECTED INTACT; REGIONAL LN (AXILLARY) NORMAL (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 9 X 7 X 6 MM (LEFT IS 6 X 4 X 3 MM) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 13 X 8 X 6 MM, RED, DARK RED, TAN, SOFT AND LOBULATED UTERUS (UT) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 5 MM IN DIAMETER HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-MINIMAL, DIFFUSE 4770 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 602 ANIMAL NUMBER: C92432 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 09/24/99 STUDY DAY OF DEATH: 537 STUDY WEEK OF DEATH: 77 TERMINAL BODY WEIGHT: 312.1 GRAMS DATE AND TIME OF NECROPSY: 09/24/99 12:47 PROSECTOR: JARED FISHEL RECORDER: JARED FISHEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: WHITE MUCOID MATERIAL LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: RED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL, UPPER INCISORS VAGINA (VA) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) 4771 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 603 ANIMAL NUMBER: C92433 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:04 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0407 .0424 1.9586 1.2071 1.1803 10.6892 1.5529 .0273 .0651 .5780 .0184 .0144 .9246 .0112 % .0117 % .5406 % .3332 % .3258 % 2.9504 % .4286 % .0075 % .0180 % .1595 % .0051 % .0040 % .2552 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0208 .0216 1.0000 .6163 .6026 5.4576 .7929 .0139 .0332 .2951 .0094 .0074 .4721 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 10 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL 4772 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 604 ANIMAL NUMBER: C92433 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 362.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:04 PROSECTOR: TONY HANFELD RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, MANDIBULAR (MN) : -DIFFUSELY RED; BILATERAL: COLLECTED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN DARK RED; DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), THYROID (TY) 4773 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 605 ANIMAL NUMBER: C92434 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 11:26 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); SHOULDER-RIGHT; WEEK 55; 80 X 65MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 70; 40 X 50MM ANTERIOR; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 74; 100 X 75MM POSTERIOR; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 80 X 55 X 25 MM, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 50 X 40 X 35 MM, RED, DARK RED, GRAY, TAN, LOBULATED, AND FIRM; SOME SECTIONS EXUDE A TAN AND CREAMY SEMIFLUID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY LUNG (LU) : -HYPERPLASIA, TYPE II PNEUMOCYTE,MINIMAL, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,- 3 NEOPLASMS PRESENT 4774 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 606 ANIMAL NUMBER: C92434 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 11:26 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; RIGHT INGUINAL REGION, CAUDAL TO MASS #2: SINGLE MASS, 80 X 60 X 45 MM, RED, DARK RED, TAN, LOBULATED AND FIRM; SOME SECTIONS EXUDE A DARK RED SEMISOLID; PORTION COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 MM IN DIAMETER (LEFT IS 5 MM IN DIAMETER) PITUITARY (PI) : -MASS(ES); 10 X 7 X 6 MM, DARK RED, TAN, GRAY AND SOFT UTERUS (UT) : -CYST(S); RIGHT HORN, WITHIN WALL: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 5 X 3 X 3 MM BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-SLIGHT, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,SEVERE, FOCAL -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -THROMBUS,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -M-CARCINOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,SLIGHT, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY CARCINOMA,-PRESENT 4775 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 607 ANIMAL NUMBER: C92434 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 12/29/99 STUDY DAY OF DEATH: 633 STUDY WEEK OF DEATH: 91 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 12/29/99 11:26 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY) 4776 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 608 ANIMAL NUMBER: C92435 SEX: FEMALE DATE OF DEATH: 04/28/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/28/98 8:47 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 241.8 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 10.0261 -------------------------------------------------- ------------------------------------ 4.1464 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4777 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 609 ANIMAL NUMBER: C92435 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 241.8 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:47 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4778 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 610 ANIMAL NUMBER: C92436 SEX: FEMALE DATE OF DEATH: 01/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/31/00 14:02 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 421.7 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 66; 50 X 60MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 74; 40 X 50MM; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY SKIN (SK) : >NOTE:>DUE TO LOCATION OF MASS, ROUTINE SECTION OF SKIN/MAMMARY GLAND COLLECTED FROM RIGHT INGUINAL REGION MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 60 X 55 X 28 MM, RED, DARK RED, TAN, BROWN, LOBULATED, CYSTIC, AND SOFT; ON SECTION, EXUDES A YELLOW CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 19) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN, CREAMY SEMIFLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL SKIN (SK) : >NOT REQUIRED TO BE EXAMINED FOR ANIMAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SEVERE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT 4779 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 611 ANIMAL NUMBER: C92436 SEX: FEMALE DATE OF DEATH: 01/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/31/00 14:02 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 421.7 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 72 X 53 X 48 MM, RED, DARK RED, TAN, BROWN, CYSTIC, LOBULATED, VASCULAR, AND SOFT; ON SECTION, EXUDES A TAN, CREAMY SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED >NOTE:>DUE TO LOCATION OF MASS, ROUTINE SECTION OF SKIN/MAMMARY GLAND COLLECTED FROM RIGHT INGUINAL REGION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 9 X 7 X 5 MM, RED, DARK RED, TAN, LOBULATED AND SOFT OVARY (OV) : -CYST(S); BILATERAL: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 2 MM IN DIAMETER, RIGHT IS 5 MM IN DIAMETER CERVIX (CV) : -LARGE; UP TO 14 MM IN DIAMETER BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT CERVIX (CV) : -DILATATION,-SEVERE, FOCAL -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -M-SARCOMA, ENDOMETRIAL STROMAL,NEOPLASM PRESENT 4780 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 612 ANIMAL NUMBER: C92436 SEX: FEMALE DATE OF DEATH: 01/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/31/00 14:02 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 421.7 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: TAN, MUCOID SEMIFLUID -THICKENED WALL; DIFFUSE LN, MANDIBULAR (MN) : -LARGE; BILATERAL: EACH IS UP TO 7 X 5 X 3 MM (CAP 18) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY, CRUSTED MATERIAL -ANIMAL THIN VAGINA (VA) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL -N-SARCOMA, ENDOMETRIAL STROMAL,NEOPLASM PRESENT DEATH COMMENT (DC) : -ENDOMETRIAL STROMAL SARCOMA,-PRESENT LN, MANDIBULAR (MN) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL 4781 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 613 ANIMAL NUMBER: C92436 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 421.7 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 14:02 PROSECTOR: STEVE VAN ADESTINE RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) 4782 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 614 ANIMAL NUMBER: C92437 SEX: FEMALE DATE OF DEATH: 03/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/19/99 7:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 262.3 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL-LEFT; WEEK 34; 30 X 20MM; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT INGUINAL REGION: SINGLE MASS, 30 X 22 X 14 MM, RED, DARK RED, TAN, GRAY, LOBULATED AND FIRM; SAME ON CUT SURFACE (CAP 19); REGIONAL LN (INGUINAL) LEFT IS NORMAL, RIGHT NOT IDENTIFIED (CAP 20) -CYST(S); BILATERAL AXILLARY REGIONS: MULTIPLE TAN AND CREAMY SEMIFLUID FILLED CYSTS, UP TO 8 X 7 X 7 MM (CAP 21) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 10 X 8 MM, RED, DARK RED, GRAY AND SOFT STOMACH, NONGL (SU) : -THICKENED WALL; DIFFUSE VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: MODERATE AMOUNT OF A LIGHT GREEN AND MUCOID SEMIFLUID MATERIAL HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, FOCAL -MINERALIZATION,-SLIGHT, FOCAL -M-CARCINOMA,-NEOPLASM PRESENT MULTI BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EDEMA,-MINIMAL, FOCAL VAGINA (VA) : >UNREMARKABLE 4783 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 615 ANIMAL NUMBER: C92437 SEX: FEMALE DATE OF DEATH: 03/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 03/19/99 7:52 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 348 STUDY WEEK OF DEATH: 50 TERMINAL BODY WEIGHT: 262.3 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JODI THIEREN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY LN, AXILLARY (AX) : -LARGE; LEFT: 15 X 12 X 10 MM (FOR COMPARISON, RIGHT IS 6 X 1 X 1 MM) (CAP 18) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED AND DRY MATERIAL -ANIMAL THIN DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : >UNREMARKABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARYBLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN),AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, INGUINAL (IN) 4784 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 616 ANIMAL NUMBER: C92438 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 10:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 348.6 GRAMS PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0325 .0300 1.8684 1.1359 1.1676 9.3979 1.3652 .0463 .0548 .6518 .0119 .0118 1.0419 .0093 % .0086 % .5360 % .3258 % .3349 % 2.6959 % .3916 % .0133 % .0157 % .1870 % .0034 % .0034 % .2989 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0174 .0161 1.0000 .6080 .6249 5.0299 .7307 .0248 .0293 .3489 .0064 .0063 .5576 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >MASS(ES); VENTRAL-ABDOMINAL; WEEK 44; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT 4785 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 617 ANIMAL NUMBER: C92438 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 348.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 10:15 PROSECTOR: GREGORY CODDINGTON RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DONIA MEYER LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -CYST(S); BILATERAL INGUINAL REGIONS: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 12 X 8 X 7 MM (CAP 18); POSSIBLY CONSISTENT WITH MASS NOTED IN CLINICAL OBSERVATIONS GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU) 4786 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 618 ANIMAL NUMBER: C92439 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 8:04 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.6 GRAMS PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 5 MM IN DIAMETER, SUNKEN WITH A STELLATE PATTERN; COLLECTED ON ROUTINE SECTIONS OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 12 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -CYST,-SLIGHT, FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-SLIGHT, FOCAL -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4787 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 619 ANIMAL NUMBER: C92439 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 438.6 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:04 PROSECTOR: TROY ELLENBECKER RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) 4788 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 620 ANIMAL NUMBER: C92440 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:41 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); HEAD-MAXILLARY-RIGHT; WEEK 66; 10 X 10MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; HEAD MAXILLARY-RIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; LIMITED USE; LIMB-HINDLEFT; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LUNG (LU) : -UNCOLLAPSED; ALL LOBES SKIN (SK) : -MASS(ES); ATTACHED TO RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 11 X 10 X 5 MM, RED, DARK RED, GREY AND FIRM; SAME ON CUT SURFACE (CAP 18); REGIONAL LN (MANDIBULAR) NORMAL (CAP 19); OVERLYING SKIN HAS A SINGLE DRY, DARK RED CRUSTED AREA, 4 MM IN DIAMETER; COLLECTED WITH MASS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 5 MM IN DIAMETER (RIGHT IS 3 MM IN DIAMETER) HISTOPATHOLOGY LUNG (LU) : >UNREMARKABLE KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL -PYELONEPHRITIS,-SLIGHT, FOCAL SKIN (SK) : -B-BASAL CELL TUMOR,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MINIMAL, MULTI-FOCAL -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -THROMBUS,-MODERATELY SEVERE, FOCAL 4789 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 621 ANIMAL NUMBER: C92440 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:41 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 11 X 10 X 9 MM, DARK RED, LOBULATED AND SOFT FOOT/FOOTPAD (FP) : -LARGE; LEFT HINDFOOT: 44 X 18 X 18 XX (RIGHT IS 44 X 10 X 9 MM); LEFT APPEARS SWOLLEN; BOTH COLLECTED GENERAL COMMENT (GC) : -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : -EXOSTOSES,-SLIGHT, MULTI-FOCAL -FIBROSIS,-SEVERE, MULTI-FOCAL -INFLAMMATION, CHRONIC ACTIVE,MODERATE, MULTI-FOCAL 4790 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 622 ANIMAL NUMBER: C92440 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (D) DATE OF DEATH: 10/17/99 STUDY DAY OF DEATH: 560 STUDY WEEK OF DEATH: 80 TERMINAL BODY WEIGHT: NOT ENTERED DATE AND TIME OF NECROPSY: 10/17/99 9:41 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF), ADRENAL, MEDULLA (MA), LN, MANDIBULAR (MN) 4791 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 623 ANIMAL NUMBER: C92441 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 9:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 450.3 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0457 .0428 2.0955 1.6868 1.6612 12.7468 1.6925 .0693 .0623 .8104 .0121 .0106 1.1020 .0101 % .0095 % .4654 % .3746 % .3689 % 2.8307 % .3759 % .0154 % .0138 % .1800 % .0027 % .0024 % .2447 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0218 .0204 1.0000 .8050 .7927 6.0829 .8077 .0331 .0297 .3867 .0058 .0051 .5259 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >SKIN & PELAGE; SORE/SCAB; DORSAL; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-LEFT; WEEK 48; 20 X 15MM; VERIFIED AT NECROPSY: OBSERVED SKIN (SK) : -CRUSTED AREA(S); RIGHT FLANK: SINGLE TAN, DRY AREA, 8 MM IN DIAMETER (CAP 19) MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 13 MM IN DIAMETER, TAN, BROWN, RED AND SOFT; ON SECTION EXUDES A DARK RED FLUID (CAP 18); REGIONAL LN (INGUINAL) NOT IDENTIFIED KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL SKIN (SK) : -EROSION,-MODERATE, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -FIBROSIS,-MODERATELY SEVERE, FOCAL -GALACTOCELE,-SEVERE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, FOCAL 4792 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 624 ANIMAL NUMBER: C92441 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 450.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM -RED FOCUS(I)/AREA(S); LEFT AND RIGHT ASPECT: SINGLE DARK RED AREA ON EACH, EACH IS 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4793 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 625 ANIMAL NUMBER: C92441 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 450.3 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 9:46 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY) 4794 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 626 ANIMAL NUMBER: C92442 SEX: FEMALE DATE OF DEATH: 07/25/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/25/99 10:27 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 386.7 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; YELLOW HAIR COAT; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; MILD; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE RED AREA, 3 X 2 MM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 9 X 9 MM, RED, DARK RED, GRAY, TAN AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN ERODED AREAS, UP TO 3 X 2 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL THYROID (TY) : -B-ADENOMA, C-CELL,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -EROSION,-SLIGHT, FOCAL -INFLAMMATION, ACUTE,-MINIMAL, DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOCAL 4795 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 627 ANIMAL NUMBER: C92442 SEX: FEMALE DATE OF DEATH: 07/25/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/25/99 10:27 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 386.7 GRAMS PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS: DRY, YELLOW MATERIAL; POSSIBLY CONSISTENT WITH STAINS (HAIRCOAT-YELLOW) AS NOTED IN CLINICAL OBSERVATIONS -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL; POSSIBLY CONSISTENT WITH STAINS (HEAD-RED) AS NOTED IN CLINICAL OBSERVATIONS -DISCHARGE-NASAL; DRY, RED MATERIAL 4796 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 628 ANIMAL NUMBER: C92442 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/25/99 STUDY DAY OF DEATH: 476 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 386.7 GRAMS DATE AND TIME OF NECROPSY: 07/25/99 10:27 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), MAMMARY, FEMALE (MF) 4797 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 629 ANIMAL NUMBER: C92443 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:15 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.1 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RED FOCUS(I)/AREA(S); ALL LOBES: MULTIPLE DARK RED AREAS, UP TO 7 X 9 MM; COLLECTED ON ROUTINE SECTIONS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 X 6 X 7 MM (RIGHT IS 3 X 4 X 5 MM) PITUITARY (PI) : -MASS(ES); SINGLE MASS, 7 X 7 X 10 MM, FIRM, RED, DARK RED AND TAN HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL LIVER (LI) : -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, FOCAL -THROMBUS,-MINIMAL, FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4798 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 630 ANIMAL NUMBER: C92443 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 593.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:15 PROSECTOR: DENNIS HOFFMAN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), URINARY BLADDER (UB) 4799 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 631 ANIMAL NUMBER: C92444 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:33 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); INGUINAL REGION-LEFT; WEEK 80; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 92; 35 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 101; 55 X 50MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; PERINEAL AREA; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 55 X 40 X 21 MM, RED, TAN, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; LEFT INGUINAL REGION: SINGLE MASS, 30 X 20 X 15 MM, DARK RED, TAN, AND LOBULATED; SOME SECTIONS EXUDE A DARK BROWN SEMIFLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT MUSCLE, SKELETAL (SM) : -I-LEIOMYOSARCOMA,-NEOPLASM PRESENT 4800 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 632 ANIMAL NUMBER: C92444 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:33 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MUSCLE, SKELETAL (SM) : -MASS(ES); SURROUNDS RECTUM AND BASE OF TAIL: SINGLE MASS, 50 X 45 X 25 MM, TAN, DARK RED, LOBULATED AND FIRM; SAME ON CUT SURFACE; POSSIBLY CONSISTENT WITH MASS (VENTRAL ABDOMINAL RIGHT) AS NOTED IN CLINICAL OBSERVATIONS; PORTION COLLECTED WITH RECTUM; REMAINING PORTION COLLECTED WITH TAIL; REGIONAL LN (LUMBAR) (CAP 22) PITUITARY (PI) : -LARGE; 6 X 4 X 3 MM OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 4 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT RECTUM (RE) : -M-LEIOMYOSARCOMA,-NEOPLASM PRESENT OVARY (OV) : >UNREMARKABLE DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4801 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 633 ANIMAL NUMBER: C92444 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 424.2 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:33 PROSECTOR: TONY HANFELD RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, LUMBAR/ILIAC (LM) 4802 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 634 ANIMAL NUMBER: C92445 SEX: FEMALE DATE OF DEATH: 01/31/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 01/31/00 14:50 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 795.2 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: TONE EVERTS PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; OBESE; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -MOTTLED; ALL LOBES: RED AND TAN BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, TAN, GRAY, AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; WITHIN LUMEN: LIGHT GREEN MUCOID SEMIFLUID GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MOIST MATERIAL YELLOW HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4803 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 635 ANIMAL NUMBER: C92445 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/31/00 STUDY DAY OF DEATH: 666 STUDY WEEK OF DEATH: 96 TERMINAL BODY WEIGHT: 795.2 GRAMS DATE AND TIME OF NECROPSY: 01/31/00 14:50 PROSECTOR: STEVE VAN ADESTINE RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL OBESE -DISCHARGE-NASAL; DARK RED DRY CRUSTY MATERIAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOt /f OOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY) 4804 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 636 ANIMAL NUMBER: C92446 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 328.5 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; VENTRAL-ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; NO FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 10 X 8 MM, RED, GREY, TAN AND SOFT STOMACH, NONGL (SU) : -EROSION/ULCERATION; MUCOSA: MULTIPLE TAN AND SLIGHTLY ERODED AREAS, UP TO 2 X 1 MM GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -PYELONEPHRITIS,-SLIGHT, FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,MODERATELY SEVERE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, NONGL (SU) : -ACANTHOSIS,-MODERATE, DIFFUSE -EDEMA,-MODERATE, FOCAL -EROSION,-MINIMAL, FOCAL -HYPERKERATOSIS, NONGLANDULAR MUCOSA,MODERATE, DIFFUSE -INFLAMMATION, CHRONIC-ACTIVE,-MINIMAL, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4805 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 637 ANIMAL NUMBER: C92446 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/05/99 STUDY DAY OF DEATH: 579 STUDY WEEK OF DEATH: 83 TERMINAL BODY WEIGHT: 328.5 GRAMS DATE AND TIME OF NECROPSY: 11/05/99 13:38 PROSECTOR: JOHN S. HALFORD RECORDER: JOHN S. HALFORD POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -STAINS-PERINEUM/PERIANAL; BOTH REGIONS, EXTENDING TO VENTRAL ABDOMINAL REGION: MOIST, BROWN MATERIAL -DISCHARGE-OCULAR; BILATERAL: DRY, RED MATERIAL -DISCHARGE-NASAL; DRY, RED MATERIAL -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (MF), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY) 4806 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 638 ANIMAL NUMBER: C92447 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:27 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; COLD TO TOUCH; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -GRANULAR MATERIAL; BILATERAL, WITHIN PELVIS: MULTIPLE TAN AND FIRM GRANULES, UP TO 1 MM IN DIAMETER -DIFFUSELY LIGHT; BILATERAL: TAN LIVER (LI) : -DIFFUSELY LIGHT; ALL LOBES: TAN BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, RED, TAN, AND SOFT STOMACH, GL (ST) : -DARK FOCUS(I)/AREA(S); MUCOSA: MULTIPLE BROWN FOCI, UP TO 1 MM IN DIAMETER HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LIVER (LI) : >UNREMARKABLE MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4807 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 639 ANIMAL NUMBER: C92447 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 01/03/00 STUDY DAY OF DEATH: 638 STUDY WEEK OF DEATH: 92 TERMINAL BODY WEIGHT: 294.4 GRAMS DATE AND TIME OF NECROPSY: 01/03/00 8:27 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY) 4808 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 640 ANIMAL NUMBER: C92448 SEX: FEMALE DATE OF DEATH: 08/02/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 08/02/99 14:43 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 362.2 GRAMS PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; MALOCCLUSION; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) THYMUS (TH) : -LARGE; 6 X 12 X 13 MM PITUITARY (PI) : -MASS(ES); SINGLE MASS, 6 X 7 X 12 MM, FIRM, RED, DARK RED, TAN AND BROWN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -MALOCCLUSION; BILATERAL UPPER INCISORS HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT THYMUS (TH) : -M-FIBROSARCOMA,-NEOPLASM PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4809 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 641 ANIMAL NUMBER: C92448 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 08/02/99 STUDY DAY OF DEATH: 484 STUDY WEEK OF DEATH: 70 TERMINAL BODY WEIGHT: 362.2 GRAMS DATE AND TIME OF NECROPSY: 08/02/99 14:43 PROSECTOR: DENNIS HOFFMAN RECORDER: DENNIS HOFFMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (MF), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) 4810 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 642 ANIMAL NUMBER: C92449 SEX: FEMALE DATE OF DEATH: 04/07/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/07/99 8:46 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 356.6 GRAMS PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT' (%) LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) .0453 .0417 2.0299 1.1844 1.1588 10.6589 1.4643 .0787 .0768 .7269 .0205 .0152 .8613 .0127 % .0117 % .5692 % .3321 % .3250 % 2.9890 % .4106 % .0221 % .0215 % .2038 % .0057 % .0043 % .2415 % LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY ORGAN TO BRAIN WEIGHT RATIO .0223 .0205 1.0000 .5835 .5709 5.2509 .7214 .0388 .0378 .3581 .0101 .0075 .4243 ORGAN S T A rr U S WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN WEIGHT TAKEN HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION PITUITARY (PI) : -LARGE; 6 X 5 X 3 MM; APPEARS SWOLLEN -DIFFUSELY RED; DARK RED GENERAL COMMENT (GC) : -BONE MARROW SMEAR TAKEN MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4811 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 643 ANIMAL NUMBER: C92449 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, THIRD INTERIM SACRIFICE DATE OF DEATH: 04/07/99 STUDY DAY OF DEATH: 367 STUDY WEEK OF DEATH: 53 TERMINAL BODY WEIGHT: 356.6 GRAMS DATE AND TIME OF NECROPSY: 04/07/99 8:46 PROSECTOR: JAMES T. CALLEN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: JARED FISHEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY) 4812 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 644 ANIMAL NUMBER: C92450 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 272.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 84; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL; WEEK 75; 20 X 20MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRALCERVICAL; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -CYST(S); LEFT LATERAL LOBE: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 1 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION MAMMARY, FEMALE (MF) : -MASS(ES); MID CERVICAL REGION: SINGLE MASS, 40 X 30 X 15 MM, YELLOW, TAN, LOBULATED, AND SOFT; CUT SURFACE CONTAINS A CREAMY YELLOW SEMISOLID; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL; COLLECTED -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 40 X 25 X 15 MM, TAN, DARK RED, AND SOFT; CUT SURFACE CONTAINS A TAN CREAMY SEMISOLID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL LIVER (LI) : ^UNREMARKABLE PANCREAS (PA) : -ATROPHY, ACINAR,-SLIGHT, MULTI-FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT -M-CARCINOMA,-NEOPLASM PRESENT 4813 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 645 ANIMAL NUMBER: C92450 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 272.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY MAMMARY, FEMALE (MF) : -MASS #3; LEFT INGUINAL REGION: SINGLE MASS, 30 X 25 X 25 MM, RED, TAN, AND FIRM; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 8 MM, DARK RED, RED, GRAY, AND SOFT UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 8 MM IN DIAMETER -THICKENED WALL; BILATERAL, DIFFUSE OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -ANIMAL THIN BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SEVERE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, FOCAL -SCLEROSIS,-MODERATELY SEVERE, MULTI FOCAL OVARY (OV) : >UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4814 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 646 ANIMAL NUMBER: C92450 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 12/15/99 STUDY DAY OF DEATH: 619 STUDY WEEK OF DEATH: 89 TERMINAL BODY WEIGHT: 272.2 GRAMS DATE AND TIME OF NECROPSY: 12/15/99 14:08 PROSECTOR: WENDY CHERRY RECORDER: WENDY CHERRY POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), LN, MANDIBULAR (MN) 4815 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 647 ANIMAL NUMBER: C92451 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 517.1 GRAMS PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; SWOLLEN; PAWS-HIND; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DARK FOCUS(I)/AREA(S); RIGHT MEDIAN LOBE: TWO BROWN AREAS, EACH IS 3 MM IN DIAMETER; COLLECTED ON ROUTINE SECTIONS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) SPLEEN (SP) : -LARGE; 50 X 16 X 10 MM ADRENAL, CORTEX (AC) : -LARGE; RIGHT: 10 X 7 X 7 MM (LEFT IS 6 X 4 X 4 MM) HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION, VASCULAR,-MINIMAL, MULTI-FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT SPLEEN (SP) : -HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED,-MINIMAL, MULTI-FOCAL ADRENAL, CORTEX (AC) : -ANGIECTASIS,-MODERATE, MULTI-FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL 4816 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 648 ANIMAL NUMBER: C92451 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 10:06 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 517.1 GRAMS PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 7 X 6 X 4 MM, DARK RED, TAN AND SOFT UTERUS (UT) : -CYST(S); BILATERAL, WITHIN WALLS: SINGLE CLEAR FLUID FILLED CYST ON EACH, LEFT IS 10 X 7 X 7 MM, RIGHT IS 15 X 10 X 10 MM JOINT, OTHER (JT) : -LARGE; BILATERAL, TARSAL JOINT: EACH IS UP TO 18 MM IN DIAMETER; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN ADRENAL, MEDULLA (MA) : -NECROSIS, MEDULLARY,-SLIGHT, MULTI FOCAL -B-PHEOCHROMOCYTOMA,-NEOPLASM PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT UTERUS (UT) : -DILATATION,-SEVERE, DIFFUSE DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT JOINT, OTHER (JT) : -EXOSTOSES,-MODERATELY SEVERE, MULTI FOCAL -FIBROSIS,-MODERATELY SEVERE, MULTI FOCAL -INFLAMMATION, CHRONIC, SYNOVIAL,SLIGHT, MULTI-FOCAL 4817 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 649 ANIMAL NUMBER: C92451 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 517.1 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 10:06 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB) 4818 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 650 ANIMAL NUMBER: C92452 SEX: FEMALE DATE OF DEATH: 04/04/00 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/04/00 8:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.5 GRAMS PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -DARK FOCUS(I)/AREA(S); LEFT MEDIAN LOBE: SINGLE DARK BROWN AREA, 3 MM IN DIAMETER (CAP 22) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 7 X 6 X 6 MM, DARK RED, TAN, AND SOFT STOMACH, GL (ST) : -THICKENED WALL; DIFFUSE OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 2 MM IN DIAMETER CERVIX (CV) : -LARGE; 1 0 X 1 0 X 8 MM HISTOPATHOLOGY LUNG (LU) : -MINERALIZATION,-MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, FOCAL LIVER (LI) : -TELANGIECTASIS,-MINIMAL, FOCAL MULTI BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT STOMACH, GL (ST) : -EDEMA,-MINIMAL, MULTI-FOCAL OVARY (OV) : >UNREMARKABLE CERVIX (CV) : -HYPERTROPHY, MUSCULAR,-PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT FOOT/FOOTPAD (FP) : -EROSION,-MODERATE, FOCAL 4819 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 651 ANIMAL NUMBER: C92452 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 477.5 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 8:28 PROSECTOR: WENDY CHERRY RECORDER: PAMELA L. SCHULZ-STUEBER POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY FOOT/FOOTPAD (FP) : -ABRASION(S)/ULCERATION(S); RIGHT HINDFOOT: SINGLE DARK RED ABRADED AREA, 10 X 6 X 5 MM; COLLECTED GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN FOOT/FOOTPAD (FP) : -INFLAMMATION, CHRONIC ACTIVE,MODERATE, MULTI-FOCAL -PIGMENT,-MINIMAL, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF), URINARY BLADDER (UB) 4820 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 652 ANIMAL NUMBER: C92453 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 396.5 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 80; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: NOT OBSERVED >SKIN & PELAGE; RED HAIR COAT; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: NOT OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT CERVICAL REGION, EXTENDING TO RIGHT AXILLARY REGION: SINGLE MASS, 38 X 32 X 20 MM, FIRM, LOBULATED, RED, AND TAN; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 14 X 9 X 6 MM, FIRM, LOBULATED, RED, DARK RED, AND TAN GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4821 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 653 ANIMAL NUMBER: C92453 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 396.5 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:59 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, AXILLARY (AX) 4822 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 654 ANIMAL NUMBER: C92454 SEX: FEMALE DATE OF DEATH: 10/23/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 10/23/99 11:03 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 310.8 GRAMS PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-CERVICAL; WEEK 54; 40 X 40MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); VENTRAL-ABDOMINAL-RIGHT; WEEK 61; 25 X 25MM; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; SORE/SCAB; VENTRAL ABDOMINAL; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; SQUINTED; PERI-ORBITAL; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT INGUINAL REGION: SINGLE MASS, 25 X 20 X 10 MM, TAN AND FIRM; SAME ON CUT SURFACE; OVERLYING SKIN HAS A SINGLE DARK RED, DRY, CRUSTED AREA, 3 X 2 MM; MASS AND OVERLYING SKIN COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED -MASS #2; RIGHT VENTRAL CERVICAL REGION: SINGLE MASS, 45 X 30 X 25 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (MANDIBULAR) NORMAL (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 15 X 10 X 10 MM, RED, TAN, GRAY, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4823 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 655 ANIMAL NUMBER: C92454 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 10/23/99 STUDY DAY OF DEATH: 566 STUDY WEEK OF DEATH: 81 TERMINAL BODY WEIGHT: 310.8 GRAMS DATE AND TIME OF NECROPSY: 10/23/99 11:03 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), LN, MANDIBULAR (MN) 4824 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 656 ANIMAL NUMBER: C92455 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 430.3 GRAMS DATE AND TIME OF NECROPSY: 03/23/00 10:53 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; RED HAIR COAT; HEADENTIRE; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 101; 45 X 40MM; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; RED HAIR COAT; SHOULDERLEFT; VERIFIED AT NECROPSY: NOT OBSERVED >APPEARANCE; HEAD TILT; LEFT; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY THYROID (TY) : -DIFFUSELY RED; LEFT: DARK RED MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN CREAMY SEMIFLUID FILLED CYSTS, UP TO 40 X 25 X 19 MM (CAP 21); POSSIBLY CONSISTENT WITH (MASS AXILLARY) NOTED IN CLINICAL OBSERVATIONS BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 12 X 6 X 5 MM, RED, DARK RED, TAN, SOFT AND LOBULATED HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL LIVER (LI) : -HYPERPLASIA, BILE DUCT,-MINIMAL, MULTI FOCAL -INFLAMMATION, CHRONIC,-MINIMAL, MULTI FOCAL THYROID (TY) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA, C-CELL,- 2 NEOPLASMS PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -MINERALIZATION,-MINIMAL, FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -B-ADENOMA,-NEOPLASM PRESENT 4825 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 657 ANIMAL NUMBER: C92455 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 03/23/00 STUDY DAY OF DEATH: 718 STUDY WEEK OF DEATH: 103 TERMINAL BODY WEIGHT: 430.3 GRAMS DATE AND TIME OF NECROPSY: 03/23/00 10:53 PROSECTOR: JILL PAUS RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY OVARY (OV) : -CYST(S); RIGHT: SINGLE CLEAR FLUID FILLED CYST, 3 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; LEFT: DARK RED, DRY MATERIAL OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA) 4826 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 658 ANIMAL NUMBER: C92456 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:34 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 88; 45 X 40MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY LIVER (LI) : -RAISED FOCUS(I)/AREA(S); ALONG MARGIN OF LEFT LATERAL LOBE: SINGLE TAN RAISED AREA, 4 X 3 X 1 MM (CAP 23) MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 40 X 40 X 22 MM, RED, TAN, YELLOW, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (AXILLARY) NORMAL (CAP 22) GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -BONE MARROW SMEAR TAKEN HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL -NEPHROPATHY, CHRONIC PROGRESSIVE,MINIMAL, MULTI-FOCAL LIVER (LI) : -FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC,-MODERATE, FOCAL -FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC,-MINIMAL, FOCAL -THROMBUS,-MODERATELY SEVERE, FOCAL MAMMARY, FEMALE (MF) : -B-FIBROADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -SCHEDULED SACRIFICE,-PRESENT 4827 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 659 ANIMAL NUMBER: C92456 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, TERMINAL SACRIFICE DATE OF DEATH: 04/04/00 STUDY DAY OF DEATH: 730 STUDY WEEK OF DEATH: 105 TERMINAL BODY WEIGHT: 523.3 GRAMS DATE AND TIME OF NECROPSY: 04/04/00 11:34 PROSECTOR: JODI THIEREN RECORDER: JILL PAUS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), PANCREAS (PA), THYROID (TY), URINARY BLADDER (UB), LN, AXILLARY (AX) 4828 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 660 ANIMAL NUMBER: C92457 SEX: FEMALE DATE OF DEATH: 11/15/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 11/15/99 13:28 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 333.2 GRAMS PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; HEAD TILT; RIGHT; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: NOT OBSERVED >MASS(ES); AXILLARY REGION-LEFT; WEEK 84; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT AXILLARY REGION: SINGLE MASS, 12 X 9 X 8 MM, TAN AND FIRM; SAME ON CUT SURFACE (CAP 18); REGIONAL LN (AXILLARY) NORMAL (CAP 19) -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE WHITE FLUID FILLED CYSTS, UP TO 3 X 2 X 2 MM; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) ADRENAL, CORTEX (AC) : -LARGE; LEFT: 6 MM IN DIAMETER (RIGHT IS 4 MM IN DIAMETER) PITUITARY (PI) : -MASS(ES); 12 X 8 X 7 MM, DARK RED, RED, GRAY, TAN AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATE, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT ADRENAL, CORTEX (AC) : -DEGENERATION, CYSTIC, CORTICAL,MODERATELY SEVERE, MULTI-FOCAL -HEMORRHAGE,-SLIGHT, MULTI-FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATELY SEVERE, MULTI FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4829 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 661 ANIMAL NUMBER: C92457 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 11/15/99 STUDY DAY OF DEATH: 589 STUDY WEEK OF DEATH: 85 TERMINAL BODY WEIGHT: 333.2 GRAMS DATE AND TIME OF NECROPSY: 11/15/99 13:28 PROSECTOR: JERALD W. JOHNSON RECORDER: JERALD W. JOHNSON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY UTERUS (UT) : -CYST(S); BILATERAL, WITHIN LUMEN: MULTIPLE CLEAR FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -STAINS-PERINEUM/PERIANAL; MATERIAL DRY, BROWN UTERUS (UT) : -HYPERPLASIA, CYSTIC, ENDOMETRIAL,MODERATE, MULTI-FOCAL DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), ADRENAL, MEDULLA (MA), LN, AXILLARY (AX) 4830 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 662 ANIMAL NUMBER: C92458 SEX: FEMALE DATE OF DEATH: 07/19/99 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/19/99 8:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 254.2 GRAMS PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; BROWN HAIR COAT; PERINEAL AREA; VERIFIED AT NECROPSY: NOT OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; RECUMBENT; LATERAL; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY LIVER (LI) : -LIGHT FOCUS(I)/AREA(S); DIAPHRAGMATIC SURFACE OF RIGHT MEDIAN LOBE: SINGLE TAN AREA, 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 9 X 5 MM, RED, TAN, LOBULATED, AND SOFT GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: DARK RED, DRY MATERIAL HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, ALVEOLAR,MINIMAL, FOCAL KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-SLIGHT, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4831 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 663 ANIMAL NUMBER: C92458 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 07/19/99 STUDY DAY OF DEATH: 470 STUDY WEEK OF DEATH: 68 TERMINAL BODY WEIGHT: 254.2 GRAMS DATE AND TIME OF NECROPSY: 07/19/99 8:37 PROSECTOR: LYNN HORNER RECORDER: STEVE VAN ADESTINE POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -ANIMAL THIN THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) (CE), THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: PANCREAS (PA), THYROID (TY), MAMMARY, FEMALE (MF) 4832 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 664 ANIMAL NUMBER: C92459 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 292.3 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:28 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); AXILLARY REGION-RIGHT; WEEK 70; 35 X 35MM; VERIFIED AT NECROPSY: OBSERVED >MASS(ES); INGUINAL REGION-RIGHT; WEEK 92; 30 X 30MM; VERIFIED AT NECROPSY: OBSERVED OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); RIGHT AXILLARY REGION: SINGLE MASS, 35 X 35 X 25 MM, RED, TAN, FIRM, AND LOBULATED; SAME ON CUT SURFACE; COLLECTED; REGIONAL LN (SEE AXILLARY) -MASS #2; RIGHT INGUINAL REGION: SINGLE MASS, 55 X 34 X 22 MM, RED, DARK RED, TAN, BROWN, SOFT, AND LOBULATED; ON SECTION SOME AREAS CONTAIN A BROWN FLUID; COLLECTED; REGIONAL LN (INGUINAL) NOT IDENTIFIED BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) HISTOPATHOLOGY LUNG (LU) : -INFILTRATE, MACROPHAGE, PIGMENTED,MINIMAL, FOCAL KIDNEY (KD) : -MINERALIZATION, PELVIC,-MINIMAL, MULTI FOCAL THYROID (TY) : -HYPERPLASIA, FOLLICULAR CELL,MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MODERATELY SEVERE, MULTI FOCAL -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-FIBROADENOMA,- 2 NEOPLASMS PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT 4833 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 665 ANIMAL NUMBER: C92459 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/28/00 STUDY DAY OF DEATH: 694 STUDY WEEK OF DEATH: 100 TERMINAL BODY WEIGHT: 292.3 GRAMS DATE AND TIME OF NECROPSY: 02/28/00 13:28 PROSECTOR: DENNIS HOFFMAN RECORDER: TONE EVERTS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY PITUITARY (PI) : -MASS(ES); 10 X 8 X 6 MM, RED, DARK RED, TAN, AND SOFT LN, AXILLARY (AX) : -DIFFUSELY RED; RIGHT: GENERAL COMMENT (GC) : -BLOOD FILM TAKEN (CAP 19) PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT LN, AXILLARY (AX) : -HEMORRHAGE,-MINIMAL, MULTI-FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LIVER (LI), PANCREAS (PA) 4834 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 666 ANIMAL NUMBER: C92460 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 679 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 289.2 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 8:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >MASS(ES); VENTRAL-ABDOMINAL; WEEK 62; 40 X 35MM; VERIFIED AT NECROPSY: OBSERVED >EYES; SQUINTED; PERI-ORBITAL-LEFT; VERIFIED AT NECROPSY: NOT APPLICABLE >SKIN & PELAGE; SORE/SCAB; PAW-HINDRIGHT; VERIFIED AT NECROPSY: OBSERVED >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; ATAXIC; VERIFIED AT NECROPSY: NOT APPLICABLE >APPEARANCE; HUNCHED; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -MASS(ES); LEFT PERINEAL REGION: SINGLE MASS, 37 X 25 X 15 MM, TAN, VASCULAR, LOBULATED AND SOFT; ON SECTION EXUDES A TAN FLUID (CAP 21); REGIONAL LN (LUMBAR) NORMAL (CAP 20) -THICKENED; VENTRAL CERVICAL REGION: TAN AND FIRM (CAP 19) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 11 X 9 X 9 MM, RED, TAN, GRAY AND SOFT OVARY (OV) : -CYST(S); LEFT: SINGLE CLEAR FLUID FILLED CYST, 6 MM IN DIAMETER FOOT/FOOTPAD (FP) : -CRUSTED AREA(S); RIGHT HIND FOOT: SINGLE RED, TAN AND DRY AREA, 5 X 4 MM; COLLECTED HISTOPATHOLOGY THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -MINERALIZATION,-MINIMAL, MULTI-FOCAL -B-FIBROADENOMA,-NEOPLASM PRESENT -M-CARCINOMA,-NEOPLASM PRESENT BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -HEMORRHAGE,-SLIGHT, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT OVARY (OV) : -CYST,-PRESENT DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT FOOT/FOOTPAD (FP) : -EDEMA,-MODERATE, MULTI-FOCAL -EROSION,-MODERATE, MULTI-FOCAL 4835 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 667 ANIMAL NUMBER: C92460 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/13/00 STUDY DAY OF DEATH: 679 STUDY WEEK OF DEATH: 97 TERMINAL BODY WEIGHT: 289.2 GRAMS DATE AND TIME OF NECROPSY: 02/13/00 8:33 PROSECTOR: GREGORY CODDINGTON RECORDER: GREGORY CODDINGTON POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; AND DRY -ANIMAL THIN : BILATERAL: DARK RED FOOT/FOOTPAD (FP) : -INFLAMMATION, ACUTE,-MODERATE, MULTI FOCAL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), LN, LUMBAR/ILIAC (LM) 4836 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 668 ANIMAL NUMBER: C92461 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/04/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 371.4 GRAMS DATE AND TIME OF NECROPSY: 06/03/99 7:57 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >DISCHARGE; NASAL; RED IN COLOR; VERIFIED AT NECROPSY: OBSERVED >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >SKIN & PELAGE; PALE; ENTIRE BODY; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; SENSITIVE TO TOUCH; VERIFIED AT NECROPSY: NOT APPLICABLE >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY KIDNEY (KD) : -LARGE PELVIS(ES); RIGHT -GRANULAR MATERIAL; BILATERAL, WITHIN PELVES: TAN GRANULAR MATERIAL; COLLECTED INTACT MAMMARY, FEMALE (MF) : -CYST(S); ALL REGIONS, DIFFUSE: MULTIPLE TAN AND CREAMY FLUID FILLED CYSTS, UP TO 4 MM IN DIAMETER; COLLECTED ON ROUTINE SECTION -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM; COLLECTED ON ROUTINE SECTION BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 10 X 8 X 7 MM, RED, DARK RED, TAN AND SOFT HISTOPATHOLOGY KIDNEY (KD) : -DILATATION, PELVIC,-PRESENT -MINERALIZATION, PELVIC,-MODERATE, MULTI-FOCAL LIVER (LI) : -VACUOLATION, HEPATOCELLULAR,-MINIMAL, MULTI-FOCAL PANCREAS (PA) : -ATROPHY, ACINAR,-MINIMAL, MULTI-FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-MINIMAL, MULTI-FOCAL BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT PITUITARY (PI) : -CYST,-PRESENT -HEMORRHAGE,-MINIMAL, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT 4837 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 669 ANIMAL NUMBER: C92461 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 06/04/99 STUDY DAY OF DEATH: 425 STUDY WEEK OF DEATH: 61 TERMINAL BODY WEIGHT: 371.4 GRAMS DATE AND TIME OF NECROPSY: 06/03/99 7:57 PROSECTOR: TERRI L. WILLIAMS RECORDER: TERRI L. WILLIAMS POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) : -BLOOD FILM TAKEN -DISCHARGE-OCULAR; BILATERAL: RED -DISCHARGE-NASAL; BILATERAL: RED DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), THYROID (TY) 4838 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 670 ANIMAL NUMBER: C92462 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 279.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE *** ORGAN WEIGHTS WERE NOT RECORDED *** LAST CLINICAL OBSERVATIONS >APPEARANCE; THIN; VERIFIED AT NECROPSY: OBSERVED >EXCRETION; FEW FECES; VERIFIED AT NECROPSY: NOT APPLICABLE >EYES; RED DISCHARGE; EYES; VERIFIED AT NECROPSY: OBSERVED >BEHAVIOR; HYPOACTIVE; VERIFIED AT NECROPSY: NOT APPLICABLE OBSERVATIONS NECROPSY MAMMARY, FEMALE (MF) : -THICKENED; ALL REGIONS, DIFFUSE: TAN AND FIRM (CAP 18) BRAIN (BR) : -COMPRESSED (HYPOTHALAMUS) PITUITARY (PI) : -MASS(ES); 13 X 8 X 8 MM, DARK RED, GRAY, AND SOFT VAGINA (VA) : -SEMIFLUID MATERIAL; LIGHT GREEN AND MUCOID HISTOPATHOLOGY KIDNEY (KD) : -CAST, PROTEINACEOUS,-MINIMAL, MULTI FOCAL -MINERALIZATION, PELVIC,-SLIGHT, MULTI FOCAL THYROID (TY) : -CYST, ULTIMOBRANCHIAL,-PRESENT -HYPERPLASIA, C-CELL,-MODERATE, FOCAL MAMMARY, FEMALE (MF) : -GALACTOCELE,-SLIGHT, MULTI-FOCAL -HYPERPLASIA, GLANDULAR,-MODERATELY SEVERE, DIFFUSE BRAIN (BR) : -COMPRESSION, VENTRAL,-PRESENT -DILATATION, VENTRICULAR,-PRESENT -HEMORRHAGE,-MODERATE, FOCAL -PIGMENT,-SLIGHT, FOCAL PITUITARY (PI) : -HEMORRHAGE,-MODERATE, MULTI-FOCAL -B-ADENOMA,-NEOPLASM PRESENT VAGINA (VA) : ^UNREMARKABLE DEATH COMMENT (DC) : -PITUITARY ADENOMA,-PRESENT 4839 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 671 ANIMAL NUMBER: C92462 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: UNSCHEDULED (M) DATE OF DEATH: 02/21/00 STUDY DAY OF DEATH: 687 STUDY WEEK OF DEATH: 99 TERMINAL BODY WEIGHT: 279.2 GRAMS DATE AND TIME OF NECROPSY: 02/21/00 13:21 PROSECTOR: NANCY DIEDRICH RECORDER: NANCY DIEDRICH POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: NOT AVAILABLE LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY (CONTINUED) HISTOPATHOLOGY GENERAL COMMENT (GC) -BLOOD FILM TAKEN -DISCHARGE-OCULAR; AND CRUSTED -ANIMAL THIN : BILATERAL: DARK RED THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUm /s KULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) THE FOLLOWING TISSUES WERE UNREMARKABLE AT MICROSCOPIC EXAMINATION: LUNG (LU), LIVER (LI), PANCREAS (PA) 4840 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 672 ANIMAL NUMBER: C92463 SEX: FEMALE DATE OF DEATH: 04/28/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/28/98 8:26 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 238.0 GRAMS PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.5785 -------------------------------------------------- ------------------------------------ 3.1842 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4841 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 673 ANIMAL NUMBER: C92463 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 238.0 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:26 PROSECTOR: STEVE VAN ADESTINE RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4842 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 674 ANIMAL NUMBER: C92464 SEX: FEMALE DATE OF DEATH: 07/07/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/07/98 8:37 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 240.5 GRAMS PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.7170 -------------------------------------------------- ------------------------------------ 3.2087 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4843 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 675 ANIMAL NUMBER: C92464 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 240.5 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:37 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4844 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 676 ANIMAL NUMBER: C92465 SEX: FEMALE DATE OF DEATH: 07/07/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/07/98 8:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.6 GRAMS PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.6100 -------------------------------------------------- ------------------------------------ 2.6460 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4845 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 677 ANIMAL NUMBER: C92465 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 287.6 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:23 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4846 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 678 ANIMAL NUMBER: C92466 SEX: FEMALE DATE OF DEATH: 07/07/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/07/98 8:23 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 267.4 GRAMS PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.5092 -------------------------------------------------- ------------------------------------ 2.8082 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4847 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 679 ANIMAL NUMBER: C92466 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 267.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:23 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4848 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 680 ANIMAL NUMBER: C92467 SEX: FEMALE DATE OF DEATH: 04/28/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 04/28/98 8:33 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 205.2 GRAMS PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 8.2470 -------------------------------------------------- ------------------------------------ 4.0190 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4849 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 681 ANIMAL NUMBER: C92467 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 205.2 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:33 PROSECTOR: TERRI L. WILLIAMS RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4850 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 682 ANIMAL NUMBER: C92468 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 249.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:01 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.7884 -------------------------------------------------- ------------------------------------ 3.1229 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4851 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 683 ANIMAL NUMBER: C92468 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 249.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 9:01 PROSECTOR: LYNN HORNER RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4852 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 684 ANIMAL NUMBER: C92469 SEX: FEMALE DATE OF DEATH: 07/07/98 STUDY DAY OF DATE AND TIME OF NECROPSY: 07/07/98 8:42 POST-FIX WEIGHER: NOT AVAILABLE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.4 GRAMS PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.3542 -------------------------------------------------- ------------------------------------ 3.2058 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; VERIFIED AT NECROPSY: OBSERVED GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4853 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 685 ANIMAL NUMBER: C92469 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, SECOND INTERIM SACRIFICE DATE OF DEATH: 07/07/98 STUDY DAY OF DEATH: 93 STUDY WEEK OF DEATH: 14 TERMINAL BODY WEIGHT: 229.4 GRAMS DATE AND TIME OF NECROPSY: 07/07/98 8:42 PROSECTOR: JODI THIEREN RECORDER: MATTHEW AXTMAN POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: AS ASSIGNED WEIGHER: DEBBIE PIRKEL THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4854 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 686 ANIMAL NUMBER: C92470 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 204.6 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:24 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER ORGAN NAME ABSOLUTE ORGAN WEIGHT (GRAMS) ORGAN WEIGHT RELATIVE TO BODY WEIGHT (%) ORGAN TO BRAIN WEIGHT RATIO ORGAN STATUS LEFT ADRENAL (AD0) RIGHT ADRENAL (AD1) BRAIN (BR) LEFT KIDNEY (KD0) RIGHT KIDNEY (KD1) LIVER (LI) LUNG (LU) LEFT OVARY (OV0) RIGHT OVARY (OV1) SPLEEN (SP) LF THYROID/PARA (TT0) RT THYROID/PARA (TT1) UTERUS (UT) ------------------------------------ 7.3676 -------------------------------------------------- ------------------------------------ 3.6010 % -------------------------------------------------- -------------------------------------------------------------------------------------------- NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN WEIGHT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN NOT TAKEN LAST CLINICAL OBSERVATIONS OBSERVATIONS NECROPSY HISTOPATHOLOGY >NORMAL; NO REMARKABLE OBSERVATIONS; UNVERIFIED AT NECROPSY GENERAL COMMENT (GC) : -NO MACROSCOPIC LESIONS 4855 Covance 6329-212 APPENDIX 6 Individual Anatomic Pathology Data Covance 6329-228 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS PAGE: 687 ANIMAL NUMBER: C92470 SEX: FEMALE DOSE GROUP: 9 SACRIFICE STATUS: SCHEDULED, FIRST INTERIM SACRIFICE DATE OF DEATH: 04/28/98 STUDY DAY OF DEATH: 23 STUDY WEEK OF DEATH: 4 TERMINAL BODY WEIGHT: 204.6 GRAMS DATE AND TIME OF NECROPSY: 04/28/98 8:24 PROSECTOR: MICHELE MARGGI RECORDER: DEBBIE PIRKEL POST-FIX WEIGHER: NOT AVAILABLE PATHOLOGIST: DR. JAMES L. CARTER WEIGHER: TROY ELLENBECKER THE FOLLOWING ORGANS WERE UNREMARKABLE AT NECROPSY: LUNG (LU), KIDNEY (KD), LIVER (LI), PANCREAS (PA), THYROID (TY), PARATHYROID (PT), SKIN (SK), MAMMARY, FEMALE (MF), BONE, FEMUR (FE), MARROW, FEMUR (FM), MARROW, STERNUM (SE), BONE, STERNUM (SB), EYE (EY), BRAIN (BR), SPINAL CORD (SC), MUSCLE, SKELETAL (SM), NERVE, SCIATIC (SN), HEART (HT), LN, MESENTERIC (MS), SPLEEN (SP), THYMUS (TH), ESOPHAGUS (ES), TRACHEA (TR), ADRENAL, CORTEX (AC), ADRENAL, MEDULLA (MA), PITUITARY (PI), HARDERIAN GLAND (HG), SALIV GL, MANDIB (SG), STOMACH, GL (ST), STOMACH, NONGL (SU), DUODENUM (DU), JEJUNUM (JE), ILEUM (IL), CECUM (CE), COLON (CO), RECTUM (RE), URINARY BLADDER (UB), UTERUS (UT), OVARY (OV), CERVIX (CV), VAGINA (VA), LN, MANDIBULAR (MN), LN, AXILLARY (AX), FOOT/FOOTPAD (FP), LN, INGUINAL (IN), AORTA (AO), LN, RENAL (RL), LN, LUMBAR/ILIAC (LM), CAVITY, ABDOM (PC), TAIL (TI), URETER (UE), CAVITY, ORAL (OC), CALVARIUM/SKULL (CA), LN, MEDIASTINAL (ML), LN, OTHER (LN), BILE DUCT (BD), AUDITORY SEB GL (AS), LN, ANT MES/PANC (AP), ADIPOSE TISSUE (AT), JOINT, OTHER (JT), LN, POPLITEAL (PP), ADRENAL (AD) 4856 APPENDIX 7 C e ll P ro life ra tio n R e p o rt Covance 6329-212 Covance 6329-228 3M T-6316.1 4857 Covance 6329-212 Covance 6329-228 3M T-6316.1 4858 Covance 6329-212 Covance 6329-228 3M T-6316.1 4859 TABLE OF CONTENTS Report Narrative Table Figure Histopathology Findings Signature Page Quality Assurance Statement Covance 6329-212 Covance 6329-228 3M T-6316.1 Section I II III IV V VI 4860 Covance 6329-212 Covance 6329-228 3M T-6316.1 I. REPORT NARRATIVE 4861 Covance 6329-212 Covance 6329-228 3M T-6316.1 3M Covance Study No. 6329-228 Amended Final Cell Proliferation Report CELL PROLIFERATION REPORT FOR: 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS COVANCE STUDY NUMBER 6329-228 INTRODUCTION The purpose of the study was to assess the carcinogenicity of the test material when adm inistered in the diet to rats for at least 104 weeks. This report, subm itted by Pathology Associates - A Charles River Company [formerly Pathology Associates International (PAI)] to the study Sponsor, 3M Toxicology Services, represents the cell proliferation findings and interpretation for Covance Study N um ber 6329-228 entitled " 104W eek Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonam ido Ethanol In Rats". REGULATORY COMPLIANCE A ll aspects o f the tasks associated w ith P A I's portion o f this study w ere conducted in com pliance with the Food and Drug Adm inistration Good Laboratory Practice (GLP) Regulations as set forth in T itle 21 o f the US C ode o f Federal R egulations, Part 58, issued D ecem ber 22, 1978 (effective June 20, 1979), and with any applicable amendments. RECORD RETENTION PCNA evaluation data, paraffin blocks and tissue slides are retained by PAI. MATERIALS AND METHODS Tissue Collection for Cell Proliferation Five anim als per sex per group in groups 8 (control) and 9 (treated) were sacrificed during week 14 for hepato cellu lar proliferation rate m easurem ents. A section o f the left lateral lobe of the liver from each of the 20 animals was fixed and processed to paraffin block by Covance per protocol specifications. Tissue blocks were shipped to PA I for sectioning and staining. From each block, a slide was prepared for H& E evaluation and im munohistochemical detection of proliferating cell nuclear antigen (PCNA), a marker of cell proliferation. Page 1-1 4862 Covance 6329-212 Covance 6329-228 3M T-6316.1 3M Covance Study No. 6329-228 Amended Final Cell Proliferation Report Immunohistochemistry for Cell Proliferation Sections of paraffin-em bedded tissues were cut = 5 pm and placed on positively charged slides (Superfrost Plus, Fisher Scientific, Pittsburgh, PA) to ensure adhesion during processing for PCNA. Standard im m unohistochem ical methods for PCNA (Eldridge, et ah, 1993) were used to stain tissues. Briefly, tissue sections were incubated with a monoclonal antibody to PCN A (Lot# 016 Exp. 10/99 DAKO, Carpinteria, CA) and reagents required for the avidin-biotin peroxidase (ABC Elite Kit K327 Lot# PK-6100 Exp. 2/10/99, Vector, Burlingame, CA) m ethod for the detection of the antigen-antibody com plex. PC N A expression in cells in all phases of the cell cycle (G l, S, G2 and M ) was localized by the chrom agen 3,3'-diam inobenzidine (DAB Lot# 18H8201 E xp.4/00, Sigm a Chem ical Co., St. Louis, M O). Tissue sections were counterstained with hem atoxylin. Cell Proliferation Measurements Positive staining for PCN A was categorized based on cellular distribution and intensity of the brown to black reaction product that correlated with the different phases of the cell cycle (Eldridge et ah, 1993). Uniform, dark brown to black nuclear staining was judged to be positive for S phase cells. A cell in the G, phase of the cell cycle had diffuse, stippled nuclear staining that was lighter than a cell in S phase. Cells in the G 2 phase exhibited distinct, diffuse cytoplasm ic stippling with or without nuclear staining. M itotic figures were conspicuous with diffuse cytoplasm ic stippling. The percentage of proliferating hepatocytes (proliferating index PI; G j+ S + G a M phases com bined) was determ ined by scoring at least 3000 hepatocytes in 10 random ly selected fields per animal. A negative control slide was included in the staining run and consisted of study tissue (animal no. C92255) that was not incubated with the primary antibody. For cell proliferation evaluations, slides were first perused at low m agnification (100X) to judge quality of staining, processing and sectioning, pattern of cell labeling (e.g., centrilobular or panlobular, extrahepatocellular proliferation, such as Kupffer cells, bile duct epithelium , endothelium ), and histom orphologic changes. The later was further assessed by evaluating the serial H & E slide for each animal evaluated for cell proliferation. Cell proliferation was then quantified at higher m agnification (200X) as described above. Statistical Analysis The Student's t-test (two-sided, unequal variance) was used to test for statistical significance in PI betw een control and treatm ent groups using M icrosoft Excel version 5.0. A P value of less than 0.05 was judged to be statistically significant. Page 1-2 4863 Covance 6329-212 Covance 6329-228 3M T-6316.1 3M Covance Study No. 6329-228 Am ended Final Cell Proliferation Report RESULTS Cell Proliferation Individual anim al and group m ean cell proliferation data are presented in Section II (Table II - 1) and graphically in Figure III-1. The percentage o f proliferating hepatocytes (PI) did not differ significantly in m ale or fem ale rats exposed to the 1 ppm N EtFO SE at 14 weeks on study. Histopathology Sections from the same tissue blocks used for preparation of PCNA-stained slides were stained with hem atoxylin and eosin (H&E) for histopathologic evaluation to facilitate the interpretation of the im m unostained slides. Individual animal findings are presented in Section IV. S ingle sections o f liver from 10 m ale and 10 fem ale rats representing 5 anim als/treatm ent group/sex including control and 1 ppm NEtFOSE treatment groups were evaluated histologically. Each tissue section was stained with hematoxylin and eosin. The purpose of this evaluation was to determ ine w hether or not m orphologic changes were occurring in these tissues that may alter or confound the specificity of the PCNA staining observed in these animals. The results showed that no significant changes that would alter the interpretation of the PCNA staining in this study were observed in the liver tissues from either the m ale or fem ale rats. DISCUSSION In the present study, cell proliferation was m easured within the liver of male and female rats from control and 1 ppm N EtFO SE after 14 weeks on study. A statistically significant increase in cell proliferation, as determ ined by the proliferating index (PI) was not observed in male or fem ale rats from the 1 ppm N E tFO SE group after 14 w eeks on study. F urtherm ore, there w as no indication of a biologically relevant proliferative response. SUMMARY A fter 14 w eeks on study, enhanced cell proliferation w as not evident in the liver o f m ale and females rats exposed to 1 ppm NEtFOSE. LITERATURE CITED Eldridge, S.R., Butterw orth, B.E., Goldsworthy, T.L. (1993). Proliferating cell nuclear antigen: A m arker for hepatocellular proliferation in rodents. E n v i r o n H e a l t h P e r s p e c 101: 211-218. Page 1-3 4864 Covance 6329-212 Covance 6329-228 3M T-6316.1 II. TABLE 4865 Covance 6329-212 Covance 6329-228 3M T-6316.1 3M C o v a n c e Study No. 6329-228 TABLE II-1, CELL PROLIFERATION IN LIVER OF RATS AFTER 14 WEEKS O N STUDY Dose Group 8 - 0 p p m (C ontrol) 8 - 0 p p m (C ontrol) 8 - 0 p p m (C ontrol) 8 - 0 p p m (C ontrol) 8 - 0 p p m (C ontrol) 9 - 1 p p m NEtFOSE 9 - 1 p p m NEtFOSE 9 - 1 p p m NEtFOSE 9 - 1 p p m NEtFOSE 9 - 1 p p m NEtFOSE 8 - 0 p p m (C ontrol) 8 - 0 p p m (C ontrol) 8 - 0 p p m (C ontrol) 8 - 0 p p m (C ontrol) 8 - 0 p p m (C ontrol) 9 - 1 p p m NEtFOSE 9 - 1 p p m NEtFOSE 9 - 1 p p m NEtFOSE 9 - 1 p p m NEtFOSE 9 - 1 p p m NEtFOSE Proliferating A nim al Index Sex Number Liver M C92255 0.173% M C92256 0,199% M C92257 0.029% M C92258 0.147% M C92260 0.265% Mean 0.163% SEM 0.039% M C92325 0.1 18% M C92326 0.146% M C92327 0.1 70% M C92328 0.087% M C92330 0.030% Mean 0.110% SEM 0.025% t-test 0.2928 F C92396 0.058% F C92397 0.544% F C92398 0.147% F C92399 0.269% F C92400 0.205% Mean 0.245% SEM 0.083% F C92464 0.266% F C92465 0.089% F C92466 0.295% F C92468 0.435% F C 92469 0.261% Mean 0.269% SEM 0.055% t-test 0.8116 Page II-1 4866 Covance 6329-212 Covance 6329-228 3M T-6316.1 III. FIGURE 4867 PROLIFERATING INDEX (%) Covance 6329-212 Covance 6329-228 3M T-6316.1 4868 Covance 6329-212 Covance 6329-228 3M T-6316.1 IV. HISTOPATHOLOGY FINDINGS 4869 Covance 6329-212 Covance 6329-228 3M T-6316.1 3M Covance Study No. 6329-228 Individual Animal Histopathology Findings 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Histopathologic Findings after 14 weeks on Study Tissue: Liver ANIMAL NUMBER SEX DOSE GROUP HISTOLOGIC FINDINGS C92255 C92256 C92257 C92258 C92260 C92325 C92326 C92327 C92328 C92330 M 8-0 ppm (Control) M 8-0 ppm (Control) M 8-0 ppm (Control) M 8-0 ppm (Control) M 8-0 ppm (Control) M 9-lppm NEtFOSE M 9-lppm NEtFOSE M 9-lppm NEtFOSE M 9-lppm NEtFOSE M 9-lppm NEtFOSE No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings C92396 C92397 C92398 C92399 C92400 C92464 C92465 C92466 C92468 C92469 F 8-0 ppm (Control) F 8-0 ppm (Control) F 8-0 ppm (Control) F 8-0 ppm (Control) F 8-0 ppm (Control) F 9-lppm NEtFOSE F 9-lppm NEtFOSE F 9-lppm NEtFOSE F 9-lppm NEtFOSE F 9-lppm NEtFOSE No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings No Significant Findings Page IV-1 4870 Covance 6329-212 Covance 6329-228 3M T-6316.1 4871 Covance 6329-212 Covance 6329-228 3M T-6316.1 VI. QUALITY ASSURANCE STATEMENT 4872 Covance 6329-212 Covance 6329-228 3M T-6316.1 4873