Document dgnn10MyeEKGM5NKygLDZ1eB

AR226-2978 Du Pont H LR 477-92 T P A P J ...5 .Q BII Study Tftlo STATIC, ACUTE, 96-HOUR LC50 O F H-19,173 T O FATHEAD MINNOWS, Pimephates pmmelas Data Requirement United States Environmental Protection Agency Environmental Effects Test Guidelines Subdivision EG-9 Author Kevin N. Baer, Ph.D. Study CompSeted.Qn September 24,1932 Performing Laboratory E. I. du Pont de Nemours and Company Haskeii Laboratory tor Toxicology and Industrial Medicine Eikton Road, Post Office Box 50 Newark, Delaware 19714 laboratory Project IP Haskeii Laboratory Report No. 477-92 Company Sanitized. Does not contain TSCfi Page 1 of 14 Du Pont HLR 477-92 G O O D LABO RATO RY PRACTICE STATEM ENT the test substance In dilution water and under storage conditions were not determined. Concentrations of the test substance in dilution water were not measured. Protocol amendments and/or S O P deviations appear in Appendix I Areas of noncompliance (if any) are also documented in the study records. No deviations existed that affected the scientific validity of the study. Submitter: E. I. du Pont de Nemours and Company Sponsor: Du Pont Chem ica's E. I. du Pont de Nemours and Company - STUDY DIRECTOR: Kevin N. Baer, Ph.D. Research Toxicologist Environmental Sciences Mo/Day/Year Company Sanitized. Does not contain TSCA CBf 2 nnnnmm Du Pont HLR 477-92 TABLE OF CONTENTS Page G ood Laboratory Practice Statem ent...................................... ............... ............ .. .. 2 Table of Contents............................. ............................. ....... ..................................3 G eneral Information.................................................................................................... .. Sum m ary...... ............... ................... ............ ................................................ ........,, 5 Q uality Assurance Docum entation.......................... 6 L P u rp o se ................... 7 It R e co rd s...................................... ,..,,...7 i l l M aterials and Methods A Test Substance..... B. Dilution W ater........ C. Anim al C u ltu re ...... D. Test M ethods......... E Chem ical A n a ly s is . IV. Results and D iscussion...... .................. . ............................ ......................... 8 V. C o n c lu s io n .................................... ............................................... . . . . . . . . 9 VI. Literature C ite d ............................. ...................... ...................................... 9 VII. T ab les........................................................................................................ .. 1. Chem ical Characteristics of Haskell Laboratory W ell W ater..................10 2. W ater Chem istry of the Dilution W ater at Test S ta rt...............................11 3. Physical and Chem ical Characteristics of H-19,173 Test Solutions . .. .. 1 2 4. O bserved Mortality of Fathead Minnows. Pim ephales prom elas, Exposed to H-19,173 for 96 Hours in a Static, Unaerated T e st............. 13 VIII. A p p e n d ix ........ .................................................. .................................. 14 1. Protocol Amendments and S O P Deviations..........................................14 UfSMpsiif tS itM tiid . IRiei n(A confain 're ** F j-T Da Pont H L R 477-92 G EN ER A L INFO RM ATIO N 0 ..... ..... Purity: H -1 9,173 i Du Pont Chem icals E. I. du Pont de Nemours and Company Wilmington, Delaware 19898 4-1-92/9-24-92 4-2-92/6-21-92 rSanitized. Does not contain TSCA CBS 4 Do Pont HLR 477-92 TSOTAFTAITCH, AECAUDTMEI.N9N6O-HWOSU, RPLimCe5p0hOalFesHp.m19m.1e7l3as SUM M ARY H-19.173 exhibited low toxicity in a 96-hour, unaerated, static, acute test using fathead minnows, Pim ephales prom slas. Nominal H 19.173 concentrations were 2 4 0 ,3 4 0 ,4 9 0 ,7 0 0 , and 1000 m g/L However, undissolved test substance was observed in ail test solutions. The 96-hour LC50, based on nominal concentrations w as greater than 1060 mg/L, the highest nominal concentration tested. Due to the lack of solubility, Stability, and analytical confirmation of the concentration of the test substance in dilution water, the nominal LC50 should be interpreted with caution. Work By: {Jeffrey T. Turner, S r/ Aquatic Associate fhy/fe Mo/Day/Year Work By: Aquatic Associate THIS REPO RT IS APPR O VED FO R ISSUE. Research Toxicologist and Study Director Environmental Sciences *7fz fM z Mo/Day/Year 1P P 5FShltlzeci. OssiWo!contata TSCACl! Du Pont HLR 477-92 C U A LITY A SSU R A N C E DOCUM ENTATION (H-19173) nates of Inspection: Conduct-4/3; 6/17/92 Records, Reports) - 9/17 -18/92 Data Findings reported ta: Study Director - 9/21/92 Management - 9/24/92 Reported by Donna R. Holt Quality Assurance Auditor Du Pont HLR 477-92 I. P U R P O S E The goaf of this study was to assess the acute toxicity of H-19,173 to fathead minnows, Pim ephales prom elas. II. R E C O R D S A il data and the final report wilt be maintained either at Haskell Laboratory for Toxicology and Industrial Medicine (Newark, Delaware) or be archived at the Du Pont Records Management Center (Wilmington, Delaware). III. M A T E R IA LS AN D M ETH O D S A . Test Substanc* The test substance is described under Gene al Information. Test solutions were prepared by pipetting the appropriate quantity of H-19,173 and adding it to dilution water (see below). Stability and solubility of H-19,173 in dilution water and under storage conditions were not determined. B. Dilution Water W ater (Table 1) originated from the Haskell Laboratory well. C . Animal Culture Fathead Minnows, Pim ephales promelas, were reared in continuously flowing well water at Haskell Laboratory. Adult fish resided in 29 L aquaria at approximately 21 C and were fed frozen brine shrimp fArtemia sp.. Golden G ate Brand, Kordon Division, Novalek, Inc., Hayward, CA) generally twice daily, once on Saturday and once on Sunday. Eggs were deposited on polyvinyl chloride tiles coated with sand. Tiles with eggs were aerated and incubated at approximately 25C in a water bath. Larvae were reared in 29 L aquaria at approximately 22C and were fed newly hatched Artemia sp. (Aquarium Products, Glen Burnie, MD) generally twice daily on weekdays and daily on weekends. Juveniles were held either in 272-L circular, fiberglass tanks (23-cm depth; 50 x 25 x 31-cm diameter) or in 29 L glass aquaria at approximately 21 C and were fed newly hatched Artemia sd. and/or frozen brine shrimp generally twice daily on weekdays, once on Saturday and once on Sunday. Sickness, injury, and abnormality were not observed. Animals were identified by labels on aquaria and test vessels. 7 Du Pont HLR 477-92 D . T e st Methods [1] Five test concentrations and a water control were used. Nominal concentrations were 240,340,490.700, and 1000 mg/L. Control fish ranged from 1.2 to 1.8 cm standard length (mean 1.5 cm) and 0.020 to 0.070 g wet weight (mean 0.044 g). G la ss aouaria [20 L; 5 0 (L )x 2 5 (W )x 31 cm (H)] containing 15 L of trot solution (approximately 19-cm liquid depth) were employed. Ten fish were added to each replicate using random numbers (two replicate per concentration, total 20 fish per roncentration). Control loading was 0.029 g/L at test conclusion Test solutions ranged from 21.1 to 21.6C (mean 21.3C). Fish were not fed 48 hourspnor to nor during the test. A photoperiod of 16 hours light versus 8 ^ da^ f ^ ftwas em ployed with 25 minutes of transitional light preceding and foHowing the 16 hour light interval. Mortality and behavioral effects were noted daily. D issolved oxygen (YSt Model 54A, Yellow Springs Instruments, Yellow Springs, OH), pH (Com ing Model 610A, Corning Scientific>Instalments, Medfield, MA), and temperature (American Society of Testing Matenals registered mercury thermometer) were measured in the water rontrol and an test concentrations before fish rare added at the beginning of the test and d a iy thereafter. Total alkalinity (Hach kit, Hach Chem ical Company, Loveland, CO), E D T A hardness (Hach kit, Hach Chem ical Company, Loveland, CO ), and conductivity (YSI M odel 33, YeHow Springs Instruments, Yellow Spnngs. OH) of the dilution water were m easured at the beginning of the test. A continuously-recording thermometer in a control vessel was used to check tor temperature variation during the 96-hour test. E . C h em ical A n a ly s is Concentrations of H-19,173 in test solutions were not measured. IV. R E S U LT S A N D D ISCUSSIO N A 96-hour rangefinding test was conducted using nominal concentrations of 1.0, 10,100, and 1000 mg/L (five fish per concentration). No mortalities were observed at any test concentration. A previous definitive study was conducted but was repeated due to excessive control mortality. Ait chem ical and physical parameters in the definitive test were within expected ranges (Tables 2 and 3). Nominal concentrations were 240,340, 490,700, and 1000 mg/L. However, undissolved test substance was observed nail test solutions. The 96-hour LC50, based on nominal concentrations and mortality (Table 4) was greater than 1000 mg/L, the highest nominal concentration tested. Due to the lack of solubility, stability, and analytical confirmation of the concentration of the test substance in dilution water, the nominal LC50 should be interpreted with caution. COW*** 8 Du Pont HLR <77-92 V. CO N CLU SIO N H-19,173 (undissolved as tested and based on nominal concentrations) exhibited low toxicity in a 96-hour, unaerated, static, adute test Rising fathead minnows, Pim aphales promaSas. The 96-hour LC50, based on iilm in a i concentrations was greater than 1000 mg/L, the highest concentration tested. VI. LIT E R A T U R E CITED 1. United States Environmental Protection Agency, Office of Toxic Substances, Guidelines for Testing Chem icals E6-9, Washington, D.C., August 1982. co^ nv^ Hotcontain TSCA CB1 - 0005 9 Du Pont HLR 477-92 TSOTAFTAITCH, EAACDUTMEI,N9N6O-HWOSU, RPiLmCe5p0haOleFsHp-r1o9m,1o7te3s TABLE 1 Chem ical Characteristics of Haskell Laboratory W eii W ater Param eter Concentration Param eter Concentration BOD, mg/L CO D , mg/L DO C. mg/L T O C 21, mg/L Total Kjeidahl N. mg/L Am m onia N, mg/L Turbidity, NTU Phenolics, mg/L Color, Co/Pt S o lid s Total suspended, mg/L Aluminum, mg/L Antimony, mg/L Arsenic, mg/L Beryllium, mg/L Cadmium, mg/L Calcium , mg/L Chloride3- mg/L Chromium, mg/L Cobalt, mg/L Copper, mg/L Cyanide, mg/L Iron3, mg/L F lu o rid e 3 <2 <10 2.8 2.9 <0.2 <0.05 1.6 <0.050 <5 <4 <0.100 <0.060 <0.010 <0.001 <0.002 24.5 6.2 <0.010 <0.020 <0.010 <0.025 0.025 <0.1 Lead, mg/L Magnesium, mg/L M BAS/LAS, mg/L Mercury, mg/L Nickel, mg/L ' Nitrite3, mg/L Nitrate3, mg/L Phosphate3, mg/L Potassium, mg/L Selenium , mg/L Silver, mg/L Sodium, mg/L Sulfate3, mg/L Sulfide, mg/L Thallium, mg/L Z in c 4,* mg/L Volatile priority pollutants Acid extractable priority pollutants Base/neutral priority pollutants P e s tic id e s /P C B s Organophosphate pesticides, pg/L <0.075 3.8 <0.05 <0.00020 <<<20001.....1110820 <<800...0001005 4.8 <<<000...0001250 ND6 ND6 ND ND6 <0.50 1Date of sam ple collection 30 December 1981 unless indicated otherwise, analyses performed at Environmental Testing and Certification Corporation. Edison, New Jersey; 2Date of sample collection 17 June 1992, performed at Du Pont Engineering Test Center, Newark, Delaware; 3Date of analyses 13 January 1992, analyses performed at Du Pont Chem icals, Jackson Laboratory, Deepwater, New Jersey; 4Below the Published Method Detection Limit (BMDL); ^Analyses performed at Hazleton Laboratories, Inc., Madison, Wl; None detected. , n Cfflp*ySaniti*-Ooes no*containTSCACB Du Font HLR 477-92 TSOTAFTAITCH, EAACDUTMEI,N9N6O-HWOSU, RPiLmGe5p0hOaleFsHp-r1o9m,1e7la3s TABLE 2 W ater Chemistry of the Dilution W ater at Test Start Total Alkalinity (mg/L as CaCO s) 85 EDTA Hardness (mg/L as C a C 03) 78 Conductivity (umhos/cm) 170 Coinp30^SamAxed Does not con'" tSCACBt 11 (Days) Controi A* Ba STATIC, ACUTE, 96-HOUR LC50 O F H-19.173 TO FATHEAD MINNOWS, Pim aphales promelas TABLE 3 Physical and Chemical Characteristics of H-19,173 Test Solutions Nominal Concentrations (mg/L) 240 AB 340 AB 490 *70( ABA 1000 AB Du Pont H L R 477-92 Company Sanitized. Does not Dissolved Oxygen (mg/L) 0 8.6 8.6 8.6 8.6 1 8.6 8.4 8.5 8.6 2 8.5 8.4 8.5 8.5 3 8.4 8.4 8.4 8.4 4 8.4 8.4 8.4 8.4 pH 0 7.4 7.4 7.4 7.4 1 7.7 7.7 7.7 7.7 2 7.8 7.8 7.8 7.8 3 7.8 7.8 7.9 7.9 4 7.8 7.8 7.8 7.9 Temperature (C) 21.50 2 1 .6 2 1 .6 2 1 .6 21.31 2 1 . 2 2 1 .2 2 1 .2 2 2 1 .1 2 1 .2 2 1 .1 2 1.1 3 21.32 1 . 2 2 1 .2 2 1 .2 4 21.42 1 . 2 2 1 .2 2 1 .2 aA and B represent replicates. 8.6 8.6 8.4 8.5 8.4 8.4 8.4 8.4 8.4 8.4 7.4 7.4 7.7 7.7 7.8 7.8 7.9 7.8 7.8 7.8 21.5 2 1 .2 2 1 .1 2 1 .1 2 1 .1 2 1 .6 2 1 .2 2 1 .2 2 1 .1 2 1 .1 8.6 8.6 8.6 8.5 8.5 8.4 8.4 8.4 8.4 8.3 7.4 7.4 7.7 7.7 7.8 7.8 7.8 7.9 7.8 7.8 2 1 .6 2 1 .2 2 1 .1 21.5 21.4 2 1 .6 21.3 2 1 .1 21.3 21.3 8.6 8.6 8.6 8.6 8.5 8.4 8.4 8.4 8.4 8.4 8.6 8.6 8.6 8.5 8.4 8.4 8.4 8.4 8.3 8.3 7.4 7.4 7.7 7.7 7.8 7.8 7.8 7.8 7.8 7.8 7.4 7.4 7.7 7.7 7.8 7.8 7.9 7.9 7.9 7.,, 21.5 2 1 .2 2 1 .1 21.3 21.4 21.5 2 1 .2 2 1 .1 21.4 21.4 21.5 21.5 2 1 .2 2 1 .1 22A11A..54A 2 1 .2 A4 4 AJ A2 1 . 1 2A2114 ..34A Du Pont HLR 477-92 TSOTAFTAITCH. EAACDUTMEI,N9N6O-HWOSU. RPiLmCe5p0haOleFsHp-r1o9m.1e7la3s TABLE 4 Observed Mortality of Fathead Minnows, Pim ephales promelas, Exposed to H-19,173 for 96 Hours In a Static. Unaerated Test Nom inal C o n c e n tra tio n s (mg/L) H 20 Control 240 340 490 700 1000 24 Hours AB 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 Cumulative Mortality in Percent 48 Hours AB 72 Hours AB 0/10 0/10 0/10- 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 /10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 96 Hours AB 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 A and B represent replicates; each replicate contained 10 fish {total 20 fish per test concentration). notcontain TSCA CB1 Company Sanitized. Ooes 13 Du Pont H L R 477-92 STATIC, ACUTE. 96-HOUR LC50 O F H-19.173 TO FATHEAD MINNOWS, Pim ephales prom elas APPENDIX 1 PR O TO CO L AM ENDM ENTS AND S O P DEVIATIONS PROTOCOL AMENDMENTS 1. Analytical sam ples will not be taken. R EASO N F O R CH AN G E: Sponsor request. 2. The Sponsor's approval date is 8-17-91. ___ R EASO N F O R CH ANG E: Date inadvertently omitted in the S T S PS. S O P DEVIATIONS 1. T O C samples were inadvertently not taken in the first definitive test. 2. The photoperiod (Lux) was inadvertently not measured in the second definitive. r,tteed.t>oesno Company San 14