Document daxp3rd61moN2aZ67526bw5a9

Submitted to; rC -tZ -% AR:O-02i? BIOSEARCH, INC. p.o. box 8598 Philadelphia, Pennsylvania 19 10 1 telephone: (215) 848-4499 Project Number - 77"1127A 3M Company 3M Center S t . Paul , Minnesota 55101 Material: Sample Received: Date of Report: Test; Object of Test: Procedure: 3M Company - T-2001CoC 11/21/77 Study Initiated: 11/23/77 Study Completed: 12/29/77 1/6/78 Acute Oral Toxicity - Rats To study the acute oral toxicity in rats of the subject material. Four groups of 5 male 6 5 female albino rats of the Sherman-Wistar Strain weighing between 200 and 300 gm were employed in this study. The rats were deprived of food but not water for 2k hours prior to dosing. Each animal was weighed and dosed by direct administration of the experimental material into the stomach by means of a syringe and dosing needle. The sample was dosed as supplied. The following dosage levels were administered: 0.50 ml/kg. 2.50 ml/kg. 3-75 ml/kg. 5.00 ml/kg. Resu1ts: Conclus ion: Following administration the animals were allowed food and water ad libitum for the H day observation period during which time the rats were observed for signs of toxicity and mortalities. See Table 1. The subject material when studied in male and female albino rats has an acute oral LD50 between 0.5 ml/kg. and 2.5 ml/kg. 000182 Karl L. Gabriel, V.M.D., Ph.D. Di rector TABLE 1 Acute Oral Toxicity 000183 Material: 3M Company - T-2001CoC, as supplied. Dosage Level ml/kg 0.50 0.50 2.50 2.50 3.75 3.75 5.00 5.00 Number of Animals Dosed 5 males 5 females 5 males 5 females 5 males 5 females 5 males 5 females Morta 1it ies Days 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0000 00000 0 0 0 0 0 Total Dead 14 Days 0 Total Survived 14 Days 5 000000000 0 0 0 0 0 0 5 3 10 1 23 50 50 3 1000 1 50 41 50 5 50 41 50 The LD50 's between 0.5 ml/kg. and 2.5 ml/kg. At 0.50 ml/kg. the animals were slightly depressed for 4-6 hours after dosing. At the three higher dosage levels the animals were comatose within 30 minutes to 4 hours and died as noted. Gross pathologic examination revealed nothing remarkable. Initial Weight gm 215 230 225 250 210 235 220 2*0 Final We ight gm 245 255 - - - - -