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AR ARE - O750 ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: ArefmeirxrteudretocaonstaPiFnOiSng, pFeCr-f9i5uo,roorocatsanaescuolmfponoanteen,twhoficFhC-m2a0y3CalEs.o b(1eOctanesulfonic acid) (CAS # 2795-39-3). R2e0m3aCrEks(2:121/8T9h)e. 3CMurrperondtuicntfioornmaltoitonnuimnbdeircawteassit5i1s9a. Thetestsample mixture of 1.84% is FC- PfoFaOmSe,r,330.%1%diseotdhyiluemneocgtlyylcosullfbauttey,l 2et.h1e%r,p6o0l.yo2x3y%etwhaytleern,e2m.o5n6o%ocStuylltpohneenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. cThhaerafcotlelorwiiznegd scounmcmenatrryataipopnlsioefs tiompaurmiitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical component.ofthe test sample. METHOD: Method: OECD Method 203 and U.S. EPA Method EG-9 TGyLpPe:: NAocute static renewal YSpeeacriceosm:plPeitmeedp:hal1e9s89promelas SAnuaplpyltiiecra:l AmqounaittiocriBinog:SyTsetmepmesr,atInucr.e,FoprHt,CoalnldinDs,OCO EStxaptoisstuircael pmeertihodo:ds9:6-Nhooturgsiven. TLeesntgtfihshanadgew:eiJguhvte:niles AAvveerraaggee lweeniggthht== 109..096mgm(wet) Loading: 0.20 g fishL PTreesttrceoantdmietnito:nsN:one DDiilluuttiioonn wwaatteerr:chCeamrbiosnt-rfyi:ltered well water Temperature: 20C DO: 96 mglL pH: 80 Lighting: Not given. tSo ttohcekdainludtitoenswtastoelruotinoan wperiegphatr/avtoilounm:e Tbhaseist.est material was added directly -- CStoanbcielnittyraotfitohnestdesotscihnegmriactae:l sDoaliultyions: Not noted Exposure vessels: 4 L Pyrex glass cylindrical tanks containing 3 Lof solution. Number of replicates: 2 001094 Number Number of of fish per replicate: 10 concentrations: one plus a blank control Water chemistry during the study: pH ran7g.e9(80-.906 (hcoounrtsro)l:exposure) Temper7a.t9u-re8.r0a(n1g0e00(0m-g9/6Lheoxupross):ure2)0-21C Dissolved oxygen range (0-96 hours): 7.79.6 mg/L (control exposure) 3.89.6 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Bk control and 1000 mg/L. Element values: 96-hour LC50 =>1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test tShuebsftluaonrcoechReemmicaarlkpsrfoipeolrd.tiTonhealvoanle.ues reported apply to that mixture and not CONCLUSIONS The test sample 96-hour LCs for fathead minnow was determined to be >1000 mgiL. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliabilty: Kiimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amountoffluorochemical proportion in the solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G1368-1, 1989. OTHER Last changed: 6/26/00 001095 LEW LUNENIAL LABUKALUKT LRGphang/ ' DEFINITIVE (FULLSALSTEST) TYPETESTZiSl ted le JOHRMs ICAL TESTEDLCZASCE 2/20/89 Lom.s2 TEST ORGANISMEthernit) SOURCE Bysiogs a. TATEREC'D.G/LS/R9 BATCH 0.35/22 AVG. WT.Q.0u0200iSgraAnVsG.. SIZEL92Z%0ue cm" NO. OF OGRANISMS PER TREATMENT. LOADING FAC0.2T0 Og/R L FISH DISEASE TREATMENTARAE TEST VESSEL: Liter Byrerglasscylindricaltank TEST SOLUTION VOLUMES 1 DTIILMUETSITOANRTWEATDER9C:a0s0hoans:(filteredwDeAlTlE wSaTteArR(TTeEmDp._2.0S4C/,82DDA.T2ECO0MmPgL/.ElT,EDM__SA0S_/_A)Z NYSE EEGs 195% Confidence Linits) M0uple aot || RA RA TRA rani micial tora| 20 ours | soos | 22ers | o6tore | No.| Conc.| Amount] Total Ne. pH 0.0. [Total No] pH p.0. [Total No] pH [D.0. [Total No] pH P.O tr rr rrr rr rr Tr ctr rrr rrr T1111 COMMENTS#:1 44ida) weights, Test waterinl agoeavance (clear ue lou) Goa, quid; ; pn EFFECTS (OTHERFo SalI--p---- 1 MORTALITY) PY rr or + iiT Zr LL Tov T&F THY TQ] lz pp v p rr 1 "11] C1 1 11 Test methodology conformswith the folloying two test protocols: (8) ehTacsPriTessdclhySeasiv. BEST.COPY AVAILABLE 001096