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ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 1. The testsample isFC206. Currentinformationindicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether,78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate0,.04% sodium lauryslulfate0,.5% polyoxyethylene monooctylphenylether,and 0.05% benzotriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: Not given. Type: Acute statiacnd continuousflow GLP: No Year completed: 1974 Species: Pimephalespromelas Supplier:Not given. Analyticalmonitoring:Temperature,pH, and DO. Exposure period: 96-hours Statisticamlethods: LC50 valuecalculateudsinggraphicinterpolation. Test fishage: Juveniles Length and weight: Loading: Not given Average length 2 in. Average weight 1.6g (wet) Pretreatment:None Test conditions: Dilutionwater: Carbon-fllterceidtywater,St.Paul,MN Dilutionwater chemistry:Not given. Lighting:Not given. Stock and testsolutionpreparation:A 50% primarystocksolutiownas prepared forthe continuousflowstudy.The testmaterialwas added directly tothe dilutiownater on a volume/volume basisforthe staticstudy. DiluterFlow rate: Not given. Concentrations dosing rate: Continuous fortheflowstudy,once forthe staticstudy. Stabilityof the testchemical solutions: Not noted. Exposure vessels: Not given. Number of replicates:1 Number offishperreplicateN:otgiven. Number ofconcentrationfsi:veplusa blankcontrol Waterchemistryduringthestatisctudy: pH range(0-96hours): 6.9- 7.0(contreoxlposure) 7.1- 7.2 (1250 mg/L exposure)* Temperature range (0-96hours): 67 - 72 'F Dissolved oxygen range (0-96hours): 4.7- 5.7 mg/L (controelxposure) 3.9- 5.7 mg/L (1250 mg/L exposure)* 1250 mg/L (intermediatceoncentratione)xposure values used because the highestthreeconcentrationsresultedintotalmortalitbyy 72 hours. Water chemistry during the continuous flow study: pH range (0-96hours): 6.9- 7.0(controlexposure) 7.0- 7.1(2500 mg/L exposure)* Temperature range (0-96hours): 67 -72 'F Dissolved oxygen range (0-96hours): 6.0- 6.3 mg/L (controelxposure) 4.5- 6.0 mg/L (2500 mg/L exposure)* 2500 mg/L (intermediatceoncentratione)xposure values used because the highesttwo concentrationsresultedintotalmortalitbyy 48 hours. RESULTS Nominal concentrations: Blank control5,00, 1250, 2500, 3750, and 5000 mg/L Element values: 96-hour LC50 (static=) 1750 mg/L 96-hour LC50 (continuousflow)= 3000 mg/L Element valuesbased on nominal concentrations Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSTQNS The testsample 96-hour LC50 forfathead minnow was determined to be 1750 mg/L under staticonditionsand 3000 mg/L under continuousflowconditions. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking3. Testinglacksrecordofmethodology used and documentation. The sample puritywas not propedy characterizedand the studylacksanalyticaclonfirmatioonfthe amount offluorochemical proportioninthe solution. REFERENCES This studywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number 2083, 1974. OTHER Last changed: 6/27/00 FORM 10620-PWO cc:LAB REQUEST BOOK - 42-5w File: Water and Wastewater v@O REQUEST FOR LABORATORY WORK LabRequestNo. TO: ENVIRONMENTAL ENGINEERING LABORATORY - BUILDING 53-6N Requestor Lab Personnel Name: @@QLC.Qb Request Date Date Rec'd.Sample: ProjectNo. Description: Prob. No.2@7rF Code Analyst: m - y B p Sample Date: ate Needed: Date Completed: Hold Sample -,sEst.Time: Hrs. Hrs.Spent: @S3@Description Total Hardness,mg/l as CaC03 Calcium Hardness, mg/l as CaC03 "P" Alkalinitym,g/i asCaC03 "M" Alkalinitym,g/l asCaC03 pH Sulfate,mg/j as S04 Nitratem,g/las N03 Chloride,mg/l as Cl Silicam,g/i as Si02 Tluoride-m,g/i as F Ortho Phosphate,mg/l as P Total Phosphate,mg/i as P TotalChromium, mg/l asCr Hex. Chromium, mg/ias Cr TotalCyanide, mg/l as CN Ferrocyanide,mg/i as CN Iron,mg/i as Fe Manganese, mg/i as Mn Surfactant,mg/ias LAS Phenol,mg/i BOD, mg/i COD, mg/i (Short,Long,Dilute) Total Suspended Solids,mg/i VolatileSuspended Solids,mg/i Turbidity,mg/i Silica Total Solids,mg/l Total VolatileSolids,mg/i Conductivity,mhos Total Bacteria,No./100 mi TotalColiform,No./100 mi FecalColiform,No./100 ml FecalStreptococcus,No./100 mi Ammonia Nitrogen,mg/ias N KiedidahlNitrogen,mg/i as N Oil a!@dGrease, mg/l &/0 0-S.SOL q6 A6 Comments: o(o c I 1 1!70 1 14- 1 1 ol I White - Environmental Engrg. Lab Canary - Environmental Engrg. Lab Pl-nk - Environmental Engrg. Lab Goldenrod 3M awmemn ft Requntors Copy 740101 ENVIRONMEN'TAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET Type Test 'Fest organism Date Started l'imeStarted -- 6;@ DilutionWaterCit\t Mater'ialtested Avg. Weight cA Date Completed \n e-c, D.0 . ..C........o..n....c.. Survj%@,i] Pff pnm -T Control, -50oP/,C)/,y\co /c@0 1 0c) 1-@@/500 7 So Soo@ 50. :7z 48 hrs 72 hrs 96 hrs '0 D.0 % D.0 D.0 ;ur,,i-t,a I -PH ppm Sur%-ival pH pl)m Survival \oo \oo 5770 \00 oo !5,7 -4 loo '72IA,4-7 7.6 ------Averag_q 7'CM Iql@atllx_c. COM,Ments: -@2ZO SA-OCK Sc)ItA@i&V\ 7ol 0.o5- 5 0.,3757 190 740101 Type Test Test organism \@\n@kr Date Started Time Started Dilution Water ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY WORK SHEET io )CIV7 f, . Material tested Avg. Weight Date Completed CC)Coonnttrrool, 24'hrs % Survival 0 k0v 00v D.0 H m 7 pHLi 48 hrs oa% @Suruvrivviavlal D.0 pfl m 72 hrs oi6 - Survival D.0 H 'PPM 96 hrs % Survival D.0 PtI,] PPM \oo --Aoo C.) uk00 Ic 17715577 m -io @@0:cT)7I/- 5,75 al 0 -T oo 6c) Comir.ents: 70 7a cp Go fill 7%L I-T ttt-. 4A + 4 L T' + 4 t t '7 +4 + +f t I T 4@t t - -4t f @-T 4 io-i- - 4 4 t ..... ........ + . . . .. . . .. .. . :Tj t4. 4 T'rt ID 11 i'.*,4" 4- ti ++* CIC) N C, EIZ.L31 3N3on3 tt tit 77 0 (D P, -1 4+ 4+ 44 + l@n H3NI N3d SNCIISIAICI Ot X S3n",. z d3dVd Hdybig N30Z.L310 OLE-1-OVC 'ON