Document dYObqmgYEjBZeqrZo2jBwZLdb

Internal Correspondence de: M. T. Case - 218-3 E. G. Lamprecht - 218-3 W. C. McCormick - 220-2E-02 V. Pothapragada - 236-3A R. A. Prokop - 236-2B L. D. W inter - 236-2B To: E. G. GORTNER - RIKER - 218-3-3 From: T. R. MATHISEN - COMMERCIAL CHEMICALS - 236-2B Subject ANALYTICAL ANALYSES OF SUSPENSION OF FM-392 4 Dale: A p r il 8, 1981 INTRODUCTION* Reference: CCD A n a ly tic a l Method #G-29-0381 Four samples o f corn o i l w ith suspended FM-3924 were re ce ive d on March 16, 1981 from E. G. G o rtn e r. The fo u r samples la b e le d Placebo corn o i l , T-2999 COC 25 m g/kg/day FM-3924, T-2999 COC 2-5 mg/kg/day and T-2999 COC 0.25 m g/kg/day were subm itted f o r mg/ml c o n c e n tra tio n o f FM-3424, f o r use in a r a t fe e d ing s tu d y . The samples were run by a GLC method using a methanol e x tra c tio n and an in te r n a l sta n d a rd . GLC RESULTS: Sample Placebo Corn O il T-2999 COC FM-3924 0.25 mg/kg/day T-2999 COC FM-3924 2.5 mg/kg/day T-2999 COC FM-3924 25 m g/kg/day Mg/Ml FM-3924 No FM-3924 d e tected 0.0459 mg/ml FM-3924 0.4694 mg/ml FM-3924 5.136 mg/ml FM-3924 SUMMARY: A ll samples were w ith in 10% o f ta rg e t and showed good r e p r o d u c ib ilit y . The s o l u b i l i t y o f FM-3924 was found to be low. Standards used show signs o f s a tu ra tio n a t 0 .5 mg/ml le v e l. I t was found th a t s o n ific a tio n would d is s o lv e the FM-3924 in to the corn o i l . But a f t e r s e ve ra l days the 5 mg/ml standard showed some c r y s t a llz a t io n , b u t a f t e r s o n ific a tio n i t would r e - s o lu b iliz e . The c r y s ta liz a tio n could cause problems in the feeding study corn o i l . TRM/hc