Document dYMRrp2edK8zwjNDYkVO54726

AR226-2926 FOR DUPONT USE ONLY HASKELL LABORATORYREPORT NO. 180-85 Approximate Lethal Concentration by Inhalation (ALC? of Haskell E. I. du Pont de Nemours and Co., Inc. Laboratory for Toxicology and Industrial Elkton Road, P. 0. Box 50 Newark, Delaware 19714 Medicine ompany Sanitized. Does nol contain TSCA CB1 Date Issued: April 18, 1985 Approximate Lethal Concentration by Inhalation (ALC) of ;CP(SD)BR rats were exposed to dust atmospheres of ^^S^E1p^o--Sif--f^Wfourptoa single, 4ghour period. 2100 nig/in Under the No deaths conditions of . . s considered to have very low acute toxtcity by inhalation. ^^ ff'^^ .iL^ja Work by; f------^ '''^ Thomas A. Regelman Technician Supervised by: -^^_ .\^^ t> ^/(S/Z^ Laura A. Kinney? Chemist lpl>ro"edbl': ^-^sy^s. Study Director Section Supervisor Acute Investigations ^lrtrs Acknowledgements; Jruce A. Burgess also participated in this study. LAK:HLR 9.3 ^3,ne-.O.^c.n,^C 2 - Haskell Laboratory Report No. 180>85 Material Tested: Sponsor; Polymer Products Department E. I. du Pont de Nemours and Co., Inc. Milmington, Delaware Material Submitted by: )1ymer Products Department E. I. du Pont de Nemours and Wilmington, Delaware Co., Inc. Test Facility; Masked Laboratory for Toxicology Industrial Medicine E. I. du Pont de Nemours and Co., Elkton Road, P. 0. Box 50 Newark, Delaware 19714 and Inc. Study Initiated/Completed; 6/21/84 - 7/6/84 There are 6 pages in this report. Distribution: ^----oesBOcrta'nTSCACBl - 3 - INTRODUCTION B i nis to determine 4 4-hpur Inhalation ALC for male rats. The ALC was defined as the n tested that caused the death of 1 OP more rats either on the day of exposure or within 14 days post exposure, MATERIALS AND METHODS A. Animals Young adult male Cr1;CO*(SD)BR rats were received from Charles River Breeding Laboratories! Kingston, New York. Each rat was assigned a unique 6-digit identification number which was recorded on a card affixed to the cage. Rats were quarantined for one week prior to testing, and were weighed and observed twice during the quarantine period. During the test, rats were in housed pairs in 8" x 14" x 8" suspended, steel-mesh cages in rooms maintained at 25-511 relative humidity and 20-26C on a timer-controlled 12 hour/12 hour light/dark cycle. The rat assigned the lower number in each cage was identified by a slash in the right ear. Rats' tails and cage cards were color-coded with water-insoluble markers so that individual rats could be identified after exposure. Except during exposure, Purina Certified Rodent Chow9 ^5002 and water were available ad libitum. B. Exposure Protocol Groups of 6 rats, 8 weeks old and weighing between 218 and 261 grams, were restrained in perforated, stainless steel cylinders with conical nose pieces. Each group was exoo&fid. nose-only for a single, 4-hour period to a dust atmosphere ofOHfin air. Rats were weighed prior to exposure, and were observed for clinical signs during exposure. Surviving rats were weighed and observed daily for 14 days post exposure, weekends and holidays excluded except when deemed necessary by the rats' condition. C. Test Material Physical Form: Purity: Composition: White to tan powder Synonyms; 'Y^F-?-- ^n^-esl. Does not contain TSCA CB1 Stability: The test material was assumed to be stable throughout the exposure phase of the study. amount of contact of the test material with air was minimized throughout the test. The room D. Atroosphe c Generation U , Dust atmospheres off^Bin air were generated with a 2-stage, vertical glass generaotbr"r./T1rTortund flask served as a dust reservlor. A cyclone-shaped flask. Inserted above the reservior, served as an elutnator. A motorized stirring rod with plastic paddles agitated dust in the generator. Alr^ Introduced at the reservoir blew dust particles upward to the elutriate. A1r Introduced at the elutriator swept dust particles into the expisure chamber. The atmospheric concentration was controlled by varying :he 2 airflows. |^j E. Analytical ---------- The atmospheric concentration of|U||was determined at approximately 30-minule intervals by drawing calibrated volumes of chamber atmosphere through prewelghed glafcs fiber filters. Fitters were weighed on a Cahn model 26 Automatic Electrobalance. The atmospheric concentration of particulate was determined from the filter weight differential before and after sampling. Particle size distributions (mass median diameter and percent respirable) were determined with a Sierra Cascade impactor during each exposure . Chamber temperature was monitored with a mercury thermometer, relative humidity was measured with a Bendix model 566 psychrometer. and chamber oxygen content was monitored with a BioMarine model 225 oxygen analyzer. F. Records Retention All raw data and the final report will be stored in the archives of Haskell Laboratory for Toxicology and Industrial Medicine, Newark, Delaware, or in the DuPont Hall of Records, E. I. du Pont de Memours and Co;. Wilmingto', Delaware. 1 Calculation described in Sierra Instruments, Inc., Bulletin 7-79-219IM, Instruction Manual: Series 21D Ambient Cascade Impactors and Cyclone Preseparators. ;npany Sanitized. Does not contain TSCA CB? RESULTS i A. Exposure Conditions ahd Associated Mortality Dust atmospheres were visible In the chamber during each exposure, frw Chamber temperature ranged between 26-28C, relative humidity ranged 57-601, and chamber oxygen content was maintained at 211. Atmospheric characterization and associated mortality data are presented below, Atmospheric Characterization of TBSA and Associated Rat Mortality Concentration (mg/m ,) Mean S.P. Ranger 820 160 610-1100 2100 1100 1000-4000 % . Respirable" 74 53 , MMD" 5.5 urn 9.5 urn Mortality { deaths/,! exposed) 0/6 0/6 a Percent by weight of particles with aerodynamic diameter less than . 10 urn. Mass median aerodynamic diameter* No additional exposures to higher atmospheric concentrations were tested due to a limited quantity of test material available for testing. B. Clinical Observations During or Immediately following exposure, rats exhibited nasal and ocular discharges, labored breathing, gasping, lethargy, and had test material on their faces and heads. ,Rats were still lethargic 1 hour post exposure. Rats exposed to 820 mg/m had a diminished startle response when the chamber was tapped. During the postexposure period, rats lost approximately 1-81 of initial body eight 1 day after exposure, followed by normal weight gain. No significant adverse clinical 'signs were observed during the 14-day postexposure period. CONCLUSION Under the conditions of this test, the Inhalation greater than 2100 mg/m . This material is considered toxicity by inhalation (ALC greater than 2000 mg/m ). \ ALC for|jH||was to havever^ 'low acute - 6 Company Sanitized. Does not contain T8CA CBI