Document dY5qMZxejB4pkyK1Dwq9ykBO9
TRADE SECRET
AR226-3179
DuPont-6818
Study Title H-24616: Static, Acute, 96-Hour Screening Test to
Pimephales promelas
Laboratory Project ID: DuPont-6818
AUTHOR: Daniel J. Peterson, B.S. STUDY COMPLETED ON: July 11,2001
PERFORMING LABORATORY:
E.I. du Font de Nemours and Company Haskell Laboratory for Health and Environmental Sciences EUcton Road, P.O. Box 50 Newark, Delaware 19714-0050
Company Sanitized. Does not ccmfam TSCA CB1
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CERTIFICATION
We, the undersigned, declare that this report provides an accurate evaluation of data obtained
from this study.
Work Completed by;
-g^a^^^g?. .^Zg^o-^
^usiuel J. Peterson, B.S.
Associate Scientist
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Ifsned by Study Director:
A^^-^ / ^ Robert A. Hoke,PhD.
Senior Research Ecotoxicologist
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Date
Comoany SanKEzed. Dcss not canlam TSCA CBI
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H-24616: Static, Acute, 96-Hour Screening Test to Pimephales promelas
STUDY INFORMATION
Substanc" ^^^Sfiff^
Svnonvms/Codes: H-24616
Haskell Number: 24616
Known Impurities: ^B^BBBj
Physical Characteristics: Opaque tan liquid
Sponsor:
E. L du Font de Nemours and Company Wihnmgton, Delaware 19898
U.S.A.
Study Initiated/Completed: June 25,2001 / July 11,2001 m-Ufe Initiated/Completed: June 25,2001 / June 29,2001
DuPont-6818
Company Sanitized. Does not contain TSCA CBI
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H-24616: Static, Acute, 96-Hour Screening Test to Pimephales promelas
DuPont-6818
SUMMARY
The acute toxidty of H-24616 to the fathead minnow, Pimephales promelas, was determined in
an unaerated, 96-hour, static test.
The study was conducted with 4 concentrations of H-24616 and a dilution water control at a mean temperature of20.7C (range of20.7-20.8C). One test chamber was used per test
concentration with 10 test organisms in each chamber. Based on visual observations, the water control and 1,10, and 100 mg/L test concentrations were clear and colorless at test start. The 1000 mg/L concentration was cloudy at test start. All water quality parameters were within acceptable limits during the exposure.
Exposure of fathead minnows to the water control and nominal H-24616 concentrations of 1,10,100, and 1000 mg/L resulted in 0,0,0,0, and 100% mortality, respectively, at the end of
96 hours. No mortality or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no mortality at test end was 100 mg/L. The lowest nominal concentration causing 100% mortality at test end was 1000 mg/L. Nominal H-24616
concentrations were used for calculation ofLCso values.
The results are summarized as follows:
Nominal concentrations of H-24616, mg/L 96-hour LCso (95% confidence interval)
for H-24616, based on nominal concentrations, mg/L"
dilution water control, 1,10,100, and 1000 316 (100 to 1000)
H-24616 exhibited low concern for aquatic hazard1' in an unaerated, 96-hour, static, acute test using the fathead minnow, Pimephales promelas.
* Stephan, C.E. (1997). Methods for calculating an LC5o. Aquatic Toxicology and Hazard Evaluation, Proceedings of the First Annual Symposium on Aquatic Toxicology, ASTM Special Publication 634 (FJ-. Mayer and J.L. Hamelmk, Eds.), pp 65-84. Philadelphia.
11 Smrehek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation of data. Environmental Toxicology and Risk Assessment, ASTMSTP 1179.
(W.G. Landis, J.S. Hughes, and MA. Lewis, Eds.), pp 22-39. American Society for Testing and Materials,
Philadelphia, PA.
Company Sanitized. Does not contain TSCA CB1
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TABLE 1
DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES
Nominal H-24616
Concentration (mg/L)
Test Initiation (0 hours)
Dissolved
Oxygen
(mg/L)
PH
Test Completion (96 hours)
Dissolved
Oxygen
(mg/L)
PH
Water Control
9.0
7.4
7.7
7.4
1
9.0
7.3
6.9
7.3
10
9.0
7.3
7.2
7.3
100
9.0
7.2
7.3
7.3
1000
8.9
7.2
7.98
7.28
a Measurement taken at 24 hours due to total mortality
TABLE 2 MORTALITY OF Pimephales promelas IN AN UNAERATED, STATIC, ACUTE, 96-HOUR
TESTWITHH-24616
Nominal H-24616 Concentration (mg/L)
Water Control
1 10 100 1000
24 Hours
0/10 0/10 0/10 0/10 10/10
Number Dead /
Number at Study Start
48 Hours
72 Hours
0/10 0/10 0/10 0/10 10/10
0/10 0/10 0/10 0/10 10/10
96 Hours
0/10 0/10 0/10 0/10 10/10
Company Sam-feed. Does not contain TSCA CBI
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