Document dY2VYxavemRgRj3vNdd52pv5R

. AR 226 _ 0920 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: ArefmeirxrteudretocaosntPaiFnOiSng,pFeCr-f9l5u,orooroactsanaescuolmfopnoanteen,twhoficFhXm-3a1y0a4l.so(1b-e Octanesulfonic acid) (CAS # 2795-39-3). Remarks: is FX-3104, 6%T.heC3urMrepnrtodiuncftoiromnatliootnniunmdbiecartewsaistinsoat noted. The test sample mixtureof 0.48% PFOS, 11.44.40%0%sodideituhmyloecnteylglsuylcfoalteb,ut4yl%etihnheirb,it5or9,%2.wa0t%erp,ol1y7o.x6ye%etthhyylelneene glycol, monooctylphenyl sulfate, and 0.1% ether, 1.25% propane benzotriazole. sultone foamer, _ 0.03% sodium lauryl cThhaerafcotlelrowiiznegdscounmcmeantrryataipopnlsieosfitmopaurmiitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical component of the test sample. mETHOO Method: OECD 209 Test type: Static acute GLP: No Year Completed: 1985 Analytical Statistical mmoentihtoodrsi:ng:ResDuilstssolwveerdeodxeytgeernmicnoendcebnytrcaatlicounlsationofthe % Tinehsitbiotirognainn irsesmpisroautriocne:ratAe.ctivated sludge mixed liquor collected from the Metro Waste Treatment Plant, St. Paul, MN. Test condition: Dilution water: Not given. RSyenftehreetniccesaenwdatgees:tspoelrutOiEonCDprmeeptarhaotdion: Not given. Test vessels: Not given. TNeummpbeerartoufrec:oncNeonttrgiavteino.ns: five plus blank control TEolteamlesnutsBpaesnisd:edRessopliirdastiaonndipnhHibiotniondaays of testing: determined Not given. by oxygen `consumption. RESULTS - Nominal mgiL concentrations: 2 Blank controls, 100, 180, 320, 560, and 1000 Element values: 30-minute EC50 = >1000 mg/L 003002 3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. `RSeumbasrtaknsc:e TReesmtairnkgswfaiseldc.onTdhuectveadluoens arempioxrttuerdeaapspldyestcortihbaetdmiinxttuhreeTaensdt not the fiuorochemical proportion alone. CONCLUSIONS Thetest substance 3-hour ECs, determined to be >1000 mg/L. for activated sludge respiration inhibition was Submitter: 3M Paul, Minnesota, Company, 55133 Environmental Laboratory, P.O. Box 33331, St. p0 ar0 agu0 ay0000000000 Rmeeltihaboidliftoyl:lowKeidi.misTchherainnkoicnuglu3.m wSatsudnyotlapcrkospedreslcyrcihpatriaocntaernidzerde.coTrhdseorfetihseno cchoanrfaicrtmeartiizoantoifontoheftahmeotuensttosfufblsutoarnoccehepmuriictay,l apnrodpotrhteisotnuidnytlhaecskoslautniaolny.tical REFERENCES TSth.isPasutlu,dMyNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMC1C1o1m1p-a2n,y1,98E5n.vironmental Laboratory, OTHER Last changed: 6/26/00 00300: - ~n A ENVRONMENTAL _ hABCRATORY T= MicroBiAL Toxemy AcTvATED S1uDEE RESPIRATION hwismon Tesr-CECD 209 TestSueswce i SUEY470 LitUotr Test Seam: SumoF AnuatedSkudee Mixed hu we BEST COPYAVAILABYETMRecenev2/20E/x DaveUsed. 2L2a/k= 0 MINUTE INSSoLNED (OXYGEN TR eADINGS [re rlcr]SS usE sT sComgEmNTrRAmTNeSns i| [L0 122X2 ou 0.9 P QF o0.GfdeO dFos] QS | 2& .7] 2[1p[resfsl [7 TT TT 91] =[oy[ TTTTT5 | l(a=T1ep~e ] T |T TT1 T 1 [&%74 Le[TTTr F(e= 1 Ir -- Tr r 1 r 1 1 L Fo a|T T 1T T T 1 T 1 --1 T-- --] 1 hau TDissouved Oxveaen FReADIGs . Tegr Sussmge Co ATO at F la1s|2 =92|F2a%zlTa=d T=ozlTTaegaT |4 4a981T=2 ) LaToe [1s[20 va [a7 |ad[2g | LTTio [7sTd[az 20 | 6 | Le T= TT Tx 27 r|! 1T 1 T 17s[za I laI T1791ozI 1 . 007004 A ENVIRONMENTAL, LABORATORY Deas . Actuated Siunee Micrpesns RespreaTion Toxony Miso INHTI estB CaI reuT smoI ns O OECN D! "% Iumamon En 100 R= OceenConsutermonFate. (waOa/L aud win) S =Test Sunsmxe C,=CasrmoTn Cz= Controw IT BEST COPY AVAILAB O vine Coup IT [00wslf 212800 aglllf Stow 1000wall CowontL "[DiOssogneEed tOvleonsrCA%LmCULoAwTIwtONsimonde]| "Ha HER NSB 30 Mure.ECasttmroncctme) 200ml [pom Pmeamare Hox DD oLveED (Owe ALCIY [a 100ual) 180af 220wall Soo wall 1000 wqlf Coomera MC 0329 Lo bzbz - bol Lots Rr 421 + 000 ATS -- o 14 1s 22 =) -- 3BHOUR, ECs, (35% Corrmace LuMis) 200 J . Accermairye of Test (In Conrros': % Dirvereace "BER Zz *PeRCENT DiverNET Somme Cormas =AS EXER Fh, S--ois