Document dQeMB3q56oQ1Z1Gp1Zrdvp3dR

BACK TO MAIN ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE_____________________ Identity: Decafluoro(pentafluoroethyl)cyclohexanesulfonate, DEA salt; may be referred to as CS-2070. When dissolved in water may also be referred to or as CS-2151 or FC-90. (DEA salt of perfluoro(ethylcyclohexyl) sulfonate, [C2F5-CF(CF2CF2)2CFSO3-][H2N(CH2CH2OH)2+]) Remarks: The 3M production lot number was 3. The test sample is CS-2151, a mixture of CS-2070 in water (approximately 25% test substance and 75% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. The sample purity was not characterized. METHOD: Method: Standard Practice for Conducting Acute Toxicity Tests with Fishes, Macroinvertebrates, and Amphibians (ASTM E729-80) Type: Acute static GLP: No Year completed: 1980 Species: Pimephales promelas Supplier: Dale Fattig Fish Farm, Brady, NE. Analytical monitoring: Temperature, pH, and dissolved oxygen. Exposure period: 96-hours Statistical methods: LC50 values calculated using the probit program and 3M TRAC Computer System. Test fish age: Juveniles Length and weight: Average length = 35 mm Average weight = 0.3 g (wet) Loading: 0.4 g/L Pretreatment: None Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry: provided for the end of 3 rd qtr, 1980. Uclear when the toxicity study was conducted. Hardness: 244 mg/L as CaCO3 Alkalinity: 230 mg/L as pH 7.7 Conductivity: 380 mmhos/cm Dilution water chemistry ranges during the study: Temperature: 19 + 2oC pH 7.6 - 8.0 Dissolved oxygen at 96-hours: 5.2 - 5.9 mg/L Lighting: A daily photoperiod of 16 hours light and 8 hours dark with a 30 minute transition period was maintained throughout the testing period. Stock and test solution preparation: Not noted. Concentrations dosing rate: Once. Stability of the test chemical solutions: Not noted. Exposure vessels: All-glass aquaria (35x20x25 cm) containing 16 L of test solution. Number of replicates: 2. Number of fish per replicate: 20. Number of concentrations: five plus a blank control RESULTS________________________________________________________ Nominal concentrations: Blank control, 87, 135, 210, 320, and 490 mg/L Element values with 95% confidence interval: 96-hour LC50 = 175 (156-195) mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the test substance as described in the test substance remarks field. The values reported are based on nominal concentrations of CS-2151. CONCLUSIONS________________________________________________ The test sample 96-hour LC50 for fathead minnow was determined to be 175 mg/L with a 95% confidence interval of 156 to 195 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY________________________________________________ Reliability: Klimisch ranking = 3. The results are from a summary report and there is no concentration-response data included. There is a lack of characterization of the test sample. Test solutions were not analyzed for test substance concentrations and confirmation of the amount of fluorochemical components in the solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 6260, 1980. OTHER_______________________________________________ Last changed: 4/01/01