Document dQNbMmGJajrmezEGm8Vk9N5bb

ENVIRONMENTAL HEALTH REPORT February 3, 1978 PERSONNEL BLOOD SAMPLES INTRODUCTION Blood samples collected from two employees at the Solvents Plant and from a plant visitor (Carl Kesler) were analyzed for chlorinated hydrocarbons. The chemicals were extracted from the blood with nano grade hexane using an ultrasonic technique. Analysis of the hexane extract was performed by electron capture gas chromatography. The chemicals analyzed include vinylidene chloride (V2), chloroform (CHCI3), carbon tetrachloride (CCl^), 1,1,2-trichloroethane (B-tri), perchloroethylene (Per), trans 1,2,3-trichloropropene (trans TCP), cis 1,2,3trichloropropene (cis TCP), hexachloroethane (HCE), hexachlorobutadiene (HCBd), and hexachlorobenzene (HCBZ). A linearity study of the analytical method was also conducted and found to be within acceptable limits. EXPERIMENTAL Standard Preparation: A stock solution of the 10 compounds was prepared by injecting 30 microliters of each organic into a 16 X 150 mm culture tube containing 10 ml. of nanograde hexane. Thirty (30) microliters of this stock was then transferred to another culture tube containing 10 ml. of hexane to give a second stock which contained between 10 - 14 ug (depending on the density) of chlorinated hydrocarbon per milliliter of solution. Working standards of 25, 75, and 150 mg. per ml. were then prepared by injecting sufficient quantities of the second stock into three culture tubes con taining 10 ml. of hexane each. These standards were analyzed to check the linearity of the detector. DO 08B9P4 CONFTDENTIAI February 3, 1978 PERSONNEL BLOOD SAMPLES Page - 2 - Analytical Parameters: A Hewlett-Packard, Model 5700 A, gas chromatograph equipped with a Ni63 electron capture detector was used for the analyses. Chromatograph conditions follow: Column: 10 X glass column, 15% Dexsil 300 on 80/100 MESH 50/50 Gas Chrom Z - Gas Chrom W-(AW) DMCS treated Temperature: Oven, run #1 Oven, run # 2 Oven, run # 3 80 C 140 C 250 C Injector Detector 200 C 250 C Carrier Gas: 5% CH4/95% Ar at 40 cc/minute Sample Size: 7 microliters Elution Times: 250 C Hexachloroethane Hexachlorobutadiene Hexachlorobenz ene 2.0 minutes 2.4 minutes 10.8 minutes 140 C 1,1,2-trichloroethane Perch loroethylene Trans 1,2,3-trichloropropene Cis 1,2,3-trichloropropene 2.9 minutes 3.3 minutes 4.3 minutes 5.4 minutes 80 C Vinylidene Chloride Chloroform Carbon tetrachloride 2,3 minutes 3.6 minutes 4.6 minutes PROCEDURE Sample Collection: Each blood sample was drawn into a 5 ml. green top heparinized tube in order to prevent clotting, All samples were then frozen until analyses could be performed. 088975 CONFTDFNT Ja| February 3, 1978 PERSONNEL BLOCS) SAMPLES Page - 3 - Sample Preparation: After the blood samples had thawed to room temperature, four milliliters of each sample were pipetted into 16 X 150 mm culture tubes containing 5 ml. of 2% Na2S04, and agitated for 20 minutes on a wrist action shaker. Four (4) ml. of hexane were then pipetted into each culture tube and the resulting mixture agitated again for 20 minutes. The culture tube was then placed into an ultra sonic bath for 60 minutes, agitated for 20 minutes, and finally centrifuged for 45 minutes. The hexane layer was pipetted from the culture tube and trans ferred to another culture tube containing 2-3 grams of anhydrous Na2S04 (drying agent). Analysis of the hexane extract was performed by electron capture detection gas chromatography and the peak height of the resulting trace measured. Calculations: Nanograms per ml. of hexane extract of each chlorinated hydrocarbon was calculated from the chromatogram with the following equation: ng/ml (chromatogram) = (attenuation)(standard response factor) The parts per billion by weight of chlorinated hydrocarbon in whole blood was then determined as follows: ppb(w) = (A - B) 4 C where: A = nanograms of chlorinated hydrocarbon per ml. of hexane extract obtained from the whole blood sample. B = nanograms of chlorinated hydrocarbon per ml. of hexane extract obtained from the blank. C = grams of whole blood DO 088976 CONFTDFNTTAL February 3, 1978 PERSONNEL BLOC SAMPLES Page - 4 - RESULTS AND DISCUSSION The results of blood samples are shown in Table I. The concentrations of all chemicals detected are expressed in parts per billion by weight. Hexachlorobenzene was the predominent containant in all samples. L. R. Smith Indistrual Hygienist Chlorinated Solvents ^0 088977 confidfnttai TABLE I PERSONNEL BLOOD SAMPLES " ppb (w) NAMF. OF RMPT.OYEE Cy Bacrios Art Bourg Art Bourg Cy Barrios Art Bourg Art Bourg Carl Kesler DATE SAMPLED 9/22/77 9/22/77 9/27/77 10/3/77 10/5/77 10/7/77 1/27/78 TIME 2 PM 2 PM 9:30 A 8 AM 7 AM 9:30 A 2 PM V2 N/D N/D N/D N/D N/D N/D N/D CHCI3 12.7 16.0 22.6 17.1 18.7 21.6 28.2 CCI4 7.4 9.2 14.5 4.3 9.9 12.9 22.3 B-TRI 7.3 6.9 17.3 18.0 3.3 5.3 21.3 PER 13.4 16.0 7.7 12.7 7.3 23.6 27.0 TRANS TCP 0.8 1.1 0.7 1.5 0.4 2.7 4.3 CIS TCP 0.6 0.9 0.6 1.5 0.7 1.1 4.0 HCE 7.0 12.1 5.1 5.3 9.7 12.0 10.8 HCBD 3.0 4.1 2.5 3.0 2.7 4.3 6.0 <c 0C t-1 C\ H OZ OC Uj CC C c Ll cz cC HCB2 197 713 643 185 654 810 123 N/D = Not Detected DOW COMFlOuNTlftL