Document dQ9kbr28ovgq7g09X3KbGBdoq

fROCRESS REPORT ORGANIC CHEMICALS (VISION nCHNOLOCY OtPAKTmtfT " W3<iHO*e W. M. Mess W. J. Litschgi J. D. Hinchen T.. S. Tucker ron March August, 1971 408 el or r a it. a No r to 0*"C 1 8-31-71 St. Louis - I. Second St. 2-21-760.01-13480, 85010, 85050 TMi I Kltrwtln, * If msmnv * IMW3AKT COWANY. ii COWKOSNTIAL INFORMATION --MCM "Mt w ta <w *>< *iw*m tf Osm*r*t mt&Hmt mm* mfrmimtum. TN# mnmmt iee*8*( t ita OiSTtllUTlOH *ith sc tails roi RESTRICTED TOi H. S. Bergen T. M. Patrick 3e IjCchtiililTIAi D e Hi-HChCn ** = Keller *R. H. Munch *W. B. Papageorge *C. W. Root *w. R. Richard s. P. Wheeler/G. Levinekas E . S. Tuckar/W. M. Meea/ H. J. Litschgi *<*.8 ENVIRONMENTAL ANALYTICAL PROGRAM - TOXICOLOGY SUPPORT STUDIES t * kvl C * t Determination of the type and level of polychlorinated biphenyls (PC3's) retained in the tieeuee of laboratory animals fed Aroclor 1242, Arcelor 1254, and Aroclor 1260 for correlation with toxicological effects. SUMMARY . Analysis of 160 tissue samples (liver, fat, kidney and muscle) excised from albino rate sacrificed at 3, 12, and 24 month intervals during a two year study (1ST - 17298) of the chronic oral toxicity of Aroclor 1242, Aroclor 1254 , and Aroclor 1260 have been completed. The residues retained in the tissues of the animals fed these products wore isolated by solvent extraction and the level and type of PCS'* determined by electron capture gas chromatography. PCS residues from these Aroclor products wore observed in all the tissues (liver, fat, kidney and muscle, at all levels fed (1, 10 and 100 ppm) and at each sacrifice interval (3, 12, and 24 months). The residue levels found in the tissues of the smimal fed 1, 10 and 100 ppm of Aroclor 1254 and 10 and 100 ppm of Aroclor 1260 continued to build up over the entire two year duration of the study. Storage levels in the tissues of those animals fed 1, 10, and 100 ppm of Aroclor 1242 and 1 ppm of Aroclor 1260 did not continuously increase, but instead began to plateau at some point after three months of exposure. The average PCB lipid storage level found in the tissues for each product after two years of oral exposure are shown in the following table. kqns 0 5U^ HARTOLDMONO015582 2-21-760.01-13480, 85010, 85050 Kerch-August, 1971 Page two fC* Storage Level (k?A(r) .Level fad 1 ppm 10 ppm Aroclor 1242 11 Aroclor 1254 58 Aroclor 1260 54 58 348 411 100 ppm 233 1989 5508 Alteration of the hooolog diitribution of all products was also observed. The alteration was greatest for Aroclor 1242 and least for Axoclor 1254 and Aroclor 1260. The dominant PCS homologs found in the residues were in all cases those which have been observed as environmental residues. Although the observation of gross toxicity effect caused by the in gestion of the PCS products were few, the data indicated that they occurred (enlarged livers) when the PCS lipid storage levels were in the range of 500-1000 ppm. FUTURE FLAKS Analysis of selected tissue samples for the level and type of PCS residues retained from the following feeding studies carried out by Industrial Bio-Tast Laboratories. Study IBT IC7299 "Two Year Chronic Oral Toxicity Study of Aroclor in Beagle Dogs' (180 Samples) Priority 1 IBT IP7297 'Three Generation Reproduction Study in Albino Rats with Aroclor' (90 Samples) . 2 IBT IJ7300 "Toxicity, Reproduction, and Residue Study of Aroclor in White Leghorn Chickens * (210 samples) 3 db 9/2/71 W. H. Hees ~. 3" /ucJLc*-- Z. S. Tucker HONS 057147 HARTOLDMONO015583 2-21-760.01-13480, 18010, 1S050 March-August, 3J71 Page three DETAILS See the following referenced report* t Two Year Chronic Oral Toxicity Study of Aroclor in Albino Rats', Industrial Bio-Tet Laboratories, Report IBT 17298. "Determination of Polychlorinated Biphenyl Residues in Rats From A Two Year Chronic Oral Toxicity Study, Analytical Chemistry Special Study 71-7. Statistical Analysis of Bio-Ta*t Oral Toxicity Study - Albino Rats*, Statistics Special Study 71-14. HQhS 0S?lU HARTOLDMON0015584