Document dQ78GXjN7j34041EjbBJQxMrq
AfOT'050$
CHRONIC TOXICITY TO FRESHWATER INVERTABRATES (Daphnia magna)
TEST SUBSTANCE
Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, FC116, FC-126, FC-169, orFC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # 3825-26-1 )
Remarks: The 3M production lot number is 264. The test sample is FC143. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 96.5 - 100% test substance and 0 3.5% C6, C7, and C9 perfluoro analogue compounds. The 3M production lot number is 264.
METHOD:
Method: USEPA-1982, OECD 1981. Type: Semi-Static Life-Cycle Toxicity GLP: No Year completed: 1984 Species: Daphnia magna Supplier: In-house cultures, 3M Environmental Lab, St Paul, MN Analytical monitoring: Temperature, pH and dissolved oxygen Exposure period: 21 days Statistical methods: Probit analysis (for acute toxicity values) and Moving Average Angle Method for chronic study. Statistics were recalculated by AScI Corp., Duluth, MN in 1998. NOECs and LOECs were generated using Toxstat. Reproduction was normal and
homogenous therefore Dunnett's Test was used. Fisher's Test was used for survival. IC50S (reproduction) were generated using ICp program. Survival EC50S were generated using Trimmed spearman-Karber. Test organism age: < 24-hour old neonates for test initiation. Pretreatment: None
Test conditions:
Dilution water: Aerated carbon-filtered well water
Dilution water chemistry (sampled one month prior to study
initiation)
hardness: 240 mg/L as C aC 03
alkalinity: 230 mg/L as C aC 03
pH: 7.8
Conductivity: 340 pmhos/cm
COD:
< 0 .4 mg/L
Stock and test solution preparation: Information not available
003824
Renewal rate: Every two days Exposure vessels: 250-mL glass beakers containing approximately 200 mL test solution. The depth was approximately 5 cm. Beakers were covered with watch glasses Number of replicates: 4 Number of test organisms per replicate: 5 Loading: 1 daphnid per 40 mL Number of concentrations: 6 plus a blank control
Feeding: A suspension of fish food (trout chow) and yeast containing 5 mg dry solids per one mL of mixture was fed on a daily basis Lighting: Cool-white fluorescent lights. Intensity ranged 50-100 ftc. Photoperiod of 16-hours light, 8-hours dark with a 20-minute transition period^
Water chemistry of new and old solutions during the study: PH and dissolved oxygen: Information not available Test temperature range ( 0 - 2 1 days): 2 2 + 2C
Element basis: Survival and reproduction.
RESULTS
Nominal concentrations: Blank control, 5, 8, 13, 22, 36, and 60 mg/L Element value: 14-day NOEC (survival) = 60 mg/L
14-day NOEC (reproduction) = 8 mg/L 21 -day NOEC (survival) = 22 mg/L 21-day NOEC (reproduction) = 22 mg/L 14-day IC50 (reproduction) = 40 (28-48) mg/L 21-day IC50 (reproduction) = 43 (35 - 46) mg/L
All element values based on mean measured concentrations
Biological observations Survival: All surviving first generation daphnids appeared normal at test termination. Survival in the 36 and 60 mg/L treatments was statistically significantly different from the negative control group. Reproduction: Daphnids in the control started producing neonates on Day 7. Reproduction was statistically significantly different from the control (Dunnett's) at the 13, 22, 36, and 60 mg/L concentrations after 14-days, and in the 36 and 60 mg/L test solutions at 21-days.
003825
Summary of Percent Mortality
Nominal
Cone., mg/L Day 14
Day 21
Negative Control
0
0
5.0 0 5
8.0 0 20
13 0 20
22 0 20
36 0 25
60 5 --80
Reproduction after 21-days
Nominal Number of Mean Live
Concentration, Surviving Young / Adult
mg/L
Daphnids Daphnid (+ SD)
Negative Control 20
165 + 13
5.0 19 145 + 29
8.0 16 138 + 52
13 16 131 + 38
22 16 130 + 11
36 15 108 + 24
60 4 36+12
CONCLUSIONS
There were no adverse effects on survival or reproduction Daphnia magna exposed to the test substance at concentration <22 mg/L for 21 days.
Author and/or submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Klimisch ranking 3. Study was apparently well conducted according to the methodology available at the time of test initiation.
003826
Information was not available on test substance purity. The raw data for this study could not be located, and some key study elements could not be evaluated at this time. REFERENCES This study was conducted at the 3M Company Environmental Laboratory, St Paul, MN. Recalculation of the effect and no-effect concentrations was performed at AScI Corporation, Duluth, MN Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 OTHER Last changed: 5/25/00
003827
t
ENVIRONMENTAL LABORATORY
l* C 2 * S Z 3
DATA SUMMARY REPORT ON THE'TESTS FOR
ACUTE & CHRONIC TOXICITY OF FLUOROCHEMICALS
T0 PAPHNIA MAGNA (WATER FLEA)
(9972512600)
TEST SUBSTANCE
Descriptions FC-lM-S WjoT Z J p 4 Additional Identifications
STATIC ACUTE TEST
Begins 8 / 2 . / B 4- Ends 6 / 4 / 8 J,|- D urations 1 4 0 HOLTZS Dose Range sZ S , *40, [Sy \0 D ; )L0; 2JSO, 4 0 0 ,( ^ 3 0 Temperatures ZJLC^
Resultss
- 24-Hr. EC5q (95% Confidence Limits) s 41 ip - 48-Hr. EC50 (95% C.L.)s ZUk> (31-
RENEWAL CHRONIC TEST
Begins 8 / 7 / 8 4 Ends ^ j 3 / 8 4 Durations Z1
Dose Ranges 5)8, 13,2J2L} 3 k , U>0 Temperatures ZZ,-- \CL
Resultss
Young Reproduction
Impairment
Approximate NOEC
l*>
MATC (Limits)67M-M#rr(L i*i Cis>-zjO
1 4 -Day EC50 (95% C.L.) 3 5 (2i.->45)
TLX -Day EC5Q (95% C.L.) 3 8 C s s ~
Chronic/Acuta Ratios
Application Factors (MATC/Acute EC50)
Env. Safety Factor
(MATC/EEC)
AdUlt8
Mortality
i m a UI
Ztt
>yo
*hcJJI
40(3\-5 Z )
003828
* A &4-&4iM4CaI S tyifftcdstfiC . "bs-Sf U3ae>^ (b rrv te d l U4**tc\ 'P*OOS-
2
ACCEPTABILITY OF CHRONIC TEST
The P maqna control population have met the following quality criteria:
- Adults Mortality:
ArOArE (max. 20%)
- First Brood Release:
y2WC5 (max. 9 days)
- Cumulative No. Young per Adult (14-Day)iVZlmin. 20)
- Cumulative No. Young per Adult (21-Day) tJ^Snin. 40-60)
- EphippiS:
A 2?/i/(none)
- D.O. Saturation: ~
6 % ( m l n . 60%)
- pH Level:
8.0t02lm a x * * 5 from initial)
COMMENTS
Reported and Approved by:
N.
Date: z / y / e /
003829
TABLE I. SUMMARY OF ACUTE TOXICITY OF PC - ( 4 5 VCTr 2 ^4-________
/ TO DAPHNIA MAGNA UNDER STATIC EXPOSURE CONDITIONS(1 *
------------------------------ ------ -------
CHEMICAL TREATMENT
(mg/1)
24-HR. EXPOSURE
MORTALITY
(%)
.
48--HR. EXPOSURE
MORTALITY
(%)
CONTROL
o
o
40
ie3
lo o
\u>o ZJ50
Hoo
0
Oo
o o
o o
o o
o
15
s 50
4o q o
<\5 l o o
ECc0 (95% CONFIDENCE LIMITS)(23
mg/1
'
4l(i> ( s
H-T2-)
Zkle (31--50*?-)
i
(1)
(2) n e to
Data are averages of two replications for. each treatment with ten (10) daphnids per replicate. A total of twenty daphnids were used per treatment.
EC5o values were calculated using logistic regression methods (Probit Analysis).
ENVIRONMENTAL LABORATORY TABLE II. SUMMARY OF CHRONIC TOXICITY OF t T M 4 3 > }U T T ? Ip4
TO DAPHNIA MAGNA UNDER RENEWED STATIC EXPOSURE CONDITIONS*1*
CHEMICAL TREATMENT (mg/1)
14-DAY EXPOSURE
YOUNG REPRODUCTION ADULT
IMPAIRMENT (ft)
MORTALITY (ft)
21-DAY EXPOSURE
YOUNG REPRODUCTION ADULT
IMPAIRMENT (ft)
MORTALITY (ft;
CONTROL 5
8 13
ZJL
3U>
Ip0
OO
o
14" O
\7L>
Zip
O'
Up
3P O
Z\
36 O
2J
41 O
35
t>8 5
18
EC-0 (95% CONFIDENCE LIMITS)*23 3 , 0
mg/1
3 5 ( z J e - 4 5 y s>
>1*0
HjqkisttesVconcent
40 38
O
5
zo 2X>
ZS>
ZS 80
4Z-C3+-SU)
M o (31 - S O e:
Data are averages of four replications for each treatment with five (5) daphnids per replicate Q total of twenty daphnids were used per treatment. C4
EC50 values wer calculated using logistic regression methods (Probit Analysis).
A
values
calculated usm^ Moving A*ierocp-
Hc4Vvod.
You wft_'r^w^oEucnowi
( V nuumoe. kWoeep*
TEST ^UBSTAWCfe-J & r J ^ S M T sP J ^ --- TESrborzJZ/Y/frV--%/&/,
S a ^ lo
W
3^^^'Pr^ kC>'^ fe<r""Tt+ llv-h ...
32.Z.
LDWTTpO ^ s
34-4
' 340 348
1 344 * 51___
I 2JoO
5 urn A ^
--^ X
8 Ktia/J!
y --S T?=
353 Z3S -S^T 1311 H~|) 0.1+50
181 308 >o4 3qi
.bS \o 0.1^08
0KApt+5H\
2J 3 )^
-A^ulR- ^ ? r ~Ts+.lAy4'. ~-^rrAfki 1+
04.4
455
150-0
y \A
16 4S.l>
'IZ J * k>
84o 4o8 802^
1lo
181.(o l (o0.4 ltoS"^=l3
52
32.8
105.(0
To.tc
8(37
145.4
34 , (<54.+ (>23=4 0 .143
4 (8 444
Y23=t|43 0 12 fc03 .
143-(o 155.4
I`4 5 -1
Oj ZMS>5
3 1 .4
Lp l io 3 3 -4 14.4-
334
142 . 340
4 lS
l 2>,lS; 143.(00
14
185.55
3523 0ii4OQ
te483 2-4 3 138*32
0 5 i4
0.3041
IS I
l w jp l
i^
X S =
384-
2 Z Ji 111 ` 1 0. lOo
<30,_ H1a (^ ^sue.1b,6
4(o 4=22 0.1080
4ofc. (o4M 55(o
42..(0 134.25 11 2 8 5
T i 1822. >03832
3|2_12 131 3 8
0 114(>0
o .\&lA-
Yo u Mv'T^erfVucnoiJi^
(^uM.ubrtnoe. M u m KP)
-resr auBswwce_^2LS-- A TtC rr--- test-
La2^t23
j/y/frv- froe /ft
W 3teS C rto tio A ^ T^(r*Tc<r+- l JUG-h
'AA V+-
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^ f i T l v-k
oAMvi 1+
G smT ^ O U
^ s _____ I
2 .4 1 2 -2 .8
ZSLmlX ^
1 5lp
X f^JS
Z^ZH H g
o .o o s ^ l*U L /lH >5
3? ^ . S T 5=
164 \9 i^
o . o o i 'S
4 9 .4
HS.>
5 3 .2 3 1 .2 4 10
O .O O S iO *2 J B .4 M - 8 i (p
3b,e
see
0 .0 0 1 3 ^
5 to 4 Ip 5 1 1o4 su to 2 to to i
0,00'1P> *
334
SQlp
343
5 2 3 dr I3 0 O .O I4 -I ^
121.S 1 3 5 -4 143 II1 .5 S (3 0 H
0 ,0 0 9 2 *1 4 0 5 n q .2 1 1 0 .4 I2 J .5
I0 8 2 H 0 ,O I3 \*
x " - S> C =
1 IO S
lo i
lis 73 ' 1 0 .0 0 o c * '
-i SI 2 .0
2 0 .2 .
23S 0 ,0 0 0 1 ^
IIS 2o2_I5 0
2 4 ,3 5 3 2 .4 3 1 ,3"
154 155 i3 t o o .o o o r*' '
3 4 .2 -1 C 03S 33 c 5 t o -- 12. O .o o n n *
MTE/RRR
ENVIRONMENTAL LABORATORY
1-3-84
SUMMARY OF EXPERIMENTAL DESIGN AND CONDITIONS FOR CONDUCTING
RENEWAL CHRONIC EFFECTS TESTS WITH DAPHNIDS
Project: Fate of Fluorochemicals - Phase II (9972512600)
Exposure: Type - renewal static Renewal frequency - every 2 days Duration - 14-21 days
Test Species: Daphnia magna or Paphnia pulex (use of D. magna is preferred) - Age of test animals - 12+12 hrs instars (neonates) - No. animals/beaker - 5 - No. of replicate test beakers per concentration - 4 - Total No. animals per concentration - 20
Test Vessel: 250 ml glass beaker (Pyrex) covered with a watch glass
Test Solution Volume: 200 ml
Loading Ratio: One animal per 40 ml
Dilution Water Supply: Aerated carbon-filtered well water (Bldg. 2) of consistent chemical quality. Water hardness classification: hard (hardness ^ 250 mg/L as CaCO^).
Untreated Controls: 100% well water
Toxicant Dose Range: Minimum of five logarithmic concentrations (by weight)
Test Conditions:
Temperature - 22j*2C (68-75F)
Light Quality - Type - ambient laboratory light
(cool-white fluorescent) .
- Intensity - ambient lab levels (50-100 fc)
- Photoperiod - 16 hrs light; 20 minutes
transition period
Aeration During Test - None
000834
Food and Feeding Regime: A suspension of fish food (trout chow) and yeast (Fleischmann's) containing 5 mg dry solids per one ml of mixture, on a daily basis.
Measurements and Observations: Effects (noted daily and recorded on renewal days) - No. young, live and dead; brood size, and no. broods - No. dead adults - Immobility, presence of unhatched eggs or ephippia, and other signs of stress Water Quality (of dilution water and of replicates of old test solutions made on renewal days) - Physical: temperature, D.O., and pH - Chemical: analytical measurements of toxicant concentrations in initial stock and in old test solutions (optional)
Validity of Test: * The control population of D. maqna should meet the following requirements: - Mortality of adults - maximum 20% - First brood release - maximum 9 days - No. young per adult (14 days) - minimum 20 - No. young per adult after 21 days - minimum 60 - Ephippia - none - D.O. saturation - minimum 60% throughout the test - pH - maximum +0.5 units from initial
Treatments of Results: 48-Hr Acute Test - 24-hr ECgg - 48-hr ECgg with 95% confidence limits 14-21 Day Chronic Test&^ - Approximate no observed effects concentration "NOEC" - 14- and/or 21-day EC5Q with 95% confidence limits - Maximum acceptable toxicant concentration "MATC" - Chronic/acute ratios - Dose/response curve based on cumulative mortality of adults - Safety factor
003835
References : - USEPA-1982. Env. Effects Test Guidelines. EPA560/6-82-002 - OECD-1981. Guidelines for Testing of Chemicals - ASTM-(latest drafts, 1983). Proposed Standard Practice for Conducting Renewal Chronic Effects Tests with Daphnids
*Final results are calculated from mean values of cumulative no. young per adult, or cumulative mortality of adults.
Definitions ;
Daphnia Chronic Toxicity Test - A 14-21 day experimental study of the survival, growth and reproduction of asexually reproducing Daphnia beginning with- 12+12 hr old organism.
Significant - "Statistically significantly different" refers to p<0.05 with respect to the control.
Approximate NOEC - The highest test concentration that will produce no significant observed effect on reproduction of young or adult survival.
* Chronic EC5Q(s) - The values of toxicant concentrations which impair reproduction of young or induce adult mortality by 50%.
MATC - The highest concentration of toxicant that has no adverse effect on survival, growth, and reproduction of a species.
GM-MATC is the calculated geometric mean between the highest test concentration having no significant effect and the lowest test concentration having a significant effect.
Application Factor (AF) - The quotient of the chronic MATC divided by the acute 48-hr EC5q (MATC/Acute EC^g). "AF" is a number used to estimate concentration of toxicant that will not cause significant effect to a population during chronic exposure.
Environmental Safety Factor - The ratio of the chronic MATC with the expected environmental concentration (MATC/EECf. Safety factors >20 at the predicted exposure level are desirable to have sufficient confidence in the environmental safety assessment.
003836
8-23-84
ENVIRONMENTAL LABORATORY PINAL REPORT
LAB REQUEST NO. B2055
REQUESTER NAME: RRR DEPT: 0222 PROJECT NO: 06 DATE RECEIVED: 8-16-84 DESCS JUNE CARBON-FILTERED NELL RATER
CONTRACT LAB:
CONTRACT LAB COST:
0
3M E-L HOURS:
3
EST CMPLT DATE: 8-24-84
DATE COMPLETED: 8-24-84
SAMPLE
DATE
CODE
DESCRIPTION
TEST
RESULT
1
7-06-84
RM #4
2 DI HATER
PH RES-CL COND TOT-HARD TOT-ALK TS NH3-N SOL-F COD-LOW TQT-COLIF SPC
PB RES-CL COND SPC
7.8 <0.02 MG/L 340 UMBOS/CM 240 MG/L 230 MG/L 270 MG/L <0.4 MG/L 0.2 MG/L <0.4 MG/L NEGATIVE 54 PER ML
6.6 \ <0.02 MG/L 1.8 UMHOS/CM 14 PER ML
* * CONTRACT LAB APPROVED AND SUBMITTED BY
dated
y
I
G 03837
August 10, 1998
4444 Airpark Boulevard, Duluth, Minnesota 55811
1218) 722-4040
Fax (218) 722-2592
Ms. Susan Beach 3M Environmental Technology and Safety Services 935 Bush Avenue Building 2-3E-09 PO Box 33331 St. Paul, MN 55133-3331
Dear Sue:
FC-143 Daphnia magna reproduction data for concentrations above the survival NOEC were not included during hypothesis testing for the reproduction NOEC and LOEC. EPA guidance stipulates that concentrations which significantly effect survival should not be included in hypothesis testing for reproduction. The exclusion of such concentrations usually results in a more powerful and appropriate statistical analysis.
Joe Dierkes Biologist, AScI
003838
Title: FC-143 DAPHNIA MAGNA 21 DAY REPRODUCTION
File:
fc21d
Transform:
ANOVA Table
NO TRANSFORMATION
SOURCE Between
DF SS
MS F
4
115876.3000
28969.0750
1.0871
Within (Error)
15
399732.5000
26648.8333
Total
19 515608.8000
(p-value = 0.3980)
Critical F = 4 . 8 9 3 2 (alpha = 0.01, df = 4,15) = 3.0556 (alpha = 0.05, df = 4,15)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)
Press any key to continue or ESC to return to m e n u . ..
Title: FC-143 DAPHNIA MAGNA 21 DAY REPRODUCTION
File:
fc21d
Transform:
NO TRANSFORMATION
Dunnett's Test - TABLE 1 OF 2
H o :Control Treatment
GROUP
IDENTIFICATION
TRANSFORMED MEAN
MEAN CALCULATED IN
ORIGINAL UNITS
T STAT
1
CONTROL
825.7500
825.7500
2
5 MG/L
722.5000
722.5000
0.8945
3
8 MG/L
647.7500
647.7500
1.5420
4
13 MG/L
630.5000
630.5000
.1.6915
5
22 MG/L
625.5000
625.5000
1.7348
Dunnett critical value = 2. 3600 (1 Tailed, alpha = 0.05, df = 4,15)
Press any key to continue...
Title: FC-143 DAPHNIA MAGNA 21 DAY REPRODUCTION
File:
fc21d
Transform:
NO TRANSFORMATION
Dunnett's Test - TABLE 2 OF 2
Ho:Control<Treatment
GROUP
IDENTIFICATION
NUM OF REPS
MIN SIG DIFF
% OF
DIFFERENCE
(IN ORIG. UNITS) CONTROL FROM CONTROL
1
CONTROL
4
2
5 MG/L'
4
272.4182
33.0
103.2500
3
8 MG/L
4
272.4182
33.0
178.0000
4
13 MG/L
4
272.4182
33.0
195.2500
5
22 MG/L
4
272.4182
33.0
200.2500
Press any key to continue or ESC to return to m e n u . .. 003840
Title: FC-143 DAPHNIA MAGNA 21 DAY REPRODUCTION
File:
fc21d
Transform:
Shapiro - Wilk's Test for Normality
D = 399732.5000 W = 0.9767
Critical W = 0.8680 (alpha = 0.01 , N = 20) W = 0.9050 (alpha = 0 . 0 5 , N = 20)
NO TRANSFORMATION
Data PASS normality test (alpha = 0.01). Continue analysis.
M
iir/>ro
AJOct
Press any key to continue or ESC to return to m e n u . ..
Title: FC-143 DAPHNIA MAGNA 21 DAY REPRODUCTION
File:
fc21d
Transform:
NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated Bl statistic = 6.7904
(p-value = 0.1474)
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
Critical B = 13.2767 (alpha = 0.01, df = 9.4877 (alpha = 0.05, df
4) 4)
Press any key to continue or ESC to return to menu... 003841
Title: FC-143 DAPHNIA MAGNA 14 DAY REPRODUCTION
File:
FC14D
Transform:
NO TRANSFORMATION
Dunnett's Test - TABLE 1 OF 2
H o :ControlcTreatment
GROUP
IDENTIFICATION
TRANSFORMED MEAN
MEAN CALCULATED IN
SIG
ORIGINAL UNITS
T STAT 0.05
1
CONTROL
359.2500
359.2500
2
5 MG/L
311.2500
311.2500
1.0073
3
8 MG/L
264.7500
264.7500
1.9830
4
13 MG/L
228.5000
228.5000
2.7437 *
5
22 MG/L
224.2500
224.2500
2.8329 *
6
36 MG/L
191.2500
191.2500
3.5254 *
7
60 MG/L
115.2500
115.2500
5.1203 *
Dunnett critical value = 2. 4600 _ (1 Tailed, alpha = 0.05, df [used] = 6 ,20) -> (Actual df = 6 ,21)
Press any key to continue...
Title : FC-143 DAPHNIA MAGNA 14 DAY REPRODUCTION
File :
FC14D
Transform:
NO TRANSFORMATION
Dunnett's Test - TABLE 2 OF 2
Ho :ControlcTreatment
GROUP
IDENTIFICATION
NUM OF REPS
MIN SIG DIFF
% OF
DIFFERENCE
(IN ORIG. UNITS) CONTROL FROM CONTROL
1
CONTROL
4
2
5 MG/L
4
117.2286
32.6
48.0000
3
8 MG/L
4
117.2286
32.6
94.5000
4
13 MG/L
4
117.2286
32.6
130.7500
5
22 MG/L
4
117.2286
32.6
135.0000
6
36 MG/L
4
117.2286
32.6
168.0000
7
60 MG/L
4
117.2286
32.6
244.0000
Press any key to continue or ESC to retu
003842
Title: FC-143 DAPHNIA MAGNA 14 DAY REPRODUCTION
File:
FC14D
Transform:
ANOVA Table
NO TRANSFORMATION
SOURCE Between Within (Error)
DF 6
21
SS 152796.3571
95377.5000
MS 25466.0595
4541.7857
F 5.6071
Total
27 248173.8571
(p-value = 0.0013)
Critical F = 3.8117 (alpha = 0.01, df = 6,21) = 2.5727 (alpha = 0.05, df = 6,21)
Since F > Critical F REJECT Ho: All equal (alpha = 0.05)
Press any key to continue or ESC to return to menu. ..
C03843
Title: FC-143 DAPHNIA MAGNA 14 DAY REPRODUCTION
File:
FC14D
Transform:
Shapiro - Wilk's Test for Normality
D = 95377.5000 W = 0.9434
Critical W = 0.8960 (alpha = 0.01 , N = 28) W = 0.9240 (alpha = 0.05 , N = 28)
NO TRANSFORMATION
Data PASS normality test (alpha = 0.01). Continue analysis.
AJ00L
Press any key to continue or ESC to return to m e n u . ..
Title: FC-143 DAPHNIA MAGNA 14 DAY REPRODUCTION
File:
FC14D
Transform:
NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated B1 statistic = 10.3740
(p-value = 0.1098)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 16.8119 (alpha = 12.5916 (alpha
0.01, df 0.05, df
6) 6)
Press any key to continue or ESC to return to m e n u . ..
GROUP
1 2 3 4 5 6
Summary of Fisher's Exact Tests
/O*.
IDENTIFICATION
NUMBER EXPOSED
NUMBER DEAD
CONTROL 5 MG/L 8 MG/L
13 MG/L 22 MG/L 36 MG/L 60 MG/L
20 20 20 20 20 20 20
0 1 4 4 4 5 16
SIG 0.05
* *
Press any key to continue..
\
^/c'f
G 03845
GROUP
1 2 3 4 5 6
m
Summary of Fisher's Exact Tests
IDENTIFICATION
NUMBER EXPOSED
NUMBER DEAD
CONTROL 5 mg/1 8 mg/1
13 mg/1 22 mg/1 36 mg/1 60 mg/1
20 20 20 20 20 20 20
0 0 0 0 0 0 1
,0 g l
Press any key to continue...
003846
ENTER THE NUMBER OF MORTALITIES AT EACH CONCENTRATION: 5 20 20 20 25 80
WOULD YOU LIKE THE AUTOMATIC TRIM CALCULATION (Y/N) ? Y
DATE: 8/7/1984 CHEMICAL: FC-143
TEST NUMBER: 1
DURATION: 21 DAYS SPECIES: D.MAGNA
RAW DATA: CONCENTRATION(MG/L) NUMBER EXPOSED: MORTALITIES: SPEARMAN-KARBER TRIM:
5.00 100 5 .
8.00 13.00 100 100 20 20
20.00%
22.00 100 20
36.00 100 25
60.00 100 80
SPEARMAN-KARBER ESTIMATES:
EC50
95% LOWER CONFIDENCE
95% UPPER CONFIDENCE
44.57 40.88 48.60
WOULD YOU LIKE TO HAVE A COPY SENT TO THE PRINTER (Y/N) ? I
's/ 4 Z&rv/Vtil
003847
Toxicant/Effluent: FC-143
Test Start Date: 8/7/1984 Test Ending Date:
Test Species: D.MAGNA
Test Duration:
14 DAY
DATA FILE:
8/21/198
Cone ID
Number Replicates
Concentration MG/L
Response Means
Std. Dev.
Pooled Response Me;
14 24 34 44 54 64 74
0.000 5.000 8.000 13.000 22.000 36.000 60.000
359.250 311.250 264.750 228.500 224.250 191.250 115.250
30.739 45.959 107.952 111.075 48.072 41.548 26.588
359.250 311.250 264.750 228.500 224.250 191.250 115.250
The :Linear Interpolation Estimate:
39.6711 Entered P Value: 50
Number of Resamplings: 80 80 Resamples Generated
The ]Bootstrap Estimates Mean: 39.1710 Standard Deviation:
6.7021
Original Confidence Limits: Lower:
27.6757 Upper:
48 .1897
Expanded Confidence Limits: Lower:
20.4784 Upper:
53 .3009
Resampling time in Seconds:
1.32 Random Seed: -309057854
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/Cp
Toxicant/Effluent : FC-143
Test Start Date: 8/7/1984 Test Ending Date:
Test Species : D .MAGNA
Test Duration:
21 DAY
DATA FILE:
8/28/84
/Ytf
PlA
Cone ID
Number Replicates
Concentration MG/L
Response Means
Std. Dev.
Pooled Response Me.
14 24 34 44 54 64 74
0.000 5.000 8.000 13.000 22.000 36.000 60.000
825.750 722.500 647.750 630.500 625.500 523.000 155.250
65.383 143.412 242.861 212.111
66.556 129.956
35.753
825.750 722.500 647.750 630.500 625.500 523.000 155.250
The Linear Interpolation Estimate : 43.1869 Entered P Value: 50
Number of Resamplings: 80 80 Resamples Generated
The Boot s t r a p 'Estimates Mean: 42.7226 Standard Deviation:
2.9224
Original Confidence Limits: Lower: 35.0020 Upper:
46.3442
Expanded Confidence Limits: Lower: 30.0910 Upper:
48.2386
Resampling time in Seconds:
1.26 Random_Seed: -523848694
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C 03S 48
