Document dQ3me8JQemE30JO7x8k7XL7Zb
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BIODEGRADATION (BOD/COD)
TEST SUBSTANCE________________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number is not noted. The test sample is FC-603EF. Current information indicates it is a mixture of 1.30% PFOS, 10% diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0% triethanolamine, and 0.05% tolyl triazole.
The following summary applies a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect degradation o f the fiuorochemicai component o f the test sample.
METHO D;_____________________________________________________
Method: Modified CBOD test Type: Aerobic GLP: No Year completed: 1996 Contact time: 28 days Inoculum: Polyseed-NX Lot # A306, Polybac Corp., Bethelem, PA.
RESULTS______________ !_____________________________________
Chemical Oxygen Demand (COD): Biochemical Oxygen Demand (BOD):
5-Day:
10-Day:
20-Day:
28-Day:
740,000 mg/kg
216,189 mg/kg 110,163 mg/kg (duplicate) 376,258 mg/kg 325,956 mg/kg (duplicate) 445,674 mg/kg 438,632 mg/kg (duplicate) 601,610 mg/kg 317,907 mg/kg (duplicate)
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fiuorochemicai proportion alone.
CONCLUSIONS______________________:_____________________________
002220
No conclusions given. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY____________________________________________________ Reliability: Klimisch ranking 3. Study lacks description and records of the method followed. The inoculum was not properly characterized. Sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number P 2 5 8 1 ,1996. O T H E R ___________________________________________________________ Last changed: 6/26/00
002230
S fe C O A /b R v N
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Modified CBOD Test 5,10,20 & 28 Day Analyses
I Rtt ^ ^ I
n a ta t \
Analyst ~T V -fi
Sample Description__iiliLL_ Concentration of stock solution J rl F ______ mg 1 L
pH Stock Solution
^ ___________
Polyseed -NX Lot # pv 6 O
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Modified CBOD Test 5, 10, 20, & 28 Day Analyses
Sample Description i
LR# Date
\ -
Bottle * Day
33 34
35
3G 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
t. 52
10
10
10 10 20 20 20 20 28 28 28 28
Sample vol.
(m jg/L L
Sample cone. (m q/L)
H .T f
9 .^ 4
9 .Y 49 >
4 44
H cf - >
44,4-
W <34.?
4 7 .4 -
49T
i 0 4 -7 4
<34.?
W-v: <'
vq -T
4 4 -4 -
4-9?
t( 9.44
:m i
best copy available
Modified CBOD Test 5, 10, 20, & 28 Day Analyses
Sample Description f: >' -<>'
LR# Date
v. , , . . '! ~fZ %
Bottle * Day
53
54 55 56 57 58 59 60 61 10 62 10 6 3 10 64 10 65 20 66 20 67 20
68 20
69 28 70 28 71 28 72 28
Sample voi.
(m g /L )
( Io
Sample cone. (m g/L)
H.fn cf.cm
S' l\7 .*
Initial Dissolved
Oxgyen (m g /L )
ci,ri q
^ 7 ?Hm
? .7 4
3S 7 ? .? H . ct 7
~9TFT
3HZ y 7 7 .>
Date
Initials
;-.-Su-' // -L'
.lU r* a j>2 u o I i - x i - i c, 11-21-7, i I -21-% 11-2.1-7,
~sj
1^3
TVC5 TV: 3
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Q r'X 'jv
iM l i.
L? -J* /-/z-? /<?-/2-9&
U -fi-*} t
&ST tv-<>
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s., , *,
V' V
&
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Product Testing
P2581 FC-603EF
Modified CBOD Test
5, 10, 20 & 28 Day Analyses
Mean CBOD m q/K q
216,189 376,258
CBOO Day 01 Bottle
Results
No. No.
(mg/kg)
0 1 33
0 2 34
0 3 35
0 4 36
235412 5 1 37
240443 5 2 38
191935 5 3 39
145070 5 4 40
301811 10 1 41
376258 10 2 42
Seed Vol (mL) 5 5 5 5 5 5 5 5 5 5
Samp Vol (mL) 5 10 25 50 5 10 25 50 5 10
Samp Cone. (mg/L) 4.97 9.94 24.8 49.7 4.97 9.94 24.8 49.7 4.97 9.94
Seed Corr. Factor
1.12 1.12 1.12 1.12 1.86
Sam.s ple"DO>D<;rop;S;i? Initial Rnal DO Drop 1 - Initial minus.,-:'
Dissolved Dissolved Initial minus ""c Final minusfetfy
Oxygen Oxygen;
' Seed C o rre s .
(mg/L) (mg/L) ' (mg/L) *5 1 ( M d i
8.45
8.46
8.46
8.46
L /,6.16
",-2.29
4.95
3.51
2.39
2.58
5.88
4.76
0.13
8.33
7:21
5 99 3.36
' ! 1.50
2.86
5.60
3.74
445,674 601,610 CBOD (mg/kg)
261290 10 3 43
5
130986 10 4 44
5
362173 20 1 45
5
445674 20 2 46
5
245968 20 3 47
5
123340 20 4 48
5
601610 28 1 49
5
510060 28 2 50
5
205645 28 3 51
5
CO CM
103219
4 52
5
( Samp DO Drop (mg/L) / Samp Cone
25
50 5 10 25 50 5 10 25 50 (mg/L)
24.8 1.86^ ^ ^ H
49.7 4.97 9.94 24.8 49.7 4.97
i '86lf ilS 8l l l
2`24S lfiilS fiS i 224 224IH IIM
224 S l l i i i l
9.94 3<24fiS i fiiflil 24.8 324 S ilS S ilii 49.7 3.24H I H
) X 1,000,000 mg/kg
0.12
0.09 4.41 1.79 0,12 0.09 2.22 0.15 0.12 0.09
8.34
8.37 4.04 6.67 ' 8.34 8.37 6.23 8.31 8.34 8.37
6.48
6.51 1.80 4.43 6.10 "'6 .13 2.99 5.07 5.10 ' 5.131
Greyed areas are data with unacceptable oxygen concentrations: Final D.O. must be > 0.5 mg/L, and Sample D O. drop (initial - final -seed correction) must be > 2.0 mg/L lor the data to be used to calculate CBOD.
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Product Testing
P2581 FC-603EF
Modified CBOD Test
Duplicate
5, 10, 20 & 28 Day Analyses
Sample DO Drop
In itia l
Final DO Drop
Initial minus ,
CBOD
D ay at B o ttle
Seed
Sam p Sam p Seed D is s o lv e d Dissolved Initial minus
Final minus
Mean CBOD Results
No. No.
Vol
Vol
Cone.
Corr.
Oxygen
Oxygen
Final
Seed Corr
mq/Kq
(mg/kg)
(m l)
(m l)
( m g /l) Factor
(m g /L )
(mg/L)
(mg/l)
(mg/L)
0 1 53
5
5 4.97
8.45
0 2 54
5
10 9.94
8.44
0 3 55
5
25 24.8
8.44
0 4 56
5
50 49.7
8.4p|
90543 5 1 57
5
5 4 .9 7 1.12
| 6)88
-*'1.57
105634 5 2 58
5
10 9.94 1.12
I" *-'is6.27 '' <?*P2)T7 1 ' ~'f >M ( 05
110,163 102419 5 3 59
5
25 24.8 1.12
4.78
3.66
2.54
\
117907 5 4 60
5
50 4 9.7 1.12
1.42
6.98
5.86
211268 10 1 61
5
5 4.97
5.54 - ' 2.91
1.05
186lllllfl!
325,956 325956 10 2 62
5
10 9.94
3.34 5.10
3.24
438,632 317,907
262903 10
130181 10 295775 20 438632 20 247581 20 122938 20 255533 28 317907 28 206452 28 101811 28
3 63
4 64 1 ; 65 2 66 3 67 4 68 1 69 2 70 3 71 4 72
5
5 5 5 5 5 5 5 5 5
25 24.8 i . a s i M i W 1 , ' 0.06
8.38
6.52
50 49.7
i-86IHHllfll5 4.97 2,24IHIHIilI10 9.94 2'24lilMiH25 24.8 2;24HIMNIHI50 49.7 2-24IBllSI5 4.97 3-24tlHiliIH10 9.94 33-2244SMSSHfilinSI25 24.8
1''M0.07 - 8.33
v'i5^4.74 .`.'eU- 3.71
1.84
6.60
'*^0.06 ' ' 8.38
' ' 0.05 ' 8.35
3.94
4.51
2.04 6.40
0.08
8.36
' 6 .4 7
' U * '^ *1 .4 7
4.36 ''6 .1 4 ' ' r -`e / n
1.27 3.16 5.12
50 49.7 3 . 2 4 H S n H
0.10
8.30
' 5.06
CBOD (mg/kg) ( Samp DO Drop (mg/L) / Samp Cone (mg/L) ) X 1,000,000 mg/kg
Greyed areas are data with unacceptable oxygen concentrations: Final D O. must be > 0.5 mg/L, and Sample D O. drop (initial final -seed correction) must be > 2.0 mg/L for the data to be used to calculate CBOD.
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Modified CBOD Test 5, 10, 20, & 28 Day Analyses QC Data
LR#
P a 9S?
D ate ,jTfg.T&o 11-2.x ______
A n a ly s t g j j | r \cs________
Sample D escription
Medium Blank Medium Blank Seeded Blank Seeded Blank Ref. Sid. GGA Ref. Std. GGA Tox. Check LR# *.25* I Tox. Check LR# Tox. Check LR# P245Z Tox. Check LR#
Day 5 Medium Blank Medium Blank Seeded Blank Seeded Blank Ref. Std. GGA Ref. Std. GGA Tox. Check LR# P 2 5 8 I Tox. Check LR# P M 5 b Tox. Check LR# P A 95 8 Tox. Check LR#
Dav 10 Medium Blank Medium Blank Seeded Blank Seeded Blank
Medium Blank Medium Blank Seeded Blank Seeded Blank
Day 28 Medium Blank Medium Blank Seeded Blank Seeded Blank
Bottle
1 2 3 4 5 6 7 8 9
10
In itia l D is s o lv e d
Oxgyen (m g/L) '<. W s 'K .H t
'f.-YS
K.H 7 ff.v y H ML, *0c7 Q.\j O
Final Dissolved
Oxgyen
Dats
In itia ls
.IL M M .
lh2JL-9<r
//vU -Pfr I/-2J -9 fr
J t& .-ik .
J&5L jSL
j5 _ B<U
6-33
63J JT BJT
-3 . L
11
S .o H
' -A i-lto
Tvcs
12
%. 3
li -x i-9 4 ,
TbS
13
-7.-L7.
li - x i-9 (
TIcS
14
11-X T -9 &
T<cS
15
ssh
II-X T-9 b
Tks
16
S .lT .
l\ -x -r-'tb
TkS
17
O. 11-
11-7 .1 -9 (o
T ys
18
O .IS
H -37-9C . - U r f
19 1 O . f.2, 1 I-Z 7 -Y C ,
20
1 uot eo
--
21
r
12-2-9^
3ST
22 v i a
23
1 to. wG -
6
24
1 U. ottf
. . . ____
25
S -05
/ A - l X-?t.
T<s
' m e*
26 ! 7.0 <,, la - r x - i t ,
27
4..7.H / J - t X - Y fa
-iV<LS
28
Co - 2o '-a - 1 1 - 9 6
TVS
29
7 .* 1
7 2 - 3 0 - 7 b T V S ` -
30
7 TM ^ /j
-v`Tfc-5 ' ' .
31
1 'ff.fc
/d? - a jo - Y b - < T t 5
32
5 i *b '
^V -5
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Modified CBOD Test 5, 10, 20, * 28 Day Analysas QC Results
LR* Date Analyst
^ P "S 3
+ 7 I I - 2. -9fo A _S
v ______________
Description
CBOD mg/kg
B o ttle no.
In itial dissolved
oxgyen
tTM g 'U
Final dissolved
oxygen
5 Day QC Medium Blank Medium Blank Seeded Blank Seeded Blank
1 1 8.46 12 8.47 13 8.47 14 8.45
8.04 8.13 7.22 7.47
Ref. Std. GGA Rof. Std. GGA Tox. Check LR#P2581 Tox. Check LR# P2956 Tox. Check LR* P2958
298 326 >1000 >1000 >1000
15 16 17 18 19
8.45 8.47 8.47 8.47 8.44
5.84 5.72 0.17 0.15 0.13
DO drop In itia l'
Final
(m g'-)
DO drop In itia lF in a lseed cor (m q /L )
0.42 0.34 1.25 0.98
2.61 2.75 8.30 8.32 8.31
mean SCF= 1.49 1.63 7.18 7.20 7.19
SCF* 1.25 0.98 1.12
10 Day QC Medium Blank Medium Blank Seeded Blank Seeded Blank
I H H H
20 Day QC
Medium Blank Medium Blank Seeded Blank Seeded Blank
1 1 1 |
28 Day QC Medium Blank Medium Blank Seeded Blank Seeded Blank
1 1 1 |
"(Seed Control Factor)
21 23
24 25 27
29 31
8.46 8.47 8.47 8.45
8.46 8.47 8.47 8.45
8.46 8.47 8.47 8.45
8.13 8.12 6.65 6.56
8.08 8.06 6.24 6.20
7.81 7.84 5.30 5.15
0.33 0.35 1.82 1.89
0.38 0.41 2.23 2.25
0.65 0.63 3.17 3.30
SCF* 1.62 1.89 1,86
SCF* 2.23 2.25 v2.24
SCF* 3.17 3.30 3.24
"X; t
's
Tox. Checks contains sample at the highest cone, used for dilutions & 1.5 mLs of HACH GGA Std
Ref. Std. BOD (mg/L)=(Ref. Std .DO drop-Seed Correction Factor) X {300mL/ Ref Sid voi (ml)}
