Document dQ0N0LLBneV94Q6Em174pj7VG

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:A mixturecontainingperfluorooetanesulfbnatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component of FX-3103. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M producton lotnumber was notnoted.The testsample isFX-3103, 3%. Currentinformatioinndicatesitisa mixtureof0.96%PFOS, 20% diethyleneglycolbutylether,50.56% water,15% ethyleneglycol4,.32% sodium octylsulfate6,.0% polyoxyethylenemonooctylphenylether,2.5% propane sultonefoamer,0.5% sodium nitrate0,.06% sodium laurylsulfate, and 0.1% benzotdazole. The followingsummary applies toa mixture with incompletely charactefizedconcentrations of impudties. Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: Not given. Type: Staticacute GLP: No Year completed: 1985 Species: Daphnia magna Supplier:In-houseculturesO.riginalloybtainedfrom U.S.EPA-ERL, Duluth, MN. Analyticalmonitoring: Temperature, pH, and dissolvedoxygen. Exposure period: 48-hours Test organism age: <24 hours old Statisticalmethods: EC50 values calculatedusing logistircegression methods (probiatnalysis). Test conditions: Dilutionwater: Carbon-filterweedllwater. Dilutionwater chemistry: Not given. Lighting:Not given. Stock and testsolutionpreparation:A primarystocksolutiownas prepared by dissolving1000 mg oftestsubstance in500 mL water. Exposure concentrationswere preparedby additionofstocksolutiontoeach vessel. Exposure vessels: 250 mL glassbeakers containing200 mL exposure solutionata depth of6 cm. Number of replicates:2 Number of daphnids per replicate:10 Number of concentrations: fiveplusa blankcontrol Water chemistry during the study: pH range(0-48houm): 8.1- 8.2(contreoxlposure) 7.8- 8.1(1000 mg/L exposure) Temperature range (0-48houm): 22 OC Dissolved oxygen range (0-48houm): 8.9- 9.3nig/L(controelxposure) 7.0- 9.3mg/L (1000 mg/L exposure) RESULTS Nominal concentrations:Blankcontrol1,00,180, 320,560, and 1000 mg/L. Elementvalues: 48-hourEC5o=>1000nig/L Element valuesbased on nominalconcentrations. Remarks: Testingwas conducted on a mixtureas describedintheTest Substance rrmarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 48-hour EC50 was determined to be >1000 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 3. Study lacksdescriptioannd recordsof method followed.The studylacksinformatioonn purityofthetestsubstance and analyticaclonfirmationofthe amount offluorochemicalproportionin solution. REFERENCES Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Cl I11-1,1985. OTHER Last changed: 6/26/00. ENVIRONMENTAL LABORATORY LR AQUATIC TOXICITY DATA SHEET TEST TYPE @ CHEMICAL TESTED LOT TEST ORGANIS SOURCF/t/4aL-,kEZ2 2 DATE REC,N AGE OF TEST ORGANISMS:/, 24 hrs. old instars (neonates) NO. ORGANISMS PER TREAT!4ENT 'AM . TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200ml D 6cm TIME STARTECL DATE STARTED-..21-9@41R, TE COMPLETED-- DILUTION WATER Carbon-filteredwell water (Temp.2,,"') , D.O. pm. pH ANALY-- EC 50 (95% Confidence Limits) TESTII.itial Total VESSE Com. Amcxmt NO. mg/l Added 24 Hours Total No. pH Dead 48 Hours D.O. Total No. pH ppm Dead D.O. PPM L Contra] 0M , -x2n 4 C)1 If)f-Y-I)cn COMMENTS: Test Teny. OC '=tlals & Date OKI SECONDARY EFFERT'Z .-TEST vrCS.SEL Nn Dontrol Symptans or Peactims TOT@I _HNRUMBER EFFJ@T ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET TEST TYPE -CHEMICAL TESTED c5t@'@/ LOT -- TEST ORGANISHL ---7--- -- iPfa#64SOURCr--//,22@g=CZCt.- DATE REC-0. Zc'Z@ AGE OF TEST ORGANISMS:,424 hrs. old instars (neonates)-NO. ORGANISMS PER TREATMENT W94M TEST VESSEL: 250ml Pyrex glass beaker TEST SOLUTION VOLUME: 200ml DEPTH-.6cm TIME STARTED DILUTION WATER Carbon-filtered DATE STARTEN .2@k f . well water (Temp. :@?2 n TE COPOPLETEn.c2-@4@4/Ri.C &41 z D. 0. ppm 9 PH_&.,.Lj c60 (95% Confidence Limitsl TES@Tiniti;kl Total 24 Hours VESSE cow. PharnoLmmTtotal No. pH NO.1 mg/i Added Dead 48 Hours D.O. Total No. pH prm Dead D.O. an Contiml 0 ;zr4 COMMENTS: Test oc Initin' Date SECONDARY EF Ir VE:@5EL -M" TOT@i_gr@MBER EFFI@T Symptans or Reactions, @ontrol INVIRONMENTAL LABORATORY TABLE.1, SUMMARY OF ACUTE TOXICITY OF TO DAPHNIA MAGNA UNDER,..- ,41 ZXPOSURE CONDITION CHEMICAL CONCENTRATION 24-HR. PERCENT MORTALITY CONTROL Ion :2rC) I()C) 0 4-0 Ec (95Z CONFIDENCE 50 (2) LIMITS) > IC)C)() (1) Data were averages of two replications for each concentration with ten replicate. A total of twenty daphnldo were used per concentration. -.--(2) zc 50 values were calculated using logistic regression methods (Probit An