Document dDxjJpVZjjZXbxXxXX7vpeM55

A R # 6- o m it ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: [2-(N-Ethylperfluorooctanesulfonamido) ethyl acrylate: may also be referred to as B1228, D-1, EtFOSEA, or FX-13. (2-Propenoic acid, 2[ethyl[heptadecafluorooctyl)sulfonyl]amino]ethyl ester, CAS # 423-825) Remarks: Material is an amber solid. Sample purity and not recorded. 3M lot number 13. METHOD Method: Protocol Reference EAR/WAS 5/9/84 Test type: Static acute GLP: No Year Completed: 1984 Analytical monitoring: Dissolved oxygen concentrations. Test organism source: Activated sludge collected from the St Paul/Minneapolis, MN Metro Wastewater Plant on 5/10/84 Test conditions: Dilution water: Deionized water Test solution preparation: Placed 0.30 g test substance in a 300 mL BOD bottle with 50 mL deionized water. The mixture was mixed with a magnetic stirring bar and box for ~ 30-minutes in order to disperse sample. After mixing the sample was finely suspended in the water. Test vessels: 300 mL BOD bottles Number of concentrations: One Temperature: Not recorded Total Suspended Solids of sludge on day of testing: Not recorded. Sludge was used the day of collection. Exposure period: 6.65 minutes Element Basis: Respiration inhibition as determined by dissolved oxygen concentration. RESULTS Nominal concentrations: Two blank (deionized water) controls, one 1000mg/L test material solution. 000541 Statistical Analyses: EC50 values was not determined as the oxygen depletion rate for the controls was judged to be equal to that of the deionized water, controls Analytical Methodology: Method for the analysis of dissolved oxygen concentrations was not recorded. Control response: Satisfactory: difference between controls was 6.9%. CONCLUSIONS The test substance exhibited a no inhibitory effect on respiration at a nominal test substance concentration of 1000 mg/L after 6.65 minutes of exposure. The EC50 (respiration inhibition) is therefore greater than 1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. This study was not conducted using an established method. Testing was also done well above the solubility limit of the test substance. This study lacks sufficient documentation and characterization of the test substance purity, and analytical measurement of test concentrations. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number B1228, 4/1/84 OTHER Last changed: 5/22/00 000542 3M Environmental Laboratory Acute Toxicity of a Substance to Activated Sludge Mcxed Liquor (SLUD6E~T0X) Protocol Reference* EAn/WAS 5/09/84, Lab Request No. B1228 ST0X.DAT TIME - MINUTES 000543 BEST COPY AVAILABLE 3M EnvIronmenta1 Laboratory Acute T o xicity of a Compound (Substance) to._Acti vated Sludge Mixed Liquor Protocol Reference* EAR/VIAS 5-09-84 ' .M - 77/Lab request no.: JL22,L, Date Analyst:' The activated sludge mixed llquor.was collected from the S t. Paul/MInneapolIs Metro Wastewater Plant on - '7 " . / t---------- Sample description: ( -------------l- )----------f-~--^-----^--- y , -- L----------t--y/ ----f---------------)-j---,---L--o--T--~----)- 3 Preparation of stock solution: grams of sample were d ilu te d t o ______ _ ml with deionized reagent water. Pate of preparation; > t-c t im e -------- . HCE.NTRATION OF TEST MATERIAL LQ00_jy\yk MW AJ7YImin. sec. C?(lTR&L ATEO SLUDGE C MIXTU*> R^E AVC^-(4Ci C 7 ^ 7,i l. f / -- 7*/r 7 Jo s' 6,/r t,oo 7 .r r 7 ./T x/S'' *7.^T^ 17 ? , i f ,3 - <X g7 9 i< r 7 /O iT-of y- 5" - r b` ,7 3,SV 3 7 o J< rr -- !,2S^ * 777 -3 p X Alt v\(vt xT,cr,/n\ Cp -11( iy,v 2.?<r- /. 6 3. > .rr" 1.9 2.7ZS~ , ! y 7J- (>*^ yv \ X d tF \ (p>-/ -rxTw- lX n 1 ^ X COMMENTS: ~T-i<sL C\ 300 y*X A^^Ji Mry~ Tb. p i_ AsnA^. * 5^ >0^v>X"Z Ov<Llj -- =.---- ~ 0>O 4str?lyl' yrssy i- r i _ < r ^ - r 0 .-- _ r~ ,^ Xc' x I k 000544