Document d33VVrX0KxOpY7zMKkR60gnb
ACUTE TOXICITY TO AQUATIC INVERTEBRATES TEST SUBSTANCE
(DAPHNIA MAGNA)
Identity:A mixturecontainingPerfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-206A. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was cc 785-22. The testsample isFC-206A, an amber liquidC.urrentinformatioinndicatesitisa mixtureof 0.92% PFOS, 18.75% diethyleneglycolbutylether,75.44% water,1.83% Sultonefoanier,1.75% sodium octylsulfate1,.2% poloxyethylene monooctylphenylether,0.06% sodium laurylsulfatea,nd 0.05% benzotdazole.
The followingsummary applies toa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemkal component of the testsample.
METHOD:
Method: Not given. Type: Acute static GLP: No
Year completed: 1979 Species: Daphnia magna
Supplier:In-houseculture.InitiaflrloymOSH Wis.
Analyticalmonitoring:Temperature,pH, and dissolvedoxygen. Exposure period:48-hours
Test organism age: Notgiven
Statisticamlethod: Probitanalysis Test conditions:
Dilutionwater: Carbon-filterweedllwater
Dilutionwater chemistry:
Hardness: Alkalinity:
236 mg/L as CaC03 221 mg/L as CaC03
Conductivity: Lighfing:Not given
430 pmhos/cm
Stock and testsolutionpreparation:The testmateriawlas added directly tothe dilutiownater on a weight/volume basis.
Exposure vessels: vesselswith100 mL exposure solution Number of replicates:2
Number of daphnids per replicate:20 Number of concentrations: fiveplusa blankcontrol
Water chemistry during the study: pH range (0-48hours):
8.0- 8.0(controelxposure) 7.6- 8.0(1800 mg/L exposure) Temperature range (0-48 hours): 19 - 20 OC Dissolved oxygen range (0-48hours): 6.9 - 8.5mg/L (controelxposure) 4.9 - 8.5 mg/L (1800 mg/L exposure)
RESULTS
Nominal concentrations: Blank control5,60, 750, 1000, 1350, and 1800 mg/L.
Element values: 48-hour EC50 = >1,800 mg/L (Replicate1) 48-hour EC5o = >1,800 nig/L(Replicate2)
Element values based on nominal concentrations.
Remarks: Tesbng was conducted on a mixtureas described inthe Test Substance Remarks field.The values reportedapplyto thatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsample 48-hour LC5o was >1,800 mg/L, the highestconcentration tested.The combined percent mortalitfyorthe replicateast thisconcentration was 27.5.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability:Klimischranking3. Testinglacks record of methodology used. There isa lackof characterizatioonfthe testsample and the study lacks analyticaclonfirmationof the amount of fluorochemicalcomponents inthe solution.
REFERENCES
This study was conducted by the 3M Company, Environmental Laboratory, St.Paul, MN, Lab Request number 4970S, 1979.
OTHER
Last changed: 6/26/00.
ENVIRONNENTAL LABoRAmRy AQIATIC MXIClri DATA SHEET
L-k 41
Type Test Test Organis Avg. Wt.
talho-e- Material
gram
c Avg. Size
Tested VC. - -Z A
loot
Date Recid._!g-11-liBatch No. CH No. Organisms per dose
Test Solution Volum 4A*4o@rs/AIl-Glass Tank Tank Dimensions.--36*3&-ea-and 24-cm-d"p
Time Started
b oo, Date Started
-9 Date Completed
7-14 -7t
Dilution Water Carbon-filtered.welwlater (Temp. ZD Oco, D.O.
;Avostigatorvv-,.@k..,.cltyh3w,4 Signatu")
]cc . so
(9S%
Confidence
Limits)
=n Conct_ .. Total Tmk Conc.
--N Fours
48- urs 0.1
72
Amount Total
D.O. Total
D. Total
Added No. Apad i)H pps No.Dead DH inns No.Dead
Control
t) %P -7.2-
96 90, Total
Do ad
4.9
Test Temp. Oc.
S-ioature & Date
Comments:-V
a
Effects other than Mortality
in c orIkc. M)-
Symptoms or Reactions
Control-I
24-Hr.
EC so
Total No. Affected
48-Hr.
72-Hr.
96-Hr.
Signature Comments:
Date
ENVIRONMENTAL LABORATORY AQIATIC TOXICI7Y DATA SHEET
q
---------
Type Test Test Organism Avg, Wt. Test Solution
&&eae Shxcma torial Tested
- -2c,6 4 L&t
urce
s @l
Date Recid.-4---U--I-Biatch No.
gram Avg. Si ze
04 No. Organisms per dose
Volume 4.,o ict\../All-Glass Tank
Tank Dijwnsionst 36x2l en
GA4--U
0
-em e-feep
Time Started X()'.#7 C) (A--%Date Started
'7 1-2 -9
Date Completed
'7-
Dilution Water Carbon-filtered.well
@nvestigator (Full Signature).
water (Temp.
0 1> C., D.O.
fic
S6
(95% Confidence
Limits)gaff
-ppe, piL 4E. 6
t.
Tm*
OUC.
mg/l
c ontrol
Total. Amount Added
24, urs
Total
D.O.
No. Dead I)H pps
t,
48 Total No.Dead pH
D.O. pps
0
E.o '2.z
Tot al No.Dead
HdG; *00 ims
96 -H Total.
DoadOTH
0
I-006.-
too
0
*7 5;.1
r,
@.Lfot)-
o
-7.7
Test Temp. 0
Signature Comments:
Date
istC"-Z@,
-P
-7--1 e4
Effects other than Mortality
Conc. .2m@l
Control
Symptoms or Reactio ns
24-Hr.
EC so
Total No. Affected
48 Hr.
72-Hr.
96-Hr.
Signature Comments:
& Date
3M -EfiVIRONiIENTAL- -LABORAT13Pi
*O*f%DDIT -PMAL-YSTS -FOP -PTORSSAY -DRTA*o@w
NO 'OF 'CURVES --1-4 NO.--OF -CONC.- FOO -CUOVE -1 4 INPUT -DATA-CONC..P"().--ExPOSED,ma.-riFAD
-560rl6rO .75ort6ri -tOOOrl-6r2 -1350@t6rtt
COMME-"TS -FOP -CURVE -t
-FO.
-FI-S
.. .........I....L.as ......... Ttt)M.....F*V'FED ODMO .........PE.S.PUMDI"G ...F.PRCITIOM
-560 .........2.@7.48tgS .....0............O.w.OOtt3O39*-7"3.0........a....87506t .....0..-0625 .......O..-02n$678 -kooo .......... 3 ...........0...k.e,.5.......0..-i?kt%92 -k35O ........3...-S-30334 .....O..."?s .... O.-6432t2@ CHE-90;PPODRDTLFT-VtS->-0.-0-3
........IK.TC-PO T ....S.LOPE ........CH.I -SO -FMR -'-014t.90 -Fi3P ...........(9.).@.......L.t."EAPtTY ....F.IPGBRBILITY
......
-t@(POSI-48 .....0..-44;1!502-
.@.:@@@IETV
-FMTOP -ON>
........
t229.-363
t .............6 .--.-0.-2756303
95 -PCT. -L-OWEPt-aw.. -LIMIT ...............t.O.9.9w32695 -POT.-UPPEP -COW.- T-IMIT ...............k.49.5..-OE3 -
4MM. 4MR <XWIPE '-2--7 .5 f?WDM4t.-DEFID
-730,*6,2 .*eoort6r2 kg5o,*6,to
OCM"ENTS 4MR IPJPYE -2 -FO -L'068 -FISH
............l.@.W . OOMO,@ .........
-f:IkFiO1T,M .....flt
:'-420 ............2.423e4g> -- -o ...........0.-.Oow:@9 .560 .........2..@.749tee,:,. -,,o@-06n .......0.-.02etn4 -750 .........2...-.SM063 ......O.etn ........0..-*0593"'.....................0.e.k.L"5........0.,2.749694.e-
k358 .........@.ie.l-30334
-0.,625 ........0..'53e4-095
-7'CHI-Sg--PROBADtLITY
-IS
NO -OF
-INTERC4-'-PT
SLOPE ........@ONT@SO4@W ...C.HI- -SO -FOR
@KTEP IONS ....(.A> ......... .........L-.I.!iEaPtTY ...44KWDtLETY
@.5 ..........-t.6.e2l-943
--5.-2071-92......
HEF
ItTy ~TW
.(N)@Iiit .............6 .-n.i,-Ok2767633 -
-1-302.405
95'@@MT.@ UPPEP'404pe
T. .............
L
NO.- -OF -COW.- -FOP C(AzW .3 .3 INPUT -DATFo--Como,,Ho..+NMSED,h[),DEAD .?.koooreo@o -?-*35Or2Ork .?-LSOoreo@6
OOMME"FS -FM -OUPW -3 -? -DFPMIA -FO -eo6A
......I .......L-.06 ........F.W.FlOti .....r.-IT-'FC-D OOKO .........O.GN.O .........R.ES.PONDTHG ...F.PAOT-10
-tooo -t350
.................3..............0..............o..-.ool-7
3.-k3O334 .....0..-05 ........o....04.0,
-tooo ........3...-255273 .....o.e3
....O.e3O34
ONI-50 -PMBABILITY -tS -0.-05
MG-OF ...........IN.(TAE>R.01.7T ....S.L-DPE ........ON.I -90
IPE-RATIONS
(9> .........
-6 ..........-.3k.-3"79 .....9...459595
METEPOGEMIETY -FK-TOP -(H> LC-50 .........2".o..1.6k
............6.-.-..0.4
95 'P()T-.L-OWE-RC-ONF-EL-fM-taT.............1...7s.e..e 92 @OC-T -'-UPPER -COW,- -LIMIT -0 .............-.5.36.4wSS C'-G-'-OF -CONC.- -FOP -CURVE -4.--1 .3
INPUT -DATA-COK-.-,ND.-.,*XPOSEDM.-DE,9D -?-kOOOr2OrO
'?'t35Or2Ort ...teooraor5
C'@""E'MT-9 'FOP -CURVE -4
-DAPHNTA -FC -206A
.............L-.06.... ....FPACTIUM .....F.I"ED ODt4C .........0.'.3h.O........M.SP.O"DTM ...f:RW'FI-M
-tooo ........3.. k350......
.......0.............C...-89e4
-3.-k3O334 .....0..05 -kooo........
........O...-0430(pe
.; .,,**..f.t.........
-O.-OS
y .............
14ETERME"tC-'FYfk.IM .(">
L-C--50
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