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AR226-3064 DuPont HIiR 904-96 ttxaggT.T. LABORATORY FOR TOXICOLOGY AND INDUSTRIAL HKDICINE STATIC, ACUTE 96-HOUR SCREENING TEST OP H-21954 TO FATHEAD MINNOWS, Plmaphalas proaalap PURPOSE: This is a rangefinding study designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. GENERAL INFORMATION MR: HASKELL NUMBER: TEST CODE: SPONSOR: NOTEBOOK ID: SUBMITTER'S NOTEBOOK NO.: MATERIAL TESTED: SYNONYMS: COMPOSITION:' CONTAMINANTS: OTHER CODES: CAS REGISTRY: PURITY: TEST CONDITIONS Noainal concentrations tasked: 0, 0.5, 1.0, 50, 500, and 5000 mg/L. i Page l of 2 Company Sanitized. Does not contain T SCA CB DuPont HLR 904-96 waarnt.T. LABORATORY FOR TOXICOLOGY AMD INDUSTRIAL MBDICINB STATIC, ACOTB 96-HOUR SCRBXHIHO TRST OF H-21954 TO FATHRAD MINNOWS. prom!*. CONTINURD TRST CONDITIONS (Continued) Dissolved Oxygen end pE: Nomine1 Concentrt ia> (mg/L) At test initiation, 0 Hours Disp^lvad Oxygen pH (mg/L) 0 0.5 1.0 50 500 5000 8.4 7.5 8.4 7.5 8.4 7.5 8.4 7.5 8.4 7.5 8.5 7.6 At test completion, 96 Hours Dissolved Oxygen pH (mg/L) 7.4 7.5 7.6 7.4 7.2 8.4a 7.6 7.6 7.6 7.6 7.6 7.6a a Reading taken at approximately 3 hours after exposure, due to total mortality. RESULTS Based on visual observa* : 5ns. all test solutions were clear with no color, except for the 5000 mg/u test solution, which was cloudy, white in color. The 5000 mg/L test solution was cloudy, white in color. Mortalities were 0, 0, 0, 0, 0, and I00r;, respectively. The 96-hour LC50, based on nominal concentrations and mortality, was between 500 and 5000 mg/L. All water quality parameters were within acceptable limits. CONCLUSIONS H-21954 exhibited low toxicity in a 96-hour, unaerated, static acute test using fathead minnows, Pimephales promelas. WORK BY: nes w. Wetzel bxicology Associate Mo/Day/Year APPROVED BY: Michelle R.^rown, Study Director Mo/Day/Year DISTRIBUTION: 2 Company Sanitized. Does not contain TSCA C61I