Document bye5y103Q5eprmBQzkwr4G721

A R M 6 - 07S X ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 519. The test sample is FC-203CE. Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene monooctylphenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. M E T H O D ________________________________________________________ Method: OECD 209 GLP: No Year Completed: 1989 Analytical monitoring: Dissolved oxygen concentrations, pH, and temp. Statistical methods: Results were determined by calculation of the % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro W aste Treatment Plant, St. Paul, MN Test conditions: Dilution water: Millipore Miili-QTM water Synthetic sewage: per OECD method Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10 mL 1N NaOH, diluted to 30 mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1N H2SO4 and diluted to 1L with Milli-QTM water. The pH of the reference solution was measured to be 7.8. Test substance was added directly to test vessels. Test vessels: Not given. Number of concentrations: 5 plus 1 reference substance and 2 blank controls Temperature: 1 9 - 2 1 C Total suspended solids and pH on day of testing: 4.1 g/L (TSS), initial pH 7.2 and final pH 7.5 Element Basis: Respiration inhibition as determined by oxygen consumption. 001107 Rem arks: Values corrected to 20 C for calculations. RESULTS________________________________________________________ Nom inal concentrations: 2 Blank controls, reference substance, 100,180, 320, 560, and 1000 mg/L Elem ent values: 30-minute EC50 = >1000 mg/L 3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The test substance 3-hour EC50 for activated sludge respiration inhibition was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ R eliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES______________________________________________ ___ This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number G 1 3 5 8 -1 ,1989. O T H E R ___________________________________________________________ Last changed: 6/26/00 001108 ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR lr. 6 .L & 5 Z M ., Date to - g ? Analvat 5-Xfcy Type Test O jsCX> A r i \/aJrecL ^ d n r h ? . &CLpi'rdL+lnn Tin h i L v4<OMy # LGCf Source h/\o\rr> ll )a c-k? lfratr& h(- V'ldJri- Q - ______________ Date Obtained fo -*? ________________ Date Used & ~ *7 Mixed Liquor Suspended Solids (MLSS) / g/L Adjustment A Jo n f g/L PH T n . W - 7.X- Ft'n/d -- 7 -S~ Comments.______ Test Substance Sample Description F . - % 0 3 T lr ,5~7 ^ Sample V^M^neSt CoriCj J C 0 ^ .= 7 2 Adjustment Commente__________________________ _____________________ Reference Substance Sample Description 3 , ^ - h lo m p h e n o f_________ Sample p H / f o r L 7i Adjustment A/ott* Comments ________ Exposure Conditions Test Duration 3 ^ $ 6 f^ Test Temperature lQ ~3i I C eoamente fteurt U-llH-U; t-t. G \ Z S l - Q 001109 Fofm 10M O O PW O Sample Description F C - A o Z C i; Lc+SI<\ Lab Request No. <S 3 & - I Environmental Lab -- Work Sheet OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 Pafle------- of Date: ____ b Analyst: -- ^ ---------- ^ ------- Test Solutions Preparation. Test Cone. mg added ml DI water ml syn. sewage ml microbial ^CnfcT tfon+rok lt>o lSo 3 o 5 bO loop -- ,5o 7o IloO + 80 4 SOO _L_ A2H 8H 8H ASH 4'b 4t t' l b 4l b 4r~ )b h lb f i b fl b _____ f - A oo Zoo Zoo Zoo Zoo Zoo Total volume ml - 5oo - S~oo - Soo - S 'o o - S~oo - Sco b i+ J L -p tk Cong. / o o n n y ^ / t y -r=-.ZiA O O lllO ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST RAW DATA #209 LR G t 3 S % - \ Test Substance: PC. -- / 2 7 C _______/ - o f , 5 7 7 Source of Activated Sludge Mixed Liquor: _ A fc + fO UJa Tfe a + ftvM F}ar\-b Aa AJ Date Receive!;.U> - SH Date Used; L -7 __________ Analyst BEST COPY AVAILABLE 30 - MINUTE EXPOSURE lest substance Concentration (mg/L) CONTROL I Mo IS O te n . S io /DOO - r m MINUTE5--- 0.5 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 7 - 9 7.6 7 . 0 (o T .O 5 r f.O : 5 5 6>- U .9 . 3.5 . 0 f . f 3 - 1 " 3 , 5 5 ? A.3 /. 7 5 7 .D A 3 5 , 1 5.0 l A 3 7 3 . 1 /I 1 .3 s < \ 5-/ H-H 7 2 .1 /$r L >2. 5 - 9 H A H -o SO 3 . \ /.A * .* / sfctHs L > & /.L? 3,| < 7 3 /- .7 TEM 10.0 C* 5 .o Z L h 3 2 90 ZC 7.0 1 CONTROL II 7 .2 7.0 U S 54 5 y 5 3 3. 5 . 3 i n 7,2 2 L 3 - HOUR EXPOSURE lest Substance Concentration (mg/L) CONTROL I 0.5 1.0 7 $ 7.A DISSOLVED OXYGEN CONCENTRATIONS mo/L TO INUTES 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9 .0 10. TEc*0 7.0 ..5" ,P 5 5 Y,^ 5 3 3.3 /D O } D 3 o 5~6 D D O 7 A 3 5. A .57 I X 3.7 3 .2 A Z 4 * 55a ,3 ft.? ft.l Z 7 7*3 5 5 n 3,7 17 ,b ft.? 7 # 7.Y 7.1 (e>. 7 5.3 5~,^ s - . f ? 7 3 7-3 7 ^ .5 ,.9 .5 y >7 7 -le 7. V lp & 3 5.g 5 .0 . 5.7 33 5 .I -V.b Al CONTROL II 7-3 7,1 L*1 53 Y5 A l 3. 3 .7 2.1 pH of Activated Sludge Mixed Liquor: pH of highest concentration tested: Test Temperature: Initial 7 . ^ Initial Final 7 5 ^ Final 7 o 001111 L ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS Test SiihstanfP FC, ~~ f ^ 0 3 0 ^ - 30 - MINUTE EXPOSURE Test Substance Concentration (mg/L) RlfflroWflHHHHilHi 10 0 /S o 3^o ..... 5 ( o O . LODO Oxygen Uptake Rate R <9 . sn<=> < r O .S 3 & & 0.-&333 0 1 73 0 ^ 3 HR 0-8137 R Corrected^ to 20 C o.so.S-tr- 0 . 3 S ? >.>333 0 . ia im 0.9>5\<\ V LR O J S B z l Date fc>-* Analyst 4 Inhibition in , 2\ Respiration Rate1^' -- -- -- -- o.S S H S ' Percent Difference in Controls (3) ECgQ (951 Confidence Limits) TMg/T 1 , 0 *7c ^ /O dO 3 - HOUR EXPOSL Test Substance Concentration (mg/L) Oxygen Uptake Rate R 100 i& o ,, mo 3 LO ! Ooo 0, o .s m O . 3 c u ,,l\ >. 3 # b o 0. a .^337 0-31 n 'f R Corrected to 20* C O . 5 o n to o .G ia i o .H is o 0 , 3 <*S~ 0 - 1 4 Inhibition in ^ Respiration R a t e ^ J -- 1 .n3io ( ,.i, A3... AS A.O CONTROL II_____________ __ Percent Difference in Controls ECrn (951 Confidence Limits) mg/L , /DO (1) Temp. Corrections. -- er (mg 0-/L-min.) 1 /RC, + Rc \ (2) 4 Inhibition ZV* * 3 5....7X - 10"0s Where: R Oxygen uptake rate (mg 0 2/L-min.) T,obs Sample temp., C* / * - 1.047, x - T,obs - 20*C C Control o .5 3 o q 1 (3) 4 Difference - I i X 100 (RC, + Rc\ rn The two control respiration "Tates should be within 151 of each other S- p ^ d . -ior +*&, p r^ p . 001112 ODD 10830 0 PWO ample Description }S~- l>cU|oropKehol -e.-C*rw>ce- SubS'Vanc-e 4 A d n c l\. .+ t>7 - GfeO -c> GI3S7J Lab Request No. 6 t 3~/ Environmental Lab -- Work Sheet OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 Pafle------ ^-- of --~ -- Date: L -IS l Analyst: S x f A Stock and Test Solutions Preparation____________________ STOCK SOLUTION: ^ Q q Y*4 3.S~- 3>CP>j / o ZacJL. h o h d L ~ -ST m "*b r.s s .o iu g ^ ( f i \ S - b c ? m }O n J }3 l $ A J /^ d O M \u -k d ~k> Z o V)dL Uji+U J a i IIi' - G Lo^bfar- --"'A c lc h d , vwfer s > 4 im ig ^ i-O/j tfpSOy A r o f u i s z unit'I im 'ffc n t- f L lu~fgc/ 4 a ) 'Q L\A rec 6 0 j A i l l i ' - Q U ia^-P r--____________ fro c k - Z xplu'iioK . 7 ________________________ est Cone. mg/L SorEvJffoflTs ml stock soln per S00 ml -- i e _____ 10 V ml DI water ml syn. sewage ml microbial per 500 ml feed per 500 ml inoculum per 500 ml ?.?y h l & +- J o o 7 +- H a +- LO O ~ Total volume ml sro o S~o& ->rt4-tols> sJtnnteaK. cuhlA, Lft. 6 I3S*Q~) 001113 ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST RAW DATA #209 LR I. Test Substance: 1>j5 ~ 1o T o p h e v\o \ & g-C?rp^/<?_ S o K ^ n r * ___________ _ Source of Activated Sludge Mixed Liquor: M e h t n L u a ^ t e ^ T r 0 it b w r t - T >)an+- Date Received: _ 6> ________ Date Used: ^ A z fL z B S L ________ Analyst:, Cj & a d \ BEST COPY AVAILABLE 30 - MINUTE EXPOSURE 3 - HOUR EXPOSURE Test Substance ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS 3. 5 - 1 Reeferenfls 30 - MINUTE EXPOSURE Test Substance Concentration fme/L) ITSwriTlIfllHHHHIH ____________LQ__________ Oxygen Uptake Rate R 0 . .^ 0 ss~ O. 3 J4S R Corrected^ to 20 C G SCKSfT O. 3 o < W TD *)**'/* Date 6 *7-$*3 Analyst * Inhibition in > Respiration Rate'-*' -- V| V QQMTROLII________________ O' * 3 V S - _______ o S l G i T -- Percent Difference in Controls ^ 1, Q %> ECS0 (951 Confidence Limits) man. ! Q ihriuCei. H i 9o m h r k r t f c * Test Substance Concentration (mg/L) /0 Oxygen Uptake Rate R <? s u s r Q . lb 5 5 ~ R Corrected to V f C G - tT lln O .I rt % Inhibition in ^ Respiration RateU J - -- .. Ip S CONTROL II_____________ _ 0 . 5 5 S A _______ O ^ 3 0 * 1 Percent Difference in Controls ^ rc ^ ^ Q ^ rn n fid firirfi^ H r^ ^ > S C^>____________ (1) Temp, Corrections -- (mg 02/L-min.) IRC RCJ -- X 10 (2) \ Inhibition^** t ....l <* X 100 K v j4 The two control respiration-rates should be within 151 of each other Where: ^ c/ R - Oxygen uptake rate (mg 0 2/L-min.) . P A 4-^-fer S & \ * . r>r*r> i ^ i M T T x . tT & C. vfc C Control BEST COPY AVAILABLE ' 0 0 1 1 1 5_