Document by86yNmYGxkBeyVO0ayroeKdy

AR 86 _ 0824 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: A mixture containing referred to as PFOS, perfiuorooctanesuifonate; may also be FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: 206CF-X. CurTrheent3iMnfporromdautcitoinoinndloitcantuemsbietirs was 501. a mixture The test sample is FCof 0.5% PFOS, 10% d1i.e6t%hysloedneiugmlyoccotlylmosnulofbautte,yl1e%thseurl,f7on8a.t0e8d%alwkaytleert,he1r.3sa7l5ts%, a6c.ry0l%icufroeaa,me0r.,5% triethanolamine, and 0.05% tolyltriazole. cThhaerafcotlelroiwiznegd csounmcmeantrryataipopnlsieosftiompaurmiitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical component of the test sample. METHOD Method: GLP: No OECD Guideline 209 Year Completed: 1992 Analytical monitoring: temperature. Dissolved oxygen concentrations, pH, and Statistical inhibition in methods: respiration Rreatseu.lts were determined by calculation of the% TMeesttrooWragsatneiwsamtesrouTrrceea:tmAecnttivPaltaendt,sSltu.dPgaeulm,ixMeNd.liquor collected from the Test condition: DSiylnutthieotnicwasteewra:geMi:llippeorreOMEiCllDi-mQeTMtwhaotde.r. Rreeffeerreenncceesuabnsdtatnecset,s3o,l5u-tdiiocnhlporreoppahreanotli,onw:asApsrteopcakrseodlubtyiodnisosfotlhveing w5a0t0erm,gthienn1b0rmouLghItNtoNathOeH,poidnitluotfedintcoip3i0enmt LprweictiphitMaitliloinpowrietMhi1lNi-HQzTMSO4 - asonldutdiiolnutweadstome1 aLswuirtehdMtiollb-eQ"7TM.6w.ateTre."st TshubesptHanocfetwhaesreafdedreendcdeirectly to the test vessels. Test vessels: Not gIinviteinal. pH 6.8. Number of concentrations: one substance and 2 blank controls (duplicate) plus 1 reference Temperature: 18.6 21.6 C Total suspended solids and pH on day of testing: 2.6 g/L (TSS in - fEilnaelm: e1n.t04Bga/sLi)s,:iniRtieaslppirHat6i.o9nainndhifbiintailonpaHs7.d9e.termined by oxygen consumption. 001917 Remarks: Values were corrected to 20 C for calculations. RESULTS Nominal mglL concentrations: 2 Blank controls, reference substance and 1000 Element values: 30-minute EC50 = >1000 mg/L. 3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Substance Testing was conducted on Remarks field. The values the mixture as reported apply described in the Test to that mixture and not the fluorochemical proportion alone. = CONCLUSIONS `dTehteertemsitnseudbtsotabnec>e130-0h0oumrg/ELC.s,Tfhoer a1c0t0iv0atmedgslturdegaetmreesnptircaatuisonedin~hi1bi8t%ion was respiration inhibition at 3 hours. Submitter: 3M Company, Paul, Minnesota, 55133 Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY Reliability: However, Kiimisch ranking the sample purity 2. The was not study meets all criteria properly characterized for quality testing. and the study lacks analytical solution. confirmationofthe amount of fluorochemical proportion in the. REFERENCES This study Paul, MN, was Lab RceoqnudeuscttendumbybetrheK139M05C-o2m,pa19n9y2, Environmental Laboratory, St - OTHER Last changed: 6/28/00 001918 BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LGUOR oe Ton 200s Activated] Sludge Respiration Tnbubutien wDauisK411d3o6Js273. PAanagleyst oSlIEi #205 ACTIVATEDSLUDGE MIXEDLIQUOR oats Obtained 6/26/92. oats used 4/36/93 Mixed Liquor Suspended Solids MLSS)b.o on. Adjuste1d0 -- gn. MLSS in final test solution /- CY gr inital pH {> - Comments (1st test soln. prepared) Final pH Z.S (last soln. prepared) rv---- TEST sussTANGE Sample Description EC. 20LCF-X Lob 521 Commants Inital pH of highest conc. tested (JL oh - b.& Adjusted to pH _---- BEFERENCE SUBSTANCE sample Description _3,.5=Dic [lorepheno Indal pH of stock solute (00 mon) = Zola. Comments EXPOSURE CONDITIONS Test Duration __3- Hours, Test Tomporaturs Range: /8.L=2)b>. C Commis gAL LT nv uit Adjusted 1 pH _-- i Spain: i sLCoy AnENR Em pe I aRo N BHEe Ti EE$4LUN sTy egE y A A cigs Fb nfe A ee 003919 BEST COPY AVAILABLE owen. usowor FEES 56D ACTIVATTEeDsStLSUDiGmEoRnESsPmRATeINaRBnITONTET20 Tost substance: FC-J0lA odC SolE=X Sample Description: Amber Lug, dd Analyst: SAR oa: 4/26/92 . TestConc. CImea Lovie amlgadidend Daal miLMQ(!)water = ~~ ARY Ee EE Duplicate Soo ARY LRA mLsyn. sewage [bk 1b Wotan mLmicrobial 200 Im KoT> Total Volume "Epp sn S500 Iif 0 Wore RE M high A pu tr 1 5 ain ian $ ev a En TLeRiaE BEI Sent Raho ok a Sh bic CSHERIEAR Z LR SHEARGEE hOeea ps 1001920 BEST COPY AVAILABILE ceo AcTATEcDusmoowcETmAeLserLuimsaonmanToosnssmon Te eenl7ciesd ow oan Tot sobre: FC-20LCE-X Sourca of Activated Siudge Mixed Laver: MTeacr a(1e oncta e tmPe lann tS:Bt ol, mv' Date Receiveg: Lo JA | 9 Date Used: 2/24 [72 EEE [To TER a wo] TN--E--CTO SE Sm AT E E NRD A, ma oe Sem 0 Didizde om 2 [fo P.5 [3.[31.8 4[2.0| 1.3 3] Alo poppePlPERTE ILE 3LT BRAB14 M4 41 BI 59P.alaq [ TT TT TT 1 TT 1 T7 25. rT Delizleslsllste[imallss{asellss rT 3-HOUR EXPOSURE DISSOLVED OXYGEN CONCENTRATIONS, mol. TE e ermos| s TTEHREE, e ek llBTE 9B Prrpiate [6007 a 2A 0 16431 o.(o | (a2 al 120.8 2. plicate BL F3T 5[FF7[- RQ 1 FT116T dGtT [651 6,1 7[Ga1 lGI1A0 : = rr ir Tr pp 1 = f= seme alalealale izlr eoll ssle si v [saline H i7 oFosot.fi taomwpoaraiug ra: WAa ixod eebvor: CRTpnALTAaTgEeTNE mpio, a39g5n30 TE ee A Se ------__ ge 001921 | re | 3iLEL|IonEOoS euDwtiAonTmIR oAneTEmDsR LoLDuCSoRyESPRATINRHBTON TEST 38 nan ouui:: gSLo26s122 "ST COPY AVAILABLE ry | [CC EElSTeB re esgto 0erTpeiefpeReEeeeefoRe p eCeve{pCvaCwtNpCvaEstN{pCvaaoNe p poowr0| toaverpCooEwSneOTe a sopfjChooi1anwtles)] pL [E [ Earssrp o arfarpaanpop g wp pweepavete oae e s R aopR hneR pweeess e w jfeoea wsle]] Poise - fCuLea Tos CerTesTosTaTEoeksToropTetaaTmaeaaTorTaTso Tamm TnoymosT] a4 . REESE = fgopga[SLs[oapTroasLstooeTTooteeTotsTpTreeeTRorIvOaRlAee nttAorTe PwEnesfoRssoaTffsoea smal | S2> z SiSfpe mn:sp[ewmeaelph7e[pT7hv5ep[T7oeaa[pPThRvoeAaaTwRrpreeepeAsseTpTneoetrrpTTeoaRsseeppTTaaeoadtsToOAoaaEttaAorTrBonEsf[a OooMmmEe JofOomsNeIli=]] 3 3 (SEeSealcsetrrBrtee4s3torTeee[A okaoToenr7TCooRtsots o s aa| s oeTmomsRfaonmro | on A-- Em Jos verison . 88 ested BEST COPY AVAILABLE EEA, LABoRATORY aKi1g90s5 ORSD ACTHATTEDA$R00E0 SRESePEnRAnTNpNuBITONTEST 200 Tost suvsance: 3,5Dicblow phenal RelerenceSuhetance samote owscrpton: Ahh, Roodlobed LowD7oboe to son } Date: - Reparations S00 fx JC act 2pd =o 4 Dilsue tsSeeom]ip Senongaeald pL od) De ree ppl apuigepple pient PDE -- `TestCone. IT-_a--n_gi e 8KCanirol mLadded S-- mLMQ(!) water Z aa Mr IRED egreaen A84. mlsyn. sewage lb mlmicrobial 200 Total Volume Soo L TTT aa as ese ene T m Ee TraT e -- TT --T --a aes Tee e e ed = : ou Te - Fo ROREBOC7TARBA e RA TNN ROSEASTESCA OENTTD lel. 0). Minpri-dORHGHpiwait3y SX NR CE eT Shin Foti iii 007 2 ah Ss Tile aT CERN E pi aero TR RRR STR ee BEST COPY AVAILABLE OECD ACTIVATEENDVSILRUODMGEENRTEASLPIRLAATIBOONRARTHOIRIIYON TEST1209 sn Raw Daa TSoostcasuobfstAacntchea:tetT 3S.iu5o tgo Miadctiah uor: l MTedaer ac (Rese Go ieneca pe Sh ot lbe em tannce Peloann lSitu,iy DAantaelyRste:cH eive:mb/3paB/7a3 ch outa Uso: 6/26 [72 es . i a0uNuTE Exposure TSE DISSOLVED OXYGEN CONCENTRATIONS, mot. TRE VROTES a emm5a AuAeXlS 5zeMl13a3le3da acleolel 1d6e oaolleosls le[esttas S =m + 1 olelesle e5555a[51[4s [oa HOUR EXPOSURE DISSOLVED GevGEN ConoETATINS, mo 1. - CoTonsctoSnurbasttiaonncpe y; ) 20Jf12u5e4130nursa5|T4.500Ta [4s Tsoaa, |o5sT|Yeemes 4LSoEcomo RbIleOalRsO2B47T2wblAaIuG7a= hbesT Fziro enar isse fave HSioem Ee ee eee yeehe o e on o emon n a | e vse aan Be oi rtateeesswcioieldaield nBLsT o oB r e RE ea ee horeTiSs en SY SA BIE) ioei. 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