Document bkNvkg4G984Y4gbLoweaGa83

ARnt- ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity: e2rFPet9Cehf9er-yf11rllr-u2-eN5o7d1-r,[o-(tF7oho)Cecatp-as1tna2Ped9Fse,OucFlaSfCofAl-nu1Ayo0,lra9oFm,oCFcid-tC1yo-l2()1es80tuh9olyf-roXl)anasyoclrte]h-Rt,ea1ptem9o;0taa4mjso.asriy(ucGomalmlyscsopianolbetn,e,eCNnAt-Sof# Remarks: The test sample is FC-129 referred to by the test laboratory as R1904. Current information indicates it is a mixture of 42% test fsluuobrsotaanlkcyel,c3a2rb%oxwyalateter,c1o4m%po2u-bnudtso.xTyeetshtamnoalt,er4ia%l wetahsanfroolm, a3nMd 8p%rodCu4c-tCio7n lot 930014, mfg. date 3/97. DmtThhaeixetattufeomrselltaosoywufnibtnohsgtetaastnceuccsmeut.mrsauatbersylytiasrnealcabetbeirnetoavxiawictaeittdyebro/eofcrthgaeaunsteeicststhosealvtmeesnptltessaowmliutphtiloetnhi.astaof METHOD: TGMeLesPtht:toydNpsoe:: OStEaCticDa2c0u3te YASenpaearclyiCetiosc:malPpmilmeoteenpdiht:oa1rlei9ns9gp7:roTmemelpaesrature, pH, DO, and conductivity RESULTS Nominal concentrations: Bk control, 19, 32, 54, 90, 150, 250 mg/L (in dEulepmliceantet)values: 24974862----hhhhoooouuuurrrr 9966--hhoouurr LLLNLLCCCCCO55551E00000C===== 42>11542165(02(m21mg-28/g8L0-/L)1(C6m(7C.gl)../lLm.nongto/Ltcaclacluclualbalbel)e) 9=75(48m1-g1/1L6) mg/L All element values based on nominal concentrations. Remarks: Testing was conducted on the mixture of the test substance as tdoesthcaritbmedixtinurteheantedsnt ostutbhsetatnecset sruebmsatarknscefi.eldN.o Tahtteemvapltuwearsepmoartdeed taopplies 003S2- dpPeoertretfilorumnoirnooefotcthhteaentoeimxsipucaliftocytncoyalfantmhbeiedpocr(oeensthteryniblc)uaetceoedftatthoteet.hoerganic solvents or what CONCLUSIONS T97hemFgC/L-1w2i9th9a6-9h5o%urcLoCn5fi0dfeonrcPeimineteprhvaalleosfp8r1o-m11e6lasmwg/aLs. dTehteer9m6in-heodutronboe observed effect concentration was 54 mg/L. SPauublm, Mittinenr:es3oMtaC, o5m51p3a3ny, Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY tTRehseetliirnaegb,iislbitauyt:lalacKcklkimosfiasccnhhaalryraaticnctkaeilrniczgoa2nti.foirnTmeoasfttitiohnnegotmefsetetesststasmaulplblcesrt.iatenrciae fcoornqcueanltirtyations. REFERENCES Trehqisuesstut doyf twhaes3cMonCdoumctpeadnya.t AScI Corporation of Duluth, Minnesota at the OTHER Last changed: 5/17/00 O383 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 STUDY TITLE ACUTE TOXICITY OF R1904 TO FATHEAD MINNOW (Pimephales promelas) BATA STANDARD OECD GUIDELINE 203 STUDY COMPLETED June 27, 1997 TESTING FACILITY AScI Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY_JDENTIFICATION NUMBERS AScI Study ID# 5010-068 3M Company Lab Request# R1904 Sponsor: 3M Company Sponsor Study ID# R1904 1 000384 AScI Corporation/AScI-Duluth Environmental Testing Division AScl Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of R1904 to Fathead Minnow (Pimephales promelas). OECD Guideline 203. 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. Susan Beach, 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133; Tel No. (612) 778-7452. R1904. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Joe Dierkes Joe Amato Donald Mount June 17-21, 1997. Alan Mozol Report Signature^ Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Sponsor: 3M Company Sponsor Study ID# R1904 2 000385 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 2.0 OBJECTTVE(S) To determine the 96-hour 10% and 50% effect concentrations (LC10 and LC50), and the 96-hour no observable effect concentration (NOEC) of the test substance, R1904, for fathead minnow (PimephaJes promelas) under static test conditions. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 203 (OECD 1993). 3.0 TEST METHODS Test Substance Test Organisms I Dilution Water Properties'. R1904, bright-yellow liquid, specific gravity = 1.3 (water=1.0), stable, and completely soluble in water. Test concentrations: 0 (dilution water control), 19, 32, 54, 90, 150, and 250 mg/L. Test solutions prepared by mass addition of test substance to dilution water followed by vigorous stirring. Source: Healthy, juvenile fathead minnows (PimephaJes promelas) obtained from AScI-DETD in-house culture. Holding/acclimation: The fish used in the tests were obtained from AScI's in-house culture. The fish used in the study were hatched on May 24, 1997. For the 48 hours preceding test initiation mortality was 0%, and the fish were not fed for 24 hours before test initiation. The fish mean length was 17.0 2.6 mm, and mean wet weight was 38 23 mg. Loading rate was calculated to be 127 mg of fish per L test solution. Use in test: Used 10 organisms/replicate and 2 replicates/test concentration. Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, the water had a hardness of 48 mg/L (as CaCOj) and a pH of 8.03. Aerated for 24 h prior to use in the test. Sponsor: 3M Company Sponsor Study ID# RI904 3 000386 Exposure Chambers Incubation Observations Endpoint Calculations AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 Type: 4-L glass jars. Use in test: 3.0 L of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Solutions were not aerated during the exposures. Duration: 96 hours. Daily photoperiod: 16 h light and 8 h dark, maintained using cool white fluorescent lamps. Temperature: 21.0 1C. Biological: Daily- Test organisms with abnormal behavior and signs of stress, and dead test organisms. Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, DO, conductivity, and pH. All determinations were made according to APHA methods (1995). LC10: Inhibition Concentration (ICp) Method. LC5q s : Trimmed Spearman-Karber Method. NOEC: Fisher's Exact Test (TOXSTAT statistical software, Version 3.5, West Inc., and University of Wyoming). Sponsor: 3M Company Sponsor Study ID# R1904 4 000387 AScI Corporation^AScl-Duluth Environmental Testing Division AScI Study ID# 50104)68 3.0 RESULTS Test Substance Toxicity (Pertinent data are in Table 1) Ranges of Water Chemistry Parameters 24-h LC5q: >250 mg/L (Not Calculable). 48-h LC50: 211 mg/L (Not Calculable). 72-h LCS0: 146 mg/L (128-167). 96-hLCJ0: 45 mg/L (22-80). 96-h LC50: 97 mg/L (81-116). 96-hNOEC: 54 mg/L. Total hardness (as CaC03 mg/L): 46-48. Alkalinity (as CaC03 mg/L): 44-46. Temperature (C): 20.8 (No Range). DO (mg/L): 5.4-8.8. pH: 7.60-8.05. Specific conductivity (umhos/cm): 116-133. Sponsor: 3M Company Sponsor Study ID# R1904 5 000388 4.0 TEST QA/QC AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 QA/QC Criteria Data Mortality in control exposure must not be more 0% mortality. than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. The lowest DO concentration measured was 61% of the air saturation value at 21.0C. Test duration must be 96 h. The test duration was 96 h. Reference Substance Toxicity Test (this test is Control chart for sodium chloride reference optional). toxicity testing is in Raw Data Package. 5.0 REFERENCES American Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Organization for Economic Cooperation and Development (OECD). 1981/1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. Hamilton, M .A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. Technol. 11(7): 714-719; Correction 12 (4): 417 (1978). USEPA. 1993. A Linear Interpolation Method for Sublethal Toxicity: The Inhibition Concentration (ICp) Approach. Version 2.0. West Inc., and University of Wyoming. Toxstat Software Package. Version 3.5. Cheyenne, Wyoming. Sponsor: 3M Company Sponsor Study ID# R1904 6 000389 AScI Corporation/AScI-Duluth Environmental Testing Division AScl Study ID# 5010-068 TABLE 1. ACUTE TOXICITY O F R1904 TO FATHEAD MINNOWS (P. promelas) Teat Substance Concentration (mg/L) 0 (control) 19 32 54 90 I1 150 250 Percentages are in parenthesis. Cumulative Number o f Dead Organisms and Percent Mortality* 24 h 48 h 72 h 96 h 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 1(5) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 3(15) 0 (0) 0 (0) 2 ( 10) 8 (40) 0 (0) 0 (0) 9(45) 16 (80) 0 (0) 15 (75) 20 ( 100) 20 (100) I LETHAL AND NO-EFFECT CONCENTRATIONS W ITH CORRESPONDING 95% CONFIDENCE INTERVALS 1 LC 's and NOEC 24-h LC50 48-h LC50 72-h LC50 96-H LCjo 9 6 -h L C jo 96-h NOEC * Values based cm nominal test concentrations. Data (mg/L)" >250 211 146 45 97 54 95 % Confidence Interval Not calculable Not reliable 128-167 22-80 81-116 Not applicable Sponsor: 3M Company Sponsor Study ID# R1904 7 000390 ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity: Perfluorooctanesulfonylamido(ethyl)acetate; may also be erFetChfe-y1rlr-2eN7d-,[(tFohCeap-s1ta2Pd9Fe, OcFaSCfAl-u1Ao0,r9oF,oCFc-tC1y-l2)1s80u9olf-roXansyolrt]h-R,e1pm9o0taa4jso.sri(ucGomlmyscpainolten,,eCnNAt-Sof# 2991-51-7) fasRluuseobmRrsot1aaa9rnl0kkcs4ye:.l,cC3au2rTrb%rhoeexnwyttaleaitnsetfetro,srcma1om4am%tpipoleo2nu-ibsninudFdtsoiCc.xa-Ty1teee2tss9htairtmenisofaelta,rerr4emia%dilxtwoteutarhbseaynfotrhooflem4, 2tae%3nsMdttel8pas%rbtoodCrua4ct-otCiroy7n lot 930014, mfg. Date 3/97. DtmThhaeixetattufeomrselltaosoywufnbitnohsgtetaastnceuccsmeut.mrsauatbersylytiasrneaclabetbeirnetovaxiawictaeittdyebro/eofcrthgaeaunsteeicststhosealvtmeespntltessaowmliutphtiloethnia.staof METHOD: AYTGMSeenpLesaeaPthtrcl:ytoiCyetdNipsocso:ema::DlpOmSaleptEoatheCtnindciDita:oa2r1cmi0u9na29tgeg7:nTaemperature, pH, DO, and conductivity RESULTS Nominal concentrations: Bk control, 1.4, 2.3, 3.9, 6.5,10.8,18, 30, 50 mg/L (in duplicate) Element values: 4284--hhoouurr EECC-5ioo== 4>.540(m4.3g/-L 4(C.6.l). mnogt/Lcalculable) 4488--hhoouurr ENCO5E0C= =9.13.9(7m.4g-/1L1) mg/L All element values based on nominal concentrations. tddRoeeettsmhecarramitbrmikensdeix: titTnuhreetehsiteaminntpgedaswcnt taosostutfbchtsohetneatdenpuscrcteetsserueedbnmoscatnearnktohscfeetfhi.meeldNixo.otrugTaraehttneeoicmfvtaspholtuelwveteearnssettpsmsoouartrbdeswedthataoanptcpelieass 000391 pPoerrtfilounoroofotchteantoexsiucliftoyncyalanmbiedoc(oenthtryibl)uatceedtatotet.he CONCLUSIONS oTmbhgse/eLFrvwCei-tdh12ea9ff9e45c8%t-hcooconuncrefEindCter5naoctifeoonrinDwteaarpsvha3nl.i9oafmm7g.a4/gL-n.1a1wmasg/dLe. teTrhmein4e8d-htooubren9o.1 SPauublm, Mittinenr:es3oMtaC, o5m51p3a3ny, Environmental Laboratory, P.O. Box 33331, St. DATA QUALITY TtRehesetliirnaegb,iislbitauyt:lalacKcklkimosfiasccnhhaalryraatcinctkaeilrniczgoa2nti.foirnTmeoasfttitoihnnegotmefsetetesststasmaulplblcesrt.iatenrciae fcoornqcueanltitryations. REFERENCES Trehqisuesstut doyf twhaes3cMonCdoumctpeadnya.t AScI Corporation of Duluth, Minnesota at the OTHER Last changed: 5/17/00 000392 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 STUDY TITLE ACUTE TOXICITY OF R1904 TO DAPHNIA MAGNA DATA STANDARD OECD GUIDELINE 202 STUDY COMPLETED June 17, 1997 TESTING FACILITY AScI Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION NUMBERS AScI Study ID# 5010-068 3M Company Lab Request# R1904 Sponsor: 3M Company Sponsor Study ID# R1904 1 000393 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of R1904 to Daphnia magna. OECD Guideline 202. 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. Susan A. Beach, 3M Environmental Technology and Safety Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. Tel No. (612) 778-7452. 8 R1904. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Holly Kitterman. Joe Amato. Donald Mount. June 10-12, 1997. Report Signature* Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Sponsor: 3M Company Sponsor Study ID# R1904 2 000394 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 2.0 OBJECTIVE(S) To determine the 48-h EC10 and EC50, and 48-h NOEC (no observed effect concentration) of the test substance, R1904, for Daphnia magna. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 202 (OECD 1993). 3.0 TEST METHODS Test Substance Test Organisms Dilution Water Exposure Chambers Properties: R1904, yellow-colored liquid, specific gravity Ca. 1.3 (water = 1), stable, and completely soluble in water. Test concentrations: 0 (dilution water control), 1.4, 2.3, 3.9, 6.5, 10.8, 18, 30, and 50 mg/L. Stock solution prepared by diluting 200 mg of test substance in 1 L of dilution water. Test substance concentrations were prepared by direct addition of stock solution into dilution water. A 0.6 dilution series was utilized. Source: Healthy, less than 24-h old Daphnia magna neonates obtained from AScI stock cultures maintained in water of test dilution water quality. Use in test: Gravid adults isolated in dilution water 24 h before the test initiation. Used 5 organisms/replicate and 4 replicates/test concentration. Handled with a smooth bore pipet with an inside diameter of 6 mm. Did not feed during the test. Source: Dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis is in Raw Data Package. Characteristics: At test time, the water had a hardness of 46 mg/L (as CaC03) and a pH of 7.93. Aerated for 24 h prior to using in the test. Type: 250-ml borosilicate glass beakers. Use in test: 200 ml of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Sponsor: 3M Company Sponsor Study ID# R1904 3 000395 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 Incubation Observations Endpoint Calculations Duration: 48 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white fluorescent bulbs. Temperature: 21 C. Biological: Daily- gently agitated the exposure chambers and then counted immobilized and dead test organisms (immobilized organisms were those that did not swim within 15 seconds after exposure chambers were gently agitated). Did not remove any daphnids from the exposure chambers at 24 h. Water chemistry. (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, pH, DO, and conductivity. All determinations were made according to APHA methods (1995). ECS0: Trimmed Spearman-Karber method. EC10: Inhibition Concentration (10%) USEPA software package for Determining ICp concentrations. NOEC: Fisher's Test (TOXSTAT statistical software, Version 3.5, West, Inc., and University of Wyoming). 4.0 RESULTS Test Substance Toxicity (Pertinent data are in Table 1) 24-h EC$q`. > 50 mg/L. 48-h EC5q: 9.1 mg/L (7.4-11.0). 48-h EC10: 4.4 mg/L (4.3-4.6). 48-h NOEC 3.9 mg/L. Sponsor: 3M Company Sponsor Study ID# R1904 4 000396 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 Ranges of Water Chemistry Parameters Total hardness (as CaC03 mgfL): 44-46. Alkalinity (as CaC03 mg/L): 42-44. Temperature (C): 20.5-20.8C. DO (mg/L): 8.1-8.5. pH: 7.81-8.04. Specific conductivity (umhos/cm): 118-126. 5.0 TEST QA/QC QA/QC Criteria Test organism dead, immobilized or trapped in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. Test duration must be 48 h. Reference substance toxicity test (this test is optional). Data 0% dead, immobilized or trapped. The lowest DO concentration measured was 91% of the air saturation value at 21.0C. The test duration was 48 h. Current control chart for D. magna acute testing is included in the raw data package. Sponsor. 3M Company Sponsor Study ID# R1904 5 000397 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 5.0 REFERENCES American Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Hamilton, M .A., R.C. Russo, and R.V. Thurston. 1977. Trimmed Spearman-Karber Method for Estimating Median Lethal Concentrations in Toxicity Bioassays. Environ. Sci. Technol. 11(7):714-719; Correction 12(4):417 (1978). Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for 0 Testing of Chemicals. OECD Publication Information Center, Washington, D.C. West Inc., & University of Wyoming. TOXSTAT Software Package Version 3.5. US EPA. 1993. A Linear Interpolation Method for Sublethal Toxicity: The Inhibition Concentration (ICp) Approach (Version 2.0). Environmental Research Laboratory, Duluth, Minnesota. Sponsor: 3M Company Sponsor Study ID# R1904 6 000398 AScI Corporation/AScI-Duluth Environmental Testing Division AScI Study ID# 5010-068 TABLE 1. ACUTE TOXICITY OF R1904 TO DAPHNIA MAGNA Test Substance Concentration (mg/L) 0 1.4 2.3 3.9 6.5 10.8 18 30 50 Cumulative Number and Percent o f Dead and Immobilized Organisms* 24 h 48 b 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 0 (0) 10 (50) 1(5) 17 (85) 1(5) 0 (0) 4(20) 14 (70) 16 (80) 20 (100) * Percentages are in parenthesis. EFFECT AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS C j0 and NEC 24-h ECS0 48-h ECjq 48-fa EC10 48-h NOEC Data (mg/L) >50 9.1 4.4 3.9 95% Confidence Interval Not Calculable 7.4-11.0 4.3-4.6 Not Applicable Sponsor: 3M Company Sponsor Study ID# R1904 7 000099