Document baKn03Logp65eMaypNzK589Z1

AR32-33? ACUTE TOXICITY TO AQUATIC INVERTEBRATES - Daphnia magna TEST SUBSTANCE_______________________________________________ Identity: N-Ethylperfluorooctanesulfonamide; may also be referred to as U1464, EtFOSA, EtPOSA, F-6309, or FX-12. (1Octanesulfonamide, 1,1,2,2,3.3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-N-ethyl-, CAS # 4151-50-2) Remarks: Material is a white solid. Sample purity and lot number were not recorded. METHOD Method: Adapted from OECD 202 - Water accommodated fractions Type: Static acute GLP: No Year completed: 1998 Species: Daphnia magna Analytical monitoring: Nominal concentrations only. Temperature, pH and dissolved oxygen recorded Statistical methods:: EL50 (lethal loading) values were calculated using the probit method. The NOEL was calculated by Fisher's Exact Test, TOXSTAT Software Package, Version 3.5, Laramie, Wyoming. Test daphnid source:. ASci in-house culture, Duluth, MN Test daphnid age at study initiation: < 24-hour old neonates Test conditions: Dilution water: Carbon-treated dechlorinated City of Duluth, MN tap water aerated for 24-hours prior to use in the test. Dilution water chemistry during the study: Hardness 44 mg/L as CaC03 DO: 8.1-8.9 mg/L Alkalinity: 42 mg/L as C aC 03 pH: 7.9-8.2 Conductivity 113-125 pmhos/cm Test Temp. Range: 21.3-21.5 Stock and test solution preparation: Test solutions prepared by mass addition of test substance to 2.0L aliquots of dilution water (one per concentration). The solutions were vigorously stirred (vortex 1/3 to J4 solution depth) for 18.5 hours. The aqueous phase was siphoned from the vessel at mid-depth. Exposure vessels: 250 mL borosilicate glass beakers 006049 Exposure volume: 200 mL Number of replicates: four Number of daphnids per replicate: 5 Number of concentrations: Five plus a negative control Element basis: mortality RESULTS______________________________________________________ Nominalloading concentrations: Bk control, 5.8, 9.6 ,16 , 27, and 45 mg/L Element value: 24-hour EL50: 24.6 (21.3-28.6) mg/L 48-hour EL50: 14.5 (12.1-17.4) mg/L 48-hour ELi0: 7.3 (5.0-9.2) mg/L 48-hour NOEL: 5.8 mg/L Mortality of controls: 0% after 48-hours exposure Percent Mortality Nominal Loading Concentration, mg/L 24-Hours 48-Hours Negative Control 5.8 9.6 16 27 45 0 0 0 15 50 100 0 0 35 50 85 100 Remarks: All values reported are for nominal loading concentrations. No measurement of test concentrations was conducted. CONCLUSIONS The test sample 48-hour EL50for Daphnia magna was determined to be 14.5 mg/L with a 95% confidence interval of 12.1 - 17.4 mg/L Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking 2. This study, while well conducted using the methodology acceptable at the time, lacks analytical data for determination of the test substance concentration in the test solutions and determination of the sample purity. Testing was also done well above the 006050 solubility limit of the test substance with the use of water accommodated fractions. REFERENCES__________________________________________________ Study conducted at the request of 3M Company by, AScI Corporation, Duluth, MN. O T H E R ________________________________________________________ Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/18/00 AScl Corporation/AScl-Dululh Environmental Testing Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 STUDY TITLE ACUTE TOXICITY OF U1464 TO DAPHNIA MAGNA DATA STANDARD OECD GUIDELINE 202 STUDY COMPLETED May 20, 1998 TESTING FACILITY AScl Corporation/AScI-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811-5712 Tel. No. (218) 722-4040 Fax No. (218) 722-2592 STUDY IDENTIFICATION'NUMBERS AScl Study ID# 5010-068 3M Company Lab Request# U1464 Sponsor: 3M Company Sponsor Study ID# U 1464 1 006052 AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID# 5010-068 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Request# Testing Facility Principal Investigator Project Manager Testing Facility Director Definitive Test Dates Data Validation and Report Review Acute Toxicity of U1464 to Daphnia magna. OECD Guideline 202. 3M Environmental Technology and Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. Rochelle R. Robideau, 3M Environmental Technology and Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. Tel No. (612) 778-7065. U1464. AScI Coiporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Todd McGuire. Joe Amato. Donald Mount. April 28-30, 1998. Alan Mozol. Report Signature:.* Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Sponsor: 3M Company Sponsor Study ID# U1464 2 006053 AScI Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID# 5010-068 2.0 OBJECTIVE(S) To determine the 48-h EL's (effective loading's) EL10 and EL50, and 48-h NOEL (no observed effect loading) of the test substance, U1464, for Daphnia magna. This study was conducted according to the AScI study plan retained by the Sponsor and OECD Guideline 202 (OECD 1993). Test solution preparation was water-accommodated fraction (WAF) per Girling & Whale 1994. 3.0 TEST METHODS Test Substance Test Organisms Dilution Water Exposure Chambers Properties: U1464, white crystals, specific gravity 1.7 (water = 1), stable, and negligible solubility in water. Test loadings: 0 (dilution water control), 5.8, 9.6, 16, 27, and 45 mg/L. Each test solution prepared by mass addition of test substance to 2.0-L aliquots of dilution water. Each solution was vigorously stirred (vortex 1/3 to 1/2 of flasks' depth) for 18.5 hours to obtain WAF's. Test solution obtained from flasks by siphoning aqueous phase from mid-depth. Source: Healthy, <_ 24-h old Daphnia magna neonates obtained from AScI mass organism cultures maintained in dechlorinated City of Duluth tap water. Use in test: Gravid adults isolated 24 h before test initiation. Used 5 organisms/replicate and 4 replicates/test loading. Did not feed during the test. Source: Carbon-treated dechlorinated City of Duluth, MN, tap water. The most recent chemical analysis of post carbon (PC) water is in the Raw Data Package. Characteristics: At test time, the water had a hardness of 44 mg/L (as CaC03) and a pH of 7.99. Aerated for 24 h prior to using in the test. Type: 250-ml borosilicate glass beakers. Use in test: 200 ml of dilution water or test solution/chamber. During the test, kept covered, except when experimental observations were made. Sponsor: 3M Company Sponsor Study ID# U1464 3 v/ v# x : AScI Corporation/AScl-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScI Study ID# 5010-068 Incubation Observations Endpoint Calculations Duration: 48 h. Daily photoperiod: 16 h light and 8 h dark, maintained using cool-white fluorescent bulbs. Temperature'. 21 C. Biological: Daily- counted dead test organisms. Did not remove any daphnids from the exposure chambers at 24 h. Water chemistry: (1) At test initiation- total hardness and alkalinity, (2) daily- temperature, pH, DO, and conductivity. All determinations were made according to APHA methods (1995). EL10 and EL50: EPA Probit Method. NOEL: Fisher's Exact Test (TOXSTAT statistical software, Version 3.5, University of Wyoming & West Inc.). 3.0 RESULTS Test Substance Toxicity (95% Confidence Interval) Pertinent Data are in Table 1 Ranges of Water Chemistry Parameters 24-h ELS0: 24.6 mg/L (21.3-28.6 mg/L). 48-h ELS0: 14.5 mg/L (12.1-17.4 mg/L). 48-h ELl0: 7.3 mg/L (5.0-9.2 mg/L). ' 48-h NOEL: 5.8 mg/L. Total hardness (as CaC03 mg/L): 44. Alkalinity (as CaC03 mg/L): 42. Temperature (C): 21.3-21.5C. DO (mg/L): 8.1-8.9. pH: 7.9-8.2. Specific conductivity (umhos/cm): 113-125. Sponsor: 3M Company 0 0 6 0 5 5Sponsor Study ID# U1464 4 AScI Corporation/AScl-Dululh Environmental Testing Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 4.0 TEST QA/QC QA/QC Criteria Test organism dead, immobilized or trapped in control exposure must not be more than 10%. The lowest DO concentration at test termination must be at least 60% of the air saturation value at test temperature. Test duration must be 48 h. Reference substance toxicity test (this test is optional). Data 0% dead, immobilized or trapped. The lowest DO concentration measured was 91% of the air saturation value at 21.0C. The test duration was 48 h. Current control chart for D. magna acute testing is included in the raw data package. 5.0 REFERENCES American Public Health Association (APHA). 1995. Standard Methods for the Examination of Water and Wastewater. APHA, Washington, D.C. Girling, A.E., G.F. Whale, and D.M.M. Adema. 1994. A Guideline Supplement for Determining the Aquatic Toxicity of Poorly Water-Soluble Complex Mixtures using WaterAccommodated Fractions. Chemosphere, Vol. 29, No. 12, pp. 2645-2649. Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. University of Wyoming & West Inc. 1996. Toxstat Version 3.5. Cheyenne, Wyoming 82001. USEPA Probit Analysis Program. Version 1.4. 006056 Sponsor: 3M Company Sponsor Study ID# U1464 5 BISHOP AScl Corporation/AScI-Duluth Environmental Testing Division Submitted to 3M Co. - 5/98 AScl Study ID# 5010-068 TABLE 1. ACUTE TOXICITY OF U1464 TO DAPHNIA MAGNA Test Substance Leading (mg/L) 0 5.8 9.6 16 27 45 Cumulative Number and Percent o f Dead Organisms* 48 h 0(0) 0(0) 0(0) 3 (15) 10 (50) 20 (100) 0(0) 0(0) 7(35) 10 (50) 17 (85) 20 (100) * Percentages are in parenthesis. EFFECT AND NO-EFFECT LOADINGS WITH CORRESPONDING 95% CONFIDENCE INTERVALS EL's and NOEL 24-h EL50 48-h EL50 48-h EL10 48-h NOEL Data (mg/L) 24.6 14.5 7.3 5.8 95% Confidence Interval 21.3-28.6 12.1-17.4 5.0-9.2 Not Applicable Sponsor: 3M Company 006057 Sponsor Study ID# U1464 6