Document ba81x59Rd0b4Kr6KEQDDYjn0D

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredto as PFOS, FC-95, or as a component of FC-600. (1-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium saltC,AS # 2795-39-3) Remarks: The 3M productionlotnumber was notnoted. The test sample isFC-600 (Belgium).Currentinformatioinndicatesitisa mixture of 1.0% PFOS, 81.20% water,12.00% diethyleneglycolbutylether, 1.00% sodium octylsulfate2,.00% propane Sultonefoamer, 1.00% sodium decylsulfate0,.85% xanthan gum, 0.1% N-(3-chloroallyl) hexaminium chloride0,.80% starch,and 0.05 % tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentration of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the test sample. METHOD: Method: Not noted. Test type:Acute stati(cRange-flnding) GLP: No Year Completed: 1983 Species: Pimephalespromelas Supplier:Commercial supplierinBrady,Nebraska Analyticalmonitoring:Temperature,pH, DO Exposure period:96-hours Statisticamlethods: NotapplicableN.o mortalitaythighest concentrationtested. Test fishage: Juvenile Average Length and weight: Length = 3.6cm Weight = 0.39 grams Loading: 0.65 g/L Pretreatment: Not noted. Test conditions: Dilutionwater: Carbon-filterewdellwater,St.Paul,MN. Dilutionwater chemistry: pH: 8.2 Dissolved Oxygen: 9.6 mg/L Temperature range: 180C Lighting: Not noted Stockand testsolutiopnreparationT:estsolutiownesrecreatebdy directweights additions. Concentrations dosing rate:Once Stabilityof the testchemical solution:Not noted Exposure vessels: Wide-mouth one gallonglassjarscontaining3 liters of testsolution. Number of replicates:One Number of fishper replicate:5 Number of concentrations: Four plustwo blankcontrols Water chemistry during the study: pH range (0- 96 hours): 7.9- 8.2 (controlexposures) 7.7- 8.2(1,000mg/L exposure) Dissolved Oxygen range (0- 96 hours): 5.8- 9.6mg/L (controelxposures) 1.5- 9.6mg/L (1,000mg/L exposure) Temperature range (0- 96 hours): 18 - 210C RESULT Nominal concentrations: 2 blankcontrols,1,10, 100,and 1,000 mg/L Element value: 96-hour LC50 = >1,000 mg/L Element value based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuereportedappliestothatmixtureand notthe fluorochemicalproportionalone. CONCLUSIONS The FC-600 96-hour LC5o forPimephales promelas was determined tobe >1,000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. The studymeets allthe criterifaorquality testing.However, the sample puritywas not propedy charactedzedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemical proportioninthe solutions. REFERENCES This studywas conducted by 3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number 8875, 1983. OTHER Last changed: 6/28/00 ENVIRONMENTAL LABORATORY AOUATIC 'MXICITY DATA SHEET EXPLORATOR@ (RANGE-FINDIAG TEST) LR Type Tesu Material Tested &7-4//&J,&dlLot Test Organism (4fIprvg.)$Ourc 0 D ate Recld/6/L:6./i@atch No.z--8:5 Avg. Wt. eal grams Avg. Sizt_n@9--CM No. Organisms per dose 5 Test Solution Volume 3 Liters/All-Glass Tank Tank Dimns ions:W IDE -MOUTH I GALLON GLASS,JAR Time Started-//,.Io Date Started //o 7 '/ Dilution Water Carbon-filtered.well water_(Temp. Date Completed OC.. D.O. 'ItIlo t ----45 una- pH.5&) Investigatorjg T@- (FullSignaiu-re)- LCS(O9s% conflaeinicieait>s)IC)QQU4Q I.I@ Init. nk Conc. L---W I Total Amount I Added 24 urs 48 Total D Total No. Dead IpH pps0-i1 No. Dead urs 72 Ho 96 Ho PH ID.O. Tot 1 tal 0. ppo I No.::adLH F"0. @moo. De adT H @ --mP Control i@2 EL -Al gU A lpA 1 6 a--In 10c) Test Tom. OC. I Dato Co@nts:* I ki Leif Effectsother than Mortality ECSO Tan*k No. - amptons or Reactions controllr L 24-Hr. -AIAdZC. Total No. Affoctod 48 Hr. 72-Hr. I- 96-Hr.