Document ba6yxmObO34oZEEKK1ByO4Dw0

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95,oras a component ofFC-203. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notnoted.The testsample isFC-203. Currentinformatioinndicateistisa mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol,2.66 % Sultonefoamer,3% sodium octylsulfate0,.1% sodium laurylsulfatea,nd 0.05% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizedconcentrationsof impurities.Data may not accurately reflecttoxicityof the tluorochemicalcomponent of the testsample. METHOD: Method: Standard Methods (APHA), 1970 Edition Type: Acute static GLP: No Year completed: 1973 Species: Daphniamagna Supplier:In-houseculturemsaintainedatBionomics,Inc.W,areham, MA.. Analyticalmonitoring:Temperature,pH,conductiviatnyd DO Exposure period:48-hours Test organism age: 12 12 hrs.old Statisticamlethod: TL50(mediantolerancleimitv)aluescalculateudsinga linearegressionequation. Test conditions: Dilutionwater: Well water Dilutionwater chemistry: Alkalinity: 35 mg/L as CaC03 pH: 7.1 Lighting: Not given Stock and test solution preparation: Exposure concentrations prepared by directadditionoftestsubstance. Exposure vessels: 250 mL beakerswith200 mL exposure solution Number of replicates:4 Number of daphnids per replicate:5 Number of concentrations:fiveplusa blankcontrol Food: Food, consistingofa homogenizationof startetrroutfood and Cerophylinwater,was added atrateof0.5mUL afterintroductionftest substance. Water chemistry during the study: pH range (0-48hours): 7.1 Temperaturreange(0-4h8ours):21 t 1OC Dissolveodxygenrange(0-4h8ours):5.3- 8.9mg/L RESULTS NominalconcentratioBnks:contro5l6,0,870,10001,800,and3200mg/L. Elementvalues: 48-houErC50 = 1560(1280-2140)mg/L Element valuesbased on nominalconcentrations Remarks: Testingwas conductedon the mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportioanlone. CONCLUSIONS The testsubstance48-hourEC50was determinedto be 1560 mg/L witha 95% confidenceIntervaolf 1280 to2140 mg/L. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.Thisstudymeets the criterifaorquality testing.However,the sample puritywas notpropedycharacterizeadnd the studylacksanalyticaclonfirmatioonftheamount offluorochemicaplroportion inthe solution. REFERENCES Testwas conductedby Bionomics,Inc.o,fWareham, MA atthe requestof the3M Company, St.Paul,MN, 1973. OTHER Last changed: 6/27/00. BIOASSAY REPORT SUBMITTED-TO MINNESOTA MINING & MANUFACTURING ST. PAUL, MINNESOTA COMPANY ACUTE TOXICITY OF 3M SA@IPLES (A AND B) TO THE WATER (Daphnia magna)AND SCUD (Gammarus fasciatus FLEA. Bionomics, Inc. 790 Itain Street Warefiam, Massachusetts December, 1973 METHODS AND tiATERIALS These investigations were performed at the aquatic invertebrate toxicology laboratory of Bionomics, Inc., Wareham, Massachusetts. The susceptibility of the water flea, Daphnia magna and scud Gammarus fasciatus to 3M samples designated as sample numbers A and B (100% active) was evaluated under static conditions for a 48 houi: experimental period. Result-s were,expressed as the median tolerance lim b- nromcinuasl concentration of the test c@om;o'@rTiL@@@0wbate a ing 50 percent mortality. The TL50 values and 95% confidence intervals were calculated by converting the test concentration and the corresponding observed percent mortalities to logs and probits, respiectively.. These values were then used to calculate a linear,regression equation. Both Daphnia and Gammarus were obtained from laboratory cultures and judged to be in excellent con'dition. Procedures for the static bioassays adhered to the protocol described by the 1970 edition of Standard Methods (APHA). Static Daphnia and Gammarus bioassays were conducted 'in 250 ml beakers with 200 ml of the experimental diluent. The diluent water was obtained from a well source and was used as.such. The experimental chambers were maintained in a water bath at 210 + 1C. Five 12 12 hour old Daphnia were introduced into each experimental beaker and five fourth instar Gammarids were introduced int6.ea,ch experimental chamber for the Daphnia and Gammarus bioassays, respectively. In the Daphnia bioassays, food was added at a rate of 0. 5-ml/liter.after the introduction of the compound to the test vessels. Bioassays were conducted using four.replicates of each concentration for both compounds (A and B) for both species. The ph.of the standard diluent was 7.1 and the methyl orange alkalinity was 35 ppm as CACO 3* Dissolved oxygen values for the various test vessels for both compounds ranged from 8.9 initially to 5.3 mg/l at the*end of the test. 1Food was prepared by homogenizing 5'g of starter trout food and 1 g of Cero-phyl in 100 ml of water. RL-:SULTS The predicted 48 hour TL50 (i.e. that.concentrationof active compound that allows 50 percent survival to the experim*ental animals) and 95% confidence intervals for Gammarus and Daphnia exposed to compounds. A and B are presented in Table 1. A summary of the observed mortality of . Daphnia and Gaimmarus exposed to various.concentratibns of compound A is given in Table 2. The observed mortality of Daphnia and Gammdrus exposed-to experimental concentrations of compound B is given in Table 3.- SUBMITTED BY: PREPARED BY: APPROVED BY: Bionomics, Inc. 790 Main Street Wareham,.Mass. December, 1973 S. Krogh Derr, Ph.D. Invertebrate Toxicologist, Kenneth J. Macek, Ph.D. Director /db Table 1 Acute toxicity of 3 M compounds A and B to the water a flea (Daphnia magna) and scudb (Gaumarus fasciatus) exposed for a 48 hour experimental period. Species 48 hour TL 50-mg/ liter Compound A Compound B -kirpti,t-a' Daphnia-magna 31.1 (23.3 41.5)c 1560.0 (1280.0-2140.0). Gammarus fasciatus 15.4 (12.8-20.1) 1130.0 (843.0-1310.0) a Bioassay conducted at 21o + 1c, Daphnia, 12 12 hours old at initiation of tests. b Bioassays conducted at 210C 1C,'Gammarus, fourth ifi.starat initiation of tegts. c95% confidence intervals.. Table 2 Concentrations tested and corresponding observed percent mortalities for-the.water flea, (Daphnia magna),- and such (Gammarus fasciatus) exposed to 3M compound A for a 48 hour experimental period. Species Concentration mg/l Water Flea (Daphnia magna) 42.0 .32.0 @4. 0 12'.0 10.0 7.5 Control Mortality Observed .48 hour 100 60 0 0 0 0 0 Scud 32.0 100 (Gammarus fasciatus) 24.0 70 18.0 60 12.0 50 7.5 0 Control 0 Table 3 Concentrations tested and corresponding observed percent mortalities for the water flea (Daphnia magna) and scud'(Gammarus fasciatus) exposed to 3M compound B for a.48 hour experimental period. Species Water flea (Daphnia magna) Concentration mg/l 3200.0 1800.0. 1000.0 870.0 560'.0 Control % Mortality Observed 48 hour 100 45 5 0 0 0 Scud (Gammarus fasciatus) 3200.0 1800 .0 1000.0 870.0 560..0 Control 100 90, 4@@ 40 0 0