Document ba4j2v9k4dJ6epndnXj6v5Lq3

BIOACCUMULATION (ANALYTICAL ONLY) TT ESTe SUBSg TANT CE su=e = 0s 000T 00AN 00G 00 E IadsenPtFitOy:A ammPoenrifluumorsoaoltc,taAnmoimcoanciidu,mapmemroflnuicuromocstaaltn;camtae,y FaCls-o11b6e,rFeCfe-r1r2e6d,to 3F8C2-51.6296,-1or) FC-143. (Octanoic acid, pentadecafluoro-, ammonium salt, CAS # Remarks: The 3M production lot number was 37. The test sample is FC-143. It's purity was not sufficiently characterized, tshuobusgthanccuerraenndt i0n-fo3rm.a5t%ioCn,inCdri,caatneds iCt sispaermfilxuctruoreaonfal9o6g.u5e- c1o0m0p%outnesdts. Me MRErTNHeOD - e Method: ~~ Dbieovaicsceudmbuyla3tMe fCiouomrpoachneymitcoadlestferrommin3eM'ifsblDueecgialtlurs,unAfiLshplant GLP (YN): effluent No (See Remarks) Year completed: 1978 Remarks: The following method was devised and used: 1. 2. TOwnoelolottswoafs3e0xfpioshseedactho wDeecraetuursepdl.ant effluent from Oct. 5 to Oct. 25. TtohOecto.th2e5r.lot was exposed to Tennessee River water only from Oct. 3 3. tTewset)lvaendfitshhefrroemmatihneinefgfleuiegnhtteeexnpofsisehdwleort ewesracericfoilcleedctiendDdeecaatdur(.dieTdhien fiver were wsaatcerirfiecxepdoisneDdecfiasthurs.urvived through the exposure period and all 4. sEhfiflpupeendt-inexdproyseicdeatnodthreiv3erMwaEtnevri-reoxnmpeonsteadlfiLsahbworearteoralyl ipnreSts.erPavueld,aMndN 5. fFoirshanwaelyrseish.omogenized using a Tekmar Model SDT tissuemizer. 6. aTcweota2tge.anpodrttioolnusenweeroen wteheigohtheedr.and extracted. One portion using ethyl 7. Samples were analyzed by GC, TLC, and GC/MS. BREESUTLSTS eee -- Analytical Observations: Twehiegehfefdlu3e3n.t0-egxrpaomsse.d fish weighed 25.7 grams. The river water-exposed fish 03667 GC/MS effluent analysis of ethyl acetate contained no detectable aamnodutnotlsuoenfeaemxmtroanctieudmfipsehreflxupoorsoeocdtatnocpaltaen.t No fluorochermicals were detected in the river water-exposed fish. mRaenmuafrakcst:uresSftluudoyrowcahesmiccoanldsu.ctWeadtebrecuasuesdein3Mm'asnuDfeaccattuurrinpglainstlater treated and the treated effluent discharges eventually into the Tennessee River. c0 CoOnNcClLu0 UsSiIOoNn0 Ss 0000000 0 No reliable conclusions can be derived from this study. Submitter: 3M Paul, Minnesota, Company, 55133. Environmental Laboratory, P.O. Box 33331, St. b0 DaAtTaAgQuUaAvLImTlYY0 . 000 fRaecliilaitbyilriattyh:erKtihmainsacth3rWa'nskiEnngv3i.roSntmeundtyawlaLsabcoornadtuorcyt.edEfatfltuheentmaconnucfeancttruartiinogns of subject exposure fluorochemicals were not of the fish was not found. cThhaerraectweariszeadlsaonndotihnefsopremcaitfiiocnpornotocol for Nanianlyesfifsluoefntthies TkennonwenstsoeceaRuisveerdewaattherduorrienfgflNuPenDtEuSsetdesitnintgh,eosrtuldoyw (DeO.g,.,hNigHhs- TDS). of the Additionally, itis not fish prior to sacrifice. kPnroewsennicf etohfertehweatsesatnsyubospptoarntcuenictoyulfdor depuration have been caonncoernmtarlatfiuonncst.ionNoof etxhpelfainsahticoonnciesnattrtateimopntsedhoamsotgoewnhiazitnwgawsitthhethceaeufsfelouefntthe w1h2edtehaedr tfihsehsiunbtjheectefcfhleumeincta-lexwpaossaecdtugraolulpy.acNcoumduilffaetreedntiiantitohnewfiassh ogrivseinmptloy sorbed on the described. surface of the fish body. Sampling locations were not fRcEFERENCE=S V3rMasTpeicrh/niArctahlurReMpeonrdtel"A,naPlryojseicstfo9r97Fl0u6u1o2r6o0c0h,enFiactaelsofiFnlBulouregoiclhlemFiicsha"lsG,. A. Report Number 14, May 1, 1979 C3oMrnreelqluUensitveerdsietxyp,erMtaorvcehrv8i,e1w9,9"3Bioaccumulation Studies", Dr. James Gillett, oOeTHr ER Last changed: 5/25/00 OOOO 03668 I Rmttaniia TECHNICAL REPORT SUMMARY im TO: TECHNICAL COMMUNICATIONS CENTER -- 20136 Upir tdonbotho eof apra , nd cor nn0TCG.) Environmental Laboratory Fate of Fluorochemicals (EE & PC) No. 48765 0535 Analysis for Fluorochemicals in Bluegill Fish D. L. Bacon G. A. Vraspir/Arthur Mendel 014 76416743030 wconrvp 48838-18 0 oom Wcow ScumecaLy EN mEwFeesS Ia, Comma Gee Status Report Er7 ] A TORTAT TR R (250750wrk eae SaST Co eS ce:R.A. Prokop-236-: VARI.NLPooNBteohlahtpoernra-gs2aa1di-ao2-a0: 3A egw iy J 03669 I FC (Req. 48768)/GAV/AM -2- 5/1/19 OBJECTIVE Tfhiseh,purapftoesre oefxptohsuirseitnoveosutrigDaetciaotnurwapslatnotdeeftfelrumeinnte, ifbibolaucecguimlullated fluorochemicals. SUMMARY ET(xLPtEC)raiccntodsnitcaafitrneoedmdbtlh1ue0egppirplmelse(nufcgi/esgh)ofeoxfFpCo-Fs9eM5d,3t9wo2h3iDlaeecnadtFuC7r-1p4pp3mlaanonftdFeFfMCf-l341u22e38n.t were not detected. Eexttharnaoclt,s eftrhoymlbelutehgeirl;letfhisohxyeetxhpyolsedacettoatrei,vertolwuaetneer, (aEWn)d No fluorochemicals were detected. cxyolnetnaei.ned Aexpceorpiymeinstsa.ttached of CRL report 7124 which detailed GC/MS INTRODUCTION Tbyhefuipsthawkeereanrdepcolretaerdanc(e1).of sThpeecicfuircrenrtadrieoploarbteleddetafillusortohcehemicals Daencaaltyusresplfaonrtfelfufolruoecnhtemiacnadlsininfisfhisehxpproesevdiouosnllyy etxopToseendnetsoseeour Rtoigvrearphwya,terand(2)g.as Tchhrionm-altaoygerrapchhyr/ommaastsogrsappehcytro(sTcLCo)p,y (gaGsC/McSh)rowmear-e used. DISCUSSION OmuarnuDfeaccattuurringplainst lmaatneurfatcrteuarteesd fanlduorthoechetmriecaatlesd.effWlauteenrtusdeidschinarges etvheensteuabliloyassianytoextpheeriTmeennntess,seewerReiveerx.posBelduetgoilP.lE.fishA,notchheorsenlotforof i1sahh wweerree ethxepnoseadnaloynzleydtofotrheflTueonrnoecshseemeicRalisvebrywaatnerinv(2o)l,veTdhe itnhrtehee-reoxupteeripmreoncteadluresecatsioonu.tliBnreidefilny,FlofwisDhiwaegrreamho1moagnednidzeetda,iled wanhdilesosmoemoefofthethehohmoomgoegneantaetewawsasexterxatcrtaecdtewditwhittohleutahnyel (aRcoeutteate1), (RRoouuttee3)2).forAGpCo/rMtSioanndofTLCt.he lRaetstuelrtsexatrreacgtivweansinconthceentsruamtmeadry section. 03670 ae FLOW DIAGRAM 1 ANALYSIS OF BLUEGILL FISH 30 Pash ((21)) W"eTiagshuentze" s &e <7 _ (1) Weigh 2 g. (2) Extract (ETOAC) (3) Centrifuge (4) lsolate ETOAc Layer Route 3 oc FFoir.s02 AFu 33492225 Concentrate -- ac/us Tfce FC-95 FFCC--114238 (1) Weigh 2 g. (2) Acidity (3) Extract (Toluene) (4) Centrifuge (5) Tsollauetnee Layer (6) Mothylate -- GC/MS rGoCr (Ester) 03671 FC (Req. 48768)/GAV/AM 4 5/1/79 EXPERIMENTAL Tbtwyiovetlhlyeo,tsEnavnofidrsothnhimiperpnteytdalfiinsLahbdoreryaachti,ocrey,w,rwaepTrpheeedreifcnieshiavlewudemrieonnukmNepoftvoeilmf,breorzreen3s,pue1nc9t-7i8l, aOcnta.ly2z5e)d eighteen w(eNiovg.hed3, 251.9778)g.. TThweeleveiflfuisehnt-weerxeposceodllelcotted(Odcte,adantdo fish were sacrificed in Decatur. The river-exposed lot a(tOctD.eca3tutro. Oct. 35) weighed 33.0 grams and were 811 sacrificed Twaos siamnpalliyfzye,dthine were used: eexxapcetrliymentthealsoanmee mlaontneirs. disTchuessfeodl.iowTihneg ocotdheesr River River Water Water Holding Holding Tank Tank Exposed Exposed Fish Fish - eextthryalcatcet(aRt.eN.-EtAc) toluene extract PPllaanntt EEfffflluueenntt SS--11 EExxppoosseedd FFiisshh -- ethylac(eRt.at.e-Toelx,t)ract (P.E.-EtAc) toluene extract (P.E.-Tol.) TThweo 2fisg.h wpeorretiohnosmowgeerneizweediguhseidnginatToeksmeapraraMtoedeploSlDyeTthtyilsesuneemiczeenrt.rifuge ttounobeetsah.endotT2bwemorl.mlc'onsEca.cohfHCsplaemspptlliuecsidwae2smglmriaodxfeedeARtahpygplrraaodcxeeitmaatttoeelluyweenr1eewmieamrd,edeodanddte4o.d o1Vf0of,r0t0ea0xndrGppemln'aisce.edmiTxiehnre.geltahTshyselavcisaealtmsap,tleesraenwsdepreecttoilcvueeenlntyer.iefxutgTrehadectefstohrwyel1a5rceemitdnar.taewnat HePxtr5a7c1t3 wwiatsheKxispineeldecutsrionng cthaeptufroelldoewtiencgtoGrC. conCdoiltuimonns-: 6'xIn1st/r8." 10% Carbowgx 20 M on 60/80meshChromosorb W-A. Ing. Port - 8-.8. TFelmopw -- 24000cc/C.n,in.DefA.rgToenm/pMet-ha30n0e (C9.5,75)O.ven Temp - 150 C isothermal, uAnfdteerr NG,C panuarlgyesifsoroftwoEtAdeimeexntsriaocnta,l TaLCporatnialoynsewsa.s cTohnecenftorlaltoewding TsLpCotctoendflailtoinognssiwdeerestaunsdeda.rdsThoef Merck ailica gel plates. They cFCo-n9c5e,ntrFaCt-e14d3,EtAacndexFtCr-a1c2t8s ownerE.e were first developed in 10% EtOH iinn E5t0A%cEtaOnHd dirnieEdt.Ac.TheTnhethderipeldaptleastewsorewerteurnveidsua90lizeadndbydevseplroapyeidng with 0.01% New Puchein in water (3). Tehxeggitnoelduenuesinegxtrtahcet fwoalslomweitnhgylGaCtecdonwdiittihondsi:azomIenstthra.neHPan5d71t3henwith ND1C-300eleacntdro1n0%cBaepnttuorneode3t4ecotror8.0/90Colmuemsnh -ga1s2k'rxom1P/A8." 5.i5n.j.3P0o%rt 24t00o0luceo/nC.meine.xDtetrA.arcgtotsenm/wpMeer-teha30ns0eubm(iC9t.5,t/e5Od).vetnoThC-een11te0rtahlylCRaecIessteoaattrhecerhmaaAnlnd,almyeFttlihocywalla-tefdor GoCr/USGC/aMnSalycsoinsdit(iReoqn.s.No. C-50687). See attached CR Report No. 7134 03672 RC (haa. sor CAM ae sm Ry was that of reference FC-85. PE/EtAc extract showed approximately 10 ug/g of FM 3422. RW & PE-Tol. showed no difference. No FC-143 or FC-128 was detected by TLC of the PE EtAc, but a spot was noticed whose REFERENCES (1) a) M. T. Elnabarawy, "Bioconcentration of FM 3422 in Bluegill Sunfish and In Channel Catfish," EE & PC Report, 5/17/77. (8) Decatur Eeluent Pian Toxicity Test Protocols, dated b) A. N. Welter, "Aquatic Fate of A Fluorochemical, FM 3422," EE & PC Report, 10/14/77. Oct. 18, 1978 and Feb. 5, 1879; M. T. Elnabarawy. (3) 2J3., Sl1i21wic(k107a8n)d. A. Macioszczyk, Micrachemical dournal, da Vian stot /Hiaucll/ or chung framers C03673 -- CENTRAL ANALYTICAL LABORATORY Report No... 1030 Date. March28, 1973 Subject: Occatur Fish Bloassy. Requestor &- YraselleA; Mendel Request No. .S50887____| _ Report: INTROUCTIOK Dept. Name SEC| * Dapeq.Nove16m,1b97e8 r proj. No.15016130A00 TDSeeancmnapatluesrssewsPelarRneitv.errecwFehiiisvchehdweffrlreoowemsxptJhouessetrdeoquutteossittodwreosdtihfferfopemlraaannttfiwasnahdtebrltohsaeysssptyleamntsta:keefntfhlaeutentthe durlusaniznnoignngatthheiannGtoeC/HatnShdesayrnsiatvleeymrz..ed.TEtohlyuleneaceatxattreactesxtorafcttsheoffisthhewefrieshmweetrheylaantaeldywzietdh oisucssion wRTehMre.e saa#2mnpallaenysdzewdPe.rEu.esinFlg2sbce(olonetddhyiltAi.oWa.ncset#1aftoearnedcxotPlr.uEam.cntsA7)] .be(lmoSawetshpaylnldeostesAda.mVpt.loelsFuIeAn.aeVn.edxtPro2aEc.atnsd#)1 P.sEn.d 72 were analyzed using conditions for column below. OCIvonelsnut:mrnumeA1:n1t0:C12VI'asSro.ti5ha.enrm22a07l8800fGgo-ra2s010chm+rionm1ua0tt%eosgBraantpthheonne13140-o1n70AnCakartom10PCA/aln. Injector: 165 OCvoelnu:mn B5:0 =6!2265.5C.atCW12008C/m1i5n%.on Chromosorb Injector: 220 CDeatrerciteorr:Gas:VarHieanliuflmamaet 2f5onciea/smtiino.n at 290C HSIoonusdrt:creu:meanltlC:hesmaidmcupaPlloesntlwoe2nr1ie-zAa9tr1iu8nonmianssElsepcetcrtornomIsotneirzation, sample P.E. 2 Mass Rasngaes:als1o8 r-un617inwCeh.eai- cal Ionization with Wy gas . MtulltimpliSaarp:aragBoern:dixSCihnagnlneelStrconafmoe 03674 ' Report Mo. 7124 PY vn a nesuLts Samples R.W. #1 and P. E. #1 showed by GC/MS only the solvents used In extracting and mathylating: ethanol, ethyl ether, ethoxy ethyl acetate toluene and xylenss. It was necessary to alr concentrate the ethyl acetate extracts In order to obtain sufficient data to Interpret the mass spectra. The Tennessee River exposed fish ethyl scetats extract contained numerous G.C. peaks none of which appeared to contain fluorine. The components asphptehaalratteo,coarnrdeshpiognhdmotloecrueflearrenacleksanoesf 2f-rmoemtehbyolu-t3-oCcgtantoonea,t alceeatsitcCasyc.ld, & The plant effluent exposed fish ethyl acetate extract contalasd compounds wShiisscyh a{pIpseoarrochtaomiccoarlrseswphonodse tohigrheefsetramnceess oFfra2v-mmneitshysl-s3-nocstaanroensM, ssweeetiacnd 5s8e2anmw/ae,reniMt8roagsean caonndtaSi8n6] aie)lFu,Nwor3o9c3e3hmic(aFlOsSE (swnhiodsee) and PRt m3e0s3s2 (FfOrRaEgmeanltcsohol). pr Z-nathyl=3-octanone, and scatic acid. This fish bloassy showsd that extracts from the fish exposed to the Tennessee River water contained such compounds as ethanol, trl `ether, ethoxy ethyl acetate, toluene and xylene. The extracts from the fish exposed to the Decatur plant effluent contained FOSE amide, alcohol, other related fluorochemicals, J Iekmyiee I. n. Schrosptar sen 03675 Analytied Frm Bik BiconwabFi.s Studie 1 Bn4c4m70l6a1b26i90n ofpins BIE TPORN G 0. $1987 21PAGH | object: sari ASO PAR wet Ee WT pease s| meee te nr SbertTi ene, SESTOOPYAvan Tfoho TTLFSochlapel cSabflast matey N imnarbbleesd, bomE 1[0B FTionk5sE22 eon! heelaresproged om L The ok ofl _324doy eufpsure were dried of wh Dn ASl ihe ". Le3.d95a,! AcT elonEe_only EM47-752. L6A7c"l2os The +h (5 lok) di lw25m - p dQilg1rsteld nToSrSF E Sm Lw ALed OT mt AE 2E) Ohe 4ma 3 2 x 50at wih afipueke ok wnHet, wadCerc wuityeCrasfes led Y Al samples Gl-- ed see FC [J3[7L +o rei. d Water = m/l PM3922 Control - a Acedone- NI Ed - maf - deved 13) Condvol = icien; Actlone- 0.000mgfy . FM-322- 0.0Tiym lt F-39422. 0.161 "tm'/e - 03676 son Kg Vos HT ar ne owe308] . orn } emoiserNo._Q4 06 1zio0 owe 3M TECHNICAL NOTEBOOKho. Pace AE s|Retwence: LR 3114 by chounel way te cad disn debormme the uptake in od [000 hem tank of ozs (dale 33day Very He Loh Led age 2! Figosare Jo TES Samp Li =Tewste t walWaleed r(10O0ita|) ct wh Fish 9 - 32 \ Fuh wee gathed - Fgh 24 J Fsh 8 2s 7 Hl Tl der sonple wes akual wl Ial cbpur [0a hyo ) Fish wee digodd 1p bal mek of Wirsese Ll Thm ecterclu 20%kol b L will oat 1m @ Bdadk Gla See intqrale ceports 043-048 p Yo yils Em Test wher 48Lnglt 5 Fik - 030mgly - Fak gutted wf, { Fh - 028my - noon od ont mtdTRF 7 Ex - Cdbmfy vo Tho dost winter wes Lit +h Mill, 0.220 Lolo irlegrely repect 049-051 do iki 0,01 mylf. - C03677 se AOpen oue3Bf7 dowPSRoOJeECTNo._497012 b0 Pac 3M TECHNICAL NOTEBOOKDoNoe,s 2/4432 TT es He md SETEE EL Ee | Retences ~~ LB 30+1T4h d part of He uolike br Sb Te4w Fay. Ufo.6"% L "arkanalSolo teTel hd ' ) Sampls submited+ Conlrol Waly - Repro ok lor Erol gute wl '. s CCoonnletorl| Fish Fisk * %2 $e 80 Egood fb 0 3.90 ler pls Fepoued Fish "2 das 2 flo 36 atte Fak dgat as eorlior ond oxides wih foal ocf. y| See al Ged Tg repost 06/- 007 wt 0.0% J i weld Condo! Wool Pe Woh - <00lmlt 033 milf Fm-3422 . {. a-t - 000067mfy a 0 (-z 0,000 mls . Fol 0,029agly. Fz 0.03 wyly, " fe lore C03678 oe 3fof8 gamuraces | Sib PROJECT NO.. Objet: 3M TECHNICAL to. NOTEBOOKNo. PAG Hers lon TE s|Ronces Spee gueskin wos Jom) [5m V0 fifo" raged do fh elmbaem vadine volbiy usec! oFHe fo A Smit soln of FM-3422 jo Hop wos made 0 dspersifly A Eman, . edo we b a 1,2 w= 3 elomiding weomade with iy J Imi Jour sali GL = Td reper 052-057 ) Ser a i The pil wes rt. gn - 2 BB Toda=l 0,438 |. 7 22 lp > s(eecoverFyo Te ba p Sams Sproecedusre woseCded a rqoarls s0a5l8-w0p5l9e sof PY rE yield rs 85% 9% 75ple { 2d xz WL 084mU 8dmb N 3nd 17, Otugle * ~ soe KO Vout 03679 ou oI , FRolECT No.444790100612000 PA 3M TECHNICAL NOTEBOOKro. Her a ot 5| Reference: BEST COPY AVAIL ABLE d3ostm(pleSs tleweere ddrownen Fwoenre tthueber FMo-f3tH2ze sfainle 0 levels as fe v Pa-342e oaks samplscmd will be anslyeed 0 See ODaFy 8 8 28 oe? is "Mo gue me Zdigdd beg Sak samples ) Duy 8 Cnbeol 3h LIS Eupoced Fish 0.97, Vater L on 3[ale & Lil sample - Thay oF pate, ie dak we "- Conlral 3JMI7 -3Fak wis L433. P| Et 3prir-atss who vime, Bl { FBaoihpdstsaadmTloacfsbworeAsoctelloerackrewdonrdwdibh ,pHS2oaslejautslepso"vbiimshOof S 4etGpnoherklk eh 8 of Fisk sume Lot Tdagader coporkegsp)s-a Resulhy bot 36 Wo back \ : Evposed tole Fk fdas 387+ total Dhbon3s0 0473 Tub BT Olism "= pe GMhBs 3s Evposed Fak 14 dogs 3/4f- 1172 $m - 0.0Tmly fat sonar Dy Visrpie__ _ no wa MT Slants mah See PME $4 for 2lday Exposure. oe M21F7 03680 - 20/77 PAGE Project No._23706 12600 MTECHNICAL NOTEBOOK No. 4 : 1 Subject: FM-3472 Uplke 1a Channel offish Date: Ee 5 Slot GCoomlyss of wold aed Fi Samples xubaifed by A) uelbe Br FM3922 BX Fleder Cope | Retorence: B.C. Coding sed for Chvomosorl W AW (Oss shudy: Mesh Column 64" SS CW20m - 67% on column ,yyechin Flow: Avge nw Temps Tog. Reed 200c Def. 300C Oven (70C Toobhormu oSR handads run on caeceh doy of anoles, SH. W1H Sju eeft tacee lde ond of sal Mall silh pda & sol ovgomis. 100al unter \ sawple exlaaced dilded 4s 10M. 1 Tose semgle Jism in efi! slate pre to submit! fv GC. opmoluis. The dx eH! arelale wos dronfend do vel wmelrne s| flasks vd subreguad wiches mode with EA. Hen Alu fukn The <haly iistoad Uptok-e coded "U" pnd clesrame coded "C17 ) CL. ambpsis of Zl Shs. 10pm Fn-3627 Bi Tndpalor TERT Vales. Ave. 1,261,380 V Walder Spmple 20hv Sonmplesizs ppm 124,838 Uptake [A pom F034 5 Bhoat)e. 1100a0!4 10+ foul 0.347 0.342 430% [ Top. &e' 10m! 0.51% 0.498 Fisk 0 20h. whole | Whole2 Joh Whe! 5 Wholez wh. , ont 18.1; 13.6%, 12185, Frat Ua, ' Soar Soa " Fn 342% (u1y) 626 G06 752 730 530 484 596 536 soe BO Voopn tens tens ATT Ere nll oue MT 03681 - Aes he 3M REPORT: BIOACCUMULATION STUDIES March 8, 1993 4. ReportNo.14(5/1/79) *AnofaFuorlochyemiscalssinBluegil Fish" are prAelstehaotuegdhintahisverreypomratrogfifnearlsiszeodmemadnenteari.ledItexgpilveasnatthieoniomfpraensasliyotnictahlaptrdoocse-d(ubryedsr,otxhyeetshaymlpatlieosnooufrcNe-sEatnFdOdaStEa alcobol occurs fa the environment. 3 03682