Document bOVmrLnNNyGYkKQxeqpE2YvbZ
AR 226. 0686
ACUTE TOXICITY TO FISH
EST SUBSTANCE
Identity:
A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-185F. (1Octanesulfonic acid) (CAS # 2795-39-3)
Remarks: The 3M production lot number was 1-2303. The test sample is FC-185F, cc 817-19. Current information indicates it is a mixtureof 4% PFOS, 36% diethylene glycol butyl ether, 29.29% water, 14% ethylene glycol, 8% Sultone foamer, 8.35% sodium octyl sulfate, 0.3% sodium lauryl sulfate, and 0.06% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrationsof impurities. Data may not accurately reflect the toxicityofthe fluorochemical componentofthe test sample.
METHOD:
Method: ASTM-E729-80
Type: Static acute
GLP: No
Year completed: 1982
Species: Pimephales promelas
Supplier:
Analytical
Dale Fattig Fish Farm, Brady, NB
monitoring: Temperature, pH, and
DO
Exposure period: 96-hours
`Statistical methods: LCs values calculated using both moving average
angle and probit programs in conjunction with the 3M TRAC Computer
system
Test fish age: Juvenile Length and weight:
Average length = 38 mm
Average weight = 0.38 g (wet)
Loading: 0.48g fish/L
Pretreatment: None
Test conditions: Dilution water: Carbon-filtered well water
Dilution water chemistry:
Hardness:
252 mg/L as CaCOs
Alkalinity:
225 mg/L as CaCOs
Lighting: Adaily photoperiod of 16 hours light and 8 hoursdarkwith a 30 `minute transition period was maintained throughout the testing period.
Stock and test solution preparation: Test solutions were created by direct individual weight additions. Concentrations dosing rate: Once
000119
EStxapboilsiutryoefvtehsesetless:t cAlhle-mgilacsaslasqoulauritai,on3s5:xN2o0tx2n5otcemd containing 16 liters of
NNteuustmmbbsoeelrrutioooffnfriesphlipcearterse:pl2icate: 20
WNautmebrecrohefmciosntcreyndturraitnigontsh:e sftivuedyp:lus a blank control
pH range (0-96 hours):
78..07--88..22 ((c3o7n0trmolg/eLxpeoxspuorseu)re)"
:
Tempor1a7t-ur2e1rCange (0-96 hours):
Dissol5ve.d89ox.y2gmegnlrLa(ncgonetr(o0l-9e6xphoosuurrse)):
* 370 m1g./3L-9(.s3ecmogn/dLh(i3g7he0smtgc/oLnceexnptroastuiroen))*exposure values used
because the highest concentration used resulted in total mortality by
24 hours.
aRlelmeaxrpkossu:reThcoendciesnstorlavteidonosxeyxgceenptcotnhceenctonrtartoilobnyw4a8s bhoeulrosw. 6T0h%issmatauyrahtaiovnein contributed to the observed mortally.
RESULTS ENloemmiennatl vcaolnuceesn:tr9a6t-ihoonusr: LBCk50con=tr3o4l4, 1(0301,8 -1553,762)40m,g/37L0, and 560 mg/L.
Element values based on nominal concentrations.
`RSeumbasrtkansc:eTReesmtianrgkwsafsielcdo.ndTuhcetveadlouensarmeipxotrutreed oafppalsydteostchraitbemdixitnurteheaTnedstnot the fluorochemical components alone.
CONCLUSIONS
-_
mThge tewsitthsaam9pl5e%9c6o-nhfoiudrenLceCinftoerrvfaalthofea3d1m8itnon3o7w6wmags/Ld.etermined to be 344
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
HReolwiaebvielirt,y:saKmipmliescphurriatynkwiangs=no2t. pTreospetrilnyg cmheaertascttehreizcerditaernidaftohrequsatluidtyy ltaecstkisng analytical confirmation of the amount of fluorochemical proportion in the solution.
000420
REFERENCES
_
Testwas conducted by the 3M Company, Environmental Laboratory, Lab Request number 7544, 1982.
O-- THER------------------
Last changed: 6/26/00
000172
romerirane
TECHNICAL REPORT SUMMARY
"3/1/82
70: TECHNICAL COMMUNICATIONS CENTER -- 201-20N Su(immmpoarrtaynta=vIafsretporrtosmprnifnotremdeatniobnotLhisiodnesoofrpTaCpCer(,s+e6n5d48t)wocopsto TCC.)S?ee"GuidlinesforCompletion of Technica Report
moatEnvironmental Laboratory
hem!
S
(EE & PC)
0535en
9972210100
t Toxicity Testing: FC-185F
EA Reiner
2AOA hfereer = 2-20
(cc817-19) L 1-2303 LR 7544 007
.
aT09362 C=
[---- TevR wonoen .
Toom
Teac
ree gs
ER ICPSAT INE the acute
varying concentrations
oafquaFtei-c18I5CFso(,cchfloyll1o5w)inLot
284ur5e 5. to
EE & PC-Div.
(Env. Lab)
Toxicity (Aquatic)
TE
The acute static aquatic 96-hour LCsqy values obtained for Fathead minnows (Pimcphales promelas) following exposure tc
aFnCd-18357F2 i(n333re-p4l1i5c)atmegd/l.expeTrheimecnotmsbinweedre 9633-9hou(rnotLCdseqfiwnaesd)354
(0) (not defined) mg/l as calculated using the probit analysis. statistic.
Data generated include LCsg values with 95% confidence
limits
.
pneeel]
000122 i
FPCag-e18528/ANW February 1, 1982 concLusTons fInor thfeasteheardeplmiicnantoewds 9(6P-ihmoeuprhalLeCssg parqoumaetliacs) tweesrtse, 33t9heancdalc3u7l2atmegd/l.values MtoateberiaplrsactpioscsaelslsyinngonLtanoxiicabaoosfed onPRISthOeffNeIrOSofH mcargintietruidae faorreaqcuoantsiicdered toxicity interpretation. (2) InTRODUCTION Twhaeterteastt asmubbisetanntcerooims taemfpoeamrya,turcel.ear,Tesdtasrkpearmfboerrmeldiquoind,thissolumbalteeriianl etvhealubaatseids oiftstphiostenrteipaolrt.aquatic toxicity and the data generated form ACCLIMATION AND EXPERIMENTAL DESIGN TdheescrtiesbtedprboytoAcSoTlH-Eu7t2i9l-i8z0e.d fo(r1) this study was modeled after that Thhaetchfeartyhea(da):minnSotwosck (FPIiSmhepwhearleesheplrdomIenlass) flvoewretohbrotuagihnedsysftreomm a private 1f4i-b1e6rgldaesgsreehsoldC.ing Atdaaniklyfipllheodtopweirtihodcaorfbon1-6fihlotuersredliwghetll-anwdate8rhoaturs adacrckl,imawtiitohnaan3d0-mteisntuitneg tprearnisoidt.ionThpeerifoidshwawseremaifnetdaiTneetdra-tMhirnoug(hb)out the dpaeirliyo,d. fooFdishbewienrgewiatchchleilmdate4d8 htoourtsestpriteomrpetroataunrde tfhorroug2hoduatys thperiotrestto use in assays. Tcwoenncteyntr(a2t0)ionfaotfheacdhemmiicnanlo.ws, Aulnli-fgolramss inaqusairzei,a,we3r5ex20txe2s5tedcmatconetaachining E16ishlitweerrse rofanddiolmuleyntallpolcuastetdoxitocanetachcotmepsrtisecdhamabesrtuwdyithchianmb3e0r.minuTteesst cfoonldluowcitnegd attoxi19c+a4n2tdeagdrdeietsionC.. MTohretaflaitthye,adtmemipnenroawtuerxep,erdiimsesnotlsvewdereoxygen olreveuln,tilandtotpaHlomforatllalitteysthasdolouctciuornrsedv.ereGmeenearsaulredobsaetrv2a4t-ihoonusr wienrteer-vals rbyecoerxdpeodsurreelattoivtehe totesstwimcmhienmigcapla.ttern and behavioral changes induced Tchaerboanc-uftieltsehroerdt-tweelrlm swtaatteircofbiokansowsnayscomweproesitpieornforamneddcoonnstFaCn-t18q5Fualuistinyg as the diluent (Tables 1,2). ba)) CDoamlemerFcaitatlig FiFsihshFoFoardm,ofBrkandoyw,n Nceobmrpaosskiat.ion.
000123
FPaCg-e1853 8/ANH February 1, 1982
Tfhoello9w6i-nhgourFC-f1i8sh5Fstluodgiaersithwmeirce cpoenrcfeonrtmreadtiionnsr:epli1c0a0,te 1u55s,ing240t,he 370, and 5v6a0luemsg/lp.rediTchtiesd rafnrgoem pofreldiimliuntaiornysewxapslorsaetloercytedtoxtoicibtryackteestts9.6-hr LCsp mLoCvsigngvalauveesragweithang9l5e% acnodnfipdreonbciet plriomgirtasmswerien ccoanljcuunlactteidonuwsiitnhg btoheth 3M TRAC Computer system.
Monitoring equipment used temperature--compensating
piHncmleutdeerd, ASaTHYeltlhoewrmoSmpertienrgss, dOirsisoonlved
oxygen
meter, and a Taylor temperature chart recorder.
RESULTS
TShveerafgaethweaedighmtinnofows0.4(gPrimaempshaalneds
promelas) used in these studies had Temgth of 3.8 cm. The loading
an
f(a1)c.tor Tweamsper0a.t4u8reg,/l;pH,theandmadxiismsuomlvreedcoOmxmeyngdeend vallouaedsingandfarcatnogresis ob0t.a8ing/eld
duirsisnoglvetdhiosxysgteundyvwaelruee:s ra1n9g+ed20Cf,rom7.71-.83.-25.9PHmgu/nli.ts, Thaensde 9l6a-thtoeurr values
calculated out to a minimal oxygen saturation of 14-63%.
3In72thmigs/l.repliCchaetmeidcalstupdoys,sestsheingfisahn 9L6C-shgouvraluLeCsinvatlhuiessrwaenrgee a3r3e9 and considered to be slightly toxic to the aquatic environment (2).
TEhlenabdaartaawyreapnodrteRd. hR.ereRionbidaeraeu.baseAdccoomnpasntyuidnigesdaptearfosrhmeeedtsbycomH.priT.se oLraibgoirnaatlorydatAar.chivAecoSpysyteomf, tBhiusildrienpgort2-3iEs. filed in the Environmental
aIbtsesnhcoeuldof beinfnootremdatitohnatretlheatiqveencrtaotetdhere"spuulrtisty"wearned vtehpeort*esdtabinilitthye" of
tthhee
bcahseimsicaolf
sthuebst"aonbcsee.rvedThemorrteaploirtteyd raLtCesso" vaanlduesof wethree
calculated calculated
on
initial concentrations of FC-185F in test solutions.
REFERENCES
(1) AAcSuTtHe-StTaonxdiacridtyPTreascttsicewitEh729F-i8s0h.es, SMtaacnrdaoridnvePrrtaecbtriacteesf,oranCdonducting APmhpihliabdiealnpsh.ia, AmPeAric1a9n80.Society for Testing and Materials,
(2) CRhergiisstternyseonf,ToH.xicE.,EfafnedctsE.ofJ.ChFeamiirccahilldS,ubsBtdasn.,cesp,. X'V76aEndditCiio,n,U.S. DfeoprarOtcmceunptatioofnaHlealStahf,etyEdauncdatiHoenaltahn,d WWealsfhairneg;tonN,atiDo.nC.al (IJnunset,itu1t9e76).
000124
SSAAMMPPLLEE TDAAKTEE:N: 1/32M8/B8l2dg. SAMPLE
PARAMETER piRtesidual Chlorine CAomnmdouncitaivNiittyrogen t-TAotlaklalSionliitdys TToottaall HFalrudonreisdse TDoitlaulte CCoOlDiform Standard Plate Count Aluninum as AL*
ABorrsoennicasasB*As* CCoopbpaelrt aass CCou** Chromium as Cr* CIarodnmiuasm aFserca* Nickel as Ni* SLiealdveras aPsb*Ag* Mzeinrccuarsy azns*Hg* BCB's** TotPaelstOircgidaensochlorine** TOortgaalnophosphorus**
Pesticides
TABLE 1 WELL WATER QUALITY
2-3E, St. Paul
DATE 1/28/82 (TAKEN FROM ROOM 5)
CONCENTRATION
WENAVT.ERLAQBUALITY BOOK RFF.
7<.07.02 mg/1
89
<303.01ummgh/ols/casm Nat 25C 89
232135 mngg//11 as CaCO3
88
205.202mg/mlg/1as Caco3
89
N<e0g.a4timvg/e1
109
21 per ml
10
0<.02.00m5g/1mg/l
1144
<<00..202 mmgg//11
1144
<<00..0032 mngg//11
1144
<<00..0012 mmgg//11
1144
<<00..0025 mmgg//ll
1144
0<.00.502mgm/g/1l
1144
<0.2 ug/l
14
Np
95
ND
95
np
95
***sSaammppllee DDaattee 13//248/8/181
000125
TABLE 2 ENVIRONMBLEDNGT.AL2L-3AEBORATORY
ST. PAUL, MINNESOTA WELL WATERFOSUURPTPHLYQUAFROTRERALQYUATRIECPORTTO,XIC1I98T1Y TESTING
PARAMETER TOasTALmg/HlARDCaNCE0S3S* TOaTsALm:g/"lM* CAaLCOK3ALINITY* ECLOENCDTURCITCAANLCES*PECasIFIuCmhos per
cm? at 25C
TOasTAmLg/SlOLIDS*
pur
STpeArNDA1R.0D mPlLATE COUNT***
Nov 2 248 228 330
320
7.6
2
NOV 30 252 226 340
300
7.6
10
DEC 29 256 221 380
AVERAGE +8) 2524 4 225 + 4 350 + 27
308 309 + 10
7.7
7.6 + 0.1
27 13413
CRITERIA OF CONSTANT QUALITY
* Maovnetrhagleys.rang(eAsSTsMhoSutladndaberdleEs7s29-t1h9a8n0.1)08 of their respective
** M7o2n9t-h1l9y80r.a)nge of pli should be less than 0.4 unit. (ASTM Standard
*#%
Tpoetralml.bact(eUrSiEaPlA-d6e0n0s/i8t-i7e8s-01s7h,oulpd.
not be 101.)
greater
than
500
organisms
000126
"
ENVIRONMENTAL LABORATORY EIN veTOL - SRE TES)
wn SAUL
oe TesAt cuQ te L ScAU me aR ter, ial Tested FC-18SE_oo 817-19 tot _|-230%
st Organism EATHEADMINNMos Source TRAY,NER Pate Rec'd 1) JX atch + 5
9.4.BB0r)an,s Avg.Size ZBcn to. Organisms per dose20) Loading Factor 0, 4p (&%)
oh Disease Treatment: _\ IONE.
st SolutionVolume|pLiters/All-Glass Tank Tank Dimensions: 35x20 cm and 25 cm deep
meStarted}! 350PM__DateStarted | [2p JA2, Date completed|/ 20 /BZ,
ation Water Carbon-iltared well water (tarp. /%/_*C., 0.0. 2,5pom o_8.2s
westigator RAJU) 3
105, (958 J...A
.
SE Er PEEE EE TE ark [Initial| Total
24 Hours
18 Hours
J. 5
LUE
fo.
Conc. | Amount| Total No.
0.0. JTotal No.
D.0. Total No.
D.0.|Total No.
D.C
oT enol ==] 0 kill 0 Biles] kdud o kis
|seolean] zo Baleol20|-+ [20 I+120[4 1 111 T1171 111 11
ne ce bd Tents Ta TL neU IDui)WIEMAITTESST," MATER 15 A EOMMY CCAR DARK AMINER
LQuID, SeLuBEINLHTER ATAMEIEAT Zoo TEMPRITURE,
acondary Effects (other than Mortality):
ECs0_
No.
Symptoms or Reactions
24-Hr.
48-Hr.
72-Hr.
96-Hr.
otxol EbJhowe |Nowe | Mos
ave
|Mowe |Nowe |Nowe| Na
' "-- I-- 1
=
Tre oI SoA HEED wnYsa,
peTestQiaun,NeueSame,Material Tested FC-1RSF ge B14-1Qiot_|- 2 X0%
st OrganismEwTuEAD Minow Source_BRAoY, Neg, Date Rec'd|| fxp/g1 Batchpoe
9.04 ,BBt gra.ns Avg. Size
om Yo. Organisms perdose 2()Loading Factor.
al &
ee 2st Solution Volume||pLiters/A1-Glass Tank Tank Dimensions: 35x20 cm an and 25 cm deep
Llution Water _Carbon-filtered well water (Terp. 7 c, 0.0. 2%pom, oi_K2Z)
westigator.
3
Rats Hee Lint a)
eg = Meee ss Gi Sen Sey SUGES un i,: fon ANGE TL Zh
oUt CoenD
less
E Era E Ee EE TE oT onwed--2]0RIB]0 leiles] kdod 0 hdse
clam
-- | Ribot]0 leluz] Rdsd ~ kd
=>|740 |=ato] killed 0 RBdal bdo]
Talis
ozo [=o 20 BMI 5 Bal8 kis]a Ki:
sD| 0 lea] Wo 2d 20 bold] 20 H-120 H
1 1 111-1 T171 71 1 711
Test Tem. C |" 779 | Zo | zagc. |
o
Initials & Date
[Xs[2r7 s 1RE8 PRE IXS 29/82832 [IE
TENTS TF[ine bai) MIGIGHTE, EATMATERIAL |S A TOMIY #IESR DARKAMBERLIZ ID
DOUBLE 1M WATERATAMBIENTROOMTEMPERATURE.
scondary Effects (other than Mortality):
ECs,
----
rol
Nene |Ame | Mone | Am
mo d
| Nowe |Awe |Awe| Ada
D |Sus [Ne owen |5c|n Ise| 20
2D |sim |Nv owea |1e 0 n |e 20,| 0
= L<u|e Nowe e |t 10o |m 20s| 9
DD ee[r Naes 115t | 1e 20] Z
=> |~ [Nowe~ IT---- ---- 1
Soesss
ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET
DEFINITIVE (FULL-SCALE TEST)
ves weston,Kure ome.
MATERIAL TESTEDErCc8=17I-1B9SLoEv |-2303 TEST ORGANISMEATIESD MINNOWS
1Csq's (95% CONPIDENCE LIMITS)
PROBIT ANALYSIS
we
35Bndl| 353uafl| 33 wld
i} (No 1amrrs)| (No ume)|(No amis) m5wall
UN D HTS ll
Sze] (No0 Wi urs)
peassagl CL](e=5-oug
MOVING AVERAGE ANGLE MEtiOD
Sul| STOW| STOW
WC ead (321-43) (5271-43 wl) (200-38
SL wf
OD
AN | BBDafl | STSuql| S55aql | (318-370 weld)
set) (20-4otgeD (46-2) (532 Sa)
ol
000129
.
AQEUANTVIICROTMOEXNITCAILTY LADBAOTRAATSOHREYET
EXPLORATORY (RANGE-FINDING TEST)
LR, Hs 4A
Type TesRotuteQSri Ameu. Mn ater, ialTested FC. BSF(ccB17=(qhot_|=50:
Test OrganisnEATHEAD MINNOWSource RADY, NER. Date Rec'd. 1/20/8B1atch rok
Avg. We. _0O, XE)
grams Avg. SizeZyC}M No. Organisms per dose 5
Test Solution Volume3Liters/All-Glass Tank Tank Dimensions:WIDE-MOUTH | GALLON GLASS
Time Started||: |SAM Date Started | 9)A2
Date Completed 24/82,
Dilution Water_Carbon-filtered well water (Temp. 20 C. v0. 88 pom. m8
ep fg Leg, 5% Confidence Limits)
em ||{ zo zize i i (Full Signature.
Tank[ame [[ oTomrae Total 74 ows0.0]
No.
8
ememn||
--5]| Added
0 loilusl] No. Dead| pl ppm.
Cleorsa--1
0 Bled
A Lio 110 Io peal
a Too =n I 0 Wes
a sno soo[| 45 BLA
A [inno lenoe 175 BUZZ
a 12000 [liac00 || 5/5 BRO
Co
rr
TotaTE ours 0.0] Totar 1rspo. [[rot=ar o
o lrolesl] o No.Dead| pH | pp! No.Dead beglb pi ppm||No.Dead pH
0 gill OBll 0 Je
o
alle} lajWAl
0 0
14S-2-- has o bs
os[29115 SIs
S75 =
Ss [oS --lls/s|
Fs| [I &/s[4
[&/5[-
rr rr
a
Date [|rJe Jfa2/o/BaZz NFX ars fasfBRz _T14 S 1/aa2/f82e0e][M14
Comments: *|uruinudiyWEIGHTS,TESTMATERWML 1SAFOAMY,CLEAR,
Effects other than Nortality
ec,
Ti Tm ee sions|| see, BE an
No. Affected
lconezon HfC f oree||Anw. e || Mowe | Xa Ne
Tal 2a [I
AweII tpwe [Mae 1 Aur | Moe [Awe [|
maAll ll--1 ame I" -- 1 1 1 1
[sal
--
-- ----
000150
