Document bOLVnEEEkV0ONDZmNXQ0GV8g
ACUTE TOXICITY TO FISH (BLUEGILL)
ACUTE TOXICITY TO FISH (BLUEGILL)
I TESTE SUBSS TANCT E SUBSTANCE
IIddeennttiittyy:: AAbemmriiexxftteuurrrreeedccootnnottaaaisinniiPnnFggOpSpe,errffFlluCuo-o9rro5ooo,ccottarannaeesssuuallfcfooonnmaaptteeo,,newwnhhiticcohhf mmFaCa-yy60aa0lls.soo {(bh11ee--pOOtrecactfdteaearncnreaeelsdsluuultooflfrooaonn-sii,ccPpaaFcocOitiddaS,,s,s11F,i,11Cu.,m-229.,s522a,.l,3t3o,.,3r3C,,a4A4s,,S44a,.5#5c,,5o25,m766.9,p656,o-,77n3,,9e77.n.,388t.),o88f.,88F--C-600.
heptadecafluoro-, potassium salt, CAS # 2795-39-3)
sRRaeemmmpalarekrksiss::F_C-6TT0hh0ee. 33MMCurpprrreoonddtuuccittniiofoonnrmlloaotttinnouunmmibnbederircwwataaesss nnitooitts nnaootmteeiddx.t.urTTehheeofttee1ss.tt0%
osPPcaFFtmyOOlSpSs,le,ul88fis11at..F2e2,C00%2-%6.00ww0a0a.t%teerCpr,,urro11rp22ea..n0n0t0e0i%n%Sfouddlriimteeottahnhtyeiyollefnenonaieenmdgegilrlcyy,acctoo1ell.sbb0uiu0ttt%yiysllsaeeottmdhheieirxur,,tmu11rde..e00co00y%fl%1s.ssu0ool%fddaiituuem,m
0o.ct8y5l %suxlfaantteh, a2n.0g0u%r,p0ro.p1a%neN-(S3u-lctohinoerofoalalmy)erh,e1x.a0m0i%nisuomdicuhmlordiedec,yl0s.u8lf0a%te,
0sstt.a8arr5cc%hh,,
and 0.05 % benzotriazole. xanthan gum, 0.1% N-(3-chloroallyl)
and 0.05 % benzotriazole.
hexaminium
chloride,
0.80%
cTThhhaeeraffocotlllelorowiwizinengdg sscouunmmcmemanatrrryyataaippoppnlliioeefssittmoopuaarimmtiiixxetstuu.rreeDawwtiiatthhmiiannyccoonmmoptplelaetctecelulyyrately rrsceeahffmalleperlcactetc.tttehhreeiztteoodxxiicccioittnyycooeffnttthrhaeetiffollunuooorrofocicmhheepm muiricictaaileiscc.oom Dmpapotoannemennattyoonffottthhaeecttceeusstrtately
sample.
MMEETTHHOODD::
TMMeeesttthhootdyd:p:e:NNoSotttatnniooctteeaddc.ute
TGYGeLeLaPsPr:t:tCyNNopmooep:lSettaetidc:ac1u9t7e5
YSSSupeppeaepclrciiioCeesrso:::mLLNpeeolppetootemnmoditi:ssed1mm.9aa7cc5rroocchhiinruuss
AAESnxnuapaploylpystltiiueiccrraa:ellNpmmoeortoniinnotoidott:oerridSin.n6g-g:h:oTTueermsmppeerarattuurree,, ppHH,, DDOO
STEStoxtasatpttioissfsttiiuisccrhaaelal gpmmeee:ertitohJhduoovd:des9sn:i6:l-PPehrsorooubbiristt aannaallyyssiiss..
Average Test fish
Average
LLaeegnneg:gttJhhuvaaennnddilewwseeiigghhtt::
WLLeeeinnggghtthth ===101 .ii5nncgchhrams
Loading: Not noted.
Weight = 0.5 grams
PTPLreoreseatttdrrecienoaagnttdm:mieNetninotott:n:nsNN:ootoettdnn.ootteedd..
Test coDDDniiilllduuuittttiiiioooonnnnswww:aaattteeerrr::cCChaearmrbbiosontn.r-ffyiil:tteeNrreoeddt wnwoaatteetedr.,r, CCiittyy ooff SSt.t. PPaauull,, MMNN
LSLitiggohhcttkiinnaDggn:i:lduNNtotiooettsntnnoowsttoeaeldtduetricohn epmreispatrrya:tiNoont:
noted.
Test
solutions
wore
created
by
ddSCiiotrroneecccctetknwwateeniridggahthttitessosnaatsddsdddoiootliunsotsinion.s.ng
preparation:
rate: Once
Test solutions were created by
SECStxtoaapbnbioclisleiiuttnyyrtoreoaffvtiettohhsneessettleedssso:ttsNccionhhtgeemm nroiaticcteaedal:.l Ossoonlcluuettiioonn:: NNoott nnootteedd
NNENuuxupmmmobbbseeeurrrreooofffvrfreeiessppshlleiicpclseaa:rtteeNrsse:o:ptlOOninncoaeetteed:. 20
Number of fish per replicate: 20
Exhibit 1119
1111199..00000011
S3MM_MMNNO0T1G65S8E221111
Number of concentrations: 5 plus a blank control
WW NauatmteerbrepccrhhHoeemrfmiacisnostgntrercyye(0ddnuu-trrrai9intn6igoghnttohshu:eers5ss)tt:puuldudysy::a blank control
pH ran777g..1e.12(--0777.-.32296(((cc2ooh.nn0ot0trur0oorlslm)eeg:xxlppLoosesuxuprroeesssu))re)"
DDiissssoollv75ve..e2dd6--OO77xx..y3y0gg(mee2gnn,l0L0rra0a(nncmgogneegt/r(L(o00le--9exxp96po6osshhuuoorreuuer)r*sss)))::
Temper445a...56t5u--6re76...000(0mmm-ggg9//LLL6 (((hc22oo,.u0n0r00tsr00)o:lmmeggx/LpLoeesxxupproeosssu)urree))**
T* Veamlpuee77sr22aOftouFFrrethe(02-.09060
hours):
mglL exposure
(third
highest
concentration)
wcw* oeVenrraceeleunuuetsssreeaddftoirbboetenhcsceaatuu2esss,t0eee0dtt0.oottmaallgmm/Loorteratxalpiliottyysuoorcceccuu(rtrhrreierddd
in the highest highest concentration)
in the highest
concentrations tested.
RReEsSUwLrTSs
0000
4NN,oo0mm0i0innamall gccoonncceenntrtraattiioonnss::
8lank
Blank
control,
control,
1.000,
1,000,
1.500,
1,500,
2,000,
2,000,
3.000,
3,000,
and
and
4EE,ll0ee0mm0eenmnttg/vvLaalluuee:: 9966--hhoouurr LLCCsso0 == 11,,550000 ((11,,228622 --- 11,,775555)) mmgg//LL
EElleemmeenntt vvaalluuee bbaasseedd oonn nnoommiinnaall ccoonncceentnrtraattiioonnss..
RRSueebmmsatararkknssc::e TTReeessmttiainnrggkwwsaafsiselcdc.oonnTdduhucectteveaddlouoenn rtthehepeomrmitixexdttuuarrpeepalaisseddseettosschcrraiibbteemddixiinntutthrheee TaTenesdstt
not the fluorochemical proportion alone. Substance Remarks field. The value reported
not the fluorochemical proportion alone.
applies
to
that
mixture
and
coCnOcNlCLuUsSiIOoNnSs
`T1Th,h5ee00FFCCm--g66l00L00w99it66h--hhaoouu9rr5LL%CCssco0onfffooirrdLLeeenppcooemmiinisstermmvaaaclcrrooofcch1hi.inr2uu8ss2 wwtoaass1.dd7ee5tt5eerrmmgm/iiLnn.eedd ttoo bbee
1,500 mg/L with a 95% confidence interval of 1,282 to 1,755 mg/L.
SPSauuublbm,mitMittitenernr:e:s33oMMta,CCoo5mm5p1pa3an3nyy., EEnnvviirroonnmmeennttaall LLaabboorraattoorryy,, PP..OO.. BBooxx 3333333311., SStt.
Paul, Minnesota, 55133
pDaAtTaAQQuUaAvLrITyY
RmReeeltilhiaaobbdiolili'ittoyyg::y.KKiliTimmhiiessccshhamrraapnnlkkeiinnpggur33i..tyTTwhhaeesssnttouutddyyprlloaapccekkrsslyddoocchcuaumrmaecenttneatratitiizooennd ooannnd the. sspmrttueoudptdhoyyortlldaiaococlknkossgiynaa.nntahalTelyyhtsteiiocclaasulatl imccooonpnsnlf.efiirrpmmuaarttitiioyonnwooaffstthnheoetaapmmroooupunentrtlyooffcfhflluauoroarrocotcechhreiezmmeidiccaaallnd the
proportion in the solutions.
RREEFFEERREENNCCEESS
TTPahhuiilss, ssMttuuNdd.yy 1ww9aa7s5s.ccoonndduucctteedd bbyy 33MM CCoommppaannyy,, EEnnvviirroonnmmeennttaall LLaabboorraattoorryy., SSt.t.
Paul, MN, 1975.
omOLaMTsHteEcRRhanged: 6.28/00 Lastchanged:6/28/00
0000000
11111199..00000022
33MM_MMNNo0t166s5B8221122
J oe740101
DVpHOrsiOTsAL DONEC Loe ----------
gl TTroexeoTnegsetnim,9B6l-eHaaul Static enAY Ek wa. wien MaterialTested. SL Fe-con md,
Time Started
D L090 Lr
Plotted LCgq
otueton ec Cte Ciltet citywafer
wn
"mn
Lepomis puctochirus
a
Pr
cone. survbal ph pin survivei ph Dn sunbar pi be sinter pu Up
eel _pog[7]70]100Tales]loo Jeol6]\0o ri]sd
\scowal (00[72]65]100 T12le=] Gp [to]ss| $0fralsd
00" ion[tal ea] too fr2lea| 70[sq]sofon)
quo 100 ts go]Solz5]5s]ao[reso] 20feslad
2a] 40d]20 sfag]0Jisfad 0|+]
400 oles 0 | [ 0 J+To [oF]
Average Temparsturg
=
i
a Bluey!
wHTEeo RE r I00 ._I I.i! I~ !l
io: i
Ed
L~SO ~alculation
To
rCaHe Th fiai T !2 _2_::.; " : "
ts
Sa
Poo
L~
; Cl
~ - | [DJoth | ji es 1Y0o0 ow wo30 4a0e e SO e6r0 700 8800 Ts~0101000 + soit Survival
11111199..00000033
3M MN01658213
Le
ENVIRONMENTAL
ENVIRONMENTAL
ENGINEERING
ENGINEERING
LLAABBOORRAATTOORRYY
AAQQUUAATTIICC TTOOXXIICCIITTYY -- PPRROOBBIITT AANNAALLYYSSIISS WWOORRKK SSHHEEEETT
DoawteesuSrtaerdtie_d_: g~-o~n5c--o?oJrTine Startedi__2:00 (PW)
MNaatteerriiaall TTeesstteedd::
600
Test Organise(Fathomainnew
Type
Type
Test:
Test:
((CoC nt~ im} om(S imt)atSitac ti~ c)
Ave. Nt._s
(gram)
Exposure Period:
96--tour _ Avavtge. CWotn.plo,%ted:(gram)
AAvvgg.. SSiizeze. (inch -- 295 (inch~
DEixlpuoesnutl:e(cPaerrbioond.: FILE. -SE.P~. --CH itoyuWrer.) ADnaatleysCiosmbpyl.eted:
Diluent:~carbon-filt.-St.P. City Wtr.) Analysis by: ~,~..
w (1)
No.
oKxf
Doses
boeed No. of Doses
fone. |
OOwbbossreecrravvleeiddey ~4||
@ (2)
MEEox~rpteeacclttieetddy |~3]
(3)
CCoorrVrrieetccutteeesdd|
O 0th-s-e&rEved-|| EOxbpseecrtveedd-|
CCtooonnctthrriiibb(uufttriiooomnn Ntoomoc.ghria(pfhroomi)
yPlotted 1
b
oo
ed
"20 T /3 T-- 1 Mortality ~rtalit~
Values
7T Expected
043| Nomograph #I
.oq
2 5
loool oq oe[| Fo
mo 1 78 TT 2 | o 37 |
s4 S
[ --oO
1TT o. /,50
[rT7r
6 7
7
[ 11
Ke
K
4 misals/boses
=
Animals/Dose
50
~0
TToottaall ccoonnttrriibbuuttiioonn ttoo cchhii
0.930
Tots
Total
A AnniimmalIss
==
RO
fai? mos) for 00d) ~O_
(@iD)e
{chi2)=
contrib.
contrib,
to
to
chi
chi
x~
TToott..
AAnniimmaallss
2 degree of freedom (froaTavie 2
= App == _590
chi2 (P-.0S) for (K-2)
~
heterogenAousb. o is tess than Z~o~C>
is less than
S09 ; degree
~.~ ;
therefore, of freedom
therefore,
the data are not {fromKTable 2)
the data are not
=
ssiiggnniiffiiccaannttllyy
heterogenous.
995)%~ Confidence ine for tgs
sons
Lg, +
ausongll wi,- Confidence zon) Limits for 1,
[e350 py)
Stope
Slope
function
f~ction
({5s))
=
/1[-gy/LCs~+ LCgo/LC,,. = sisofloe 7/,
s
eo/wSo +14% \AT = \.42
TToottaall nnuummbbeerr ooff aanniimmaallss
: a flcg, = (FTA
fLCs0= {S)2"77/ ~"
uus~eedd =
=
2
bbeettwweeeennb1v6% aanndd 8844%% eexxppeecctteedd eeffffeeccttss
(\a o~Xg ~Lk. ~= (f(rform om NNoommooggrraapphh #2;
2
((NN'))==
1LLeecgggy/=XfLiCCg,= +lo\uvpeSproerloimliitmit= \=S\oSoe/o1(x9\5X1% Lc=on=fidenceNALSimits 2 ra LCso =
k~OV
(95~ confidence limits
40
A155)
(1)
(i)
CDDooonsnnoeotctutl1iiivsstte
mm1oo0rr0ee8
ettfhhfaaennctttswv.oo
ccoonnsseeccuuttiivvee
00%%
eeffffeeccttss
oorr
msoorree
tthhaann
ttwoo
(2)
(2)
Expected value for any dose consecutive I00~ effects.
Expected value for any dose
sshhoouulldd
bbee
ggrreeaatteerr
tthhaann
00..0011%%
oorr
lleessss
tthhaann
9999..9995%%..
* There is 3 95% chance chat the true value of LC, Lies within these linits. 7 ((33)) CCoorrrreecctteedd vvaalluuee ffoorr eeaacchh 0O%~ oorr 110000% eeffffeecctt ((ffrroomm TTaabbllee 11)).. * There is a 95% chance that the true value of LC50 lies within these limits.
11111199..00000044
M3M_MNMONt061s65B82211s4
TET TTT IL!L
Terr TT
mE EN TEoRn E ee
a % eT soEELL EEE EERE
E alE l
LE pel
HA|loomctad vEaRlsE]!
T B he ome men E Er rE meTeHe Te
= HL Lh
woe
HE le
TOR
enc. walle
.
3M_MN01658215
1119.0005
