Document bOLE5rRYb90MjgNddRdZRLZ2Z

gopy __ COPY HYPERKERATOSIS INVESTIGATIONS Georgia Coastal Plain Experiment Station and United States Bureau of Animal Industry Cooperating August-Deeember 1952 At the Knoxville meeting we proposed to test chlorinated diphenyls, creosotes suspected of causing trouble, greases of the type used in the Wisconsin pellets machine and to continue study of the fractions from the petroleum cracking process in cooperation with the National Cancer Institute. The following Is an account of our work In the three month period after the Knoxville meeting. We terminated our project on December 1, 1952. Chlorinated diphenyls Samples of Aroclor 1042*and lo6b*were obtained through the courtesy of the Monsanto Chemical Company and fed to calves mixed with peanut oil. Ten doaes, each containing 0.05 grams of the compound were given on successive days, Sunday excluded. . This total dose of 0.5 gram produced no symptoms and was repeated with a dally dose of 0.5 grams. This total done of 5.0 grams produced no symptoms and was repeated using 5*0 grams as the dally doae. This total dose of 50.0 grama also failed to produce symptoms and this phase of the study was concluded. Plasma vitamin A levels remained in the normal range during and after the trials. Creosote trial Only one specimen of creosote that was suspected of being Involved In a field case (Van else) was available for study. This was fed to a calf at the rate of 10 cc per day mixed with an equal quantity of peanut oil for a total of 78 days excluding Sundays, or 670 co of creosote. No symptoms developed. Plasma vitamin A levels remained In the normal range. Presses and Oils An extrema pressure (EP) grease (Texaco EP l) made by the company which manufactured the lubricants for the machines used to make the pellets Involved in the Wlsoonsln outbreaks was fed to a calf. Ten grams per day was given In a gelatin capsulj for 112 successive days exclusive of Sundays, for a total of 960 grams of grease. No symptoms developed. Plasma vitamin A levels remained in the normal range. An EP oil, Texaco EP 90 was fed to a calf in a gelatin capsule at the rate of 15 00 per day, exclusive of Sundays until 055 cu had been administered The calf remained normal. Petroleum Cracking Process Practlons It was desired to study the carcinogenic and hyperkeratogenlc properties of two fractions. One of these called clarified slurry oil is used wrtoi'srence to Aroclor 1049 "aridlObS is, undoubtedly, an error and should h?.v? been Aroclor 1242 and 1268 P.G.B MONS 096779 COPY COPY ) principally "to reduce the vlscoelty of the asphaltic resldlum obtained in the distillation of the orude (petroleum) to an acceptable level for use in oil burners. (Steam generation on ocean-going vessels and in steam and power generation In the large boiler Installations in industrial plants and power houses.)" The first oalf (1*3^) which was given topical or local applications of slurry oil by paintings three times a week for 66 days, was reported at the Knoxville meeting. There was hyperkeratosis and thickening of the skin as found by clinical and post-mprtea examinations. The previously unreported hlstopathologioal findings of two pathologists In different laboratories are attached for calf 1*34. . Another calf (1>3&) was painted on an area 6" x 8" in diameter with slurry oil three times per week. Skin thickening was evident In four days and progressed to a very marked hyperkeratosis probably Indistinguishable ollnloally from aturally occurring cases of X-dlsease. Painting was stopped several times during the 141 days between the start of the trial and the death of the animal. Fifty-one applications were made during this time. Symptoms of depression, diarrhea, anorhexla, lachrymatlon, and weakness were evident at the time the painting was stopped and prior to death. Autopsy lesions inoluded elevations on the mucosa .of the esophagus, very extensive hyperkeratosis of the skin, reddening of the mucosa of the abomasum and small intestine and a flbrotlo liver. There were no muous cysts or other lesions noted. Plasma vitamin A at death was 22.8 gamma per 100 milliliter. The hlstopathologlcal findings are attaohed. A calf painted regularly with bunker C fuel developed extensive hyperkera tosis but no other symptoms or lesions. Plasma vitamin A remained in the high-normal range. The first calf given slurry oil orally was reported at the Knoxville meet ing. A second calf, (342), was given a dally oral dose of 5 cc of slurry oil In an equal amount of peanut oil for a total of 3^5 cc of slurry oil. This was administered over a period of 81 days. It was necessary to stop doseage several times when the animal became depressed, went off feed and laohrymated excessively. There were no proliferations or other lesions suggestive of X-dlsease. The only lesions suggestive of oarclnoma were bllster-llke elevations of the wall of the rumen. Some appeared to contain a fluid whereas others were more firm. There was an ulcer at the pyloric orlfloe In the mucosa of the abomasum. The hlstopathologioal findings are attached. HONS 096780