Document bBrY6rxDMGqDKmRnBExdx4bwD

CSHapI.J^_y.t.?rSr:^ r : . * AR226-2870 Copies to : Material Tested E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Vedicine HASKELL LABORATORY REPORT NO. 144-78 Haske11 No. II,921 Sample Ready for Testing 1/6/78 Other Codes Material Submitted by Chemicals, Dyes and Pigments, Chambers Works Company Sanitized. Does not contain t r c a rot Procedure: A range finding test was conducted with the test material to determine a rio to minimally irritating concentra tion. After this concentration was determined, the test for primary irritation was conducted by applyi'g, and lightly rubbing in, I drop (-- 0.05 mil of a 100$ and 10$ suspension (vol./vol.l of the test material in acetone cn the shaved intact shoulder skin of 10 male albino guinea pigs. To test for the sensitization potential, a series of four sacral intradermal injections was given, one each week over a three-week period, which consisted of 0.1 ml of a 1$ soluticnivol./vol.l of test material in dimethyl phthalate. Following a two-week rest period, the test animals were challenged for sensitization by applying, and lightly rubbing in, I drop (-0.05 ml 1 of a 100$ and 10$ suspension (vol./vol.l of test material in acetone on the shaved intact shoulder skin. A group of 10 previously unexposed guinea pigs received similar applications at tne time of challenge to provide a direct comparison of the chal lenge reactions -on skin of similar age._________________ Results: ______________________Reactions* cn Intact Gu 1nee Piq Skin_____ ____________________ Test Areas iAnimals - In. Avg. Wt. = 490 g .(10 `Jew Animals - Average = 642 Fi na 1 Avg. Wt. = 645 gl Concentretion in ace+one 100$ 10$ 100$ .0$ Primar2y4 HIrcruirstation Test 48 Peurs C s iIer.ge "Test 24 -n u rs "V:rs S e -s it iz e t io n Response Ratio** l+, 9 neg. 10 neg. 2+, 6 neg. 10 r.eg. 0/10 10 neg. 10 neg. 10 neg. 10 neg. -, 9 neg. 10 neg. IC re. IC nee. w 2- - S4m"iarY : produced slight to no irrita: ion in 24 hours and no irritation at 48 hnnr-c k + * J on ,he shaved intact skin of guinea pigs. As a 10$ suspension, no irritation was o b s e r v e d ^ A t r h u +eS d 3S received animals showed a sensitization response. No dermatitis hazard is exoec+e- frnm -h h cnal,ene * nne of the : ;st industrial hygiene practices are followed. expecte. from .ne h a n d m g of this material if good Company Sanitized. Does not contain t s o a r w Reaction Cods: t. **. ttt - lid, derate, strong erythema; tt+t . erythema plus e d o ; ***** . necrosis. applied initial fy^or'o^the concuirlnt^Introis. '"CreaSa a+ Cf,al lense over 1hs response expected tree, the seme amount DrE :Idt = = i'.r f ;c . 144-75 issued: March 3 I, 1978 Report by: / A u * J' Doris F. Edwards To xico lo g ist Approved by: _ Neil Krivanek Chief, Dermal and Ocular Toxicology S :t i on I'Mi'll i.irLj .j,- y - , , . ...y ... . ....... ,