Document bBmoapeb0OznDZEdG01vJ6Lko
MAY,3.ZUUU11:57AM ENVIRONMENTAL2,L3AEB09
NO.1926 P. 2
Tabl2e:
later-AsPsraeycisiaonndAccuracfyorPFOS QualitCyontrol SampleIsnRatSomniforStudy#FACT-TOX-111
Run No. QCI 0.2
PFOSConcentra(taito/nmL)
QC2
QC3
QC4
QC4
6
is
100
100
(1in 0 Dilution@)lin20 Dilutiony
1
0.232 6.17 18.2
NA
NA
0.212 6.28 18.4
NA
NA
0.210 6.11 18.2
NA
NA
0.216 5.97 18.2
NA
NA
2
0.216 6.00 18.1
NA
NA
0.227 6.09 19.0
NA
NA
0'224 6.07 19.8
NA
NA
0.219 6.18 18.5
NA
NA
3
0.211 5.97 18.8
116
NA
0.210 5.84 19.2
114
NA
0.217 6.07 19.8
113
NA
0.214 5.99 17.9
106
NA
4
0.222 6.49 19.3
NA
112
0.233 6.23 19.7
NA
109
0,214 6.S4 19.6
NA
117
0.225 6.30 18.5
NA
113
Mean
0.219 6.14 18.7
112
113
RSD (0/o) 3.43 3.08 3.28
3.61
2.78
RE
9.39 2.38 3.86
12.3
12.7
RSD - (SD/Mean)x 100%
RE - [(Mom-Nominal)/Nomir4xl]100%
NA: Not appficable.
VANCED BIC)ANALYTICAL SERVICES. INC.
Pap 10 of19
99ADJA03.Mi.DOC
m <
In, 0
m
Table 3:
later-AssayPrecision&Rd Accuracy forPFOS CalibratioiSatandards in Rat Serum forStudy MACT-TOX-111
Run No. STDI STD2
0.05
0.1
1
0.0496 0.0993
0513 0.101
2
0.0470 0.101
0.0538 0.101
3
0.0490 0.105
0.0511 0.0980
4
0.0522 0.0950
0.0491 0.104
Mean
0.0504
RSD (0/o) 4.24
0.100 3.04
RE 1 0.775 -0.425
RSb = (SD/Meam) x IOOP/a
RE= ((Mean-NominalyNominal]
STD3 0.25 0.239 0.243 0.244 0.244 0.254 0.242 0.246 0.246 0.245 1.80 -2.16
x 100%
PFOS Concentrafion
STD4
SID5
STD6
0.5
1
2
0.493 0.957
2.13
0.491 0.%3
2.12
0.542 0.899
2.03
0.539 0.928
2.03
0.482 0.980
2.15
0.487 0-937
2.01
0.484 0.933
2.11
0.481 0.958
2.13
0.500 0.947
2.09
5.08
2.66
2.67
1 -0.0325 -5.31
4A9
L) STD7
4 4.39 4.38 4.30 4.43 4.49 4-39 4.47 4.50 4.42 1.52 10.5
STD8 8
7,72 7.76 7.67 7.77 7.80 7.39 7.59 7.89 7.70 1.99 -3.76
STD9 16 16.0 15.5 16.0 15.8 16.9 15.8 15.9 15.3 15.9 2.98
-0.651
pw itof19
MAY. 3.2000 11:57AM ENVIRONMENTAL LAB 2 3E 09
NO.1926 P. 4
Table 4:
CalibrationCurve StatisticfsortheDeterminationofPFOS InRat Serum forStudy #FACT-TOX-1 II
Run No. Quadmtic
Linou
Coefficie(nAt) Coefficie(nBt)
1
-0.0004154
0.1025
2
-0.00OS419
0.1001
3
-0,0006070
0.1046
4
-0.0002665
0.1036
Mean
-0.0004577
0.1027
y A)e + Bx + C, weishted1/?
Intercept (C)
-0.0006090 -0-0004172 -0-0001313 -0.0004533 .0.0004027
Coefficienotf Determination
0.9977 0.9959 0.9968, 0.9966 0.9968
AWOVANCEE)
BIOAP4ALYTIC.AL
SERVICES.
INC.
Page 12 of 19
99ADIA03-Ml.'DOC
Table 5: Concentratims of PFOS in Rat Serum Samples from Study NFACT-TOX-111 Folkwing the0
m71 Smnpling
PFOS Concentratio(npgtml)
< tl zf) mm
Time (day)
13726 13726P 13727
13727P
Rat Number* 13728-
1372$P
1373
20
0
0.215 NS 0.121
NS
0.0930
NS
0.08
p
7
0.176 NS 0.0835
NS
0.0711
NS
0.08
14
O.Ogn NS 0.0638
NS
0.0530
NS
NS
15
0.168 NS 0.0932
NS
0.0605
NS
0.061
21
0.0849 0-0712 0.0587 <,LQ(OtO452]<(0.05<)LLQCO.0395]<(0.05)<LLQ[0.0343]<(0.05) NS
22
0.0597 NS 0.0517
NS
<LLQ[0.0431]<(0.05)
NS
NS
*RatNumber witha "P' indicatepsup sample.
<LLQ - Lem thandw lamer Limitof Quantitation(0.05iLghnL).
NS: No sunple.
Pop 13 of 19
m F)u
< Z@ @ r) M ro So o-
pr
Table 5:
(Contmued) Concentradons ofPFOS inRat Serum Sompla from Study #FACT-TOX-1 11 Fol 0 mg/kg Dose
Sampling Time
PFOS Concentratio(nAgtmL) Rat Nwnber*
(day)
13734P
13735
13735P
13736
137
0
NS
0.0947
NS
0.113
N.'
7
NS
0.0700
NS
0.108
N.'
14
NS
<UQ[0.0492]-qO.05)
NS
<LLQ[0.0474]AO.05)
N.
15
NS
0.0574
m
0.0693
N.
21 <LLQ[0.04091<(0-05<)LLQIO.0418]<(0.05<)LLQ[0.0401]<(0.05)<LLQ[0.0491]<(0.05<)LLQ[0.04
22 .
NS
<LLQ[0.0370]<(0.05)
NS
0.0592
N,
-I
*RatNumber with a 'P' bmhcatespup sample.
<LLQ = Les thantheLower LimitofQuantitatio(n0.05pg/mL). NS: No swnple.
Page 14 of 19
m m mV <
r) n
mm
p
Tabk 6: ConcentratiofnPsFOS inRatSerumSampleskom Study#FACT-TOX-1II Followintghe0.1
Sunphng Time (day)
13739
13739P
13740
PFOS Concentratio(nPgfmL) RatNumbcr*
13740P 13741 13741P t3744 13744P
13745
13745P 13746
0
9.78 NS
12.1
NS
11.6 NS
9.89 NS
9.8i NS
11.1
7
9.50 NS
7.85
NS
7.59 NS
9.18 NS
7.40 NS
8.49
14
1.82 NS
1.99 NS
1.51 ms
1.96 NS
2.55 NS
2.03
15
5.88
NS
7.83 NS
6.67 NS
6.04 NS
5.90 NS 6-65
21
1.81 2.34
4.00 2.12 2.69 1.98 3.04 2.29 2.34 1.88 2.84
22
1.33 NS
1.47 NS
1.19 NS
0.992 NS
1.09 NS 0.945
*Rat Number with a "Pn indicatepsup sunple. NS: No sample.
Page15of 19
Table6: (CoatimitCeodn)eemiratiofnPsFOS inRatSernmSamplesfromStedy#FACT-TOX-111Fol
0.1mg&g Dose
m
31
<
Sainpling
PFOS Concoitratio(nILg/mL)
nm
Time
RatNumbff*
(a
(4ay) 13748 13748P
13749
13749P
zL
OP
0
10.2
NS
10.7
NS
7
NS
NS
8.61
NS
14
1.35
m
Is
536
NS
1.87
NS
9.40
NS
21
3.94
1.74
3.27
2.07
22
0.942
NS
1.22
NS
I
*Rat Nmnber witha'?" indicatespup sample.
NS: No saniple.
Page16of 19
MM> m C)0 M 1> <i@
nm r-0
m !010
2n >
Pr
Table 7: Concentra(ionsofPFOS inRat Serum Samples from Study #FACT-TOX-111 Followingthe 1.6
Sampling Time
(day)
13751
0
155
7
149
14
21.2
15
95.6
21
38.5
22
17.1
13751P
NS NS m NS 35.0 NS
13752 13752P
164
NS
169
NS
24.1 NS
109
NS
34.8 33.9.
19.5 NS
PFOS Concentratio(npg/mL) Rat Numba*
13753 13753P 13754 13754P
213
NS
198
NS
157 NS
152
NS
21.6 NS
21.2 NS
tl2
NS
103
NS
32-6 28.3 37.1 23.4
18.2 NS
9.49 NS
13755
f 19i 138 NS 125 112 NS
13756
199 158 31.0 log 40.4 19.9
13756P
NS NS NS NS 47.4 NS
*Rat Nut&er with a "P" indicatepsup sample. NS: No swnple.
Pap 17of 19
9
31 0< z
m p 20
Table7:
(ContinueCdo)ncentra#oonfsPFOS inRatSerumSamplesfromStudyIIFACT-TOX-11F1ol 1.6mg/kg Dose
Sunpling Time (day)
PFOS ConcentFatio(nPghnL)
Rat Nunibcr*
13759P
13759
13759P
0
NS
198
Ng
7
NS
133
NS
14
NS
20.4
NS
Is
NS
99.4
NS
21
25.6
33.0
26.3
22
NS
15.4
NS
*RatNumber witha*r indicatepsup sunple. NS: No swnple.
Page Isof 19
m 31 < < ol mqm
20 p
Table 9: RepeatAnalysisof Rat Serum Samples from Study #FACT-TOX-1 11
Rat Treatment Time Number
Original Conc. jigimi
Onging
Curve Number
13753
3 Day 0 >ULQ-222.9138>(200) 3
Reason for
Remay
1
Rcomy Conc. pglml
213
>ULQ: GreaterthantheUpper LmnitofQuantitatiorL REASONS FOR RFASSAY t).Run 3 conccntratieoxnceeded calibratiro=nW. REASONS FOR REPORTED CONC: 1).Run 4 concentratiownithincalibratinomnr.
Reamy Curve Number
Report Conc pg/m
4
213
P*p liOpf19