Document bBORoOVLkBYQwEEpJO3NJELG3
MICROBICS MICROTOXO TOXICITY TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component of FC-602. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 501. The testsample isFC602. Currentinformationindicatesitisa mixtureof 1% PFOS, 12% diethyleneglycolbutylether,61.05% water,20% ethyleneglycol,2% Sultone foamer, 1% sodium octylsulfate1,% sodium decyl sulfate1,% magnesium sulfate0,.7% xanthan gum, 0.1% starch,0.1% n-(3-chloroailyl)hexaminium chloridea,nd 0.05% tolytriazole.
The followingsummary applies toa mixture with incompletely characterizedconcentrations of impudties. Data may not accurately reflectthe toxicityof the tluoruchomical component of the testsample.
METHOD:
Method: MicrobicsMicrotoxV"BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1988 Species: Photobacteriumphosphoreum Analyticalmonitoring:pH and osmoticcondition. Exposure period: 15-minutes Statisticamlethods: EC50 valuescalculatebdy statistilcianlearegression program providedforMicrotoxC Test organism source: MicrobicsR'eagent. Test conditions:
Diluent:Microbic'2s% NaCl Reagent Test temperature:Not noted. Stock and testsolutionpreparation:A primarystocksolutiownas preparedby dissolving200 mg oftestsubstance in100 mL ofMillipore Milli-Q'w"ater. The pH ofthe stocksolutionwas 5.5and adjustedto 6.7. An osmoticadjustmentwas made using200 mg NaCi dissolvedin 10 mL stocksolution.Appearance oftestsolutionswas noted as "apparentlycompletelymiscibleinDI water,no colorapparent".All testsolutionswere made by proportionadlilutions. Exposure vessels: 4 mL cuveftes. Number of replicates:two Number of concentrations: Four plusa blankcontrol Element basis: Percentlightloss
RESULTS
Nominal concentrations: Blank control,125, 250, 500, and 1000 mg/L Element value: 5-minuteEC50 = 210 (190 - 230) mg/L
15-minuteEC5o = 220 (200- 230) mg/L
Element valuesbased on nominalconcentrations.
Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks fieldT.he valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The FC-602 15-minute EC50 forPhotobacteriumphosphoreum was determined tobe 220 mg/L witha 95% confidenceintervaolf200 to230 mg/L.
Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,MN, 55133
DATA QUALITY
ReliabilityK:limischranking2. Testingmeets allcriterifaorqualitytesting. However, sample puritywas notpropedy characterizedand the studylacks analyticaclonfirmationofthe amount offluorochemicalproportioninthe solution.
REFERENCES
Thisstudywas conducted by the 3M EnvironmentalLaboratory,St.Paul, Minnesota,Lab Request number Fl 151, 1988.
OTHER
Last changed: 6/26/00.
LR ENVIRONMENTAL LABORATORY MICROBICS MICROTOXR SYSTEM
RAW DATA
TEST SUBSTANCE: FC - to0 a I-
TEST DATE-
9.51
ANALYST:
1-1)n+@e-r- Arc- PIU5 -tyi -Q400 -Aall -S? L,,+
SAMPLE CUVETTE
Bl
ci
B2 C2
FINAL ASSAY CONFENTRATION rn (4- I L u-
BLANK CONTROL DUPLICATE
bu plI'c&i e-
I N ITTA-L-READING 1(0)
FINAL READINGS
5-MIN. 15-MIN. 30-MIN. 1(5) 1(15) 1(30)
0
7
73
crc) 0
so
12"2,
3
Li
'50
83
C3
bu -pi
CA 7
X/C)
lo,
37
3
B4
Cl 9
A4
I
C4
lbu plic&ie
q1
7
85
/ooo
140
16-
C5
't) UPI Icc,+ e@
Test
\'%Scous.lorawv%tt'bv%ca
Siocv SOIL i
A 00 (vt 100 r@& bl- LuOe-r, Eqclv IrlUz 2 1-n
4pporehvlyeom'Ple.+-e-ly rni6ci'61cin ol: u-,aiern,o ftior appar@P-1.
r
St-OCtC SCin. ptfZS.,57 -441vs@ek-+o 6-7 wi+kcti'iu+e-
A/a-60
osvvtoitc,exc.a@v-s-+etni4i - AzU-ed-;tcorn@,),4ct io iorme s+oc goill
Solu+loy@
DI:
dL6(.)V'e.
wer<_
DSSISY COrLCenifddiM-
6P rO%-u t P-oujA) OLh
mLcidLie-
JOU4.)er roLU tPOUL)L. B
L
]-;tq.@gg A4erd-+Ior\si-ari-e@L )-;L,7-.@e) pi4 6, S?
MICROBICS MIMarOX R SYSTIN MICROTOX R DATA REDUCTION
3LANK RATIOS*
Final Initial
I
5-MIN.
15-MIN.
ILANK CUVETTE
IRLANK RATTO InTFFERENrP
K RATTA DTFFERFNCP
BI
r6 h -:o.pqql
11
ci
83 //a,z>o .1.3a C)-C)
qhe
blank
ratios
for
BI
and
Cl
should
17 agree Within'--0.02.
LR Date 1'e Analys
us
BLANK RATT
DTVFFRVNry
IEAN BLANK RATIOS
Bl BK RATIO + Cl BK RATIO 2
57M-IN. t,- RA 7
15-MTN8 e)t-3
IN-MTM-
-
IORMALIZED LIGHT LOSS (PEFFECT)
Mean Blank Ratio x Initial Sample Readinx Final Sample Reading
;.AMPLE CUVME est Concentration 12 '2 13 '3
'4 35 15
5-MIN. 6. 59@ c-707
7 1 79
15-MIN. 0. 571l
0 -L-7 1.0057
30-MIN. rT
LIGHT LOSS
x 100
;AMPLE CUVETTE
rest Concentration
32
rnZA)L
C2 );45-
B3
C3
B ^--4
B5 @@5 /o
EFFECTIVE CONCENTRATIONS
5-MIN. Z a 37
41 51 57
b7
81 Ig I
(EC"s
L
15-MIN. %a 3 @
30-MIN. 2 A
7/ lo '7 I 9- 1 I
(calculated using "P.C" data pairs.)
EC@S
5-MIN.
ec20
EC50 (95Z Confid.)Limits)
C) Iqo- A 3o@
15-MIN. c. on - 0)
30-MIN.
Se-@p-p A -I- -@-0c- G+oc V- -@est- So tvi- rep: