Document bBG253KR2KBRzeZ27gzxk6x7y

TO XIC ITY TO AQUATIC PLANTS TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603F. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 501. The test sample is FC603F. Current information indicates it is a mixture of 0.5% PFOS, 7% diethylene glycol butyl ether, 82.95% water, 2% acrylic foamer, 4% Alkylether sulfate, 1.45% Xanthan gum, 1.4% calcium acetate, 0.07% N-(3Chloroallyl)hexaminium chloride, 0.5% triethanolamine, 0.05% sodium hydroxide, 0.03% cis-1-(3-Chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: OECD Guideline 201, and EPA C FR 40 797.1050 Type: Acute static GLP: No Year study perform ed: 1995 Species: Selenastrum capricomutum Source: In-house cultures. Originally from ATCC, Rockville, MD Elem ent basis: Algal cell counts (cells/mL) and growth rate. Exposure period: 96 hours S tatistical Methods: Not applicable due to study results. A nalytical m onitoring: Not given Test Conditions: Algal N utrient Medium : Nutrient medium for freshwater algae per OECD method. This nutrient medium served as the diluent for all algal operations. The pH of this synthetic algal medium was 7.9. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared by diluting 500 mg of test substance in 500 mL of standard algal nutrient medium. Aliquots were then added directly to dilution water in ' test vessels to create test solutions. Exposure vessels: Not given Agitation: Not given Num ber o f replicates: Not given. Initial cell loading: 1 x 104 cells/mL 002453 Num ber off concentrations: one plus a blank control Lighting: Continuous. W ater Chem istry: Not given RESULTS_____________________________ ____ Nom inal concentrations: Blank control and 1000 mg/L 96-Hour Algal Growth Response Exposure Cone. (m g/L) Control 1000 Mean Growth Rate 1.348 0.896 Percent Change in Growth Rate -- 34 Elem ent values: 96-hour IC50 = >1000 mg/L Element values are based on nominal concentrations. R eversibility off Growth Inhibition: Not given. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS________________________________________ __________ FC-603F exhibits a 96-hour IC50 of >1000 mg/L. This concentration inhibited the algal growth rate by 34%. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Kiimisch ranking = 4. Not all laboratory data could be located. Sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number M1655, 1995. OTHER______________________________'____________________________ 002454 Last changed: 6/26/00. 002455 BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY PREPARATIONWORKSHEET FOR FRESHWATER ALGA GROWTH INHIBITION TEST" LR SEE BELOW PAGE OF d a t c io A J*S ANcTS^^r-5 VilL^ yc. Ign'AT' Lfrt *SO\ L-iflVrl- Avuhrt* --^Soluble inyUfVhsC__________ ,SQOm/i/F\OOrr>L,,y H . 'Al/yH M.ichrWn- Mgdlum.____________________ _________Ir tr fa r ^ H "1iQ._________ ____ ______________________________ ________ _ kf*'lft<P> FC.1*^ ^ Li^Wf ui/tlryr^Vfwvj dPFF. C lcair fM ih e jr ejiXnrai t.'/jmVL MA-^hL./iiOaW /ynwtL^JSki. ragal kfatelgrtf rledkuvi_______________________ .. - ^ VI *~1 ----- -- -- - F(1^>S-S<V( L^-mZPt) ~?Vri-h*/4i^<r Clnam ifA l. CXuJHi+g rn m lftc M u3t*4-h ui'M i-Arr- ___in f/U-h-vC_*prLTnrW| ^ V l. -UnAl KSlHW-mf- ___ IriH iftl `g.Q ------------------------------------------------------------------------------------- N'S Z S l__ rg ^ o i-A F Awtvy^.\irm^rt liquid. Mi&cihlg. tnutfldrT-. ftW k /-*a v w L > ^rVrl 4SAkjgkt4: f H- J m I fc#'--" &'*>- XL> n-f Jfrw n d a n j -A lry il K$LfcVWgtL-f \A M X^\aa ldag> TpyTr-p^gvij 2J ln?4Mt jpAor W pH l.q cATa-tinM, Trvi>nat^4ioV; a mrl r^ummM^x,'^ t k* tT-ftw* * . t ^ j2 > tin k i S r4 ,'ir'.; 1 C-^ir; .Jv*A% tlAOTlJLlaVVt ; r^ ll?s uf*>r\ liltxrr.-CVniAA * i Ha ii Piil-kiyy> 4-h?H- ua=><.-fh^ a A o r U K U a P. j - ICgIVes/w-1; ; S ,^ > =lfl??g i '.ec-.^V'V, - v*sr'- - -** 5 Hr, \whUaP^ BV< CfvHir&V OflurvV-> !t xift^rvllf/u i ' Test methodology conforms wilh the following two test p ro to c o ls 'll^ ^ OECD #201 "Alga.'Growth Inhibition Test* and Fed. Reg: Vol. 40/No:797.1050:M lgal^AgitgTo^citvlTes^ 'i*t-j&ifr liksc-i(&% 002456 BEST COPY AVAILABLE NUTRIENT ENVIRONMENTAL LABORATORY STANDARD NUTRIENT MEDIUM FOR FRESHWATER ALGAE CONCENTRATION IN STOCK SOLUTION FINAL CONCENTRATION IN TEST SOLUTION LR M l 655 N3188 N3194 N3281 STOCK SOLUTION 1: MACRONUTRIENTS n h 4ci MgClj 6H20 C aC lj^H jO MgSO 7H20 kh2po 4 1.5 g/L 1.2 g/L 1.8 g/L 1-5 g/L 0.16 g/L 15 mg/L 12 mg/L 18 mg/L 15 mg/L 1.6 mg/L STOCK SOLUTION 2: Fe-EDTA FeClj 6HjO NajEDTA 2H20 80 mg/L 100 mg/L 80 ug/L 100 ug/L STOCK SOLUTION 3: TRACE ELEMENTS HjBOj MnCt ` 4H20 ZnCI2 CoCfe` 6H20 CuCI 2H20 NajMo04 2H20 185 mg/L 415 mg/L 3 mg/L 1.5 mg/L 0.01 mg/L 7 mg/L STOCK SOLUTION 4: NaHC03 185 ug/L 415 ug/L 3 ug/L 1.5 ugt. 0.01 ug/L 7 ug/L S3 :''v NaHCOj 50 g/L 50 mg/L Sterilize stock solutions 1-4 by membrane filtration (0.2 urn). SOLUTION 5 :1NUTRIENT CONCEW ^ T E M f e v sr?r i 002457 R F S T C O P Y A V A IL A B L E ENVIRONMENTAL LABORATORY FRESHWATER ALGA GROWTH INHIBITION TEST SPECIFIC GROWTH RATE m ALGAL SPECIES: Freshwater Green Algae, Selenastrum capricomutum . DATE INITIATED: irt/q /g c DATE TERMINATED: |0 /ia f t < r tL.nO ; ^ Ji^ANAL' TEST SUBSTANCE CONCENTRATION mg/L 24 HRS 48 HRS MEAN OR RATE % C H A N G E" MEAN GR RATE * C H A N G E " BK CONTROL STD NUTRIENT Z.\<A 1.U48 'Ai. M1655 1000 mg/L 1.R88 5.5 1,,`LSI, Z7L N3188 1000 mg/L I.R5L 15 1.4R6 <U N3194 1000 mg/L 0.531 15 O .S R T (<?4 N3281 1000 mg/L 1.841 13 1.4H 14 DATE/ ANALYST JQlmlhi Test m ethodology conforms w ith the b lo w in g two test protocoteTj O EC D #201 "Alga. Growth Inhibition T e s r and Fed. Reg. Vol. 40, No. 797.1050, `Algal Acute Toxicity Test", Specific Growth R ate (u ) - ln N ,-ln N /t. W here N , is the m ean m easured final cell density; N , is the Initial cell density and t Is the tim e of the me; < Percent Change in Growth R ale 14 - (M ean Control Value Moan Sam ple ValuayM ean Control Value x 100. w ` ,,,,<Ut*S/* V,-nk