Document bB7da7YN9eDxYe9O4LNKOVOYy
ACUTE TOXICITY TO FISH (FATHEAD MINNOW)
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FX-60 1. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was cc 835-34. The testsample isL-9235 (FX-601).Currentinformatioinndicatesitisa mixtureof 1% PFOS, 12% diethyleneglycolbutylether,81.25% water,2% propane Sultone foamer, 1% sodium oetylsulfate1,% sodium decyl sulfate0,.1% N-(3chloroallyhie)xaminium chloride0,.8% xanthan gum, 0.8% starch,and 0.05% tolyltriazole.
The followingsummary applies toa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately retlectthe toxicityof the fluorochemical component of the testsample.
METHOD:
Method: Not given.
Type: Acute static-renewarlange-finder
GLP: No
Year completed: 1984
Species: Pimephalespr-omelas
Supplier:Commercial supplierB,rady,NE.
Analyticalmonitoring:Temperature,pH, and dissolvedoxygen.
Exposure period: 96-hours
Statisticamlethods: LC5ovaluesnotcalculatebdased on studyresults.
Test fishage: Juveniles
Length and weight: Average length 35.4 mm
Average weight 0.312g (wet)
Loading: 0.52g/L
Pretreatment:250 mg/L TetracyclinHeCI.
Test conditions:
Dilutionwater: Carbon-filterweedllwater.
Dilutionwater chemistry:
Hardness:
250 nig/Las CaC03
Alkalinity: 240 mg/L as CaC03
Conductivity: 350 pmhos/cm
Lighting:A dailyphotoperioodf16 hourslighatnd 8 hoursdarkwitha 15 to 30 minute transitiopneriodwas maintainedthroughoutthetestingperiod. Stock and testsolutionpreparation: The testmaterialwas added directly tothe dilutiownater on a weight/volumebasis. Concentrations dosing rate:Test solutionsrenewed. Stabilityof the testchemical solutions: Not noted.
Exposurevessels:1 galloanll-glarsosundtankscontaini3ngL test solution. Number of replicates:1,except forhighestconcentrationand blank controls. Number of fishper replicate:5. Number of concentrations: fiveplusa blank control Water chemistry during the study:
pH range (0-96houm): 8.0 - 8.1 (controelxposure) 7.8- 8.1 (1000mg/L exposure)
Tempomture range (0-96houm): 20 -22 OC Dissolved oxygen range (0-96houm):
6.5 - 9.4mg/L (controelxposure) 0.5 - 9.4nig/L(1000 mg/L exposure)
RESULTS
Nominal concentrations: Blank control,1,10, 100, 500, and 1000 mg/L. The blankcontroland 1000 mg/L exposure solutionswere evaluatedin duplicate.
Elementvalues: 96-hourLC5o=>1000mg/L
Element valuesbased on nominalconcentrations
Remarks: Testingwas conducted on a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsample 96-hourLC50 forfatheadminnow was determined to be >1000 rng/L,the highestconcentratiotnested.Ten percentmortalitwyas observed inthisconcentrationwhen the replicateasre viewed as combined.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking= 3. The studyisa range-findingw,ithlimited testorganisms. There isa lackofcharacterizationfthetestsample and the studylacksanalyticaclonfimiatioonfthe amount offluorochemicaplroportion inthe solutions.
REFERENCES
Thisstudywas conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number Bl 684, 1984.
OTHER Last changed: 6/26/00
ENVIRONMENTAL CHEMICAT E-NVIRONMENTAL
TOXICITY f E ST
LABORATORY EFFECTS EVALUATION
SUMMARY REPORT
Chemical Tested
est Type
LCsnYEC50/IC50/GR50
(95% C.L.
z
Test Species: Test Da te:
AW7@7U&4b
L. R.
Analyst(s):
(Exposure/Test Conditions)
mg/l
Exposure Type:
Static Static Recirculator
Static
Static/Continuous Orbital Shaking-
Renewalvilit7l!5--V--,Flow-Through
Diluent:
Aerated Well Water (Initial D.O...!5?,@mg/l; pH_15jj ; Temp.
7-
Water Hardness:
,O,"S6
ppm Hardness Classificationr-,-
OC(/,o
Dissolved Oxygen t Saturation:
48-hr
96-hr
Extended Exposure
cchtrol(s)
Ld-West Experimental
Highest
Experimental
Dose 1 Li ol@5
DosetooonLi
pff Levels,o
48-hr
96-hr
Extended Exposure
Control(s)
Lc)west Experimental
Dose lo!t@IJ
Highest Experimental
DoselOOO
4 e2
Test Temperature OC (OF):
Photoperiod:
Sixteen
hours
light
with
48-hr
96-hr
Extended Exposure
o?ZC (M. AZ@c(7,Z,4OC( )
a 15-30 minute transition period.
,.LrLAJ A.OrLL@VA%11.L VZ%4
AQUATIC TOXIC;TY DATA SHEET LR
ype Test&ve-
MaterialTested4_@42@@@@,ot
77C
est
Aug
5z64(!6'd;f
Date Rec Id
/lBatch
-
.10
-@@ /A7L
vg.Wt.& @2@ams Avg.
No. Organ3.smsper dose -'S- Ioading F'acWr 0_
9/W
ish Disease Treatment@,-@ 14f
7-
est Solution VOUna..@ 4@S/J@U-Glass Tark Tank Dimensiais:
iim Started_,&Wa2l&Date
Started
Date Ccmpleted
iluticn Water Carbm-filterecl well W;%+.er-(T@am.
D. 0.
nvest 3*-9a Full s
(95% ODnfidericeLimits)
ppnll.pH
rark NO.
Initial Conc. mg/l
Thtal
AMCXMT Added
Control
24 Hours
ThtlaltnNoo,. ppHH Dead
48 Hours
D.O. 7btal No. pH
ppm
Dead
72 Hours
96 Hours
D.O. 7btal No. PHID.O. Tbtal No. pH D.0
ppm Dead
ppm Dead
PPM
;;L- lo c.r) --erl))
Al
I/Z"
L
IrY'r)-)5(y
C/
Test Temp. OC Initials& Date
xtnients
gc
X*ndary Effects (otber than Mortality)-.-
ParLk No.
Syxnptans or Reacticns
3ntrol
24-Hr.
og-w-
I/Z
BC50
Total NUmber Affected
48-Hr.
72-Hr.
96-Hr.
141
'ov-
Fortli10620-8-8 PWO
Sample Description
Lab RequestNo.a@(ZE/4@-
.
Page
of
EnvironmentLaalb- WorkSheet
K (C t-Ty
r Date:
t-F @A
ILY-:g
Analu
7-19-84
ENVlRON-4F-NTAL LABORATORY STATUS ALL RESULTS TE.MTATIVE
REPOPT
LAP REQUEST 90. B1750
EQUESTER NA4E: ;EPT: 0222 )ROJECT NO: 36 @ATE RECEIVED: '-F.SC:MAY-CARBON
RRR
6-14-94 FILTERED
WELL WATER
CONTRACT LAB:
C09TRACT LAB COST:
0
3M E-L HOURS:
3
EST CMPLT DATE: 7-27-34
DATE COMPLETED:
-------------------------------------------------------m ------------- --------
SA.*4PLE
DATE
COD---
----------
------------
DESCRIPTION
TEST
RESULT
--------- --------------- ----------
1
6-01-34
RM#4
2
DI
WATER
PH RES-CL COND TOT-HARD TOT-ALK TS NH3-N SOL-F COD-LOW TOTMCCLIF SPC
p@q RL@'S-CL COND S?C
7.9 <0.02 MG/L 350UMHOS/CM2 240 MG/L 240 MG/L 320 MG/L 4<0.4 MG/L 0.2 MG/L 4 MG/L NEGATIVE 49 PER/ML
6.8 <0.02 MG/L 2.lUMHOS/CM2 9 PER/ML
4 = UPDATED DATA = R,ONTRACT LAB