Document bB3YXOnnXmadz0qgmn1XLMyK0

BACK TO MAIN ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE_____________________ Identity: Perfluorooctanoic acid, tetrabutylammonium salt; may also be referred to as PFOA tetrabutylammonium salt, tetrabutylammonium perfluorooctanoate, N2803-2, or as a major component of L-13492. (Octanoic acid, pentadecafluoro-, tetrabutylammonium salt, CAS # 95658-53-0) Remarks: The 3M production lot number was 2. The test sample is referred to by the testing laboratory as N2803-2. The T.R. Wilbury study number is 888-TH. The purity of the sample was not sufficiently characterized, although current information indicates it is a solution of 44.9% tetrabutylammonium perfluorooctanoate, 27.9% water, and 27.2% isopropanol. The following summary applies to the test sample as a mixture of the test substance in an isopropanol/water solution with incompletely characterized concentrations of impurities. Data may not accurately relate toxicity of the test sample with that of the test substance. METHOD________________________________________________________ Method: U S. EPA-TSCA Guideline 797.1400 Type: Acute static GLP: Yes Year completed: 1995 Species: Pimephales promelas Supplier: Aquatic Biosystems, Fort Collins, CO Analytical monitoring: DO, conductivity, pH and temperature were monitored daily. Exposure period: 96-hours Statistical methods: LC50 values calculated, when possible, by probit analysis, moving average method or binomial probability with non-linear interpolation using the computer software of C.E. Stephan. Test fish age: Not noted. Length and weight of fish in control: Average length = 34.9 mm Average weight = 0.36 g Loading: 0.24 g fish/L Pretreatment: None Test conditions: Dilution Water: Deionized water adjusted to a hardness of 40-48 mg/L as CaCO3/L BACK TO MAIN Dilution water chemistry: Not noted. Lighting: Cool-white fluorescent bulbs with an intensity of 25 ft-c. Photoperiod of 16-hours light, 8-hours dark with a 15 minute transition period. Aeration: Initiated after the 72-hour DO measurements. Stock and test solutions preparation: Test substance was added directly to the dilution water in the test vessels on a weight/volume basis. Concentrations dosing rate: Once Exposure vessels: 20 L glass aquaria containing approximately 15 L of test solution, water depth approximately 18 cm. Vessels were loosely covered during the test. Number of replicates: 2 Number of fish per replicate: 10 Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 - 96 hours): 6.3 - 8.6 mg/L (control exposure) 4.6 -8 .4 mg/L (1,000 mg/L exposure) Note: aeration was supplied to test vessels at 72hours Conductivity range (0 - 96 hours) 140 - 150 gmhos/cm (control exposure) 170 - 180 gmhos/cm (1,000 mg/L exposure) pH range (0 - 96 hours) 7.4 - 7.7 (control exposure) 7.5 - 7.7 (1,000 mg/L exposure) Test temperature range (0 - 96 hours) 21.6 - 21.9C (control exposure) 21.6 - 21.9C (1,000 mg/L exposure) RESULTS Nominal concentrations: Blank control, 160, 250, 400, 630, 1,000 mg/L. Element value and 95% confidence interval: 24-hour LC50 = >1,000 mg/L (CI not calculable) 48-hour LC50 = >1,000 mg/L (CI not calculable) 72-hour LC50 = 970 (630 - >1,000) mg/L 96-hour LC50 = 960 (830 - >1,000) mg/L 96-hour NOEC = 630 mg/L Element values were based on nominal concentrations. BACK TO MAIN Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not to the fluorochemical component alone. Cumulative percent mortality: Nominal Test Conc., mg/L 24-hours 48-hours 72-hours 96-hours Control 0 0 0 0 160 0 0 0 0 250 0 0 0 0 400 0 0 0 0 630 0 0 0 10 1,000 30 45 55 55 Lowest concentration causing 100% mortality: None Mortality of controls: None CONCLUSIONS________________________________________ The test substance 96-hour LC50 for fathead minnow was determined to be 960 mg/L with a 95% confidence interval of 830 - >1,000 mg/L. The test substance 96-hour no observed effect concentration (NOEC) was 630 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY__________________________________________________ Reliability: Klimisch ranking 2. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. REFERENCES__________________________________________________ This study was conducted at T.R. Wilbury Laboratories, Inc., Marblehead, MA, at the request of the 3M Company, Lab Request number N2803-2, 1995. OTHER Last changed: 4/01/01