Document bB3XpGq63YBK00aRk1nOpLpe3
ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA)
TEST SUBSTANCE
Identity:Perfluorooctanoiaccid,ammonium salt;may also be referredto as PFOA ammonium salt,Ammonium perfluorooctanoateP,FO, FC116,FC-126,FC-169,orFC-143. (Octanoicacid,pentadecafluoro,ammonium salt,CAS # 3825-26-1)
Remarks: The 3M productlotnumber was 37. The testsample is@C-143. It'psuritywas notsufficientclhyaractedzed,though currentinformation indicatesitisa mixtureof96.5 - 100% testsubstance and 0 - 3.5% C6, C7, and Cg pertluoroanalogue compounds.
The followingisan abbreviated summary of several previously completed Daphnia magna studies conducted to evaluate diet These studies have been superceded by a more recent test.
METHOD:
Method: Not noted. Type: Staticacute GLP: No Year completed:1982 Species: Daphnia magna Supplier:USEPA-ERL, Duluth,MN. Analyticalmonitoring:Temperature,pH and dissolvedoxygen Exposure period:48-hours Test organism age: First-Instyaorung (0.5-1.m5m) Pretreatment:Not noted. Dilutionwater:Carbon-filterweedllwater. Stock and testsolutionpreparation:Test solutionwsere createdby a common stocksolution5, g/L Concentrations dosing rate:Once Stabilityofthe testchemical solutions:Not noted Exposure vessels: 250 mL glassbeakers containing200 mL testsolution(6 cm depth). Number of replicatest:wo Number of daphnids per replicate:10 - 20 Number of concentrations:fiveplusa blankcontrol
RESULTS
Element values: 48-hourEC5o = 126 (86-183)mg/L to >1000 mg/L
Element valuesbased on nominal concentrations
CONCLUSIONS
The testsample resultsfor48-hour EC50 forDaphnia magna were inconsistent.Values generated inMay of1982 determined 48-hour EC50s to be >1000 mg/L. The value generateinJune of 1982 indicateda 48-hour EC50 of 126 mg/L witha 95% confidenceIntervaolf 86 to 183 mg/L. These inconsistenciesmay be due to differencesindiet.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking3. The method isnot described,sample purity was not propedy characterizeadnd itlacksanalyticaclonfirmatioonf test substance concentrations.The valuesfrom multipletestscontradicetach other.The purpose ofthese studieswas toevaluatedifferendtaphnid diets.
REFERENCES
These studieswere conducted by the 3M Company, Environmental Laboratory,St.Paul,MN, completed from May toJune, 1982.
OTHER
Last changed: 5/25/00
Tyre Test
ENVIRO@JME"JTA.'. LA;-TORTORY AQIJATTC TOXICITF DATA StrEET
46.- -rc ro
Mater-al Tested
Lot 7-
Test Or,-an-ism L7. indq im9t Source. -Otj/uii a(-
at- Rer'diL&yJit-.-(p*atch Nn.
Age: First-Instar yo@ng (0?5-1.Srm) No. (-,rgpnismsrer treatment _20
Test Chamber: 250ml glass beaker Test Solution Vol.: 200ml Test Solution Depth: 6cm.
Time SttLrt,-d10:W. forrV+Date Started
Date Completed
Dilution Wrter- Carbon-filteredwell water (Temg.-LIL12c, D.O.----2-PMI PH----)
Investigatc,rO.U.
i
(Full Signature)
-- (95% Confideice limits) 0
t4
Init:
nk Cone 0.
Total t
24 Hours
48 Hours
urs
gwit- Hfto
Anoun
Total
.0. Total
D.Om* TiytiI
otal
No. Dead PH ID
Added
PPM I I No.Dead pH Ipp
No.Dead LH P. rTNo.De ad
pr
Control I
0
c>
gt., B.L2
led 'Lo ,.,@a
c>
.- @ z- e> c>
Test Temp. 0C.
2.y0
Date
Commen ts:
-&AMA:&
5:@ qLA@@
Effects other than Mortality
qv
EC so
@02Y 4&
ay
j6ST!l-tVegsi 4w/
Tank No.
Control
_@ymptoms or Reactions
24-Hr.
Total No. Affected
48 Hr.
72-Hr.
96-Hr.
ENVIRO@4MENJTAA'. LA;-IORTORY AQUATTC TOXICITT DATA SF:EET
Tyre Test it8 At--ECg:6 Material Testedlr-
Lot _3*1
a@ Ckl@ Test C)roanism 17. "40L.4 "CL Source 'OU
r)at-!Rec-'dia@l&atch No.
v Age: First-Instar young (0.5-1.Svm) No. ("Irganismrser treatment to
Test Chamber: 2SDml glass beaker Test Solution Vol.: 200ml Test Sol.ution Depth: 6cm.
Time Stirted 1:W,4
Date Started SlfZZ2-
Date Completed.C/(o./tZ,
Dilution Wrter Carhon-filtered well water (Temo.
OC, D.O.@@@m,
pH___)
InvestiRatcr A-PL04-&,,
(Pull Signature)
A
Init: ank Conc
mit/I
Totalt Anotm Added
2-4 Total No. Dead
urs D.O.
pH ippm
LC (95% Confiderice Limits) so
48 Hours
Total
D.O.
No.Dead PH IPPM
;oy 43-Hours
Tot.al
(3. 1iTc;)":'tpy'Oa'in?'s
No Dead PH ;;mllNo.DeadiH
Control
30
3- Leo A?6
(>/a
1
(Plttv
*/to wo YIV
0/". si
.
ei
Yld
Y/O c -7 c 3/ifr" BL yio
tci-Y,o
11 I
Y16
0 Test Temp.
aye,
Date IIAAlyz
Comments:
fl%.
el1(
QA&AV
Effects other than Mortality
Ec so
-I
vple
4.r5H
Tank No.
Control
-@ymptoms or Reactions
24-Hr.
Total No. Affected
48 Hr.
72-Hr.
2.,dLf
6J-p2-
96-Hr.
ENVIRO@4ME"JTAYS. LA;-KORTORY AQLIATIC TOXICITT DATA SI:EET
Tyre Test
Material Tested ir'c1-11-5
Lot.-37
Test Or-anism
Source
Dat@% Recld L;LWgatch No.
Age: First-Instar young (0,5-1.Svm) No. CTganisms rer treatment 2 6
Test Chamber: 250ml glass beake-r Test Solution Vol .: 200ml Test Solution Depth: 6cm.
Timt-St irts-d If:50 Ag
Date Started
-Date Comnleted
Dilution Wrter Carbon-filtered well water (Temn._Lsif. OC, D.O.@@pm,
PH---)
Investigatc (Full Signaturoq
LC
(95% ConfideTice limits)
so
Init.
ank Conc.
mg/l
Control
io
Ioo
.roo 5' /ow
Tota Amount Added
2,0
loo
24 Total No. Dead
ours D.
PH ppo-I I
48 Hours
72 Hours
Total
D.oolif:ti
0
No.Dead pH IPP m No.Dead pH F;P;
0
a
1
Hou7s
Toottaal oNroD.Dead )H
17
t
Test T!m. OC*
oi/iy- -
TOL
-ale,
4 Date
Comments:
Effects other than Mortality
Tank 1- No.
Control
-Symptoms or Reactions
24-Hr.
EC so
Total No. Affected
48 Hr.
-94-Hr.
&MHr.
5 ri E.Ci5 I (IC,r 2 C,@ 1
C'.0r,lt,lEr-iFTG*P,---.
4
.7 24 HR PON P
LOG Ot
I ri
5 F, 1 ClClft
2 2. E.9'i---.97
FRRCTION F,E P 0 t-liiI t-f4.
0 0.05 0.25 0.85
FITTED F F-'RFI- I Dr-1
5. 72675**@E- (17 0. C,1709,'@25 0.4217381 0 . 7'-z5,5 1 4,@@
r'4D OF ITERRTIDN*---'.
INTERCEPT
7 HETEROGENIETY
-7.'612345 FACTOR (H)
LC-50
@q-@tPCT. LOWER CONF. LIMIT = '@5 PC'T. LIPPEF' C'.Fit-iLFI.MIT =
L0PE (E)
2..747:,308
t-40. OF C.Ot-iC..FOR CLIF"@EE5 IT!PUT DATFi-C-Ot-iC.@t-iD.
I Ci!2. Ci!2. 20,
(IC,9 2 0 @ Cf
FDtqt,iENTS FOR C-LIF@%@'5E 46 HR RU@l B
LOC,
1 Cl I cici
C,
CHI-SO
1 2. 2.69-S-97 PROBABILITY < 0.05
FRRC-TION F,E, :-P@:0 t-iriI t--i
0.1 0.3 1
CHI SC, FOR LINEARITY
5.Ci54567 C,
CHI
FOR
PROBRI-, I L.I TY
---0.07987569
404.5214 861.965S
FITTETI FF@F-tC.TIDr-i
0-ilSS87939 (1:5 097ESP 0. 898752*---.'
r-iD OF ITERATIONS
114TERCEPT (A)
,SLOPE
C@H I SID-FDR L INEAR I.TY
C H I Ci FOF' -PROBFIBILITY
-3.552151
1.78872.21
7.774071
0. 0(1530C'149
HETEF-,OC,ENTETI( F-FiC:TDF- (.H*,i C-.774(!71
@Ir-iC.E C,
f--,F--EFi7EFT'HF-f-i l@ THE
G LTPil!7*,--H.@F;.Etic-T I,EF I t-4ED
Lf--:-5f=l
*-;p@-,. @@ @-i19 a
F-'(-T-. LOi,,i'EF-'CEr-4F. LIMIT =
F-C:T . L,F'FE, F, C:Eit-Fi-. L I t-IIT =
C,
j
F -CFi- E;I ElF-4:: F-i'-f11F,T F-i
El.r-CL,F;%,.,E@ 5
E!F
FDF;. CL,F-.-@,,1'E
4
I
DH- TH@-C Eir-i@ t-iE,.
li
I ri..2 C, ri
11EF-ir.,
5Ciri,2(1.4
1 C,0Cl2.-C9i1
@r_,4HF,
C:D t-fi---
F--.LitH-i
I LOC-
CDt-4C
I oci
50(1
100(1
C:HI-SO
2 2.69897
3
PROBABILITtf: < 0.05
F F-F,i.C,T 10 ti
FESpot4II ING
fl.05 Oi 2
0.85
F I TTEli
FFF;C T IDr4
2. 175 tL@E-F'7
:-'03.959862
0.7177021
% @c4: -c, Lit-iliEF:FLDtRkTi LINE r-i952C,OF @iFiI r4 P F-0- G P F-r4-1
t.@o OF I T E F"FiT 10 t-1
It-iTEF-CEFT k.R.@
LDP E fB)
CHI
FOR
LINERFITY
CHI :S.CiFOR P R D P f;1;1 L I T Y
7.
HETtF-.DGEt-4 I ETY FFiCiTOF-- fk"
@-.4-c@9-t-44
G
5.140073
G
i--,F;EF-i-rEFT-HPT-@ 19 THF C:Elr-,F.L Ir,!IT@ F4F-E t4-0.T I,EF ItiED
P C T. LDt,iF-FC,-Ot-iF.LIriIT = PC:* T. LIPPER C,.Dt-iF.LIr-!!T =
HO. OF COt-iC,.FOR INF;LIT DRTFi-C-ONC.@
1092092
IOOP20!,6 50CIP 20P 20
CLIR@.,E2
3
NO. EXPDS Eli - t4Ej.EtERli
C.Orlt-IENTS FOR CURVE 2 48 HF' RUN Fi
C Dt-ii--
LDG C.Ot-iC-.
f.Fii F-.HI-,:0-: FPDBRE!
2
IL I T@-(;<. 0. Ct5
F F-.'FC,I.OTN F,E *-P-,0: t-D4 I t-,f---
0.1
ci.
FIITED
FRFIC@T I Dt-i
ri
t-iD OF I TEF;,@iTI Cit-I
I r-iTEF,C.EF-,T k R*'- i
5 15 1
H E.T E F-D -iCE C
IF -,v -tl'F
(H-, -t
LDPE (p,"
CH I
F EiR
L'I t-E4 F-F4--I T Y
1
7. 774 C'7 1;@
7.774fli-,l
G
'rHE COt-ii:. L It-IIT'--,F4 E
CHI
FDR,
PROFFIBILITY
(I.Cii.1- CiCi1 4@--@
I
F'F'DE.I T
FEIF;- E:I D
CiFCF COt
FEIF-.-
1 (1,4 CI!4, I ijCi!.4 Ci@ I 5C)O@ 4CIg 4(1
CDr-ir,iEr@T FOR !--'FL-i@,i@E I
CotipIriED 4'-:H.F'.'PUt-iR&B
LOG c 0 ri
1
5 Cici CHI-SO
2
2. 6':-@S97 PF'OPREILIT"f@ < 0.05
liC-T F-t
FRRC.T I Ot-4 RESPONDING
0.1
0.3
1
FITTED FR.FIC.TIOti
0.0388795
0. 5097669
0.8987511
r-(i-j0 i= ITER-H@TIOti-7
INTERCEPT
3. -r1_5,
@4ETEF@DF--Et4l P-T'-r r-PCTDF-- ik"@, :7*11-iCE"f---i C-F@ER.TEF-.- TH,-t-@
L 0PE J"E:
CHI '-@O FOR LINERPITY
C-H I SO FOR PROBF-FP I L I T-f'
1.78',-,32
1!!@.54,'i,C,i9
S. (1451 @:'7E-C,5
15.54,=,,Ci,;
G = SE;82.549
TI-E C:Cit-ir.LIrfiI-i,7F-4F--tE-iDT E,EFIt-ipIj
'7r4-, r-17-T. LCjkkF-F-.
Lltii-T =
';5 PC.T. LIPPEF., C.Ot-4F-. LIr-ill=
USED
PROBIT ANALYSIS PROGRAM FOR CALCULATING EC VALUES
Version 1.4
FC-143 Da.ohnia maona acute static
48-nrs exoosure
Conc.
Number Exoosed
Number Reso.
Observed Proportion Responding
Adjusted Prooortion Responding
10.()0()0
40
100.0000
40
500.0000
42
1000.0000
40
4
0.1000
12
0.3000
42
1.0000
40
1.0000
0.1000 0.3000 1.0000 1.0000
Predicted Proportion Responding
0.0306 0.5327 0.9262 0.9792
Chi - Square Heterooeneitv
19.408
WARNING
Sionificant h6terooeneitv e@fists. The results reoorted +or this data set mav not be valid. The results should be interoreted with apprODriate caution.
NOTE
Slooe not sioni+icantly different from zero. EC fiducial limits cannot be comouted.
ML( Sigma
1.959058 0.511587
Parameter
Estimate
Std. Err.
95% Confidence Limits
-------------------------------------------------------------------
Interceot SloDe
1.172584 1.9547oO
1.712521 o.77@,:.273
-6.196393, -1.368389,
8.541559) 5.2777E39)
Theoretical Spontaneous Response Rate O.C)OOO
BURLINGTON RESEARCH. INC@.
TRIMMED SPEARMAN-KARBER
METHOD.FOR CALCULATION
OF
ECSO AND LC50 VALUES IN BIOASSAYS
FOR REFERENCE. CITE
M.A. HAMILTON, R.C. RUSSO, AND R.V. THURSTON,
TRIMMED SPEARMAN-KARBER
METHOD FOR ESTIMATING
LETHAL CONCENTRATIONS
IN TOXICITY BIOASSAYS.
ENVIRON. SCI. TECHNOL. 11(7) /@14-719
.CORRECTION 12(4) 417 (1978).
1977. MEDIAN
DATE
5/31/82
TEST
1
.CHEMICAL
FC-143
SPECIES
DaDhnia magna
DURATION
48 holtrs
RAW DATA
CONCENTRATION(ma/L)
10.00 100.00 500.00 1000.00
NUMBER EXPOSED MORTALITIES
4t)
40
42
4C)
4
12
42
40
SPEARMAN-i,:ARBER TRIM
10.00
SPEARMAN-KARBER
ESTIMATES
eC50
125.7965775
95% LOWER CONFIDENCE
86.29
95% UPPER CONFIDENCE
lB3.39
-------------------------------------------------------------------------------
8URLINGTON RESEARCH. INC.
Trimmed SDearman-Karber Method for Calculation of EC50 and LC50 Values in Bioassavs
6.907E
x
3
.3
3
x
6.044E
.3
.3
.3
5.IBOE
L
.3
N
3
.3
x
0 4.317E
N
.3
c
3
E
3
N
3
T :7,4.5::,E
R
.3
A.
.3
T
.3
2.590E
N
3
x
.3
.3
1.726E
3
3
.3
.3
.867.4E
.3
3
3
3
ODDDDDEDDDDDDDDEDVDDDDDDDDDDDDEDDDDDDDDDDDDDDEDDDDDDDDDDDDDDE
LCIO
LC25
LC50
LC75
LCLOO
MORTALITIES
FC- 143 Da.ohnia magna acute static
48-hrs exoosltre
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit 10+
a
C)
9+
E3+
7+
.5+
...
0 4+
3+
1+
----------------------------------------------------------------------
ECOL
EC10
EC25
EC50
EC75
EC@?0
EC99
FC-143 Daphnia maana acute static
49-hrs eXDosLtre
Estimated EC Values and Confidence Limits
Point
Conc.
Lower
Up.oer
95% Confidence Limits
EC 1.00 EC.5.00 ECIO.00 EC15.00 EC50.00 EC85.00 EC90.00 EC95.00 EC99.00
5.8607 13.0782 20.0635 26.7824 90.7942 307.7991 410.8741 630.3306 1406.5795